LOCUS       DAELNY010000001        76186 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.001, whole genome shotgun sequence.
ACCESSION   DAELNY010000001 DAELNY010000000
VERSION     DAELNY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 76186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.001"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(186..350)
                     /locus_tag="KPE55_000001"
     CDS             complement(186..350)
                     /locus_tag="KPE55_000001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491107.1"
                     /translation="MKSLLNSTLISSGLIATMFISSSIKSIVPIKSATPRANLNLNNS
                     SLVKYSYFQF"
     gene            560..1627
                     /locus_tag="KPE55_000002"
     CDS             560..1627
                     /locus_tag="KPE55_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0491108.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1819..2349
                     /gene="rnmV"
                     /locus_tag="KPE55_000003"
     CDS             1819..2349
                     /gene="rnmV"
                     /locus_tag="KPE55_000003"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG0491109.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2520..3389
                     /gene="rsmA"
                     /locus_tag="KPE55_000004"
     CDS             2520..3389
                     /gene="rsmA"
                     /locus_tag="KPE55_000004"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG0491110.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3455..4717
                     /locus_tag="KPE55_000005"
     CDS             3455..4717
                     /locus_tag="KPE55_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491111.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4868..5983
                     /locus_tag="KPE55_000006"
     CDS             4868..5983
                     /locus_tag="KPE55_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0491112.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6169..7065
                     /locus_tag="KPE55_000007"
     CDS             6169..7065
                     /locus_tag="KPE55_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0491113.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7135..7932
                     /locus_tag="KPE55_000008"
     CDS             7135..7932
                     /locus_tag="KPE55_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491114.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7987..9339)
                     /locus_tag="KPE55_000009"
     CDS             complement(7987..9339)
                     /locus_tag="KPE55_000009"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG0491115.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9558..10436
                     /gene="purR"
                     /locus_tag="KPE55_000010"
     CDS             9558..10436
                     /gene="purR"
                     /locus_tag="KPE55_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG0491116.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10592..10870
                     /gene="spoVG"
                     /locus_tag="KPE55_000011"
     CDS             10592..10870
                     /gene="spoVG"
                     /locus_tag="KPE55_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG0491117.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11083..12462
                     /gene="glmU"
                     /locus_tag="KPE55_000012"
     CDS             11083..12462
                     /gene="glmU"
                     /locus_tag="KPE55_000012"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG0491118.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12557..13507
                     /locus_tag="KPE55_000013"
     CDS             12557..13507
                     /locus_tag="KPE55_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG0491119.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14345..14430
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14592..15113
                     /locus_tag="KPE55_000014"
     CDS             14592..15113
                     /locus_tag="KPE55_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0491120.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSTTTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            15264..16940
                     /locus_tag="KPE55_000015"
     CDS             15264..16940
                     /locus_tag="KPE55_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0491121.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16952..18160
                     /locus_tag="KPE55_000016"
     CDS             16952..18160
                     /locus_tag="KPE55_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0491122.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSIKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18175..19887
                     /locus_tag="KPE55_000017"
     CDS             18175..19887
                     /locus_tag="KPE55_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG0491123.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19880..20788
                     /gene="pilO"
                     /locus_tag="KPE55_000018"
     CDS             19880..20788
                     /gene="pilO"
                     /locus_tag="KPE55_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG0491124.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20815..21381
                     /locus_tag="KPE55_000019"
     CDS             20815..21381
                     /locus_tag="KPE55_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0491125.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21418..21945
                     /locus_tag="KPE55_000020"
     CDS             21418..21945
                     /locus_tag="KPE55_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0491126.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21966..23504
                     /locus_tag="KPE55_000021"
     CDS             21966..23504
                     /locus_tag="KPE55_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491127.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23600..24682
                     /locus_tag="KPE55_000022"
     CDS             23600..24682
                     /locus_tag="KPE55_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG0491128.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24663..25445
                     /locus_tag="KPE55_000023"
     CDS             24663..25445
                     /locus_tag="KPE55_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0491129.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKKLLELYFSMVI"
     gene            25547..26197
                     /locus_tag="KPE55_000024"
     CDS             25547..26197
                     /locus_tag="KPE55_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0491130.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKVFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26211..26771
                     /locus_tag="KPE55_000025"
     CDS             26211..26771
                     /locus_tag="KPE55_000025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG0491131.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITNCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26788..30174
                     /gene="mfd"
                     /locus_tag="KPE55_000026"
     CDS             26788..30174
                     /gene="mfd"
                     /locus_tag="KPE55_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG0491132.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30205..31200
                     /locus_tag="KPE55_000027"
     CDS             30205..31200
                     /locus_tag="KPE55_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0491133.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31324..31863
                     /gene="spoVT"
                     /locus_tag="KPE55_000028"
     CDS             31324..31863
                     /gene="spoVT"
                     /locus_tag="KPE55_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG0491134.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31983..33596
                     /locus_tag="KPE55_000029"
     CDS             31983..33596
                     /locus_tag="KPE55_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0491135.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33703..35151
                     /gene="mazG"
                     /locus_tag="KPE55_000030"
     CDS             33703..35151
                     /gene="mazG"
                     /locus_tag="KPE55_000030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG0491136.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35240..35518
                     /locus_tag="KPE55_000031"
     CDS             35240..35518
                     /locus_tag="KPE55_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG0491137.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35590..35832
                     /locus_tag="KPE55_000032"
     CDS             35590..35832
                     /locus_tag="KPE55_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG0491138.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35894..36148
                     /locus_tag="KPE55_000033"
     CDS             35894..36148
                     /locus_tag="KPE55_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG0491139.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36149..36682
                     /locus_tag="KPE55_000034"
     CDS             36149..36682
                     /locus_tag="KPE55_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491140.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36688..36972
                     /locus_tag="KPE55_000035"
     CDS             36688..36972
                     /locus_tag="KPE55_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG0491141.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37085..38005
                     /locus_tag="KPE55_000036"
     CDS             37085..38005
                     /locus_tag="KPE55_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG0491142.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDLIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38095..40467
                     /gene="spoIIE"
                     /locus_tag="KPE55_000037"
     CDS             38095..40467
                     /gene="spoIIE"
                     /locus_tag="KPE55_000037"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG0491143.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVESIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40818..42710
                     /gene="pepF"
                     /locus_tag="KPE55_000038"
     CDS             40818..42710
                     /gene="pepF"
                     /locus_tag="KPE55_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0491144.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42824..43789
                     /gene="galU"
                     /locus_tag="KPE55_000039"
     CDS             42824..43789
                     /gene="galU"
                     /locus_tag="KPE55_000039"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0491145.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44289..45893
                     /gene="rho"
                     /locus_tag="KPE55_000040"
     CDS             44289..45893
                     /gene="rho"
                     /locus_tag="KPE55_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG0491146.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46046..46246
                     /gene="rpmE"
                     /locus_tag="KPE55_000041"
     CDS             46046..46246
                     /gene="rpmE"
                     /locus_tag="KPE55_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG0491147.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46391..47284
                     /locus_tag="KPE55_000042"
     CDS             46391..47284
                     /locus_tag="KPE55_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG0491148.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47302..48150
                     /gene="prmC"
                     /locus_tag="KPE55_000043"
     CDS             47302..48150
                     /gene="prmC"
                     /locus_tag="KPE55_000043"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG0491149.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48194..49258
                     /gene="prfA"
                     /locus_tag="KPE55_000044"
     CDS             48194..49258
                     /gene="prfA"
                     /locus_tag="KPE55_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG0491150.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49387..50067
                     /locus_tag="KPE55_000045"
     CDS             49387..50067
                     /locus_tag="KPE55_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG0491151.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50096..51136
                     /locus_tag="KPE55_000046"
     CDS             50096..51136
                     /locus_tag="KPE55_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG0491152.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51153..51602
                     /locus_tag="KPE55_000047"
     CDS             51153..51602
                     /locus_tag="KPE55_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG0491153.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51619..52071
                     /gene="rpiB"
                     /locus_tag="KPE55_000048"
     CDS             51619..52071
                     /gene="rpiB"
                     /locus_tag="KPE55_000048"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0491154.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52107..52736
                     /gene="upp"
                     /locus_tag="KPE55_000049"
     CDS             52107..52736
                     /gene="upp"
                     /locus_tag="KPE55_000049"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG0491155.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            53037..53852
                     /locus_tag="KPE55_000050"
     CDS             53037..53852
                     /locus_tag="KPE55_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG0491156.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54078..54515
                     /locus_tag="KPE55_000051"
     CDS             54078..54515
                     /locus_tag="KPE55_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG0491157.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54700..54927
                     /locus_tag="KPE55_000052"
     CDS             54700..54927
                     /locus_tag="KPE55_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG0491158.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54932..55318
                     /locus_tag="KPE55_000053"
     CDS             54932..55318
                     /locus_tag="KPE55_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG0491159.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55321..56025
                     /gene="atpB"
                     /locus_tag="KPE55_000054"
     CDS             55321..56025
                     /gene="atpB"
                     /locus_tag="KPE55_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG0491160.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56094..56354
                     /gene="atpE"
                     /locus_tag="KPE55_000055"
     CDS             56094..56354
                     /gene="atpE"
                     /locus_tag="KPE55_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG0491161.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56477..56974
                     /gene="atpF"
                     /locus_tag="KPE55_000056"
     CDS             56477..56974
                     /gene="atpF"
                     /locus_tag="KPE55_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG0491162.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56971..57516
                     /locus_tag="KPE55_000057"
     CDS             56971..57516
                     /locus_tag="KPE55_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG0491163.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57533..59035
                     /locus_tag="KPE55_000058"
     CDS             57533..59035
                     /locus_tag="KPE55_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG0491164.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59063..59941
                     /gene="atpG"
                     /locus_tag="KPE55_000059"
     CDS             59063..59941
                     /gene="atpG"
                     /locus_tag="KPE55_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG0491165.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59954..61348
                     /gene="atpD"
                     /locus_tag="KPE55_000060"
     CDS             59954..61348
                     /gene="atpD"
                     /locus_tag="KPE55_000060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG0491166.1"
                     /translation="MANVGKVVQIVGAVLDVKFDGEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61351..61611
                     /gene="atpC"
                     /locus_tag="KPE55_000061"
     CDS             61351..61611
                     /gene="atpC"
                     /locus_tag="KPE55_000061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG0491167.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            62073..62453
                     /locus_tag="KPE55_000062"
     CDS             62073..62453
                     /locus_tag="KPE55_000062"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG0491168.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            62468..62929
                     /locus_tag="KPE55_000063"
     CDS             62468..62929
                     /locus_tag="KPE55_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0491169.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            62950..63540
                     /locus_tag="KPE55_000064"
     CDS             62950..63540
                     /locus_tag="KPE55_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491170.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            63555..64712
                     /gene="alr"
                     /locus_tag="KPE55_000065"
     CDS             63555..64712
                     /gene="alr"
                     /locus_tag="KPE55_000065"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0491171.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            64745..65032
                     /locus_tag="KPE55_000066"
     CDS             64745..65032
                     /locus_tag="KPE55_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG0491172.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            65001..65384
                     /locus_tag="KPE55_000067"
     CDS             65001..65384
                     /locus_tag="KPE55_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG0491173.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            65555..66382
                     /locus_tag="KPE55_000068"
     CDS             65555..66382
                     /locus_tag="KPE55_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0491174.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            66375..67316
                     /locus_tag="KPE55_000069"
     CDS             66375..67316
                     /locus_tag="KPE55_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0491175.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            67740..68834
                     /locus_tag="KPE55_000070"
     CDS             67740..68834
                     /locus_tag="KPE55_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491176.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            69060..69476
                     /locus_tag="KPE55_000071"
     CDS             69060..69476
                     /locus_tag="KPE55_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491177.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(69658..70227)
                     /locus_tag="KPE55_000072"
     CDS             complement(69658..70227)
                     /locus_tag="KPE55_000072"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG0491178.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            70381..71538
                     /locus_tag="KPE55_000073"
     CDS             70381..71538
                     /locus_tag="KPE55_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG0491179.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            complement(71721..72875)
                     /gene="nagA"
                     /locus_tag="KPE55_000074"
     CDS             complement(71721..72875)
                     /gene="nagA"
                     /locus_tag="KPE55_000074"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0491180.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNNTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYIPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICMYKVNK"
     gene            complement(72958..73683)
                     /locus_tag="KPE55_000075"
     CDS             complement(72958..73683)
                     /locus_tag="KPE55_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0491181.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITTGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            73950..74879
                     /gene="pfkB"
                     /locus_tag="KPE55_000076"
     CDS             73950..74879
                     /gene="pfkB"
                     /locus_tag="KPE55_000076"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0491182.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNTCRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(75008..75925)
                     /locus_tag="KPE55_000077"
     CDS             complement(75008..75925)
                     /locus_tag="KPE55_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0491183.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
BASE COUNT        30405 a         8268 c        13930 g        23583 t
ORIGIN      
        1 attttaattt cattattaaa gtatagatgt tatatataaa gtaaataaga tattaaataa
       61 ttataaacta aagtaaataa aatattgagc gattattaaa aagatatata tataatttaa
      121 tttgagcagt ttgttatata ttataaaaaa agagaaaaat aaaaggtaaa ttgtttctgg
      181 ataacttaaa attggaagta agaatattta actagagaag aattatttaa gtttaggtta
      241 gctctaggag tagctgattt tataggaaca atagatttta tggatgatga tataaacatg
      301 gtagcaatta gaccagaaga tataagggta gaatttaata gagatttcaa agaaattata
      361 gacctcaaac tcagaccgta tcgatataaa aactaaagtt agatgatata gtgttgtaga
      421 taggaccatc agctgttatg gcagtattag tgcttttaac aaatataata gtaaaacttt
      481 tgtatgaatt gataataaaa aaattaaaaa ccaagacata aaaatggcaa caaaaagaag
      541 ttgaataagg gaggtaaaaa tgaaatttaa gaagttagtc tctttgggaa tattatcaac
      601 acttttattg agtgtaataa ctggatgtag taatgtttca tcaggttcaa acttaaagtc
      661 taataataag acaaaaggag agttaacagt ttatacagca attgaggaag atagtataga
      721 tacatactta gctacattta aagagaaata tccagatatt aaattgaatg tacttagatt
      781 atcagtagga ggtataacag caagattatt agcagaaaaa gataatccta aagcagatgt
      841 gttatggggt gttgcagcta caagtttgat aattttagat gacaaagaaa tgctagagcc
      901 atacgctccg aaaggctttg ataaggtaaa ttctgcattt aaagatgata agaaagttcc
      961 ttcttgggtt ggaatagatg cttggatgac tggtataaca gtaaatacta aagaattaaa
     1021 agctaaaaat atatcagctc ctagttctta tgaagattta ataaagccag aatataaaga
     1081 tcttatatct atgcctagtc cagcttcttc aggaacagga tatttaacag tatcagaact
     1141 catacaaatt atgggtgaag aaaaagcatg gcagtatatg gataaattgc ataaaaatat
     1201 tggtgtatac actcagtctg gttcagctcc agctatatta gcagcttcag gagaatatcc
     1261 cattggtata tcttttggaa atagaggtat aaagttaaaa ggtgaaggtt atccagtaga
     1321 agtagtattt ccaaaagaag gttctggatg ggagatagaa tcaaatgcac ttgtgaaaaa
     1381 agataatata aaagaagcat caaaagtatt tttagattgg gctataagta aagatgcaat
     1441 gaatgagtat tctaagaatt ttgcagttac tacaattgac acgggaaatc cggtgccaga
     1501 aggatttcca aagaatccat tagaacagat gatagataat gatttaaaat cagcagcaag
     1561 aaatagagag gctatactaa ataaatggat agaaaaatat gatggaaaga cagaaaaaga
     1621 gagctaagcc ttgcaataaa atttagtaca aaaagcgcct agaatataag ttttatatct
     1681 aggctctttt gatatattta tttaaaatgc atatttctat ataaaaataa atagtttagt
     1741 accttgatta aattaaataa aaaccttaaa ataaagagat gtaaagaaaa actaaaaacg
     1801 aataaaaggt gaatttatat gataaaagaa ataatagtag tagagggtag agatgacgtt
     1861 acagcagtta agagagcaat agatgcagaa cttataacta caggtggatt tggatttcca
     1921 aaaggtgtaa tggagcgtat aaaagctgct aatgaaagac gtggtgttat aatatttaca
     1981 gacccagatt ttgcaggtga aaaaataaga aaaaaaatag catcagaagt tccaggatgt
     2041 aaacatgcat ttctaccgag agaggaagct aaaaaggatg gagatatagg tattgagaat
     2101 gctactcctc aaagtataat agcagctctg aataaagtta gaactgaaaa tacagaaaaa
     2161 agagatgaat ttaagcaagt tgacttaatt agaaatggtc ttataggtaa tgaagaagca
     2221 tcacttagaa gagatgcact tggaaaaata cttggaattg gatatggaaa tgcaaaacaa
     2281 tttttaaata gattgaataa ttatggagtt gaaagagaag aatttataga tgcattaaaa
     2341 caaatctaaa cgtaatgttt aatgtatgaa aaatatgtat ataaaataaa ttgtggaatt
     2401 acggttaatt taagatgaat tatttataat atacacctta ttttaaatca atgtaaatac
     2461 atataaataa gtataattaa taaaattaaa tatcaaaata agttaaaaag gagtaaataa
     2521 tggatagact atcttcacat aatgcaacaa aggaagtagt acaaaaatat aattttaaat
     2581 tttcaaagtc tttaggtcaa aatttcttga tagatagtaa tataattgat aaaatcttat
     2641 ctggagctag aatcacaaga ggagataata tcatagaagt aggacctggt ataggaactc
     2701 ttactcgtga gatgggtaaa atagcagaaa aagtagtggc tatagaaatc gatagaaatt
     2761 taattcctat attgaaagat accttgtcag atttagataa tacagaggtt gtaaatcaag
     2821 atatactaaa agtagacata caggaattag taaaagataa attaaatggt gggccagtta
     2881 agcttgtagc aaatcttcca tattatataa ctactccaat agttatgaaa tttttagagg
     2941 aagacatacc tgtaacagac atagtagtca tggttcaaaa agaagttgca gatagaatga
     3001 atgctattcc gggtactaaa gattatgggg cattatctat agctgttcaa tactattgtg
     3061 atacagagat tgtggcaaaa gctccaagac atatgtttat accacaacca aatgtagatt
     3121 ccacagttat aggtctacat gtaagagata aaagaaaata tgatgttcat aatgaagaca
     3181 tattttttaa aacagtaaaa gcatcctttg gacaaagaag aaaaactctt cttaattcgt
     3241 tgggaggtct tggttttctt aataaagatg aaataagaga agtattaaaa gaagctaata
     3301 tagacgaaaa aagaaggggt gaaactctta gtatagaaga gttttcagtt ctttcaaata
     3361 taataaatac aaaagtatct tctaaataga aggtactttt tttaattttg ttaacatata
     3421 ttgtttagag aaactgtgtt aaggggggat tgcagtgggt tctaaaagaa agtttaaaag
     3481 agactatata atactagaag ctaaagacat aaactttaga tacaaagaac gagtactgcc
     3541 aaaagctttt gcaaaagttg aagtaaatga tgaaaaagca attattgcat tatatgtaga
     3601 aaatctaaaa tatgttaaag atgggtataa agcagtagca ataaaagatg accatggatt
     3661 aatagactta ggaaatataa ttttaaatga acaaggaaga ggtgaatttg ttttagattt
     3721 agaagattat gatgatgaga taaaaggtat tgcattattg tatgaaagaa atataccttt
     3781 aattggattt aaaggaaata agcttgaaaa ttatgaggac atattatttc aagaggtaga
     3841 ggaagaagaa tatgaagaat atgaagagtt ggaagaagaa gacgaagaga tagaggagaa
     3901 tgaggaaagt gatagagacg aagatagtga agaagaatat attatagaag aatatgatga
     3961 agatgagtat gaagactatt gggaagatga tgaagatatt gaagaaggag agttaaaagc
     4021 aagaacggaa gtagaagatt ctgatgatga ggaaatagaa gagttcaata attataagga
     4081 aatagaaaat tataataggg ttaaagtaca ggaagaatat atagacatag aatatgatga
     4141 agaagaagag gaagaagaag tttttgaagt aaatagagaa gaagataggt ttgtttcctc
     4201 aaaacctact aaatcaaaga aagttcaaaa agatcagaat aatattgaaa aaataaaaag
     4261 agctaatgga gtaagtcaag gagagagtgg aaataaacaa accagtacat tacttatgcc
     4321 aagacaaata aaaaaaggtt tgaaattatt taaagaagta aaaccttttg taacagatta
     4381 tatagataag acaagatggt ggaaaataga gataaatcct acaacactgt gtggatatac
     4441 aatgccttat ttaggatatt taaacgtatt gaattacact atgtatagtg atgcagtttt
     4501 acattcatat aaatacagac actatttatt tggagttcag tatgatgaat ataataagag
     4561 aaaacattat atttatgcta tcccaggaaa tagaaatgaa cagccagaca atggaagcac
     4621 aggattttta agatatcaag cttgtgataa tagaacaaat agtttaggat attggctatg
     4681 ttttatagat tctagacaaa gaaatatagt taggtaaaaa taataaaaat gaaaaaattt
     4741 agactcaatt cttattttac tgaaaaaatt aatgaaatgt agtacaaaac tttaaaaact
     4801 attgataata tttaaatatt acactacaat atatcttgaa atacctatta tattatggag
     4861 gaaaattatg ataaacgagc aaagaatttt aaatgagttt cttgaattag tacagataga
     4921 tagtttatct ttaaaagagg ggaatgttgc taaagtatta gttaaaaaat tagaagaaat
     4981 aggttgttca gtagtaatag acaatgctgg tgaaaaagca aatggagaaa ctggtaatat
     5041 aatagctaca ttaaaaggaa ataaagaagg aaagaaaata ttatttagtt cacatatgga
     5101 tacagtaact ccaggtatag gggtaaaacc tatagtggat gaagcaaatg gaataataaa
     5161 aagtgatggt actacaatat taggttctga tgataaagca ggtatagcag ctatattaga
     5221 aggcttaaga tatataaatg aaaataacat agaacatact gatatacaag ttgtattctc
     5281 tatatgtgag gaatgtggat tagttggagc taaaaattta gactatggaa aaatagattc
     5341 tgaatatgca tttatattag atagtggtgg ttctccaggt gaaataatag taaaagctcc
     5401 tgcacaagat gtaataaatg ttaagatatt aggaaagact gctcatgcag gtcttgaacc
     5461 tgaagctgga ataagtgcta taatggtagc tgccagagca atagaaaata tgaatttact
     5521 tagaatagac gaagaaacaa ctgctaacat aggaattata aacggtggaa cagctacaaa
     5581 tatagtaaca ggagaagtta atatagttgc agaagccaga agtctaaaag aaaataaatt
     5641 agatgttcaa actaaacaca tggtagagac ttttgaaaaa gctgcgaaag attttggagc
     5701 tcaaattgag atagatgtta atagagcata cacaccaata gatgttagtg aggattctga
     5761 aataataaaa cttgcaaaga aggctttttc aaacttaggt atagaaggtc atactgaatc
     5821 tacaggagga ggtagtgata caaatatatt aagtaagaat ggtatcgaag ctataacttt
     5881 aggaattggt atgaaaaatg ctcatacact aagtgaacat atagctataa aagacttata
     5941 tgattctgct aaaatggtag tggagataat aaaggaagca taaatttaaa taatagtgta
     6001 taatatatat atcgcctagc ttaagctagg tgatttttca ttttatagaa gattgataat
     6061 ctctattgaa agcaaagagt atacatttgc atataagaga gtgtttaatt tatgttcaaa
     6121 tgtaggttct taataatatt tagattgttt cataagggag gaataaatat ggaaacaagg
     6181 tatgaagaag caaataaaat aacaatacaa tcaatacttt ggaatatagt attaacaata
     6241 attaaggtta tagctggtgt tattggtaat tctagcgcta tgattgcgga tggattacat
     6301 tcagcatcag atattataag ttctatagga gtactaatag gcaattatgt atcttctaga
     6361 cctggagata gagagcataa ttatggtcat gagaaagcag agacattagt atcatttgtg
     6421 ttatcaatat tgcttatttt cgtttctata acaataggta tagaggcaat taaatcttta
     6481 tttaacttag atgcattaag tgttccatca atattaccat tagtagtatc agttatttca
     6541 atcctgataa aagagtatca atatagaata acaataaaag ttgctaaaaa gataaattca
     6601 ccagccctaa aagctgatgc ttggcatcac aggtcagatg ctttatcttc agtagctgct
     6661 tttataggta taggtggttc tattttaggg tttaaaccat tagacccaat tgcgtcagtt
     6721 gtagtggcta tatttgtagc taaagtagga attagtatac ttataagttc tgttaatgaa
     6781 ttgatggatg tttcagttga tgaagaagag ataaaagaat taaaatttat tgtagcagat
     6841 acagaaggtg ttaaaaatct tggtgatata aagactagaa aacatggagc tatggcatat
     6901 gtagatttga ctatatgtgt ggatgaaaat ttgacagtta agcaaggtca tgatattgct
     6961 accaagcttg aaaaacatat aataaaacat atggaatttg taaaaggtat aacagttcac
     7021 gttgaaccat gtactaattg ccaaggaaat aagtgtaata attaattaaa acttaaaaaa
     7081 aagtgtaaaa agttatataa ttgttttatg attataaact ctaggggtgg ttaaatgagc
     7141 aagaaaagaa aactaaagaa aaaagctgca atattaatag gagtagttgt tttctttata
     7201 atatacttta tatcctttag aataggaata aatatggaaa aggataaaag tgaagtgcca
     7261 aagaaggtag aaaaagaaga agaagataat aggtctttaa ggactcaaat aaaagagaag
     7321 aagaaggttt atatatcaga taaaaatata gaaaacataa aaattgaaga agatatgtgg
     7381 gatgaaataa agttattttt ctcagatttt aagaaagtaa gaaatactac ttcttcttac
     7441 gaagcagttt atgaaggtta ttctgatgat ggagttaaat tttctactga ccttaacttt
     7501 tttagagtat atactgtaaa taaagaagaa tattataaag taccagttag ttctaaggat
     7561 gcttttaaga aacttttaga tgagagtata tatacttcgt ttgacctttt aagtcattat
     7621 aaagattgga aagaggttac tataactcat ggaaaagaaa ttaaaaaagt tcatagatgg
     7681 aaatttgatg atttagcaca taagatgatt tcaaagaggg ttgtaggaaa agttcaacct
     7741 gagaaaagta aggaaagaag tgactataat tttactataa atataaaagg tgataatttt
     7801 gatgtaaaag ttgatacaat gggaaaagat tatgtaaaag ttacttctaa gaaaaatgta
     7861 tcttactatg aggttcatac aagtttattt gattacctta aaaatgagat atttgaaatt
     7921 ggaaagaaat aagactttaa gaaggctatc aaattagata tttgatagcc taaattattt
     7981 tatatattat ttttcaacca acaagtctgc aaccttataa attggaccag caccagcagt
     8041 tattactaaa tcattatcct taacattttc acgtaagtat tttactatat cttcaaattc
     8101 ttttatatag atggcatcta cattgttttg atataatttc tcaaccaagt ccttagaatg
     8161 tatatctcct gggtcttttt ccctagcagc atatatatct gttatgataa ctttatcagc
     8221 agcatagaat gcctctgaaa attcacctaa aagagatttt gttctagtat aagtatgtgg
     8281 ctggaagata caccataaag ttgatttttt taattttttg gcagcagcta aagttgcttt
     8341 taactcagtt ggatgatgtg cataatcatc tactactaaa gcattcttat agtacccttt
     8401 agtttcaaat cttcttccga cacctttata aatttttata ttttttctta tagtctctaa
     8461 gtcaatacca gaaacaagag ctgccattat ggcagaagaa gcattgtata tgttatgaag
     8521 accatataca gaaagttcaa attctcctaa atctcttcct tcatattcta ttctaaatat
     8581 tccatgacca ctattgtcaa agtgaatatc ttttattaca gcatcattgt cagaatctct
     8641 gccgtatttt ataatagtag ctttaacatc atacaatatg tcatcaatat tttcatcatc
     8701 gccatttatt atgaaatagc cttctggggg taataatttt ccaaatttgt taaaagaagc
     8761 cttgatttca tctattccag agaaataatc aagatggtct tcttcaacat ttaaaactat
     8821 agatatcttt gggttaaaat taaggaaact atctacatat tcacatgctt cagttataaa
     8881 gtggttggaa ttacctatct taacattacc acctatcata cttaagttac caccaactag
     8941 tatagttggg tctaagtctg catattcaaa gattgtagat aacatagatg ttgtagaagt
     9001 ttttccatgt gttccagaaa ctgctataga atttttatat tccctcatga tttggcctaa
     9061 aaaagttgct ctgttcatta ccaatttatt tttttcttta gcagccacaa gttcttcatt
     9121 gtctggatga actgctgctg tataaacaac catatttaca tcatctgata tattatcttt
     9181 tttctgacca atataaatag ttgccccttg gtccctaagt ttgtctaata ggtatgattc
     9241 gtttgaatcc gaacctgaaa cttgataacc tttattgata catatttcag ctaatgcact
     9301 catgctgata ccgccaattc ctataaagtg tatattcata ataaaaccac ctttcttttt
     9361 aattaagtcc tacaatatga actatttctg tggtataata atataatgtt caatatttta
     9421 taattaatat gcatataata ttatatgtat tttatttttg aatataataa aacataagta
     9481 atattatacc acaataagaa ttgttatgca ttagttagtt taacgttata taaaggtact
     9541 tgtaggagga aataataatg aaatttaaaa gaacagagag aattggagca atagttaaaa
     9601 tactgtctga taatccaaat aaaatatata ctttaagtta ttttacaaat caatttaatg
     9661 cagcaaaatc aacaataagt gaagatttat tagttgtaaa aaatgtattt gagaagctac
     9721 atttaggtaa agttataact atttcaggag cagcaggagg agtaaaatat atacctaaaa
     9781 cttctatagc agaaaatgca gagttcttaa tggaattatg cgagagaata tgtgacaagt
     9841 ctagaatact atctggtggt tttttatact tgattgacct aatttacgac cctacaatag
     9901 ctgctaagat aggaaagata ttcgcatcaa atattgagta tgtagatgca gattatgttg
     9961 tgacaatgga aacaaaaggt atacctatgg cacttatgac tgcaaaagct atgaatttac
    10021 ctctagttat aataagaaaa gatataaagg tatcagaagg tcctacactt agcatgacat
    10081 atgtgagtgg aaacagttct aaagttgaaa gtatgagttt gccaagaaaa gctcttaaac
    10141 cagatagtaa agttataatt atagatgatt tcatgagggg tggaggtact ataaaaggaa
    10201 tggtagacct tatgaatgaa tttggtgcag aggttatagg tactggagta ttcatatcta
    10261 caacgaatcc ttcagaaaaa atggtaaaag attatatttc attgattcaa ttggatgtta
    10321 atggagataa aatagtagta gaaccaaact taaagacttt taaagatgaa tatagaaatg
    10381 aagatttaga cgaagaagaa gattcagaat ttgaatttga gatagatgaa gattaataaa
    10441 aaaaatattt agaaaaatat gtaaaaataa aaagaggata tccctagttg ttcatagaat
    10501 tatttaaaaa acagtttata ttaatttatt ccagcgataa taattttaat ataaattaat
    10561 ttctgtcaaa atcaaagggg gatattttgg tatgaagata actgacgtaa gagtaagaaa
    10621 attaacagaa gaaggtaaga tgaaatgtat agtttcaata actttcgata atttgtttgt
    10681 agtgcatgat ataaaagtta tagaaggcca taatgggcta tttatagcaa tgcctagcag
    10741 aaaagtaggc gaaggaaatt tcagagatat agctcatcca ataaatgcag aaatgagaca
    10801 agttttagaa gatgcagttt tacaagcgta tcatgaagca ttagttcaat gggaagtagc
    10861 tgctgaataa taataaatat ataaaatgga gagagcccga acatttaggc tctttttaaa
    10921 tgtagaattt ctattaaaat atgtactttt tagtaaaaaa gtgctatatt attagtgata
    10981 atgtatatgt tgtatacaaa ttgttagata aatgtattat aatgtatatt gattaagaga
    11041 ataatacgaa taaaattaaa ttagattgaa tggagtgtat caatgaactt taaagctata
    11101 atccttgctg ctggcaaagg aacaagaatg aagtctaaat acccaaaagt tatacataaa
    11161 gtatgtggaa aagaaatggt taaccatatt atagatgttt caaaaaaatc aggggtaaaa
    11221 gatactgtcg tgattttagg acatgaagct gatgtagtta aagaaaagct tgcagaagaa
    11281 attataatag ccatgcaaac agaacaactt gggacaggac atgctgtaaa gatggctaaa
    11341 gaatatatta atgatgaaga tacaatagtt gtactttgtg gagatactcc acttataaaa
    11401 gaagagacac taaagagact atttgaatat catatagaaa ataaatatca tgctacagtt
    11461 cttacaacaa gagttggaaa tccaacaggt tatggtagaa taataagaga taagaagggt
    11521 gaccttctta aaatagtgga acaaaaagat gcaaattcag aagaaaagat gataagtgag
    11581 ataaactctg gaatttattg ttttaatgga aagagtctta gagaagcact agatttgcta
    11641 aataataaca attcacaagg tgaatattat ttaactgata ctgctaaaat aatgagagat
    11701 aaaggattaa aagttggtgc atttgcaggt tcaactattg aagaacttat gggcgttaat
    11761 tcaagagtag aattatcaaa ggcagaagaa attatgagac gaagaataaa tgaatctcat
    11821 atggtaaatg gagtaactat aatagacact aactctacct atatagaatc tgatgttatg
    11881 ataggcaatg atactataat atatccaggt gttatgttac aaggaaaaac aaggataggt
    11941 tcagattgta taattggaat gaattcatct ataacaaatt cagaaatagg tgatggtaca
    12001 gaaataaaaa attcaactat aattgatagt aaggttggag aaaattctac tgttgggcca
    12061 tatgcatact taagaccaaa gagtgactta ggaaataatg taaaaatagg cgattttgta
    12121 gaagtaaaaa atgctattat agaagatggc tcaaaagcat ctcacctttc ttatatagga
    12181 gatgcacatg tcggtaaaaa tgtaaatatt ggatgtggag tagtatttgt aaactatgat
    12241 ggtaaaaata aatttaaatc aatagtaaaa gacaatgcgt ttataggttc aaattctaat
    12301 ttagttgcac cagtagttgt agaagaaaaa ggatatatag caacaggttc aacaataaca
    12361 catgatgtcc cagatggtgc tttggcaata gcaagagaaa gacaagttat caaagaagga
    12421 tgggtagaaa aaaagaatca aaaagacgac caaagtaagt aattatgtag tttattattt
    12481 tggctaaaaa atttagctcg aaattattat aaattatatt cattagtatt ttaaaatttt
    12541 caggagggtt acataaatga atactagcgg aagcgagatt aaaattattg caggtaattc
    12601 atcgaaagag ttagcacaaa aaatagctga ctatataggt gtatcagtac tagattgtga
    12661 agtaggtaca tttagtgatg gtgaaatatg tgtaaacatg aatgagacag taagaggttg
    12721 tgatgtgttt gtagtgcaat ctactaacag cccagtaaat gataacttaa tggagttatt
    12781 aatcttaatt gatgcgttaa agagagcatc agcaggtaga ataacagcag ttattcctta
    12841 ctatggatat gcaagacaag acagaaaggc taaggcaaga gatccaatca ctgctaaatt
    12901 agttgcaaat ttaataactg ctgctggagc agatagagta ttaacaatgg atttacatgc
    12961 agctcaaata caaggatatt ttgatatacc attagaccac ttactaggtg gaacaatatt
    13021 agcaaattat tttaatgaaa agaaaataga agatttagta gtagtatctc ctgacttagg
    13081 aagtgttact agatcaagaa aatttgctaa tactttaaat ggagaagttc caatagctat
    13141 aatagataaa agaagaccga aagctaatgt atgtgaagtt atgaacttaa taggtgatgt
    13201 taaaggtaaa aatgttatac tattagacga tatgatagat acagcaggaa caatagttaa
    13261 tgctgcaaat gcacttaaag agtttggagc aaaagatgtt tatgcttgtt gtactcatgg
    13321 tgtgttatca ggtccagcta ttgaaagaat tgctaattca gaaataagtg agttaatagt
    13381 tcttgatact atacaacttc ctgaagagaa aagaatagat aagataaaga taaaaacagt
    13441 agctccttta tttggagatg ctataagaat gatattctct aatgaatcag taagtaaatt
    13501 attctaattt attgtattta tgagagttgt ttaaaaggat ataaaccttt tgagcaactc
    13561 ttttttgatt ttatacttat ggaatgttat ttttttcttg ttagaaaggt atatattatt
    13621 gtaaaaagag attatagttt aaaattatgt atatttaagt aaaataaacc taataaaggg
    13681 tatattattc aaaatattat gaatataaaa aatattacaa aaaagtattg aaaaaattca
    13741 gattatgtag tattatgtaa atataaataa agaaagaggt gtttccaatg ggaaggttag
    13801 aagatgaacc cctaataatt aaatgattca gtcacaatct aaagtgaata taaaaatcta
    13861 ttgaaagttt atttataaga attgaaaata gtatctattc tatctttagt gtttgtgaga
    13921 gggttctgga ttatataaga ttactgtaaa gagtaatttt gcacaataag catatctgta
    13981 aatgaagagt ttcgtttata taatgattgt ggctgaatca taactataat agatagaatg
    14041 tttataatag atgattttat aaaatactat attgtaatag cttttagctt tcaaataaat
    14101 attagcaaag gatggaaatg acaatgataa aactaaatga taatttataa atatctcctt
    14161 acgatatata ataattgtag ggagatgttt ctttttttgt atagcataat ttttctagaa
    14221 attctgtttc aatatgtaaa aagttggtat atataaatag tataaacagt gttgctaaaa
    14281 tatggtagat attatataat ataagtggat atgtatatac attatgagtt atataatttg
    14341 aatttaataa aatagaaacg ttgatttatg ttctgtaatg tgggcacctt ggagcatatt
    14401 gagttagtgg tgcaaccggc tatgaaattg tatttattta taaatactat tattttcata
    14461 gcttttttta tttaaaaaat ggttatatgt tagaaataaa tttggttagt acgtttaaat
    14521 aattttactt agatttttaa ccagatatat tgtaagtaag tattaattaa aaaatttaag
    14581 ggggaataaa aatgaagtta aaaaagaata aaaaaggttt cactttagtg gaattattgg
    14641 tagtaattgc aattataggt atattagcag tagtggcagt tccagcttta tttagtaata
    14701 taaacaaggc taaggtagca agtgttgagt ctgattatag ttcagttaag agtgcagcat
    14761 tatcttatta ttcagatact aataagatac cagttacacc agatggtcaa actggtttaa
    14821 gtgttttaga gacttatatg gagtctctgc ctgataaagc tgatataggt ggaaaatata
    14881 aattgattaa agttggtaat aaattggtat tacagatagg tacaaatact gaaggagtta
    14941 cattaacaga ggcacaatca gcaaagttat tgagtgatat aggtgaaaat aaaatatata
    15001 caagcactac aacaaatagt ttgggaaatc cattaacaag taatacaaaa atagataata
    15061 acgttctata tatagtactt atagataata ctgtgatgga cacaacaaaa tagttaaaac
    15121 aatattttta taaaaatatt gtaaatttaa tgaaaaatat aatataaaag acaggaagaa
    15181 aatcttcctg tttttttata ctattttctt taatgttcaa ttatgaatga tgatataatt
    15241 gagatagtaa aggagtgaaa cctgtggcta aaaaagttag aataggagac aaactggtcg
    15301 aaaaaggata tataacagaa gaacagctta aatgggcact atcagaacaa aaaaacagtg
    15361 gaaaaagact aggagaattt ctagtacaag aagggcttat agatagtaac ctactaataa
    15421 gtgtattaaa agaactcttg gatatagaaa gtatattttt agaagggaca gaaatagaca
    15481 ctttagctac aaagatggtt ccagaaaata tatgcaaaag atatactgta tttccattca
    15541 aaatagatgg aaataaaata tgtctagcca tgtcagaccc acaagacaga gaagcagttc
    15601 aagatgtaag gcgtatgtca ggtaaagacg tagagatatt tatatcaagt acagaagata
    15661 taaacaaagc aataggtcat gcttatgcac attcagaaat aaataaggca atgactgagt
    15721 acaacaaaaa tagaactggt ggagtaagag aaacagtaat acttgaagaa gatgtaaatg
    15781 ctgctccaat agtacgatta gtaaataaca tactagaaaa tgctgttaga atggaagcaa
    15841 gtgatataca tatagaacaa agtgaaaatt acatgcgtgt taggtttagg atagatggaa
    15901 tgttaagaga atatatgagg atgaactcag caccatataa ggcagtaatc agccgtatca
    15961 agataatgtc agatataaat atatctgaaa agagaatacc acaggatggt agaatctatt
    16021 taaaagtgga taataaaccc atagatttta gggtttctac aatgccaact aacagggatg
    16081 aaaagatagc tatgagggtt cttgataaga gtaattttat ggttagtaaa gaagtactag
    16141 gaatagacga acatggctct aagatatatg atgaattgat aaacactcct tatggtctta
    16201 tattggttgt tggccctact ggaagtggga aaaccactac attgtattca atgttaaatc
    16261 aactaaatac agaaaataga aatctattaa caatagaaga ccctatagag tatgaattac
    16321 ctggtgtaaa tcaatctcaa ataaatgaaa aggcagggct tactttcgcc agcggactta
    16381 gagcttttat gcgtcaagac ccagatataa taatggttgg agagataaga gatactgaga
    16441 cagcagaaat agcaattaga gcttctctta caggacattt agttttaagt acattacatg
    16501 caaatacagc agttggagca atttcacgtc ttttagatat ggatgtggaa agttttttaa
    16561 taacttcatc tgttttaggt gtaatatctc aaagacttac acgaaaaata tgtgagcatt
    16621 gtaaggtttc atatgaagca gatattggag aaaaaaaggc tcttggtatt gatgtaaatg
    16681 aatcagtaac tatttataga ggaaaaggtt gtgaaaggtg taataatact ggatataaag
    16741 gacgtctagg tatatttgaa atgcttgaaa ttactccaga aataaaagag ttaatagatt
    16801 catctgcaaa tcaaagagaa attttaaaga tggctagaaa acaaggtatg gttagcttaa
    16861 aagaggatat agttaaaaag gttttaaatg gaaagacaac agtagaagaa atgattagaa
    16921 taattttgat gactgattaa aggatgatat tatgaaaagt tttaaataca cagtaataaa
    16981 agaggacgga aaaaagcaaa ctgatgttat tgaagccaac gattttaatg aagctagaaa
    17041 tcttcttaga aagagaaatc ttagagttat tgaaatcaaa gaaagcaaaa ataaaaatat
    17101 aagattatct agtataaaga gaaaaaaaga cttaggagct gaccagataa gccacttttg
    17161 tagacaattt gctattatag tatcttctgg tataaatagt atttctggat tagaaacatt
    17221 agctagaagg tcaactaaca ttacattaag agaagaaatt aatcgtatag tagcagaaat
    17281 aaaagtaggt tctactattg cagattcaat gctttcatca aaatcaaagt ttccaaagtt
    17341 acttggtgct atggtagcta caggtgaagc tacaggtaaa ttagatgaag tattaaaaag
    17401 tatggcttca ttttatgcaa gtgaacacag ggttaaacaa aagcttagaa atgctgcaac
    17461 atatccatta atagttttga tttcttcatt tataatgatt tttatattta caacatttat
    17521 ggtgcctaag atgattaatt caattacaac agtaggagca actcttccac ttataacaag
    17581 aattgttatg gggtttggta tgtttatgaa gaagttttgg ataatagtac ttttagttat
    17641 tatattattt atctatcagt ttaaaaaata tctaaaaacg tcagttggaa gggctcataa
    17701 agatagagtt ataaataaga tacctgtttt aggaaaagga ataaattgta tggtagctac
    17761 acgattttca agagcattgt atctatttgt atcaacagga tacccattag ttcaagggct
    17821 tgattatatt atagatagtg taaataacac tatggcagag aagattttag catcagctaa
    17881 agatggaata acaagaggag aaggcttagc agaaaattta gagagatata cgtattttga
    17941 ctctgtgttg gtacagatga tagctatagg ggagcagaca ggggaattag agaatataag
    18001 tagacagatg gcagaatttt atgagtatga atccgagatt tatttaaata ggatggcctc
    18061 aatgatagag cctgttttaa taattgcagt tggtattata gttgcgatac tggttgtatc
    18121 aatattcatg ccaatgctta gtatatatga tgccatgtag gagggaaagg acaaatgaac
    18181 aaggtttatg tatcatatta tggcgattct atttccatag ttgagggcag atataaacac
    18241 gataaggata agttttatgt aaaaaatttt aatgtgtttt caatagagga tattgcacaa
    18301 gatttttcta aggaaaagta tgctctattg agatacgcat tagaaaatgt taagttaaaa
    18361 tcaagacatg tagttttttg tttaaataca agagatgtaa ttttaaaacc ccaaaaactt
    18421 cctaatatgg acaaaaagga tttagacgga tttatgaagc tagagatgga cgaaatgatg
    18481 gctttggagt cagatgaata tgtattttcg tatgaagtat ctagtgaaac acctgataat
    18541 tcagatggcg atgaatcaag cttaaatctt attattggag ctatacaaaa ggaagaggta
    18601 aatgcaattg tagagataat gcatgaattt gatttagtat tagatagaat agatacatta
    18661 tcaacagctt acttaagaat gcttaaaaat ttagactaca atgatataat ggtaaccaat
    18721 ataagtgaca atagcacaat tataaatata tataagaaag atactttatt tattagtgat
    18781 aatataccta taaagataac tgctaaagat atggacatgc aattattaaa tgtagtagaa
    18841 gaatcaaaag gtcttatgaa ttactattcg tcaagaaact ttggtaaggt tacggatact
    18901 atattaatat taggaaaaag atattccaat aaattaattt atgaaacttt tagctctgca
    18961 ttttcaagct atgtttctca aggccttact gagataatgg atatatctga tatgataact
    19021 ggagatatag atgaagatga tataaattca attgtagagc ttttaggaag tatgatagaa
    19081 cctaaaggga aaagagattt tgaaaatata aactttttac caatggatat acttagaaaa
    19141 cagataaaag ctaaacaaat gaaaaacata ctgaaaattg ctccaatagt tattttgatt
    19201 cttgctatac cttatttatg tttaattttt tcaaataatt tagcaaataa ggaacttgaa
    19261 aatgttaatg gtgaaataag aaatattgaa tctgcatatt atcagattgg aaatatagaa
    19321 aaaatgatac agagtaaaag cgaagaaata aaggtatatg atatgttaat tgggaaaaag
    19381 gtaaagtggg aaaatttatt ggatgatata gataaaaata ctccaagtaa agttgaacta
    19441 acaagtttat caacaactta tcaaaatctt ggaactaata ataaagctga taattcaaaa
    19501 agtaataaga ctacaacaga taaatcaagt aacaatgaaa caaaatcaag tacgaaaagt
    19561 gatagtaatg gagataaaac agataatgat ttaaatgaag ataagactac aaatgtgtca
    19621 gaagcattaa ctggaaatag tgagactaat agtaataaca cggataaaga tgataaaact
    19681 ccattgtatg ataagatacc aaatgttata aatatagaag gaaatgctaa agattcctct
    19741 tatgtaggtc aatttttata taaattaaag ggattatact atttcaagga tgttaaatta
    19801 catagcataa aaagtaatgc agaagattct aactatacat ttagtatgac attatacctg
    19861 aaagaaggtg tattaactaa tgaataaaga tatatttaaa aaagatataa aagatatatt
    19921 taaaatggaa gttagtctaa aagggattct tataaaggtt ataattttat tagctatttt
    19981 atcatatggt tattttggac tttaccctca atatcagaaa tatattgcta caaaaaattc
    20041 tgtaaaacag gctactgaaa aattaaatat atataaagag aaaactgcac ttatgccaaa
    20101 atatgaaaaa caacttgaag aagttgaaag tgaattaaaa gaaaaaagca aaaatctttt
    20161 atatgatatg caagatggtc tttttttaat aggtttagat aaaaaaatga aggcttataa
    20221 tattgactta accagttatg acgttggaaa tgttaaagaa tacgaacatt tttatggtat
    20281 atcaacaaca ataagtgtta gaggtgatta tagaaatgtc aaaaaattaa tggcttattt
    20341 agaagaacaa aaaaatgtta ctcaaatact agattataca atgacttcat atatagaacc
    20401 agaaaaagaa gaggaagaaa caacaaacaa taaggattta attaaaaatg tgaacataaa
    20461 taaagataca aaaatatatt gggtggaagg cgaaaataag tggcatttat ataaggattg
    20521 tccattgata aaagagggag agattataaa aaatgacaaa cttttagata ggtctactaa
    20581 ttatctatgt aatacttgtg tacttagagc tgcaacagaa aaagaagcaa aaaaagaagt
    20641 cgtagctcca aaggcagaag gtgtagtaga tgctacattt aaatttataa tgtacactaa
    20701 agagaatact acaatgatac ttgatacaga taatccatct tcatggagac caggaaaata
    20761 taatccattt aagtctagtg cgaaataaag ctaagtaagc tttaaagata ggtgatgagt
    20821 ttgtacaaaa ataatgaaaa aggtcttact ttattagaag taataatagc tgtttttata
    20881 ctgacaatag ttttaagtat ttcttataaa gtgttcaatg gtataacatc agcagtaaaa
    20941 aagcaacaga ttattacaga tgctcaagta aatattaatc taattaataa gtatctaaat
    21001 agagatttgg aaaactgtaa agagctaact aaaactggtt caggtaataa ttatgaatac
    21061 aatatagaga tgccagataa tgttgtaaaa tatgaagttt ctatagaaac taaaaaaaat
    21121 acagaggtat attctgtaac gagaatccaa aacaatacaa ttgatacaga aaatgaagtt
    21181 agagaagaga taatctataa tcaaccacta gttcaaaaca ataaagaaat gaaggaaaca
    21241 ccatttaaaa tagaaaagca aactggtaaa tctatttata ctgtaagtat atactataat
    21301 gaatcagtgc aagaaagtca taaaaatagc aatttaaata acaaaactta tacatttgat
    21361 gttatgtcaa gaataggata ataagaattt attaaaaaat aagtaggttg ataaaatatg
    21421 agtaaaaaga atagtgaaag aggattttcc ttaattgaag tgttagtagc tatggctatt
    21481 atgggaattg tactatttgc attttttaat attatcaata ctaataacaa agcaaacact
    21541 aaaaatgaca cagatataac ttccttaaac tatgtccaaa gtgaaataga aaatctaaga
    21601 gaaaagataa aaagtggaga atttgatttt gatagtttag ataaactaga agatggaact
    21661 gtcgtatatg aaaaattaat agataaatca aaaaaagttg tatatgataa agttcttagt
    21721 gaaggtgacg tgagcttata tgatactcca tatgaaaaga ttacaacaat aaaagatgaa
    21781 gatggtaatt taatcgacaa aggaaatatt acaaataaga taaagactat tgtagaagat
    21841 aaaagtggtc agatatataa aatagctgta actgggaaaa gcatgaatga ctattcaagt
    21901 aaaaaagagg ttaaaattgt aactgaaata tttaaagata aataatttta cttgggggtg
    21961 agattttgag gaagtggaat aaatttaaaa gtgaaagagg ggcagcttta gttttagttt
    22021 tggttgtagt agcattgctt tctattgttg gattgatatt ttcaaatcag atagctaata
    22081 gaataaaatc tacaaagact actaatgaag gtatacaagc aaagtattta gcagagacat
    22141 gtgtggaaaa tagtattgat aaagcatatg aaaaattgta tgatgagctt gagaaaatgg
    22201 ataatgagtt taaaagtgaa aatcaagaaa aatccatatc taggtcaaaa ttaagaaata
    22261 tatcagatga agattttaat aatcaagatg aggaaaatat tgaagcagaa agacttggat
    22321 atatgaataa tataaatttc tatttaaata aagcttcttc agacttggaa aaagctagta
    22381 tggaattgaa aaagctatat gatttagata tgttggacta tagagacatt gaatatgtag
    22441 atgcaaatat tatttcacat agagattcta tcttagaaat ttgcaagaat tatacttctg
    22501 gagatattag taaaataaat gaatatattc tgaaagaaga tatagactca actacattga
    22561 ttgaggcaaa gttagtgaat aatgatattc ttttaaaaaa gtttttagaa gaaaataaaa
    22621 ttgaaaatga acatttaaat tcagcctttt cacatactta taaggcatta gataacattt
    22681 ctttggcgat gcaaaatatg attgagtata gacatacgtt tcatatagat gagccaaaag
    22741 tagaagtatc taatgggatt ccagactctc aacaatatta tgaactaata caaaatccta
    22801 taataaatag tatggagtat agctggaata gtaaatggga tacattagaa aacctattgg
    22861 aaattttacc aaatcaaact caaggattta attctttaag agttcatttg agaaataatg
    22921 taagaaaatt tgagaagtta agcgataata tatctagtgg taaaaaaaat acagcaaaaa
    22981 atttcttaaa gtataaagag ttattgtatg aaatatcaga ccaatgtaat caactaaaaa
    23041 gtatgtctta tgagaaaata cctgttaaat atgataacat ggctttgata acaacttttg
    23101 actatataca aaatgagctt ttagcagaga taaaatgcag attaaaagaa ttaaaaccac
    23161 aagaaattga taaaacagaa ggtattacta taaaaatacc tttttataag gctgactacg
    23221 acatgacaaa agaaggatgg cctaaattaa aagaaaatgg ttcaggggcg gagttaagct
    23281 taatggtaac tggtgataaa aatggaataa aagaagtgga agttacagat ggtaaaaaaa
    23341 atattattgg tttaggtgtt gaagaaaatt caaattcaaa gtataaggtt gacgccattg
    23401 taaactttaa tctaaatata gatacaaatg tagttggaaa ttatgatata aaagataaaa
    23461 ttttgattaa tcatgatata agttcataca aaaaagtaaa ctaaatataa gaagcaactt
    23521 agtataaata acaaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    23581 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    23641 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    23701 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    23761 agcaggggaa tctatgttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    23821 tctaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    23881 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    23941 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    24001 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    24061 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    24121 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    24181 tcaggaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    24241 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag taggggcagc
    24301 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    24361 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    24421 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacctgat
    24481 aagagaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    24541 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    24601 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    24661 caatggatat tataatcgtt aatctatatg tatttatagt gggtatagtg tttggaagtt
    24721 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga ccaccttctc
    24781 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ctaagctatt
    24841 taatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    24901 tggaattaat tacaggactt ttatttttaa cagtatttat gttatatgga atatctacat
    24961 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    25021 accactttat aataccagat aaaatattgg tttttggagc tatattttct atgattttta
    25081 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    25141 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    25201 gaggagatat taaactattt gcaatgctag gcctttacat gggtgttaaa aatggtcttt
    25261 tgacagcctt actaagcgtt tatgtaggag cagtctatgg aatctgtgta ataattataa
    25321 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    25381 gagcattaat aacaatctta tgtggaaaga aattacttga actttatttt tctatggtta
    25441 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    25501 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    25561 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    25621 agaaaagaaa aatttataca atacgttttt tatttacata ataactgtag ttttatctgt
    25681 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    25741 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    25801 agttagtggt ataatagttc aaggtataat ttttttggcc tttttcaata tagctcaatt
    25861 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    25921 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    25981 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    26041 ttttattatt ttatacataa ctaaagtatt taaagatgta gatataagaa agcaaattcc
    26101 ttttacacct tttatttcct tgtcgacaat tttaataata tttacagagt ataatatatt
    26161 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    26221 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    26281 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    26341 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    26401 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    26461 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    26521 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaattgc ctaggttcaa
    26581 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    26641 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    26701 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    26761 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    26821 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    26881 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    26941 cgacaaatga tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta
    27001 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    27061 ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca
    27121 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    27181 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    27241 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    27301 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    27361 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    27421 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctaggga atttatttat
    27481 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    27541 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag
    27601 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    27661 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    27721 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    27781 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    27841 ttaaactcgc tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    27901 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    27961 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    28021 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat
    28081 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    28141 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    28201 gcgtctaaga ctagcaataa aaaagttaaa aagggaaaga aaatagaatc ctttttagat
    28261 ttaagtgttg gagattatgt agtccatgaa aatagtggtg taggtagata cacaggaatt
    28321 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    28381 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    28441 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    28501 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    28561 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    28621 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    28681 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    28741 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    28801 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    28861 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    28921 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    28981 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    29041 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    29101 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    29161 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    29221 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    29281 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    29341 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    29401 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    29461 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    29521 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    29581 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    29641 acagagtttg gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga
    29701 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    29761 aaaatgctta atgatgcaat taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat
    29821 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    29881 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    29941 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    30001 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    30061 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    30121 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    30181 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    30241 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    30301 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    30361 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    30421 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    30481 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    30541 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    30601 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    30661 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    30721 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    30781 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    30841 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    30901 acttcagcaa gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa
    30961 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    31021 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat
    31081 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    31141 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    31201 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    31261 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    31321 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    31381 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    31441 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    31501 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    31561 gattctataa tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt
    31621 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    31681 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    31741 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    31801 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    31861 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    31921 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    31981 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    32041 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    32101 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    32161 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    32221 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    32281 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    32341 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    32401 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    32461 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggct tacttgctta
    32521 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    32581 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    32641 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    32701 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    32761 tagataatgt tattgtaata agaagactta tggaagcagg ctttacatac aaagtggcaa
    32821 attccatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    32881 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    32941 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    33001 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    33061 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    33121 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    33181 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    33241 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    33301 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    33361 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    33421 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    33481 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    33541 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    33601 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    33661 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    33721 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    33781 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    33841 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    33901 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    33961 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    34021 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    34081 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    34141 tcatatatag acttggatac aagtatgata ataactcagg tatatgataa atttatagca
    34201 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    34261 aatggagctg gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    34321 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    34381 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    34441 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    34501 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    34561 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    34621 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    34681 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    34741 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    34801 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    34861 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    34921 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    34981 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    35041 ataaatagat ttgactttat agaaaagagt gcaatgaact tgaataaaaa attagaagac
    35101 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    35161 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    35221 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    35281 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    35341 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    35401 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    35461 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    35521 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    35581 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    35641 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    35701 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    35761 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    35821 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    35881 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    35941 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    36001 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    36061 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    36121 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    36181 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    36241 aagagggaat tttaaattca tcaattattt tgctataata tttgatgtat tgttttggtt
    36301 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    36361 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    36421 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    36481 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    36541 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    36601 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    36661 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    36721 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    36781 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    36841 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    36901 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    36961 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    37021 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    37081 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga
    37141 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta
    37201 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    37261 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac
    37321 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    37381 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    37441 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    37501 agaatttatt gtaggtgatt cagaaggaat taagtttaat aaaagtgaaa atgtaggtgc
    37561 tcttaggatg acagagaggt ttttgaagca cgatcttatt gaagatgaag agtttgaagc
    37621 tatgagttca tttatatatg gtgagattga caaaacgtta aatcatataa aagacagagg
    37681 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    37741 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    37801 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    37861 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    37921 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    37981 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    38041 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    38101 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    38161 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    38221 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    38281 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    38341 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    38401 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    38461 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    38521 ggagtagaat caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    38581 atggccaatg tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta
    38641 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    38701 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc taccatggga
    38761 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    38821 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    38881 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    38941 attacttcta atatgatgca attaagagat atactattag gttgtttgat agtactagta
    39001 ttgccagagc gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    39061 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    39121 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    39181 gtgctagacc aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa
    39241 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    39301 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    39361 aattttagaa aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac
    39421 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    39481 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc
    39541 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    39601 ataacagttg ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata
    39661 gaaaagaaaa cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    39721 gacctagtag gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    39781 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    39841 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    39901 aaatacatga tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct
    39961 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    40021 attgatacta taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg
    40081 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    40141 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct
    40201 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    40261 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    40321 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    40381 ttaatattgg atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt
    40441 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    40501 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    40561 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    40621 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    40681 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    40741 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat
    40801 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    40861 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    40921 acagagcaat tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    40981 aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat
    41041 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    41101 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata
    41161 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt
    41221 ataagtactt agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt
    41281 gttcagattt agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    41341 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    41401 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    41461 ttccaagcca agtgtatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    41521 ctcaatatgc gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag
    41581 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    41641 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc
    41701 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    41761 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    41821 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    41881 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    41941 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    42001 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    42061 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt
    42121 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    42181 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    42241 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    42301 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    42361 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    42421 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    42481 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    42541 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    42601 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    42661 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    42721 aatccacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    42781 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    42841 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    42901 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    42961 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    43021 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    43081 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    43141 agagccaaaa gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    43201 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    43261 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    43321 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    43381 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    43441 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    43501 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    43561 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    43621 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    43681 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    43741 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    43801 tgatgtatgg tggcaaaatc aagtcagaaa aaaattaata gaataatagc tgttgggatt
    43861 gtgggaatcc tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa
    43921 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    43981 tcaaaaactt acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa
    44041 atttttataa tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga
    44101 taaatttata aaaacgtgct aagattggac ttgaaataca gtaatatagt aggtaataat
    44161 gttttaaatt ttatttattg accataatat ataaataatg atattattag acttaactgt
    44221 ttaaataaaa cttttctttt tcatgccttc aatttccaaa ataagtatgt atataaatga
    44281 atatatagat gaggaggatt tttttgattt tagatgataa agtattagaa ggattaggta
    44341 gtaagacctt agttgagcta cgagagatag ctaaagaatt aaaaataaaa tcaattacta
    44401 catataagaa aaatgagtta attgagatta taaattccaa aagtaaaaat gaggaaaaag
    44461 taaacgaaga gactaaagaa aaaaatgaag atattaaaga agacataaag aatagaaaag
    44521 aaaatataga gcgtgaaagt atagaaaaag aagttgaata taagagccct accaaatcat
    44581 acaacaagaa agaaatagat acccaaaata tttcccaaaa ccaagaacat agaaatcaaa
    44641 gaaatgacta taataagaca acgagtgtaa atgatagcaa taagtcatca aatatatcta
    44701 atagaatggt cagaaataat aataaaaact attatatgcc taaacaagtt gatgaatcta
    44761 agatagtaga tgaatttaac acatcaaaag aggatgaagt agtaggtgta cttgaaattc
    44821 tgccagatgg atttggtttt ttaagaggtt caaattattt gtctactgaa ggagatgttt
    44881 acgtttcacc atctcaaata agaagattta atatgaaaac aggagacaag attaagggaa
    44941 taactagaca tccaaaaagt ggagagaaat ttagagcact tttatatgtt caaaaaataa
    45001 atgatgaaaa ccctgacaca gctatacaaa gaaatgcgtt tgagacatta acaccaatat
    45061 ttccagaaga acgacttaca ctggaaacta atagaaatga aatagctaca agaattatag
    45121 atttaatttc acctatagga aaaggacaaa gaggattgat agtagcacct ccaaaagctg
    45181 ggaaaacagt tcttttaaaa agcgttgcaa atagcatagc taaaaatcat ccaaatgttg
    45241 aacttatagt actccttata gatgaaagac cagaggaagt tactgatatg aaagaatcta
    45301 ttgaaggaga cgttatatat tccacttttg accaagtatc aagtcatcat gtcaaggttg
    45361 cagagatggt gttaaataga gctcaaagat tagttgaaca tggtaaagat gtagtaattt
    45421 tactagatag tataactaga ttggcaaggg catataacct tacaatatct cctacaggta
    45481 gaactttatc tggaggtatt gaccctggtg cacttcatgg acctaaaaag ttctttggtg
    45541 cagcaaggaa tattagacag ggtggttctt tgacaatatt aggaacagca ttggttgaaa
    45601 ctggttctag gatggatgat gtaatatttg aggaatttaa aggtacagga aatatggagc
    45661 tacatttaga tagaaaatta gcagaaaaaa gaatattccc tgcaatagat atttacaagt
    45721 ctggtacaag aagagatgat ttattgcttg atgatgaaga aaagactgct ctttggagat
    45781 taagaagaga aatgagtaat aattcagtaa tggaaattac agataaagtc attgaactta
    45841 taaagagaac taaggacaat aaagagtttg taaaatcaat aaagaatttg taaaatcaat
    45901 aatacaaatg caaatatgga ttatataaac taatagaaaa atcacttgaa tggcataata
    45961 agttgtgcta taataatgta gttgataatg taaaaagaaa gttaatataa ttaatttttt
    46021 taaataaaga gaagaggtga ctataatgca aaaggaaata caaccaaaat acaatccagt
    46081 tgaagtgcgt tgtgcttgtg ggaacacatt tgttgctggt tcaactaagg acgaaataaa
    46141 agttgaaata tgttcagagt gccatccatt ctatacagga aaacaaaaga acatagaaaa
    46201 aggtggaaga atcgacaaat tcaagaaaag attcaaaatg gactaatttt tataaaagtg
    46261 gagttggagg aagccaactc ctattttatt acaaaaaaac attcatattc tactttaaat
    46321 tgtctttttg tattataata ataaatatgt aaaattgagt attctaaata caataaaatg
    46381 aggtgagtgg atggcaaaac aagctgtagg tgggcaagct gttattgaag gtgtaatgat
    46441 gcagtcaaaa gacaaaagag cggtagctgt tagaaaaagt gacggagaaa tagcactaaa
    46501 agaagataga ataaaaagtt gggtaagaga taagaatata gataagatac cttttgttag
    46561 aggttctttt gttatgatag atacaatgat tcaaggcata aaaagtttaa acttttcttc
    46621 agagtttttt atggaagaag ctgaagagga taaatttgat ttatttataa agaaaatttt
    46681 taaagataag gcaaatgata taattataat attttcattg gtcatagcaa tgttactttc
    46741 tgctggatta ttcattttta tacctacatt ggtaggagga gctttttcta aagtaatgcc
    46801 taatgatttt atgcttaatt taatagaagg aatcataagg atagctatat tatttgcata
    46861 tatagtattg atttcacgaa gtaaggatat agaaagagtc tttcaatatc atggagcaga
    46921 acataaatct atatattgct atgaaaatga tttagaactt acagtagaga atgctagaaa
    46981 atttaaaaga ttacatccta gatgtggtac aaatttccta tttatagtta tggctgtatc
    47041 aataatattg tttgcatttt ttggttggcc aaatccaatt cttagaatat ttatgagaat
    47101 tatatgtgtg cctatagtag caggtttatc ttatgaggta attagagttc ttgggaaata
    47161 tgataatgga tttactaaaa ttattgcata tccaggaatg atgttgcaat attttacaac
    47221 aaaagagcct gatgatgagc aattagaggt tgctttggaa gcattgaaag cagtagtaga
    47281 ttagagaaaa ggatatttgt tatgacgata aaagatataa ttattaaata ttcagataaa
    47341 ctaaaagata taagtgatac tccaagatta gatacagagt tattgttaca aaaaacattg
    47401 ggtgttgata gattatatat tcacttaaat ctgaataaag agctaactga agaacaaaaa
    47461 acaaaattta tgggttttgc agaagaaaga ttaaatggaa gaccaatagc atatatagtt
    47521 gaaaataggg agtttatggg attagatttt tttgttaagg aaggcgtatt gattccaaga
    47581 ccagatacag aaactttagt agaagaaata attgaaatat gtagagaaaa aaaagatgta
    47641 agtatattag atataggaac tggttctgga gctattacaa taagtttagc aaagtacatt
    47701 gaaaattcta aaatcatgtc ttttgatata tcagagacag cattagaaat agctaaaaaa
    47761 aatgctatta taaatgaagt tggtgaaaaa ataaagtata ttaattctga tttatttaca
    47821 gcaataagtg atagtaacat aaaattcgat atcatagttt caaatccacc atatattaaa
    47881 aaacaagata tagaaactct gcacaaacaa gttaaagatt atgaaccata taatgctctt
    47941 gaaggtggag aagatggatt agacttttat agaagaataa cagaacaagg taagaaatat
    48001 ttaaataagt gtggaatatt agcatatgaa gttggtcata atcaagctga agatgtaata
    48061 aatattatga aaagtaatgg atataaaaaa atatacacaa agaaggatat tcaaggcatt
    48121 gatagagttg ttataggtta caatatatga tataatcaac aattgatatg ttacatttaa
    48181 tcgaggtgaa agtatgttaa aaaaattaga ggtattggaa gatacatata aagacttaag
    48241 tgaaaaaata ggtgatccgg atgtaataaa tgatcaaaaa gtttggcaga aatatataaa
    48301 agaacatgct gacttggaac ctattgttat gaaatacaga gagtataaaa gcgttttaga
    48361 tagtataaaa gaatcaaaag aaattttaca agaagagtca gatgaagaat taagagaatt
    48421 agctaaaatg gagttagctg agatggaaga aaaagtagca cctctggagg aagaaataaa
    48481 aatactatta ttgcctaaag accctaatga tgataagaac gttatagttg aaattagagg
    48541 tggagctgga ggagatgaag cagctctatt tgctggtgat ttatttagaa tgtactcaag
    48601 gtatgctgaa agaagaagat ggaaaataga attattgagt gcaagtgata ctggagttgg
    48661 tggatataaa gaagtatctt ttatgataaa aggtaaaggt gcttattcaa gattaaagta
    48721 tgaatctgga gttcatagag ttcaaagaat accatcaact gaatctggtg gaagaataca
    48781 cacatcaact tctactgttg cagtattacc agaagttgaa gatgtagagg ttgaaataaa
    48841 tccaaatgat ttaagaatag atgttttccg ttcttcgggt aatggaggac aaagtgtcaa
    48901 tactactgac tctgctgtta gggtaacaca tatacctaca ggtgaagtag tatcttgtca
    48961 agatggaaaa tcacaattaa aaaataaaga gcaagcttta aaaatattaa aagctagact
    49021 ttacgataaa gcattggcag aacaacacaa agatatagca gcagaaagaa aaagccaagt
    49081 cggaacagga gacaggtcag aaagaataag aacatataat ttccctcaag gtagaataag
    49141 tgaccataga ataaatttaa ctttatataa gttagatgca ttcttagatg gagatataga
    49201 tgaaatgata gatgctttaa ttactgttga ccaaacagaa aaaatgactg caatataagt
    49261 aatgttttaa agaagctgtt tcttcctaga aattattttc tattttgaaa cagctatttt
    49321 gcattttagg tcatgttatc atattctatt gtatataatt gttatataca agtataggag
    49381 ggttatatga tactaaaagt aacaataatt gggcttttgg caggtgttat agggacagga
    49441 ttaggaggag taatatcagc catttttaaa agagaggtcg ataaatatct aagttttttt
    49501 atgggattat ctggtggaat aatgttggct gtagtggtgt ttgatttaat gaaagaatcc
    49561 atggacaaaa tggggataat caatacagtt atatttacgt ttgttggagc tctcataact
    49621 atgtacataa aaacaaaatt agatgtgtct ggaaatatgg catctggata tcttatattc
    49681 ataagtatat tactacataa tttaccagaa ggactagcca ttggttcttc ttttatgtca
    49741 actgaaagtt taggtattac acttgctata gttataggtc tacataatat accagaaggg
    49801 ttagctatgg cattaggctt agtttgcaat aaaatgaaac taagtaaagt tattttgttt
    49861 actgtaatag ctggattacc tatgggtcta ggaagttttt taggtgttta ttttggagga
    49921 gtatttactt ctcttatagg agtatttctt gctacagcag gaggtactat gatgtatgtt
    49981 gtattggaag aaatttttcc acattcaaaa agtgtgtatt gtataattgg tttcttgtta
    50041 ggtacaatga ttgtaaatta tatataaaat taaaatgaaa gcaggcatac aaaagatgaa
    50101 aactattata agtaatattg atataaataa tattgattat gaggaaataa aaatacaagc
    50161 aaaacttctt agagaaggta aaacagttat ttttccaact gagacagtat atgggttggg
    50221 tgcaaatgca ttagatgaaa atgcagtaaa gaagatttat gaagctaaag gaagacctag
    50281 tgacaatcca ttgatagtcc atatatatga aaaagaagaa gtatatgact tagccaaaga
    50341 tataagtgat aaagctaaac ttgttataga aaagctttgg ccaggaccta taacaattat
    50401 attaaataaa aaagatataa ttccatacaa aactagtgga gggcttgata cagttgcaat
    50461 aaggatgcct tctaatgtta ttgccagggc aataataaaa gaagctggta tacctattgc
    50521 agctccttct gcaaatatct caggtcgccc atctccaaca aaagcaaaac atgtatatga
    50581 agagatgaat ggaagagtgg atggaatagt tttaggagga gacagtaatt ttggattgga
    50641 gtcaactgta ttggatttaa cagaagagac tcctatgata ctgagaccag gaagtataac
    50701 taaagaagtt ttagaggaac ttcttggaga agtaaagctt gacccatcat taagtaaaaa
    50761 agaagataat caaaaagcta aagctcctgg aatgaaatat aaacattatt ctccaaatgc
    50821 agatgtatat ataatatctg gaaaaagaga aaatgtagca acaaagatta atgatatgat
    50881 aaaatctaat agagacaaag gtctaaagac aggtgtaatg tgtattagta gaaaccgaga
    50941 gttttatgat ggagaagtaa tcgatttagg aaactctctt gaagaagttg caagtaatct
    51001 gtttgatgct cttatagaaa tggacaaaaa gggtgttgat gtcatatatt cagaagagtt
    51061 ccctaagact ggagttggac aagcaattat gaatagacta ctgaagtcag caggatacaa
    51121 agtgataaag gcttaagagg gagttgtaaa atatgaatat acttattgtg tgtacaggta
    51181 atacttgtag aagtcctatg gctgaggcta tactaaggaa agcaattaaa gaatctggaa
    51241 gaagtattga agaatattct atatcatcag caggtatatc tacagcaaat ggaatgggtg
    51301 cttctgaaaa ttctatagaa gtactaaaag aaatcggtat agacttatca aatcatagaa
    51361 gtaaagtaat tactaaaaaa ttaattgatg aatcagatat aatactaaca atgactaaat
    51421 ctcataaaga aattttagta caagcagttc caaaatgcaa agaaaaagtg tatactttta
    51481 aagggtttgc aaataaaaat gaggaagata tatctgaccc atttggtgga aatttggata
    51541 tttatagaag cactatgaga gaaataatgt attctgtcaa cgaaatagta aaaaaaatat
    51601 gaaacggagg ataaacaaat gaagatagga ttaggctgtg accatggagg atataattta
    51661 aaaaaagaaa taataagcta tttagaaagt aaaggtatag aatgtgtaga ttacggaact
    51721 aataatgcta cagattctgt tgattatcct gtttatggag aaatagttgc aaattcagtt
    51781 ataaataaag aagtagatta tggaatatta tgttgtggta cagggatagg aatatcttta
    51841 gctgctaata aggttcctgg gataagatgt gcagttgtat cagatgtatt ttctgcaaaa
    51901 atgtcaaaag cacataatga tgcaaatatg ttatcattag gcgaaagagt tttaggaaaa
    51961 ggtctagcac ttgagatagt tgaagcatgg attaatacgg attttgaagg tgatagacat
    52021 gctagaagag taaacatgat taaatcaata gaagaaaaac ataataaata ataaaaatta
    52081 cagttttatc aggaggaatt atcaaaatga gcaaagtggt agagacaaac catccattaa
    52141 tacaacataa attaacttta atgagagata aaaatactgg ttctaaagac tttagagagc
    52201 ttttaactga aattgcaatg ttaatggggt atgaaataac aaaagatata ccattaaaag
    52261 atgtagaaat agaaactcct attcaaaaaa catcatctaa agtagttgca ggtaaaaaat
    52321 tagcaataat tccaatttta agagcaggtc ttggaatggt agatggatta gtgagcttaa
    52381 tgccagcagc aaaagttgga catgtaggac tatatagaga cccagaaaca ttaaaaccag
    52441 ttgaatatta ttgtaagctt cctcaagaca taggagaaag agatataata gtagttgacc
    52501 ctatgcttgc aacagggggt tctgctgtag cagctataga tttacttaag tctaaaggag
    52561 ctaaaagtat aaaattagct aatttagtag ctgcaccaga aggtatagca gaagtgcaaa
    52621 aatatcatga tgatgttgat atatatgttg catcagttga cgaaagatta aatgaacatg
    52681 gatatataat tcctggtctt ggtgatgctg gagatagatt gtttggaact aaataaaaat
    52741 aaataactat aaatatttat tagggacttt cttatatagt gtatataatt ttatgtaaga
    52801 aggttctttt ttatatattt tttctcaaaa taggaattat tgaattgata aatttctcga
    52861 attgagaata aaaagttttt accaattaaa aaagattgct tattttacaa ggttttatag
    52921 aatagtagtc tattttaatg tggcatgtta gttgcttacg attaagatta taggtattaa
    52981 gtgtattagt taaattgaga attgaatatt aataatatta ttgggagtgg taatacatga
    53041 agaattgtat agtagtaaga ggggctggag atttagcaac agcagttatt cataaattac
    53101 acaacagtgg ttttaaagta gttgcgttag aaatagaaga gccattagct ataagaaggc
    53161 aaatttcttt ttgtgaggct gtttatgaaa aaaaagtaac tgttgaaggt gtagaatgta
    53221 tattttgcaa tactgaaaaa gaaatagaag aagttttatc tcaacataaa gtagccttga
    53281 ttatagaccc aaaaggagaa aaattatcta aattaaatcc aatcgttttg gttgatggaa
    53341 tactcgctaa gaaaaactta ggtacaacta aagatatggc accacttaca atagctttag
    53401 gtcctggatt ttgtgcagga gaagatgtag atgttgttat agagacaatg agaggtcata
    53461 atctaggaag aataataact aatggtagag cagaaaaaaa tacaggagta ccaggagcaa
    53521 taaaaggttt ttctaaagag agagttatgc attcacagta tgatggcata atggaaaatg
    53581 tatgtaagat aggtgatata gttgaaaagg gagacctact ttgctatatt aataattatg
    53641 aaaaaaaaca tgaagttaga gcaacaatat ctggagtcct tagaggttta ttaaaggatg
    53701 gagcaaaggt aaacaaaaat ttaaaaatac tagacataga ccctagaaaa gaagaagtta
    53761 agaatgcttt tacaatatca gataaagcta gatgcatagc tggaggggtt ttagaagcca
    53821 tactaaacaa tgggatttat ccccaaaatt aatttatatt agataaagct attaatattt
    53881 tattaagatg atataataaa tttaatagat taaagagagc atatgacggg tatttaaaat
    53941 ttaaatggta aaaatgctct ttttttattg gacattcaat taacatgtgt atataatata
    54001 aaaataagta tttatttagg ttgaaatcta ataaatatat aataccagta cataagcaaa
    54061 gaggagaaaa gtaaattatg agaccatcat gggatgaata cttcatggaa attgcagaag
    54121 tagtgaaaaa gcgttcaact tgtattagaa gacaagtagg agctgtaatt gtaagagaca
    54181 aacaaatatt aacaactgga tataatggtt ctccgagaaa tcttgaacac tgtgaaaata
    54241 ttggatgtaa gaggcaggaa ttaaatattc cctcaggaga aaggcatgaa ttatgcagag
    54301 cattacatgc tgaacaaaat gcgataatac aagctgctca taatggtatt agtgtagatg
    54361 gggcaacttt gtatgttact acaagacctt gtgttttgtg tgcaaaaatg tgcataaatg
    54421 caggtatatt gaaaattgtt tatgaaggtg attatccaga tgatatgtct acagaattat
    54481 taaaagaagc aggaatagaa ctcataaaat tttagtggat aaaatttata agacaataaa
    54541 tgtcaaatta tgacactgaa atacaatggt atcaaatgat taaaagaaag tatttgaaaa
    54601 atcatcagat tagtagtaag atatatttta taattaaata aatttatgag atagattaat
    54661 tacatgttat ttattttata ataatgaggg atactattta tgagtaaaag aaatacacat
    54721 acagaaattg caaagttgtt tagtttaata agtcaaatag gattgatgat tgtaatatca
    54781 atattaggat gtactttctt aggaaaattt ttagatagta aattgaatac tactccagtt
    54841 ctgacaataa tatttttatt gttgggtgtt ggaggtgctt ttagtggtgt ttataaaact
    54901 ctaatagtat atacacgaag gaagtgatat attggatata aaattactta accaggtaag
    54961 gtcagtaaca aaaggtatag taatatttga tgtagtttta attgtgctaa tgttgataac
    55021 atcaacatta agtaaaccag tattattagg acttatattt ggctctataa tagctatgtt
    55081 aaattttaga ttattggcga tatcactgga aaaatcagtg acatttcctg ctgggaaggc
    55141 acaagcttat gcatcagcac aatatgctgt tagattattt ataatggcag ttgttctaat
    55201 tgcatcagct acagtaccac atttaaatat aatcggaaca gcattgggat tattgagcac
    55261 aaagtttgtc atattatcaa aaaactttat aaataaatta aagaggaagg aggcataagt
    55321 atgaatcaag cgcattatta tctcatagga aactttaaga ttagtgaaac aatactagta
    55381 agttggggaa taatggcctt tttagcgata gcatcttatt ttttgactag aaacttaaaa
    55441 aaagttccaa ctagtaaagt tcaaatattc ttagaatttg cagtaggtgg tcttgctaaa
    55501 atggttacag atgttatggg taaaaataca gtaaaaagaa tgccatatat gataccatac
    55561 ataggaagct tgtttttatt tttcgcatgt tcaaatttaa gcccattact tggattaagg
    55621 tcaccaacaa ccgacttaga cacaactgct gcatgggcaa tgattacatt ttttatgatt
    55681 tattttgcag gagttaaatt taaggggtta tcatacttta aagaattggc tcaaccactt
    55741 gccataatgt taccaatcaa tattataggt gagttagcta gaccaatatc attaagtttc
    55801 cgtccatttg ggaatatttt aggtggaaca ataataatgg gtctgtttta tcaactttta
    55861 ggttttatat ctagtttaat accaggatta tcaataccaa ttggagaaat attaatacca
    55921 gtcccattac acttgtactt tgacttattt gcaggattgt tacaagcata tatatttata
    55981 atgttaacaa tggtatttat atcaagtgca ggagagttag attaagaaat caacttatat
    56041 taagataaaa ataaaataat ttaaaaaatt aaagttttaa ggaggaaata attatggaaa
    56101 cagcaatagt agcggcagca tcagcaatag gagcagggat agcagttgca acaggtatag
    56161 gtgcaggtat aggtcaaggt atagcagcag ctaaagctgc agaagcagta ggaaaccagc
    56221 cagaagctaa aggtgatata acatcaactt tattactagg agttgctata gcagagtctt
    56281 cagcaatata tggtttggtt atatcaataa tacttctatt cgtaaatcca ttctttaaat
    56341 acttaggaat gtagatttag tccttaaata ttttgagata ggtttatagg taaatcatct
    56401 caaacttata atcatgagat gaactttatc atctcgtgat tataagtagt tatgatttat
    56461 ggaaaggagg ataattatgg aaaaagcact agtaggtata acgtgggaat ttgtgttcca
    56521 aattgttaat acttttataa tatttttact tctgagaaag cttttattca agccagtatt
    56581 aaatataatt gaatcaagag aaaatgatat taaaagtgat ttggcagaag gcgaaaaagc
    56641 aaaaaatgaa ggtttagctc tgaaaaaaga atatgagtca aaaatcaatt ttgccaaaga
    56701 tgaaggtcaa gaaatcataa aacaagcaac tataagagca gagcagaaat cagatgatat
    56761 agtaaatact gcaaagaaag atgctcttga tataaaagaa aaagcaaaca aagatataga
    56821 acaagaaaga caaaaggtta taaatgaaat caaaaatgat atatctaaca tagcactact
    56881 tgcagcatct aaagttattg aaaaagattt agataaatct aaacatgaag aattgataga
    56941 aaactttata aaagaggtag gggaagctaa atgataaatg taatagcaaa tagatatgcc
    57001 gaagccctat ttcaattagg agaagaagaa aattcaactg atgtcttatt caaagaacta
    57061 gaaaaagtag tagatatgat gactaaagtt agtaaagatt tctacaaagt tttaaaatca
    57121 ccattagttt caaaatcaga aaagaaaaac ttggtagaaa ttatttttag taaggaagta
    57181 agttctaaca taaaaaactt tttaaaagtt ttagtagata aagatagaat atcatattta
    57241 gaagatatcg aattagctta taaagaatta ttaaataaga aaaacaatgt aattgatgga
    57301 gtagcaataa gtgctattcc aatgagtgaa acagatatta aagaacttga agtcaaactt
    57361 tcaaataaat acaacaaaaa tgttactata gaaaatgttg ttgacaagac tatactaggg
    57421 ggagttctag ttagaattgg aaatgaacaa atagatggca ctgtgaaaac tcgtcttgat
    57481 aagatgaaag aaaagctatc tgaagtaata tcttaagaat ggcggtgaac ccatgaactt
    57541 aaaacctgaa gaaataagtt ctataattaa acagcaaata aaaaattatg agaataaagt
    57601 tgagttgaca gatacaggta gtgttttaac agttggggat ggtatagcga gtgtatatgg
    57661 attagaaaaa gctatgtctg gtgagttgct agagttccca ggtgaaatat atggtatggc
    57721 acttaacttg gaagaagaag tagttggagc tgttatcctt ggagatgaca gtgaaataaa
    57781 agaaggcgat atagttaaga gaactggaag aatagttgaa gttccagttg gagaagcttt
    57841 aataggtaga gttgtaaact cattaggtca gccaatagat ggaaaaggac ctatagcgta
    57901 cacaaaaact agaccagttg aatctgaggc acctggtata atagatagaa ggtctgttta
    57961 tgaaccatta caaacaggaa tcaaatcaat agattcaatg ataccaattg gtagaggtca
    58021 aagagagtta ataataggtg acagacaaac tggtaaaaca tctatagtta ttgacactat
    58081 actaaatcaa aaaggaaaag atgttatctg tatatatgta gcaattggac aaaaacgttc
    58141 tactatagct caattagtta gctctttaga aaaaggtgga gctttggatt atacaatagt
    58201 agtttcagca acagcatctg aatcagcacc acttcaatat atagcaccat atgctggagc
    58261 agctatgggt gaagaattta tgtacaatgg gaaacatgta cttatagtgt atgatgattt
    58321 aagtaaacaa gctgttgcat atagagaaat gtcattgtta cttagaagac caccaggacg
    58381 tgaggcatat cctggagatg tattctattt acattcaaga ttacttgaaa gagcagctaa
    58441 gttatctgat gaattgggtg gaggttcaat gactgccttg cctataatag aaactcaagc
    58501 tggtgacgtt tctgcatata taccaacaaa tgttatatct ataacagatg gtcaaatata
    58561 cttacaacca gagttattct attcaggtgt aagaccagca gttgaccctg gtatatcagt
    58621 atcaagggtt ggtggttctg cgcaaattaa agcaatgaaa aaagttgcag gaacattaaa
    58681 acttgcatat tcacaatata gagaacttgc agcattctca cagtttggtt ctgacttaga
    58741 tgaagatact aaaaagagac ttgctcaagg tgaaagaatc gttgagatat taaaacaagg
    58801 tgagcatcaa ccaataaaag ttgaaaatca agttatgata atatatgctg ttataaataa
    58861 tcatttagag gatattccga tagataatat agctagattt gaatcagaat tgtatgcatt
    58921 tgtagataat aattatccgg aaatatcaag aaaaatatta ggtggagaag acttcactca
    58981 tgatttaaca gatgcaataa atgaatttaa agaaaagttt gttgttgaag tataatcctt
    59041 tttaaaaaag gaggtaaatt aattggcagg agctggaatc aaagcaatca aaacacgtat
    59101 gggaactgtt gaaagtacaa agcaaataac taaagcaatg gaattagttg catcttctaa
    59161 gcttagaaaa gcaaaagaaa aggctgaaga ttcaagagaa tattttaata ctttatacaa
    59221 tacagtaaaa gatattgccc aaacaacacg tggaattaga aatgaatttt taaaagaaag
    59281 agaagtaaaa aataaatgct atgtagtagt agctggagac agaggacttg caggtggata
    59341 caattctaat gtattaaagg aatctgtctc tcatatgaaa ggtaaaaagg aaacagtgat
    59401 tactgttgga aagaaagcac atgaattttt ttctaaaaga aactacaata tagcaaaaga
    59461 tataaatagt gttgaagctt gtggatatga agatgcgaca gaaatttcac aaactattat
    59521 tgacctgtat aaaaaaggtg aagtagatga agtttatatt gcttatacaa aatttaaatc
    59581 tgctttatca caagaagttg aaattttgaa attgcttcca ttagtctttg aaaaagatac
    59641 agacgaacaa ggaatggcag taaaagaaga aaaacaaaat agatcaaaag tacaatatat
    59701 accatcagca gaagcagttt taagttatat tgtacctcag tttatatcag gtacagtata
    59761 tggtggaatt ttggaatctt ttgcatcaga gcaaggtgct agaagaactg caatggaatc
    59821 agccacagac aatgcgaatg aaatgctatc taatttagag ttaagttata acagagcaag
    59881 acagtcagct gtaactcaag aaataacaga gatagttggt ggagttgaag ctctaaaata
    59941 gggaggttag gaaatggcaa atgtaggtaa agtagtacag attgtaggag ctgtactaga
    60001 tgttaagttt gatggtgaac aaagcctacc taacttatta aatgcgttag taataaagtt
    60061 aggagataaa gaaatagttg cagaagttgc tcaacatata ggtgatgata ctgtaagatg
    60121 tatagctatg agtgcaacag acggacttgt aagaggtatg gaagttgtag atacaggagg
    60181 gccaataagt gttcctgttg gagatgagac tttaggtaga atattcaatg ttcttggaaa
    60241 accagttgat gggaaaccag ctccaaaatc tgcacctaaa ttacctatac atagaccagc
    60301 accggcgtat gatgaactag aaactacagc agagatacta gaaactggta ttaaggtagt
    60361 tgacttacta gctccatatc taaaaggtgg aaaaataggt ctatttggag gagcaggagt
    60421 aggaaagaca gttctaatac aagagcttat aaacaatatt gctaagcaac atggtggtat
    60481 ttctgtattt tcaggagtag gagaaagaac aagagaaggt aacgaccttt atggcgaaat
    60541 gagtgagtct ggagttataa ataaaacagc tctagtattt ggtcaaatga atgaaccacc
    60601 tggagcgaga atgagagttg ctttaactgg acttacaatg gcagaacatt ttagagatga
    60661 gcaaggacaa gacgttttac ttttcgttga taatatattc cgtttcacac aagctggttc
    60721 agaagtttca gcacttctag gacgtatgcc atcagctgtt ggttatcagc caacattagc
    60781 tactgaaatg ggtgcacttc aagagagaat aacatcaact aagaaaggtt caataacatc
    60841 tgttcaagca gtatatgtac cagcagatga cttaactgac ccagcaccag ctacaacatt
    60901 ctctcactta gatgcaaaaa cagttttatc aagacaaata tcatcactgg gtatatatcc
    60961 agcagttgac cctcttgaat ctacatcaag gattcttgac cctagtatag taggaaaaga
    61021 acattatgaa gtagctagag gtgtacaatc aatacttcaa agatataaag aacttcaaga
    61081 tataattgct atacttggta tggatgaatt atctgatgaa gataagttaa tagttgcacg
    61141 tgctagaaag atacaaagat tcttatctca atcattcaca gttgctgagc aatttacagg
    61201 aaatccaggt caatatgtac ctgttaaaga gactgtaaga ggatttaaag aaattcttga
    61261 agggaaacat gatgacttac cagagtcagc attcttattt gtaggaacaa tagaagacgc
    61321 tgttagaaaa gcgaagggaa gtatgtagtc atggccagtg aattttcatt gaaagttgta
    61381 actccaagta atacttttta tgatggagat gttgaaatga tagtagttag aactacaaaa
    61441 ggagacaaag gggtgttgaa aggtcatata ccatttgttt caggtatacc tgtaggaact
    61501 ttaaagataa aaaaagaggg aagttttaaa gaggcgaaaa tagcagaagg attcattaat
    61561 gtaactcaag ataaaactgt catattaaca gaatctgctg agtggattta ataaattaat
    61621 ttagagtata aaaagtcatt ttgaccaaaa aaacttttgg gtaaaatggc ttttttgtgt
    61681 tttggtgtta ataaaataac gataaaattg taagttttta gtgataataa attcacaaaa
    61741 tgtttaaatt tactgccaaa ttatgttgta attaataaga taaagaagat ttagatagct
    61801 tttatgtaag tgaaaaagtc ttcatataag aaatacatat aaaattttta taaaatttaa
    61861 aaaatttgct tagttgtgta gtataataaa taatataata gatttgtata taatattcat
    61921 tacaaataat tttaaaagtt acagttttat tataatgatt attaaattaa gataagtaac
    61981 ataaactaaa taataatgtg tattatcgat agtaaattta taaattcatt attaattatt
    62041 gaaagaagta ttataaattt tctggaggaa caatgaatat atttgatatt ggcgtagata
    62101 taattgaaat tgatagaata agaaaggctg tagacaaaaa taataggttt ttggagaaga
    62161 tatttacaga tagagaaata gagtatttta attctaagaa ttttaaagca gaaagtatag
    62221 caggtaattt cgcagccaag gaagctataa gtaaatctat aggtacagga attagattgt
    62281 ttaattttaa agatatagaa gttctcagaa atgaaatggg aaaaccaata gtaaaaactt
    62341 ataataatct agcaaaaatg tgcatagatt ataatgtttt agaaataaaa gtttcaatat
    62401 cacattcaaa agattatgct atagcaaatg cgataaccat aattaaggac taaggagtga
    62461 ttacattatg atggataaaa cagcaaaaga aataatgaca acagatgtaa tagtagcaaa
    62521 acaagatgat agtatagcag atgtagctaa tatgctaatt gcagaaaaaa taggaggtct
    62581 gccagttgta gattcagaga atagagttgt tggaataatt tctgaaacag atattcttaa
    62641 aaaagaaaaa tatatagaag cacctctcta tataaatcta ttacaaggat tgattttctt
    62701 agatgactta aaaaaagttg aaaaagatat aaaacaagta gctgcttata aggttggaga
    62761 acttatgtct aaagatataa taaaggttca tgaagatgat aagtttgatg atgtagctaa
    62821 tgtaatgatt aaaaaatcaa taaacagagt tcctgtagta gatgatgaca acaaacttaa
    62881 aggtataatt tgtagatatg atataataaa agctttatac aatgaataaa ctgaagtagg
    62941 aggtgtgatg tgagaaaaaa gtggaccata gtatgtatta tgttcttggc actggttata
    63001 attgtaatag gatgccaaaa acgacagtcc acaaaagaag aagtatacaa agattttcaa
    63061 aagcagattt ctgatatgaa ttattacagt tgtaaagctg aagtagaagt agttggaaat
    63121 aaaagtccac acaattatgt tttaattcat acttataaaa aaaccgataa ctataaatta
    63181 gaagtaattt ccccaaaaca tttaaaaggg aaaagtatag aatatcaagg tgataaaata
    63241 ttagttaaaa atccaaagat tagtgatgta gtggagttac ctaatacagg aaaaaataat
    63301 caatatcttt ttgtaggaga ctttataaaa aactatcttc aaaatgagga gatgaaagta
    63361 aagttatcca aaggacattt ggttttagag acttttattc ctggggataa taaatatttt
    63421 aacaagcaag tattatatgt aaatgctgac acaaaaaatc ctgaaaaaat ggaagtgtta
    63481 gataaagagg gagtgccaag atttacagta aaatacaaag attttgaata cagaaactaa
    63541 gggggactaa agacatgcaa aaaataacag tgcctacatg ggcagagata aatctagata
    63601 acttaagatt taacttaaat aatattaaaa atttattaga agaagatatt aagatttgtg
    63661 gagtaataaa agctgatgca tatggacatg gtgcagtaga agttgcaaaa ttgctagaaa
    63721 aagaaaaagt agattactta gcagtagcaa gaactgctga aggaattgaa cttagacaaa
    63781 atggcataac acttcctatt ttgaacttgg gatatactcc agacgaagct tttgaagatt
    63841 ctataaaaaa taaaataact atgacagttt attctttaga aacagcacaa aagataaatg
    63901 aaattgcaaa atctttagga gaaaaagcct gtgttcatgt taaaatagac tcagggatga
    63961 ctagaatagg tttccaacct aatgaggagt cagtacagga aataatagaa ttaaataaat
    64021 tagaatatat agatttagaa ggtatgttta ctcattttgc tacagctgat gaagtaagta
    64081 aagagtacac ttataaacaa gctaataatt ataaatttat gtctgataaa ttagatgagg
    64141 ctggtgtaaa aatagctata aaacatgtat caaacagtgc agctattatg gattgccctg
    64201 atttaagatt aaatatggta agagcaggaa taatattata tggtcattat ccatctgatg
    64261 atgtatttaa agatagatta gaattaagac cagccatgaa attaaaatca aaaatcggac
    64321 atataaaaca agttgaacca ggtgtaggaa taagttatgg actaaaatac acaactacag
    64381 gtaaagaaac aatagctaca gttccaatag gatacgcaga tggatttact agaatccaaa
    64441 aaaatccaaa ggttcttatt aagggagaag tgtttgatgt agttggtaga atatgtatgg
    64501 atcaaataat ggttagaatt gacaaagata tagacataaa agttggagat gaggttatac
    64561 tatttggaga aggcgaagtt acagctgagc gtatagctaa agacttagga actataaact
    64621 atgaagtgtt atgtatgata tcaagaagag ttgaccgtgt ttatatggaa aataatgagc
    64681 ttgtacaaat aaacagttat ttgctaaaat aaaaccatga tattataatc tgggaggcga
    64741 ttttgtgtac gataagagta aagaaaaaat agaagttact ttacctgact ctctgatttc
    64801 tgaagttgat agtatagttc agatggagaa cagtaacaga gaagattttg caaaagctgc
    64861 ttttcagttc tatataactc agaaaaagaa aattcatatt aaagaatcta tgaaaacggg
    64921 ttatagagaa atgggtcaaa ttaatctgtc actagctgaa ttaggtatga cagttgaaga
    64981 agtatcttct gatgataaaa atgaagggaa gattgcccaa ggtgagtact aattgtgaaa
    65041 taaagcgtgg tgatttatat tacgctgatt taagtccagt tgttggttca gagcagggag
    65101 gcgttagacc tgttctgata atacaaaatg atgtaggtaa taaatatagt cctacagtta
    65161 tagtatcagc tataacatct cagattaata aagccaaatt acctactcat gtagagataa
    65221 gttccaatga atatgggctt aataaagact cagtaattct tttagagcaa attagaacta
    65281 tcgataaaaa gagactgagg gaaaaaattg gttgtctaga tgaaaatatg atgataaaag
    65341 tagatgatgg acttcaaata agtttaggat tgtttacttt ttaaagaggg atttgtcttt
    65401 tattagacaa tccctttttt cttttaaata tctcttaagg tttaatttta atgtactatg
    65461 ttaataaaat taaaaataac gcaaaaaaat agcataaaag tgatataata atatggaatt
    65521 gatataaaat tacgaactca agggaggtaa ttgcatgaga gaccataaag gattaaatga
    65581 gattgcgcgt atcataagaa gagatatagt ttcaatgata catagagcca aatctggaca
    65641 tccaggaggg tcattatcag tagttgaaat acttactgcg ttatactttg atgaaatgaa
    65701 tgtggattct tcaaatccta aaatggagga tagagataga tttgtattat caaaaggaca
    65761 tgctgcacca gcattatatg ctacattggc tgaaaaaggt tattttgata aggaagaact
    65821 gaatggatta aggaagatag gaaggatgtt acaaggtcat ccagacatga aaggcacgcc
    65881 aggtgtggaa atatctactg gttctttagg tcaaggtttt tcagttgcat gtggtatggc
    65941 tatggcatca aagttggata atgctccgtg gaatgtgtat actttgcttg gtgatggtga
    66001 ggttcaagaa ggaattgttt gggaagctgc tatgagtgct gcacattata aacttgataa
    66061 tttaattgct tttttagata ataatggact tcaaatagat ggagatatag aaagcgtaat
    66121 gagtttaggt tcaatagttg ataaatttaa agcttttggt tggaatgtaa ttgagataga
    66181 tggacatgat tttgaccaaa tatttgcggc tcttgatata gctaaatcaa cagttaaaaa
    66241 acctacaatg atagttgcaa aaactataaa aggcaaagga atatctttta tggaaaatca
    66301 agcaggatgg catgggacag ctccaagtga tgaggagcta gaaaaagcct tgcttgaatt
    66361 aggaggtgct gacaatgagt aaaatggcga cgagagaagc atatggtaaa gcattagtaa
    66421 aattaggtca aataaatgac aatgttgtag tattagatgc agatttatca aagtctacaa
    66481 aaacacatga tttttacaag tcatttccag atagattttt taatatgggt atagctgaac
    66541 aaaatttaat aggcgctgct tgtggattat caactgctgg taaaatacct tttgcaagta
    66601 cttttgctat gtttgctacg ggaagggctt ttgagattat aagaaattca gtttgttatc
    66661 caaagctaaa tgtaaaaata tgtgctactc atgcaggtct tactgttgga gaggatggag
    66721 catctcatga aagtgtggaa gatattgcta taatgagagc tataccaaat atgacagttc
    66781 ttgttccagc tgatggagtt gaaacagaaa aaattatttt tgaaatagct aagtacaatg
    66841 gacctgttta tgttagatta gggagaagtt cagtaccagt tttatttgat gaagattata
    66901 aatttgaaat aggaaaaggt acagtcttaa gagaaggaaa ggatgtatca attatagctt
    66961 gtggaattat ggtaaatgaa gctctattag cacaagaaaa gttgcaagaa gaaggtataa
    67021 gtgctagagt tataaacatg tcctcgataa aacctataga caaagattta atattagaat
    67081 cagcaaaaga aacgaatgct attgtaactg tagaagaaca tagcataatt gggggattag
    67141 gttctgctgt aagtgaggta gtaggagagt cttgtccaac cattgtaaag aaagttggaa
    67201 ttaaagatac atttggagaa tctggtactc caaatgaatt gttaaaaaaa tatgaattaa
    67261 cttgtgatga cataataaag actgttaaag aagttattat tgcaaaaaga atgtaagaga
    67321 tataaattat tcatgtttgt taataatata atatatttaa aagagaggta taaagtgtta
    67381 tattaattta tatctctctt ttttttatat gatatcaata tatcaaaaat gtaataaaat
    67441 attcaaattt ttacaaaaat agagaaaata ttcaaatata tattgactat tcagaaaaat
    67501 atgactaata tataatttat agagaaacta attttatata taatgactac ttagataaaa
    67561 gaatctaaaa aaaattatga gaggaggagt agggtataaa atcagattat aagttttaag
    67621 ttattatact aaaaagctat aaaacagtta tcatgtattt tatgttaaag gaaattgtag
    67681 gtagtttatc gaattgttta atcagaagaa aaaatttcac atggggaggt aactgagata
    67741 tgtcagaaaa agtaaatgtt aaaacagtca tcagtttcgc aggtgcgtat gtagcaacag
    67801 taatcggttc tggatttgca acaggacagg aaataatgca attcttctct ttttatggtt
    67861 ttgcaggtat tattggtggg ataatttcaa tggtattatt ctcatggatg ggtgcttcag
    67921 ttatgtcaaa aggtaaagag cttcaactta aagaacctat caaaatttat cgtgtttatt
    67981 gtggtaagta tttaggaata ttctttgaat ggtttggacc tttattctta tttggtgttt
    68041 tcgtagtaat gatttcagga gcaggagcaa ctttaactga atactatgga ttaaatccat
    68101 ttgtaggtag agtaggtatg gcagtagtag cattattaac tgtttcacta ggtcttgata
    68161 aattatcaaa gatactaggt ggaatagggc ctataataat aatctttaca cttttagttg
    68221 gtggtataag tttagctaaa aacataggta atataggaga agctgcacaa gttcttagca
    68281 ctgtaaagat agctaaacca gttccaaatg cctatctatc tggtgtaata tatactactt
    68341 ataacacaat tgttgttatg gcattcttaa ctggattagg agcaagtgct gctaacaaaa
    68401 aagaagctat ttgtggtggt atattaggtg gagtagcact tatggctgca gctataatga
    68461 tgcacttagc tattctttct gatataggaa acttatattc aaaagcaata ccatcattat
    68521 tcttagcaga taagatttca ccagtaattg gagttttatt ctcaataatt cttatattag
    68581 gtatatatac aacagctgta cctttattat ggtcagtaac taatagattt gtagatgata
    68641 aacatcctaa gtttaaatta ataactttaa tagcagctat attaggtctt ataggtggct
    68701 tcctaccatt tgataagctg gttggtattt tatatccata tactggatat atgggcgtca
    68761 taatactggt ttgtgttctt tatagacaga ttacaaaaac atctggatat caagaaggaa
    68821 tcagtgacaa ataagtattt tttaatattg taaactcaag agctatatga aaacaatgaa
    68881 aatgttttca tatagctctt tttatttttt agctcgttta ttatgaaata gaaaaaacat
    68941 gttttttagt tacattacat atatttataa ttatgtataa atatatacta atcaataatt
    69001 tagagtctaa gataagttta taaatcatat atttagaata tatgggagga gtgataagat
    69061 tgaatgtaaa gtttaatatt aaaggtataa tttatggagt tatagctctt gtattcgtta
    69121 tagggggatt tatattcata ccaagtatgt ttgtagagaa atctaaaccc atagattata
    69181 cagtattaca aagaaattca ataccagaaa agatattaga tgtgatggat aagtatacaa
    69241 atgaagaaag agcattagcg gctaaaatcg ataacaaaat atacattata gtgactagag
    69301 gaaataataa atatggtata gaaatggaca aaatagaaag tgtggttgag gaaggaaagg
    69361 atgttttgag agtcaagata aagtataaag ataaggaaga ttcttttcca tacatagttg
    69421 ttgaaacaaa tatgagtgaa cttccagata gaatagaatt aaatgcgacg aaataagaag
    69481 gtattttata attaaataac tgcatattgt acaataaagt catggttttt attaatgtaa
    69541 gtaaattgtg tatggttctt attgttgtaa aagccatgac ttaaaatgct tataaagtaa
    69601 gatattaaca gtagtattta ttactacatt aaatggggtc tggaaatact taatattcta
    69661 actatttgga gtgataattc tagtttcagt aactatgtag tctaattgtg catcatgagt
    69721 ttcttttgga atagaatcga atatctggaa gtcaaatgct atgccgatag ttatagcatc
    69781 ttttcttaag ttttctaaaa atctatcata aaatcctcca ccatatccta atctatagca
    69841 atgtatatca tatgctacag ctggtacaat tacaacatct aaagccttaa tatcaattgt
    69901 aggtgatttt tcgttaggtt ctcttattcc atatgctcca attctcaatc cctcttttaa
    69961 atctgttatt tgagtaggga ttaatgtatg attttctttt attgtgatgg gacttgatac
    70021 tattttcttt agagacaaaa gttttttgat taaattatct gtttgaactt cattgttaaa
    70081 atttagataa agcattatat tagttgcatt tcttatatct tccatttgaa gtaatttttc
    70141 agttattaaa tttgaattac taagtacaaa gtttgcactc ttattttttc ttgattcaat
    70201 aacttttttt ctaaattctt ttttcataaa ataattcctc ccttaatatg ttataatgtg
    70261 tacagcaatt gtatttaata taaattttag tataatttat tgtaataaag caatatattt
    70321 gggtaaatgt aataaaaata acagctagtc atataataat aatagtttat gtaaaaggag
    70381 atgtatcttg tgatttcaaa gaagaaagct atttttttag gggtgatttt agtaattata
    70441 acagctatgg ttacatcagc atttcagttg acattgggga ataaagttgt aatatcgaaa
    70501 gaactctatg aagactataa aaaatatgat aagttgttag gactagaatc tattataaaa
    70561 caagattttt ataaaaaagt atcagatact gatttggtaa atggagcagc aaaaggtcta
    70621 tttctaggaa caaatgataa gtattctggt tattatacta aagatgaaat ggagaatcta
    70681 attaatgata gtgaaggttc ttatgttggt gtaggtatgt atataggggc atcaaaagat
    70741 ggtggcttag tagtagttcc catgaaagat tctccagcag agaaagcagg tgtaaaatca
    70801 ggggataaat tagtaaaagt caatggaaaa tctgtctcat ataaaaattc agatgaagct
    70861 gttcgtatga tgaagggtaa aaaaggtaaa acagttgaac tgactatatt aagagaagat
    70921 aaacaattga actttaaagt aaaaacagac cagataattg agaagagtat agaaagtaaa
    70981 gtaattgata atgatttagg atatatagaa attacccaat ttatatcaag tacatatact
    71041 gactttgata aagctttaaa agaattaaaa gcaaaaaata tcaagggatt agtaatagac
    71101 cttagaaata atcctggtgg aatgctagat atatgtaaag aagttgctga tgagttaatt
    71161 ggtgaaggaa ccatagtata tacaaaagat aataagggaa atactgagta cttgaaatct
    71221 gataaagaaa aattgggact tccaatagta gtgttaacta atggagaaag tgcttctgct
    71281 gctgaaatat taacagctgc aatcgttgat aataaagaag gtatttctgt agggactaca
    71341 acttttggta aagggttggt acaatcagtt gtaagattga aggatggaac aggatacaag
    71401 ttaactactg ctcaatattt tacacctaat ggagattata tcaatgaaaa aggtataaag
    71461 cctactattg aagaaaaaga tgaaaataaa cagttagatg tagcaactaa gtggcttaga
    71521 gaacagattg ataaatagga tttaattttt ataaacttat actataaaat aaagaacatt
    71581 ataaaagaga tgtctcaaaa tgaacttttt agttcatgag gcactctttt tgtatgaaat
    71641 caaatgtatt ttcatcattt taacaatgaa aaagaggctt atctctattt ttaggaagcc
    71701 tctataactt tatattgaat ctatttatta actttgtaca tacagatacc atttatataa
    71761 gtttcgtgta tattaaagtc attatctgtt actataaagt ctgcatccat accttgatat
    71821 atagagccac agacattatc aatattacaa cttaaagcag ggatatatgt agccattttt
    71881 attgcatctt ctaaagttga tatgttccaa tcaactatat ttctcacagc cttgtccata
    71941 gttaatacac ttccagctaa actcccacta tttaatcttg caacactatt ttttacattt
    72001 acattaaact taccaagagt atattttcca tcctccatag caccagctct catacaatct
    72061 gtaattaaag ctatgttatt actagactta gcatccatca ttattttaac agctattggg
    72121 cttacatgat gaccatcaca aattaattca gcataagtat cttttagtac catagcagcc
    72181 ccaaccattc caggttctct atgatttaaa ggactcatcc cattatatgt atgtacaaac
    72241 actttagcac ctgaattaac gacttgttta acttgctcaa atgtagctga actatgacca
    72301 agagaaactg atatattatt tttagttgca tatcttgtta attctattga acctttacgt
    72361 tctggagcaa ttgctatttt ccttattaaa tttttcgatt ttttttgcca taattttaat
    72421 atattgatgt cagggtcttt aaaatatttt tcattttgag cgcccttata tttttctgta
    72481 aaaaatggac cttctaaata aatcccttgt atttttgcac cctcaactcg attgaagcta
    72541 ttgcttatat tttctacaga atcacttagt gtatctacag aatcagttaa agttgtaggt
    72601 aggaaagatg ttactccata ttttaataag ccttttgaaa ttgtgtttat tgcttcaaat
    72661 gtattgtcca tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac taagccagga
    72721 gcaatatatt tacctgaaaa atctataaca ttatcagtat tattaggtct ttcttgttga
    72781 aattcaccga atttgccatt aattatacta aggtaacctt tttctttgat tccatgtttc
    72841 ataaaaaatt tatctgcata aataaaactt ttcaaaataa cctccttata attatactat
    72901 cattgtggca ttgacgctta taatatcatt ctgttttctt gaaagctatt taacttacta
    72961 tcttacatgt cttactctat atacaaattt atcgcttcta gcgacactca aagtaaattc
    73021 aataactata ttgtcatcat tatatgtggt acgaagtaat ttcagacaag gtgaatctcc
    73081 tggtacttcc aataacttag cttccttatc agagattatt ccagctgaaa attcttcatc
    73141 agcaacttta ataatctcgc cataatcttc tctaaatatt tcatataaag gtttttgttc
    73201 gattttttct tttgtcaatc ctataaattc tttcatagga agatatgttc tttcaagcat
    73261 cataggaagg tcatcagcaa gtcttaaacg cattattttt atcatttttt caccagtagt
    73321 tatacctaat ttttcagaga aatactttgt actttctact acctcaaatg ataatacctt
    73381 tgtacctggc ttttttcctt gtgttctcat ataatctgta aaactataac tttccattaa
    73441 gttttgagaa ttttgactta gtgcagcaac aaaagttcct tttccatgtc ttttgtatat
    73501 atatcccata tgttccaact cagccatagc tagtctaact gtggttctac ttactccata
    73561 ttcatcacaa atttttcttt cggaaagcat tctatcgttt ggttccatct cattttcaat
    73621 cttagttctt aatatctcaa caagctggtc atatattggt cttttatcat ttctttttat
    73681 cataaaaata tagtcctttc taagtggtta ttaccacaat ttgattatag aatatagaat
    73741 tatttatgtc aatgaataaa gtttaatcaa aattcaataa gtaaggctat taatttatta
    73801 taaaattatg gtacattgac aaaacaagta aaataattta tattcaaatt ggtaataacc
    73861 actttggata tattatatga ggtatttttt attttacaga ttaatttatt gtttagaaaa
    73921 gtaaaaatta ataatgagga gaatctagta tgattacagt aataacattc aacccatcta
    73981 tagatagaat gtatagagtg aataatataa acataggtga ggtgcaaagg gtagtaagta
    74041 agaatgcaac tgctggaggt aaaggattaa acgtagctaa ggtatgtaaa attttaaaag
    74101 aaaatccttt agctatggga tttctaggcg gatttaatgg agaatttata aaaaatgaac
    74161 ttagaaaact agatatacaa aataaattta caaaaataga gcaggaaact agaaattgct
    74221 taaacataat tgaggacaat aaagttagta cagaattttt agaaaaagga ccaatagtag
    74281 aaaaaagcga tttagagaga tttgaaaatg atatagagga agtactaaaa aatactaaaa
    74341 tactggtagc ttctggtagt tattgtgaga atatgccgat agactattat gaaaagatag
    74401 gaaatacatg tagagcaaat aatataaagt ttatattaga tacatcagga gaagcattaa
    74461 aaatagcttt aaaatcaaaa ccatatttaa taaaaccaaa tatagatgaa ataagacatt
    74521 tattaggaat gaatatagaa tcaagagagg aaattatctt gtcaggaaaa aaacttattg
    74581 aaatgggtgc agagaatgta tgtatatctt taggaaaaga tggtatggtg tatttaaacg
    74641 aagataatgt atatgatgta aaagtaccag ttgtaaagtg tataaatact gtaggtagtg
    74701 gagatagcac agtggctgga tttagtgtag gtattttaag gggttataaa atagaaaatt
    74761 tacttaaatt tgcaaatgct tgtggtataa gtaatgcttt aaatatggaa actggatttg
    74821 tagatttggg agaaattgat aaatatcaag atttagttga agtaaaaaag ttaagttaaa
    74881 tacatttaat gggaatataa taaatttaat gttgttgtaa tagattttaa ttttaatata
    74941 aaagatatag tcttatataa aaataggtta gaaaaattca tttttaacct atttttataa
    75001 taaatatcta ttttactgaa tttataattt tctctagtaa tctttgagct ttgatacact
    75061 cttctccatt aatagctggt tttatattat tttctataca atcaataaaa tgatttgata
    75121 tatcctcaaa acccttttgt tttaatatat ttacccatgc accactttgc tttaaagtta
    75181 ggtttccacc ttcttctatt tccagtacat taagattctt aactctttga atcttaccct
    75241 ttgaaagaat ttcaacttgt tctaactttg ttccagaatc tctatgcata gaggtgttta
    75301 tagagaaatt tttaccttta agtttatgac ttacaaatat aagattctta ttgtctgtta
    75361 aaaataaatc ctctccacta atttcaacat cttcatttgc taaccataaa gcagtgtcaa
    75421 taacatgtat atagtcatct ataagagtac tgtcgaatct aacatttctt aaggaattta
    75481 atccatgttt acatatattg atagatacaa tttctgtagc attattttta atttctttgt
    75541 acataggaca gaatctacgg ttgaatccaa ccattaaatt taagtttttt ttcgtactta
    75601 actcgataag ttcttctcct tgacttactg ttgaagcaag aggtttgtct acatatacat
    75661 gaacacctaa atttaataat atttttataa tttcgtagtg tgtttctgta gagctatgta
    75721 gaaaaataca atcacatttt ttagctaggg attcaataga atcgaatggc atgattctat
    75781 aatcactaca gactttttcc cttttaactt tatttggagt aaaagctcct acaaactcaa
    75841 atctctcaga cttagttaat ataggtaaat atgccttttg agctatggag ccaagaccta
    75901 tcattcccat ttttatattt ttcataatac tttctcctaa ggtttttata tttacagaat
    75961 tgattataat tcagacacat aaatttgtaa actttgtatc aaaaaataaa tttaaagctt
    76021 atataagtaa attagcgaaa taagaaatat tgataaagta attttgtagg atatccataa
    76081 aattgattga tataaatatt ttatgtattg acaaaaaatt caaattaatt tatatttgga
    76141 ttagtggtaa ttaccacttt ggtctgaaca agcttttatc gtaaaa
//
LOCUS       DAELNY010000002        64948 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.002, whole genome shotgun sequence.
ACCESSION   DAELNY010000002 DAELNY010000000
VERSION     DAELNY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.002"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(184..777)
                     /locus_tag="KPE55_000078"
     CDS             complement(184..777)
                     /locus_tag="KPE55_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG0491184.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(848..1711)
                     /locus_tag="KPE55_000079"
     CDS             complement(848..1711)
                     /locus_tag="KPE55_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG0491185.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            1874..3043
                     /locus_tag="KPE55_000080"
     CDS             1874..3043
                     /locus_tag="KPE55_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0491186.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            3144..3620
                     /locus_tag="KPE55_000081"
     CDS             3144..3620
                     /locus_tag="KPE55_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0491187.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            4186..4980
                     /locus_tag="KPE55_000082"
     CDS             4186..4980
                     /locus_tag="KPE55_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG0491188.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            5004..5447
                     /locus_tag="KPE55_000083"
     CDS             5004..5447
                     /locus_tag="KPE55_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG0491189.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(5536..6822)
                     /locus_tag="KPE55_000084"
     CDS             complement(5536..6822)
                     /locus_tag="KPE55_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0491190.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7040..7930)
                     /locus_tag="KPE55_000085"
     CDS             complement(7040..7930)
                     /locus_tag="KPE55_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0491191.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            8222..8566
                     /locus_tag="KPE55_000086"
     CDS             8222..8566
                     /locus_tag="KPE55_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0491192.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(8725..10821)
                     /locus_tag="KPE55_000087"
     CDS             complement(8725..10821)
                     /locus_tag="KPE55_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG0491193.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(10834..11994)
                     /locus_tag="KPE55_000088"
     CDS             complement(10834..11994)
                     /locus_tag="KPE55_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0491194.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(12013..13080)
                     /locus_tag="KPE55_000089"
     CDS             complement(12013..13080)
                     /locus_tag="KPE55_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG0491195.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            13432..13998
                     /locus_tag="KPE55_000090"
     CDS             13432..13998
                     /locus_tag="KPE55_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491196.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(14193..15179)
                     /locus_tag="KPE55_000091"
     CDS             complement(14193..15179)
                     /locus_tag="KPE55_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG0491197.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(15209..15904)
                     /gene="pxpB"
                     /locus_tag="KPE55_000092"
     CDS             complement(15209..15904)
                     /gene="pxpB"
                     /locus_tag="KPE55_000092"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG0491198.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(15924..17144)
                     /locus_tag="KPE55_000093"
     CDS             complement(15924..17144)
                     /locus_tag="KPE55_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG0491199.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(17173..17964)
                     /locus_tag="KPE55_000094"
     CDS             complement(17173..17964)
                     /locus_tag="KPE55_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG0491200.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            18552..19181
                     /locus_tag="KPE55_000095"
     CDS             18552..19181
                     /locus_tag="KPE55_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0491201.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(19295..20809)
                     /gene="glpK"
                     /locus_tag="KPE55_000096"
     CDS             complement(19295..20809)
                     /gene="glpK"
                     /locus_tag="KPE55_000096"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0491202.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            21053..21595
                     /locus_tag="KPE55_000097"
     CDS             21053..21595
                     /locus_tag="KPE55_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491203.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            21609..24101
                     /locus_tag="KPE55_000098"
     CDS             21609..24101
                     /locus_tag="KPE55_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0491204.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            24522..24920
                     /locus_tag="KPE55_000099"
     CDS             24522..24920
                     /locus_tag="KPE55_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0491205.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(25137..25346)
                     /locus_tag="KPE55_000100"
     CDS             complement(25137..25346)
                     /locus_tag="KPE55_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491206.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(25378..25587)
                     /locus_tag="KPE55_000101"
     CDS             complement(25378..25587)
                     /locus_tag="KPE55_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491207.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            25886..26305
                     /locus_tag="KPE55_000102"
     CDS             25886..26305
                     /locus_tag="KPE55_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491208.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            26533..26988
                     /locus_tag="KPE55_000103"
     CDS             26533..26988
                     /locus_tag="KPE55_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491209.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(27209..27469)
                     /locus_tag="KPE55_000104"
     CDS             complement(27209..27469)
                     /locus_tag="KPE55_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491210.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(27732..28085)
                     /locus_tag="KPE55_000105"
     CDS             complement(27732..28085)
                     /locus_tag="KPE55_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491211.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(28102..33423)
                     /locus_tag="KPE55_000106"
     CDS             complement(28102..33423)
                     /locus_tag="KPE55_000106"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491212.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(33440..34423)
                     /locus_tag="KPE55_000107"
     CDS             complement(33440..34423)
                     /locus_tag="KPE55_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0491213.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(34424..35122)
                     /locus_tag="KPE55_000108"
     CDS             complement(34424..35122)
                     /locus_tag="KPE55_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0491214.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(35119..36171)
                     /locus_tag="KPE55_000109"
     CDS             complement(35119..36171)
                     /locus_tag="KPE55_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0491215.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(36164..36592)
                     /locus_tag="KPE55_000110"
     CDS             complement(36164..36592)
                     /locus_tag="KPE55_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0491216.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(36592..36918)
                     /locus_tag="KPE55_000111"
     CDS             complement(36592..36918)
                     /locus_tag="KPE55_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0491217.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(36934..38463)
                     /locus_tag="KPE55_000112"
     CDS             complement(36934..38463)
                     /locus_tag="KPE55_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0491218.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(38745..39167)
                     /locus_tag="KPE55_000113"
     CDS             complement(38745..39167)
                     /locus_tag="KPE55_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491219.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(39167..41620)
                     /locus_tag="KPE55_000114"
     CDS             complement(39167..41620)
                     /locus_tag="KPE55_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491220.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(41620..41787)
                     /locus_tag="KPE55_000115"
     CDS             complement(41620..41787)
                     /locus_tag="KPE55_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491221.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(41814..42260)
                     /locus_tag="KPE55_000116"
     CDS             complement(41814..42260)
                     /locus_tag="KPE55_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0491222.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(42374..42802)
                     /locus_tag="KPE55_000117"
     CDS             complement(42374..42802)
                     /locus_tag="KPE55_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0491223.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(42816..43880)
                     /locus_tag="KPE55_000118"
     CDS             complement(42816..43880)
                     /locus_tag="KPE55_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0491224.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(43885..44328)
                     /locus_tag="KPE55_000119"
     CDS             complement(43885..44328)
                     /locus_tag="KPE55_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491225.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(44577..45017)
                     /locus_tag="KPE55_000120"
     CDS             complement(44577..45017)
                     /locus_tag="KPE55_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491226.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(45122..45319)
                     /locus_tag="KPE55_000121"
     CDS             complement(45122..45319)
                     /locus_tag="KPE55_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491227.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            45488..45808
                     /locus_tag="KPE55_000122"
     CDS             45488..45808
                     /locus_tag="KPE55_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0491228.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            45890..46396
                     /locus_tag="KPE55_000123"
     CDS             45890..46396
                     /locus_tag="KPE55_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491229.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            47097..47852
                     /locus_tag="KPE55_000124"
     CDS             47097..47852
                     /locus_tag="KPE55_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0491230.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(47995..48423)
                     /locus_tag="KPE55_000125"
     CDS             complement(47995..48423)
                     /locus_tag="KPE55_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0491231.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(48844..49044)
                     /locus_tag="KPE55_000126"
     CDS             complement(48844..49044)
                     /locus_tag="KPE55_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0491232.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(49227..50396)
                     /locus_tag="KPE55_000127"
     CDS             complement(49227..50396)
                     /locus_tag="KPE55_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG0491233.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            50632..51453
                     /locus_tag="KPE55_000128"
     CDS             50632..51453
                     /locus_tag="KPE55_000128"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG0491234.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(51529..52917)
                     /locus_tag="KPE55_000129"
     CDS             complement(51529..52917)
                     /locus_tag="KPE55_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG0491235.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(53052..53810)
                     /locus_tag="KPE55_000130"
     CDS             complement(53052..53810)
                     /locus_tag="KPE55_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0491236.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(53812..54528)
                     /locus_tag="KPE55_000131"
     CDS             complement(53812..54528)
                     /locus_tag="KPE55_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0491237.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(54530..55237)
                     /locus_tag="KPE55_000132"
     CDS             complement(54530..55237)
                     /locus_tag="KPE55_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0491238.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(55353..55889)
                     /locus_tag="KPE55_000133"
     CDS             complement(55353..55889)
                     /locus_tag="KPE55_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0491239.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(55991..57061)
                     /locus_tag="KPE55_000134"
     CDS             complement(55991..57061)
                     /locus_tag="KPE55_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG0491240.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(57054..58112)
                     /locus_tag="KPE55_000135"
     CDS             complement(57054..58112)
                     /locus_tag="KPE55_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG0491241.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(58099..58431)
                     /locus_tag="KPE55_000136"
     CDS             complement(58099..58431)
                     /locus_tag="KPE55_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0491242.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            58630..59751
                     /locus_tag="KPE55_000137"
     CDS             58630..59751
                     /locus_tag="KPE55_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG0491243.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(59879..61777)
                     /locus_tag="KPE55_000138"
     CDS             complement(59879..61777)
                     /locus_tag="KPE55_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG0491244.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            62092..62868
                     /locus_tag="KPE55_000139"
     CDS             62092..62868
                     /locus_tag="KPE55_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0491245.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(62960..63712)
                     /gene="xth"
                     /locus_tag="KPE55_000140"
     CDS             complement(62960..63712)
                     /gene="xth"
                     /locus_tag="KPE55_000140"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG0491246.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(63873..64823)
                     /gene="galT"
                     /locus_tag="KPE55_000141"
     CDS             complement(63873..64823)
                     /gene="galT"
                     /locus_tag="KPE55_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG0491247.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT        21572 a        10836 c         7277 g        25263 t
ORIGIN      
        1 gaaaaacaca gttttagtga gaagattaca atttaaaaca ctatctatta ttactaatct
       61 tcacaatata aaaaatcgca gtttagatat tccttataca aatttataag gaataataaa
      121 ttgcgatttt tatttttaag tagtacaatt aatttatact acttcagtat ttaaattcat
      181 tttttaataa tcatacttat tcgtagtttc gcacattact acctttattt ttttattaaa
      241 catattctta tattctaata aaaactcatt tagttctgaa tccataagat ttatttttct
      301 atcactacaa agcattatct tttcttggtc gattattaca ttaatatcat ttaagtcatt
      361 ttttataaat gttgttatac aactctttgg ttggattttt tctccatatt tctcataaaa
      421 tcctgcactc ttcttaaatg actctaaatc ttctgtagat actttgctgg aagtattatt
      481 actcataaaa cctgctgtag aagggtcgat tccaaagttt ctaagttgtt tttctacttc
      541 ttcagtgtcc ataccatatc tctccataaa ttgcttttgt attttcataa atttttcttg
      601 agatatgttg ttatctctca tataatcgaa tatttttctt gaaaactcag ccatagacat
      661 ctttccatcc ataacagaaa aatataagtt atatatgtat tgctcaaatt tatctacatc
      721 agaatcttta actttgttct ttagttcgtt aaagtctatt attctatcat cagacatttt
      781 tcatcctcct agctaaattt ttacttgtat aagattatac catatttcta ttatatttat
      841 atacacacta tttttttaat ttaacgattt tttctgtagc atctaattcg cacattctgc
      901 ccaaaggaat agttatcatt gggatacaat gtcctgactg aagattatat atagaaggtt
      961 tattgacctt atttgcaaat tctattagta attccatcaa atcattttct tcaatacaat
     1021 ccttaaaatc tccaaaaatt ataccattac attcctcaag ttttcctgaa gaatatagtt
     1081 gtgttaacat tctatccagt ttatattgag gttctccaat ttcctcaata aacaaaattt
     1141 tatcttttgc atctatttca tattttgttc ccattgtggc aattattaag gataaatttc
     1201 ctcccattat tattccctct gctttacctt cacatacagt ataaatcttt tcttcttttg
     1261 gattttctaa atataattca tctttaaatt ctaaagcatt taccaatgat ttataggtaa
     1321 atttacccca tttacctaca tttgctgcca ttattccatg atatggtaaa agaccacaat
     1381 ttttatagaa agctaaattt aaacctgtta tatcagaaaa acctataaat ggttttggat
     1441 tttgctttat tatatcataa tttattttat ccaatattct tgttgttcca taacctcctc
     1501 ttaagcacat aattatatct acatctttat ctaaaaacat atcttctata tcccttgctc
     1561 taatatcatc attaccagct aaataacctt tataatttaa actacaactt tcagaaaact
     1621 taacttcgta accataactt tctaaattaa actttatttc ctccaaactt gtatttctta
     1681 atggcccaga tggcgcaatt attcctaaca tttaaacacc tcttcttata aaatgatata
     1741 ttcttctcca tactaaatat ctctaatata attataagat attttatttg tacttgatac
     1801 ataataatga tttacataaa ataatataat taatgaagaa aaataaattt ttaatatagt
     1861 taggatgtgt tttatgctta atataaaaac tctttgtcat gagataaatg attgggttat
     1921 aaatataaga agagatttac ataagactcc agaacttgga ttagaagaat ttcaaactaa
     1981 gaaaaaaatt attaaatact taaatgaaat aggtataaat tatattgaat ataagaatca
     2041 tacagggata actgcataca ttaacgtgag tccaaacttt gaaacagttg ctataagagc
     2101 tgatatagat gccttaccta taacagaaga acttaattat tcttataaat ctattaatat
     2161 tggcaaaatg catgcatgtg gtcatgatgc acacactgct attttactgg gtacttgtaa
     2221 tatactattt aaattaaagg attacttaaa tgtaaatgta aaattctttt ttcaacctgc
     2281 tgaggaaact attggtgggg ctcaacttat gatagaagat ggatgtcttg aaaaaccaaa
     2341 tgtaaaatat atatttggac tccatgtaaa tcccaatatc aacaaaaatc taattgaatt
     2401 aaaatacaac actctaaatg cttccaccaa tacacttcaa ttaactgtac atggttcaaa
     2461 atgtcatgga gcttatccac atcaaggtat tgatgctatt gttatatctg ctcatattat
     2521 tacagctcta cagacaatcg ttagtcgaaa cacaaatcca actgattctg tggttatttc
     2581 tctaggaaag attgaaggtg gaatcaaaga aaatatagtt tgtgacaaag tggttataag
     2641 gggtactttg agaaccttga ctcctgaaac tagagagttt tcaaaaaaac gaattagaga
     2701 aatttgcgat tttacttgta aaacatttgg aggaagtatt tctgttgaaa ttgaggaagg
     2761 ttatccagct cttatcaatt caaaccatct agtagattac gtcaaacaaa atgctgtaga
     2821 attgtttggt gaagaaaata ttatccttaa agactcgccc actcttggtg ctgaagactt
     2881 ttcgtatttt ctaagacact gtgaaggagc tttttaccac cttggttgtg caaatcgtga
     2941 aaaaaacatt acttcccctt tgcatacctc tacttttgat attgacgaag attgccttat
     3001 tactggtgtc atgctccatg taaaaaatgt attatcattc taactattaa tatttttatt
     3061 tattatttta tattgaccct cacatgatgt catgttatac cctaaaatta caaatgataa
     3121 attaaatagt tggaggtaat attatgaaag aaatattaat aaatcctata ggtaaaatat
     3181 gtattcaagg cgaagaggtc tttattaaat tagacaaaaa gtatgtacct gcattaaatg
     3241 aactcgacga tttcagtcat ttaaatgtat tttggtgggc tgatgggttt gataatcctg
     3301 aactaagaag tattttagaa accccaaaac cttataaaag tggaccagac gttataggaa
     3361 tatttgcaac aaggtcacct ataagaccca atccaatagc attgactgct gtacaaatta
     3421 tcaatatcga ccatgaaaat ggcataataa aaataccata tattgatgct aatgataata
     3481 gtcctgttat tgacttaaag ccatatactc caagcgttga tagggttgaa aatccgtccg
     3541 ttcctaaatg gtgtagtcac tggcctaaga gtgtagaaaa atcaggagaa tttaattggg
     3601 aagatgaaat tgaattttaa attacttttt tcttgatttc attaacttgt tttccacatt
     3661 tattttattt acacttacca agattttatg tcacatatac atataatatt aggctgtatt
     3721 atataataca acctaatatt atataataaa tttctatttt tcccttaaat cattcacatt
     3781 ctttctaagc tacataagat ttttctatta tttctttttg agtttaattt agcattatgt
     3841 tgaggaaaaa acatttcatg cattttaata aattaaattt ttcttttatt ctgcgatttt
     3901 ataataatca atttaactta aaaataatac ctaactattt taactatgtt aaactagtaa
     3961 atgtaaccac ttatatgtta caattttata taaacatcaa atataaaaaa ctatataaat
     4021 aaatgacaca tagtgttata tttttttata ccaatttagt tatttttcag ttacagattt
     4081 aataatagtt aatagtaaga cataatattt ttatgccctt tattgattat gtattttctg
     4141 ttatctgtta ttatcatcaa taataaacat aagggggtat atattatgaa attgtattta
     4201 agtgctgata tagaaggtac ttgtggtatt gtaaattggg atgaaacaaa acttgaatgt
     4261 gaattttcgc aacactatag aacgcaaatg agtaaagaga taacagcagc atgtgatgga
     4321 gctactcaat ctggtgtaca tgaaatactt gttaaagatg cacatgacag tggtagaaat
     4381 attaatccaa atattttacc tgaaaacgtc aaaatactta gaggatggac caaagaccca
     4441 ctagttatga tggcaggaat tgatgaaagc tttgatgcat gtatattcac cggctatcac
     4501 tctggagcta cttgtaatgg taatccactt tctcatacta tggatattga ttatgactat
     4561 tttaaaataa atgatgaaat agcatctgaa tttactataa atgcttatat tgctgcatat
     4621 tataatgttc ctgtagcatt tttaagtgga gatgaaatgc tttgtgagag tgcaaaaaaa
     4681 ctaaatccaa atatagtcac tgttcctgtg tcaaaaggaa ttggaaatgg ttcaatttca
     4741 atccatccaa accttgcact aaaaaaaatt gaaaaaggtg tcaaaaaagc tttatctgga
     4801 gatttatcta ggcacttaat aaaattgcca gataaattta agatagaaat aaaatttaga
     4861 gaacattata aagcttttaa ggcctctttt tatccaaata tgaaaaaaat agattctcaa
     4921 acagtactat ttgaaacaga ggactattat gaatttttaa gaatgttatt atttatataa
     4981 atttcatatc ataaattaag gagttgatta agataagtaa taaaagaagg attcctttag
     5041 gcgaatttat ttctgtattt ttattttttg ctgttttttt tggtataact ttttgtattt
     5101 tcacggttat aggaattggc ttcttatcat tcttaggatt tgaatataaa tctcttggag
     5161 ctgtattaat tttcttttta atatattttt gtataaccac tccaattgat tttctatgca
     5221 ctactatttt agatatattc agatatgtaa acaaattacc atactcaata tataaacttt
     5281 gcgaatttat aattgatttt atactaactt ttctagctat gaacataatt gataccttta
     5341 tggattcagt aacgattcct ttaagtacag aaatcttatt cgctttactt tcttatcttt
     5401 tatcagaatg tatggatttt tttgataggg ataagaaaaa accttaataa aaaagataga
     5461 ttccttaata gaacacatat tttcgtattt ctattattta atctatcttt attttattta
     5521 ataaattttt attaattaaa atatagtttc ttctatctcc ttagtcattt gcttagcact
     5581 atcaaaaaat tcttctatat acactacttc atcactgacc ctagaatatt ctgctgcaaa
     5641 tgccattaca tgactttgta tagcttcttt tgctgctact ctaccctttt ctagttcttt
     5701 atttgaaact aatttaataa attcagctat aagatttcta tcatattcct ctaccatatt
     5761 ctgttgattt gatggattca taactatttc ttcgtttttt ccaaattctt taattcttat
     5821 ctcattttta tttaaatccc caccaacttc accatggcta aacattaact ttatagttct
     5881 tccattttct ttagtaaatg cacataaatt aaaagtagcc gtcactccat tttcaaactg
     5941 tagtatattt accatattgt ctacaacatt attatcacat ctgtaaacac atcttccata
     6001 tggacttgtc ttaagtatat tcaatagatt ctcttcagtt ggatttatgt gaacagctct
     6061 atttatactt ctatcttttt ctaaataaat tctctttgct gaatatggac attttttttc
     6121 aactaaacac tgaagacaat tttgtgccat ctctccacta gcattttggt tattaagatg
     6181 ctttaagctt ccaaatgcag atattttttt gcatctactt cctactaaat ataacaaaat
     6241 atccatgtca tgacaactct ttgctaaaat taaagggctg gtttcattgc tatttctcca
     6301 attccctcta gtaaagctat gtgcaaaatg ccaatatcct atattttcat tatattgtat
     6361 actaactagc tctcctaatt ttttgctttc tacaatttct tttaatttat tataaaatgg
     6421 agaatatctc agtacatggc aaatcataaa aatcttatct ttatacttat cacacaagtc
     6481 atctatatgt accagaccat ctaaactatt agacattggc ttttctaata gtacatgata
     6541 acctttttca agagctagtt ttgctacatc ataatgtcta tcatcatttg tagctattat
     6601 tactgcatct gccatcttgt catgtttaaa aaagtcttct aaagtttcaa atacattttt
     6661 actatctagg ttatattttt gtgtaaatag ttctcgtctt ccttttttag gttctgctac
     6721 agcaactatt tcacatacat cagaattttc ataaatatat ggagcatatg catacgttcc
     6781 tctattacct gctccaatca cagcaacttt cactttttcc atactttaat ttatcccctt
     6841 tatattattt atactactct attatagtct atttttacag acatttggat atttttaata
     6901 aaagaaacag cagttataat ttttcaattt taactgctgt tataagttaa tataccaaac
     6961 atttttaatt aaattttctt taattttaat acaaagactt aaatattaaa tacaaattat
     7021 aaaaacactt ttatctagtt tacttcttct tttctttgtc taatttatct aacttcttat
     7081 tgtataaaaa catataaaat gctgccgaaa atactattaa atatccaaca acacttaaca
     7141 tatctggata gacattgaat aagaatatac ttaaaattgc tgaaaagatt atattactat
     7201 agtcaaatat agagatttcc ttagcaggtg catatttata tgctagagtt attccaaatt
     7261 gacctagact tgcaaatact ccacctagta atagatatat aagttgcata agggtcattg
     7321 gttcatatac tgcaaaagca attggcccta cagtaactaa tgaaaaaact gaaaagaaaa
     7381 atacaattgt atagtaatct tctctatctc caagaactct taaacaagta tatgccattg
     7441 ctgcaaatac agctcctgca attcctccaa gatatggaat tacttctaca ctaaatgttg
     7501 gttttattat gaataaagct cctataaatg caattataat agctcctatc tgctttacat
     7561 ttattttttc ttttagagca attgcacaaa atattactac aaggaaggga ctcattttat
     7621 ttagcatatt agcatctgaa agcatcaact tgtcaattgc atagtagtta aatataactc
     7681 ctagagttcc aaatattgac ctatataaaa gtacttttaa attttctttt ttaccagtta
     7741 gacttccttt atgttttatg ataagccaaa aagctattat agctgatacc agatttctaa
     7801 agaacacttt ttgaaaactt ggtaaatctc cagctagctt tacgaatgca gacatcattg
     7861 caaagccaag cgctgatgct attataaaca ttataccttt tattcgattt gatttgattg
     7921 tgctactcat acgtacctct tttctatata aatgtataaa gccatattaa ttaaaaactt
     7981 agcttataca ttaaataata attatctaaa tattccaatt tatactataa ttaaaagtgt
     8041 aaactatgtc aagtaactaa taaatctttt caattaataa caataataat tatagctaaa
     8101 aagttatatg tttttatgga ttattatcag aatatataat ataattttat atagtataga
     8161 ctttttttaa tttattattt agatttttaa tagatttttt aactatggag gtatttaaaa
     8221 tatggaaata aagagatatg aaggaactgg aagaatgagt agagctgtag ttcataacaa
     8281 tacagtttat ttatgtggac aaactcatgc tgaaggagga gtaattgaac aaacaactga
     8341 agttttagct aaaatagaag atttattaaa caaatatggt tcagataagc aacatttgct
     8401 atctgttact atttatttaa gagatatgaa agactttgaa gctatgaact ctgtttggga
     8461 tgcttgggtt gaaaaaggat ttgaacctgc tagagcttgt gttgaagcta gacttgctag
     8521 agagcatttg ttagttgaaa tgagcgttgt tgcagctaca aaataatcaa tttagcttat
     8581 aggaggctat ctcaaaacag agagataagc ctctttttca ttgttgaaat gatgaaaata
     8641 tatttgattt cgtataaaag aaagtgtctc acgaactaaa aaattcattt tgagacacct
     8701 cctcactatt taatctatat aaatttactt attctctatt ggcaatatca atttagatgg
     8761 atatttacct ccatggtaaa tcgtattctt tgcttctata tattcaatac tattatatcc
     8821 ctctgtcgtg ttgctatttt ggaaaataaa gtttttagca ctagacgtta tatctagtct
     8881 aattttatga ccaactttaa acgtgtttga tatttttgaa gttataatcg taaacttata
     8941 aatctctcct tcttccataa actctgactt ataaaaacta tttctaaatc tagcactaag
     9001 tataccatca gctaatttaa ttgaatttcc atctaaatcc acgtccataa tttttataac
     9061 ccagtcagta tctttggcag aacttgatgc aaaaaactct aactttatat cacctgttac
     9121 agtaaattct tcactaaaag catctgatgt ataacacaac atatcacttc ttttttctaa
     9181 atctttgtag ttatttggaa ctccaacttc attctctgac atatcaatta gttgtactga
     9241 tgggtctttt ggattatata tataactatc atacttttct tctaaatcat tatcaaatac
     9301 taatctacca tttcctaaag atgtgttagc atttccatca cttattagat acatactttt
     9361 atctatctta tttattattg gccaattttc ttctgtcttc cacttattag aacctacaga
     9421 gtaatactct actggagcag tcgtatctat gttattttct atacctttta agtatttatc
     9481 aaaccataat agataattat aatccaaatc atatcttaat gaactatttc ctaaagaaat
     9541 accatttata tctcttaaag tatttgaatt atgcatccat ggacctagaa tagcctttct
     9601 ctttcctttt tcatagtcct ttataacatc taaggcctct gtagttccca tactattatc
     9661 atcataccac ccagatacaa ccaatgcagg tacatttatt ttatcttttt gtaaatgcca
     9721 gtccatcaaa tcccaatact catctttttc tactctcttt aaccattctt ccagaaattc
     9781 aattctatat ccaagagctt caacaggtat attttctatt ggtcgtatgt ttaaaacatc
     9841 atcccaatca tctcttacca ttttgcttct atcaacttta tttcttgata ccataaatgc
     9901 ccatgcaagc ataccagata caaaagctcc tccttttctt ggtatatcaa taaaagggct
     9961 tcctgatgtt actatactaa ctaaagcttt aagatgctta ttgcctgaag atgcagctgc
    10021 ccattggaca taaccaagat atgatgctcc aagcattcca actattccac ttgaccattc
    10081 ctgtgatact atccaattta tggtactatc tccatcttcc ctctcatgaa tcataggttc
    10141 ccacttacct tctgattcat ttcttcccct aacatcttgt attacaacag catatcctct
    10201 ttgtacatat ttataatata tttctttatc attttctttt ccatatggag ttctcataag
    10261 aattgtcggt gcttttttgg cagaatctac aaaatcaggg agatatacat ctgtagaaag
    10321 ttttatacca tctttcatat caatcatata atttccacca aatcttatag aatatatctc
    10381 ttcattttta tgagtttttt cccattcttt taatacagta tactcttcat atccatccat
    10441 aattaatata gtacaagttt ctctagataa acagataaaa cctactattt tattatctac
    10501 aataattata tctagtggga actttttgtt tctttgaacc catatatatt cattaagttt
    10561 agaatgcgtt tctcctaagt tatttataac tgtgtcttta tctgtgttta ctaagctttt
    10621 agtatataca tcaaaattat taaataaaga ctttaaatct atttttgtaa gatttaaata
    10681 atatgtattt tcttctttgc ttattggttt taaactttct attttatttg taaaaggatt
    10741 caacttttca attaataaat ctccatcaaa gattacattt ccactgtata gtccagatat
    10801 gtataattta aattttatca attaaactcc cccctataat cctagagaca tctttattgc
    10861 atatgccact gatatacctg cataatttcc taatactcca ccaattactc ccattacgac
    10921 tgctacactt acaagcgaat tccactttgc agaagcagct actaaagctg aagtaggacc
    10981 atcagcaatt gctcctacta tagaaagtat tacatattga tactttatct ttaaaagtct
    11041 agttatgcct aaatggaaaa caaatgaagc aaaaattata ctaaaacagt aaagtgttat
    11101 agtaaaagtt gaacctaaaa actcttttat atctactgaa aaacctattg tacataaaaa
    11161 gaataatgca acaaataatc ctaaatctaa atttcccttt agtttcttta ctggctttaa
    11221 ttgagcaatt ataattgaaa ttgttgtaac taaaagtatt cttgctgcac tactaaaact
    11281 tgaattgaaa tatccagcaa gtattgttga aacttctgta acaacaaaac ctatagccaa
    11341 catccaagct atatctttaa tactccattc ttttgactcc ataaattctt cattttgaca
    11401 atcttctaat tctgattctt ctacatgata tggccaaagt tttttaaact tctttgaatt
    11461 ttttaatatt gctggtagag caaacataaa tatcaatgtt ggtactccaa ttacatagtc
    11521 tgctgcattt gctgctgcaa gagtttgtct acttacatta agacctgttc caatagctgt
    11581 aagattagca cttccaccag tatatgtacc tacaaacatt ccagcaacct tccaaccttc
    11641 tgatattttt tctgcaaata ctaatccaaa taaaaatgcc atgatactta cagttaatac
    11701 agctacaaaa attgcaatta aaggttctct tgataatttt gtcatttctt ttaagtcaac
    11761 actcatcaac attatagtca tagaaattgg aatagcatat gttgaaatag ttccatataa
    11821 ctcagtactt actggaacta cttttaagtt tgaaagtatt attcccatta tgattattgt
    11881 aagtgctggt cctaagcttt taatggcttt gtatctttgt aagtaaaaac ccaatgcaat
    11941 cattgatgtc attacagcaa gtatactacc tggatttgaa aaaagtgtat tcatatttaa
    12001 cctccaaatt tatcataagt ttacttctat tcctaaacct ggtttattga caagattcat
    12061 aatatctcca tccattacaa atcctccaga tataccttct aattcttcac aaaacataaa
    12121 actatcaagg tcagcttctg ttatgttctt tttagatgct attaaatttg ctgctgctgt
    12181 tataccaatt ctcgtttcca gcatacatcc taacatacaa tttactccac ttgcctcagc
    12241 tatgttgttg atttttatag caggatatat tccacttgat ttcatcagct taatattaaa
    12301 tgtatcaact gcattctttt ttatagcctt aattgcatct attggtgaat gtaatgattc
    12361 atctatcata atttttgtgt tacttttatt tcttatgtaa gcagtaccat ctaaatccca
    12421 atgtggaagt gcttgttcaa ttgcatcaac attaaattct tctaactttt tgatagtatt
    12481 tatactgcta tttacgttcc atccttgatt tgcatcaatt ctaattctta tactctctcc
    12541 aacggcttct ctaattaact ttacagcttc aatatcgtct tcaggattta tacctgcttt
    12601 aagcttcaat atcctaaaac cttcttttac tctttctaaa gcttctactg ccattttttc
    12661 tggcttatct atacttatag taatatccgt ttggacttta ctatcaaatc ctccaagaac
    12721 tttataaaga ggtgcattca taatttttcc tttaatatca tataaagcta tgtcaatagc
    12781 tgcttttgca gaagtattac ctataataat tccatccatg atggtatgta tggtttcaat
    12841 atctagtggg tctttgccta ttaactcttt tttaaacata agcaatactg aaagaacatt
    12901 atccaaaact tcacctgtaa ctgctgcaag aggtgctgct tcgccaaatc cacaataccc
    12961 ttcatcagtt tcaattttta aaattatact ttctccatac tcaattactc caaatgaaac
    13021 tactacaggt ttttttaatt taattctaag cttttcaaat ttgatatctg ttatcttcat
    13081 attaccctcc ttattttttg aagttataat tcaattttta aaattttata ctttaaatta
    13141 tattattact tcaatatata tgtcaagata atttactgaa aatttactct tttccaaaat
    13201 ataatgtgat aatcttaatt caattaatac tcttctatta aattttttta ttattatttt
    13261 ctttgtatta atttaacaat aattcatttt catagacttt tatttggatt taattaaatt
    13321 ttaattgtca aaatctacta tgtattctta ttgccactac ttctatgttg tgctataatt
    13381 actttgtttt aagcgaatat tttatattaa ttttattatt tggaggttct tatgctaggt
    13441 gaaaagatga gaaatataag aaaaagcaag aaaaaaacat taagtgatgt ttccaaattg
    13501 acagatttat ctataagtta tatctctcaa atagaaagag atgctattga accatccttg
    13561 tcttcattga gaaaaatcgc tgaagtttta gacactcctt tatatatgtt tatggatgac
    13621 aataatactg atgatttagt cattagaaaa gaagatagag ttatgatgaa atttccaaag
    13681 agtgaaatgt tttacgagat agtatctcca atgcctacaa ctgagtttac accttcaatt
    13741 ttatttgtag agtttgaact taaaccagaa agtgaggatg ccaaagatta tatatatcat
    13801 gcatcagaag aaattatggt ggtaacgagt ggtgtggttg atatttgtat gggtgaaaag
    13861 gtagttagat taaatccagg tgactcaaca tttataaaag ctaatgtacc acataaaatc
    13921 atcaatccaa gtaaagatac aatagctcgt ggatatggag ttatatcacc tccaatttgg
    13981 ccaataaaat ctaaataaac tcaatatgac acacctactt tttgtagata atataataga
    14041 tttaaatatt gcttttattt taaatttaaa atatttactc aaactaaaaa aatagctatt
    14101 tgactaatct aaaccatctt aattatggta aatagtaaat agctatttgt aagttatatt
    14161 ataaattata ataaaaattg tatttgttaa aattaaactg ctatatcatt taatactttt
    14221 gcctttacaa ctcttatatt tttcatactc ttgtagatat cttgaatctt atcttcttgc
    14281 tctctcaata gtctatgtgc ttcatatata tctacaagtt caaattttac tacatcattt
    14341 ggctttgctt gtgctaactt atacaaatct acacttataa cgttccctat cttagtatag
    14401 ccaccactag tctgtctatc tgataacatt ataatcggtt ttccatgacc aggtacttgt
    14461 atagctccat aagaaatacc atcagatata atatctgctc cattaaggtg ctttatactc
    14521 tctccatcta gtctacaacc cattctatct atttcattcg taactttgta tttatttgat
    14581 aaaaatttct ctacttcacc gttagaaaaa gcatcaaatt gaggaccttt tacaattctt
    14641 agtacgattt ctgaactata agttggaata tattttgaac ttaatttttt atttattgta
    14701 aaatcttttt cctgaataaa agtatttata taatctcctt ttttaagtgc tctaccttca
    14761 aatccaccaa ttttagctct tgtataagtt gacttacttc ccattgcaag tggaacatct
    14821 attccaccag ctattgacac ataggctcta gcacctgatt tcacacctct aaatgataat
    14881 acatcacctg gatttatttg atatgacctc cagttttgaa tttcttttcc atttatttta
    14941 gcccctaaat ctcccccagt tactgctatt tgcgtatatt catcaaaggt aattgtagga
    15001 cctaacatta aaacttctaa agaagcctca tcttcagaat ttcctactaa tatatttcct
    15061 actctatgtg catactcatc cattgctcca catccagata caccatattg accatagcca
    15121 actctacctc tgtcttgtat taatgtttgg aaacctccta attcaacttt gaatcccata
    15181 tataccactc cctgtactat aacgatattt aaatacttaa gttatttttt tcgatttcta
    15241 taaattcatc taatgttatt ggaacaaatt ttataaaatc tccagctcta agcaaaatct
    15301 gttcttctct attttcatca tataatttta aaggtgtcct accaattatt tgccatccac
    15361 caggactatc aataggatat acaccagtct gggaacctgc tattccaaca ctcccaccat
    15421 aaattttagt ccttggcact tctaatctag gtgtagctat tctttcatcc ataccaccta
    15481 gatatggaaa tccaggagta aaacctagca tatatatcaa gtactctctg cttgtgtgta
    15541 tttttataac ttcatctaca gtgagattat tgtagcttgc aacgttttta atatctatac
    15601 caaaaacctc atcataacat actggaatct catgtatttt aggttttggt aatactatac
    15661 tttccaaatt atcttctatt ttttttaaat tctcaatcaa actatcaaaa tctatttcta
    15721 atggattata acttatcata agtgacctat atgttggtac catttctgtt acaatacctt
    15781 taagattcga tatttctatt gctctcataa atgaaattac ttttttgttt atatcttctg
    15841 atatctcatt tccaaattca gcaaccactg ccttatcccc tgatatcaag tatctagttt
    15901 ccataaatgt cacctcttct aaattaagca aataaagcac ctaggctgct aagagaagtt
    15961 attcctgtat atgcagatat taaaactact attaatccaa atattaaaag ccatgtagga
    16021 tgcttgtaat ctccaacaat atcttttctt ttagatgcta taagcataat tcctaatgtg
    16081 attggaagta tcaatccatt taaagctcct gctaaaatta ataaagtagc tggttttcct
    16141 ataaatgcca ttataagtgt tgatattgct ataaatgcta ttataaaata tttttcatat
    16201 ttatctataa aaggatttag tgtctttaag aaagaaactg atgtataagc agctcctata
    16261 actgatgtaa ttgcagcaga ccataataca agtccaaaga atttatatcc tattgctcct
    16321 gcactaaatt taaaagctga agctgctgga ttttctgggt caagttgaag accttttgaa
    16381 accactgcca gtattgctaa aaataaaaga actctcatca ttgtggcaac taatataccc
    16441 aaaagagaag atttagttat ttcttttata ttttcttctc ctgttattcc accgtctatt
    16501 aatctatgtc ctccagcaaa agttatataa cctccaacac ttcctccaag taaagttatt
    16561 attgggaata tcagtccttt tggattttct ggttttacca ttcttgatac tgcctctcca
    16621 acaggaggat gatttgatat tgctacatat ccaactataa ttatcattcc taatgctaaa
    16681 aatttagtta tcttatcgac tagagaattt gaattttttg acataaatac aaatatagct
    16741 attaatccac ttaatacagt tcctaatgtc atattcatat taaacattac attcatacca
    16801 agtgcagctc caccaacatt tcctatatta aaagctaatc ctccaagagc tactaaaaaa
    16861 gctactaaat agcctaatcc aggtattatt ttatttgcta tatcctgacc tctaagtcct
    16921 gatacaccaa ttactctcca tacatttact tgagccccta taaaaaggat tgttgtaatt
    16981 aaaattacaa atgaaaaatt cggtccaaaa tcttgggtaa attgtgcagt ctgagttaaa
    17041 aaccctggtc caattgctga tgttgccatt ataaatgcag cccctataag tgctccgaca
    17101 tcttttttat ttttagtttt atctgtaata ttttttgtac tcatatttta tctccccctc
    17161 atgttatcag tcttatacta tgttctctga cgaacaaact tctatgtttt ctaatggaca
    17221 aacttctata ccttctaatt caaaacgctt tcttatttct ttgacaaact ctattgcttt
    17281 tggattgtcc ccatgtacac atatagaatc tgctttgata tgtataactt cacctgttaa
    17341 agtttctaca gttccttctt ttaccatcct aacaacccta tctattgcat gttttgtatc
    17401 atgtataact gctccttcta catttcttgg aaccaaaaaa ccattattat cataagctct
    17461 atctgcaaat acttcatttg caaacctaag tccaatttct ttagcagaat ttatcatttc
    17521 actattatat agccccaaca gtataatatc tttatcgact tcatatatag cttcacaaat
    17581 ccccatagct aactctttat ctttagctgc catattgtat aatgctccat gtggttttac
    17641 atgttgtata ttacatccat ttgaacttgc aaatgccatt aaagcaccca gttgatattt
    17701 tgtataatct ttagcttcct gctttgttat tttcatctca cgtcttccaa atcctattaa
    17761 atctaggaaa cctggatgtg cgcctatttt cactccattt tttttagcta gctgtactgt
    17821 tttttccata tgactagggt cacctgcatg aaacccacaa gctatattgg ctgatgaaat
    17881 atattttaaa acttcttcat ctaaaccaat cttatacgtt ccaaaacttt ctcctaaatc
    17941 actatttaaa tctactttat acaatttatg acccctcctc gctaacattt tgatagaata
    18001 agtagtttta atttttaaat tttgtttatt ttatatattg catgaaatat tttatcactt
    18061 gtattcaatt ttatttttgc aactattcta ttgcatttaa ttaatataat tttaccattt
    18121 aaggaatatt tttgcaatta ttttaattga acattcattt gtatttcttt ttcaaaaaat
    18181 ctttttacca ttttttagtt aataattaat tattttcact taaaataagg gctttaacct
    18241 tcatttttaa agtatttttc atttttaatc actttatatg caagacagaa tcatttttct
    18301 tcattcattt gtttctttca tctatataat tatcattctt tgtaagtaaa atatctttat
    18361 atttttataa ttaattcttt atatagtttt aagtataata aaatcatgta tactaaatgc
    18421 atgttaaaat attattttaa aacatgcaca atatttgtac atattgtaga catataaacc
    18481 tataaattat acaatagtat tgttaagatt atatattttt ttaacataaa ttttgtattg
    18541 ggggaaaaac aatggataaa aactatacga tgccaatata ccaaaaaata gcgttggata
    18601 ttgcaaataa aatatacacg ggtgaaatac aagaggattc agtactattt ggacgctctg
    18661 ttttggctgg taaatacaat gtatctccag aaacgataag aagagctgtc aaaattttag
    18721 aggacattgg cgttgttaaa agcataaagg gtaaaggtgt tattgtacta tctccagaca
    18781 aagcttcttc ttttataaaa aaatatagag atattacaaa tatatcatca tataaatcaa
    18841 cactttataa tttaatagat actaaatcta atttagaaaa tgaaatatta gatactataa
    18901 ataaaatttt agactattct aacagacttg aaataatcaa tcctttagtg cccgttcagt
    18961 tcaccataaa ttctaattgc aaatatattg gtcaaacagc tgcacaaact aaattttggc
    19021 aaaatacagg tgctacaata gttgcaatta aaagaggtga agaacttata atatctcctg
    19081 gaccatatat tgaattttta gaaggagaca ttctcttagt agttggtgac caacacattt
    19141 acaattctat accaatgttt ttatatgaaa acgaaaaata aattatataa tttagtgtga
    19201 ttatataaaa atgcaagatt aacaacaaaa aaagagcttc atattttaca taaagctctt
    19261 tttaaattca aataaattta actttgatta atttttaaat atccaattca tcttcttttt
    19321 cccagcttaa agctctcgat acagcttttt tccatccttt atataattta catctcttct
    19381 cttcactcat atttggatta aactcttttt ctactgacca ctttgaagta atttcatttc
    19441 ttccaatata gaatccaaca gacaaccctg ctaaataagc agctcctaaa gctgtagttt
    19501 ctactatttt gggtctatct atatctacat ttaatatatc tgattgaaat tgcattaaaa
    19561 aattattatt acttgctcca ccatcaactt ttaatgattt tagttttaat tttgaatcgt
    19621 tttgcattgc ttctaaaaca tcctttgtct gataagcaat tgattccaat gtggctctta
    19681 ctatatgttc tttttttgct cctcttgtaa gtcctaaaat tgaaccccta gcatacatat
    19741 cccaatatgg agctccaaga cctgtaaatg ctggcactac atatacacca ttagtatcct
    19801 ctaccctatt tgcatacatt tcactatctt ttgcagattc aattatcttt aattcatctc
    19861 taagccattg aacagatgct cctccaataa atatacttcc ttctaatgca tatgttactt
    19921 ttccatcaac tccccaagca attgttgtaa gtaacccatg cttagattta actatgttac
    19981 tgcctgtatt cataagcaag aaacaacccg ttccataagt attttttgca gtaccttctt
    20041 caacacaatt ttgaccaaat aatgctgctt gttggtctcc agcacatccc gcaataggta
    20101 ttttagctcc tgataacatg cgctcatctg tatgaccata aacatagcta gaaggtttta
    20161 ctaatggcaa catgcttatt ggtatgtcca attctcgtaa tatatcctca tcccattcta
    20221 aatcatttat attgtacatc attgttctag atgcatttga gtaatctgtt acatgtactt
    20281 ttccacgagt taaattccat attaaccatg tatctatagt tccaaataat aactctcctt
    20341 gttctgcttt ttccctagct ccttctacat tatctaatat ccattttact tttgttgctg
    20401 aaaagtatgc atctattaaa agacctgttt tttcttttat tgattcttcc aatcctttat
    20461 ttttaagttc atcacatatt tctgacgttc tcctacactg ccatactatt gcgttataaa
    20521 taggtttacc agtataccta ctccaaacaa ccgtagtctc tctttgatta gttatcccta
    20581 ttgaagcaat ttcttctggt cttatacctg ctgtttctat aacttctctc attaccccac
    20641 tttgtgaccc ccatatttcc atagggtcat gctctaccca tccagctttt ggataaattt
    20701 gattgaactc tttttgtgca attttcacta tttcgccttt tttattaaat agtatagctc
    20761 ttgaactggt tgttccttgg tctaatgcca ttacatactt cttttccatc accatattct
    20821 ccttatcata taatatttaa tttattaaaa agaaaaattt aatatcacat atttcaataa
    20881 ataatcatac ttaagtacaa atttaatata atagatattt agtaagaaga cacttatttt
    20941 ctatcatatc tatataataa tattatacat tatagcttac atattgatta ataaatttta
    21001 aaggagtata ataaacagtg ttcataataa ttttaataaa agaggtggta atatgccaaa
    21061 gattttagaa aatgtaaaag aagatatatt aaaagtgtct cgggatatga ttttagaaga
    21121 agattactcg aatattagca taaggaaaat agctcaaagg tgtggtataa gtgcaggtac
    21181 agtgtataat tactttaatt caaaacaaga aataatagaa tatatcacaa aatctgaatg
    21241 ggatttattg ataaggcgaa ttgaatactc caataaaaac actgaagatt acataaaaaa
    21301 actaaacatt atttttactg aaataagaaa ttttataaac aaagttcata atatccaata
    21361 caatgacttt ttaaacactt ttgaaacaga gagatttttt gaaatgaaaa aacacaaaga
    21421 cgactttcat gaacagcttt caaataaagt atatgaagct ttagaaaaag aaagtttttt
    21481 taataatgac aaattagtat gcaatatcat aataaaaatg tttttttcat attcaacata
    21541 tcttcatata gaatttgaag atttaaaacc ctatattgaa aaattaatac attaaggtgg
    21601 tgtttacctt gaatcataat actagtatta agaacaagga cagtaatcca aaaatatttt
    21661 tagtacttac gctttatcta ttaggtatat ttatgggagc tattgatact ggaatagtat
    21721 caccagcaag aacgattata caaaatagcc ttggagtaaa tgaaaaaacg ggaatttgga
    21781 tgattaccat atacacatta acatacgctt ctgtaattcc tatctctgga aaattagcag
    21841 ataaaatagg aagaaaatat gtgtatctag ttagtatatt tctatttggt agtggttcat
    21901 taatatgtgg attatcatca ctattttcca atttctatat tttattaatt ggtcgtgtta
    21961 ttcaagcaat tggtggtggt ggaatcatgc caattgctac agcagaattt ggaactacct
    22021 tcccagaaaa taaaagaggc atggctttag gtttagttgg ggcaacatat ggtattgcca
    22081 atattttagg ttcaagcatt ggtagcacaa ttctaagtat ctttggaact caaaattgga
    22141 agtggctatt ttttgtaaat cttccaattt gtttaatcat acttattggt ggagttttct
    22201 gtattaaaaa caataagtct gaatctaaag aaaaaataga taagcttggt acattgatgt
    22261 tgatatgtat aatcgtatca ctattatatg gtcttatgaa tatagacttt tttaatttta
    22321 aaaattctat acaagatata tcagtttatc catacttatt gacatttatt gtacttatac
    22381 ctattttcat atttattgaa aatagagcta aagaccctat tttaagcttt gaatattttt
    22441 taaatccgag aatattgata gtacttattt tatctttaat tgttggtatt ggaatgatgg
    22501 gtatggtatt cgttccacag tatgctgaaa atgcattaaa gataaatgct ggttctgggg
    22561 gatattttgt agctatattg ggattatttg caggtattgc tgctccttta agcggaagac
    22621 ttattgacaa gtttggggca aaaaaaatac tattactagg attttctgta tctatgatag
    22681 gctctcttta cctagtttta attgctctaa aaaccaatac tgtatttagt gtatgtgtaa
    22741 gtttaatatt tatgggatta ggaatgggat ttactatggg aactcctcta aactatatga
    22801 tgttatcaaa tacaagatta gaagaatcta attcagcact tgcaacatta tccttgatac
    22861 gctcaattgg tacttcaatt tctccagcta taatgattgg atttatagca catgcaggat
    22921 tatctgtgca agataatatt atggacttag ttggaaagcc ctcaactcct aaaatagttc
    22981 aacttgaaga acttgaaaac atgatagatg acttaaaatc agaccctgaa atggcaaaaa
    23041 atttaaaaaa tgtctctatc ccaaatatga atgatacttc aaatataaat atggacatga
    23101 caggtggcaa acttccaaaa tccttactta ataaagttca gtcagcagat acaaccaaca
    23161 ttaccaatat aacaaaagaa atatcaacaa caatgtttga tgaaaaagtt ccatctataa
    23221 ttactaaaat acagaaaaat gtacaaaaag gaatagatgg aacacaaagt ggaataaacg
    23281 gtattgaaga tggtgaaaat aaactaaata gtggtattaa aggagttcaa actggtattg
    23341 acaatatggt aaaagctaga gctggtatta atcaaggaat agatggtgta aaaaaaggta
    23401 ttgctggtgt tgataaaggt ataaaaggta tggaacaagg tcttgaaaaa caagatgagg
    23461 ccatcaagga acttacaact gcttataata aaatacctac tacagaaaaa actcctactg
    23521 ataacgaaaa taactcaaat gaacaaaacc agagtaatac cagtaattct gaatcagacg
    23581 acactaagaa tgattctaca gaaaataaga ataacaattc cagcgagaat aaaccaaatg
    23641 taaatgaaca aaaagaaagt ttaaatgctc aaatacagca actaaaaaag acaagagatg
    23701 acttaaatac aaagttacaa aaaaataaaa ctcaaaagaa cgaattaagt aaaaaactaa
    23761 gatcaatgga atctcaaaag aaagagctac aaagcaagtt aaataattct ataagccaaa
    23821 agaaagacat ggaaaaaagc cttgatacaa tgcaacagca aaaaaaatca ctccaagcag
    23881 ttttagcaaa gacacaagag gtaaaaactg aaataccaaa ggcttttgat aaatcaaaat
    23941 tggattatat aaattccata gaaaataata gaacaaaaat agaaaacaca ctacaatcta
    24001 ctctaaactc aggatttaaa cagatgtata taacagtatt ctgtgtaaat ctactagcat
    24061 ttataatatt attgttctat aaagaaaata aaactagata aaagagtgtt atagtattat
    24121 attgtaaatt tatcttattc ttaaatattt tattctaaag aataaattca taaaattttt
    24181 gtaattgatt aatataaatt acaatataat actatttttc tcttaatctt tttctctttt
    24241 acagacaatc aaaaagcaat cttagtttat cgaaataaat ttatacaatc tattcataca
    24301 cccctttaca tttatataac aattaaacct attgttatat atacaaatca aaataaacca
    24361 tatattttca actataatat gttaaaatca tattatgagc atattaattt aagcaaaatg
    24421 tcatttagac tttaatttag tagttttata ttataaattt atatattttt aattttaaat
    24481 gtcatttaga cattactcat aaaaagaaag gagtgtattt tgtggatact ttaggaaaaa
    24541 gaattgctta tttgagaaac tccaaaaaac ttactcagcg caaacttatg gatattttaa
    24601 aatttgaaaa cttaggcaag tatgaaacag gagatagaaa acctaattgt gatatactaa
    24661 tgtcaatagc tgattatttt aatgttacga ctgactggct tctctatggt aaagagaagg
    24721 taaatgtcaa tagtagtgta aaagaagata aagaagatta cttacatgtt actaatgatg
    24781 aaatgacgat attaaatcta tacagacaac taaacgaacg tgataaaata aaaatagaag
    24841 gaatacttga acttaagata tctgaatata aagacttaaa aaagcattcc tctaataata
    24901 atgaagataa aatggtttaa acatacataa tttgatacga ttttacatca tctattaacc
    24961 ttcttttttt gtagtatatt actaaaatat ggatattgga ttatttaaaa acaagtatag
    25021 tctaatttca agcacattta taatatttag attatcttat ccatatttta gtttaagtac
    25081 tttgaaaaat ataatacatc tctatgtata acttatattc ctaagaaatg cattactcat
    25141 gtttttaatt cttccaacat ctttctatac acaattgtac atccttcata attcccctct
    25201 ttaagaagga ttgaacatgt actagaatat ttagttaaag tatttaatct ttccatattt
    25261 aagcttttta tattactgag tgctgtaata aaatcatcaa ttgctctaca acactctata
    25321 accccatctg aatccaattt acacacaaga atattcacct actttatata ctatttatta
    25381 atcatcttct gaatattcta tattaagtaa ttgataaatt gatgatttat attttttacc
    25441 tgttcttctt ccatttaaaa tagctgacaa atagttttca ttaacaccta ttttcttcgc
    25501 taattccctt tgagtcatat ttaattcaat aagtctttgc ttgacttgca ctcccaaaaa
    25561 aggtatccat ttttttcgag catccattta tcaacttcct ttctaagtga tttatattta
    25621 cttttatatt attttagctt acacaccccc tctatttagt atatattttt aatatgtaca
    25681 catattaagt atataaattt atttaatact aaataagatt ttttgattac tttgtgctaa
    25741 aattaggatg taaattcgta tcattaagtt aattataaag tcattttgac cttaagtcaa
    25801 tgctttttat gtcattttga ccttaaattt gaaactattt acatcttaaa aagtcttttt
    25861 gacattaatt gaatggagag tgtttatgga tactttagga gaaagaattg tttatttgag
    25921 aaaagcaaaa aaccttaaac aatatgaact agaagaaatg ttgggctgtg acaacttaag
    25981 taaatttgag agaaacatta gaaaaccaaa ttatgaaata ttaaaatcta tagctgagat
    26041 atttaatgtt tcagtagact ggctattaaa tggagataat ctatcacata aatcagattt
    26101 aatttgcgat tcctcctcaa actatccact taatagcata aactctaatg aaataaagct
    26161 tttaaacaat tttagaaagt taaatgatta tgataaagcc aaaatagaag gtatgattga
    26221 attaaaactt catgaatatg aaaaagagaa ggatttagga aaaattgagt ataacaaaaa
    26281 taaagatgag aaaatagata aataattttt atgttgcttt tgatacgatt ttacatcatt
    26341 tctttgttct aatattttta tttcatttat tttctttttt ataattttat gataaaataa
    26401 ttagtatatt ttgctttact tgaatgtaat tatttataca aaagtcaaac ataatataaa
    26461 tgtagtaaat ttttagtttc aagtaatatc tcgttattat aactaaatat ttattaaaat
    26521 taggaggata aaatgggctt aagagacaaa ttcgcgcaat catttgctag atcaaaaact
    26581 atgagtggcc ctgagaaaaa ggctaatgaa ataatgggta aattgcttct taagaaagct
    26641 atattaccta tcgttttaat gttcgtgata ataatcgctg gagctatgct taaaatcaat
    26701 tcttgggtta cacttggcat aaatttagtt attgctgttg gtgctttctt ctacataaga
    26761 aattctagta aaaagtatca gaactttaaa ccttatgttg gcaatttgat tagtttagag
    26821 aaaaaaggta aaaaagagta cgttgctatt ataaaacaag gtaagttacc agttaagtta
    26881 caaatagctt atggtggtga agaccttgaa catgttaaga aaaatcaaat ggttcaaata
    26941 agttataatc cagatgctaa aatagctatt ttggttaata gacaataatt taataaaaaa
    27001 aaggagttgt cttaaaattg attttttcta atcaaattta agctcaactc cttttttaat
    27061 ttttatatat tatttttata tattattttt attacttttt ttaattaata tttttaaata
    27121 tcaaatcctt ctcaataaaa tagggtacta tctacaatac tactttatct tttcctaaca
    27181 atatataatc tggtgctact ctttcatgtt atacaccgtt ttgtaacata ccttttatac
    27241 cagtaacatc ttccttaatt tcctctacat ctgttttcat tatacccatt tgaacaagta
    27301 tatttttgtt tatttcttgt tgttgtgtag ataattctat aaaattttct acagtttttt
    27361 tatacatgtc tctatcttct tttttctcct gcatagtatt tttaaataat agagcacata
    27421 gtatacctat tgcccctaga ctactcaatt cggttattaa tttatccatt atagcctcct
    27481 attgtctaaa ttttttatac ttatagcttt aatttttgtt atattcttac atcatgcaat
    27541 attatatttt caggattatc aatttaatct tctgtacttt aaattgtagg tgcaatttat
    27601 agattgaata aataataaat atatatacaa tttctaatat aacaaaaaat aagtacaagg
    27661 cttacttatt aaagttaaga ccttgtactt tacataaaaa aaataactaa tacatagtta
    27721 ttctaaattt actattcaac tggtatttct aattctttgc atctaattct aacttgctct
    27781 ctaattactg ctggtacttc ttctattgtt tttttgcctc ttaaaattaa agctacatat
    27841 acttctacca ttccactctc acctccagat tttcttacac taaaagagct tatattactt
    27901 gcttcattga cagaactttg atttaacaac atctcatata tttctgcatt tgctataaaa
    27961 ttatttgtta tatcatcatc ttgctttact tgacctatac ttatgtctga aatgtcttta
    28021 tactgcattt ctacttttct accttgtttg atttgttcta ttttattttt atcgaattta
    28081 aacaattttc acacctccat tttaagcttg tggctcgttg ctttcagcat catatctaaa
    28141 aatcatagat ggacgtttta tagctgtcca tttatctcct ttttctacta tgattttagg
    28201 ctgagcactt tttatgttgg cttcttctaa ttcatagatt tcttggtaac ttgagtctga
    28261 ttttttgatt tcaactagtt tcatgtcttt gaataagtca gatttattta tttttacttg
    28321 aactttagtt ttatttatta attgatatat aggattgttt tccatcatgt catcctttaa
    28381 tccctcattt aatgttacta ttaaatcagt gttattttca gtaatgttta tgattgaata
    28441 tactttattg tttatcatta aaatagaatc cttttttact ttaagccctg tttctgattt
    28501 aaatataaac atacttgaat ataaatcgcc tgtaggacta ctggctttaa tcagtatctt
    28561 attttctcct aatactattt tattttgtgg tatctcaaat ataatgctat ttatttccaa
    28621 attagtctta gtagatatta attcatcatt tataaacatt tcaagttttt ctataacatt
    28681 attagggtct tgtactacaa ctctcacttg agatattgat ttttcaacta tttctataga
    28741 tgctttactt gtatctactt ttgttatcat gtatattgta tcatctgaaa gctctatatc
    28801 taaaatatca tttagatgtt caggaattgg tattttagta aattcatata aatcagtaat
    28861 tgatatagat attttataca tagatttatt tttaccagtt atccatagac tattatcatt
    28921 tttaattact agtatgtggt catcattatg tgacttaatt tctttaacat tagttaatac
    28981 tttagtaaaa gttcttatta tcttatcttt ttcactttct cctagtccaa ataatccatt
    29041 tgtattgtac ccttgagcgt acaactctcc ctcattagtt aaaacaagtg tcattgagtt
    29101 atatatactt atatctttaa cagaaggtaa tccagtaacc ttttgtaact ctcttacatc
    29161 attattgtgg ccaactccca attggtactc agtattcttt ccatatgacc atagagtatt
    29221 atctgtctta ataatatagg agtagttatc tccacaagca acttttttga catctgatat
    29281 atttacttca gtaagagtta ataattctat atcttcaagg gtttgattaa tttctttata
    29341 tgaattactt cctattccat ataacttgcc atccttagca acagccaatg aatgtgtttt
    29401 tgatatatca atatcagata cctcaaatgg caatattatt gaagtatact taaaattatc
    29461 tttaaatatg tttttataat ttatatactt attatttgtt tttatatact tatcattaaa
    29521 cattactagc ttttcatttt tcttattaaa aactatttta tctgatgtgt tatctgtttt
    29581 aaaatatgaa tgtagggaac cttcaagagc ttcattttta tcattatcat atctatggtg
    29641 aatacctaaa tgattatatc tattatctcc tgttgaagaa taaaattttc catatatatc
    29701 tttaataaat atagtattgt aactagaaaa tacagtatca acatctttct ttatagatac
    29761 tttataatat ggaactgaca tatccctata agattcatca gatataccat aagtatctct
    29821 acctgatgat atcaaattgt tattattatc tatataaagt gtattattag ctgttacaaa
    29881 aacatcttta acatcttcca cactatttgt attattatta tttgctatac ttccatatcc
    29941 actaagacct ccaacaaata gtttattgtc atttgttaaa atggctgaag tatatccatt
    30001 aactactatt ttttttgcat ttataatatt attaattact cgtataccct ttatatcatt
    30061 agtttctgtt gaaaagaata ccctctcttt tttccctgta gtataaataa cattgtcatt
    30121 tgttataaaa aatgtagctt cacttcctgc tgttacaaca gattttacat tctcaatatt
    30181 tatttttgta aatgtactta tacttccttc agtaaaccca aaatatccat ttacaaatcc
    30241 acatacataa catgtattat catttttaac aattattgat acatcattac atatatacac
    30301 atctctaact ccatttatag gtatacgtgt aaatatattc ctacttgtag tatcacccaa
    30361 tcctaactgt ccttttccat tattacctgc tccatataaa agaccatcat tagttaataa
    30421 tagagatgtg tttccatata cagctacata ttttacattt tcaacattta ttttagtaaa
    30481 tacatttcta tttacattat ctcctattcc taactgacca gaattattta aaccaacagc
    30541 ataacaggta ttatcatttt ttatcgcaaa tacatgttta ctattgtcag taaatctatt
    30601 tacatttata gattttatat tatcaatatt tattttagta aatacctttc tatttatctc
    30661 atctcccact cctaattgac ctacatcatt taaaccaaca ctgtaaagca tattattatt
    30721 agttaataaa tatgtagact tcgaatggct atatatttct tgtatattag aagatatttc
    30781 actactgcta ttctttttat tgtaatttat cttattattt aaatttaaac tactattaac
    30841 tgatatacca tgcgtaccct tagagttata tccacatata aataagctct tatcagttaa
    30901 tattgcaaat ctagttttat ctctacttaa atcataagga ggtataaata tattttttat
    30961 atttttcatt acctttttat aattagtggc attatatcca tcttgtgtat ctgaaacatc
    31021 tagttctttt gcactttcag atatattccc aaaaccatat aacgtgttag aagataaata
    31081 caatagagct ttatataaaa atatggcttt atcagaggta ataggacctg aatttatatt
    31141 agatgctgca atattattag atgattgatt gttaagataa ttccttccat attgatgtat
    31201 ataagatgaa cctccagaaa taagtaaatt atctggattt acatttttta aattacttgt
    31261 tgattctcca taatacaaat aggcaaaatt tgtagaatat gatgattgtg tacaccttgt
    31321 gggataatag ttatatatgt aattactaat atatccagca tgtgtaattc cttgttgttg
    31381 aactctataa gtagaatcac ttggctttgt ccataaagta ccatggtatc cagttaaaaa
    31441 agaaccataa tttgtacata atatagccat atattcatat ataataatat tattaatttt
    31501 agtaatatca tttgtacaag aatttgtact ataacttgct cctgaaggat ttacataaaa
    31561 ccagttattt attaatgtta aatttatatt gactccatca ttaggattaa cacccaataa
    31621 atagtttgta tttataccag ttgcatatac ctttttttcg tcagccacaa taaatgttgc
    31681 attatatgaa ctatatgcat ctgttacatt gctaacacca atctcagtat attgacttgc
    31741 tgatttatta ctaagtccat taacataact tgaactcttt ccagaagtat atatttgctt
    31801 attattagtt acaattaatg aaaaccagtt tgataaatca gtatttataa caactttttt
    31861 tacattatct atagatattt tcttaaatat atctgaatta gaaggaagaa cacctgctat
    31921 tgagttacta tttggcccag aataataaca agtgtcatca tttttaatgt agaatattcc
    31981 tccagaaata ttttttgatg ctactataga ctttacatta gagatactta attttgtcaa
    32041 ctcatttaat tgacttggta agtcagaacc cattcctttg tatctattat ttcctagacc
    32101 atatacatct ccattcccta aaataaccag agtacaataa ggactaaaaa cgacatctct
    32161 tacagaatgt atatctagtg gaagttcaat atcctttatt tccatttctt cttttaataa
    32221 agttaattta tcctcagatt taattaaatt tatataatta ctataactat atttttcact
    32281 atccattttc ttaaattcta caatgtgcat atcatcaaaa aacgaaccta attgtccata
    32341 agtattatct cctgtacaat atgaatcatt atttgaatca attagtatgc tatgtgataa
    32401 accaatgaaa atattcttag ctacaatatt ttttaaatct ataaatgaat tatctatatt
    32461 ctcagagtaa tttgctatac ctaactgata tatatcatta tttccagcaa cctttaaagt
    32521 acctttgtta tctataagca tagtgttacc atgaccacat gcaatagttt ttatatttac
    32581 ttcttctatc ttagtaaata gtgatgattg aagtgcattt ccaaaacatt tttcctttcc
    32641 acaaccatat atatcattat tatacgtcat taacacagtg tgttcagaac cacaagctat
    32701 agtcttaaca tcattaatag aaactaaact aaataaatct ctattattct tatctcctaa
    32761 acctagttga ccttgattat taaaaccagt agaatagcat ttattgtcat tctttattat
    32821 aaaagctgat ttattatatg aaaatacagc ctttacattg tcaacatcta cttttgtaaa
    32881 tgtatttaca tttgcagatg ctccattagt tcctagacct aattgaccat acaaattatt
    32941 tccagcaaca tataaagttc catcattgta taaaagatat gttgattgaa gtccacatgc
    33001 aatatccttt acgcctttta tttctggaac cttctcaaat gtagaaactg ttttccctac
    33061 tttttctaat cccaatccca tttgaccatc tgtatttata cctgcgacaa gtaattctcc
    33121 atctgaagta agtataactg catgagattc tccacatgca acttttttaa tattggtaga
    33181 tatatttggt attttcgtaa ataattttct tgttgtattg tcttctccca atccaagttg
    33241 aaagttacta ttatctccac atccatacaa gatattatta ttttttagac caaatgtata
    33301 gttacaacat ccagtaaaaa aattaataaa gtcataatta tcataccctt catcaaattc
    33361 atcaactacc atatttttat cgccaaagaa attacctctt tgaagtagtt tagttctttt
    33421 cattataaaa acctcctaat tatcttattt catacgaata tttatttaaa gttatatctg
    33481 tctgaccaat tgaatatgcc ctattagatt ttgtttcaac aaagttatta tccatttcat
    33541 gtatatatct aactctattt gcattaaatg gtagcaccac attcttaaat gtaacactac
    33601 caagttctcc agttatacac tttctattta aatctaactt aaggccttct atagctatta
    33661 tgtttttagc attagttaag gtatcatacc aatatccagc ttcatctaca tctatttctg
    33721 actctaattc taagtatatt aagatatcta atactttttc agataattta ttgtaactat
    33781 tttttaatac agatagttca gtttgtattt gacctcctat tacatcatca atagatatat
    33841 ttttaatttt ttctactacc tcatttatag ctcctgataa gtttttagct tctgtagata
    33901 agagttcttc gccaattttt acttttattt cttttatatt tttatcaaac ttatcatcta
    33961 aatcttgaat atccttttga gttacaaaaa ccaacgtcgg gtctacttct aatgtaacat
    34021 ttgaagtatt ggacaattgt aaaatcattt ttacaaccaa atctttaata ctgccatctt
    34081 cagcacgtgg cttatacgtc tctggatact tacctatagc tattatatta ttctcattat
    34141 ccagaacagc agcttctctt atcataaatc caccaactgt tcctggtaaa atagtctcta
    34201 taactatcca attaggattt ttttcatcaa ttcttagaga atttatattt ccttcccaaa
    34261 ctacattctt taaagcagtt tgttcctctg ttggctcata atattctcca ttactatctc
    34321 caacttgaaa cttggttaat ccaacaagct ctcctagtgc agttgcattt gctatttttt
    34381 ctctaccaat tttggttaat attatataaa atttttcttc caattatacc acctccgatt
    34441 taggataaac tactacattt tctacacctg tatcatttgc aatagctata ttaatcttac
    34501 aattattttc aatgcattta atgcttaaag gataaacctt tatttcttct ctacttatca
    34561 ttccagctcc acagtttaca acagcactac attttataat tggttcaaag tatggatgaa
    34621 cttttacctc ttcactacat accatacctc caccacaata gaccatactt ttatctagta
    34681 ttattggttc taaatagtgt aataagtgag caggttttac tctttcaatc atatcgctac
    34741 aatctaaaag agttttatag tcacagttat ttgctataaa actcaatacg aatgtaaatt
    34801 catcactata aacttttata tctgtttctg attttgtata tgcctcacat atacttttta
    34861 taacttcaat agtactagta cctctgcttc tcattttagc ttttatattg ctacgtcttg
    34921 tgtcaaaatc aagttctttt ttttcaatgc agagtatgtc ttcccacata tctaatcccc
    34981 atgttgcagt gtcaacaaat aattggttaa aggtatcatc aatatcttct ttaagaatat
    35041 ttaattctgt atcgtatgca ccttgtatct cctctacaat gtaatttcta tcaaatgatg
    35101 gtagtttatc aattagcttc attgattttc cacctcacta aaattaacag ttgttacact
    35161 aggtatttta tcttcattaa tagttatatt atctgtactt ccatttataa gtaggttact
    35221 taaatcgtgt acaccagtag tatttataag tattcccatt acttttgtat agattatctc
    35281 tcctctaata tctctaaagt atgtatttat actttctagg aaagattctt ttacattgtc
    35341 taatgtatat ccatcttcta gtttcattac tgcacttata cttatttcta ttggtaatgg
    35401 tgtcacaact gttatagttg gtccaatagg cttctcttca tctatatgtt gctgacacct
    35461 ttcaattgtt tctgtatcaa cagcttgatt atttttccca aatatcaaga ccttaactgt
    35521 tcccggacca tcccatcttg gataaacctt aacattatag actccatcta cttccaaagc
    35581 ccattcttca tagtgagctt tatttccact tgtagcttga tttctttgga ttttataaaa
    35641 tctttctttt agttcttcat ctgtctctat atctgtacca ccttcaaaac ttcttgtgtt
    35701 agtaatcttt gttactccag atatattatc aactagttta aattcacaat ttgtaggtaa
    35761 attatatttc ttaccaactt ccagagcttg aactgggcta ttgtcacctt cttcactacc
    35821 aatagttaca tcttttatta ctacaaatag taaatctcta tatgatatta ttgtgccatt
    35881 atttattaca gtacctttct ctccaataaa ttcaacttct ccatttgact ctgtacctaa
    35941 ctttctatat acaccaaatt cattgactct tttatcaaga aactggttat atgtgtcttg
    36001 tataaaagcc attttatgca tctttgaaag ttctagatat atcttcgaaa gttctagatt
    36061 atttccagat accatgttgt ttagaaaaga accttctcct ttataaatat caagattaat
    36121 attttcaaga gttctatttt ttattacttc atatgtctgg tcactataca tttatttcca
    36181 cctccccata gatagtggat acttttacat ttgcactcaa tatgtctctg ttaaagttag
    36241 cactttttac gtttacttct aatatatatg gatttattag aagggcctct tttatgaatc
    36301 tactagcttc actttctgta agaccttttg tatatttttg tcctattagt tctgaaagct
    36361 ctgtcccata ttctaaagag tatatctcat gttcatatct atttgtcttt atacacttgt
    36421 atatccaaac ttttaaggct tctttttttt caattatttt aaagtcacct ttttctaaaa
    36481 taggttcatc tttttcaaaa tcccatgcca cttcacgaaa gattggcaat tcttctgttt
    36541 taggtaagat ataatcctct gggacaccta taaaaggaaa tattgtactc attataagct
    36601 caccaactta cttacaacag caaatttttc gcctattcta aacattatta ccatatcccc
    36661 agattcaaag ttatctataa aaggattttt tacttcatgc tggtgttctt gatttgtttc
    36721 tgtgtcaaat gtttctagct gtctatcaat catccaacta tctatcaaaa tatcttcttt
    36781 ttctaatatg atgttatttg tctctatttt taaatctggt aatttacttt taatttttcc
    36841 aataaaaaaa gaaggttgat tatgatactt tccttcttct cttattattc ctataaattc
    36901 atttattgga ttagccatta tatcaccatc cttttataat actctcctag ctgtgttaaa
    36961 atctcttcta ctacttaact tacttatttt aactacatca cctttttgtg gagcatgtaa
    37021 aaattctcca ttacctatat ataatccaac atggctgact ggattatgaa aaaatactaa
    37081 atctcctgct tgaagatttt tttgttctac tttcttgcct tttttagatt gttgatttga
    37141 cgttcttggc aaactaacat taactttttt aaaacaatag accattaaac cagaacagtc
    37201 aaaactactt ggtccatttc caccccattt ataaggttta cctagatgtt tttttgcttc
    37261 tgatattact attttagctt tatcagatac attaccagaa taaccaccaa taataatttt
    37321 tccttttctt cttccaaatt tactagcttc ttcaacattt ccaaataaaa tatctatatg
    37381 gtatgttcca ttcttttcta tagttattgc aggtcctacg tcatttactt tatagactcc
    37441 atcacgacca gaaactcctg taattacctg tatttcatct ccatagttta ataaaggatg
    37501 tttatttaaa aactcttttg tataataagt ttgctcatat ttaccaacca taggagcagc
    37561 gcaagttttt ttagatgggt caagtttttt ctttctacaa tctgtatctc caccttcttc
    37621 ttttctagga cagtaagctg taaattctgc tgtaaactct tttcctcctg cataatcttc
    37681 tccccctaaa ttactttctt ccttaggttc atcctgtcct gctgactttt catccataag
    37741 attttgaaaa ttaagttcaa gctcaatttg atattctcca ttttgccaag tatgtttgtc
    37801 tgtatctata taaaatagtc ctacaagctt tgtataagaa tctttaactt ttactcctct
    37861 accagttata caacttacat ctccataacc tttaagagaa cagctttttt ctatcccatt
    37921 aaactcgcta tcaatatcta catcttgatt ttcttgttgc tgaattactt tttgcattat
    37981 tacatttact tccttaaaaa tttcattatc tattttttcg ctaatcttgc ttccatactg
    38041 gtctaccact attactttat tttttacatt ttccatgctc tccgaaaagg tggtatttat
    38101 aatattaaat ccctcttcaa acataacact taatgtaaca gttccttttt caataacatt
    38161 aaacttatct aaattggcct ctatcatata ctttttcttt gtctttttac ttgcttctgt
    38221 atatgcactc attattgtgt cataaccatt tactcctata aacatctttg tatacttgac
    38281 attggtcttt gctattattc caactgaaag cctattttca gcaaatactt gctttgctat
    38341 gtcttcaact aacttatctt taaaattgta tgacacttca ctttgtgtaa gtaaaaatcc
    38401 catatcttta gatacaaaac taatttcatt actgcttgaa tctttagacc tattaattat
    38461 cattcctcta aagagttctt tctcatctac atagaaacag actgtactag ctattggtat
    38521 atcgatttgt tggaaattta catcaaatga tgattgtatt attgaaaact ctagtgtcct
    38581 tgatggagat ttataatcac ctgaccatga taccttgtct actatgtcag ttatgtcata
    38641 tatacttccg ttttttatgt gtacccaaat cttaataata aaaaatcacc tgccttttca
    38701 tttaattcta tcagcagtta tttataactt attatctata atagttattg ttttggagtt
    38761 acttttggta tctttattct tttatactct tttaaatcta atgtaaaata tacatctcga
    38821 gtcccatctc tttcttcata actaaaccct tcaattatga cttccatatt tatatttgtt
    38881 tccgtaattg taaatcttaa tataaaacct tccttcatcc atttttctat cttatttaca
    38941 caatcatatg gtgatggaaa acctgtatag tcacagaaag tatagtcttt atttggaaaa
    39001 aaacttgata ttgaaatgct tttaagagct acaccaccaa aagttgctat ttcacctatt
    39061 ttgagtatac tagaagagtt tacagcagct tttccattaa tactaaagga agatggaaat
    39121 actggaaatc taaatctatc ttcagcttgt ctaagccaca tttccattat aagacacccc
    39181 ctaactgtgc caattggata cttgcaacta attttgatgt tatcttttct atatcagctt
    39241 cttctctaat cactattgta tcagctaact tagctatgtt tatatttcca ccattttttc
    39301 tgcttctata ttgatttgct tcttgtttag ttaaaaccat ttcaccttca tggagtctgg
    39361 tttgatagtc attataagga acatagttca atccaaatgc attattacct ttcttacctg
    39421 aataagcttt accagaatca ccagcctttg ttccagcata tggattatct ttgtaatttt
    39481 ttttactctt ttccactaga tttaaaactg ttcctgtgat gcttccattt tttatcttat
    39541 taatcatagt ttgccaccat ttacatatat ctttaactat tttacatact ttatctaata
    39601 tgttaaaaaa agctcccaat attggctcta taattttcca tccagtttcc aacagaggtc
    39661 ctaaggtctc ccatacagtt ttccaaataa cacccagtgt ctctataaaa ttgtttattt
    39721 gaggtgcata ttcacctaaa aaagtaaaaa ttcttgtgat aataggacta acagcttcaa
    39781 ctacaggtcc gattagttgg aatgctgtag ttactattgt accaaatata cttattaatc
    39841 cttgtgcaat tggtgattta ttgtttatac tatctagtaa attatttact aagtttacta
    39901 ctggaagtaa tgaatctatt aatgctgtaa aatcgatatt ttgtaatagt gtacttcctg
    39961 cgttaacaat tgcttttggt gcattcccta tcattgttgc tgcatgattt tcaaaacgtt
    40021 cttgcttatt ttcaaccatc attttacgct tttcagcctc attagaaatt cgatattgag
    40081 tagaatcaga aaactctttt ccatttttta cacttctaga ttgcatctgg tcatatttgt
    40141 tgccaataat tatcttacca ttcattacat tgcctatttt accagccatt ttttcagata
    40201 agccagctaa attgcctaat ccactttcta taaaagtttt tattttcttt agtccttgac
    40261 ctaaaatatc atcaaaaggt ttaacaatac tcttcaagcc attgttaatg cttgtttgag
    40321 ctcctgatat tatacctggt attgtattac tcatttcttt agctaacccc atctgcttta
    40381 cacttatgcc ttccaatgct tcatcaaaag ttttataatt ttctcctacc agagtattaa
    40441 gagattccat gtttcctttt gctgcactta agaaagctgc tgatacatct tgttcactac
    40501 ttgtattcat atttaaagct ctaacatcta ccatagcttg agttatatct ttttcttttt
    40561 tgccaccagt aatattacta atcattttag ctttagttcc cattgcaaca acatcttcag
    40621 ggtcaaaagg cgtatcattt gccattctta ctaaactgcc ataataatca tcaacatcta
    40681 tttgattaac ttttccagta tttcttttca ttacactttc tagaacaact ttctgatttt
    40741 gttcctgacc aagtaaatca acaccctttt tcacagcgac acctatagta gcaccatttg
    40801 tcaaatcaga tatcttccct tttatcgaac caagtattcc acttgccata tcctttacag
    40861 ctattgcacc actccatacc ttacctgcaa ataatctcaa tttgggaaat actttactta
    40921 taatgccact tgccatatct tttatagcta ttacaccact ccaaaaagta tttttaaaca
    40981 acattaactt aggcaaaatt ctacccatag cactactcac catatcttta agattaatac
    41041 ttccttgata aggacgtttc tctacttgtt ttaattttcc caagatacta cttatcactc
    41101 tcttagtcat atcttttgct tttattacga tattccaaat cttgccacca atctttttta
    41161 aatttccaag taatttattt ttagtttttt ctacttcatc acttttagct ttaaatatta
    41221 atataatcat aatatttgaa ttaaactcat ttttcttttg aatcaactta ttaaaataat
    41281 caataatcat agctatagtt attgctagtc tcataatact tccatttaaa cttccaataa
    41341 ctcctgaaac agcatttatg ctagaactta attttgtcat agttgaactt acactactag
    41401 cagtagtttg aacttttctt aacgaggaat ttaaactgtt tactctatta cttgcacttc
    41461 caatagaatt ttttatgcta ctagccatta cattagtgga aagtgaaaca gccacagttg
    41521 ctctcctaaa attatcagtt actgatataa tttgattcat ttttggtgtg taattatctg
    41581 taaggtacat atttatacct tcttctctca catttcccat tacataccac ctcctgtcaa
    41641 aggacacact ccatatccat gtttcatttt ttcaacttct tcctcaattt ctaaggcgaa
    41701 aaaagcttca ataagcttta attcgccttt atccattgca taaaaaaggg acggtcttat
    41761 accttttttc ttccaataat agtacattat tgtagtcctg gtatccgtcc ctattagttt
    41821 tttacctctt ctatcactgc atttttacca taacccataa catcttgaag tattctatat
    41881 aagttggtaa tttcacctgg taataataat tttcttgcta attcttttgg tgttggagcc
    41941 ttaaatttat ccattagctc cttattttta aataaaagct ttccatccaa atcacaaaca
    42001 ccttctataa ttgtaagcat ctgcatctcc ggtaagtcta tatccgtttt catattttca
    42061 tcaattttaa gacaagtatt ttgtatttct tcatctttac ttattgttaa agctctacac
    42121 attactttaa attcctgccc aaatatagtt gataatctct taagttcaac tatagtactt
    42181 ggtctttcta agttttctgt atctgaattt aaaagtaaat ctactatgtt tatatttttt
    42241 gataatccat tttcactcat tattatttgc tcctttatca atcaaattca atcaactaac
    42301 ttttttactt attaactatc tgaatttatt aagtatatac ttactaaata tcagtataaa
    42361 actaaatata aaactaaatt atatcaagaa aatcatactc agtaaatgtg aaaggagctt
    42421 ctatctctcc ttttactcca acctcccaat cagctaaagt taaatcatca aatgctatat
    42481 tttttactgc tattctttct gctccataag aatctgggtc atttaatttt gatatagcaa
    42541 caaatctagg ttcagaacct ctctttatct tttctccaat taacttgtgc attcttgaac
    42601 taacgtggta tagagttatt gagccttttc ctttatatcc catatacttt gtatcagtac
    42661 ccatttgacc tgctattata atatcttctt ttgtaaattc catctttgct tgaaatttct
    42721 ttacttctgc tactttgttt ccatcaagcc aaagttctcc ccaagtacca ctcattacat
    42781 ttctagcttc catatttgcc atattaataa tcctcctata tttctattga taaatctata
    42841 tcttccatag catcaagtac ttttattttt gcttttaaaa atactttaga acctgtgtta
    42901 gcttctttta tttcttgtaa tgtcatataa gataaatcta ctccttttga ttttaaatac
    42961 gatttttgtg cttcaaaatc tatttcaaca gtagaatcag attctataag tgctgatttt
    43021 tctaattctt ctaaataact ttttatagct actatcaata aacatttgtt gtcataactg
    43081 ttagtaacct ttcctatata gtcatctatt atcacctttc ttatgtcact atgtataata
    43141 tctaaagtgt caactatttt tattttctgg aacatttctc ctttttctgc tgttaattca
    43201 gttaaagaat ttactcctct agcaattctt atagctcctg cttctttaat aagtataagc
    43261 tctcctttat taacccttga ttctgcatca actttcgtca tcttaggtat atcgactaca
    43321 tcactaagtt tagtataagt tactgattga cttaaaggtg tacctgctat aagtccagcc
    43381 accctgcttg taaactcatc aacactgtat ttcttttctc caactaacac atcttctgta
    43441 gtaaaattaa ttatcccttc atgatttcct acaacttttc ctaatacagc tttaacctta
    43501 accttatcta tatctctaag tttaattatc caatttttta tagcagtctt atcagcttct
    43561 actgcttttg gcatacatag ataattaaat tccttagtct ctaaaaaatc taatgcagtt
    43621 tgaatatctg cttctccttc tattacataa actaataatt tatttggagt gttaacattt
    43681 cccatcaaag ctaaatttat atattcttta ttttcagcag ataaatcaac tggtatatcc
    43741 tctttttcat gtatttcatt aagacctagt gccttagcat ctttaagtac cattgcaatt
    43801 attcctctag ctgaacgttc tttaacagtt gtagctagct ccttaaatga tatgttgata
    43861 cttggtaatc ctatagccat actatcactc tcctaaattt aaattgattt ctttcatatt
    43921 ttcatatact acatcttctt catatacact atccagatag tttagtgtaa atgtaaagtt
    43981 caatacataa attgactctt cttttttaat actttgttct agctttgtta tatttaattt
    44041 tctgtctgtt acctttatat ttctgttaaa tagcttattt aattcatctg aaatttcata
    44101 cattctaata ctctttgact gtggttttgg taagtatttt atagagataa caatatcttt
    44161 gttattcgta tttaacatta caggtgtaca aataactgat agtatactta caaaaaaaca
    44221 agacccttca gagccttgca cactttcatc atcaataaaa atactttcag taaagttttt
    44281 tttgagaatc tcaataattg tttctaatat ttccttgtat ttaagcaaat aatcaccaca
    44341 ctatcactaa atattcttgc ttaatctgat aatcttacta taaatgatat aagtcatttt
    44401 taaatatata tctcatttat tcctgtcaag tatcttctat atttttaggt aatattattc
    44461 ttttcaatct atcacctcct tcaatatgga tttctttcac aataacatta tctcacatat
    44521 tttattgcct attgttgcaa tggtgtatat aaagtgttgt aaaattgtcc tattttttat
    44581 aatatctcac tcaatttaaa tatcatcttt ttctttaaat tgtagcaaga tgttctatct
    44641 aagtgtagtt tcatagaaat atatgtcata ttgttttttg tcttactatt ataaaaaaga
    44701 ttaaaaaatt ccatttctat atcatttaga cacgttagag cattttctat cttctctttt
    44761 tctatttcta tatcttcttt ttccattttt aattttctta atctttcctc tctttttaac
    44821 acctcatttt ccacagaaga gcttatgtta tatgtgttac tagtcttttc tgtatatacc
    44881 attgctccac atccagagta ttcattctca catcttttta tgtccaagtt aatattcttt
    44941 atctttatat ccaaatactt ataattatgt aatcttttat ctgtttcttt aaataatata
    45001 tcttttttgt tttccatagt acttcccctt ttttatatac ttattattag cttcaaattt
    45061 acaataatct ctttagtgca cagtttttta ctaatctcac aactttcaag aacatattgt
    45121 tctataactg tataaaaaaa acttcttcta tattttgttc taaaatacta gaaatcttct
    45181 tagccataag aagtccagga ttttttatac ctctttctat ttgagatata taatcatttg
    45241 taattccagc ctttctggca agttgaattt gtgtcatacc tttgcatttt ctataatatt
    45301 tcaagttatt tttaaacatt gttttatacc tccattacta ttaagaatat tttgttcttc
    45361 ctcacttaga ttatatagaa ttatttgttc tttatcaata gattttagaa aaaaatattc
    45421 ttttatattt tattagtttt attttttaga acaatttgtt ataatctata cataaggtgg
    45481 tgttataatg tttgccaaaa gattaagaga acttagaaag gaatttggat tgactcaaag
    45541 agaacttgga gaaaaagtag gcgtttccca aagagtctta gggtactatg aaacagaaaa
    45601 cagatttcct gatgaacaca ttttaaataa attggctgat gtatttaatg tatcagttga
    45661 ttaccttctt ggaagaacct tagttaagga aaatattgat acagtagctg cacatagaaa
    45721 aaatccacat gaagagttac ctgaagaagc acaagaacaa cttaatgatt atatagaatt
    45781 tttactaaat aaatataaaa aaaaataaac catatgatga gcagtctatt tttctgctct
    45841 tttaatataa tttaaaggca aacatacatt ctattttagg gggattttta tgaataattt
    45901 ggataagctt tttgaattag cttctcaaga agagataata attcattata ccacttatat
    45961 tgcaggtgat ttagaaggac tatatataaa caaacatggt attaaaatta tatcattact
    46021 cagtaattta aaacaaaact caaaaaaatt gacatccatc ttagctgaag aacttggaca
    46081 tcatttcact agccttggat actatgtatc ctcttataac gattattata caaaaattat
    46141 tatagataag tgcgaaaata aagccttaaa atgggcttgt gagttcttaa ttactgaaga
    46201 agatataata aacataatta actctggcat cacttgcgtt tatgaaatgg cagacatact
    46261 taatgtggat attactttct ttcaaaaaag attagaattt ctatcattaa aaaaacagtc
    46321 tttacaactt ggaaataata aatatttaat attaactaat ttgccgtatt tctacatatt
    46381 tgatccaatt tcataataaa aatagtagta attttaactg gcttaacagt aaaatctata
    46441 tatttatttt aacaaatttt ggtttctata aattcaatat taatatataa taatgatatc
    46501 atattattta aaattctaaa aacaaatata tgcttttgtt aagaaaggtt gtttgatgaa
    46561 tgtataaaga aaagttgttt gataattatt ttaagttctt agcactatta ttttggccaa
    46621 ttatgtggta taagtggata gtaatttcaa atggtactct tgaaaatatg ttatttacca
    46681 tatatgcaat cgtcgcaatt gttttcatca ttttatattc ggtgtttatg ataaaatata
    46741 aagacattac acaaatagat ttcttttaca ggataagtac tctacttgct tttatattta
    46801 cactttttag ctttttaata tacccaaaga gtttattttt cttgtattta aagattattt
    46861 ttactggaat atacctttat tattcaatag taaaaactct taaatttaaa gatgacgaag
    46921 gtgtagtggg tattatgagc tcacttttat taattgtaat aactttgttc tactaaattt
    46981 aattctattt gacttaatat ggctttaaat attagacatc tacattattt tctgatatta
    47041 tttaagatgg tagtcttata ttaataatga aatacactat gaaggagtta actattatgg
    47101 aaaaaaaagt tttagcaaca gttggagaaa aagagataac taatattgat attgaaaatg
    47161 cattaaaatc attagaccct tatcaagcta tgcaatttaa aactgaagaa ggaaaaaaac
    47221 acttattaaa tgatttagtg aatcaagaat tattcttctt agatgctaaa gaagaaaaat
    47281 tagatgaaga agaaatattt aaattagaaa tgaaaaagat agaagaaaat gtattaaagc
    47341 aatttgctat aaataaagtt ttatctagtg ttaatgtaac agaagatgaa aaagtaaaat
    47401 tctttgaagc taataagagc tctttctcta aaccagaaag tgcaactgct aaacatatat
    47461 tagtagatag tgatgaaaaa gctaaagaaa tacttgctca aataaaatca gaagaaatat
    47521 cttttgaaga tgcagctctt aaacattctt cttgcccatc taaagatatg ggtggtgatt
    47581 taggaacatt cggtagagga caaatggttc ctgaatttga agaagctgtg ttctctatgg
    47641 ctaaaggtga agtaagtgaa ccagttaaaa ctcaatttgg ataccatata ataaaattag
    47701 aagaccttca agaaagtaca gagtctacat ttgatgaagt taaggctgaa gttgaaaaaa
    47761 gtttattata tcaaaaacaa aatgaagttt atggaaataa gataaatgct cttaatgcta
    47821 agtacggaaa ccttgtaaaa tacaacgatt aatcataagg tcatatataa aaaagctgtc
    47881 ttttcacaat atttattaga aaagacagct ttttatatta aagtttaaat attcagtttc
    47941 atattatcat ttttttattg taaagaactc agcttaaaag tttaaataag atttttaatt
    48001 atcattagct aaatttttat atccacgtat tatctcataa cagtaaccta ttacatctct
    48061 tctttttatt atcccaataa atgtgtcttg gtcatctata acaggaatga aattttgatt
    48121 taaagatttt ataactaaat cttcaatatc tgcgttgatg gaaacaggag aattatccat
    48181 ttttctattt atatctgtca ctggaacatc ttccaatccc tttaagtcca gatttaaatc
    48241 attttttaat gtccataaaa aatcaccttc tgttatagtt ccaacatatt taccatcctt
    48301 acttattatt ggaattgcag agtatttgtg atattccatt ttttctaggg cttgccttat
    48361 agtgtagtct tcatatatat atgctacttc actttttggt gttagaaaga ataatatgtt
    48421 cattacacta cccaccgtta ctgaaaactt ataaatatta ctcaatattt ataatgttac
    48481 ttttttgcag taactttctc ctttcttatt ttctaagtat tcttaatact atacgctgta
    48541 acaagcgttt ttcctttgct cattttaatc ttattatgct ttattaatat ttttatatgg
    48601 tctataagta tcacttattc tctttttaga aaatttacta aaaattaatt gacttaaact
    48661 tagccataat aattttactc tacactatta attttaacat atttatctca ttattaatga
    48721 ttttatttta ataattttaa tttattttgc tattaatttt atttacaaaa actgaaaaaa
    48781 ccgctaaatg attagcggct tatctctcat attactagaa cactaatata tatgaatttt
    48841 taattataat ctaactacgt tagcagcttg aggaccttta gctccatcta ctacttcgaa
    48901 ctcaacgctt tgtccttctt ctaatgtttt gtatccatct ccagttatag ctgagaaatg
    48961 tacgaaaaca tctccttcac cttctacaga tatgaatcca aatccttttt cgttgttaaa
    49021 ccatttaact acaccagttt tcattaataa atcctcctaa aaaaacacaa atatttagct
    49081 actcttttct gtaaccttat tactactcta tcatatatag ctatgtttgt caattattat
    49141 tatttttata cattaataat caattttttt actttataat tttaaatgat tattatcttt
    49201 tacactttat ttttctttat aattttttaa taatttattt ttattatatg gaatcttatg
    49261 gacttatcac tccagcctaa atcccatggc accaacattc tatcagtatt atggcaagtt
    49321 accaaaggat atcctcttga gtctaatcct gtcactgtcg aaacatgtgt tattctgcct
    49381 ttcttctcat aaccaacaaa atcccctgga cgtaaattat aagcttcttt gtatacttct
    49441 tcataggaac ctttagcaat taatgaacct cttccactat acagtgcata atttttaaaa
    49501 gcttgtgcat ttacccatgc ttttgtacct gctctctcgc tataattcca aattgagttc
    49561 tttttgaacc ttccactttc aaacattatc tgtgatgcaa agtttgcaca atctccacct
    49621 tctggattat aatccctata gtttggatta aatttcatac catgttcttc atcagctgcg
    49681 gctccacagt atttatgtgc ataatctata gctttttttt gttcatctgt aagctgtaac
    49741 tctacaccat cttgttgacc aatataattt cttatatcat cagattttat attttctaaa
    49801 tttaaagagt ctgcaaaagg gtctgtatac cactctttag ttatgacata ttgattatct
    49861 ataattttta catttagata atgatatgta cctattctaa aggcattttc tttgtccttt
    49921 tgattttcat atgaatactt atattcagtg gatactgaac agattatacc atagaggtct
    49981 ttttcttttt cctttatttt tttaatctta acccttgtct ttatgtcttt aaattttaca
    50041 ccttgtttac tagaccagtt ttctaaatat ttcatctttt ttacttcatt ttcataagcc
    50101 cacagaccaa attttttatc cgtttcataa agacctttta aaatattttc atttttctct
    50161 aatattgcct tatttctgta gtcaaataag ttttcaagca atagtttata ttggtctttt
    50221 acatcatcac cttcaacatt tgcagattcc atgacttttt cttccatatt tttcatactt
    50281 aaatcattga ttaaaaatac acctgctgta gtagctaaag acaaaaatac aaatgccatt
    50341 atacctttga tgtacttatt cttatttttc agtattttca aagctacacc tcccaaatag
    50401 tacaccttac aaatcaaatt acaaagaata ttctgtcaca tatagtaatt ttcatattta
    50461 ataattctct attttattat gtccaatatt aaaaattaat tgtatcaata cactgttttt
    50521 tttacaattt tttattttat aatctacttt tttctttttt ttatagtaat atatatgtat
    50581 ataaatattt tggtatcatt ataattaata catactaatg gagggttttt tatgcaaaaa
    50641 aaagtcgcag caattaatga cttatctgga ataggaaaat gctcattatc ggttgctata
    50701 cctatacttt cagcattaaa ggttcaatgt tgtccatttc caacggccat actttcaagt
    50761 caaactggtt atcctgaatt tactttctta gactttactg atgaaatggt aaaatattca
    50821 aatgtttgga aaaatttaaa agtcaatttt gatagtatat atagtggttt tttgggttca
    50881 aagcatcaaa ttgagatagt ggctaatttt ataaacgatt atcccaatgc ttttatcgta
    50941 gttgaccctg tgatgggtga taatggagtt atgtatccaa tattcacaga agaaatgaga
    51001 caggaaatta aagaattagt taaacattca gatttaacta ctcctaattt aacagaagct
    51061 tgtttcttaa ctggaaatga ttataccaaa agcgattata atagagatga gcttatatat
    51121 attgcaaaat ctgtatctga tttgggacct agcaaagtcg ttattactgg tatattagaa
    51181 gatgataata tattaaattt ggcttacaat agagataatg accatgtatt ctttacatca
    51241 gtcaaatata ataattgttc ttacagtgga actggagata tattcacatc tatactttgt
    51301 ggtatgttag taaataaaca tgatttggga gtagcggtta atactgcaac agattttata
    51361 tataaaacta taaattacac atctcaattt gatactgata gaaatgatgg agttatgttt
    51421 gaaaactttt tatcagattt aaccaatata taaaatcaaa aaaactatcc attttataaa
    51481 tacctataaa tcataaaatt taggtattta caaaatggat attatttact aaactattgg
    51541 tatttcaatt gttaccatag caccaccagt tttcacagaa ttttcaatac ataaagtccc
    51601 attatgtttt tgtacaatat tatttacaat atagagtccc attccataat gagatttaga
    51661 atttctactc aaatctcctg tataaaactg ttctgtagct tttgacaaat ctgctttaga
    51721 aaaaccactt ccacaatctg taactacaaa ttttaagtat ttattatgta ctgatacact
    51781 tatgtatagt ttactctggc ttggtgaata atctatagca tttgaaatta tattcataat
    51841 agctctgaat aacagttctc tatctatagt tatattttta ggcaaattat cttgtttata
    51901 aaactccaca tctaagtttt tagtactagc taatgcttct atttgcacta aaacatcttt
    51961 tataaactca ctcatactga ttgcttcttt atttaatata tagccattct ctgtttttga
    52021 tatatctatt aaagtattaa tatatttttc tatttgagtg acaccctttg agatatattc
    52081 catgtattct aataactcag gattttcatt tatttcaatt aataagtcag tatttccatg
    52141 tataattgtt agtggtgttt ttatatcatg tgccagtgca gatatctgtt cctttctatt
    52201 ttcttctagc atccattgtt tttctaaaga atcttttaac gccaacttca ttttatccat
    52261 agaaaaaagg acattattaa tttcataaat tttactagat tctacaacaa aatctaaatc
    52321 ctgttgttct attttttctg tagtattttt taaaagctcc atttcaatat taaatttctt
    52381 gccaaatact tttgataata taacaatttc aataaaaaat attatactaa aaataatcat
    52441 acccaatgtc tctggttttg gtaaatatgc tcttaaaatt ggagaactat actctgatac
    52501 tattttgtat tttattataa aaacttcatc tttcttaaaa aactttatat aactttctga
    52561 atatgcatgc ctttcttcaa tatctctcat taaattccat acttcttttg attctttttt
    52621 attaaaggtc cctgaaatta aattcccatc caaagtataa accccatact catatatact
    52681 tggaattata tcttcagtta ctttttcact tgaaataatc tgttctttat atttattaaa
    52741 ttgtttttct gcataattag ctggtaaaat tacacctgat gataataata cagaaaatga
    52801 taacattaaa aggagtacaa ttgaaattgt cataataaag aataaagata aatattttat
    52861 aaaaatagtt cttaactttt caactttttt gttgttagat tttttacctc caagcatttg
    52921 taccctgtcc tccaatagtt taattaattc ataattaata cccaaccagt tttttatttg
    52981 attattttat atctttttat tgctaagtta tattttaaca taataatata aataaacagt
    53041 caatttttac tctattcttc tgatttttta ccttcccatt tagaaaacca aatacataaa
    53101 attacaaatg ctaaaattgt tacaaaacca caaattaaac ctgttgattg atatgctttt
    53161 attgattcaa cctttatata ttcaaccgtt ttagaacttg aaaaagacga ccaaatagtt
    53221 ataaatctaa caccccatgc acaaggaaaa tactgccaaa taccagaacc caatccagtt
    53281 aataataatg ctgaaagtaa catttcaact atacctactc caattgatgt tccttttccg
    53341 aaccttaaac ttaagaataa atgtaatatg tattcaaata aattacaccc aattaaaatt
    53401 agaattgaaa tcatataaaa gcttgatgta aagtaagcct tagatatacc aaatttaaaa
    53461 cctaatacag acactaatgt tgctacaaaa ccaagacata ctaagattat atatttactt
    53521 ataagagtaa gatattttgg attagatgtt gttaacatat gtttataatt ccctgcataa
    53581 tattcttgtt caacaaacat tgaacacaca acacttgaca ataaaggaaa agctattgct
    53641 ataacttgta aatatgctac tatctttagt atattatcag ctcctgaaac cttataatac
    53701 cataaaaaca atcccatccc cattaaagca atgcaaatgt gcattagtat tataaaattt
    53761 ctctttaatt ttattaaatc agaacaaaat attcttatta actgtctcat attacttcac
    53821 ctctctatta ttgaaccaaa cccttgtagc taaagcaaat aatacaaata aaacaataga
    53881 tattaatact cctggcaaaa taacagcatt atttaaaaat tcacttccct ctggtacagg
    53941 aagaccattt ggcagaatat gcaatacaac agccattaat cttgaaggaa tcgaatatgg
    54001 aaacatatac cacatttctg tagttgagaa aacagcaact ccaacaatag taaagaacat
    54061 atttgttaga actgtaacaa acataccaaa ttttgaagct aaaaacatac aaagaggaat
    54121 ctgccacata aatagtaaaa taattaatat acttgcaatg atattttgac ctattgaaat
    54181 agtttcttta tagattatcc caacaaaaac tacacctatt gcagaaataa ttgatgctat
    54241 aaaacaacaa ataatacagt aaatcatttt tccagtccaa acgtctttca tttcaattgg
    54301 caatggtaaa ataccacgat atttactttt ttcatctttt aatactatta aagaagatat
    54361 taatgttaat gctcctggaa gaaatgtcgt ataccaccag ttgaaagcag cattttgccc
    54421 tccacctagt ccacacaaaa gtatacaaaa cactggaact atccatatca acttcattcc
    54481 aaaagttcct ttcatctttt gaaattccac tctaataaat ctttccatat tactgccctc
    54541 tctttctatt ttcagctaca actttcataa aaagttcttc taagttttgt tcttttgata
    54601 cttctccttg ataacccatt actccattag aaattattcc tatttcatct acaacttgtt
    54661 caacttcaga caagatatgg cttgataaaa taactgtaat tccttgtttt ggaaaagaac
    54721 gaatcaattc tctaagttct tcaataccaa tagggtcaag accatttgta ggctcatcta
    54781 agattaaaag ttttgggtga tttaataatg caatagcaat accaaggcgt tgcttcatgc
    54841 ctagagagaa ttgacctgaa cgctttttaa aggtatctct taaatctaca gtctctaaaa
    54901 cttcttcaat gcgagagtca ggaagaccaa gtaaagtagt atggactttt aaattttctc
    54961 gtgctgttaa attctcatat atagcaggag actcaataag agaaccaata tttgataaat
    55021 cctttctagt ccatctatgt ccctcaaaca ttatttttcc tgaagttgga tttaaaatcc
    55081 cagttatcat ttttaataaa gtagatttcc cagctccatt tggtcctaat aatccatata
    55141 tagaattaga tttaattgct agtgaaatgt tatcaactgc catctgctcc ttaaattttt
    55201 tagaaactcc ttttacctgc aaaatataat tactcataat tttcatcctt tcttttgtca
    55261 aacttattta caagaatcat tctaaatcat aaatataagg attttataag aattaaaaaa
    55321 caaaaaaaga tgtgttaaaa acacatcttt ttttatctta attctattaa ttttccattg
    55381 tagtatatct ctacttcttc aatccaaatg ctattatctt cttcttggat gacattttta
    55441 ataatattat ttatagtata tattatattt tgttcattca aataatcatc tttccaagaa
    55501 actctacttt ttttatctgg ttctatcaag tttattttaa caagattatc tttttcctta
    55561 acttctactt ccatgtctaa accattaaaa tactcatttg ataatgtatc taagattaat
    55621 cgcagtttat ccttaagtga actttcttca tcaatctgta agtactttac tattttctca
    55681 tcctttgaaa ttatctctat aggtatttta gtagtttttt tagaattaat cattttacta
    55741 gttgtttctt cttttgcatc ttcatccttc tttacaactg ctgattcttt agaatcaact
    55801 agctcaactg cactgatgct ataatccata catgccgtac tcaataaagt agtagcaatt
    55861 attaaaaaac ttactgctat tttattcata agtatccctc tcctaattct ctttcgatac
    55921 ttattatacc atagtataga ttatattttt atacttttaa atcaatttat aaattattat
    55981 agtaaattat ttacaattct aagctatttg aatttatttt tatagtatat tctttctcta
    56041 aattcgacag atttttattc aaaagccctt ttatttccct cgtatttcta tacttgttgc
    56101 tatgttcttc attatgttca taatcatatg aatcattgtc atattcttca tcattattaa
    56161 attcttcaat tgcttctttt gactttatat cacaattaaa tttatactta tttactggta
    56221 tatcaagtct taccttagaa tttggaactt ctatgtccag gaacttagca attggcatat
    56281 cagagtacaa gtaaaattca ctttcataat ttgaatttct acctattttc tcttttatat
    56341 ttacattttc aggtatatac tcttctatat tattgataaa tacatctcca ttctctgaag
    56401 atatatccaa tgactctgat tctatgttta catttttaac gcctaaaatt gactttgttg
    56461 atagatgaag attgctatta gatattaagt ttaagttttc taatctattt actttatttt
    56521 ctgttatagc tctgctaaaa cctccagaag aagttttata tgttatatta tttgaatcta
    56581 catcatcaga tataaataag tacgaactta tacttgatgc atttatatct acattatttg
    56641 gtacatatat tctaatttct ctataatcac gtgaaaacat actatttata gatgtattta
    56701 ataagtggtc gaaacttcta atctttttaa ttttattttc agattttctt tcgcctttta
    56761 caactaaagt cttatcttgt agggttacac tgtatttgta aaattcatta ttccctcttg
    56821 ttgtcacacg cacatcttca tttgaacttt ttcttatcat aacatttata ttgtcaacag
    56881 ctatatctaa cttgtcaata tttacttttt cttgataaag tttgttttct ttgtttaatt
    56941 cagcttcctt ttctaaacca aagtttatta ggcttggcat aactaaaatc cctgaccatg
    57001 ttgtccctat tatacctatt accaataaaa ctgcagcaaa tatagcaagt tttttattca
    57061 tgtttatctt ctcctttctt attttctata tcttcaaggt ccttatatat cgcctcaaaa
    57121 tttatactat catcattata ttcatcagtt tcttttatgc tttcttcttc tctattgtag
    57181 taatcattat tatatgaatt ttcattaatg tctttattca aatcttcatc atttaaagta
    57241 gctttttttc ttttttccat cttttcattt gcctttatat aaatattttt tctctttatc
    57301 caatgtatat aattatttag taattgttta cctagtttta tggcaaataa taaaatttgc
    57361 catgcaagta tctcaagccc tacaaatgct atggttgcga aaatacaaaa catcataaca
    57421 tctggaacca ttgctataaa tttaatcaca aatggaatta atataacaaa agcaattaat
    57481 gagcatacta atgcaatagt agcagataca atagcacata caaacgcaaa tgctggaaca
    57541 attagaaata aagataacac tactaataac ccctttataa cttgtctgga taatcctcct
    57601 acaccttctt ctatacttgg tgtaagttta tcatttatat tttcttttga tttattgtac
    57661 ttttccttag cttcatcttt caagatagaa taatcctttt ttacatttga tttaaatcta
    57721 ctaaatttac catttaatat gtctgtattc attatttttc catctatatt ttcttttgat
    57781 ttactatact tttcttttgc ttcatctttt aagatagagt aacctttttt catatctgat
    57841 ttaaatttac taaacttatc attcaaaaca tctgtattta ttattttttc ctcttcaata
    57901 tttttttctt tagtttgtaa gataagttct tttgctatag attttggtga gccaagtgat
    57961 gctattatct ccatatcact ctttccctct ataagaccat ctacgaaata ctcttcataa
    58021 tctctcaata tatcattaac ttcgctctca gaaaaatctt tttttagata atcctttaaa
    58081 atttcaagaa actcagtttt attcaagttc attacctccc tcatcttcac ttattaaaat
    58141 ctcaacagcc tttacaaatt ctctccactc ttccatcaga ttggataaat tctcttttcc
    58201 tagtgaagtt attctatagt attttctagc aggaccttca ttagattcta gtatatatgt
    58261 ttcgaaatac aaatcttttg taagtcttct tagaatagga tatattgttc cttcatttac
    58321 atctattact ttagagatgt tatgtactat ctcatatcca tacatatctt tcttagatat
    58381 gagagcaagt acacaaattt ctaaaacacc ctttctaaat tgtgtgttca tatactccat
    58441 cctttcttta aatacacagt attgtgtttt acaaggtact atgttaaaac ttcttatatc
    58501 ctcataataa cacagtacta tgttctgcac aatagttatt tttaaatatt cttgttgatt
    58561 aaatccttct ataaatgtat aatactctaa tcagatatat tttatgaaag tggtgataaa
    58621 ttggctaaaa tgaaagatga taaaacaata ttaatcctaa ctgctcaatt tggtgctgga
    58681 catataagtg ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag taactataat
    58741 gttgttatac aaaactttat aaatgcaagt attcccatga tgaataagcc tatggtaaaa
    58801 ctttatgaaa acaatacaaa atatactcct ggattatata attactatta ttactttaag
    58861 aaatcgtttg attcaagaca tgatttatct cataaattat atacacctaa actttctgag
    58921 tatattgctg atataaatcc agatttgatt atctctacat ttccactagc tgctgcttgt
    58981 gtaaataact ttaagataaa aaatcctgat ataaatatac ctacacttac agttataaca
    59041 gatgttgtag atagtatgga atgggttttt gaaaatacag atttgtattt tgttccttct
    59101 cctgaaataa aaaatagatt ttttcagaaa ggaataaatc cagactccat aaaggttact
    59161 ggagttcctg ttgataagag atttcaaatt gaaagtaaag aactatgttg tgataaatat
    59221 agactattac ttctaggtgg aggaagaggt ctatttgata tagatgagga ttttatgcat
    59281 tggatagatg aatttattga agaacatagt aattctatag aaattacaat agttactggt
    59341 aaaaacaaaa aattatatga caatctaact cataaaaaac ctctaaaaaa tattaaagta
    59401 cttggatttg taaacgacat gtataatcta ataagagaat gtgacctaat gctaactaag
    59461 cctggaggag caactatatt tgaagctatt caatcacaaa ctccagtact cgtaaaaatg
    59521 ccaaaagttg gacaagaaat tgaaaacgct aaatttataa tagataaggg tcttggcatg
    59581 atttatagtg atgacctaga ccttaaaaac atattttata aattagtatc aaatgaattt
    59641 gattctataa taaactttat gaaaaaaaat ttaaaagaat ttaaaacagt cattcatcca
    59701 gagaaaattg ctgattatat atcagaatta atagataatc attacagtta aattgtacta
    59761 ttaaaaaatt tcaaacataa aattaaaggc aatcaagtat gcttttacac ttgagaattt
    59821 actcatagtg tttaataata cttgattgcc tttatcatat caaaatcaaa ttgttatatt
    59881 agtatatatt tttcttgtac tgcatataca gttgttgtat ttcttccata cttctcttta
    59941 attctatttg taaatctgaa gcagtctggt aatccttatt ttctatagct tctctcactc
    60001 tagtgaatag tgattctcta gttaaagtat ccttcataat attgaacttt aaatccgata
    60061 ttgagttcca aactacctca tcaagcgcat ccatattttc ttttgtgtgt atcttgacaa
    60121 tacttcttat tttttgaatc ccagcctcta ttattctagt ttttaatttc ttttgccact
    60181 tatctatagc tccaatctta aatgattcta ttatactatc agtaaataca tcaccttgtt
    60241 ttaaagattt taattttgaa gcatatattt ctaagttttt catattttca taaactgtag
    60301 ccggtggtat tccaaatctt gcatttcttt cttccaaact ataatgttca aatacatcat
    60361 tttcatctct atatgctcta tctttttcta aatagaaact ttcttcacct acatttttag
    60421 ataactcttt ttcaagttct tttgtactaa gaccactctt agctgctgct tttataccat
    60481 ccaacattgt ttgataacat cctgctatta ctaaatacgt gttagataga gggcttggtg
    60541 accttaactc aaatcttaca gtctttggat ttttcatatc tcttattaat ccaacaagca
    60601 ctgacctatt tcttgatgga atctcataac tatgacctaa agatgtaact atgcatacag
    60661 gagcttcaaa tccaggaact aatctattaa atccatcatt agaagctgtt acaattgggt
    60721 ttaatacttc ataattgtaa agaagtccca taagagcccc atatcctagt tcacttagat
    60781 aatcttcttt taaatctttt ggcgcaaata gattttttat acttccatcc tttaacttcg
    60841 cacttactcc tacatgtgta tgtccaccac ttcctgctac tccatgaatt ggttttgctt
    60901 taaatgtaac ttctagtcca tgtgatgtaa atacatcctc tataacatct cttactaata
    60961 attcattgtc tgctgcttgt aacggtgtag aaaacttcca agaaacctct aattgctcca
    61021 tagcatggtt tgttcttcca tctatactaa tagaactttg tattcctccg acctctttgt
    61081 gagccatttc agggtttaca cctagctttt gaagtataat taaacttttc tctaaacaag
    61141 ttcttataat accatgagtt cttttccagt attgctcttt taggctttgt gatacatata
    61201 atttctctaa gtcagcttta tcctcaggag tattaaccca gaactcaagt tctgtagctg
    61261 ctgttaacat tatttcctct atatcatcta ctgaatctat tcctatgtta tttattacat
    61321 gtggatattc tctaaatatt tcatacattg actttttaaa gtacttatct gctttttgaa
    61381 gtactcctct agaacatact tttttattat catgtattaa aaatgcaggt atctttaatg
    61441 ttccaacagg tagacctact tcttcgtcta tatattccat attgtaatct acataccaat
    61501 gacaactttt atctggcatt aaatctactt tcgcattatt taatgtagct atattataaa
    61561 gctcaacact tgaaccatca gtctgtatag ctgattccaa aaatttatct atatcctcca
    61621 aaaataattc tacaggaatc ttttcatctg ttgcatttcc tcctaaatca acccccatta
    61681 aggaaacaaa tcttatattt ttattgcttt ttaggatttc tcttaattct tcattagtgt
    61741 gtttatcttt ctctattacg tataatagac tatccatttt tccactctcc cataattttt
    61801 atgtatattt tattttgtta aatacaagat aaatttcatt ttccttacat ttatgaattt
    61861 ttcttgcaaa ttattcgtgt tttattcgtg tttttattta tattattata tatcaagtat
    61921 aaaaagttcg caatattttt tattggaaaa aatattatac atacaatagt ttaacatttt
    61981 tacaaatatg gggtatacta aaataaaagt atttttattt gaaaatttaa ttttatttat
    62041 aatataatct atagtaaaag aaataataaa aaaatttagg agataaactt tatggaatac
    62101 ataattttcg atttggaatt taaccaagga tttgataaaa aactcaataa aactgtatcc
    62161 aatgaaaagt gtccttttga aattatacaa ataggagcaa ttaaattgga ttctaaattc
    62221 aatataatag atacgtttaa tagctatgta aaacctacaa tttataaaga catacatccc
    62281 tttattagtc gtatgactaa tattaaaagt tctgatttta atgattcgcc tactttccct
    62341 gaggtctaca acaattttat aaaatttata tcatcacaag accctatatt atgtgtttgg
    62401 ggagctggag atttgaagga attatacagg aatatcaact atcacaagct accatcaaat
    62461 tccttaccta aatcttatat taatatacaa caacatgcat ctaaatattt caataatcca
    62521 gcaggaaaaa gcattggcct acaaaatgct atttctatat tggaacttga tgaaaaaatg
    62581 tcttatcaca atgctttaaa tgatgcatat tatactgcta aagtttttat taagatatac
    62641 aatccaagta tagtcccaga tatatatctt tatacgagta taaaacctaa aactataaga
    62701 tattctaaca aaaaaagagt tgattatgat aagttatttg atgaatttag gaaaatttta
    62761 aatcgtgaat taactaaaga cgagaaaaaa atcattaact tagcttataa tatgtggaaa
    62821 acaaatcaat ttacattaga aaatgttaag caaagaaaaa acaaatagag tttatttaga
    62881 taaaaaatag gcatgtagat taaaaaacta gttaacttta aatttatatc tacagcccaa
    62941 tttttatcat aattaatttt tattttatct ctaattcaac tggacaatgg tctgaaccta
    63001 atatttcagt gtgtatatcc gcacttacta atctatcttc taattttttg gatgcacaaa
    63061 aataatctat tctccaccct gcattatttt ttcttgcatt aaatctatac gaccaccaac
    63121 tataaacacc ttctttatca ggattaaaat atctatatgt gtctataaac ccactgtcta
    63181 ataatttaga aaacttctct ctttcttctt tagtaaaacc tgcatttttc atattatttt
    63241 taggattttt taaatctatt tctttatgtg caacgttcat atctccacac acaattacag
    63301 gcttatgatt atctagtttg gttaaataat ctataaagtc gtcttcccat ctagttctat
    63361 attctagtct ctttaattca gattgagaat ttggcgtata tactgtaaca aagtaaaaat
    63421 cttcaaactc tagagttatc actctaccct ctttgtcatg ttcttctata tttattccat
    63481 acattacttt taaaggctct ttttttgtaa atatagcagt acctgaataa ccctttctct
    63541 cagcataatt ccaatattga aaatatcctg gtaaatctaa ctctatttgc ccttcttgca
    63601 atttagtttc ttgtaaacaa aatatatctg catctacttc tttgaaaaag tctaaaaatc
    63661 ctttcccaac acaagctctt attccattaa cattccatga tataaatttc atacttatac
    63721 tcctcatcta aacattgaca cactccccat agctaaagat gggggctttt caacaatttt
    63781 attgtaaatt cttaatttac ttttatttta acaatgctat actataattt catctaaaat
    63841 tactatttac ttaataccta aaaatatatg ttttaatcaa tttttaactt ttttgttaaa
    63901 tcttcagggc tcattgaaga aacatacata tttgttccaa gttcaaatcc tccaaaatta
    63961 tattttcttg ggattatgtg catatgaaca ttaaaatacg attttgtttc aatctttgtt
    64021 ggatgagtgt gaatacataa attaaatggc atatatccat tgatattgta taacttctta
    64081 aacaactttt caaatattgt agaaagctcc tgtatattat tatcatttaa ctcaccaaac
    64141 tctattttat ctttaaatat tattcttact tctccactat acttggttgc atatggtata
    64201 agtaccaaaa aagcttctcc attataaaca actcttctat ctaaacttat ttcattttct
    64261 attaaatcat catacaaaga gcttttattg ctgtcataat actcctttga aaccaacagt
    64321 tcattcgtaa tttcaggtgg aataaaagac atagacaata tttgagcatg tggatgcatc
    64381 aaagatgctc ctgcctttct caagaagttt ttgaagatac taacatactc tactttatca
    64441 tctttcgaca aatctctaaa tctatttcta tacataaaaa aaacatcttc aaattctttt
    64501 tgactcatgt tataaaaact acgattatgt ttatcgctct ctatcattac ttcatgaaca
    64561 ccatatacat ctcttgccat atcatcaatt ataggaaatt tattgtagac agattttaca
    64621 agccatcctt cttcactatc tatttcaaat aatgcatcag ttgcatgagc ttcatttcct
    64681 ttgcaaaatg ggcattcttc atcatattca ttagtttctt catcttcatt tgggatatct
    64741 gctgtatcta aaggtctttt taacctatct gtagcaaata taacaacatc attggtaatt
    64801 ggatctattc ttaattcttt cataattaac acctcttagt taatagcctt taatataatt
    64861 atatgatatt tatcgtatct gttaaataaa tttataatat tttttataat tttgcatatt
    64921 tattattata tattttgtct atagctga
//
LOCUS       DAELNY010000003        64158 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.003, whole genome shotgun sequence.
ACCESSION   DAELNY010000003 DAELNY010000000
VERSION     DAELNY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.003"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(275..2245)
                     /gene="ftsH"
                     /locus_tag="KPE55_000142"
     CDS             complement(275..2245)
                     /gene="ftsH"
                     /locus_tag="KPE55_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0491248.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2279..3667)
                     /gene="tilS"
                     /locus_tag="KPE55_000143"
     CDS             complement(2279..3667)
                     /gene="tilS"
                     /locus_tag="KPE55_000143"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG0491249.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3850..4278)
                     /locus_tag="KPE55_000144"
     CDS             complement(3850..4278)
                     /locus_tag="KPE55_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG0491250.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4295..5101)
                     /locus_tag="KPE55_000145"
     CDS             complement(4295..5101)
                     /locus_tag="KPE55_000145"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG0491251.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5303..5788)
                     /locus_tag="KPE55_000146"
     CDS             complement(5303..5788)
                     /locus_tag="KPE55_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG0491252.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5839..6498)
                     /gene="spoIIR"
                     /locus_tag="KPE55_000147"
     CDS             complement(5839..6498)
                     /gene="spoIIR"
                     /locus_tag="KPE55_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG0491253.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6643..7323)
                     /locus_tag="KPE55_000148"
     CDS             complement(6643..7323)
                     /locus_tag="KPE55_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0491254.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7307..8197)
                     /locus_tag="KPE55_000149"
     CDS             complement(7307..8197)
                     /locus_tag="KPE55_000149"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG0491255.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTKIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8315..9865)
                     /locus_tag="KPE55_000150"
     CDS             complement(8315..9865)
                     /locus_tag="KPE55_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG0491256.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9959..10225)
                     /locus_tag="KPE55_000151"
     CDS             complement(9959..10225)
                     /locus_tag="KPE55_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG0491257.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10373..11233)
                     /gene="yabG"
                     /locus_tag="KPE55_000152"
     CDS             complement(10373..11233)
                     /gene="yabG"
                     /locus_tag="KPE55_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG0491258.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11317..12255)
                     /locus_tag="KPE55_000153"
     CDS             complement(11317..12255)
                     /locus_tag="KPE55_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG0491259.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12365..12526)
                     /locus_tag="KPE55_000154"
     CDS             complement(12365..12526)
                     /locus_tag="KPE55_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491260.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12536..13033)
                     /locus_tag="KPE55_000155"
     CDS             complement(12536..13033)
                     /locus_tag="KPE55_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0491261.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13148..13858)
                     /locus_tag="KPE55_000156"
     CDS             complement(13148..13858)
                     /locus_tag="KPE55_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG0491262.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(14034..14564)
                     /locus_tag="KPE55_000157"
     CDS             complement(14034..14564)
                     /locus_tag="KPE55_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491263.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNYINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14581..14811)
                     /locus_tag="KPE55_000158"
     CDS             complement(14581..14811)
                     /locus_tag="KPE55_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491264.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14826..16220)
                     /locus_tag="KPE55_000159"
     CDS             complement(14826..16220)
                     /locus_tag="KPE55_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0491265.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16346..17599)
                     /locus_tag="KPE55_000160"
     CDS             complement(16346..17599)
                     /locus_tag="KPE55_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0491266.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17650..18804)
                     /locus_tag="KPE55_000161"
     CDS             complement(17650..18804)
                     /locus_tag="KPE55_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0491267.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19364..19933)
                     /locus_tag="KPE55_000162"
     CDS             complement(19364..19933)
                     /locus_tag="KPE55_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0491268.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20136..20363
                     /locus_tag="KPE55_000163"
     CDS             20136..20363
                     /locus_tag="KPE55_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0491269.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20582..21325)
                     /locus_tag="KPE55_000164"
     CDS             complement(20582..21325)
                     /locus_tag="KPE55_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0491270.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21493..22365
                     /locus_tag="KPE55_000165"
     CDS             21493..22365
                     /locus_tag="KPE55_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0491271.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22365..23030
                     /locus_tag="KPE55_000166"
     CDS             22365..23030
                     /locus_tag="KPE55_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0491272.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            23051..23908
                     /locus_tag="KPE55_000167"
     CDS             23051..23908
                     /locus_tag="KPE55_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0491273.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(24064..26610)
                     /locus_tag="KPE55_000168"
     CDS             complement(24064..26610)
                     /locus_tag="KPE55_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0491274.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26610..27296)
                     /locus_tag="KPE55_000169"
     CDS             complement(26610..27296)
                     /locus_tag="KPE55_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0491275.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27379..28368)
                     /locus_tag="KPE55_000170"
     CDS             complement(27379..28368)
                     /locus_tag="KPE55_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0491276.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28355..29041)
                     /locus_tag="KPE55_000171"
     CDS             complement(28355..29041)
                     /locus_tag="KPE55_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491277.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29541..32747)
                     /gene="carB"
                     /locus_tag="KPE55_000172"
     CDS             complement(29541..32747)
                     /gene="carB"
                     /locus_tag="KPE55_000172"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0491278.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(32940..33989)
                     /gene="carA"
                     /locus_tag="KPE55_000173"
     CDS             complement(32940..33989)
                     /gene="carA"
                     /locus_tag="KPE55_000173"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0491279.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(34246..37452)
                     /gene="carB"
                     /locus_tag="KPE55_000174"
     CDS             complement(34246..37452)
                     /gene="carB"
                     /locus_tag="KPE55_000174"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0491280.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(37505..38548)
                     /gene="carA"
                     /locus_tag="KPE55_000175"
     CDS             complement(37505..38548)
                     /gene="carA"
                     /locus_tag="KPE55_000175"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0491281.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(38574..39290)
                     /gene="pyrF"
                     /locus_tag="KPE55_000176"
     CDS             complement(38574..39290)
                     /gene="pyrF"
                     /locus_tag="KPE55_000176"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG0491282.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(39666..40301)
                     /locus_tag="KPE55_000177"
     CDS             complement(39666..40301)
                     /locus_tag="KPE55_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG0491283.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(40325..41239)
                     /locus_tag="KPE55_000178"
     CDS             complement(40325..41239)
                     /locus_tag="KPE55_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491284.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            41840..43069
                     /locus_tag="KPE55_000179"
     CDS             41840..43069
                     /locus_tag="KPE55_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0491285.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(43274..45655)
                     /locus_tag="KPE55_000180"
     CDS             complement(43274..45655)
                     /locus_tag="KPE55_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG0491286.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(45627..46352)
                     /locus_tag="KPE55_000181"
     CDS             complement(45627..46352)
                     /locus_tag="KPE55_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG0491287.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(46342..46797)
                     /locus_tag="KPE55_000182"
     CDS             complement(46342..46797)
                     /locus_tag="KPE55_000182"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG0491288.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(47079..48260)
                     /locus_tag="KPE55_000183"
     CDS             complement(47079..48260)
                     /locus_tag="KPE55_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0491289.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(48250..48942)
                     /locus_tag="KPE55_000184"
     CDS             complement(48250..48942)
                     /locus_tag="KPE55_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491290.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(49076..49792)
                     /locus_tag="KPE55_000185"
     CDS             complement(49076..49792)
                     /locus_tag="KPE55_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0491291.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            49917..50651
                     /locus_tag="KPE55_000186"
     CDS             49917..50651
                     /locus_tag="KPE55_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0491292.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(50936..51691)
                     /locus_tag="KPE55_000187"
     CDS             complement(50936..51691)
                     /locus_tag="KPE55_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0491293.1"
                     /translation="MKKYNISENGALIKNDTIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRHIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(51813..52751)
                     /locus_tag="KPE55_000188"
     CDS             complement(51813..52751)
                     /locus_tag="KPE55_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0491294.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(52854..53504)
                     /locus_tag="KPE55_000189"
     CDS             complement(52854..53504)
                     /locus_tag="KPE55_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0491295.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            complement(53653..53841)
                     /locus_tag="KPE55_000190"
     CDS             complement(53653..53841)
                     /locus_tag="KPE55_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0491296.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPEDLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(53948..55132)
                     /locus_tag="KPE55_000191"
     CDS             complement(53948..55132)
                     /locus_tag="KPE55_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0491297.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKINLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(55274..56191)
                     /locus_tag="KPE55_000192"
     CDS             complement(55274..56191)
                     /locus_tag="KPE55_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG0491298.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(56213..56941)
                     /locus_tag="KPE55_000193"
     CDS             complement(56213..56941)
                     /locus_tag="KPE55_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0491299.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(57137..57337)
                     /locus_tag="KPE55_000194"
     CDS             complement(57137..57337)
                     /locus_tag="KPE55_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491300.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(57462..57842)
                     /locus_tag="KPE55_000195"
     CDS             complement(57462..57842)
                     /locus_tag="KPE55_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0491301.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(58115..59473)
                     /locus_tag="KPE55_000196"
     CDS             complement(58115..59473)
                     /locus_tag="KPE55_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0491302.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGALYCLRLRGTKWLKHK
                     VA"
     gene            59921..60373
                     /locus_tag="KPE55_000197"
     CDS             59921..60373
                     /locus_tag="KPE55_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG0491303.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(60530..61147)
                     /locus_tag="KPE55_000198"
     CDS             complement(60530..61147)
                     /locus_tag="KPE55_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG0491304.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(61570..62382)
                     /locus_tag="KPE55_000199"
     CDS             complement(61570..62382)
                     /locus_tag="KPE55_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0491305.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(62458..63876)
                     /locus_tag="KPE55_000200"
     CDS             complement(62458..63876)
                     /locus_tag="KPE55_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG0491306.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT        20419 a        11614 c         6970 g        25155 t
ORIGIN      
        1 ttctaataaa taattataca ttattaatat acttttaata ctattaattt taaatttcct
       61 ttttaattat tataatggtt gttttatatt ttatattgat gatttatata attttacatt
      121 ttatttaaat aattttaaaa taataaaaaa actgaaagta tcaattaaat taatacatgc
      181 aaatataatt tgcttattaa tttaatatta tactttcagt tttagtattt aatccacatc
      241 agataataat cttaaaatga ccaaggtctt aaaactattt atctttatca gatttatctt
      301 ctaaattttt atttatatca ataacattgt ttctttcttc ttcatctttt tctaatttta
      361 tagttaattg ttcttcaact tctttagaag aattttcctc tgtaactgca ttttcaattt
      421 cattcagagg taactcttta ttaaatgctt ttacaaattg ttctgcatct aaagtttcat
      481 atataagtaa agcttgtgct acatactcta atctatccat attttcttgt aatatacttc
      541 ttgtcttatc atatgcacca tcaactatac gctttgtttc ttggtctatc tcaaaagcaa
      601 cttcttcaga ataatttctc ttacttgaaa aactattacc taggaatact tcatcatcac
      661 tgtcaaatga cattggacca agtttagaac tcattccata ctttgttacc atacttcttg
      721 cagtagctgt aactctttct aagtcatttg aagcacctgt agatacatcc ttaagtacta
      781 gctcttctgc tactctacca ccaagtaaaa ctacaatatt ttctttcatt tcattttttg
      841 tagcatagaa cttatcttca acaggaagtt gcattgtaaa ccctccagct cttcctcttg
      901 gaactattgt aacttgatga acaggactta cttcttcaag tacatgggca caaactgcat
      961 gccctccttc atggtatgct gtaagtcttc tttctttctc acttattact ttcgattttt
     1021 tagccacacc tgctataacc tttgttatag cttcttctat agtttccatc ttaattttct
     1081 tttctctttt tctagctgtt aatattgcag cctcattcat taagttctct atatcagcag
     1141 gagtaaatcc tggtgttctt ctagccaaga catccatttt tacatcatca cttaatggtt
     1201 tatttcttga gtgaacttta aatatagcct ctctaccttt tacatctggt gtaccaacaa
     1261 caacttgtct gtcaaatctt cctggtctaa gtaaagctgg gtcaagtata tctggtctat
     1321 ttgtagctgc catgattatt ataccttgat ttaccccaaa tccatccatt tcaactagaa
     1381 gttgattaag agtttgctct ctttcatcat gacctccacc aagacctgca cctctttttc
     1441 taccaactgc atcaatttca tctatgaata tgatagctgg agcacttttc ttagcctgtt
     1501 caaataaatc tcttactctt gaagcaccaa ccccaacaaa catctcaacg aaatcagaac
     1561 cacttatact gaaaaaggga actcctgctt ctcctgcaac tgctcttgat agatatgttt
     1621 taccagttcc aggaggtcct accataagca ttccttttgg tattcttgca cccaattcaa
     1681 tatatttctt aggattcttt aagaaatcta ctacctcctg caaatcttct ttttcctcat
     1741 ctagtccagc tacatcttta aatgtaactc tagttttttc atcatcttta tgaaccttag
     1801 cttttgattt gccaaaactc ataacctttc cgcctccacc ttgagactga ttcataaaca
     1861 cgaaccaaat aatcaccatg aagaatatta acagtaatgt aggcaacatt tcgacaaacc
     1921 aaggtgtaga tggcttagct tcaccaccaa aagttagttt cccagcctta gcttggtcta
     1981 acacctcatc tgcaagttta tttcccatca cttctgttgg aacatatgac ttaaactttg
     2041 tatttgtatc ttttatagta ccttctacag aagtttgatt tacaaagtat aatcttgata
     2101 tattctcatc tgttaattct ctatatactt cagagaattt taaatctttt attttttctg
     2161 taggcttacc tgagaattga actataccta ctataattat aaaaacaagt agataaaaac
     2221 ctgccccttt tagcaactta ttcaatatag aagccctcct ttcatacatt gctttgccct
     2281 ataatttgtt aaaattaatt tttaatactt cttttgtgtt ttcatcaatt ttataatttt
     2341 cactgatttt ataatctcca acacatatta ttccttcact atctgtgatt ataggaattt
     2401 tacatctttc ttctcttggg atttttaaat ctatgaataa atctttcact tttttacttc
     2461 ccatagcaag ctttatctta tcaccttgcc ttctgcttct tattactata ccccctttta
     2521 ccttattaaa atcaaaccct ttagatgaat tgtctaattt catactctta tacctatcta
     2581 tgctcattac ttgggtctca ataatttgtt ttaattcttt tattttaata aacccattac
     2641 taggtacatt gtaataaaat tcaatttcct cattaacaat ttcctcattc gttaatatta
     2701 tactgtcttt ttttctataa acaaatatat ttcttggaag agttaacatt ttatttactt
     2761 ttgaattatc ttctaaagac attatatctt ctatatgtct ttggtcaaca aaattagtat
     2821 ctcctagtat atgttttatg gaatttctaa gaactctaac tttgatagca tcatgtaagc
     2881 aaacaaaatc gtctaaattt atctctacaa agcctttttc ttttatattt gaaacttctc
     2941 taaatttagc ttctgcctct ttctcaatat aatcgttatc actttttaaa ctattgctca
     3001 tcctcactat agattctatt acatttgaat taaaattatc cttcatatat ggtaaaaggt
     3061 ctaaccttat cttatttcta gtgtatatat tttctaaatt tgttttatct atcctcggat
     3121 ttaaattata agcttcacaa tactcctcta tctcatttct ttccacgtct aatatgggtc
     3181 taattatgca attatctcta atataatcaa ttccctttaa accttttaaa cctgttcctc
     3241 tcatgatacg catcataacc gtttcggctt ggtcattgag attatgacct attgctattt
     3301 tattggcttt aatcttatcc ttaatttcat aaaacatttc atatcttagc ttccttgctc
     3361 cttcttctaa tgataatccc tcattttcac aatattttgg tacattaata gactttacaa
     3421 agaatataat tcccatatcc tcacaaagtt tagatacata taatgcatct ttttgagctt
     3481 ctatccctct tatttgatgg tttaaatgtg ctgcatatat ttctatatta aaatcttttt
     3541 ttaatctatt tagtacatgc aataaacaaa ctgaatcagg tcctccagaa agacctaaaa
     3601 ctattttatc gcctttttgt attaaattat gtttatttat agtacttagt accttatcaa
     3661 atatcatatt tatccctctt acacttagtt ttattcactt ttaagtatac tttctataaa
     3721 aaaacgctaa acccttgtat caattttaag aaattaaagt ttaatataaa tataataata
     3781 ccaaataaat taaataaaag gtagtttttt cactaccttt ataaaatttc tggaaaatag
     3841 ccatagttat tatttagtac ctatgtatat atctatttta ttcctaccta aaaataaatc
     3901 ttgtaccttt atattatagt ctattttcaa ttttatgtgt tccccttctt gtatatttat
     3961 atctaattct cttgtatcaa tatcaattaa taaagtacct tgtggggtca catactttgt
     4021 agtactactt tctccacatt taaacagcat tgttgagtta atagcaccaa actttttgat
     4081 actaacttga tttttgagta tctttatagt attagttatt tctatatctt cttctttttc
     4141 cttgtaaata acatatattc catcattttt ataaaaaatt ttaccaaagg cagtcatctc
     4201 tatggtgtcc atatttcctt tttcatcata ttgtctagta tttatactta tttttacact
     4261 caacgatttt tcctccatag attcttcata taaactagta tttttgtatt tctacttttt
     4321 gaatcttatc aatttttttc tttaagaact ctcttgctat atctgaaaat ttttcaggta
     4381 tatctgatac ataatattga taagttccat gaatttctgt attgtttata atattttgca
     4441 cctctaaaat cttttttaaa tcctttgcag tctctttagc tggattaaca agttttatat
     4501 gctctccaac ttcttcacct attgttctct ttaaaatagg ataatgagta catcctaaca
     4561 ctaaagaatc tatatttttt tctttcaatt cttgtaaata tatctttgct gtcaatttag
     4621 ccacttctgt atttgcccat ccttcttcaa caataggcac aaataaagga catgctttat
     4681 taacaatttc tatactctca tctattttag atatttccaa gttgtatgct tttgaactta
     4741 tagttccttc agttcctatt attcctacaa ttttgttttt tgtagaactt acagctgttc
     4801 tagctcctgc ttctataact ccaattatcg gtatatcata tttttcttga gcttctttta
     4861 agcttcttgc agtagcagtg ttacatgcta taactatagc ttttacacct tttgttttta
     4921 aaaagtttat agcttgaaaa gtatatttaa ttatagtttc tttggatctt gaaccatatg
     4981 gtattctggc agtatctcca aagtatatta tatcctcatt tggtaatatc ttcattatct
     5041 cttttagaac tgttaatcca cctaatcccg aatcaaaaac tcctattggt ttattgttca
     5101 tctttataca acacctactt tacatttttg tgtttaacaa caacttattt atcgctaaaa
     5161 tttatattta tattcataat taatatatta ttacctaaat gattattata gtttatttta
     5221 cacaaaaaat aaaggagcta tcttaaatta gaaaataatt tctacaaaaa gacaactcct
     5281 ttcactactt aaaaatttaa atttattttg caaacacatg atttcctatt ctcatataag
     5341 gattcaatct atttatccat ttgcaagtgg ctatttctgg attccaaaaa tatactgctt
     5401 tattagttgg gtcagttcca taaagagctt cctgagctgc tttacgtgct gagtctgttg
     5461 gtgccatctt tattgaacca tcaagcacaa ctgagaaagc atttttttga tatatgactt
     5521 cttctattgt gtctggaaat ctagggtcca atactctatt tattactaca gctgctacag
     5581 caacctgtcc ctcatagctt tctcctctag cttctcctgc aaccaactta gataataaca
     5641 tcaagtcatt atcagataaa tttattactt ctttctttgc tatatttttt tcttgtttgt
     5701 ttactttctt aatattttct gttggttcat atgcaaatac ctctttaata ggcataaata
     5761 taaaagtcaa agctaatagt gtagccatca gtttactcca cctgtccatg attaaaacca
     5821 ctccttacat tcttattttt aaaatttttt ctctaaatcc ttgactactt caagtacttt
     5881 aaacttaatt tttactctac ttgttttttt tggtgtatct tgttttatta acttatattc
     5941 tttatcagtt aaaacttcct tcaacttatc atctgtagat ttatctatca ctccattaga
     6001 ttcatcaaca aaacatagtg gagggaacat aacacaccac cagttttttc cttcaccttt
     6061 cccaatgata atctttaatg ctttatattc accagctgga agaactatat tagaatattg
     6121 tttagtaggg aaattgctat attgtatacc tacctttaca ctgtaattat atccattttt
     6181 aagaataata tttttactta tttcttccag actactatat tcctttttta taatagcttc
     6241 actttctttt atactttttg aattttgaag ctttggctgt aaatatttta ttacttcatc
     6301 tctaactttt agtttaagtt cttggtcttc atctgtattg ctgtttgcta taacatgaaa
     6361 tcttattaat ttatctttat aatcttttga tatattgtct acttttttta cctcaccatt
     6421 tattactatt gtcattattg atatgactga tataagactt aatattaata gtcccaatct
     6481 aatcttaatt tttctcattg aaaattcccc ctcaagataa atttatatca tcaattatgt
     6541 ccaattaaat ttaatttatt cataatattt tttaatacta ataaaaaaag ctatcttaaa
     6601 atgattacaa tcattttaaa atagcttttt aaaccttata ttttataagt ctttactaat
     6661 catagcctta tctatcataa actgctctgc tttttctata tgctctgtag catacttctt
     6721 ggatagctca ctatcacctt taattatagc atcaagtaag tatttatgct cttcaacaat
     6781 attatgtgat gtatcataag cagacatata ggtaactcta aatctatata cttgttccca
     6841 caatgagttt attaattgga ttaatttttt attattagtt gctttaaata tacattcatg
     6901 aaattctaca tcaagtttta ataacgcatc tacatcggat tccaaagtgc tttttttaaa
     6961 ttcttctgat atatctttaa gctttttaat atcttcatca ctaattcttt cagcggcaag
     7021 atatgctgct aagccttcta ggcttgctct tacttctaat acatccataa tatcctttaa
     7081 tgacatattt gccacatacg caccttttct aggcaacata acgactaaac cttctaattc
     7141 aagctttctt attgcctctc tcactggagt cctacttact cctaattgtt ctgctagttg
     7201 cacttccatt agcctttgac caggctttaa ttttccttct aagattgctt ctcttaaatt
     7261 ctcaaagact acatctctta atggcttgta attatccaag ttcagtttag ttaaattctc
     7321 cacaaatttc aacccctttt tgactgctat taacaacata cacctgttta tattttttta
     7381 ataattcttt ttttccaatt agagcatctt ctttattttt aaatagtcca aaaactgtag
     7441 gaccacttcc actcatcata gaacctaaag cattattttt catcattact tgttttatat
     7501 cacttatttc tttgtgttta cctatagtaa cattttcaag aatatttacc atgctttctg
     7561 atacagcttt tatatcttca gattttaaac actctatcaa atatttatta tttggtcttt
     7621 ttttaatatt attcaaatct aatccttgat aaacttcttt agtagaaacg aataagtctg
     7681 gtttgcatat caatatattt gtatcacatg gtaaaccttt gatttttgtt agcttttctc
     7741 caataccttg agctaaggca ggtcttcctg atatacaaaa aggaacatct gcgcccaaat
     7801 taagtcctat ttcttttaat tcatcttcag ataatctaag ttcccatagg tgatttaaac
     7861 ctactaatac tgcggctgca ttagaacttc ctccagccat ccctgctgct acaggaatat
     7921 ttttttctat gaatatttct acaccttttt ttatataaaa tttattttta agtattttgg
     7981 cagctttata aacaatatta tcttcatcta aaggaatatc taaactagtg ctttttacct
     8041 ttatttcatc ttcgtctaat tcttttatct ttactatatc gtataaatct atcgtttgca
     8101 ttatcatttc aacaaaatga taaccgtctt gcctttttcc caagacatca atggataaat
     8161 ttatttttgc tctactcttt agtcttataa aatccattct atcctccttg tttaaagaat
     8221 aatgcatcac taacaataat attatactac aaaaaataaa ataatagggt attataatac
     8281 cctattatac ttaattactt taaatttttt aatattaatc tactaatacg actttttttt
     8341 ctaaaataag acacttacca ggcttaattg gttcatcaag ttttatatca ttaagttctg
     8401 caatttcatt ttctgttgta ttgtattttt ttgctatatt ccagaaagta tctccttctt
     8461 tgcatatata tacgattatg ctaggagctt tagataaatc atatacacct tggtcttctc
     8521 ccttgataat aaaattttct gctttcttat ctaatgcttc agtaaatctc ttaattttga
     8581 taatcaagtc tatctggtcc ctattcaaat caacttcgac tttatcaata catgctgtat
     8641 taaacacaga tgctgtatca gttagattat ccatttctat atcatgttca aatggaattt
     8701 cctcactaat tttataaacc aattttaaac cttcaactgg gacaaataaa atttcaactt
     8761 ttatgattcc ttgtataatg cttttatttc cttcaatata actattttct atagacatgg
     8821 ttggacaaac gcttacaata tcttttattt gtatatcatc attatcattt cttatgcctt
     8881 ctctaaccat aaatgtttct tcactacgtc ttaaagtctt atttaattga ataggtttgt
     8941 ggtcaaattt tattatcttt tgaggcgaat atgcatcttg taatacttct cttgtaactt
     9001 catctgtaac ttttacctta caacaagcca tacaatctat ttctaaaaga cctgtgttgc
     9061 tttcactatt ttgcttgaaa atatgattaa aatctgacat agacaacaat acttcttcag
     9121 tcataccatc actaactcca gggacttcta caaattgagt aaactcaata cctactctat
     9181 ccaactctac aagttcaccc tcatatgtac aagctaacgg atttatttct aatactccac
     9241 caataataac tttattgtca gtcactctac tttcttttac tttaacacat ggatttaaac
     9301 ttattataga ttgaatttcc tctgtgttta ttgttatagt atctcttata gaactttctg
     9361 ctttttctat tccaactata tcttgaaaac aaatttcctt tctgtgcttt tgtatgcctt
     9421 ccacttgcgc cacatctttt acaatgtcta acctttgctt ttcaaacaaa cttcccctta
     9481 tattcataag agctcctact ttaatctttc tctcattcat tatagtacaa tccatatgct
     9541 ctacttctga aaataacatg tattccatat cttgaacaac attgtctttt tctattactt
     9601 cgtttatatc tacttttccg tctacattag aaatagtatt tttatcatct gcaatataga
     9661 ttacattata attaaaacta cctctgcaaa gtatcttccc atctgccatt tcaatttttt
     9721 taagtgatat gtatccttct gtttttacaa tttcatatac atctaatttt ttgtctggta
     9781 caactgcctc cgcttcaatg aatgtttgaa attttccaaa gtctattcta ttgtcaactt
     9841 taattacatc tttaattaat tccatagtct acctccttaa gtattataaa aatatcacac
     9901 tatatttata tttaaggagc aaaaaaaaaa ttacacctat ttttaaatag gtataatttt
     9961 aacttatttg taatttatct tgatctctaa aaacttgcaa ctttacatta gatgttaata
    10021 aatctgagta actataagat actctaggat atccattact ttcatcatct aactttataa
    10081 caaacacgct tggataaact ttttctagta taccctcttt tgtaataatc tgctttcttc
    10141 ctttattagc tttcaacaat attttctttc ctatatgtct ctctaagctt actcttatct
    10201 tatctagagt ttgaacagta gccacaatat caccttcttt aacgtttata tgtttacata
    10261 atatcacata taaacatttt tgtcaaatgt ttaaactaat tattttatat attttttgca
    10321 aaaatgtcaa tactattttt gaagatatac agtataatta tgttaatttt tgctaatgta
    10381 atattgtttt tggcattgcc catctaaatt ttcctctggt ttcagaacca ccaattccct
    10441 taacacctgt aatagtctgc tctataacct catcaatagg aactactaca tcaattcttg
    10501 aacaagcaat tttctccact ataaaaacag ggtctaatgc atgtatcatt atccttccag
    10561 gcgaacttgc atagtttgct cctgcactta ttattttttc ataatttgat tggcatgctc
    10621 ctgcgaatat gacaagatta tccaaatttg gttgccattt acgtgcttct tttactgttt
    10681 taacaaaatt tgaagagttt ctatagttac tcatatcttc tatatctcct ctttttattg
    10741 tcattgcatc atgacctgtt attactagta tatccggatt atatttttct agtagtgctc
    10801 ttacttcttt atactgattt tgctctgaaa cagctacacc tactgcaggt attccaagtt
    10861 gagcatatac atccaagcaa atttttaaat attctttatc tccatctatc tgtagtactt
    10921 ttccaggcat tccatatgtg ttaggattta attgaagttt tggaactgct ctagtcattc
    10981 ttttttgtct ttctttagct tttcttactg acttatataa taaattttct acatttttat
    11041 ctattaatat atcttttata tcaggtgctt ttactaattc taggtcatca atataagcat
    11101 ctgctattac tctaaatgca attcccttta gtatagctat tttctcatta ttttcatcta
    11161 caccaaaaga gactatttta aatactatat ctttgttatg tgactttcta gctacaatgt
    11221 ctcctacctt catagttaaa cctccaataa atttttatct tagtttatac tatgttcgat
    11281 ataaaacttt gtttcctaat gtcaaaaaaa catttcttat aatacttctt ttatcactct
    11341 taaagcattt ttatagtata tcttttctat atcatcttca ctaaaccctt ctttttttag
    11401 aggttcatac agtttaccca tctgagatat atcttcaatc tcaacttttg aatctattcc
    11461 atcaaaatct gaccctagag atacaacatc tattcctcca acatttacaa tatgttttat
    11521 gtgtctaacc atatcttcaa gtttactttc acttttatca cttaaaaata agtggaagaa
    11581 gtttatacct gtaactccac ctttatttgc taatacttta atcatatcat cagttaaatt
    11641 tctagaatga ttcatcattg ctcttgaatt agaatgtgta gcaattattg gtttagaaga
    11701 aatctttgca atttcataaa atccaccatc tgaaatatga gaaacatcaa ccaacattcc
    11761 caattcattc atcttatgta ccacttctct tccaaaatca gtaagccctt tttctctata
    11821 ttctacttta ttatgaggaa agctcaattc attcacatga ttccaactta ttgtcatcat
    11881 tctaacccct aagtcgtaaa atttctttaa attttctaat ttaccttcaa gtactgcacc
    11941 ttcctcaata gataaaagag ctgtaagttt accttctgat tggtttttca taatttcttc
    12001 atagttagta gctaaagcta tttcatcact attttttttc atttcctcat gaaatttatt
    12061 tgccatactc atgcaataat caaaaggatg ttttacttct tcagtatcaa caaaaagagc
    12121 aaatgtttga gctaatgaat cccccttttt caatctgtct atatcaacag aatatttatt
    12181 agacttaagt tcacttgttt ctacattttc cattaatttt gctatagtat cacaatgtaa
    12241 atcaataaat ttcatattta taccccctgt atattttata tttatatcta aaaatagaaa
    12301 tagttgtcct tatagacaac tattgacaac tattttttaa tattcttttt aatattactt
    12361 aatcttattc ttcttttaag ttaggtatta cttttttaac ttcttcaatt tgatgccatg
    12421 ttttatctat attattttca ctaatatatt tttttaaatc agttaaagat gtaaaatctt
    12481 taactgataa attgtcatca tcatctgcaa atcttaaaat aaacatgaga ctcccttact
    12541 ttccacaaca tttcttatat ttttttccgc ttccacatgg gcatgaatca tttcttccta
    12601 tttttgcttc tttaactatt gttttagact tcttataagc ctttattatt tcttttcttt
    12661 ggtctgctgg tagtatatct tcccaacctt gtaaattata tagccaatga gcctcaacag
    12721 ttaccatatt gttatataat ttttcccaat ctatttttat agaaacttct ttatcagctt
    12781 ctacttcttc taaaactata tcttctttta agctttcact tattccatct atgaatccca
    12841 tgaaaaattc attagtagtt ccaaattttt cagccaattc acttacttta ccttcaacaa
    12901 catccatttt attatttaat atttcattgt atattccagc ttcaactttc aaatattctt
    12961 cccagaattt tacttctgct tcttgacttt catgattatc acttaaattt ctccattcag
    13021 tatataaact catacttttt cctcctaagt atacctatat ttttatagtt tttatagtat
    13081 aaaatctttt agttagattg gaagtttctt ctttttgatt ataccatatt ctatttaaag
    13141 ttaactctta tattatatca taattaaaca attcttttaa tacttttata gcatactctt
    13201 ggtttataaa ataagggctt gtcgcaattg ttattacttt ggctttttta attaagtttt
    13261 ttatcttgta aaatttgagt tcaaatggta tatagtccat atcacttgaa aatatatcca
    13321 aatctatgtc aagaacaaac tctgattcta cttctaaatc aaatccatat agactatcta
    13381 ttattattaa ttctgaaaat atgttatatt ttaaagcagg ttttataaaa cttcccacat
    13441 ttaaaacttc atttgtatat ctaaatacat cttttagatt atttacatct acatcatagt
    13501 tctcaggttc tctagtatcc ttatgttggt ctacatgcac taacttgcat ccttttgtaa
    13561 actcaccctt taacaaagac tttgcccaaa aataaaatgc atggttatga ttgtcaaaca
    13621 catatatctt tttatttccc aggacatatt cgaccatatt ctccaatcct tttgctttta
    13681 tttccttatt ttcatctact tcattaaata ctacctcatt gcctaacctt acatcattta
    13741 atgtaccatt tataagtttt ggaacgtata tgctcttatt ttctctttta tcatatgaaa
    13801 atacattatt tcctactggt ttttctatat aaaatccact atattcataa ttttccataa
    13861 aactttattt cctccattgt tatataaata tataagttat aattttaaaa attaaaatgc
    13921 acttattgta aatcatatca tctattatag aaaacatctt atataactaa atataatatc
    13981 ttctctcaat atgtattata caataagtgt aaaatatatt tatataaaat atactacatt
    14041 cctataacaa aaccatcagc ttcaattgga agataactaa ctcttctatt caactccacc
    14101 tttccaaaaa atactaccca atatggtcta aaaatacttt caattttaac tatttcaatc
    14161 tctgggattc ctcccataaa cctatgagtt aatctcatag ccattttatt tgcctttgct
    14221 tttactcttc gttcgtcctt atcactatac tgcacaatat tacaatccac attttttaca
    14281 acttcatatg taggaaaatc ttgtactata ctaccagaac ctgtagaacc attcactaaa
    14341 atttttaatt catttatata attagaatat ctcctattct tgaaaatgga tttcgttttg
    14401 tgaattttaa gtgtgataat tttaaactct atataatgaa gtctaacttc atgagcatat
    14461 ttgttcttta atattagctt actaaataaa gaattttttc cacttttagc tatctctata
    14521 gcttcttctt ttgtaattct tataggtaac gctcttatat ccataaaatc attctctttc
    14581 ttattttttt cttgtcattt ttaaaactct atatatataa tcaaaaaata ttacaattac
    14641 aactgacata actccatatg gaattatttt tccaactata atatcatttc tagtcaatat
    14701 accataaaat ataaatcctg ttattaaaaa gtacaataat actagaaaac cgtctattcc
    14761 aagccatttt atttctaaat ctttttttac cttagacact aatttagaca tagcagtttc
    14821 tccttttata gattattttg tgtatttaaa ttttcaccta ttctattacc aatcatccat
    14881 gcaataaatg aaaaaggtat agctatcaca agaggctcta actcaaagct ctgaactgga
    14941 aatattttaa aataaccaaa agtcactcca ccaacaatca ttcctaaaat accacccata
    15001 gatgttcttc ttccatttcc aagaagccca cctactatag gtacaaatgc cgaacccatc
    15061 caaatacttc ctacaaatat acatgcatca tatacatttt caaatctaaa tgctacgcta
    15121 agccccataa ctgctgctaa acatacacct attctagtaa actttactaa ttgtttctga
    15181 gataactctt tttttgtaat tcttccatat atatcatttg ctaatgtagc cccagctgct
    15241 agataatagc tatccaatgt tgatattata gaacctaaaa ttcctattat aaatattact
    15301 cttattcctt gtggtaatgt atctaaaact aaagttatat atccagcagc tggagccatt
    15361 tctggataag tagctcttac tataagtcct gtcatagtta acaccatgtc aaaacatatc
    15421 catagtgaaa aacaggttaa taaggctctt cttccaacct taggagaatc tgctgctgaa
    15481 aatctttgat aaaaagttgg gtctgaataa gctccaaaag aatatacaat taacatcaat
    15541 gctttcatag ctgacatacc ctttgtaggg tgcataagct gtggtgcatc tttgagtgct
    15601 tctgtaagac cttgaaaccc tcctatttgt tcaaattgac ctggcataac catagttaaa
    15661 ccaaatacca tacaaaaaaa caataccata tctgtaatgg caactcccat aagcccacca
    15721 agtactgtaa gtacaacgac tactcctacc attattaatc ctattaattc tttatctatt
    15781 ttccatgcta cgttacctat agttcccata gcagttattt ctccaagttg tgcacaatat
    15841 ataaacatca aaattgcccc aattatagca acatgcttgc cataaaaact ttccagtaaa
    15901 tcaggaactg ttatattggt tcttatatga atccttggac caacccacaa tgctaaaggc
    15961 attctcccaa tatgtgcagc tattgaccaa actgcccatg cagatattcc atatatagcc
    16021 gcatactcta cactacctac tgtaccaact cctccatacc atgatgcaac caaagttcct
    16081 actattaagg accatggtaa acttcgattt cctaggtaat aactttccat atcttttaca
    16141 tttctagaaa aataataacc tattcctaat aatataaatg caaatataac tataatccca
    16201 ttttcccaaa atgttaacat atctaatctc cacattctta tattttaatt tttaacttta
    16261 atatctaaat atgaacaact ctacccattt ccataaaaat aggtagagtt atataataat
    16321 tataagtatt taagtaatct tgttattaag caattttatt gtttaaacct ttattttctc
    16381 ttttatttat tttatttaaa gcataagtag caaatatccc tataactgca catactacag
    16441 ataaaataaa tatatatgta tatcctgttt gtccatattt atctaaccaa ttacctgcaa
    16501 tagtgtaata aaacgcatct ggagaaaatc ctataaatga agcaaatcca actactgctc
    16561 ctgtagtttc taaagatata ttagactctg aaacagatgc aaagtatata cctctaaatc
    16621 caaataaaat tacacttagt attatcatgt ttattacagc tatccatata aatcctgtac
    16681 tcttaggaat aaccaagtaa gcagctgtac ttactgtcat taatataaat cctacaaaca
    16741 ttactcttat agatgaacca actttatctg ttataacacc agcaactgga gatgctccca
    16801 tttttataac ataagttctt attattgata aagcagaaac tagagtcatt ggcattacat
    16861 aaacttctga taagtatgga cttaaataag ttaaacttga gaatactaaa taagttgaaa
    16921 atattataag accaattagc caagcctttg gcattgtaac tacttttaac aatgacttaa
    16981 tacttaatcc ttcatctgat gctccactat cttttttctc ttccactaag aaaaatagaa
    17041 gtataccaca tattatagat gctgcagaat ataaccatac tacatatttg aatcctactg
    17101 ttatttcagc aaatttacca aagaagtaaa gacctataaa agaaattaat gtacctgata
    17161 cacctgataa cccttcataa aaaccaaata ttcttccttg ctctgactca cttccttgca
    17221 ttctaacaac cttaacagag gctgagaaga aagttaatat tgttgtaaat ccccataata
    17281 caaatattaa aagtatcata ctatatgatg ggaatgtaga gaaataaaat ccaagtattc
    17341 ctgaagcaat taaagaaaaa gttattaatt ttctaactga gaatctatct gcaaaccatc
    17401 ctcctataaa ataagatacc attcctacta atccatataa actcaataag ttccctattt
    17461 gtgtatgact taatgctaaa gcttgttgca ttgggtcata aaatgatgat ttcatatatg
    17521 gtaaactata catagctgtt gttccaaatg ccaatatgaa tattatcaaa aatcttttaa
    17581 cattattttt gctttccatt gattctctct ccttaaaatt ttattgttta taatctcatt
    17641 catataattt tattttaatt tttctattgc ttgtttaaaa tcatttatta tatcatttga
    17701 agattctaat cctacagcta ttcttattag attttcccca ttcatttgaa ctaatgtatg
    17761 ctcttcccct aagcttgttg ctattgttgc aagctctaaa gaattcatta atcttctact
    17821 agcttggtct cttgtcaatc cattgatacc atccttaagt ttaaatgaaa ctacacctcc
    17881 acctaaacca ttcatttgag ttttacaaag ctcatattga ggatgagatt ttaatgctgg
    17941 atactttacc aattccacat tctcttcttc ctctaaaaat tctgctactg ctaatccatt
    18001 ttcacaatgc tttttcatcc tcatatctaa cgttttcata ccccttaaaa tcatccatgc
    18061 actcattggt ggtaaagatg ctcctgtaaa acaacataaa cttggccatc taactaaatc
    18121 tattaattct tttgaacctg ctactgctcc ccctaaagta tccccatgtc catttataaa
    18181 cttagtaaga ctatgtatta ctaaatctgc acctaaatct attggtcttt gtacaattgg
    18241 tgtcccaaaa gtactatcaa ctatagctaa gcttttattt tcatgagcaa tttctgctat
    18301 tgtttttata tctactaagt ctaatgatgg atttaaagga ctttctatat aaactatttt
    18361 agtatttggt ctcattgctt cttttacatt ttgtggattt actgcatcta caaatgtaac
    18421 ttcaactcca aatttagata acaactcaga catgaataat ttagtgtgtg aaaatattcc
    18481 atctgaacta actatatggt caccactttt taccaatgct aatatagtag atgttattgc
    18541 ccccatccct gatgtactag atatggcact ttctgcattt tcaagcatag ccaacttagc
    18601 ttctaactct gtaagagtag gattacactc tcttgtatac acatatcccc atgtcataca
    18661 caactcttca aaatgctcta ctgtctttgc agcaaatgtt gctgtttgac atattggtgt
    18721 agccaatgca cctgttgttg ggtctgcact atgtccccaa tgtattattt ttgtctctaa
    18781 atcttgatta atattgttac tcattttgat ttcctcccat tttgacttat ttatacaacg
    18841 ttttccctac caaatttcat caatttagct tagtcttaat acattttttc aagtatactt
    18901 ttttatttgt tataatttct aaaaatatga cacttatttt gtttttttac agttctaatt
    18961 ataaactcta tagtaactat agagtcaata tgtttttgat atttttttta caatgtttaa
    19021 aatagtcagt acaaaaatag gtatatctta gaataactaa agtcatccta agatatacct
    19081 atttttatat cacaatataa ataattacat ataagcatta gtttttttat cataaataga
    19141 tttttcaaga tttatcatct tttcataaat atattatagt taaatatttt aatattacct
    19201 aacatctaca ataaaatata ctcttaaaat aaaattatat aattaaaaat actctataaa
    19261 agctaaaaag tttttctatt cgctacatct aaaaataatt agatagaggt ataaatgtat
    19321 ttagtttatt tatacctcta gtcttttata aattatatct ctactaaatt gattttttag
    19381 cacgaataag tgtgctcttg gagataccag tcatcttcac aacctctcta tatggcttag
    19441 aatttaataa ccccaatgca tattgtatct gttgattgct gtatttcttt ggcctaccat
    19501 ctttaaagtc ctttttcttt ttagcaaatt cttttcctaa ttgagtacgt tctattatca
    19561 tatttctctc aagttctgca actgcaagta atgtagtcaa aaagaattta cccattgaag
    19621 tattttctaa aaggcctata tttaaaatat gaacacttac ttctttagca aataaacctt
    19681 ctattagttg tattccttct gatgtacttc tagctagtct atccagctta gttgcaacaa
    19741 gtaagtctcc cttttttaga gatgatataa ttttttcaaa tttaggcctg tttgtagtag
    19801 tgcctgtata agtttccttt attattttag catcattata tttattcaaa atttcttgct
    19861 gttgctgata caaactgttg ttgtctgttt gattttttgt acttacacgg caatatccat
    19921 atataataat catcacttcc taacataaag attttgtaca gaagttaccc agaagcttgt
    19981 ttttacttat ctttatccat tttactaaaa cgatttttgg gtgtcaatac tgttaacttt
    20041 tgacaccata ataattcctt tataatttgt ttgactatgt caaaaattac caatataatg
    20101 aaattaatga gtttatacaa attggaggaa ataatatgaa tagtctgtta aaagaagcaa
    20161 tagatgaatt aaaaaacatc cattgcgggg aagttttttt attaaaagaa ttgttcaaag
    20221 gctatgaatg gaacaggttg gagatgaata tcagattgaa attaggaaaa ctttttttgt
    20281 atgaagtggt aactaatccc aatttaaatg tagaggtcat caataaaaat tctgcttatc
    20341 agcaaatgta taaaaaaaaa tagtgactta aatgtatata atagtataat cttcttattg
    20401 attcaaaagt taaaactttt aatacaaaac aatcattcta ttttaacatt tatcaatatg
    20461 tctaaattta tataattaac acatacgaat agtattgttt aaatcacatt atttaagata
    20521 gataaatctt aaccctactc cagcataaat actaaaatag ggcttagaat tatctatatt
    20581 tttatcccat aaaatgctta gctcttcctc ttgattgaac atatatatct ggggaaatac
    20641 aatagaagcg taaatttata aaatttccaa agtcagaaaa cttcagttca aacttaggta
    20701 taccaaaact ttcaggtgct atttcgtcaa cgccaaaata gatgataatt ccattatctg
    20761 taagataaaa tgcttggtct tcaggtataa ctaactcaac attagaataa tacatttctc
    20821 tattttcatt tatttgagat ttaatgtaat cagtaattat attaatataa tcaactccat
    20881 ctctaaaaat gtcattaagt aaaagttctt ttccagttgt caaatctata ttgtaattat
    20941 tcaatgtatt gtatttaggc ccatagttat tagcaaaccc cataaggttt aatatcatac
    21001 tcagtacata atttttatta aaagtaactg cgtaactggt agatacccaa tagctttgtt
    21061 ttggaagtga attttgagaa gttgcgttat tatattctcg ttcttcttcc tgtaagccat
    21121 ttttaaaatt atttacagtc attcttacag cattgtttat gttatttaaa ataactgggt
    21181 ttacatacgt ggattgattt ctaatattgt atataacagg atactttaca tcatatgaaa
    21241 aatacttact tctttcaaaa tcttcattaa attgtacata taatatatta tatgtacagg
    21301 tatttgtaga ttgataattt tccattaaaa tcctccccct tttataaaaa aatcagatac
    21361 aatataatat gcttaaaaca gtaataatgt taatggtaaa tgtaaagaat agaaagtatc
    21421 taaaacagca aaaaatgact aattttgtgg tataatacat aggattgaga gttattttat
    21481 aggaggaaaa atatgttatt attagcagat aaatggaaag attatgaact tatagatatg
    21541 ggaaatggag aaaaattaga acgttgggga agttatgtac ttagaagacc agacccacag
    21601 gttgtatggc caatggaatc tgaatgggct ttatggaaaa accctcatgg acattatcac
    21661 agaagcaata aaggtggagg tcaatgggaa cacaaaaaaa gatatccaga acaatggact
    21721 ataaattata aaaacttaaa gtttcacatt aaaccaactg gctttaaaca tactggatta
    21781 ttcccagagc aagcagctaa ctgggattgg atgatagaga aaataaagaa agctaacaga
    21841 cctataaaag tgttgaatct ttttgcatat acaggtggag ctactgtagc atgtgcatct
    21901 gctggtgcag aagtatgtca tgttgatgct gcaaaaggta tggttaattg ggcaaaagaa
    21961 aatatagaat tatcaggtct taaaaatcaa acagtaagat ttatagtgga tgatgttgta
    22021 aagtttgttg aaagagaaat aagaagaggt aaaaaatatg atgcaataat aatggaccct
    22081 ccttcttatg gaagaggtcc taaaggagaa gtatggcaaa tagaagaaaa gttatacagt
    22141 tttgtagatt tatgtatggg agttttatca gataaccctt tatttttctt aattaattct
    22201 tatacaacag gattttctcc aatagttctt gaaaatgtat tgtctacaac aataggtaaa
    22261 aagacaaata caggagatat atacggtggg gaattaggaa taccagcttc cagagatggt
    22321 aagatacttc cttgtggtat atttggaaga tgggagaaaa aataatgata aaagtcatat
    22381 atgaagataa ccatttatta gttgtagaaa aaccagttaa catattgtct caaggagata
    22441 atactaatga taaagatatg gtaaatcttt taaaagacta tattaaaaaa aagtataaca
    22501 aacctggaaa tgtatatatt ggtttaatac atagattgga tagaccagta ggtggagtta
    22561 tggtgtttgc aaaaacatca aaagcagcat ctagattgtc tgagcaggta agaagtaaga
    22621 ctttcaaaaa aacttatcta gctgttgttc atggcaagat aaaatctaac tcagatatac
    22681 taaaagattt tttatataaa aataaaaaaa ctaatatggt aactgtagtg aaaaaaagcc
    22741 ataaagatgc caaagaagct gaacttaact ataatgtatt agattttaaa gaaaatctaa
    22801 gtttagttca agttaattta aagactggtc gttctcatca aataagagtt caatttgcaa
    22861 gcagaaaaca tccattatac ggagaccaaa gatatggcaa agaaataaat aagattggac
    22921 aacaaatatc tctttggtcc aataagatag aaatttgcca tcctactaca aaagaaaaaa
    22981 tggaatttac atgtcatcca cctgacaaat atccatggaa tatattttaa atctcaaatg
    23041 aggtgattat atgagtaaaa aattttcaat aggagaaatg tctaaacttc ataaaataag
    23101 catacagaca ctaaggtact atgaccaaat agatttattt aaacctgttc atgttgacca
    23161 agaaagcaat tacagatatt actcaattga ccaattttca caattagata ttataaaata
    23221 tttaaaatat ttaggtatgc ctttaaaaga aattaagtca aagcttgatg aaaataatga
    23281 agaaattcta aaattactaa caaataaaat taatatagtg gatgaaaaaa ttaaagaatt
    23341 agagcttata aagaaggtac taagcacaaa gaaagaaact atggaaaaag gtatgaaagt
    23401 agatgaagtt ggtaaaatag tgagaaaaca tattccacga agaagtatct tatctataga
    23461 gtatgaagaa ggattaagta gtgaagaaac tatggaactt tccagaagac aaatagcaaa
    23521 tatattagaa gataatatat ctgtgttcta tggtggagta agtggacttg tatctataga
    23581 taaattttta aaagaagata aagttattta tgacaatagt tttgttactg ttgaaagaga
    23641 cttatttgat ataaaatcac aaaaacatat aacagaaatt tcatctggtg attttatttg
    23701 tatgacatat aaagggcctt acaaagaaaa ttataccgct tttaagaaac tggttgatta
    23761 tgttgaatat aaaagaatac ctattgaaaa ttatatatat gaaataccta taatagaccc
    23821 tctgtctaca aacaacgaag aggaacttct aacagaaatt caaatacaag ttaaaaagag
    23881 agaaattgac ttaagatgtc tagtgtagta atctagttta cttacacatc ttaatatagt
    23941 tgtctattat caacatatga tttttctaga tagtgattcc taacagtgag attttcaaaa
    24001 aataggagga aatatggcta atgctaattc cctcctattt ttaattaagc catatcattt
    24061 taattaagcc atgtcatcac gaagtgcatc aataatattt tcttttttta tcttattaat
    24121 agtatacaac attgtaataa atactataaa taaaacacta aatatactaa ttccaatact
    24181 aaaccatgga atcacaaaat caatatcatc tgtaaccatt actttatgaa ttagccatga
    24241 agaaattact gctataggaa gtccaaagaa caatgctctc atgccataaa aggcacactc
    24301 aaaattcatc atcttttgga aatcatgctc agacattcct acagagcgaa gcatggcaag
    24361 ttctctacga cgtaacttaa tattagttga aattgtatta aacacattgg caatcgaaat
    24421 cagagaaatc ataattatga aagtataagc aaaaacattg gcaataaaaa tgtaattacg
    24481 gttctcatcc atcattccat acatattgta cagattgtac tcattcgtaa ctccctcact
    24541 ctcaataatt tgctccattt ctgcaactga ctgagtagga tttttcgatt gaaagcttaa
    24601 actttcaatt cctgtagaaa caccaggaat ctcaaactta tctttggctg aataaggagc
    24661 caatactctg aagcttgctt tcttcacatc tgaacttctg attcctggag gagaatcagg
    24721 tggcactact ttgacaaatg aaatattcac attttgtctc tcaggtatct ccaacttgcc
    24781 attagtttca ggagtgaggc taacattcat agaagaactg ttgaaaatat caataatatt
    24841 attatccttt gttaccatag aaacagcaat cagttttgca ttttgtccag tatattcttc
    24901 tgctggtaat ccaagacttt taataatatt cagataagtt ctatcatcaa gaaattgcat
    24961 atctactggt aattcaagca tttcactcat agaatgtgaa ccctcatagt tcaaataatc
    25021 gtctgtaagc tcacttactt tggcaacaca tgaataggtc ataagtttct gatacaaacc
    25081 ttcgtaaaca ccatttgcag tttttagctt atcgtaaagc ttcaacattt cgttgtcttt
    25141 cataccttgt gtagaaaaaa taatatcctg gtcagtaacc tctgacctct gatttgacat
    25201 ctgttttagg tctgctacta aagcacttgt agatataaat agaactatgc ttaaaacaag
    25261 cgacaataca atacttcgat aacgtttctt gtttctttta aagtttttca tagcaagaac
    25321 tccctcaaga ccataaactc gctgtgttag ttttgatgtc ttcacagatt tagattcaat
    25381 ttttacctca tttgtctgac gaatacactc catcactggt gtgttggcag cctttcttgc
    25441 tggtatgtaa gcagaaatta agattgtaac catacttacc actgctgcaa cgatgattgc
    25501 agggatagac aatgttaatg tcaacggaac gtttacatag agaatatttg caaagttttt
    25561 agctatgaca gaaatcacaa gtccaatact gactataccg attgcaacac caattggtat
    25621 accaatcgca ccaatgcaaa ttccttcaaa tagcaccgaa tttcgtaact gttttgctgt
    25681 agctccaaca gatgaaagta ttccaaattg atgtgttcgc tcgttcaatg acatgttaaa
    25741 tgaattatat attagaaaaa ttgagcctag catgattatg gcaagcacaa tgccaccaac
    25801 cgagtacaaa aacatattga acaaattgtt atcagaaaca cctatgaagc gcaacacatt
    25861 gtcattgaaa atggaagcat ggtctttagt gctacttatg taagagcgaa tctgatgtgg
    25921 atttttcaat gtgacaaaaa ggctaagact gtcagtcttg acttgtttat cttctttggt
    25981 tatcaaggta tatcctggtg cagaagattc ttcaaatacg ggctttctac agatacccac
    26041 aactgtataa gttttcttat cctgtggcac aaaaacttca ctcccagcag tgtatgggtc
    26101 actctgacca agcttttcat tttcactcat tcgtttccct acatccaaag aaatagtatc
    26161 acctactgca aatttgacac caccatctgt cgcaacttta cttgaaacaa gaatctctct
    26221 actattttca ggtaatcttc cagaaattaa agttataggc aaagtatcaa aagcttcttt
    26281 gctaaatcca gcaataaaaa gatatggttt atctggattt ctgttacctt caagtgttgc
    26341 atagccaata ttttcaaatg ttgcagtatt tgcaacttca tcattgactc tttgttcctg
    26401 tacaaaagaa gaatccacat ccaaaaactg gacatgccaa tcaccatatt ttataatagc
    26461 tccatttgtc atatagttca tcaaagaaac tccaaaggta gtaactgctg taatcatagc
    26521 agctgacaga acaactccta taattgttac aagagttcga gtgcgattct ttttcaatcc
    26581 ttgcagagca actttgttaa aaatgttcat taacgattca ccttcctctc atcacgcact
    26641 acttttccat ctgaaataca gataattcta tctgcctgta aagcaatgtt ttcatcatgt
    26701 gtgacaataa tgagagtttg gtgatattta ctatggctct ctttcagtaa ctggataatc
    26761 tcttgtccat ttttactgtc taagctacct gtaggctcat cagcaagcat aactgctggt
    26821 gcattcatca atgcacgacc aatggcaaca cgttgctgtt ggccaccaga aagttgattt
    26881 ggtaaatgtg tttttcgttc ttttaaacca agcaattcta acaagtcatt taaccgttct
    26941 tgattgacct tacgcttatc cataagtata ggcaatgtga tattttctac tacatttaat
    27001 gtgggtatca gattgtgaaa ctgataaatc aatccaactt gtcgcctacg aaaaatagca
    27061 agtttttcat tgctttggcc atatacatct tgcccctcca gatatatctt tccacttgtt
    27121 ggtacgtcta ctcctgcaat ggaatgtagt agtgtagatt taccagaacc agaagaacca
    27181 atgattgcag taaactctcc tttttcaata gtaagtgaga catggtcaag tgcagtaacc
    27241 ttattttcat tcttaccata aaccttacat aaatcttcaa tctttaaaaa cgccattata
    27301 taagtctcct ttcatatttt ataaatatat cataggactt tcaacttaca tttaagtgac
    27361 atttaaatga gagattcgtc actttggaaa acgaataaca aatattgcac caccttgtgg
    27421 gtgattttgt gcagtaattg ttcctccttg tctcataata atcgtcttac aaagtgccaa
    27481 tcctattcca taaccggctg tgcttgaatt tttcccacga taaaatctgt taaatagaca
    27541 tggtaaatct tcttttttaa agcctgctcc gttgtcatga atagtaagtt gagtaaacaa
    27601 aaagttgtcc tcacaaatga tatcaatttt tccattatca cccacactct ccatgcagtt
    27661 tttaaatata ttttgaattg cttctgaaag ccaatttaaa tcacccaaaa tccttactcc
    27721 ttttggtata tcaatatgta aagtgatatt gtgcagttcc atgggaatca aaagttgatg
    27781 aagtgcagaa ctaatcaaat tattcacatc aatttgttct ttttgaaaga ctacaatacc
    27841 tgcatctaaa cgtgataatt ttaaaaggga agtaatcagc cagtccattc gtataagtaa
    27901 ttcctctgtt tctcgtatca actcttttct ctcatcttca tcagaagtat tctctaacag
    27961 tgacaaaata agagttactg atgtaagagg agtgcgaagc tggtgggcta tgtcagctag
    28021 tgagtcagca aggtgttctt tttctttttt caatgcgtaa ttttgctctc taatacgtaa
    28081 cgtcatttta gttatttcac tttgcagaat agaaagttca ccctcatctg attcagcaat
    28141 atacagatgg ttagcattat gaagcacaag attaatttgt tctgaaatct gtgcaatact
    28201 tttgtatcga tttttagtaa acacaaaaaa tgctattcca aaggtagtgg cagaaacaat
    28261 agaaagtatt ccagtcataa tactgattgc aaatcccagg gctacagtga gaatagtaat
    28321 taataagaat aaaatagcaa attttcgaaa ctctctattt cgaaacataa cttcccccca
    28381 atctataccc tgtcccacga acagtcaaaa ttatttgtgg acttgaaggg tcatcttcta
    28441 tcttctctcg caagcgtttg atgtacacag tcaatgtgtt gtcattaaca aactcacctg
    28501 ccgcatccca caattcatca agtaatctta ccctcgtaat aatactttta ggattgctaa
    28561 taaacaccaa taacaaacgg tattctaaag ctgaaagaaa aacctcattg ccattctttt
    28621 tcacaacacc ggttgccgta tctacataaa gcccacaaat ttcaaaagtt gacccaaaac
    28681 gcccactttt tcgtagtgct gttctaattc gcgcaatcag ttcacgtgga cgaaaaggtt
    28741 tagtaatata atcatctgca cccatattca gtccagtaac aacacttgat tcatcaccgg
    28801 aagctgtcaa aaagataaca ggaatgtctt gcttttcttt gatttctgtg caaatcgtaa
    28861 acccatttcc atcaggtaaa gaaatatcaa taagtgccaa atcaaattta ttcttagcaa
    28921 tctctacaag tgcttcactt cttgtggata catgggttac tataaatcct tctgaattaa
    28981 gcaaaagaat aagatttttg gcaattgctt tatcatcctc aactaaaaat atccgtttca
    29041 tttactttca tcctccttta ttttattagt tttatatatt ccagcagagc aatagtacac
    29101 tactatttta cttatcatta cacaattttc agtagtttct tttgttctta tataatccat
    29161 gcataatacc tccattgatt tattatattt cttataacat aaatacacaa gataagacaa
    29221 aaaacataat gtaagtgcaa gttttttaac tcactaactt ataaacatac actcctacta
    29281 acactacttt cttgatgaca catcttacaa gcttatattc atacatcaat gaaaaattaa
    29341 atttatccat ttattaaaat aaaaggcaat agcttctata gctaatgccc tttatcttaa
    29401 aatgatttaa atactataca atttttataa atggctataa ataaactcgt atagaatata
    29461 aaatttatct acagtctttt gttatacaaa atttataacc tcttatagaa caaatttacc
    29521 actttgcaaa cttatcactt ttactcagct atattataaa cttttaattt atctttgttt
    29581 aaatgtttct tcttaacttc tactaaagct tttagcgtat ccaaagatgt cattatttca
    29641 gtagaaaatt caatggctgt tcttctcatc ttaaatccat ctcttgtaga gtcatttcct
    29701 ttagttggtg tatttattac tatatcaact tgtttatttc ttatagcatc taatatgttt
    29761 ggtctaggct cttcaattct atttactata tcagcctcta ttccattttc tcttaaactc
    29821 ttcgctgttc cttctgtagc aacaaaagta tatcctaatt ctttcatatc ttttgctatc
    29881 tctataaact catctttatc atgattattt acagtagcaa gtactactcc tctttcatca
    29941 gacatatgtc tacctgctgc caagaaccct ttatataagg cttcttctaa attttctcct
    30001 acacctagaa cttctcctgt agacttcatt tcaggtccta gacttacttc aactttagat
    30061 aatttagaca ttgagaatac tggtactttt actgatacta attttggctc tttgtaaact
    30121 cctgtactat atcctaaatc ctttaattta gctcctaaca tacacttagt agccaagtca
    30181 actattggta ctccacttac tttacttata tatggaactg ttctactagc tctagggtta
    30241 acctcaatta tatataattc attttgaaat tctataaatt gtatattaac cataccaagc
    30301 acattaaggt ctaaagctat ttttttagtg tattctacta ttttttcttt tatttcatca
    30361 gatatatttt gacttggata cattgttgta ctatctccag agtgaactcc agctctttct
    30421 aaatgctcca ttataccagg tatcaatata tcttctttat cacatatagc atctacttct
    30481 atttctctac cagttagata tttatctata agtactggat ttttatggtc tctatcaaat
    30541 gcatcatcca aatactgaat tagtttttct tcgttataag ttatttccat accttgacca
    30601 ccaagtacat aagatggtct aactaatact ggataaccta actccttagc aatttcaaca
    30661 ccttcattag ttgtccatac accttttcct tttggtctat ttatatctag tctctctagt
    30721 aaatcatcaa atttttctct atcttctgct gcatctatat ctctaaagtc tgttcccagt
    30781 ataggtatat ttttttcatg taaaaactta gccaatttta tagctgtttg tccaccaaac
    30841 tgtagtatta caccttctgg tttttctttt tctattatat ttaaaacttc ttcttctgtt
    30901 aatggttcaa aatacaactt atcagatgta tcaaaatccg tacttacagt ttcagggtta
    30961 ttgtttacaa ttatagtttc tattcccatt tttcttaaag attttacaca gtgaactgaa
    31021 caatagtcaa attctattcc ttgacctatt cttattggac cagaacccaa tactacaact
    31081 tttctcttat cactaactac tacctcatca tattgttcat aagttgaata gtaataaggt
    31141 gatagagctt caaattctcc tccacatgta tcaaccattt tataaactgg ttgtatatta
    31201 tataaacttc tcaattcata taatttttct gggcttatct tcattaagtc agatatacct
    31261 ttatcagaaa atcccttttt ctttaattca tgtaaatatt ctttatctaa gtcttctatt
    31321 tttagaactt ttaacttttc ttcttgttct actatccatt taaatttatt tataaaccac
    31381 ttatcaacac cagttatttc tgatatcatt tctactttat agcctcttct tatcatttca
    31441 gctaagtcaa atagtctctc atcatcaggt actactactc tagcctttaa ttcttctaat
    31501 gttctacctt ctgatggagc atgaaccaat gaatattttc ctgtttcaag tgatcttata
    31561 cctttaagaa tagcagcctc aaagttactt cctatactca tgatttcacc agttgccatc
    31621 atcttagttc ctaattttct attggcatgt ctaaatttat caaatggcca ctttggtatt
    31681 tttactacca cataatctaa tgttggctca aaacaagcat aagttttttt agttactgca
    31741 ttttctatct catccaaagt ataacctaaa gctatcttag ctgctacttt tgctattggg
    31801 tatcctgtag cttttgatgc tagggctgat gacctactaa ctcttggatt tatttctatt
    31861 atcgcatatt ctaaactatg tgggtttaaa gctatttgaa cgttacatcc accttgaacc
    31921 tctattgcat ttattatgtc tattgatgcc tttctaagta gttgatactc ttcatcactt
    31981 agagtttgac ttggagcaac aactatactg tctcccgtat gaactccaac tgggtctaca
    32041 ttttccatat tacatacagt tatacagttt ccatttccat ccctcattac ttcgtattct
    32101 atttctttcc aaccttttat actcttttca agaagaactt gtccaacagg acttagctgt
    32161 agtccatgtg aaagtatttc tcttaattct tcctcagtat cagctatacc tccaccagtt
    32221 ccacctagcg tatatgctgg tcttacaact actggatatc ctatcttatt ggcaaactca
    32281 agacctgctt ctaaatttgt aactatatca ctaactatta ctggttgatt tatttctttc
    32341 attacttctc taaataaatc tctatcttcg ccctttttta tagattctat agatgttcct
    32401 ataactttta cattatattt atctaatata ccagcatcat gtaattctac agctaagttt
    32461 aatcctgtct gaccacccat acctgcaagt aaactatctg gtttttcttt ttctattatt
    32521 ttttctataa attctattgt taatggttct atatatattt tatctgcaat ttctttatca
    32581 gtcattatag tagctgggtt actatttact aatacaactt caattccctc ttcttttaat
    32641 gcttggcagg cctgtgttcc tgaatagtca aactctgcag cttgacctat tattattggc
    32701 cctgacccta atactaaagt tttctttata ctatctaatt taggcataac tctttctcct
    32761 tatctaaaca attttcacag ttcttatctt catcatctat acaatcaact ttaattactt
    32821 tattttatct taaattcaaa tttactcatc aatacatcta tttaatacat ctatgttaat
    32881 taaaccaaac tttttgattt aagttattct tgttttttag ttagctattc taatgttatt
    32941 tattttacta attctaaaaa tttatcaaaa atatagtcat tatcttttgg tcctgggcaa
    33001 gcttcaggat ggtattgaac actgtataca tctagtttct catgtctcat tccttcaaca
    33061 gtattatcat ttaaatttat atgtgtcact ttcatttgtt caggaacttc tgaaacataa
    33121 taaccatgat tttgagaagt tataaatact tttccttctt ctaaatcttt aactggatga
    33181 tttccacctc tatgcccaaa tttaagttta gcagtttttc ctccaagagc taaagctaaa
    33241 agttgatgtc ctaagcatat tccaactata ggttttttac caataagagc ttttatattt
    33301 tctataacat cttctaggtc ttctgggtct ccaggtccat ttgacaagaa tattaagtca
    33361 gggtttatac tcaatacatc ttctggagat gttgtagctg gaaatattgt tatatcacat
    33421 ccacgagcta taaatgatct taatatattt ctttttactc caaaatccat aacagccact
    33481 tttggaccat ttccctttat atgctctact tcttttcttg taacagttct aactgcttct
    33541 gtatttgaaa acttatccaa tttatgttta acatcttcta atttactgtc ttctaatgta
    33601 attatacctt tcatggttcc attatttcta agtatcttag tcaaagctct tgtatctatt
    33661 ccttcaagtc cgataacttt attttgcttt aaatattggt ctatatcaat ttcacatcta
    33721 aaattacttg gagaatcact tttctccctt actatgaatc cctttacttg aactttttca
    33781 gactctgctg atgataaatt tataccataa tttcctacaa gaggataagt catagtaact
    33841 atttgaccat agtaagaagg gtctgttaaa acttcaccat atcctatcat tgaagtatta
    33901 aatactactt ctcctacact ttcttctaaa tatccaaatg cctttcctat aaaaactgtt
    33961 ccatcttcta atattaacct tgctttcata ctacagtacc tcctataatt ctctttagac
    34021 aaactaaaga acattaacta aatataagct tttaaatatc tatatctatt tttcactttt
    34081 acattctagt tttatactta tttacattta ttttttatct ttttctatta tactacaaat
    34141 ttcttaaaaa aaagaagtta tctcaaaagt aaaaactcct agaaaaaata acctcttttt
    34201 tttatttaat aattaaattc ttaaactata caatcaactt aatttctatt cagctatatt
    34261 gtaaactttt aactcatctt tatttaaatg tttcttctta acttctacta aagcttttag
    34321 tgtatccaaa gatgtcatta tttcagtaga aaattcaata gctgttcttc ttatcttaaa
    34381 tccatctctt gtagagtcat ttcctttagt tggtgtattt attactatat caacttgttt
    34441 atttcttata gcatctaata tgtttggtct agattcttct actctattta ctatatcagc
    34501 ctctattcca ttttctctta agctattagc tgttccttct gtagcaacaa aagtatatcc
    34561 taattctttc atatcttttg ctatctctat aaactcatct ttatcatgat tatttacagt
    34621 agcaagtact actcctcttt catcagacat atgtctacct gctgccaaga accctttata
    34681 taaggcttct tctaaatttt ctcctacacc taggacttct cctgtagact tcatttcagg
    34741 tcctagactt acttcaactt tagataattt agacattgag aatactggta cttttactga
    34801 tactaatttt ggctctttgt aaactcctgt accatatcct aaatccttta atttagctcc
    34861 taacatacac ttagtagcca agtcaactat tggtactcca cttactttac ttatatacgg
    34921 aactgttcta ctagctctag ggttaacctc aattatatat aattcatttt gaaattctat
    34981 aaactgtata ttaaccatac caagcacatt aaggtctaaa gctatttttt tagtgtattc
    35041 tactatcttt tcttttattt catcagatat attttgactt ggatacattg ttgtactatc
    35101 tccagagtga actccagctc tttctaaatg ttccattata ccaggtatca atatatcttc
    35161 cttatcacat atagcatcta cttctatttc tctaccagtt agatatttat ctataagtac
    35221 tggattttta tggtctctat caaatgcatc ttgtaggtat tgagataatt tttcttcgtt
    35281 ataagttatt tccatacctt gaccaccaag tacataagat ggtctaacta atactggata
    35341 acctaactca ttagcaactt caataccttc gttaagtgac catacaccct ttccttttgg
    35401 tctatttata tctagtctct ctagtaaatc atcaaatttt tctctatctt ctgctgcatc
    35461 tatatcatca aaatcagttc ccagtatagg tatatttttt tcatgtaaaa atttagccaa
    35521 ttttatagct gtttgtccac caaactgtag tattacacct tctggttttt ctttttctat
    35581 tatacttaaa acttcttctt ctgttaatgg ttcaaaatac agcttatctg atgtatcaaa
    35641 gtctgtactt acagtttcag ggttattgtt tatgattata gtttctatgt ccatttttct
    35701 taaagatttt acacagtgaa ctgaacaata gtcaaattct ataccttgac ctattcttat
    35761 tggaccagaa cctaatacta tcaccttttt cttatcacta acaactacct catcatattg
    35821 ctcataagtt gaataatagt aaggagatat agcatctatc tcacctgcac aagtatcaac
    35881 cattttatat gctggtttga tattgtatag acttctcaat tcatataatt tttctggact
    35941 tatcttcatt aggtcagata tacctttatc agagaatccc tttttcttta attcaagaag
    36001 atagtcttta gttaaatctt caattttcat aacttttaat ttttcttctt gctctactat
    36061 ccatttaaat ttatttataa accatttatc tatacctgta atttgttcta tcatttctac
    36121 tttatagcct cttcttatca tttcagctaa atcaaatagt ctctcatcat caggtactac
    36181 aactcttttc tttaattcct caatgcttct ctcctcagat ggtgtgtgaa ctaatgaata
    36241 ttttcctgtt tcaagtgatc ttatcccttt aagaatagca gcctcaaaat tacttcctat
    36301 actcataatt tcaccagttg ccatcatctt agttcctaat tttctatttg cttttttaaa
    36361 tttatcaaaa ggccattttg gtatttttgc caccacataa tctatagttg gttcaaaaca
    36421 agcataagtt tttttagtta ctgcattttc tatctcatct aaagtataac ctaaagctat
    36481 ttttgatgaa actttagcta ttggatatcc tgtagctttt gatgctaaag ctgatgacct
    36541 actaactctt ggatttatct ctataactgc atattcaaaa ctatgtggat taagtgcaaa
    36601 ttgaacgtta caaccacctt gaacttctac tgcatttaat atatctaaag atgctttttt
    36661 aagcattgca cattctttgt tacttaaagt ttgagttgga gcaacaacta tactgtctcc
    36721 tgtatgaact ccgactgggt ctatattttc catgttacat acaactatac aatttccctt
    36781 tgaatctctt attacctcat attctatttc tttccaacct tttatactct tttcaattaa
    36841 tacttgtcca acaggactta attgtagacc atgtgaaagt atttctctta attcttcctc
    36901 attatcagct atacccccac cagttccacc tagtgtgtat gctggtctta caactactgg
    36961 ataaccaatt gtaagtgcat aatcaagacc tgcttgtaaa tctgtaacta tatcactaac
    37021 tattacaggc tgatttattt ctttcattac ttctctaaat atatctctat cttcaccttt
    37081 tttgatagat tctactgaag tacctattat ttttactcca tatttatcta gtatgccttt
    37141 ttcataaagt tcaactgcta ggtttagccc tgtttgtcca cccatacctg caagtaaact
    37201 gtctggtctt tctttttcta ttattttttc tataaattct attgttaatg gttctatata
    37261 tattttatct gccacttctt tatcagtcat tatagtggct ggattactat ttattaatac
    37321 aacttcaatc ccttcctctt tcaaagactg gcaagcttgt gttcctgaat agtcaaactc
    37381 tgcagcttga cctattatta ttggtcctga ccctaatact aaagttttct ttatactatc
    37441 taatttaggc ataaattttt ctccttatct aaatggtttt tactatctgt aaataatact
    37501 aaatctatat taatgctaag aatttattaa atatacaatc taaatcttct tttgttgtgt
    37561 attctggaat gtactgaaca ctatatattg gtaattcctt atgtctcatt ccttctatag
    37621 tattgtcatt taaatttatg tgagttactt ctgtattttc tggcatttct gaaacattat
    37681 atccatgatt ttgagaagtt ataaatacct ttccagtttc taaacattta actgggtgat
    37741 tacctcctct atgtccaaat acaagctttg aagtctttcc tcctaatgcc aatgctaaaa
    37801 tttgatgtcc taaacctatt cctgtcaatg gctttttgcc tattaatttt tttatatttt
    37861 caactatatc atttatatcc tctggatttc ctgggccatt agataataag attaagtcag
    37921 gatttatatt taaaatttct tctactgtag tcattgctgg tactatagtt atatcacagt
    37981 tacaagcctt aagagactct agtacactat ttttaacacc aaagtctata actacaactt
    38041 tagctccagt accttttata ttttcaattt cttttcttgt aacatttttt actgaatctt
    38101 tgttacaatg ctcttctaat ttagactgaa catcttgtaa tgatacattt tctaaagtta
    38161 taattcctct cttagttcca ttgtttctaa taattttagt taatgctctt gtatctattc
    38221 cttcaagtcc tattacctta ttttgcttta gatatgtgtc tatatccatt tcacatctaa
    38281 aattatttgg gttatcactt ttttctctta caataagtcc ttttacttgc accttatttg
    38341 attcaacatc ttctaaattt attccatagt ttcctataag tggataagtc atagtaacta
    38401 tttgtccata ataagttgca tctgtcaata gttctccata cccagccatt gaagtattaa
    38461 atacaacttc tccaatagct tcctttagat atccaaatgc ttttccttca aatactgttc
    38521 catcttctaa aattaatttc cccttcatgt aagctacctc ccaaatattt tatctaagca
    38581 ttttctattt ctcttacaat attaatagct gattctcttg gattttctgc ttttgttata
    38641 ggtcttccaa caactaaata atgagcacct ttttttattg catctgatgg agttacaact
    38701 cttttttggt ctccaacctc tgctgattta ggtcttattc ctgggcaaac tgttataaaa
    38761 tcagttccac atgcttctac tattttatct acttcttgcg ctgaacaaac aaccccatct
    38821 attcctgatt cttttgccag tttagctctc tttatagcta actcttctgt tgtcatatta
    38881 catcctatgt cctgtaaatc ttggttgctt aatgaagtta aaactgttac tccaactact
    38941 aatggctttt ctatatttaa tctttctgct tcttccctag caattagtgc acattgtctc
    39001 attccttcca tatctgatac atgtattgtc attaaccaaa cattatctct cactgcagct
    39061 ttcactgcac ttttcatagt atttggtata tcatggaatt taaggtctaa aaatattttt
    39121 ttaccttttt ctttaagata atctattgtt ttaccttttg tggcaacgta ttgctctagc
    39181 cctaccttaa atatgtctac actatcttct aatctgtcta ttagttcttt agctttatca
    39241 aattcgtcag tatcaattgc aactatcaat ttttctttac catttatcat ttttacatct
    39301 cctttttaat aaagcctaaa taatcataaa aaaatcctca caccaaaaga ggcataagga
    39361 ttttatctat acataactct attcattagc tatgagatat ctttcccagt ctctctggac
    39421 tagtttaaaa gactttatta tatttaaatt tttattttat actcatcaaa aacctctaga
    39481 agatttacta gaggttatat actaacatta tctataaccc tttagtctct ctggactact
    39541 ttaaggaatt tagctgtaat atttttcttt atttatataa ctttatgtat ctataggata
    39601 atttaaagtt ttatcttttt tataattaaa ccactaattg ctttttatgt caataagtct
    39661 ttgaattatt cttcatcttt atcaaacaat tttttctcaa aatatgattc tgcattatat
    39721 attgcagcaa ctggattatg cccagttact ctatctttga caactaaagt tgttatagga
    39781 gcttccatat atttcatggc taaagtatcg tgaccaacac ataatccaag aattacatta
    39841 aggtcaacct tagcttcatt taaaaataaa gcttgtccta ttggattgca cattacctca
    39901 tctgaacact ttgatattgt ttgagatgct tctagtccaa ttgtactctt aggcattgcc
    39961 ccatttttac atataacaga aaatacttca aacccatgat gttcaaatat tttttgcact
    40021 gtttgcattt ctctttttag tccaatacaa aatgctatgc caatcttttt ataaccacac
    40081 tttttagcaa actctataat ctcttttagt ctagtatatt gacaataccc ctctgcttca
    40141 actaatccag cattataagc tatttttcta ttctcttctt cttcataaag ttgctttgct
    40201 ttttgttgta gttcaatttc tctagttgga catatcttca ttaatttatc catatttcca
    40261 ttagaacagc ctctaagatt acattttcca cacttataca tatccaatat ccccttccaa
    40321 aattttattc atcttgcgat tcttcttttt ctagagttac attttcttct tgggtttctt
    40381 cttctggttt cacatgctcg actacagtta taaaatatga ttttcctatt ttattcctta
    40441 taggaagttt ttcatatgaa acatctattt tattttttat acaagctata tcaaaatgtc
    40501 ttaaggctac acctaagcta accttattca tatcttcaca gtgtttctta ggtctctttt
    40561 caaataaatg aagacctcgt tcatccttat ggaaatacca cggttgagaa ttttttacag
    40621 atggagctct tcttgtcaat tctaagatat ctttccactt tctattccta tttatcttat
    40681 ttatcatatc ttttactggc tttctatcag gtctagcata tctcgtccta aacaactctt
    40741 cattttttgc tggatatcca aatgctatta ttatataagg attttctatt ttgttttcat
    40801 catcttcttc tgtttttatt ccagctagat aatctaaatc atcttcctca tcttcttcaa
    40861 aattcttatt cccaatatct acaaattcca agatatcttc tctctttaag tttccttcca
    40921 accagcaagt agctattccc aatgttgtaa ggcgcaaaac tacaccttct gtagcaaatc
    40981 ctatattctg taaatagtcc tcacctttgt ctgatgtaac tactatataa tgaggtgcct
    41041 ttactttaca ttccttaccc atcaaaaaat gtattatatg acctctatca acaacatgtg
    41101 ctttaatatt taaatcttca tttaagtatt ctaaattttt acaacttctt tttatttctt
    41161 ccatcaatgg cttttttatc tttttactgg aaaaatttct tacagatttt ctataaaaaa
    41221 tggcttcata aagctccatt tatgccctcc taactgaaat cttctgacgt ataaactcta
    41281 taaatttata taaatacttt atatttttta tagttttatt tcctttttcc tcctgcccta
    41341 ttttgaaatt cataagaata tctactctag tttaatatag cattcaaaag gcttaaattc
    41401 taccttatca atgtctatac attagcataa tttttttatt tgattttcta tgctaattta
    41461 aataacaaca ctagctatta actttttaat taagctttgc cagaaaaatt ttttttcatt
    41521 tataaatatc tatctcatta ttatattata agtttttttc ggtattcttg tatagctttt
    41581 cactaaaaaa gtatttttat ttctacttta atattattat caacaaaaaa catactgctt
    41641 aaataagttt gttatcctca taatctgttt taattaaaat atttttttta tttctcaatc
    41701 tataaagtat aaatattttg aatatgaccc aaaatctaag taaagttata atattttata
    41761 agttgttcaa tattaaaaac ataagtaatg ttataatact ttataagtta ttcaatatta
    41821 aaaatatagg aggataaata tgaatttaaa tagaaattta gaaaaatacg ctgaattagc
    41881 aataaaagtt ggagttaaca tacagccagg tcaaattcta cttataaagt caccaataga
    41941 atgtgctgat tttgcaagaa atgcacttaa agaagcatat aaatgtggtg ctaaaaatgt
    42001 ttatatagag tggtctgatg aagaaagtac tcttattaaa tacttatatg ctccagatga
    42061 agcatttcat gagttcccca aatggacagc agaacaatat gtagatatag caaaagaagg
    42121 tggggcattt ttatcagtct atgcccaaaa tccagattta ttaaaagatg ttgaccctga
    42181 aagagttgca aattttcaga aggcatctgg aaaagcttta aaagaatggc gttcttacac
    42241 tctaactgat aaatgtaaat ggagtatagt ctctgtgcca acaaaagatt gggcaaaaaa
    42301 agtgtttcca aatttatcag aagataaagc aatagaaaaa ctgtgggatg ctatatttaa
    42361 atgttcaaga gtagatggtc aggaccctat aaaagcatgg gaagaacata atgaaaatct
    42421 taaatctaaa atggactttc taaataaaaa taactttaaa actttaaaat ataaatcctc
    42481 aaaaactgat ttgactctag acttgccaaa gggtcatgtt tggctaagtg gagcatccaa
    42541 agaccctaat ggaataagtt ttaatccgaa tattccaact gaagagattt ttggaatgcc
    42601 acataagttt aaagtaaatg gaactgttta tagtacaaaa cctcttgttt atggtggaaa
    42661 cataattgac aatttcttct taacatttaa agatggtaaa ataatagatt tctctgctga
    42721 aaagggactc gatacactag aaaaacttat tgaaacggat gaaggctctc attatttagg
    42781 agaagtcgct cttgttccat acaactcacc aatatccaat acggatataa ttttttataa
    42841 tactctatat gatgaaaatg cttcttgcca ctttgcaatt ggttctgctt ataagacttg
    42901 tctggaaggt ggaaataatt taaaagatga agaattagat gaacatggtg taaatgacag
    42961 tttaacacat gtagatttta tgattggttc tgctgatatg gatattattg gtgagactta
    43021 tgatggaaag caaatacaga tttttaaaaa tggtaattgg gctttttaac atatgatttt
    43081 aactaaaaag ggctgatgga agttatacca gacttcccag ccctttttaa tgttgatgat
    43141 aaatattttt tatattaatt aatctcatta ttatagatta attcctattg ttttaaacat
    43201 aagtatctaa aacttatatt tatattatat ataaaatatc ctatttagta aaacctttat
    43261 tttaatttat atgctaaaaa tttaactttg ctatttcaac agcagacttt gcatcctttg
    43321 aataataatc agcacccatt tcttcagcgt attcttctgt caaaactgca cctcctacaa
    43381 atacctttgc atttatctca ttatctctca gtgcctgaat tgtatctttc atactttgaa
    43441 cagtcgtagt cataagtgca cttaatccta ctaattttat atttctcttt ttagcaactt
    43501 ccactacttc ttcaataggg acatctttac ctaaatctat aacttcatat ccataatttt
    43561 ctagcattat ttttactata tttttcccaa tatcatggat atcacctttt actgtagcaa
    43621 ctattatttt tcctttggat acattattgc tacttttact taataaagtt tcctttattg
    43681 tatttaatga aacttttacc gtttctgctg attgaatcaa ttgtggtaaa aatatatctc
    43741 cactatcata tctttttcct actttatcaa gagcaggaat taaaatctca tctaatatat
    43801 aattttcatc cttctctttt aaaagattca gtgttacatt ttttgcttca tcttttaatc
    43861 ctctttcaac taaaatatcc aagggcaaat ctttatcaat ctttgtagat gaatccgatt
    43921 tttttgaatt atttatattg ctatattgat ttatatagtt tatacatcct ttatctgtgt
    43981 tatttaatat tttaaaagaa ttgatagctt ccatcatact atattcattt ggatttataa
    44041 ttgctaaatc aagccctgcc cctaaagcta atgttagaaa agaagcatta agtgctttcc
    44101 tatttggaag accaaatgaa atatttgaaa cacctaatat agtttttact cctagtgtct
    44161 ttaccattgt tatagctttt atagtttcta ttgcttcttc ttgttgtgct gaaacagtta
    44221 atgataaaca gtctataaat atatcttttg gttttatccc ataagaaact gctctattaa
    44281 ctatcctttt tgctatttca aatctttctt cagccttttt aggtatcccc ttttcatcaa
    44341 gtgtaagtcc aaccacacat gaaccatact ttttaactat tggtaatata ctttctaaag
    44401 actcctcttt gccatttaca gagtttacta ttgttctacc attataatat ctaagtcctt
    44461 gctctaaggc ctctacattg gaagaatcaa cttgtaaagg tgtatctact actgcttgaa
    44521 tctctcttat taattttggc attagttttt tttcatcaat ctctggaaga ccaacattta
    44581 ctccaagtat ttgtgctcca gcatttactt gttctaaacc taaatttata gcataatcaa
    44641 cattttcatt ttttaatgct tcttgtagag attttctacc tgtgggattc aatctttctc
    44701 caacaactgt aggcgattgt acttccacaa attttgatgg tgaacagact acacatttat
    44761 caattttctc gatacatccc tttgtaacag aatttatatt ctcagatatt tcttttataa
    44821 aacttggatt cgtaccacaa catccaccta ttatagtagc accaacttca acgaactttt
    44881 taaccccttc aaaaaattct tttgcatcaa catcatatat agcctctcca tcaactatat
    44941 ttggaagacc tgcatttgct tgaaccataa cagggactgt agctcttgat gctatttttt
    45001 caactattgg taaaagttgt ttaggtccta atgaacaatt aactcctatt gcatctacac
    45061 ctagaccttc tatagtagcc accatacatt ctggcataca tccagtaaaa cttcttccac
    45121 cttcatcaaa tgtcatagta cagaaaatag gtaaatcaga gttttctttt gctgctaata
    45181 cagctacttt agcttcatat aaatccatca tagtctctat tactattaca tcaactccag
    45241 atttaacgcc ttgtagtact tgtcttttaa atatttcata tgctttatca aaacttaaag
    45301 tacccatagg ctctagcatt tctcctatag gaccaatatc taaagctaca tatcttggtt
    45361 tagtattatc tacattttct atagcttttc tagccaccaa aacagcattg tcaatcactt
    45421 cttctacagt atacttacta tctaatttta gttcattaca tccaaaagta tttgtaagaa
    45481 ttacattcga acctgcctct aagtatgctg tatgtatttc tataagttta tctggatttt
    45541 gaagaccaaa tacttctgga ttttcaccta actttaaccc tttttgttga agcattgttc
    45601 ccattgcacc atcaaatatt aatatattat ttttcagata ttgtcttatt tccacaagag
    45661 ttcgctcctt ttctatatgt acagtttcta tagtttttac aattttcaca cgacttttct
    45721 ttcttagatt tatttattct tgtattagaa atacctataa tagccactac tgactttctc
    45781 ggtatcataa ttccattaga agtaattgtt agacctattt gtttttgagc attcaaaacg
    45841 tttaatatat ctatattttt ttctattggt aaatctccat atcctgggct atatcgcata
    45901 gttataaatt tatcttgctt taaaaaaacc ttttctaaat tattttgaac aatgtcacac
    45961 acttcttcta ttgctgttgt tccacatgaa tcaattatta gtccttttgt taaatttgaa
    46021 taagagtgtc gtcttatttc tttttctata tctatcccta tagtagctgc cataattaca
    46081 cactcattac attcttttaa aagttcatat acatctttac tctctaatgt taaatttgtt
    46141 ccctctaatt ctacaacatt atctttattt attttaatag gataaatcct agataaatat
    46201 ctaggattta ttttattttt agttatagct acacattcat caatagtata tttaaggtct
    46261 ttatctatct cttgacctct gtattgtaaa tatcttaaaa cttcactttg atttatgctt
    46321 atatcttctc ttccaattaa attattctcc atatatattt aaagaaaatc ctttatctaa
    46381 aacatctttt gcatattcct ttgccccaaa taaagataag tctctgtagt ttccacattc
    46441 tatagctgta gctgctggca ttttatctga ttcaagaact ttttttaatg cttcttctaa
    46501 accaatctta actgttttgg catctacatc tccccatagt attaaataaa atcctgttct
    46561 acatcccatt ggagataagt ctattacacc atctaatgta tctcttaaat aagtagctaa
    46621 aagatgctct aaaccgtgca tagcagcagt tccaaaactt tctacatttg gctgtaaaaa
    46681 tctcaagtca aatttagtta ctttatctcc ctttactcca tcaagtacac tacactttct
    46741 tacaaaagga gcttttactt ttgtatggtc tagcttaaaa ctttctactt tttccattat
    46801 ttaatcctcc attatacttt aaaatttgtt acattaattc taaaaaaaac tcttcctatc
    46861 tattattgat aagaagagta tattatttca agtcatctca tcgttgttag caggaccacc
    46921 tgataaaagg ttggtatagg atcactgagc taactctctc ccctaactct tgataagtat
    46981 cttattaaac tgacttaaat tatatattac ttttttattt ttgtaaacac tttattgtta
    47041 taatatttat atcacataat cgtactttta ttttataact aagctgattg tattattggc
    47101 agttgtactt caaacacagt ttgatattta tcgcttgaaa cagtaatttc tccatcatgt
    47161 aaatttacaa tattttttgt aatcgcaaga cccaatccag aacctccaac atttctattt
    47221 ctcgattttt caactctata aaatctatca aatatatttg gtaaatctaa cactggaatt
    47281 ggttccccat aatttatcac ttttacaacc gccatatctt cctttttctc tgtgactata
    47341 tctacataat gcccatcttt tccatatttc acagcattag ttataagatt ttcaaatgct
    47401 cttaccaatt tatttggatc agcttttaca attaattttt cttcattaaa attaattcta
    47461 ctaaccataa attcttgttt aaaataaatt tcaaattgag atactacttg acttaataac
    47521 tctactaaat ttatttctat tttgtttaat tttattgtat tattttgcat tttagtcaat
    47581 tcaaacaaat catttataag aacatttaaa tttttcgatt tttcatatgc aatatcaaca
    47641 taatacatca tctgaacttc atctttatac ttaccttctt ctatcactcc taaataaccc
    47701 attatggatg tcaaaggtgt tcttaagtca tgtgacacat ttgttattaa atctgttttt
    47761 gtctgctgag cttgtctctc ttcaatagtt atatttctaa gttgatggac tatattattg
    47821 attttatgtg ctagttcatc aatatcatcc ttttctttat cctttaatat atctaagtca
    47881 tactcaatat ctatactttt ttctaagtta ccttcatcca ttaaatttac agcactttta
    47941 atgatttcta atttttgatt ttttctatta gtctttctta taaaatctac aatttgctta
    48001 tttctcttgt acgatatcat ggaataaatt gctaatgatg tgattgtaaa taaaaatgga
    48061 taaaataaat cataccctat aagacttctg aaatctcttg cttgatatac aagttgatta
    48121 actacagaca tatcactatt gacatataat gattttatta tatacatact tgttaaaata
    48181 agcattgaag ttaaagcaca gcttattata agatctaata tgggtcgaaa catatgattt
    48241 aataaacttt tatttttcaa ttttatatcc tactccccat acagtatgaa ttattttttc
    48301 tccatctgta tatttttcaa ttttatccct tattctgctc atatgaacca ttacagtatt
    48361 attagattgg taatatttct ctttccatac cttttcaaat atttcttctg tactatatac
    48421 tctgccttta ttatttgcta ataaatataa tatatcaaat tctcttgatg tcaaaattat
    48481 ttcatttcca gccactgtta cactatgttt ttccttatct ataattacta ctccaacctt
    48541 tatgacatct ttatcttcat tattagcacc actaaaataa tatgctcttc taatcagcga
    48601 cttcactctc acagtaagtt caagtgggtt aaatggtttt gtcaaatagt catctgcacc
    48661 agtcattatc ccttgtattt tatccatgtc ttctgatttt gcacttaaca taagtattgg
    48721 tatattcaaa ctttctctaa cttttctaca aacttctatc ccatccattt taggcatcat
    48781 tatatccaat atcattaaat gaatattttc cttttttaaa atatctagag cctcttttcc
    48841 atcttcagct ttaattacat tatatccact atttaaaaga tatatctcta taaggtctct
    48901 tatttccttt tcatcatcca caacaagaac tgtattattc atttttacac atcctaactt
    48961 aatctttgta tatagcttta ttcatataaa attatactat attttagtta gtgtaatcta
    49021 taaatttatt aatttctaaa tttccaatat aatacaaatt atttacacat ttatattatt
    49081 tattttgtaa atattcttct aaagttaatc cttttgaata aatttcctcg gcagcatcct
    49141 taccaacata tctaatatgc catggttcat atgctgtacc tgttatatct tctttacctt
    49201 caggatatct tattataaat ccaaacttgt gagcattttc tgccaaccat ttagcttctt
    49261 ttgtacttcc aacaaaccat ctttctggat ttgttaaatc aatacaaagt ccagtttggt
    49321 gctcactgta accaggcttt gctacatatg catctgcctt ttctcgtccc tgagatttta
    49381 cccttcttgt ataagtatcc agttgagtat cataagatct gtatgctgaa gaccctaaaa
    49441 actgtattcc ttctgaatta gcttgcttaa ctaaatcttc aactgccttt gcaggttctc
    49501 ctgccatttg tttttcttcc tctgttgctt cttcaacaaa aggtatgttt accttagtaa
    49561 tattttcagg agtatagttt ttgctaattc catttgtctt gtttaccaat aatatatttg
    49621 atatactatt tgagtgattt ttattttgag aatttgcctc aacatcttgt tctgaatcat
    49681 gttgagcagc tactattgga tctttatctt tcttaagtaa tctatcttta gatataaata
    49741 cacttataaa tactactaaa ataatggctc ctattaattt aaattttttc ataattaaat
    49801 cttctccttt ttgttatgtt tgctataatg ttaattttag agtataagac ttaagtattt
    49861 tataagtaaa tcttaaataa atcttaaatt taatattttt aaaggatggt gataaaatgt
    49921 ctatacaaaa acttctaagt aaagctcgta aggctataca agattttgat atgatacaag
    49981 aaaatgataa aattgcggtt ggactttctg gtggaaaaga tagtttaact ttacttcata
    50041 tacttaaaag ttatcaaaga ttttctccac aaaactttga attgatagcc ataacattaa
    50101 atactggagg tgttgataat tctccactag ataaattgtg taaagaaata aatgttcctt
    50161 tttatgaatt tcaaactgac ataaaagaaa ttgtatttga tataagacaa gaaaagaatc
    50221 cttgtgcatt atgtgctaat ttaagaagag gtgctttgaa cgataatgct aaaaaacttg
    50281 gatgtaataa agtagctctt ggtcatcata aagatgatgc aattgagaca tttttaatgt
    50341 ctatgtttta tgagggtaga gtaaattgtt tttccccaaa gacataccta gacagacagg
    50401 atttaacagt tataagacct atggtttaca tagaagagta tatgactaag aaaatttcaa
    50461 aagattctaa ctatcctatt atcacaaatc cctgccctgc aaatggtcat actaggagag
    50521 agtacataaa aaatcttatt gctaatttaa ataaagagat gcctgatttt aaaagaaatg
    50581 tatttggtgc tttaaataat tctgagaaat tgtttatatg ggataaagag aagataaaaa
    50641 attttaaata ataattaaaa tttttaaata aaaaagcaag attgtattaa ttgtaaataa
    50701 tctttatata atctacacaa tcttactttt ttatttatta tatgttatta tctttaatct
    50761 atattttaat agttcttaat agtatttatt ttgtctaata cattaattat aacttaaata
    50821 ttttatatat taatttaatc tattaattca ttcaatctac tggtttaatc catttttaca
    50881 gtatatacat ctacatatta taaaaagcaa aattatattt tctaaatatt ttatgttaga
    50941 ttataatatt aaattctatt atctctggta tattaaaaaa tctaaatggt aactttgtac
    51001 ttccaagacc cgaactcaca tataaatgat ttccatttat atcataaaac cctttagtat
    51061 atttccttcc atatggagga gtaactaatg ctcccttaat aggtaatctt atttgaccac
    51121 catgactatg cccagataga actaaattca ttgtatcttt actaagcata tctactaaat
    51181 ctggttcatg tgctaacaat aaattaaaac ttttattatc tatttgactc tccagatatt
    51241 ttatattagg atttccattt aatatttcat caacacctaa tatattaata tatttatcat
    51301 cttttaattt tattttttta ttttcattta ctaaaagatt gaagttggaa tttttcataa
    51361 tgtgcctata atacctagga agtttataca tataatcatg attgccaaat actgaaaatt
    51421 ttcccaacct agcattcata cttgataaaa tagttgtaat ttcatctaca cttggatttt
    51481 tattggagta atcaattaaa tcaccagtaa atactattat atctggattc aaagtgttaa
    51541 tcttattagc tacttttttg agattcttag ttgaataaaa actacctatt tgagtatctg
    51601 aaatttgaac tacttttaca ctttcagatt tactctcaat tgtatcattt tttatcaatg
    51661 ctccattttc acttatatta tattttttca caattaatag actcggctct atcgcaactg
    51721 agtatataaa aatcaaaata aatattaaga taaaaaattt taaaaaatac ttaatttttg
    51781 agttcaaaat atctacctac ttatttattt ttttaaacta tttgcattac agactcaact
    51841 ttgttcattt ttctgtcatg catttttggc ttatttgctg aaaaccattt atatgttgct
    51901 gtaagacctt cttttagtaa cacgtttggt atataaagcc catgttcaat aagcttattt
    51961 atatcaagat taaagtctat atttctaaat gggaaatacg ttctttctct aaactctact
    52021 ttttccatat ctacatattt tattataggt tctttgccaa taatttctcc acatgtatat
    52081 ataaggtcat cccaagaaat taactgtgga tttgtcacat tataggcctc tcttacatga
    52141 ggatttttca ttatgctttc caataccttt accaaatccc caatatatat aaattgatta
    52201 ctaacttgtt ttccttttgg tactggtatc ttttcatttt tttctatttt ttcaaagaaa
    52261 taatactctc tatataaatt attattttca ccatatatgt aagttggtct aataattata
    52321 tatggtatag ggctatttat aataaaatct tcagcctctt ttttatttaa cccatatttt
    52381 ccccagtttt cattttcacc tttctcattt tcttctttta tatttcttcc actatcttta
    52441 tatactgctc ctgctgacaa tactatatac ttttttaagt tatccatgct tataaaatca
    52501 atcagattac ttacatcttc ttttgtatat gctgtcatat catatatgta atcatatttt
    52561 cttcctgtga taatgttttc catatctttt cgaacttttc tatcacaaat tagatgttct
    52621 ttaaaaccat tataatctat ttccttttta ccatttgtaa gtatatcaat ttgataccca
    52681 catttaatca agcgcttagc aagtgcagaa cctatgaagt cgcttccacc cataattaaa
    52741 atagatttca ttttaagtgc atcctcctct tttttaaatg acttccttaa ctataatatc
    52801 atatttttaa atatgcgaca ccattttgta atatttttgt ctataaaaga tatttataac
    52861 aaaatatctt ttatagactc tacattttcg acaatataag taggttcact ttcactaatc
    52921 tcttcaaaag aaccataccc atacaatact ccaatagaat ctatacctat ttttttagct
    52981 ccaattatgt catactttct atctccaacc ataataactt tatctttatc ttttacatta
    53041 cataggtcta gtacatattg tataacttca tttttattca ctcttgtacc atcaagattg
    53101 ctacctgcta tatatttaaa atatctatct atatcaaaat atctaagtat agtttctgca
    53161 aaaactgttg gcttagaagt tgctactaat aatattttac catttttata gagcatttct
    53221 aatatctctt tcatattctc ataaatttta ttttcaaata tccctttatc agcaaaatat
    53281 tctctatact tttcaacagc ttctttagct tttttatctt caaatttata atactccttg
    53341 aaagtatcat gtaatggtgg tcctataaat tggtctaagt tttccaaatc ttcttttatg
    53401 ccaaagctat ttaaagaata ctgaatagac tttgttattc cttctttagg gtctgtaagt
    53461 gttccatcta aatcgaataa tactatttca taattttttt tcatatttca gtcctccata
    53521 tttgtaaaaa gtaatatctt atatattaag tttatctatt ttaaaaagct atagtaatag
    53581 aaatttctaa tactatagct taagtaccaa ttaaatctta tatatttatt ttaaaaataa
    53641 atttaaacag ctctatttaa ctgttatagc tgaaactgga catccttctt cggcttcttt
    53701 acataaatct tgtaaatctt ctggtatttc tccttcaatt gctactgaat gcccttcatc
    53761 gctcatacta aaaacgtctg gacatgttcc tatacatgct ccacaagcta cacatacatc
    53821 cttatcaaca aatgctttca tactaaaata cctcccctaa aattattatt ttttaattgt
    53881 tgtttcctta gatatttatc caatatatac caatttaaac atatatttat tattttatca
    53941 ctattgttta gtttattata gtattttcgt atccatgtct ataaacttca ctgcttttca
    54001 tccatgattt tacagtatat tgcaatctta attttttatt taaaacaagt tcattattat
    54061 tgtagtcttt tataatatca ttacactcct ccattgtaaa tattgtaaaa tcaccttctc
    54121 caccaatctt tattctgcct ttatttccat gtaaatcgaa aatctttgtt gggtattcag
    54181 ttactttttc tatacacttt tctagggata tcccaagttc cataatttta tttataacat
    54241 ttaaaagaga atagtttacg tctttcatgt tttttatatg catatcagta cttattaggt
    54301 caggctctaa accttgcttt attgcttttt taaaaacttt aaaatcaaat tggtccactc
    54361 catgacatac atcaaacaat actcctctat tctttgcatt tattactgat tttcttaatg
    54421 tgccagattc tttaaggatt ccatttgtac tatttccata tactggtgta accacatctc
    54481 cttttcccaa aatattcatt acttcatcta tatatgatgg atactctcct atatatacca
    54541 ctaagggtaa atttattttt ttagcaactt gctttgattt cttaattata tctataccac
    54601 tttcattaga ttgaaattta ctcgctttta cttttatacc ctttatcttt tctatatttt
    54661 tttctacaat tgactcaacc ttgtcaaaat ttataacatc taaagaagtt gattcattca
    54721 atatatttag tccctcattt gtaacattta aaaaagaata tacttctgtt ttacactcat
    54781 ccatatactt ttttttaaaa tcatagtagt ttgaaggacc agctgtacct gcatcaaata
    54841 taactgtaga acctttttca attccaattg aatcagaatc catacctgta ggtaattttc
    54901 tcttataaca atgggtgtga acatctataa atcctgggga aatatacata ttatcggcat
    54961 ttattatctc gaactcttta tttgtaagta atttttcttt tcttattttc tctttaaggt
    55021 tcaatccaat agcatatatt ttcccatgta aaaccaagat atctcttttt tcaaatttga
    55081 taccatctgt tggagatatt attgtcccat ttcttataag taaatttttc atttattcca
    55141 catcctaatt acaatataat ttctctaata gcttttctta cataaagaaa ataagcctaa
    55201 aaataaaaat attttagact tattcactat attatactat attaattata ttaaattaaa
    55261 taaaatcttt aatttacttt aagaagcaat taagtatttg ttgctcggct tcttcttcag
    55321 taagacctaa tgtcatcatc tttagtattt ggtctcctgc aatctttcca atagctgctt
    55381 catgtactaa aagagcatct ggatgatttg cagcaatttc aggtatagca ctaatttgag
    55441 aatttcccat aattattgaa tcacattgaa catgtgcaga acaaagggaa tccccaataa
    55501 cacaaggatt aaacttttgc ttagaattat cttgcccaac tgtacgagaa actacttgag
    55561 cagaagagtt ttcaccctct aagataacct tcatattaga ttctgcaact tgattaccat
    55621 gagttaataa tttctcaaat acaactattt tagctccttt acctaggttt gcatccattt
    55681 ctcttttagt tgaatctaca cccttaattt gaattgtttc catttcacaa tatgaatcct
    55741 catccatatt gacaatagta actggattta aaattctctt tccttctcca tcaccttcac
    55801 cataatgttt ttctacatat tttaggcgag ctcctttacg taagaaaaat ctatgtatac
    55861 cattatgttc actatcatca caaccacagt tatgtatacc acacccagca attataacaa
    55921 catctgcatt ttctccaatt tcaaaatcat tatataccat atcctgtaca ccagcctctg
    55981 ttactattac tggtatatgt acattttctc cttttgtatt tggctttact ataatatcta
    56041 ttccaggttt atcagtttta ggaattatag ttacattttc agttgaatgt ctctctactc
    56101 cttgcccatt tttacgtata ttgtatgctc ctaatggagt tccacgaagt tcagcaactt
    56161 tttctaaaag atttaaatct attttgttca tttaatttgc cttcctttct aattaattca
    56221 tgtctttcat taattcacaa cttgaattaa cttgacacat gatttcaggt aatatagcct
    56281 cttttgttcc atggcttttt actgaaccat cttgtagaac aattatttca tcagctaact
    56341 ctaatattct ttcttggtga gaaattatga ttatagtctg attagactct tcatgtattt
    56401 tcttaaaact ttcattgagt tttccaaagc tccaaaggtc tatccctgct tcaggttcat
    56461 caaatataga aacctttaaa tctctagcaa aaagagttgc tatctcaatt cttttcattt
    56521 cccctccaga aagagagtta tcaacttcac gatttaaata gtcttttgaa cacaatccaa
    56581 catctgtaag gtattggcaa catacatcag tagacaatgg ttttccatga gcaagtgtaa
    56641 gtaaattttc aactgtcata ccttttattc ttggtggttg ttgaaatgcg tatcctattc
    56701 ctttttttgc tctttctgta atactaagat ttgttatatc ttctccatct aaaattatct
    56761 gaccactcgt agctttttca attcccataa taagcttagc aattgtagat tttcctccac
    56821 catttggacc tgtgataaca ataagttttc ccctctcaaa tgaaaggcta acatcattta
    56881 ttattcctag ttcctcattg ttagactcaa cattaaagga aagatttttt atttcaagca
    56941 taatacccct cttttctata aaatattaca ttaatatcca ttcttttatt atatcagtaa
    57001 tttttatata tcagcaactc taaggagata aaatgtatat taaataataa aacaaagtta
    57061 ttgaaaagta aaaaagatga gttttatgta aactcatctc agaaatttag tatatcaact
    57121 tatatgtatt taaaatttaa ccttctatat actctaatat tattttttga ttatctttca
    57181 acataatttc ttctttttta gattctattc cattcttttt tatctttacc tcagctacaa
    57241 aggcatcatt accttcaacc atttctaata gttcatacgc ttttttacga cctattttag
    57301 ttgctatatc ttttagtact gccattttct taaacatttt atcacctcta ctcaaaattt
    57361 acacttaatt tttaattata taattctaat cctagactag tttataataa ttattgtctc
    57421 taattatatc agatattgag taaatttaat actttgtaaa attatttatc ctctctgacc
    57481 aaataatcat ttccatcttg gtctttaaaa gaaaacatcc ttccatatgg catctccatt
    57541 aattccccaa cctcaacctc gttatttgcc atttgttcat aagttccatt aatatctctt
    57601 gtacttaaaa ttattgatgg atgagataca tttatctctt tgttttgctt catcattaga
    57661 tttttatcat acaacacaaa tgttgtaaat tcatttttac ttggtgctac ttccacccat
    57721 ttcatatttg gacccatttc cttttcataa gttactacaa agttcaattt ttctgtccaa
    57781 aataactttg catcctcttg attatttaca tagagagtta tttttcctat tttatttatc
    57841 ataacaatac ccacctttat tattaaataa atacctaata ttttttatat ttaataatat
    57901 acccatatat attattttct atagatatat atttctaata tttatcgcca aattatgtaa
    57961 ttctgctcaa tatataattt ataatatcca tactattata gcctttattt atgtaattta
    58021 atcaccttat atatcatact tataattatg ataataacta cataaaaaat cacttctaaa
    58081 tagagttatc catctaaaag tgatttcata tattctatgc aactttgtgt tttagccact
    58141 ttgttcctct taatcttaaa caatataaag cccctctaac aagccaatca gtatacatac
    58201 ctatccaaat tcctaacact ccaaattcta atacaacccc caatacgtaa cctgtaaaaa
    58261 ttctaaaaat ccacatacct attactgcag tcatcattgt atatctagta tcaccggctc
    58321 cctttaaccc tgctgataat acaaaggata taggccatat taccatagct aaacaatcac
    58381 ttcgaattaa ttgtgttgag atgtcaatta cactagcatt ttgagtatac atacttgaaa
    58441 tccagggtgc aattggtata aatatagctc ctactataaa taaacaaatt gttgcaaatt
    58501 tagttaaata taataaagta ctcttagctc ctttaatatc tccacgtcct atatactgtc
    58561 ccaccaaggt tgtagcagac aaagctaaag cattcccaat tacatttatt atggatgcaa
    58621 ttgacattcc tattgcattg gatgcaattg ctgatgttcc catagtaact ataaatactt
    58681 gaacaatcaa tttacctgca ttaaaaagta gttgttcaac tccagctggt attcctatat
    58741 taaatataga cttttgagtt tcaacatcaa tcttaaatgg aaatatttta cccatagata
    58801 taaattcgtt tcctttaaat aaagctctta taattactat acatccaatt attctagcta
    58861 tagcaataga tatagctgca cctgttatgc cataggatgg tgtttgcaaa tttattccta
    58921 gaggatttaa atcaatacca tatataagta tatatcccaa tgttacattt actatattca
    58981 taaacatagt cacatacata ggtttcttta catctccaca ccctcttaaa ataccatttg
    59041 ctatttgttg tgtcgcaata aatggatacg taaataatgt aaactctatg tataattttg
    59101 catttgattt taccaattct tctgctgaac cataaaataa attaattaca ccagttctaa
    59161 aaacccacat caatattgtt attgtaaagg ataatattac tccagacact agagcctgct
    59221 taatagtttc attcaccttt tcactatctt tttttcctat ttgttgagct acaacaacag
    59281 tagctccaac agatagtgca gcaaatacag atataaatat attgtttatt gaatcaacca
    59341 ttccaactgc agatacagct tcttccccaa tatgacctgc catcattgta ttaaatactc
    59401 ctaaaagcat gacaaacgtc tgctctatca ttataggaat tgctaacttt ataacatttt
    59461 tccttataaa catccctatt cctcttttcc ctctttatat aaatttaatt tttaattagt
    59521 ttatcgaact gtaaattgac aatgcaaagt ttagaattgt atcgttcgca ttataatata
    59581 ctgctttcaa ataatataaa tcttgtttaa tatcatttga acacagtata tctttataat
    59641 tatcttatgt ttattatttt ttatggatac cacatttaaa atatttatta atttaatata
    59701 aatattttcc gttattatta aaagtagcta tatttcgtta atattgtatc actatcgtgt
    59761 agcaataaca ataagtacat tttatatatt ttttataata ttatttaatc atccaaactt
    59821 ttaccacttt ttcaatatat aaaatattac actttagatg taacaaaatc gcatccattt
    59881 aaatattgta ttttatagac taattttagg atgtgatact ttggttaatt taaatgcaaa
    59941 taatctaaat gtaaatatgg tttatcctat ttcttccaca gcacccatac gtttcagaag
    60001 atatactgtt tcatatttag aaaacatgga aaatatttat ttaactatag caacatactt
    60061 tgatacactt ggtttaggca caatgaatat cgacaaagtt tatggtcaat gggcttggtt
    60121 tactgataat atatatgatt taaatctatt tgtttttgta ggaaattatc cttatcaaat
    60181 tgctaaaaac agatatgata catttgtaga tattttgcca gtagctatta cttctattgt
    60241 caatggagat agagtatttt taatttcaaa tccttgtttg ttaaattcaa aaattacagt
    60301 tagatttata tctagtcatc ctactcttaa tagaacagtt gattatggca atattagaga
    60361 ttttattttt taaaactgca acatgatgtg ctattaaaaa ataccctaaa tttaatgata
    60421 ttattaaata actagaatat ttttaattgg cacatcatgc ctagctatta taaattcata
    60481 atattatttt acttaagata agtactgtat tttatctact tgtatatttt tatttaccta
    60541 gtttctcagt tctatatcct atttctttat tagctagctt ataaccttct tgtatcattt
    60601 cttctctcgt taatgcactc cctaataaat tgtcaatact cagttgcttt ctatcactaa
    60661 aatacttatc caataatgat tcctctatat cttcctgata tgccaaattt ccttcatgca
    60721 ttatatattt tacatcaaaa ccttctttat ttaatacttt tcccaccaag atacttctat
    60781 gacatcttat aggttcttgc atagctccta ataaaactat cctataaccc atttgacaac
    60841 ccttttttaa tctttctatt ccgtttaaaa aaatatcttc ttttgctact ttctcaaaat
    60901 ctgcataacc ttcttgcgta tatacatctt tattagttct tttagctcca aattcttttc
    60961 ccatgtatat atatataaat ccggaatttc taagagattc gttaaatgcc tctttattgt
    61021 attgaatatt atatttagaa tatggagttt ctctaatgtc aactacacaa tttatgtcat
    61081 atttttttat catattaata agcttatcat atggataatt tgaatgtcct attgcgaata
    61141 tttccatata aacaccctcc tattctttga aattagtatc tatatatgta gatacccgat
    61201 ttacttaaaa ttaaataaag tattacttta aatacccaat atcttttttt cttgatattg
    61261 tatatttttt tactttatat ccttaaaaaa atatacaact ttatatataa aacatcaata
    61321 tttatatata ttttgtatat taaatacaat tatttataaa gtctttaata aaaatttata
    61381 aatagagcat ctttagaaaa ctattataat aaaaaaataa gaggctgtct aaaaatatag
    61441 ataagccttt tttcataatc gtaattataa aaatatactt gatttcatat gaaaaagagt
    61501 gtctcataaa ctaaaatgtt tactttgaga caccaccttt tttattctta tttatacttt
    61561 gtagacatat tataacatgc gtttcactgg tatctgaact tctgtcaacc agtcttcttc
    61621 actctcttta ttccatatac catctatata agattctcta ggatttccaa ccactttata
    61681 tttattgtcc tctatccatt taaatgcatc tgcgtaagtt tttcctaatg ttgaatatgg
    61741 accattgtgt aatatacaaa ctgcattttc aactgcatct aattctttaa attttaatat
    61801 atccgtatct tcctttaaat catttatagc ttgacacatt tcaacatcca tatcagtctc
    61861 tctatattca ccatcatgat atatattaaa acaataatca ggaactgcac atgtacaccc
    61921 tgttttcatc atttcttcag ccattacatt tggaaataga ttaaaaagtt catcataccc
    61981 ctttaacact tttctcatag aagcaacttt aacttttggt aaagacttaa taattacatc
    62041 attcatattg acttctcctt tcaaattcat aaggtaatta cgtagtttaa ttattttata
    62101 ttcttcactt ttaattacat ccaacatttc tttttcttta ttcattaaaa tattttcaac
    62161 ttcttcagga ttttctacag ctttttttat ctcagctaat gataaaccca tttgttttaa
    62221 agttattatc tgatgtaatt taggtaattg ctctgttgta taatacctat attttgtaaa
    62281 ttcatctaca tattctggtt ttaacaaccc tatatcttca taatacctta atgtctttgt
    62341 agttatttta tttattttag aaaacattcc tatagaaaac atctcatcac ctgcgcatta
    62401 gatttgatta atttaagttt aaactttccc cttaggttaa agtcaataca aaataaatta
    62461 taattgtatt aattcttcaa ttctatctcc actcaatacc aaaatatccc ctgtttttat
    62521 aatggttgaa cctttaggta ttaaaacctg ttcctttctt tttatcataa ctattaatat
    62581 atcgtctggt atattagaat ctattatact cttattagcc catttattac cctcatcaac
    62641 atttacttcg ataagtttag agctaatttc tccatcataa tcattaaatg tttttaatac
    62701 aggtgtatta tcatcaacta aatccaactt tctggctaca gttggtatca atgaaccttg
    62761 tattcctact gaaagcaacg ctataaaaaa tattatatgg aatatgtcat tctctatggc
    62821 tactccttga gtaacagcat aaattgcaaa aactactgaa gccgcacctc taagacctac
    62881 ccacgatatg aataattttt ctttatttga gaatttaaat tttgctagaa ctataaatgt
    62941 tgctaatggt cttgcaataa ctatcatgaa tatagatatg gccattgcta taccaaatac
    63001 atttggtaac ttagaaggga aagataacaa tcctaacata aagaataaca ttatttgcat
    63061 aatccatgaa attccatcaa agaaatgaac caaacttttt ttaagaggta tcctactatt
    63121 tcctataata atacctgaaa tgtaaacact taaatatcca tttcctccaa gatactcgct
    63181 aaatgcataa gaaagtattg caatagctgt tataaatata gtataaaatc cttctatctc
    63241 gaagtttgaa tgtcttaaaa tataaacaga taattttgct agtataaatc ctatagctaa
    63301 cccaaaagct atttgtttta ttaccattgg tgcaataagt tctaccccag aattattcat
    63361 caatgtcaaa agaattagtg tcaacatata cgctattggg tcattacttc cactttcaac
    63421 ttctaataat gatgcaagtg aaccctttaa gtttagtttt tgagaacgca acacagcaaa
    63481 cacagatgca gcatctgttg aagctgcaat tgccccaatt aataaacctt caagtaaact
    63541 agttcctatt accatgtggc aaaacatacc tgtaacgcca gccgtaatta ttacacccag
    63601 tgaagacatt aatatagaag gtatagcaac aggtttagcc atattccagt ttgttccaaa
    63661 ccctccaaaa aacattataa atattagtcc aatagaacat aattgttttg tcagttcata
    63721 gttatcaaaa tagattccta ccatcccatc agacccaaat aacatcccta gtaagataaa
    63781 aatcaataat attggtactc caaatttata caataccttg ctagaagtta tacaaatcaa
    63841 tagtactaac ccacttataa tcattaattc tgtcatagca tcaactccat ttcatataaa
    63901 ttttgtagat taatttacat ctacaatttt attacttatt caaatataac atgacataaa
    63961 ttttataagt tatggtacat aaagttcaac aaaaatttca caatacatta aaatgtgagt
    64021 gttatactta aatttatata tttgttttaa ttatattcat ttaatattta taatttgaat
    64081 ttttttttat taatatttca ttttttagtt gattgtttaa tatatttcat tttttatagt
    64141 aattttatat ataactac
//
LOCUS       DAELNY010000004        57337 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.004, whole genome shotgun sequence.
ACCESSION   DAELNY010000004 DAELNY010000000
VERSION     DAELNY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.004"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            487..3534
                     /locus_tag="KPE55_000201"
     CDS             487..3534
                     /locus_tag="KPE55_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0491307.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVGI
                     VEQVMNDGSISSTFNEGHLTVEGKDAYCIDINTAFKNGYKTRTDASSRMSADRISDVA
                     LSIEYVKQYTDSHSGISSKHAYLLKQLVVWQRLSVQLDWQCDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNAKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLATLTTDTSGNTEAVEVRATTVYIKELSAPAGFKIDKT
                     VYSLSVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKSNSQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLANAYKVSGDSFYMQDGKNVLPL
                     GTLTVEETKSPSGYLLEGAYMQADGSEEQIKEMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKNTAFEIISLNENAVLVESKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALIYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGNDVTIIDTVKLDGLVKGTKYQLK
                     GWQMLKKENAELLVGDKRVESDYTFVADDEAMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPEEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGKKEIEAGKDVTIVDTVK
                     LEGLEVGTKYQLVGWQMLKEENAELIINDKKVENDYTFTSDSETMEVKIAFTFDASSL
                     GGKQFVTFEELYDLSNPDKPIKVTEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDT
                     PTKTDSPKTGDNTNILAFAIMMFVSAGGLAGTYFFKRRKMKKS"
     gene            3756..4079
                     /locus_tag="KPE55_000202"
     CDS             3756..4079
                     /locus_tag="KPE55_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0491308.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQHINGRYTPVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            4094..4507
                     /locus_tag="KPE55_000203"
     CDS             4094..4507
                     /locus_tag="KPE55_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0491309.1"
                     /translation="MRLANGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGADVDWYIKAD
                     DIILKNKGNQPAGNPQNNNPQNLAPQGQQKNKLAN"
     gene            4588..5046
                     /locus_tag="KPE55_000204"
     CDS             4588..5046
                     /locus_tag="KPE55_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0491310.1"
                     /translation="MKFKNPMLVVTDIDKSVEFYKKVFGLRVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGKNFEVYFEEDDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            5110..5523
                     /locus_tag="KPE55_000205"
     CDS             5110..5523
                     /locus_tag="KPE55_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0491311.1"
                     /translation="MLDKIPNAEEMTALVGQSLYAIWNKLCALIDEKYDMECLWNKGG
                     KAWTYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTDRNSYSKEVQKIYDET
                     KTYHDGKWLMFEPTDTILFNDFVKLLGIKRKPNRK"
     gene            5578..6180
                     /locus_tag="KPE55_000206"
     CDS             5578..6180
                     /locus_tag="KPE55_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0491312.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            6241..6783
                     /locus_tag="KPE55_000207"
     CDS             6241..6783
                     /locus_tag="KPE55_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0491313.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKQRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            6811..7122
                     /locus_tag="KPE55_000208"
                     /pseudo
     CDS             6811..7122
                     /locus_tag="KPE55_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
     gene            7233..8627
                     /locus_tag="KPE55_000209"
     CDS             7233..8627
                     /locus_tag="KPE55_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491314.1"
                     /translation="MRIIWNKGHRIRASDKNLVYRFSLGTLLIVFVVVLLLLNMKQLM
                     RTDWEHFSLLDNSFTLSLYNFITLLIATGVCALVAFLYYRFCYDNFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIKYILLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKQHPNYKTGENYAYLSLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSIISEFYTPLVPKGYDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            8829..10028
                     /locus_tag="KPE55_000210"
     CDS             8829..10028
                     /locus_tag="KPE55_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0491315.1"
                     /translation="MNETDFLTALKAKRCAYSVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKNILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERNWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAEQTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPKPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHHQIIKQQTATVEE
                     MVAKVKE"
     gene            10035..10169
                     /locus_tag="KPE55_000211"
     CDS             10035..10169
                     /locus_tag="KPE55_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG0491316.1"
                     /translation="MWELLKDFLLVSLGMGIGVVLMCILTVGKEADRCMEEIKEREEH
                     "
     gene            10911..12821
                     /gene="ltrA"
                     /locus_tag="KPE55_000212"
     CDS             10911..12821
                     /gene="ltrA"
                     /locus_tag="KPE55_000212"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG0491317.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGTPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMPDGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWHENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKTKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKTQKDKLVSQAKKI
                     ASPSKGKTELEEIRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGASKKG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYSANCYTPEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTRAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            12988..13149
                     /locus_tag="KPE55_000213"
     CDS             12988..13149
                     /locus_tag="KPE55_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491318.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            13230..13607
                     /locus_tag="KPE55_000214"
     CDS             13230..13607
                     /locus_tag="KPE55_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0491319.1"
                     /translation="MDYKVDDKNLDASMFISFVNQVWQGDYDMEQTRQALSKTLNITA
                     YDNKKLVGCLRILSDGYYFGTITELLVLPQYQKQGIGSKLLQLAKDNTPTMLYFGSQP
                     EAEKFYEKNGCQKSLQSYMINKG"
     gene            13714..14010
                     /locus_tag="KPE55_000215"
     CDS             13714..14010
                     /locus_tag="KPE55_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0491320.1"
                     /translation="MNDIFKNMQAKIGCEYLSDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSYQTLKDVMKQQIGREEQCRKQGYWWKRKEQLTKKQHHTGSTCR"
     gene            13929..14432
                     /locus_tag="KPE55_000216"
     CDS             13929..14432
                     /locus_tag="KPE55_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0491321.1"
                     /translation="MQETRVLVETKRTTDEETTSYWFDLPIEVAEFEEKLGVGAESGE
                     YRIIEKVLPYADEVHEHTSVYQLNKLDFMYRQLSSDMQEEYVSLLMVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFNFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            14450..14953
                     /locus_tag="KPE55_000217"
     CDS             14450..14953
                     /locus_tag="KPE55_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0491322.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFSFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            15074..15472
                     /locus_tag="KPE55_000218"
     CDS             15074..15472
                     /locus_tag="KPE55_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0491323.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            15450..17900
                     /locus_tag="KPE55_000219"
     CDS             15450..17900
                     /locus_tag="KPE55_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491324.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPEKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            17900..20095
                     /locus_tag="KPE55_000220"
     CDS             17900..20095
                     /locus_tag="KPE55_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0491325.1"
                     /translation="MKERIQSAFTKKKMFRFLKITVFVVALSLILLSLLETVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SVSPEDEDGKTREEIVKTEIEDNDNNILTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNASDSQSMGRRIFRRP
                     YLFIRHRARRMERRMARAVSAGGISGGVAGALAGSTVSNARTTRNNPTKENRGNTTSI
                     MGQRAGSKIGAVLDTKNKVKDKANAVKENVKDMPTQTAYAVYSAKEKAKSSVSDFKRG
                     MVQEQQSRQTGRLEKQEQRKKNIANKRMELQKAQEARQARKADGSATTGATRPHERPA
                     TASKPSVEKVQEVKRPATTPTSKASEPVKTNVIKERPLSSGASDGKATQSAQPIHRQN
                     IEKVVSQETRQNYVKDRRTKVQQTQSVQKNQQTTEKNHNLVTKKGQKKK"
     gene            20092..21096
                     /locus_tag="KPE55_000221"
     CDS             20092..21096
                     /locus_tag="KPE55_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG0491326.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLISADSDDENSNFSSGITG
                     LNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSYKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFTLA
                     ESFACEKSGGKKVTYTNTIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLNSSYWQQHLIGAGRVK
                     "
     gene            21116..22018
                     /locus_tag="KPE55_000222"
     CDS             21116..22018
                     /locus_tag="KPE55_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0491327.1"
                     /translation="MFKKSKKQTENVKEKKVRTVKVGTHKKTVIAFWVVLIVSVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQDLNVDTIRTDIPTSSTVTDVIVWNIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKAIYTVKVHVDVDGDMVIIQNPTLAPTIEKSDYELKTPEADASVDADTINDATSFLE
                     TFFKLYPIATEKELAYYVSGNVLEPIGKDYLYSELINPVFTKDGDNVKVKVAVKFLDN
                     QTKATQVSQYELVLHKDSNWKIVK"
     gene            22162..22773
                     /locus_tag="KPE55_000223"
     CDS             22162..22773
                     /locus_tag="KPE55_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491328.1"
                     /translation="MPKGIMLTQEQQEERREEIISVALQLIEKNGFQKTSMRKIAILA
                     NMGKSSLYDFFKTKDEIFVYAVEKEIEETIKKVHRIIADESSPEQCLRKIMLNHLGVP
                     KQYRTVLMWLNTESDYLEEDYRKRLKTARYAYQDIIKSVIENGMESGVFRKTNANLMT
                     RLLINSIISIIYTSRPSASPEKMLDETMDIFLHGIMNDGGELL"
     gene            22770..25337
                     /locus_tag="KPE55_000224"
     CDS             22770..25337
                     /locus_tag="KPE55_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0491329.1"
                     /translation="MIYYTLPLLHKQATLEMVGGKGMSLSKLLTTGIPVPEGFHVTTT
                     SYKIFVEKNHIQPYINKLLDGIDTNNTSQLENVSTQIGMLFHNGEMPQEVSDAIKTAY
                     ARLGNIAVAVRSSATAEDLPDASFAGQQETYLNIQGENEVLDAVKRCWASLWTARAIA
                     YRVKNDIKQEIVALAVVVQKLAFSDASGVMFTLNPINGRRSEMIVNAAWGLGESVVSS
                     LVTPDTIVVDKNAERIVSYEVANKEIMTVRNSDGTEEIPTPEQLRKKHALTRNQVMRL
                     TQLGKKIEKYYEMPMDVEWALEKDKLYIVQARPITVLPPEWTLPEKDVIYTRGSLAEH
                     LPNPVTPLFATLGLEIVNRASALLWIDMFGKSAKKLLPENGAYTIINGYVYLSANSKP
                     LLIAVKSLSPRSLRRALTNSVARWETARKEFEDVIKQWEEKPMHMLNAHQIMEGIQTV
                     FYGACIYFTRIQLTLPAASISETLFTKFFQGAARRAGMTDTSVFLLGFDTIALQAEKN
                     LWSLSEWVKQNNTLNLYLQSNPTAKIAENFMSSVPPDEVSLEVWIEWKNRINQYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESIKTFVEGKGESPFLRQSKFEKHRKQAEEEILQHI
                     GSSRKKLFLKLLHWAQETAPMRENAIYCMGMGHPLIRQMFHEISARLIRGGATSHIDD
                     IYWLTKLELEKLIVQLDKNIPLSDRRNSILARKAELKKYQGYMPPAQLPEKKVKSISH
                     APQKQKDGKTVLRGIGTSSGVVTARACVLNSPADFKNFQPGSVLVAVTTTPAWTPLFA
                     SASGIVTDIGGPLSHSSIVARECGIPAVMATHTATRSIKNGQMITVDGSEGTVTIDE"
     gene            25330..26490
                     /locus_tag="KPE55_000225"
     CDS             25330..26490
                     /locus_tag="KPE55_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0491330.1"
                     /translation="MNKKPIFALNLATLVIALGYSLILPVMPFYMENLGAGGRELGWL
                     TAVYALAQTVCAPFWGALSDRIGRKPIISIGIIGYSISLFLFGLASSFWTLFIARSLS
                     GILSSATSVASLAYVGDSSSEEERSKNMSQLGASMSVGVMMGPLLGGILAGYSLALPF
                     FTGAGISFIAFLLILTLLPESIERSGQTEKRKPFDFSDLKSIIFGSAGMVLILIFVVH
                     FCQTGLQGITGLYVVDKFGFSTKQVGVIWMVLAGILIVVQGFLTGPLAKKIGEKRLIL
                     IGMFCKALVAFAMVLTTGFVSVLVVFGLFAVSSAITVPTLNATLSKTDNQHKGTLMGF
                     ASTAGNLSKVIAPLLTGYLYESNIELPYITTGGIALIGVVICYIWIKNQKNK"
     gene            complement(26677..26870)
                     /locus_tag="KPE55_000226"
                     /pseudo
     CDS             complement(26677..26870)
                     /locus_tag="KPE55_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            26973..27479
                     /locus_tag="KPE55_000227"
     CDS             26973..27479
                     /locus_tag="KPE55_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0491331.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSVLDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            27484..27759
                     /locus_tag="KPE55_000228"
     CDS             27484..27759
                     /locus_tag="KPE55_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HBG0491332.1"
                     /translation="MLITCCFGKDFNLSKFAFEIEHEITPMEQMKAINFSLEKDDCTS
                     KVLIYNPDKWKYVEYYFYKDRPIKKLLEKFIQFCICHNKICFEILNT"
     gene            28029..28601
                     /locus_tag="KPE55_000229"
     CDS             28029..28601
                     /locus_tag="KPE55_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0491333.1"
                     /translation="MNVDTIRTDIPTSSAVTDVIVWNIEQSGTDTLSATYEVDQQIKE
                     REQTTAVKATYTVKVHVDADGDMVIVQNLTLAPAIEKSDYEPKTSETDASVDADTVND
                     ATAFLETFFEVYPTATEKELAYYVSENVIEPIGRDYLYSELVNPVFTKDGDNVKVKVA
                     VKFLDNQTKATQVSQYDLVLHKDSNWKILG"
     gene            28704..29519
                     /locus_tag="KPE55_000230"
     CDS             28704..29519
                     /locus_tag="KPE55_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0491334.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSVFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFFN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            29600..30076
                     /locus_tag="KPE55_000231"
     CDS             29600..30076
                     /locus_tag="KPE55_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0491335.1"
                     /translation="MITYKVNSIGTIYNNEKGAFIQLEAAYIPALRALDGFSHINVLW
                     WFSDFDNTDFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSVVEIINIDYMNGMI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPNVPDWCSHWPHSLEESASFAWENEFNF"
     gene            30090..30641
                     /locus_tag="KPE55_000232"
     CDS             30090..30641
                     /locus_tag="KPE55_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0491336.1"
                     /translation="MSLQDYIIEKPVIDTEHLILRTMDKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPEVLFSKPEKATKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKQYQGNGLMSEALKSVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITS"
     gene            complement(31031..32200)
                     /locus_tag="KPE55_000233"
     CDS             complement(31031..32200)
                     /locus_tag="KPE55_000233"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBG0491337.1"
                     /translation="MFYIGIDVGKFNHCACVISSEGEVLIEPFFFTNNLDGFDSFIKA
                     VKKFKSPRHIVGLEATGHYGDNLINFLITHDYEVALINPLTTDAKRKSKIRKTKNDKK
                     DTFLICSVLFDRDYTRVTQRKLDMKQARELTRYHASIAEDINQKKNQLQKCIDIVIPE
                     FNTWFKTKYSKAYMAVLKEFGSSYNLASANLAHLKKVLKPKSSGSRVNIDASSLRSSA
                     KKSIGENNEAVTLEIKMLIENIELLASQLKTVDKKIEELAVKLDSPIFSIPGIGIYTG
                     MSILSEIGDIHNFSSAVKVMGFAGVDPGTYQSGEYSAPRTALSKRGSRYLRKSLYQCI
                     LTVCKYNLTFNKYYKLKRLQGKSHRCAQGHSVRKLIRVIYKLLSENIQFDEKLLI"
     gene            32479..33390
                     /locus_tag="KPE55_000234"
     CDS             32479..33390
                     /locus_tag="KPE55_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0491338.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKK
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEVWIIPV"
     gene            33468..33902
                     /locus_tag="KPE55_000235"
     CDS             33468..33902
                     /locus_tag="KPE55_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0491339.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            34370..35110
                     /locus_tag="KPE55_000236"
                     /pseudo
     CDS             34370..35110
                     /locus_tag="KPE55_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            35061..35672
                     /locus_tag="KPE55_000237"
                     /pseudo
     CDS             35061..35672
                     /locus_tag="KPE55_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            35885..36742
                     /locus_tag="KPE55_000238"
     CDS             35885..36742
                     /locus_tag="KPE55_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0491340.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            36820..37401
                     /locus_tag="KPE55_000239"
     CDS             36820..37401
                     /locus_tag="KPE55_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491341.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            37505..38350
                     /locus_tag="KPE55_000240"
     CDS             37505..38350
                     /locus_tag="KPE55_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0491342.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            38347..39030
                     /locus_tag="KPE55_000241"
     CDS             38347..39030
                     /locus_tag="KPE55_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0491343.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTV
                     IALILGVVSDSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            39027..39725
                     /locus_tag="KPE55_000242"
     CDS             39027..39725
                     /locus_tag="KPE55_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0491344.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            39737..40648
                     /locus_tag="KPE55_000243"
     CDS             39737..40648
                     /locus_tag="KPE55_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0491345.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFTAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECAASIILSVALAITGAG
                     IGYSGIKKIFSEQYNTLSTPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            40700..40840
                     /locus_tag="KPE55_000244"
     CDS             40700..40840
                     /locus_tag="KPE55_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491346.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNSPKDSEDDK
                     MY"
     gene            41130..41756
                     /locus_tag="KPE55_000245"
     CDS             41130..41756
                     /locus_tag="KPE55_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491347.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKVNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            41878..42594
                     /locus_tag="KPE55_000246"
     CDS             41878..42594
                     /locus_tag="KPE55_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0491348.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            42758..42949
                     /locus_tag="KPE55_000247"
     CDS             42758..42949
                     /locus_tag="KPE55_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG0491349.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            42973..43203
                     /locus_tag="KPE55_000248"
     CDS             42973..43203
                     /locus_tag="KPE55_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491350.1"
                     /translation="MIGLKRRNMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            43470..43889
                     /locus_tag="KPE55_000249"
     CDS             43470..43889
                     /locus_tag="KPE55_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0491351.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            43892..44146
                     /locus_tag="KPE55_000250"
     CDS             43892..44146
                     /locus_tag="KPE55_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0491352.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLVFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            44518..44721
                     /locus_tag="KPE55_000251"
     CDS             44518..44721
                     /locus_tag="KPE55_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0491353.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRRLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            44799..45989
                     /locus_tag="KPE55_000252"
     CDS             44799..45989
                     /locus_tag="KPE55_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0491354.1"
                     /translation="MSKVKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVININHQLLRDTEVGYYISTPKTKNGKRKLPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            46229..47050
                     /locus_tag="KPE55_000253"
     CDS             46229..47050
                     /locus_tag="KPE55_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG0491355.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            47071..48267
                     /locus_tag="KPE55_000254"
     CDS             47071..48267
                     /locus_tag="KPE55_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0491356.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            48435..51833
                     /locus_tag="KPE55_000255"
     CDS             48435..51833
                     /locus_tag="KPE55_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0491357.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            51843..53516
                     /locus_tag="KPE55_000256"
     CDS             51843..53516
                     /locus_tag="KPE55_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0491358.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            53789..53983
                     /locus_tag="KPE55_000257"
     CDS             53789..53983
                     /locus_tag="KPE55_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG0491359.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    54152..54385
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            54462..55859
                     /gene="asnS"
                     /locus_tag="KPE55_000258"
     CDS             54462..55859
                     /gene="asnS"
                     /locus_tag="KPE55_000258"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG0491360.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(56032..57207)
                     /locus_tag="KPE55_000259"
     CDS             complement(56032..57207)
                     /locus_tag="KPE55_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0491361.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
BASE COUNT        19862 a         9118 c        11802 g        16555 t
ORIGIN      
        1 aaaagtggtg cctttgatta aactaagttt ttataattta aattttgtac aataagctat
       61 cttttattat tctttaaaat atatatttca tttaattatg acaaataaag ataaaaatta
      121 aatattttag aggtgatttt atttagatat agaattttag aataatacta tacaaatgta
      181 ttgtattatt atttttatat caaaaacaca ctaaaaattt gattatccgc taaaacgctg
      241 tatctatcgg cattatcaag gagctaaaca catcaaaata ctactaattt actacgattg
      301 aatgagcctt gatacgtcct atttcccaga tatgcaaaga tacggtacag cacatcagta
      361 ttgaaacaag aaaaattgaa tacttcatag actacggaac gcacaatatc agcgttcctt
      421 ttctatttcc agccgttcct agtggacggc tattttacta ccaaaatgaa aggagcattt
      481 agaattatga aaaagatatt aaaacgattg tgtacaggct ttcttgcctt tgcaactgtc
      541 gttaccgctt taccgagttc aacggttcat gcgtctaata cacagtattg gacggaaagt
      601 aaagagcgtg tcggtatcgt agaacaagta atgaatgacg gttctatcag ttctaccttt
      661 aatgagggac atttaaccgt tgagggcaag gacgcttatt gtattgacat caacacagcg
      721 tttaaaaacg gctataagac cagaacagac gcaagctcac gcatgagtgc cgaccggata
      781 agcgacgttg ccttatctat tgagtatgtg aaacagtaca cagacagcca cagcggaata
      841 agcagtaaac acgcctacct attaaaacag cttgttgtct ggcagagatt aagtgttcag
      901 cttgactggc aatgcgataa tgtgcgagct tcctatgatg aaatcccaaa agccgtacaa
      961 gatgaagtat tcgcaggagc aaaagccttt gtgaaagaaa acaaaggacg ttatgactgt
     1021 ggcggatata tctattctgg cgagggtcaa gagttagggc aattctgggc gaagttagct
     1081 gtcggaaatg ccaaacttca aaagacttcc accaatgcca atattacaga cggcaacggg
     1141 atttattcaa tcgctggtgc aacatacggt gtgtattctg ataaagactg cacgaaacag
     1201 cttgccaccc ttacgactga taccagcgga aatacagaag ccgttgaagt aagagccact
     1261 acagtatata tcaaggaact ttctgcacca gcaggattta aaattgataa aacagtttac
     1321 tctttatctg tggaagctgg aaaaacagcg actttaaaag tatctgatac accaaaagta
     1381 acagatacct tgattgagct attcaagatt gacatggaaa cacagaaatc caattcgcag
     1441 ggaaacgctt ctttagaggg agcagagttt acatggaact tctacgctgg atattacaac
     1501 aagaacaatc ttccagcaca gcctacaaga acatgggtaa caaagactat cgctgaaaaa
     1561 gacagcgacg gagctatcca ttacattacc cgtttagcta acgcttataa agtatctgga
     1621 gacagcttct atatgcagga cggtaaaaac gtgcttcctt taggaacact aaccgtcgaa
     1681 gaaacaaaaa gtccgtctgg ttacttatta gagggtgcat atatgcaggc agacggtagc
     1741 gaagaacaga taaaagaaat gtatctgaca cagattacag aggacggcga ccttgccgta
     1801 ttgtctggaa gcaatcaata ccatgtttct gataaagtca ttcgtggcgg tgtaaaaatc
     1861 cagaaacgag atttagaaac caaagacacg aaagctcaag gcggtgcgac tttgaaaaat
     1921 accgcctttg aaatcatttc cttaaatgag aatgccgtct tagttgagag caagttatac
     1981 aagaaaaatg aagtgttaaa gacaatccat accgacattg agggtatcgc ttctacttct
     2041 gctgacctat taccttacgg aaaatacaga ttgtctgaac aaaaaccgcc agagggatat
     2101 ttgactgacg gtgcaaagcc gattgatttt gaaatcacag agaatggaaa aatcgttgat
     2161 ttgactgacg aagcccactc tatctataac cagattaaac gtggcgatat tgagggcgta
     2221 aaaattggtg caggttcgca caagcgtctt gcagatgttc cgtttaggat cacaagcaag
     2281 acgacgggcg aaagtcatgt tgttgtaact gatgataacg gacaattctc tactgcttct
     2341 gattgggctt cccacaagca caataccaat gcaggaaaaa caagcgagga cggtgtgtgg
     2401 tttggtactt ctgaaccaga cgacagcaaa ggtgctttga tttatgatac ctacatcatt
     2461 gaagaattac gttgtgaaag caacaaaggc ttcaaactta ttccaccgtt tgagattgtc
     2521 atttccagaa ataaggttgt ggttgattta ggtacgctga ctgatgaata cgaaaaagaa
     2581 atcactatcc atactactgc taccagcaag gacggcgaaa agactatcct tgcagggaat
     2641 gacgttacta tcattgatac ggtcaaatta gacggtcttg taaaaggtac aaagtatcag
     2701 ttaaaaggct ggcagatgtt gaaaaaagaa aatgccgagc ttcttgtagg tgataaacga
     2761 gtggaaagtg attatacctt tgtcgctgat gatgaagcta tgaaagtgga aatcgcttat
     2821 acattcaatg cgtctgctct tggcggtaag aaccttgtaa cttttgaaga attgtatgat
     2881 ttaagcaatc cagaagaacc cgtgaaagtt gcggaacata aagatattga ggacgacgga
     2941 caaacggtac ttatcacaga acgtatcatc aaaatccata ccacagctac ggacaaggac
     3001 ggcaagaaag agattgaagc tggcaaggac gtaacgattg ttgatacggt caagttagag
     3061 ggcttagaag tcggtacaaa gtatcagctt gtgggctggc agatgttaaa agaagaaaat
     3121 gcagagctta tcatcaatga taaaaaagtt gaaaatgact acacatttac gtctgacagt
     3181 gaaactatgg aagtaaagat tgcatttact tttgacgctt cttctcttgg cggtaaacag
     3241 tttgtaactt ttgaggaatt atacgattta agcaatccag acaaaccgat aaaagttacg
     3301 gagcataagg acattgagga cgacgggcag acggttacta ttaaggaagt accagaaaca
     3361 cctacgccag aagaaccaga gaagccaact acaccagata caccaacgaa aacagacagt
     3421 ccaaaaacgg gcgacaatac aaatattctt gcctttgcga ttatgatgtt tgtttctgct
     3481 ggcggtctgg ctggtacata cttcttcaaa cgccgtaaga tgaagaaatc ataaggcggt
     3541 aactgcatga gaaaagaaaa atcacgctct ccgccgtaac ttaaataggc agactacttc
     3601 tattctggct tgtctgccga ttacctgcaa gaacccagat cgtcaagagt gcgtcagcat
     3661 acagtttacc ctttactgtc tgggcttgct ggtatctcat taactgccag aatacaaaac
     3721 aatcaaacta atagaaagaa acgaggtaaa acaatatgga attaaaatat atcgtgccga
     3781 atgtggcaca gacttttgga aacttggaat ttgcagggga aaacaaagta gaccagcaac
     3841 atatcaatgg gcgttacact cccgtatcaa gaagttacaa cttgtattcg gatattcaga
     3901 gagccgacga cattatcgtt gtattgcctg caaaagctgg cgagaagcat tttaagccag
     3961 agcagaaagt aaaactgatt aacccacgca ttaccgcaga gggttacaac gtcaaaggac
     4021 gtggatttac aaactacatt ttaatggctg atgatatgtt accagcagaa agcaaataag
     4081 ggggtatttg attatgagat tagcaaatgg aattgtatta gataacgaaa aaacttttgg
     4141 agtattgaaa ttctctgcac ttcgacatga ggtgcgtgta gaaaatgagg acggaacgac
     4201 aagtgatgaa attaagcgtc gtacttatga tttgaaatgt agtgtgcagg aacgcatgat
     4261 acaagtgtca atccccgctg atgttcccgt aaaggacttt ccttataatg cggaagtgga
     4321 gttaatcaat cccgtagctg atacggtggc aaatgccaac tttagagggg ctgatgtgga
     4381 ctggtacatc aaggctgacg atattatctt gaagaacaaa ggcaaccagc cagcaggaaa
     4441 tccacagaac aacaatccac agaaccttgc accacaagga caacaaaaaa ataaactggc
     4501 aaactagatt ttcattgtag caaatataag tgactgttat aatatgggta tggaaacaga
     4561 taatttgaac ttgcggaggt agcgaaaatg aaatttaaaa atcctatgtt ggtagtgact
     4621 gatatagata aatcagttga gttttataaa aaagtttttg gacttcgtgt aattatggat
     4681 tttggagcaa ataaaacttt aacaggtggc ttagccttac agacaagtga aacatacaaa
     4741 gagtttatcg gtacaagtga tatttctttt ggcggtaaaa attttgaagt ctattttgaa
     4801 gaagatgatt ttgataaatt tgctgatagg ctgaaagaat atgatattga atatgtccac
     4861 cctattatag aacattcatg gggacaacgt gtagtccgct tttatgaccc agataaacat
     4921 attatcgaag ttggagaaaa catgaaaata gtatgcaagc gtttcttaaa tagcggaatg
     4981 acaccagaac aagttgcaga acgcatggac gtgcctatga aatttataaa tgcctgtatg
     5041 agataaattc ttgtttgtag aagtcaataa atacaaagat aaataagaag ttataaagga
     5101 gaaatgagta tgctggataa aatacctaat gcagaagaaa tgactgcttt agtcgggcaa
     5161 tcattatatg ctatatggaa taaactgtgt gccttaattg atgaaaagta tgacatggaa
     5221 tgtttatgga acaaaggtgg taaagcgtgg acttatgagt ataaatatcg tcgtggcgga
     5281 aaaacgcttt gtgcattgta cgcaagggaa aactgtgtag gatttatgat tatcttaggg
     5341 aaagatgaac gattaaaatt tgatacagat agaaatagtt attcaaaaga agtgcaaaaa
     5401 atttatgatg aaacaaaaac ctatcatgac ggaaaatggt tgatgtttga gccaacagat
     5461 acaatcttat ttaatgattt tgttaaatta ttgggaataa aaagaaagcc caacaggaaa
     5521 tgatgatttt atacttcaat ttagtataca aatcggaagt tgaggaggac aactattatg
     5581 gataaagtag ttatttatat acatggtaaa ggtggaaatg cagaggagtc ttcacattac
     5641 aaacctcttt ttagtgattg tgatgtggtt ggactcaact atactgcaca atatccatgg
     5701 gaagcaaaag aagaatttcc atcacttttt gatttgattt gcaaacatta caaatctgtt
     5761 caaatagttg caaatagcat aggtgcctat ttcgccatga acgccttatc agataagcaa
     5821 atagaaaaag catattttat ttcacctatt gtgaatatgg aaaaactgat tgcagatatg
     5881 atgtattggg caaatgttac agaagatgaa cttcgagata aaaaagaaat taaaacaact
     5941 tttggagaaa cactctcatg ggaatatctc tgttatgtaa aagaaaatcc aactacatgg
     6001 aaaattccaa caaatatttt atttggtgag aaagataact tgacttctta tgcaactata
     6061 tctgaattta caaaacaaac taatgcaaca cttactgtta tgagaaacgg agagcattgg
     6121 tttcacacgg aagaacaaat gaaatttctt gatgagtgga taaacaagtc tgccagataa
     6181 atcccagttt gtaaaagtca ataaatacaa agataaattt gaagttagga gactggtact
     6241 atgaaaggat ttgaacgaaa aaaccaatta ttttctcttt gcggattgaa ttgcggacta
     6301 tgccctatgc ttttgggaaa ttactgtggc ggttgtggca atggtaatca atcatgcaga
     6361 attgcaaaat gtagtcttga acatggacag attgaatatt gctatgaatg caaacaatat
     6421 ccatgcgaaa aatatcaaca cattgatgat tatgattctt tcatcacgca taaacagcga
     6481 aaagcagatt tagaaagagc aaaaaacata ggcattgagc aatataatct tgaacagcag
     6541 gaaaaaacac aaattctttc ttacttactt tctaactata atgacgggcg tagaaagact
     6601 ttcttttgtg tggcagtcaa tttattagaa ctttcagaat tacaagaagc aatgaaacaa
     6661 atacaatcca atgacgaatt gaccttacta ccatttaagg cacaatgctt gtatgtggtg
     6721 gaggtgtttc agaagattgc tgaaaggaga aatataaaac tgaaactttt caagaaaaag
     6781 tgattttcaa atttgaaatt gtagcaaaga gttgtaactg cattgggaag ctatttatat
     6841 atcctaatgc ctatactgaa attcaaacaa actgcaaaat atcatgagga atatacgctg
     6901 gtattttcta aggaaaacat taaatttaaa acacaaagca ttgactcaga agtgaagtgg
     6961 gacatttact ctgcactatg ggaaagccat gatttttatt acttaattca agcaccacga
     7021 ttatatacgt taattcccaa aagagttttt aaagacctaa acgaaaaaca gctatttgaa
     7081 gaaattgcac aatcaagagt taaaacaaca aagcatatat aatgaacata ccctacaatg
     7141 taacaacttc atattcatac acacaaagcg gttagtcttt taagattgac tgcttttttc
     7201 atacttaaat ttaagattgg agtgattgat ttatgcgaat aatttggaat aaaggacacc
     7261 gtatcagagc cagcgataag aacctcgtct atcgcttttc attgggaacg cttctaattg
     7321 tattcgtggt ggttcttctg ctactgaata tgaaacagct catgcgtacc gactgggaac
     7381 atttcagctt attagacaac agctttacgc tttccttata caacttcata accttactga
     7441 tagcgactgg tgtttgtgca ttggtcgctt ttctgtatta ccgcttttgt tatgacaatt
     7501 ttaaaaagct actgcaccgt caaaagctgg cacgaatgat actggaaaat aagtggtatg
     7561 aagccgatac cgtacaagac agcggttttt ttactgacct gcaaagcaga tcaagggaaa
     7621 aaatcgtctg gtttccaaag atttattatc aaatggaaaa gggactgctt catatccgct
     7681 gtgaaatcac attaggaaaa tatcaagacc aacttttacg gttagaggat aaattggaaa
     7741 gtggcttgta ttgcgagctg accgacaaga ccttacatga cggctatatt aaatatatcc
     7801 tgctttatga catgatagca aaccgcatta ctattgatga agtacgggca gaaaacggct
     7861 gtcttagact gatgaaaaat cttgtctggg aatatgacgc actccctcac gctctgattg
     7921 ctggtgggac tggtggcggt aaaacctatt ttcttctgac gctcattgaa gccctactgc
     7981 ataccaacgc tgtcctttac atcttagacc caaagaacgc tgaccttgca gatttgggaa
     8041 cagtcatggg aaacgtgtat cacactaaag aagaaatgat tgattgcgtc aatgcctttt
     8101 atgagggcat ggtacaacga agcgaagaaa tgaagcaaca cccgaactat aaaacaggag
     8161 aaaactacgc ctatcttagc ttaccgccct gctttttaat ctttgatgaa tatgtggcgt
     8221 tttttgaaat gctggggaca aaggaaagcg tgagtttact tagccagtta aagaaaatcg
     8281 tcatgctggg gagacaagca gggtatttcc ttatcgttgc ctgccagcga ccagacgcaa
     8341 aatatttctc ggacggtatc agagataact tcaatttccg tgtagggttg ggacgtatca
     8401 gcgaattagg ttacggtatg ctgtttggtt ctgatgtgaa aaaacagttt ttccagaagc
     8461 gtatcaaagg gcgtggctat tgtgatgtgg gaacaagcat cataagtgaa ttttacacgc
     8521 ctttagtccc gaaaggatat gattttttgc agaccatagg ttctcttgca tatgcaaggc
     8581 aggacgggac ggcgacgtgc gaagcgaaag gcgacggcac ggactagccg ttgctggtgt
     8641 ggctaacgcc acaccagcag aaacaagccc ctcattatct aacagagggg cacaattacg
     8701 gcggaaactt ccacaaaaac ctttgcaaga ccgcatggaa tgagggtttt tcgtacatct
     8761 cgccttttgt gataaaacct tgaaattatc acaatatcac aaaattatca cgattggggg
     8821 tatgacaact gaatgaaact gattttttaa ccgccttgaa agcgaaacgc tgtgcataca
     8881 gcgtatcgca gacaaggctt gcaatcatgg caaatatcag ccgtgaacat ttgagccgta
     8941 tcgaagcagg aaaggtaacg ctgactgagg acatgaaaca tacgctttta gaagcggtgg
     9001 aaaaattcaa tcccgacaat cccatgttcc tgctgtttga ttatgtgcgt atccgttttc
     9061 ccacaatgga tataaagcat atcatcaaga atatattaaa gctcaatatc gactatatgc
     9121 ttcacgaaga ttacggacat tataaataca ccgagcatta tcatatcgga gatgtgtttg
     9181 tgtatgtatc gcaggacgag gaaaaaggtg tcctgctaga actcaaaggc aaaggctgtc
     9241 gtcagtttga aagttatctg ctggcacaag agcgaaattg gtatgacttc ttcacggacg
     9301 cattggttga gggcggtgtg atgaaacgtc ttgatttggc aatcaatgac cgaacgggtt
     9361 tacttgatat tccagagctg atacaaaaat gcgagaatga ggaatgtatt tctaaattcc
     9421 gttctttcaa aagctatggt tctggggaac tggtaaagca taacgaaacg gataaatacg
     9481 gtatgggaca cacgttgtat atcggttcat tttcaagtga agtatacttc tgctgttatg
     9541 aaaagaatta cgaacagtac gcaaaacttg gcataccgat tgaagaagtc cccataaaaa
     9601 atagatttga gatacgtttg aaaaatgaac gtgcctatta tgctgttgtg gaactgctga
     9661 cacattatga cgcagaacag acagcatttt ctattatcaa ccagtatatc cgctttgctg
     9721 ataaagaacc agacaagcga aaaagcgact ggaaaacaaa tgtacgctgg gcttggttca
     9781 ttggcgaggg gcgaccaccc gtaaagctga caacaaagcc aaaaccgtac acgttagaac
     9841 gtacactcaa ctggctacaa aggcaggttg caccgacctt gaagatgttg aaacagatag
     9901 acaaggacaa tcagacggag tatctggaaa cgattgagaa gcaggcaaaa ctgaccgagc
     9961 gacaccacca gataatcaag caacagacag ccacggttga agaaatggtt gccaaagtaa
    10021 aggagtgatg aaaaatgtgg gaattattga aagacttcct gctggtatct ctgggaatgg
    10081 gtatcggcgt agtcttgatg tgcattttaa ctgtcggcaa agaagctgac agatgtatgg
    10141 aagaaatcaa agaaagagag gaacattaaa tgaattttgg acaaaattta tgtgcgactc
    10201 gttccttagt gaaaagctaa ggcactaata aaagaacgaa aaagtttgat tattaggaga
    10261 actgataatc tgacaggtgg aatgatgaag taacgctctg aaacgcctgc cctgagcctc
    10321 cgacaagcat tgaatgagga aactcagaat gtttgaagct cggtaaagac ggctgaaaga
    10381 taccgtaata gttgatgaac atatatcttc tatcgaaaac tatccagagg tgggtagcgt
    10441 atccaacagg tcgggggtct ataaaatatc tatggttaga atgttccaca agggactgac
    10501 gaaacagcga atgtacgggt ctaaatttgt actttgtaga aatgcaaagg tacgagaaat
    10561 cgtagctggt gtggcaaagt aagaatatag gttatgaaat gccatatacc gttacaggcg
    10621 gtatcaagcc agcaggctta tagctagtga cctaaggata tatatgtata gataagatta
    10681 tcggaacgag gaaaggtatt gggttctcat caagagaata agctgacgaa gaacaataag
    10741 cagttgaacc aatgctgaaa agcagagggc gaacctaaga aattcttgta atgagaatgg
    10801 aggaataccc tcaagtcgtg tatatattag aaaagtatta tgttgtttta ataaggatta
    10861 cgagtaagat aaaaaagctc actcaatttc aagcaaagga gtgaagcctt atgcctaaaa
    10921 ataaaaatga aacattgtgt gtagaagatt taagacacgc tgaatattac gaaatgcaaa
    10981 acacctttga tgatttatat gccaaaagta agaatggaga tattttcact catcttatgg
    11041 atattatcct atcgagagaa aatatattac tggcatatcg aaatatcaaa gcaaatgcag
    11101 gcagtaagac agcaggaaca gacggtacaa taattaaaga tattggcaaa ttgccagcag
    11161 aaacagttgt caaaaaggta agatatattg tcgcaggcac tccacacggg tatcgaccta
    11221 agccagtaag gcgaaaagaa ataccaaaac caaatgggaa aaccagacct ctaggcattc
    11281 cttgtatgtg ggacaggctt atacaacaat gtatcaaaca agtattagaa ccaatctgcg
    11341 aagctaaatt tagtgaaaac agctacggtt tcagacctaa taggtcggtt gaaaatgcaa
    11401 taaaagcaac ctataaccgc ctacaaatca gccagctaca ttatgtgatt gaatttgata
    11461 ttaagggttt ctttgacaac gtgaaccatt ccaaactgat aaaacagata tgggctatgg
    11521 gaatacggga caagcacttg atattcattt taaaaaggat attaaaagct ccaattaaaa
    11581 tgcctgacgg cacaataaca tatccagaga aaggaactcc acagggggga attatatccc
    11641 cactactagc caacattgtt cttaatgagt tagaccattg ggtagaaagt caatggcatg
    11701 aaaatcctgt tacaaaaaac tatgtggttc atatcaataa aagcggtagc ccatgtaaaa
    11761 gcaacgctta caaagaaatg aagaaaacaa agttaaagga aatgtatatg gttcgttatg
    11821 cagatgattt tagagttttc tgtcgctaca aagaaagtgc tgaaaaaacc aaaatagcaa
    11881 ttactcaatg gatcgagcaa cgactaaaat tggaagtatc acaagaaaaa acaagaattg
    11941 taaatgttag aaaaagatac tccgactttc ttggtttcaa gattaaaatg ataccgagaa
    12001 gaaagaagtt agttgttaaa tcacatataa gtgataaaca attcaagacc caaaaagaca
    12061 agctggtatc acaggcaaag aaaattgctt ctccctcaaa gggaaaaaca gaattagagg
    12121 aaataagact ttataattca atggtattag gtatgcaaaa ttattatcag attgcaacaa
    12181 atgtaaatct ggattgtatg aaacttaata gagctgtaat ggctatcttc actaacagat
    12241 taggtgcaag caaaaagggc agattgagaa aaacaggcag acaacttaca acaaaagaag
    12301 caacaagata tggtaaatct aaaatgcttc gatatgttgc tggcgagggc gaacctatat
    12361 tccctgttgg atatatccaa cacagaaaac caatggcaaa agtgtattca gcaaactgct
    12421 atactccaga gggaagaaaa tttatccata ccaatctaag tgttgataaa ttactgatgt
    12481 atcagttgat gaaattatct ttggaaaatc gcacaataga atacgcagac aatcgaattt
    12541 cgctattttc tgcccaacat ggaaaatgtg ctataactct ggaagaattt caacaagcta
    12601 atgaaataca ctgtcatcat attgtaccaa ctagggctgg tggtaatgac gattataaaa
    12661 atcttatttt ggtaaaagag gcggtacatc gtctaataca cgcaaaaaca gaagaaacaa
    12721 tacaaaaata tatagctcta ttgaaactaa ataaagtcca gctcataaga ctaaataaat
    12781 atagagtatt agctggcaat caagaattaa acttgatata ggtctactac ataaatactt
    12841 ttcaatataa acacgatgga acgcggagtg cggtgaaagt cgcacgctcc gtgtgaatgg
    12901 ggggaaaagt ttgagatatt tataatatca ggaacttacc tatccatata cacttggttt
    12961 ttaagcaacg cacagagttt agtgcttatg gcgattgtgg taattggtat ctacttaggt
    13021 tttaagcgtg agttttcaaa acttatcggc ttcttggtag ttgctcttgt tgccgttggc
    13081 ttggtattca acgctggtgg tgtgaaagac gtattgttag aactgttcaa taagattatc
    13141 ggtgcataaa attttattgt agtgtgagtg tagaaatggt atgatatgga tatgaagaaa
    13201 gcaacaaatt agaagttgga ggaacaataa tggactacaa agtagatgat aaaaaccttg
    13261 atgcatcaat gtttatttct tttgtcaatc aagtatggca gggcgattat gatatggaac
    13321 aaacaaggca agcattatct aaaacactaa acataacagc ctatgacaat aaaaagttag
    13381 tagggtgttt gcgtattctt tccgatggtt actattttgg aacaattaca gagctacttg
    13441 ttcttcctca atatcaaaaa cagggtattg gaagtaaact tcttcaactt gcaaaagaca
    13501 atacacctac aatgttatat ttcggttctc aaccagaagc agagaagttt tatgaaaaga
    13561 acggctgtca aaaaagctta caatcttaca tgataaataa gggataataa gagataactt
    13621 ccagcttgac aattttatat ccatatacac aaagcagtta gtcttaggat tgactgcttt
    13681 tttcttacct aaaaattaga ttggagtgat gtaatgaacg atatttttaa gaatatgcag
    13741 gcaaaaatcg gctgtgaata tctttctgac ctgccctctt acaaacgtaa ggtgtggcat
    13801 gaaatgaaac aactgaacct tgccgattat gaagagaaac agttagaaga tttttcaaag
    13861 tatgtgtttg gtatgtcata ccagacctta aaagatgtga tgaaacaaca gataggacgt
    13921 gaggaacaat gcaggaaaca agggtactgg tggaaacgaa aagaacaact gacgaagaaa
    13981 caacatcata ctggttcgac ctgccgatag aggttgccga atttgaggaa aaattaggtg
    14041 tcggtgcaga aagtggggaa taccgtatta tcgaaaaggt attgccttat gctgatgaag
    14101 tccacgaaca tacaagcgtg taccagctca acaaattaga ttttatgtac cgccagcttt
    14161 caagcgatat gcaggaagaa tatgtatcac tacttatggt gtttgagaat ttagaagcac
    14221 tttatatttg cagaaatgta attaccgttt atcccgactg taaaagtatg atagatgttg
    14281 caaggcaaaa gctgatgaac gacccgacgt tcaaacattt atctgaggac tgtcaagaat
    14341 actatttcaa ttttgaagcc tacgcttctc acttgcagga acacgggaaa tttttagtaa
    14401 cagaacacgg tatctttgaa ctgccagagt aggaagtgag gtggggctta tgattgatga
    14461 tatggcggtt tatattgcta atcttggcaa atacaatgag ggatatttag tcggtgcgtg
    14521 gttctctttc cctattgacg aggaagatgt gaaagaaaaa atcggtttga atgaacaata
    14581 tgaggaatac gctgtccatg atactgacaa cttccctatc gacatagggg aatatgtttc
    14641 cattgaagaa ctcaatgaga tgtatgaaat gatagaggaa cttcccgact atatcgtaga
    14701 gtgtctggac gaatttatca gccactacgg gacgctggaa gaagtcgtgg aacacaagga
    14761 cgatatttat tattatccag actgtgaaac catgacagac gttgcctact actacataga
    14821 cgaattgcag gcacttggcg acattccacc cagcttacag aactacattg actatgaagc
    14881 ctacggtcga gatttggata tgggcggttg ctttattgaa acaagccgag gtatgtgcga
    14941 gataccttat taacgctgga agatcagcga caagcattgt tgctactgac agcgtatttc
    15001 tcttttaagg gacagtctga aacatggctg tccttttctc tttttaagaa cttacgaaag
    15061 gagctgaaag aacttgaaaa agattaaaag ctacacgggt atctggaacg tggaaaaagt
    15121 cttgtatgca atcaatgact ttaacttacc ctttcccgtt acctttacac agattacatg
    15181 gtttgtaatt acagaattta tcatcattct gtttggggat atgcccccac tttccatgat
    15241 tgagggagca tttctcaaat actttggtat tcccgttgct ctcacttggt ttatgtcgca
    15301 gaaaaccttt gacggaaaga agccgtacag ctttttgaaa tcacagataa cctatgccct
    15361 gcgacctaaa atcacttatg caggaaaagc cgtaaaactg cataagcaga ccttgaatga
    15421 aacaatcacg gcagtaagga gtgtgaacta tgttcccgat aaaatatatt gacaataacc
    15481 ttgtctggaa caaggacaat gaggtgttcg cctactatga gctgatacca tacaattatt
    15541 ctttcctatc cgcagagcag aaatttatcg tgcatgacag ctttcgacag ctaatcgcac
    15601 agtcccgtga gggtaaaatt catgcgttgc agattgccac agaaagctca atacgaagta
    15661 tgcaggagca gtcaaagaaa ctggtaacgg ggaaacttcg tgaggtggca gtccaaaaaa
    15721 tagacgaaca gaccgaagcg ttagtatcta tgattgggga caatcaagtg gactaccgct
    15781 tttttcttgg ctttaagctc atggttacgg aagaacaact caatctgaag aatattaaaa
    15841 aatcggcatg gctgacgttc acggaatttc tccatgaagt gaaccacacg ctgatgaatg
    15901 attttgtttc catgccgaat gatgaaatca accgttacat gaaaatggaa aagttactgg
    15961 aaaataaaat ctcccgtcgc tttaaggtgc gtcgcttgga aatcaatgat tttgggtatc
    16021 tcatggaaca tctttacggc agggacggta tcgcctatga agattatgag taccagctac
    16081 cagagaagaa attgcagaaa gaaacgctga taaaatacta cgaccttatc cgcccgacaa
    16141 ggtgtgtgat tgaggaaagc cagcggtatt tacgattgga acatgaggac aaggaaagct
    16201 atgtgtctta ctttactgtc aatgcgattg taggcgagct tgattttccg tcgtctgaaa
    16261 tcttctattt ccagcaacag cagtttacat tccccgttga tacttctatg aatgtagaaa
    16321 tcgtggagaa cagaaaggct cttagcacag taagaaataa gaaaaaggaa ttgaaagacc
    16381 ttgacaatca cgcttatcaa gcaggaagtg aaaccagctc aaatgtggta gacgctttag
    16441 acagcgtgga cgagctggaa acagacttag accagagcaa agaaagtatg tataagttaa
    16501 gctacgtgat acgggtgtct gcacccgatc ttgacgagct gaaacgccgt tgtgatgaag
    16561 tcaaggactt ttacgacgac ctcaacgtaa agctggtgcg tcctgctggg gatatgctgg
    16621 ggcttcattc tgaattttta cctgccagca agcgatatat caatgactat gtgcagtatg
    16681 taaaatcaga cttcttggca gggcttgggt ttggtgctac ccagcagtta ggggaaaata
    16741 cgggtatcta tatgggctat tccgttgata cgggaagaaa tgtgtacctg caaccgtccc
    16801 ttgccagtca aggtgtaaaa ggtacagtta ccaacgccct tgcgtctgct tttgtcggtt
    16861 cgcttggcgg tggaaagtcg ttctgcaaca atcttcttgt gtattattcg gttctctttg
    16921 ggggacaagc ggttatctta gacccaaaat cagagcgtgg caactggaaa gaaacacttc
    16981 cagaaatcgc acatgaaatc aatatcgtaa atcttaccag cgacaaggac aatgcaggac
    17041 ttcttgaccc attcgtgatt atgaagaatg taaaagacgc tgaaagtctg gcaatcgata
    17101 tcttaacatt ccttactggt atttcctcta gggacggcga aaaattcccc gtacttcgta
    17161 aagcggtacg ctccgttacc cagagcgaca gtcggggctt gctccatgtg atagacgagc
    17221 tacgccgtga agatacgccc atatcaagaa atatcgcaga ccatatcgac agcttcacgg
    17281 actacgactt tgcacacctg ctgttttcag acggtacggt agaaaatgct atcagtctgg
    17341 ataaccagct caatatcatt caagtagccg accttgtgtt gccagataag gacacgacct
    17401 ttgaggaata cacgaccatt gaattgttgt ctgtgtctat gctgattgtg attagtacct
    17461 ttgcccttga ttttatccat tcggacagaa gcatttttaa gattgtagat ttggacgaag
    17521 catgggcgtt cttaaatgtg gcacaaggcg aaaccttatc aaataagctg gttcgtgctg
    17581 gacgagctat gcaggcaggc gtctattttg ttacccagtc tgcctatgat gtttcaaagg
    17641 aaagtctgaa aaataatatc ggcttgaaat ttgcctttag aagtaccgat ataaacgaga
    17701 ttaaacagac cttagaattt ttcggtatcg acaaagagga cgaaaacaac cagaaaaggc
    17761 ttcgtgattt ggagaacgga caatgcttgt tgcaggactt atacgggcgt gtcggcgtag
    17821 tacagataca cccagtcttt gaagaactgc tacacgcctt tgataccaga ccgcccgtac
    17881 agagaaatga ggtggagtga tgaaagaaag gatacagagt gcgttcacaa aaaagaagat
    17941 gttccgcttt ctcaagataa cggtgtttgt cgtggcactt tcccttatcc tgctttcact
    18001 acttgaaacg gtggctcatg caacggggct tgtggacgat acgataaata cagaaaattt
    18061 atattctaaa tatccgttat ccaactatca gcttgatttt tatgtggata atagctgggc
    18121 ttggttgccg tggaactggc tggacggtat cggaaaatcg gtacagtacg ggctttactg
    18181 tattactaac ttcgtttgga ctatcagttt atatttaagc aatgccacgg gctatgtggt
    18241 gcaggaagcc tataagctgg acttcatcaa tgatatggca gacagtatcg gacagagcat
    18301 acagaccctt gcaggcgtta cctcaagcgg tttttcaacc tctggcttct atgtcggttt
    18361 cctgcttctc attatcttgg tggtgggaat ttatgtagca tatacgggac tgattaaaag
    18421 agaaaccagc aaggcacttc acgccgttat caactttgtt gtggtgttta ttttgtcggc
    18481 ttcatttatt gcctatgctc ccgactacat caaaaagata aatgaatttt caagcgacat
    18541 cagtaccgct tctttggact tgggaacaaa aatcatgctc ccaaactctg acagcgaggg
    18601 caaggacagc gtggacttga taagggacag cctattctct attcaagtgg aacagccgtg
    18661 gctactctta caatttggga acagcaacgc agaggaaatc gggacagacc gtgtggaagc
    18721 tctggtatcg gtaagcccag aggacgagga cggaaaaacc agagaggaaa tcgtgaaaac
    18781 agaaatcgag gataacgaca acaacattct gacgataccg caggtagtaa accgtttagg
    18841 tatggtgttt ttcctcttat tcttcaactt aggtatcacg atatttgtat tcttgcttac
    18901 gggcatgatg ttgttcagcc agatactttt tattatcttt gcaatgttcc tgcctattag
    18961 tttcctgctc tctatgatac cgagctatga aagcatggca aagcaggcaa tcgtgagggt
    19021 atttaatacc ataatgacac gggcaggaat aacgctcatt gtaacggtgg cgttcagcat
    19081 ttccagtatg ttttacaata tctccacaga ctacccgttc tttatggtgg cgttcttgca
    19141 gatagtttgt tttgcaggta tctatatgaa gctgggcgat ttaatgagta tgttctcact
    19201 taatgccagc gacagtcaaa gcatgggacg caggatattc cgcagaccgt atctgtttat
    19261 acgacataga gcgagaagaa tggagcgacg tatggcaagg gcggtaagtg ctggcggtat
    19321 ttcgggcggt gtcgctggtg cgctggctgg cagtaccgtt tctaatgcaa ggacaacaag
    19381 aaacaatcct acaaaggaaa atcggggcaa taccacttcc atcatgggac agcgtgcagg
    19441 ctcaaagata ggcgctgtct tagatacgaa aaataaagtg aaagacaagg caaatgctgt
    19501 caaagagaat gttaaggata tgccgacaca gaccgcttat gcggtgtatt ctgcaaaaga
    19561 aaaggcaaag tccagcgtgt ccgacttcaa gcgtggcatg gtgcaggaac agcaatccag
    19621 acagacggga cgcttagaaa agcaggaaca acgtaaaaaa aatatcgcta acaagcgtat
    19681 ggagcttcaa aaggcacaag aagcaaggca ggcacgaaag gctgacggat cagcgacaac
    19741 gggagctacc cgtccccatg agcgaccagc cacagcttca aagccgagtg tggaaaaagt
    19801 gcaggaagtc aaacgccctg ccacaactcc gacttcaaaa gcaagtgagc cagtcaagac
    19861 aaatgttatc aaagagcgtc cgctatcttc gggtgcttct gacggaaaag caacccagtc
    19921 ggcacagcca atacataggc agaatataga aaaagtggta tcgcaggaaa cacgccagaa
    19981 ttacgttaaa gaccgcagga caaaggttca gcagacgcag agcgtccaga agaaccagca
    20041 gaccacagag aaaaaccata accttgtaac gaagaaagga cagaagaaaa aatgaaactg
    20101 aaacatatcg ctcttatcgg cagtctgttt cctatcctct tttccatagt cctttttttt
    20161 ggggtgctga ttagtgcgga cagcgacgac gaaaacagca acttttcttc tggtattacg
    20221 ggtttgaacc tatccgcaga agtcttgaaa catcagccta tggtggaaaa gtacgccaga
    20281 gaatacggca tttccgagta tgttaatgta ttactggcta tcattcaagt agaaagtggc
    20341 ggtacagcgg aagatgtcat gcagtccagc gaaagtctgg gactaccgcc caactcttta
    20401 gatacggaaa gctcaatcaa gcaggggtgt aaatattttg cgtccctgct ttcttcctac
    20461 aaaaatcaag gaataaatga cttgaatgtg gcgatacagt cctataacta tggcggtggc
    20521 tatgtgggat atgtggcagg aaaagggaaa aagcatacgt ttactcttgc agagagcttc
    20581 gcctgtgaaa aatcgggtgg aaagaaagta acctacacca acacgatagc cgttgcgaag
    20641 aatgggggct ggcggtatgg ctatggaaat atgttttatg tggaattggt caatcaatat
    20701 ctgactgtac cgcaggtatc gggagaactg gcacaaaaga taatgaatga agcgttgaag
    20761 tatcaaggtt ggaagtatgt ctatggtggc agtaatccga acacttcctt tgactgttcg
    20821 ggacttgtgc aatggtgtta tggaaaagcg ggtatctctt taccgagaac ggcacaagcc
    20881 cagtatgacg caacccagca tatccccttg tcgcaggcaa aggctggcga tttggtgttt
    20941 ttccattcaa cctataacgc tggttcgtat gtaacccatg tcggtatcta tgtcgggaat
    21001 aatcagatgt accatgcagg cgacccgata ggatatgcag acctcaatag tagctactgg
    21061 caacagcact taatcggtgc aggacgagta aaataaaaga aaggacttag aaaatatgtt
    21121 taagaaaagt aagaaacaga cagaaaatgt caaagaaaaa aaggtgcgta ctgtcaaggt
    21181 aggcacacat aagaaaactg taattgcgtt ttgggtggtg cttatcgtaa gtgtaagttt
    21241 tggggtatat aagaatttta ccgccattga tatgcacacc gtacatgaaa cagaaaccat
    21301 acaattaaga cttaccgata ctaacgggat tgaaaatttc gtgaagaact ttgcgaagtc
    21361 ctattacaca tggaacaaca gcaaagaagc gattgaagca aggacgcagg caatcagcgg
    21421 ttatctgaca aaggaattgc aggacttgaa tgttgatacc attagaaccg atataccgac
    21481 cagttccacg gttacagatg tgattgtttg gaatatcgaa cagtcaggaa cggacacttt
    21541 ttctgctacc tacgaagtag atcagcagat aaaagaggga gaacagacaa cagcagtcaa
    21601 ggcaatctat acggtaaaag tccatgtgga cgttgacggg gatatggtaa tcatacagaa
    21661 ccctacgctt gcaccaacaa tcgaaaaatc ggactatgag ctaaagacac cagaagcaga
    21721 cgcaagtgtg gacgctgata ccataaatga cgctacttca tttttggaaa cattctttaa
    21781 gctgtaccca atagcgacgg aaaaagagct tgcctattat gtatcgggaa atgtgcttga
    21841 acctatcggc aaggactatc tttattctga attgataaac ccagtcttta caaaagacgg
    21901 ggacaatgtg aaagtcaaag ttgccgtgaa attccttgat aatcaaacaa aggcgacgca
    21961 ggtatcgcag tatgaacttg tgctacataa ggatagtaac tggaaaattg tgaaataata
    22021 atttaaagcg tgcatttctt ataagatttg tacgctttac ttttatggta aaaattagcc
    22081 ttgacaagcg accgttggtc gctatataat gaaagcgaaa caagaaacga ccgatggtcg
    22141 ctataaggag gtatcataaa tatgccaaaa ggaattatgc ttacacaaga acaacaagaa
    22201 gaacgccgag aagaaataat cagtgttgct ttgcagctca tagagaaaaa tggattccaa
    22261 aaaacatcaa tgaggaaaat tgcgatttta gcaaacatgg gaaagtccag tctgtatgat
    22321 tttttcaaaa cgaaagacga gatttttgta tatgcagttg agaaagaaat agaagaaaca
    22381 attaaaaagg ttcataggat tattgccgat gaatcctcgc cggaacagtg tcttagaaaa
    22441 atcatgctga atcatcttgg agtaccaaag cagtatcgaa cggtgctgat gtggttaaat
    22501 acagaatctg attatttaga agaagattat cgaaagcggc tgaaaactgc gcgttacgca
    22561 tatcaggata ttataaaatc tgtaattgaa aatggaatgg aatcaggcgt cttccgtaag
    22621 acgaatgcca atcttatgac gcgtttgtta attaactcca ttatatcaat catttacaca
    22681 tcccgaccat cagccagtcc ggagaaaatg cttgatgaaa caatggatat atttctacat
    22741 ggaattatga atgatggagg tgaattatta tgatttatta tactctaccc ttgttgcata
    22801 aacaggcaac ccttgaaatg gtcggcggaa aaggtatgtc tttatcaaaa cttttgacaa
    22861 cgggtattcc tgtgccggag ggctttcatg ttacgactac ttcatacaag atttttgttg
    22921 aaaagaacca tattcagcct tacattaata agttgctgga cggtattgac actaacaata
    22981 cgagccagct tgaaaatgta tctacgcaga tagggatgct tttccataat ggagaaatgc
    23041 cgcaggaagt atcagatgca attaaaaccg catatgccag attgggaaat atagcggtgg
    23101 ctgtccgttc ctccgcaacc gccgaggatt tgcccgatgc ttcttttgca ggccagcagg
    23161 aaacctatct taacatacaa ggtgagaatg aagttcttga cgctgtaaag aggtgctggg
    23221 cttccctatg gacagctcgc gctattgctt accgcgtgaa gaatgatata aagcaggaaa
    23281 tcgtcgcact tgctgttgta gtacaaaagc ttgcgttttc cgatgcgtcc ggcgttatgt
    23341 ttacgctaaa ccctatcaat ggcagacgta gcgaaatgat tgttaacgcc gcgtggggac
    23401 ttggcgaatc ggtggtttct agcttagtca cccctgatac aatcgttgta gataaaaatg
    23461 ctgaacgaat tgtatcgtat gaagtagcaa acaaagagat tatgacagta cgcaactcag
    23521 atggaacaga agaaatccca actcctgaac agttgaggaa aaaacatgct cttactcgca
    23581 atcaagttat gcgattgaca cagcttggaa aaaaaattga gaagtattat gaaatgccta
    23641 tggatgtaga gtgggcactg gaaaaagata agttgtatat tgttcaggct cgccccatta
    23701 ctgtcctacc gccggaatgg acgttaccag aaaaggatgt tatatataca agaggcagtc
    23761 tagcggaaca cttgccaaat cctgttacgc ccttattcgc cacacttggg cttgaaatag
    23821 tcaaccgtgc gtcggcgctt ttatggatag atatgtttgg taaaagtgcg aaaaagctac
    23881 tgccagaaaa cggagcatat acgattatta acggatatgt ttatctttcc gctaattcaa
    23941 agcctctttt gattgctgtc aaatcccttt caccccgatc tttacgccgt gcgctcacga
    24001 acagtgttgc acgctgggaa acagcacgaa aagaatttga ggatgtgatt aaacaatggg
    24061 aagaaaagcc catgcatatg ctgaatgctc atcaaataat ggaggggatt cagactgtat
    24121 tttatggagc gtgtatttat ttcacgagga ttcagcttac attgccagcc gcctctatca
    24181 gcgaaacatt atttacaaaa ttttttcagg gggcggctcg tcgcgctggt atgacagata
    24241 cttctgtttt cctgcttgga tttgatacga ttgctctgca agccgaaaag aacctgtgga
    24301 gtctctcgga atgggtaaag caaaataata ctctcaacct ttatctgcaa agtaatccaa
    24361 cagcaaagat agcggaaaat tttatgtcct cagttccgcc tgacgaagtt tcactggaag
    24421 tatggataga atggaaaaat cgaatcaatc agtattttaa agagtttggt cgtaccgctt
    24481 atgaatttga ttttgcatat tccacaccgc aggaaacact tacaccaacc tttgaatcga
    24541 taaaaacctt tgtagaaggc aaaggtgaaa gtcctttttt acgtcaatcc aaatttgaaa
    24601 agcataggaa acaggcagaa gaagaaattt tacaacatat aggtagctca cgcaaaaaac
    24661 tgtttttgaa gttgctccat tgggcacagg aaactgctcc tatgcgtgaa aatgctattt
    24721 attgcatggg aatggggcat cctctgattc gccagatgtt tcatgaaatt tcagcacgcc
    24781 ttatacgtgg cggagctact tcgcatatag atgatattta ttggctcaca aaattggaat
    24841 tggaaaaact catagtacag ttagacaaaa atataccttt atccgataga agaaattcga
    24901 tacttgcgcg aaaagcggaa ctcaaaaagt atcagggcta tatgccacca gctcagttac
    24961 ctgagaagaa agtaaaaagc atatcacatg caccacagaa acaaaaagat ggaaaaaccg
    25021 tgttgagggg tataggaacc agttctggtg tcgtgacagc tcgtgcctgc gtactaaata
    25081 gtccggcaga ttttaaaaat ttccagccag gaagtgtttt ggttgccgtt actacaactc
    25141 cggcttggac acccttattt gcttcagcaa gtggaattgt aacggatatt ggaggtcctc
    25201 tcagtcattc aagtattgtt gcacgggaat gtggtatccc tgccgttatg gcgacacaca
    25261 cagccacgag aagtattaaa aatggacaaa tgataacggt ggacggctcg gaggggacgg
    25321 tgacgattga tgaataaaaa gccgattttc gctttaaatc ttgctacttt ggtgattgcg
    25381 ttaggatata gcttaattct tccggtcatg ccattttaca tggaaaatct aggtgctggc
    25441 ggtcgtgagc ttggatggct cactgccgta tatgccctgg cacaaacagt atgcgcacct
    25501 ttttggggtg cactgtccga ccgaattggt agaaagccga ttatcagcat tggtattata
    25561 ggatattcca tcagtttgtt cctgttcggt ttggcttcat ccttttggac actatttata
    25621 gctcgcagtt tatcgggtat tttgtcatct gctacgtccg ttgcatcttt ggcatatgtt
    25681 ggagattcca gctcggagga agaaagaagc aagaatatga gtcaacttgg agcttcaatg
    25741 agcgttggcg taatgatggg accattattg ggaggcattc ttgcaggcta ttcgcttgcg
    25801 ctcccatttt ttacaggagc tggcatttca tttattgcgt ttctgctaat tctaacactt
    25861 ttgccagagt caattgagcg ttcaggacag acggaaaaaa gaaaaccctt tgatttttca
    25921 gacctgaaaa gtataatttt tggctcagca ggaatggttt tgatacttat tttcgtcgtg
    25981 catttctgtc aaacaggctt gcaaggaatt acggggctgt atgtcgtaga taagtttgga
    26041 ttttccacta agcaagttgg tgttatttgg atggtgctag caggtatttt gattgtagta
    26101 caaggctttt tgactggtcc tttagcaaaa aaaattggtg agaagcgact aatactgata
    26161 ggtatgtttt gcaaagcctt agtagcgttt gccatggtat tgacaactgg gtttgttagt
    26221 gtgttggtgg tttttggctt atttgcggta tcttctgcta ttacagtacc cacattaaac
    26281 gcaacccttt caaaaactga caatcagcat aaaggaacac tgatgggatt tgccagtacc
    26341 gccggaaatc ttagcaaagt tattgctcct ttattaactg gttatctgta tgaaagcaat
    26401 attgaattac catatataac cacgggaggg atagccttaa tcggagtggt tatctgctat
    26461 atctggataa aaaatcaaaa gaacaaatga tggaagagga cgtttatatg gaggttatac
    26521 acagtaacta atctggtata gaaattcaat tgaggctttt ctgctttatt aaagtcttgc
    26581 tactacttaa acctataata tccttaattc attatgacaa attccaattc aacagttttg
    26641 atacacataa ggaggtaaca atactatgga aaattatcaa tgaagtgcta attctttatc
    26701 agtgaagtta tccataagtt tttctacctg taatcgtctg gtgctttttg ccagatcatt
    26761 ggtaggtaag aaaaattcat caacagacac atgaagtaat gatacaaggt cacttgtatg
    26821 cttggatgtt gccctttatt ttcaatatta gttaagtaac gtgggtcaat atttgtaatt
    26881 cgattttagg gaaatattta aagaaatatt ttcgttttat ttctttaaat cttcaagaat
    26941 attgatagtt tttttgaaag taggtgtgta ttatgaagaa tgagaaaaaa attacagtcg
    27001 cattgctcat tgtttattta ttgattttat catggattat tttatttaag ctacaatttt
    27061 cattttctgt gttagaccac ataagacaaa taaacttaat tccttttggt ggttcagtta
    27121 ttgcaaatgg aaaaattgac ttcgatgaaa ttataaacaa tgccattgtt tttatccctg
    27181 taggtgcata tttcagtttg ctttttaaaa ataaatctgt tctaaaagca ataggttctg
    27241 ttttaggaat tagctttgta tatgagataa ttcaatttat atttgctatc ggagcaagtg
    27301 atattacaga cttaattagc aacacattag ggggtataat tggaattgca ttagtatatg
    27361 tattatctat tgttctaaag gataaaacac ataagatatt aaatcgaata gcaatagtat
    27421 gtactgtatt agtagttgcc ttcttattta tgcttcttgc tgtaaataat atattttaat
    27481 gccatgttaa ttacatgttg ttttggaaaa gactttaatc tgtcaaagtt tgcttttgaa
    27541 attgaacatg aaattacacc aatggaacaa atgaaagcta taaatttttc attggagaaa
    27601 gatgactgta caagtaaagt attaatatac aatccagaca aatggaaata tgtagaatac
    27661 tatttctata aagatagacc tattaaaaaa ctattggaaa aatttattca attttgcatt
    27721 tgtcacaata aaatatgttt tgaaatattg aatacttaaa tatgcaaaaa aaacaaatgt
    27781 aactggctat ggggctatat ctactaaaaa gctatgtgat aggttgggat taaatttaaa
    27841 catcacttct agaaggcatg aatacacaag gctgacaaac tatacagtta tgattaaacg
    27901 ataccaacgg gattgaaaat ttcgtgaaga attttgagaa gtcctattac acatgagcaa
    27961 cagcaacgaa gctattgaag caaggacgca ggcaatctag cggttatctg acaaaggagt
    28021 tgtaggactt gaatgtagat actatcagaa cggatatacc gaccagctct gcggttacag
    28081 atgtgattgt gtggaatatc gagcagtcgg gaacggacac tttatctgct acctacgaag
    28141 tagatcagca gataaaagag agagaacaga caacagcggt caaggcaacc tataccgtaa
    28201 aagtccatgt ggacgctgac ggggatatgg taatcgttca gaaccttact cttgcaccag
    28261 caatcgaaaa atcagactat gagccaaaga catcagaaac agacgcaagc gtggacgctg
    28321 ataccgttaa tgacgctacc gcatttctgg aaacattctt tgaggtgtac ccgacagcca
    28381 cagaaaaaga acttgcctat tatgtatcgg aaaatgtgat tgaacctatc ggcagggact
    28441 acctttattc tgaattggta aacccagtct ttacaaagga cggggacaat gtgaaagtca
    28501 aggtagctgt gaaattcctt gataatcaga caaaggcaac gcaggtgtcg cagtatgatc
    28561 ttgtactcca taaggatagc aactggaaga ttttaggata acatggaaat gggcttgtat
    28621 cataattata gtgtgcaagc ccttttcata ttgaagtatc aataatcaat aattataatt
    28681 aggtacatga atggagggat aaaatgttca aaattggtga gttttcaaaa ttgacacaag
    28741 tttctactcg tatgttgcgt tattatgatg aaacgggttt attaaaacct gctaaaattg
    28801 attcattaac tggatatcgt atgtattcag cagaacaaat ctcaacttta aatagaatta
    28861 tatatttacg tgatagtggc ttcaatgtcg ctgaaattgc attagccctc aatttcagta
    28921 ataatgccat tatagaacag cttgatagaa agtctattga aattatggat aatatacaaa
    28981 aagaacaaga aaaactaaag aaaattgaga ttgctaaaaa agaagtgtta catggcaaaa
    29041 gtgaattgta ttttaacata accattaaag agataccgag ttattgcgtt ttatctttaa
    29101 gaaaagaaat accaaattat tattatgagg gcgaactctg gaaagaactc tctgtttttg
    29161 ctaaagaaaa taaaataaat atttctgatg aaacattctc aatatatcac aatgaagaat
    29221 ataaagaaaa agatgttgat gtagaattat gtgccattgt aaataaaata ggaaaagcta
    29281 caggtgattt taagtttcgt tttaccgaac cagtttctat gatggcttgt actatggtat
    29341 atggagattt ttttaatatt tcttatgctt atcgttcatt tgctaaatgg cttcaagaaa
    29401 acagtcaata taagatgaaa ggttcaagcc gtcaaatagt tcatcgtgga ccgtggaacg
    29461 agaaaaatcc agagaactat ctaacagaaa tccaaattcc tttagaaaat ctcttgtaaa
    29521 aaatgataat ctgccttgac tttaacatgg tgttaaggtt tatcgtttat ttatcaagaa
    29581 gaaaaaggag gaaaatataa tgataactta taaagtgaat tcaataggta ctatctataa
    29641 taatgaaaaa ggtgctttca ttcaacttga agctgcatat atcccagcat taagggcatt
    29701 agacggattc agtcatataa atgttttatg gtggttcagt gattttgata atacggattt
    29761 tagaagtgta ttggaaacag aacagcctta taaaaatgct ccagagaaaa tggggatttt
    29821 tgcaacaagg tcgccagtac gtccaaatcc gattgcttta tcagtagtgg aaattatcaa
    29881 tatcgattat atgaatggaa tgatacaaat tgcttacatt gatgctaatg acaaaacgcc
    29941 tatcttggat attaaaccat acacaccgag tttggatagg gtggaaaatc caaatgttcc
    30001 agattggtgt agtcattggc ctcatagttt agaagaatcc gcttcttttg catgggaaaa
    30061 cgaatttaat ttttaaggag tgatgaacca tgagtttgca ggattacatt attgaaaaac
    30121 cagtaattga tacagaacac cttattttaa gaacaatgga taaaaatgac atagatgatt
    30181 tgaaagaatg gattagtgat atttctttat acgagtattg ggggaaacgt ccttcaaaaa
    30241 gcgaaaaaaa tccagaagta ctctttagta agccagaaaa agctactaaa agttttcatt
    30301 ggggaattgt ccataaacaa gatagcaaag tgattggtga aatatggata taccttattg
    30361 aaaataatag aatggcaaaa gtggctttca gactttctaa gcaatatcaa ggtaatggac
    30421 taatgagtga agcgttgaaa agcgtaattg aattttgttt ttcaaaaaca gaattacaaa
    30481 aaatatggac agatgtgcat attttaaatc atgcttctta caagacattg gagaaagtcg
    30541 gttttcaaag ggaaggacat attcgagggg gaaaaatggt aaatacctat tgcgactact
    30601 acctatatgg aattttaaaa tctgattata tcactagctg atgttatggc taatattgaa
    30661 gcgacgattg gcgaagcaat gaacaataca gttccaaagg tctatgatac gaaaagcatt
    30721 aaaagatatg tgatgaattt attagatatg gctcattaaa aagtaccaaa gaaataaaag
    30781 ttaccatata gacaaaattg gttttttata tcttttgatt tcttataaga taatggcatt
    30841 gtgcgacaag ctatacaaaa aattttcatg tatactttga ttataaatta tacaaggagc
    30901 taactatata taaatcaagt gatttttgat aattagctga aatatttttt agatcattga
    30961 aaagtgaatt ttgtgtacac tgaaaactac tgctttatgc agtactttaa aaatattcaa
    31021 ttgaatgctt ttaaataagc agtttttcat caaattgtat gttttcagac aacagtttat
    31081 agattactct tatgagtttt cgtacggaat gcccctgagc acatctatgg gatttacctt
    31141 gtaagcgttt tagtttataa tacttattga aagtgagatt gtatttgcat actgtaagaa
    31201 tgcattgata tagacttttt cttaggtagc gcgaaccacg tttagacaat gctgttcgag
    31261 gtgcactgta ttcaccggac tgataagtac cggggtctac acctgcaaat cccatgactt
    31321 tgacagcaga gctgaagtta tgaatatcac caatctcgct taaaatcgac attcccgtat
    31381 aaatcccaat accgggaatc gagaagatag gagaatctag tttgacagct aattcttcta
    31441 tttttttgtc tacagtttta agctgactgg ctaacagttc gatattttca ataagcattt
    31501 tgatttcaag ggtaacagct tcgttgtttt cgccgataga tttctttgca ctggaacgaa
    31561 gagatgatgc atctatattc acacggcttc cactgctttt aggcttgaga acttttttaa
    31621 gatgagcaag attggcactg gctaaattat aagaacttcc aaattctttt aagacagcca
    31681 tataagcttt ggaatattta gttttaaacc aagtattgaa ctcaggaata acgatatcaa
    31741 tacatttctg aagttggttt ttcttctggt tgatatcttc agcaatagaa gcatgataac
    31801 gggttaattc acgagcctgt ttcatatcga gcttacgctg agtgactcta gtataatctc
    31861 tatcaaagag tactgaacaa ataagaaaag tatctttttt atcattctta gtctttctta
    31921 ttttgctttt gcgcttggca tcagttgtta atggatttat caaagcaact tcataatcat
    31981 gagtgattaa aaaattaata agattatcac cgtaatgacc ggtagcttct aaaccgacaa
    32041 tatgtctagg tgatttaaat tttttaacag ctttgataaa agaatcgaat ccgtctagat
    32101 tattagtgaa gaaaaaaggt tcaataagga cttcaccttc agaagaaata acgcaggcac
    32161 agtgattgaa tttaccaacg tctataccga tgtaaaacat aaaaagcaca tcctttcttt
    32221 atataagata gtgatatgtg attaaaccac aaacagagta tcaaagagcc tggtgcaaaa
    32281 tacaaattcg aagacttatc taactgatca caattaagat acaaagatgt ggcaataacc
    32341 tccttcaagt agtcaagcta caggtgctga aagaaaaagt acacattcac aattcaattc
    32401 taaacaaaat ttaatgaaag gaaaaggtgt atataattga cagatagata agtcaattac
    32461 aatataccag gtactgatat gtacgcatat gaaaatattg aaatgtcatt aaactatatt
    32521 gaacaacatt taagtgagaa aatcgaaaca gagaaactgg cggaaattgc ctgtctttct
    32581 acattttatt accaacgctt gtttaaaaag ttagttaaga aatcagttca agagtatatc
    32641 aaacttcgcc gtttagcaat ggtaatagca gaattgaata attcagagga aagaatatta
    32701 gacatagctt taaaatatgg attttcagac catgctaact tcacaagagc atttaaagaa
    32761 gtttaccata ttactcccga tgaatacaga aagaatgttc ctatgttaaa tacatttgac
    32821 aagccagaac tttcttcacg ttatatcatg attgatgaaa atgtcccatt gattgtagga
    32881 aatattgttt tggaaattca aagaaaaaca ttaagaacaa aggaaacata ttttggtttt
    32941 gaaaaaacag taaatattgc tgaacaaatt cctgctggag aaagtacagg aattgatgtt
    33001 ccaggtcagc tatggcgcga attccatgca aagaaactgc aaaccgaaaa tgccattttt
    33061 aacaatgata ttgaacttgg tgtatctcac ttagcaagtc cagataaagg aacattcctt
    33121 tactttgcag gaggtatggt ttcatcaaca gatgtatgtc ctaaaaatat ggtacaacat
    33181 acattaccag caggtgagta tattgtatgc aaaattgagg cagaatcttt tgaagaactt
    33241 gttactactg ctttaaatca agcaaataag tatctgtttg aaacttggct tccaaatcat
    33301 aatttggtta caaagccatt catggcagaa aaatattaca aaggaaatgc agagattaat
    33361 tatatggaag tatggataat tcctgtgtaa acagtaaatg ctaaatagga attatctgac
    33421 gtaataaaaa ggaaatagaa agcttataaa ggagggcata gtccgttgtg ataaacataa
    33481 aagatataaa ttatgtacct aatattagtg aaataagcgt ttatattgaa aactctgcat
    33541 ttgatgagtt gtatcagcat atgaacaatg aatataaagc agtttgcaaa atcgaataca
    33601 gtaaagatgt ctgggcacga ggttggaacg taaagtttaa aaaatctggg aaatcattgt
    33661 gtgttgtcta tccgaagaag aactacttta ctgtattggt ggttgtaggt aataaagaga
    33721 aagaagatgt tgaaaacttg ttacctaggc tttctgaata tgtacaggaa atttatcgta
    33781 acactagaga gggaaatgga caaaggtggc ttatgataga cttaaaatct aaaaataatg
    33841 tatatcaaga tgtattaagg cttattgaaa ttcgttatgc cataaaatgt gtaaaaccgt
    33901 aataaaaata tttttcacat acgtaatctc aaggtattat cgttgagaca agtaacctat
    33961 ataattatgg gaaatgagat ttttagatac ttaagaaagt actttagggg gcttgcgtta
    34021 tggaaagatt taagtacaat tccacacaat caaatacaat ctaataaagc tttactaaaa
    34081 attgaatgca ttacagcttt aaaggctcgt acaaactata tgttttgtgc gggctttttt
    34141 catttaatag gaatttggat tcgtcagaga gtatattata aaaataaaac gataagggta
    34201 ccttaaaaat aaggtacctc aggctgtaga caaagtcccg gactctcgtc tgggcttttc
    34261 ttatatttga gttatccaca attcaacttt ttaagagtta tcttattaag aaaaaagtgt
    34321 atgttttcca cccgatagtt ttacttctgt gatatttgca gatgccactc aagtagaaag
    34381 tggtggtacg gcagaagatg tcatgcagag ttcagaaagt ctgggactac cgcctaattc
    34441 cttagacacg gaaagctcaa tcaagcaggg gtgtaagtat tttgcgtccc tgctttcttc
    34501 ctgcaaaaat caaggtatcg acgatttgaa tgtagcgata caatcttaca actatggcgg
    34561 gggttatgtg ggctatgtgg caggaaaagg gaaaaagcac acttttaatc ttgcagagag
    34621 cttcgcccgt gagaaatcgg gtggaaagaa agtaacctac gccaacccga tagccgttgc
    34681 aaagaacggg ggctggcggt atggctatgg aaatatgttt tatgtggaat tagtcaatca
    34741 gtatttaact gttccgcagg tgtcggggga actggcacaa aaggtaatga atgaagcgtt
    34801 gaagtatcaa gggtggaaat atgtgtttgg gggaagcaat cccaacactt cctttgattg
    34861 tagcggactt gtgcaatggt gctatggaaa agctggtatc tacttaccga gaacggcaca
    34921 aacacagtat gacgcaaccc agcatatccc tttatcgcag gcaaaagctg gggagttggt
    34981 ctttttccat tcaacctata acgctggttc gtatgtaacg cacgtcggta tctatgtggg
    35041 aaataatcag atgtaccatg caggcaatca gcggttatct aacaaagaaa ttgcaggact
    35101 tgaatgttga tactattaga accgatatac tgactagccc cacagttaca gatgtaattg
    35161 tgtggaatat cgaacagtcg ggaacggaca ctttttctgc tacctacgaa gtagatcagc
    35221 agataaaaga gggagaacag acaacaacag tcaaggcaac ctatacggta aaagtccatg
    35281 tagacgctta cagggatatg gtaattattc agaaccctac ccttgcacca gcaatcgaaa
    35341 aatcggacta tgagccaaag acaccagaag cagacgcaag tgtagacgct gataccgtga
    35401 atgacgctac cgcatttctg gaaacattct ttaagctgta cccgacagcc acagcaaaag
    35461 agcttgccta ttatgtattt ggaaatgtgc ttgaacctat cggcagggac tacctttatt
    35521 cagaattggt aaacccagta cttacaaagg acggggataa tatgaaagtc aaggtagctg
    35581 taaaattcct tgataatcag acaaaggcga cgcaggtatt gcagtatgag cttattctac
    35641 ataaggatag taactggaaa attattatgt aagacatttt gttggcagga tctattttcg
    35701 gatatagtct gccttttctg tagcaaatgt actgcactga caagctattt cattgtattt
    35761 taagcaatta aatctttctt gacaatttta tcttaaatca tatatttatg gatattgact
    35821 ttctgcttgc aataagttat aatatgacta taaaaccaaa ttagtcacat aggaggtatg
    35881 tggtatgtat aaagaagttg gtatttcttc aaataatgtt ccgataacac tttctatctg
    35941 gtatacagaa agaagtagtc ctaccattgt ttttttgccg ggtactatgt cacaccctct
    36001 catgtatgag aattttctat gtggtttgag tgaaagagga tttaatataa taggcattca
    36061 ctatctttca cacggaaaaa gccctaaaat caaaaaaaat tatacaatgg aagatttgct
    36121 gcataatgta tatgacgcaa caacttacgg aatagaaaat ttcggtgaaa acatagctgt
    36181 catgggttcc agtcaaggcg ggattttagc tgcaatggcg gcaggtaggg acacaaggtt
    36241 aaaagccgtt tttccacata atatcatgct gactacatta aaagaaacta tgagcttaac
    36301 aaaatatccc acatggtcgc accgttttat ggggctgata cagtggggct ttcgtgtagg
    36361 tggaaaattg ctgcctaact acaaggttcc aggagacgct tatttagact atgacagagt
    36421 attttatagt gaacaggcaa aacaagattg cttaaatgac cctatgcttt taccctatta
    36481 tcctttaaga tttgtcgcca gcttatttaa tgctgactta tcgtgcctgg agaatggggc
    36541 tatacaatgt cctgttattt taattactgc caaaggcgac ccgcttttta gctttgatta
    36601 taccaacaaa gtatttgatt taattcatgt accgcaaaag gaattacttg ctttagagct
    36661 taatcgtcac atgattttta atgaggacac ggacactaca ataaacgcat tggaagctac
    36721 cttgcataaa tatcttggat agcatattga ttatataatc agtaaatgtt atacttttca
    36781 tgacgatata aatattttag tcataaggag gttatgagtt tgccacgatt tacggaacaa
    36841 gaaaaagaaa tcataaacag taaacttttg atagagggag aaaagctgtt tgcattacac
    36901 ggcttaaaaa aagtaacagt tgatgattta gtagctgctg taaacatatc aaaaggttct
    36961 ttctatgctt tttatcccag taaagaacat ctatatgttg aaatcaattt tcgcttacaa
    37021 aaagagttat ttacaagtat agaaacaacc attaaaaaga aaaagtataa gaaccataga
    37081 gatttagcaa aggacgttat catgctaagt ttaacggggg tgattacttc gcctattctt
    37141 tcacagattg atttatcttt aatggactat ctgcaaagaa aaatatcgtc agatattttt
    37201 gaaaatcata tgtacagtga tattcgtata ttggagatat tggaagattt aggggttgta
    37261 tttttggttc cgcatacggt tattataaaa tccctgtatt ctgttttatc ctgtttggaa
    37321 caattcaaag aggacgaaga actgaatatg atacaaaatc ttttagtgaa tggtattatt
    37381 cagcaagtag tagcagagta acaattttgt ttggttattt gacaggcagg tagccaaaca
    37441 aattttttac agtttatatg actatatatc tataagcgtc ataaagttaa aggaggaatg
    37501 aataatgatt gatgtaaaaa aactgtattt tagctatact gacaaaccgt ttgtggagaa
    37561 tgtaagtttc catgttggga gaggagaaat ttttggtttt ttaggaccct ctggtgctgg
    37621 aaaatcaact attcaaaaag ttttaactgg gcttaataca agatataaag gcagcgtaaa
    37681 agttgcaggt acagaaataa gggagcatac aaatcagttt tatgaaaata ttggggtaga
    37741 ctttgaattt tcaacctgct acgagaaatt cacagcacga caaaatctcg cctattttgc
    37801 ttctctatat gaaaagcaac ctcggtctat tgatgaatta ttgcatatgg taggcttgga
    37861 aaatgacggg gacaaaaaag ttgctgactt ttccaaaggt atgcgttctc gcttgaattt
    37921 cattaaagcg ttagtccatg accctgatat attattcctt gatgaaccta caagcggtct
    37981 tgaccctaca aatagtcgtt taatgaaaga tattattctt gcagaaaaga aacgcggaaa
    38041 aacgattatc attactactc acaatatgtt tgacgcaaca gagctatgtg accaagtagc
    38101 ctttatcgtt gccggaaaag tcagtgcatt ggatagcccg cataatttga ttatgtcaag
    38161 gggagctgcc aaaatacaat atacctacta tgataaaggc gagaaaaccg gagaatgtct
    38221 gcttgataga accacagaag ataagttgtt aaaaagttta atttccgaaa accgtttgct
    38281 ttctattcat agtagtgagc ctacattaaa tgatattttt gttgatatta cagggaggac
    38341 acttcaatga gattgggacg tttaatttgt ggtgatatac atttccaatg gaaatatggt
    38401 ttctatttta tctactttat attaacagtt ttatatgtgt gcgggattgc cgctttgccg
    38461 gaacattgga aaacggatat tgcttctatt atgatctact ctgacccggc agcaatggga
    38521 ttatttttta tgggagcgat tgtcctctta gaaaaaagtc aaaaggtgtt aaatgctatg
    38581 gtagtatcgc ctgtaaaagt atcggagtac atactttcaa aaacaatcgc gttgattgct
    38641 atttctacgg taatcgcatt gatattaggg gtagtttccg acagcaatca tttacttggt
    38701 atcacagttg gtacggcttt gacctcagca atctttacta tgcttggaat tattgcagct
    38761 acaaaaattt caaacttgaa ccaatttctc attgtgatta tgccgataga aattgtttgc
    38821 tttgtaccac ctattgttgg tctgtttgtg aagttaccat atctgttccg cttttttcca
    38881 ttcacagctt gcatgaacct tataactgga aaaagcgttt tactatcgtt tgatatggta
    38941 cttgtaattg ctacgttgat tatcctctac atagttgcgc gacatacagt taagcatatg
    39001 tggaaaagtt taggaggtgt aaagctatga aaaggatatt atccagtacc atacaggtat
    39061 ttagacaaat caagagtgac cctatgatgt ttgcagcttg ttttacgcct tttgtcatgg
    39121 gagctttgat taaattaggt attccctttc ttgaaagaat aacggatttt tctttgcaag
    39181 catactatcc gatttttgat ttactacttt ctatcatggc tcctgtatta ctctgctttg
    39241 catttgcaat gattacatta gaggaaatcg acgataaagt gtcacggtac ttttcgatta
    39301 cccctcttgg taagtcgggg tatctcttta caaggttggg agtaccctca attatttcgg
    39361 cggtcatcgc ttttattgta ctgttacttt tttcattaga aaagttgtcc attggaatga
    39421 caatatgttt ggcgcttctc ggctcagtac aggcaattat tgtttcactt atgattatca
    39481 cattatcaag caataaatta gagggtatgg cagttacaaa actagctgca cttacattgc
    39541 tcggaatacc agcccccttt tttattgata gttactacca gttcgcggtt ggcttccttc
    39601 catcattttg ggtagcgaaa gccatgcaga atgaggcggt tctttatttc tctataggat
    39661 tggtggtagc tttagtctgg tactacttcc ttacaaaacg tctgtttcgg aagctggcag
    39721 gataaggagg catattatgg aaaagggtag acttctgcaa aacggaaaag taaatagagc
    39781 tattcaggtt tccaaaatca cactatttat caacctaagt ttatcgttac ttaaatttac
    39841 tgccggatat ataggaaagt caagtgccat gctgtcggac gctgtacact cggcttctga
    39901 tgtattaagc acaatcgttg tgatggttgg aattaaaata tcagaaaaac agcctgatag
    39961 agaacatcct tatgggcatg aacgtatgga atgtgctgct tccattattc tctctgttgc
    40021 attggccata acaggtgctg gaattggata ttcaggtatc aagaaaatct tttcagaaca
    40081 gtacaatacg ctttctactc ctagtggaat cgcacttacg gcggcagtct tatccattgt
    40141 tataaaagag tggatgtact ggtttacaag aaatgctgca aagcatacaa attctgacgc
    40201 acttatggct gacgcatggc accaccgctc tgatgcactc tcgtcggttg gttccttaat
    40261 tggcatttta ggagcgcgtt taggctatgc tattctcgac cctattgcaa gcgttgtgat
    40321 ttgcggttgt atcttaaaag ctgctttgga cattttcaaa gaaagcatta acaaaatggt
    40381 agaccatagt tgtgacaatg ctactgaaac aaagattagg gaagttgtgc ttcagcagca
    40441 aggggttgat ggtattgatg aattaaaaac ccgcatgttt ggagcgaaaa tgtatgtaga
    40501 tattgaaata ttagctgatg gtaatttagc tctttatgac gctcatagaa ttgctgaggg
    40561 agtccatcaa acgattgaaa acaactttcc acaatgtaaa cattgtatgg tacatgttaa
    40621 tacgaatgaa cttctctatt ctgcatagga cagggaagat gatttaaggc tattgaaagg
    40681 gaaaggaggg aagtaggtta tggaacaata ctggtggatt atcattgttg tggttttgat
    40741 tgtcgttggt ttacttaaaa ttgtaattat gaagaagtat aaccaaagga gaaattctaa
    40801 caatagtcca aaagatagcg aggacgacaa gatgtattaa ggtttattga aatctgttat
    40861 gttgtagaat gatgttttta gatactagaa agtgctttaa gggggctaac gttatggaaa
    40921 gctttgagta caattctttc caatcaacag tattttgcat agagaaatgt atgtaggaaa
    40981 tatggtgcaa ggcataaagc accagcgatc gagagatcaa cctatgaacc aaaggcacac
    41041 tacattttat actacttgac aagtaaacga gcgtttacta taatggttta cagaagtaaa
    41101 cgttcgttta ctaaagaagg aggttgtaaa tgtctgacac aaaagaaaat atattaaaaa
    41161 ttgcactaca cttatttgca gtgaatggtt atgaggctgt ttctgttagt atgattgcgg
    41221 aagccttggg aatgacaaaa ggagcattat ataaacatta taaaaataag caagatattt
    41281 ttgactgtat cgtagaacgt atgtatcaaa ttgataataa gcgagctaag gattatcaag
    41341 taccagaaag cacaatagag gtaaccccta atgcatacag aaatacaact attggaagtc
    41401 taataacatt tactcttgca caatacgatt tttgggtgac tgatgaattt gcgtctaatt
    41461 ttcgcaaaat gttaatacta gagcaatata gaaatttaaa agtgaatatg ctttatcaaa
    41521 aatgtcttgt aaatggtcct atcagttata tagaagatat atttaatgaa atgattgaaa
    41581 aaggtgttct tcaagagtgt gatacaaaac agcttgctat tgaatattat gcgccattat
    41641 ttctattgat aagtgtagca gacgcagttg aaaattgttg ggatttaaaa actcaattaa
    41701 ctacacacgt taaacaattt gtcaagaaat atattataaa tgagaaagta aaataatcct
    41761 ttattctgtc ctaaatataa acctaacttt ttaatttcag cagagaaact caaaatgaag
    41821 attgttataa gtcacaaata ttaactatta aaatattaca ataacggaga tgagaaaatg
    41881 gaatatattc gcagtaaaaa atattttaca cccgacttac agacaaaaat tatggggccg
    41941 aatcctataa aattacaaga ggaactatta caaaatcata aaatcccgtc tggtagctat
    42001 gtctgtgacc ttggaagtgg acaaggttta acttctgtat ttcttgcaaa agaatatgga
    42061 tttgttgttt ttgcagttga cttatggagt gagccaaaag aaaatgaaga atttttcaaa
    42121 aaaatgggat tatcttctgc acaaattaaa tctgtaaaag ctgacgccaa tgaacttaaa
    42181 tttgagaaag agttttttga tgctgtagta agtacagatt catataatta ttttggacgt
    42241 gaccctatat atttagatga aaaattgctt ccatttgtta agaaaggtgg gtttgtttat
    42301 atcacagttc ctggcatgaa gaaagattgc catgagaaac ttccagaaga gttattgtta
    42361 tcatggacac cagaagaact tgattatata catgatataa catattggtc taacataatt
    42421 agtcattgta aaaaggcaga tttaatttca atatatgaaa tggaaagtaa ttcggaagtt
    42481 tggtcggatt ggataaagca aaataatgaa tatgcaataa atgaccgtaa ggcaatagaa
    42541 gctggcggtg gagattatct gaattttatt gctataattc tacaaaaaaa ataaattctt
    42601 tagaaccaaa cactataatt tgcgttctaa tatgtttacc gattgacatc aataatttta
    42661 tttttgaggg taatgatttg aaaatagaag aataattcca gtttgtaaaa gtcaataaat
    42721 ccaaagataa atttgtattt gaaaggagtt ttgaaaaatg aaaaaatatg agtatgtaga
    42781 aattaactat aatgcgaaag gagttgtatt cctttgtact gataaacaca aagaaattat
    42841 tgacagttac gcaaaaacgg ggtatcacta cgcagggttt gtacccacag aaattgacgc
    42901 aaaaggctgt atgcgaaaga tagatttaat ttttgaaaaa gaagattaac ttattttagg
    42961 gagttggttc taatgatagg acttaaaaga cgaaatatga attatattat gaaagttggt
    43021 gctaaagctc aaggtgtttc tgtatctgaa tttagggcta tgctacaaga taccatagac
    43081 aaaactatgg aaagtacaga cccagacgtg caggcaaatt ttaaacgata ctttggaaat
    43141 aagcgcccaa caccagagga atatatttat acgattacca aaaagacaaa ggttaaggtt
    43201 taaatgttgt aaagggggct tgccgtatga aacgctttat gtacaattca gtatattcaa
    43261 gcccaattta acagcatact atttacaatt aaatacgcta taccagtagc tcgtacaaaa
    43321 tttatatttt gtgcgggctt ttttaatacc cgaaaagttt ttttaattat tttcattttt
    43381 ttggaagcaa atcacacctt gctgtacaga tatggtgcgg aaagagggat aaacactttt
    43441 caattcatag ctggaaagga ggtgagatta tgaaaccgtc tgacttccaa aaaacaattc
    43501 agtgtcagtt tgaatgtaag ctgaaaaaag ttgtcaaagg tattatctgg aactaccgta
    43561 aagaattaaa acgaagaaaa aacaaggaaa tatcttttag cgaattacca gacattacgg
    43621 tagaaagtct ggcaacatgg gacgactacg aaacaaacta cacaattttt aatgtgtgtg
    43681 gcatagatat taaagtcctt gatgatgaac ttgcagaagc tctcaaacag ctacccgaaa
    43741 aaaaacgtaa caatgtattg atgtattact tcttggaaat gagtgataca gagattgcag
    43801 atattatgaa ttataccaga ggggctgtgt tcaaacacag acaccgtgcc ctagaaacta
    43861 tgaaagagat actaaaggag aagaaataga aatgaagaaa aagtacaagc acccgtctta
    43921 ttatttgatt tcatcagcta tggacgggga tgaaactgcg attgagaaat tactggtatt
    43981 ctatgacccg tacatatcaa agtgttgttt acgtccttta tatgatgaat acggcaatgt
    44041 ttatattgtt gttgatatgg aattaaaggg acgtattcga gaagccttaa tcaaaatgat
    44101 tttagacttt gagattgata tagatataga aaccgaagaa gaataagcag ataccagcag
    44161 ggcgtgattg gttaaacctc ctctttcctg ccctgctcct gcttttatac atggaagcct
    44221 acggctttcg ccgattgttc tttgacaact gaataaagca gacagatacg tttgtgatac
    44281 agcgagccac ggggactgta cgccatgacc tttccgacaa gaaagtgagc gacccacgca
    44341 gtgatccgag agcaacttgt ggaatggtcg ctttgccatg acctgctatc acagaataat
    44401 gatacgtccg catggtgcgg ttctgcccac aagaatggga atagttgaga tactaacgga
    44461 gctttccgaa gccgtctgtc tgcttatgat aagacataca gaaaggagtg ttgcaacatg
    44521 aacaatactg atgtgcctat ctgggaaaaa tataccctta gtatagaaga agcgtcccga
    44581 tattttcgta tcggggaaaa gaagttgcgt aggcttgccg aggaaaatct ggactctggc
    44641 tgggttatcg tcaatggaaa ccgtattcag ataaaacgaa aacagtttga aaaaattatt
    44701 gatacactgg acgaaatctg atgtcttaga gccgtagata tggtataata ttagtagcgt
    44761 atcacggctc attccatgac ggaaaggagc tttacaccat gtcaaaagtg aaaagacaag
    44821 acagtaaagg acgcaatttg cgtcttggag agagccagag aaaagacgga agatacgttt
    44881 ataaatatac cgatatattc gggaagccac agtttgtata tgcttggaaa cttgtaccca
    44941 cagacaaaac acctgctgga aaacgtgatg atatatcgtt gagggaaaaa gaaaagcaga
    45001 taaaaaaaga ccttgacgac ggtatcgaca cagtgggtgg taaaatgaca gtctgccagc
    45061 tttacgaaaa gaagaacaat caaagaaaga acatcaagag aaacacaaaa ataggacgac
    45121 agtatcttat gaacgcactg gaaaatgacc cattgggtat gagggcgatt gatacggtca
    45181 agcagtccga cgctaaagaa tgggctatca gaatgaatga caaaggttat tcctacaaaa
    45241 cgatagataa ctataagcgt tccttaaagg cttcattcta tatggcgata caagacgact
    45301 gtatcagaaa aaatcctttc aatttcaagt tgagtgatgt tctggaagat gatacggaag
    45361 caaaggttat cctaacacca gagcaggaag aaaaactcct tgcctttatg gaaacagata
    45421 atgtctacag caagtatcgt gatgaagtta ttcttctgct ggaaacggga cttcgtatct
    45481 ctgaattatg cggactgaca acacacattg atatgcttaa ccgtgtgatt aacataaacc
    45541 accagctatt gcgtgataca gaggtgggtt actatatttc cacacctaag acaaaaaacg
    45601 ggaagcgtaa gcttccattg acagaaagag catatcaggc acttgaacga attttaaaga
    45661 accgaggaaa agcacaaccg cttgttgttg acggttacag caacttcttg ttcttgaacc
    45721 gtgagggatt gccaaaggtg gcaggaaatt atgaaagtat ggtgcgtggg ctgattaaga
    45781 agtacaacaa gacgcataag gacaagttgc caaacatcac gccccattca ttcagacata
    45841 cttactgtac gaacatggca aataagggaa tgaaccctaa caccctgcaa tacatcatgg
    45901 ggcacgcaaa cataaccatg acattagggt attatgctca cggtactttc cagtctgcaa
    45961 aggctgaatt agaaaggctg gcttcttaat atcgaaaatg tatcttacta cttatttact
    46021 acttttggtt gcattttcat gctggtaaat gccatcttat gcgaggtatc ttccaaaagg
    46081 aaaatgccaa taaagcccga aaatatggga tatatcggca tttaccaact tatgaaaaga
    46141 taatcagaaa tttagtaagt ttttatatca aaaatagtaa taaagtctat atacatttag
    46201 aaaatactgt ttaaaaggag taggatttat gataaaaaaa gagatatcta actttagagg
    46261 aagtagtgat tatgtagtat caccagaatt gatggcatca gttaatgttg ctattgctct
    46321 tgaaaagcca cttttgatta agggcgaacc tggaacaggt aaaactatgc tagcacaagc
    46381 aatttcaaat gaattaaaaa aagatttagt aatttggaat ataaaatcaa ctacaaaagc
    46441 acaagaaggg ctttatgtat atgatactgt tcaaagactt tatgatagcc aatttggagg
    46501 agaaggtgta gatgatatta gcaagtacat aaaatatggt aaactaggag aggcctttag
    46561 ctcaaatcaa caagtaattc ttttaataga tgaaatagat aaggcggatt tagaatttcc
    46621 aaatgacttg ctttgggaat tggataaaat ggagttttac ataaatgaga caaaagaaac
    46681 tgtaagagct aaacaaagac caatagttat tataacttca aatgcggaaa aagaacttcc
    46741 agatgctttt ttaagaagat gcatatttca ttatatagaa tttcctgata gagacatgat
    46801 ggaagaaata gttaaagtac attttgataa agtagaagaa cacctattgg aacaagtaat
    46861 gactactttt tattggatta gaagtttaaa ggacatacaa aaaaagccta gtacatcaga
    46921 gttaatagac tggatacaag cattaacttt gagtggaatg cctattgaaa aaatagaaaa
    46981 agaagttcct tttgcaggaa tactacttaa gaataatgag gatattgaat ctatgcaaag
    47041 acacttatag ttaataggag ctggataatt atgtttattg aattttttta tttattgagg
    47101 tcaagaggat taaatgtatc tttaaatgaa tggatgactt taattgaagc attggataaa
    47161 ggactgtgct attcaaactt ttcaaatttt tactatcttt gcaggatgat tttaataaaa
    47221 agtgaaagtg attttgataa gtttgatgca gtgtttttgg agtattttaa aggtatagaa
    47281 caccaagaag aaattccaga agagattatg aattggctta aaaaaccaga tatagattta
    47341 gaagagtttg aaagattaga acaaaatcca aatataaatc ttgataaatt aagagctaaa
    47401 ttggaggaac gaataaaaga acaagactct cctcataatg gtggtaatta ttggataggc
    47461 acaggaggca cttcagagtt aggacattca ggaaaagggc aaactggtat acgtataggt
    47521 ggaaattcta cttatggtag agctgttctt gaagttgcag gagaaagaaa atacagagat
    47581 tttagagatg atgaagtttt aaatatgcgt caatttcaag ttgcactaaa gagacttcgt
    47641 caattttcta ccagaataga tgctccaaag acagagcttg atttggataa gactattgaa
    47701 gagacttgta ataatgcagg atatttaaag ttattttttg agaagccaag aaaaaataca
    47761 gtgaagcttt tattgcttat ggattcaggt ggttcaatga gagggtatag cactttatgt
    47821 aatacattat ttcagtctgt aagtaaatca aatcatttta aagatgtaaa gatatattat
    47881 ttccataact gtatttatga tagattattt acaacacctg aatgttggtt aagtaaatct
    47941 attaatactg aatggatatt aaaaaatata gataaaaact ataaagttat aattgttgga
    48001 gatgcttcta tgtcgccttc tgagttgctt catattggtg gtaactatcg tggtccatat
    48061 aattacacac ctggtataga gtggcttaaa aggtttaaga gaaagtacag taaaattgta
    48121 tggatgaatc cagaacttag agatggatgg gattcaatta attattggta tcaaactcaa
    48181 agaatgatac aaagtgaatt taatatgttt ccattgactg taaaaggtct tgagaaagca
    48241 ttgaaaaatt taatggtttc aagataaagt aaatataaat gatggtatga tttattcaaa
    48301 attgtaccat cattatttat tttttttaat atatctatta taaattgtat aactgaaact
    48361 taaaaaatta tatagtctaa tacattttag actcacacaa aacatataca ttaataataa
    48421 ttttagaagt cgaggtgatt ataataaatt atgaattaat tgtaaaatac aatggagata
    48481 tattgaggtt agaagaagaa ttaggtgtat cagtagagat acttaattca tcatatgcaa
    48541 taataacatc aagtaatgaa gaagatgtaa atatactttt aacttatcca gaaattgaat
    48601 ttatagaaaa gccttttata ttacagactc aggatgtgca aagtttttca agtacaggaa
    48661 taacaggatt taaaaataga actggtttaa ctggaaaagg aactataatt ggtataattg
    48721 attcgggaat agattatact ctacctgtgt ttagagatag tgatggaagg tctaagatat
    48781 tatattattg ggaccaatct attcaaggaa atccaccaga aggttttaga gaaggaacat
    48841 tatatactaa tgaagatata aataatgcaa tagatggaag tatgtatata cctatttcaa
    48901 caactagctt acatgggaca catgttgcag gtatttgtgc aactattgca agtgatgcca
    48961 gaataatagt ggttagagtt ggaaatatac agaccgatat tttttcgaga agtacagagt
    49021 ttatgagggc aattaaattt attttagata gggcattaga attaagaatg cctgttacat
    49081 taaatataag ttatggaagt aatgaaggtt cacatagagg aacatcttta tttgaacaat
    49141 atatagatga tatgtgttta ttttggaaaa acaatatagt tgtagcagct ggaaataatg
    49201 cagataaagg aggtcataaa aggattaggc ttcaaaataa tattacagaa gaagttgagt
    49261 ttatagttgg ggaaggagag cgtatattaa acataaatat atggccagat tttgttgatg
    49321 attttagtgt acatttggta aacccatcaa ataatcaaac acaagcaatc tctctaactt
    49381 caggtgagat aaggaatact ttgggtgaaa ctagaataac tggttatttt taccctatag
    49441 caccgtattc tctcacaaga agagttactc tacaattaag ttcaaataca cagataactc
    49501 ctgggttatg gaaaattgta tttgaaccaa tagatatagt aactggtaat gtaaatatat
    49561 atttgccaac ttctgaaggt ctgaatagaa atactagatt tctgattcct actcaagagc
    49621 ttactgtaac agttcctgga acagcaagta gagttataac agtaggtagt tttaattcaa
    49681 gaacagatat agtatctata ttttctggtg aaggagatac acaattaggt gtttttaaac
    49741 ctgatttatt agctccaggt gaagatatag tatctttttt accaggaggt acaagtggag
    49801 cattaactgg aactagcatg gctacacctc atgttacagg ggtttgttca ttgtttatgg
    49861 aatggggaat tgtaaatgga aatgatttat ttttgtattc acagaaatta agggcattac
    49921 ttctaaaggg agcaaggaga ttaagtaatc aatcttatcc aaataattca tcaggatttg
    49981 gttttttaaa tttatcagat atagatttgt acactctatc taatataaat caagatttag
    50041 aaacagagga tattggatat agaagtatta ataagtcatt taaagatgag gaaaatagtt
    50101 ataagtttat agatggttat aatatgcaaa tacataatga cttagagaat gaaatttata
    50161 taagtaaaaa tgcaagtaga caatcaggaa tactatcagg aatagatata gtccatactc
    50221 ctgaatttga agaagaacta gctgggttag gtatgagtca aaggtttttt aagataagtg
    50281 attcattagg tgtactatct ataaataata cagactataa tagcatacaa cgagttttgc
    50341 aacttccttc aatcataaga actgtatcga ctacaaagat gacattactt ggagaaatta
    50401 atcgaggtac ttttggaggt gtagttgcaa cagaagaaat gggcgtaaat ttctttaaaa
    50461 ataatcctaa tattaatata acaggaagag ggactctaat atcaattgca gatacaggaa
    50521 tagactatct acacccagat tttatttatc cagatggcac ttcaaaaata gtatatttat
    50581 gggaccaaac aaaagaagga actcctcctg atggttttta tattgggaca gaatatacac
    50641 gagaagatat aaatcgagct atcgcagaaa atgaccctag tctatctcag gatgaagttg
    50701 gtcaaggaac tatgttgagt gggatatgtg cagggcttgg aaatgtaaat agtgaatatg
    50761 caggtatagc agaagattct gagctaataa taataaaact cggcaagata gatgattttt
    50821 ataatagtgc aatgttgttt gctgcatctc agtatgcata taaaaaagct tttgaacttg
    50881 gaagaccatt agttataaat atgtctttag gtaccagtag cctagcaggt ttaaccaata
    50941 ggagtaatag tgaaaaagcg ttctttacta gagggttatg tataacggca ggagcaggaa
    51001 atgagggcaa tacacagaca catacatctg gaataatacc atatgttgga ggttctgttg
    51061 aagttgaatt agagttaaat gaagacgagg aagaattatc attagaactt tggttaaata
    51121 gaccagataa agctgatgta ataatagtat ctcccactgg tgaagaaagt aaaagtgttg
    51181 gaatttcaaa ttataataaa gtaacaggtt tatttgattt agaggggact gaatattcta
    51241 taacatatat atacccaaca actttttctg gacagcaatt tactaatgtt actttaaaga
    51301 atgcaaaaag aggtgtatgg aagattcgat tagtaggagt ttatattata acaggaagat
    51361 ataacttata ccttccaaat agagaacttt taaaaagtgg aactagattt agagaggtag
    51421 acccttttta cactataaat tatcctgcga ttcaagatga tttaataacc ataggagcct
    51481 acaatactat taatggtagt ttatggcaaa gttcatcaag agggccaact attgaagaca
    51541 ggttaaaacc agatatagta gcaccaggtg tcaatataat agctgcttat cctgggaaca
    51601 cgtatgcaac tataactggt acagcagcag caagtgcaca tgcagctggt gctgctgcaa
    51661 tgtattttca atatactttt gttgacggta gatatccaaa ccaagcatat gtgcaaaaaa
    51721 taaaaacttt tatgcaagct ggtgctagaa aagattcaaa cacagtatat ccaaatacaa
    51781 attctggata tgggctcttg gatgttagag gaatgtttga tgttttaaga taggagggat
    51841 ttatggaaaa atcttattgt ataatttatc aaggtgatat agaatctgcc ctacaagaaa
    51901 atggaataaa taggtatatg gttttaaata gtcagcttgc agtaatatat gtacctttag
    51961 attttgatga aactatttta aataatataa tacaagttgc ttggtgggaa gaatctgaac
    52021 caatgagcag tctaattgaa ataactaata atgtaaataa tggagaaacc attacaacag
    52081 cagctgaaac tgattatatt tatgaaaatc catataatga tataacaggg agaggaattt
    52141 tattagcagt tatagattct gggatagatt atttgcatcc tgattttata aatgatgatg
    52201 gaactagtaa agttttatat ttatgggacc aagaagcaaa tacaaatcca cctccagaag
    52261 gttttatttt tggaagtgaa tttacaagaa gtgagttaaa tatagccata aatagaaatg
    52321 atgggagttt gagtcaagat aatataggta ctggaacttt agtttctggt atattagttg
    52381 gaaatggaag gataaattct cagtacagag gtattactac agaatcagac ttaatagtag
    52441 ttaaattaaa atcatataca gatacttatt atgctggaag gataaattac agtgtttcag
    52501 attttttagc agctataacg tatgttacta acatagcaag aacagaaaat aaaccactga
    52561 taataaattt gactattgga gttaagtcaa gtgctgtagc aactacttca atactagata
    52621 cttttaatat attgagtagt gctggtgtgg tagtagtgag tggtgcagga aatcaaggga
    52681 atacagatat tcattattct ggcagattta gcagtgtggg agaagttcaa gatgtaataa
    52741 tacaagatgg agatgactat gctcttgata taacactaaa tacgaatggt ccagataaag
    52801 tgggagcaca gataatatct ccttcaggtg aagttagtca tgatataagg tattctccag
    52861 acttttatat atatagagga aaatttaact tagaaaatac tacttatgca atgagattta
    52921 tttatccata tattacctct ggaaaagaaa atttggagat aagattaaga gatataaaac
    52981 ctggagtatg gattttaaga ttgacttcag aacttataat aaatggagaa tatgacatat
    53041 acttgccaaa taaaaatttg atagctccag acacaaggtt tttagaccct gattcagtag
    53101 ctactataac tatgtatgct gctagtgatg atgtaattac agttggtaca tttaacaata
    53161 agactgatag tatgtggatt ggttcttcga aaggaccaat tagaggaaga ggaattaagc
    53221 cagatatagt agcttcaggt gtagatataa tatccacata taaaaatgga acatataata
    53281 caggaacagg aacaggagtg agcagttcta tagtcacagg tgttttagcc ttattgatgg
    53341 aatacttaga aaaacaggat aatgtaccga gattatcatt gtttacacaa gttttaaaaa
    53401 catatttaat attgggtgct acaaaacttg agatatatac atatccaaat gtatcgcagg
    53461 gatatgggat tttaaattta aaaaatacga ttcaacagat agcaaatact ctatagataa
    53521 gaacctatgt aaaattataa ttgattatta gtttaattgt atcaaatact ttagatagag
    53581 tagaaaaata gcataaaaaa taaatatgaa aattaaaaaa cagttttata ttttgaatac
    53641 acaaagtaaa tattttgtat ataaataggt ataaattaag tattgaaaag atatgatgtt
    53701 aatttaaaaa taattataat ttagacttat gaattttatt tgtaaaattg ctaaaactat
    53761 tacaaaataa ataatagagg tgatagtttt ggaaagtaaa aaggcaagag cattaaaagg
    53821 taaagataat aaaagactta aaagttcact tttagttgga aatgagtcga cagcagcatg
    53881 ggctgatgtt gaaaaattaa aacctcatag taaagtttca ataccttcaa ttaagggtgt
    53941 cgaagaagca aaagactggg ttgacaatgg aagtaaatta taaaattttg gaaattgaag
    54001 taataaagaa gtaatgaaga taagtattta ttatcattat gtaaggtaga attattatat
    54061 tttaaataac aatcagtatc aattatataa atttattgac taaaaattta taaattggta
    54121 taattttgtt aattagcaga aataataaaa gataaatact gtgaagagaa gagtagatat
    54181 aaaatgtagt tctagcgagt tgaggatggt gaaagctcaa tacgaggttt atattgaaga
    54241 acatcttgga gtttctaatc gaaagcttat ttagtaagta gacttagacg gaaggcaccg
    54301 ttataggctt taagcattgc ttaactaaga ggtcattatg acaactaggg tggtaccgcg
    54361 agagtaatag tctttcgtcc ctttatatag ggatgaaggg ctttttttat tgaaattata
    54421 tttaaaagaa attaataaca attatactag gaggatttaa aatgcaaaag gaatttataa
    54481 cagtaaaaga attgtacaga gaaaaagaac agtacatagg gaagactgta aaagtagctg
    54541 gatggataag aacatctaga gtatctaaaa attttggatt tatagagcta aatgatggaa
    54601 gcttctttaa aaatatgcag atagtatttg atgagaaagt agaaaatttt aaagaggtag
    54661 gaaaattagc aataagctca tctatacttg tagagggaga gttagtatct acagaagggg
    54721 ctaaacaacc agtcgaaata catgcaaaaa gcatagttgt agaaggggaa tcagatagtt
    54781 cgtatccact acaaaagaag agacatacgc ttgaatattt aagaacgata gcacatttaa
    54841 gaccaagaag taatactttt tcagctgtat ttagagtaag atcaatagct gcctatgcaa
    54901 tacacaaatt cttccaagaa agaaactttg tatatgcaca ttctccaatc ataacaggta
    54961 gtgactgtga aggtgcagga gagatgttta gaataacaac tatggattta aatgatattc
    55021 caaaaactga agatggcaaa atagactata ctcaagattt ctttggaaaa gaggctaatt
    55081 taacagttag tggtcaatta aatgccgaga taatggcact ttcttttaga aacgtttata
    55141 cttttggacc tacatttaga gctgaaaact cttatacagg aagacatgct tcagaatttt
    55201 ggatgataga gccagaaata gtttttgcag accttgaaga taacatggaa cttgcagaag
    55261 atatgataaa atatgttata agctatgtat tagaaaatgc tcctgaagaa atggaatttt
    55321 ttaatagttt tatagataaa ggattgttag aaagattaaa taagatagta aactctgagt
    55381 ttattagaat aacgtatact aaagcagtgg aattattaat tgaatctggg catgaatttg
    55441 agtatcctgt tgagtgggga tgtgaccttc aaacagagca tgaaagatat ataacggagc
    55501 aaatatataa ttgtccagtg tttgttacag attatccaaa agatataaaa gctttctata
    55561 tgagaatgaa tgaagatggt aaaactgtta gagcaatgga tttactagtt cctggagttg
    55621 gagaaatcgt aggaggttct caaagagaag aaagagaaga tgtattgtta gatagaatta
    55681 atgaaatggg attaagagaa gaagattatt ggtggtattt agaacttaga aagtttggta
    55741 ctgctactca ctctggattt ggtcttggat ttgaaagaat aattatgtat atgacaggta
    55801 tgtctaacat aagagacgtt atacctttcc caagaactcc taaaaatgct gaattttaat
    55861 aattatatat aataaaactt atataacttt aagtataaac actaaaatga cttgcatatt
    55921 tatctatttt agagatatgc aagttatttt tattgtacta taaatatata atttgattac
    55981 ttttattttg cgatataata cttataggtt tatgtaatag tatgtaacct tttaataaaa
    56041 tgcaacacaa ttacgtcctt gttcttttgc agtatatagg gccttatcac aattacttat
    56101 aaactcattt aatgttattg tctttccaat tttctttgta taaactccaa tactaacagt
    56161 tacacatttt aaaggaacat catcacgctc aaaattgcag gactctataa tttttcgtaa
    56221 acattctgat ttaagtttag catctgcctc attacagtta aaaatacata taaaaaattc
    56281 ctcaccacca tatcgtatag catgttcatt ttcagaactt gtaaaatcta aaattacttt
    56341 ggcaatattt tgtagacaat agtctccctt ttgatgtcca taattatcat taaattgttt
    56401 aaaataatct atatctatca tcattccagt tataggttct tgatttgcat ggctgattaa
    56461 ttttttttgt agaatatctt caaggtatct acggttgttc attttagtaa ggtcatctgt
    56521 cataactttg tcgttaagta aattatttat atgagatatt tcttcatatt gttgactaat
    56581 taaaagttta tcatagtaag taaatacacg atttctatat agcattgtag aaacaaaaat
    56641 ggcaagggat gttataaaaa atgtgttgac aatattacta aactcattgc ctggtatagt
    56701 ttgaatccca ggtaagaata aattaagaaa caaaaagcat cctccaaaaa ctattactga
    56761 ttctccaaat tttaatactg agaaagctgc agctgctaac ataatataac taaatacact
    56821 taggtcattt ccacctaact ggtcaagtag agtaattaca caaccccata ggcagaaaaa
    56881 agtcacataa attatccata aatttatata aaacgtacag caatcctttt ttttattcca
    56941 aaggtaagaa aatacaaaca taaagacaga agatacacaa aatagtgtta catacatata
    57001 aaaataccat tggcgtcttg gtgtgacaaa agggcctcct tctctagctg agattgatat
    57061 catcataatt atttgagata ttactattat tatgcaaatg attttacccg tatgataaag
    57121 ataattgcag agttctttct caacattgtc tttgtgtttt ttgtctagag gaaatccaaa
    57181 aaattttttt attttttcta agttcatata agcctccatt aaaaataaag cataaaaata
    57241 agtattcctt tctatcatta gatatttgga atacttcgtt tatgatttaa ttatatcaaa
    57301 atatattgtg tgtaacaatc taaaattgtg atagata
//
LOCUS       DAELNY010000005        54252 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.005, whole genome shotgun sequence.
ACCESSION   DAELNY010000005 DAELNY010000000
VERSION     DAELNY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.005"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(271..1266)
                     /locus_tag="KPE55_000260"
     CDS             complement(271..1266)
                     /locus_tag="KPE55_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0491362.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(1550..2386)
                     /locus_tag="KPE55_000261"
     CDS             complement(1550..2386)
                     /locus_tag="KPE55_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG0491363.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(2874..3437)
                     /locus_tag="KPE55_000262"
     CDS             complement(2874..3437)
                     /locus_tag="KPE55_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491364.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(3535..4464)
                     /gene="glsA"
                     /locus_tag="KPE55_000263"
     CDS             complement(3535..4464)
                     /gene="glsA"
                     /locus_tag="KPE55_000263"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG0491365.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(5367..7034)
                     /locus_tag="KPE55_000264"
     CDS             complement(5367..7034)
                     /locus_tag="KPE55_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG0491366.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(7108..7863)
                     /locus_tag="KPE55_000265"
     CDS             complement(7108..7863)
                     /locus_tag="KPE55_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG0491367.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(7873..8409)
                     /gene="lepB"
                     /locus_tag="KPE55_000266"
     CDS             complement(7873..8409)
                     /gene="lepB"
                     /locus_tag="KPE55_000266"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0491368.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(8575..9111)
                     /gene="lepB"
                     /locus_tag="KPE55_000267"
     CDS             complement(8575..9111)
                     /gene="lepB"
                     /locus_tag="KPE55_000267"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0491369.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(9211..10521)
                     /locus_tag="KPE55_000268"
     CDS             complement(9211..10521)
                     /locus_tag="KPE55_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG0491370.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(10600..11316)
                     /locus_tag="KPE55_000269"
     CDS             complement(10600..11316)
                     /locus_tag="KPE55_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG0491371.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            11561..12832
                     /gene="sleC"
                     /locus_tag="KPE55_000270"
     CDS             11561..12832
                     /gene="sleC"
                     /locus_tag="KPE55_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG0491372.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(13309..13965)
                     /locus_tag="KPE55_000271"
     CDS             complement(13309..13965)
                     /locus_tag="KPE55_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491373.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKIMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(15319..15729)
                     /locus_tag="KPE55_000272"
     CDS             complement(15319..15729)
                     /locus_tag="KPE55_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491374.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(15945..17327)
                     /locus_tag="KPE55_000273"
     CDS             complement(15945..17327)
                     /locus_tag="KPE55_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0491375.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(17338..17697)
                     /locus_tag="KPE55_000274"
     CDS             complement(17338..17697)
                     /locus_tag="KPE55_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0491376.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(17973..19163)
                     /locus_tag="KPE55_000275"
     CDS             complement(17973..19163)
                     /locus_tag="KPE55_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0491377.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLFDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTSEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTQIDMQNRILNIDHQLLKDTEIGYYIETPKTKNGKRELPLTE
                     RAYQALQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYVGMLRGLIKKYNKYHK
                     DKLPNITPHSLRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLGC"
     gene            complement(19242..19445)
                     /locus_tag="KPE55_000276"
     CDS             complement(19242..19445)
                     /locus_tag="KPE55_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0491378.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(20053..20292)
                     /locus_tag="KPE55_000277"
     CDS             complement(20053..20292)
                     /locus_tag="KPE55_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0491379.1"
                     /translation="MEKSVKKCPPYHLIASAVDSNEKAIEKLLQFYDPYISKASMRPL
                     FDSYGNVYFAIDMELKGLIREAIMLMIPNFEVEIK"
     gene            complement(20297..20725)
                     /locus_tag="KPE55_000278"
     CDS             complement(20297..20725)
                     /locus_tag="KPE55_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0491380.1"
                     /translation="MKPTTFENAIRLQFDTLVKRVIDTTVKDYDRHMKFLSDHEKSFC
                     ELPEIKVNSFAIQDDYEMDVTVFDVYGMEARVSGDELCKALKRLPEKKRNNLLMFYFL
                     DMSDTEIAELQKISRAGAFKNRQNALNKMRELLQKSETEE"
     gene            complement(21096..21461)
                     /locus_tag="KPE55_000279"
     CDS             complement(21096..21461)
                     /locus_tag="KPE55_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491381.1"
                     /translation="MKKSEEEQKIFLSICSAYDRAVLTGKEEMKKEDFERITHITIAL
                     GFIPYTQLICSQHMDLAVELEEHEEREREIYLEYPEYYDDEEIYEKYDKWIEDFCSQI
                     PEDRQEYYRTLIKENKQEY"
     gene            complement(21478..21702)
                     /locus_tag="KPE55_000280"
     CDS             complement(21478..21702)
                     /locus_tag="KPE55_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491382.1"
                     /translation="MIRYTKRDSKKILKQVARLRGISVSEIREELEIAIEEARNNPAP
                     DTETEFYRLFGNKTPTPEEFLCIATNNFRP"
     gene            21862..22230
                     /locus_tag="KPE55_000281"
     CDS             21862..22230
                     /locus_tag="KPE55_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491383.1"
                     /translation="MELDYKAIGKRIKIARIKKNLTQEAIADKIGITPQHVSNIETGN
                     ASVSLTTLVAIANTLTVSVNDLLCDTVLISKAVFEKEAKELFEDCNEYEIRVLVDVLK
                     ATKQSLRTVKLFEDKIEDNR"
     gene            complement(22268..22675)
                     /locus_tag="KPE55_000282"
     CDS             complement(22268..22675)
                     /locus_tag="KPE55_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBG0491384.1"
                     /translation="MLIVEFKENEEIIFQQTIEWLTSKSFNSSILITQELSEFQINPF
                     TRSVVNKNGELLFLTSKEFDLLYFFYSHKGQVFTKEQLYENVWGFEDMPKDTSNLPSF
                     IRKLRKKIEPDPDNPIYIITVWGVGYKFSEEKP"
     gene            complement(22804..23781)
                     /locus_tag="KPE55_000283"
     CDS             complement(22804..23781)
                     /locus_tag="KPE55_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG0491385.1"
                     /translation="MNKKELHDFIKEKQPNICQISCYKDGKEVYSDEWNDYKKIDTCH
                     VMSATKSIVALLVGIALDKGFIENIDQPVLDFFPEYKTKRGEKTIQQVTIKHLLTMTA
                     PYKYKYEPWTKICSSDDWTVSALDFLGGRKGLTGQFKYSTLGIHILTGIISKTSGIKV
                     VDFANKYLFEPIGVEEHINYLAETAEEHKHFTISKEPKKNIWFCDPKGVGTAGYGLCL
                     SATDMAKIGQLCLDKGVHNGKQIVSSEWIEQMTKTTYKCGEEFRNMSYGYLWWIVDEN
                     KHIYSAIGNSGNVIYVNPSNNIVIAITSYFKPTIFDRIDFVQKYIEPFF"
     gene            complement(23860..24681)
                     /locus_tag="KPE55_000284"
     CDS             complement(23860..24681)
                     /locus_tag="KPE55_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0491386.1"
                     /translation="MLSIGEFSKICKVSTKTLRYYDEIGLIKPSKINQENSYRYYSIE
                     QLETMLFINRLKQYRFSLEEIKAIITSEEILNEKLSIELYKKKIEFEKQIQIYSQIIE
                     RLNEDISVLKQGKSIMSYLNKIDVQLVEVPVMYLVSIRKMVCKVEMEEQYACCFHSIL
                     RKIQYNKLTVNAPPMVLFHSDEFTPLGLDTEFAIPVEQFVTGTRDFCPGLCLKTTLYG
                     GYSNLPSIYTKQCEWAEQNGYENNGPLYEVYITDPTQTSNEDDLVTEIYYPVKKK"
     gene            complement(24864..25268)
                     /locus_tag="KPE55_000285"
     CDS             complement(24864..25268)
                     /locus_tag="KPE55_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBG0491387.1"
                     /translation="MILIEISDNEEKIVQQAIEWIAEQLSTSTISENKSKELQFSLFK
                     RNVIDKNGIAVSLTSKEFDLLYFLYLHKGQVFTKEQLYENVWGFEDMPKDTSNLPSFI
                     RKLRKKIEPDPDNPIYIITVWGVGYKFSEEKP"
     gene            complement(25347..25538)
                     /locus_tag="KPE55_000286"
     CDS             complement(25347..25538)
                     /locus_tag="KPE55_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBG0491388.1"
                     /translation="MNKLYRIRKGKIFFGVCGGLAKCFNVDAKIVRLLFTVTVFVFPK
                     IIIAYIILMIVIPKEVKIR"
     gene            complement(25531..26280)
                     /locus_tag="KPE55_000287"
     CDS             complement(25531..26280)
                     /locus_tag="KPE55_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0491389.1"
                     /translation="MRILLWAEFQKLRRSNIIMFTIFATILIAVIVFVSGVTTVSDNQ
                     LTTSTVGWYMTITQVWATMFVLPAVIALLGSYMICREEQDNTLKSLRLIPVNETKLTT
                     AKMIVALVFSMLVYLLLFVITFMTEVILHFSDLSMVVFVEFLKMYLLEGIGVFFAVSP
                     IIAIVPYLKKSYWLALLLAEIYSFAGLFMSMSNTARTFYPITAIFGISGYYETTIQNY
                     ICSLGILILCGCISAILLKNLNHRQRKKANE"
     gene            complement(26290..27024)
                     /locus_tag="KPE55_000288"
     CDS             complement(26290..27024)
                     /locus_tag="KPE55_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0491390.1"
                     /translation="MLKLVKLEFCKLRRKKLIWLMLLSAFVMPFFAFLYFGYLGQTNI
                     DPRLFYKWSAFGYTLFIILPFILGLFGTMLMHDENRYDMLKQLWIVPVSKMGYFFSKF
                     FVVLIYSVCFMLITAFASVLFSVIPNYVAFDFSSILLLFERCLEIAILTAFAILPILA
                     ISASQKGYIFPICITLLYVFAGFLITPINSCLHPLSCISVIVARNEAILGLALPQNSI
                     SLALLSICVWGIASVLLAKIKLSKRK"
     gene            complement(27017..27940)
                     /locus_tag="KPE55_000289"
     CDS             complement(27017..27940)
                     /locus_tag="KPE55_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0491391.1"
                     /translation="MSDTVIKTKQLTKKYGKQTVVNKVNLNVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTTTTSGNVEVFGKNIDGNEKRIYSRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTASPNAVKHALEVVGLPYKDKKTFAKYSLGMKQRLGIANAILHDPELLILDEP
                     TNGLDPIGIAEVRDFIKKLSVEHGKTILISSHILSEIAVLADDIGIIDKGVLLEESSM
                     SELEKRNRKYISLQVSDIPKTILILERQFHVKDYSVQDEQTVQIYDTTLNMGEVNKAL
                     VMEDITVISSGLCNDTLEDYFKKITGGEGIA"
     gene            complement(28092..29009)
                     /locus_tag="KPE55_000290"
     CDS             complement(28092..29009)
                     /locus_tag="KPE55_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0491392.1"
                     /translation="MNTYLLIAFVLALVIIACLVYKLYHINEQISFIKEVLVDIKSGN
                     LNRRILVQENDITKQICYDINQIAINNQSQLIHQKQSEQAYKRLMTSLSHDVKTPLAS
                     LVGYLEAVESQIVIGKEKDEYIHVASEKAQHLKHFVEHLFEWVKLDSGEQIFHFENLD
                     LNELTRNIMTDWILILENSHFEYEVEIPEEEYQVRIDINAYTRIINNLLQNIIVHSEG
                     NKMMLRVLKDQQRIKVIITDNGKGIASDKLPHIFERLYQCDNSRSTKGNGLGLAITKE
                     LVNAHKGTITANSILNHGTEFILSFPKAL"
     gene            complement(29012..29710)
                     /locus_tag="KPE55_000291"
     CDS             complement(29012..29710)
                     /locus_tag="KPE55_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491393.1"
                     /translation="MGSVLIIDDDKELCTLMKKCIEQEEMSALVANGGTEGLRLIDEN
                     KNLFSLVILDVMMPDIDGFQVLEKIRETSNTPVLMLTAKSGEDDKVFGLRLGADDYLT
                     KPFSIKELMARVNSLIRRYTTLNPTFAEEDCITLKGMKIDKVNRTVLVNNISVELTSK
                     EFDLLTFLASNKGRIFTKKQIYTQVWKDDYAFDDNNIMSFISKLRKKIEADPEHPFYI
                     LTIRGVGYRFNKEA"
     gene            complement(29772..29996)
                     /locus_tag="KPE55_000292"
     CDS             complement(29772..29996)
                     /locus_tag="KPE55_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491394.1"
                     /translation="MIGLNKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQANFRKYFGNKRPTPEEYIYKITKKTKV"
     gene            complement(30012..30341)
                     /locus_tag="KPE55_000293"
     CDS             complement(30012..30341)
                     /locus_tag="KPE55_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG0491395.1"
                     /translation="MELEELIVEIVIGLFLLFTSYQIGIKENITLLHGYHYTQLDPKD
                     KKVFTKKIGIGSLLVSIGILVMPIINLISHSELGYYMGLTLIVVGVFYIIFIIVKYNG
                     KLISFKK"
     gene            complement(30356..30565)
                     /locus_tag="KPE55_000294"
     CDS             complement(30356..30565)
                     /locus_tag="KPE55_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491396.1"
                     /translation="MDEHLILRNRLKTVRKEKKLSQTELAELVGVSRNTISSIETGQF
                     NPTAKLALVLCIALDKKFEELFYFD"
     gene            complement(30567..30881)
                     /locus_tag="KPE55_000295"
     CDS             complement(30567..30881)
                     /locus_tag="KPE55_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491397.1"
                     /translation="MDKEKILSQNKKDNLYLDEYEKHIKLQGKSFGLMFVLFICILIL
                     FIKAVCKEPYYDIMTIIGSVAFGFMGYEAHISKNKSKFVIALFFLLFMGYYFYKFLMV
                     GL"
     gene            complement(31026..31928)
                     /locus_tag="KPE55_000296"
     CDS             complement(31026..31928)
                     /locus_tag="KPE55_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0491398.1"
                     /translation="MFKKNKKSTENIKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEVRTQAISG
                     YLTKELQDLNVDTIRTDIPTSSTVTDVIVWSIEQSGTDTFSATYEVDQQIKEGEQISN
                     VKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTAEADASVDADTVNDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGDNVKVKVAVKFLDN
                     QTKATQVSQYELVLHKDNNWKIVG"
     gene            complement(31948..32952)
                     /locus_tag="KPE55_000297"
     CDS             complement(31948..32952)
                     /locus_tag="KPE55_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG0491399.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVEYVAGKGKKHTYNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYQYGNQFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGILVSPTQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     "
     gene            complement(32949..35099)
                     /locus_tag="KPE55_000298"
     CDS             complement(32949..35099)
                     /locus_tag="KPE55_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0491400.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSYGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNVEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNMASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRMEKQEQRKKNIADKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PTTAPASKASEPVKTNVVKERPLSSGASDKKATQPAQPVHRQNVEKVVSQETRQNDTK
                     DRRTKVQQTQSVQKNQQTTEKTRNLVTKKGQKKK"
     gene            complement(35099..37549)
                     /locus_tag="KPE55_000299"
     CDS             complement(35099..37549)
                     /locus_tag="KPE55_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491401.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSQQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCMIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPVSRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(37527..37925)
                     /locus_tag="KPE55_000300"
     CDS             complement(37527..37925)
                     /locus_tag="KPE55_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0491402.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            complement(38034..38537)
                     /locus_tag="KPE55_000301"
     CDS             complement(38034..38537)
                     /locus_tag="KPE55_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0491403.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            complement(38555..39058)
                     /locus_tag="KPE55_000302"
     CDS             complement(38555..39058)
                     /locus_tag="KPE55_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0491404.1"
                     /translation="MQETRVLVETKGTTGEETTSYWFDLPIDVAEFEEKLGIGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFSVTEH
                     GIFELPE"
     gene            complement(38977..39273)
                     /locus_tag="KPE55_000303"
     CDS             complement(38977..39273)
                     /locus_tag="KPE55_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0491405.1"
                     /translation="MSDIFKDMQAKVGCDYLSDLPSYKRKVWHEMKRLNLADYEERQL
                     EDFSKYVFGMSYQTIKDVMKQQKGREEQCRKQGCWWKRKEQLAKKQHHTGSTCR"
     gene            complement(39361..39723)
                     /locus_tag="KPE55_000304"
     CDS             complement(39361..39723)
                     /locus_tag="KPE55_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491406.1"
                     /translation="MNFNEKLINLRKSKGLSQEELGNELKVSRQTISKWESCQSYPDF
                     QRLVLLSDFFGLTLDELVRDIDVQEVREKNLNNEKLSSIYDDINEGKKFIRLCYIIGT
                     GVLIFFSLIVLYGVFFSK"
     gene            complement(40073..40699)
                     /locus_tag="KPE55_000305"
     CDS             complement(40073..40699)
                     /locus_tag="KPE55_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0491407.1"
                     /translation="MKYIMLHGLGQSSESWSDTVKVFNDDFEVLCPNLSDWLSGKTPC
                     YDTLYRTLETYCEQFEEPLNICGLSLGGILAMQYAIEHPEKMNSLVLIGTQYEMPKYL
                     LKMQNLIFRIMPCSAFEKMGFQKTDFINLCKSMTELNFKHELKKISCSVLVVCGDKDK
                     INKKASLELQQQIENAEIAIIENAGHEVNTDNPTLLGQKLNVFFKQYV"
     gene            complement(40900..41370)
                     /locus_tag="KPE55_000306"
     CDS             complement(40900..41370)
                     /locus_tag="KPE55_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0491408.1"
                     /translation="MMNYSIRELDAFSVIGQEVELTNYQKRNIQISTQFWRKFNSNLK
                     KSYLSQSGNWVKYAFMERRNGKLYYFCSIPKRTIIPDNFLYKEIPSYKYLVMEHIGAM
                     EKIYETYGKIYQEIIPNTPYTPIKNIILHFEKYDYRFHWNRDNSVIEIWIPIQD"
     gene            complement(41367..41609)
                     /locus_tag="KPE55_000307"
     CDS             complement(41367..41609)
                     /locus_tag="KPE55_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG0491409.1"
                     /translation="MANELLENLDRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEIIRKGKNWYITIDNCIITINAYSYTIITAHKVK"
     gene            complement(41696..41917)
                     /locus_tag="KPE55_000308"
     CDS             complement(41696..41917)
                     /locus_tag="KPE55_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491410.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(41918..42052)
                     /locus_tag="KPE55_000309"
     CDS             complement(41918..42052)
                     /locus_tag="KPE55_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG0491411.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCMLSVGKEADYKMKQLRESEDN
                     "
     gene            complement(42065..43261)
                     /locus_tag="KPE55_000310"
     CDS             complement(42065..43261)
                     /locus_tag="KPE55_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0491412.1"
                     /translation="MVLNEEQWIKELREKRVAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(43506..43922)
                     /locus_tag="KPE55_000311"
     CDS             complement(43506..43922)
                     /locus_tag="KPE55_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491413.1"
                     /translation="MEIKEINKIIQSEDKDEKSKSVKCICINYGDFLADCEGFVQKRY
                     HDFKCNPKHQFEKKADTILENAIHEKNFMPDLFLIRLNRKQSACNSQIDFVFELLDKS
                     FLETDPIRKSEISEETLNLCLSADVSFIMVYIGMNR"
     gene            complement(43926..45308)
                     /locus_tag="KPE55_000312"
     CDS             complement(43926..45308)
                     /locus_tag="KPE55_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491414.1"
                     /translation="MRKIWNKGHRIRASDKHLVYHFFIGSLLFVFVAVLFLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     VLENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGRLAQERQ
                     VMPEQQGKEN"
     gene            complement(45380..46267)
                     /locus_tag="KPE55_000313"
     CDS             complement(45380..46267)
                     /locus_tag="KPE55_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0491415.1"
                     /translation="MLWLWLFISGLILVIVLLLIKIHLLRKSAKEIECSFAEKLITDS
                     NTLIDISSNDKYMCHLANEINIQLRKLRAERRKFQKGDIELKNAVTNVSHDLRTPLTA
                     IYGYLDLLEQEEKSEKVNRYIAVIKNRTEMLKQLTEELFRYSVILTSEEDLKIEPITI
                     NNVLEESIASFYTILKEKQISPKIIIPQKKIVRNLDRSALSRIFANLLNNVVKYSNGD
                     LEVTLFENGEIHFINHASNLTEIEVGKLFDRFYTVNNARNSTGLGLSISKALVEKMNG
                     TISAEYNNEMLNICIVFDL"
     gene            complement(46282..47109)
                     /locus_tag="KPE55_000314"
     CDS             complement(46282..47109)
                     /locus_tag="KPE55_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0491416.1"
                     /translation="MNKLLSANFFRLRKNKCFRGSLAFMFVIGIASPLLRYRDMKQSG
                     YINNLDNGFFMCALFIGIILAVFCSLFIGTEHNDGTIRNKIIVGQKRETIYLSNMVTC
                     SIIAITMCIVFFVPYLSVGIPLLGFFVADMKMIVMIGITVLVLSVTFASLFTLVAMLS
                     QNKAIIAVACILFSFGLLFAGAMCNRMLDAPKTIPAYSIGENGENTAQEMENPKYLDG
                     TKREIVQFIYDVNPGGQAIQCSTMQVVNLTRLPIYSLVIVILTTGAGVWIFKKKDLK"
     gene            complement(47102..48016)
                     /locus_tag="KPE55_000315"
     CDS             complement(47102..48016)
                     /locus_tag="KPE55_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0491417.1"
                     /translation="MEYVLKTNSLCKNYGHFKALNGLSMNVPKGSIYGLIGKNGAGKT
                     TLIRLICGLQEPTSGDYVLYGKKHIDKDITKSRRRMGAIVETPSIYVDLTAYENLKEQ
                     YKVLGIPSDDGISEILKLVGLENTGNKKAKNFSLGMRQRLGIAVALAGSPDFLVLDEP
                     VNGLDPQGIIEIRELILKLNQEHGITVLISSHILDELSKLATYYGFIDNGKMIKQISS
                     IELESKCRKCTHLKVSSTKTLAYVLDTMNIEYKILSNTEADIFEKVNISKLTTELSKK
                     DCEVISIHEHDESLESYFVSLVGGARHE"
     gene            complement(48106..48768)
                     /locus_tag="KPE55_000316"
     CDS             complement(48106..48768)
                     /locus_tag="KPE55_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491418.1"
                     /translation="MKHILIVDDDIHINKMLDEVLVHERYMVSHAYSGTEALLFLADT
                     KPDLILLDLMLPGLSGEELLPQISDIPIIVMSAKVDVEDKVSLLLGGAVDYVTKPFDT
                     KELLARITVALRVKTPSLNRTLEFEDITLDTFTYEVFIEDKKVKLTRTEYAILKLLIQ
                     NPKQVIVKSFLLEKISEDTPDCTGSSLKMHISNLRKKLRDISGKDYIEAVWGIGFKMR
                     TE"
     gene            complement(48851..49234)
                     /locus_tag="KPE55_000317"
     CDS             complement(48851..49234)
                     /locus_tag="KPE55_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0491419.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNPTPQGQQKK"
     gene            complement(49250..49576)
                     /locus_tag="KPE55_000318"
     CDS             complement(49250..49576)
                     /locus_tag="KPE55_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0491420.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESDHVFDRDKDNRRFFARRSYNL
                     YSDVQRGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLLADDI
                     VAVEEK"
     gene            complement(49815..52859)
                     /locus_tag="KPE55_000319"
     CDS             complement(49815..52859)
                     /locus_tag="KPE55_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0491421.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFRNGYKTRADASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKASSNTSITDGNG
                     NYSVAGAIYGVFSDKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKIEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYTKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVFPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPDGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLANVPFRITSKTTGENHVVVTDDNGQFSTSAEWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADEEEMKVEISYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKVEVIFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKTETPPTPEKPS
                     RPSDSPKTGDNTNLYELLVLLLTSGAGLAGIFFYKRRKMKKS"
     gene            complement(53255..54034)
                     /locus_tag="KPE55_000320"
     CDS             complement(53255..54034)
                     /locus_tag="KPE55_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG0491422.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT        16500 a        10884 c         7656 g        19212 t
ORIGIN      
        1 tttttgaaaa ataaagattt tatgtgttat tcgacattat attgatactc cagtatttga
       61 aactatacta atacaacaat aataattgtg ttatgtctat actcaaacaa atgtttttaa
      121 aaagcattaa gtaaatattt aataagtatt aaacaaatat taaatttata taatagctat
      181 aactatgtaa atttaatatt tgtatataag accacatcta ataaaaagaa atcggagtaa
      241 atttaataca ccgatttcaa taaataaaat ctatcctagc ttttcaacaa cagtcttaag
      301 catttctctt ataggaagtt catatggaca tctactctcg caaagcccac agtcaataca
      361 ctcagtaggt ttaacatcca taacctcata tttttcttta gcccactctt ttaatccata
      421 tctagtataa taaccttcac ataaaaaact caatggtata tttattccta cagcacaagg
      481 catacaatat tcgcatcttc tacagaattt tttacctaaa gaatttctta tttcttcaat
      541 ttccttatta tctttttcat ctagcacaag attttcaagt acagcgacat tttgtctaac
      601 ctgttcaaca ctctccatac ctggtataac tacatctatg tactctttag atagtatata
      661 tttaatagcc agtgttgcat tgtctaaagc acctccagct aaaggtttca ttactattat
      721 cccaattcct ttttcatgag cttttttaaa cacttcatct gcttgtcctt caactatatt
      781 gtaagggaat tgtatagtgt caaattttcc atcttctata gctttttcta tagtatttaa
      841 attgtgacta gttataccaa tatgttttat cttaccttgc tcctttgcct ctaaaagggc
      901 actataagcc attttatctt taaaaagatt atcatactcc tcttctttaa cattatggaa
      961 ttgatataaa tctataaaat cagttttaaa attatttaaa cttatttcaa catctctctt
     1021 cattgaatca taatctctgg acatactttt agtagctaag aaaaatttat ctctttttcc
     1081 ttcaatggca attccgattg cttcctcact tattgtatac cctcttgcac tgtcaataaa
     1141 gtttattcct tgtttttcta actcatttat aactaagtta gtatcatctt gagtaatcct
     1201 ttgtattgga atgcccccaa agccaactct ctttattttc atgttagtat tgcctaaatt
     1261 tctcatatct ttccccacct aacttttaat aattttttat ataatataaa tatgtcatct
     1321 ttaattatac aacaaattat gagaatcttt gcaatatatt tacatactat tcataatata
     1381 attatatttt atatcagtct attcataata taatcatact ttatacccag ttaatttgta
     1441 tacaattttt gttattccta tccattaatt taaattatca catttcatga ttatatctcc
     1501 accctaatta tgttgagggc ggagtatttc acatattttt aataaaggtt tatttactag
     1561 atagtatttc ttttatcatt ttaagctctc tcttgtgacc ttcatcatca aaaggagttt
     1621 ctaagaaaaa tggtaaatgt tttagactag gatgttccat aaaatcaatt aatgctttta
     1681 atcctatctt tccttctcct ataggagcat gtctgtcttt tttgtctcca aatggcatca
     1741 tactatcatt tagatggaca gttttcaatc tttctatgcc tataacctta tcaaattctt
     1801 ccaaaacccc atctaaatca ttcactatat cataaccagc tgaaaaaata tgacatgtat
     1861 caagacacac acctattttt tctttatgct caattttgtc tattatactt tttaattgct
     1921 caaatttaaa acctatttca gttccctttc ccgacatagt ttcaagaagt atagttatat
     1981 tttcatcacc ctttatggca ttgttcaatc cttttactat tctatctata ccaaaatcta
     2041 caccaccacc tacatgactt ccaggatgaa aacacatgta ttcaattcct aatgagtcca
     2101 tccttcttat atcctcatct atcacccttg ttgcaaattc gtatacatca tcctttgtac
     2161 cacccagatt catagtatat ggtgcatgtg caagcagtgg tgcaaaatta ttttcttttc
     2221 taatttcttg aaatttaatt atatcttttt catcaaattc tttagcattt cctcctcttg
     2281 gatttctact aaaaaattga aatgtatttg caccaatata tacagctgtc tcagccgcat
     2341 tagcaaatcc ttttgctatt gaaagatgtg ttcctaattt aatcatatta atctcctttt
     2401 atttgtacta cgacttgtac tcaattttaa ttttgaacta ctccacctta actacattta
     2461 gccaacatat gaagatggag attcctattt gatagtactt tttcactaaa aattcataag
     2521 ctctaaggga tgttctatcc caactaatct cattcctctt ttaacatatt tctagcattg
     2581 atttaattta tattaatttt taattttaat atttacactt taattatacc cattttaatc
     2641 aatttcaaat attataatct tacatacttt gtatattatc taagcacttc ttagatagtg
     2701 tatacatctt acactataca cattatttac aaatacaata ggtataagaa aacaaaaaaa
     2761 tagagattaa aaatcattgt tttacccata ccaaaaacta ttttttgatt attaagtaaa
     2821 ttttgatgtt caatctctaa tattatatct ttaattaatc cataattaat tttctaacta
     2881 ggacattcct cctcaagagt aggaactata accatagcta tatcatactt agaaccttca
     2941 acattgtgtt gtcttttatt taaaatttca tctatctttg acttcatagt gtcactaagt
     3001 tcaactaatt catcatgagt aagagaaata tcatgataat caactatatt ttcattttct
     3061 aaaacatctt cctctgctgc cttataaaaa acttcactca tattttcaaa ttttgatatg
     3121 tacaattctt ctttttcttc cccattgtct aaagaaaaat taagtatcct attccctata
     3181 accacatttt ttgcacttgg ataatagtac ttttccacaa tccccgactt tattttttcc
     3241 tcaactaatt ctaaaatacc aactttgtat aacattttta tatggtagtt tatttttgca
     3301 tgaggttctt ccaataattg agataattgt tttgctgaca atggcgattt gtcaaatgct
     3361 tttagaatat ctattctacg tggatgcgca atagccttta tgcactccaa atctcgtaat
     3421 accaatacct ctttcattga cccctcctaa aaaatttatt taattcatat ttctttacaa
     3481 tttaattata tattaaatta tatgttgttg tcaaaaaaat cctttcttaa tattttaaaa
     3541 aatatttaat ttgagtttat ttgacaactc ctctaaaact ttaactcctg cgacgctgtt
     3601 accttttttg tcaagcgcag gtccataaac accaatcccc atgcgtcccg gcacagaagc
     3661 cattatacca cccccaactc cagattttgc aggaatgcca actttaatag caaattcccc
     3721 agaagcatca tacattccac aagtcatcat aaatgtcttg acaatcctag aaaccatttc
     3781 actcattaaa tgctcaccat tttctatatc tactccataa tttgcaagat ttattccaat
     3841 tctcgctaaa tctacagaat ctatctcaat tgagcattgt ttaaaataaa ggtctagaac
     3901 atcttctaca ttccctctga taaatccatc acttttcaat aagtaagcca tagctctgtt
     3961 tctatctcca gtcatttttt cagacttata gacatcatag tttattccta tattatcatt
     4021 ttttgctaac tttctaaaga aactaagcat tctttcttct ttttcttcta aacaactccc
     4081 atttataagg gaagtggtta ctatagcccc tgcatttatc attggattac agggtttttt
     4141 tgtgtcatta atttcaagtt tcattattga attaaatggg tccccacttg gctccattcc
     4201 aacgtttgaa aaaacgtctt cccaatcatt atccataagt gccatagcaa gcacaattgg
     4261 ttttgaaata ctttgtatag taaactttgt gctacagttt ccagcactat aaagattatt
     4321 ttcgctatct atgatacaaa ttcctaaatc atttcttcta gcgtttttta attctggtat
     4381 gtaactagcg acttgtccat aattagtata cttcttatta gaacttataa tttcttttaa
     4441 taatgtctca tctaacgtat tcatatgcac ctcttatatt atgttatata ttctatttta
     4501 gcacaagttt atactataat aaaacaatgt taaaaaaaat tatactttaa aattattttc
     4561 tacaagaatc aattcatttt tcaaacaatt taaatagttt aaagagctta ataatttagt
     4621 aattcaataa tttaacaacc tagcaattta gtaatctaat aatccagtaa tttaacaacc
     4681 taataattta gcaatctaat aattcaataa tttaacaacc tagtaatcta actatctaat
     4741 aatccagtaa cttaacaacc tagtaattta gtaattcaac aatctaataa ttcaataatt
     4801 taacaaccta gtaatctaac tatctaaata atccagtaac ttaacaacct agtaatttag
     4861 taattcaaca acctaataat tcaataattt aacaacctag taatctaact atctaaataa
     4921 tccagtaact taacaaccta gtaatttagt aattcaacaa cctaataatt caataattta
     4981 acaacctagt aatttagcaa tccagcaatc taataatcca gtaatttaaa tttttataac
     5041 accaaaaatt tctaacccaa caccttttac tcttttactc ttttactctt ttactctttt
     5101 actcttttac tcttttactc ttttactctt ttactctttt aaaaaataat cacataaact
     5161 aacaaaatat taaacaaaaa agtaaaattt aaaataaaaa gcttgcttca tacaaaagta
     5221 taaaacaagc ctttttatat atcaactcaa actttaaaat ctgtatcaat ttaattctcc
     5281 actttacact taatatactt aacataaacc aatcacacta cttgctatgc catttaatct
     5341 tcataaaaca atctataata cacacattaa tcaactattt tacttcctcc aataaattcc
     5401 ctaagaattg aaatgcctgg tatgtcagaa gtatcgtcca attcaacaaa atactgcaaa
     5461 aactttagta atctagtagc ttcaatatgt gcaggattat cttcctttat ttcttccaac
     5521 aaaggtttcg caaatcgttt taaaactgcc aattcatata cacaagcaga gttaaatata
     5581 gcatctgcct cctcttgata cgggaatata ttcttctttt ctcctcttct aactgatgcc
     5641 cattgtaaaa tagtattatc agcactatat cctctaaaat tatagtctct aacaattctt
     5701 cttatcattc tcaaatcagc agtagggatt cgattatggt catccaaatt tatttgagta
     5761 agggcactta tgtatatctt aaatttatct tcatctggta tagatgatgt taaaatagga
     5821 tttagcccat gtataccttc taatataatt ggctgatttg aatctatctt tatcttttta
     5881 ccagtgtatt ctctcttacc ttctttaaaa ttgaatctta tatcatcaat ttcttcacca
     5941 gctaaaagtt tctttaagtc taagttaaac ttctctaaat ctattgcata aattgattca
     6001 aaatcataat ttccatattc atctaaagga gtatgttttc tatctataaa ataatcatct
     6061 aaagaaattg aaataggatt taaattattt actctaagat gtattgataa tctgtgagca
     6121 aaacttgttt ttccagatga agatggggct gctattaaaa ctactctctt attgccatcc
     6181 tttatcatat ccgctatttg ggataacttc ttttcatgca atgcttcaaa tgtccttatt
     6241 atatctccat attcattgtt ttctattatt ttattaagag atactacctt gtcaattccc
     6301 ataagtcttg accattcttc tgcttcataa tatacacttg atagcttagg ttgaggtaca
     6361 aatttcatag gaagagtctt atcttcttca ctaggtccta aaattataac gcctccatta
     6421 taaagcttaa tatcaaatga ttttatataa ccagttgatg gcaacatata accataaaaa
     6481 tggtctatat agtttctaca cttatatact ttagcactat catactcttt gtatttcaaa
     6541 agctctgcct tttcatacat ttcattattt tcaaagatag aaatagcttc atttcttgta
     6601 acttcaattt tttctataac ataatcgttg tttactatct ctttcatctt gttttttata
     6661 ttttcaacat ctgcttcttt aagctttcta tctatatgaa cttcacaata tagcccatct
     6721 gatagtgaat gttctattgt aactctgcta tcccaaaaga tttctctaca tgccattaca
     6781 aatataaatg acatgactct aaagtaaact ctctctccat cttcattagt agtatctaaa
     6841 aaatttatct cacaatcctt cttaactcta caatttagct cttttagttt attgtctaca
     6901 actgcaagtg ttatataacc cttatgctca tcacaaaatt cttttgctat atctcccaat
     6961 ctgattccag gggaattttc ttctaatgaa tattccttat cttttccttc tatttttaag
     7021 tttatttttt tcacttcaac atctccttcc ttttgatgaa tataaatttt aattaatgag
     7081 gcatatcaac aagataaaga gaggtattta accctctcct acaatattat tcaatttact
     7141 tatagtatct tcaatgtctt cgtaatttat ttctaatact aaatattctg catctaatgt
     7201 taagacctct ttaatcccct cttcagataa aattcctctt cctataccaa catgtgtatc
     7261 attgatacca tcattatcac ttaagtgtat cagtcttatt ttatccttta agttctttac
     7321 ataagcatcc tcatcaatta aacagtgtcc agtatcgtaa caaaaacaaa tcttagaatt
     7381 tttaatcctc ttaaatatat attcaaaatc ataagatata tttccaatat ttgaaaaatc
     7441 cccacctttt gaataagtat tctctataca tacatatgaa tttgtattta atttatctaa
     7501 aaaatccaca ctatttccta aatatttgtc tctattcttt ctaactctat ttgtcataac
     7561 atatcccaaa tgaaggttaa aataccttaa atcaaatcct tttagttcaa attctatctt
     7621 ctcaataaaa tttatccatg aatttcgtat atactctatg ttttcgcaag tatttaattc
     7681 cataggcaaa tgtatcccta tagataatcc taacttagaa attctctctt tatatttaca
     7741 taactcacta cattcagata tattgtcaat tccaatctct aaatgagtta tttgctttat
     7801 actctcacaa atgtttaaag cctcctcaat attaaacaat aaggacgcta taccaactgc
     7861 catacaaatc acctactcaa aaatacctat atctgtaaaa ggaaatactc ttaaaataac
     7921 ttttccttta atattttctt catctactaa tcccacttct ttatatctac tgtctaaact
     7981 aacttctcta ttgtctccca tcgcaaatac ttttccctta ggaatcacca tatcaatatc
     8041 gccttcagta taattatcat gtatatatga aacttcattt aataacttac catttatata
     8101 aaccttagaa tccttaattt ttagatggtc accaggaaca cctataaccc tttttacaag
     8161 ctcttttttc cttccattct cttgtaataa atcagtctta aatatcatta tatctccaca
     8221 ttttggttct gataacttat atgtcatcct gtttgctacc aaataatcat gtgatttaag
     8281 tgttgggtac attgattctc catttactaa tgttggtttt ataaaacgag ttataataaa
     8341 tgcaaaaaaa agtgcagtaa taattatttt tatccactct acaatctctt ctttaatagt
     8401 ttcattcatt cacaaattct cctttatagt tatattatct ttattgataa tgtctcttaa
     8461 acactatctt ttagataagt ctactaaata aggtgttgtc ttttttatat agacaactat
     8521 tgtgtatggt ctggtgattt cagctaatca gcgattgctc caaattttat atgtttagtc
     8581 aatagttcct atcttagaaa atggatacaa tctgactaga acctttccta aaatggtatg
     8641 ctcgtcgacc aatccaacct catcgtatct actatctaaa cttttttctc tattatctcc
     8701 cattgcaaat aattttcctt ctggaactac gatatcaata tctccatcag tacgattatt
     8761 atgtatatag gaagtctcat ctaacaactt atcatttaca tataccttgg agtcttgtat
     8821 ttttacatgg tcaccaggaa ccccgataac tctttttact aaatcttttt tctttccatt
     8881 ttcctctgtt aagtcggttt tgaatactat tatatctttg tattttggct ctcctacctt
     8941 gtacgcaatt ctgttaacta tcaaataatc acgttcaact aatgtaggat acattgattc
     9001 tcctttgact attgttggca ctataaaacg agttattgca aatgccaaaa caagagctat
     9061 gacaatcact tttatccatt ctacaacttc ttttttaact gcttcaccca ctaaaatcgt
     9121 ctcctctgtg ttaaatatta agtcaatcta cagcaaaata atctaccata aacctacaat
     9181 ataaatatta tacttctttt ttctaatagt ttaaaatact tttctaagac ctttaggata
     9241 atgattctta attacaccag aagattcttt tccccaacct aaaggtattc cttcaaccga
     9301 aaccaaaacc catcctctac tatcattaga atttacaaca tcccctttta aatatttaag
     9361 aattgattca ctctgtacac tgaagttttc aacatttttt acatcttcaa cttttaagta
     9421 atgagatagt gcatgagaag gctcaaatct attcttcttt aaaaccccta aatgcaaacc
     9481 atatctcaat acttttagtt ttttcgtatc tggatatatt tcaggaagta ggtacaaatt
     9541 ttctcctctt aaataaaatt tatttttttt attactataa aaatcaacct ttaaaaattt
     9601 cttttcaaac tccctataat cctttatttc tttatctaaa ttttttattt ttaattcttt
     9661 gacattacat tcttcttctt ctaatttttt tattttggca acaaaatgac cttctccctt
     9721 tatcttatga ggccataatc tttccatttc aataagttct gcatgtggat attcactgat
     9781 aaactcattt ataacatgct cattttcctc ccttgaaaaa gtacaagttg agtaaattaa
     9841 aatgccatcc ttctttaaca ttttatatcc atctcttata atctcttttt ggatggattg
     9901 acattctact acctttgcat aactccaatc tgatatggca acctcatcct tcctaaacat
     9961 accttgccca gaacaaggtg catctataat aaccttatca aaatacccat taaacgtctt
    10021 tcttaagcta ttagaatcgg tgtttgttat aatacaattt ctcgctccaa atcgctctaa
    10081 attttcccct agagccttaa ttctactagc attaatttca ttagaaacca ataaacctgt
    10141 atcgtttagc ttagatagta tatatgtaga ttttccacca ggagcagcac acatatcgag
    10201 aactttttca ccctctttaa tatctgcctt tggtattaca ctcattgcag aaggctcttg
    10261 caaatagtat gctccagcct catgcagtgg atttctacca ggtctgtcaa ttttatcatc
    10321 ataataaaaa ccttcttctg cccatggtat ttgattcaaa tcaaacaact ccaaatcaaa
    10381 taattttttc ttgtcaattt ttaaagtatt tattctaagc ccagtagtct tactttcctc
    10441 ataacttttt ataaaatcat catattcatc ttttagtatt tcttcaatat cttctaaaaa
    10501 ttcctttggt aattctgtca aatctataat cccccttcaa tctgctttac ttgtgtgtca
    10561 tttattatac tttaattatt tatgtctttc ctttaaattt tatttcttct tctttttcct
    10621 aagattaccg aaaaacactt ttttagagtg attcatatat ttctcaagtt tatcaagatt
    10681 tatcttccac aaaattatta cataaactat aagtaatata atagcccatc tatattctat
    10741 catcaatatg aaatcaaaaa ctccatgtaa taaaattgga attaacactg ccataaacaa
    10801 atactcacgt cttttatttt tatttccctc aaatttatac ttagaaatat agtagcccat
    10861 agtaattgca aacattacat gagctggtat agagataact gccctattaa taccaacttg
    10921 cagtgctaga tttctacttt cagaaaaaat ataaaccata ttttcaattg ttgcaaatcc
    10981 taaagctaaa aatacagagt aaataatacc atctaacttt tcagtaaagt gtttttcttt
    11041 aattagggca ggaatcagta tcagagcttt tacaccttct tctacaagtc ctgcaactac
    11101 aaatgataca tatattagtt ctgaataccc ttcaaataca ttaaacttca tcagtaaatt
    11161 ttctaaaagt ataattataa cacttagaaa agcacctatt aaaaaaaatt taattagatg
    11221 gattaggggt tctctacaat atctatcttt agaccttatc caaaacattc ctatcaatat
    11281 aggaactatt gctaataaaa ataaatctag cttcatagat tattttcctt tcaaaatttt
    11341 tgatttattt atgatttata tcatctactt atttttaact tattcgtgtt ttaaatacat
    11401 actaaaatat acattaaatt attttaaatt atttaaatac aaatatactc aaatactcaa
    11461 aaaaatttaa atttatatct atattattta acaaatttta agctatattc atatcttgaa
    11521 atataaggga actaaattca tttaaagaaa gggtgatttg atgcaagatg gtttcttaac
    11581 agtaagcata attgatgcta ctaacaatag acctattcaa aatgcagtag taaacatata
    11641 ttcaatgtct aatggtagcc aaagctcatc aactttatac caaaacctaa gaagtaatga
    11701 atctggtcaa gtgactggtt tagtattacc agcaccagat gtagattact ctctacaacc
    11761 atctgatgtt agaccttata gccagtatat agtggaagcc atagcagatg gatatgagac
    11821 agttgtcata gaaggaacac agttgttagc tacaattgaa gcaagacaag gagttcccat
    11881 gtctcctaga acaagaagta agcgttcttt ctcaagacaa agtgagttga tttttgacat
    11941 aggagaacat actctatatg gtacttatcc tcctaagatt ccagaaagta acttaaaacc
    12001 attaccacct cctactggtt tcgttgtatt agataaccct gtagtgcctg aatttatagt
    12061 agttcatgat ggtttgccag aagattcatc agctccaaac tattggattc catttaaaga
    12121 gtacattaaa aacatagcat catctgaaat atattcaact tggcctgaac agacaattta
    12181 tgcaaatgta atagctataa tatcatttac tttaaataga gtctttactg aatggtatag
    12241 aaataaagga tataacttta caataacttc aacaacagca tacgaccata agtttataaa
    12301 taatagaaac ttatttgaac caattaacgt agttgtagat gctattttta atacttttat
    12361 aaaaagacct cctacatcaa gacagccact attagcacaa tattgtgatg gacaaaaatc
    12421 ccaatgtcct gaccaaatga ctcaatgggg cagtaaagac ttaggtgacc aaggatatga
    12481 ctatgagagt atactaagat atttctatgg agatgagata gtatttgaaa gagcgcctat
    12541 agtaagtggt gtaccagtgt cattccctgg tactacatta caagttggtt cttctgggca
    12601 gtatgttaga accatacaaa atcagttaaa tgctatatct aattcttatc cagctgtacc
    12661 aaaagttata gaagatggta tctatggaac agatacagaa aacgctgtta agatattcca
    12721 aggaatcttt ggtttacctc aaagcggagt agtagatttt aaaacatggt atgaaatatc
    12781 cagagtttat gtagctacaa ctagaatagc ttctttaaat cctttaattt aaagcttgat
    12841 ttagaacata aactcaaatt attgaataaa attattaatt taaaataaaa atggctgtta
    12901 tgaactaata tataccataa gtattactta taagtattgt agtcttaaca gccatttttt
    12961 agttataact tgttttttta gaatcttaaa aacagtgctt agcaacttag atttctttaa
    13021 aatattatat ttatttttaa gtttaataaa atgctattct aacgaaaaac ctacttatta
    13081 tttaaaatct cacttattat ttaaaatgat acttctcatt tagagtatta ctccttatta
    13141 tttaaaacac tatctatcac ttagagtatt acttcttatt atttaaaaca ctatccatca
    13201 cttagaatat tacttcttat ataaattatt atttattata aatacaaata atattaattt
    13261 aaaaagttat tcctaattta aataatacta ttatcttcat cagtgaacct aaactgcaac
    13321 tttagactta ttagctaaag ccaatattag acctaaagct atagatgcaa atgcagaagc
    13381 aattactcta tactcagcag ggaataaaaa tacaattgta tgacatggta tccagaaaaa
    13441 tggtatagtt tttaaaacac taaaaccaag gtaagcattc cagtccacaa tatttaccac
    13501 atctttaagt tttaccttaa tcccctttcc aacctcatat tttttatcta taaaacagtc
    13561 cataatctta tggaataaaa atacagttgg tgacattagt atattcatag ttgcactctt
    13621 aaatagagca tttgcaaaag ctattccctc aaatggaagc ataccatcca cttgtgcttg
    13681 acctacacct gatgagtaga ttgacattcc atatgtggta actacaccta ttattcccca
    13741 aactattgct ctataaaata ctcctactgg tattttccaa tccttataca aaattctagc
    13801 accaagtaaa tctcccattg tagctaatac agcaaacttt aaaaaacctc caatatatgg
    13861 atgtgcacct gttgcttcca taaatacttc tctactactt ggtattgcta agacagcaat
    13921 aatacaaata agaactccac tccatataaa atcgcctttt ttcatattta taaatactat
    13981 ttaatatata aatagttctc ctttctatta aagatttatt taatttacaa tctatctaca
    14041 attatttatt aatcacttat tattagatat tcattttcca tatttaaatt tattctatcc
    14101 ctttatctat ttattttctt actactctat ctaccattat ctatatattt attttacgat
    14161 tactctttca ccctaaccct ttaattatct atttttttct gatattctta tctaccatca
    14221 tccatatatt tattttacga ttactctttc accctaaccc tttaattatc tatttttttc
    14281 tgatattctt atctaccatc atccatatat ttattttacg attactcttt caccctaacc
    14341 ctttaattat ctattttttc tgatattctt atctaccatt atctatatat ttattttacg
    14401 attactcttt caccctaacc ctttaactat ctattttttc ttactattct tatccacatc
    14461 atccatatat ttattttaca attactcttt cactctaacc ctttaactat atattttttc
    14521 tgatattctt atctaccatt atctatatat ttattttacg attactcttt caccctaacc
    14581 ctttaactat atattttttt ctggtattct tatctactat tatccgtata tttattttat
    14641 gattactctt ttaacctacc ccctatactt tatagtaata tgtttttaac ctttttcatt
    14701 attcccaata tgctttataa ctacaaatac tctcccccct taaaaataat aataaaatta
    14761 atcaataaat ttataatcat attattactc tatgtattaa gcaattacta tgccaataaa
    14821 ctttgatttt tttaaacaaa tcttaaaaat cctaaaatgc taattttctt taacttttaa
    14881 tgaatctaat agataacatt taattttcgt tatacttttc agaatatttc tattattatt
    14941 tttaagaaaa aaaaataatg ttaacatttc ttaacataca ttaaaaaatg ttaacattat
    15001 aaattgggtt taatactttt actttgactc gatttacttt tattctactt cttactttat
    15061 ttttaactta gactttattt ttttattact tatttgagtc ttatttattt tattctcact
    15121 ccatatgttc aattacattt gtctaaattc tttgatactt ttattacata attttaacaa
    15181 gcttaagtgt tatttgggta gtgcagttga taattattta aataatcatc aactgcatgt
    15241 ttatatcatt acagtagctt aatgtattta atcatactta cttaggattt tgaaatatct
    15301 tagagattat atttaacatt agtcaattgc tgcataattg taagcaattg aaatttgaga
    15361 cccatcttca ttttctatag tgataggctt cataattttt aatccttcag tttttatctt
    15421 ttctaattct tgttccattt tcttaattgt ttcctcaagg tctttttgac ttacatcttt
    15481 gactgcatta gggtctttag catagtcttt taattcttga atatgtttag catactcttc
    15541 ataagtataa tgctcaacac catacttttc caattcagta gggtcagtta ttcctgattt
    15601 tattagagat tcaaatttag gtaaagcaat attagatgaa tcatttccat ttgcaaatac
    15661 tgctccagtt cctcctagta ccattgcaga taccatcatt gcacttataa gggatttttt
    15721 tattttcata accaatcttc ctttcttaga gatttatttg ggtagtgtag tttaaaatga
    15781 ctattgcaat agttatcaac cacattctta gactatcaca gtagtcatgt ttatgtcaag
    15841 cctttttaaa aaacttttca ataaaaaata ctatctcaaa acaaaattaa ttattttgag
    15901 atagtatttt aattctaaat cttttatatt actaagattt atggttactc tatccatgtt
    15961 ctatattgtt ctttattaac tgctaacaaa ttcccttttc ttgatatgac aaagcgatta
    16021 tcattatagc cacttcgctc aattgaaact tctcctgaat cagtataaag tacatcctta
    16081 ctattactag atttatttac atacgtttct tcactattct cagaattagt tactttgctc
    16141 atagatgatt cttcatgctc tctaaattca attacagata cttttgccgt actaaaatga
    16201 gtctctccat cacttttagc tttactaaca agaatatcag attctacttt gggagaatat
    16261 aaatcagttg atttactact tattgaaaat ccaattttat cactttgttc attttttgtt
    16321 aaaccaaaag attcaacctc attaatctct ttattttctg ttgcaaaaac aagacctgtc
    16381 ccacaaacta atacagttga aactaaaaaa gtaacataag ttgatttttt aaatttcata
    16441 atattaacta ttctcttttc tatttttgtc tttccaaatc cactacatat tgtcattgaa
    16501 aatggattat tctcctctgc tagacttatt agtgtggttg cataatcagc cctatattct
    16561 tcaccaagta tactcataac cttttcatca caactaaatt ctatgtcttt atttagtaaa
    16621 aaaaacatca cccagactaa gggattaaac caatggacaa caagagctac aagcataaat
    16681 aactttaaca gattatcaaa gctattaata tgaaccattt catgagttaa aacaaagtct
    16741 aaagtttgat tattagcata attcatttgc tttggcaaaa taattcgtgg ttttaaaata
    16801 ccagatgtaa gtggtgtcct agtaaattca gatatgcaaa caataaatct tctaaaacgg
    16861 ctattcttac acatccattt tttaatgaac tcatgatttt ttatatgaat tgaagtttta
    16921 aaaagattaa ttattttaaa atatctaaag cataaaagta tgctaaacat aatagtaact
    16981 ataaaagaca aaatgcccaa catttccaac atcataatat tttcaatatt agacattatt
    17041 tcaaattcgt cagaattgtt tttacctgta gagtcactag ctttcttaag agtataattt
    17101 tctgttgatg tattacccaa ttccacatca aaagtcgatt caattatact ataaatacta
    17161 aaaccagaat ttaaacttac tggtattaga agtttaaata aaacaatcaa ccacaataat
    17221 acaaatacct ttttaggtaa ttttttaaat aaacatgctc ttataaaaat aactattaaa
    17281 agtaatactc cagaagtaaa actcatttct aacaatttaa tgaacataaa tcttacctca
    17341 ctttaatttt tcaacaattt gtttaagttt tgtaatctca tcacttgaaa gattttcttt
    17401 attaataaaa gctgaaagaa atttttctga agaccctcca aacattttat taattaaagt
    17461 ttctgtttcc tgtttttgta cttcctcttt gcttattaaa gctttacaaa caaaattcgg
    17521 ctcaattctc tctattgcac cttttgacac taattttttt ataacggtat acgtagtgtt
    17581 tctattccat cctgtttctt cttttaaaat ttttactaac tctccagcct tgctttctcc
    17641 tctctcccat agaacttcca ttactttaag ttctgaatca aataatttta tctccatata
    17701 aaatcgctcc tcaaatatag ttatcacaat agtcattact ataataaaaa actatcatag
    17761 tagtctatac tatgtcaatc atttattttt taataataaa aacttactaa atttctgatt
    17821 atcttttcat aagttggtaa atgccgatat atcccgtatt ttagggcttt atcggtattt
    17881 tcttttcgga agataccttg cataagctgg catttaccag catgaaaatg caaccaaaag
    17941 tagtaaatga gtagtaaata tacgttccaa tattaacaac ccagcctttc cagttccgct
    18001 ttagcagatt ggaatgtacc gtgtgcgtaa tagcccagcg tcatggttat gttagcgtgt
    18061 cccatgagat attgcagagt atttgggttc attcccttat ttgccatatt cgtacagtaa
    18121 gtatgtctta atgaatgagg tgtgatgttg ggtaatttat ccttgtgata cttgttatac
    18181 ttcttaatca gccctcgcaa catacctaca tagtttcctg caactttagg caatccctca
    18241 cggttcaaga ataggaaatt gctgtaacca cctacaatca atggttgagc ctttcctctg
    18301 ttcttcaata tcctttggag tgcttgatac gctctttccg tcaatggaag ttctcgtttt
    18361 ccgttttttg tctttggtgt ttcaatatag tagccgattt ctgtatcttt caataactgg
    18421 tggtctatat tgagtattct gttctgcata tcaatctgtg tcgtcagtcc gcaaaattca
    18481 gaaatacgaa gtcccgtttc cagcagaagc acaacctcat cataatactt actgtaaatc
    18541 ttgtccgttt ccataaaggc aagcaggcgt tcttcctgct ctgatgtaag gataactttc
    18601 tgctccgtat catcttccag aacatcactt agcttaaact caaacgggtt ttttctaata
    18661 cagtcgtctt gtatcgccat gtaaaatgac gctttcaagg aacgcttata gttatcaatt
    18721 gttttatagg catatccttt ttcactcatt ctgatagccc attctttagc gtccgactgc
    18781 ttaacagtat caatcgccct catacccaac gggtcatttt tcagagcgtt cataagatac
    18841 tgtcgtcctt tttcagtagc cctcttgatg ttctttcttt ggctgttctt cttgtcaaag
    18901 agctggcaga ctgtcatttt accgccgact gtgtcgatac cgtcattaag gtcttttttt
    18961 atctgtgctt ctttttccct caacgatata tcatcacgct ttccagcagg tgtcttgtct
    19021 gtcggtacaa gtttccaaga atagataaat tgtggctttc cgtatatatc ggtatattta
    19081 taaacgtatc ttccgtcttt tcgctggctc tctccattac gcaaattgcg atttttactg
    19141 tctttccgtt tcacattaga catagtgtaa agctcctttc cgtcatggaa tgagccgtga
    19201 tacgctatac cttattatac catatctaca gctcaatgac attagatttc gtccaatgta
    19261 tctatgattt tttcaaattg ttttcgctta atctgaatac gattaccgtt cacgataacc
    19321 cagccagcgt caagattttc ttcggctaat ttacgtaatt tcttttcgcc aatgcggaag
    19381 tattttgacg cttcctctat tgtcagcgta tatttttccc agataggcac atcagtattg
    19441 ttcatattat gcaccccctt tactgtgtgt cattttttta caagcaggca gacggcttcg
    19501 gaaagctcca ttggtatctc aaccattccc attcctgtgg gcagaaccgc accatgcgga
    19561 cgtatcatta tcctgcaata caggtcgtgg cggaacggct tttccaaagg tcgctctcgg
    19621 atcactgcgt ggatcgctcg cttcctgttg gaaaggtcat ggcgtacagt ttccgtggct
    19681 cgctgtattg caaacgtatc tgtctgcttt attcagtttt caaagagcag taggcttgtt
    19741 gttacgaagc attttatttg ataccgtaat ttgcagaatc cggaaaatta aaatatccca
    19801 tttttagcct tgttataacg gtaataattt tttctattac tgttataaca aggattccag
    19861 gggttcgggg atggaagatc cccgcattat ttctacacaa tttaattctc cgaaaatgcc
    19921 atattataat gtgctggtca acagcttgtc agcctgtgaa acctatcagc ttttgatagg
    19981 ttttcataga ttgacaaatt ttaatactag gacaggaaag agggaaaatg tcaaatcatg
    20041 tccttgatgt gtttatttta tttccacttc aaaatttggt atcatcagca taatcgcttc
    20101 acgaataagt ccctttaatt ccatgtcaat ggcaaagtaa acatttccat aactgtcaaa
    20161 caagggacgc atactggctt ttgaaatata cgggtcataa aactgtaaca gcttttcaat
    20221 agccttttcg ttgctgtcaa cggcagaagc aatcagatga tagggtggac atttctttac
    20281 tgatttttcc atttcctcat tcctccgttt cgctcttttg caaaagttca cgcattttat
    20341 tcaaggcgtt ctgcctgttc ttaaatgcac cagctcttga aatcttttga agttctgcta
    20401 tttccgtatc gctcatatca agaaaataga acatcagaag attattgcgt ttcttttctg
    20461 gaagtctttt caatgccttg catagctcat ctccagatac acgggcttcc atgccatata
    20521 catcaaatac ggttacgtcc atttcatagt catcttgtat agcaaaggaa tttaccttga
    20581 tttctggaag ctcacagaat gatttttcat ggtctgagag aaacttcatg tgcctgtcgt
    20641 aatctttgac ggtcgtgtca ataactcttt ttaccagagt atcaaactgc aagcggattg
    20701 cattttcaaa tgtggtcggc ttcatcatta cacctcctcc ctgtctgctg tgaaagattg
    20761 ttctatccct ctttccacac caacactcga ttgagaggtg tgatttgcta accaattttt
    20821 gaaaaaattt aaaaattttt tttgagcatg aaaaaagccc gcacaaaata acggttctgt
    20881 acgagctgaa aatacggtat gtatggttgt taaggcagtt tgactagata aactttttat
    20941 agattatgtc ttgtgtttcc attatgattt tgctcctttt gtggcagagt ttatactttc
    21001 cctatataac tatcttatca accaaaactg tccaaattct gtctaaatta tcacttttcg
    21061 gcaagaattg aaaaaagcca ttgaaattca acggcttaat attcctgctt attctctttg
    21121 ataagtgtac gatagtattc ctgcctgtcc tctggtatct gggagcaaaa atcctcaatc
    21181 catttgtcat atttctcata gatttcttca tcatcataat attctggata ttctaaatag
    21241 atttctcttt cccgttcctc atgttcttca agttccacag ccaaatccat gtgttgactg
    21301 caaataagct gtgtataggg tatgaagccc agtgcaatag tgatatgcgt gatacgttca
    21361 aaatcttctt tcttcatttc ttctttcccc gtcaatacag ctctgtcata agcagaacag
    21421 atagacagga agattttctg ttcttcttct gatttcttca tagccagttc tccatttcta
    21481 aggtctgaaa ttgtttgttg cgatacataa aaattcttct ggagtaggtg ttttgtttcc
    21541 gaaaagcctg taaaattctg tctctgtgtc tggggctgga ttgtttctgg cttcttcaat
    21601 cgcaatttcc aattcctctc gaatttcgga aacagaaatc cctcttagac gtgctacttg
    21661 ctttagtatt tttttgctgt cccgtttggt atatcttatc atgtattaca ccacctcttt
    21721 tcttgtaaac tatcatacaa cataaacaaa ataaataaaa atataactta tacaatagtt
    21781 acctttatta gtctattata aagcatttct acaaaggtta caatgtataa gaatagtttt
    21841 tatgaaagga gtgtgccagc tatggagctt gattataagg caatcgggaa aagaataaaa
    21901 atcgctcgta tcaagaaaaa tcttacccaa gaagccattg ctgacaaaat cggtatcact
    21961 ccgcaacacg tcagcaatat tgaaactgga aatgcttcgg tcagtctaac aactctagta
    22021 gcaattgcaa acacactaac tgtttctgta aatgatttat tatgcgatac ggtcttgatt
    22081 tcaaaagccg tatttgaaaa ggaagcaaag gaattgtttg aggattgcaa tgagtatgaa
    22141 atccgtgtac tggttgatgt attgaaagca acaaagcagt ctttacggac agttaaatta
    22201 tttgaagata agatagaaga taacagataa aaaccgtacc tgtcagatca cattagtgtg
    22261 aaaacagtta cggtttttct tcactgaatt tgtagcccac accccagaca gtgataatgt
    22321 atatcggatt gtctgggtcg ggttctatct tttttcgtag tttacgaatg aacgagggaa
    22381 gattgctagt atcttttggc atatcttcaa atccccatac attttcgtaa agctgttctt
    22441 ttgtaaacac ctgtccctta tgagaataaa agaagtataa taagtcaaac tcttttgaag
    22501 taagaaatag caattctcca ttcttattta caacgcttct tgtaaaaggg ttaatctgaa
    22561 attcggataa ctcttgagta ataagaatag aagaattaaa agatttagag gttagccatt
    22621 caatagtctg ctgaaaaatt atttcttcat tttccttaaa ttccactatt aaaattttcc
    22681 cataccaacc acctcctcaa ctaacacatg ataaaacata agttttcatc acttactata
    22741 ctcataaggc tcatttgttc actatgtaaa tctcaaattt atctactctt atatctagct
    22801 aatttaaaaa aatggttcaa tatacttttg cacaaaatct atacggtcaa atattgttgg
    22861 cttgaaatat gaagtaattg ctatcactat attatttgac gggtttacat atataacatt
    22921 tccgctatta cctattgctg aatatatatg cttattttca tctacaatcc accataaata
    22981 cccataagac atatttctaa attcttctcc gcatttataa gtagtctttg tcatttgttc
    23041 tatccattct gacgacacaa tttgttttcc gttatggaca cctttatcta aacaaagttg
    23101 tccgattttc gccatatctg tcgcagataa acataaacca tatcctgctg ttcctacccc
    23161 ttttgggtcg caaaaccata tattcttttt cggttctttt gatatagtaa aatgtttatg
    23221 ctcttcggcg gtttccgcta aataatttat atgttcttct actccaatag gttcaaataa
    23281 atacttattt gcaaagtcaa cgacttttat cccactcgtt tttgatataa ttccagttag
    23341 tatatgtatg cctaaagttg agtatttgaa ttgtccagtc aatccttttc ttccacccag
    23401 aaaatctaag gctgaaaccg tccaatcatc acttgaacat attttagtcc acggctcgta
    23461 tttatactta taaggtgcgg tcatagtcaa taaatgcttg attgtaacct gttgaattgt
    23521 tttttcccct cttttagttt tatattcggg gaaaaagtct aatacaggtt ggtctatatt
    23581 ttctataaac cctttatcaa gtgctattcc gacaagtaat gctacaatac ttttagttgc
    23641 agacataaca tggcaggtat ctattttttt ataatcattc cattcatctg aataaacctc
    23701 tttaccgtcc ttatagcatg atatttggca gatattaggc tgtttttctt taataaaatc
    23761 gtgcagttct tttttattca tactatcaat ttcatctccg tttcaagtta tcatttatga
    23821 aatattcatt caaaaaatac aatgttttca tatattttct tatttctttt ttactggata
    23881 ataaatttct gttactaggt catcttcatt tgatgtttgt gttgggtcag taatataaac
    23941 ttcataaaga ggaccattgt tttcataacc gttttgctct gcccattcac actgtttcgt
    24001 ataaatagat ggtaaattag aatatccgcc atatagagtt gtttttaaac atagtcctgg
    24061 acaaaaatct ctagttccag taacaaattg ttctactgga atagcaaatt ctgtatctaa
    24121 tcctaatgga gtaaactcat cactatgaaa aagtaccata ggtggagcat tgacagttaa
    24181 tttattatat tggattttcc tcaatattga atgaaagcaa caagcatatt gttcttccat
    24241 ttcaacctta caaaccattt tacgaataga aactaaatac ataactggta cttcaacaag
    24301 ctgtacatca atcttattca aataagacat gattgatttt ccttgtttca aaactgatat
    24361 atcttcattt aaccgttcta taatttggga atatatctgt atttgttttt caaactctat
    24421 ctttttctta tatagctcta tggataattt ttcattcagt atttcttctg atgtgataat
    24481 tgctttaatt tcttccaaag aaaatctgta ctgcttcaga cggttaataa acaacatagt
    24541 ttctaattgc tcaatggaat aatatctata actgttctct tgattgattt tgctgggttt
    24601 tataagccca atttcatcat agtaacgaag tgtctttgta gaaactttac atatctttga
    24661 aaattcaccg atagatagca taaatcttct cctttcaact tacttccaca cttatgttac
    24721 accttgccgt aaggggaaag tcaatgtggt aattttatat cattattctt tacccacacc
    24781 taaagtaaca tgataagaaa ttgatttcca actttttgct gtaaaaaaac cgtacttgtc
    24841 agatcacacg agtgtgaaaa cagttacggt ttttcttcgc taaatttata gcccacaccc
    24901 cagacagtga taatgtatat cggattgtct gggtcgggtt ctatcttttt tcgcaattta
    24961 cgaatgaacg agggaagatt gctagtatct tttggcatat cttcaaatcc ccaaacgttc
    25021 tcgtaaagct gttctttggt aaatacctgt cctttatgta aataaaggaa gtataataag
    25081 tcaaattctt ttgaagtcaa ggaaacagca ataccatttt tgtctataac atttctctta
    25141 aataagctga attgcagttc ctttgattta ttttctgaaa tggtagaagt agataactgc
    25201 tcagcaatcc attcaatagc ctgctgaact attttttctt cattgtctga aatttcaatt
    25261 agtataattt tcccatgcta atcgctcctt gtaaaataat gtgaaattta aattttaatg
    25321 aaacactctt aatatatatt gcaaatttaa cgtattttaa cctccttagg tataacaatc
    25381 ataagaatga tatatgcaat aatgattttt ggaaaaacaa aaaccgttac agtaaatagt
    25441 aatcttacta ttttagcgtc tacattaaaa cactttgcta aaccaccaca aaccccaaaa
    25501 aagattttcc cttttcttat tctatatagt ttattcatta gcctttttcc tttgtctatg
    25561 gtttaaattt tttaataaga tagctgagat acaaccgcat aagatcaata ttcccaaact
    25621 acaaatataa ttctgaatag ttgtttcgta atacccagaa ataccaaata ttgctgtgat
    25681 aggataaaaa gttctcgcag tatttgacat actcataaat agtccggcaa atgaatagat
    25741 ttctgctaaa agaagagcta accagtaact tttctttaga tatggcacga ttgcaataat
    25801 aggggaaacg gcaaaaaata caccaatacc ttctaataaa tacattttta aaaattctac
    25861 aaataccacc atagataaat cagaaaaatg taaaataact tctgtcataa atgtaattac
    25921 aaaaagtaac aaatacacca gcatactaaa taccaatgca acaatcattt ttgcagttgt
    25981 caattttgtt tcatttactg gaattagacg tagagatttt aatgtattgt cttgttcctc
    26041 acgacaaatc atataacttc ccaaaagtgc aataacggct ggaagaacaa acatagtcgc
    26101 ccaaacttgt gtaattgtca tataccaacc gactgtgctt gtagttaatt gattgtcaga
    26161 caccgttgtt acaccgctaa caaaaacaat tacagcaatt aaaattgtcg caaagattgt
    26221 aaacataata atatttgaac ggcggagttt ttgaaattcc gcccataaaa gtattcgcat
    26281 ttttccacct tactttctct tgcttaattt tattttagca agtaaaactg aagcaattcc
    26341 ccatacgcaa atactcaaaa gtgctaatga tatactattc tgtggaagtg ccaatcctaa
    26401 aattgcttca tttcttgcaa caatgacact aatacaagaa agcgggtgca gacaagaatt
    26461 tatcggtgtg attaagaacc cagcaaacac ataaagcaat gtaatgcaaa tagggaatat
    26521 atatcctttt tgcgaagcag agattgctaa aataggaagt atagcaaatg ctgttaaaat
    26581 ggcaatttcc aaacatcttt caaataataa tagaatgcta ctgaaatcaa atgctacata
    26641 gtttggaata acactaaaca atacagaagc aaatgctgta ataagcataa aacaaacgga
    26701 ataaatcaat acaacgaaaa atttactgaa aaaatatccc attttactta ctggcacaat
    26761 ccaaagttgt tttagcatat catacctatt ttcatcatgc ataagcattg tgccaaataa
    26821 gcctaatata aatggcagta taatgaataa tgtatatcca aatgcagacc acttataaaa
    26881 tagtctaggg tcaatattgg tctgccccaa ataaccaaaa tataaaaatg caaagaatgg
    26941 catgacaaaa gcagatagta acatcaacca aataagtttt ttacgacgca acttgcaaaa
    27001 ttcaagttta actagcttaa gcaataccct caccccctgt aatctttttg aaataatctt
    27061 ctaaggtatc attacaaagt ccagaactga taactgttat atcttccata acaagagctt
    27121 tattaacttc tcccatattc aatgtcgtgt cataaatttg cactgtctgt tcatcttgaa
    27181 ctgaataatc tttcacatga aattgacgct ctaatattag tattgttttt ggaatgtccg
    27241 aaacttgcaa cgaaatatat ttcctatttc gcttttctaa ctcactcata ctgctttctt
    27301 ctaaaagaac acctttatca ataattccta tgtcatctgc aagcactgct atttcagaaa
    27361 gaatatgaga agaaattaaa attgtttttc cgtgttctac actgagcttc tttatgaaat
    27421 ctcgtacttc tgcaatccca atagggtcaa gcccatttgt tggctcgtcc aaaattaaca
    27481 attcagggtc atgtaatatc gcatttgcaa tacctaaacg ttgtttcata ccaagagagt
    27541 attttgcaaa tgtctttttg tctttatagg gcagacctac aacttctaat gcgtgtttaa
    27601 cagcattagg cgaagctgtt cctcgtaact tagcgaaaat ttctaaattt tctgttccag
    27661 tcaaattggg ataaaacccc ggcgtttcta taattgcacc aattctggaa tatatccttt
    27721 tctcatttcc gtctatattt tttccaaaaa cttctacatt tccagaagta gttgttgtca
    27781 atcctaatat cattttcatt atggttgttt tcccagctcc gttacgtccc agcaagccat
    27841 aaatcctacc tttctttaca ttaagattaa ccttatttac tacggtttgt tttccgtact
    27901 ttttggttaa ttgctttgtc tttatcacag tatcactcat acaaatttcc tcactttcta
    27961 tttactgttc tatctaagtg gcaatttatt agaattacca cttacaccta aattatagta
    28021 tatgaacctt gccaaaacct tgccgtaacc ttgtttttat cttgctttca caaaaagaaa
    28081 gctctgcatt tttacagagc ttttggaaat gataggataa attctgttcc atgatttaga
    28141 atactatttg cagtaattgt ccctttatgt gcgttgacaa gttcctttgt aattgccaat
    28201 cctagaccat ttcctttggt agagcgagaa ttatcgcatt gatacaacct ttcaaatata
    28261 tgtggcaatt tatcagaagc aattcctttt ccattatctg ttataataac tttaattcgt
    28321 tgctgatctt tcagaactcg caacatcatt ttgtttccct cactatgaac aattatattt
    28381 tgtaaaagat tgttgataat acgagtgtat gcgtttatat caattcgcac ttggtattct
    28441 tcttctggta tctcaacctc atattcaaaa tgactatttt ccaatattaa aatccaatca
    28501 gtcataatat tacgagtcaa ttcgttcaaa tccaaatttt caaaatgaaa aatctgctcc
    28561 ccagagtcta attttaccca ttcaaataag tgttctacaa aatgtttaag atgttgtgcc
    28621 ttttcagacg caacatgaat atattcgtct ttttctttgc cgattacaat ttgactttca
    28681 accgcttcta aataaccgac taaagaagca agtggagttt tcacatcatg tgaaaggctt
    28741 gtcattagtc gtttgtacgc ttgttccgat tgcttttgat gaatgagctg tgattgatta
    28801 ttgattgcta tttgatttat atcataacaa atctgtttag ttatatcgtt ttcttgtact
    28861 agaatacgtc tgttaaggtt accagatttt atgtcaacaa gcacttcttt gataaaagaa
    28921 atttgctcgt taatatgata aagtttataa acaagacaag cgattattac aagtgctaaa
    28981 acaaaggcaa taagcagata agtattcata attaagcctc cttgttaaaa cggtagccaa
    29041 cccctcgtat cgtcaaaata taaaaggggt gttctgggtc tgcttcaatc tttttcctta
    29101 atttgctgat gaaagacatt atattgttat catcaaaagc atagtcgtct ttccatactt
    29161 gtgtataaat ctgctttttt gtaaatattc gccccttgtt agaagcaaga aatgtaagta
    29221 aatcaaattc cttacttgta agttcaacag aaatattatt aactaaaaca gtacggttca
    29281 ctttatcaat tttcatgcct tttaaagtta tacaatcttc ttctgcaaat gtaggattta
    29341 aagtcgtgta acgtcggata agtgaattga cacgagccat aagttcctta atactaaaag
    29401 gctttgtcaa ataatcgtct gctcctagac gcagtccaaa aaccttatcg tcctcaccac
    29461 tttttgctgt gagcattaga acgggagtat tacttgtttc cctaattttt tctaaaactt
    29521 gaaacccgtc tatatcaggc atcattacat ctaaaataac aagagaaaat aaatttttat
    29581 tttcatctat caaacgtaaa ccctctgtac caccatttgc caccaaagct gacatttctt
    29641 cttgctctat acattttttc attaacgtac ataattcttt atcatcgtct ataatcaaaa
    29701 cacttcccat gttataactc ctctctgcat agattttatc atagcttaat tttcgttaaa
    29761 gtttttcaac attaaacttt tgtctttttt gtaattttgt aaatatattc ttctggcgta
    29821 gggcgtttat ttccaaaata ctttctgaaa tttgcctgca cctctgggtc tgtgctgttc
    29881 atagcttcat caatcgtcgc ttcaatatcc gcacgaacat cagccaatga tacaccgcca
    29941 gcttttgcac cagctttcaa tgcctttttt atatctcttt tgttcagtcc tatcatattc
    30001 atacctcaat tctacttttt aaagctaata agttttccat tgtattttac aatgatgaat
    30061 atgatataaa atactcctac aactatcaaa gtaagcccca tgtaatagcc taattcagaa
    30121 tgagagatta aattgataat cggcataaca agaataccaa tactaaccag taaagagcct
    30181 attcctattt tttttgtaaa caccttttta tctttagggt caagttgtgt ataatgataa
    30241 ccatgtaaca aagtaatatt ctcttttatt cctatttgat aagatgtaaa aagtaaaaac
    30301 aatcctataa ctatttcaac aatcaattcc tctaattcca taatcgttct ctcctttaat
    30361 caaaataaaa taattcttca aattttttat ctaaagctat acaaagcact aatgccaatt
    30421 ttgctgttgg attaaattgt ccagtttcaa tagaacttat tgtattccga gaaaccccga
    30481 ctaattcagc taattctgtt tgtgataatt ttttttcttt tcgtacagtt tttaatctgt
    30541 ttcgcaaaat taaatgttcg tccattctat aaacctacca ttaaaaattt ataaaagtaa
    30601 taccccataa atagaagaaa gaataaagca ataacaaatt tggatttatt ttttgaaata
    30661 tgtgcttcgt aacccatgaa gccaaatgcc acagacccaa taattgtcat tatgtcatag
    30721 taaggttctt tacaaactgc tttaatgaaa agaattaaaa tgcagataaa taaaacaaac
    30781 attaagccaa aagatttccc ttgaagttta atatgttttt cgtactcatc aagatataga
    30841 ttatcttttt tgttttgtga tagtattttt tctttatcca ttatataacg ctcctttcaa
    30901 aaatgccaag tacacttgtt atttatatta tagcattcag tcattacaag tcaatatttt
    30961 tgcacagttt tcttgttatt ttgtaaaaac gtgcaaatct atacagaaat gcacgctttc
    31021 aatgtttatc ctacaatctt ccagttgtta tccttatgta gcacaagctc atactgcgat
    31081 acttgcgtcg cttttgtctg attatcaagg aattttaccg caactttcac ttttacattg
    31141 tccccgtcct ttgtaaagat aggatttacc agttcagaat aaaggtagtc cctgccgata
    31201 ggttcaagta cattaccaga tacatagtag gcaagctctt tttccgtcgc tgtcgggtac
    31261 agcttaaaga atgtttccag aaaagcagta gcgtcattga cggtatcagc gtctacgctt
    31321 gcgtctgctt ctgctgtctt tggctcatag tctgattttt cgattgctgg tgcaagggta
    31381 gggttctgaa cgattaccat atccccgtca gcgtccacat ggacttttac agtataggtt
    31441 gccttgacat tgcttatctg ttctccctct tttatctgct gatctacttc gtaggtagca
    31501 gaaaaagtgt ccgttcccga ctgttcgata ctccatacaa tcacatctgt aactgtggag
    31561 ctggtcggta tatccgttct gattgtatca acattcaagt cttgcaattc ctttgtcaga
    31621 taaccgctga ttgcctgcgt ccttacttca atcgcttctt tgctgttatt ccatgtgtaa
    31681 taggacttcg caaagttttt cacgaaattt tctatcccgt tggtgtcctg caagcgaagt
    31741 tcaatgattt ctttttcatg ggtcgtgtgc tggtcgatag cggtaaaatt cttatacacc
    31801 ccaaagctca cgcttgcgat aagcaccacc cacaacgcaa tcacggtttt cttatgtgtg
    31861 cctaccttga cagtacgcac ctttttttct ttgatatttt cagtcgattt cttattcttc
    31921 ttaaacatat ttttcaagtc ctttctatta ttttactcgt cctgcaccga ttaagtgctg
    31981 ttgccagtaa ctactactta ggtctgcata tcctatcggg tcgcctgcgt ggtacatctg
    32041 tgtcggcgaa acaagaatac cgacgtgtgt tacatacgaa ccagcattat aggtagaatg
    32101 gaaaaacacc aagtccccag cctttgcctg cgagagtgga agatgttggg ttgcgtcata
    32161 ctgtgcctgt gccgttctcg gtaaggagat accagctttt ccataacacc attgcgtcag
    32221 ccccgaacag tcaaaggacg tgttgggatt gctaccgcca tacacatatt tccagccttg
    32281 atattttaac gcttcattca tgaccttttg tgccagttct cccgatacct gcggaactgt
    32341 taaatactga ttgactaatt ccacataaaa ttgattgcca tactgataac gccagccccc
    32401 gtttttcgca acagctatcg ggttggtgta ggttactttc tttccacccg acttctcacg
    32461 ggcgaagctc tctgcaagat tgtaggtatg tttctttcct tttcctgcca catattccac
    32521 atagccaccg ccatagttat aggactgtat cgctacattc aaatcgtcga tatcttgatt
    32581 tttgcaggaa gaaagcaggg acgcaaaata cttgcacccc tgcttgattg agctttccgt
    32641 atctaaagag ttgggcggta aaccaagact ttccgaactc tgcataacat cttctgccgt
    32701 accgccactt tctacttgaa tgatagcaag tagcacattg acatactcgg agataccgta
    32761 ttctctggcg tatttttcta ccataggctg atgtttcaag acttctgcgg ataggttcat
    32821 acccgtaatg ccagaagaaa aattgctgtt ctcgtcgtcg ctgtccgcac taatcaagac
    32881 accaaagaaa agcaccagag aaaagaggat aggaaacaga ctgccaatga gagcgatatg
    32941 tttcagtttc attttttctt ctgtcctttc ttcgtgacaa ggttacgggt tttctctgtt
    33001 gtctgctggt tcttctggac gctctgcgtc tgctgaacct ttgtcctgcg gtctttggtg
    33061 tcattctggc gtgtttcctg cgataccact ttttctacat tctgcctatg tactggctgt
    33121 gctggttggg tcgctttctt atcagaagca cccgaagata acggacgctc tttcacaaca
    33181 tttgtcttga ctggctcact tgcttttgaa gctggagctg tggttggtcg ctcatgggga
    33241 cgggtagctc ctgttgtcgc tgatccgtca gcctttcgct gtgcctgcct tgcttcttgt
    33301 gctttttgaa gctccatacg cttgtcagcg atatttttct tacgctgttc ctgcttttcc
    33361 atacgtcccg tttgtctgga ctgctgttcc tgcaccatgc cacgcttgaa gtcggacacg
    33421 ctggactttg ccttttcctt tgccgaatac accgcataag cggtctgtgt cggcatatcc
    33481 ttgatatttt ctttgacagc gtttgctttg tctttcactt tatttttcgt atctaagaca
    33541 gcacccacct ttgagcctgc acgctgtccc atgctggaag tggtattgcc ccgattttct
    33601 ttagaagcca tattttttct ttcagccctc ttgccagcaa cggcacttcc agctaccgca
    33661 ccagcgacac cgcccgaaat accgccagca cttaccgccc ttgcaatacg gtgttccata
    33721 cgcctagccc gatgtcgcat aaacagatac ggtctgcgga atatgcgtcg tcccatgctt
    33781 tgactgtcgc cagcattaag tgagaacata ctcattaagt cgcccagttt catatagata
    33841 ccagcaaaac acactatctg caagaacgcc accatgaaaa acgggtagtc cgtggagatg
    33901 ttataaaaca tactggaaat gctgaacgcc accgttacaa tgagcgttat tcctgcccgt
    33961 gtcattatgg tattaaacac ccttacgatt gcctgctttg ccatgctttc atagctcggt
    34021 atcatagaga gcagaaaact aataggcaag aacatagcga aaatgataaa aagtatctgg
    34081 ctgaacaaca tcatgcccgt aagcaaaaat acaaatattg ttatccctag attgaagaac
    34141 aagaggaaaa acaccatacc taaacggttt accacctgcg gtattgtcag attgttgttg
    34201 tcgttatcct cgatttctgt tttcacgact tcctctctgg tctttccgtc ctcgtcctct
    34261 gggcttgccg atacgagagc ttccacacgg tctgtcccga tttcctctac gttgctgttc
    34321 ccgaattgta gaagtagcca cggctgttgt acttgaatag aaaataagct gtcccgtatc
    34381 aagtccacgc tgtccttgcc ctcgctgtca gagtttggga gcatgatttt tgttcccaag
    34441 tccaaagaag cggtgctgat gtctgatgaa aactcattta tcttcttgat gtagtcggga
    34501 gcataagcaa taaatgaagc ggacagcacg aacaccacaa caaagttgat aacagcgtga
    34561 agtgccttgc tggtttccct ttttatcagt cccgtatagg ctacataaag tcccaccact
    34621 aagataatga gaagcaggaa accgacataa aagccatagc tggaaaaacc gttctgtgta
    34681 acacctgcaa gggtctgtat gctttttccg atactgtctg ccatatcgtt aataaaatca
    34741 agtttatagg cttcctgcac cacatagccc gttgcattgc ttaaataaag gcttatcgtc
    34801 cagacaaagt tggtgataca gtaaagtccg tactgtactg attttccgat accgtccagc
    34861 cagttccacg gaagccacga ccagctatta tccacataaa aatcaagctg gtagttggaa
    34921 aggggatatt ttgagtaaag attttctgcg tttatggtat cgtccacaag ccccgtcgca
    34981 tgagccaccg tccccagaag tgaaagcagg ataagggaaa gtgccacgac gaacagagcc
    35041 attttgagaa agtggaaaat cttctttttt gtgaacgcac cttttatcct ttctttcatc
    35101 actccacctc atttctctgt acgggcggtc tggtatcaaa ggcgtgtagc agttcttcaa
    35161 agactgggtg tatctgcacc acaccgacac gcccgtataa gtcctgcaat aagcattgtc
    35221 cgttctccaa atcacgaagc cgtttctggt tgttctcgtc gtctttgtca ataccgaaaa
    35281 attctaaggt ctgctttatc tcgttaatat cagtagaacg gaaagcgaat tttaagccga
    35341 tattgttttt cagactttcc tttgatacgt ccccagagga ttgggtaacg aaataaacgc
    35401 ctgcctgcat agcacgtcca gcacgaacta acttgttaga aagtgtttcg ccttgtgcca
    35461 catttaagaa cgcccacgct tcgtcaaggt ctacaatttt gaagatagaa cggtcgctgt
    35521 gaataaaatc taaagcaaag gtactaatca caatcagcat agacaccgac aataattcaa
    35581 tggtcgtgta ttcctcaaag gtcgtgtcct tatctggcaa tacaaggtcg gctacttgaa
    35641 tgatgttaag ctggttatcc agactgattg cattttctac cgttccgtct gaaaacagca
    35701 ggtgtgcaaa atcatagtcc gtgaagctgt cgatatggtc tgcgatattt cttgatactg
    35761 gcgtatcttc acggcgtagc tcgtctatca catgaagcaa gccccgactg tcactctggg
    35821 taacagaacg caccgcctta cgaagtacgg ggaatttttc tccgtccctt gaagaaatac
    35881 ccgtaaggaa tgttaagatg tcgattgcca gactttcagc gtcttttaca ttcttcataa
    35941 tcacaaacgg gtcaagaagt cctgcattgt ccttgtcgct ggtaagattt acgatattga
    36001 tttcatgggc gatttctggg agcgtttctt tccagttgcc acgctcggat tttgggtcta
    36061 agataaccgc ttgtccccca aagagaaccg aataatacac cagaagattg ttacagaacg
    36121 atttcccacc gccaagcgaa ccgacaaaag cagacgctaa agcgttggta actgtacctt
    36181 ttacaccttg actggcaagg gacggttgca ggtacacatt tcttcccgta tcaacggaat
    36241 agcccatata gatacccgta gtttccccta actgctgggt agcaccgaag cccagcccag
    36301 ctaaaaaatc tgattttaca tactgcacat agtcattgat atatcgcttg ctggcaggta
    36361 aaaattcaga atgaagcccc agcatatccc cagcaggacg caccagcttt acattaaggt
    36421 cgtcgtaaaa gtccttgact tcatcacaac ggcgtttcag ctcgtcaaga tcgggtgcag
    36481 acacccgtat cacgtagctt aacttataca tactttcttt gctctggtct aagtctgttt
    36541 ccagctcgtc cacgctgtct aatgcgtcca ccacatttga gctggtttca cttcctgctt
    36601 gataagcgtg attgtcaagg tctttcaatt cctttttctt gttgcggact gtcgttaatg
    36661 ctttccgatt ttccacgatt tctacattca tagaagtatc aacggggaat gtgaattgct
    36721 gttgctggaa atagaagatt tcacttgacg gaaaatcaag ctccccgaca atcgcattga
    36781 cagtaaaata ggacacataa ctttccttgt cctcatgttc caatcttaaa taccgctggc
    36841 tttcctcaat catacacctt gtcggacgga taaggtcgta gtattttatc agcgtttctt
    36901 tctgtaattt cttcttcggt agctggtatt cataatcttc ataggcgata ccgtccctgc
    36961 cgtaaagatg ttccatgaga tacccaaaat cgttgatttc caagcgacgc accttaaaac
    37021 gacgggagat tttattttcc agtaactttt ccattttcat gtaacggttg atttcatcat
    37081 tcggcatgga aacaaagtca ttcatcagcg tgtggttcac ttcatggaga aattccgtga
    37141 acgtcagcca cgccgatttt ttgatgttct tcagattgag ctgttcttcc gtaaccatga
    37201 gcttaaagcc aagaaaaaag cggtagtcca cttgattgtc cccaatcata gatactaacg
    37261 cttcggtctg ttcgtctatc ttctgatagg caacttcctt taattttcca gttaccagct
    37321 tctttgactg ctcctgctga cttcggattg agctttctgt ggcaatctgc aacgcatgaa
    37381 ttttaccctc acgggactgt gcgattagct gtcgaaagct gtcatgcacg ataaatttct
    37441 gctctgcgga taagaaagaa tagttatacg gtatcagctc atagtaggcg aatacctcat
    37501 tgtccttgtt ccagacaagg ttattgtcaa tatattttat cgggaacata gttcacactc
    37561 cttactgctg tgattgtttc attcaaggtc tgcttatgca gttttacggc ttttcctgca
    37621 taagtgattt taggtcgcag ggcataggtt atctgtgatt tcaaaaagct gtacggcttc
    37681 tttccgtcaa aggttttctg cgacataaac caagtgagag caacaggaat accgaagtat
    37741 ttgagaaatg ctccctcaat catggaaagt ggaggaatat ccccaaacag aatgatgata
    37801 aattccgtaa tcacaaacca tgtaatctgt gtaaaagtaa cgggaaaggg taagttaaag
    37861 tcattgattg catacaagac tttttccacg ttccagatac ctgtataaga tttaatcttt
    37921 ttcaagttct ttcagctcct ttcaaaatag aaaaggacag ccattttcag actgtccctt
    37981 aaaagagaaa tacgctgtca gtagcaacaa tgcttgttgc tgatcttcca gcgttagtat
    38041 ggtatctcgc acatacctcg gcttgtttca ataaagcaac cgcccatatc caaatctcgc
    38101 ccgtaggctt catagtcaat gtagttctgt aagctgggtg gaatgtcgcc aagtgcctgc
    38161 aattcgtcta tgtagtagta ggcaacgtct gtcatggttt cacagtcggg ataataataa
    38221 atatcgtcct tgtgttccac gacttcttcc agcgtcccgt agtggctgat aaattcgtcc
    38281 agacactcta cgatatagtc gggaagttcc tctatcattt catacatctc attgagttct
    38341 tcaatggaaa catactcgcc aatcgcaatc gggaagttgt cggtatcatg gattgcgtat
    38401 tcctcatact gttcattcaa gccgattttt tctttcacat cttcttcgtc aatggggaac
    38461 gtgaaccaag caccgactaa ataaccctca ttgtatttac caagattagc aatataaacc
    38521 gccatatcat caatcataag caccacctca tttcctactc tggcagttca aagataccgt
    38581 gttctgttac tgaaaatttc ccgtgttcct gcaagtgaga agcgtaggct tcaaaatcaa
    38641 agtaatattc ttgacagtcc tcggacagat gtttgaacgt cgggtcgttc atcagctttt
    38701 gccttgcaac atctatcatg cttttgcagt ctggataaac ggtaatcacg ttcctgcaaa
    38761 tataaagtgc ttctaaattc tcaaacaccg taagtagtga tacatattct tcctgcatat
    38821 cacttgaaag ctggcggtac ataaaatcta attcgttaag ctgatacacg cttgtatgtt
    38881 cgtgaacttc atcagcataa ggcaacacct tttcgataat gcggtaatcc ccactttctg
    38941 caccgatacc taacttttct tcaaactctg caacatctat cggcaggtcg aaccagtatg
    39001 atgttgtttc ttcgccagtt gttcctttcg tttccaccag cacccttgtt tcctgcattg
    39061 ttcctcacgt cctttctgtt gtttcatcac atcttttatg gtctggtacg acataccaaa
    39121 cacatacttt gaaaaatctt ctaactgtct ttcttcatag tcagcaaggt tcagccgttt
    39181 catttcatgc cacaccttac gcttgtaaga gggcaggtcg gaaagataat cacagccgac
    39241 ttttgcctgc atatccttaa aaatatcgct catttcatca ctccaatctg aaattttggt
    39301 aagaaaaaag cagtcaatcc taagactaac tgctttttaa aacatataag aacttatatt
    39361 ttattttgag aaaaacacac cataaagaac gataagtgaa aaaaatatta gcactccagt
    39421 tccaattata taacatagtc taataaattt ttttccctca tttatatcat cgtatatgga
    39481 agataatttt tcattattta aatttttttc tcttacttct tgcacgtcaa tatctcttac
    39541 cagttcatct aatgtcaatc caaagaaatc actcaataat actaatcgtt gaaaatctgg
    39601 gtatgattga caagactccc attttgaaat tgtttgtctg gaaaccttta gttcatttcc
    39661 caattcttct tgtgaaagac ctttggactt tcttaaattt atcagttttt cattaaagtt
    39721 cataagtagc ctcctaaaat aaattatatt tgcgaccgca aaatgagtat tgcaaatatg
    39781 atgatttctt tcaaccaacc tttgttggca atttgtcaac cattcattac atcagtataa
    39841 ttcactaata tttgtcataa agtaaagaaa tattaaccac atataagttc atttttcatg
    39901 ctccaattat agcattctca tatcagcacc acaataaaat tttacgtacc gataatccta
    39961 ttgaacagct ccaacagaca ctatttcatt actccaatct gaaattttgg taagaaaaaa
    40021 gcagtcaatc ctaagactaa ctgctttgtg tgtatggata tgaaattgtt tatcagacat
    40081 attgtttgaa aaatacattt aacttttgcc ctagtagtgt tgggttatct gtatttactt
    40141 catgtcctgc attttcaata atagctattt ctgcgttttc tatctgttgt tgcaattcta
    40201 aagaagcttt tttatttatc ttatccttat cgccacagac aacaagtacc gaacatgata
    40261 tttttttcag ttcatgttta aaattaagtt ctgtcataga tttgcataga ttgataaaat
    40321 ctgttttctg aaagcccatt ttttcaaatg ctgaacaagg cataatacgg aatatcagat
    40381 tttgcatttt taacagatac tttggcattt catattgtgt gcctattaac actaatgagt
    40441 tcattttttc tgggtgttct atcgcatact gcatagcaag aataccacct aaagaaagtc
    40501 cacaaatatt tagcggttct tcaaactgtt cacagtatgt ttccaatgtc cgatacaatg
    40561 tatcataaca tggtgttttc ccagaaagcc aatctgataa attagggcat aaaacttcaa
    40621 aatcatcatt gaatactttt acggtatcac tccaactttc agaagattgt cccaaaccat
    40681 gaagcattat atatttcatt tcatcactcc tagatactgt gtttcatatc cacattataa
    40741 cattctcata tcagcaccac aataaaattc tatgcaccga taatcttatt gaacagctct
    40801 aacagacacc atttcatcac tccaatctga aattttgata agaaaaaagc agtcaatcct
    40861 aaaactaact gctttgtgtg tggggatata aaattgtttt taatcttgaa taggtatcca
    40921 aatttcaata actgaattat ctctattcca atggaaacgg taatcatatt tttcaaagtg
    40981 caggataata ttttttatag gggtatatgg tgtattagga ataatttcct gataaatttt
    41041 tccataagtt tcatatattt tttccatagc accgatatgc tccatgacca aatacttata
    41101 agacggtatt tctttataca gaaagttgtc tggaataata gttcgctttg gtatagaaca
    41161 gaaataataa agttttccat ttctccgttc cataaaagca tattttaccc aatttcccga
    41221 ttgtgaaaga tatgatttct tcaaattact gttaaacttt ctccaaaact gcgtactaat
    41281 ctgaatattc cttttttggt aattcgtaag ctctacttcc tgcccaataa ctgaaaatgc
    41341 gtctaattct cttatagaat aattcatcat tttactttgt gagcagtgat aatggtataa
    41401 ctgtacgcat ttattgtaat gatacaatta tcaatcgtta tgtaccagtt tttccctttt
    41461 cttataattt ctgcattaga aagactgatt ttttctttgc accattcaat cacgttttct
    41521 acattcaaag ataagttctt tttaattcta ataacgccaa gttcagttgt atgaagtcta
    41581 tccaagtttt ccagtaattc atttgccata ttttgacacc tcatttttca acagatttat
    41641 ttgtatgttt tatatcccaa ttataacatt ctcatatcag caccacaata aaattttatg
    41701 caccaataat cttattgaac agctctaaca gtacgtcttt cacaccgcca gcattgaata
    41761 ccaagccgac agcaattaag gcaactacca aaaagccgat aagtttggaa aactcacgct
    41821 tgaaacctaa gtagataccg ataaccacaa tcgccataag cactaagctc tgtgcgtttg
    41881 ataaaaacca ttgatacaaa ttttgcccga aattcattta attgtcctca ctttctctta
    41941 attgtttcat tttatagtcg gcttctttgc cgacgcttaa catacacatc aagaccacgc
    42001 cgatacccat tcccatagat accaacagga agtcttttac taattcccac atttttcatc
    42061 actcctaact ttctactaaa tcttttgcag gggtcgtctg ctgtttgatt atcatttcat
    42121 gcttttctgt gagttttgcc tgctgttcga ttgtttccat gtagtctgta ccgtttccct
    42181 tatcaatctt tttcagcatt ttcaaggttg gtgctacctg cctttgtacc caacgcaatg
    42241 tacggtctaa ggtataaggc tctggctttg tcgtcagctt caaactctgt ctgttatcgc
    42301 cgataaacca agcccagcgg tcattgagtt tccagtcatt ttttcgcttg tctggttctt
    42361 catcaacaaa ccgcacatac tggttgatga tagaaaaggc ggtctgctct gcgtcataat
    42421 aagtcagcaa atctcgtact gcataataag cacgttcatt gcgaagccgt atctcaaaac
    42481 ggttgataat gtcggcttct tcaagcggtg tccctaactt gacgtactgc tcatagtcct
    42541 tttcatagat acaaaaatac acatctgatt tcagcgaacc aagataaaga gtacgtccca
    42601 tatattctct gtcgtcctct ctgtgcttga taagctcgcc cgactgataa aacttatagc
    42661 ttctggactt tccgatatat tcccgtttcc tgcatttttc cgcaagctct ggaatatcta
    42721 aaatgcccgt atggtcgttg atagcaaggt cgatacgttt catcacgccc ccgtctatga
    42781 gtgcgtccat gaggaagtca taccagcttc tttgctgtgc cagcaggtaa ctttcaaact
    42841 gcctgcaacc tcgccccttt aactctaaaa ggacaccttt ttcttcgtca gccgacgtat
    42901 agataaagat gtcccctaaa gaataatgct ccgtataact gtaatgtccg taatcttcat
    42961 ggagcatata attgatattc agttttaata tatctttgat gatatgctgt atatccagcg
    43021 tgggaaaacg aattttcaca taatcaaaca gcatggtaag cggtgcttct ggattgaacc
    43081 ttgccagttc cttatgcaga gtttccagaa gttcctttga cggcttcatt tttccgcttt
    43141 ctatcttgtt gagatattct cttgtgatac cagaagccac cgccagtctg ccttgcgaga
    43201 taccgtaagc aacccgtttc tcccgtaatt cttttatcca ttgttcttca ttcagaacca
    43261 tacccccaat ctgtaaagtg tgaagttttt ttaagcgact tcacaactga attttgctag
    43321 gaaatcatac cagaagcctt atgtttccta tgttttttgt ctattgtcta tttgcaaacg
    43381 tacccctctg ttagataccg aggggttcat cagcagagca gggacaagcc ctgctctgca
    43441 aaacggcttc cacggtcgta tttgctccgc cgaccgttcc tgccgttgtg caggcagaac
    43501 ttatcctacc tgttcatacc aatgtaaacc atgatgaatg aaacatcagc ggataaacat
    43561 aaatttaatg tttcttctga tatttcactt ttacggatag ggtctgtttc cagaaaagac
    43621 ttatcaagca attcaaaaac aaaatcaatc tggctgttgc aagccgactg ttttctattt
    43681 aagcgtatca agaacaaatc gggcatgaag tttttttcgt gaattgcatt ttcaagaata
    43741 gtatcagctt ttttctcaaa ctgatgtttt ggattacatt taaaatcgtg ataacgtttc
    43801 tgcacaaatc cctcgcaatc ggctagaaaa tctccataat tgatacagat acattttacg
    43861 ctcttagatt tctcgtcttt atcttctgat tgtattattt tattgatttc ttttatttcc
    43921 atgagttagt tctcctttcc ttgttgttct ggcataactt gtctttcttg tgcaagcctg
    43981 ccgatagtct gcaaaaaatc atgtcctttc gggactaaag gcgtgtaaaa ttcacttatc
    44041 acgcttgttc ccacatcaca ataaccacgc cccttgatac gcttctggaa aaactgcttt
    44101 ttcacgtctg aaccaaacag cataccgtaa cctaattcac tgatacgccc aagtcccaca
    44161 cggaaattga agttatctct gataccgtct gaaaaatact ttgcgtctgg acgctggcag
    44221 gcaacgataa ggaaatatcc tgcttgtcgt cccaacataa cgattttctt taactggcta
    44281 agcaggctca cgctttcttt cgtcccaagc atttcaaaaa atgctacata ttcatcaaag
    44341 ataagaaagc agggtggaag tcccagatag gcgtagtttt cgccagtctt atagttcggg
    44401 tgtcgcttca tttcctcact tcgctgtacc atgccctcat aaaaggcatt gacgcaatct
    44461 atcatttctt ctttggtgtg atacacattt cccataactg tccctaagtc tgctaagtcc
    44521 gcatttttcg ggtctaagat gtaaaggaca gcgttggtat gcagtaaggc ttcaatgagt
    44581 gtcagcaaaa agtatgtttt accgccacca gttccaccag cgataagggc gtgagggagt
    44641 gcgtcatatt cccacacaag atttttcatc agtctaagac agccgttttc tgcccgtact
    44701 tcatcaatgg taatgcggtt tgctatcata tcataaagca gggtatattc gatatagccg
    44761 tcatgtaagg tcttatcggt cagttcacaa tacaagccac tttccaactt atcctctaac
    44821 cgtaaaagct ggtcttgata ttttcccaac gtgatttcac agcggatatg gagcagtccc
    44881 ttttccattt gataataaat cttcggaaac cagacgattt tttcccttga tctgctttgc
    44941 aggtcagtaa aaaaaccgct gtcttgtacg gtatcggctt cataccactt attttccagt
    45001 accattcgtg ccaacttttg acggtgcagg agcttcttga aactgtcgta acagaagcgg
    45061 taatacagaa aagcaaccaa tgcacaaaca ccagtcgcta tcagtatggt tatgaagttg
    45121 taaggggaga gcgtcaagcc gttttctaac aagctgaaat gctcccaatc ggtacgcatg
    45181 agctgtttga tattcaatag gaaaagaacc gccacgaata caaacagaag tgaccctata
    45241 aaaaagtggt aaacaaggtg tttgtcgctg gctctgatac ggtgtccttt attccaaatc
    45301 ttacgcataa atcaatcact cctttctggg tacgaaaaaa gcagtcaatc ataagactaa
    45361 ctgctataaa aagttatttt cacaaatcaa acacaatgca aatattaagc atttcattgt
    45421 tatactcggc tgaaattgta ccattcatct tttcaactaa tgcttttgaa atagacaatc
    45481 ccagtccagt tgaatttcta gcattattaa ctgtataaaa tcggtcaaat aattttccaa
    45541 cttctatttc agtcaaattt gaagcatggt ttataaaatg aatttctcca ttttcaaata
    45601 atgtaacttc taaatcacca ttactgtact tgaccacatt attcaataaa tttgcaaata
    45661 ttcttgataa tgcggaacgg tcaagattac gaactatctt tttctgtgga atgataatct
    45721 taggagaaat ttgcttttct tttaaaattg tataaaatga agcgatactt tcctctaata
    45781 cattgttaat agtaataggt tcaattttta aatcttcttc tgaagttaaa atcacagaat
    45841 aacgaaataa ttcttctgtt agttgtttta gcatttctgt acgatttttt atgacagcaa
    45901 tatatctatt cactttttca cttttttctt cttgttcaag taaatccaaa tagccatata
    45961 ttgcggttag cggtgtgcgt aaatcgtgag aaacatttgt aacagcattt tttaattcta
    46021 tatcgccttt ttgaaatttt ctgcgttcag ctcggagctt tcgtaactgg atatttattt
    46081 catttgctaa atgacacata tatttatcgt tgcttgaaat atcaatcaga gtattactat
    46141 cggttatgag cttttcagcg aatgaacatt ctatttcttt tgcagatttt cgtaataaat
    46201 gtatcttgat aagtaacaag acaatcacta aaatcagtcc acttatgaaa agccataacc
    46261 atagcataat ttttcactcc tttactttaa atcttttttc ttgaaaatcc aaacacctgc
    46321 accagttgta agtataacga ttactaagga atatataggc aatcgtgtta aattaacaac
    46381 ttgcattgtt gaacattgaa ttgcttgacc gcctggattt acatcataaa tgaattgcac
    46441 aatttctcgt tttgttccgt ctaaatattt tggattttcc atttcttgag ctgtgttttc
    46501 tccattttct cctatcgaat aagctggaat agtttttgga gcgtccaaca ttctattaca
    46561 cattgcacca gcaaacaaaa gcccaaatga aaatagaata caagctactg caataatagc
    46621 tttattttga gagagcattg ctacaagtgt gaaaagagaa gcaaatgtaa ctgataaaac
    46681 caagacagtt attcctatca ttacaatcat tttcatatca gcaacaaaaa atccaagtaa
    46741 aggaattcca acgctgagat acggaacaaa aaacacaata cacattgtaa ttgcaataat
    46801 agaacaagtt accatatttg ataaataaat agtttctctt ttctgtccta cgataatttt
    46861 gtttctgatt gttccgtcat tatgttctgt accgataaaa agactacaaa atacagcaag
    46921 aataattcct atgaacaatg cacacatgaa aaatccattg tcaagattat tgatatatcc
    46981 agactgtttc atatccctat atctcaatag tgggctagct attccaatca caaacataaa
    47041 agctaatgaa cccctaaaac atttattttt ccttaatcga aagaaattag cagataataa
    47101 tttattcatg tcttgcaccc cctaccaagc taacaaaata actttctaaa ctttcatcat
    47161 gttcatgtat cgaaataact tcacaatctt ttttggataa ttctgtcgtt aattttgaaa
    47221 tattaacttt ttcaaaaata tccgcttctg tattagaaag tattttatat tcaatgttca
    47281 tagtgtctaa aacataagca agggtttttg tgcttgatac ttttaaatga gtacattttc
    47341 ggcatttact ttccaattca atagagctaa tctgttttat cattttacca ttgtcaataa
    47401 agccatagta tgttgccagt tttgatagct cgtctaatat atgactagaa atcaaaacgg
    47461 taattccatg ttcttgattg agcttcaaga tcaattccct aatttctata attccttgtg
    47521 ggtcaagtcc attgactggc tcatcaagaa cgagaaaatc tggcgaacct gcaagagcaa
    47581 ccgcaatacc tagtctttga cgcataccaa gagaaaaatt ttttgctttt ttatttcccg
    47641 tattttcaag accaactaat tttaatattt cggaaattcc atcatcagag ggaataccta
    47701 atactttgta ttgctccttt aaattttcat acgctgttaa atcaacataa attgacggtg
    47761 tttctacgat tgctcccatt ctgcgacgag attttgtaat gtccttatct atgtgctttt
    47821 tgccataaag aacatagtcg ccagatgtag gctcttgtaa accacatatc aaccgtataa
    47881 gagttgtttt gcctgctccg tttttgccga taagaccata aatcgaacct ttaggcacat
    47941 tcattgataa gccgttgagt gctttaaaat gcccgtagtt cttacataaa gaatttgttt
    48001 taagaacata ttccatgatt tttacctcct gttttctgat atagttattc tatcttgtaa
    48061 gagtaaagaa aacggtaaag aaaacagtaa agaaacagta aagatttatt ctgttctcat
    48121 tttaaatcca attccccaaa cagcttcaat ataatctttt ccacttatat cacgaagttt
    48181 ttttcttaaa ttactaatgt gcatttttaa tgagcttcca gtacagtctg gtgtgtcctc
    48241 actaattttt tctaaaagaa atgactttac aataacttgc ttcggatttt gtattaacaa
    48301 tttcaaaata gcatattccg ttctcgtcag ttttactttt ttgtcctcta tgaacacttc
    48361 ataagtaaat gtatccaatg taatatcttc aaattctaat gttctgttta atgagggtgt
    48421 tttaactcta agtgcaacag taattcttgc caataattct tttgtatcaa aaggcttagt
    48481 aacataatca actgcaccac caagtaaaag agatacctta tcttcaacat ctacttttgc
    48541 actcataaca ataatgggaa tatctgatat ttgtggtaat aattcttccc cagacaatcc
    48601 cggtaacatt aagtctagca aaatcaaatc tggttttgta tctgctaaaa ataataatgc
    48661 ttctgtacca gagtaagcat gggaaaccat atatctttca tgtactaaaa cttcgtccaa
    48721 cattttgttt atatgaatat catcatcaac aattaagata tgcttcattt tcatcactcc
    48781 attatatttg atatgaaata attataccgt tatgcagtat gctctacaat ataattttta
    48841 ctcggcaatc ctattttttc tgttgtccct gcggtgtagg gttctgtgga ttacctgcgt
    48901 tctgattgcc tttattcttc aacacaatat cctctgcctt tacataccag tccacatctg
    48961 cacctgtata ggtttttcgt gataccgtat cggctactgg attgacaagt tccacaactg
    49021 cattgtacgg aaattccctt aacggtactt ctggcggtac ggacacgggg ataattccac
    49081 catgcagact gcacttcaaa tcatagatac gctttttaag ctgggtactc ggtgtcccgt
    49141 cctcattctg caagaacaca tcacgcaccg ctgtaaattt taattctcca aatgttttct
    49201 ctttgtcaat aacaaacccg tttgataatc tcataaatgc ttcactcctt tacttttctt
    49261 caactgctac aatatcatca gcaagcaaca cataatcggt atgtcccata tccccgattg
    49321 cgtaacctct gccgtataac ttcggattga caagttttac tttctgttca tacttgaaat
    49381 gcttttcgcc agcctgcacg ggaatttcca ccacaacatt ttcccctctc tgcacatctg
    49441 aataaaggtt atagcttctt cttgcgaaaa atctgcggtt atctttatct ctgtcaaaaa
    49501 catggtcgct ttcgcctgca aattcaagag ttccaaaaga ctgtgccata tctggcacga
    49561 catatttcat ttccatattg ttttacctcg tttctttcta tttttgataa gttttttgat
    49621 tgtgatttct tattctggcg gttcgggaag taccagcaat cccaccgata gtaaagggta
    49681 aaatcaatgc ttacgcaccc ttgactatcc gtgttcttgc aggttgttgg cagacaagcc
    49741 agaataagcg gaagtctgcc gtttgacagc ttcggcggag agcgtgattt tccttttctc
    49801 ataccattac cgccttatga tttcttcatt ttacggcgtt tgtagaagaa gatacctgcc
    49861 agtccagcac cagaagttaa aagaagtaca agcagttcgt aaaggttggt gttatcgccc
    49921 gttttgggac tgtcgctagg tctgctaggc ttttctggtg tcggtggtgt ttcggttttc
    49981 tctggttctt ctggcttttc tttaaaggta atcgtctgtc ccttatcctc aatatcctta
    50041 tgctcggtaa ctttcttcgg ttcgtctgga ttgcttaaat cgtataattc ctcaaaagtt
    50101 acaagctgtt taccgtcaag agaagtagcg tcaaaggtaa agataacttc cactttcata
    50161 gtttcgctgt cagcagtaaa agtgtaatca ctttccacac gctttccatt gataagaagt
    50221 tctgcatttt cttctttcaa catctgccag cccacaagtt tgtactgtgt accgacttct
    50281 aagccctcta aggttacggt atcaatgatt gtaacgtctt ttccagcttc aagctctttg
    50341 ttgccgtcct tatccgtagc agtcgtatga attttgatga tacgctctgt gataagtacg
    50401 gtctgcccgt cgtcctcaat gtctttgtgt tctgcaactt ttacgggttc gtctggattg
    50461 cttaaatcat acaattcttc aaaggtaaca aggtttttgc cacctaaagc agacgcattg
    50521 aacgtatagg aaatttccac tttcatttct tcctcatcag cgacaaaggt ataatcgttt
    50581 tctacacgtt tcccgtcaat gataagctcg gcgttttctt cctttaacat ctgccagcct
    50641 ttgagctgat attttgtgcc ttttgtaagt ccgtctaatt tgaccgtatc aacgattgta
    50701 acctcttttc ctgcaaggat agtcttttcg ccgtccttgc tggtcgctgt ggtatggata
    50761 gagatttctt tttcgtattc atcagtcagc gttcctaaat caatcacaag attatttctt
    50821 gataccacga tttcaaaagg tgggataagt tcaaagcctt tgttactatc agagcgtaat
    50881 tcttcaatga tgtaggtatc ataaagtaat gcacccttgc tgtcgtctgg ttcagaagtc
    50941 ccaaaccaca caccgtcctc actggtcttt cctgcgtttg tattgtgctt atgagaagcc
    51001 cattcagcag aagtggagaa ttgcccgtta tcatcagtta ccactacatg attttcaccc
    51061 gtcgtcttgc ttgtgatcct aaagggaaca ttagcaagac gcttgtgtgt acctgcaccg
    51121 atttttacac cctcaatatc tccacgctta atctgattgt agatagaatg agcttcgtcg
    51181 gttaagtcca cgatttttcc attttctgtg attgtaaaat caatcggttt tgcaccgtca
    51241 gttaagtaac cgtcgggagc ttgtttctca acgatacgga aatttccgta aggcaaaagg
    51301 tcagcggaag tagaagcgat accctcaatg tccgtatgga ttgtttttac gacttcattt
    51361 ttcttgtata gcttgccctc aaccaaaaca gaattatcat ttaaggaaat gatgtcaaag
    51421 gcagtatctt tcaaggttgc acttccttgt ggtttggtat cgcccgtttc taaatctcgt
    51481 ttctgaattt tgacaccgcc acggataacc ttgtcagaaa cggaaaactg gttacttcca
    51541 gataatacgg caaggtcgcc gtcctcggta atctgtgtta catataagcc cttaatctgt
    51601 tcggacttat cgccagcctg catatatgca ccgtcaagta agtagccatt tggagctttc
    51661 gtttcttcaa cggttagtgt tccaagtgga aaaacggctt taccgtcttg catatagaag
    51721 ctgtcgccag acaccttgta tgcgtccgct aattttgtga tgtagtgggt tgtcccgtcg
    51781 ctgtctgttt cagcgattgt ctttgtaacc catgtacgag tagcttcggc agggagattg
    51841 tctttagtat agaagcctgc ataatacttc catgtaaatt ccgcacctgc tagagaagcg
    51901 tttccttgcg gattgtcttt ctgtgtttcc atgtcaatct tgaaaagctc aatcaaagtg
    51961 tccgttactt ttggcgtatc agatacgttc aatgtcgctg tctttccagc ttcaatcttt
    52021 aaggaatata cagttttatc tactttatat cctgctggtg cggataattc cttgatatag
    52081 actgtgcctg cttttacctc tacaacatct gtatttccgt tttcatcagt cgtaagggtg
    52141 gcaagctgtt tcgtgcagtc cttatcagaa aagacaccgt agattgcacc agcgacagag
    52201 taattcccgt taccgtctgt aatgctggta ttactggaag ccttttgaag tttcgcattt
    52261 ccgacattca gtttcgccca gaattgtccc aattcctgcc cctcgccaga gtagatataa
    52321 ccgccacatt catagcgtcc tttattttct ttgacaaagg ctctagcacc agagaaaact
    52381 tcgtcctgcg ttgcctttgg aatttcatca taagaagctc gcacgttatc acattgccag
    52441 ccaagatgta cgctcaatct ctgccagact acaagctgtc ttaaaaggta agcgtgattt
    52501 ttgcttattc cgctgtggct gtctgtgtac tgtttcacat attcgataga taaggcaacg
    52561 tcgcttatct ggtcggcact catacgggtg cttgcgtcag ctctggtctt gtagccgttc
    52621 ctaaaatctg tgttaatgtc gatacagtaa gcgtcctcgc cctcaacggt taaatgtccc
    52681 tcattaaagg tagaaccaat cgaaccgtca ttcattactt tttcaacgat accgacacgc
    52741 tcttttgact ccgtccagta ttgcttgctt tctgcatgaa cgggtgtcgt cggtaaagca
    52801 gtaacgacag ttgcaagagc taagaagccc gtacacaatc gttttaacat ctttttcata
    52861 aatctaatgc tcctttcatt ttgggtaata aaatagccgt ccaataagaa cggctggaaa
    52921 tagaaaagga acgtcatgtt aggcgttcca tagtctatag gttattcaat tttttcttgt
    52981 ttcaatactg atgtgttgta ccatatcttt gcatatctag gaaaacttgc gtatcaaggc
    53041 tcattcgtta gtagtaaata agtagtaaat tgatgtgttt gtttccttga aaatgctgat
    53101 agatactgta ttttagcgga taatcagatt tttattgtgt ttttaataat aaaaactgtc
    53161 tttttaaaaa attcttataa aagacagttt tatcaatgtt atactttaat gtttaattaa
    53221 agccatattt tatttaaata tacaactatc tatcttagta catagtataa gatttttctt
    53281 tatctaatag tatatcctct acatctgggt tttcttttac taattgtcta taaaaattac
    53341 aattagtaac acccacatat ggaattaacc atagtagacc tattccaaga gtagctattg
    53401 aaagtatata ccatcctata aaactaaagt ttagtacaaa taatttaagt acatttcctt
    53461 tcatcatatc taaactagca cccattgatt taaatacacc catattatcc ttgtcttcac
    53521 aaattaagaa agtagcttga gaaacatata atagcaatat ataaactggt aacataagaa
    53581 caagtattat aagaactaga gttattccag tagaaataat agtccctatt ccaacattat
    53641 cacttgataa atattcaatt gctccactag taagtaaaac tccaaatgat aaaaacgctc
    53701 ctataattgt gattataaaa gaataaatat aaacaataat tgattcaaat aaataaaaac
    53761 caaaacttct taaataaatt ctcccactta caaagatatc tcctatcttg actttttcat
    53821 ctttagtaat tttcaagaac atacatgtaa atcctgctga aacgcaaaaa gctattacag
    53881 ccactacaat agtagtcaat ggttcaaaag gtaatcctac aattgatatt aatactatca
    53941 atgcaatata tactgccata gaaattgcac atatccccca gtttccagat aactgagctt
    54001 tagatactcg ttttaattct tgccttgata ccataaaaaa cacccctttc attttataaa
    54061 aaaactttta aaaatataaa taatctttta acgtataata ttaatatatc atattttcga
    54121 ctatataaga tacaattatt caccatgttt tatcaagata tgccataatt gtattactag
    54181 ttttatatag tcaacattac caaatacatc aaataaattt gattaattat tcaacaagta
    54241 ataaaataat tt
//
LOCUS       DAELNY010000006        49828 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.006, whole genome shotgun sequence.
ACCESSION   DAELNY010000006 DAELNY010000000
VERSION     DAELNY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.006"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(517..1326)
                     /locus_tag="KPE55_000321"
     CDS             complement(517..1326)
                     /locus_tag="KPE55_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0491423.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1410..1943)
                     /locus_tag="KPE55_000322"
     CDS             complement(1410..1943)
                     /locus_tag="KPE55_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0491424.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(2093..3442)
                     /locus_tag="KPE55_000323"
     CDS             complement(2093..3442)
                     /locus_tag="KPE55_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0491425.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3454..4836)
                     /locus_tag="KPE55_000324"
     CDS             complement(3454..4836)
                     /locus_tag="KPE55_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0491426.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMIGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            5080..6294
                     /locus_tag="KPE55_000325"
     CDS             5080..6294
                     /locus_tag="KPE55_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0491427.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6471..8384
                     /locus_tag="KPE55_000326"
     CDS             6471..8384
                     /locus_tag="KPE55_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0491428.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            8655..9629
                     /locus_tag="KPE55_000327"
     CDS             8655..9629
                     /locus_tag="KPE55_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0491429.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9650..9925
                     /locus_tag="KPE55_000328"
     CDS             9650..9925
                     /locus_tag="KPE55_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0491430.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9938..11101
                     /locus_tag="KPE55_000329"
     CDS             9938..11101
                     /locus_tag="KPE55_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0491431.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11438..13717
                     /locus_tag="KPE55_000330"
     CDS             11438..13717
                     /locus_tag="KPE55_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0491432.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSTGDTYFEINDSEKEVNKYLSKKLQLFEFDWLG
                     IIDSNGNFISALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYKGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSIYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(13812..15152)
                     /locus_tag="KPE55_000331"
     CDS             complement(13812..15152)
                     /locus_tag="KPE55_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0491433.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(15164..15946)
                     /locus_tag="KPE55_000332"
     CDS             complement(15164..15946)
                     /locus_tag="KPE55_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0491434.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15961..16983)
                     /locus_tag="KPE55_000333"
     CDS             complement(15961..16983)
                     /locus_tag="KPE55_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0491435.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16989..18002)
                     /locus_tag="KPE55_000334"
     CDS             complement(16989..18002)
                     /locus_tag="KPE55_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0491436.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(18130..19068)
                     /locus_tag="KPE55_000335"
     CDS             complement(18130..19068)
                     /locus_tag="KPE55_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0491437.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            19338..19802
                     /locus_tag="KPE55_000336"
     CDS             19338..19802
                     /locus_tag="KPE55_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491438.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19914..20231)
                     /locus_tag="KPE55_000337"
     CDS             complement(19914..20231)
                     /locus_tag="KPE55_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0491439.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(20244..20975)
                     /gene="chbG"
                     /locus_tag="KPE55_000338"
     CDS             complement(20244..20975)
                     /gene="chbG"
                     /locus_tag="KPE55_000338"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG0491440.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(21001..22311)
                     /locus_tag="KPE55_000339"
     CDS             complement(21001..22311)
                     /locus_tag="KPE55_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0491441.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22408..23721)
                     /locus_tag="KPE55_000340"
     CDS             complement(22408..23721)
                     /locus_tag="KPE55_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0491442.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23743..24054)
                     /locus_tag="KPE55_000341"
     CDS             complement(23743..24054)
                     /locus_tag="KPE55_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0491443.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(24254..24958)
                     /locus_tag="KPE55_000342"
     CDS             complement(24254..24958)
                     /locus_tag="KPE55_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0491444.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(25049..26410)
                     /locus_tag="KPE55_000343"
     CDS             complement(25049..26410)
                     /locus_tag="KPE55_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0491445.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            26909..28063
                     /locus_tag="KPE55_000344"
     CDS             26909..28063
                     /locus_tag="KPE55_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0491446.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(28145..29863)
                     /locus_tag="KPE55_000345"
     CDS             complement(28145..29863)
                     /locus_tag="KPE55_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0491447.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(29868..30857)
                     /locus_tag="KPE55_000346"
     CDS             complement(29868..30857)
                     /locus_tag="KPE55_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0491448.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(30953..32002)
                     /locus_tag="KPE55_000347"
     CDS             complement(30953..32002)
                     /locus_tag="KPE55_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0491449.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(32279..32947)
                     /locus_tag="KPE55_000348"
     CDS             complement(32279..32947)
                     /locus_tag="KPE55_000348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG0491450.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(32953..34326)
                     /locus_tag="KPE55_000349"
     CDS             complement(32953..34326)
                     /locus_tag="KPE55_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG0491451.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(34323..36101)
                     /locus_tag="KPE55_000350"
     CDS             complement(34323..36101)
                     /locus_tag="KPE55_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG0491452.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(36218..36541)
                     /locus_tag="KPE55_000351"
     CDS             complement(36218..36541)
                     /locus_tag="KPE55_000351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG0491453.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(36528..37505)
                     /locus_tag="KPE55_000352"
     CDS             complement(36528..37505)
                     /locus_tag="KPE55_000352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG0491454.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(37520..38083)
                     /locus_tag="KPE55_000353"
     CDS             complement(37520..38083)
                     /locus_tag="KPE55_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG0491455.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(38135..38626)
                     /locus_tag="KPE55_000354"
     CDS             complement(38135..38626)
                     /locus_tag="KPE55_000354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG0491456.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(38629..40554)
                     /locus_tag="KPE55_000355"
     CDS             complement(38629..40554)
                     /locus_tag="KPE55_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG0491457.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(40556..40867)
                     /locus_tag="KPE55_000356"
     CDS             complement(40556..40867)
                     /locus_tag="KPE55_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491458.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(41497..41772)
                     /locus_tag="KPE55_000357"
     CDS             complement(41497..41772)
                     /locus_tag="KPE55_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG0491459.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(41887..43296)
                     /locus_tag="KPE55_000358"
     CDS             complement(41887..43296)
                     /locus_tag="KPE55_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG0491460.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(43667..44329)
                     /locus_tag="KPE55_000359"
     CDS             complement(43667..44329)
                     /locus_tag="KPE55_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG0491461.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(44583..46802)
                     /locus_tag="KPE55_000360"
     CDS             complement(44583..46802)
                     /locus_tag="KPE55_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0491462.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(47078..49732)
                     /gene="adhE"
                     /locus_tag="KPE55_000361"
                     /gene_synonym="adhC"
     CDS             complement(47078..49732)
                     /gene="adhE"
                     /locus_tag="KPE55_000361"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0491463.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        16682 a         8619 c         6131 g        18396 t
ORIGIN      
        1 agttatctat attacaaaat tctccaaata tattttccaa ttgttttgat atatctatat
       61 ttattttgtc atcagttgat acaaataaaa tattatgttt tttcatagtc ccacctcctg
      121 tttaacgagt atagaaagta ataatttggt ctattgtagc actttttaaa aaagttttaa
      181 atcttaaaaa tcaaattttg taccatttac tattattttc cataataaaa taatgtaagc
      241 tatctttttt cacatataat ttaatttcta aaacggttag tttgacatta ttggaatata
      301 aacaaaaatt taatataaat tcgttaatat attaatacat ttttaagaat gacaaggaat
      361 aaattataac aaattttagg aatatataat aatagggtct cagaacttag ttacaagttc
      421 taaaacccta tttgtttagt gtaaaaaaca tcataaaatt tagcttaaat cttttcacat
      481 aatattactc tttttctaag tgataataaa atatatctat tcctctaaaa actgtaaaaa
      541 ttcctgatta aatagtttaa gctcatcata gaatatagca tgaccactat actcaaaagt
      601 atataaaatt gaatcttcta ttttttcatt cataaaaact gcaaattcat acggacatat
      661 ttcatctaat ttcccatgaa atataccagt aggcactctt acacatttta aatcattaaa
      721 taattcttca tctcttaaag atacagcagt tccaatagtg ccaatacctg atgcactaaa
      781 accaatatca ttaaaccatc ttctaaagct ttctggtgga tttgatgcaa atactttttc
      841 gccaaaatcg gttaccattt ctggtctatc agtacatgct tgtgctatca atttatttac
      901 atcttcacgt gtcataccat atggaaattc tggaggtctt tgtacaaatg atggtgcagc
      961 agctgcagct aataccaatt ttgacactcc atatccatta aataaactca tatacctaag
     1021 tacgattgct cctcccattg aaaaaccaac aagggtaaag tcttttaatc caatagcatg
     1081 taccacctta taaatatcgt ctgctaattg gctataagta tatcctcctg atgttgcatc
     1141 agatttacca aaacctctta aatcaattga aactgttcta tatccgagtt ttggcaatat
     1201 atttgtttga tattcaaata tcttatgtcc taagggccaa ccatgtataa aaagtacagt
     1261 ttttttagca ctcggattca aatcatatac tgctatgttt atatcattca catatacata
     1321 atacatatca acagcaactc ccttcaagaa aatgtatgat ggttatttta tatatatgaa
     1381 taaaatgatg aataactaaa ttttcaaatt tatttacaat acttctctat tgccttttca
     1441 aataaactta catgaagttt ttcatctagg attattctat ttaatatagc ttttatataa
     1501 gggtcttcaa taagacttat gtctttataa taattattaa tagccacatg ctccaaatgc
     1561 aaatctagtt ttaatctgtt acaaatagaa tttccataac atacaaaacc accattccaa
     1621 tatgcaccac aagaacttgt agaacctcta tatacaggat ttccacccaa taatcttata
     1681 gtctttgcta atatctccat atgtaacatt tctgttatag atatatttat ccacatttca
     1741 cttaactctc tatgcgtttc atctaaatca aaattatggt atagatattg atttacactc
     1801 gtaaattcac ttgatactcc ggcatagtca tccatcaata attctgcata gtacttattt
     1861 ggacctaaaa cttttatctc tggatatggc tcatcacttg aatatccatt tcgcttgtgc
     1921 atgtcataat gtgcatcact cataatatct cctcctttaa atttaatgta tgatattaat
     1981 tgtatgcatc acaacttttt catataactt agataataaa atctacttaa ataataaaaa
     2041 gccatattaa ttaaaattaa catgaccttt tatccacaaa attacttttt aattattcat
     2101 cttttccagc tactgatagc tctttaataa caactgaagg tgaacctata ctactcattg
     2161 gaaattctaa atcttctcct atagcttcaa tatctttcaa caggtcaaaa tagtttcctg
     2221 ctactgtaat ttgctccact ggaaatactt tttttccttc tttgatataa aagcctttgg
     2281 cagctaaaga aaagtctcct gttactgaat ttgcacctga atgaagtcct gcaaagctag
     2341 taaccatcaa tccttcatct atatccttta taatttcatc taaagattta tctcctttct
     2401 ctatataaaa attagttgga tatatgctta taggtgatga atatgaagat tttattccat
     2461 ttcctgtagt ctttacacct gccttattag ccgtttttaa gttatgaagt aaagttataa
     2521 gctttccatt taaaacaact tcttttttaa atgtagctac tccttcatca tcaaaaggag
     2581 tagatgccat accattttct aaaagtgggt catcaactat tgtaactata ggagatgcta
     2641 tcatgtctcc ttctctattt ttcaataaag ataatccttt ttgagctgaa tctgcattaa
     2701 atatcccaga aaatgtgcta agcaaagata ccatagcttc attaagcaat atagttttat
     2761 atttacctga tggaatactc ttaccaccta ctttagataa agcattatca acaccaagtt
     2821 ttgctatctc acaaggtttt atttcttcaa tagaatttgc tatattatat cctattccat
     2881 catatttttg tccattgtct tctatgatag gaactacaaa ccctataagc atattagttt
     2941 tattgcttaa attaagacct ttagtattag atataccatt acttgataca gaataagata
     3001 tagtacattt agataaattt accaccttat ctgaataagc tttagtctct ctttccattt
     3061 ctaaagctaa atcaatcaac ttatctgcct ctaaattttc tagctcttta gaataagtct
     3121 ttacatcatt atagtgctta tctccttcat atataaattg tacatcttca ttttcaatag
     3181 tgtttactcc atcttttaca ttttttatca acatatcaac agctttatca tctaaaattt
     3241 ctgtataaga ataacctatc tttccattta ccttacctct aaaagataaa ccaaaagatt
     3301 tatcaagatt gtatttctca acttctcctt catatatatt tatacttaaa ttttctcctg
     3361 tataataata tatttcacac tcttcaaaac cttcacttag agccttttta aataatatat
     3421 ctttaaaatt tttaagttcc acaattatcc ctcctatctt ccacctacag taatttcttt
     3481 tacccttatc ataggttggc ctacattagt tggtatactt cctgatgatg agccacacat
     3541 cccctgagct tgttttacgt tattcccaat catatctata ttcataagta tatcgctacc
     3601 ttttccaatt aaactagcac ctctaactgg ctcttgtatt tctccatttt ttattaagta
     3661 accttctgaa actgcaaaat taaactctcc tgttacagga tttacagaac caccacccat
     3721 ttttttagca taaagtccat ctggtattga ctttataata tcttctggtt tatcctctcc
     3781 agcagcaata taagtgttag tcatccttga agttggtgca aatttgtagt tctgtctacg
     3841 agaacttcct gtaggattca ttcccattcg tctaccatta agtttatcta tcatataaga
     3901 cttcaaaatt ccattttcaa tcagtatatt cttttgagtt ggatttcctt catcatctat
     3961 attcatagaa ccccagtagt ttggtattgt accatcatct atagctgtaa tctttgtgga
     4021 agcaatctgt tgacctaatt tatctgcaaa tacagagttc ccctttgcaa ctgatgtagc
     4081 ttccaaagaa tgtccacaag cttcatgaaa tattactcca ccaaatccat tatctattgc
     4141 taccatcata ttacctgcag gacaattttt agcatgcaac atagtatgtg ctactctagc
     4201 tgattctttt gcataatatt ctgggtctat ttcttcaaat atttcaaacc ctttacaacc
     4261 tccaggacct tcaaatccag tttgattttc attaccctta gatgcaattg aacttatccc
     4321 tagtctagtt cttattcttc tatcctcaac atatatacct tcagtgtttg caattaatat
     4381 tttttggtct ttatccaaat aactaacact tacttgagat atatcattac taaattcttt
     4441 tgcactctta tatgcaacct tcataacact tactttatct ttatttccta ttgaatttgg
     4501 ataaattttt atattatgta tgttatttat tttaatactg tcatttaata ttacactttt
     4561 tccatcttcc aatttaccta aagcaacagc agctttgtat gctacatcca acagactatt
     4621 taatgaattg tcacttgtat aagcatatat acttttcaat cctttaaata ttcttattcc
     4681 tataccataa tttcttccac ctattgcatt ttctacttta ttatctatta aactaataga
     4741 attatttata gtgtcttctt caaaaatctc agcaaaatct cctccagtaa ttagacactt
     4801 ttctaatacc ttagaagcta tattttttga aagcataaat ttacctccta ctttaaattt
     4861 ttaattaact tttattcaat ctttttactt gttaacaaac attatatact atattactta
     4921 aaataaggta attatgaata tattatacat atgttttata ctaaaatgtt atcacatttt
     4981 atctatacat acccatataa ctagataatt aaaatgtata atattatttg ctatagaaaa
     5041 taatacactc atacataata aattttagga gtgaagtata tgaatcaaga atatgtaaaa
     5101 ggaaatccta tttttactaa tattaataaa gttccaagac aatttccata ccttactgat
     5161 gatattgata ctgatgttat aattgttggt ggtggcgtaa ctggatgtat ctgtgcttac
     5221 tatctagcta aaaacaatat aaagtctgtc attcttgaaa aaggaaggat tgcacatgga
     5281 agcactagcg tgacaacttc tctattacag tatgaattag atgacaattt gatagattta
     5341 acagaggtta tgactctaaa agatgctcta aaagcatata acctttgtac ttcagcactt
     5401 gaagaattag atacatttat agagctatat ggaaataagt gtgattatgc caaaagagat
     5461 actctacttt atactgccaa caaacttgaa gttaaggcta tcaaagaaga atataactta
     5521 agaaaagaaa atggatttga tgttgaatat attgatgaat ccacaaaccc ttttagcttt
     5581 gatttaaaat caggattgat agcaaagaat ggaggacgag aattagaccc atataaatat
     5641 agtcaccatt tgattgatgt aagtttaaaa aatggtcttc aagtatatga aaatacagaa
     5701 gtaaaaaaag tagatttatc aaatgataag gttactgctg aagtatcgta tggatacaaa
     5761 gttcatggta aaaaattaat tgtagctaca gggtacaata caagtttatt tacaaaaaga
     5821 aattttgcaa ctaagtcaaa tacttttaat atcgcaacga aaccacttaa aaacatagct
     5881 tcttggaaaa acaacatttt aattagagat aactgtgacc catataatta tctaagaact
     5941 actaaagaca atcgattaat tataggtggt gaagatgtaa gttttgatga tatagaaaat
     6001 gaaactttag ccaatgaaaa gtacgatatt ttagaacaaa gattgaaaag tatgtttaag
     6061 gatataaaag acattgaagt tgaatataaa tattgtggtt gttttgcttc aactttagat
     6121 aatttaggtt ttattggtcc agataataaa aataacaatc tatggtattg cttaggttat
     6181 ggagcaaatg gtatactctt tgcaatattg ggaggtataa tgttaagtga attatatctt
     6241 ggcaaacaaa ataaaaatat gaaattattt aaagtagata gatttgataa atagatatta
     6301 tatattaaaa gactgtaaaa agatgaaact ttataattct aaaagttaac tttgatacag
     6361 tcttttataa tgttcctttt aaaaatactt tatttataat taaatataaa gtaaaatgtt
     6421 aaaatataac taataaaatt tagtcataag ataatattta agtttatttg atgaaatatg
     6481 gaggaattat tatgaaggaa atactcgtta ttgctcccac taaaggaacc tatgaaaaat
     6541 caattcacat agttaaaaaa aacaaatata caaatattga tgttgtattt ggcaatctaa
     6601 aagaaggtat acctcttgct gaaaaaagta ttaatcatgg tactagaatt atcataagcc
     6661 gaggtggaac ttacaatatg ttaaaagcta cttacaatat acctatagtt gaaattaagg
     6721 tagatgctta tgatattata aaaagctaca aagaggtaaa aaattccaac gaaccatttg
     6781 gaataattgg atttaataat gtcatctacg gtttcgatat tatagaagaa atattgaata
     6841 agaaaataac tatgattgaa attgaaaaag aagaagaaat ttatgatgct atagaaaagt
     6901 acagaaagaa aggtataaac acatatatag gagataccac tgttgcacat attgttaaga
     6961 gacttaattg taagggaata ttaatcgaat ctagagaaga aaacatactt agagccattc
     7021 aacaagcaga gcaaatactc gaagcaacca aggatgaaca aaaaagaaga ttacagatag
     7081 aagctatgac agattttgta catgatggaa taataacagt tgataaaaat tttaaaataa
     7141 cattatttaa taagagtgct gaaaaaatat ttggtataaa aaaagaaaat gcactttatc
     7201 ataatgttgt agatgttatc ccaaacacag ttcttccaca agtagttgag actatggaac
     7261 ctcaaattgg tgaaatacaa aatataggga gtacaaaaat tattacaaat agagttccaa
     7321 tcattgttga tggtcagatt caaggagctg tttcaacatt tcaagatagc aaggagataa
     7381 gttcttttga acatacaata agaaggagct tattaagcaa aggtctatct gctaaataca
     7441 cttttgatga tataattcat gctagtgaaa agacaaaaga ttgtatagaa cttgcaaaaa
     7501 aatatgcttg ttatgatact cctatgatta ttactggaga atctggagtt ggtaaagaac
     7561 tattttgtca aagtatacat aactatagta agcgtaaaaa tgcacctttt gttgctgtaa
     7621 attgtgctgc catacctcca tctctaatag agagtgagtt ttttggttat gaggaaggtt
     7681 catttacagg tgctagaaaa aaaggtaaag caggtgtatt cgaactagca catgaaggca
     7741 caatcttcct agatgaaata agtgaaatcc ctcttgaact tcaaggtcga ttattgaggg
     7801 tattgcagga aaaacaagtt atgagaattg gtggtgataa agttattcct atagatgtaa
     7861 aaattatctg tgcttcaaat aaagacttaa aaaatatgat gcgcaatggt ttatttagac
     7921 aagaccttta ttttagaata aatatactaa cattatatct tccttcatta aaagaaagaa
     7981 aagaagatat tataaaatta tcagaatttt ttatacataa atattcaagc aaatacaata
     8041 aaacgccttt aattatcact ccaaatatta aaaaggcact tcttgaacga gaatatgaag
     8101 gtaatataag ggaattggaa ggtatgatgg aaagggctgt tattgtggaa tcatttgact
     8161 cgattttatt agaaaagctt agcaatgaat atacaaataa tatagttgat aaagataata
     8221 gtgattttga atgcagtaat ttagatttaa aaactttaga aaaaaaatat atctataaaa
     8281 tttataacga aaataataga aatactacaa aaacctgtga aattctaaag ataactcgct
     8341 ccactctatg gcgtaagtta aaagaaatag aagatgatgt ttaaaaataa tacatatcta
     8401 tatatatcaa aacgttttat gtttaatttt aatacataag acgtttattt gttttcaata
     8461 aaaaatacaa ttatatgata aaaagaatct ttgtaagtga aattctcgtc aatcatactt
     8521 ttaattataa gtaatctaca gcatttcaat cgattattaa aaataatcat aataactaaa
     8581 gatacaaact ctatatattg gcatagtaat tgcttttata atacctatat aaaaatgaaa
     8641 gggagtgata ctagatgcac atcttaaaat cagtgaaaaa gataccagga ggacttatgg
     8701 tctgtccctt gttatttgga gcactattta atacagtatg tccacaagct cttgaaattg
     8761 gaggattcac aacatcctta tttaaaactg gtgcaatgag tatacttgct ctattttgtc
     8821 tatgcaatgg agctcaaatc aatatcagac aagctggtaa accattgact aaaggtatag
     8881 tacttacagc aacaaaattt ataattggtg cagtattagg tattattgtt agtaaattta
     8941 ttggacctaa aggtataatt ggtattacac ctcttgccat agttgctacc atgactaatt
     9001 caaatggagg tctatttgct gctcttgcag gtgaatatgg agactccaca gatgttggag
     9061 caatatctat cttatctcta aatgatggtc ctttttttac aatgcttgct tttggagtaa
     9121 ctggacttgc aaatgtacct atagtagcac tatttgcagc acttatacca atactacttg
     9181 gatttatact tggtaacctt gatgaagata tacgagaatt tcttgcacct ggtacaacat
     9241 tattaattcc tttcttcgct tttcctttag gagcagcatt aaactttaat caaattataa
     9301 ctgctggact acctggagtt gtacttggtt taggagttac tataataaca ggtatgggtg
     9361 ggtactttgt aatgaaactt atacgtgcag aacatccagc agtaggtgca gctacaggta
     9421 caacagccgg aaatgcagct ggtactcctg aagctttagc agctatagac cctactctag
     9481 ctacaatagc agctgtttca acagtacaag ttgctgctgc tattattgtt acagcaatat
     9541 gttgtccaat gttagtttcc tatctagata aaagagaaaa aaagaaaaaa tctattttta
     9601 aaacaaataa aaatattgaa gtaaattaat acttaaggag gtaaaattaa tgaatgcaat
     9661 tattcatgca aatgaaaaag ataatctagt tacatgtgtc agacctttaa ttaaaggtga
     9721 aaaagtttca attgatggaa agtcttacat agtaaatgat aatatacctg tctttcataa
     9781 gatggctaca gaagatgtaa aaaaaggtga tgttgtatac aaatacggtg aaattatagg
     9841 aattgcaaca gttgacataa aaactggtga ctatgttcat gtacataaca ttgaaagtac
     9901 acgtggtcgt ggagataaaa aataggagga agttaaaatg aaattaatgg gttatttaag
     9961 agaaaatggt cagtttggta ttcgtaatca tgttttaatt attccaacat ctgtatgttc
    10021 aagtgagaca gctacaagaa tagcatcact tgtgcctgga gcaatagcaa tccctcatca
    10081 acatggatgt tgtcaaattg gttctgatat agaacttaca gcaaagacat taataggatt
    10141 tggaaaaaat cctaatgttg ccgcagtttt agtagttggt cttggatgtg atggtattca
    10201 agcaaaagaa ttagcatctg aaatagctac aacaggaaaa aaggtagatt acgttgttat
    10261 acaagaatgt ggtggaacat taaaaacagt ttctaaggga gctgaaatag taagtaaaat
    10321 ggctcgtgaa gtatcaaaag aagtaagagt tgaatttggt atgagtgaaa ttacattagc
    10381 acttgaatgt ggtggttctg acccaacaag tggaatagct tcaaaccctt caataggcgt
    10441 tgcatcaaac cttcttgtag atgaaggtgg aagtagcata ctaagtgaaa ctacagaagt
    10501 aattggtgca gaacatttgt tggctacccg ttttgaagat gaagaaatga aagataagtt
    10561 tcttaaattt gtaagtgatg tagaaaaaag agcaattgct atgggagaag atttacgtag
    10621 tggtcaacct actccaggca ataaagctgg tggactttca acaattgaag aaaagtcact
    10681 tgggtgtatg tataaggcag gcaataagcc attcaaaggt gctcttgaat atgctgatat
    10741 tgtacctcct gataaaaaag gattatattt tatggatact ccaggtcaag atatagattc
    10801 tatcactggt atggtagcag gtggtgcaca aattgtaatc ttttcaactg gtagaggtac
    10861 tccaacagga agccctattt caccagttat taaaatcact gggaatagtg atacatacaa
    10921 caaaatgcca gacaatatcg atataaatgc aggtagaatt ataacagatg gtgctaaaat
    10981 agcagatata ggtcaagaaa tatttaatga aataatagaa gtctgtaatg gtaaacatac
    11041 caaagctgaa agtcttggtc atagagaatt tggcatttac agaatttcat ctacatttta
    11101 gattgtaaca aaatatcttt tataaatata atacttatgt agatacataa gctaaaagtt
    11161 ctttctaatc ctataaaaat tttctatact gaaaaggaac tgtatcaaaa ttattttgaa
    11221 aatagttcct tttttcacct cttttactca aaatattata aaataagatt taaacaataa
    11281 acgtatcttg attaatctct aagaagacat aaataaagct aaatgtaaag tgagataaat
    11341 aaaatttaat atctttgtat gaaaagatat ttggtataat atgtctgaga gaattcttat
    11401 ttgtaaacaa ataaaggatg gaggatgaaa caaaagaatg aactttgaaa acacaaaaaa
    11461 taaaaaaata atagctactt tactattgac actcctttta atcgtgtcat taacatgtgt
    11521 cacttttatt atgaatctaa ataaagtaac acataatgaa acagaacgat atcttgtaga
    11581 agtttcacaa agtatttcca ctacaattaa tgtgaaatgt aaaagtatat ttaggacact
    11641 atattctacg ggagatactt attttgagat taatgatagt gaaaaagaag tcaataaata
    11701 cttatcaaaa aaattacaat tatttgaatt tgattggtta ggtattatag attcaaatgg
    11761 aaattttata agtgccctta aagatacagg taatgttaaa ggacaagact tttatagaaa
    11821 agcccttcaa ggtaaagaaa caattactcc tgttaaatca caatctggta catatggaat
    11881 tttatattct gtaccactat acaaaggtga taaaattgtt ggagctgttg ctgcttggaa
    11941 tactattgat actatgagaa atttattaag tgtagaaagc tttcatggtg aaggattttc
    12001 ccaaattatc gaccaagatg gaaattttat tgtaagttca gcaaacaaaa atgctccaaa
    12061 aaatgtggtg aatttttttg acatgattaa acaccaagga aaacaggttg ataaaaataa
    12121 acttgaagaa atgaaaataa acttaaaaaa gaacaaaact ggttctatat attacacatt
    12181 aaatgatggt atttctaagg caatgcacta tcttccatta caagaagaaa atttatacct
    12241 cctttcagtt gttccaacaa aaacagcaag tgcacagttt gatttacttc taaaaaaagc
    12301 aatcttcatc aatattttaa ttgttatact atttttattg cttattatat tgattatgtt
    12361 tataaatcaa aaaaacaggg cacaacttat aaaaatcgcc tatgtagact ctgtaactaa
    12421 aggttacagt aaagcacgtt ttgatatgga agctaaaaaa attatacagt catcttcatc
    12481 agaaagctat tcattagtat ctataaatat tcagaaattc aaacttatta atgattcttt
    12541 tggtagtgaa gcaggagata aaacattaaa gtacatacat gatgtcatta gtaattatct
    12601 agaaccagga gaattacttt gtcgagtttc taatgaccag tttaatgtac ttattaaaac
    12661 tattcctcaa aaagaaattc ttaagtacat agaaaatatc accaatgata ttaatagttt
    12721 caataaaaat gaagaatgta aatactttat agctctatct ataggtgtat ataaaattga
    12781 tgacccaaat ctacctttag tttcaattca agaccgtgca aatgttgcac gaaaaaataa
    12841 taaaggaaac attggaaatc atccacttta tacatgtgtt ttttatagtg atttagagcg
    12901 tttaaaaatg attaaagaaa aagaaatgga aaatagaatg gaggaagcat taaataataa
    12961 cgaatttata gtatatcttc agcctaaaat aagcctcaaa gataatacta ttgtaggtgc
    13021 agaagcatta gtaagatggc aagacccaaa taaaggtctt ataccaccag acgagtttat
    13081 tcctttcttt gaaaaaaatg gttttattac aaaactcgac cactatgttt ttgaaaaagt
    13141 atgcagtatg ataagaaaat ggatagataa tggagaaact ccaatacctg tttcagttaa
    13201 tttttcacgt gctaatttaa ataatataga ttttttagaa aaatacagaa tgcttcgtaa
    13261 taaatacaac attcctgcta agctcttaga aattgaatta actgaaacac ttgtatttga
    13321 aaatctccaa aaattaatta atataataga ccaaatccat gaagaaggtt ttcaatgttc
    13381 actagatgac tttggaagtg gatactcttc actcaatatg ttaaaagaaa taaaagttga
    13441 cacattaaaa cttgaccgtg ctttttttag ctcacctaat gcagataata ttgctgaaaa
    13501 tcatgtaata gaatctatag ttaatttggc aaaaaaatta aatatgaata gtatatcaga
    13561 aggtgttgaa acaatcttgc aaatggatta tcttaaaaaa attaattgtg atatgataca
    13621 aggatatgtt ttttcaaaac ctctacctca agaaatattt gagaaaatga cttttggaaa
    13681 aaaactagaa gaaaccagta tggatagttc cgtttaaata aaataaaaaa cttgtctcta
    13741 taaaataatt ttaatcattt tataaagaca agtcttttta ttttccaatt taattttatt
    13801 ttctaaattt cttacactcg gcttgtttta aattcccttt ttaagaataa taatacgatt
    13861 atcatagcaa taaaatctgc aactggctgt gccaaccaaa caccatctaa attaaattga
    13921 attggaagta caatgattaa tggtataaat aaaataaatt gtcttaaaag agttaaaaac
    13981 attgaatgtt taactttact tatacactga aaatacacag gtcctacaat agaaatcccc
    14041 attataggca atgtaattag atttaggctt atacctctta ctgctatatc catcaaatct
    14101 gaatctttat taaatatacc tacaaatact tctgggaaaa cttgtactac taaaaatcca
    14161 aatgacaata tgacaactga cactatgata gataatatta aggttttctt tgcacgctca
    14221 tattgttttg caccataatt gtatgatatt atagtttgca taccttgatt caatccaaac
    14281 acaggcatca taaacatcaa taaaatagat gtaagtgctg tcatagctcc aatagctaaa
    14341 tctccaccat aaacctttag agaattattt gtaaacatgt gagttatact tatagctact
    14401 tccatagcaa aaggtgtcat tcctattgca aataccttct ttactatatc tttattcaat
    14461 tttaagttac atttttttag ttttaaattg gacttccctc ttataaaata atgtattacc
    14521 caaaaaaata ctactatttg acagagtact gttgctatag ctgctccttt tatacctaaa
    14581 ttaaatacaa atataaatat agggtccaat atcaggttta atacacatcc tacaatcatt
    14641 gtccttgctg ctaattttgg attaccttct gccctaattg cattattaag agaaaatgca
    14701 actaaattaa atatacttcc aaccaatatg acactcatat agtcttttgc ataaaatatt
    14761 gaatctttac ttgcacccaa aataaaaagt atctcttcta agaaaactaa tcctaatata
    14821 gttataatta aagagataat tatagataat gtcaaggcat tacctaatat cttctctgct
    14881 tcttctctat ttctttcccc taactttata gacaatcttg tgctagcacc tactgatacc
    14941 aacattccaa aagctattat tagagtaaat actggcatag ttacaccaag acctgcaata
    15001 gctatagagc caataccttc catagaacca ataaaagctc tatctactac attataaagt
    15061 gaagttacca tcattgctat tattgcaggt atcgaatacc taaataataa aatccatatt
    15121 ttttcatgtc ttaatgcttc ttgattctcc atgttgtcac catttacacc atgtcataag
    15181 ttatacaaat tggtttattt gttcttgggt ctgttgctat ttctgcatca atattaaata
    15241 attcttttag attatccttc gtcataacat catatgcatc accttcacaa actattttac
    15301 ctttcttaat acctatcatg tggtcagcaa atcttgctgc attattaagt tcatgtatta
    15361 ccatgacaat agttcttcct tgttttttat ttagttcatc taataatttt agtatctcca
    15421 attgatgtgc caaatctaaa tatgttgtag gctcatccag taataatata tcagtttctt
    15481 gtgccaaagc catagctatc catgcccttt gtctttgtcc tcctgataaa tcttccatat
    15541 ttctatcctt aaattctgca attccagtaa cttctaaact ccaatttaca atctctctgt
    15601 cttctttact agaattacca aatccacttt tatgaggaaa tcttccataa gaaattaatt
    15661 ctgaaacggt aagaccacta ggagcttgag ggctttgtgg aagtacagcc atatcttttg
    15721 ctatttcttt agacttcttt ttgtagatgt cctctccatt tatatatatt ttcccactat
    15781 tagggctaat aattctacct atagttttaa gtattgtcga ttttccacaa ccattagccc
    15841 ctatgatggt agttattttt ccttttggta tatttaaatt taaatttttt ataatatctg
    15901 tgtttccata tgatacattt agattcttag tatttataca gttcataaca acaccctttc
    15961 ctaatcattt accaacatta aataaataaa gtacggcact cctattatag aaactactat
    16021 gcctactggt atttctatag gtgctagaag atttcttgat atagtatctg ctattaaaag
    16081 taataatgtt cctatcaatg ctgatactgg tattaatctt ttatgctttg gtcctattat
    16141 ttttctacct atatgtggag ctattaaacc taaaaatgaa atactacctg ctactgctgt
    16201 cgatacacca gataaaatta ctgcatatat tataagtctt cttctttgtt tttctacatc
    16261 tacaccaaga cttatagata cttcatcacc caaatttaaa acatctatat atttagattt
    16321 ataaatagtt atacccataa aaatcactat aaatggaagc acagctatta catatttcca
    16381 actggttccc caaatgcttc ctgatgtcca tgccataacc ctattaaatt cttgtgttgt
    16441 aaatttcaat tggaatatcg tcaaaattga agtaaaagct atgtttacac ctataccaat
    16501 aagtagtaat cttgacgaat ttataccacc tttccatgca agagaatata taagaaatgc
    16561 tccaaataaa gctcctataa gtgcaacaat aggcattgta aatacagtca agttactcat
    16621 accttcatat acattaccat tcataaggta tatataaact actacaaaca atgcagcacc
    16681 tgaatttatt cccaaaattc cagaatcagc taaatcattc tttgtaactc cttgaagtat
    16741 tacacctgct gttgataatg cagaaccaac caaaagtgct attactattc ttggtaatct
    16801 taatttaaat attgcaattt catgattacg ctgaccctgc cctaaaaaag tctttataac
    16861 atccattggt tctatagaaa atgagcccat atttaaactc aataaaaaag tgataaaaat
    16921 aagcaccatt aaaattgata taacaagtaa tgttttagaa tttattttta taatattttt
    16981 catagtacct atttctcccc ctttctaaca aggtatataa atattggcac tcctataagt
    17041 gctgttatag aacctactgg tgtctcatat ggaggattta tcatcctact taatatatct
    17101 gtatatacaa gcaatacagc tcctaatact aaagagctcg gtattatata cttataatct
    17161 gaacctacta ttcctttagc tatttgagga actataagac ctataaaaat aatattacca
    17221 gacactgaca cagatgctcc agtcattaat attacaagta ctatacatat aaatctaaca
    17281 aagttagttt tttgtcctaa tcctattgcc acttcttctc caagacttaa tatagttatt
    17341 cttggagcca taataatagc taaaataaat ccaatacctc cagctataaa tagcaatttt
    17401 ataccttccc acttcactcc tacaagccct cctgatatcc agaaactcaa ctgttgagat
    17461 aggttaaaat acatagatat tgctgatgcc aaagaaatta acaatgtacc aagagctact
    17521 cctgcaagtg ctagttttac attattaact cttcctcttg atttagaact tataaaatat
    17581 ataaataatc cacttatact tgccccaata aatgcaaaca tcattacact aaaacttcca
    17641 tgtataagtt gaggaagttt tttttgaagt accagtgata tcgaaattgc aaatgtagcc
    17701 ccttgtgtaa ttcctataac agatggttca gctataggat ttctcgttat tccttgcatt
    17761 attgccccgg aaactgctaa aaaacctcca acaagtgcag ctgctattgc ccttggaagt
    17821 ctaacatcct ttactattct catatttatt ccatcttcca tttttatcaa gctatctata
    17881 acagttgata gattcatatc ttttgcccca atagttatgg ataatacaat tccaaataca
    17941 agtacaacta tacttactaa tatcaacaaa taagttatat tttttttact taacttagtc
    18001 aattatccta cctccttttc ccttttaatc gtcagtataa tatcatctat taagtatttt
    18061 aattttaagc tttattaaaa ataggtgtga aaaatgaata tcattatctc acacctacta
    18121 agttaagaat tactttgtta attcattttt aactgattct aataataact cttttccaat
    18181 tggattgtat gattgactga aaaatggagc agcgtcaagt attgttacat tgccatcttt
    18241 tactgctctt atttgagccc agatagcact attttctaaa tcttttttat ctgattctgt
    18301 agcaattaca actatatggt ctgcatctat atctgttaaa ccttccatac ttaccattgg
    18361 tagtgttatt ccatcctgtt ttggcatatt cttaggtcta gctaatttca tatcatcatt
    18421 gattagagta cctattccac catcgctaaa taccataaat tgacctgaac ttgccaacac
    18481 tggtaaataa gttttttctc cattcttttc tataacttcc ttacctaact ttgtagcttt
    18541 ttcatcatac ttctgtagcc aacttttagc ttcttcttct ttgtcaaata actttgaaac
    18601 atcagttaac ttgcttctcc agtcatttgc ataatctttc atcataacta caggagctat
    18661 ttcttttaat tggtcatata ttttttcttg tctttgactc attattatta aatcaggatt
    18721 tacttctaat atagcttcca tatccatagt atccatcatt gagtgcccaa ctatctttac
    18781 acctttaagc tcttctctta tgtaacttgg taatttatca gtttcatatg agtctacatt
    18841 tgctgttgca acaggcttat aacctgctaa taatagttct tcactacttc ctgatatatc
    18901 aactattttt ttaggatttg atggtatctt aacctctcct ttaacagatt gtacaactct
    18961 tgtctctgat ttatctttat cctctgtatt cttatctgaa catgctgtaa caagtactaa
    19021 tgtagttata atagatataa atattgcaag tgattttatt tttttcatga ttacttctcc
    19081 tacttgttta ttatacttac atgatattga ttatcataag cacatattta ttatagcatt
    19141 tttctatttt tagtcaatat tattttgtaa aaggataaaa attcctaatt aatcttaatt
    19201 atatatagct tagataaggt tttcttatat taaacagtat ttttttaaag aattggacac
    19261 aaatacaatg acttttaggt taaacaatga taaaatgcaa tttatagata atgaatttag
    19321 taaggaggat tcaaaacatg gtacatttag tatattgtga taatgcagga aaaaaaggag
    19381 aaaaggtctt agataaaatc ttggctggaa caaaaacaat ggttattaga ggtgctgctg
    19441 gtagaaaaat accacatagt agagtatttg aagatgagtt actttatttt atggaaaaag
    19501 gaactgcaaa aatatctgca aaagccactg ttaaaaaggt agaaaactat gttaaattaa
    19561 cagaagatga aatttctaaa attttaaatg aaaatcaacc aaaattaaat ttatctgaaa
    19621 agcaaaaagt tcgttggcat aaaaaatgtt tatgcttagt tgaatttgaa aatgttgaag
    19681 aaataacacc tcttgatttt gaccatcaag gaaatatgga tgattggctt attgttgaaa
    19741 aaattgaaga tgtagttgtt ggtactagta taccttacaa ttatgagaag tctaaatttt
    19801 aaagtatcaa taacataata ctatattgct aaactctact taaataaaaa aagagtgcat
    19861 ttaataatta taaaaattat ttttatacac tcttttttaa taataatttt aaattactta
    19921 ctttctaatc tttcatatac atctataaat tcttctgcta attgttgaaa attcatagct
    19981 gtcattaaat ggtcttgtgc atgaataagt ataacactta tttcagtttt attaccatta
    20041 gcttcatttt gtatcaattc tgtttggaat ttatgagctt tatgaattga ttctttcgac
    20101 ttttcaatac attctctagc tttatctata ttcccgctct ttgcttcttt tattgcttca
    20161 aatgctagcc ctttcgcatc acctgcaaat ccaattattc caaaagatat ctctatcatc
    20221 ttctcatcca tttattcgac ctcctaaata tctctatagc ttgatatgac taatccattc
    20281 ttttctaaaa attctttgac ttttttagat gtaagtatct tgtgctcttt cgttctatca
    20341 atagcataag atgtagaatt taaaatataa tcatctaaaa aagctgggtg agacataata
    20401 tccactacat catacttcat tatttcgtct atatttttta taaaatattc ttcacttaca
    20461 ttttccttat aaaaactatc tattaaagga actactttag aatactctaa attatattca
    20521 aatccacctc ttattggaag attatacttg gttacaatct tctctattac tggctttaaa
    20581 cttactaaag tatggatatg atggtgacta tctaaatgag atatatctat tccaagccct
    20641 tttaccctat ctatttgagc acaaagttct ctataaatct catcacaatc cattttttca
    20701 ataatttcgt ctgtaatcct cctaataaaa aaaccgtctt tatcaacaat agtttttaag
    20761 ttagataata atggtctacc acaacttaaa gtcatatgaa ctccacaatt taaagtttta
    20821 ttttctttaa gtaagcctac tccatgttct accccaggca tatttgccat catagaagta
    20881 gacctaacta taccattatt atgagctgaa attattccat agttcacagc ttcacaatat
    20941 ccaaaatcat ctgcatttat tataatttta gtcataccat aacctacttt ctatagacaa
    21001 ctatctatta aatctaggaa gataatcttt atgagcttcc aacatttcgt ctaatatagt
    21061 ttttgctaag tcatcagatg gaatcaatgg gtttatacaa agtgccaaaa gagcagattc
    21121 ataatcgcca tatacagctg ctttagctgc caatacttca aatgatttaa tttgacttac
    21181 taatccatgc acagaatctg gtaaatatcc tatacttatt ggttttggac cattttttgt
    21241 tattacacta cttacttcta ccgcttgttc atctttaaaa tctcttatag aacccttatt
    21301 taatgtatta actacttgga tatctttttt gtcgttgtat atagaactta ttaaattaca
    21361 agcagcatca ctatagtaag cccctcctct tttttcaagt tgaggtggtt ttacatctaa
    21421 tttttcatcc ttatataatt caaataattg ttcttccaat tctttaacta cttgacctct
    21481 agcttttccc tctttaaact ctctcaactc atcctcaagc atttctttag ttttatagta
    21541 gtatctatga tatggacaag gtattgcacc caaagatttt acaaattcag cattaaaatc
    21601 aattgccttt atattttgca tacttatatc tgctttgaca aatttatcaa ttgcttcttt
    21661 tgtaacatct tctccatcta aagatacatt tagaccataa accatatgat taagacctgc
    21721 aaaatccata cttattctgt cactttctac attaaatagt ttggcaacat cattttttaa
    21781 atgaattggc acgttgcata gacctatata tcttctaaaa ttagtatatt tgaatacagc
    21841 ctctgttatt attcctgttg gatttgtaaa attcaccatc catgcatttg gacataattc
    21901 ttccacatct tttattatat caaatataac tggtattgtg cgaagggctt taaataaacc
    21961 tccagcacca ttagtttcct gccccataac tccatgagat aaaggaatag tttcatcttt
    22021 tattctcgca tctaaaagac ctactctaag ttgtgttgtc acaaagtctg catctcttag
    22081 agcttctctt ctatctaaag taagatttat tttcatatct actccagctt tttttaccat
    22141 cctcttagct agatttccaa ctatctctaa tttttctttt ccagcttcta tatctacaag
    22201 ccataattcc tttacaggta actcttcata tctatttata aaaccctcta caagctcagg
    22261 agtataactt gaacctcctc caattgtaac tatttttagt ccctttttca tctcattata
    22321 ccatccttac ctataattta atatactcta caaaacccca tacatttatt taaaactcta
    22381 tacatttatt taccgaaata aaactaccta gttgttaatt ttcaatttat tagcttccat
    22441 tttttcagac gctatgacaa atggaatata tattaatatt gataatatta aattaaatag
    22501 tgctaaagct ccaccagtcc agtcacctgt agccattgct ccacctaata taggaggtgt
    22561 tacccaaggt attttaggta aaattactgg tgctactaat ccaaaatcta ttgctaagta
    22621 tgatacagca ctacatatta ctggtgcaag tacaaatgga atcataaata taggatttaa
    22681 aactattgga agtccaaata tcaatggttc tcctatgtta aatattgcag gtggaccacc
    22741 taatactatc atctctttac gtcttcttcc agctattaat aaagctacta ttaatcccaa
    22801 taccattcca gaaccaccta aatatacaaa tgcatcaaag aatgcacctg caagtacatg
    22861 gtatccttcc gttgctcctg cttgagccaa ggcagcattt tctccaccta atttcataag
    22921 tatagtttct gtaaaaggaa gtgctatatt tgcaccatga agtccaacag tccaaagtat
    22981 atgaattata aatgcaataa gcattgtaga acctaaacta tcagctacat ttgatagtgg
    23041 tgctcctaaa taagtattta aaatatctgt taagaaactt ccattagaaa gcatttttat
    23101 aaatattcct attacagtaa atatcattat agttaacatt cctggtatta actttgcaaa
    23161 tgatcttgaa actgctggtg gaactccatc tggcattttt atagttaatt tctttacctt
    23221 agataatctt acaaatatct cagttgataa taatcctatt aatatagaag agaataaagc
    23281 tgctgtacct aagtaagttt gttgtatcat tccccatcct tcaactactg tagttcctcc
    23341 ctcaggaact attttaccaa tttgtggagc cattataaaa aagatagata aagctataag
    23401 cccagattgt aacccatcac tctcataaga ttttgctaga aaataagcaa cacctattac
    23461 taagaatact gccattgtac ctattgctcc ccaccaaagg tcgcctccaa atgtggtcca
    23521 gttttctcca aatatacttg ccatcaagtt tttgtatgct tccaatggta agttatttat
    23581 caatgttccc aatgcaccta ccatagttat aggtatcatt actataaatg catctcttac
    23641 tgctactaag tgcctttgag ctcctatttt agcagctaca ggcactatat atttatccat
    23701 aaatgacatg aatttttcca ttttacatcc tccagtacaa ctttattttg aactatttaa
    23761 ctctacagct ctatctaaca cagcttttcc attacatctt ccataatcca tcatatttat
    23821 aacttctact ggaatattat atggttttgc catttcagtt gcttttccta atacatatct
    23881 aacttgaggc cctaaaagta agacatcaca attttctatt tcatttttta agttcacttc
    23941 tgatatagcc catatttttg cttctatacc gttttccttt gctgcatcct ccatttttgt
    24001 aactaataaa ctagttgaca ttcctgctga acaagctaac attattttta tcattttttc
    24061 ccctccaaat tagattcatt aaatattttt taagtcataa gttttttaaa ctatacattt
    24121 aaaatatgct tttctattaa ttttttctta atgtatatat cttattttta aatgtatata
    24181 catttaattc ttgatattat tatataccaa atgtatatac atttcaagaa aactttttca
    24241 ttttatttaa attttattcg acaacagcat tcaaagttaa tgtatatcca tagtgatatg
    24301 tgtgtgagta ctcaaaaatc tcactatttt gtaacttaca aatttggctt atatataaaa
    24361 gtggctctgg ttcttctaat tttagattat ccatacattc ttcattcgat attacagctt
    24421 gtacttctct ctctgacatg gctatcttgt atccacacac ttcttcaaca aaattataaa
    24481 gagaattttc acaatgagtc ttatttaaat ttggaaataa cttaactggc atataagtaa
    24541 tcatatatga aactcttgca tcattagcta atcttactct ttttatttcc caaacatttt
    24601 cttctaaact tatattcaac ttacttgata aattttcatc tgcctgaact atttcaagag
    24661 ataccaatat attagatact ttatagcctt tactttccat ctctttagta aatccactta
    24721 cagacgatat attatcatat atattatttg caataactat actacctaca cctctttgtc
    24781 tagttatata gccttcttta actagattat taagtgcaga ccttacagtc attctagtta
    24841 cattaaattc ttcactcagc tgtttttcag aagggattaa atccccttct ttcaaactgt
    24901 ctgaatttat taactctcta acatgatttt ctataacttt ataaattggc tctttcattt
    24961 aattcactcc ttattaaatc ttttatataa taagataatt ttatatacaa acaaagaaaa
    25021 tgtctataca tttaagataa tttaaatttt attcagaaat cttgtacaac tttccgagtt
    25081 ttaataaaga tataaataga cttatcatta atataccttt taatatactt gataaggtta
    25141 tactccacca tacaccattt agacccaaca tttctggtct agatataaag taagccaatg
    25201 gtactcttgc accagtcagt atagtaatta taatagaagg tatataggtt ctccctaatc
    25261 ctttgaaagc tcctgttgtt attatttcta aacacataaa aaattgagaa tagcctaata
    25321 ttttaagata aactgctcct ttttctattg cctcactttc atctataaaa atagaaaata
    25381 tactctctcc tgcaaacact aatactaaag tagttattat accaagtaca gaagatacag
    25441 ttatagctat tttgcaacct ttatttattc tagaatactt cttggcacca taattttgtc
    25501 caacaaaact gcctaatgca actgatagcc cttcagccgt catccaggaa attgcttcta
    25561 tttgtgagcc tactttttgt actgcaacag ccacaggccc ccatgatgct actattactc
    25621 ctaatagcat tgagaatact gtaaacatac cactttgtat tgctataggt aatcccaatt
    25681 tatacagaac tttataatat ttagtctcta tttctttaaa tagtcttaat ttaaaatatt
    25741 cttctttact tttaactatt atatatataa aacatgtagt aactactatc tgagcaaata
    25801 cagttgcaat agcagctcct gcaacaccaa gttttggagc aaatccccat ccaaaaatta
    25861 atatagggtc aagtataata ttagttatta atcctactgt attaatacga aaaggagttt
    25921 tactatttcc aagaccattg aaaattgcag taaatactgg attaataaaa taaaatacca
    25981 tacctagagc tactattatc aaatactgtc tagacatggt tataacttct aagtctccta
    26041 atctaaaaaa acctatcaat tgtttattaa gtactattaa agaaattgta tatattattg
    26101 ctagtataat atttatttct attgctgact tcatatatga tttagtagtg cttatattat
    26161 ttgcaccaat actttgtgct acttttatct ctccacctac ttttgaaatc ataataaatg
    26221 ccattgcaag ccatggaaaa aaaccagctg ttccaactgc agctactgct ttacttccgg
    26281 ctttaccaac ccatatcata tctatcatgt tataccccat ctgtataaat gaagtcccca
    26341 ttattggaag tgatagctta agtaattttt ctgtaattct tccttctgtt aaattaagtc
    26401 tgtctttcat tttccacctc ttttatatta aatttatttg cttatatagc tagattaatc
    26461 attttatttg attgagttca ctatccttca atttatatta tcatatctca ggattgcatc
    26521 tcaatcatac ttatgtttaa gatacagtat tttacctatc tatgataaaa ttaatactta
    26581 tgaatataaa tctagccatt tcatcaatac atattctaac ctttaactta acttcaatta
    26641 agtatacata tcgtaaccaa ttagaatatt ttatacggta tcttccatta ataaacaacc
    26701 aatatcaatg atttatatat aattttattt atacataaaa tagtatacta taataataaa
    26761 aaaactatat ttgaatgctt taagcatgtt tactaattgt aatttccaca caaatttgat
    26821 aatatatgaa tatattatgg tgaaatttat agatatatat taattatggg gagattgtgg
    26881 gaggttcttt tcacaagaca tttaagctat gaaaataaat aaaaaattca atagagttga
    26941 tatgcaggtt tcaatactta ctgcaattat tgtaataata tcttcctcat gtgtatattt
    27001 ttttaattat acactaactt ataagagcat gatatactct ttaagtgaga ggtcaaaaca
    27061 tatctatgaa tatgtagaaa gaaatattga taaaaatacc tttactgaaa ttaattctaa
    27121 agaagaccaa tcaaaaaagt cttataaaag ttctaaaatc cttttagaat ctgtaaaaaa
    27181 tactactgga gtcatgtatc tatatactgc taaaaaaaca aaagatggat cactagtata
    27241 tgttgttgat ggtttaaatt catctaggag tgattttaga aatgctggtg atttgataga
    27301 aaaagagatt tggggagacc ttaacaaagc attagataat catatattgt tacctaaaaa
    27361 gattaaatca acatcttggg gatacatatt tatatcgtat tttcctattc acaatgatgt
    27421 tggtaaggtt gttggagtaa taggaattga atttgaagca aaacatcaat acaatacatt
    27481 taaatttata agtatagtta caccaataat agcattatta acttgtctaa tatcagcatt
    27541 gattgcagtg attctattta aaagaatatc taatcctaca tatactgatt ttgctaatac
    27601 agattattta acaggcctaa aaaatagaaa tgcttttgaa atagatatga ataacttaaa
    27661 caattcaaat ctaaagaatc taatatctat catatcaata gatttagacg gtttaaaaaa
    27721 gattaatgat aaatttggcc atcagacagg agacttatat attaagcaag caagtaaaat
    27781 tatacaatat gtaataaata aaaaatatat agtttatcgt attggaggtg atgaatacat
    27841 aattattcta aaaaatttat ctcacaaaga aataaataat attataaata atattaattt
    27901 aaaaatagtt gaagaaaact caactaatga attaaaatta tcaatgtcta ttggatatgc
    27961 tatttttgat gaaaaagtag atgtttcttt atttgataca tatcgtcgtg cagattctca
    28021 aatgtatgat aacaaaagga aaattaataa acaatatcaa taataaattt aaaaagtcta
    28081 ttctttaaat aataaatcac aagaatagac ttttttaaat ttgtgttttt ataagataaa
    28141 ttatttaatt aaagtttgtt cttttttcta aacgattctt tataaattca tatattactt
    28201 tcactaatat atttgtgact aatattaaca cagctagtga tgctgcctgt gcagtactcc
    28261 ccttatcatc tagttgtaca attgatattg cagctacttt tgaacttgca gtatatataa
    28321 aaactaatgc tgatattgtc accatggagt ttaggaaaaa gtacataaag ttctctaaaa
    28381 ctgctggcaa acaaagtggt atagttacgt tcttaagaat tgatgcgctg tttattccca
    28441 ttgatttagc taccatatcc aattctttat ctactttctt aatagcagta gtagctgtta
    28501 taaatgctac tgaaaagaaa tgaactatat tacatataac cattataagt aatgttccat
    28561 ataatccatt aaacaaattc ttaatgtata ttgtatccat aaaattaaac tctaatttat
    28621 taaaaaataa tacataagct atacctaaaa ctaatcctgg caatgccata ggcaatgtac
    28681 ttaaaaaata cccaaatgat tttaaatatg gagctttttc tgttttttca ttcaaatatg
    28741 caaataagaa aacaaagctc gtccctagaa tagctgtcaa tagtgacatt tttattgaat
    28801 ttataaatgg tttaatccca tcaatactgt ttttcataga aaaatgttcc agtgtcagtc
    28861 ttaaatcata tggccaaact gatattaatg aagataagat tacagttaac attacagcta
    28921 ttataccaaa tgcaataatt cctacaaccc ctcctaaaat actatctctt aacttattag
    28981 ttttaacctt atatggaatt gactttgaag ttatatatga attttgattt ttttcaattt
    29041 ttttagttac tataaacgat attattgcag gtattaaaag taatattgat acagttgctc
    29101 ccattggcat attctgttgc cctataattt gcttatatac atctattgat aatacattaa
    29161 actctcctcc tatcaccttt ggagctccaa aatctgtaaa acataatgta aacgatacga
    29221 atgcagaact tattaaagag tattttatac ttggtattgt tatgcttata aattctctta
    29281 tcttgcttat ccccatagta ttactagctt catatagtct ataatcggtt gaacttagag
    29341 atatataaag tatttgaaat gattgaggaa atacatatat aatttgtgcc aatattattc
    29401 ccactgctcc atatattgga acatgcactc caagtaaatt agttaccaaa ccttgatttc
    29461 caaataaata tactagacct attgcatgca tcattgttgg agcaaacata ggaaacatag
    29521 ctaatgattt aaaaaaacct ttaaatttaa tctttgttct agttaatgca tatgcatata
    29581 ctaatgcaag tgttagtgat attacagttg atataactga tacaaatact gtatttttta
    29641 gagaatttaa tagagattca gaagccaagt attcttgaaa gttttgtaat cctacaaaga
    29701 ctccatcttt atctaaaaaa gcctgtacaa atagatatat tataggtgct aataaaaata
    29761 ttactaaaga cataactaat actgtaagca tacatttttt tatatctaaa ttcatttttg
    29821 atttaagact tatactttta gaacttatag cattattatt catacactta tgctccttta
    29881 acttttggat actttaaata gtatttttcg tctatactta tttttacaag gtcaccttct
    29941 actaaattta aattaaccca ctcttttata gaagcatcac ttattatgaa attttcattt
    30001 aatttatctt ttatttcaac tctcaataaa ttccctctaa actctatatt tgatattatt
    30061 ccttggtagt tttcttttgt cgatttctct atttgaacat attctggtct tacagttaat
    30121 atagaatctc cattatcaaa acagtttgta tctcctataa attgagctgt aaataggttt
    30181 tgaggatttt ggtatatttc ttctggagtc cctatttgca ttatatctcc tccatttaga
    30241 atagctattt tatctgccat agttatagct tcttcttggt catgtgttac cattattgtc
    30301 gttatattaa gttctttttg taatctttta atgtccattc taagcttgtg tctaaccttt
    30361 gcatctaatg cggacattgg ttcatctaaa agtaaaaact ttggttcaag agctaatgct
    30421 cttgctattg caatcctctg ttgttgccca cctgataatg ctttaggata tttatgagct
    30481 tcattagtca atccaactgt ttcaagtact tccttaactt tattatctat ttttcctttt
    30541 gaaacttttc tctcttttag tggaaaagct atattgttat aagcagtcat attcggaaat
    30601 aatgcatatg attgaaatac tataccaaaa cctctctttg atggctctaa atttgtaata
    30661 tctttatcct ggagaattat agttccgcta tttacatctt caagacctgc tattattcta
    30721 agtaatgtag ttttaccaca tccactaggt ccgagtaaac atagaaattc tccttcttca
    30781 atatccaaac taatattatt taaaaccctt ttttggtcat aacttttaaa cacattattt
    30841 atttttaagt aactcatatc ttatctctcc ttatatagtt atttttttat tttaaatttt
    30901 atatcctact ttttcttcta aaattctttc atctaaatta ttaatttaaa ctttacttac
    30961 tttcagattt tgaaccgtat tttgattccc aagttttaag tactgtactt ctgtttttgg
    31021 cagcagagtt taaatcaact tcttttataa gctgttcaaa tggttgttta gaataacctt
    31081 ttggaactgg attatctgtc tttatggaag taactgctac ttcatgtgca tatctatcca
    31141 tagctgaatc acttattgcc caatctaaaa atagctttgc ttcttttttt atattatctt
    31201 ttttaactaa ggcatttgct tctaagtccc agcctgaacc ttcctttgga aatacaacct
    31261 ctataggttc tccactagat aattgttgtg tagctctata tccaagtgta atacctattg
    31321 gatactctcc agagccagct aacttcgcag gttttgaacc cgagtgtgta tatgtggcta
    31381 tatttttatg taatttatcc atgtaattgt aagcatcttt ttcacccatc atttgaataa
    31441 gccctgatac tgtaagatac cccgttccag atgatgctgg atttggcatt gttataagtc
    31501 ctttatattc tggttttatt aaatcttcat aactttttgg aatatctata cctagtttct
    31561 taagttcctt ataattaact actattgctg tctctgggac actcattcct acccaagata
    31621 catcatcttc agtatcttta aattttttat caactttatc ataattttta ggcttatatg
    31681 cttcaagtgc accatctttt tttagctcta tcatgttcat tgctgataat ccccatataa
    31741 tatctgcttg tggattatct ttttctgcta atagttttga agctattaca ccatgagaat
    31801 ccataactat ctctacatct atattgggat actcttctct aaaaccttcc atataataag
    31861 ctatctgctc ttcctctagt gctgcatata ttgtaagttt attatttcct tttccttgag
    31921 tagtttgtgg tgttgttgat atgcaacttg tcataaatgt aatcactaca atgcacagac
    31981 aagtaaatat aaattctttc atatcttccc cctttgtgtt ttttcctttt tttatttgtt
    32041 ttttattgtt tttgcttatg tttatataat acacctaatt tgttaatata aagttaagtg
    32101 taaattaaca gttcgttaat ttttagaaat ttttgttaaa tatattattt ttagttaaat
    32161 acagtctgtt tttaaacaat atattaattt ttatagtatc tacattttaa tttaaacata
    32221 aaaaataagt ctaacctcta taaagtagaa tgttagactt atttttataa gtatttaatt
    32281 atacaaccaa atcatttttt ttcttttctt caagtgcttt tttaagcacc atatccttaa
    32341 ccttcataag tctagtagta cttgctctct cattttcttc aagcttcata gctatatact
    32401 taatagtctc aatgtaattt ggtatcatta cattttcaag agcattaaca cgtctacgtg
    32461 tcttttctat ctcttgtgca agaagctgtg cacttttttc actttctgca agtcttaaaa
    32521 gtggttgcat agcatctgag aaagcttcca tagcagaatc taattcacct gatgtaaaag
    32581 caagaccata aggataaata tcactttgat tgttttcagt tttaaagtca aacacaggaa
    32641 catcaacact cattatattt ctcgtagaaa catctactga aactgattgc tttggcatca
    32701 taagtgctga acccagatac tcctgactca taactgctct tgcgattata aagtttttat
    32761 aagcagtatc aagagcattt tctgcttctt cacgcaatcg cttattttct cttacaatct
    32821 caagaaattg tttcataagt tcatcaagtt tatctttaag aagcttatga cctcttgtag
    32881 cagttttaag gagtttctta agtctagtca tttccatacg tgttggattt atatttaatc
    32941 tagccacaga tatcactcct taggcatata tttttcaaaa tattcatcac ggatacgttt
    33001 aagttcactt tttggtaaca tactaagaag cttccatccg atttctaatg tctgctcaat
    33061 agttctatca gttctaaatc cttgtgaaac atattctttc tcaaattcat ctgcaaactt
    33121 agcatacatt aaatcaatct cagaaagtgc agattcacca agtatagcca taagttcttt
    33181 tgcatcctta cctcttgaat aagctgcaaa aagctggttc attgtatctg catggtcttc
    33241 acgagtctta cctttaccaa tacctttatc ttttagacga gaaagtgatg gtaaaacatc
    33301 tataggaggc tgtatatctt ttttataaag ttcacggcta agtataattt gcccttctgt
    33361 aatgtatcct gtaaggtcag gaattggatg tgtcttatca tcctcaggca ttgtaagtat
    33421 tggaatcata gttatagacc cttcatgccc tttcattttt cctgctcttt catacatagt
    33481 agcaaggtca gtataaagat atcctggata tccacgacgt cctggaactt cttttttagc
    33541 tgctgaaact tcacgcaacg cctctgcata gtttgtaata tccgttatta taacaagtac
    33601 atgcatatct tgctcgaaag caaggtattc tgctgctgta agtgccattc taggtgtaga
    33661 tacacgctct acagctgggt catttgcaag gtttacaaac actacagaac ggtcgattgc
    33721 accagttgct ttaaaatcgc ttataaaaaa ttcagcatct tcaaaagtta taccaactgc
    33781 agcaaataca acggcgaact tagaatcagt tccacgtact tttgcctgtc ttgcaatctg
    33841 aactgcaaga ttggcatgtg gcaaaccaga ccctgagaat ataggaagtt tttgccctct
    33901 aaccaatgta ttaagtccat ctatagcaga gactccagtt tgtataaact cggccggata
    33961 atcacgtgct gctggattta ttggagctcc atttatatca agtcttttat ctggtataat
    34021 ttctggacct ccatcaatag gtctacccat tccactaaaa acacgaccta atatatctgg
    34081 tgaaactcca aagtcaagac tctttcctaa aaaacgaact ttactttcag caaggtttat
    34141 acctgttgag ctttcgaaaa gctgaacaag tgcagtatct ccatttacct ctagaacttt
    34201 gcaacggcga atttctccat ttgaaagttc aatttctcca agttcatcaa attttacacc
    34261 ttctacgccg tttattaaca taagaggacc ggctacctct tgtattgact tatattcctt
    34321 tatcatagtt cttcagcccc cctcttttta ataaggtctg caagctcgtt gtctatttca
    34381 tcttcaattg aatcgtaagc tgcatctata tcgccttctt ctacatactt agcacgacca
    34441 atcttttcaa gaactgaaag cttgataata tcgtttatag taacattttg cttgatagca
    34501 tcttgtgctg acttatagaa ttttaatata agtcccatca ttctatattg tttatgaagt
    34561 gatgtataag tatcaacttc atggaaagag ttttgatgaa ggaagtcttc tcttatagaa
    34621 cgagtaacct ctagtatcaa tctatctcca tctgaaagtg aatcaacccc aaccaattga
    34681 actatttctt gaagttcaga ttcaacttgt aaaagtttca tagcttgagc tctttgagct
    34741 gagaaattat catttacatt tttatctgcc catacatcaa ctttttcctg atagagtgaa
    34801 taactagtca accagttaat tgctgggaaa tgacgtttat atgctaaaga tgcatcaaga
    34861 ccccagaata cttttactat acgaagagtt gcttggctga caggttctga tgtatcacca
    34921 ccaggaggag aaactgcacc aattgctgta agtgttcctt ctctttcatc catacctaga
    34981 caatttacct ttcctgctct ctcatagaat tgagcaagac gtgaacctaa gtatgcagga
    35041 taaccttctt caccaggcat ctcctctaaa cgaccactca tctctctaag agcctcagcc
    35101 catcttgatg tagagtctgc cataagtgca acactatatc ccatatctct aaaatattca
    35161 gctattgtaa taccagtgta tatagaagct tcacgtgcag caaccggcat atctgatgta
    35221 tttgctataa gcacagttct ttgcataagt gactcgcctg ttcttgggtc ttttaattca
    35281 ggaaattcaa gaagaacatc tgtcatttca tttccacgct cgccacagcc tatatataca
    35341 actatatctg catctgccca tttagcaagc tgatgctgtg taacagtttt tccacttcca
    35401 aaaggtcccg gtatagctgc taccccacct tttgtgattg ggaaaaatgt atctataact
    35461 ctttgacctg taacaagaag tgaatctggt gttttctttt ctctatatgg tctttctttt
    35521 ctaactggcc atttttgcat catagttaca ggtatatcat tacctttttc atctgtaata
    35581 acacaaactg tatcttcaac agtaaattca ccagaatata tttcttttat agtaccctta
    35641 actccataag gaaccataat tttacattct actactgctg tttcttgaac gccaccaatt
    35701 atatctcctg ctacaacctt atcaccaaca gattttttag gtttaaattc ccacttagtt
    35761 gttctatcaa gtgaagatac ttcaacacct ttagttatat tagtacctga aatatcatac
    35821 attttttcaa gtggacgttg aataccatca taaatagttg aaataagacc tggtccaagt
    35881 tcaacactca taggcattcc aagagaaact acttcttccc ctggtcctag accggaagtt
    35941 tcctcataaa cctgtataga agctttatct ccatgcattt ctataatctc gcctataaga
    36001 tgtttatctg aaactcttac gacgtcaaac atattcgcat ctctcatgcc ctccgctaca
    36061 acaagcggtc ctgacacctt tacaattgtg cctgttttca tattctctca cctgcaatca
    36121 tatactttat caaaatttaa atattccctc ctatatttta ttagcacata tatttagcac
    36181 caataaaatt tgagattgtc ttttattcaa ctttcatcta ttctttaaat aatatatctg
    36241 cacctatagc acgtttacta gattctctta cttcattcat acctaatccc atacttcctc
    36301 caatacctgg aataactata atggcaggaa gttggtaatc tttgtatttt gctattgatt
    36361 cttttgcaag caatgatgcc tgttctgtaa tataaattat cgcatattca tcttcaacca
    36421 acttatgtat acttttttta atttcgtcac tatcataggc tgggaagata tctattccaa
    36481 gagctaaaaa tcccatgatg ctatctttat ctccaactac tcctacctta tacataagca
    36541 tcccttagcc tttcttttat agtatctgcg tctatattat taagcttact agtcaaaata
    36601 attctaattg tttttatctc tgattcttta gcatataaat atgcaattag tggttctaat
    36661 gtaagaggct taaattttgc tcctctaaca tattccataa gatagtcatc acaaagtttt
    36721 tcaaagacag taaaaccact atccatgcca tttttacaga catcagagta agaagtagat
    36781 ttaaaacatg atgctggagt atctgaggaa aaagcttcta agaatttttc cttatcaagt
    36841 gagccacctg aaacaaacac attcataaac atatcaaaat ctttcttcat atttttgact
    36901 cttataaaac tctttagatt tgttaaatca caaactttca taacataatc tattacaaat
    36961 ttgttttggc tttctttagc agtttctttc atactggtaa aggttgcttt atctagaaca
    37021 atatcaacca tctggccatt ttgtgtctta ttataaacat caaatgcctc taatatagct
    37081 gatgacatta tcttaggtaa atcactaaaa ctacgatttg ctaaagattc tctaagctta
    37141 ataagaggaa ttgtacctcc atcaattaaa tatttctcac catctacacc agaaatttct
    37201 tctttaatca gcacttttaa attgtgatag tcatttttat aaagaaatac atcaacaaat
    37261 ttttcttcag gagctagact tttaagaatt tcatatgtct ttgataattc ttgagtcaaa
    37321 atattctcaa aattatgtat attattattt gatgtatcag cataacctgc ctcagctata
    37381 attcttaaag agtcttcagg gcttttggct tctgccattt gaataaacat ttgtctgtta
    37441 agaagctgtt tttcaagcac tcttattcgt gcaacggcat agggataagt tttttcttct
    37501 gccatttttt aacccctcct taattaaaca aaatttctgc tgcaatttgt tcaatatatt
    37561 catgctctac tgcaattatg gcttcaaatg aataattgta ctcaatgtca ccttttttaa
    37621 ctatgaaacc tccagtaata tctctagttt catcagacac tttaatgcct ttttttgata
    37681 gcacatcttt taatgttttt ttatcttttt tagataatac tatctctgaa tcatctgtac
    37741 aatttgattt ttcaatcatg tttagtataa tctcttcata ttcattatct ttaagattta
    37801 aaagtttgtt tttagcttct aaaattacct cttctaaaat ctcttgtttt tttgataata
    37861 tttgcttttt agcctccata taagctcctg atatttcctt agaagccgct ttttctgctt
    37921 ctgcctctgc aagctttgta taggaagcca tttccttttc agctttttca ttagctgaat
    37981 ttacttttaa atctgcttca cttttagctt tatccaatat ttcttgtgct tcttgtttgg
    38041 catcagctat aattctgtca atcatttttt gttcattacc cataggagtg cttcctcctt
    38101 ttcatttttt ttacttatga attataattt ataattaacc aattgcaatt ccattgtaag
    38161 ctaagaaaga aacaagtagt cctaaaagag catatgtttc aactattgct gcaaatgtta
    38221 tagctttacc aagttcttca ggtctttttg caactattcc aactcctgct gctgctgctt
    38281 taccttgtgc tatacctgat actaaaccaa ctaaaccaat tggaagtcct gccataagaa
    38341 gttgtaaacc ttgagctgat gttggtattg catctccacc aataattcct gcttttgata
    38401 aaataaggaa agctatgata aatccataca gaccttgtgt accaggaaga agttgtaaaa
    38461 ctagaagttg accgaattta ctagggtctt ctgttacaac acctgctgcg gcttgacctg
    38521 ctattgaagt acctttagca gaacccatac ctgcaaataa tgctgctata gcagctcctg
    38581 atattgccaa aaattgacca tttcctaacg cgtttaaaaa ttccatattt acatctcctt
    38641 tttattaatg cttgtatatt ttgttttata cttaaacgga acaaaaggaa ctcctccgcc
    38701 ttcataaaac ttattgaaaa attctacata ctgtaaccta cttgtatgaa catatgcacc
    38761 tagtgcattt ataagaaggt ttattgtgtg tcctacaaca ccaattacaa ctgcaagtat
    38821 cggaccagct atagctccta aaaggttgat aacttgtgct ataactcctg ttgttaaaca
    38881 aagcgccata attcttgtat aagataatat atcagcaaaa taacttgtta taccatagag
    38941 acttgaaaat cctttaaaaa gcttaacagg aataccttta aagcttcttc cttgagtaag
    39001 tacaagacct atagcaccta taattgctaa atattttcct acttctgaaa atacacctat
    39061 atcccctgca acaaaaggta ttataagtag acatacacct gttatacaca aataccaaaa
    39121 acctatgtca aagattgcat ctattacctt tccacttctt attaatgtat atgcttttat
    39181 tcccaaacct acatatatat ggataatacc gaacagtaac gacattccca taagtgtcat
    39241 aacatcctct aaaggattaa ttaatgcttt tataggaatt aaatccccca aaataccccc
    39301 gaatataaga ccccatacag tagttgaaac tccacagata ccaataagtt taattaaatt
    39361 tccttcacct ttttgtatct ttaatttata tacaacaaat ccacagacta ctgcaattat
    39421 taacccatat gcggcgtcac ttagcatcat tccaaagaat acaacaaaga agaaagttaa
    39481 aattgtattt gggtcaatat cttttgtact tggatagctg tacatgttag ttacactttc
    39541 aaaaggtgtt actaatttat tgttttcaag cagaattggg aagccttctt ctttacttcc
    39601 ttcttcagtc tgaacacaac aattaaactt ttctgtaatt tctttaatta attcatctgc
    39661 ccttttgctt ggtaaccaac cattaagaca gaatgttgtt tttgttttga caagtctctc
    39721 tataatcttt ttttggtctc tttctatagt gtaaaaatca tataggtttt caagcatgtg
    39781 tatactatct gcatgtcttt taatttcctc tgttttgtcc ttagaaacgt tttcctgtct
    39841 aattatcatt tcattgtatt tcttgatagc ttgtaacggt gtaccttctt catttggtaa
    39901 tgaaaccact gaaaaattaa attcttttaa aacttctaaa acatcatcaa aagtttcttt
    39961 taaagtaatt aaataaacat agttcattaa cttgtcagaa ttgacaatcc caataacact
    40021 ttctggtatt tcatcttcta attttactct taaagcatca atgtttgcgc ttatgggcac
    40081 agttccaagt atagtttttg tatacttagt ttccatattt tcaagaggaa tatcaaagtt
    40141 cacccatggt tctaacatac ttttattgtt gttaagtaag tttatgttag accttattgt
    40201 acctatctcc ttgtgcaaat tgtttaactc cagagcttca gcataaactt gcccttcttt
    40261 ttcactatca attttgttaa actctctttt tggtgcaagt agggattttt tttgtttcac
    40321 tagtgaagaa acaatattaa ttgcttggtt tacatgagac attttctgct ctagcattat
    40381 aacttcagat tcttcactat cttttacaag ataatttttt aattcctcct cctcagtaag
    40441 aaaggcacta tcatcaatct gaacaaaccc acggttcata agtagcttta aaatctggct
    40501 tttttgagct tctaagccaa caatagatat tttgttcata ttaactattg ccatattatc
    40561 tcactacctt tccaataacc gcttcaatag ccttactttt tctttcttca gcagtttttt
    40621 caattttcag acattcactt ttagcattag ctaagatttt atcaacctct gctctactgt
    40681 cggtgactgc cttctcaata agagtttctt ccataagctt tgcttgtttt tttacatcag
    40741 caaatatgct ttcacatttg tttacagcat cagctctgat agactctgca tcagtaacag
    40801 cttttacttt gatactgtct gctgtctgct ctgcctcaac aatgcttttt attatttcaa
    40861 aagccatcca aatcacctct cttacctttt tactatgtat ataaccaatt agattttttt
    40921 attctaatta gattactacc tcattgttaa agtataaaca gcatttttat ataataatta
    40981 atatttttca acgtttattt cttaataaaa catattttag aatagtactt gcctagattt
    41041 acatcctttt ttgacttact tttagactat gacatgagta tggtttttaa ataccattat
    41101 tttcaataat tataatttaa agcgatatag tttgtcaata gcgttatttt gttttaaaat
    41161 taacgttatg tcgacatttt aaatttttat tttatttttt ttataatttt aatttaattt
    41221 attacatata aaagtttttt atattctttt tcaacctcta accattgaag tttattgttt
    41281 tttttgaatt tttagaaata tctcgcacta ttttatctaa cttttataaa tcatcaagct
    41341 atatcgattt ttgtcacctt atttttaatc taattttttt atatctagtt agaaataaat
    41401 caatattttt aaagattgat aaaacttaca cctagttcta atagttttat tttaaggcaa
    41461 tgtattaatc ttaagtagat aatatcatta gttactctat atattcgaca acattataaa
    41521 aaaatcctcg taccaatcac tccaatcttc aataggatat gctgctcctc catttacact
    41581 tttcagtaga ttggatgtat attcttctcc catagaaaaa agttccccta ctgtcatatc
    41641 ataacctcta gatgtaacaa ccttacaaaa tgaatccata acatcacgtt gttctcttgt
    41701 ttttataagt tcttttctaa attcatcatc attcttagct tcttctaaaa ttttagttaa
    41761 tttttcattc attatgtatt tctcctttta agtagtcttt ctgtaaactt caataagctt
    41821 tatataaaaa ggaagccatc ctaaataaac taagatgact tcgcttaact gttagcaaca
    41881 catttactag tataaaataa ttggtgtacc tggttctata ttttcatata ttttctttgc
    41941 cttagaaaaa ggtgtattta cacatccatg tgaacctgaa gacttatata tacttccacc
    42001 aaaagaattt ctccaagtag catcatgaat gcctatgttc ccattaaacg gaagccaata
    42061 acttacaggt gaactgtaac cttgaccagt taatgaaaca tttctagcct tataattaag
    42121 aggatatata cctgatggtg tagcaaatcc tctagctaaa tcacctgtta caacgtctgt
    42181 tgatacaagc atttctccat ttttaaagaa ccacatatgt tggccagaaa gactaatctc
    42241 tacatatgta tttccaatat catctttatt acgagataca gctttttgta tgtaaatagg
    42301 ttctcttgta acatttttac cagcttttac aacatcaact aatgcttcag cttctttctt
    42361 tttatctatc aaccaaccat aaattccacc tgaaattgta atatctttac ctgtggcagt
    42421 tttaaatggt cttgagtcac caaaagtact gtatttttta cttaaacctc ttgtatattc
    42481 taccatagca tcattatcaa aagttatgtc ataattcttt tctgagtcta cttttaacca
    42541 tttattaatt tcagaaccat ctaaagtttc cttacggtca ctaaaatcat atgtaacgac
    42601 tacaccaaca tacttatcta atgtcttaat agcttgtttc aacttttctg aatcctttgt
    42661 gttgtttggt gcttcataaa ctttactttc atccagagaa atagaagtat tcccagaatt
    42721 aattgagttt tgtactaact tataaaaatc atcttttttc actttgtttc caagtacctc
    42781 tggtactata acataagcgt ttttagtgct atcatactta ggataagcac tcttaggttc
    42841 tacaacattt ttactatcaa aacaagataa agcattaaac cttttttcta gtaaatcttt
    42901 attatatgaa agagttatca tattattata ctctttagac ttaaatattc caccaatcca
    42961 tccaatagcg ctctgactat cttttatctt ttgaacttta tcatctggtg taaattttaa
    43021 atcaatatct tttgctttta tttgttcttt cttattgttt ctttcctcta agtttaaagt
    43081 ataggaatta gtactagatg ttaacaaatc atttacttgc tcaactgttt ttcctgaagc
    43141 ctttacgcca ttaatagtcg ttccaaagta aaaatgattt ctaaaaaaga ttgccattcc
    43201 tatataagct acaaacaaaa cacctactac tatagctatc ttaaatttta gatttttttg
    43261 tccactttct tgttttacct cttttccatc aatcataatt aaatccttcc ttacattgta
    43321 attacctatg taccttattg gtttttaaac aaaaccatgt atgccttatt ttatataccc
    43381 aaattactgt atcattattt atatttttaa atatatagtt atttctataa aaccatttta
    43441 tcttttagat aaaatgatta ttataaatcc cccctttcaa aatcccttaa acacaatctc
    43501 tgctatatta atatattacc atacttttaa taatcgtggg actatctatc tatttttgta
    43561 acactaaact actattttaa aaaagaaact aattttgtgt tagtttcttt atcttaactt
    43621 atttttatat tgaattttag cttaacataa cgttgttata tagattctat ttaattattt
    43681 tagtttctat atcttttcca tctgcatatt ctttaagtaa ttccttagca tatccaaaat
    43741 attcttcaat atcagattct tgactaaaag aaaatataca aattaatatt tcatttgcat
    43801 cttctgttat cattgctctt tcagaatcac tagttggact cccaaattta cccactgaat
    43861 cagataatat aggaagattt tctatattta ttaattcttt cccaataccc ttatactgtt
    43921 ctccttcatc tcctacagta aaacatactg gtgaatgtaa tttgcttaca ttatatgtac
    43981 ctactggata taatgatttt attgatatta aattgtttat ttctactata ttatttattg
    44041 tataaagacc cttcccttga ataattcttc taaccaatgc ttctgaagat gttctatatc
    44101 tacttggatt ttttccaagc tctttataag catttcttgc atctttaatt ttatctagtt
    44161 tcgtaatgtc ttcaactttt agactttgat ttatagtatc acaaaactca tttatttcat
    44221 taattaattt ttctgagctt ttatttactg ctacttttgc ttgaatgcaa cccagcttag
    44281 cattaggcca tctttcctta agtttgtcat caatacttat atctatcata cttaaacaac
    44341 tcctatacac taaattttta ttaaagtttt tatcatctat acattatagt actattcttt
    44401 tgttaataac atgtttatta gcaaaataaa tttattttta atataatttt tataaaatac
    44461 aattattttt tgctttatat tagacaatac attgataaaa taaaaaaaca ctaattattt
    44521 agcaatgatt tattaaaatc aagcattcat aatagtgctt tatataaaaa tcaaattttg
    44581 ttttaaagta aatctaaaaa attcttagct ggcattggtc gtgcaaaata aaatccttga
    44641 actaaatcac atccaaattg tttcattaac tcagcttgtt ctttactctc aacaccttct
    44701 ataattacaa ctaaatctag tgattttgct agagacacta ttgattttgc tatattgcga
    44761 cttctctcat catgttcaat gccaccaaga aaagacttat ctagcttcaa tatatcaatt
    44821 ggcatatctc ttaaacaact aatagaagaa tatcctgtac caaagtcatc catagaaata
    44881 gaaaatccat acgtcctcaa ctcatttaaa acattcttta aaatatttaa ctcattaata
    44941 gccacagttt ctgttatctc tatttcaatg aaatctgaac ttaaattata catttttatt
    45001 gttttattga taaattttac aatatctggt tgatacaact cgaccctaga taaatttact
    45061 gaaactggaa ctatcttttt accttgcttt ttccattcat ataaatcatt gcaaaccctt
    45121 tcaaatacaa atctcccaat ttttaaaatc aacttacttt tctctgctat tggaataaat
    45181 actgcgggac ttataaaacc atgttctgga tgattccatc ttattagtgc ttcactccct
    45241 atcatttctc cagttacaag tgaaaactta ggttgataat acacttcaaa ttgatttttt
    45301 accaatgcta tttttatatc atctaaaatc attgattcct cactaagctt attcctaaca
    45361 tcttcattat aaatagcata atttatattt ttacctttag caacagacct agccatattt
    45421 gctttattta cagccttttg aatatctact tcaccttctt caacaaaata aatacccaca
    45481 cacagagaaa tttcaaagac tatactcaaa ttactttcaa tattacttcg tatattatca
    45541 agttttctta taattttatt tcttccattt ttataatcac agaaaattgc aaaataatca
    45601 cttgaaagtc gtgcatatac ctcaccttct gttaattctt cttttaaagc ttttgctatt
    45661 gtaattaata aattgtcacc aaattcatat ccaaaagtat cattaatcat cttaaagtta
    45721 ttaatatcaa aatatactat aatataattt aaagatggtt tatctaaaag atacttacta
    45781 cattctctat aaaatttatt ttgatttcct atatatgtaa ctttatcttc ataagccaaa
    45841 cttaatattt ctttttcatt agattttttt atataaatta tataaaaaat aattgcaata
    45901 aatattaata caatagataa cactgcatat acagctctgt tgattatttg ttttgaatta
    45961 ctaaatacaa cagtttctgg aattgaaaaa attaaattcc agtcttttat ataattattt
    46021 tcactattcc cattatccat agtggcatat cctaaatatc ttctttcttc caacatattt
    46081 aatacagttg cacctgtttt attttcttgc aattcttttt ttaatctatt tacttcttga
    46141 atatcattat cctgttttaa caatttataa atattttttg ataggctatc cccttgtgga
    46201 tgaaggataa ttgtaccatt attttttaca acataagata tactattatt ttcataaaaa
    46261 gatatattaa taagtttcat cagagtattg atattgtaag tacaatataa aactccaact
    46321 actttatcat tatttaataa tggtactgaa atagcaatct tctttataga cttatttact
    46381 tcatcaacat atggacttga aacatacttc ttacctttaa ttgagttttt gaaaaattct
    46441 ctatctttca ctgaaaactt atcacctttg gtattttcag cattaccatt tatatcaact
    46501 attgccatag tgtaaaattt ttgattttcc atttcatcta aaagattttt cattttttcc
    46561 tcattaggta tatctggatt tgaagcataa tagttagaaa aaatatttaa aacatctaaa
    46621 tctttttcaa tttgtctttg tgctagttga gagccttgta ctgcaacttc ttttatatgt
    46681 atttttgcct ctgacataag aagatgtttt gtatcttcta tatagatata ccccattgtc
    46741 aaaaataatg atgttaaaaa aagaattgta attaatgtaa aacttttatt tctccctgtc
    46801 attcgactcc tcccctaatt agtcaatact tcatatagat aatatatcct ttttatacca
    46861 attcatcatt tatattgtat ataactattc ttaaatgtta cacataaaat caacataata
    46921 accataaaaa cattctttta ttattataat aacactattt aattgaattt taaattaaag
    46981 aataaaaact atccattttc tcaaacaaaa aaactcttat acaataaaac tttgttttat
    47041 gtataagagt ttttatttta tctattttat agcaaactta tcttccataa tatgaattta
    47101 aatacatatc ttttatttca ttcataagtg gatatcttgg atttgctcct gtacattggt
    47161 catcaaatgc ttgaattacc atttcatcta ttgaatccat aaacttagat tcttcaacgc
    47221 caaactcttt tatagattta ggaataccaa ctttaagttt taaatcttca atagctacca
    47281 ataaactttg gaatttctct tcatcagaat ttccttttaa tcctaaaaat gaagctattt
    47341 cagcatatct ctttaatgct tctggatatt tatattgaga gaatgctccc attttctttg
    47401 gtgcatctga agaattaaat ttcataactt ctgttatcaa aagagcattt gcaacaccat
    47461 gtggtacatg gtggaaagca cctaatttat gagccataga gtgacaaaca cccaaaaatg
    47521 catttgcaaa agccatacct gccattgtag aagcatttgc cattttttct ctagcttctg
    47581 ggtcttttgc accattatca tatgcacgtg gaagatattc aaatatactc ttaatagctt
    47641 gtaaagctaa tccatttgta taatcggttg caagcataga tacatatgct tctaatgcat
    47701 gagttaaagc atctatacca gaagcagatg ttaagctttt tggcatattc atcatcatat
    47761 cagaatcaac tatagccata tttggcatca gttcataatc tgctaatgga tattttactc
    47821 ctgtatcttg gtcagttata acagcaaatg gtgttacttc tgaaccagta cctgctgatg
    47881 ttggtatagc tacaaaatta gctttttcac ccattttagg gaatgtatat accctttttc
    47941 ttatatccat aaatctcata gctaggtctt ggaaatctac atctggatgc tcatacaatg
    48001 tccacattat ctttcctgca tccattgcac ttcctccacc tatagatatt attacatctg
    48061 gattaaattc tctcatagct ttagcgccta tcttagcaca ttccaaagtt gggtctggtt
    48121 ctacttcaaa gaatgtagtg tacttaatat tcatttcatc taataaatca gtaactgctt
    48181 tagtatatcc attattgtaa aggaatgtat ctgttacaat aaaagctctc ttcttattca
    48241 ttacatcttt aagttctttt aatgctactc ctaaacaacc ttttttgaaa taaacttttt
    48301 ctggtgctct aaaccaaagc atattttctc tcctctcagc tactgtttta atattaagta
    48361 aatgcttaac tcctacattt tcagatactg aatttccacc ccaagaccca catcctaaag
    48421 taagagaagg agctagttta aaattatata agtcccctat accaccttga gaagaaggag
    48481 tattaattaa aattctacaa gttttcatgg ctgaagtaaa cttatcaagt ttttctcttt
    48541 gatttacatc atcaatatat aatgaagatg tatgcccaaa tccaccatct tctactaatt
    48601 tttctgcttt tctaactgca tcatcaaaat cacttgcctt gtacattgca agtactggtg
    48661 aaagtttctc atgagcaaaa gcttcttcta tttcaactga ttctacttca cctattaaaa
    48721 ccttagtatc tactgggact tcaaagcctg caagttttgc tatagtacaa gctgtttgcc
    48781 caactatttt agaatttaat cctccattta ctaaaattat atttcttatt ttttctgttt
    48841 catctttatc taataaataa gcaccacgct ctttaaattc ttttttgact tcattgtata
    48901 tattttctaa tactataacc gattgttcag aagcacatat cataccattg tcaaaagtct
    48961 tagatacaag aattgagttt acagcagttt ttatatctgc actatcatca attattgctg
    49021 gagtattacc tgcaccaact ccaatagctg gttttccaga tgaatatgct gatttaacca
    49081 ttccaggacc tcctgtagca agggtcaaat ccacttcttt cataacagtt gtagttaatt
    49141 ctaaagatgg ctcatctatc catcctatta taccttcagg agctccagct aaaacagcag
    49201 cctcaagaac aacttttgca gcctctatag tagaattttt tgctcttgga tgtggagaga
    49261 aaattattcc atttctagtt tttaaagcta aaagtgcttt aaagatagca gtagaagttg
    49321 gattagttgt aggtacaact gctgcaataa cacctattgg ttcagcaatt ttagtcatac
    49381 caaatgcttc gtctttctct ataacaccac aagttttagt ttctttatat gcattatata
    49441 tatactctga ggcataatga ttctttataa ccttatcttc agcaattccc ataccagttt
    49501 ctttttgtgc cattatagct agaggaactc tttgcttatt agctgccaaa gatgctgcta
    49561 aaaaaatttt atctacctgc tcttgactat atgtagcaaa tattttttga gcttctctga
    49621 tttttgctag ttttttaact aagctttcaa cgttatttac agtatctaaa ccaactgaca
    49681 taacttcatt ttctttttta ttttctatta ttacttgttc tttatgagcc attttaacaa
    49741 cctcctgaga tagtttttta tttattatgt taatttatta acattatagc atttactttt
    49801 cctattttca agttattttt ttaacatt
//
LOCUS       DAELNY010000007        49559 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.007, whole genome shotgun sequence.
ACCESSION   DAELNY010000007 DAELNY010000000
VERSION     DAELNY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.007"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..780
                     /locus_tag="KPE55_000362"
     CDS             106..780
                     /locus_tag="KPE55_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491464.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNSYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            768..1928
                     /locus_tag="KPE55_000363"
     CDS             768..1928
                     /locus_tag="KPE55_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0491465.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSDHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2199..2672
                     /locus_tag="KPE55_000364"
     CDS             2199..2672
                     /locus_tag="KPE55_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG0491466.1"
                     /translation="MLSSTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            2958..3626
                     /locus_tag="KPE55_000365"
     CDS             2958..3626
                     /locus_tag="KPE55_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0491467.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3629..6097
                     /locus_tag="KPE55_000366"
     CDS             3629..6097
                     /locus_tag="KPE55_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0491468.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKITKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6337..7761)
                     /locus_tag="KPE55_000367"
     CDS             complement(6337..7761)
                     /locus_tag="KPE55_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0491469.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7780..8472)
                     /locus_tag="KPE55_000368"
     CDS             complement(7780..8472)
                     /locus_tag="KPE55_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491470.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8527..9303)
                     /locus_tag="KPE55_000369"
     CDS             complement(8527..9303)
                     /locus_tag="KPE55_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0491471.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9300..10070)
                     /locus_tag="KPE55_000370"
     CDS             complement(9300..10070)
                     /locus_tag="KPE55_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0491472.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10067..10771)
                     /locus_tag="KPE55_000371"
     CDS             complement(10067..10771)
                     /locus_tag="KPE55_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0491473.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11026..11538)
                     /locus_tag="KPE55_000372"
     CDS             complement(11026..11538)
                     /locus_tag="KPE55_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491474.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11864..12121
                     /locus_tag="KPE55_000373"
     CDS             11864..12121
                     /locus_tag="KPE55_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491475.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12264..12890
                     /locus_tag="KPE55_000374"
     CDS             12264..12890
                     /locus_tag="KPE55_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG0491476.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13137..13916)
                     /locus_tag="KPE55_000375"
     CDS             complement(13137..13916)
                     /locus_tag="KPE55_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0491477.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(13984..14445)
                     /locus_tag="KPE55_000376"
     CDS             complement(13984..14445)
                     /locus_tag="KPE55_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0491478.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14421..15098)
                     /locus_tag="KPE55_000377"
     CDS             complement(14421..15098)
                     /locus_tag="KPE55_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0491479.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15331..15717
                     /locus_tag="KPE55_000378"
     CDS             15331..15717
                     /locus_tag="KPE55_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0491480.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15720..17519
                     /locus_tag="KPE55_000379"
     CDS             15720..17519
                     /locus_tag="KPE55_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG0491481.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17874..19748)
                     /locus_tag="KPE55_000380"
     CDS             complement(17874..19748)
                     /locus_tag="KPE55_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0491482.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLLGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19753..21417)
                     /locus_tag="KPE55_000381"
     CDS             complement(19753..21417)
                     /locus_tag="KPE55_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG0491483.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(21431..22252)
                     /locus_tag="KPE55_000382"
     CDS             complement(21431..22252)
                     /locus_tag="KPE55_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0491484.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22351..23115)
                     /locus_tag="KPE55_000383"
     CDS             complement(22351..23115)
                     /locus_tag="KPE55_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0491485.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23112..23861)
                     /locus_tag="KPE55_000384"
     CDS             complement(23112..23861)
                     /locus_tag="KPE55_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0491486.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24365..24970)
                     /locus_tag="KPE55_000385"
     CDS             complement(24365..24970)
                     /locus_tag="KPE55_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0491487.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSISELDNLKYEWICMSHGTPCIK"
     gene            25149..25787
                     /locus_tag="KPE55_000386"
     CDS             25149..25787
                     /locus_tag="KPE55_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491488.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            25919..26581
                     /locus_tag="KPE55_000387"
     CDS             25919..26581
                     /locus_tag="KPE55_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491489.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            26581..27762
                     /locus_tag="KPE55_000388"
     CDS             26581..27762
                     /locus_tag="KPE55_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0491490.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(27943..30603)
                     /locus_tag="KPE55_000389"
     CDS             complement(27943..30603)
                     /locus_tag="KPE55_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0491491.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQDASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(30600..31283)
                     /locus_tag="KPE55_000390"
     CDS             complement(30600..31283)
                     /locus_tag="KPE55_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0491492.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEIASLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(31627..32580)
                     /gene="blaCDD"
                     /locus_tag="KPE55_000391"
     CDS             complement(31627..32580)
                     /gene="blaCDD"
                     /locus_tag="KPE55_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0491493.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(33121..35235)
                     /locus_tag="KPE55_000392"
     CDS             complement(33121..35235)
                     /locus_tag="KPE55_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG0491494.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(35319..36155)
                     /locus_tag="KPE55_000393"
     CDS             complement(35319..36155)
                     /locus_tag="KPE55_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491495.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLAFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(36750..37112)
                     /locus_tag="KPE55_000394"
     CDS             complement(36750..37112)
                     /locus_tag="KPE55_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0491496.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(37346..37810)
                     /locus_tag="KPE55_000395"
     CDS             complement(37346..37810)
                     /locus_tag="KPE55_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0491497.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(38239..39660)
                     /gene="nhaC"
                     /locus_tag="KPE55_000396"
     CDS             complement(38239..39660)
                     /gene="nhaC"
                     /locus_tag="KPE55_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0491498.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(39690..40754)
                     /locus_tag="KPE55_000397"
     CDS             complement(39690..40754)
                     /locus_tag="KPE55_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG0491499.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(40739..42148)
                     /locus_tag="KPE55_000398"
     CDS             complement(40739..42148)
                     /locus_tag="KPE55_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG0491500.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(42148..44352)
                     /locus_tag="KPE55_000399"
     CDS             complement(42148..44352)
                     /locus_tag="KPE55_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0491501.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(44346..44720)
                     /locus_tag="KPE55_000400"
     CDS             complement(44346..44720)
                     /locus_tag="KPE55_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG0491502.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(44798..46228)
                     /locus_tag="KPE55_000401"
     CDS             complement(44798..46228)
                     /locus_tag="KPE55_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG0491503.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(46221..46529)
                     /locus_tag="KPE55_000402"
     CDS             complement(46221..46529)
                     /locus_tag="KPE55_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG0491504.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(46650..47717)
                     /locus_tag="KPE55_000403"
     CDS             complement(46650..47717)
                     /locus_tag="KPE55_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG0491505.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(47973..49346)
                     /locus_tag="KPE55_000404"
     CDS             complement(47973..49346)
                     /locus_tag="KPE55_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0491506.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        16791 a         8358 c         5902 g        18508 t
ORIGIN      
        1 ttaatgatta agttttgaaa tataactaag aatttgataa tttattgaga tttttatact
       61 aaaatataca tactatacaa acttaattta gaagggagca ttcttatgaa caataaaata
      121 ctcgtcgtag atgatgagga aggtataata acactcttaa aagattactt tgaaatgaat
      181 agttatgagg tttacactgc tcaaagtgga aaagacgcat taaaaaaaat atccaaaaat
      241 ccagatatca ttctacttga tataaacatg cctgaaatag atggaattga agtttgtaat
      301 agaattagag attatgtatc atgccctatt ctatttctta cagctagaat tgaaaatact
      361 gacaagataa atggttttcg tgctggtgct gatgattata ttgtaaaacc ttttgattta
      421 gacgaacttg gagctagagt atctgcccat ttaagacgtg aacaaaggaa gtcaaatcaa
      481 actaaaatta aattttataa tgatttaaca atagattacg aaaaaaggtt aatttgtata
      541 tataataatc agatttcact ttcaaaaaag gaatttgata tagtagagct tctctctatg
      601 aatcctggac aagtatttga taaagaaaaa atatatgata gagtctgggg atacaatagc
      661 aatggaaata gtgatgttat aatggaacac attagaaaaa tacgaatcaa actatcaaaa
      721 catactcttg aaaattatat agaaacagtc tggggagttg gttataaatg gataaaataa
      781 ataatatgtc attaaaaaaa gctttctttg ttcttacttt atcttcacta attatagcat
      841 cagtgcttac tactttcgca tacattttac ttaataatct atataattct atacaagata
      901 agtatttaaa aacacctata aatacaggta ttataaccat agttcaaaac aatgtgaacc
      961 aatttagtga ttatgataaa acattactta atataatcaa catacttcag cttgtacttc
     1021 cacttatatt ttttatagga cttttattat tagctgatat aattttttac aaggtaaaat
     1081 taaaaacacc catagaaata ctcaataaag gtgcaatgga aatatccaac aacaatttag
     1141 acttttgctt agagtataat aataatgatg aattaggtaa tctatgtaat gcttttgaaa
     1201 aaatgaggtc tgaactaaat aaaaacaaca taaagatgtg gactatgatt aatgatagaa
     1261 aacaacttaa tgctgctttt tctcatgatt taagaaatcc cctaacagtt ctaaaaggtt
     1321 actcaaacta tttaacaaaa tacataccaa caggtaagct tagtgatgag aagattttat
     1381 caaccacaca acttatgtct gatcatatta acagaattga atactatgta gaaaatatga
     1441 gtaatgctca acgattagaa gatttagttg tttcaaaatc tatgtcgaat ataaacaagt
     1501 ttatagaaaa cttagatgaa aatatatcta tcattgctaa acaagaagga aaatctttta
     1561 cacttaaaag tcaaataaat aatataaatc tattttttga tgaaaatatc attcttagag
     1621 tcgtcgaaaa tataatatcg aatgctttta gatatgctag aaataatgta tccatactta
     1681 tatatcttga acaagaactc cttacctttg taattgaaga tgatggtata ggatttagta
     1741 aggaatctct aaaattagct ttaagacctt tctatagaga taagacttta aatgacagca
     1801 atgcacactt tggtatgggt ctttatatct caaaaatatt atgcgaaaaa catggtggct
     1861 ctatttctat tgaaaataat tctactaaca gtgctaaaat tactgctaaa ttctcaacaa
     1921 gaaattaaaa tgattttgta agctttcact gttcttatat aaaatttgtt agtttagaat
     1981 ttataaaatg tatttcaatt tacaaccaat atatatacta ttttgtagaa gttgtaagag
     2041 ttgcttcaat tataatattt tggcaaaagg catttgaaac aataatagaa atatatattt
     2101 aaatatgtag aatagtatga aattagaatt ttggaggtga ttatattaaa agttttgcta
     2161 ataatattac attctctcgt attgtgtttg cattgctaat gcttagttct acattctgtt
     2221 cactgccttt ctggatatgt tatttttggt gtggaattag tgatatattt gatggtctta
     2281 ttgcccgaaa gttgagacaa caaagtggaa taggtgcaaa gttagatagt attgctgatt
     2341 tcattttttc ggttacaatt ttggttactg tcggaaaaaa tattgctctt cccatgtatc
     2401 tatggtggtg tattgttgca atagcattgt tacggttgat tagttacagt attggttttt
     2461 ttaagtatcg cacattttca tcgcttcata ctttgatgaa taaaatcact ggaatttcta
     2521 tctttgcgtt tccattactc tatatttgct ttggtcttga tgttgcagga attatgatat
     2581 gtatcgtggc ttttctttct tcttttgaag aaatagcaat tatgataaaa tcaaaagaat
     2641 taaatcgtga tataaaaagt atatttatgt gaaaacttag aatttgtgac aaaaataatt
     2701 tatataataa tgaggaagta aaacataaaa aattatccac aagtttatgt gtaaaatagt
     2761 tttaaagcaa tagagttgta ttgtaaagcc tttagtgcat aaattacatt ttaaattaaa
     2821 aatgatactg aaactgctta tcagcactgc gaattatccg caaatagtga agacttttta
     2881 gatttaagct aaaagttggg aaaaaattga gttttaaaac ttacactctt cttatataga
     2941 aaaggagagt gttttttatg attataaaag caaaacaatt atcaaaaata tatggttcta
     3001 ataacaataa agttatcgca cttaataatg taaatttaga aataaactca ggtgaatttg
     3061 tttctgtaat tggaccttct ggaagtggaa aaagcacact tttacatatt ttaagtggat
     3121 tagataatcc tacctctgga caagtgttac ttgatgataa agatatatat aaacacactg
     3181 aaaaagaact atctgcactt agaagaaaat cctttggatt cgtctttcaa caatttaatc
     3241 ttttacctgt tctgacagca tctgaaaata tttcaatgcc tgtacttcta gataaaaaac
     3301 aacctgataa agaatattta aatgaaatct catcattact tggaattgca gatagactca
     3361 atcatcttcc ccatgaactt tcaggtggtc aacaacaaag agttgcaatt gctagagcac
     3421 ttattgcaaa accagatata atatttgctg atgagcctac aggaaacttg gacagtaaaa
     3481 gtggcagtga agttatgaat cttcttataa aaacttcaaa acagtttgga aagactttag
     3541 tggtaattac tcatgatgat agaattgcaa aacttgctga tagaaaaatt tcaattattg
     3601 atggtgttct tatggaggtg aaatagaaat gaagaattac ctttcacttt caataaaaga
     3661 attaaaaagc cagaaactta tgactacatt catcattata gcaatagtac tatcaactat
     3721 tatgacaact gtggttggac agtcaattgg tatcctacaa aatttaagaa ttgaacaagc
     3781 ccgttctttt aacggtgata gacatgtatc ctttcaccaa cttactaaaa atcaagttga
     3841 tgacataaag aaagatgata gagtttatca agctggtact tcaataactc ttggcagtag
     3901 taaaataaag gattctggta tctctgtact tgtaaaagag tatgataaaa caggactttc
     3961 caactacccc aaattaatga aattaaaatc tggaaatttg cctaaagata aaaacgaaat
     4021 agccttagat gaaaacacac taaagcttat gggtataaaa ccaagactag gagtgactat
     4081 acctatgaat ttagatattt cacttttaaa tgataccata ccaccatata attacacagc
     4141 taactttaaa ttgagtggca ttttagagga tgattataca ggatatgtaa gtggtatagt
     4201 aaatggtatt gtaggtaaag gtacatcaga aaatctatta cctaagagat atattttatc
     4261 ctctttggat tttaaaataa aacagcaaga aaagtttcaa gaaatagtaa atcaattagc
     4321 taaaaaaata aatctttctc acaattcaat tcaatataat tggatttact taaatgctct
     4381 taagatacag tttgaaaaag atgaaaatag ttctaatagt gatggtatat ctatgattat
     4441 attggtatct ttatttgttg ccttattggt tttactggcc tctggacttg taatctataa
     4501 tattttaaaa atctctgtta caaagaaaat taaagagtat ggatgtttaa gagcaatagg
     4561 agctgaacca aaccagatat ataagatagt tattttacaa atcttaattt tatgtactgt
     4621 tgccatacct attggagctg taattggaat tatatcttct aaaggcataa ctggaatggt
     4681 cactaatatc ttaaatccag atatacttct agcaaatgat aataaagaaa ttactgagct
     4741 tatacataaa aatacaacag catatatgtt cccattagta ttaagcacta atgtatcttt
     4801 gattttttca tttatttcag cattaccatc agcaatatat gcttcacatg tctctcccaa
     4861 aattgctatg gttggtagta ctaccaaaat caaaagaaaa attaaaagag aaaaaacaat
     4921 aaaaaacttt gaaagacatc tagcatggct aaacttaaaa agaaataaag gaagaactat
     4981 aataactata ctatcattat ttatgagtat aacagtattc gttgcactga gtgagttctc
     5041 taatgtatta gatgtatcta ggtcagtaag taatttaaaa gaaggagatt tttccttaac
     5101 aaatgaaata tctggttttg ataaatcaac actagataaa ataactaaaa tgaaaaacgt
     5161 caatcgcact tcctttataa agtactctga atacaaacag ggggaaatag atacagatat
     5221 aaattttgaa aatagtggtg agatgttaaa aatcattgga attgatgagc aaactttaaa
     5281 agacttaatg ccttctatta cagattctat actagaagac tttaaaaatg gttctatctg
     5341 ttttataaaa aatccgattg ctatttctac accaggtgta aaaactagac atacaaattt
     5401 aaaacctaaa gatagtatca caatcaacaa aaaacaacta gatatatata gtacagtaga
     5461 taaaatgttc tttttacaag gtaatggctg gataaatggt gttgatgtta ttgtatatga
     5521 ttctgtttat aacaccttga caaataaaaa caagttcaac cagataaata tttatgctaa
     5581 agataaatca aaattagaac aaataagatt gtctattgaa cagatttgtg aaaataatcc
     5641 tggctctcat tggatatcat atattgaatc agataaacaa cttaaagaga gttttaaaca
     5701 aatagaattt ctagcatggg cagttatatt atttgtggga ttgattggta ctttaaacat
     5761 cattaacaca actcatacaa atataaatac taggactaat gagattggag ttaaaagagc
     5821 tattggtatg agtaatagta gcttatataa aatgttttta tgggaaggtg tttattatgg
     5881 aatttttgct gctatatttg gttcaattgc tgggtatgcc agtgctatta ttataaacat
     5941 ggcaactata gaaaagcttg atttcactaa tataccaatt acttcaattt tacaagctac
     6001 tattatttct gtacttgcat gtattatagc tactcttatt ccgttaagaa aagttaaaaa
     6061 gatgaatata attgactgta tagatatcaa tttataacta ttttacagat aataatataa
     6121 attaaattat caatgactgt ctctattttc tttcaaatct aactaataaa tctttttaac
     6181 tataatataa gctttaaaaa aggcctatct caaaatagag ataagccttt tttcattgtc
     6241 gaaataatga aaatatattt aacttcacaa aaaaagagtg cctcacgaat taaaaatttc
     6301 gctttgagac actccccttt aatttaaaat atatagttaa atgctatgcc aaaatttcac
     6361 acatgctcca cctgattttg aattttctat tttaacagaa cctccaagct gttccactaa
     6421 ttgtttgaca atgtaaagcc caagaccaga atgtccacct tttgtctgcc tagcttcatc
     6481 tcctcgataa aatttatcaa gagccttttt taaatcttta gagctaaacc ctctaccaga
     6541 atcacaaatc tcataagaaa tacaattttt ttcatccttt acagtaatac taatattacc
     6601 acctgatgga gtgtattgca gactgttgga tatgatatta tcaaaaattc gtgtcaacct
     6661 atccacgtcg atttgaattg gggattgtat attcccctgc attttcaata taagttcaac
     6721 ttccttttga tgagcctgta attcatagtc ttgcaccttt tgcctcaaaa attcaggtaa
     6781 atttatagga acaagattta attgtatatt tgacttttct aagtcagacg tatattgcat
     6841 cttttggaca agagcagcac ttttttctgt attttctctt atcactgtaa gatacctatg
     6901 aagtttttca ttgtcatctg tattattacc aataagtgca tctgtatatc ccaaaataat
     6961 agaaagagga gattttaaat catgtgctag agcttccacc atttcaacac gttcctgctc
     7021 catcttccac tgtgcagata aagagccttt taattcgtct ttcatttcag aaaatgcact
     7081 acaaagttta ccgagttcat tatctgaata atagtcgatt tcaaaatcaa ggtccttttc
     7141 ttttatcttc ttggacgcat caatcaatag atgtaatggt tgattgatat tctttacaaa
     7201 gcgcttagaa agccatctag taaaacccac aatatataga aatggagaaa acagagccat
     7261 tataataact gcaaatacaa atctccctct aatatttgca aaggtgattt tcacttggta
     7321 gaatagaata acagctccct caatctttcc attatcccct ttgattggaa cagtatggat
     7381 ataaccacct ttacgaacag ttttattgat aaaattatta aataattctt cttttgtttt
     7441 aaatagtttt ttggggttag taccatataa aatattgcca ttattatcca caacttgata
     7501 tagcatatca tccccacgaa tcgttctttt taaaccttct tcattcgatt gtgataacaa
     7561 agttatgttt ttttcattga tgtatttttc aattctagga acttgtcgct cataatagtt
     7621 tggtggatag atatccttat ttaaggaatg aatatacaaa tagagagcaa atacataagt
     7681 aatcactgtg gcaactatac tggctattat aataaaaata aaagtatgtc taaattgggt
     7741 aataagggat tgatttttca tttaataacc tctctctctt tacattttat tccacttata
     7801 cccaataccc caaactgtgg aaatatattc tttttctggt gtcacagcag aaaacttagc
     7861 tcgtattttt tttactcgtt caacaacagt tgaggaatca ccttcagaat cacaccccca
     7921 tactttttca tagatttgtt ctcttgagaa aacttgtcct gcatggagtg ctagtaattc
     7981 aacaatatca tactctattt ttgttaaatc aatattctta ccatcaattt taattgcacg
     8041 ttccttcata tataagcata acttcccaaa atacaaagta gatctcttgt tctcttcatt
     8101 gatatactgt gaacgctttt ccctacgcag attagcttca atccttgcca taagttcacg
     8161 cagtccaaat ggctttgtta cataatcatc ccctccaaag gtcaaccctt tgattttgtc
     8221 ggtttctgat tgctttgcac ttaaaaatat aattgggcat aaaacatcat ctctaattgc
     8281 ctgacatacc tcaaatccat tcattttagg catcataata tccagtataa taaggtcagg
     8341 ctcctgtttt gataactcta tacctgcctg accatcataa gctatcaaca cttcatgtcc
     8401 ttttgccttc aattcatcct caagtagcat tactaggtcc atttcatcat caataatcaa
     8461 tattttactc aatataacac cacctttctc ttatgggaag gtcagtatta aaacaatatt
     8521 cgtctactaa taacttttcc ttccctccca cattttaaac caaataacgc ctcctatcaa
     8581 gaatataatc aaacctattg ctactactat aagctctata ctagctgttt gcatcacttt
     8641 agagtaaaat gcctcagttg aaatagttcc agcttcttta ataaaataaa ttcctggaag
     8701 catacctagt tttactggcc atgtccatgg aacaaatgcc catacttttt caccaacgct
     8761 agttaatcca agtattgctg ccatcaaaat tccaccgata ctaataccaa tagatgcacc
     8821 cataccccat gcaaaactca cccaaagatg aatagctaat agtggaagtg tcccaatcac
     8881 cacaagtcct gctgctgaca ggaataacca tattgttgct ccagatggca ctgcaatact
     8941 cattcctatg caaagtatca acgtagcaat aaatgtgcat atagttaggc aaaacagaag
     9001 aaaaagaaat tttcccagat ataatctaac acgagaaata cctacacata aaaaaccatt
     9061 aaaatttcca gcaagctctt cctcctgcac tataaatcct gctaaaatac ctactccaat
     9121 aggaatgatg aatacagtcc atactgtaaa aaatccttca aaagcaaatt cttgtgtact
     9181 tccagattta gttgtgagat aaaaaaccac acataatgaa aaaataatag gcatacaaag
     9241 agtaagccac tggacagctg tcctctttgc tcgaagccat tctgatgata atagctttat
     9301 catttgtcat ccctcctatt gaaccaagct gcagtaagtg ctgttaatac tataaacaca
     9361 accaaagaaa caacaatccc aataggaatc actgaagtat tcaaaagtgg gtctccagct
     9421 tctaatatag taccgttagg atgtatgttg atgattgggc ataccattct aactgcccaa
     9481 ctccaaggta ctgcaatcca aaatggtttt gtcgcagcaa ggacaccaaa tatcatacct
     9541 aaagctccaa ctcccatact taggaacata cctttccatg tagctagcca tagctgaagt
     9601 ggaagtatag caagagaaac aacccagcat acaatacttg cccctataat ctgtccaaaa
     9661 ggaactggac cagctttcaa tattaaacca gttataattg ttacgactat taaaactagc
     9721 gttgaaataa aactgtagac tgccatagct ataactttat taatccaaag tgtcatagga
     9781 gacacattgt gtgtacgtaa agcacgataa tttccagctt ttttttcctg gaaagctacc
     9841 aaagtagcaa acaatgcaaa tccaataggc agaaataaaa atgaccacca gttaaaaatt
     9901 aatgctaaaa gattttgcca cgcccaagaa gttgtttgag ataatggatt ttgcatgagt
     9961 gttgattgca ttataaatgc atatgctgca aaagacactg ggaaaaaaac aataagttta
    10021 cccataaatg ttcttttata ctttaaaagt tctgattgta aagcattcat ctgttttgtc
    10081 ctcctttaag aataacatct gtaaaaaatt cttctagatt ttcattaggg tcaggcattc
    10141 cttgaaataa tagtcgtcct ccatttataa ttcctatatt atctacaact tgagcaacct
    10201 ctgatagaat atggctagaa agaattacag ttataccttt ttttggaaaa gaagcaatca
    10261 attcacgcaa ttcttgaatg ccaaaggggt caagaccatt ggttggttca tctagtatta
    10321 acaactctgg gtcactcaaa agtgcaatag ctattcctaa cctttgtttc atacccatag
    10381 aaaattgtga agctctcttt ttacctgtat ttttcaaatt tactatttcc aacacttcat
    10441 aaattttatc tttaggtatt cccaatattt ttgtatgtac taacaaattt tcctctgctg
    10501 taaggtttcc atacaatgca ggggattcta ttaaagaacc tatttttgca agagattctc
    10561 gtttccaagg ctttctatca aaaataatac gaccatttgt tggacgaaga agaccaacaa
    10621 gcattttaag tgttgtggac tttcctgctc catttggtcc taaaagtcca tatatactat
    10681 ttcgttcaat ttgtagagaa acattgttaa ctgccatttg ttctccatat ctttttgata
    10741 agttttttgt ttcaagaatt aaatctttca tattaatttc tcccttcttg taatacctta
    10801 aatctgttac taagagaata ctaacatgaa aaaataacaa ttctataaca cctactaatt
    10861 ttaataaaat ttaaaataaa aaatagagtt accaatttta ttttggcaac tctatttaag
    10921 tttatgtttt acagttatat tagatttaaa cttagtttca ttatcacact aacttcaaat
    10981 ttactctata atctttaaat caaacactaa tttattaatc aaacactaat ctattaattt
    11041 atatttcttt agacatctaa tatagtttgc tactaaataa taattattta tatcatattt
    11101 atctaatcct ttaaaagaag ttatttcatc attaagctca tcactacctt ctacaaaaac
    11161 acaacctgtt tcaaattcca tacctacaat aatcttacag ccactataaa tttcctcttg
    11221 agtatcgtac aatttttttg catttccttc atttggaatt gtaaatacaa taggttttaa
    11281 ttctttatca ctggaaatct tttgtgaaaa aggcttgttt aacttaatat tattgataac
    11341 tacatgttta aattcctctg aagtttcttc tactattaca tattttcgct ttaaaaactc
    11401 tattatttct aaaacagact tagaatttac ttttaaaata cttttatatt tttcatataa
    11461 agatttccac tgttcaattt tttcctgtgg tatttcttgg atatgtgtac ttattttttc
    11521 ttgagatatt tttttcaatt aaactctcct ttcaatactt aaatatcctt atgaatctca
    11581 catgactaaa gttacaaggt ttctacttaa tagaattttg catacttaaa tatgtcttat
    11641 taattctcta caggatatct ctgtaattcc tacgattctt acataagtta cttattttaa
    11701 attgttatta gttagattct aaaaattatt tattttatca ctaatataat actcttaaaa
    11761 actttacaaa tcaatttaaa tttgctacct tttatttata acaacttata gtacatttct
    11821 cctatgcttt tcaatatatt acttttaaaa ggaggttttc attatgattt taaatagagc
    11881 tgagcttttc cgaggatatt ctttaaatag atttaacgag atatgcaata tattatcagt
    11941 aaataaaatt aaatataagt ataaagtgaa aaataatgct tactctaaaa aacctttttt
    12001 taatgaaata acacttggca atttaggtca aaaggaagat ttctcttatg agtacttaat
    12061 ttatgttcat aaagacgatt atgaacaggc agagtctcta ataaattcta agttgcgata
    12121 gattatttat aaaacatgac attctgttgt catgttttat atgttattat actttcaaat
    12181 atgctactat attttcaaaa ttgtgtgctt taaaactagc tttttatagt aatttaaagc
    12241 ttaaaactaa ggagactcgt tatatgaaaa taaatataaa atcaaagtta agtgaattta
    12301 ttaaacagaa caatcttttt gatgacagca gaataatatc ctaccttcct aatataacaa
    12361 tagaaacaga caaaataaaa actataatga ttaatgaggt tgtcccaact aacaccaatg
    12421 acgattttta tagtgtagat aaagatgctg attatttaaa aactactatt cctttatttg
    12481 acagtgcagg aataaaagta tctaacataa atgatatcct agatatgggt atctatataa
    12541 ctaatgcagt aaaacttcct aaatcagaat atacaataac tcgtgataca attaaattac
    12601 atatgcctat tttagaagaa gaaattaaat tatttaaaaa tctagaggta gtaatgttaa
    12661 tgggagatgt tgcaaaaaaa tcttttaata tgattactaa aaagcatata aaaaagaatg
    12721 tcatcccttc catttcaaca tataaacttc gtaataatga actttattat gataatttaa
    12781 gagtatttcc atcatatatt atgactggtg gaaacatact gattgaaaaa tcaaaatttg
    12841 aaatggcatc agaagatata aaaaaaatgt tcgagattat aaacggttga tattacaaaa
    12901 taataaaaac taatataaaa taaaaagtgc ctcaaaaact tctatttatg aggcactttt
    12961 attttatatt agtgtaacta tatactatta acataattta ttatttatat tgttcttttt
    13021 aatctctatt ccatttagtc taggtaacat tactctttac aagattactt tatatttctt
    13081 ttatatctct aacgttttta tttaataaaa caattattaa tacaagtcaa atatatctat
    13141 ttatctaaaa atatatcttt tacagtaata tctggatggt gatacttctt cctattcgtt
    13201 aaaaaagatt tttttacatt aatagcctct tttggacacc agtgaaaaca agctaggcat
    13261 ctttcacact cattacccca ctttggcttt ttatcaacta tacttatatt ctctacagga
    13321 caaacccttt tacatgtccc acatccatta catttatttt ctgcaaaaaa tttagtagtt
    13381 gcaatttgca tattcttacc tactaaacga agtacaacac tatctatatg tgactttaaa
    13441 ttgttttttc cttctatatt tccaatctcc aagttattga tacattttgc aatctcagct
    13501 accctattag tctgattttt caatctttct tcttcaacat ctggaggtgt tatcaaagca
    13561 tttccaggca tattgattaa aaaaccagca gataattttt tccccctttt tttaagtaac
    13621 ttatctatag taaataaact atgtccagct accccattac aagtggcaat ggcatatata
    13681 tgagtattaa tattaaaatt taatttctct acaaaagact ttactatatc aggaacatca
    13741 gcaaaataaa caggaaaaac aattaccaat atatcttcat ttatgtctat attattttct
    13801 tttctatgta tagctaaggg tataacttct ttacttccat ttaacgtttc ttttaaaata
    13861 tttgaaacta ctagcgaatt tcctgttcca gaaaaataat aaatcttact attcattctt
    13921 atccctcttt ctatatttct acatcatcaa tagttaaaaa gtctcttaaa ataggattta
    13981 tatttacttt ccaattctat ccaaaatatc agtaaacaaa ggcaattctt catcagagat
    14041 agatttaaat gtctctgttg catatatact tgcaatttca tttaatggtt cttctaattc
    14101 tctacctaaa tctgttagga atatttgaaa cttacgtcta tctttaacat ctactttacg
    14161 gacaattaat cccttatttt tcataacatc taagattctt gtcgtattag atttatcctt
    14221 acatgatttt tcacataaat ctatctgaga aattccatct tgctcccata gaaaccttaa
    14281 taacgcataa tggtcagttg ttatatcata tccattttgt ttaaacatct tttttaaatc
    14341 atcttttact tttaacgaag tacttactaa cttagttgta gcttcatata gtttctcaaa
    14401 atctttcatt ttacctccat ttaaataatc ccttgctcct cccatctatc aattattccc
    14461 ttatattgct caatatcttt aaaagccatt ggctttaaaa cagcatttat agcaataaaa
    14521 ttatagattt gagaaagaac aggtggattt ttcttttcct ctatgcaata aataaatttt
    14581 ttagatacct tttgtatctt tgattctact ttttcagtta taggattttg attaaatcca
    14641 gtttttatag ataaactacc acaaacataa cctcccgaat ctaagaccat ggattttata
    14701 aaagaaattg cttttgaacc acgtgcattt tcatatgtag taactgctat acatggctta
    14761 cgatacaata atctttcaag catcatatgt gctctatctg taaacacctt gaaaagtcca
    14821 gttacactac ttccataagt tgggctacca aatataacac catcactctt agtaattata
    14881 tcatgaatat actctacttt atcattttga ttgatacact tgcccatttt ataacaattt
    14941 aaacaaccaa tacaatatga tggatttacc tcacttaaat caaaataatt aatttttaca
    15001 tctggtatta atctttctat atctttatac aagtaagtta aaacttttga tgtagctcca
    15061 ttttttctag ggcttccatt tataactgta attatcattt ctatatctcc tttttagttg
    15121 ttatatcaac aataagtata tcaactatat ttttagctgt caatataaac catttattta
    15181 aagccatact tacacagctt aaataaaagt tttatttaac tacatgtgct tatttaatca
    15241 actataaatg aattattttt aataattgac aaactaaatt aatgataata tactttatgt
    15301 attacaaatg taagtagaag gaggtatagt atgaaaaagt taccacatat ttcagaagca
    15361 gaatatcaag ttatgaaaat tatttggaaa tatgctccta tcagcactac tgaagtaatt
    15421 gaaaagttgg tagaaacaag tacatggagt cctaaaacaa ttcaaacaat gttattgaga
    15481 ttagttaaaa aaggagcttt aacatatgaa aaaaatagca gagtattcgt ttatactcca
    15541 cttgttaaag aagaagaata cgtagctaga gaaagtagtt cttttttaaa tcgtttttat
    15601 aatggtactc ttaattctat ggtactaaac tttttagaaa atgataaact atctgaagat
    15661 gatattgagg aactgagaga aatcttaaat aaaaggacga ctaaggggga taaataaata
    15721 tgtatgcccc attctttata cgatttttac tcagtacatt aaccttatca atattaattg
    15781 ttgtaatttt acttgccaaa aaagtattta aaaaacatat ttctgcaaag taccaataca
    15841 atattggatt tatattgctt ataatgttaa taattccatt tgttcctctt aaatatatta
    15901 atcttggaaa tgtatttaac tatttatata cttttaatca acttggaagc agtactaata
    15961 ggacttctat gataaaaaat agtccaaatt ctgtaattaa taactcaaat gcattaaacg
    16021 atttttcagt atccctaaac caagttaatt ttgatagttt aaatatttta ttactaatat
    16081 catggataat tggaatttta atcatgatag ctattactat tcattgcaac attaaaataa
    16141 aaaatatgaa aaaatcaata caaattttaa gagatgatga gatagtattt atatttgaaa
    16201 agtgtaaaaa agatattggt ataaataagg attttatact atgcaaatca cctattataa
    16261 atacacctat tacatttgga tactttaaac cacacattat attaccagat aagtctatat
    16321 ctaaattatc tttaaaagat ataaaatata tcttacttca tgaattacaa cactttaaaa
    16381 ataaagatat actgataaat tatataatgt gcttccttca aatattatat tggtttaatc
    16441 ctcttgtatg gtatgcattt aaagaaatgc gtatagaccg tgaaattgca tgtgatattt
    16501 cagttttaaa aaagcttgat aaagatagtc atatagaata tggacaaact ctaataaact
    16561 ttgtggataa tatttcaaga ccatcaaatc ttacacttac atcagatatt ggtgggtcaa
    16621 aaaaacaaat aaaaaagcga ctattgaaga taatggattt tcataatgaa tcaaatacgt
    16681 taaaattcaa aagcatttta atttttacat tgatagcttt aattgtattt ggaaatattc
    16741 ccaaactatc tattatggca gatatcaacg aaaaatatag acctacaaat gaacaaactg
    16801 tgtacgagga cttaagtcag tattttaaag gttttgatgg aagttttgtt atgtatgatt
    16861 taaaatccaa gcaatatcat atttacaata aagaaaaaag taaaacaaga atttctccaa
    16921 attctactta taagatttat agtgccttat tgggattgga aaatgggata ataacaagaa
    16981 ataattcaca tatcccttgg gatggaaagc atcattctaa tagttcttgg aatgaagacc
    17041 aagacttatt ttctgctatg gaaaactccg taaactggta ctttcagact ctagataaaa
    17101 aaacagggct aaaaaacata caaacttatc ttaggcaaat tggatatggc aattatgatg
    17161 tttcaggtgg tatctcaaac ttttggctag aatcttcatt aaaaatatca cctattgaac
    17221 aggtagaatt acttgaaagt ttttatacaa ataagtttaa atttgaagat aaaaatatag
    17281 aaacagtaaa agattcattg ttaatatcaa aaaaggatgg ggtatcactt tttggtaaaa
    17341 caggcactgc caatataaat ggaaaagatg taaatggttg gtttataggt tatgtggaaa
    17401 aaaatgaaaa tacttatttc tttgctacaa atattcaaag caaagattat tctaatggta
    17461 gttcagcatc taagataaca ttatccatat taaatgacaa aaaaatctat aatccttaat
    17521 atatttactg tacattctaa aaaattcaca taattttcat attaattatc aattaaattc
    17581 atatacaagg cttattatat aaacatagaa gatagtaaaa ctagcttaaa aacataatat
    17641 aataccctat acaaatagtg gcaactctcc ctcacatcct caattgttgc cactatttaa
    17701 ttttttgtat gaagttaaat atattttcat catttcaaca atttgggtgc aatacaatct
    17761 ctattttgaa acaacctctt taattgtttt agagtttatg aattatttct catactttaa
    17821 aaagattgaa ttactcttga taatcttctt ttgtagtcat tacatatcac ctactactca
    17881 acacatattc tttgacctat ttttacatcc ttgtttatat ccaaaatatt taccttctta
    17941 ccatctggaa acaataccat tgtcaaagta gactttccta attcgtgtat tctctttaaa
    18001 tcaacattaa caagaacatc tccctgttta accatatctc cttgtttaac agcaacatca
    18061 aagccatctc catttaatgc aactgtatca attccaacat gaattaatac ttctccatta
    18121 gttcctttaa tcccaaatgc atgtccactt gaaaccactg tttctaattt acctgaaata
    18181 ggagaaacaa tttttccatc tataatatca atagcaaaac catcacctaa cattttttta
    18241 gaaaatgttt catctggtac attttctaat gcaacaattt tccccttagc tggagacaca
    18301 aagttttcat tattttcatt aaacactaat tcttcgtctt catcttcatt tatagcttct
    18361 ttaggcactc caaataaaca tgttacaata aagcagaatg caaccacaaa tattgatact
    18421 aataagtatg tgattaaatt atatccatca tagatataca ttaaataaga aggtaaaact
    18481 gctaatccat atgaattaga ttgaatatgt aaaattgata atattgctgc tcctaaacct
    18541 gaagatgcac acataataat caaaggcttt aaattatatc gaagctgaat cccaaataaa
    18601 acaggctcac ttataccaaa aagttgagat agcattgctc caaccgcagt ttgtttaact
    18661 tgattatttt tagatttttt cataaatgct agacatgttc caatattcgc aaatccatac
    18721 attgcacaaa gagtaataag ggcattaaat cctgtatttg ctaatagtga tgtttcaatc
    18781 attacataag tatgatgtaa gccagtaata acagctaatg gatatgtaaa tccaataata
    18841 aatcctccaa ttcctaaagg caaatcaatc aataagctga tgatatcaac catttttaat
    18901 tctattgtat gcattatagg gcctacacct aacaaaataa ctaacattgt tattagcata
    18961 acaaaaaatg gtgtgaaaat aaggtctaat acattaggca tatgtttacg gaaccatttt
    19021 tcaagattag ctccaataaa tgctgtaaca atagctgtta aaacacttcc ttgacaacct
    19081 acaataggaa taaatccaaa tgccataatt gcttcaattt catttgatgg gtcagcaaca
    19141 gaatatgcat taggaagtat tggggaaact aacataagtc caataacaag tccaataaca
    19201 ggtgttcctc caaatacttt aaatgctgac catacaataa ttgctggtaa aaaagcaaat
    19261 gctgtttctg taagaactga aacaagtgtc tgaatataca atggaatatt tgcagaagac
    19321 attcctaata atccaagtac attatcatta aacaaacaac cctttaatcc caagaataat
    19381 cctgttgctg caataactgg aataatagga acaaatatat ctgctagagt tctcataact
    19441 ttcttaaatc cagtttcatt atttttaact aattcatctt gttctttttt tgataaagtt
    19501 tttaatccca ttttttcaac ttctgcataa actttattaa ctattcctgt tcctaaaata
    19561 atttgatact gtccagaagt aaagaaaacc cctttaacct catcaacttt ttcaactttc
    19621 ttatcattaa taatttctct atctttaacc atcactctta atcttgtggc acaatgtgta
    19681 atagaaataa tattatcttc accaataatt ttaataagct catttgctat tttattatat
    19741 ttattcattt ttctacctcg ctttcaataa tactgcatga aatggtttca aacacactgt
    19801 tttatttaat agatttatat catctaaatt ttggaataca atatcatatt tttcttctac
    19861 tcttatttct acaggtttat cagaaaaatt ataataacaa tcaataacta ccaaatctaa
    19921 ttgccttcta tacgcaataa catcttcact ttctatatca ataggttcta ttgaaccata
    19981 aatcaaacaa tcagaatact cactatcttg tctcaacttt atcatctttt tataaaattc
    20041 taaaatactc tctgaattgt taatctgact ctgtgcatta atatctttat aattttcaat
    20101 aacctttaac catggtgttg tatctgaaaa acctgcattt tctttatctg cccatggaaa
    20161 tggcgttctt gcattatctc ggcttcttaa attcacaaat tctaacgctt cttcttctga
    20221 gtaaccttca tctattgaac gatagtattg gtcaatgcta gataaatcat taaaatcatt
    20281 tatggtatct cttttaaaat ttgtcattcc taattcctgc ccctgataaa taaatggggt
    20341 tcctcttaaa aagaaataca ttgctcccaa catttttact gcatcatcat tcctttcgta
    20401 ttgcaaaaga tactttgttg tagctcttgg ttggtcatga ttttcaataa aattagctgc
    20461 ccatccatgt tcctgaacat acatttgact atctaaaatg cattttctta aatctggaat
    20521 tgtccatgaa attcttttaa accactcact accagaagca acatctaagt cagcatgttt
    20581 aaagtcaaag accattgaaa agaaaccatc ttctccaata aaatcaccta gctggtcata
    20641 gctaatacct gctgcttcag caacagtcat acaatcatat tttttaaacg tattttcttt
    20701 taattcataa agcatttttt ctattccagg ctgatttcta actgtttttg tacatttaac
    20761 aagaccatca acaccatcac tttctaaact ttgaaaactt aaattctttt tgataaatgt
    20821 aatagcatca actctaaatc ctgccactcc catttgagac caggtattaa tcatttggta
    20881 aagttcactt cttaatattg gattttccca attaaggtca ggttgttttt taccaaaaga
    20941 atgaaaataa tattcgtctc tattctctac cttttcccaa acacttccac caaaaacaga
    21001 cctccagtta tttggtaaat cgtttccttt tttaaaaata taataatcat gataaggact
    21061 ttctggattt tttaatgctt cttgaaacca aggatgttca tcacttgtat gattaagaac
    21121 taaatctaat ataattttga ttcctctatc tttactttct ttaattaact cttttaaatc
    21181 ttccatactt ccaaattcac ttgcaatatc caaataatca ctaatatcat acccattatc
    21241 gtccataggt gatttaaaaa ttggacataa ccacaatgat gtaataccta aatcttttaa
    21301 ataatctaaa cgagaaataa ttccctgtaa atctccaatt ccatcattat tactatcttg
    21361 aaagcttctt ggataaattt gataaacgat ttctttttgc caccatttct tttccataaa
    21421 ttactccttt ttatatgata tttcttcttt ttaattcttt ataaattcca ttatcgaata
    21481 tgtcctcaca tatagatgtc gctatatctt taagttgttg atgactattt ttcattgcaa
    21541 ttgtaacgtc tgatgcttga aacatgacaa tgtcattttg accatcacca aaacatatgg
    21601 tttctttttg agttactcct aatctttctt gtaatctctt aattgctttg cccttatgaa
    21661 aatctttttg aataatttca taatactgac tagaaatatc tctctgtgct aattccattt
    21721 tatcttgtag tatatctttc acttcgtcaa ataccttttc atttgaccat aaacaaattt
    21781 tatgtatatc ttgtgactta tactcttcaa tgttattttc atatgttatt ttttcttgga
    21841 tatgttgttt atttatacaa gaattagttc ctttcaactg attcattgtt tcaaatattt
    21901 ctttggcttt ttgattcatg aaaacttttt cctgactctc aatcgaaaaa gcaacttctc
    21961 tcttttttaa taaacaaaca acttccttta tcaatctttg attaaaagat tgattataaa
    22021 gtaattcacc atgatactga atgtaattac ctcctcctgc aatatatcca tcaactccta
    22081 atgataaaac atcatcttgt attgtaccca tacttcttcc agtacaaata acaacactgc
    22141 aatgattctt ttgacataaa cgtatagcat gctttgcaga ttctgggatt ccatatacct
    22201 catctctaag cgtaccatct atatcaagta ttaataattt gtacaatttc atcttccttt
    22261 cttactatca aaatcataat agcattcaaa aaaaaagata tcaatatttt gatatctttt
    22321 aaaaaatatg ttcattttta gcacaaattt tcattttcgg ggaatatgtt cactttctaa
    22381 atacatcttg tatttaatat aaagcagttc aattaaaata aaataaggtg ttgttacatc
    22441 gtagtccagt tgtttatcta cagaaataga tatagattga atatacaggt tttcatcaca
    22501 taatcttgct aaaggattac ttatcatttt tgtaatagaa accatataaa ttcctttcat
    22561 tctcatttct ttagcaaaat caataattgt agatgcatct ccattcaatg aaataaagaa
    22621 aatcatatca tctgttttta taaaatgatt aagtgcctga atcatatcat aaccataaac
    22681 atcatatata attttccctg ttggtaagaa aatccttttc atctctttag caactctttc
    22741 ctgagaataa ccttctccaa agacgattat acgatttgcc tttttcattc tttcaaacat
    22801 aatcgaacaa tccttcttct ctatatcatc tatcacctta ttataacaat ccattaattg
    22861 gtgtttactt tgatttttat attctacttc tgtttcattt aagcgtataa taactcttaa
    22921 ttcactatat ccaggtaatt gtagtttttg agcaaaacga gaaagtgcac ttgtagatat
    22981 atggtatttg tctgcaaatt catctataga aagtttaata caatcttctt tatgattaag
    23041 aatacactca cagatatact tatcatttgc tgtaaatgaa ttataatatt tattgaaaag
    23101 tccttcaatt ttcattcttt tctccttcta tactctatgt aattcatatg aagcaaatct
    23161 aatagagcat aaaatgctcc tacgacttca taagatatag cttgtaatgt atcaagtttt
    23221 tgcgtttcaa catacagatt atactcacaa agtgttgcaa ttgtattatt atcaagccta
    23281 gttaaagaaa tcgtatgtat atgattgttg ataaattttg caatttcaat tcctgctttt
    23341 gtttctccag acaaagaaat gataataaat aaatcacttt tttgaaatga ttctctcata
    23401 aattcaattt cacctatatc aaataagtca ataacacatt tacctactga aagaaatatt
    23461 ctctttattt cttgagctaa acttttctgt tcattacctg tcccataaat ataaattgtt
    23521 tctgcatcat atattatttt acaaatatct tgaaatgata ttttttgtaa ttcattcatt
    23581 aatagtcgat aattctcgca aacatcatga aaattattat cattattaac aaattctagt
    23641 tgattatttt taagtaaata ctttaaatca gagaatgaat ctaatccaat ttttctacaa
    23701 agccttaata aagtagctct agaaacatta cattcatttg caattttttc acacgaaaaa
    23761 ttacggtatt ctctttgaca aatcaatatt tgtttaataa taattttatc attttcattt
    23821 aaattatcat aattttggtt aactaacgat tctaatctca ttttttccac tccctaaata
    23881 ttattttaat atacattagt tgctttttct agggaagagt tttaaataat aaggataaaa
    23941 taatcatttg attttatttt tattaaaata aaaataacct gctaaaagca ggcaccaaaa
    24001 agtacttatc ctttcgaaca tgtactttag atattgcttt cttcagaagc ttcatctctt
    24061 catactctaa aatagagttt tttctaactc aaaagtgttt tccatggtag aaattacttt
    24121 aattcatctg ccatttactt tttatcttat ttaataatta ctattacttg aatatttact
    24181 tctttaagta aatttcatag ttaacaaagc taaaactatt attatagatg ttaaaataca
    24241 aaattgtaag tattgttata aatatgctta cagctacaaa tatacatgat atattaaaaa
    24301 gaagctgtaa attattatca tacatacaag caactatgcc tctattaatc catgttaatt
    24361 tttattattt aatacaagga gttccatgag acatacatat ccattcatat tttaaattat
    24421 ccaattcgct tatagatttc atcatctttt tataatccca attccatgga ttaggataag
    24481 gaactatatt tccattctta tttttgacta aatcacctgc aaataataca tcttcaaaaa
    24541 tcatgcagac atgccctgga gtatgaccag gagtatggat aatttttata ttcccaatct
    24601 gcatattctc tccataaggt tcaacatcct taatgagcct tctggaaatt gcctttccaa
    24661 taaacttctt aaacccaggt ctatccttct ctcctataat aaaaggtata tcctctacat
    24721 gagcccatac ttttgctcct gttttttctt tcaataattt aatattagat atatggtcaa
    24781 catcatgatg agttaataaa atatgcttaa tatctgtaag tttaatgcca agtgttttta
    24841 attctttaat tatattttcc cccataaaag gaagacctgt gtctataaga ataatttctt
    24901 tatcaaacac aataaaacaa tttgagccac gagtactatc taacattaat acattttctt
    24961 ttatcttcat aaataaattc cccttatttg gtattttacc tatataatta attaataaag
    25021 ttataaatag ttataatact tgatattaat tttatttttt attaaagtat actataggta
    25081 tacaagtatt atatttacat aagtatacca tcagtatact tttttatcaa tagaaaggaa
    25141 taatttatat gtcagaatta tcaaaaagag ataaagaaaa aatacaaaga gaaaatgaga
    25201 ttattgataa ggcagaaaaa ttattttgtc ttaatggatt tgataataca actatgaatg
    25261 aattagcaaa agaagttgaa tatactaaac gcaccatata caagtacttt tcatgtaaag
    25321 aagatttatt ctttgctgtt gtcttaagag gatataaaag actttgggat aatgttaaaa
    25381 tagaaagtgc aaaaggcaaa acagggtttg aaaaaataaa actttcatac tttgcttttc
    25441 ataaatttta ctctgtagaa ccttctctat tatgccttat gggaatgatt ggtattgtaa
    25501 actctgaaaa atcggatact gatatgctgt ttaaagaaaa gtttttttct tttaataaat
    25561 ttatgtttga tgaaatacaa ggaatgtttg aaattggaaa aaatgataga agtattaggc
    25621 atgatattga aatacctact cttatgtatt catcaatatt tacacttaca ggatttttta
    25681 atttactatc tgtaacagga aaatcatacc tgaacaactt taatatagat gaggaaaaat
    25741 ttattgaaac aactcttgct cttttaatag attcaattaa agcataaacc taagtttata
    25801 aattaaagtt tatatactta aatgataatt ttttctatct ctaatataaa aaatattaaa
    25861 cataaaacta ataacttagt taatggaata actaaattac tgaaaggatt aaaagtaaat
    25921 ggctaaaata cttcttttag aagatgatga tacaattaca tttggaataa aaacttccct
    25981 tgaaaagaaa aatcacactg tatgttgtta taatacgatt gctgaagcta aaaaaaattt
    26041 tgataaaact attgatttaa ttctgcttga tttaaatcta cctgatggta ttggatacga
    26101 gttttgccaa tatgttaaaa gtatagaaga tactccagtg atatttttga cagtccgtga
    26161 tgatgaaaaa gacattatac gtggattaga tatgggtgca gatgactatg tggttaaacc
    26221 gtttttgctc tcagtacttc aatccagaat taatgcagtt cttagaagaa ctcattcaaa
    26281 tactaacaat actattacat gtgaaaatat aagcttgaat aaggttgaag tcaaagcata
    26341 tttagataat gttgaagtac ctttgacagc aggagaatat aaacttcttc taagtcttat
    26401 ggaaaataaa aatagagcta tgactcgtac aaagttacta gaaatgctct gggatgtaga
    26461 tggtaatttc gtaaatgata acactcttac tgttacaata aaacgtctta gagaaaaatt
    26521 aaaaaatcca ccttgtataa aaacattaag aggcatagga tatagaatgg aggacaacta
    26581 gtgaaaactc caagaaatat caacagtaca gttgttttta taatattctg cttaattgta
    26641 acttttttat tgagtgtaat tctaacaact ataacttctt ttaatgaata caatgtactg
    26701 tctaaggctc ttggtgcatc atatgagtac aatcctaagt ctacagaaag tatactaaaa
    26761 tcactgaaaa attctagtga taaggatttt aaaacaggaa atgaaatttt atcaaaacat
    26821 ggatattcta aaagtacatt ttggagtaaa aacttttttt attttctagt gatatcgttt
    26881 ggtataattt caacaatggc tacagttgta tatataatca aacatagaac aaataagcaa
    26941 aaactgagta gaataaacaa tcttactcaa tacttagaag aagtgaattt aggaaaagag
    27001 tctgtacttt tacgatgtga agatgaattt tctcaccttg aagatgagat atataaaaca
    27061 gttggggaac ttcgttgttc aaaagaattt gctttaaaag aaagacaaac tctttcagat
    27121 aatcttgcag atattgctca tcaacttaag actccaatta cttctatgtc acttatggca
    27181 caattacttt cagaaaattg tccagatgaa gaaatagatt atattaacag acttacaaat
    27241 caaatctcaa gacttgagag actagtttca tctttactta cattatcaaa attggatgca
    27301 agtactttaa tatttgaaag caaacttata gatgtacatt ctcttcttac ttatgcaatt
    27361 gagcctatag aaggttcttt aagagaaaaa catcagacat ttactatcga ggcttctcct
    27421 tcggtagaat ttgaaatcga tattaattgg actttagaag ctcttttaaa tattcttaaa
    27481 aattgtagtg agcatataga agatggtgga tatatagttg cttattatag tcaaaatcct
    27541 ctatatgttg agattatcat agaagataat ggtaaggggt ttgctcagga agaacttcca
    27601 tatattttta atagatttta tcgtggtaaa aatgctagta aggacagtat tggtataggg
    27661 cttgctctat caaagtctat tatagaacgt caaaatggta ctatacatgc agaaaattgc
    27721 cataatggtg gtgcaagatt cataattaaa ttttacacat aaaatgctgg attataggta
    27781 aaatacctat aatccagcta tatctatatt ctatgttatc tattgtttta gtaatatact
    27841 tctatttaca tttaaggttt tattaaacaa acaacttaac tatccctata cttgtaagta
    27901 gtcttaaatc gaaaccttta aataagttca aatagatttt gtttatattg tttcattttt
    27961 tatagattct actatatttt cattcttcaa ctgcttgtat cctgtcatat atgatgctat
    28021 tacaataaaa agtatcaata tagtaaaacc tattattgta gatataggtg caaatggtaa
    28081 atactctata aaatatattt catttatacg tagaaaagca tacactattc ctatttgcac
    28141 aggaatacta agtagtaatg gtgtaattcc taagaataaa ccttccaaaa taagcatctt
    28201 ttttatgcct tctggagata atccaacaga acgcaacata gcaaactctt gtcgtctact
    28261 tctaatattt cctgacactg tagcaagtac atttgataat ccaatcatag ccaataaacc
    28321 tgtaataaag cctactatta aattcatggt ctttattcca ctagcatctt gtttttcact
    28381 ttctaaaaca tcctcaatac tataatctcc tgaaccatag tctttattat aaatggactc
    28441 taatttttct cttactctag gaatgtcctc acgattattt actttaaata ctcctatcag
    28501 cctatttgaa cgtaacctac ttacttcatc ataattttta gatatttcct gcaccataga
    28561 cattggaact atttgcatta atgtatactt tgaactagta tctactattt taggaagtgt
    28621 gcttgtaata gctcccactt tcatgttgta agtataatcc cctttatctt cgtcatatac
    28681 tttttcattt aatttaatgt tatctcctat tgaaagattt aaataatcta tatatacttt
    28741 atttctacgt gtactacgat taatatcttc ctccttatta tatacaattg aggttggatt
    28801 ttttgtatca taaaataact ttgggtctac agaaatttgc ctacaatatt ctgtaaaact
    28861 cttatcatca agcccaatta tatttgaaat tatacgaaac tttccatcac gttctatagg
    28921 agagtacttt ttagatgaag ctatattttt aaatcctcca tttttcgcaa gttcttttga
    28981 agcatctttt tcagtcaacc aagtggaagc agacattttt tgataatata gtgaactttt
    29041 tacaccttct gtattatcta atttttcaat gaaagcagac tctacaggtt cactattttc
    29101 caaataaact gctatatctc tttggtcctt ataattatca cttccataaa cagcttttgc
    29161 tccttcttga ttggcaatta tatgcaaaaa acatgtaagt aataaaaaag ataatgttaa
    29221 agatattgtt gcagtacgat aagatttttt acgagcacgt agtgcgtttg atgcaagttc
    29281 tccttctatt ccaaatatct ttcctaatga atatttatgc gactttttga ttttaacact
    29341 atctccttgt cgtattgctt ctataggaga tatctttgat acttttcgtg ctggtataag
    29401 tgcagaaatc cagactgtta ctattgtaag taaaactgct ggcagaaaag caggaatacc
    29461 ataagtaaat accacttctg gaacatcagt atgataatta actgaattaa cataaactat
    29521 aagcctatta cacaataacc atcctaaaaa taaacctatt ggtaatggta taattgtcag
    29581 taaaaagcct tcaaacacta cagacctttt aatttgtttt ggtgatgcac caacactaga
    29641 taatatccct aactgagtaa gtctagcatt tgcagacagt gaaaacgcgt tgtaaatcac
    29701 cattacaaac acggctactg taaaaattgc tattgtaaga tacataatag gagttgaaaa
    29761 ttttgatata ctactcatag atgctttctg ttctggtgaa aagactaaca tctttaaaag
    29821 ataattacta ttgtatttaa gattgtattt tccatattca ttagtcttcc atcccaatga
    29881 tttagcaagt tttggaagtt ccttatacgt gtctcttata tttttaaatc gcaagtacac
    29941 agttatacta tcattaggtt ttatacttgt tttatcaaga tatccaagag ctgtatatgc
    30001 aggtattact gatgaagtag aaacatctac ctttcctact attgtcaatg tagcctctga
    30061 aattttttta aaactttccc ctttcgattt tggggctatg ggttcaagag gctttccatt
    30121 taggattcta tctcctattg ggagagtaat tttatcccca atttttacac ttggattatt
    30181 ctcaaaatat tgttttgata atgcaatttc attagcatta tttggtcgac gaccctctat
    30241 tatagtatcc ttttctggca tagaattcca atactctgat gtagcatttc tataaattat
    30301 ataatctctt ttctcatcat taattttgcc tacataccat tgtcctttta ccattacatc
    30361 atcaactgaa gaaaaatttt caattaatgg taagtcactt ccataagtat agtcaaacaa
    30421 ttcaccatgc cagttacctt ctttctcaat tgaaagtttt ataaaatctg tccacatggt
    30481 atacaacagt ccacacaaag aagacatcat ggttgtagta agtaatatag ctaacattat
    30541 agctatacta ctacgtttat tgcgtcgtac aaagtctaag gtgtattctt gaagaatatt
    30601 catttcttca ccaccttttc agagacaatc actccatctt ccatagttac aatacgattt
    30661 gcctctagtg ctattttctc atcatgagta ataagaagta ttgtctgatt aaatcgttta
    30721 tttgataagt ttaacaaatc tacaatttct tctgaatttt ttctatcgag attaccagtt
    30781 ggctcatctg ctaataaaat agctggtcta taaattaaac ttctagctat agataccctt
    30841 tgttgttgtc cacctgaaag ttgacttgga agatggttta atctatcaga taatcccaat
    30901 atagatacaa tctcagagaa tctatcttca tccacctttc ttttatcaag taacatgggt
    30961 aataatatat tttttctaac atcaagagta ggtataagat tataaaattg ataaataaga
    31021 cctacttttc tacgtctaaa aactgcaaga gcttcttcct ttaaacttgc tatttctgta
    31081 tcttcaacat aaatctttcc agatgtaggt ctatctactc ccccaagcag atgtaacaat
    31141 gtagatttgc cagaaccaga tgctcctaca atggatacaa aatcaccttt ttgaactgat
    31201 aaatttacat tattaagggc tgtcactctg gtttgatttg aaccatatat ttttgtgaga
    31261 ttttcacatt ttaatatttc catatacaat catctccttt atggttaaat tttagcaacc
    31321 taaaatgaca ttatagtgac atgaatacaa atataaatta ctattaaaaa tattgtttag
    31381 tttttgcaca ttatttaaaa ttataatata aaacaaagac tatgcaatat attttattta
    31441 tacttttaat atattacata gtctttaaaa ggtattaact ttaaatctat aggtattcaa
    31501 tttaataatc aatagcaagt attttatgta ctaaaggagt tttgctctat atagactcaa
    31561 tttagtaaaa ttatttaata aatattacag actataggta aataatattt acaaataaaa
    31621 ttaagtttat tcccttacac tataatattt cttaattata ttaattgcaa tttcctttac
    31681 ctttggccct gtaatttctt tattggaatc atctgattta atagcaaaat aataggtgtc
    31741 accttcacgc tctagcatac caacaaacca tgcatccaca cgtttatttt tttcatcaaa
    31801 tcctgttcca gtttttccat atacatttat gtttttatca tttacatcta ttttcataat
    31861 atctcttaaa atattaatat gctctttttt aaagtttgta tctccctcaa atatttttgc
    31921 cattgtttgc acttgttctt ttggagatat ttgtaatgaa gattctaacc aaaatccatt
    31981 taaatgtccc tttccatttt ttaaatctcc ttcccattcg ctaatatcgc aattcccata
    32041 ctttaaatca tctaaaatgt tttggacata tttagcatca actttattta tcaatttttt
    32101 gtagtaccaa acacaagact ccttaaatgc ctcttctaaa ctaagatttt tattccaatt
    32161 tttatttgga tactctgtac catcatatcc cataactgat tcttttgaat ttattacccc
    32221 tttttctaaa ccaattaaag ttgaaactat tttaaaagta gaacatggtg accttcttgt
    32281 ttcaattaat tctttgttat atatattata ttctttattt ttagtattat aaaaaatagc
    32341 tcctccacta atatcttcaa aacaatcact atagtcaacc atattaattt tttctttttt
    32401 gtttttatca ttgttttcaa ttttggattt atttaattca gacttattta attcagattt
    32461 tatataattc ttatcagttt tttgatttat atcgttatgt tttttcccta aataatacat
    32521 taccataact acaccaacca ataagatagc tatccaaata aaattctttt ttcttttcat
    32581 aatttatatc atctcccata tttaaattta taacctacta ttaaaatagt atacattatt
    32641 tgtaatatac tccaatctag gttctactta taagaaacaa gactaaatga atagctgtat
    32701 ctcttatatt ttcttcacct agtacaatta tgttgattaa acctattgcg aagataatta
    32761 ggtaactatt aataacatcc tgtcctcctt ttaatttgtg atataataat tgattccaca
    32821 tttaaaactt actattgtaa tatttaaata ttacaatagt aagttttaaa tgtcaagtta
    32881 ctttgggaaa gttattttaa ttgtataatt gttattacat ttataaaata tggcatgttt
    32941 aaaattattt tgctattggg aggattaaga taagcagtat tataaaagct ataaattata
    33001 aaatttaata tataatttat agcttttact tttacaaata ttgtttttat tgatttatta
    33061 atgaatttta ttgacaggtt atatttatat aagtaaacac ttacataata agtatataat
    33121 ctacatatct ataagttgag tcaattcacc agtatatgtc aacttaagtt tatgcatagc
    33181 tcgtgtacaa gcaatatata acaaactgcg gtcataatca ctataatatg ttttattatt
    33241 tactgaaggt acaataactt catcaaactc taaacctttt gacattttaa tagaagttat
    33301 ggatactcct tttgtaaagc tagaactttc agaagatatt aagtgaacac tatatttttc
    33361 tcttaacgca tcatatacag ctttagcgtc attatctgtc tttaatataa taccaagagt
    33421 cgcattatca ctctttttaa attcttcaat ttctattttt atcttattta tttcatcttg
    33481 ctcgttatta cattttaata aagctggttc ttcaccatgt cgttcaatag gctctaatga
    33541 agatatgtct tgaatctttt tggcgaaatt aataatctca aaagtagaac ggtaactctt
    33601 attcaacata actagttccc catcattata tagctgtttc atatcatcta gtgtatgagt
    33661 gtgattagga ttaaccaatt gtccaaaatc tccaagaata gtctttttac acttaaaaag
    33721 taagtttatt acagcatatt gaataggtgt gtaatcctgc atttcatcaa taacaacatg
    33781 tcttataatc ttatcttctt gtataccttc atatgcagca tgaatatata taaatgggta
    33841 tacatctgac cattcaagag ttttttttgt tgccatcaca aacatgtttg atatattcat
    33901 ttgtttaaag aaatccttat atagtgtaag agtatttttt attgtcaaca ttccattcaa
    33961 acttttaagt atacttttta cctttggtaa atcttcttcc ataatattat ctgattcaaa
    34021 tttataatga atgtcttcag ctaccttctc taatctcttc ttaactggat atctgttata
    34081 tgcctcaaat ctactctgta tccagtcact tttagctgta aaacttccaa aagtgtagtc
    34141 ttttggaata aaaatcttat ttggcatttg ttttatataa tcatcaataa gttttacaaa
    34201 atcaagtgta gatttaaaac gcactctttc agaccattta gcatcattta tttctaatgg
    34261 gtctttctca ctttcaaaat taataactct atctaattgt acctctgcta tattttcaaa
    34321 acttaattca cataatggct cttctccaag ctcaggaagg acattggaaa tatagtctcc
    34381 aaagacttta ttaggtgata ggataattac attatttgct gatattttat ctttaaatcg
    34441 ataaagaaga aaagcaattc tgtgtaatgc tatagatgtt ttcccagaac cagctacccc
    34501 ttgaataatc agtgtatcag ctttatcatt tctaataatt tgattttgtt ccttttgtat
    34561 tgttgaaatg attgacttca ttttttcatc tgaagtgtga ctaagttctt tttgaagaat
    34621 atcatcttgt atattaatgg aactttctag tgcatattct aattttccat ttttaatttt
    34681 aaattgtcgt tttcttgtta tttctccatc cacccatcct attggagcat catatccagc
    34741 ttttccgatt tcataatcat aaaacatact ggcaattgga gaacgccagt caagtattat
    34801 aagctcatct tcataatcaa atgcaaatct accaatataa tacttagatt catcatccat
    34861 atctttcagc ctaaaatcta ttctagcaaa gtatggagaa tctttgattt tattaatctt
    34921 atctcgaact tttaccataa aagccccata gttatctatt tgttttagtc cttgttcatt
    34981 ttgaaacatt tcatgtgggt ctatttcacc acgatttttt accatataca ttttagcgtc
    35041 catataatcc ttatcaagct tgtcaacaag attttcagct ttcttaaaag cattgtctag
    35101 cttgttattt atctctatta gatgtgcaat ctcatctgga aaaaaagtta ataactctgc
    35161 atcatattca cacatattat gtttagaatt agatgcaata atatcgcaat ttactacttg
    35221 tttattttca gacatttaaa tcctcctttg ttaattaagt acttacactg aatcattata
    35281 cttaattgtg ttttggagtt tttaatcatt cttgctattt aacttaaagg aagaataaat
    35341 ttttgagtag gatatccaaa ttccacaagc aactctgctt ctgcaaagcc caacttcttt
    35401 atcatatctc tatgtcctgt atcagctttg tcaccctctc gaaaggtagt aattgaaata
    35461 tctgtatcta tcaagaaatc ttcaataact ttcttcaaaa acacttgtgc aatgtctgaa
    35521 ttacgatact gtggatgtat tccaaaaaaa tcaatacttc tagtttctct attaaacacc
    35581 atatttgcaa tagcgacatt attaagtttt aatattaaag ctctttcttc taaaatatac
    35641 tgttctaatt gatatatata ttcttcctct tgtaaatttg gaaatccatc tatgactaat
    35701 ctcactaaat tcatccataa tggtatgtca gacatacttg caagtttaat ttctttttcc
    35761 aactctagta cattctttga aaataggtta tcttttttca aaacaaatct tagctgcaaa
    35821 ggataaaatt cttgttcttt cctatattta tttggagatt ttttatacat agctttaaat
    35881 atagaagtga atgcctgttg actttcatat ccagctatca atgcaatttc aataattggt
    35941 ttctttgaaa atgccaataa ttttgctgct tctgtcaact ttctccgctt tatgtaatca
    36001 tgcattgtta ggccaacaga agttgtgaaa gttctatgca ggtgatattt tgagtaatga
    36061 acagcattag caactatatc taaatccagc ttttctgata aatgctcctc tatatagtca
    36121 atagcagtta tgacattaat tactttgtaa gacattgcag cttccctcct tttatccatg
    36181 caacattata tcaattatta cttagttata tttaatgaat cttgctattt tataaacaaa
    36241 tttgttgttt aagttttcaa ttcataaatt taatatatta ctttttactt tctgattaaa
    36301 gactctaatc ctttaatttt caataatttt tcatttagta aatctaaaaa gttatttatt
    36361 attttcttct tattagattc cttttatatt ccttaattaa atgataaata tttatattat
    36421 acgactaaaa acagcttttt acaattattt aatatgatta caaacttacc ctatcttaaa
    36481 gtttaatggg gtcctgttat gaatcttttt attttaattt aagctcttca tcagtttatt
    36541 ttatatattg atgtatttga tttcatacaa aaaagagtgc ttcatgaact aaaaagttca
    36601 tttttaaaca ctctctttta tgtaaataaa tttctattct attttgaaat attataattt
    36661 gacttataat ctgttaatac caaaaataat aaattgtacg tctaagaata gattctaatt
    36721 agttgctcag tagtaaaatc tgtttttaac taatcctgct ttgtatgttg aacatattct
    36781 ataattgtac catctggatg tctcaatgtc atattatatc ctgtaggaac ttttttctta
    36841 tctcttacta caactgctcc attatccaat agataacttc tccattcatc aacagaatca
    36901 accataaaag tagattttgt ttctataaat ggtttaagag catcatcaga accagataat
    36961 agtaaaacat ttcctatttg cgctaattcc aacccaactt ctttatagga aaaacgcaaa
    37021 gaacattttt cttttaaaag attttcataa aaaggtatgg ctttttctat atcttgaata
    37081 taaaaacgtg ttaatatatc atatattttc atattttccc cctatatatc tatataatat
    37141 tacctctaaa ttataccact ctacaaatta tataacaata aattaagcat agccttttga
    37201 gcatcttaag tagaccttaa caatatattg gggttacatt agaatacata aattaaatca
    37261 ccttgatatt atttaggata aaatttatgt agtagcagta aatgttcttc taattatcaa
    37321 ctaacaaacg ataatcttta actttctata taatgggtaa catcattttc gtaagatatt
    37381 cactagctgg acgattgata tcatttaaat actggtaata tatatcgcca tgaagtttat
    37441 atccattgct atttgcccat gaaaataagt cttctaaagt agggtcttgt aattcataag
    37501 gtcctaaatc aattgcagtt attattttct gtgcaggaac aatcctttgt agaatctcat
    37561 ttttcccatc tatataagtg gctacaggaa accctacttc aacatccaat tttgctaaat
    37621 ccatgttatg aaaacataca atcggtgctc ctgaagataa aattcctctc tcctccagat
    37681 atttagaaat tttttcaaaa ctttgttttg aaaactctga aaactctacc ataaagtcaa
    37741 ttgtttttcg tattgccaaa aaacagtact cagattgttc agctattgtt atattggata
    37801 ttctagtcat tttattcctc cattacttta ttttcatttc taatcttttt ataccaaatt
    37861 atacgaaaat ttactgtgta actaattatt tcatatgaat ataaaacata acctccttcc
    37921 atttcttata tcgaaagtat agcaaaaata atctcatcat tcttgtcaca cagtgctctt
    37981 ataatcagaa catacttaaa agacttgcct aatttttagg caagtctaaa agaaatcttt
    38041 ttctataaaa aatttataca aacaattaaa agatcaaaat atagactaat ataaaactat
    38101 aaatttacta atctgaacct cccatggcta aagtcacaag attactgatt tatagaattt
    38161 cgcacactaa aatatgtctt actaattccc cacaggctac ccccctagtt cctacgttct
    38221 tacataagtt atctattatc aaacatcaaa atttaaacaa gtttcaggtt catcttctat
    38281 tcttgctatt ttaaacttac atgcaattaa tactaaagat actattggat ttataatatt
    38341 taataaagcg tatggaccat atgttagtgc agatacacct aatgttgctg ttatatacgc
    38401 cccgcatgta ttccatggta ctaatggaga ggttaaagtt ccactatctt ctaaagctct
    38461 cgacaacatc ttaggatgta tacctctctt tttatattcc ttattataca ttcttcctgg
    38521 tataactata gatagatatt gttcacctgc tattatattg gtaattgtac aagttaccat
    38581 agtacataat actgttccaa atacaccctt tgctaatctt agtaaagaag atgctactac
    38641 ttcaagcata cctgtttttt ctaaaatacc tccaagagat aaagcgcata ttgttaagga
    38701 tacagtggac atcatattca taatcccacc tttagataat aaagcatcta cctcctgtat
    38761 ttgtgttgac gaaacatatc cattttgtgc tgcatctaat atcgtattca tagattctcc
    38821 ttgaaaaact actgcaaaca atactcccaa tacagcacct actataagcc ctggtatagc
    38881 aggaacttta aatacaacca atccaattac tattgctgga gctaataata atactggtga
    38941 taaagtatta aatgtagaat ctaatgtaga cctaattaat tctatttgtt ttatatctag
    39001 tgcttgacct gaatacttca ttccaattac accataaatt actaaacaaa ttaataatga
    39061 tggtatagtc gaatatagca tatattttat atgtgtaaac aaatctgttc ctgccattgc
    39121 tggagctaag ttagtcgttt cagataaagg tgatagttta tctccaaagt aagctccaga
    39181 tattatagaa cctacgatta atccaattgg catacctaaa ctttgaccaa tgcctaaaag
    39241 agctactcct actgtaccca ttgtgctcca agatgaacct gtcgcaagag atactattgc
    39301 acagactaat gttgatgcta ctaaaaaaat atttggtgat aaaattttta gtccccagta
    39361 aatcatacat ggaactactc cacttactat ccaagttcct ataagtacgc ctattataat
    39421 tatgattaag attgcttgca ttgccatctt tatactatta aacatactta attctaactc
    39481 tttccattta aatccaagtc tatatacagc cacaagtcct gctactgctg ctgctcctat
    39541 tagtggtaca tgaggtgatg aaccatacac taatactcct agacttaaaa ataatatcaa
    39601 acaagtaatt ggtatcattg catcaaacag acttgctttt ctacattctc gctctttcat
    39661 aaaatagttt cctcctaatt atttaaacac tacactataa ttttttttga aacatatttg
    39721 ctcgtactag atgacatgat acccccataa gttaataaaa attctaaatt gtcacctaat
    39781 ttataatcat attttgtgtt acttacatct agtatcatgt ggtctgaact tgcccctaat
    39841 atttttatat cagtgtctat tggaattaaa gagtcaatat ttatatcttg tttacctata
    39901 gctatgattg ccctttttaa gattccctta tcttcataaa caggtttatt tctaaaggca
    39961 tcaactccta tttctccaat aggaactgat ggtttttctt tacattcaac tacttggcat
    40021 attaatttaa atgcatcttg atatgttcca tttatatttt caccatatgc cgtttctcta
    40081 ccaagtagaa tggactcgcc tattctcaaa ttagtaatac cttctggcat attattattt
    40141 atcaacagat gaatagaact tgaattacct ccagatacta tttctagatt tattccaaat
    40201 ttttcttcca ttctttttgc tatacttacc agtctgccta tattttccct agatggtata
    40261 atagctccat aacaggttaa gtttgtagct ataccaatta tttcaatatt ttttaatctt
    40321 actatctctc ttacattact aaaaaagtct tcttcataaa agtatccttc tcgtaaatcg
    40381 cctaagtcaa acataagaac tatcttatga ataatatttt tactttcaca taattcattt
    40441 aatttcttta ttgttcctaa ttctgagtta aaactaatat cgcaataatt gataacttct
    40501 tctaattcac ttaacattgg gattctaatc aatatcttag gtagatttat atcttgtaaa
    40561 ttttttaagt tttgtattct tgaatctgct atatggtcta ttccttcact acatatttct
    40621 tctactattt ctttttgtgc acaaaatgat ttagtaacaa aagatatctt aatatttctt
    40681 tcatgacaca tctgcgaaat taattttgca ttatgcttta acttttctat atctatttct
    40741 aacctaggat acataatctt tcctcctcaa aattcaaact ttttaacagg agcttaagag
    40801 atgcttcttc atatttttta gataaaacac ccatagatgc cataatatag tcattatcta
    40861 ttaatatctt tgttttttcc tttggaaata gtaattcatt atttttattg tatttgagca
    40921 tattatctaa tatttcaatt ctactaggga gcctagtaag tgctccccct gtaccaacta
    40981 tatatcttac ttctgttaaa tctttccctt ctgcaatttt gacttttcca gttgttccat
    41041 agatatttct tatttttcct gaatgtctta gcatagcttt aacaacagct tcttttgtca
    41101 atctttcaac aaataatatt tcttcttttg attttggtat tggaggataa ttctctatta
    41161 ctgcatcaat atctatagat agttcacttt gtaagttttc tttacctata acttctacta
    41221 tattcttcat atttacatat acacctaaat ccccctcaac acttctcttt gcagtaggtt
    41281 caggattaac caaaatttta ttaatatagt cacttccatc tgtaacagaa tgtacatcag
    41341 tagttgctcc acctacatcc agagttaata aatctccaat atttttatat aaaagtttag
    41401 aagcttccat tacagctcct ggggttggag taatattttc attaaccatt tctttaatgt
    41461 atttcattcc tggtgctgta gttatgtgtt cttcaaatac actttgaatg attcttcttg
    41521 taggttctat attcaataaa tctattttag ggtatacatt ttcagttata taaagcttat
    41581 aattagtatc ttcaaatatt aaccttattt cctcttgatt ttcaacatta ccagcatata
    41641 taactggtat gcctagattc atagaagcta tcatttctgc attataaata gctgtatctc
    41701 tttctccata atcgactcca ccagctatta aaattatgtt tgggtttatt tcttttattt
    41761 tatttaaatc agttctttta attctacctg atgttatttg acgaataaca gctccagccc
    41821 ctaaagctgc ttctttagct gcttttactg tcatatcata gactaatcca tgaacagtca
    41881 tttttaatcc accagctgca ctagaagttg caaacatatc attatacttt atatcatcta
    41941 cagataattt attagctaaa tctttaatag cacctgataa gccatttctt acatcgccac
    42001 cttcaaacac tgttgttgga gcttgacctt gacccaaaaa tataggattg ttagtattaa
    42061 tatcgtggaa agcgtttacc actgtagtag tacttccaat ttcagctacc aacacatcta
    42121 tttttaaaga aaccttgcta ttttccatta tttattactc atttctcttc ttttttcaac
    42181 taagaaagtg gctacatgta tgcctttgct gtttcttcca aatccagcat ctatgccttg
    42241 ttcaacagct atttcaggtg ttacttgtgt tcctccacac gcaatcatta ctttatctct
    42301 tatacctttt tctacacaat aatcgtgtaa ttttttcatg tttttatagt gaatatcatc
    42361 atgacttatt attgtagatg caagtattgc atctgcattc agttcaatcg ctgcatctac
    42421 caatttttct acaggtacag aagtccctag atagtggcat tctatgccat atttttcaat
    42481 acctccatgt tttatatcta ttatttctct taaaccaact gaatgttcat cttctccaac
    42541 cgtagctgaa actattttca taggtttttc ttctatatct gctcttattt cttcatcagt
    42601 tagtacctta ggttcttctg gtattactaa attgtttaaa tctatatcaa acggaactct
    42661 tcctttcact tctattctag tcccttcaga ttcatgcata acttctctac ttatgacctc
    42721 aatttcttgt aagttcattt ttttgacaaa ttccaaagct gcaaactctg ctgttctttt
    42781 atcagtagga agcatcattg ttatcattac aattccatct gcacaccatt ccatttcagg
    42841 cttaatttta gttccatttc tgtactcata tgattctttt agtctatttt ctacattatc
    42901 aaaatcgtca agctcatcaa tatatattat tttgtctgga ttttcaaatg tacatccatc
    42961 tattaacatt gatggattat ctatagcatt ttcatcatat tgagctacat tattgtatcc
    43021 aaagtgagca gttacaggtg ctaaataatc ttcttctctt tcataaacag taccttcacc
    43081 aactccacct tttatttttc tagatattcc atcaccattt ctttctggat aatttccaga
    43141 atcaacaaaa aatccttgtt ccacagattc aaaatatcca ccaacttcag atatttcttc
    43201 catatataat acagctcttt ctttaagttc tcttgctttg tctcttaatt caccgacatc
    43261 tttaagttct atcatatcca taagcccatc cataccaact aaagcttgct ttgctgtatc
    43321 acatgcttct atattgtata tatgccaagg tacatttcta ccttcatcag gtgttatagt
    43381 tgattgtatg tcagctctag ttaatactga tgttaataag tttaatacat gagtaactgt
    43441 tgcttctcta gttgatgatt ccatatattt agtattcatt tgagctctca tcttataatc
    43501 actaaataac tctctaagtg caaccgcgta aggtaagtct atttttaaac atggtgctgg
    43561 aggtgctgtt ggtggaactg ttgataagca tatattactt ttatctatac ctattttttc
    43621 tgagaatata gagtttaaag cgtgttgaac cataagttca ggcattacct tccatgcttc
    43681 tctagcagta gcattagcat tatgtgctcc atcaatttga gctatattgg caaaagccat
    43741 tattttcttt gcctcacaag catctacaaa agaccttacc atgtttatat tcctatacag
    43801 aacattatat tgtgggtctt gatgtgctcc attgactcct tcctcagcaa acattactgc
    43861 tacttcagga ccagcaacac cacttacata tgaatggtaa ttaataggtc taccaacttc
    43921 ttcttctata aaatcaagag ccttcctttg agctcttact tgttttcttg ttattggaac
    43981 tccacctata ccttgtggag ttccttctat aagcccatca aaatgtgatt gtccagcagt
    44041 tctgataacc attagatggt cagctccatg atgagctgcc attctcattc ttcttatatc
    44101 atcctcaaat ctaccagaag ctatttcagt agtaataact ggacttggtt gagggtctat
    44161 attattgaaa tacttagatg atggtagccc tacactcttc tttaaaggtt ttgtacagtc
    44221 tttatattta aaaggtccca tttcaagatt ttccaagtgt tctctccaaa cccaacctct
    44281 tcgttttggt ttatatttat ccaaatcttt taatatatag tctatatcca attttttatt
    44341 ttcctttaac atgattctac cccttctcta aataaacatg taacttcatc ccaatattca
    44401 cccttaacta ataacaaacc tgcttctcta accgttatat ttttagattt agccaattta
    44461 taaactacat gccctgctcc tttacctata agacctcttt ccataacccc ttctaaaata
    44521 ggttttactt caagagaaga aaatcccatt ctcaacaaga cagacctttc tattgatggt
    44581 gttgtattct ttttaccaag ttctatcatt ggttctacta tctgggtagc taaactccaa
    44641 aacctttctt ttaactcatc atcactcagt tcttgaagat gttttcttct aacttcaaaa
    44701 tcatcctctc tttttttcat gaacattcct ccattcgtat tgatttaatc gaattttctg
    44761 aatttttatt ttatattaaa ttaaaaaaca ataattacta taaattattt aatacatcaa
    44821 caacaaaatc tcttgaactt tttacttctt ccattaaaaa ttctatatct cgattactta
    44881 gcttatcaat attatcaatg tgattattag atatacaatt ttttataaat gactttctta
    44941 ttttattcat atcaatatca acacatttca ataattctgg tctttctggt aatattaagt
    45001 ttactcctgc aatttcttct cttggatttc caaattttat atctattcca ttttctcttg
    45061 caaaggtaag ttgtggacat atatgtttac ctgcacccgt atattctgtt tcttgtacca
    45121 ctatagcttt atcctcatct agctcttgag ctaaagagaa tgctgctgct aaagatgtat
    45181 ttcctgctgg acctctttct ataccttcta gctgtgccaa taattcagtt gtataaaaaa
    45241 cttctccttg ttttactaat acatatctat ccatatatct aagtggtcta gctgcagacc
    45301 taggtacatc tgacctatca ggccatgtac agtatggtat cccaaaacct gtatgtcctg
    45361 ttgtaaatga ttttttatta aattgattat cactagccat gtgtaatcct tttaaatcta
    45421 cacttgctcc tactacttta gtattaatag aaccagcttt tatcaatcct cttgcagttc
    45481 cagtaagatt accaccacct gcatttgtac aaacaactat atcagggtct tttttatact
    45541 tttctctaaa ttggatagct aactcataac ctaaagtttc tacacctgca attccaaatg
    45601 gtgaatataa tgatgcgtta aaatagcctg tttcttctaa taaaactaag aacgtataaa
    45661 ataactctgg tccaacagtt aactgaacaa cttctgctcc atatgcctca cattttcgcg
    45721 ctttttctat gatttcaggt tgacctacac cattagaatc ataacattct tgaactatta
    45781 tacattttaa tccctgcatt gccgcttgtg atgctacagc tgcaccatag ttaccactag
    45841 tagctgctat tacaccttta tatcccattt gtttggcatg atatactgca gtagctgctc
    45901 ttcttgcttt aaaactacca gatgcattca ttgcttcatc ttttacaaat attctagctc
    45961 ctttaccttc tttagcacat tttctagcta atttagtcaa atttttaagc tctattaaag
    46021 gagtatttcc aacagcatac tgtgattgaa ttgcttcaat atcttgtaat gtatatcctg
    46081 tttcactcat cattttctca taatcaaacc ctattccctc ttgttcaaaa gaagaataat
    46141 ccaatccgat tgcatttttc attatttcgt tatttcttcc cataacagct tgataactca
    46201 tatctttcat tgatgttgaa ttattcatta tatacacctc caaataatat ttctttagct
    46261 tggattccta tttggagtaa ctctggtata catttaccat aatcatgagt ataatatgga
    46321 tttattttaa gtaaattacc ttttacaagt ctgcctgtaa ttgttttaac ttctactaag
    46381 tcgcctatag aagcatcact ttgtataaaa cctttaaccc acatttctaa ggatactttc
    46441 ttagtatcct ctggaacttg tggagctctt tcttctggag tcaatactat attgtgtata
    46501 ataacccaat cattaatttt agcatccatt ttttcatccc cttattaatt gactattgcc
    46561 ttactaatta atttactgtt attttaaatt aaacattatt ttaccaatta gctaactgtt
    46621 atcttactaa ttagctagtt gctatcttac taattagcct ttgattcttc ttccaattca
    46681 acttctatct gatttcttat atctcccatt attgctctag gtactggtat atccaacata
    46741 gttttaagac ctggtctagc atttattatt aatggaatag agtttactat cattgcataa
    46801 gtacctactc ctccaggtat ttcaggattt atttgtaaat caatgtttgg tatacctttt
    46861 attgatacat agtctcctgt tttaatacct tctgcttctg gtattatctg ttgtggatgc
    46921 tccatttcaa ttaaaacttc tccatttaca tatccatatc cacattgtct acaaccagca
    46981 acatttccag ccaatgcctc tccgtactta gattttctat ctactgtagt cattattggc
    47041 tcttttgttt gttctatctt atctaatttc caacctaatc catcagctat catatttatt
    47101 gattctacaa aacctacatg acctgctata gtcttatctt ctacaccttt tataaattct
    47161 tcccttgtta ctccaacacc ttgttccacc ataacagact ttccaaatgg agataaatca
    47221 tttactctct tagcttttat actagtaact tcttcacaag tacctgaaag tgccaatact
    47281 agtaaatcta atacaaatcc tggatttatt cctgttccaa gtatacttac tccattttcc
    47341 tttgcaactt catccatttt tttagctaaa tctgcatcat cagcttgtgg atatgccatt
    47401 tgttctgctg ttgatataac atttattttt ctatttaata agaaaattat tttatcaaaa
    47461 gcctttttag taaatgaatc agttgctaac aaaacaacat ctacactttt ttctctaaaa
    47521 acttcttcat aatttttatt gataataact tctggtcttt ctcctctttc aattcctaaa
    47581 acattataca tactttttcc tgaagtactt ctactacaaa cagatactat ttctatacct
    47641 tctttcttta gaatcatatt agccatacct attcccatag ctccaaatcc ccatattcct
    47701 actcttactt ttctcatata tacaccctcc taaaaattta tatattctca ataattagtt
    47761 aaagcataaa tcgtgccaac tttaaaatta aagtaaatat ttattggaac gttgaaacta
    47821 taattatttt attatgtatt ttcataaata aaaaataata tttcttatga gaaataaaaa
    47881 aaagagcaaa atattttgct cttttataat aattttgctc ctctattttg cttttttatt
    47941 ttactttgat tttgtttttt tatcttgctt atttataata ttttgatatt atattttttt
    48001 attttatact gtaaattttg cctattcata cctaaaattg atgcagcttt agttatattg
    48061 taagatgcct tttctaatga ttcatatata tacttttctt ctatctcact gataatctta
    48121 ctaagtgttt tgtcctccaa atcgtttaaa gaataattgt tactctcttt attctcttta
    48181 agattgtaat agtcgtttag attaatcata cttgtttcta tttgtacctc atcttccatc
    48241 atactcatag tacaataaat tatgttttct agctctctta tgttaccttg ccaatcgtaa
    48301 ttttttaaaa ttttaatagc ttcatctgaa attcctacca cttttttatt taacttttta
    48361 ttatactttg aaataaattt tttgcacaat agaggtatgt cctcttttct atctctaaga
    48421 ggaggaattt ttatatgaat tatgttaagc ctataatata aatcttttcg cattttatgc
    48481 tcgtttaaaa ttacctttgg actttcattt gtcgttgcaa taattcttac atctataggt
    48541 atatccttag ttcctccaac ccttcttaca taaccttctt gtaaaaccct taatagttta
    48601 gcctgtaaca taataggcat ggaatttatc tcatccaata acaatgtccc tttgttagct
    48661 tgttcaaata agcctggcct atctattgct cctgtaaatc ctcccttgct agttccaaaa
    48721 agaatacctt cgaatagtga ttccggcaaa gctgcacaat tttgtgctat aaatggatag
    48781 tttttcctat tacttccata gtgaatggac tggctaaata actctttacc cgttccagtt
    48841 tcaccgtata taaatactga agcatcagac ttgcaagctt tcttagattt agctattgct
    48901 tctttaatca aattactttc ccctaaaata tcatcaaatt cataaccctt tttttgtcta
    48961 ctactaactt tattgactaa atttttttta taatttctat ttagtaaatt ttctatcgtt
    49021 gtcatattct ttgatatctc aagtgcccct acaaactcgc ctttaatatc aattggtatt
    49081 gtagtattaa ttgctgttat ctcttttcca tttttatcca gatatttttg aatattgtct
    49141 ttgataactt cccttttttc caaaacttct aatagtgtac tcctgtttat tttcattttg
    49201 ttgactatat caaaaaatga attggttaaa atatcgtgtc tgttcatttt ttccattttt
    49261 tgtacagaat tattgtagat tatagttttc ccgtctttat taacaaaatg taccccttca
    49321 tcaatatttt ccaatatttc attcattatt ttaaaaaata tctcttggtt caactatatc
    49381 accccagtac ctaaatatat aaaaacccaa aaaattaata aaatgttgct attgtattta
    49441 tcttatcata tatttatgat attttttatg tacattatat taatatattt ttaagtatat
    49501 ttaaaatttt atttatatat ctttcattat ggttatatac aatatttgaa ttgtcatta
//
LOCUS       DAELNY010000008        49327 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.008, whole genome shotgun sequence.
ACCESSION   DAELNY010000008 DAELNY010000000
VERSION     DAELNY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.008"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            90..1691
                     /locus_tag="KPE55_000405"
     CDS             90..1691
                     /locus_tag="KPE55_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG0491507.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1910..3508
                     /locus_tag="KPE55_000406"
     CDS             1910..3508
                     /locus_tag="KPE55_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0491508.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            3798..5996
                     /gene="slpA"
                     /locus_tag="KPE55_000407"
     CDS             3798..5996
                     /gene="slpA"
                     /locus_tag="KPE55_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG0491509.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASLSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            6242..8584
                     /gene="secA"
                     /locus_tag="KPE55_000408"
     CDS             6242..8584
                     /gene="secA"
                     /locus_tag="KPE55_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0491510.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            8998..10869
                     /locus_tag="KPE55_000409"
     CDS             8998..10869
                     /locus_tag="KPE55_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0491511.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            10978..11685
                     /locus_tag="KPE55_000410"
     CDS             10978..11685
                     /locus_tag="KPE55_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0491512.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            11718..13850
                     /locus_tag="KPE55_000411"
     CDS             11718..13850
                     /locus_tag="KPE55_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0491513.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(13903..14298)
                     /locus_tag="KPE55_000412"
     CDS             complement(13903..14298)
                     /locus_tag="KPE55_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG0491514.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            14525..16936
                     /locus_tag="KPE55_000413"
     CDS             14525..16936
                     /locus_tag="KPE55_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG0491515.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            17233..18810
                     /locus_tag="KPE55_000414"
     CDS             17233..18810
                     /locus_tag="KPE55_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG0491516.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            18944..20281
                     /locus_tag="KPE55_000415"
     CDS             18944..20281
                     /locus_tag="KPE55_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG0491517.1"
                     /translation="MGIGIECNKKINKSIDKTRIKKNNLNSNSNSNSNSNSNSNSNSN
                     SNSNSNSNSNSNSNSNSNSNKISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGT
                     WMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKR
                     SILLILFGFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIIT
                     FLYIATFIIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAIS
                     SGMLGCTMGCVLLKENLGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLL
                     MAGSYGILLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNK
                     VDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            20535..22559
                     /locus_tag="KPE55_000416"
     CDS             20535..22559
                     /locus_tag="KPE55_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0491518.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            22779..23465
                     /locus_tag="KPE55_000417"
     CDS             22779..23465
                     /locus_tag="KPE55_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG0491519.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            23560..24615
                     /locus_tag="KPE55_000418"
     CDS             23560..24615
                     /locus_tag="KPE55_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG0491520.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            24730..26286
                     /gene="murJ"
                     /locus_tag="KPE55_000419"
     CDS             24730..26286
                     /gene="murJ"
                     /locus_tag="KPE55_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0491521.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            26332..28038
                     /locus_tag="KPE55_000420"
     CDS             26332..28038
                     /locus_tag="KPE55_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0491522.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            28060..29133
                     /locus_tag="KPE55_000421"
     CDS             28060..29133
                     /locus_tag="KPE55_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG0491523.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            29145..29867
                     /locus_tag="KPE55_000422"
     CDS             29145..29867
                     /locus_tag="KPE55_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0491524.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            29883..31040
                     /locus_tag="KPE55_000423"
     CDS             29883..31040
                     /locus_tag="KPE55_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG0491525.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            31043..31882
                     /locus_tag="KPE55_000424"
     CDS             31043..31882
                     /locus_tag="KPE55_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0491526.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            31897..33072
                     /locus_tag="KPE55_000425"
     CDS             31897..33072
                     /locus_tag="KPE55_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG0491527.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            33101..33850
                     /locus_tag="KPE55_000426"
     CDS             33101..33850
                     /locus_tag="KPE55_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0491528.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            33872..34780
                     /locus_tag="KPE55_000427"
     CDS             33872..34780
                     /locus_tag="KPE55_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0491529.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            34828..35580
                     /locus_tag="KPE55_000428"
     CDS             34828..35580
                     /locus_tag="KPE55_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0491530.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            35534..36901
                     /locus_tag="KPE55_000429"
     CDS             35534..36901
                     /locus_tag="KPE55_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG0491531.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            36907..37998
                     /locus_tag="KPE55_000430"
     CDS             36907..37998
                     /locus_tag="KPE55_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0491532.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            38082..39995
                     /locus_tag="KPE55_000431"
     CDS             38082..39995
                     /locus_tag="KPE55_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0491533.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            40194..40901
                     /locus_tag="KPE55_000432"
     CDS             40194..40901
                     /locus_tag="KPE55_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0491534.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            41203..43314
                     /locus_tag="KPE55_000433"
     CDS             41203..43314
                     /locus_tag="KPE55_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG0491535.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(43512..44477)
                     /locus_tag="KPE55_000434"
     CDS             complement(43512..44477)
                     /locus_tag="KPE55_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0491536.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(44762..45718)
                     /locus_tag="KPE55_000435"
     CDS             complement(44762..45718)
                     /locus_tag="KPE55_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0491537.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            46086..46874
                     /locus_tag="KPE55_000436"
     CDS             46086..46874
                     /locus_tag="KPE55_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0491538.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(46997..47467)
                     /locus_tag="KPE55_000437"
     CDS             complement(46997..47467)
                     /locus_tag="KPE55_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG0491539.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            47606..48250
                     /gene="uppS"
                     /locus_tag="KPE55_000438"
     CDS             47606..48250
                     /gene="uppS"
                     /locus_tag="KPE55_000438"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG0491540.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            48293..49198
                     /locus_tag="KPE55_000439"
     CDS             48293..49198
                     /locus_tag="KPE55_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0491541.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        19771 a         5436 c         8968 g        15152 t
ORIGIN      
        1 atatactcta taatatatgg aaaataaaga gaaaataaat ggaaaataat cacaatataa
       61 atataaaata taaaaatcat cagggggata tgaaaatgaa caaaaaaata ttatcattag
      121 gtctagcagt atcattaatc ttagtaaact tcaaaagtgt aaatgcatca tcagtagtag
      181 aaaaaatata cggtaaagat agatatgaaa cagcagcaaa gatagctgat aaacagactt
      241 atgaaacagt aattttagta aatacagaaa aatcacttgc agatggatta agtgcaagtg
      301 gattatcggg agctacaaaa gctccaatac tatttacaca acaaaataag ataccagcag
      361 acacaaatag atgtctaaaa aatatcaaaa aagcatatat aataggtaca gaagatacta
      421 taagtaaatc agtagaaaaa gaactagatt ctaaaaatat agaagtcaaa agaattggtg
      481 gagaagatag actaaaaaca agctatttaa tagctaaaga aatagcaact ataaaaaaag
      541 tggataaagt actattaact aatgcatata gtggagaagc agatgcaatg agtgtatcat
      601 cagtagctac tagagatgga gctccaatca tacttacaga tggaaagagt gtgccttttg
      661 atgtaaaaaa tattcaatca tattgtatag gttcagagga gataatgagt aatcctctag
      721 ttaaaaatac aaactcagta agaatagaag gaactgaccg atttgaaact aacaaaaacg
      781 taatagacta cttttttaat agtgcagatg gattctatgt atcagatgga taccaattag
      841 tagatgcaat agcagctgca ccacttacta aaaactctcc aatggtacta gtaaatgatg
      901 gaagcgataa aactgtatta gaaggagcta agaatataac ctctgtaggt gaaataaatg
      961 agaaggtaat acaacagtgt ataaatgctt caaattcaaa tgggcaaccc ccaacaatta
     1021 cagttggaag tacagaggta tataagggtg aaaagtttga cactggcaag ttaaatatag
     1081 tagctaaaga taacacaggg aaggtattac caatagaagt tgatgggttt atagatacaa
     1141 atagagtagg tacatacata ttgacattaa aagctacaga cgaatgggga aaaagcacag
     1201 gaaaaagagt agaaataaaa gtgttggatg acaaatctca tgattacaac agcccagaat
     1261 ttaaaaaaat ggtatctact gaaatgtata acttaatcaa ttcctataga aaagaaaaag
     1321 gcaaagagcc tttgctagtg tctagtaggc tagaaggtat ggcaaatgca tggtctaagt
     1381 atatgatgga taaaaaagta tttgcacatt atatagatgg aaaaaatgct ccacaagtat
     1441 tttctgaatt tggaatgaga agtgaggaaa atatagcata tatttatata gattctaaga
     1501 acgttcaaac gacacaagat gcaaaagact tggcaaaggc tatatttgag gtgtggaaga
     1561 agtctccaga gtacaatgca aacatgctta gtaatgagtt ttatagtact ggatttgggc
     1621 tttatatatt atctgatggt caagtgcatg ctactcagga gtttttaaat ggaaatgaag
     1681 gtagcttgta aaaaattcca taaatcttgt ttttttgtta atttactgat attataaaat
     1741 gaaaatcaca tctaaagtac tttttgtaat ctgttttgtt attgctttac aggctatggt
     1801 attttaggtg ttttttgttt tagtagttta tagattttga gggaatcact actgaggatg
     1861 taatttatga ttagctgaat tatataaaaa aattagtagg aggagtttga tgaaggtaaa
     1921 taagagagta ttatcaatag gattgacaat atcattaata atggcaggag caccaaatat
     1981 caatgcactt tcgagcatag aaaagataca ggggaaagat agatatgaga cttctgcatt
     2041 gatagcagat aaacaaatat atgatacaat catattagta aatactgata attctatagt
     2101 agatggatta agtgcaagtg gattatctgg agttgcaaaa gctcctataa tgttggtgca
     2161 aagagataag ataccaacag atgttgaaaa aagactaaag gatgttaaga atgcttatgt
     2221 tataggtaca gaggatacaa taggtaaatc tgtagaaaat caacttaaga ataaagggat
     2281 tgaagttaaa agaattggtg gggaagatag aataaagact agctatctta tagctaagga
     2341 gatttcagct attaaaccag taaatgatgg tgataaggtt tttcttgtaa atggatacac
     2401 aggtgaggct gatgcaatga gtgtatcttc tgtagctgct agagatggag ttcctgtgat
     2461 acttactgat ggaaagagta taccatttaa agttgatggc gttcagtgct attctcttgg
     2521 gtcagaggag ataatgagta atgaacttgt gagtaagact aattcagtta ggattgctgg
     2581 taaggatagg tttgaaacta ataagaaggt tatacaacgc ttttataaag gaacaaagaa
     2641 gttttatgtt tctcaagggt ataagttagt tgatgctgtg gctggttctc cgttggctaa
     2701 ggacaaacct atagtacttg ttaatgatgg aagtgataag agtgtgctta gaggtgctga
     2761 tgaagttact tctttaggtg gaatggataa gaaggttgtt gaccaatgta ttagttctgc
     2821 atctgataaa aatacaatgc ctacaattac tgctaatgat gtagagattt ctgtagggga
     2881 taagtttgat aatagtatgt tgaatatagt tgctactgat tattatggaa atgatttaaa
     2941 ggcaaatatt aaaggaaatg ttgatattaa caaggctgga acatatgttt taaatataag
     3001 tgtagtggat aatttaggtc agaagagtga gattagtgta aatgttaagg tagttgtaaa
     3061 tgcgagtaca aaagactcta atagttatga gtttaaggct atggtatcta atgagatgta
     3121 tgatttagtt aattcatata gaaaagaaaa aggcaagaag agtttaagag agttagactc
     3181 tttagctgga atggcaaatg catggtctaa atatatggaa gataagaagg tatttgccca
     3241 tgagattgat ggtaaaaatg cagctgaagt attttttggc tttggagcaa gaagtggtga
     3301 aaatattgca tatctaccaa tgaatgttaa atctgtttat acttctaaag atgcaaaaga
     3361 aatggctgag tctatttttg atttatggaa gaagtcttct aagtataatg aaaatatgtt
     3421 aaaagaagaa ttttatagct ttggatttgg tatgcatgtt tctagtaaag gagaagtgaa
     3481 tgcgacaatg gaatttttaa atagttagat tttatattaa tttacattgg aaaagatata
     3541 gtaaatttgt aaaaataaat atataaaatt aaaataactt ataaaattat aaaaaataat
     3601 ttaaaaataa taaaaatgat gaaaaaacaa ttaatttgtt ggaaaaaact tcaattaatt
     3661 ttgcataaat tattgaattt atattttata tagtctatca taagagtgtg agaaatattt
     3721 ggattatatg aacatggaaa tttaatgtac ataattaaag agatgtaatt aatataatgt
     3781 tgggaggaat ttaagaaatg aataagaaaa atttagcaat ggcaatggca gctgttactg
     3841 ttgtaggttc tgctgctccg atatttgctg atactactgt taaagaagaa ggatatacgg
     3901 tagtacaaga caaatatgaa aaattactta aagaattaaa ggcaaagata aaagatggaa
     3961 ctattacatc agttggtgtt gaatttgatg gaaaaccaat tactacatta gcaccaaagg
     4021 cagatggtag tgataaagat gcaatagcag aacagttaga aactcttact aaaaaccaac
     4081 ttaaaggttt aggtgatgga aaatatgtag attttaaaat tacttatggt gctaaagcag
     4141 aagttccagc tgcttcatta agtgcagatg atatacaaaa atatgcagat caaataaatg
     4201 cttctgagaa aatattagtt gaagtagcag caggatctga agcaggtata gcaaaatttg
     4261 attctgttaa caataaagtt atagcaggag atgctcctct taaagttaaa gatgcagtga
     4321 aagcgactgt tactactaat ggtagtaata aaaaagttct tacaatatca gctgcagctg
     4381 gattatcagg attttcatat ggaacactta aagacacagg tgcaagttta tctgatgttg
     4441 atgcaataac tcttgacact acaaatgcaa caataacaga aggtgatact aaagtattag
     4501 attttgataa ttcatttaag tttaacgaaa gtactaaaaa agttggtagt ttagttacac
     4561 caaatacaac aaacactcca gcagatcctg gtactaaaac tactgttaga gtgataaaag
     4621 ctgttgaaaa aacaatagat gtaagttcaa attcaactac aaaagctaaa gatttagcta
     4681 aacaatatgt attcacagat gttagtgata ctgatccaga aagtttaagt tatatgctta
     4741 aaaacataaa tgatggaaaa gttgctgtta aaaattctga tggagattat gaagtaacta
     4801 tattcccaga aggaaaaaga ttaaatactt tatctgcttc ttcagctaaa actattttag
     4861 gagataaaga tactccagca aaaatagttt taaaggcttc aacaacaaag aaattagcag
     4921 actatataga tgatttaata acttataaca atagttattc aaatgtacaa actgtagcag
     4981 gtagtgatag aatagagact gctatagagc taagccgtaa gtactataac tctactgaca
     5041 aaaatgcttt atatggagat cctgtaaata acgttgtatt agtaggttct caagctatag
     5101 ttgatggatt agtagcatct cctttagcag cagaaaaaga tgctccttta ttgttaagtt
     5161 ctaaagataa attagattca tcaacaagag ctgagataaa aagagttatg gacttaaatt
     5221 cttcaacagg aataaaaaat aataaagaag ttttcatagc aggtggagta aattctatat
     5281 ctaaagacgt tgaaaatgaa ctaaaagata tgggacttaa agtaactaga ttatcaggtg
     5341 atgatagata tgaaacttca ttagcgatag ctgatgaaat agatataaat gataaagctt
     5401 atgtggtagg tggaacagga ttagcagatg ctatgagtat tgctccagtt gcatctcaaa
     5461 taaaagatgg tgaagctact ccaatagtag ttgtagatgg aaaatctgac aagttatcta
     5521 aagaagctga agatttctta gatgatgcac aagtagatat aatcggtgga gaaaatagtg
     5581 tttctgctaa aatggaagat tatatagatg atgctactgg aaaatctcca gaaagaatta
     5641 gtggtgctga tagacaagca actaatgctg aagttataaa agaatacttt gataaagatg
     5701 gagtttcaaa ttacttctta gcaaaagatg gttcaactaa agaggatcag ttagttgatg
     5761 cattagcagc agcagcagta gctggaaact acggttctaa gcataatgaa gatggagata
     5821 taactacaga tgcttctcct gctccaatta ttttagcaac tgataattta tcagctgaac
     5881 aacatgtagc tgtaagtaaa actgctacta ctaatggtgc taagaactta gtacaagtag
     5941 gtcaaggaat agctgattca gttgttagca aattaaaaga tttattagat atgtaatata
     6001 aattttatat attgaataaa aaaggtttct cttttgagaa acctttttta ttttaaacat
     6061 aaatttttat aaataaaaag aggctaatta gcctcttttt aatgtgagaa acacttaaaa
     6121 aataagatgt aattatttat ttaagtatca ttattatact ataccttaaa ataaatgtct
     6181 actattctat aattttaagg tataatagta agtggaaaat aaataaggag gtaaattaaa
     6241 tatgtcagtt ttagatgtaa tattagacaa aacagatgaa ttagaaatta aaaaattaaa
     6301 taatatagta gataaaatag atgctttaga agataaaata caaatactag atgatgaagc
     6361 attaaaaaat atgacgaatg tatttaagga taagttaagt aaaggagaaa ccttagatga
     6421 catattgcca gaagcatttg ctgttgtaag agaagtatca aaaagaatat taggtatgcg
     6481 tcaatataga gttcaattaa ttggtgggat tgtattacat caagggaaaa tagcagagat
     6541 gagaactgga gaaggaaaga ctctagtagg agtagctcca gtttacttga atgcattgac
     6601 tggtaagggg gtacatgtca tcacggttaa tgattacttg gctcaacgtg ataaagaact
     6661 tatgcaacca gtatatgaat ttttaggttt aacagtagga gtaattatta atgggcaaca
     6721 gccagtagaa cgaaaattac aatatcaatg tgatattact tatggtacaa acaatgagta
     6781 tggattcgat tatttaagag ataatatggt aacgcaaaaa tcagagaaag ttcaaagaga
     6841 actaaacttt gctatagttg atgaggttga ttcaatactt atagatgaag ctagaacacc
     6901 acttataatt gctggtggag gagatgaagg tactaagctg tatgaacttg caaatgcttt
     6961 tataaagaat ataagagatg aagattttga aatggataga aaagataaaa ccatagcact
     7021 tactgcaagt ggtcttagta aagctgagaa attttttgga atagaaaata taactcatat
     7081 agataatata gaaatttttc accatgtaaa tcaatctcta agagcacata aattaatgaa
     7141 catagatgtt gaatatgttg ttagagataa tgagatacta attgtagatg aatttacagg
     7201 aagaataatg gatggaagaa ggtttactga tgggcttcat caagcaattg aagctaaaga
     7261 gggagtaaaa ataaatgatg aatcaaagac catggcaaca gtaacatttc aaaatttctt
     7321 tagattatac tctaaattat caggaatgac aggtacagca aagacagagg aaagtgagtt
     7381 tgaagctaca tataagatta atgttgttca gataccaact aataaaccag ttataagagc
     7441 tgatttacat gataaagtat tcaaaacaga acgtgcaaag tacaatgctg ttgtagaaga
     7501 aataataagg ataaataaaa ctaagcaacc agtgcttgta ggaactgttt caattgaaaa
     7561 atctgagaaa ttatctaagc tacttaaaaa gaaaggtgta aagcacgagg tcttaaatgc
     7621 taaacaacat gataaggaag ctgaaataat atctaaagct ggtaaattag gttctgttac
     7681 aattgctact aatatggcag gtagaggaac tgatatatca cttggagctg gagataaaga
     7741 agaagaagaa aaggtgaaag agttaggtgg cttgtatgtt ataggtactg aaagacatga
     7801 gacaagaaga attgataatc agcttagagg gcgttcggga agacaaggtg accctggaac
     7861 ttctagattc tttgtaagtt tagaggatga agtaataaaa ttgtatggtg gaaaaagtat
     7921 agaaaaaata ttaaagagaa tgaatgctaa tgaagatgac gcgttagaaa gtaaggcttt
     7981 gaatagagca atagaaagag cacagaaagg cattgaaggt aaaaattttg aacaaagaaa
     8041 gaatgttctt aagtacgatg atactattaa tgagcaaaga aaagtaatat atgatgaaag
     8101 aaataaggta ttggatggtt ctgatattga agaagagata caaaaaatgg ttatggatat
     8161 tattacggag gctgctgaaa agtacttaaa aaaatttaga gactatcatg gatattttaa
     8221 gtatttatat aatactttta tgccagctga tactttatta attccagact tagataaaaa
     8281 aaatgttgaa gaaatagtaa atcaaacata tcagatttca aagcgtgttt atgatcttaa
     8341 gaagatgatg attggaattg aagaattggc tcaattagag aaacgagtat tacttcaagt
     8401 tgtggatact tattgggttg accatataga tgcaatggat caactaagac aatatatagg
     8461 tcttaaatct tatgcacaaa aagatccatt taaagagtat gctatagaag gttatgaaat
     8521 gtttgaagct ttaaatagga atataagaat aacaacagtg caatatctat ataaatttaa
     8581 ctaaaaaagt gacccattgg gtcacttttt atatattaag attaaagtga aatttatttt
     8641 acagtacaca ctcatataga taaatattta taataatgaa aaagaggctg aacgtcagcc
     8701 tcttttcctg tgtgagaaat acggaaaaaa gagatgcttg aaatttcttt cttccattta
     8761 atattatact ataaagagaa taaaaagtcc atgagttatc tttatgatta aaaaaaaaaa
     8821 ttacaatttt gttacttttt agtatacaat aataaattta aattgacttt gttaattttt
     8881 attaataaaa tatccaaata ttacaatatc tacagtttat tgaaataatg tgtaaattat
     8941 attataattg gtaaataagg aaaaatggtg aaaaccaaat tttgtagggg gaaatatatg
     9001 aatagaaaaa atctttatgt aattatggca gctgcaatgg taagtacatc agtagctcca
     9061 agttttgctg cagaagctac acaagtaaag aaacaaacaa taactaaaaa ggaagcaaca
     9121 gaattagttt ctaaagttag agaactaatg gctcagagat atacaggagg ttctcaagtt
     9181 ggacaaccaa tatatgaaat aaaagttggt gaaacttcat cacaattaaa aataataact
     9241 aatatagacg aattagagaa attagtaaat gctttaggag aaaataaaga gcttcttgtg
     9301 actatagcag ataaaggaca tacaacaaat agtgcaaatg aagtggttgc tgaagcaatt
     9361 gaaaaatatg aaaattcagc agacctttca gctgaagcta attcaataac agaaaaagct
     9421 aaaactgaag ctactgggat ttatggagtg gcaactgtag aagcttcata tgacagtaca
     9481 aaagataagt tagtaataac tttaaaagat aagactgatg tagtaacttc taaaacgcta
     9541 caaataggta ttggtgatga aaaggttgat ttaacagcaa atccagttga tatagcagga
     9601 accaatttag acccttctgc agaaggattt agagtaagta aaataaataa attaggtgta
     9661 gaaggagcta aaaatattgc tgatattcaa ttagctgaaa taactataaa aaatagtgat
     9721 ttaaatacag tttcacctca agatttatat gatggatata gattgactat tcgaggtaat
     9781 acaatagcaa atagtgtagg aaaatcaatt agtgatattt catcaaaaga ttcagagaca
     9841 ggaaagtata aatttactgt taaatatact gatacatctg gaaaagcgat agaacttact
     9901 gtagaaagta ctaatgataa agagttaaaa gatgttaaag atgccttaaa tggaaataca
     9961 aaagtcaatt taatagctgg ggaagacagg tatgcaactg ctgtagctat agcaaaagaa
    10021 acagaatata ctgacaatat agttatagtt aattcaaata aattagttga tggattagca
    10081 gcaacaccac tagctcaatc taaaaaagct cctatattgt tggcgtctga taatgaaata
    10141 ccaaaagtaa ctttagatta tataaaggaa ataattaaaa aaagtccatc agctaaaata
    10201 tatatagtag gtggagaatc agcagtatca aatacagcta gaaagcaatt agaatcagta
    10261 actaagaatg ttgaaagatt ggctggagat gatagacata caacttcagt agcagtagca
    10321 aaagctatgg gttcttttaa agaagcattt gtagtaggtg caaaagggga agctgatgct
    10381 atgagtatag ctgctaaagc tgctgaactt aaggctccta taatagtaaa tggttggaat
    10441 gatcttacag cagatgctat caagttgatg gatggaaaag agattggtat agttggtggt
    10501 tctaataatg tacctagcca aattgaaaat caacttgctg atattgataa agatagaaag
    10561 gttcaaagag ttgaaggtga aacaagacac gatactaatg ctaaagttat agaaacatat
    10621 tatggcaaat tagataaact atatatagca aaagatggat atggaaataa tggtatgtta
    10681 gtagatgcat tggcagcagg tcctctagca gcaggaaaag gaccaattct tttagctaaa
    10741 aatgatataa caaactcaca aaagaatact ttagataaaa aactaaatct tggtgcagaa
    10801 gtaactcaaa taggtaatgg ggttgaattg actgtagttc aaaaaatagg caaaatacta
    10861 ggatggtaat aatcaataat atagaaaagg attccttatt atttatagga atctttttct
    10921 atcgacataa tattcattaa agtataacat gctcttgaca gataggagga tatgatagtg
    10981 atagaagcaa cattgttttt ttccccacac caagatgatg aaacattaag tatgggtagt
    11041 gcaataattg aacatgtaga aagaagcgat acacatgtta tattatgtac tgatggcagt
    11101 aaatcaataa taagaaaagt attagatgat ggagggtcat gttcttatca tattaaagat
    11161 gtacacaagt actctttatc agaaagtgat ttttcaaaaa atagagatga agaatttaaa
    11221 aatagttgta aagcaatggg agttaaagaa aataatatac atatagaaaa taatagagct
    11281 catgatggag aattgagtaa ggaaaaagca agagatataa tgctaaaata tttagaaaaa
    11341 tatcctgatg ctaaagtgaa gaccgttaca ccatataaag ctcttggaat acatgaagac
    11401 catagagcat taggtgaagc tgcattagaa ttgtatagag aaggaaaaat aaaagattta
    11461 agattttatg tagagccata cgattataat gattttaaaa aagttaatcc aaatgttaga
    11521 gtatggaagg ttttgagtag tcaagaagag aaattattaa atgctatgga tgcatacaag
    11581 aaatggaatc ctgaaaatgg taactacgca attggatatc attctgtaaa atctcacttt
    11641 gatgaattaa aatccaataa aatccaatat gtacatgctc cataaacatt aatataaaaa
    11701 ctaggaggat atagttaatg aaaatatcta agagaataat gtcgttgttg acagcaactt
    11761 tttttatctt tgcattatct atcaatatag ctgatgcttc atcaaaagag agtctagtgg
    11821 gctctggtag atgggaaact gcaataaaaa ttagtcagaa tggatggaaa gatgctacta
    11881 atgctatact agtaaatgat aattctatag ctgatgcatt atctgcaact ccatttgcta
    11941 agttaaaaga tgcacctatt ttattaactc aaagtgataa attggatagt agaactaaag
    12001 cagaattaaa aagactaggt gtaaaaaatg tatatttaat tggaggtatg aatactctaa
    12061 gcaccaatat tgaaaaacaa ttaaaatcag aaaatatgac ttttgagagg atttcaggta
    12121 atagtagata tgaaacatct ttaaaattag ctgaaagatt agataaagag aagagtattt
    12181 ctgatatagt tgtagttaat ggagagaaag ggctagcaga tgcagtaagt gtgggttctg
    12241 ctgcagcaca agaaaatatg ccaattatac tttcagatcc taacaatgga ataaaagttg
    12301 caaatagctt tattagtagt aaaaatataa agaaatctta tataattggt ggtacatatt
    12361 ctgtatctag ttctattgaa cagagtttac ctaatgtaaa aagattatct ggaaacaata
    12421 gaaatgaaac taatgcaaag gttctagaag aattttataa aaatacagac ttaaaaaatg
    12481 cttatataac taaagatggt atgaaaaaac agggggattt gatagattct ctagctgtag
    12541 gtgtattggc agctaaaaat agctctccag ttgtattagt aggaaattca cttgactcta
    12601 gccaatatga tgtgttaaat actaaaagtt ttgataatat tgtgcaagtt ggtggaaatg
    12661 gaaatgaaga tgctgtaaag gatattcttg atttacaaga agaaactata tatagagtta
    12721 agacagcaga cgaattggat gttgctctaa gaaaagcaga tgctaatgat attataacat
    12781 ttaaacctga tgataataaa aaaattacta gttcatttag tatagaaaca aagaaagcta
    12841 taactgtaga gcttgaagga ttgtatacac aaacaataac tgtaaatatg ccaaatggag
    12901 aattaactaa taagggtaca ttagatggaa ctgtgaaaat aatggatata aaaaatggca
    12961 catttatcaa tgaaggtgat ataaatgata ttgacattta tgatccaaat ggatgtaaaa
    13021 tagagaataa aagtggtgga gatatatggg ttttaactat atctagtagt gctaaagatg
    13081 taaatataga aaataaaggt gatataagta aggttacaaa taattctact agcgttataa
    13141 taagaaattc aggaaatatt gatactttaa gtggaattaa tgcaccagca ataagtggaa
    13201 ataaacctag agttgatgat accgataagg atgaaaaaag agcaagtgga atatatccta
    13261 cttcagaacc tttatcacct gcaagagctg gttatataag tgtaaaaata ttcactaagc
    13321 ctgaagatac aaattataaa atttattata gagtagtaag cagtagacca tctgcattat
    13381 atatcggaga tagaatagat acaagtgact ggactgctgt accatcttct gttttaccat
    13441 ttactattaa agcagaaaat ggatattata tagaagctgt tgaaataaat tctgatacta
    13501 agaaagtatc tagatggggg aaatctgact atatagatga tggattttat ttaaaatcag
    13561 aaactgcaaa aggattaagt gcagatatag attttgatgg agattatgtt aaattaacag
    13621 caacaggtgc tgattctgga tgtacagttt attatagaat agtatcaaaa aagccagtat
    13681 caatgaatgt tggagataaa gtaactatat ctgattggga tgaaattact actaatagag
    13741 tgaggataga agcaaacgat gtaaaggata aatatataga actagttgaa ttggatgatt
    13801 caactaataa agtaactaga tggggagcaa gtgataaggt agtagaatag gtatctaatg
    13861 tgaaacataa aaacagactg attaataatg ggcagtctgt ttttatttcc taaaaattat
    13921 cattttacta aataaataat ttaaaactat aacaattata ttagtaaata cttttacagt
    13981 aatatcattt acaaaaaata aatcaatcat tatatacata ataatcattt ctataatacc
    14041 agaaagtagc ctaaaactga caaaaaacaa aaactcctta aacaaaaata ttagttcaat
    14101 tcttttactc tcaaacacaa aaaacttatt agtaacataa gcaaataaaa cagcaagtat
    14161 ccatgccaaa gcatttgcaa ccataaaatt aaataaaata actctagtaa aaaataaata
    14221 cgaaactata tttacaagag tagtaaatgc tccaaaaaat aaatataata tcgtctctct
    14281 atgctttttt aaaatcaaat ataatcctcc taagctaatt taatataaat atacttaaag
    14341 atactaacaa acattagtat aacacatcta ttaataaatc aatatatctt aaaatattat
    14401 aaaaataaaa ctataatatt ctataattaa tatgtactca taatccatat tttatcaaaa
    14461 taaaatatga ccttaattta gaaatctgat ataataaaaa ttaaatacat ataagggggt
    14521 aaacatgaga aagtacaaat caaaaaactt gtcaaagcta ctagcattgt tgacagtttg
    14581 ctttttaata gtgtcaacaa tacctgtctc agcagaaaac cataaaactc tagatggagt
    14641 agaaactgca gagtattctg aaagctatct tcaataccta gaagatgtca aaaatggaga
    14701 cacagcaaaa tataatggag taataccttt tccacatgaa atggaaggta caacacttcg
    14761 aaataagggt agaagtagtc ttccatcagc atataaatca agtgtagcat acaacccaat
    14821 gaatcttggt cttactacac cagcaaaaaa tcaaggaagt cttaatacat gctggtcttt
    14881 ttcaggtatg tcaacgttag aagcatatct taaattaaaa ggatatggaa cgtatgactt
    14941 atcagaagaa catttaagat ggtgggcaac tggtggaaaa tatggatgga acttagatga
    15001 tatgtcaggg agttcaaatg taacagctat aggatatctg acagcatggg caggtcctaa
    15061 gttagaaaag gacataccat ataatcttaa atctgaggca caaggtgcaa ctaaaccttc
    15121 aaatatggat actgcaccta ctcaatttaa tgtaacagat gttgttcgtc ttaataaaga
    15181 taaggaaact gtaaagaatg ctataatgca atatggttca gtgacatcag gttatgcaca
    15241 ttattcaaca tattttaata aagatgaaac agcatataac tgtacaaata agagagctcc
    15301 attaaatcac gctgtagcga tagtaggatg ggatgataat tattcaaaag ataattttgc
    15361 atctgatgta aaaccagaat caaatggagc atggttagta aaaagtagtt ggggagagtt
    15421 taattctatg aaaggattct tctggatttc ttatgaagat aaaactctac taacagatac
    15481 agataactat gcaatgaaat cagtatcaaa accagatagt gataaaaaaa tgtaccaact
    15541 tgaatatgct ggtcttagca agataatgtc aaataaagta acagcagcaa atgtatttga
    15601 ttttagcaga gactctgaaa aacttgattc tgttatgttt gaaacagact ctgtaggagc
    15661 aaaatatgaa gtatattatg caccagtggt aaatggagtt cctcaaaaca attcaatgac
    15721 aaaacttgca agtggaacag tatcatattc tggatacata aatgtaccta ctaattctta
    15781 cagcttacca aaaggtaaag gagcaatagt agtagttata gacaacacag caaatcctaa
    15841 tagagaaaaa tcaactttag catatgaaac taatatagat gcatattatt tatatgaggc
    15901 taaagcaaac ttaggtgaaa gctacatact tcaaaacaat aagtttgagg atataaatac
    15961 atatagtgaa ttttctcctt gtaactttgt tataaaagct ataacaaaaa catcttctgg
    16021 acaagctact tcaggagaat ctttaactgg agcagataga tatgaaacag cagttaaagt
    16081 tagtcaaaaa ggatggactt cttcacaaaa tgcagtatta gtaaatggag atgcaatagt
    16141 ggatgcttta acagctacac catttacagc agcaattgac tctccaattc ttttgacagg
    16201 aaaagataac ttagattcaa aaactaaggc agagttacaa agattaggaa ctaaaaaagt
    16261 ttatctaata ggtggagaaa attctttaag caagaatgta caaactcaac ttagtaatat
    16321 gggtatatca gtagaaagaa tttcaggtag tgatagatac aagaccagta tatctctagc
    16381 tcaaaagtta aatagtataa aatctgtttc acaagttgca gtggcaaatg gtgtaaatgg
    16441 acttgcagat gcaataagtg ttggtgcagc agctgctgat aacaatatgc caataatact
    16501 tactaatgaa aaaagtgagt tgcaaggtgc tgatgagttt ttaaattcat ctaaaataac
    16561 taagtcttat ataattggtg gaacagctac tttatcatca aatttagaaa gtaagctttc
    16621 aaatccaaca agacttgcag gcagtaatag aaatgaaact aatgctaaga taatagataa
    16681 attctaccct agctcagatt tgaaatatgc ttttgttgtt aaagatggtt caaagagtca
    16741 aggagacttg atagatggac tagcagtagg agcattgggt gctaaaactg attcaccagt
    16801 agttctagtt ggaaataagt tagatgaaag tcaaaaaaat gtacttaagt ctaagaaaat
    16861 agaaactcct attagagttg gtggaaatgg aaatgaaaat gcttttaatg aactaaatac
    16921 tcttttagga aaatagtgaa tggaaagata gtaaggatat acattttatt attaaaaaat
    16981 gttcaataga agtttataaa tataaaataa caaaaagggg tgtctcaaaa tgaacttttt
    17041 agttcatgag gcactctttt ttgttgtttt atatttttat catttaaata atgaaaaata
    17101 ggattgtcta tatttttaga tagttttctt attattgatg aaaaatgtaa taattttaaa
    17161 ctgtaaaata gtgtaaagtt attttataat ataaatatag taagatttta aacaaaataa
    17221 gggggaataa taatgaaagc accaaaaact attttaacaa tactgacaat agcgcttact
    17281 ttaagtagca tttctataat accatcatat gcacttacag aggaaaaatt aataggtaat
    17341 ggtagatatg aaactgctgt aaagataagt caaaaggcat acagttctag tcctaatgta
    17401 gtgctagtta atgacaattc attagctgat gcactttcag ctacaccatt tgcaaaggct
    17461 aaaggagcac caatactttt gacagaaagt gataagctag atgataggac tgaaaaagag
    17521 ataaaacgtc taggagctaa agatatttat ttgataggag gtactgctgt acttaataaa
    17581 gatatagaga acaagttaaa aagtaatgga cttaatgtag aaagaattaa tggtaaggat
    17641 agatatgaaa cttcactgat tttggctaat aagcttaagg atataaaaga tattaaggaa
    17701 gttgctgtag taaatggtga aaaaggattg agtgatgctg ttagcgttgg ggcaccagca
    17761 gctcaaaata agatgcctat aattttgtct agtcctaagg atggagtaga agcatttgat
    17821 aaatttataa gggatgagaa agttataaaa gcatatgtaa ttggaggtac aaattctgtt
    17881 tcaagagctg tagaaaagag tcttcctaat gcagaaagag tgagtgggaa agatagaaat
    17941 gagactaatg ctaaagttat agaaaagttt tacactgata caaatttaag taatttatat
    18001 gtaactaagg atggtagtaa aaatgagaat caactaatag actcgttagc tgttggcgta
    18061 ttggctgcaa aaaatgagtc tcctgttgtt ttggttggta ataaattaaa cacaaaacaa
    18121 agagatatac ttagtactaa aaaattaaat actataacac aggtaggtgg aaatggaaat
    18181 gaagaagctt ttgatgaaat taaaagtcta caagaaaaaa ctgtgtttga agcaaaaact
    18241 gttgaggaat taactgatat gataaatata gcaagtccta atgatattat aaactttaaa
    18301 ccaaaagaga atactgtaaa tgaagctttt agaatggtaa caaataaacc aataactgtg
    18361 aatataaagg gtgattgttc aaagactctt acagtagata tgcctaatgg tgaagtgaat
    18421 aactatgcta ctttggtaaa tgttatagtt agaaatattg gagaaggtgg atttaataat
    18481 catgatacaa tcactatatt gtcagttcgt gacaaaaatg gtagagttat agaaaatact
    18541 agaaattcag atatagatac gttgatgata ttagcttctg ctaatgatac aaaattgatt
    18601 aatgatggat atataggaaa acttatagat aattcatcta atagtgatat aactaataat
    18661 ggtactatag ataaaaaagt taatcaggtt gaagatttag aggctaaagt agattcaatt
    18721 gaaaaagcta tagattctat aagtcaaaaa gtcaataaaa tacaagatat actagataag
    18781 cttggatttc taaagaaatt tcttagctaa gaatatgtat atttaagtga agtgtgtgtt
    18841 gtaattgttc taaagttatg tagttaaaca ggtttatata caaatagatt tatagataaa
    18901 tatgtagttt agtatatatt tgaataagct ttggggaggt aacatgggga ttggaattga
    18961 atgtaacaaa aaaataaata aaagtattga caaaactaga ataaagaaaa ataatctaaa
    19021 tagcaatagc aatagcaata gcaatagcaa tagcaatagc aatagcaata gcaatagcaa
    19081 tagcaatagc aatagcaata gcaatagcaa tagcaatagc aatagcaata gcaataaaat
    19141 ttcaaataat gctgtagatt ctaaacttat gaatagtagg attaaatcaa tagatataat
    19201 aagagggcta tcaatagccc tcatgatagt ctgcaataat cctgggactt ggatgagaat
    19261 gtatcctcaa ttaagacatg ctgtatggca tggtgtaaca cttgcagatt ttgcatttcc
    19321 tttttttgtt atatctttag gtgttacaat tccaatatca ataaatagta aactaaaaaa
    19381 taataaatcc acactgagta taatacttag tatattcaag agaagtatct tattgatatt
    19441 atttggattt ttcttaaatt acctaggaaa tcctgattta gatactgtaa gaatacttgg
    19501 agtacttcaa cgtatgggat tagtctattt tgtgactagt ttagcatatt tactgctaaa
    19561 aaaattaaat gttggaagta ctgctacaat aattactttt ctatatatag ctacatttat
    19621 aatagttggg tactacatct tagcaaaacc ttatggattt gagctagaag gttctcttgc
    19681 acagttagtg gatttacatt tcttcaaagg acatttgtac aagccggaat ttgaaccaga
    19741 tggtttttta actagtatag tagcaatttc ttcaggtatg ttgggatgca ctatgggatg
    19801 tgtactttta aaagaaaacc taggagaata taaaaaattc tttaaaatac tggttatgag
    19861 cataatcctg ctaataggtg cttttatctt taatcagtat tttccattta ataaaagatt
    19921 atggtctagc tcatttgtac ttttaatggc tggcagttat ggtatattac tgtcaatatt
    19981 ttattttata tgcgacatca aaaataaaag taaaatattt accccaataa tagcacttgg
    20041 aagtagcccc atttttacat atatgtgcct tgagatattg agtcatgttt tttggaatgt
    20101 tccaaaactt acgaacaaag tcgattatcc aactaccttg gtagagtgga ctacatatga
    20161 acttataaca ccttgggcag gtacaacttg ggattctctt atattttctt tattgtatgt
    20221 cctattctgg gttatagtaa tgtctataat gtacaagaaa aaaatattta ttaaaattta
    20281 gtgcaatatt tttagatgat aaagtctttg atttgttcaa atatacagaa actatcaaat
    20341 ataatcaaaa tttctcctcg ataaaattta aaaaccatgc cataaaagat tacaaaaagt
    20401 ttacaatttg taattttttg tgctataatg aaaaaagatt tacataatat tgaatttatg
    20461 taatataaaa caagcttaag atgtattaaa aaagatttaa agatttaaag aaccaaaaaa
    20521 ttagggggga tatgatgaaa aaaacaacaa aattattggc aacaggaatg ttgtctgtag
    20581 caatggtggc accaaatgta gctttagcgg ctgaaaatac tactgcaaat acagaatcta
    20641 attcagatat taatataaat ttacaaagaa aaagtgtagt gttaggaagt aaatctaatg
    20701 caagtgtaaa atttaaagaa aaattaaatg ctgattcaat aacattaaat tttatgtgtt
    20761 atgatatgcc acttgaagct actttaaatt acaatgaaaa gacagattca tatgaaggtg
    20821 taataaacta taacaaagac ccagaatact taaatgtatg ggaacttcaa agcataaaaa
    20881 taaatggtaa agatgaacag aaagttttaa acaaagaaga cttagagtct atgggattaa
    20941 atcttaagga ttatgatgtt acacaagagt ttataataag tgatgcaaat tcaacaaaag
    21001 cagtaaatga atatatgaga aaaacttctg caccagtaaa aaaacttgct ggagctacta
    21061 gatttgaaac tgctgttgag ataagtaaac aaggttggaa agatgggtct agtaaggttg
    21121 taatagttaa tggagaatta gcagcagatg gaattacagc tactccactt gcatctacat
    21181 atgacgcacc aatattactt gcaaataaag atgatatacc agaaagtaca aaagcagagt
    21241 taaaacgtct aaatccaagt gatgtaataa ttataggtga tgatggttca gtttcacaaa
    21301 aggcagtgtc tcaaattaaa tctgctgtaa atgtaaatgt gactcgtatt ggtggagttg
    21361 atagacatga aacatcttta cttatagcaa aagaaataga taagtatcat gatgttaata
    21421 aaatatacat agcaaatgga tatgctggtg aatatgatgc tcttaatatt tcatcaaaag
    21481 ctggtgaaga ccaacaacct ataatattag ctaataaaga ttctgtacca caaggtacat
    21541 ataattggtt atcttcacaa ggtttagaag aagcttatta cataggtggt agtcaaagtt
    21601 tatcatcaaa aattatagac caaatatcta aaatagcaaa aaatggtact tcaaaaaata
    21661 gagtttctgg agcagataga catgaaacaa atgcaaatgt aattaagact ttctatccag
    21721 ataaagagtt gagtgcaatg ttagttgcaa aatctgacat aatagttgat tcaataacag
    21781 caggaccttt agcagctaaa ttaaaagctc caatacttat aactccaaaa acttatgttt
    21841 ctgcatacca taatacaaat ttatcagaaa aaacagcaga aacagtatat caaattggtg
    21901 atggaatgaa agatagtgtt ataaattcta tagcatctag tttatcaaaa cacaatgcac
    21961 caacagaacc agataattca ggttcagcag caggaaaaac tgtagtgata gacccaggtc
    22021 atgggggttc tgattcaggg gcaacaagtg gattaaatgg tggagcacaa gaaaagaaat
    22081 atactcttaa tacagcatta gcaactactg aatatttacg ttcaaaaggt ataaatgttg
    22141 ttatgactag agatacagat aaaacaatgg ctttaggaga aagaacagca ttatcaaata
    22201 ctataaagcc agatttattt acaagtatac attacaatgc gtctaatgga gctggtaatg
    22261 gtgtggaaat ttattacaaa gtaaaagata aaaatggtgg aacaactaag actgcagcat
    22321 caaatatctt aaaaagaata ctagaaaaat ttaacatgaa aaatagagga atcaagacaa
    22381 gaacacttga taatggaaaa gattacctat atgtgttaag aaataacaat tatccagcaa
    22441 tacttgttga atgtgcattt attgacaata agagcgatat ggataagtta aatactgcag
    22501 aaaaagtaaa aacaatggga actcaaattg gaataggaat agaagataca gtaaaataaa
    22561 atagaaaata ttaaagcaaa acatatcatc tcctgttata atgtaaaata taacaagaga
    22621 tgatatattt ttgtaaagaa tcaaattgtt tacaatgatg tttcttgtat tgataatctg
    22681 ctcattattg agtataatat agaaataagt caaaaataag atagaaataa gttgaaatta
    22741 agaattaaaa aaatttttat tccaatatgg aggtaactat gtctggatat actaatgatg
    22801 agtgtgaaat acctaaaatt aaaagctatc caggggcaga taaagaaatt gcaagtgaga
    22861 ttgactatag catagtaaaa ggaacagttt tattcgactt gtaccaaaga attatggatt
    22921 tagttttatc tataatagga cttgtgatag gtttaccttt aatagcaata tttggaattc
    22981 ttattaaaat agaggacaaa ggaccaataa cttataagca agaaagacta ggaaaatgcg
    23041 ggagaagatt ctatatatat aaattgagat ctatgagaac agacgcagaa aaatttggcg
    23101 cacaatgggc tgaaaaagat gatcctagaa taactaaggt tggaaagttt attaggaaaa
    23161 ctagaattga tgagattcca cagctgttta atatattaaa gggggacatg ggcttaatag
    23221 gtcctagacc agagagaccg aactttactg ttcaatttaa tgaggaaata cctggattta
    23281 tcaacaggct cgctataaaa cctgggctta caggttgggc acaagtaaat ggtggatacg
    23341 aaatcactcc tgaagaaaaa cttaaagaag atatttacta tataaaaaat aggagtatat
    23401 tacttgactt taagatactc tttaaaactg ttaaagtagt attgacagga gacggagcta
    23461 gataatttta aaatattaga ttactaatca atgaaaaagc tctttaaaaa gagctttttt
    23521 attgtcaaaa aatacaaaaa agaaaaggaa tgagaactta tgattaaaaa aatttctact
    23581 atattatcgt tagttttgtt aatctctatt tctagtacga taggagtatt tgcagatgca
    23641 aatcccaaaa gagaattgat agaagggagt ataccagaga tttcgacaga attaaataaa
    23701 agagcattta aggattctaa agaagtaatt cttgtaaatg aagagtcaat tgtagatagt
    23761 ataagtgcaa cacctttagc atatgcaaaa aatgctccaa tagttgtaac taagagtaaa
    23821 aatcttggta gagtaacaag aaattatcta aaagaactag gtccagagaa ggttacgata
    23881 gtaggtggtt taaaggcagt atctaaagat gctgaacgta atatagaaaa aatgggaatg
    23941 aaagtagaaa gaataagagg gaaagacaga tatgatacat ctttaaagat agctagagaa
    24001 atgtatagaa cagtaggatt tgatgaagca tttttactta gctcaacaac aggattagag
    24061 aacgccatat ctgtatattc ttatgccgct aaaagtggaa tgccaataat atgggctaaa
    24121 gatgaagggt ttgaggaaca aattgatttc ttaaaaggca aaaatcttaa aaaaatatat
    24181 gcattaggtg attcaaaaga gtttattgca gagattgatt ctaacttaaa aaatatagaa
    24241 ggaataaagc aaataaataa gtcaagtaca aatgttgatt taataaagaa gttttatgat
    24301 gaaaaagata taaagaaaat ctatactgct agattagact ttggtagtag atctgatgta
    24361 aacgaatata tttctttggg agtagtatca gcaaaagaaa atatgccaat actgatttgt
    24421 agtgataact taagtcgtgc acaagataaa ttcttgaaag atagcaatat aaatgatgta
    24481 gttgaagttg ggtatacagt aggtgattat agtctattta agagcatatt taatctaaca
    24541 ttcttatctt gtatagtatt aatactatta ttactattga ttacatttag ggcacttaga
    24601 tatgagtcga aatagttttt gatttataag gaagcttttc ataggttaaa tttacttaat
    24661 tagaataatg tttataagca gttttaaaag ttctactttt agagtttaaa ttagataagg
    24721 agaaagaaga tgtcaaaaac agctaaagca gcattgtgga ttatggcagc cactatgttt
    24781 tcaaaagtat taggtttcct tagagaactt gtacttgcaa atttctatgg gacaggtatg
    24841 tatgcagacg tatttgtatt aacattaaat atacctggtt taatcatagc agtcataggc
    24901 tctgcagttg ctacaactta tatacctatg tactttgaga caaagaaaag actcggagat
    24961 gaaggtgcct taaaatttac taataatgtt ttaaatatat gctatataat ggctatagta
    25021 atagctatta ttggtctttt atttacagag caatttgtta cagtatttgc agcaggattt
    25081 agaaacgacc ctgctaagtt ccaagcagca atattattta ctaaaataat gatttcagga
    25141 gttttattcc tgagtggaag taagatattt agttcgtttt tacaggtgaa tgacagtttt
    25201 gtaattcctg ggcttatagg aatcccatat aatattataa taatagcagc aatagcacta
    25261 agtgcaggta aaaatgtatg gatactgcca gcaggagcat tattggctat ggcgagtcag
    25321 ctattgtttc aactgccatt tgcgtttaag aagtcctaca agtataagcc atatataaat
    25381 ttaaaggatg agtctataaa agagttagtc aacctagtat tgcctatgtt ggtaggagta
    25441 gctgttggac agttaaatat atttgttgac cgattattgg caaccacttt gggtgatgga
    25501 aagttgtctg ctcttaacta tgcaaatagg ctaaatgagt ttgttatggc attatttgtt
    25561 acatctataa taacagttat atatcctaaa ctagcaaaaa tgtctggtaa agataataaa
    25621 gaaggattta taagcactat tgtaaaatct tcaaattgta ttatattggt ggtgcttcct
    25681 atatctatag gagctattat attagcagag cctcttgtta gaatattatt ccaaaggggt
    25741 aaatttgatg cgttatcaac agaccttaca tctatagcgc ttagattata ttcactaggt
    25801 ttattggctt gtggagtaag agatgtctta tatagggcat tttactctct ttcagataca
    25861 aaaacaccaa tgataaatgg aagtatagct ttaataataa atatagttct taatttaata
    25921 ttgataagac cattgggtca tgcaggtatt gctatatcaa ctagtacatc aaacataatt
    25981 acagttatat tattatttat atctcttaaa aagaaaaatg gatattttgg aggggataaa
    26041 attataaaga ctggtctaaa aagtttggta gcatctggtg tgatggcagt agctacttta
    26101 ttgatatata ataatctata tgcatttatg ggttcaggaa caattaaaga aattatatca
    26161 gttggagctg gagtattagg tggagcttct gtatatactg tattgatagt tttatttaag
    26221 gtagaagaga tggatttagc ttttgaattt ttaagaaaag gaaaacaaaa actacttaga
    26281 agatagataa ttaccttgaa attttaaatt agagtcagaa aggatgaaaa tatggattat
    26341 aagaataatt atgagatgtg gttaaattct ccttattttg atgaacaaac gaaaaatgag
    26401 ctattaagta taaaagatga tgaaaaagaa atacaagata gattttataa aaatttagag
    26461 tttggaacag gtggtttaag aggtataata ggagcgggaa ctaatagaat caatatttat
    26521 actgttagac gtgcgacact gggcgtttta aattatatca tgaaaactca aggagaagaa
    26581 ggtaagcaaa agggaatagt tatagcacat gatagtagat acatgtctag ggagttttgt
    26641 atagaagtag caaagacttt aagtgcttat ggtgtaaagg cttatatttt tgaagaatta
    26701 aagcctacac cagaactttc ttttgctgtt agatatttaa agtgtgctat gggaatagta
    26761 ataacagcaa gtcataatcc taaagaatac aatggatata aagtatatga ctcagatggt
    26821 ggtcaaatct gtatagatat ggcaaatgat ataatagcag aagttaataa gattgatgat
    26881 tacagtacta ttaaaagtat agattttgaa gaagctttat ctaaaaattt aataactata
    26941 ttagataatg aagtagatga tgagtttata aaagctgtta agaaacaagt tttaagacaa
    27001 aatataatag atgaatacgg gaaaaaatta aaaataatat atacacctat tcatggtaca
    27061 ggaaataagc cagtaagaaa agtcttaaat gaatgtggtt ttgaaaatgt aatggtagtt
    27121 aaggagcaag aattgccaga ttctaatttt tcaacagtaa agtatcctaa tcctgaagaa
    27181 aagtcagtgt ttaatatagc tattgaaatg gcaaagaata atggcactga tttgataata
    27241 ggaacagacc ctgattgtga tagagtaggt atcgttgtaa aagattcaag tggtgaatat
    27301 gtagtcttaa atggaaatca agtcggttca cttttagtta gatatatatt agagagttta
    27361 gttgaagaaa ataaacttcc taaaaacaat cctacaataa taaaaactat agttacatca
    27421 gaactaggtg caaaaatagc aaaggcttat aatgtagatt gtttaaatac tttaacagga
    27481 tttaaattta taggagaaaa aataaaagcg tttgaagaaa gcaatgatag aagttttata
    27541 atgggatatg aggaaagcta tggttactta attggaactc atgctagaga taaggatggt
    27601 gttgtatctt cacttatgat atgtgaaatg gcagcatact attcttcaaa aggtatgaac
    27661 ttgtatgaag ctttgataga tacatataat aaatttggat actacaaaga ggacttaaag
    27721 tcagtaactt taaaaggcat agatggaata aaaaaaataa aagagatgat gctttacttt
    27781 agaagtgtta aaatagataa tgtggctgat gtaaaagtag ataaaatttt agactataaa
    27841 gatggtgttg atgatttacc taagtcagat gtacttaaat ttttacttga agatggctct
    27901 tggatagcta taagaccttc aggtacagaa cctaaaatta aattttactt tggagcaaat
    27961 tcagacaatc aagaagatgt agaatttaag ttgaacaatt taatatctta tatattaaat
    28021 gtagtggatt ctatataaaa agctaagggg agataaacaa tgaaagtata caatgtcata
    28081 atggctggag gaggaggaac tcgtttctgg ccacttagta gacaagaagt acctaaacag
    28141 cttataaatt taagtggaga agatgcttta ataaacgaaa ctataaacag aatagattct
    28201 ctagcaaaaa aagatgattt atttatagta accaatgaga agcaattgga agctttaaag
    28261 gatatagtaa aagataaatg tttggatagt aatatattgc cagagccatg tgccagaaat
    28321 acagcagcag ctataggctt tgcagcattt aacataatga aaaaatatgg tgatggtgtt
    28381 atgtgtgtat atccagctga ccattatata aaagatgaaa aagaatttaa atctattttg
    28441 gaaaaagcta tctatatagc agaaaataat gataaactag taactattgg gataactcct
    28501 acatttccat caacaggata tggatacata aattttaata gagaaaatac tatagaagat
    28561 gttgcatatg aggtagtgga gtttgtagaa aaaccaaact atgaaattgc taaagaatat
    28621 gtaaactcta aaaagtatgt atggaatagt ggaatgttcg tatggaaggt atctaaaata
    28681 ctagaagatt ttaagagata cttgccaaaa gtatatgaaa aattagaaga tataagtaaa
    28741 tacttaggaa caaaagaaga aatggaaaaa ataaaagaaa tatacccaac tatacaatca
    28801 atctctatag attatggtat tatggaaaga agtaatgatg ttatagtagt tcctggtgat
    28861 tttggatgga atgatgttgg ttcttgggat tctctaggtg caatatatcc aactgatgat
    28921 gaaggtaata tcaaaagagg tgaaaatatt actatagata ctaaaaattc aattatatat
    28981 tcagatgaca aattaatatc aacaataggt attagtgatt tgatagtagt atctacaaat
    29041 gatgcagtta tggtttgtag aaaagataaa gctcaagatg tcaaaaaaat agtagagcag
    29101 ttaaaagaag aagatagaca agaatatatg taggaggtgt tcatttgatt cctaaaaaaa
    29161 tacattatgt atggtttggt ggtccaaaag gaaatataga aaatatatgt ataaattctt
    29221 ggaaagaaaa gcttccagaa tatgagatag tagagtggaa tgaaaaaaac tttgatatag
    29281 agaaagaaat aaaaggaaat aagtttttgg aagaatgtta taaaagaaaa ctttgggcat
    29341 tcatatctga ttatacaaga ataaaagtac tctatgaaca aggtggagtt tatatggata
    29401 cagatatgca aatattaaag gatataactc ctcttttaga aaataatagg ctagtctgtg
    29461 ggtatgaaga tgatagagag tatataaatg gtgctataat aggtgtagaa aaaggacatc
    29521 cttttttaaa agacttattg gagtattatg aaaaagaagt acttacttct tcattgttta
    29581 caataccaaa gataatgact catcttatgg aaaagaatta taagaagatt gatccaaata
    29641 attatgaaga aggaatacgt gtttacgaca aagaatattt ttatcctttt ggatttaaag
    29701 aagactttac accagaatgt ataacagaaa acacttttgg gatacattgg tggggaaaaa
    29761 gttgggctaa aaagagaaat tatttcttag aaagcaaaca cttaacaggt gtaaataaga
    29821 tatggaagtg ttgtaaaata tttgcaagta atactttacg aagctaaaag ggagaaagta
    29881 aaatgaagac tttaaatgat gatagaaata ttatgattag aaaatatctt tatatatttg
    29941 ttttgtttat catattactt agaaagtata tgatatcatt tattgaccca aatatggata
    30001 tagggatgat taaatcagcg ttattttatt cttcaatagg aatactaatg ctactttttt
    30061 tatttgataa gagaaaaagt ataacagaaa tggtactagt tggagtttgt gtcttacttt
    30121 atttattaaa tagggaagga gctatattat taatcgtttt attagctgta tcagctaagc
    30181 aaatagatga taaatttata gttaaaaatt atttaataat atcggcatgt tttttaatgg
    30241 tagctatatt attatttaat ttatttcctt cattaatatt taatcaggaa gtccactata
    30301 gatatataga aaaaatagat atgcttgtta caagaatgga ttttggactt ggtaatccaa
    30361 atagtgtatt ttatcatatg gttactatat atgcagcata tatattctta agatataaag
    30421 attataataa atgggataga atacttttat ttggttctgc attttttgtt tatcaaacca
    30481 catatagtag aactggattt ttcactatat tagcaggatt aatatttgta gaaataataa
    30541 gatggataga tataaaaaaa ataaaaggac taccaatgct cctaaagaca ttaccggtaa
    30601 tacttacttt atttagtgta attattggta cagtatttga taaaagcact ttattaaata
    30661 gattattagc aagtagacct aagttctgga atgtatactt ggctgaaaag gggaactttc
    30721 taaaaccttt tggaaattca tatagtcctg ctataaaagc aactaatcca ttagatagtt
    30781 cttatgtata tataattagt gtactaggag tagttgcttg tatattattt atgtatttga
    30841 tgtataaagg aatagaaagc tttatagaaa aagataaaaa agcatattta gtagctttat
    30901 ttatattttt actatactct tttgcagaaa atttattatt agaagcttca tttagttttg
    30961 cagtagtatt actaataaaa gaagtgatat taaatgataa aagggaaata gatttgtgga
    31021 agatgaaaag taggaggtag taatgttaat atcattaata atgccaacat taaatagata
    31081 tgatgatata tacttactta tggacagctt agaaaatcaa acttacaaaa actttgaact
    31141 tatagtagta gaccaaaatg ataatagcaa agttaaagaa atagttgata aatacattga
    31201 taagttagat ataaagtata taaaaagctc taaaaaagga ttgagttata acagaaatgt
    31261 aggcatagac aatgctgtag ggcaaattat aggtttccct gatgatgatt gtgtctacga
    31321 aaatgataca ttagaaaaag ttataaattt ctttaataaa aataaagatt ataaaatata
    31381 cagttgcaaa actatggatt caaataaagt tgatgcattt aagaaaatgt atgatggaac
    31441 ttgtgatatc acaagttcca acgttttgga tactataaca tccataactt tttttattga
    31501 ttttgaaggt aaagactata caagatttga tgaaaaattg ggtgttggtg gagagtttgg
    31561 tgctggtgaa gagatagatt atgttttaaa cttattgagt ttaggtttta aaggaaaata
    31621 ctttggaaat gatataattt accatcctgc aaaaaaacat tctaaatcta aagaaaagta
    31681 tcaaaaagat tataactatg gaagaggctt tggagcgctt tgcaaaaagg aaatagtcta
    31741 tagaaaaaat tataagtttg caaaagttat ggtatcaaaa cttgtaagaa atataggagg
    31801 tcttatattg agttccaata gagattatca tagtgccact ataaaaggaa gaattaatgg
    31861 atttagacag tacaagttat aatgaggatg gtgtttatgg acaaggttaa atttttaata
    31921 aatatgcttt tggctttttt tatatatcca tttaataagc ataagtttaa tggtagagat
    31981 atctggctta taggtggaca ttcaggggat atatacaatg ataattcaaa gtttttttat
    32041 gagtatatgc taaaagaaca taatgatgta gaaacttatt gggtagtaaa taaagacagt
    32101 aaagtatttg ataaaattcc aggcaaaaaa ttaataagag gaagtgtaga aaactatctg
    32161 tattattaca attcaaaagc tatagtattt tcacatgcac catcagcaga tatagcacca
    32221 tacaattttg ctgtacctgt tcttaattat tttcataaaa aaacgataaa agtgttttta
    32281 aatcatggga ctataagttt taaaaaaaga aaacctatga acaaaaagtt caaaaatatt
    32341 atagataacc tatataaaag ctataatata gttacagcaa gttctgaatt tgaaagaaat
    32401 gttatggtaa atgattgggg aatgttagat gactcagttt atataattgg aaatgcaaga
    32461 tatgacaatt taccaacaaa tgaagttgca caaactcgag atatattata tactcctaca
    32521 tggagagatt ggataaaatt tagttcagga aaatttacgg atacagatta ttttaaaaat
    32581 ataatgaatt ttttaaatga tgacaaatta aataaaatat tagatgagaa agatataaat
    32641 gtaaaaattt atatgcatca tcttatgcat gagtttatag atgatataaa agaaaatata
    32701 acaggaaaac gaattgtgtt tttagataaa ggggtaactc tagcaaatga aatcagaaaa
    32761 tcagctgcaa atataactga ttattcaagt gtagccatag actttttgta tatgaataga
    32821 cctatcttgt tttaccaatt cgatttggat gaatatatgg aaaaagttga ttcatatata
    32881 gatttaaaaa gtgaaatgtt tgggtctttg gcttataata atgatgaagc cgtgaataaa
    32941 cttattgata ttattgaaaa taattttgag gttatggata accaaaaaaa tgaaagaaat
    33001 aagtttttca gatataatga taataagaac tgcaagagga tatatgattg tgtattaagt
    33061 aaaattaaat aaatatttta taaatgatga ggaaggtaat atgaagaaga atttagtatc
    33121 tataattact cccatgtata attctgaaaa atttattgaa gcaaccataa aatcagtatt
    33181 aaaccaaact tatcaagaat gggaaatgtt aattattgat gattgctcaa cagataatag
    33241 tcctaatata gtcaaatctt atatgcaaca ggatagtaga ataaaatgta taaagactga
    33301 gactaataag ggtgtctcta atgctagaaa tttagcacta agtaaggcaa caggacaatt
    33361 tatagctttt ttagatagtg atgaccaatg gaatagtagt aagttagaaa aacaagtaaa
    33421 ttttatgtta gaaaatgact atgtaatttc atttacttca tatgaactga tggatgaaaa
    33481 tgataaaaaa ttaaacaaag taataaaagt tccacctaat gtagattata gaagattatt
    33541 aaaaggaaat atcttaggat gtcttactgt tgtgattgat aagtctaaat tggattttga
    33601 aataagaatg agtggtgtaa gacatgaaga ttatgtttta tggttatcga tattgaaaaa
    33661 agggcatatt gctcatggaa taaatgaagt tctagcatta tatagaaaat ctagcaattc
    33721 cctaagtggt aataaaataa aagctgctat gtggacttgg aatatatata gaaatataga
    33781 aaaaatacct ttatacaaag ctatatacta ctttattaat tatggaataa acggaatcaa
    33841 aaaaagttag acagagagga agatttaatt aatgtataaa tataaattta ctgtatttac
    33901 tccgacgtat aacagagctc atttacttga aaatttgtac aatgatttaa aagctcaaac
    33961 ttatgatttc aatgattttg aatggttgat tgttgatgat ggaagtagcg acagtactaa
    34021 agagttagtt gaaaaattta tatcagaaaa taaattaaat ataagatata tatacaaaga
    34081 aaatggagga aaacatactg caataaatct aggtgtaaaa aatgcagatg gagagctatt
    34141 tttcattgta gatagtgatg atggtttaat taaagattct cttgaaattg ctaatgatga
    34201 gtgggattct ttagagaata aagaaggatt tagtggagtt gttggacttt gtgtatatcc
    34261 aagtggagat ttgataggga cagaaatgcc tgaagataag aaaatatgtc actttgcaga
    34321 cctatatttt aagtatggcg ttaagggaga taaaagcata gtatttgtaa ctgatgagct
    34381 tataaaattt ccttttccag aaagaaaaga agtgagattt ttaccagaaa gtgtagtatg
    34441 gaatgaaatg tctaaatact ataaagttat atgtgtaaat aaacctatga taataagaga
    34501 ttatttggat gatggtttga ctaagaatat attaagtaaa aatgcattaa gaggtagagc
    34561 attagagttt ttatatttaa taaatcaaaa tacatatcca ttaagtagat atccatatat
    34621 gtggataaaa aattacataa atttagccag atactcattg ttgtctgata gtcattattt
    34681 tggtgaatta agaaaagtat ctgataaatt gttgtattta gcattattcc cattaggata
    34741 ttacaaatat attggacaaa gaaaattagt atctaagtaa acttgttggt atagtactgc
    34801 tagttgctat ataaggagag taaaaatatg aatgagcctt tagtatcgat aattactcct
    34861 gtttataatt cagaagagtt tttatcagag acgataaagt caatccaaaa tcaaacttat
    34921 aaaaattggc agcttttatt agtggatgat tgttctaagg acaattcaag tgaaattata
    34981 aaaagttttc gaaaagaaga tgctagaata aaatatataa agcttgaaaa aaattctgga
    35041 gctgcagtat ctagaaatgt tggtattaag aatgcggaag gtcgatttat tgcatttgta
    35101 gatagtgatg atttatggga tagtagaaaa ttagagattc agatagaata tatgttaaaa
    35161 gaaaatgtag gtttttcttt tacatcttat agatatatga gacaagatgg gtcaaaaacg
    35221 aataaggttg caagagctcc taagaaaatt gactatgaag gattacttag aaatactata
    35281 ataggatgtt ctacagtggt cattgataag gagattgtag gagagttcag catgcctttg
    35341 gtaagaagag gacaagatac tgcaacctgg ttacagttac ttaaaaagga aaaatatgca
    35401 tatgggattc aagaagattt agtgaattac agattagttg gaaattctat atcaagtaac
    35461 aagataaaag cattaaaacg aacttggaat acttatagaa atgttgaaaa tttaagttta
    35521 ccaaaaagtc tatatgtatt ttgtttctat gtttttaatg ccattaaaaa aagagtataa
    35581 gttttatata cttggaggta aaaaaatgaa aatagctata gctgggacgg gatatgtagg
    35641 tttggttaca ggtgtttgtc tagcagaggt aggcaatgaa aatgtagtct gtgttgatgt
    35701 agatgaacaa aaagttaaat caatgaaact tggaatagca cctatatatg aagaaggcct
    35761 agaagaactt atggttaaaa attacaacag aggaagaata gattacacta cagactataa
    35821 aaaagcttat gaagatgtag atgtaatatt aatagctgta ggtacacctg agagaagtga
    35881 tggttcagcc aacctagatt atataaaggc agttgcaaag caaatagctg aaactgtgac
    35941 aaaagatact ctagtagtaa taaaatctac agttccaatt ggtacaaatg aagagataga
    36001 agattatata aatagaaacc taaaaaatga tgtaaaagta gaacttgcat ctaatccaga
    36061 gtttttatca caaggaacag cagtaaaaga tactctacat gcttcaagaa tcgttatagg
    36121 tgtagaaagt gaatgggcta gagaaatatt agaggaaatc tataagcctt ttaatcaacc
    36181 tataatagtt acaaatagaa atagtgctga gatgataaaa tacgcatcaa ataacttctt
    36241 agctcttaaa atatcattta tgaatgatat tgcaaatctt tgtgaaatag caggagctaa
    36301 catagaagat gtagctttag gaatgagtta tgatgataga ataggaagta agtttttaaa
    36361 ggcgggaata ggatatggtg gttcttgctt tccaaaagac acaaaagctc ttcactggtt
    36421 atctgaacat aatggttatg aattaaaaac agtaaaagct gcaatagaag ttaatgaaag
    36481 tcaaaaactt aaattagtta gaaaggcatc taagttagta gagagctttc aagatatgaa
    36541 ggttgctgtt ttaggtttga cttttaagcc agacactgat gatataagag aagcaccatc
    36601 tataccaaat ataaaatatt tgctcgaaag aggggcaaga gttactgtat acgacccaat
    36661 tggtgtaaat aatactaaga aaatatttgg tgactctata ctttatgctg attctgtaga
    36721 tgatgcgata gaagatgcag agatatgttt tataatgaca gaatggaaag atgtattaaa
    36781 ttatgatata cataagtata aggaaaagat gaaaaatcct ttagtccttg atggaagaaa
    36841 ttgttataaa attaaagata tggaagagtt aggaattgag tattattcga taggaaggta
    36901 aatattatga agactgtttg ttatttagct gatgggtcaa atcctcatac tttaaaatgg
    36961 tgtagttttt ttaaaaataa aggatatgac attcatgtaa tctctttaaa tggaggtcac
    37021 atggaaggaa taaaaattta taattttggt tctaatgtag aagaactaaa aaatgaaaat
    37081 atttctaaaa aaacgggata cttaggttca ataaagcaga taaagaagct agtaaatgaa
    37141 ataaagccag atatattgca tgcacagtat gcaagtagtt atggatttat aggaagtcta
    37201 cttgggtatc atccatatat tatttctgtt tggggtactg atatttatga tttccctaat
    37261 aatggtttta tacaaaaaca aattataaaa cataacttta gaaaagcaga ctatatcttt
    37321 tcaaatagta gagatatggc taaagaggct aataaatata cagctaaaca tgtagatgta
    37381 accttttttg gagtagatat ggataggttt aagcctatgg aagtagaaaa agaagacgct
    37441 tttgtaatag gaattataaa gagtttagaa aaaaaatatg gaatagaata tttaatacaa
    37501 gcatttaaga tgcttaaaga tgaatataaa gataagaaaa taattcttaa aataggtggc
    37561 tctggaagtc agatggacaa cctaataaat ttagctaaag aattgggtat agaaaatgat
    37621 gttcagtttt taggaagaat atctccagaa aatgtaagca agacttttaa ttcttttgat
    37681 gtaactgtat ttccttcttt aagagaggga tttggagtgg cagctataga atcagaagca
    37741 tgtgaagtac ctgttatagt tacaaatgta ggaggacatc cagaatctgt atgggaaaat
    37801 gaaactggct taattgtaga accaaaacaa ccagaagaaa taaaaaatgc tataataaaa
    37861 ctaatggaaa atgatgaact tagacttaat atgggtaaga aagggcgtca atttgttaga
    37921 gaaaattatg aggttaattt aaactttaat gatatagaaa aaatttatga ttctatattt
    37981 gataaatata aaaaataaaa tgaaaaaata cagcaaaaga tagatgttgt tggctatgct
    38041 taggtgcgta gcttttgctg tttaaaggag ggcttctagt tatgcgagaa agtatagatt
    38101 tagagaaaga aagctttttt aataggcttt ttaaaaaata taaatttaga catttaacac
    38161 tattaattct agatatttgt agcgtactag tagcgtttcg aatgtcttta agcttgacag
    38221 gtaatttaaa tgcatttacc actaatgata taataatttc agtgtgtata tatatagtac
    38281 ttcatatagt atcatttaga ttgtttaagt gctacaacac attatggaga tatgcaggag
    38341 aagaagaaat tatatctatt tttgtggcaa ctttagctta tttaattccg atttatataa
    38401 taaataaatt attagggttt gattatccta taatgtttta tgtattaaat acaatattta
    38461 ttataatgtt tacaagtggc gcaagaatag cctatagagc tataagaata gtcatgaaca
    38521 agacatattc tagaggaaag gtttcaaata tccttataat tggtgcagga gatgcaggag
    38581 aaatggttat acaggaacta aaaagaaatc cagaacttaa aaaagtagca gtggctataa
    38641 ttgatgatga taaaaataaa attggaagaa taatccataa tgtgaaaatt gtaggaacaa
    38701 cttcagatat aaaagatgtg gtagaaaagt acaatgtaga tgagataatc ttttcaatag
    38761 caaacataga gaaacgtaga aaaaaagata ttattgatat atgtaaaaac actaactgta
    38821 aaataaagac aataccagga atttacgaaa ttatagatgg aaaagttgat ataaaacaga
    38881 ttagagaagt agagatagaa gacttgttgg gtagagagcc tataaaaaca aatttaagag
    38941 agataagtaa ttacattgag ggaaaagtaa tcctaataac tggaggagga ggctcaattg
    39001 gttcagagct ttgtagacag attgcaggat ttaatcctaa agagcttatt atagttgata
    39061 attatgaaaa taatgcttac tcaatacaac aagaattact tagaaaatat aaaaataaat
    39121 tagatttgaa gacagtaata gcatcaataa gagaagaaaa aagaatggat gaaatattta
    39181 ataaatataa accagaagtg gtatttcatg cagcagcaca taaacatgtt cctcttatgg
    39241 aatcgagccc tggcgaagct ataaagaata atatatttgg aactttaaac atagctggct
    39301 tgtcttcaaa atatagagct aagaaatttg tccttatatc tacagataag gctgtaaacc
    39361 ctactaacat aatgggagct actaaaagag ctgctgaaat gattatacag actatgaatg
    39421 ctgaaagcca gactgaattt gttgctgtta gatttggaaa tgtgcttggt agtaatggta
    39481 gtgtaattcc actttttaag aagcaaattg aagatggcgg accagtgaca gttactcatc
    39541 cagatattat tagatatttt atgacaatac cagaagcagt aggcttagtt atacaagctg
    39601 gggctatggc taaaggtgga gaaatatttg tgcttgatat gggagaacct gtaagaatat
    39661 tggatttagc aaaaaactta attaaattca gtggatttga gccagatgtt gatataaaaa
    39721 ttgaattttc tggtcttaga ccaggagaaa agttgtatga agaactactt atgtcagaag
    39781 aaggtctatt ggatacagag cataaaaaga tttttattgg tagaccaata gatgttgata
    39841 gagaaaaaat aacaaaatac ttaaaacttt taagagaaat tactaacaat gaagaggttg
    39901 aaaaaataga tggtattatg agggaattag taccaactta tattaaacca gaagatgcta
    39961 atatcaaaga aattgcaaca actagagaaa agtagaatat atttctaatt tgtaaccagg
    40021 gggagcaaaa agaaacaaat atatactaaa aagaaacaaa agattacaaa aagattacaa
    40081 aaagattaca aaaaaggttg caatttattt tgatgtaaga tagaatgtta attaggaatt
    40141 ggaaacgaag gaacaaaatg ttaaattttc gaaaggatta ggtatttgag attatgatga
    40201 atattaaaaa taaaaaacat atattgaaaa aatttatagc tatggtgtta atagcaggag
    40261 ttgtcacagt agaagcagga gcaataacgg ctagtgcagc agagccaact aattcaccta
    40321 tgagtgcaac agtagaccag tgtgattttt taaatgtaag aagtggagct agtgcaaatg
    40381 atgcagtagt aggaaagata aacacaggag acaaagttga agttttagaa ttacattcaa
    40441 atggatggat taaaataaaa agtgttgaca atgttacagg atgggtaaat ggagattatt
    40501 taactattca aggtggaaat gtagatgcaa aggtacaaaa tgttttaaac ttagcattta
    40561 agcaacaagg taagccatat aaatggggag caactggacc aaattctttt gattgttctg
    40621 ggttcacttc ttatgtctat aaaaatggag caggagttaa tttaccaaga gtatcaagat
    40681 cacaagcaac agtaggaaag aaagtatcaa gagcagaatt aaaaccagga gatttagttt
    40741 tctttggttc aggtggttca ataaatcacg ttggattgta tgtaggagat tctaaattca
    40801 tacattcacc acaaacagga gatgtagtaa aagttactag catggctcca ggaactaatt
    40861 atgcaaaaag acttataact gctacacgtg ttttacaata ataatagata ttgatttaca
    40921 taggattgaa tattaacatt aaatatataa ttacatttaa tgttttacat agagatttaa
    40981 gagtttagta atattaaaat agtgatgaac gttataaaaa cagcaaagaa gataaatttc
    41041 tttgctgttt tttttataat aaattatttc atattataat caaatatcaa atataaattt
    41101 taactatatt attcaatatt tgacagataa tatgaatttt agatatactt aataagaagt
    41161 atattgtaga aaaaataaat ttaaaaaatt aagggggatg aaatgaaaaa aataagtata
    41221 ttagtattat cattgataat gacacttact atgtgtagtg taagttcatt tgctgattca
    41281 tcaaatgata aggaaatgag agctgcttgg atatctacag tttataattt agactggcct
    41341 aagactaaga ataatgaagc caagcaaaaa aaggaataca cagatttatt ggacaaatta
    41401 aaaagtgtag gaataaatac agcagtggtt caagtaagac caaaatcaga tgctttatat
    41461 aaatcaaata taaacccatg gtctgaatat cttaccggta cacaaggtaa agaccctgga
    41521 tatgacccat taccattttt aatagaagaa gcacataaaa gaggaatgga atttcatgca
    41581 tggtttaatc catatagaat aactatggca gatgagagca tagataaatt gccagcaaac
    41641 catccagcaa agaaaaatcc aagctgggta gttaaacatg gaaataagta ttactacgac
    41701 ccaggtttac cagaagttag aaagtacata gtggatagta tagcagaagt agttcaaaat
    41761 tacgatatag atggagttca ttttgatgat tacttctatc caggagtaag tttcaatgat
    41821 actgcaacat accaaaaata tggaaaagga caaaataagg atgattggag aagagaaaat
    41881 gtaaatactc tacttagaga tgtaaaagcg tctataaaga gtataaagcc taatgttgta
    41941 tttggtgtaa gcccagcagg tatatggaga aataagagta gtgacccaac aggttcagat
    42001 acatcaggta atgaaagtta tgtaggtaca tatgcagata caagagcatg gataaaacaa
    42061 ggattgattg actatgtagt tccacaactt tactggccaa ttggtcttaa agcagcagat
    42121 tattctaaat tagtggcatg gtgggctaat gaagtaaaag gaacaaatgt agacttatac
    42181 ataggacaag gtatatataa gcaaggtcaa tcgagttatg gtggacaaaa tatagcaaaa
    42241 gaaatagtac aacaagtaac attaaataga aagtatagtg aaataaaagg tagtatgtat
    42301 ttctcagcta aagatatagc aaatagtact tctatacaaa aggatttaaa gagtttatat
    42361 tctagtagtg aagaaccagt tactccacca tctaatgtta aagtggagaa attaagaggc
    42421 gacgaaagat atgacacagc agtagcaata agtaaaaaag gttgggctac aaattcagat
    42481 acagtagtat tagtaaatgg gtactctata gttgatggta taacatcaac accacttgct
    42541 acatcaaatg atgctcctat actattggta aataaggata atatacctac atccactaag
    42601 aatgagttaa aaagacttaa tccaagtaaa gttattttaa ttggaggaaa taactctatt
    42661 ggagataaag ttgagagtga gataaaagat acattgtcaa atgtatcaat aaatagagtt
    42721 ggtggttcag atagatatag tacatcttta atgatagcaa aagaattagt aaaaactaat
    42781 ccagtagaaa aactatatat aacaagtggt acaggtgaag cagattctct ttctatagct
    42841 tcaaaagcag gagaagaaaa gcaaccaata gtattagtat caaaagataa tgtaagtgat
    42901 gaagtttaca attggatatc agatttaaaa gtaaaagatg cttattttat aggtggtaat
    42961 ctaagtatat ctgattcagt aataaataag ctggataagg tcattacaaa tgatgtatct
    43021 aaaaatagaa tagctggaga aaatagagaa gaaacaaatg gaaaagtaat acaaaaattc
    43081 tatccaaatg ctgaatattc atcaatgttt gtaagtaaaa gtaatcaatt ggtagatgca
    43141 ttaacatcag gtcctttagc agctaaatta aaatcaccag tagttatgct tggaaatagt
    43201 gtaacatctg ctcagaaaac tgctttagag cctaagaaaa ctactctagt atacgaagct
    43261 ggagatggta taaatcaaaa cacattaaac actttcttaa acttagttaa gtaataaaaa
    43321 agttaattta aaatacagag tattttataa atgtagatta aaatgaaaat tatcatgata
    43381 aattagaaaa taaaaaagaa ctatatgcat tttgtaatca agtgtacaca gcaacttgaa
    43441 aataattatg cgatatagtt cttttttagt aattaatctt atacttttta ataaacttta
    43501 tgttgaattt tttattgttt aaactctatg tcattaaata taaaatcgtg taatatgtct
    43561 aaagtatctg ggtcaaatct aagaacccat ccagcttttc catatatacc acctgtacta
    43621 tgaatttcat catcaatagg aaattcaaat tgcttaatat ttaagtcgcc catttttaaa
    43681 acttgagctc ctaaagaaag tattgcatta ggcttcatgt tagttttaac atatggaaga
    43741 actgcatcta ataaacctgg atatttagta acaggtaaag tttccgtttt cttgattata
    43801 gcttgtataa tctgtctctg tctgccatct ctagcaaagg ctgtatcgtt gtgtctgata
    43861 cgtgcaaaag atagtgcctg atatccattt agagtctgct ttccagagtt tctaatgtat
    43921 tgtatgctac ctttatcatc acttttattc catttataag tttcaggaat aaatttattt
    43981 agttctcgta tttctccttt ttcaacagta acctcaacgc caccaatagc atcaattata
    44041 tacataaatg attcgaaatt aacacaagca taattttgaa tatcaatatt aaaattctct
    44101 tcaattgttt gtattaataa atcagcttgt ccataagcat aagcatgagt aagtttttgt
    44161 ttaccatgac cagggatgtc tacatagcta tcacgagcta atgatgtaag tttgatatca
    44221 ttgtgtttgt tatcaatagt aagtatcatc atagcatctg accttgaaga atcttcattt
    44281 ggtctagcat ctgttcccat aagtagtatg ttaattatcc catcttcatt tttatggtca
    44341 ttgttatcca ataaatcact acttatgctc gagtcatgaa tagcagataa tttataataa
    44401 aaatatccat agacacttat agggaatatt actaccaaga aagccagtag tataacaaat
    44461 ttctttaatt ttgacaaaag taccccttct ttcttctttt tcctgtatat tttttaatag
    44521 catacactta attatattaa agtattgaga tatattcaat acttgtttca gttacaaaaa
    44581 aaagacaatg atataattta taagaaaatt tatcgaccta tatatatagt taaaagtata
    44641 tataatatat tttttaaaag taatttatat tttaaaataa aaacacaccc cataaataca
    44701 aagtaatttg catttaagag gtatgtattt atcatcaaat tattggtttg ttttggaatt
    44761 tctaatcttc aaccataata tctttaaata tgaaatcatg cattgcatct agttcatatt
    44821 cctcaaaagg tattacatag cctgcattac ctatttttct accattttca gtagaaagag
    44881 gaaactccat agatttcaaa tttaaatttc ctatacttaa aagttctttt cctaaagata
    44941 ttatttcatt aggattcata ttagttttta catacggaag tattgtgtta actagattag
    45001 gatatttagt gactggtaaa tccttaacac catttattaa actctgtata acacttcttt
    45061 gtcttctatc tctttcatct gtactatcat tttttcttat acgagaaaat gcaagtgtct
    45121 gatatccatt taatttttgc tcaccagaat ttcttatata tttaagagaa cctttattgt
    45181 catttttatt ccatttataa gtttcaggaa taaatttatt taattctctt atttcactct
    45241 tatcaacagt aacagtaact ccacctaaag catcaactat atccataaaa gagaaaaagt
    45301 caactacggc gtagttttgt atatcaagtt ggaagttttc ttctatagtt tctattaata
    45361 agttagcttg tccatatacg taggcatgtg ttaatttttg ttctccacgt cctggtatat
    45421 taacataagt atctctattt aatgaagtta gcttaagact tttattttta ttatcaatag
    45481 taagtatcat catggcatca gaacgagaaa cttcttctcc tggtcttgca tctgtaccta
    45541 acaataatat gtttgtgata ttttcttcgt ttttatagtc attactacta agcgcctctg
    45601 tatttatact atcatcttgt atagttttta atttagtaaa aacatatcca tatatactaa
    45661 caggaaatgc tataactaaa agggctagaa atataacgaa ctttttgagt cctgacaata
    45721 atatccccta ctttctaaat tttttaatta cacatagtta attatattaa aaatttgtta
    45781 cataagcaat aaaaaacaca ataaattagc ataagtttgt cataatataa ttatttttat
    45841 agcattttat agaagaaagt gtgtagttat tattgatttt attggaatat aattgtatga
    45901 agacttaaat ttttataaac tgatgtgtat attgaaaaaa atattttcct gaatgtagtt
    45961 atagcatctt atgaaaggtg caaattgtaa tttaaaattt ttagtgtata ataagaattg
    46021 aaaattcaaa ccgataaaaa ttaataaaaa ataattataa attaataatt tgggggagga
    46081 aatgtatgat aaaattaata gcaacagatt tagatggaac tttgttagat gaaaagagcg
    46141 aaataaatcc agagttttat aaagtattta aaaaattaag agagagaggg ataatgtttt
    46201 ctgctgctag tggcaggcag tatcaaaatc ttataaaaaa atttgaagat ataaaagatg
    46261 atatgatgtt tatatcagaa aatggaacac tagtagtata taaaggaaaa gaaatattat
    46321 caaatccttt aaataaagaa ttagtaaatg aaatcataga gacaacaaga agtataaaag
    46381 ggaaaaaaat tgtaatgagt gggaaaaaat acgcttatat agaaagcaaa gatgaagcgt
    46441 ttatacaaga ggtcagtact tattatgcaa agtttaaaat tgtagaagat ttgacgaaag
    46501 tagaaggaga catattgaag atagctgtat ttgacttcaa aggtgcagaa cataataata
    46561 atatctactt tgaaaagttt tctgatagag ctcaagtatg tatttctgga gtggagtggc
    46621 ttgatttaac tgcaaaaggt gcaaataaag ggagtgccat aaaaaaagta caaaaaatgt
    46681 tagatataaa atatgaagaa actatggtat ttggtgacca gttaaatgat gtggaaatga
    46741 tgaaaagtgc ctatcatagt tatgccatgg aaaatgcaaa tgaacatttg aaacaaattg
    46801 ctagatttag agcaaaaaga aatactgaaa atggcgttgt agataagata aaagaggtta
    46861 taaaaattgg atagtcatat gtatagatga aaaagattat tttgaagaaa tcaaaaaaat
    46921 atcaatgtaa ttttgataaa taagtttatt tatgcgaaaa ttacattgat atttaataaa
    46981 catcaataat agatatttat ttttttatga tattcagtat tttgttttct atttgagttg
    47041 cattttcagc agaaattaaa taaacatatc tacctgcctt ctcaattata gtattatttg
    47101 ctatttcaga attgattttt tctccatgtc cttctccaaa aggtgcttgt atcatatctt
    47161 ttttataatt ttctagagtt ttatagattt tatctacctc gtcagttttt ataacgataa
    47221 tatcttctag agataatttt tcagaagaat taattacaaa accttgaatt atgtcttcct
    47281 ctacatcatt aaaatagtta aaatcctttg catcaaagat ttttgattcc tttataagta
    47341 aatcagaaga ttttaatgct ttttttattt ttgatgtaga aacgtttttg taatcaccac
    47401 ttgaagaaca actaaccatt cctaatgata agattatcga aaaaaacaat attaaaattt
    47461 ttttcatgat aaacctccaa gttttttaaa ctctgtctta aattagttta ctacataatt
    47521 atataaaaat aaatgtattt aatgttacag ttttgattaa taatgtaata ttttgataat
    47581 aatacctatt gagaggtgat tagaaatgag aataccaaaa catgtaggaa ttataccgga
    47641 tggaaataga aggtgggcaa gtcaaaatgg tatgacaaaa gataaaggat atgaaagtgg
    47701 aatagaccct ggactagagt cttatagatt atgtagagat ttaggctgtg aagaggtgac
    47761 tttttatgga tttacaactg ataatacaag acgtccatca gtgcagacaa aagcttttac
    47821 aaaagcatgt attgatgctg caaatatatt aatagatgaa ggtgctgctt tattagttgt
    47881 aggaaataca aaatctccaa tgtttccaga agaattatta ccatatacta atagaattaa
    47941 agccaaagaa ggtcaaataa aagcaaatct tctggtaaat tatggttggt attgggactt
    48001 aaataatata tcaggagtag aaaatgtaaa taaaaataat atacaggatt acatacagtc
    48061 atttgatgta tccagactag acttgataat cagatggggt ggaagaagga gattaagtgg
    48121 tttccttcct gtacaatcaa tttatgcaga tttttatgtt atagataatt attggccaga
    48181 ttttaagagt gacgatataa aggacgcatt tagttggtat gatacacaag atgtcacttt
    48241 aggtggataa ttaaaaatat tctgatttaa ggttgtagag tgttttaaat tattgaaaaa
    48301 aaaacttctt gatggtaaaa taacactaat aatatgtaaa agtgtaaaaa tttataccaa
    48361 gaagggtgaa gaaatgtcaa aaggcaataa taacaataat tcaagaaaca aatctaaaaa
    48421 gaccagtcat ctgaatagaa aaaagcgaaa attaaataag aaaaagttag cagtgttgat
    48481 ttgctttaca gttttatttt tatttattgc atttaaggct actcaaggtg tagtagcttt
    48541 ggtaaaaagt atggataaaa gtaataaaac taatcaacaa caaaatgtta actcagaaca
    48601 atttgacctt ggtaatgaag aggaaaataa aaagaaaaaa tatactgtat ttatagaccc
    48661 aggtcatgga ggcaatgata aaggaactga aagcaagacc agtaatagat atgaaaaaga
    48721 cctaaatcta caaattgcaa aaaaattagc aaataaatta tctaaacaaa aggatattca
    48781 agtagtagta tccagaactg atgatacata tatatcatta aaggatagag ctatcttagc
    48841 aaataatagt tctgctgatg tgcttgtttc tattcattta aatgcagaga aaaatggtaa
    48901 tacagcaaca ggtatagaaa cttggtatag gaataaagct acagatggtt caaaagaact
    48961 tgcacagaca gtacaaagta ctatagtatc ctatgttaaa gttagagata ggggaatagt
    49021 tgaaaataac tttgaagtac ttagagaatc aaatatgcct gctatattaa tagagtgtgg
    49081 ttttttaaca acaccttcag aggaacaaaa aataataaat gaaaaatatc aagaccaact
    49141 tgcggaagga attgttcaag gtgtactttc atacctagat tctaaaggga ataagtaagt
    49201 taactttact ttaatgtgag gataaattta aaagtttaac tgtgtaaatt gtattaactt
    49261 tatttcgtaa tgttgcaaac attgtttgaa tttagaaact atgaaattag aaattatttt
    49321 atctata
//
LOCUS       DAELNY010000009        47829 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.009, whole genome shotgun sequence.
ACCESSION   DAELNY010000009 DAELNY010000000
VERSION     DAELNY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.009"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..895
                     /locus_tag="KPE55_000440"
     CDS             14..895
                     /locus_tag="KPE55_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG0491542.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1208..1987)
                     /gene="hisJ"
                     /locus_tag="KPE55_000441"
     CDS             complement(1208..1987)
                     /gene="hisJ"
                     /locus_tag="KPE55_000441"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG0491543.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2533..2883)
                     /locus_tag="KPE55_000442"
     CDS             complement(2533..2883)
                     /locus_tag="KPE55_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG0491544.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2899..3315)
                     /locus_tag="KPE55_000443"
     CDS             complement(2899..3315)
                     /locus_tag="KPE55_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG0491545.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3334..3525)
                     /locus_tag="KPE55_000444"
     CDS             complement(3334..3525)
                     /locus_tag="KPE55_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG0491546.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3676..4491)
                     /locus_tag="KPE55_000445"
     CDS             complement(3676..4491)
                     /locus_tag="KPE55_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG0491547.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4538..5476)
                     /locus_tag="KPE55_000446"
     CDS             complement(4538..5476)
                     /locus_tag="KPE55_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0491548.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVNKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5473..6345)
                     /locus_tag="KPE55_000447"
     CDS             complement(5473..6345)
                     /locus_tag="KPE55_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0491549.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6370..8298)
                     /locus_tag="KPE55_000448"
     CDS             complement(6370..8298)
                     /locus_tag="KPE55_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG0491550.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8410..8787)
                     /gene="gcvH"
                     /locus_tag="KPE55_000449"
     CDS             complement(8410..8787)
                     /gene="gcvH"
                     /locus_tag="KPE55_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG0491551.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8874..11000)
                     /gene="cdhC"
                     /locus_tag="KPE55_000450"
     CDS             complement(8874..11000)
                     /gene="cdhC"
                     /locus_tag="KPE55_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG0491552.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11057..11863)
                     /locus_tag="KPE55_000451"
     CDS             complement(11057..11863)
                     /locus_tag="KPE55_000451"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0491553.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11913..13280)
                     /locus_tag="KPE55_000452"
     CDS             complement(11913..13280)
                     /locus_tag="KPE55_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG0491554.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13311..14255)
                     /locus_tag="KPE55_000453"
     CDS             complement(13311..14255)
                     /locus_tag="KPE55_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG0491555.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14275..15045)
                     /locus_tag="KPE55_000454"
     CDS             complement(14275..15045)
                     /locus_tag="KPE55_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0491556.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15101..16486)
                     /gene="lpdA"
                     /locus_tag="KPE55_000455"
     CDS             complement(15101..16486)
                     /gene="lpdA"
                     /locus_tag="KPE55_000455"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0491557.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16530..17411)
                     /locus_tag="KPE55_000456"
     CDS             complement(16530..17411)
                     /locus_tag="KPE55_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG0491558.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17447..18121)
                     /locus_tag="KPE55_000457"
     CDS             complement(17447..18121)
                     /locus_tag="KPE55_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG0491559.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18156..19028)
                     /locus_tag="KPE55_000458"
     CDS             complement(18156..19028)
                     /locus_tag="KPE55_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG0491560.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19120..19752)
                     /locus_tag="KPE55_000459"
     CDS             complement(19120..19752)
                     /locus_tag="KPE55_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG0491561.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19857..21533)
                     /locus_tag="KPE55_000460"
     CDS             complement(19857..21533)
                     /locus_tag="KPE55_000460"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG0491562.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21782..22561)
                     /locus_tag="KPE55_000461"
     CDS             complement(21782..22561)
                     /locus_tag="KPE55_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0491563.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22871..24790)
                     /gene="cooS"
                     /locus_tag="KPE55_000462"
     CDS             complement(22871..24790)
                     /gene="cooS"
                     /locus_tag="KPE55_000462"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0491564.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMTEKAEA"
     gene            complement(25397..26308)
                     /locus_tag="KPE55_000463"
     CDS             complement(25397..26308)
                     /locus_tag="KPE55_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0491565.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26424..27002)
                     /locus_tag="KPE55_000464"
     CDS             complement(26424..27002)
                     /locus_tag="KPE55_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0491566.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIDI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTIVPGVTIGDNVII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            27228..28151
                     /locus_tag="KPE55_000465"
     CDS             27228..28151
                     /locus_tag="KPE55_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0491567.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(28323..29465)
                     /locus_tag="KPE55_000466"
     CDS             complement(28323..29465)
                     /locus_tag="KPE55_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0491568.1"
                     /translation="MDFKIEKYLLKKTEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(29483..31237)
                     /locus_tag="KPE55_000467"
     CDS             complement(29483..31237)
                     /locus_tag="KPE55_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG0491569.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            31373..31579
                     /locus_tag="KPE55_000468"
     CDS             31373..31579
                     /locus_tag="KPE55_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491570.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(31584..32507)
                     /locus_tag="KPE55_000469"
     CDS             complement(31584..32507)
                     /locus_tag="KPE55_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491571.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRIVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(32762..34462)
                     /locus_tag="KPE55_000470"
     CDS             complement(32762..34462)
                     /locus_tag="KPE55_000470"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG0491572.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(34925..35338)
                     /locus_tag="KPE55_000471"
     CDS             complement(34925..35338)
                     /locus_tag="KPE55_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0491573.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(35404..37782)
                     /locus_tag="KPE55_000472"
     CDS             complement(35404..37782)
                     /locus_tag="KPE55_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0491574.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            38101..39552
                     /locus_tag="KPE55_000473"
     CDS             38101..39552
                     /locus_tag="KPE55_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG0491575.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(39756..42269)
                     /locus_tag="KPE55_000474"
     CDS             complement(39756..42269)
                     /locus_tag="KPE55_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0491576.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADTALYDSKRQGRNRFKIV"
     gene            complement(42492..43313)
                     /locus_tag="KPE55_000475"
     CDS             complement(42492..43313)
                     /locus_tag="KPE55_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0491577.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(43832..44308)
                     /locus_tag="KPE55_000476"
     CDS             complement(43832..44308)
                     /locus_tag="KPE55_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG0491578.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(44343..44921)
                     /locus_tag="KPE55_000477"
     CDS             complement(44343..44921)
                     /locus_tag="KPE55_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0491579.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(45025..47625)
                     /locus_tag="KPE55_000478"
     CDS             complement(45025..47625)
                     /locus_tag="KPE55_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0491580.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        14641 a         9150 c         5370 g        18668 t
ORIGIN      
        1 aggagaacta aatatgaaaa aaagattaat aataatgata ttgtctgttg tcttagtact
       61 atcaagtata cttacgatat ttgcatattc aaatattaaa tataataata acaaattgat
      121 atattctaat atgatagata aaaaaactca aaatagtgta aaggaaatcc taaaagaaaa
      181 taaaattaat gaaaaagaca tagatacatt tattaaagca gtcaacaatt ataataaatt
      241 gcaagttaaa atacttcaaa ataatataaa tatatctaaa tctggatatt cttcaatcaa
      301 tgcaaaacaa gttccatata acctagaaaa attgcaagat aattgggtga aaaaattccc
      361 tgattatatg gatgtaaatt gtagaataac tgcttttaga ctttttaaag attttataaa
      421 ttcaaataag aaatttactg gagactctat tgatttgaat gtcgacttgg acactataat
      481 gaataataaa gatgctaaat ttagcaccaa agatgtagaa aaatttatta acttcttttc
      541 tgcaatacca gctaaagaca cagatgatac aatcaaaatt gcagaacaaa taaaaaatga
      601 atggaaaaaa agaaaaatat cttttaaaaa taataaaaat atatctatta taaatggatt
      661 tcttcgttat ccagaaacta aaaacgtatt tataggtcat acaggcatat gtattaaaac
      721 taaaaatggc attctatttt tagaaaaata tggtgttaca tcaccttatc aagttactaa
      781 atttaaaaat aaagaagatg taaaaaatta tatgtttaat agattgaaga tgtcagaagg
      841 tgaaattgaa ttacctgacc ctataataat ggaaaacgat aaacttatga aataataact
      901 ttaaacgcaa aatagtattt ataattacta agtcaatata atagagaagg tctatagatt
      961 tgtgtaaata caagcttatc tacaatctta tataagaatg tttatataaa taagatagat
     1021 taatcctaag tcctattatt ctattgaatt ttattattat tttatttaga ttctattgtt
     1081 attttactta gaacttatta ttattttatt taagttctat tactattttc aatcatataa
     1141 atttacaaaa gactgtacat aaatttattt tatacaaact tatctacagt cttttgtgtg
     1201 aatatatcta aataatatcg tatttattta tttcctctgc acagtgagaa tcagaaccta
     1261 aaactaatgg aatatcgtat ttttttacca actctaataa gtatccatct atataaggct
     1321 ctttgcagta ctcttttcta tttccagaaa cattataatc aagttcataa cctttttctt
     1381 taattaactt tattaactct tcaagaattg tatttccttt ataatcgtat ggaaatacct
     1441 gattaaactt tctaactaaa tttaaatgac ctattctttt tggtttatgt atacctaaat
     1501 ctgaatttac agcttttatc aatgtttcat aatacttatt ataaacagct tcaatacttc
     1561 ctaacttgtc gattaaattt tgaaactcct ctacactgta atctacacaa taataatcat
     1621 catcaatttt aattatatgt actgataaaa gagaatcctc taattcttct ccatatttat
     1681 ttaaattcgc ctttatccct tcttcgtatc cttctataaa atccacttcc gacccaacat
     1741 ttatcttgat tttaccctca tacttttctt tcaacttctt gacatcatta aaatactttg
     1801 gtaaatcttc aatactcata caactatcat tttcaggagc agggtctcta aatccatttg
     1861 catacggaaa atgttctgta aatgtcattt ctgttattcc aacatccatt gctctttgta
     1921 catatttttc aaaatcatcc ttacttccat gaggacaata aggtgaatgt atatgaccat
     1981 ctttcataaa taattcactc cttttttaaa gaaaaaatct aatataaatg cataaatctt
     2041 ttaacttcaa aataatattt ttaatcatgc taatttttat tatatctaat attagagtaa
     2101 ttttaaactg ccttttaagt atcttatcat tagtactatt ttcaacttaa cattaatatt
     2161 attttttaac ttaacattaa tactatcttt aaatagtaat actcaatata taattactta
     2221 acataaaata actcaaattg cttgatgagg cttttgcagt atttaaagaa tatataatta
     2281 tttaacatta aattatccaa tcttgtaata aaatttactc attaaagtat attacagaaa
     2341 atacctcaaa ataaactttt tagttcatga gatactcttt tttacatgaa attagatata
     2401 tttttatcat ttcaacaatg aaagggaggc ttatatccat ctttagacaa ccccttagat
     2461 aaaattttta ataattaagc atcacaaaat ataaaaatcg cttatatcaa acgtataagc
     2521 gattaattat atttacatat gttttttagc ctctaacaac attttattag cttcttccat
     2581 atactctata gccttttcaa tactctttgc tgtttcttct tttccaatag ctctagcctt
     2641 ttcaacccac tctctaaatc cttcttcatg agtttcattg tgatttatcc aatgtactaa
     2701 aagtattttt aaagtttttt catccttaga ctctgatgat tcctcatgac tatgttcgtg
     2761 gtcgtggtcg tgagcatggt catgtggatg ttcatgacat atatctccat gagtatgttc
     2821 gtggcagtgg tcatggtcat gatgtccatg atgatatctt acaatctctt tatcaaaaat
     2881 cattagttat tccccctgtt attttatatt aaactctttt tttataaggt ttacagaatc
     2941 ttttacaagt agtgctaatg gtttaccttc taatccaact aactccacat tttctggcat
     3001 aataggtaaa agtatcttaa gtgcctcact gtctgcaata gcttcactta tattacaagt
     3061 aacttctccc atcatagaat taggtattat tacagaaata ggtgcaacta ttatatttgc
     3121 ttttttagct gatactacta tagcattttc ccctgtagct cctttattag catgagcttt
     3181 catcatagaa ctagttgcta tagaattagt ccctagtgca taaatttcta catatgtagg
     3241 taactcttct ctcaaagaag aaactatctg ggctccaata cctccaccca ttccatctat
     3301 cactgctatt atcatatgaa attcccctat taattaattt tcttgtatta ttatcttatg
     3361 gtccatcaat cttatttctt taattatccc atctattatc ttttgctctc ctaataaatc
     3421 cgttaaataa atctttccat cacaagggtc tatattaacc acattttcca taaccttttc
     3481 taactcatta ttacttttac aaatgaaagc tgatgattca cacatataaa tctcctccta
     3541 atatgatttt tattaaaaat caaaaaaaga ttcccttagg tatttcaata caccaaagaa
     3601 accttcatga tttcagtttt tgtatattat tataccttaa gtatatcact ttaatttatg
     3661 taaagtcaat taaagttaac gctttttatt atactcttca ttcttgtatt tttcatatag
     3721 ttctttaact aattgcttct cataatcagt caaatcgccc tccacaaact caacattaaa
     3781 ttgttcagct aatcccttta ctatgttttt ctgtagaata tctatgtcta tttttctgcc
     3841 aatttcattt tctattccac ttgctttttt agctgtaaat ttcatagctc tttctttcaa
     3901 ttctggtgtc ttagttttta ttaatttaaa caatttttct acatcaaaat ccagtattat
     3961 tgaaccatgt tgtaataaaa caccatcttt tctgctttga gcacttccaa ttaccttctt
     4021 attattaata gtaacttcat atgatgcatg tgcattaaaa catgcagctg ataaattttc
     4081 cctacttatt ctctcgcctc tatttaaatt atctgtttct ataccactta gattcaaccc
     4141 tttaactaga ccttcactta tatatctata agataggtta atgctcttat cgattaaagg
     4201 attatcttct cctataatta tactataggt tagctcatta tcatgtaata cagctcttcc
     4261 acctgtaatt cttctagtat agtctatatt catacatcta cacttatcta aatctatctc
     4321 atcttccaat ttctgaaaat atcctatact caaacaagct ggttcccatg tataaaatct
     4381 aagagttggc ttattatgtc ctttcttata tgcagtaaat atagcttcgt caattgccat
     4441 attcattgct ccttcatatg atttattatg tataactctc cactgattca tgattattac
     4501 gcccccattt ttagtataaa tatatcctag aaatcctcta tagatttatt tctcttattg
     4561 ctaaatctat ctcactttga tttaaaggtc ttgaatagtt gtatatagta cttcctggtc
     4621 tttcattata gtatggtcta ttacaatcct tacaacctct tatttcaaaa ggatagcctt
     4681 tgtttatgtc ttttaaaata tcattgtcaa gctttatact atttaagtat ccatttttaa
     4741 acttaaaata ctcttttgga taacctttat ttatcatata agacattaac tgtacccttc
     4801 tataactttc tatgcttggt tgacttatct tttccatttt agttcctcta actggagtaa
     4861 atgcaaataa actaatcgtt acatcatttt cctttaaata agtatataaa ttgtatatat
     4921 cttcatgact ttcacccata ccaactatta tatgtgtact tattttattg ggatatgatt
     4981 ttgacatttc agttataaat tttaactttt catcatagtt atttcctttt attttttcat
     5041 atagttcttt atttgctgca tctattgcta ttcctatttt atttactcct gctgaaaaaa
     5101 acttctttat ctgttcatca ttttctaatt ttgcagatag tgaaattggc atgtctatct
     5161 taccacttat atagtttata aagtctaaaa cagagttatg tgcttcttca cttgccattg
     5221 attgaataca tattctcttt atattttttc ctttataagc ttttaaagca tctaatattt
     5281 cttcttttga aaactctggc caaattactc ttgataattt atcttttcta gtactacttt
     5341 ctattgattg agaacaaaaa ctgcatttat ttatgcattt ttctccaatc attatatatg
     5401 ctgtggtagg aggtatatcg ctttttttgt ttaatatccc aagttctatt gctgtcccaa
     5461 ctgatagtct aatcataaaa cgcaacactc cttacactca tttattgtca tattcatttc
     5521 tatagcctta ttctttgcag accttgatgg taatactatt ctattgactc cacacattat
     5581 agataattgg tctagctctt ttctatacac tcctcttggt ctcatacaac ctagattaat
     5641 ttcagtatct ggtaaattaa ttctagcttc acataaaata tctgctaccc cttctaactc
     5701 aggaggttct acattttcat actcagtccc ttttgttgga ataaggacta taaatgtaag
     5761 cttattagga ggattttttt gtaggtattc tataatttca tattcaccct ttatttgtcc
     5821 tccttttaaa ccaatacata tatgtggtgc tacttctgta tgttcttgta ttgtattata
     5881 gacttcaata taatcttctt tactcttttt catcttataa acttctctta ttgtctcatc
     5941 atcaaacact aaatcaaaag aaactatatc taaatactta cacaactcta caatgctatc
     6001 tttatccata agacctaaat gagcattcaa tctatatcct tgctctttta agtttttaat
     6061 ggtattaata tgagtattta tagggacatc tccctcataa ctacaaccgc cactcaatag
     6121 aaagctagtt atattccttg attgtacttt ttcctcatat tcttctattg gcaccatatt
     6181 gtttaagtag tgaccgttgc aatgggcaca tttcaaacta cattgattgc ttgtagtact
     6241 aagagctagt gtctggttag gataagcaaa ctctatttta tttccaaaat tctttaattt
     6301 aatatcaaat gctattttca ttttttcaga tagattctta ttcatagttt attccttctt
     6361 aagtacattt tactcttcta gtaatttctt tgctgttggg aactgctcta tattcgtatg
     6421 tggtaagaaa catgcagaaa taaattcatc catataactt ggatatacac taagttcaac
     6481 ataagttatt tgattaccta tttcttcaag tttatttttt gcatctttac ttataagagc
     6541 taagtaagaa ccaacaagtg aactattccc tatataagta aatttttctc tatcgatatc
     6601 aggaagtaac cctataatta ttgagttttc tatgtctagg ttgtttccta taccacctgc
     6661 tatataaacc ctatctaata tactaaaatc catacctaaa ctatctacaa gagtgtatgc
     6721 gcctgaatat atagctcctt tagctctgat gaaattatct atatcaactt cattgactac
     6781 tatatcattt tctaaatcaa attcttcttt aaatgctaat acatactctc ctatctcatg
     6841 ctcattaaat ctaactctct tattatctaa atccctgtat attttacctc ttctatctat
     6901 tacaccttta gtaatcattt gacagattaa atcaattata ccagaaccac atataccaac
     6961 tggtgcacat tcatcaataa tctttaatgt tggctctaaa gtatctttat caataattac
     7021 tttctctatt gctcctgctg atgctctcat tccacagctt atccctccac cttcaaaagc
     7081 aggtcctgct gaacatgcac agctcatcat ataatcttta tttccaaata caatctctcc
     7141 attagttcct aaatctataa ataaaacatt ttcttcactt gaccatatcc ctgctgataa
     7201 tactcctgct gttatatctc ctcctacata acttgcaaca gatggagcta agtacacata
     7261 tgcactgtca tttacaaaaa gaccaacatt ttcacccata agttttggag attttaaaaa
     7321 tggtggtatg taaggttctt gtcttaagaa gtctgtatac acacccaaga ataaacttgt
     7381 catagttgta ttcccagcag ccactaatgt aactacatct tccttatcta ttccatttct
     7441 ttcatatatt gtttttaata gtggatttat agtttcctca actatagctt tatgaagtgt
     7501 ctctaatcca tttttcttag ttgaatatat aattctattt ataacatctg ctccatattt
     7561 tatctgtgca tttccagaag atgctttatc tactacctct tttgagtata ggtctactaa
     7621 acaaacaact actgaagttg taccaatatc tattgcaact ccgtataagg aattttcttt
     7681 atttccttgt tctatattta tgattgtaag tttcttttgt ttttgaacat atgttattgt
     7741 aaccttaaaa tcactctttc taaatacagt tggcattttt ctaagtatat ccaatctaaa
     7801 atctatttca ttatatccta ggttatttct aacatatctt tccaatctat ctacatcact
     7861 tatattatca tctaaatttg gttcttccat ttctatgtat ttctttttta tatttgtttt
     7921 aaattgaagg ttatgttctt caattatttt tttagctctt tcaaaaattt ccctatcctc
     7981 ttttttatta ctaccttcta tcttcatacc atgcatagag gatgatactt tagaaggaac
     8041 ttcaatttct atatctgata ttacctttgt acaacaagct agtatataac cttgctccca
     8101 ctcatcatct gttatatgtc ttgttttttc tgtatcaact tttccattca ataattttac
     8161 tttacacttc ccacaagaca cgtttccatt acaaggagca tctatgaata tatctgcatt
     8221 tcttgcaacc tctagtaata tatccccctc attacaataa acctctttat tgtttggtgt
     8281 aaaacttact tttatcatgt tatccctccc aatttgttct atatatgata aaaggaaagt
     8341 atagctaaat ttgcgtgcac tcaaatttta ctatactttc cccaattata ataatatctt
     8401 aatctttaat tattctaatc cagcctcgta ttctttatca cttaataacg tatcaagttc
     8461 agccacatca gctagtttta cttttaatat ccagttttca tatgggtctt catttataca
     8521 ttctggctca tcttctaatt tttcattaac ttccaatacc tcaccattaa caggagatat
     8581 taaatcagaa gctactttag aagactcaac tactccaaaa tctactccct tagttacagt
     8641 atcttctact tctggtgttt caacaaacaa tatttctcct aattgatctt gagcatagtc
     8701 tgttatacct atatatacaa catctccatc aattactttt acccactcat gatctttaga
     8761 gtattttaat tctggtaata atttcatttc gatagacccc caatattata ttattgatta
     8821 aattacttaa atcatattaa tattatatca tattatttta aattttccat caattacatt
     8881 acactttcca tagctaatgc tggatgacct ttttcttcta agaaagctaa tactccttca
     8941 gaatcgtcag ctatagtttc atcacaaacc atatcacaga agttttctat tccagtcatt
     9001 tctttaactg tagcatttaa tttatcagct acataatctt ttaattcttt tggcatccaa
     9061 actattcttt ctggtccacc ttctgcataa gcgaattttt tagaagatat gaaatgtctt
     9121 ccgtgtccca taaatcctgg agtttgaact cctccacctg tcatagaagc aagttctccg
     9181 aaagtcatac caactggagt aacacttgca aattctctgt taactacaac aaatccattt
     9241 gcttctggca ttataccaca gatacattcg aagcatccac aagaagtcat tgggtcttct
     9301 agtatagagt ataatgtaac agattcaact gcaccttgag atatttggtt tacagtttca
     9361 tttactgaat tccatacacc tgttctatca tctaagcact cgcctttttc tattggttga
     9421 caaggacctg ttgggtctaa ttctttagta gccttagcat ctaaccagct aactgctcca
     9481 caaagaccaa gtctttcagg agtaacaaca caaacgtgag ctggtgcaaa tgattgacat
     9541 aagttacaag aatagaaagt gtcaacactt tcatcaacta atgaagctaa tctttcatct
     9601 ctagcattgt acttagctat tgcttcacct tttaattcag aaactttttc agcatctgta
     9661 attatagtta tttcacactt atcaactact gattcaaatt catctaacat cttagcatat
     9721 aaaacttctc ctatatgctc aagtctaaat cctttatcaa atgcatcttt agagattctt
     9781 acccaagcca tatctctttg tccaacgtgc attacacctt ctatatagtt taagaagtag
     9841 tggaaacgtc tttcaagaac tggctcgaaa tcttcttgca tatttttacc agctatttta
     9901 actattacag caagtggtaa tcttaataca ccatctgcat caacttcatc tatatttggt
     9961 cctattattt ctatcttatg gtcttcaact tctgttgctt ctttcttaac aacaagctcc
    10021 caagcttcag ttctgtttcc accaaactca gcaaacatat cgctctttct aattctctca
    10081 ccttcaaatg ctgctgcaaa tgaaactggg ataggtattt cagtaacttt tatttttatt
    10141 cctctagctt ctaatgaagt agctactatc ttatcgtaat ctttttgagt taataggttc
    10201 attggaactt caagaactgt ttggtcagtt ataactggga atcctaaagc tattgctcca
    10261 gcacctgcag atacaactaa ttcacttaat ggtccaaatg cattaacaaa tgcaggaact
    10321 ctattagctg tgtactctaa taatccgttt aaatcaccag gagttaatcc accgaatatt
    10381 aatgctgctc ttactgcaac agatacaacg tgtataacag aagttacatc atatcctaga
    10441 ggtataactc ttagctcaag acccattttt actccagctt ctatagcttg gtctataact
    10501 ttaccaacta agaaagttag taaaccttta gattggtaat cttttataac ttttgctgct
    10561 gattctgcat ctggacactc accaagaaca actgcaacac caggtatatc tcctgtaact
    10621 aatggtacac caagtgatct gattataggg tcagttatat gacctgcaca tggctcacta
    10681 tatggagcat ccatagttga atattttaat gcttcaatta cttctgctgc taaagctgta
    10741 gctaaaccag cattaaaagc atgttctagt aaatgagttc tatttattaa agattttact
    10801 acttctaaag caccttctaa gtcccctaaa gtgttcatct tttgtcctgt tgcagcatat
    10861 atacaaggta atgaatatgc tgtatcaggg aaagcaactt tatgctcttt tccattcttt
    10921 tctatagctt cagctaacat agcttgagcc gcacctaaag cttgttctga ccctgtaaag
    10981 attatattat atagattcat tctatttccc cctttaatat ttacacctat tttttattaa
    11041 ctatacccta aaaaccctag aaagcaaatc caccttcatt taactctaag aaagagtctg
    11101 catataaaga tgccccgttg attatgtcac aagattttac agttttcatt aattctgggt
    11161 cacatggatt cattatagct gaactaaatc cattagccat tgccattgct aagaatgcac
    11221 tatctaaaac aggtctaaca tgcttaggac atccattaga tacgttagat aatcctcctg
    11281 tagttaaaag tcccatctca gtcatcattt ttatagcttc taaaacttct acttgttttt
    11341 cttgcatacc ttttataact aagcataatg ggtcaaatag tatatcagtt ggctccattc
    11401 ctaacattaa gcctttttct aatatttctt ggcagtaagc catacgctca tcattatctc
    11461 ttgggatacc ttcttttgca cataaaccta taaccattgc ttcgtattca gctgctatat
    11521 ctattaaatc aaatcttgag ccagcatcag cagagtttat tattggtttt gcattagttc
    11581 tatcataaac tttaagtcca gcttcgatag cttttttatt agctgtatct aatgctatag
    11641 gaacattgtt gaactcagat tgaagtaatt tgattcccca tgtcattatt tcttcgccat
    11701 ctctttcggc aggtcctata ttaaaatcta tatagtgtgc tcctgcttct aattgctctt
    11761 ttgctctttt taagattgga gctgggtctc tttctgctaa tgcttttctt attgaaggtg
    11821 atatacaatg tattctctca cctataatca taaatttttc catttataat gtcccccttt
    11881 aaatttaata ttaatagtta gttaatattt tattagtttg tttggcatgc tttttcttgg
    11941 tatcctttta agaacttagg taattccata gattcctctg gtcctataac tacattccat
    12001 cctggtaagt tatcttctat atcaccttta agtactgcaa cttttcctgg aatgataagg
    12061 tctttacaat tagttacttc ttctacctta ctttctttaa tgaaagcaga gattgagttt
    12121 ccaccaaatt taccagcagc ccaagatgta agaactgaat atccacctgc atctggtatt
    12181 actaaccata ctggaacttt tgatctttct atatcaccag caactatgaa gtaagttaat
    12241 gcaaagtcaa cagtaactaa tactggagaa ttttcatctg ggttgttgat tggataaatc
    12301 tttggttcaa ctctcattgg tctttgtggg tcagtgtata tgttttgtct tagtgcaaat
    12361 aatggtaatg ccttagcata actaatatca tctattacta ttatagaacc gtattttata
    12421 gtgaatattg atgataaagc aacttccatc ataggatttg aattagataa tctatttgca
    12481 aatactattg aaggatatcc aaatgttcta tcttgctctt ttaaagctgt tcttctaact
    12541 tgtatagcat tagtgtaagt atcttttatg ttttctcctg ttacatctaa tactaattct
    12601 ttatatccag cagcttgaat taattcaact gtttcatata attcttctaa gctaccagct
    12661 tttacaccta atggtaaact tgctccttta actacttcta tcatatcttt gtagttctct
    12721 ttagttgctc catataccat tggtctagca tcttttaata cttcaacagc ttctttaact
    12781 acttgtgcat catcacaagc taaaatataa gctacttcta atccactgcc ttttatttta
    12841 tctattaatt ttaaatacgc atctttatct ccacaatact ctaaaacagc catttcaact
    12901 ttcatttgtt caccaattct tacatagtca acttttttca tgttagctat tttagcatct
    12961 actgcttcat cactcataca agtacagaat gaaactgcat atctatttct acttactaat
    13021 gttttttcat gtctaaataa tactgtttct ccacctagtt catactcaga tgcaccagca
    13081 ccaactttta aagctttcat taaaggtgca gtagcctctg ataattttgc tatagcgtca
    13141 tcagacatat gtgggcattt acctacttct acagctcctg aagcaacctt catagagaaa
    13201 gccatacaag ttgggaatcc acaatcctta cagtttttct ttggtgttaa tttaaatata
    13261 tctaaagctt ttaatgccat tattttttcc ccctaacctt aacaatttaa ttaagctaat
    13321 gcgttaacca attcttttat agtttctatt gattttggat gacgaagtat aactgcgttt
    13381 gaaccaccta ctaaaacact tgctgcagtt gaaacttcca ttgctatagt tctttcttct
    13441 ggacaacccc aatctggctc atcttctatt ggagctatag cttctttaac atgacctact
    13501 tcaaaagcta ctggtgttat gataggcatt tgtaatgtct tatcattttg accaaatgct
    13561 gcaagtctaa ttctatccat agtagatgca acgtactcat atccataacc aactgctgaa
    13621 catccaacat tcataactat attttcacct ttaacgccta gttgagttaa caatacgttt
    13681 aattgtttag ctaggtttat atcaacagaa gattcagctc ctactttatg tgcataagcc
    13741 ataccagctg atgctcctac tcctttgtag ttgtcttcta ctgctgacat aaataagcag
    13801 ttatgtccat ccaatgtttg agctaatttt tcaaataatt taccatcttt ttcatgattt
    13861 cctgaaccag ctacaactaa aggtaatttt attgcttcaa ttactgcctt agctacatca
    13921 gcacattcat caactgactt atctaatcca tttgggtcag aaccatcaaa ttttaaacaa
    13981 ataaaatctg cttgtgtatt agcttcaaca tatttagccc actctactgg acaattagct
    14041 acatctttgt ataattcctt atatgaatca gtccagcttt caggataaac gtctgatatt
    14101 tgtataccta tttttggaga attacctact tctccatcaa aactataaaa aggtaataca
    14161 ttttccccac ctaatttaat tgctttttcc cctattccta cttcaacttc tgatattttt
    14221 ccagaatatt tttgagtaga cattttaaat gccatatttt tttccccctt aagcctattt
    14281 taattctaat ttagcaaaaa tttctttcat agccacttta gattttgaat cttcaggtaa
    14341 attaactaaa ggaatacctg tagaatcata ttcatatatc aattcatcca taggtacaac
    14401 accgattagc tctaggttat gcttttcaat ttcttcttta acaccatcat tcataacccc
    14461 atttggaact ttatttacaa tcaaaagtat tcttccaaca cttaacttta gttcctcagc
    14521 taaatctctt attctagcaa cagcttgtat gctacgtctt gagcaatcac taaccaataa
    14581 aagtgtatca acatgtctag tgacttttct acttagatgt tccataccag cttcgttgtc
    14641 catgactaag tatttgtaat gaccagatat tttgtttact tgttctctaa gtatcccatt
    14701 tacaaaacag taacatcctt caccttctga cctacccatt actaataagt catatccttc
    14761 gccttcttcg attatagaat ttaatctaaa ttgaagatat tgtgcctttg tcataccacc
    14821 agggaatgca ttgcctaact tttctctttg atttacttct tctcttattt ctcctattgt
    14881 tgcttcaact tcgataccta atacttcatt tatgttagca ttggcatcag catctacaac
    14941 taatactggt ccctttttgt ccttaaccaa ataatcaatc aaaagccctg tgagacttgt
    15001 tttaccagtt ccaccttttc ctgcaacagc tatattgtat cccattttaa gacttccccc
    15061 ttaaatctag ccacagagcc ttatgtactc tgtctgtcta ttataattta ggtacagaat
    15121 gaacacattc tccatgtaca tcatgaagtg cttccattag cccttcagat aaacttggat
    15181 gtggatgtat tgcatcccct acttgttcta ctgttaatcc tagatgaaca gcaagtgaca
    15241 actctgtcaa taaatctgta gcatgaggtc ctactactgc tgcacctatt atcttatcag
    15301 tttctttgtc agctataact tttacaaatc cttggaaatg tcctatagct tgtgcttttc
    15361 caagccctct aaaatcaaat tgacctacat tgtattctac accttcagct tcaagttgct
    15421 tctcagtctt accaacacca gcaacttcag gttcagtata aacacatctt ggaattgctc
    15481 tataatctac taccttagtc ttacctaaag cattttcaac tgctacgata ccttctttag
    15541 aagcaacatg tgctaggaaa ggagtatcta ttatatcgcc tattgcgtat ataccttcta
    15601 cattagtttc aagatgttca ttaactacta cttttcctct ttccatttca attcctatat
    15661 cttctactcc agaattgtca aggttaggtc ttctaccaac acatactaat gcatattgtg
    15721 cttctataac ctttccatta gaaagagttg caacagcctt accatcaact acttcacaag
    15781 tttgtactcc aatacctgta ataaccttaa ttttatcttt cttaaattgt ctaagtagtt
    15841 gtttagctac atctttgtct tcatttaata gtatttggtc aaccatctca actatagtta
    15901 cttctgttcc taaggctcta aagaactgcc ctatctcaca tcctataact ccaccaccta
    15961 ctattaacat agactcaggt atctcttcaa gtccaagaac ttcatcacta gttattacaa
    16021 cttttccatc atatgggaac attcttggta caactggaac agaaccattt gctagtatta
    16081 tcttatctgc ttttatagtt tctactgttc catcatcttt agttacttct atagtgtttt
    16141 tatcaattaa ttttccaaaa ccattaacta ggtttactcc tcttttttca aataagaact
    16201 ctataccact tattaattgg tttacaactt tattttttct ttccattatg gcagtgaagt
    16261 ttggcttaac tgtaccatct atttctatac caaaatcctt tgcttctttt acagtattca
    16321 atactcctga agaagcaaga agtgctttag ttggtataca tcctgcattt aagcaagttc
    16381 ctccaactct tcttttttca acaactgtta catctgcacc aagcatagaa gcttttatag
    16441 ctgctacata tcctcctggt ccaccaccta ctactactat cttcatcttt aattccccca
    16501 aacctaatat aatctacaat cttgcaatac tataaacctg cggcatccag tatagcaggt
    16561 atattctctt ctgccccaat tgccattata tgaacaccat cacataaatc ttcttctttt
    16621 aattgtttga taagttcccc tgccattttt attccttcac ttacccagtt ttcttttcca
    16681 gctgctctta atctttcgat ttgttcatct ggaacaaata ttcctggtac atttgcagtc
    16741 ataaatttag ccattcctgg tgattttaaa ggtactattc ctgctaatac cttgcaatcc
    16801 atatcccttg ttaattctct aaactttctc attgtattta tgtcataaag tgcttgtgtt
    16861 tggaaaaatt tagcccctgc ttttattttc tttttcattt ttaataattg aacttctatt
    16921 ggagaatatt ctggagttac tgatgctcct agataaaaat ctggagaacc ttttaactta
    16981 ttaccagcca tgtccgtacc tgccattaaa gtctcagcag tttgaagtat ccccacagaa
    17041 tctaaatcaa acactccctt agcttgtgga tggtctccta cgctagtatg gtctcctgtt
    17101 agtgccaaca gattgtttat cccaaataca cctgctgaca acatttctcc ttgtatagct
    17161 attctatttc tatctctccc tgttatttga ataactggct ctaatccagc atcttttaat
    17221 aatttacatg tagcaagaga agttgctctc ataactgccg attgaaagtc tgttacattt
    17281 gctgcatgaa ctcttccaac taacggcttt gcacattcta ttaaatgtga aaggtcagtc
    17341 ccttttggag gagccatttc agtagtaact gcaaattttc cactttctaa tgtttctctt
    17401 aataagctca tctctaatcc cccttttaac cttctaaaaa aaacttttat gaatttgctg
    17461 ctgcttcttt ctttttcttt gcacttaaac ttcttggatt attttgctta gaatagtctt
    17521 ttggtggtct tatttctgct aagttatcta attgtcctat agcttctagt ctttcatata
    17581 tctttatcca agcacaatca ttctctgagt ttacttcaca cttaccattt ttagctccac
    17641 cacaagcccc attcataaga cctttagcac acatagttac tggacatatc ccacctgtcc
    17701 aaccaagttc acagtctcca caagccttac aggcttcttc atattctcca actctttgta
    17761 cttcccctat aaacaaagta ttgttggctg gataaactgg cttatctttt aaattcttaa
    17821 ctattgtctg tgtaccatct ccacatgcta aagataaaac tgcatcagct tccttagtct
    17881 cttcttttag agcctttaaa tcctttttag actttaaaag gttacaagca gggtcaagca
    17941 ttacatatcc taagattttt ttgccttcac cttcaagagt ttccttcatt tttaaaactt
    18001 cttcttcccc accactttta caagttgctg cacattgatt acaaccaact aaaaccagct
    18061 tgtcaaaatc ctttaagtac cctagcactt cttctaatgg tttattttca gaaattatca
    18121 tatctacttc ccccctaaac cttccacata tatatctatt tattgttttg taatttacaa
    18181 gccttaacta catgttttgc aagtatagat gttgtaactg aaccaacccc cgctggaact
    18241 ggagttatca ttgatacttt gtctaaaact gcatttgtat caacatctcc acataaattt
    18301 ccttcttcat caacatttat accaacgtct ataactaccg ctccatcttt aacaaaactt
    18361 tcatcaacca tcttagctct accaatagct gctattagca cgtctgcctc tgctgctaca
    18421 cctgatagat ttttagtttt tgaatgacat attgtaacag ttgcatgttc acttaataaa
    18481 agcattgata ctggctttcc aactaccatt gatcttccaa gaactgttac cttacttcct
    18541 tttaaatcaa cattatagtg ttttaatatc tcaactactg ctgtaggtgt acatggtgga
    18601 aatccacttt tatccccttc cattacctta gcagagttta ttggactaaa acaatctacg
    18661 tctttttccg gtgcaataac atattttata acttcttcat ttaagtgttt tggaagaggt
    18721 ctaaaacata atataccatt aacatttttg tcatcattaa ctctctttaa tacatcaata
    18781 tattcctctt gtgttatatc ctcagctaac tctaacactt ctgttgttat acctatgttt
    18841 tgacatctct ttagtgctcc tctttcataa gataaatcat cacttcttgc cccaactcta
    18901 actattgtta gtttaggatt tataccttct ttaactagta aatcgacttc ttttattaat
    18961 tcttcactaa tcttatcagc tacaggtttt ccttttataa tttgtccttt agttgacatt
    19021 ccttccatac attcacaact cccctacaag caaacttaaa ttaatttatt ctatatacca
    19081 catattaata acatagtatc tactttcgtc atttaaaaat tattttccaa gagctttttc
    19141 aactttggca taaacttcat cacaaatttt tacaccatct tcaactaact tatctacttc
    19201 tgtttttacc ttattaacat attctttatc ttgtatagaa tttatattta taactacatt
    19261 taattgtcca ccaagtatag cagctcttaa acactgaact cctacaccaa catcacttat
    19321 agcaagtctt gaacctttat caactaaatc ttcatgtaac ttaatagctt catagcaaac
    19381 tcttactatg tttaatggta cttcacaagc aacttttaat gccttttcca ttgtttctgc
    19441 ttttattctt ttttcttctt ctgtttcttt aggaagcccg taagcttttg ataatggtaa
    19501 gaaacattct gcatcatcat ctatcatttt taataaatct ttttctaact ttcctgcctt
    19561 acttaatatc tcttttacac tttcttcata ctcagcatac ttcttcttcc ctattgtaag
    19621 gttacatacc atactaccta aagccatacc tattgcacct actagtgcag cagctccccc
    19681 accacctggt actgcttctt tggaagatag tacttctaca aaatcaacac aagttttatc
    19741 tgctattttc atgataatcc tccttgaaag gatgtaggaa aatatctcaa attgttcgag
    19801 atattttacg aactatttta agatatctcc tactctttat ttataaatta atttccttag
    19861 aataatccag ctattactcc attttcatca acatctattt tttctgctgc tggaactttt
    19921 ggtaatcctg gcatcttcat tatttctcca gtaagtgcaa ctataaatcc agctcctgct
    19981 gaaattgttg cttgtcttac agttattcta aatcctgttg gtctacctaa tttagtttgg
    20041 tcatcagtta aagaatattg agtcttagcc atacatactg gaactttagt aaatccaagt
    20101 ttttctaagt tagctatttc cttatcagct tcaggagtat aatctacacc atctgctcca
    20161 taaatcttag tagctatagc atttatcttg tcttttatag ataaatcatc ttcatagcag
    20221 aatctaaagt tattttcttc ttcatccaat aatcttaaaa cttctttagc tactgcaatt
    20281 ccaccttctc caccttttgc ccatacttca gaaagagcaa cattaacacc taattcctta
    20341 cacttgtctt caacaagttt aagttcagct tcagtatcaa gtgggaatct attgattgca
    20401 acaactgctg gtaatccata tacttgagtt atgttttcaa catgttttaa taagtttgga
    20461 agtccttttt caagagcttc taggttttcg ttatttaagt ctgctttagc aactccacca
    20521 ttgtatttta atgctcttac agtagcaact attataacag catctggttt taagtttgcc
    20581 attctacatt ttatatctaa gaatttttct gctccaaggt cagcaccgaa accagcctca
    20641 gttattacat aatctgcaaa gtgcatagcc attcttgtag ctataacaga gttacatcca
    20701 tgagctatat ttgcgaatgg tccaccgtgt acaaatgatg gtgttccttc aagagtttga
    20761 actaggtttg gttttaaagc atcttttaat aatgcagcca tagcaccatt agcttttaat
    20821 tgtctagctg ttacaggttc accttcgtag ctgtatccaa ctattatgtt tcctaatctt
    20881 tcttttaaat cagatatatc acttgccaaa cagaatgctg ccattatttc agaagcaaca
    20941 gttatatcga atccatcttg tctaactgct ccatcacctt ttttacccat accatcaacg
    21001 atatttctaa gttgtctatc gttcatgtct acacatcttc tccaagttat tttcttaggg
    21061 tctattctaa gtgcatttcc ttggtgaata tggttatcaa gcatagcagc taaaagattg
    21121 ttagcagctc ctatagcgtg aaaatctcct gtgaagtgta agtttatatc ttccatagga
    21181 acaacttgtg catatcctcc accagctgct ccacctttaa caccaaatac tggtcctaga
    21241 gatggttctc ttaaagcaac taatacattt ttgtctagtt tagcaaatgc atcagcaaca
    21301 cctatagtag tagtagtttt tccttcacct gctggagttg ggtttatagc tgtagttagt
    21361 attaacttag cttttttacc agtctctctt tttagtagat tgtagtcaac ttttgctttg
    21421 tatttaccat aaagttctac atcatcttca cctagtccta atttttttgc aacctctcta
    21481 atatcttgag gcttagcttc ttgagctatt tcaatgtcag atttaaatcc cataactgaa
    21541 tctcctccca caacctaaat agatttaaaa tataattttt gtaaaaattg tattaatatg
    21601 taaaaattat attttataag ataattatac atgtaaaatt acttaaaaac tagctactaa
    21661 gctattaaaa tattcaaact taatagctag ttatttatct aattacttac ttattaccta
    21721 tctattgcct atgtctaacc tttaaccaac ttaaaatccc aaatcaaaat ttctatctat
    21781 tttatttatc cttaagtgac attaatttat cttttattgc tttaatctgt tctttcgttg
    21841 tttcactaga atcatatgga gattgattag ctctatcaga atcaataaca tcttggttat
    21901 aataaataaa tcctaaagat tctccatctt ctaagttttg gattataaat tcttcatctt
    21961 ccttatttct aattttattt cctactacaa atactttatt tacacctata tcatgtccaa
    22021 gtttcttaac ttttctataa gtctgtagac ttctctctcc tggttctaca actactataa
    22081 aagcatctac accttgggct gttcctcttc ctaagtgttc aattcccgct tccatatcca
    22141 taaccactac atctttattt tgtagtatta aatgtgagca gagtctcttc aaaagaacat
    22201 gctctggaca tacacatcca gtaccaccag aatctacggt tcccaatgta agtaatctta
    22261 ctccattgta ttctttacag aaattttcag ggatatcgtc tactttagga ttcatcttaa
    22321 acatttttcc aaatgaacct acagaagcag ctgttctatc tgaaactaat tttttcattt
    22381 ctgatatagg tactattgat tcatatactt cttttggaaa acctagtgct aaagctaaat
    22441 tagcatcagg gtcggcatca acagcaacaa ctctgtatcc gtcttcagca aacatcctag
    22501 agagcattga agaaaaagtc gttttaccta ctccaccttt acctgttatt gctatcttca
    22561 taatccctac caccttttat tactatttgc tttttagtag ttaattttac agttaaatat
    22621 tttttatact ctatattttt atacactaaa aataaactac agctagtcct tacaggaacc
    22681 aattaaattt accctactag cttttcaaag cagcatgtat agttttatta ttctttaata
    22741 ttgtttatat tttatttatt cagtatattc attatatttt gatgttcttt actttaatat
    22801 ttgactttat tcaactttat tcaaccagaa acttcttata aaaacgttta ttctataact
    22861 tcactatttt ttaagcttca gccttttctg tcattttttc ttcaactaat gcttcaaagt
    22921 gaacacgttt tttctctata tcttctatta ttgtagctgc taactctttt ggatttgtat
    22981 ttacagtaaa tgtagctcca actttatctt ttaattcact agttaatata tccactgctc
    23041 ttgaagaacc agctattgga ggcattattc ctaaataagt gtctatacca gaagctacaa
    23101 catagcatcc tatagcaact gctttttctg acatccactc tggagctata cctacaactg
    23161 gaaggtcaca catatccata tctaagtaat tagctgctgc tccaactaaa tctagtatac
    23221 ggcttatatc aacacaagaa cccatatgta gtactggtgg gattcctact aatttacaga
    23281 cagttgcaag acctttacct gcatattttt cagccgcatc agattccatt aaaccaaact
    23341 tagctgctgc tgaagctcca caacctgtag taactactat tatgtcgttt ttgattaatt
    23401 ctttcattat tgttacgtga gcctcatttg aaactccttt agcattgtta catccaacta
    23461 caccagctgc tcctcttaat actccagctt ttagacagtc tgttaatggc ttaactgttc
    23521 ctgctgggtc tatatgagag tttacaactc tgtctaattg atttactact gcttctacac
    23581 tatacccaac tgttgcccct gattttaatt caggtataag tacttttgat ttatctctat
    23641 ttttaaagtt taaaatagct tctttaacta ttgctttagc atcatcaagt gcttgttctt
    23701 ctctaaattc aatataagtt gaacctgtta tcttagcttt tggagaagtt gttacaaatt
    23761 tagtatggta acaatctgca actctagcta atgcagggaa tatacattga acgtcaacta
    23821 ttacagcttc aactgctcca gttattattg caagttcttg ttgatggaag tctcctgcta
    23881 tttttacacc atgtctcatt gtaatctcat tacctgtaca acacatacct gctaagttta
    23941 ttccatcagc accaacttct ttagctaaag ctactaagtc tggctcttct gatgctaaaa
    24001 ctatcatttc tgataatgct ggctcatgtc catgtaatat tatgttaact ttattttctt
    24061 ctaatactgc taaattcgcc tctgttcttc ttggcactgg agttccaaat aagatatcgc
    24121 ttagacgagt acctatcata gaaccacccc aaccatcagc catagaagtt ctaagactca
    24181 tgtgtattaa actatctata tcaccagtac atcctatatg agtagagtgc attattgttg
    24241 caatttctct atctatagct cttggctcta tagcgtattc tttccatatt ttttgtcttg
    24301 gttctggtgc attttttata aatcttaatg tcccaaaagg ttttccaaat tccattaatg
    24361 caatttctgc cacttcatgt gctacatcat agatatctct accttctgtc tctacatccc
    24421 attctttagc taaagtaatt aacttagctt cgtctcttat tttatagttt ccatttggat
    24481 cagctaatgc taatgtatgt gctatatctc tagcatggtc tgaatgggca gctgtcccac
    24541 cagcaaccat tcttgcaaag tttctaccta ctatagtatg ctcatctgct ccacataacc
    24601 cccttggagt cttagggctt attctacatg gtcccattcc acatattcta cagcaaacac
    24661 cttgaagacc aaaaccacat tgtgccttaa agccttcttt tctatcatac attgtttcta
    24721 cgccttcttc tctagcttta gctatcatct tctggctgtt taaatcaatt gttaacattt
    24781 tttcatccat attcctatcc cctttttttg atttttaaaa tcccccaggt gactgacttc
    24841 atgtcataaa ataataaaat aaatgctttc taagttgatt atatactaat tttcagtgct
    24901 ttgctttaga tttaaatatt tttttaacaa aattagataa tacataaaaa ttttattttc
    24961 tacacttaat ttaagttgat tttatcgctt gtttttggct atttgttaat atctaaacct
    25021 acttttttga tacttcagtt taattaaatt atgttttgat ttttttaaat caaatttgaa
    25081 atttttctag tcatttttat attattgcag attgtttgtg tatttattat ctttcactac
    25141 tgtttggact tattacagat ttttactccc taaacttaat tagtaaaaac ttcttagaat
    25201 agcatttttt ggactaattt ttttgaattt ttttattttt cctactttaa tattaattat
    25261 agcttcaaat tgaacatttg tatatattca tttgaagcta taattatttt ttatataaaa
    25321 cttttttaat acagcattaa tttagacatt ttgactattt tttctacttt aattcattta
    25381 tttcaagcga tttatcctat ttcatatttt tagctacaat agccccatat gcaatagaat
    25441 ataatttaga ttcatgtgaa tcagctcttg acactaaaac tataggagct tttgcaccca
    25501 taattatacc tgctgattta gagtctgcca tatatgtaaa ggctttccct attccattac
    25561 caacctctat tgttggaacc aatatgatat ctacatctcc agatacttca cttttaaacc
    25621 cttttatctc actagcttct tttgaaacag ctaaatcaaa agccagagga ccttctacaa
    25681 ttacattttc accaaattct ccttctttac aagcttttgc taacatatct gcatctacag
    25741 ttgcttgcat cttaggattt actttctctt tagctgccaa acatgcaact tttacagttt
    25801 ccattcctaa agcttttgca gcttttatag aattttttaa tatctttgcc ttttgttcat
    25861 aatcaggagc tatattcata cctccatctg tagttataag taatttatgg tatttttcta
    25921 actcatatac cataacatga cttaataaac tatcagttct aagaccatag tctttattta
    25981 acacttcttt taatatgaca gatgtatcta ataatccttt cattacaaag tcagcttctt
    26041 tacttgatac taatttaact gctatttctg cacactcttt gatatcatcc ttatccacta
    26101 ttttgatacc acttaaatca aatcctattt cttttgatat ttctcttatt ttgccttcat
    26161 ttccaaccaa tataggagta attatatttc tttctacagc atcctttatt gccattaaaa
    26221 cttctttatc atgagctgct gctacagaga gtacaatttt ctctcctccc ttaagttgtt
    26281 ctaaaatatc atctaatttt tttatcaaaa gtaacccctc ccttatatat atttaactaa
    26341 ataataccat atttgctata ccttttctca atttattttt tatgttataa agaatttata
    26401 taactaaaag acagtttaat attttattct gggatttcat caataactct taaaagcttt
    26461 gcaggaacac cacctacaat agtattttct gcaacatttt ttgttacaac tgctccagca
    26521 gcgataatta cattatctcc aatagtaact cctggaacta tagttacatt agcaccaatc
    26581 catacatttt ttcctattgt gattggagaa ggataagttg taccacgttt ctctggtgca
    26641 ataccatggt taagtgtagc caataccaca ttatgcccaa tttgcgtatt atctccaata
    26701 tcaatccccc cctggtcttg aaaatggcaa ctgctattta taaaaacatt ttttcctatt
    26761 gtgatgtttt ttccacaatc tgtataaaat ggtggaaaca taaaaaatga ttcatctacc
    26821 ttttttccaa ttaactcaga aaataaacta cggacttctt ctggttcatg ataagaagaa
    26881 tttaacttac ttgtgatttt cattgcttca ttactcaact taaccataaa ttcatgtatt
    26941 tctgatttac tagaaacagg ttttctttga ttgatatgtg ttaaaaattc attgatattc
    27001 attttataca tcctttcaat atgtgcttta atttatttgt acattctaaa taatatttta
    27061 ataaaaatta atttaattta tttttattaa aatatcattg cttcttttag tgtaatatac
    27121 aattatatct atatcaaata tctatattaa atacttaact atacttaaaa agcatataaa
    27181 ttgatataat tatataatag ataagttaat attgaggagg gataattatg gaaataagag
    27241 tattaagata ttttttagca attgcaagag aagaaagtat atctggtgcg gcaacctttt
    27301 tacatataac acaaccaact ctttctagac aaattatgga acttgaagaa gagttgggag
    27361 ttaaattatt ggttcgagga aatagaaaaa tcaccttaac aaatgaaggt aggcttttac
    27421 ataaaagagc agaagaaatt atatcattag ttgataaggt tgaattagaa tttgacaatc
    27481 ccaatgagat tattagcggt gatatatata taggaagtgg tgaaactgaa gctatgcgac
    27541 ttatagctag aactgcccat cattttcatc aaaaataccc caatgtaaga tatcaccttt
    27601 ttagtgggaa tgctgatgat gtgactgaaa aattagacaa gggactgctt gattttggta
    27661 ttttaattga acctgcaaat atacagaagt atgattatgc acaaattcct gctactgatg
    27721 tatgggggtt actaatgcgt aaagatagtg cccttgcatc acaaagcaca ataaatccaa
    27781 aagatttatg caatatacct cttattattt caagacagac acttgttgat aaagcaattt
    27841 ctaaatggct tcaagacagt aatgaaaaac taaacattat tgcaacatac aatttagttt
    27901 ataacgcttc tcttttggta gaagaaggtc tgggctacgc tctgtgttta gatagactcg
    27961 tcaatactac agaaaacagt aatctttgtt ttagacctct atctcctcct ttagaagcta
    28021 aattaaatat tatatggaaa aaacatcaaa tattttcaaa ggcagcagaa aaatttttac
    28081 ttcaaataca aaaagatttt tcttctatca tgttggatac atctaagttg gatacaccta
    28141 aagaaaaata aatcataata aaaagtgaga gtgtctcaaa atgaactttg cactgtataa
    28201 ggcactcttt tttatacaaa gtaaaatata tgttcatcat ttcagcacta aaaaagaggc
    28261 ttatctctat tttgagatag ccccacttaa ccaataaaat taaatacaac ttaacaatat
    28321 ccttacttat ttattccaag ttctcttaga caagcaatag taatttcatc ttgtggattt
    28381 aattcataag ctctttctat atagtaagtt gcatcatcaa tatcaccatt atttaaataa
    28441 gccattccta aattgcaaag tatttctggg tcttccttta tcttagccgc ttgttcaaaa
    28501 tactctattg ctttatctaa attattctta taagcctcac aaagccccaa ttccactata
    28561 gtatccactt gattaggctt tattatcaat atcttctcaa gatacatctt agcctgctct
    28621 atttcgccca tacccttata tccaagtgct atcataaaca gcaagttcca ccaatccata
    28681 tgttcttctt ctaatggaag taacttctct aatccttcct taaacttacc ttggaataca
    28741 agtgtatatc cttcttcata ttgaacttta aagtccatct tgcccagatt ctcttgtacc
    28801 tcagctacta aatcagcatc taaccctagc cttaacgctt cttcccaagt cagcttagtc
    28861 tttaaatatt gaccttgatt ataataatgg tagcctaagt gataataacc taatgcaaaa
    28921 ttctcatcaa catcaattac cttttcaagc ttagccatgg cttcaagtaa gaatgcattc
    28981 attgccttat catccatatc tttttgatac tgtttagcta tctcttggca aacaatcgca
    29041 tagttataca ttgcatctaa atcatctgga tacattgtta taaatgcttt tatatatatt
    29101 aaagaatctg tatgttcacc aatctcaaag tattttctcg acatatatcc catatacgat
    29161 ttcaaatcca aatcaatatc ttttgataaa gcatttaaaa acttctcata ctctgcattg
    29221 tttttaaact tactatctat tccaattata tatatcattg catctgctat agacattgaa
    29281 tttaacttat cttgagcagt cttttctttt atccctttta ctaatacctc attttttata
    29341 ggtacatcta atccaccttt tggcacttca tagcttttta atttaaactc tccaccatgt
    29401 tttatagtta taaaagctaa ttcttctgtt ttttttagca aatatttttc tatcttaaaa
    29461 tccattttat cctcctaaat acttaaatct ctactttatg agttttagat ttctcaaata
    29521 tttttttatc atcatactca aataagacta cattttctac ttctcttatt tctgtattta
    29581 tatcattttt taaatccaca acattaaatc taaatggctc aaaatgaaca tattttatta
    29641 tatgtttagc tggtttatca acaaaactag gtaaatatgt tgataagtta tcattatctt
    29701 gtaaaaatac atggcatcta ttcttaaaat catccacatc tagcttatta aaaaactgtg
    29761 gtatatttga tgttttaccc agtgatatat agtcatattt taatatatcg ttgaatacat
    29821 cattatctat gttgattttt tcattataga aatccatcaa tattttatat aactgattct
    29881 ttccttgtgc caaatcaaag tagccatttt catcaaaata cttggcaaaa acctcaaaga
    29941 atttaaaagg gcttttttta taaaatctgc ctataatata tctcatagat aacacaaagt
    30001 ttttagaatt gtagtatctc tccagtatat cttctatatc ctttagcttc aaagtttctt
    30061 catagcttat ataattattg tataaaaact catatggagc ataatcctta tatttaaaac
    30121 catgctcatc tgcaactttt ctcatacctg tccctttaat catctttaaa aagcctaatt
    30181 gcaaatgctc tatacctaga ttaaatacat cattaaaaga attttcaaag ctcttataat
    30241 cttcataagg cagtcctgca attaagtcta agtgctgatg tatatttcta taagatgcta
    30301 cagtttgtac aacatgagac aactttttaa aatcatccct tctacctact gcatcaagtg
    30361 ttttttcatt cgttgactga acacctattt caaattgaaa aagaccttct ttacaatcag
    30421 ccaaaaactc aagcatctta tcatctatca aatacgctgt aacttcaaaa tgataagttg
    30481 tatatccatt atcatttttc ataaggaaat ccatgatttc catagccact tttctatttg
    30541 cattgaatgt cctatctata aactttattt gtgatactct tgcatctatt aaagctttta
    30601 aatcactctt tatcctgtct aaactaaaat accttagacc tggaatagta gatgataaac
    30661 agtattggca gttaaaaggg caacctcttg atgattcata atacactatt ctattttcat
    30721 attcttttgg attcaagttt tcataaggac taggaatctc atccaaattt tgaagtaatt
    30781 ccataggctt attcactatt atctcattgc cttgtctata tacaagtcca tttacatcat
    30841 ttatttccat ctttccttgc aagtgaagta ctaagtctct aaaaattaat tctccctctc
    30901 cataaagtat ataatccaca aaatcatact ttttcattgc ctcataacta tcataactca
    30961 cttccggtcc gcccaaagct attttagtat taggtctaat tttttttaga ttatcacaaa
    31021 gtttaactat atctcccaca ttccagatat atgttgagaa taatattata tcatattcat
    31081 ttttatatat atcctttaaa atatagtcca aatcgttgtt tatcgtgtat tctttcatat
    31141 taacttctat caaatctctt acaaattctt ttagatatct tatagccaaa tttgtatgaa
    31201 taaacttgga atttaatgtt gttaataaaa ttttcaaata tttctcacct ctttttaaga
    31261 ttatactgtc tttttgtacc ccttataggt taaaatagta tatgtaaagt ctaaataata
    31321 tctatataat taatttatta atttcaagta atgtgaggaa aggaagtttt ttatggaatt
    31381 aattgaaaaa gtatctatca actctttatc aaaaagagat ttactactta ttataaagtc
    31441 tcttgaatac actaatgaaa atactcaaat tagtgatttc attgacctta ggaataatat
    31501 agtaaaggaa ttatgttttt taactgacac taaggaaaaa gattttttag attatctaga
    31561 aaaaaatagc acactctaat aacctaaacc tttttaaact ttaatatata aatacctgat
    31621 ttagaattta tttttatgct taatatatta tcattaaagg ttttcgacat aactgttatt
    31681 tgattaaata tagggtctat tatagtatta ccttcttcaa gtcttataag acttcttggg
    31741 tctatgcttt tattatatgc cttaactcca attttatatt ttctcaattt aacaacatat
    31801 tcggacatag aaaaaataaa atctatatta tcttcattta atttactatc ttcacccaaa
    31861 atggataata tagatttttt tatgctatta attccttcat tttctacaat tctatcttcc
    31921 atatatacat catatagaac atttaaaaca tttaataaaa tactttcttc atcataatct
    31981 tcagatttca aatcaatgta cttatcaatt aattgtattg ctttaatcct tgccatctga
    32041 aactttacaa catctgattt gtctaattct atagaaaatc ctattatctt gtctttaata
    32101 ctctgtagta attcgacata ctctatttta ctattattta taagattgtg cataatggaa
    32161 ttatacacca cagaacctaa taaataatca aataaaagtt cttctttttt aaaataaata
    32221 acatactttt ttattagttt aataacctca tcttcacttt tttgaatatt agtatttgat
    32281 aaaattagtg gtaataaaat atttaaaaaa ttgatatttc ctattttttc tccataaaat
    32341 ctatagtaaa taagttcatc tctcagtcta ttttcgtcta tatacacaca tctttgtgtt
    32401 ttatcatcta ttaaggattt tgtatttatt atagttaaat ctttaagagc cctacttgca
    32461 ctactaaaag aagatgtgta gacttttttt agtaattcgt cattcataaa tatatccttt
    32521 ctgaaaaatt gaatgtaaat aaattgttat taaaaaaaaa agagacagcc cgtttaagaa
    32581 gctgtcatat atagggggat agtagtgtac aaaaatcatt gtaatctact atctatatat
    32641 tattatacga tactactttt ttatatgtaa agcatcagtt ttcgacatcc ctatatttta
    32701 cagttataca aattgcgtac taagttcaat tttattcttt ttatttcaac attttatcat
    32761 gctacattct ttcaggagcg tgcattccca acaatttcag agaattttct aaagtttgtt
    32821 ttgttgctaa aactaatgca agtcttgctt ttcttatttc caaattttct actaaaattg
    32881 gattgtcatg ataaaattta ttaaacgctt gagctaaatc tagcataaat ctagtcacta
    32941 tatgtggctc atttttcttt agagcaagta aaatattctt attgaatgat tctataactc
    33001 taagaacttc tgcactatct tcatcagcta atagactata atctatatca gaagtcactt
    33061 ctacttctgc ttttcttaaa actgcacagc atctagcatg tgtatactga acatatggtc
    33121 cagtctcacc atcaaaacta agcgttcttt cccaagagaa ggtatagtct ttaattctac
    33181 tattagataa ctcttggaat actactgccc caacacctac ttgcttagat atttcatcca
    33241 catttttagc atttgggttt ttagctagta taatttcctt agttttatct atagcttgat
    33301 ttaaagcatc ttctaaaaat actactcttc cttttctagt cgacatagta ccttcttcaa
    33361 gtgcaaccat accaaatcca acatgaatta aatcttttgc ccaatcatat cccataagct
    33421 caattacctt aaaccattgt tggaaatgta agttttgttg agaaccaact acatatatac
    33481 acttgtcaaa atcataagtt tcttttctat atattgcagc agctaaatct cttgtcatat
    33541 ataacgttga accatcattc ttagtaataa gtgctggtgg cattttatac tcttctaaat
    33601 caacaattct tgctccttta gactctttta aaagattttt ctcttctaat agttcgataa
    33661 ccctgttcat tttatcagaa taaaaactct ctcctgctaa agaatcaaat tctatatcta
    33721 aaagtttata aactctgtta aactctttta aactctcatt tctaaaccat tgccataatt
    33781 cttgtgcttc ttcgtcacca ttttctaatt tattaaacca tgctcttgct tcatcttcca
    33841 tttctgggtg ttgttcagct tcatcatgga atcttacgta taattttagc aattctggaa
    33901 ttggattaga ctctactact tctttttctc cccacttttt aaaggccact attaacttac
    33961 caaactgagt tccatagtca cctaaatgat ttattcttat agtcttgtat ccttgcgaat
    34021 catatatctt atataaagca ttacctataa ctgtagttct tatgtgccct atatggaaag
    34081 gttttgctat atttggagaa gaatattcaa ctattacagt tttatctttt ccaaaatcac
    34141 tatgtccgta attttctttt tcatctaaaa cttttttaat tacagtttct gctaattgag
    34201 atttatttac aaagaaattt acatatccgc ctaattgtat tacttttgtt atctctccac
    34261 ttggttctat actttccgcc aattcacttg ctatcatgtt tggagctttt ctaaacactt
    34321 tggctaattt aaagcatgga aaagcatagt cccccatatc cttatttggt ggaacttcta
    34381 taagtgcctc tatctcttct ttcgataaat cttctatttt ttctttaagg caatttgata
    34441 ttgccacctt aaaatcttgc attctgttgc tcctctctac attgttcctg gtttatattg
    34501 tgcttatctc tttatgttaa atcaaatttc gtgttttttc aagtatttca ttacagtgta
    34561 aaacacttta gtaattatgt tttctgtata tattaaaaat actataattg cagtttttaa
    34621 tcacactatt attgattttt tataagactt ttttctattt tattgtttct tgtactatcc
    34681 agttgacttt atatactttt tttaatcttt tatctatttt catacgtcaa tgctaatttt
    34741 atatactttt cttttaatct tttattaatt ttcatacatc aatgttaact ttatatacgt
    34801 ttttttaatc ttttatctat tttcatacat caatgtcagc tttatatact ttttttactc
    34861 ttttatctat tttctataaa tcaatattag ctttatatac tttttttact cttttatcta
    34921 ttttctataa atctttttca ctcttaactt ttattccttc ttcttctaat agccttgctg
    34981 ttataccttt ccctggtatc tttttacctg aaaaactacc atcatatatt agattacatc
    35041 cacaagaggg gctaccctct tttagtattg cttcgctaca tacatataaa tttgctattt
    35101 ttagagtttc attagcccct cttacaaagt tttctgttac atccttatta tctttagtaa
    35161 ttacttttcc atcaactatc tcagcaggtg gtcttggtgt agatagacca cctaactgct
    35221 ctgggcatac aagtgtaaat tctttgcctt ttaaatattc cttgactttt tcattatcat
    35281 tattatcgcc actatattta caatttattc ctattaaaca tgcagatacc aatatcataa
    35341 aaatgtgctc ctatacaaat tatatatatt ctcatacaaa ttactaatgt attcttatac
    35401 aaattatttt aatttaacaa ttgttactcc tgctccacct tctccatatt gaccacctct
    35461 ttgtgatttt acatgtctat ttcttcttaa aatgtcttgt agccctgctt ttaatactcc
    35521 tgtacctatt ccatgtatta ctgttacaga ctctaaacct gccacatatg catcatcaag
    35581 atacttttct acttccatta tagcttcttc taggtttaac cctctaaggt caacttcatt
    35641 ttttacacta ccagattttg accttataat gtttctcgtt gatttagtta catttgttga
    35701 aacatctttg cgtgtttttt gtaaagattt aaaaggtaga gtcatcttca ttataccaat
    35761 ttgcactact gcttcttttc tctttttatc tactgaaact acactaccat tttgatttaa
    35821 agttattacc ttaacttctt cacctggctt taagtctttt atctccttat ttgatacctt
    35881 tggaactatc atacttttta ctgttggctg aagacttccc atagagctag tcaattcttt
    35941 tctaagttct tctatcttac gattcttttc ttttgaagct ctttcttgtt caagacttct
    36001 aagttctttt attataatat ctacttcttc tttagcttgt cttataattg aaaatgcttc
    36061 tgatttagct ttttcaatca tcttatctct ttgactgacc aatttttcaa gtttttcgtc
    36121 atactctact ttcagttttt caatctcttc ttttaatctt tcagcttctt ctctatcttc
    36181 tactgctttg attctattct tttcaacatt ctgtaacaca tcttctaaag ctatgttttc
    36241 tgtatttata tattctttag cacgtgaaat cacataatca cttagcccaa gttttctaga
    36301 aatctcaaac gcatttgatt ttccgggaac acctataagt aacttatatg ttgggcttaa
    36361 agtttctata tcaaattcaa ctgctgcatt ttctacacct gatttagtaa gagcataatt
    36421 tttaagctca ctatagtgag tggtagctat acatttagct cctacagaat ttatatcttc
    36481 taatactgct attgcaaggg cagctccttc aacagggtct gttcccgctc ctaactcgtc
    36541 aaatataact aaagagtctg ctgttacgtt ttgtaatata gatactatat tcgtcatatg
    36601 agatgaaaat gttgatagac tctgttcaat actctgctca tctcctatat ctgcaaatac
    36661 attatcatat acacacatac tacttccata atcagcaggt atatgaagtc cactttgtgt
    36721 cataagtgca aatagaccta cagtttttat tgtaacagtc ttaccacctg tgtttggacc
    36781 tgttatcacc aatgtatgga agtctcttcc taaataaatt gtatttgcta ctaccttctt
    36841 tttatccagc agtggatgtc ttccattttt tatgtttaaa tatttatcct catttaaagt
    36901 tggttctatt cctctcatct gtatagaaag ttttcccttt gaaaatgcaa agtccaatct
    36961 tccaagtatc tcttgattag aaattaagtc ttcactaact tctccaacca tagcagaaag
    37021 ctctgataaa attctttcta tttcctcttg ctctcctagt ttcaactgcc tcaattcatt
    37081 gttcatctct actatagtca ttggctctat aaaaagtgtc gcaccagaag atgactggtc
    37141 atgcactata ccagctactt gcgacctata ttcagatttt acaggtacta caaatctatc
    37201 acctcttaga gaaattattg catcttgtaa gtacttttga taagtagttg aacttattat
    37261 tgaatttaat tttgacctaa tggattgatt tttttgggct attcttcttc tgatatctct
    37321 aagtgtactt gaagcattgt cagatatttc tacttcacta attatagcat tgtatatctg
    37381 gtcttctatg tccttgtata catacagaga atttgataga gattgtatta ttggataatt
    37441 aaaatcttct tcatcactac tagataagct atttcttaat cttctagcca ctcttaaggt
    37501 atctgctatc ataatcagac ttcctgggtc aagagaagca cctatataag ctctctttac
    37561 cttatcttct atatcatgaa taccttccaa ccctactgaa cctctcttta taataattga
    37621 ttgagcttca ctagtttctt ctagcataga tttcacttct acaaaatcag tatttggtac
    37681 taaattttct atatatttta gacctaaaga agatgaggct ttcttcttta acaaatctat
    37741 tattttattg tattctagaa ctcttaacga tttctcattc attttctata ttcaccatcc
    37801 tcgatatttt aactttttta tttaatttta aaaatgtatg ttttcaagca tttccaataa
    37861 aaactgcaag ttttatttct ttttcatttc taaaattatt atattttttc tcttttagat
    37921 atattttaca catattttgt attatactat gtatgtagct atctatatat tatacataaa
    37981 catgctttat ttgtaatata atctgttaaa aaatcttctt taatattttt aaaggagttt
    38041 attttagctt ataaaaaaaa atagctactt atatagataa attaaggagg aacatgagtc
    38101 atgggaaacg tattagaagg attaaaacca gaaagtgttt ttaaaaactt tgagaaaata
    38161 tctcaaattc ctagaggttc tggaaatgaa aaaggcataa gtgatttttt acttagcttt
    38221 ggtaaaaatt taggtcttga aactattcag gatgaaagct taaacataat aataagaaag
    38281 cctgctacaa aaggatatga aaattgtcca ggtgtagttc ttcaaggaca tatggatatg
    38341 gtttgcgaaa aagaaaagaa tgttgaacat gattttctta aagaccctat aaaattaaga
    38401 attgatggtg acatgatata tgctactgga actactctag gagctgacaa tggtatagct
    38461 gttgctatgg gtatggctat attagaagat aatactttag agcatcctgc actagaagtt
    38521 ttagttactg ttaatgaaga agatggtatg aatggtgcag atgcactaga ccctagctta
    38581 attaaaggtc aatatatcct aaatatggac tccgaggagg aaggatactt attagttagc
    38641 tgtgctggtg gtaaaacttg tgttgttagt ttacctgttg aatacaagga agttaaaggt
    38701 gacaaacaag gattattagt tgaggttact ggactattag gtggacactc tggtatggaa
    38761 attgttttac aaagagcaaa tgctaacaaa gcaatagcta gagtattaag tgtattgaat
    38821 gttgattatg aattagcttc tgttgatggt ggtactaagc acaatgctat acctcgtgaa
    38881 gctaaatgtg taatagctgt taataaagct gatgttgaat ctgctaaaaa acaaataaat
    38941 gacattttaa ctgcatttaa acatgagttt actacttctg accctggcat gacttactca
    39001 gtagctgaaa cttctgtaga caaagttctt actaaagatt gtaaagaaaa agttgttcaa
    39061 atgtcttgct taactcctca tggagttcaa tcagtaagtc ttgatataga aggattagtt
    39121 gaaagttcta ctaacttcgc tataattgaa actaaagaat ctactattga atttttaact
    39181 tctgttagaa gttctgttat gtctattaga gatgaaatcg ctgatagaat aagacttttg
    39241 gctcaagctt taggtgctaa ttacgacctt atagctcaat acccagcttg ggaatttaaa
    39301 aaaggctcaa aattagaaaa aatatgcagt gaaacttatg agaaattaac tggtaaagtt
    39361 cctactgtta tggctctaca tgctggtctt gagtgtggat tattacttga taaacttcct
    39421 catgctgaag ctatctctat aggaccagat atgtttgatg ttcatactcc aaatgaacat
    39481 gttagtatcc cttctgtagc taatgtttgg gattatgtta ttgaaatatt aaaatctatg
    39541 aaccaatatt aaatttaagt tttgattcta agtagatttt aattttaagt aatctatata
    39601 aaattaactc tgtaaattaa tactactgtt agtataatat ttactttcaa tattaactta
    39661 tagaaataaa gcctaatgat agataataac atgtatcatt aggctttatt gtaatttaaa
    39721 tattattaag ctttattctg ttttaaaatt aattcttata ctattttaaa tctatttctt
    39781 ccttgtctct tagaatcata aagagcagta tctgctattt gatacagttc atcatactct
    39841 tgaccaggct ttgctataac tacccccata ctaatagata aactttctcc atttggcaac
    39901 ttaatgagat gaatatgatt gtatacatca ttacatttat taataagttc ttccttattt
    39961 attatatcat tgatataaac actaaattca tctccaccca aacgacctac tatatcattt
    40021 tttgcaaaac tttcatcaag tacctttgca acatcaataa gtactttatc tcctaacaag
    40081 tgaccataag tatcattaat cgacttaaaa taatcaatat ctataatcaa taaagcacca
    40141 tttttttcag ttgataaatt caacaaattt tgtgcagtta attttctaat atatgctgca
    40201 ttataaatat tggttaaact atccctttga gccttatcta acaatctatt cttttcctct
    40261 ttttcagaat caatattaca tattttcccg attacataca ttggtatttt tctatcatta
    40321 taaattattt ttgaagtaac tctaatccaa tgaaaagtat catcaagcat aatagaatac
    40381 atttcagttg ttccattttc ttgtgatttt attacatcaa aaaacttttt attatattct
    40441 ttccttattt tatctctttc attttctata ttgccatttt cactttcctt atcattttta
    40501 tccaaaccat aataagtctc aatacccttt tctggcatag aaattatcaa tttatcttgc
    40561 ttataatcat actcaaaaaa gtattcactt gtcatttcat acaactgagt atgttttttg
    40621 agttccaaag atatcttctc gttcatactt gtacgagtat gtaaaccaaa tgccataaat
    40681 ccaataatac aaattaataa actggctaca ataaaaatag tctcaattgg attctgttgc
    40741 ataatataaa gcaacgagaa atcttgtcta tatgtagtat tttggtagac aattgattgc
    40801 attttttctg atgaaatact tataatagct ttattaaata tattcaataa ttcttgttta
    40861 tcttttttaa ttataccaaa acaaatactc tttgctttat aggtttgagg aactagacgc
    40921 acatttttat acttaggttg attcatatag tactgcatag aataaccttc gccataagtg
    40981 taatctgcat agcctttatt aacagcttct atacactctt ctaaattatc ataccaaact
    41041 atattctctt caaactgacc ataataatta atacctttgg ctagagctac acgttttcca
    41101 tctaaactat tctcatttaa tttattatta atcatcatag tatattgagt agaaagaaaa
    41161 ggccgagaca tagctatatt atgttcacga gagtattcat aatcataaat cattcctgct
    41221 acaatatcaa tttcacgctt tttagtcatg ttttctaaat cttcttgaga ttttacaggc
    41281 acaagttcaa attctaagcc tgttttatca catatatgat ttaacaaatc aattgatata
    41341 cctcttaatt cacctgtatc tttatctata ttttgaaatg gtggtttgtt taaaagtact
    41401 cctactttta atttttctgt ctctttaatg taattagttt cttcatcaga taaaaacatt
    41461 tgattattct ctggtgtaaa gtatttttca tataaagtag ttgaaaaata tgggtctttc
    41521 tgttcaatat tattaattgc tgagttaagc ttttgagtta aatttgtact acctttagga
    41581 gtaatgaaat aaaatggttt tgatgaaaat tttgctacag tacgtacacc ttctattggg
    41641 ttcatactca cattaagaag tacatctgct ttaccactct ttagtgcttc tagttgctca
    41701 tcatttgttt tacaatatac caattcaggt gacataagat tcattttaca atactcatct
    41761 aattctttaa gtctagtatc agaattttct attacagcaa tacggatttt tttcacatct
    41821 tgtgaactca ttatgttatc aatatctgta ttttcatata atgcttgtag tactgtattt
    41881 gctactccat aattattccc tgcataatta tatatttttt gcattggttc attgtataac
    41941 atacctccca ttatatcaat ttcaccattt tctaacatct tcattaattt tgtaatagat
    42001 tcatttatct ctccaggaac ttgaacaaat tcataattcc atccagtata ctgtgcaatt
    42061 tctagtaaat attcatatgt atacccaaaa tagttacctt gctcatctat ttcagttaaa
    42121 cctttttgaa taggataagc aacacgaatg gtacgattat cattacctat agcgttgata
    42181 ccaaaagaaa atatgcaaga agtgaatata gatgtaatta caataattaa taaaagtaat
    42241 ttttttaaat tcactacaca atcctccata tagttaagat ttacagtata aattactgca
    42301 taaacttgta gacatgtaaa aatagattag tatataaaag gatatataaa tctacttaga
    42361 aatttaaata tccttaatat atacaatata tttaaattat aactcattta tatgtctatt
    42421 acaacaaatt taccctgcta aatacctact ctatatccca taattttaca agttgtagtg
    42481 aagtttaagt tctatctcac ctcaaattta gcagtgtttc tatttacagt tcttttatat
    42541 ttcacatctg gataacccaa tataaatgaa gtgataacag cttcgttctc gctcattcct
    42601 aacagttctt ttatctttgg ctctacattt gatgccataa caaaaaatcc attataacaa
    42661 attcctaatc cttgagaata ggccataagt tccatattag atgcagccaa tcctccatct
    42721 acataagcac ttccacctag agacatatca cctacaacta ccataagtag tggggcatca
    42781 taaaataaag aatcatttcc gttctctttg tatcttttgt acattttctt aaatgtattt
    42841 ttatacatag aacgtacagg gtcattgtca tcagtatcta atgccaaatt ataaagacct
    42901 tgtattgcca gttctttcac ttcttttagc ttttctttta ctaatatgta tcttataggc
    42961 tgtctattac cacctgttgg tgtgtatcta ccagcttcca ctatgttttt tattttttct
    43021 tcttctacct ctatatcttt atattgtctt atacttcttc taaatttaat aaaattcaat
    43081 aacttttcgg gttctaattc aaaactttca cgattgtatt ctattacttc atttttatca
    43141 aatccttgca tttctattgc atctgttggg caaattgcca tacagtgacc acagttaata
    43201 cagagaatat ttttaacctt tgctttatca tccttaactt ttattgcatt tacaagacag
    43261 tctgattcac acataccaca gcctacacat ttttctaaat ttatgtctat cattccaaat
    43321 tattcctcct tttaaaaaat ttactttagt ataaaacaaa tagttgtcta agtcaatttt
    43381 tattttttta acaatatact agccttaact acactttaat ggagaaaaat atacataaat
    43441 tggaacttaa attagaactt attcaaagta aaagataaaa gaagtagatg tctaaaagtg
    43501 aactttttag ttaattagat attctttatt gtatgaaatc aagtggatat ttatcatttc
    43561 aacaataaaa aaggcttacc tctattttgg atatgtccct gtcaagtaga cagactaaaa
    43621 aaaggctatc tttatgcact gagatagccc cttttcccaa ctactattgt ttattttaat
    43681 attatatcaa taaatttaat ttgctataac ctgtaagtta cattttaatt tgatgtaact
    43741 ttaaattaca tttttaagta gtatctttaa attatattct taaaatatat cattaaacta
    43801 cattttaatt aatctaaaaa ctatatctta attaatctaa gaaacttaca ttataatctt
    43861 ttagtttccc attatcatat acaatctttg cactaaagat atttccagtc ttagcccaat
    43921 ttaagaacag cctgtcacct tcccacatat ttaaatcgaa tattttatca ttatcaaccc
    43981 aaattaaatt tccttcttta caatcattta aatctttttc agataattca ccttcaaact
    44041 catctgagaa atacaaaaac atataccaat cttcttcatc tttaaatttt ggaaaagtta
    44101 tcaacccttt atatgacaat gatttaagag taagaccagt ctcttctttt acttctctaa
    44161 ttacacattc ttctggagtc tctccttgct caaactttcc tccaactcct acatacttac
    44221 cttcatgtat atcatctttc tttttatttc tgtacagcat caaagtcttg tcatcttttt
    44281 ctatataaca aatagttgta agtttcatgt atctcgcctt gctttctctt ttatattctc
    44341 aactaattat tttcagcact ttcaagcaca tcacttatat tcttcattgc attaagagaa
    44401 agctctgcaa attcatcaaa tggaatccct atatcttcta tacttctcat tgcatctcta
    44461 tttacatttg aagcaaatct cgtttctttc attcttttaa taacagactt agtctttaca
    44521 ctggtaagtt ttttatctgg ataaactaaa gctatagcag ttataaaccc agttataggg
    44581 tctgctgcat atattgcttt ttccattagg ctagttcttg taactcccat tccatcatga
    44641 tgaccaagta ttgctctata catttcttcg tcaccgaagt tttctttttt aagcagttct
    44701 actgttgttg tagcatgtcc ttcacatatt tctcctggct ttcttccaac tatatctgaa
    44761 tccaaatcat gtaaaagacc tgccactgcc cactcttctt ctttttctgg ctctagtctt
    44821 ttggctaatt ctctcataac agcttcaaca gcataacaat gttttctcat tctatcagtt
    44881 tctaaatact taaataaaat ttctaaagct tcttctctca ttttcaccaa tccccctgtt
    44941 ttttcacata tatgatattt ttatatatta atacacataa attcaagttt ttatatatat
    45001 tcatgtatat ttttcatgtt atatctattc tacaccttta tagtagtgtc ctgcaactgt
    45061 tccaatttca tctaatttct tatacaagtc tgtagccttt tttccaagtt tataattatt
    45121 actcaatacc tcaatgtgag cttcaataac ctttttaacc atctcactat cttcaactgt
    45181 aaaatctatc ctaaatacat ctattccaat ttcctctaac tcatatagat tgtccaacat
    45241 acaagtaacc ttcgaattgt aaattgtact tctacagaat atatcttgtg ttatcctata
    45301 acttgcatcc gtaaagtctc ttaaagcata actactttcc ttacatttag caactctctt
    45361 gtccttttta caatctctaa caactactcc cataggacag tattctgtaa ccatcatagg
    45421 agcatatcca tatattactg cttcaatttc tacatctgta tattttaaag tttgtttaat
    45481 ttcatttaag tttagttctt gtgacaaaca aactgtactt gctccctctt ctttgaagtg
    45541 atttatactt tcactattga atgcatttaa gtagcaatct atatttaagt cttttttctt
    45601 aaagtattcc atagagcccc agttaccaat ttgaacctta ttaattcctt ttatattatc
    45661 caatatctca aattgcttgt actctttatt tctaacaatc cttggtgctg aatagattaa
    45721 ttttttgtca gtgactgctg tattatagtg gttcttattc aaagtatcta ttttacttgt
    45781 atttgacaaa gtatctatgt tatttgtatt tgaacccatt agttctttag gagtacctaa
    45841 agacgaagaa gtatctaaat ccatagaaat acttgaatct atagaaatat ttgaatctat
    45901 agaaatactt gagaccattt gtaaagcttt tgataatgta tttatatctt cataatagat
    45961 taaatctaca ttataattta atactgcttc taattgctcc aagttcttaa ctttaactct
    46021 aatcttagga gctgtatcaa gttttttctg tttatttact ctatttggct ggtattttaa
    46081 tctattcttc ttaaattttc tatcttttat ctttattctt tcttcatcta aaagctcaat
    46141 acaatccctt ctcatctgat ttaacacact tataggcata cttacacctt catccaggtc
    46201 aatttctatc tcttttaact tatatggtgt atttccaagc ttttgaattt gtgtttctat
    46261 tttttcttta cttaaagcca cctttatagc tttttcaact attttgtctc cttctaccac
    46321 cgatttattt ccacaaaaat cacttataaa aagctctggc tttttaccta gtttaatact
    46381 cacttttgca cgtatattag tttttatgaa ctctttatct tgctgaaatg tagcttgtac
    46441 tctatccatt aattcgctgt cagaagtctt atatataagc tgatttctct ttgcttctcc
    46501 aacaaaatct aattctatca aatccccttt atgtgctatt gtagttatct ctccatcctt
    46561 tattattctt ccaatagtac ctccacctaa gttaattcca tcacctttct taagcgtatt
    46621 ttctagcatt actctaagtc ttttgttctt tctattataa tctaaaactt ttccaatata
    46681 tagcccttga ttattaggtc tattagagtt cattatatcc ttaccaactt cacctaatat
    46741 atatcctttt gtaaactttc tattaaatat ggtatacaaa ttatttatta tttcctcaga
    46801 aatacttatt tttttcttat ctacaaattc attaatcgct tctctatatc ctgaaaccac
    46861 agtagctaca tactctggct ttttcattct accttcaatt tttaaagata atacacctgc
    46921 ttcaattatt ttatccaaat cttctatagt acttaaatct ctaggactca acaaataatc
    46981 tccattactt tttactactt ctcctgtatt tatatctatc aactcatact tttgtctaca
    47041 tggctgtgca cacctacctc tatttcccga cctattgcct atcatactac tcataaggca
    47101 ttgacccgag taacatacac ataaagctcc atgtacaaaa acttctatat ctacagttgt
    47161 attttcacaa atccatttta tctcatcaac atttaattct cttgctaaaa caactctatc
    47221 aaaccctaca ctctgaaggt attttacatc ttctaaggaa tgtgctacca tttgagtgct
    47281 tgcatgtagt tcaaagtcag gtaatagttt ttttataagt cttgccattc ctatatcctg
    47341 tactattaaa gcatcaatat ctatatcgta taaaaatttt acatactcta caaaatcttc
    47401 tatttcatac tgttttatca atgtatttac agtaacaaag acttgcactc cccttatatg
    47461 agagtattta acagcctcaa tcagctcatt tctatcaaag ttatttgcag atgctctagc
    47521 actaaagtct ttaccaccta aatacacagc atttgctccg ttttgaactg ctgcttttaa
    47581 tgaatcaaaa gagcctactg gtgcaagtaa ttctattttt ttcatatttt tctcctctat
    47641 ttatacttgt taggttaata taattatatc tataacttaa atatttgtat taaatttata
    47701 tatttcttac tatattttaa attcttcaat caatttatat attagttatt tatatttggt
    47761 tcatttgagt taacatatac attatttact atatttagta tatttctatt aacttatata
    47821 ttgattact
//
LOCUS       DAELNY010000010        45292 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.010, whole genome shotgun sequence.
ACCESSION   DAELNY010000010 DAELNY010000000
VERSION     DAELNY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.010"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(123..1478)
                     /locus_tag="KPE55_000479"
     CDS             complement(123..1478)
                     /locus_tag="KPE55_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG0491581.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(1504..1773)
                     /locus_tag="KPE55_000480"
     CDS             complement(1504..1773)
                     /locus_tag="KPE55_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0491582.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(2063..4018)
                     /locus_tag="KPE55_000481"
     CDS             complement(2063..4018)
                     /locus_tag="KPE55_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491583.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNITGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKNIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIDVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PSLNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDDSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(4201..5676)
                     /gene="spoIVA"
                     /locus_tag="KPE55_000482"
     CDS             complement(4201..5676)
                     /gene="spoIVA"
                     /locus_tag="KPE55_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG0491584.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(5848..7142)
                     /locus_tag="KPE55_000483"
                     /pseudo
     CDS             complement(5848..7142)
                     /locus_tag="KPE55_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(7326..8735)
                     /locus_tag="KPE55_000484"
     CDS             complement(7326..8735)
                     /locus_tag="KPE55_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0491585.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(8787..9926)
                     /locus_tag="KPE55_000485"
     CDS             complement(8787..9926)
                     /locus_tag="KPE55_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0491586.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            10245..10895
                     /locus_tag="KPE55_000486"
     CDS             10245..10895
                     /locus_tag="KPE55_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491587.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(11052..12071)
                     /locus_tag="KPE55_000487"
     CDS             complement(11052..12071)
                     /locus_tag="KPE55_000487"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0491588.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(12102..12716)
                     /gene="plsY"
                     /locus_tag="KPE55_000488"
     CDS             complement(12102..12716)
                     /gene="plsY"
                     /locus_tag="KPE55_000488"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG0491589.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(12751..14070)
                     /gene="der"
                     /locus_tag="KPE55_000489"
     CDS             complement(12751..14070)
                     /gene="der"
                     /locus_tag="KPE55_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG0491590.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(14104..15432)
                     /locus_tag="KPE55_000490"
     CDS             complement(14104..15432)
                     /locus_tag="KPE55_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG0491591.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(15670..15870)
                     /locus_tag="KPE55_000491"
     CDS             complement(15670..15870)
                     /locus_tag="KPE55_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491592.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(15872..16804)
                     /locus_tag="KPE55_000492"
     CDS             complement(15872..16804)
                     /locus_tag="KPE55_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0491593.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(16849..17781)
                     /locus_tag="KPE55_000493"
     CDS             complement(16849..17781)
                     /locus_tag="KPE55_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0491594.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(17836..19125)
                     /locus_tag="KPE55_000494"
     CDS             complement(17836..19125)
                     /locus_tag="KPE55_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491595.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(19154..19846)
                     /locus_tag="KPE55_000495"
     CDS             complement(19154..19846)
                     /locus_tag="KPE55_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491596.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(19861..20622)
                     /gene="pgeF"
                     /locus_tag="KPE55_000496"
     CDS             complement(19861..20622)
                     /gene="pgeF"
                     /locus_tag="KPE55_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG0491597.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVTIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(20619..21080)
                     /gene="nrdR"
                     /locus_tag="KPE55_000497"
     CDS             complement(20619..21080)
                     /gene="nrdR"
                     /locus_tag="KPE55_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG0491598.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(21102..21365)
                     /locus_tag="KPE55_000498"
     CDS             complement(21102..21365)
                     /locus_tag="KPE55_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0491599.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(21469..22242)
                     /gene="sigG"
                     /locus_tag="KPE55_000499"
     CDS             complement(21469..22242)
                     /gene="sigG"
                     /locus_tag="KPE55_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG0491600.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(22334..23056)
                     /gene="sigE"
                     /locus_tag="KPE55_000500"
     CDS             complement(22334..23056)
                     /gene="sigE"
                     /locus_tag="KPE55_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG0491601.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGILGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(23081..23953)
                     /locus_tag="KPE55_000501"
     CDS             complement(23081..23953)
                     /locus_tag="KPE55_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG0491602.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(24243..25517)
                     /locus_tag="KPE55_000502"
     CDS             complement(24243..25517)
                     /locus_tag="KPE55_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0491603.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(25801..26958)
                     /gene="ftsZ"
                     /locus_tag="KPE55_000503"
     CDS             complement(25801..26958)
                     /gene="ftsZ"
                     /locus_tag="KPE55_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG0491604.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(27148..27501)
                     /locus_tag="KPE55_000504"
     CDS             complement(27148..27501)
                     /locus_tag="KPE55_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG0491605.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(27600..28274)
                     /locus_tag="KPE55_000505"
     CDS             complement(27600..28274)
                     /locus_tag="KPE55_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0491606.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(28279..28977)
                     /locus_tag="KPE55_000506"
     CDS             complement(28279..28977)
                     /locus_tag="KPE55_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0491607.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(29000..29740)
                     /locus_tag="KPE55_000507"
     CDS             complement(29000..29740)
                     /locus_tag="KPE55_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG0491608.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(30010..31236)
                     /gene="murG"
                     /locus_tag="KPE55_000508"
     CDS             complement(30010..31236)
                     /gene="murG"
                     /locus_tag="KPE55_000508"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0491609.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSASKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(31254..32384)
                     /gene="spoVE"
                     /locus_tag="KPE55_000509"
     CDS             complement(31254..32384)
                     /gene="spoVE"
                     /locus_tag="KPE55_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG0491610.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(32436..33791)
                     /locus_tag="KPE55_000510"
     CDS             complement(32436..33791)
                     /locus_tag="KPE55_000510"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG0491611.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(33810..34778)
                     /locus_tag="KPE55_000511"
     CDS             complement(33810..34778)
                     /locus_tag="KPE55_000511"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG0491612.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(34819..36192)
                     /locus_tag="KPE55_000512"
     CDS             complement(34819..36192)
                     /locus_tag="KPE55_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG0491613.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(36359..38338)
                     /locus_tag="KPE55_000513"
     CDS             complement(36359..38338)
                     /locus_tag="KPE55_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG0491614.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(38350..38697)
                     /locus_tag="KPE55_000514"
     CDS             complement(38350..38697)
                     /locus_tag="KPE55_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491615.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(39178..40113)
                     /gene="rsmH"
                     /locus_tag="KPE55_000515"
     CDS             complement(39178..40113)
                     /gene="rsmH"
                     /locus_tag="KPE55_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG0491616.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(40143..40889)
                     /locus_tag="KPE55_000516"
     CDS             complement(40143..40889)
                     /locus_tag="KPE55_000516"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG0491617.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(41283..42569)
                     /locus_tag="KPE55_000517"
     CDS             complement(41283..42569)
                     /locus_tag="KPE55_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0491618.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(42584..43849)
                     /locus_tag="KPE55_000518"
     CDS             complement(42584..43849)
                     /locus_tag="KPE55_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0491619.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            44165..45262
                     /gene="ychF"
                     /locus_tag="KPE55_000519"
     CDS             44165..45262
                     /gene="ychF"
                     /locus_tag="KPE55_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG0491620.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        14100 a         8385 c         4753 g        18054 t
ORIGIN      
        1 caactgattt ttaaagttta ttatcttaac aaagttcatt atcttatata atatttaaat
       61 gcataggctt ctttaattta accatctaaa tatttaatta aatattatta tatgtaatat
      121 aactaatttt taaatgaatg tataggagct ggaatccttc ctcctctttg agtaaataat
      181 tcagatgaat ttgtatttat aggcattact ggtgctcgtc ctaaaagtcc tccaaattct
      241 accatatcac ctacatcaca tcctggggct ggtataattc taacggctgt agtcttatta
      301 ttaatcatac ctattgctgc ctcatcagct atcatggctc caagcgtacc tgctgtagta
      361 tttcctggca cagctatcat atcaagacct acagaacaaa tagcagtcat tgcttcaagc
      421 ttatctatac ttaaagcacc tttaataaca gcatcaatca tacctgcatc ttcagatact
      481 ggtataaacg ctccactaag tcctccaaca tgtgaacatg ccattactcc acctttttta
      541 acagcatcat ttaaaagtgc taatgctgct gtagtcccat gagtaccaac aacttcaaga
      601 cccatttcct ctaatatatt tgctaccgaa tcaccaatag caggtgtagg agctagtgat
      661 aagtctatta tcccaaatgg aacatccaat ctactagaag cttcctttgc cacaagttgt
      721 ccaactcttg taattttaaa tgcagtcttt ttaactgttt ctgaaacaac atcaaaaggc
      781 tctcccttta ctttctctaa agctctctta acaacaccag ggccacttac accaacattt
      841 ataattctat cagcctcacc aacaccatgg aaagctcctg ccataaatgg attatcttct
      901 actgcattac aaaatactac taattttgca caaccaaaac cttttgcatc ttttgttaat
      961 tctgcagttt cttttatgat ttctcccatt tctttcactg catccatatt tataccacat
     1021 cttgtagaac ctacatttac tgaagcacat actttattag tacttgctag agcttttgga
     1081 attgacttaa ttagtatctt atcacctttt gtatatccct tttgaactaa tgctgagaat
     1141 ccaccaataa aatctatacc taaatcctct gcagccttat ccaatgtctg tgcaaacttt
     1201 acatagtcat tttcatctgt tgcacctgca ataatggaaa ttggagttac tgataccctt
     1261 ttatttacaa ttgggatacc aaattcacgc tctatatctt ttccaacctt caccaaatct
     1321 ttagcagaac tcataatctt attgtatatt ttttctctag ctttatctcc atcagggtct
     1381 atacaatcta ataaagaaat ccccatagtt atagttctta tatccaactg ttcttcatct
     1441 atcatcttta ttgtttcaag tatatttcct acattcacta ttatcacctc cccttactgt
     1501 ctactacaga ttatgcattg aattaaatat ttcttctcgc tgaattttta catccactga
     1561 taatttctta cctctttcaa ccaagtcctc aaccgttttc tcaaaagaat ttaatgattt
     1621 ctctaaatca actatcataa tcatagtaaa ataatcctgt aatatttttt gtgatatatc
     1681 tactatgttg atattttgct tgtataattc atcactaatt cctgccacta tacctggttt
     1741 atcctttcca attactgtta aaattgcttt cataataata tcctccctta tttttttata
     1801 aatcttatat ttttacaaat tgaactatgt gttaacatat atttcattta tttgttagta
     1861 aaccactttt tatgtatata tttaatatat tttaatacct atacattcat ttgtcaattt
     1921 tttttcaata ttctattatt tttatactat ttagaaaatt catatattat atatgttttc
     1981 tcaacaataa atacagtttt cataataatg aaaggtatta cttagcaatc tcatgatttt
     2041 ctaaataata cctttctaaa aattattttt ttaattttat gaatttaatt atactttttc
     2101 tagtatcagg atttacaatt aatatagaac caatcataat tggtgttagt atacaaaata
     2161 aaaaaataat atcttttttt agattatatt ctttagattc taatttataa ttattattta
     2221 ttattccatc ataatcactt atatttgact tatttttatt tgtatcttga tttttgttag
     2281 aaatactttc cctataagga aaagaatgtt cattggtacc aaatactgat tttgagctta
     2341 aatcataatt atcatcattt tctgtaattg aatttttatt gtaaaatcct ttttctttat
     2401 cagaattatt ttctttatta ccgtctttag gtatatcatg aggattttta ttttcattgc
     2461 tatcagtact tgattctaca tcatctttag gagtatttgg ttgtttctca tcagaatcat
     2521 cattttcatt attattattt tcattattat tgttttgatt gttattacta tcattatttt
     2581 catttccatt attcccatct tgattgttat catcctcatc tctatccggg tcgtctctac
     2641 ctcctcctcc acggtcacca tcaatatcat cattttcccg ttcatcatct ggcgtctctt
     2701 ggttgtctcc gcctcccatt ataaatcctg gtttaaaaac tatatttgaa tacccttctt
     2761 ttaatcctcc tacaactttt actctaaaac aacgcaactt aaaatcttca aaaaaaagta
     2821 tagcttgatc tctaatatcc tcctgctctt cagatatcca attgattcca tcgcttgaat
     2881 attcaacaaa aaccttactt gcactatacg gcatatcata aaataaaaca gctacatatc
     2941 cattttttgt attttgaaat tccagttcca aattgtctat tggttttttc tctataacag
     3001 atacattcaa actaggaact acatcatcat ttattataat tttttcttta tttaacattt
     3061 ctgttttaaa ctttcctgtt atattaaaat cttctccctt ttccaacttt tcataataat
     3121 cagtaaaatc ccattctaca tctatatcaa gtcgattacc actagatgtt tctaatacca
     3181 tatatttggg aaattttata ttttctattt tatcatcttt acttactttt atttcatcac
     3241 taaatacatc aattccccta tgacaacatt tcacaatatc atagccagaa tcttcagcta
     3301 tagagggtat aagctcatta taatcatcaa ttatataaac tttcttactt tctgatataa
     3361 ttgattgtgc caacttatta ttatctttta gtaacccact ttcattgttg ctactatatg
     3421 cttttattga agtttcttct atctcaacat cacctttaga ataaacacct atagcatgtt
     3481 ctccattcac ttcaatattc ttatttagta ttctaatatc attttcagta taaattccta
     3541 ttgcatcttt tccatttgcc tttatttttt caacagattc gtaggatgat ttaatatgta
     3601 agtctcctcc ttcttcaacg tataaggctg tcccatcagt agcaattatg ttgtttatac
     3661 caatacttat acttccacca ttttctatat gtattactcc ttcttttcct ccttcacctg
     3721 ttatattcaa ttcattatca tcaataataa atctcccatg attttttatc aatattttat
     3781 atttgttggt atctattgta aaattactgt cataaggaat ataaaatcta aactcttcat
     3841 ctattaccat atcacaagaa agagtataaa ctatttcttc tttattattt ttattttctt
     3901 gaacccacct ttcaaaatct tcaagacaat tttcctcttg tccaaaaact ttatgtggaa
     3961 aacttataaa acacaaaact attatactca taaaaataaa tatctcatac tttttcatct
     4021 tacactccta gaaattttta gctttgaaac aatcctgtcg aaacatatac ttcacccact
     4081 ataatttttc cacaattatc aattattgtc aatttatata agtatgttta ttaattgtta
     4141 taaataaaaa gctacttact aaagcaaaca gacgtttcac tttaataagt agctatattt
     4201 ttataacaaa atagttataa tattagaact tccttcattt acaatttttt gtaaagtctt
     4261 ttgcatttta actcttatct cttctggcat agtatataat ttactttgta attgctcttt
     4321 tactaagtca tgtagtgact taccaaacat atttgattcc cataaatctg ctggttgctc
     4381 ttcaaatact tccataagat attttatcat ctcttcacct tgattttgat ttccaactat
     4441 tggagaaacc tctgtagata tatctgcttt tataatatga agtgatggtg cattggcttt
     4501 taacttgata ccatattgtt ttccttgttt cattatttct ggttcttcca gactaagttc
     4561 ttctaaggaa ggagctacta ctccatatcc ttttatcttg gcatctatta aagcactttc
     4621 tattttatca tactcatttt taactttaga tagtctagtt atcagactaa gcagttgata
     4681 atctccttca attttaaatc cacttttttc ttcaaggaca ttatagaata aatcttgctt
     4741 tgtctgtaaa tcaatattta taacaccttc tcctagctct acattatcaa cacctgtatc
     4801 ttccaaaaac tctagctcag aaaatccttg tataatacct tctatatccc ttattttacc
     4861 tatatctata atactttgtt ttaatgttgt tattatgcta ctttttatcc aatgatttct
     4921 ttcaagacct tcaacccatt ttggtaagtt gattcttatt tctgtaagtg gaaaatcata
     4981 taatacagct tccataacct cctctatatc ttcttcttcc atttcaacca cattcattgg
     5041 aagaactgga acttcatact tttcttccaa ttcacttcta agcatactcg tttcctcact
     5101 ttttggactt aacgtattta aaaccactgc aaatggtttt ttaagatttt tcaattcttg
     5161 tataactctc tcttctggtt ctacatagct ttttctgtca attccagtaa ctgaaccatc
     5221 tgtcaagaca actatcccta ttgtagaatg gtcttttata acttttttag ttcctatctc
     5281 tgctgccttt tcaaatgtca tagcttcttg tgaccaagga gtggatacta atctttgttt
     5341 tccaccttcc tcatgcccta aagctccttc aactatgtat ccaacacaat ctaccattct
     5401 tacttttaaa gatactgtgt cttttatttt tatttctaca ccatctgctg gtacaaattt
     5461 tggttctact gtcattatcg ttttacctga accactttgg ggtatctcat ctcttgttct
     5521 atccttttta aattcattgt ctatattagg tataaccaac ttttccataa attttcttat
     5581 aaaagttgat tttcctgttc ttacaggtcc aactactcca atatatatat ccccctgagt
     5641 ccttttggat atatcttcgt atatgttatt attcatatta ttcctcctat acatctaatc
     5701 ttattaaatt atatttagag ttaagggtta atattcttaa aatagaaaaa taaagaccag
     5761 caatattaat ttaatttaca tatagaaatt tgtaaatttc aatgtaaatt aaattaatat
     5821 tgctggtcca aatagtagtg tattatatta actgacttta gtagtatttt catttgaatt
     5881 attattcatc tcaataagtt ctttacgtct ttcttttgtc atgttcatcc atataaatgt
     5941 aaacaccaat ccaattatgg ctactatagt aagaactatg aagtagtacc tatatccagt
     6001 tacacccgga aatgcatcca atatcttccc tgctgctaaa ggacaaagta cttctggaag
     6061 atatcctaat gtagcaacta ttcctatagc agttcctgat attgataatg aataattacc
     6121 ttcttcaagt aacgaatagt gtaaaccttg aattgcatac atagatacat atattgcaac
     6181 acatccaatc aatagtatat aaatcataga tgattttgtt ggtgtaaata ttacacctaa
     6241 aagacctgca atcataagta taaatccata agatataatc tttgatgaac caactttatc
     6301 tccaagtaat ccagaagcag ctgatgctac tggtctacaa tactgagcta atattgagat
     6361 tgcagatgca aacaccacag tagctccaaa tgattcagtt gaataaggcg taaaataata
     6421 aaatgacata ttagctgaat atgaacaaaa cattattatt gttattatcc atgtatgagg
     6481 catttttatt actgcaaaaa aggcttcttt atcaaatttt gctgatacct ctttttcatt
     6541 atcctttaat ttgaaaaata ctaatactcc aactattaca tttattgcga gtataatact
     6601 ataatagctg taagaccaga tttatcactt acttttgatg aaatataccc aaatagtatc
     6661 aatactagag atagatgaat tgcattggta attccacgac cactttccat aaatccaaaa
     6721 gcttttcctt gttcattttc agatgctaac attctaacag cttttacaag agctggccaa
     6781 aaagtaagta ttgttgttat tccccataag gcatggatta aaaacacaac aatatatgga
     6841 ggataaagtg ttaatacaag accagatatt cctgtaataa ttaaagaacc tgaaagcaat
     6901 tttctggctg aaatacggtc tgctaaaaaa ccaccaaaaa ggtatgatac cattgcaagt
     6961 agaccataaa tacttcctaa gcttcccatt tgtgtatttg taagattaaa tgcttgtgca
     7021 aaagtatcat aataataatt ctttaaatat ggtaaagcat atataaatgc tcctgctaca
     7081 aagagtatta tcaagacttt gaaatttttc caaatgcttg gtttatttac ttcaactgac
     7141 atacttcctc cttatgtaaa tacctgtaaa ttttcataat aattaattat tgcttatttt
     7201 attgtttatt caatatttag gaatatttat taatatatta ggttaatcta tcaataattt
     7261 atggtgagtt atgagctaaa agtaaactct tttagctcac cataataatt atatttttat
     7321 ttatactaac atccagagtt attagatatc tttttttcat agttcttcca tagcattaaa
     7381 cctgttatta taacaaaaag acctcctcct atcatttcaa tctcatagat tatattatct
     7441 tttaaagtct ccataactgg tgccgttgag aaaagtatgc caactacagt tactacaagt
     7501 accattctgg caatattgat acaaactgaa tcactttttg caattgaata tggtctttct
     7561 tcatttggtc ttgtctttct aagtctaata taagatgtaa aaagcaagac atatggaaat
     7621 aatgctgtta atgctgtcat agtaacaaga acattgtata tattatctac tgatggtaat
     7681 aagtttgttc ctacaagaag tagactcact attatagcct gaactattac tgcatttgat
     7741 ggtatattgt atttatttgt aacagtcaat tgcttaggaa atacaccttc tcttacattt
     7801 ccaaaaagca tctttactgg acctgctata taaagtatta atgctcctag tactgataaa
     7861 gttattccaa atgctactat tcttataaac caacttccta tacctaaatc ttgtgcaact
     7921 ttagctaatg catctagtat ccctgtagat gctgcaattt tatctggagg aagtatcatt
     7981 gttattgcta ctgaacctaa gatatagatt cctcctatta aaacagccac agttaatata
     8041 gctttaggaa agtttttctt agcattatcc atttcggtta tgaaattagc agtcgtttca
     8101 gctcctgata atgcaaatat tatagcagat atagaaacta atgaattggc atctattttt
     8161 ggtattatat tttggattgt atatatcgat gctgatggag cttttttgag aataactaca
     8221 gacataaatg ccatcactat aagtaatata gcaggaattg tagaacctaa tgctcctgta
     8281 ttcgtaaaaa attttccaaa tgccatccca cgagtactta ctaaggacaa cagccaaaat
     8341 attatgagtg aaagtgctaa aataaacatc ttattgtttg ataaatctgg atttccaagc
     8401 atatatgcaa tatttatcgc taaaaaagtc aagaaagaag aataccaaaa tatctttgat
     8461 gtccaattta gccatgacac cataaatccc cacttctctc cataagcttg tttaacccat
     8521 tctccaagtc caccatcttt tggatatgtt gcagccaact ctgcacatat aagtgatgca
     8581 ggaacaaaga atataaaagc aaacaataac caaactggta tagaaccaag acccaatcca
     8641 aaactagagg cagtagattt agctatccat ctaatcccaa atagtgctga tatattcata
     8701 aatactaaat cccaaaatcc aattttcttt ttcactgtaa ttcctccaat ttcaaattta
     8761 ttcagactca tacacaatgt attgacttaa atatcaagta taagttcttc aacacttctt
     8821 ggataattag ttataattgt agcaccattc ttacctacta ttgctgtatc tgagtgtcta
     8881 aatcctccaa ctccaggtat atatatccct ggctccatag aaaataccat tccttcttct
     8941 aatattaatt cattgtcaaa tcttaaatat ggctcttcat gttctgataa acctaaacca
     9001 tgaccaattc tatgatttga atataactct agaccatact ttgcaacaac agctcttgct
     9061 gcttcatcca ccattcttgc tggtatacca gcttttattg tatctaatcc agcttgttga
     9121 gcctctactg caatcttaaa cacttctttt tgtctttctg ttggtttgcc tataataaaa
     9181 gttctttcat tttctgctct atagttttca taccaaacct gtctactatg tattactata
     9241 tctcctcttt gaagtattct agtattggaa tctaaatgag gttgtgctgt tctatcaatc
     9301 cctgaacaag tccagtttgc aaatcctata tatgtatcag gataattttc agaaacatat
     9361 tttagtaaag cattatcccc tgcaacgtca aactccaatt cactcatccc aactctaaca
     9421 ttttctaaag acccttttat tcctatatca gataggtaac ctgctatctt caaaaattct
     9481 atttcttttg catcttttac atatctcatt tcaaatattt tacttccaac atctttgata
     9541 gcaaatgact tatcagttat atactctgaa aagcttgcag atactatatc tttttctatt
     9601 ccaacagttg ttcctttagg gtatttattt aaaattatat ctaagtaatg tttatgtgat
     9661 attccatgtt ctttcatttc tggtatttca taatacacat atacattatc aacttttgca
     9721 acttctttag catgtaattc ttctaatcca ggtactatta attctatttt atcttgagtt
     9781 actactgtta caattggtct agaataagtt attgctttaa atccactaat ataaaattga
     9841 ttgtctggtt caaatattaa agcaatttca atgctttttt cattcatgta ctttctcaat
     9901 ctttctagtc tcatattatt tgacataatg cacctctttc ttaatattca gataatttat
     9961 tcaatacaac aaggatgaat atattatttt aaaattctta atatttttag tacttgtcgt
    10021 atttgtatgt aaattaattt tatttgtgat tattttatca taatcatgat tattttgtca
    10081 atcatgattt tattttaaaa aattttttat attctagtga ttttaaatta tctaaaatat
    10141 aacgagtata cattgtattt tgtaatatta ttcttgattt atggtaagtt taatgataaa
    10201 atcacgatta tataaactat attgattatt tgtaggtgaa taaaatgaca aataaagaat
    10261 tacaaaagaa aaggatattg atgtatttca tagaagctgc tcaaaatatt atggaaaacg
    10321 aaggtataga aaatattaca cttcgtaagg ttgcagatat ggctggctat aatagttcaa
    10381 ctctatataa atactttaaa aatttagacc atttaatatc atttgcatct ataaaatatt
    10441 ttaaagatta taacttgaat atttcaagat gtatagaaaa tgtcaatgat gaatataaga
    10501 aatatataat tatgtggaaa ttattttgta agcattcttt tgataatgct caagcttttt
    10561 accaggtatt ttttaacttg tctagtgatg aacttagcta tataacaaaa caatattatg
    10621 atatgtttcc agaagattta ggtattcatg atagtgacat atctttaatg attacaggag
    10681 aaagtataaa ggaaagaaat aagatactat taaacaatct tgtagatact ggatatatac
    10741 cagctaaaga tttagacata atcaatgata taattatttc tttttatcaa aatctattat
    10801 tcttaaaaaa gaacacagga aaaaattctg atgatgatga attaactatg aaaatgattt
    10861 cttctattga atacataata aaacatgcta aataaaatta aaatatggct gtctcaaaat
    10921 agagataagc cttttttatt gttgaaataa taaaaatata tttaatttca tataaaaaag
    10981 agtgcctcgt aaactaaaaa gttcattttg agacacccct aaaataaatt tattatttat
    11041 tatacaatag tttatatatc atcacctaaa actacttctt ccatttcatg ctttttactt
    11101 ctcatcatta gttctattcc aacttcttta ggatttgaac ctttctttat tacagaatat
    11161 atagcattag ttataggcat atctatatct aatttttcag caacatcatg tgctacttca
    11221 gtagcagtta tgccttctac aaccatcttt acttctttta aagtatcttc taaactcatc
    11281 cctttaccta tgagtatacc agctcttcta tttctactat gcatacttgt acaagtaact
    11341 attaaatctc caatacctga aagacctgca aaggtagaca tattagctcc catagcaatt
    11401 ccaagtctgc tcatttcact aatacctctt gtcataaggg cagcttttgc attatcacca
    11461 taccctaatc cgtcacatat cccagctcca aatgctataa tattttttaa tgctcctcca
    11521 agttctactc caactatgtc tggattcgta taaactctaa gtttagggct cataaataaa
    11581 tcttgtatca tctgtgctat ttttaaatct ttagaagcaa ctactacggt agtaggaata
    11641 tcccttgcta cttcttcagc atgagatggc ccagatagta ttacatatgg attttgtggt
    11701 aattcttctt cacagacttg agataatcta agcccagtcc ctttttcaag acctttagct
    11761 acattaacaa taacttgtcc ttcttttata aatggtttta ttttttggca agtacttctt
    11821 acagcctgtg atggaactgc taaaactatt accttacaat cttttatagt tttttctata
    11881 tcagtactta tagttatatt atttggaaat aatactcctg gtaaatagtc tgtattttct
    11941 ttagttctgt ttattttttt agcttgttcc tcttttcgag tccacatgct cacatcatta
    12001 cctttttttg caagccctaa agctaatgca cttccccagc ttccagtacc taatacacat
    12061 actttctcca tgtttaacac cacctgagta taatatattt actattcttt cttttgacca
    12121 atttttctct ctgtcccatt taataatcgc tttatatttg ctctgtgatt atatattact
    12181 gatgcagtta agaacagagc tactattaaa ccagctttat tttttaccat aatcataaat
    12241 attggtgaac atcctattcc tacaacagaa cccaatgaaa catactttgt tatagctacc
    12301 accaatataa atactgctag acacattagt gttattactg gatttacagc tagcattgcc
    12361 cctagtgaag ttgcaacacc tttgcctcct ctaaatccta aaaccgctgg ccaattatga
    12421 cctgctacaa cacatataac tgccagatat ccagctaaaa gagtatctat tccaaccaat
    12481 cttgctgcaa attctgatat taaaacagcc attacacctt taagaacatc acctaaaaat
    12541 gtcattgctc ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc tgcattacca
    12601 gaaccttgag tccttatatc aaccccagca attcgctttg caactatgta tgaggtagat
    12661 atatttccta aaagatatgc aactacagca ataattatat aactaaatat ctccactgaa
    12721 tttcccccct ttagttatat tttaagatat ttatttttta tttttttgtc tatactcaaa
    12781 ctttatggaa gttccttcaa atccaaaatt ttctcttatc ttattctcaa ggtatctttg
    12841 atatgagaag tgagttaaat ccttatcatt tataaacaat gtaattttag gaggtctaat
    12901 gtcagtttga gtcccatagt aaatttttaa tcttctacct ttgtcagatg gtggttgatt
    12961 taacattaca gcttctccaa ttacttcatt cagtgctgaa gttgatattc tcttagaatg
    13021 ttcatttgac acatattcaa ctgtatctag tatcttgttc attctttgat tagtttttgc
    13081 agaaacaaat acgattggag catacatcat aaatggaaat ttttctttta tatcttttgt
    13141 gtaattgctc atagtcttat tatctttctc tattaaatcc catttattga ttacaaatat
    13201 gcatcctttt ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt ctgttacacc
    13261 ttctaaggca tctattacta tcaatactac atctgctcta tctacagcac tcatagccct
    13321 tattacactg tatttttcta ttgtctcata tattttactt tttcttctaa gccctgctgt
    13381 atctattaga agaaatttct gtccattttt ttcaaaataa gtatcaattg cgtctctagt
    13441 agttcctgca attggactta caattactct ttcttctcca agtatcttat tgagtataga
    13501 actttttcct gcatttggtt tacctgttat agctactctt attatatctt cttcatactc
    13561 tgtatctaat cctgctggaa aattttcaac tatctcatct aataaatctc caaatcccat
    13621 actattggct cctgaaactg caaaaggcgt tccaaatccc aactcataaa aatcgtatat
    13681 attatcaaat tgactttgac tatcaatctt attaaccact aagattactg gtttttttgt
    13741 ctttctaagc aattgtgcaa tttctttatc agcagctgtt attccagctt taccatcaac
    13801 tataaaaagt attacatgtg acatgtccat tgcaagcatg gcttgatttc tcatttgaga
    13861 taatattata tcttcgctat caggttcaat tcctcctgta tccactaatg taaaatattt
    13921 atctaaccat tctacttcag caaatattct atctcttgta actccaggtg tattttcaac
    13981 tattgatatc ctttttcctg caaatttatt aaatattgta gacttaccaa cgtttggtct
    14041 cccaactaca gctacaactg gcctacttat actcatttta tttctccttc catcttttgt
    14101 tttctatttt aaaattttat ccacaaaatc ttttccttca ttcaaacaag ggataatttt
    14161 tattctcata agttcttcta attgatttaa ttcaatatta tctaagaaaa tttcttcatc
    14221 tgcttttagc atacttcttg ttatataaag agcttctcct aattctttat cttcaagttg
    14281 ttcttttaaa tccttagctg ttaaaagacc tgatacagtt attgtctctc caaaaaactt
    14341 gtttttgatt tcatatacat ttacttgaag atttttaaac ttgtccatca tagcatctgc
    14401 catactctgt ataaattcat atgcagaatg accagtagct attgatactt tcttctctct
    14461 ttttagaatc ttctcaggca atatatttag atactcttca atctctgttt ttaacttacg
    14521 tatcatacct acaccatctt caaactgtaa aaaaccttca tattcttcat atccaggcaa
    14581 ttcactatta gatagtatat aaaattcatc tgataaaaat gcaaatcgag tacctaattt
    14641 ttcaagatat ttctgttgta tttgatgtat ttgctctata gttttacttg cgctcttatc
    14701 attaaatata tttaattcta ctagattttc tctatgcttt gtaatcccaa ctggaactat
    14761 cgcaacactg tttacatacg gataaagttg agctagttct tttattgttc tatccaattc
    14821 ctttccatca tttatacctg gacataaaac aatttgacag ttcatttcta tatgagcatc
    14881 tgccaatctc ttcattatac catataattt tcctgcaaat ttattgctta tcattttttg
    14941 tcttaactct ggattagttg tatgcacaga tatatttata ggacttattc tatattttat
    15001 aatattattt atatcttctt cactcatatt tgtaagtgtg acaaaatttc cttgtaaaaa
    15061 agataatctc gaatcatcat ctttaaaata aagagtttct ctcatacctt ctggcaattg
    15121 gtctataaaa caaaacatac acttatttct acagctttta gcttggtcaa ttatcggatt
    15181 tgtaaattca acacccaaat cttcatcata gtctttttct atctcgtata tgtagacttc
    15241 gccatctttt ttttgtattt ctacatctaa atattcatca cttagtaaga atctatactc
    15301 tattatatca tgtattggct gttcattcac agatataagt aaatctccta cctctatacc
    15361 catttcttct gcaatactat ctttataaac cttacttata atattattta ctttttttac
    15421 atttacttcc aatattttca ccactctttt gtattcatca atgcttatta taatgtattt
    15481 taatatgcaa tttatttatt tattatattt tatgatagca tttttaagca tttcatgtca
    15541 taataacaca ttttaattct aatagtcaat ataagtaaat agctataaaa tagattagaa
    15601 caaataatat tcaaatttaa gtttcttaaa aatttaaata ttatttgttc ttaaaaaaat
    15661 tttataaatt taatgtttta ctatgattaa cgtcccattt gtcatatcat gaaccattcc
    15721 acctacacac tttgacatca acaatgcctt attttgtaag tctttctcat catttgcata
    15781 aaatattgga caacctccac catttttcat attcttatca agtgtaacta tagctagggt
    15841 atattcattt aaacctatat ccattcccat ctcatgcact tcctttcttt cctattttat
    15901 aattttttag tgataagttt tttcctttag agcttgaaag tattgggcat gactttacag
    15961 cttccattac tttttccaca ttcttatcta ctggtaaata agcaatcatt atactttcat
    16021 cctctttatt tcttctaggt aatggtacga atgaaggttc tccataatct ccatataaac
    16081 ctaaccttga atatatattg tatactattg cttgtctttg accggagtca aataatattc
    16141 ctgcattagc ataatcctta tttttaggaa ttatttgtat tcctacacct ctattaatat
    16201 atacttctct atcgcttttt agacctatat ttgttatacc ttttaaatct cccaccttta
    16261 gtatagattc gtccacaaaa ctaatttcag caggcattac atcagctatg tctccaatac
    16321 tttttctagt aagaagtttt tttaatataa aggctaacac taatccacaa attgaactta
    16381 ttaccaaact catcccttca cttattttcg tttctgcaag tattacataa tacaatccta
    16441 ttgttaaaaa agatgtaact atacacatat aatttcttac ttcataggtt ctagctatct
    16501 cttctatgaa tgcagctcct ctctttacaa gttgaacttc ctctaaattt tgcaatgtat
    16561 ttcttctatt tgctctcact tgtctaaatt gttctgctgc tagagataaa aaagttatag
    16621 atgtataaga acgatttata agagctggaa ccaataatgc tcctaatgaa gaagcgacga
    16681 atgctaatat aagttgagat agtaaaacat ttggttgcgt tggatactgt ttttggtcta
    16741 aattcaacat tataattcta gatgttatac caataataac agcaacaaaa aatactctat
    16801 ccattaaatc actcctaaaa tatttaattt gaaattttac atgtaaatct acaaagtctt
    16861 tctagaaaaa tcactctgct ttccttttga tgtttctatg ataggtgtac ttttcactac
    16921 atctatcatg acatctatat ctttgagtat tggcatatat ggtataacca cagtactttt
    16981 ttctaaattg gtcttagata tagcaacaat atcaacctca tctacatctc tatctatccc
    17041 catatgaata aatagattat gtattatagc ttgtctttgc cctatatcat ttactattcc
    17101 aaatgcacct aaatctcttg gtataacttc tatggccaga cattcatttc tatacttttc
    17161 tcttgtactt tctagaccta tgttagttat tattacacca tttaccatta aaataggtcc
    17221 ttcaaaactt atttttgcag gaactatatc tgctatatct gctatactgt tccttcttaa
    17281 aaatcttcta aaaatcaaac caacaatagc tcctgacaca atagctagga ttgtgcaata
    17341 aaaaaagcta aggccatact ttcttgcaaa aacgatataa actatcgaag ataccagtgc
    17401 agaaaaaaga cttatataac ttctagattc atacgtagat gctatttctt caacgtaagc
    17461 gtctcctttt ggtactaatt cttcattatc gatattcttt agtgttattc tttcttgttc
    17521 tgcaagacct tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg caaactcctt
    17581 atctataagt gcaggtaatg ctatagcacc taacgaagct gaaagcccag atataattat
    17641 ctgttctaaa taatcttgtg gtcttgatgg gtattgtttg tctgtaactc ttaaaactaa
    17701 tcctctacat aatattccta caactaatgc aactataaaa gagtgtctaa ataaatttgc
    17761 attcaataaa ctttcttcca ttttctcagc tccttctctt atatagtttg tgcaaaaaac
    17821 aaaaaaaatt cccttctatt taaggaaatt tttttgtaat attgtaacta taaattcgct
    17881 accaatgcca agttcacttt ttacttttat acatccattt agtgatttga ctatatgctt
    17941 tgttatagcc aatcctaaac ccgttcctcc tacatcccta cttcttgctt tatctaccct
    18001 ataaaatcgt tcaaatatgc gttctacatc ttcttttggt atccctacac cattgtctat
    18061 aactttaatt acgatttttt cttcatcttt taacactaca acttgtacaa tcccattatc
    18121 ttgggtatac tttatagcat tatcaactag gtttaagaac acttgcttta tatagtcttt
    18181 attggatagt atacttattg atttatcatc acatttatat aaaacttcta tatttttttc
    18241 actagctgaa tgttgagtta tttcatatac ctctttaaat acatcatata ctacaacatt
    18301 ttccataaga agagtatctt tattttctac aaatgataac agcaatatat catcaatcaa
    18361 cctttttaat ctatctgatt cgctttctat tatttctaag aatctatttc tagtattcac
    18421 atctatattt tcatttgact ttagtgtttc aacaaaccca cttatagatg taagtggtgt
    18481 tttaagttca tgacttacat ttgcaacaaa atcacttctc atattttcaa gtttaacttt
    18541 tttagtaata tcttctatat ttactatcga acctattatt acgttgttag tattctgaag
    18601 ataaactggg tctactttta tcttatatac gatatcatct ttagtagtaa tattagcagt
    18661 ttcattatct ttagaaccaa taaattgaag tatttgtttt aatatttctt cttcttttat
    18721 tacaacatta atattatgac cttcaactaa ttgatttggt tcactcttta tcatttcttt
    18781 tgcttcatca tttatcagaa gtatatttcc atttacatct atagctaaaa ttccatgaga
    18841 tatactcttt aatatagacc ttaactgtaa atgtttatac tgtacttctt ctaaggtttt
    18901 atccatattc tttatcatat gattaaaatt tctagctaat tcgccaagtt ctcctttggc
    18961 tgatatgtta agcctagcat gatattcatt attacttact ttttttgaga cgtatataaa
    19021 ctcttttaaa tattttctta atgttattgt gtatcttaca gctaatataa caatggctat
    19081 ggacaacata acaataaaaa aatatatact atttaaatct tccacaagat aacactccta
    19141 aaatataacc tttttaatct attttgtatc ctacacctct tatagtctct atatattttt
    19201 ccgatttatc ttcatcttct attttttttc ttaaatatct tatatgaaca tctactgttc
    19261 tagtttctcc atagtattca tacccccata ttttatccaa aagataattt cttgaaagta
    19321 cttttccttt attttgaatt aataatttta acaattcaaa ttccttaaga gttaactcta
    19381 tcttttcaga gcctttggta acttcatgtt ttgataaatc tagttttaaa ttacctgtag
    19441 taagtatatt gttttcttca cctaagctag tcaaattata tctccttaaa acagaactaa
    19501 tcctagctaa caactctttt atactaaatg gcttagttat atagtcatca gcacctattt
    19561 caagcccttc aaccttgtca ttttccatat ttttagcagt aagcattata acaggaatat
    19621 tttttaagtc cttatcactt cttatttttc tcaaaacttc tattccactt atatttggta
    19681 acatccaatc taaaagtatt aaatctggtt taatttcctt agcctttata aatccgtcaa
    19741 atccatcata tgaataacta acttcgtaat ttgacacctc caaattgaat tttaatagct
    19801 caactatatg catttcatcg tctataacaa gcactttagt attcattaaa aagctcctct
    19861 ctattttatc tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt ttctataaga
    19921 gtggaattta tctgctctac aactagtgca taaatttaaa tttattatat tttctttttt
    19981 aactccacta catctaagca tatactcatt aattttccat aaatctaaat agtaacttcc
    20041 ctcttttatt atatagaact tttcatcatt atttgtaaga attgtgttaa atttttctac
    20101 taaatcttca gacacttcat aacaacatgg tccaattgat ggtccaatta tacatactaa
    20161 atcttcagga actgttttat agcattcaga cattaatttt attgttttat gacctatatt
    20221 actaaaagtc cctctccaac ctgcatgagc cagtcctatg gctttatttt tagcatctat
    20281 aattgatata ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt ccaagtttgt
    20341 tataagtgca tctccatctc ttcttttccc tatagtatct ttattgacta tagtaactat
    20401 atctgaatgt atttgagaat tactagttag attttcaaat gtcaaatcac attctttaca
    20461 tacttttaac atatcttctc tattttttgc atctatattc ttacatgtta taactagatt
    20521 tacagaattt aatttttcat caagttcttc acaattacca cttaaattaa catactcttt
    20581 aatatcttgt acatttttta tatttatata atctttcatc atttgtctcc tttttctatc
    20641 agtatacttt taagttcatt tacaaaagta tttatatctt taaattgtct atacacagaa
    20701 gcaaatctta cataggcaac ctcatctaac tctttcaatt tatccattac catctcaccg
    20761 atttttttgg tttcaatttc ttctataaaa catttattta tttcattttc aatatgtaca
    20821 acaatacttt caatctcgtc aatagaaaca ggtctttttt cacatgcttt tagcaatcca
    20881 tattttatct tttctctatc aaaatattct ctactattat cctttttaat gaccattaaa
    20941 ggagttgttt ctattttctc atacgtagta aacctctttt tacatgattc acactcacgt
    21001 cttcttctga tagattttaa atctgtatgt ctagaatcta ttacctttga ttctttataa
    21061 ttacaataag gacattgcat ctatactcct cctttgttta actatatctc aaactcatct
    21121 aagcttaatt ctgaacctat atttactatt attgtatcac aacctatttt aagtatatca
    21181 ttccaaaata taacttcttc atcccctcct cttgaaaaaa aacttctact tccatctcca
    21241 aaaatggtaa atgagactac cttaccttcg tgaatatcca tgtctatgtc tattataaac
    21301 cccatccttt ttccattttt tacatttata atttcttttt ccattatatc agaaactcta
    21361 atcatattgt actcaccctt tctttaaaaa taaatatgta tcgaagtaaa aaaaaatgac
    21421 ctatataggt catttaaatt aaaataatgt ttacagtcca tggattactt atacatattt
    21481 tctcatattt tttaaagcat ttttttcaat tcttgatact tgtgcttgag atataccgat
    21541 ttcatcagca acttctatct gagttcttcc tttgtaaaac cttaaatcta ataccaactt
    21601 ttctctacta tttaatttct taatagcttc tttaagtgat atttcttgca accaattttc
    21661 atcagtatct tttttatctt gaacctgatc cataacgaat attgcatctc cattgtcttg
    21721 atatactggg tcaaacagag atattgggtc ttgtatggca tcaagtgcca ttacaactga
    21781 ttctacttct aattctaatt cctttgctat ttctgatact gtaggttctt tagaatttgt
    21841 tctaattaat ctttctctaa cttgtagtgc cttatatgct atatctttta atgacctact
    21901 tactctaatt gggttattgt ctcttagata ccttctaatt tctcctataa tcataggaac
    21961 agcataagtt gaaaacctta cattttggct taaatcaaag ttatctatag cttttataag
    22021 accaatacaa cctatttgaa ataaatcgtc tatattttct cctctgttgt taaacttttt
    22081 tattacactc aagactaatc ttaaattccc tcttacaaac tgttgtctgg cttcttcatc
    22141 accatttttt atttgtaata aaagttcctt catttgctta tttttaagaa ctggaagttc
    22201 tgatgtatta acaccacata tttcaacctt attaacttgc atatattttc agtcctctct
    22261 caaaagtatt catctattga aattatttac atttccaatt tttttatact ttttaagaag
    22321 aattaattta gcattataca aattttttca tttctttttg caaccttgag attattttct
    22381 tttcaagtct agatatgtag gactgtgata ttccaagtat tccagcaact tctttttgtg
    22441 ttttttcaat acctttatca ataagaccaa atcttaattc cataatttgc ttttctctgt
    22501 cacttaatct gtcaagtgcc ataaccaaca aatctctatc tatttcttct tcaattattt
    22561 tgtatatttc atcattttct gtacccaata catcagataa taaaagttcg tttccatcta
    22621 aatctatatt taaaggttca tcaaatgata cttctgtttt ctttttatta ttttttctta
    22681 agtacattaa tatttcattt tctatacatc tcgaagcata agttgccaat tttatatttt
    22741 tattcagttt aaaagtatta actgctttta tcaaacctat agttcctatt gaaatcaaat
    22801 cttctacatc tatacctgta ttttcaaatt ttctcgatat gtagactact aatctaagat
    22861 ttctttctat taaaattgac tttacacttt catctgtttc tagcttttgt aaaagctcca
    22921 tttcttcttc tggtttcaat ggaggaggaa gtatatttgc ccctcccata tagtatattc
    22981 cctttggctt tattattttt aatttaatac ctaacatagt aataaagctt attattcttt
    23041 ctttcaatcg taacattcct tgtccccctt aaattttcac ctaaaatcca ggcaatatat
    23101 ttggattcat tattgctcca tattctctat cattaaaatt tgaaatacca agtattatat
    23161 ttcctatctt ttgctcatct acttctatat agtcagcttt taagcctatt attatgcttg
    23221 tcttattact tccagcatgt ttataaggta ttattctaac tctacctgaa gttttatcat
    23281 ctaaacttcc tataatttct tgtgctttct tcatatctat atcttcataa ttaatattta
    23341 atagtttttc tggaataaat ccttctaaca cactagactt aactattatt acatcacttt
    23401 gacttattgg gtcttttaac aaattaccac tatctactaa agccttacag caaaaacttt
    23461 tttctagtaa gtttatattt atagtctttg taagctcctt aatgtactta agcattttca
    23521 aatctttata aatgtatttt aataattcac aactcacata agtacatgca attaagaaag
    23581 atatttttaa gtgacttatt cctgtaaaat aaattataaa ataagtgctt ccagaaataa
    23641 aaatgtttac tagataaaaa actaacgcta catgaacata atcttttttg ctcttacttc
    23701 taaatgaaat taaagttatt atactcatga ttattatttt aaatggtaca gtaaatagta
    23761 tattcattga tggatacaaa taagcaacag aataggcacc tcctaaacta gcccccaata
    23821 acttattttt tttaggatta tatcttttag taagaataga agtacaactt ataataatat
    23881 aatttattac taggttttct ataacatagt actcaatata caccatgtat ccccccttaa
    23941 aacctttaaa catattataa gatagtatgt acaaaatttt atgtcacttt tgatggtcga
    24001 atccaaaaca aattctattt aaaaagaaca atgatatatg tcttatactt tatgtcgtca
    24061 aaagatattt ttttaatgcc aaacaaatag acttttttgt agaatccaat atatctaggt
    24121 agaagttatt cgctctactt ttggtatatt tacaaaaaaa gactacataa tttaataata
    24181 tatcaagtaa atcttttact tatataaaat caaattatat agtctgtaaa ttcaaatata
    24241 aattattgtg ctagtaatcc atttgcatat tttgcagatt catttagtgc ttgttctgga
    24301 gttatgtctc cagctatcat catttgaagt tgtttccata aatattcata tatctctact
    24361 ccatgaggtc caactgtcaa tggtctatac atatgtttat ctcttgtcac catttcttca
    24421 aatcttgggt ctccttggta tttttcacct ttagcaactg gagctctagg tatttctttg
    24481 tggaaagctt ccattgattc tttgcttagc atatactcaa gaagtttaaa tccaagttct
    24541 ttatttttac atgctgacat taatgttaaa tcatctactg aagcaaaact tccaactgtc
    24601 ttatcttcta atgatgttac atatccccag tttaaatccg ggaatgattt atcaaaagta
    24661 cttgtagcag cagaggatag cattatagta aatgcagctt ttcctggtcc aaatacactt
    24721 tcaatcattt cattactatc ttttgataaa gcatcttctg gaatatactt tttcatatca
    24781 tttataaagt ttgcagcttt caagccttct gcattattaa atttaactga ttttaaatca
    24841 tcattatata tatctccacc agcttgccat aaatacggat accagttcca gtttaagctt
    24901 ccaaatactt tagaacccca accttgtgca agaccccact ggtcgatttt tccatctcca
    24961 tcagtatctt ttgttgcttt ttcacagatt cttttaaaat catcccatgt ttttggaggt
    25021 ttttctccta atttttctag tatatcttta ttgtagtaca atacgcctgg attagcagct
    25081 tctataggta aaccataaat accacccatc atttttgcat catctaagta tagataatta
    25141 tctgaagttc ctgatttttc taaataaggt gttaaatctt ctaccgcacc catttctata
    25201 aattgaggaa acatctctgc atacatgtat cctatatctg gaccttcacc tgcacttatt
    25261 gctgttgcat atttttctgc gtagttatcc catggtacta tctgaaattc aatttcacag
    25321 ttattttctt tttcaaattt ttcaaatatc ggcttccatg catctgcatc atggtctgtt
    25381 aatggtgcag accatacaac taatttttct ttaccctctt tgcctttaga atcttctttt
    25441 tttgaacatc ctgcaagaat tgatactgat agaatcccta ccataactaa agaaagaacc
    25501 ttttttagtt taatcatgct taatcccctt tcagacttta atttgtcttt ttcctcaaat
    25561 gtttacattt aattcagatt taatattaac aaataagatt actcttgtct aatcataaaa
    25621 aactatacta actcctataa ctatattttt tttctatacc tattagattt aattcatact
    25681 aaaaacttac tttttaaatt ttatcaatat ttaaaaatat ttttaagtct agccacaaac
    25741 taactatcta aattgaacaa aataaaaaga agtctattga cttctttata agcttacata
    25801 ttatcttctt cttcttagga atgtaggtat ctccatgctc tcatcatcat cttcttcaat
    25861 tatacttgcc tttttaggag gctctatttt ttcctcagca attacttctt cttcaatttc
    25921 ttttacagct tgttttactg ttgtatttaa tgaagaacgt atgcttgatt taggtttagt
    25981 atctaaatct aaggcaccat tttgtaatcc ttcaaatcct gtcgcgatta ctgtaatcat
    26041 tatttcatca cctaaatctt cttttataga agctccaaat ataacatttg cttctgggtc
    26101 acaggattcc ataactaaag ttgatgcctc attaacttca aataatccta agttaggacc
    26161 acctgtaaca ttaagaagta ctcctttagc tccttgtatt gatgtttcta gaagtggaga
    26221 ttgaatagct tgtcttgcag catcaattgc tctagtttca ccactagcac tacctatacc
    26281 catatgagca agtcccttgt ctttcattat agtagtaaca tctgcaaagt ccaagtttat
    26341 taacccttca actgctataa ggtctgaaat tgattgtata ccttgtttta atacatcatc
    26401 tgcaactgca aaagcatcta acattgatgt atttttttgt actatctgta aaagtctatc
    26461 atttggtatc gttataagag tatctacttt tgatttaagt tctgctattc caccttcagc
    26521 gttcttcatt cttatcttac cttcaaatgc aaaaggtttg gtaactacac ctactgtaag
    26581 tatccccatt tcttttgcca agcctgcaac cactggagca gctcctgtac cagtaccacc
    26641 gcccattccg gctgttacaa agaccatatc agctccttga agtaacttta ctatttcatc
    26701 tttactctcc tcagcagctc ttttccctac ttctggattt gctcctgctc caagtcctct
    26761 agttaatttt tctcctattt gaactttata ttcagctttt gatgtatata aagcttgttt
    26821 atctgtattt actgatataa attcaacgcc ttttagttga gcttctacca ttctattgac
    26881 agcattgttt ccaccgccac ctacacctat tactttaatt tgtgcacatt cttctaattc
    26941 tacgtcaaag tttagcatca taaaccccct tctctcatga ttcaaagtta tcgtatgtat
    27001 taattatatc attttcttct tttattttgt taacctttaa gtaacatatt ccacaaaaac
    27061 gtaaattttc ctttttaaat ttcttactta tattgtcaaa tattactctt taagtataaa
    27121 ataatttacg tatatattaa atgctcttta atttttacgt tttttgttca taatattact
    27181 aataatatat cgtctaatta tagataagtt attaaacaat ctcataccaa aaactaatac
    27241 agcaacatag taaagagaaa ctcctaactt ttctcctata taagtcaata atatagccaa
    27301 tatagcatta cctatgaaac cagtaataaa aattacatta tcatagttat cttcaaaatt
    27361 ggattttatc gctccaaaaa tagaatccat tattgcaaga atagctactg acatatatat
    27421 ggaataatcc atagaatatg taaatggaat atagtaacct actactacac caaatgctaa
    27481 tcctcctaaa acccataaca taaatcttca cctctttatt ttactttgaa acacaactac
    27541 attacaatcc tttttattaa ttttttttac tttttcttta tttttttgta tttttcgttt
    27601 tattttgcaa ataaattcac tttatttcca ttattttcct gaacttttat attaattcca
    27661 tatatatcct taagagattt agcatatgat tctggagagt ttatagctgc actcaataaa
    27721 tctatatctc ctatagcttt tattataaat ggttgtccat atgtagtatc atttactgta
    27781 atagtagctc ctgaacattt tatcttactt gttgctagta ctctttctcc atttatagaa
    27841 attgctcttg ctccagcctt ttttaaatca tttaaaaccc tcaaaatatc tatatcatgt
    27901 ataatcaaat cgtttggatt ctgtccgtct tttaactctc tctcactatc cctcatagta
    27961 atagttatgc ctggccctgt aacagttgaa taaccactta actctttaac ttcttcaagc
    28021 tctttttgca taagtgtttg gatagattca tttccattct tttttgcttc tttatattca
    28081 ttcaaagttt ttttgttctt tttaagagtt tcttttaaat tttttatttc tttttttgta
    28141 ttttctacct cactttcaat actttcagtt tgacctaatg ttatgtatac tctatttggg
    28201 tctagtgatt ttatataagt tgatatgaaa acgcctaata tgaatgctcc taatataact
    28261 attttctttt tcatactatt atttcccctc tgctttgttt atatcactat gaccatttat
    28321 ttctatattg tcttctttgg tcacttttac tttggcatta taatatgttt ctatttccca
    28381 gacaatacca tactttatat taagtgccga agccaatgta tcagaatcac caattgcttt
    28441 tattataatt ggttctttta tgacagtgtc attcatataa agcttgtctt cttttaaata
    28501 catatatgta ttagaaacta ttctctggtc gtttatagat atggcacttg cttgtgctga
    28561 atttaattta tttatcattg acaataataa atcataatta tatagtatac tatcattttt
    28621 atcagagttt cctaagtctt ttgtagaatt tatctcaatt tttatgcctg ttccttcaac
    28681 atctgtatat ccagctaatg tttcatattt ttctatttct gacttaagta tgtcttgttc
    28741 tccttcattg tttttgcctc tataagtctc tatatctttc ttcacactat taacttctga
    28801 ttgtagattc tctttttctt tttttaaaga tttaagttct tctgtaagtt gctctcctct
    28861 tttaaaagtt gtcatccctc tattttctat attaataatt ttaagttgta aactaacaag
    28921 aacacccaat acaatacttc ctatgattat acttttattt ataaatcttt ttttcaatat
    28981 atcacttcct tacaattgtc tatccttctg gactatacac tgccaacttt ccatagttaa
    29041 gatttatagt ccctccatta gtatttttat tttgcaggtc aatcagaatt ttacttaccc
    29101 tagatatatt ataatctaag ttatcatcat tagaaagtaa gatttttata ttacttcttg
    29161 taagcatttc tatattgctt tcttttttta agtttacata atttattttt ctataaattc
    29221 cttcttcttt taaataattt aaagttttaa atacattctc cttagtttta taatttttaa
    29281 attttataga tttattatca tcaatgctat aatctacatc tactatcaag tcttttttac
    29341 tttcattaga acctcttagt tcttccaata aatttccttt tttatctata taacaataat
    29401 tatcttcatc ttttaatact gcaaatattt ctttttcttt taaacttata attatcttat
    29461 tgggaagttt tctttttatt tccacatttt ctatatatgg attttttata agtttatttt
    29521 tcatatcttt aaaattgtat gcaaaaatat tttcatttag atttacattt agttctttca
    29581 ttatatttga tttggtcacc ctcttatttc caattacatc tattttcttt acatcaaata
    29641 aatcactttt tacaattata catatacaaa aagataacat aagtataaaa actaaaacta
    29701 tcatgacatt attcgtattt aactttcttc tttttttcaa atataatcaa cccctttggt
    29761 taataaatac acctttagag cttgtttttc ctacaaaaaa attataatat ttaaagtcaa
    29821 atttttgcat tttattacat aacatccaaa taaggtatta aattttataa ataaatcaat
    29881 atttcatcaa aataaacata gcatactaca tactatttat atatttaaat ctgtatacat
    29941 acaaataaac ccctaattta taataattta gaaaatctaa tatattataa attaggggta
    30001 aatatttact tatcttttct ttattcctat taccttagct tgaccttcta tacttggagt
    30061 cgtttctttt tttatttctt ttacttcctt aataactttt tctttttttg tttttttaga
    30121 agctgatttt tgtgttgaat tgtatacttt cattatctca tcatatataa ggtctatggc
    30181 ttcaggctta cctatagttt tacttgcatt tgccatatca accaaaagtt ctctatctcc
    30241 taaaagttta aatactgctg tatttaggct ctctggagtt agattttttt ctaaaatagc
    30301 aattcctgcc ccttgttttt ctatactctt ggcattatac tcttgatgat tttcagcagt
    30361 ataagcttta ggtattatta tagatggctt tccaagagcc gttatctcag ctaatgaaat
    30421 tgctcctgcg ctacctataa ccaaatcact tgctgcaagt gcatttgcca tatcttctaa
    30481 gtaaggcaca actttttggt atggtttaag atttatatcg ctaatacttc ccataaattc
    30541 atcataatag cttttgcctg tagcaaatat aaaagctata tcttcattta ccatattctt
    30601 tattacaagt ctcattgcat catttatttt tcttgaacct ccactaccac catagcaaag
    30661 taccattctt ttttcgtcac ttattgatag attctttctt gcaatatttt ttcttgataa
    30721 taaaatttct tttctaactg gatttcctgt aaaaactaat ttatcttctg ctgcctctgg
    30781 aaatctttta tgcgaatctt caaaacttgt aagtaccttt gtaactgttt tagataaaat
    30841 cttattagtt acaccaggaa aagaattttg ctcatgtatt atggctggta ttttccccat
    30901 agatgcatta aataaaacag gtccactaac atatccacca gttcctataa ctatatctgg
    30961 tttaaatttc ttaacaatct ttcttgattg ctccaaacct ttaaaaagct taaaaactct
    31021 ttttacatta tcgaaatcta tttttcgctt aaaaccttga actgttacag tctttaattc
    31081 aaatccatac ttaggaacta tttctgactc tattcccttt tccgtcccaa caaaaattat
    31141 ctcagcgtct ggatgctcat ctcttatttt gtttgcaata gctatagctg gatacacgtg
    31201 ccctccagtt ccaccgcctg ataataaaac tttcatcaaa tcatcatctc ctgttaatta
    31261 attttgacat gctttgagat gtttaacact attcctacag ctcccataaa gatggtgagt
    31321 gaagttcctc cataacttat aaatggtagt gctacacctg tagctggcat tgatgatgtc
    31381 gcaactgcta tatttaaagc tgcttgtatt ccaatttgtg cacctatacc tataacaacc
    31441 atacaagcaa atacattaga agtcttcaaa gctattctta cacatctgta gactaaaact
    31501 acaaatagca taataactat tatacaacca atcaatccta actcttctcc tattatagca
    31561 aatataaagt cattttgtgg ctctggtata taaaaatact tttgtttact ctttcctaag
    31621 cctagaccaa acaatccacc tgaacctagt gcatatagac cttgtattac ctgatacccc
    31681 ttaccaagag ggtcttgaaa agggtctaaa aatgatgtaa cacgactaag tctatagggt
    31741 tcagcaatta ctaatgcagc aaataaagca gccccactcc ctatcatagc aaatacaatt
    31801 ttcatatcca ttccagcaac aaatatcatt acaaaagtaa ctagaattac agtacctgct
    31861 gttgacaaat ttggctgaag tattatcaac gcaaaaaata tccctggaac tacgagaagt
    31921 ggtacaacac cttttgtcaa agattttatt ttatcataat ttttttcaat taattttgca
    31981 gttaatatta ttgtagcaaa ctttgcgatt tctgcaggtt gaaatgttaa agctcctatt
    32041 cccaaccatc tttttgctcc gtttgcttct atccctaaag gggtcaaaac taacaataat
    32101 aatactactg ctatagctcc aattgctgta gcatttttct tccaaaaact ataatctatt
    32161 cttgatgtaa taatcataac tataaatcca agtacagcat atattacatt cttttttaga
    32221 aaatagtacg catcattgtg tttaaatgaa gattgtataa aacttgcact aaacaccata
    32281 actattccta caaatactaa aagcatagta gtgtaaaaca ctactccatc aaattcagtc
    32341 ttcatcctta tgtcaatttg ttttttcaaa ttttctttag gcatccaact aatctacctc
    32401 cattcttcca tttaagtaag tttagaggta agagattatt ttaaattgtt tacattatct
    32461 ttgaagtctt ttcctcttac ttcaaaactt tcatacatat cccaacttgc acaagctggt
    32521 gaaagaagca ctatatctcc actttttgaa atttcataag aagtcttaac tgcttcttcc
    32581 atatctttta ctatatgtat gtcattaaat cctttatttt tagcacaatt ttctatattt
    32641 gatgcagttt ctcctaataa gaccaatgat ttaacatatg actttgcagt ttcaagaagt
    32701 tcatcaaaat tacttccctt atccatcccc cctgctataa gtattatagg tctatcataa
    32761 gactgtatag cttttatagt ggaatctgga ttagttccct ttgaatcatt tacatatatt
    32821 acattgtcta aatttcttac aaactcttgt ctgtgttcca ctcctttaaa tgtcataagt
    32881 acatccttta taacttctaa atcaattttg cagacatatg ctattgctat agctgccata
    32941 caattttcta agttatgacc tccaggtaaa cttacatcat ctttatttag aaatttgatt
    33001 ttatcatcta tatctattat tatgtcatta ttttctaaat atactcctcc atttactttt
    33061 ttagttcttg aaaagtaaat tactttggca ttgcatttat cagctaaaga ttttactatc
    33121 tcatcatcat agtttaatac acagaaatcc tcaacagttt ggttcataaa tatatttgcc
    33181 tttgcttcta tgtatttttc catagtatgg tgtctattta aatggtcctc tgtaatattt
    33241 aatattgcgc ttactttagg tctaaattca tctatactct caagctgaaa actgcttagt
    33301 tcagttacta acacagactc ttcacttgaa gtttctatag tatctattac aggatttcct
    33361 atattaccaa ctacatatgt atctcgtttt gctcttgaaa aaatttctcc taccaaacta
    33421 gttgtagttg tctttccatt tgtacctgta atccctataa aaattggatt attcgatagt
    33481 ttaaaagcta attctacctc accaattata tacttgcctg aattttttaa tttcaatatg
    33541 aatggtaggt ctagtggtac acctggagac actacaacca tatctatatc atctacatct
    33601 tctgggtgat gtcctaatat gtattttatg tcatttatag atttcaattc atcaagtata
    33661 tttctcaatt tattttcatc ttttatatca tttacgatta tacttgcacc caatttatct
    33721 aaatgtttaa ttgtagatat accagttttt gcaagtccta ctaataaaac tttttttcct
    33781 tttaagtcca tttttcattc cccctagccc taaaatactg ctattatact tatccaagct
    33841 aaaacaactg ttataatcca aaaaataaat acaactcttg tttctggcca accacactgt
    33901 tcaaagtggt ggtgtattgg agccatctta aagaatctct ttcttgttgc cttaaaatat
    33961 ccaacttgaa tcaaaactga tagagcctcc gcaaaataaa ttcctccaat tataggtatt
    34021 ataagcactg agttagttag aactgaaaat gctacaactg cacctcctaa tgccattgaa
    34081 cctgtgtcgc ccataaacac tctagccgga tatgaattaa atcctaagaa acctaaacat
    34141 gctccaacag ttgctgctgc taaaactgcg acttctgtat ttccagcaat agaacttgca
    34201 aatagcataa agaatacaga aactattaat gttactcctg atgctaatcc atctaatcca
    34261 tcagtcaagt ttacagcatt tactattgct acgattataa acatcattat tggtatatat
    34321 aatattccaa catttatcac aaaatctgta aatggtatca taagttgtga agcacttgat
    34381 gatgatgtat actgataaaa agcaacataa aaagataatg caacttgaag gattattttt
    34441 tgatatgctt ttagtccaag tgatcttttt agctttattt ttataaaatc atctaaaaag
    34501 ccaataaaac caaaacctgc aatacatata agacctactg ccatatcatg actaactttt
    34561 actcttgtaa gtccagttat tagaattgca acaatcatta taattccacc catagtaggt
    34621 gttccgtttt tagctagatg tgtttggggt ccatcatctc taactgtctg accaaattta
    34681 aattttctaa gcattggtat aaatattggt cctattataa ttactattaa aaacgcaatc
    34741 aatgcggtgt acgtaagctc tgttattcct aacatcataa aactcctctc ccttgtcaat
    34801 taactcatta gtttaagctt atttatttag atattcgact attttttcta atctcatgcc
    34861 tcttgagcct tttactaaaa tagtgtctcc tgtttttact aattcattta acttgttaaa
    34921 cacatcttct ctattctcaa agtgatatat attggctgaa ttgaatccta attgttttgc
    34981 ttcttctcca ataaacactg aattttcacc tatagttatt ataatatcag tattattcat
    35041 agatgatttt ccaactaatc tatgaccata ctctgaaatt tcacccattt ctagtatatc
    35101 tccaagtatc gctacccttc tacctttata tcttccaagt atctttaaag cagcatccat
    35161 agaatctgga ctagcattat atgaatcatt aattattgtt aatttttcat tttttattat
    35221 gtctagtctc atttttgtcc ctttaaaatt ttttaaacct ttttttatat aatctaaaga
    35281 aatatttaga cacaaaccaa ctaatatagc agacattgca ttatatatat tatgttctcc
    35341 tattgttggt ataaatattt cctctttttt cccatttatt acacatataa atgttaaact
    35401 atcctcttcc atagtataac tttcacaata aacatcattg tttttattaa agccaaatgt
    35461 ttttaactta tatacatgtt ctttttcttt taatgtagat aaaaacttat catctccatt
    35521 tacaattaaa gtatttgttt catcaaagtt tgaagtaatc tccatctttg ctttcaaaat
    35581 accctcttgt gaacctagtt tttctacatg tgataatcct atattcgata tgactgcaat
    35641 tttaggattt actatatcca ctaaatattt tatttcattg aatccagaca tacccatctc
    35701 tattacagca cactcatgtt ctttatttag attaaataaa gtcaatggaa ctccaaaatg
    35761 attatttaag tttttttcat tttttagtat atttaacttt gcagatatgg cagagtaaat
    35821 catatctcta gtagtagttt ttcccacact acctgtaaca cctataaatg gaatatcaaa
    35881 tttttcttta tagtattttg aaatatctcc tagagccaaa gatgtatctt ttacctctat
    35941 taaatttata tccgaacttt ctaattttat tccattactt tcatttttta taaaagtttt
    36001 acatccacta tcatatgcag acttcataaa tgtatgccca tctaaatttt ctccaactat
    36061 ggctacaaat gcattttggg catttgcctc cctgctatct ataactatat cacttacaca
    36121 gtcattataa tctccatata ttaacttccc attagttgca agtactaact cttttattgt
    36181 taagcactcc attattaaac tcctccttta aaaccttagc tatattctct tagcaacaaa
    36241 aggcaatgta taccgttata tatacattgc ctacgataac tcttaacttt ataattatat
    36301 actatatttc tgtttttttg aagatgttta tgggtatatg ttaattatct gttacttttt
    36361 agttttcaaa atatagggtt atacttgagt cttcattaac ttttactcca ggttttggga
    36421 atatatcctt tacctttgta tcttctggaa gttctatatc tgcatctaag tctggtttta
    36481 acttcgcatc ctccaatgcc tttacagcat ctcctatttt tagatttctt acatctggca
    36541 ctttaacttg cttcttttca tactcagctt tttcttcttc tttatatact ggtttaactc
    36601 ccaaatattt taaagagtca ttcattattt ctttcactat tggtcctgct gtagtactac
    36661 caaatgcact tactcctgtt ggttcgtcaa caatagcaag aactactatt tgcgggtcat
    36721 cacaaggtgc tattccaaca aatgaacata tatattttcc tggagcatac ttaccatcta
    36781 taactttttg agctgtacct gttttaccac caagtctata acctggtata tatgctatct
    36841 ttcctccacc ttcagttaca actgattctg caatttctaa cattttttta gatgtttctt
    36901 tagatataac acttcttact ttttttggtt ttactgtttc agttatattt cctttattgt
    36961 cagtataaga ttttaccact cttggttgca ttaaatctcc accattagct attgatgata
    37021 tagctgttat aagttgtatt ggagttactg atatagattg accaaatgat attgtagcca
    37081 attcaactgg tcctacattt ttttcgttat acagtattcc cttagcttct cctggtaaat
    37141 ctatacctgt cttatccata agaccaaatg attctatata atcatacatt tttccaacac
    37201 ctaatctgct tcctaactca acaaatactg ggttgcaaga gttttgtact gcttgtttaa
    37261 attcttgtgt tccatgaggt ctgtaatgtc tccaacattt aatctttctt cctccaacag
    37321 ttacactacc tgtacatgtg aatttttctc catctttaat tactccttct tcaagagcac
    37381 tggaagatgt aataagctta aatgtagaac ctggttcata tgtatcactt actgctggat
    37441 ttctccacat ttgataatag ccttttattt tatctttatc attatatttt tcaagttctt
    37501 cttgataata tggatatatt ggcgttcttg agtcattagg gtcataatct ggttttgatg
    37561 ctaaagcaag tatatcacct gttttagggt tcatagctat tgcagtaacc ttttttgcat
    37621 tatttaactc ataagctttc tgtactgctt tttcagtata gtgttgaatt acttcatcta
    37681 tagacaacac aagaccatta ccttgtacag gctcataata tttttccatt ccttgtggta
    37741 tttctcttcc tgaagcatca gtacttacta taagttttcc aggctttcct tttaactttt
    37801 tatcatattg catttctaca ccagatatac ctgttgcatc tgaagaagta tgtcctaaca
    37861 catatggtgc aaaattccca tatgggtaat atctttgatt atcttctgcc acccatattc
    37921 cacttaattt agcatctctt atttgacttg ccttatcatc atctatccat ctttttactt
    37981 ttacaagagc catatttttt ttgcttatta aagcgtaaat atctttataa tcttcatcca
    38041 aaatctcagc taccttttcg gctgcttctt ttttatcttc tacttccaca ggtttacacc
    38101 aaacagtata cttagttaca cttactgcta actctttcat atttctatca tatatagttc
    38161 ctcttttggg ttctattggt atatctcttg tttgttgttc tagtgctttt gtactcaacc
    38221 aatttccctt cataagttgt agatacccag tcctaataac aaggcaaaaa aacaatgcac
    38281 atgctagaat aagaacgagt accagccttt tcttacttat cctctttact tttctcaaat
    38341 ctattccccc tagttatcca catctatata ctttatttgt ccatcttttg gatagtccat
    38401 attgagcaat tccttagcct ttagctcaat ttcttttatt gatgtttttt cagtttcttt
    38461 tgcctttagt tcctctaata ctatctcttt ttttcttaaa tctttttgta aataatagat
    38521 atcgtacttc atttccgaga tttttgaata accacataga ttcaatatta ttactgaaaa
    38581 agttgcaatc agtaaaatgc atttagctcc tatttttttc ttatttctct ttttataatt
    38641 attctttttt ttatttttat attcatttag attatgtatt ttataatttt ttttcaaatt
    38701 ctcaccccga tagtatatag tttaaatttt cctctatact atatttgtta ttaatcatat
    38761 ttcttcctct aaaatttcat cgctaaatta atacactatt acacaatcta attgtatttt
    38821 tttctataca caacaaaaac ctataaattc acttatactt ttatatgaat ctataggtta
    38881 gatattaatt tctatttatt tttatcactt ctagtatttt tagataattt cttccataaa
    38941 tataatatgt aactagctat ctactctctg attcctaagt tctccattat attacttaat
    39001 accatctata tctatatttt tatatctaaa ttttcattat atatctcaat aacaaagaac
    39061 atttcttata gtgttaattg ttttttaatt gctttaccag tgcaacaaaa tatataatac
    39121 tttttaatta attataataa tcttatatat aagattattc tttagcctta taactactta
    39181 tattttttct gctactctta gcttagcact tcttgaacgt ggatttactt ctatttcttc
    39241 ctcactaggt aatataggtt ttctagttat tattttcacc tcagattctt tatcacattg
    39301 gcatataggt aaatgttgtg gacatataca atctgatgct aaatgcttaa atgcattttt
    39361 cactattcta tcttctaatg aatggaatgt tattatacat attcttccac cttcattcat
    39421 tatagaagaa gcatcattta tcatttttgt aattactcca agttcattat ttacttctat
    39481 tcgtattgcc tgaaaagttc ttttagctgg gtgaggtcca tctattctag ctttcttagg
    39541 gatagctttt ttaattacat ctactaattc tcctgtagtt tctattggtt tgtttgctct
    39601 ttcttcaact ataaatttag ctatacgctt agcccagtta tcttctccat aatcttttat
    39661 tatcttagtt aattcatctt ctgtataagt atttacaaca tcatatgcag aaaactcaca
    39721 cctaacatcc attctcatat caagtggtgc atcttgcata taagaaaatc ctctatcagc
    39781 ttcatcaagt tgatgagatg aaactcctaa atcagctaat acaccatcaa ttttgtaaac
    39841 tcctattttt tctaattcct cttttatatt ttcaaaattg ctgtgaacaa acttcactct
    39901 actttcatat tcactcaatc tctcttttgc tgtgcttata gcatttttat cttggtcaaa
    39961 ccctataaat aatcccttat ctgacagttt ttttactatt tctttagaat gtcctgctcc
    40021 tcccatagta cagtcaacat acacgccatc tggctttata tttaaattct ctatacactc
    40081 atttaaaaga actgatacat gatgaaattc catagttacc tcccttaaat tcacttttta
    40141 aactattctt cactaatatt atttttattt ttctttgata aataaacatg tgcaattatg
    40201 ccaccaataa ctccaattag acttataact tgtgccattc taagtggtcc taacattaaa
    40261 ctatctgttc ttaatccctc tataaaaaat cttcctattg aatataaagt tatataagta
    40321 actataactt gaccttcata ttttttattt tttctaaata gtaataaaac tataaatatt
    40381 ccaaaatccc atatagactc atataaaaaa gttggatgta cctttactcc atcaaccatt
    40441 attccccaag gtaaattggt agcacctcca tgtgcttctc cattaataaa atttccccat
    40501 cttccaatag cttgtgctaa aggcattcca agtataactg tatcagccat ttttaagaag
    40561 ttaatatttt taatcttagt gtatatataa ccagctaata ttccaccaat taaccctcca
    40621 tgtatagcaa gtccaccacc tctaaaatta aaaatttgag acatattttg agcatagtaa
    40681 ctccaattaa atacaacata atagattctt gcacagatta gtccaactgg aattgctatt
    40741 atagctatat ttaatacatc atcttcttta attcccactc tttttgcttc ttttattgct
    40801 attaaagttc ctaaaatcat tccacatgcc ataagtattc cataccacat gatgtctatg
    40861 ccaaacagtg taaatgctac tctatccata acgtcacctc tttgtattaa ttgaaaatat
    40921 ttccaaagca atatgctata attacaatta attgtaatta tactaaatta tgataacaca
    40981 ttaatgttaa ttctacaaaa aatattaaaa tttattctac aactccctac actatattat
    41041 atctaattac tataaaattt tttaaactga atctctcatg actaaagtta caagtgtaca
    41101 tagatatttt ttaatcaaaa agattgatgc atatatacat caacctcttc tattccttat
    41161 ctatagctct taaattttta cctcaattta tataaatata ttttataaat ttgttttacc
    41221 aaaataaaca tttaaaaaca taaactaata cttaattagt tattcttatt atgattaagt
    41281 ttctatttag gctctacaat agaaataaca caatactctt ctttaaaatg ctcttttaat
    41341 ctttcttcaa cttcctcaaa agtaatatct ttaataacat ctaaataatc caatagattt
    41401 atatccttaa aaacataaga tataaaacta ttacctataa agtttataga atcaaagcac
    41461 ttaataaatg accctatttt tttcttttta gttctctcaa actcttcttt tgataatcct
    41521 tctttttttg atttttctat ataatcaaga attatttctt ttactttttt aggctcttta
    41581 gaatctccag ctattatgct aaaagcataa tccacttgag acgaaaatcc agctccaaaa
    41641 ttttcattta ttaaaccttg catatacaaa tcttcataaa gcttacttcc ttttttaaat
    41701 aacatcccta caagtatatc agttacaatc tctttacgta aaagttcttt cccttttaaa
    41761 ccaacattgc tatctttaaa acctatgttg aacataggca tagatatagg gaatttttct
    41821 ataacctctt tttcttttac actttctggt tcttctggat aaaatctttc tatactctta
    41881 gagagtctgt ctactttgta attgtttgac tttttagtta catctataac tttttctaca
    41941 tctaagtcac caacaacaaa taaagccata tttcctggat tatagaaagt attataacat
    42001 ttatatagct cttccttagt tatcttatat atactatcaa cagtacctgc tatatcaatt
    42061 ctagcaggat aatttacata cattgccttc aagcaattaa agtatacatt ccaatcaggg
    42121 tcatcattgt acatttttat ctcttgtgct attatacctt tttccttttc aacattttca
    42181 tctgtaaaat aaggtgtttg aacatagtca attagatgtt ctaaactctc ataaaaattt
    42241 tcagtagcag aaaataagta ggctgtcatt gtaaaattag taaatgcatt tgcatttact
    42301 cctaattttg aaaatttatc aaatgcatct cctccatcag gttgctcaaa catcttatgc
    42361 tctagaaaat gtgcaatacc ttcattcact ctaattttct tatcttctcc aattggtaca
    42421 aattctaagt catttgaacc ataattagta gccaaaatag catacttttt cataaaaccc
    42481 cttttaggca taaaatatac atctaatcca ttttgcaatt tctcataata aacttcttct
    42541 ttcaatatat cattaactat tttttccata gacatctcct ccattaattt ctcaagaagt
    42601 atacagtatc aagctctata ttttttatag cttcaactat atcttttttt gtaacctttt
    42661 ctatactaga tattatgtct tgtacttcag aatttgtctt tgccatagac tgagagaaaa
    42721 caaaatcaga cataccacct atattatcag ttatagattt catagaatta actaaagcaa
    42781 gtttactgtt ttctaattct tcatcagata tatcacctgc ttttacactt tctacttgtt
    42841 tttttatcaa ttctacagct ttatcataat ctttagtttc tataccagat gatataaaca
    42901 tgatactttt atacttctct attgaagaga atatatagta acataaactt tctttttctc
    42961 ttacattaac aaataattta gagtgtggac caccacctaa gacattgctc ccaactacta
    43021 aagcataata tttatctatg tctgcataat ccacatttgc tctataaccc ataactaact
    43081 taccttgagt tatttccatt tgttcatcta taacttttac ctcatccaca ttttttataa
    43141 aatctgctct tggtatattt ataatttctt ctctttcaaa ttttagattt ttacttatta
    43201 tatctacaac tttatcttca tcaaaattac cttctacaac tatatctata ggtgatgttt
    43261 taagtatgtt tttataatgt tcatataact ctcttgaagt tatactatct atttcatctt
    43321 cataaccaaa ttcacttaca ctatatctct ctcctttaca catagcctca atacatttat
    43381 cttgcgcata ttccttttta tcatttatta ttgattgtat cttttctctt aaattctctt
    43441 tttcaatatt taaatattct tctttaaacc caccatctac aacaagcgta ttatttatta
    43501 cttcattaaa aaattctact acttcttcaa atatactctc atctaaatac ttttcactta
    43561 tgttaattac tttaaaactc aatacttgac gttctccacg ttttacagca tcagctccca
    43621 tagatgaacc atataaatca tctaatttat tggatattgc tctagcacta ggatatttag
    43681 cgcttccact tgctattatt gatggtatta gagcattttt tgtagtttct ttttcatcca
    43741 atagtctctg aacatataaa cttataagat tcgatttaaa cttttctgtt tttatcaaag
    43801 taagattaat attattacct aaatttatta tttttttagt attaaccata tgtacctcct
    43861 atttttattt aaacatcaat tttaaaattg aaatttattt attttttaat tacatatact
    43921 cacatataaa aaccttctat ttaattatat cctttttatt attttctaaa agcaatattt
    43981 ttatttaaat taaatacaaa tattatttaa atcaatatgc tttaaagtct agtttacaag
    44041 cttgatatat atgatgtata atcaacatgt atgtctataa ttaaaacaat tttgcaatcc
    44101 tatagattga ttgtttaaag ttatataaag aatataaaaa agaataagag agaggacgat
    44161 taatatgaaa ttaggtatag ttggtttacc aaatgtaggt aaaagcacac tatttaacgc
    44221 aataactcaa gcaggtgcag aatctgcaaa ttaccctttt tgtacaatag acccaaatgt
    44281 gggagttgta tctgttccag atgaaagatt aaacaaacta caagaattat ataattctga
    44341 aaaaatagtt cctactgcta tagaattttg tgatatagca ggtttagtta gaggagcttc
    44401 taaaggagaa ggtttaggaa ataaattctt atcacatata agagaagttg atgcaatagt
    44461 tcatgttgtt agatgttttg aggatgaaaa tgttgtacat gtagatggtt ctgtagaccc
    44521 acttcgtgat atagaaacta taaacttaga attaattttc tcagatatag agattcttga
    44581 aagaagaatt aataaaactc aaaaagctgc taaggctgat aaaactttag gttctgaact
    44641 tgatttatta aaatcaataa tgtctacttt agaagaaagt aagtgtgtaa gaactatgga
    44701 atttacagaa gatgaacaaa cattcgtaaa ttcattggat ttacttacat ctaagcctgt
    44761 aatatatgct tcaaatgtat cagaagatga tttagctgat aatggagaaa ataataaata
    44821 tgttcaacaa gttaaagcat ttgcagaaac agaagatgcc gaagttgttg tagtatgtgc
    44881 acaaatagaa gcagaaattt ctgaacttga ttctgctgaa gaaaagaaag agtttttaga
    44941 aacattaggt cttgaacaat cagggcttga taagcttata aaatcttctt atgctttact
    45001 tggtttaatt tcattcttaa ctgctggtcc aaaagaagtt agagcatgga ctataaaagt
    45061 tggaagtaaa gctcctcagg caggaggaaa aatacattct gatatagaaa gaggatttat
    45121 cagagcagaa acaatagcct ttaatgattt ggttgagcat ggtactatgg ctgctgctaa
    45181 agaaaaaggg ttggttaaat tagaaggtaa agaatacata gttaaagatg gagatgtaat
    45241 attatttaga tttaatgttt aaaatgatta atcgtataca ctttgactat ta
//
LOCUS       DAELNY010000011        44248 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.011, whole genome shotgun sequence.
ACCESSION   DAELNY010000011 DAELNY010000000
VERSION     DAELNY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.011"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            17..1213
                     /locus_tag="KPE55_000520"
     CDS             17..1213
                     /locus_tag="KPE55_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG0491621.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1306..1830
                     /locus_tag="KPE55_000521"
     CDS             1306..1830
                     /locus_tag="KPE55_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG0491622.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1855..2031
                     /gene="rpmF"
                     /locus_tag="KPE55_000522"
     CDS             1855..2031
                     /gene="rpmF"
                     /locus_tag="KPE55_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG0491623.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2238..2795
                     /gene="fapR"
                     /locus_tag="KPE55_000523"
     CDS             2238..2795
                     /gene="fapR"
                     /locus_tag="KPE55_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG0491624.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2806..3828
                     /gene="plsX"
                     /locus_tag="KPE55_000524"
     CDS             2806..3828
                     /gene="plsX"
                     /locus_tag="KPE55_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG0491625.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3854..4840
                     /locus_tag="KPE55_000525"
     CDS             3854..4840
                     /locus_tag="KPE55_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG0491626.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4949..5878
                     /gene="fabK"
                     /locus_tag="KPE55_000526"
     CDS             4949..5878
                     /gene="fabK"
                     /locus_tag="KPE55_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG0491627.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5894..6844
                     /gene="fabD"
                     /locus_tag="KPE55_000527"
     CDS             5894..6844
                     /gene="fabD"
                     /locus_tag="KPE55_000527"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG0491628.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6844..7593
                     /gene="fabG"
                     /locus_tag="KPE55_000528"
     CDS             6844..7593
                     /gene="fabG"
                     /locus_tag="KPE55_000528"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG0491629.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7647..7871
                     /gene="acpP"
                     /locus_tag="KPE55_000529"
     CDS             7647..7871
                     /gene="acpP"
                     /locus_tag="KPE55_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0491630.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7900..9138
                     /gene="fabF"
                     /locus_tag="KPE55_000530"
     CDS             7900..9138
                     /gene="fabF"
                     /locus_tag="KPE55_000530"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG0491631.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9364..11082
                     /locus_tag="KPE55_000531"
     CDS             9364..11082
                     /locus_tag="KPE55_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0491632.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11113..13113
                     /locus_tag="KPE55_000532"
     CDS             11113..13113
                     /locus_tag="KPE55_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0491633.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13464..13709
                     /locus_tag="KPE55_000533"
     CDS             13464..13709
                     /locus_tag="KPE55_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0491634.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13744..14469
                     /locus_tag="KPE55_000534"
     CDS             13744..14469
                     /locus_tag="KPE55_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0491635.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14491..15804
                     /locus_tag="KPE55_000535"
     CDS             14491..15804
                     /locus_tag="KPE55_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0491636.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVYLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16010..16549
                     /locus_tag="KPE55_000536"
     CDS             16010..16549
                     /locus_tag="KPE55_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0491637.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16638..18623
                     /locus_tag="KPE55_000537"
     CDS             16638..18623
                     /locus_tag="KPE55_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG0491638.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18847..19815
                     /gene="spoIIIAA"
                     /locus_tag="KPE55_000538"
     CDS             18847..19815
                     /gene="spoIIIAA"
                     /locus_tag="KPE55_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG0491639.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19805..20326
                     /locus_tag="KPE55_000539"
     CDS             19805..20326
                     /locus_tag="KPE55_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG0491640.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20343..20540
                     /gene="spoIIIAC"
                     /locus_tag="KPE55_000540"
     CDS             20343..20540
                     /gene="spoIIIAC"
                     /locus_tag="KPE55_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG0491641.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20556..20942
                     /gene="spoIIIAD"
                     /locus_tag="KPE55_000541"
     CDS             20556..20942
                     /gene="spoIIIAD"
                     /locus_tag="KPE55_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG0491642.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21027..22232
                     /gene="spoIIIAE"
                     /locus_tag="KPE55_000542"
     CDS             21027..22232
                     /gene="spoIIIAE"
                     /locus_tag="KPE55_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG0491643.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22292..22909
                     /locus_tag="KPE55_000543"
     CDS             22292..22909
                     /locus_tag="KPE55_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG0491644.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22921..23475
                     /locus_tag="KPE55_000544"
     CDS             22921..23475
                     /locus_tag="KPE55_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG0491645.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23502..24191
                     /locus_tag="KPE55_000545"
     CDS             23502..24191
                     /locus_tag="KPE55_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG0491646.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24301..24663
                     /locus_tag="KPE55_000546"
     CDS             24301..24663
                     /locus_tag="KPE55_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0491647.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24748..25254
                     /gene="nusB"
                     /locus_tag="KPE55_000547"
     CDS             24748..25254
                     /gene="nusB"
                     /locus_tag="KPE55_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG0491648.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25238..26308
                     /locus_tag="KPE55_000548"
     CDS             25238..26308
                     /locus_tag="KPE55_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG0491649.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGYNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSVKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26305..27510
                     /locus_tag="KPE55_000549"
     CDS             26305..27510
                     /locus_tag="KPE55_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG0491650.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27512..27727
                     /gene="xseB"
                     /locus_tag="KPE55_000550"
     CDS             27512..27727
                     /gene="xseB"
                     /locus_tag="KPE55_000550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG0491651.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27730..28617
                     /locus_tag="KPE55_000551"
     CDS             27730..28617
                     /locus_tag="KPE55_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG0491652.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28647..29084
                     /locus_tag="KPE55_000552"
     CDS             28647..29084
                     /locus_tag="KPE55_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG0491653.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29118..30983
                     /locus_tag="KPE55_000553"
     CDS             29118..30983
                     /locus_tag="KPE55_000553"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG0491654.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31116..31928
                     /locus_tag="KPE55_000554"
     CDS             31116..31928
                     /locus_tag="KPE55_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG0491655.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31948..33627
                     /gene="recN"
                     /locus_tag="KPE55_000555"
     CDS             31948..33627
                     /gene="recN"
                     /locus_tag="KPE55_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG0491656.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33841..34707
                     /locus_tag="KPE55_000556"
     CDS             33841..34707
                     /locus_tag="KPE55_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491657.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34800..35291)
                     /locus_tag="KPE55_000557"
     CDS             complement(34800..35291)
                     /locus_tag="KPE55_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0491658.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35352..35792)
                     /locus_tag="KPE55_000558"
     CDS             complement(35352..35792)
                     /locus_tag="KPE55_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0491659.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35953..37011
                     /locus_tag="KPE55_000559"
     CDS             35953..37011
                     /locus_tag="KPE55_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0491660.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37153..37977
                     /gene="spo0A"
                     /locus_tag="KPE55_000560"
     CDS             37153..37977
                     /gene="spo0A"
                     /locus_tag="KPE55_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG0491661.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38390..39511
                     /locus_tag="KPE55_000561"
     CDS             38390..39511
                     /locus_tag="KPE55_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491662.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39529..40404
                     /locus_tag="KPE55_000562"
     CDS             39529..40404
                     /locus_tag="KPE55_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG0491663.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSAKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40428..41120
                     /locus_tag="KPE55_000563"
     CDS             40428..41120
                     /locus_tag="KPE55_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0491664.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41128..41952
                     /locus_tag="KPE55_000564"
     CDS             41128..41952
                     /locus_tag="KPE55_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0491665.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41957..43024
                     /locus_tag="KPE55_000565"
     CDS             41957..43024
                     /locus_tag="KPE55_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG0491666.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43026..43562
                     /locus_tag="KPE55_000566"
     CDS             43026..43562
                     /locus_tag="KPE55_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0491667.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43630..44235
                     /locus_tag="KPE55_000567"
     CDS             43630..44235
                     /locus_tag="KPE55_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG0491668.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
BASE COUNT        17922 a         4452 c         8288 g        13586 t
ORIGIN      
        1 taaggaggaa ataataatga aaatattagt attaaactgt gggagttctt cattaaaata
       61 tcaattaata gatatgaata atgaagaagt tttatgtata ggattggttg aaagaatagg
      121 aatagaaggt tctatattaa aacatgaaaa agcaggtaga gatgataaat acgttgttga
      181 acaaccaatg aaggaccaca aagatgcaat agcattagtt ttagaagctg ttgctcatcc
      241 agaatttggt gcagttaaag aaatgaaaga aatagatgca gtaggacata gggttgtaca
      301 tgctggagaa aaatttgcaa cttcagtagt aataactcca gaagtagaag aagctttaaa
      361 agaatgtata gatttagctc cacttcataa tccagcaaac ataatgggaa tagatgcttg
      421 taaagctata cttccagatg taccaatggt aggtgtattt gatactgctt tccatcaaac
      481 aatgcctaaa tcttcatact tatatggttt acctcatgag ctatatacta aatatggggt
      541 aagaagatat ggattccacg gaacttctca taattatgta tctcaaagag cagcagagat
      601 attaggaaaa gatataaaag atttaaaaat agtaacttgt cacttaggaa atggtgcttc
      661 tatagctgct gttgatggtg gaaaatgtgt agatacatct atgggattca ctccattaga
      721 aggattaata atgggaacta gatgtggaga tatagaccca gctatattac cattcttaat
      781 gagaaaagaa ggtttagatg ctgatggatt ggataaatta atgaataaag aatctggagt
      841 atatggaatg actggaattt ctagtgactt cagagatata gaagatgcag ctaaaaatgg
      901 agacgaaaga gctcaagcta cattagaagc gtatgttaaa aaagtacaaa aatatatagg
      961 cgcttatgct gctgaaatga atggattgga tgttgtagta tttactgcag gcgttggtga
     1021 aaatggaaag gctataagag cagatattgc ttctaacatg gagtttttag gaatgaaatt
     1081 agataaagaa gctaatgatg ttagaggaaa agaaacagta atatctactg ctgattctaa
     1141 agtaaaaatg cttttaatac caactaatga agaattaatg atagctagag atacattaag
     1201 attggtaaaa taattaaatt aaacctatgc ctatcaagta aaaatacttg acaaacaaat
     1261 gctagatttg tataataaat ttggtgatat gttgaggtga acttaatgaa agttagcata
     1321 gaaaaaataa atagaaaaga aactgacaaa atagacttga atttttgtga aaaaattgat
     1381 actattagtt attgtgatga gatttacaaa ttagtatcac ctgtaaattt aaaagggaaa
     1441 gtgtcaaaga ctaataaagg tttgtatcta gatatagatg tcaattttac aattgttgac
     1501 aattgttcaa gatgtcttaa agaagtagaa ataccactag agtattctat acaaggtttt
     1561 ttagtaaaag aagaagatta tgatgaagat gagtttgagg aatttgatcc ttttatattc
     1621 gatggtgaag aaatcgacct tattgatata atagaacaga cattagattt taatgtacct
     1681 cataaagttc tttgtagtga aaactgtaaa ggtctttgtc aagtatgcgg ggcaaactta
     1741 aatgaagaag agtgttcttg cagtgaaatt acaaacgatg aggaatacat agatcctcgt
     1801 tttgctaaat taaaagattt atttaattaa attttgacta aggaggtggc gtaaatggca
     1861 gtaccaaagc gtaaaacgtc taaatcaaac acaaaaatga gaagagcagc taactcaaag
     1921 atggaagcaa caggatttgt aagttgccca caatgtcatg agccaaaatt accacataga
     1981 gtgtgtccag actgtggata ttataaaggt aaagaagttg tgtctaagta atagcttcta
     2041 aaaagcatgt agagtatact ctacatgttt tttttgtaca tataaaaaaa taagataatt
     2101 tactagagaa aataagtgga ttttatgcat attaaaaaat agaaaaataa taatataagt
     2161 attgaaaatt tttgtgtgtt actatatact actattagta gctggtacta attacagcta
     2221 taaaatttgg agggaacatg aaaaagaaaa gtaaagccca aagacaaaaa gagcttatag
     2281 atatgctaaa gacagatcct ttttatactg atgaagaatt atcaagtctg tttgatgtaa
     2341 gtatacagac cataagatta gatagaatga gtctaaacat accagagcta agagaaagag
     2401 tcaaatcaat agcagaaact cagagttcaa aagtaaagac tttaggagta aaggaaatta
     2461 caggagaaat cattgattta tctgtagggc gattaggtat atctatgtta gaagttacac
     2521 aggacatgat ttactctaag actaatactt taaaagatac gtatattttt tctctagcag
     2581 attcattggc aatggctata atagatgcac ctaaggttat tatgagagtt gcaaatgtaa
     2641 aaagttttaa gttgatagaa caacaagata ggcttatagc aaaagcagaa gtgtatagaa
     2701 atatagataa aaagcattat gtaaaagttg ttatcaacaa taaagcccaa gagcaaatat
     2761 ttagaggaaa gtttatcttt gaagaattag attaggaggg aaattatgaa aatagtaatt
     2821 gatggaatgg gtggagataa tgcacctaaa tctaatgtag aaggcgcagt aaatgctata
     2881 aaagaatatc aggttgattt aataatcaca ggagataaag atttacttga aaaggaattt
     2941 tcaaattatg aatttgatag aaataaatta gagatagttc atactactga gattatagaa
     3001 aacgaagata aaccagtaaa ggctataaga agtaaaaaag attcatctat ggtagtagca
     3061 ttaaatcttg taaaagaagg aaaggcagat gctataatat cagctggaaa tacaggagcc
     3121 ttacttgctg gtggattatt tgttgttggt agaataaaag gaatcgatag accttgctta
     3181 tgttcagcaa taccaaatgt aaagcgagga atgacactta tagcagactg tggcgcaaat
     3241 gcagattgta aacctaagaa tctagttgaa tttgcagcta tgagtaatat ttattcaaga
     3301 aaagttttag gtcttgaaaa tcctaaggta gcattagcaa atgttggact agaagaaggc
     3361 aaaggcaatg atttagtcaa aaggtcatat gaagaaatta aaaaattaga tttaaatttt
     3421 atagggaatg tagaagctag agaagttata aatgcataca cagatataat catatgtgat
     3481 ggatttacag gtaatatatt acttaaatct gctgaaggtg tagcactttc agttatgagt
     3541 cttataaagg aaacttttat ggccagtaca aaaagtaaaa taggagcatt acttataaag
     3601 gatgatttaa gaaaattaaa aagttttata gattattctg agtatggggg agctccactt
     3661 ttaggtctaa atggaggagt tataaaagct catggtagtt ctgatgctaa agctataaaa
     3721 aatgctataa atcaaggaat aaaattttca aagggaaaag tcgttgaaga tattaaccaa
     3781 tttataagca aatacaatga agaaaataaa aataacgaag atgaataaac tcgttttgga
     3841 ggtttaatat ataatgaata caaaggctgg aatattagga gtaggaagtt atctacctga
     3901 gcaatcatat gataattttc acttcgaaaa aataatggat acatcagatg agtggataag
     3961 tactagaact ggtattaagg aaagaagatt tgccaaagag tctgaagcaa cttctgactt
     4021 agcatctaaa gctgccttaa aagctataga atgtgcaaag ttaaacgtag aggatataga
     4081 gctgattata ttagctacaa ttactcctga tatgtctttg ccttcaactg catgtatagt
     4141 acaagatgca ataggagcag tgaatgctac tgcatttgat atatcagcag cttgttctgg
     4201 atttgtatat ggagtaacta ttgcaaagca atttgtggaa acaggttgct ataaaaatgt
     4261 gcttgtaata ggagcagaaa cttgttctaa gtttttaaat tatgatgata gaacaacagc
     4321 agtattgttt ggagatggag ctggagcagc tgttattggc cctgtaaatg agggtggaat
     4381 actgtctaca catatgggct ctgatggtaa aggaaaagat tgtttaaaag tacctgctgg
     4441 aggttcaaga cttaaggcaa gtaaagagac tgtagaagct aatctacata caatagaaat
     4501 ggcaggtagt gatgtgttta aatttgcagt tagaaagatg gcagaaactt ctttaagagc
     4561 tcttgaaaaa gcaaatctaa atacaactga tatagactac ttagtgccac atcaagctaa
     4621 tataagaatc atacaagctt cttctaagag attagagctt gatatgaaaa aagtttatgt
     4681 aaatatagac aaatatggaa atatgtctgc tgcatctata ccagtagcat tagatgaagc
     4741 ttatagagaa ggtaaaatta aaaagggtga taatgtagtt ttagttggct ttggtggagg
     4801 tctaacttgg ggggcttcag ttgtcaagtg gactttatag tataaaatat ctaaaatgat
     4861 ttaattaaca aaattaaact tatacacaaa ataaagttga ttaataataa aaagtttact
     4921 tttatgattt caagtgaagg agaattgtat gaataaaatt tgcaaaatat taaatataaa
     4981 atacccagtt atccaagggg gaatggcatg ggtagctact gcatcattag caagtgctgt
     5041 atctaatgca ggaggacttg gcataatagc agcaggaaac gcaccaaaag aagctataaa
     5101 gaaagaaatt gttgagtgta aaaaattaac agataaacct tttggagtaa atgtaatgct
     5161 tatgtcgcca tttgttgatg atataattga tttgattata gaagaaaaag ttcaagttat
     5221 tactactggt gctggaaatc ctgcaaagta tatggataga ttaaaggaag ctggaacaaa
     5281 ggttattcct gtagtaccta caatagcttt ggcacaaaga atggaaaagc taggagctac
     5341 agcagtaata gcagaaggta ctgaaggtgg aggacatata ggagaactta ctactatggt
     5401 cttagttcca caagttgctg atgctgtaaa catacctgta atagctgctg gaggaattgt
     5461 agatggtaga ggaattgcag catcatttgc attaggtgcc agtgcagttc aagtaggaac
     5521 tagatttatt tgtagtgaag agtgttctgt ccattcaaac tataaaaact tagtactaaa
     5581 agcaaaagat agagatgcaa ttgtaacagg aagaagtact ggtcatccag taagaacatt
     5641 aaaaaataaa ctatcaaaag aatttttaaa gatggaacaa aatggagcta ctcctgaaga
     5701 actggataaa aaaggtacag gagctttaag atttgcaaca gtagatggag acatagaaaa
     5761 aggttcattt atggcaggtc aaagtgctgc tatggtaaaa gaaataacac cttgtaagga
     5821 aattatagag gctatggtaa atcaagcaag agagattatg ccagcaatag aactgtaaat
     5881 caggaggagc aagatgggaa aagttgcact agtattccct ggtcaaggag ctcaatatgt
     5941 aggtatggcc aaggatttat atgaaaataa tactgttgct aaaagtgtaa tagatgaggc
     6001 tagtgatgcc ttaaatatgg atttaaaaaa ccttatgttt aatggaaatg aagaagaact
     6061 ttctaaaact gaaaacactc aacctgctat ggttacacat agtgtagcag tattaaaagc
     6121 ggtacaagct caaatagatt taaaatataa tgcatgctta ggattatcct taggagagta
     6181 tagtgcgttg gttgcagcag atgcaattga ctttaaagat gctgtatgtt tagttaaaaa
     6241 aagaggtaag tttatgcagg aaacagttcc agcaggtgtt ggagctatgg cagcaatttt
     6301 aggtttagat agaaatgtac ttgaaaatgt ggttaaagat atacaaggag gtattgtaga
     6361 agtagcaaat tacaattctc ctggacaaat agttatatct ggtgaaaatg aaaagataga
     6421 agaagcaatg gtaaaatgca aagaagcagg agcaaaaagg gcagttaaat taaatgtaag
     6481 tggacctttt cactcatcaa tgttaaaaga agctggtgta aaattggcca gtgaattaga
     6541 aaaagttaac ataactaaac ctaaagttga tgtggtggca aatgtaaatg cagattacta
     6601 taaaaacgaa ggtaaagacc tgcttataaa acaagtaagt tcatctgttc tttgggaaga
     6661 ttctatagaa agacttttaa atgatggata tgatactttt atagaaatgg gacctggaaa
     6721 aactttaaaa gcatttatta agaagatagc ttccaataaa aaagcaaatg tcaatattta
     6781 taatatagat ggtatagatt ctcttaatga atttgtacaa aattatagaa atggagagat
     6841 ataatgataa atcttacagg acaagtagca gttgttactg gtggctctag gggtatagga
     6901 aaagaaatag caaaaaaact agcatctttt ggagctgatg tagtaatcaa ttatacttct
     6961 aaagaagatg aagcactaaa aactaaaaat gaaatagaaa gtatgggggt aaagtgtacc
     7021 tctataaaat gtgatgtatc taaatttgat gaagtgaatc aaatgataga ttctgttgta
     7081 agcgaatttg gaaaaattga tatattggtt aataatgcag gcataactaa agatggtctg
     7141 cttatgagaa tgaaagaaga agattttgat agagttatag atataaactt aaaaggtgtc
     7201 tttaattgta caaaagcagt tactaaacct atgatgaaaa agaagtatgg aagaataata
     7261 aatatgactt cagtagttgg aattatgggt aatgcagggc aaactaatta ttgtgcatca
     7321 aaagcaggtg taattggatt tacaaaagct tctgcaagag agttagcatc aagaaacata
     7381 aatataaatg cagtagcacc tggatttata gaaacagata tgactaaagt actaagtgat
     7441 gatgtaaaag aatcaacact agcaaacata ccaaagaaat cttatggtaa accagaagat
     7501 gtagccaatg ccgtagcatt tttagttagt gacatgtcaa gttatataac aggacaagta
     7561 ataaatgtag atggtggaat ggtaatgcaa taataattta aaaagtagag aaatctatta
     7621 aaataaaaaa ttttaggagg tacacaatgt ttaataaagt tgtagaaata ataatagagc
     7681 aattaggagt agaagataaa gaaataacta tggaaacatc attaatgaag gatttagaag
     7741 cagattcatt agatgcagta gaaataataa tggcacttga agatgaattt ggtatagaaa
     7801 taccagatac agaagcagaa aacttcaaat ctataggaga tatagttaag tatatagaag
     7861 ctaataaata gtcgagcttt aaaaaagtgg aggtatccga tgaataaaag agtagtaata
     7921 acaggacttg gatgtgttac gccgttggga acaggaaaag aagagttctg gagtaatata
     7981 aaatctggag tatctggtat agataaaata actaattttg atgctagcac atatcaaact
     8041 cagatagcag gagaagttaa gaattttcat ccagaagaat atataagtaa aaaagaattg
     8101 aagagattag ataaatttgc tcaatttgca atagtatctg ctaaattagc agttgaagat
     8161 gcaaatcttg atttagataa agttgataga gaacgttttg gtgttataat aggttctgga
     8221 ataggtggag ttgaagctat agaaactcaa cataaaattc ttttggaaaa aggaaataaa
     8281 agagtaagtt cactttttgt acctatgatg ataggaaata tggcagctgg tcaagtttca
     8341 atatttttag gagcaaaagg gcctaacact aatgtatgta cagcttgtgc atcaggaact
     8401 cattcaatag gtgatgcttt taaggtaatt caaagaggtg atgcagacat catggtagca
     8461 gggggctctg aagctgctgt aacaggtctt gcttttgctg gattctgtaa catgaaggct
     8521 atgtcaacta gaaatgatga ccctaaaact gcatcaagac catttgataa agacagagat
     8581 ggttttgtaa tgggagaagg agcaggtatt gttatcttag aagacttaga acatgcttta
     8641 gcaagaggcg ctaaaatata tgcagaagta gttggatatg gtttaactgc tgatgcatac
     8701 catatgacaa ctccagctga aaatggagag ggagcagcta ggtctatgaa tatggctttg
     8761 aaagatggaa atgttccatt agaagaagta gattatataa atgctcatgg aacttcaact
     8821 tattacaatg atttatatga aactatggct ataaaaactg tatttggaga aaaggcatat
     8881 gacttatgtg tgtcatcaac aaaatctatg actggtcact tattaggagc atcaggtgct
     8941 atagaggctg ttgtttgtgc tatgagtata gaagatagtt ttgtgcctcc aactataaac
     9001 atacaagaag ttggtgaaga tttggattta gattatgttc caaatcaagg taaagaaaaa
     9061 aatattagat atgcactatc aaattcttta ggatttggtg ggcataatgc tacaatagta
     9121 ctaaaaaaat atgtataaat aaatattaaa tataaaacta aaaaacaagc acttgtttta
     9181 actgtataaa tttatattag cttaaattta aagtctccta aattatttta ggagactttt
     9241 ttattaagat ttaagtctag catatgttgt aaatcatttt gatatattgt attattaatt
     9301 attaagaggt tatttatata tgcaagtttt cattctacaa tatacaaaag gaggactaaa
     9361 attttgaatt ttagactacc aaagaaaagg ttacccaaaa agataatttt tatatgtata
     9421 atacttatta gtattatttt gtttataaag tatgaccaaa atggattgaa agaaagagaa
     9481 attataaata agggaaaata tatgtctgaa agccttgtgg cagataagga gtatgatgaa
     9541 gcaaaaaaag tggttaagac ttctttcgaa tctattccag aaaaaaaata caataaagta
     9601 tataatgaag tttgggatat gttaaagact atttcatatg ttccagatgg aacgaagata
     9661 gtcattgatt ctttagaaaa gatgagagaa tcagatgtga aattatctga tgaatgtaga
     9721 tttaatatag aaaaaagatt agcatcagtg tatataatgg ataataatta ttctaaagca
     9781 gtagatttaa ctgtaaagtc tataaagttg gctgagaaat tggggaataa ttatgaaaaa
     9841 gcaagactag atgttgattt ggcgagcata tttttaaaaa taggtagcta tgaaacagga
     9901 gagaaattaa taaaggattc ttttaaaata gatattgatg aaggtgaaaa aaatggaatg
     9961 gttagattat atgctattat aaatctagca gaaatatatg tagaagaggg agaatatgat
    10021 aaagctattg aaataagtag caggataaaa gattataaaa agtttgtaaa tacggataat
    10081 tacaatgatt ttgatataat ggcctctata atgcagtcaa atgcgtatgt gggcaaaaat
    10141 aatatacaaa aggcaaagac ttttttagaa aaagctgatt atcttataaa atcagataga
    10201 acagtttata tattagataa agatatgtac tattatatgg caatgggaaa cttagaatat
    10261 aaaagcgaaa attataattc tgcaatagat aattataaaa aaagtttaga gatatctact
    10321 aaaagaaagt taacccagga gaaaataaaa aatttaaata agatattaaa tatatatgaa
    10381 aaacaaggaa atatagaatc tttaaatgag tatcaaaaga tgttaataaa tgaatataaa
    10441 gagaacaaaa gtataagaga ttcagagact agtttctata taattgataa agtatcaaac
    10501 gaaagtaagc tatttgaaaa aactaaaaaa gaaataaaat attataaaat attatttaca
    10561 gtaattttaa ttgctatatt agggtctact tttttataca accgattgaa gtattttaaa
    10621 gcacaaaatt tacatgatgg tcttacaaat gtttataata gaaaaagttt tgatattatg
    10681 tatgaaaagt acagagagaa agatgataat tttgcacttg tgatgataga tattgataat
    10741 ttcaagctca taaatgataa ttatggacat aaatttggtg acactgttat aaagggaata
    10801 acaaatacca tttgtgttat gttagaacaa gatgataaag tctttaggta cggaggagaa
    10861 gaattttctg tattaataag aaataaatct ggtgaggaag tagcagatat aattgacagt
    10921 ataagggttg caattgcaaa taaaaaatgg aatgatgatg tgactgtgac tataagtgca
    10981 ggtgtagctc atatttcaga tagcaaagat gtactagaag aagctgataa aaatttatat
    11041 aaagcaaaac aacttggtag aaataaagta gtatataagt aattatatac tatataaata
    11101 agaggtataa atatgaaaca atcaatagct atagtaactg ataaaataac taaactatca
    11161 tctttcttag aagaaaatat acgtttggtt ttaggtaatt atattgatat aaaccactat
    11221 tatctggaag ttttaaaaga gaatgataaa atacaaggtg attttgtact agtaatgaat
    11281 gatgatagat taaacagtat gagaaagcat ttagtacaag atagtaagat tatagtgata
    11341 aggagaacac ttaaagaaaa tgaaatatat ggattatttt caataccaaa tggaactgaa
    11401 gtattggttg taaatgatac aaaagaaact accttagaaa cgataagctt attttacaaa
    11461 ataggagtaa aaaatttaaa gtttactcca tatattgaag gaaaaattta taaaaatata
    11521 gacatagcta taacgcctgg agtaaaggga aaagtaccaa gttatataaa taaagtaatc
    11581 gaccttggaa atagatatat tgatatatct acctttatag aaataataaa tggtcttaga
    11641 atagatgtta aagaaatacg taaaaattta atggcatatt ctgagagttt aataagttta
    11701 gataatggag ttaaagataa ttataggcaa ctgtttttaa aaatagaaga acaagatgtt
    11761 atattgaatc tatcaaaaga tggaataata tttacttcag cagatggtat aataaacaca
    11821 tttaataaag aagctttaaa aattttaggt ataaatgaaa atccacaagg caaaaacata
    11881 gaagatatca tttctgaaga tttaaatatt ttacttgaag acaaagaaat aattgatgaa
    11941 gtggtattta caaataaaaa atatataaat gtaaataaaa aaagtatttt ttctatgggg
    12001 aataaagctg ggatatatta taacttacaa gaaataacgt atataaaaaa attagaacaa
    12061 aatcttacta ataaattaag agaacaaggt cagatagcaa gatatacatt taaagatata
    12121 aaaactaaaa ataaaaatat ggtaaagtgt gtagaattgg ctaaaaaaat atctaaatca
    12181 gacctttctg tattaataat aggagaaagt ggtactggta aagaattatt atcacaatca
    12241 attcataatg cttccaatag aagtaatcaa ccgtttatag cagttaattg tgcagcagtt
    12301 ccagatagtc tattagaaag tcaactgttt ggatatgaaa aaggctcttt tacaggagct
    12361 ttaagagaag gaaaaaaggg tctatttgaa cttgctaata atggaactat atttctggat
    12421 gaaattggag atatgcctcc attgctacag acaaagttac taagggtatt acaagaaaag
    12481 caggttatgc ctataggttc ccataatgtt ataaacatag atgttagggt catagcagca
    12541 acaaacaaaa acttactaaa gatgattaga gaaggaaaat ttagagagga cctgtattat
    12601 agactaaatg tattgccaat agatgtacca tcattgagga atagaaaaga agatataata
    12661 acacttatga atttcttttt gaaaaacaat ataagacttt cgccagaagt tataactatt
    12721 ttagaaaaat ataattggcc aggaaatata agagagttac aaaatgttgc ttcttatgta
    12781 agtctaatgt gtgatagtat ggtttttaga gatgacttac caccatatat aagggtagaa
    12841 tgtgctaaca attatgaaga tggatttggt tatacaagaa aagaatttga ggaaatactg
    12901 aggattctat atgaaagaag agagtcatcc tctggaattg gaagagggtt tataatcgaa
    12961 aagcttatac aggaaggtat tgagatttct gaaagcaaag ttagaaaggt gctatcttat
    13021 ttaagtgaat gtggatacat atcatctaaa tctggtagag gaggaagtca aataacctca
    13081 gaggggataa aactttataa taatataaat taagctaagt atttgatatt gaaatcacat
    13141 attttgaatt tagatattta aataataata aacttttata tttatagaga ttaatttata
    13201 ttatgtcaga aagaatattg taatttataa ttaaatatta ataggttttt aaaataggat
    13261 tagataggta ataggttaag ttagaataac ccattaccta tttttatttg taagaaaagt
    13321 gtacatagaa ttagaagtaa aaaatacaac taataaaact attgaaaata ctagataaat
    13381 aataaataaa aataattata gattttggca tacatattgc taattataga gataagacga
    13441 caaaataatt gggaggttta atgatgaaat acaaaacaac agggacttgt gctacagaaa
    13501 ttgaatttga agtaaaagaa aataaagtta caaatgtaaa ctttataggt ggatgtgatg
    13561 gaaatttaaa agggctaaaa gtattagttg aaggcatgaa tatagaagat gtaatacaaa
    13621 agttaaaagg aatagagtgt aaaacaaagc caacatcttg cccagaccaa ctgtctctag
    13681 cattggaaaa ttatatgaat ataaagtaat acataaaaca atcgattagt taggagttta
    13741 attatgaaac caataatagg gattttagga aatttaataa taatggaaaa tggtatgttt
    13801 ccaggtcttg aaaggtcata tgtaaataat gattatataa atgcagtgct aaaaggtggc
    13861 ggtagtccag taataattcc tgtaaataca gataaagaag ttataaaaaa acagattgaa
    13921 atggttgatg gagtcttaat atctggggga tgggatatca atcctcaact gtatggagaa
    13981 gaaacaagag aagaaacgac atttatatat ccagaagtgg atgaatttga tttaattgca
    14041 ataagtatag cattagaact taaaaagcct atattaggtg tatgtagggg actacaaatt
    14101 ttaaatgtaa gtttaggagg aactttatat caagataata atcttataga aggaagttat
    14161 ataaaacata cacaatcttc taaaagacat gtagctactc ataaagttga tgttaaagaa
    14221 ggaagtattt tagagggaat tttaggaaaa caattactta caaatagtta tcatcatcaa
    14281 agtgtcaatc agcttggaaa aggattaaaa gccatagcat actcaaaaga tggtataata
    14341 gaagctattg agaaagagga tgaaaatttt gtagtaggag tacagtggca tccagaaatg
    14401 atggtagatt actgtgataa aatggaaaaa ctatttaaat attttataaa tatttgttcg
    14461 aatttataat ataccttggg gggattgaac atgaacgaaa agaacaaaat gggattaata
    14521 agtattatac ttttaggtat taatgcagta gtaggagctg gagtttttct attaccagga
    14581 gatgcaatga aatcatttgg tgtagcaagt atatttgtat atatatttga tatgttgtta
    14641 gttttatcta tggcattctg ttttgctgag gttgcaggta aattcaataa aaatggtgct
    14701 gcctatgtat atacaaaaga agcttttgga gatttttgtg gttttgaagt aggtcttatg
    14761 aaatgggtaa taggatgtat ttcttgggga gctttaatag taggttttcc tacatcatta
    14821 tcagcagtat gggctccagc aggtgaacca catatacaaa aaattataat tgtagctatg
    14881 atagttgggc ttacaattat aaacttatta ggtgtatatc tatcaaaaat tgtacaaaat
    14941 gttataactg taggtaaact aattccatta atactattta taggaatagg tatatttttt
    15001 ataaaaggag ttaattttac ttcttcaaca atggtacctc caggagctgg agcaacagag
    15061 tttggagcag cagcactttt aatgttttat tcatttacag gatttgaatc tatagcagtt
    15121 gcagcagaag atatggaaaa tccacaaaaa aatataccaa tagcaattat aagtgtaata
    15181 gtaattgcat caataatata tatattaaat caagttgtat gtgttggtat actaggagat
    15241 tcgttatcta gtacatctac accagtggca gatgctgcaa gaatatgctt tggaaatatg
    15301 ggagctggat tggttacctt tggaacattg gtttctgttg gtggaatttg tatgtgtggt
    15361 gcttttgtaa atccaagaag ttgtgtagct ttagctgatg ataaaatgtt accgagaata
    15421 tttgcaagaa aagataaaaa aggtacacca tatgtagcga taatagctac tatgttaatc
    15481 actataccaa tagctctatc tggaagtttt gctgaattag cagcaataag tgcagttgct
    15541 agattcatac aatacatacc aacatcttta tccgtattag tgtttagaaa gaaaagacca
    15601 gaattagtag gtacttttaa aactcctttt ggagcagtga ttcctctaat tgctgtatgt
    15661 gttggaacat ggttgttatt ccaagcatct atgcatcaat taattatggg attaggagct
    15721 ttggcaattg gagtaccatt atactttata atgaagtctt ataatagaaa aatctatggt
    15781 gaaaatttaa agaaaatagt ttaattatgt tataacaatt tattgagtta aaattagaca
    15841 ttggcagaaa attaaataca aataagtatc tacaaggcta tattaaaata ttattaattt
    15901 tttattttaa tatagcctta tattgttatc aataaatgaa aaatattgta acaacagaag
    15961 aacaatagta ttatgtttat agacaaaaaa cttattagga ggtaaattta tgccaacaat
    16021 aacattaaaa aatggtgtag acgtacttat tagagaaggt gtaagagaag atgctcaaag
    16081 cattatagat ttttataatg aagtgggtgg agaaactcat tttctgtcat ttggaaagga
    16141 tgaatataaa atatctttgg aggaacaaga aaatgctata gaatcagcaa aagcatctga
    16201 taattctgtt aaactgattg catttataga tggtgaaata gttgggattg caacgattga
    16261 ttctaatcaa aaagcaaaag gaaagcatgt tggagtatta ggaattgttg taaaagaaaa
    16321 atattgggga attggattgg gtaaaaggct tatgttagac cttatagaat ggtgtaaatc
    16381 aaatggaata accaagaaga taactcttgt cactaatgaa gaaaattata atgctatagg
    16441 attatacaaa aaagttggat ttgaagtaga aagcatacta aagaaagaat gttattataa
    16501 tggtgtttat actgatttaa taggaatgtc acttttattg ggaatataat tagtgtacat
    16561 tttatctcga aaaaagtata ttaagtatgg tataattaaa aaagaatata acttaatata
    16621 attttggagg acgaattatg aaaaatctgt gtaaaatatc agatgattat ttaaacagca
    16681 agcttaagta tttaaagtta ttatcaaaac aatatccaag tatatcaaaa gcaagtacag
    16741 aaataataaa tttagaggct atattaaatc ttccaaaggg aacagagcat tttataacag
    16801 atgtacatgg tgaatatgaa ccatttgtac atgtattaaa aaatggttct ggtgtaataa
    16861 agagaaaaat agaagaattg ttttcaaata caataagaga cagcgaaaaa aaaatgttag
    16921 caactcttgt atattatcct gaacaaaagt tagacctaat aattaagcaa gaggaaaata
    16981 tagatgattt ttatagaata aatatttata gacttataga actttgtaaa tatgcttcta
    17041 gcaaatatac taggtctaaa gttagaaaac tgttaccaga aaattttaaa tacataatag
    17101 aagaactttt gcatgagcat gttaaaagtg aacataaaga agagtattat aaaagtatag
    17161 ttgaaacaat agttgacata ggaatagcta aggagtttat aattgctatt tctacagtta
    17221 tacaaaaatt ggttgtagat agacttcatg taataggaga tatttatgat agaggtccaa
    17281 gaccagatat aattgtcgat aagcttattg aacatcattg tgtagatatt caatggggaa
    17341 atcatgatat attgtggatg ggtgcagcct caggtgaaaa aacttgtata gcaaatgcac
    17401 tgaggatatc agctagatat gcgaatttag acattgtaga agacatatat ggaataaatc
    17461 tattgccttt agccactttt gctatagaaa tgtataaaga tgacccttgt aaagaattta
    17521 ttccaaaggt taatgaccaa agtgtaacaa caacagaaaa gtctttaatg gcaaaaatgc
    17581 acaaagcaat aagtataatt caatttaagc ttgaaggtga ggttatcaga cgaagacctg
    17641 aatttgaaat ggaacataga ctcttgctta atatgataaa ctatgatgaa ggtacgatta
    17701 atttaaaagg taaaacatat aaattaaaag atacttattt acctacaata gacaaaaaag
    17761 acccatataa attaactatg gaagaaagaa atgttattga taaattagta tcttcattta
    17821 gaggtagtga aaaactacaa aaacatgttt catttttgtt ttctaaagga agtatatatt
    17881 taaaggctaa ctcaaattta ctgattcatg gatgtgtacc gttaaatgaa gatggaagct
    17941 ttatgtctat gaatataatg ggaaaagagt ataaaggtaa ggctcttatg gataagatgg
    18001 agtctctagc aagagaagga tttttcttta aagataaggc agaagaaaaa ctgtatggca
    18061 tggatattat gtggtattta tggactggga aatgttcatc attatttggt aaagatgata
    18121 tgactacatt tgaaagatac tttatagctg aaaaagagac tcataaagaa aataagaacc
    18181 catattttaa acttagagaa aatgagatgg cttgtaaaag attatttgaa gaatttgatt
    18241 tagaacttga tgaatctcat ataataaatg gtcatgttcc tgtggagagt aaaaatggag
    18301 agagcccaat aaaagctaat ggtaaaattc tcgttataga tggtggtttt tctagggctt
    18361 accaaaaaac tactggtata gctggataca ctttgatata caattctaga actttgcaat
    18421 tagtatcgca tgaaccattt aattctgctg aagaagccat tgcaaatgaa agtgatatct
    18481 tatcgacaac tgttgtagta gagcataagg caaagagaaa aatggtgaga gatactgatg
    18541 aaggaataaa gatacaagag gaaatagaag atttaaaact acttttaatg gcttataaga
    18601 aagggctaat aaaagaaatg tagatgttat agaggcactt catgattgaa gtgtcttttt
    18661 gttaatactt attaaatata ttataaagat taataatagt agtttatatt atacttacta
    18721 agtgaatcca agataaatgg gttcactttt tttatttata aacattatta aaaaaatata
    18781 gatgttttgg ttatattcct tgtcctttat aaatagaatt atataaacaa aggaggagga
    18841 caagagatga ataaactttc tgatgaaata ataaactctc tttctacaac tataagagaa
    18901 aaggtagaaa aagtctcaaa caataatctg aacatagaag aaattaggtt acgctcacaa
    18961 aaaccattga tactaaatgc aaactctaaa gattattttt acaatcaaaa aactatgaca
    19021 cttgatttaa atcaacaaaa ctcatatgta gtaacaagag aagatgtaga gcaaactttt
    19081 caaataatat gtaaatattc aatacattca tttatggatg acataaaaaa aggattcata
    19141 actttgagag gaggtcatag agtagggtta gttggaaaag caatagtaga agatggacaa
    19201 gtcaaaaata taaagcatat atcatcttta aatataaggg tatcaaggga aattattgga
    19261 tgctcggata agattttaag tcatatcata aagggaaaaa atcagattaa taatactttg
    19321 ataatatcac cacctcaatg tggaaaaaca actttaataa gagatatagt aagaaattta
    19381 agtaatggta atgaagatta tggatttaaa ggtttaaaag tggctttagt agatgagcgt
    19441 aatgagatag caggagctta tctaggtgtt ccacaaatgg atgtgggaat aaggactgat
    19501 ataatagaaa cttgcccaaa agacttgggt attacaatgc ttttaaggtc tatgtcacca
    19561 aatgttattg tgacagatga aattggaagt gaaaaagaaa taaaggcatt atacacagca
    19621 ttaaatggag gtataggatt aataactact gtgcatggag attcaataga ggatatacaa
    19681 aatagaaaag aattaaatag acttctggat aaagagttat ttaaaaaggt tataattctt
    19741 tctgcaaaaa gaggagcagg aacaattgaa aaaatttatg atttagaaga aaagagatgg
    19801 tattttgcaa attaaaataa ttattatagc tttcttgata ggaagtagtt atttgatagg
    19861 tgaacaaata tacaaaactt atactcgtag acataaacaa ttaaacgatt taataagagt
    19921 tttagaaatt ttgaggatgg atttatcttt tggactttat acattagaag aaatctttaa
    19981 taggataggt ggaaataaag agttttgttt ttggaaattt ttctatcaaa tatcagaagg
    20041 acttcacaat gaacaaagta aaactttgga aataataata tcagaaaata ttgatgtctt
    20101 aagtaaagaa acttatctag gcaataaaga aattgaggaa ctgaaaaatc taatacttac
    20161 tttagggaaa agcgatatag agtcacaaca aagaatgata gatttatcta tagaaaattt
    20221 gaaaaaacaa acctatgaga caaaggaaga tataaacaaa aaaggcgtat tgtataaaaa
    20281 attagtgaca ttcataggta taggtatatg tattatattg atttaatatg gggaggttta
    20341 ttatggaaat atcattgata ttaaaggttg caggtgtcgg tattctaata tcagtattga
    20401 atatgatatt ggaaaaaaca gatagaaaag attgggcagg tcttactaca ttagcaggtg
    20461 taataatcgt attgggtatg gtaataacag aaataagtga cttgtttaat actgtaagaa
    20521 caatgtttca actttactaa agaggaggga gtaatttgga aataatgcaa ttaataggaa
    20581 ttgcaatcat ttcaactaca ctctgtttag taattaaaaa agatagacca gagatagcaa
    20641 atttcatagc tataataact ggagtaataa tactattgtc agttatgttt aagctaaatt
    20701 ttatagtaga tagtatacaa gatttggcta acaaggctaa catcccgact atgtatatat
    20761 cattaataat aaaattaatt gggatagcat acttaatgga atttgcgata caactttgta
    20821 aagattgtgg ggaaggaaac attgcttcaa aactagaatt tggaggtaaa ataatagtta
    20881 tgtcaatgtc tttccctata cttttgtcca ttgtagaaat ggtggtgaat ataattccat
    20941 agatgtgtat cttggaatga atataagtat attagagagc tttcgttgta ttgaggttta
    21001 tagagtgaag agaggcatgg ttgcttatga aaaaaagatt tttaccaatg attataggtt
    21061 ttttatttac tttctttttt gtaaatatct ttgctattat gatatttgct aatgaagtac
    21121 caagtagtga agataaagaa tatggtgaaa caaaaaatag catagataag tatatagatg
    21181 gtcaattaga caagctagat ataaatgaga ttcaagacta tataaataaa gaaatagtta
    21241 ttaatgatgt taatttaaaa tcatttgtaa aagatttaat tagtggagaa aaaaatatct
    21301 tagatttatt caataaagat ggattgaaga tattgatgtt tgatgaattt aaagcaagtt
    21361 taaaggtagt agcagtaatt ttggtactgg ctctgttatc atctatttta aagagtctgg
    21421 aaaactcttt ttcatcaggt gcagtaagtc aaatagcaac atatataata tttatcacaa
    21481 tggtatcact tacacttatt ggttttaaag atgtattaca gatttgctat gatgcaatag
    21541 accatactgt aggtcttatg caagttataa tgccaatatt aataacattt ttacttttaa
    21601 ttggattccc aataacgtcg actactttga atcctatctt cataggagga gtaacattta
    21661 taaatgtatt ttttaaaaat tttctatttg tatctattac tgttgcattt ggaattttaa
    21721 taataaacaa tttatcaaaa aatataaggc taaagagatt tttctccttt gtaaaacaaa
    21781 taaattatgt ttctattgga gctatgttta ctgtttactt aggtcttgta tccatacaag
    21841 gactgtatgt aaccagtttt gataaattca gtgtaaagac tgctaaattt gctataggta
    21901 attttattcc tgttgtaggt ggatttgtat ctgactctgt ggatatttta ttatcttcat
    21961 ctcagcttat aaaaaatata tttggaggaa tagggcttat cttacttgtt ggaatttgct
    22021 tacttccagt aataaaaatt ttatcagtaa tagtagtgta caaattagca gcaataattg
    22081 tagagcctgt tggagaggat ggaatatcta attttttaaa tgaagtggca aatttaatga
    22141 taataatgct agcatcagta attgctataa cagtaatgtt ctttgtaact gtagctatct
    22201 tgacctcgat aagtgtcgta agtcaagggt aatttatata tcagtgaaaa ataattacat
    22261 ttaatctaat gtttttagag aggaggatgc tatgttagag ggcataaagg catggatagt
    22321 tagtgttttg ataggagcat ttatagtaaa tatagtagat atgattttac caagttctaa
    22381 gataaaacct tatgtcaacc tagttttaaa ctttatgttt gtatttattg taataactcc
    22441 agtagtagga tttttttcaa aagatatgag tttagaagat agaatattaa aatctatggg
    22501 aaactacaac aagcagtatg tagatagtac aaatgctttg gcaaaagaga ctggaaataa
    22561 tagtctatcc aagggatatg aggatgggtt aaaagaagta cttaaattaa aacttgatga
    22621 atatggttat gatttagaag atatagagct taatggggca aatataaata atataaaaat
    22681 aaaagaaaag aataatagtg caaaaagtaa taacagttcc aatgaagaaa ataaaaataa
    22741 tagtactaaa agagaggaag aaaacataaa ctctaatgac aaagaaaatt ctaaacaagt
    22801 cttcaaaaaa ggtacagagt atgggttgaa cctaaatgaa gaaaaattaa aaaacgactt
    22861 aataaaggtg ctggatgttt ctatagaaga tatacaaatt gataaatagg agttgggaga
    22921 atgttgaaaa acttaaatga caaagataag agaaaggtat attcactaat aactatagca
    22981 ggaatatgtg tggtttcatt agtattatta tcttgttttc ctagtagcaa aagcgataaa
    23041 caagttggaa aaacagagac taataaaaat gctgaaaagc aagtcactaa agaacaagaa
    23101 aaagatgatt tagagtcaaa attaacagca atactatcta aaattgatgg tgcaggagac
    23161 gttgatgtaa tggttacatt tgaatcaagt gaagaaatac aacctgcttt taattcaaat
    23221 aatacaacag aaacaacaga ggaaaaagat gcacaaggtg gagaaagaac agttacgact
    23281 tctagtgaaa ataaaacaat gataacttca aattcaagtg accctgtagt tataaaaact
    23341 acagagccaa agataaaggg agtgatagtg gtagcaagtg gagcaagtga cccaagtgtt
    23401 aaagagacac tttatagtgc agtacaaaca tcattacaag tagctggtca tcaagtagaa
    23461 atatattcta aataaataca tataaccggg gggataaatg aatgaagttt aattataagg
    23521 gaagaggatt tgtaataata actttaactg caatgttagt ggtagtagga acagtgaatt
    23581 atcaactaag taagaaatct ttattagaaa catcaaaaga gtttaaggca tatgaagaag
    23641 cacagttgca aaaaaatact gatgagagtg atagtagtaa gacagaggat tctagtaata
    23701 tagataaaca agatggtaag gaaagtgctg atattgatat agttgatagc aaagctagta
    23761 aagtaaaaga aaaggcaact gagacaagta aggaaataaa agctcaatta tcatcagaga
    23821 aaaatatgaa aaaggcatct tatattttag atatgaaaat gaatagagaa aaacaaagaa
    23881 atgagttagt tcaagattta aatgaaatga taaataatcc atctacaaca gaagaatcta
    23941 gaaaagaagc ttctaatatg aaattaaata tagttaaaat tcaagaaaaa gaacttcaaa
    24001 tagaaaacct tttgagtaca aaagggtatg aagaagcgtt agtttatata agcgataaca
    24061 aagttaatgt agttgtaaat gaagctaaat tagaaaagaa agatgcagca aaaatatttg
    24121 atttagttgc agaacaagcc aatgtgaagt atgaaaatat taaacttaca aataataata
    24181 gtaataagta aagtataaac ctcagaatta actgaggtta ttttcttgca aagtagactt
    24241 gtatttggta ttattatact tataaaaata gttccttaaa atcaggaggt aagttttaat
    24301 atggaagata ataaatttgg acaagtaaaa atatctaatg atgtaatagc tacaatagct
    24361 ggtttagcag cattagaagt tgaaggcata gaaactacag caacattaac agataagttg
    24421 ttaaaaaata atggagtaaa aatacaaatc gaagaagagg atgttaattt agatgtaatg
    24481 gttacgataa agtatggaat gtcaatacca gatacagctt ttaaagttca agaaaatgta
    24541 aaaaatactg ttgagacaat gacaggactg aaggtttctc aagtaaatat acacattcaa
    24601 ggaatcagct ttaagaagga taaagttgat aaagaagaag caaaagcaac taagaaaaat
    24661 taatattagc cctctgaaaa gtcagagggt tttttgctgg taattgattt tatatggata
    24721 attaaactta aaggagaatt ttgattgatg aaaaaagata gggcacaaaa gagtacaaca
    24781 agagaatata taatgaaact tatataccaa ataaatatta ataaagaaga ttttgaaact
    24841 ttagaagata aagtagataa ttttttaaaa gataattcag aacatattat aaatagatat
    24901 aaagaacttg cacttcaata ctcaaaaaat acaaatttaa agttagaaga tactgaaatt
    24961 gaagatgtta tagacaaaaa atatataaat acagtatgca aagcattgaa agaaaatcat
    25021 gacaaaatag atgagttaat aaataagcat gctaaaaact ggacagtaga tagaatgcca
    25081 aaggtagatg tttctatact tagattgtct gtgtgtgaaa tactttattt agacactcct
    25141 aataaagtat caataaatga agcagtagaa cttgcaaaaa tatattgtga tgataagtct
    25201 cctaaattta taaatggtat attgggaagt gttgttgatg aaattgggaa ataatataat
    25261 aattggaatt gatactagct gctatactac ttctatagca gctatctctt tagataagaa
    25321 agttatattc aatgaaaaaa taatgttaga agtaagggat aattcaaaag gtttaaggca
    25381 aagtgaagct gtatttcaac atatcaataa tttagggatt cttagtgata ggataaaatc
    25441 ttttaaagat aagtttaatg tagaaggagt ttgctcatca aaaaaaccga gacctgttga
    25501 aaactcatat atgcctgtat tcaatgtagg tcataatttt ggaaaattat tgtctagtat
    25561 atatggttgt aggttttatg agactactca tcaagagaat catattgaag caagtttatt
    25621 aaatagtaaa ttaaaaaata ataataaatt tatttctgtt catatgtctg gtggtactac
    25681 agagatactt ttaacaagca aacaagatag tcatcacaat gtatgtgata ctaatttagg
    25741 taaaattgcc aaaataagta ttaaaaaaga tgataaaagt aagttataca ataattttgg
    25801 atataatata gatataattg gaggaagtaa agatattagc tttggtcaac ttatagatag
    25861 agtaggaatt aagcttggat ataaatttcc ttcaggaaag tatttggatg aaaatgcttt
    25921 aaattgtaat cttaaaatag aaagtggtct gaaaacttct gtaagagatg ggtatatgaa
    25981 tttatcagga ttagaaaatc aggtaaataa gattataaat gataatggat ataatactaa
    26041 tcaaaaagaa tatatatcta aattggtatt agattcagta gttagaaata tgtttaaatc
    26101 tttagtgtat ttatgtgaaa cttataatgt aaatgaagta gtatttgctg gtggagtttc
    26161 tgcaagtaag tatattctta gagaattaag cgtgaagtta agaaaaaagc atatagaagc
    26221 ttattttaca gaaccacaat attcaactga taatgctgtc gggtgtgcta taataggatt
    26281 gaataacttt ttaggagaaa gagtatgaaa ctaagagctt tagatataag tgaagcaaac
    26341 tcatatatta aaagaatttt aataaacgac ccaatacttt ctaatcttaa agttaaagga
    26401 gaaatttcta attttaaggt tcatagtagt gggaatgtat atttatcact taaagatgaa
    26461 acatcaaagt taaactgtgt aatttttaaa agtaatttta atcgcaattt aaaattagat
    26521 aatggtgtaa aaattatagc taatggatat atatcagtct atgaaagaga tggtgcgtat
    26581 caattataca ttaatgaaat tgaaatagag ggaataggaa atttgcacat agagtttaat
    26641 agattaaagg aaaaacttaa taaagaagga ttatttgacc ctaaatacaa gatacctata
    26701 cctaaaatgc caaactcaat tggcgttata acatctccaa caggtgctgt tataagagat
    26761 ataataaatg taataaaaag aagatatccg aaagttaata taaagcttta tccagtaacg
    26821 gttcaaggag ataaatcagc tgaagaaatc tgtgaagcaa ttagattttt caatcatatg
    26881 aagaatgttg atacacttat agttggtaga ggtgggggtt caatagaaga actttggtct
    26941 tttaatgatg aaatggtagc aagagaggtt tttaattctc aaattccgat aatatcagca
    27001 gttgggcatg aaacggattt tactatttgt gactttgtgt ctgatatgag agccccaacg
    27061 ccatcagcag cagctgagat tgctactcca tcattggatg atataaacta taaacttgga
    27121 aacataaaaa gtaggatgag taagtctctt acaaatcaaa tagagctaga ccaatatagg
    27181 ttagaaacag tatttaataa gataaataac tatttagatt cgtacacaat aaaagataaa
    27241 gttatacaat tagacaaaat atatgataaa ataatttttg gaatagaaaa taatttaaaa
    27301 ttagaagatg aaaaattagt aaaaattgga gcattactcc ataatttaag tccattagct
    27361 actatggata gaggttatag tataactcaa aaaaatggaa aagtaataaa tagtataaaa
    27421 ggattaaaaa taaaggatag tatagatata gtattaaaag atggaaattt agaatgtatg
    27481 atagataaaa ttgaaaataa agagggatag tatgaattta acctacgaag aagcttataa
    27541 aagattagaa agtattttaa gtgaattaga atctaaaaat gctagcttgg atgaatcttt
    27601 aagcctatat gaagaaggta taagccttta taaacattgt aataagcttt tagatgatgc
    27661 taaattgaaa ataagtaaat ttaatcaatt gggaatagaa gaagacttta aaatagagga
    27721 ggaataaaat tggaatttaa acaatgtcta aaagaaaaag caagttttgt agaaaaagta
    27781 cttaaagaat atatgcctaa agaagaaggg tatcaaaaaa ctgttattga agctatgaac
    27841 tatagcttaa gtgctggagg taaaagatta agaccaatac ttacattgga ggcttgtaaa
    27901 atagttggag gtaatgaaga tgaagctatt ccttttgcta ttgcaattga aatgatacat
    27961 acatattcac taatacatga tgatttgcca gctcttgata atgatgattt aagaagagga
    28021 agaccaacaa atcataaagt atatggagaa gctatgggga ttttagcagg agatgctttg
    28081 ttaaactatg cttttgaagt aatgcttgca ggctcaatca ataaagaaaa tccagaaaag
    28141 tatctaaaag ctataaatga aattgccaaa ggtgctggta tatatggaat gataggtggt
    28201 caagtagtag atgtagaaag cgaaaataaa caaattgaaa aagaaaaatt agattatata
    28261 catatgaata aaactgctgc aatgatggta ggctgtatga gagcaggagc tactataggt
    28321 ggagcaaatt ctgaacaaat ggaagaaata actaaatatg caaaaaatat cggattatca
    28381 ttccagatag tagatgatat actagacata gtaggagatg aagctaagtt aggtaaaaag
    28441 gttggaagtg atatagaaaa tcataagtca acatacccat ctcttttagg tttagataaa
    28501 tctaaagaaa ttgcacataa tttgattgat gaagcaaaga aaagtataga aaaattatct
    28561 gatgatgtag attttttaaa gggattggct gaatatataa tagatagaga atactaaaat
    28621 atacagctaa aatcgaggag ggaaaaatgg attttttttc ggagattttt aacaatgggg
    28681 ctttgggaat aagtctgatt gcttgtttct tagcacaatt tataaaaata tttactggta
    28741 aggaaaaaag aattgagtta tcaagaatac ttatttcagg aggtatgcct agttcccata
    28801 gttcttttgt tacaagttta gctacagtgg taggtattga aaaagggttt aattctactg
    28861 attttgctat aattaccgtg cttgccttaa ttataatgta tgatgcagca ggtgttagac
    28921 gggctgttgg taaacaagct actatattaa atcaaatggt ggcagatata cagcatggaa
    28981 agcatataga gcagaagaaa ttaaaagaat taataggtca tacaccacta gaagtttggt
    29041 ttggagcttt acttggaata gtaacagctt taatattgat gtaaattgaa aatttacaga
    29101 ataaaggtga ggataatatg tataaatatt tagataaagt aaattctcca aaggatataa
    29161 aaaatatgag tatagaagaa atggatttac ttgcaaagga tataagaaag ttcttagtaa
    29221 aatctgtttc aaagacagga ggacacttag cttctaatct aggagtggtt gagttaacat
    29281 tagcacttca taaagtattt gatagcccta aagataagat tgtatgggat gtagggcatc
    29341 aatcttatgt tcataaaata gttactggaa gaaaagattg ttttgtatct ttgagacagt
    29401 ttaatggact aagtggtttt ccaaaggaga atgaaagtcc tcatgatatt tttgatacag
    29461 gacatagcag tacatctatt tctatagcta caggtattgc ctgtgctagg gatataaaaa
    29521 aagaaaacta tagtgttata tctgtgatag gagatggttc tataacagga ggtatggcac
    29581 ttgaagcttt aaatcaatta ggatatatag ataccaatat gatagtaatc cttaatgata
    29641 atgagatgtc catagataaa aatgttggag gaatgtctaa atatctgtca agtattataa
    29701 gaaactcaac agttgagaaa atgacagatg aagttgataa aattttaaat gttacacaaa
    29761 caggtgaaat tttatctaag actgcacata gatttaaaga taaactaatg tatagctttt
    29821 ctcctcagga ctgttcattt tttgattcat taggtataag atattatgga cctatagatg
    29881 gccataatac taaagaacta atagatatat tgagaaaagc taaacacaaa aaagggcctg
    29941 ttcttttaca tgtaattacg aaaaaaggaa aagggtacag atttgcagaa gaacaacctg
    30001 ataaatatca tggagtatca aagtttgata taaagacagg tgttacatct gcaaaagtta
    30061 agtctatgtc aattagtgtt ggagaaaaac ttgttgatat ggctaatagt aatgaaaata
    30121 tagtagcaat aacagctgca atgccatctg gtacagggtt aaacctgttt gaaagtgcat
    30181 atccaaaaag atattatgat gttggtatag ctgaacaaca tgcaacagga tttgcagcag
    30241 gtcttgcaaa aaatggtatg aaaccttact ttgctgtgta ctcatcattt ttgcaaaggg
    30301 catatgacca agttattcat gatgtatgca taacaaaaaa gccagttact tttcttatag
    30361 atagagctgg attagttgga aatgatggag aaactcatca tggtatgttt gatttaagtt
    30421 acttaaattc tattccaaat attgtagtaa tggctccaaa agacacaaga gagatggagc
    30481 ttatgatgga tttgtcatta aaattagatt gtccactagc aattagatac ccaagaggaa
    30541 gtagttatta cctagataag ggagagtatg gagagattgt actgggtaaa tatgaggtat
    30601 tagatgacgg tcaagataca gtcatacttt gtattggaag tatggtaaaa catgctttag
    30661 aggctaaaga aatattatca agagaaggaa taaatccgac aatagttaat gctagattct
    30721 taaagccaat tgatgaaggt atgttaaaag cattattaaa aaatcataaa aatgtagtta
    30781 ctatagagga taatattgta actggaggat ttggaagtag aataaataag tttattatag
    30841 ataatgaata taacgtaaat atacttaata tagcaatacc agaagaattt gtaaaacatg
    30901 gcaatataga tgaattatat gattttgtag gattatcgcc aaaaagtatt gctgataaaa
    30961 taaggaaatt agttattgag taaaaatcta atgtagatat tgtaaattaa aatttatata
    31021 aaatatagaa tttatcaatt agaataaata tcaagtgtgt tacttcaagc tactttagtt
    31081 atcattaaag tagcttttaa cagaaaggta attgtatgaa aaaaagaata gatttattat
    31141 tagtagaaca agggtacttt gaaagtagag aaagagccaa aaaagcaata atggctggat
    31201 tggtttttgt tgataatcaa aggtgtgata aggctggtac agaagtaaaa gaagattgta
    31261 gtatagaagt aaaaggaaat ccaataccat atgtcagtag aggtggttta aagcttgaaa
    31321 aagcaatgaa aaactttgat ttgactattg atggaaaggt atgtatggat ataggggcat
    31381 ccacaggtgg ttttacagat tgtatgctca aaaatggagc aataaaagta ttttctatag
    31441 atgttggtta tggtcaactt gcatggaaat tgagacaaga tgatagggtt gtgtgtatgg
    31501 aaagaaccaa tataagaaat gtgacaatag aagatacaaa gcagtttgct gattttgcgt
    31561 caattgatgt gtcttttata tcgttaaagt tagtacttcc aaaggcaaag gagttagtac
    31621 gtcatgatgg ggaagttgtc gctttaatta aacctcaatt tgaagcagga agagaaaaag
    31681 taggtaaaaa aggagtagtt agagaaaaat ctacacatat agaagttata aaaatgattt
    31741 ctgatttctc agtcgaaaat ggatttgaga ttttagggtt agatttttct cctattaaag
    31801 gtcctgaagg taatatagaa tatctaattc atttgagaaa tggaaatgaa ggatatgaat
    31861 ttgatggtga gacatacaat aacaaaatag tagaagttgt tgaagcttct cataatttag
    31921 acaaataggt taaaaggggg cttagatttg atcctcgaat tatatatgaa aaattgtgcc
    31981 ttagttgaag aactaaggtt gaatattgat aaaaacttaa atatacttac aggtgaaact
    32041 ggttcaggaa aatctataat aatagatgca cttggacttt gtttaggtga taaatatgat
    32101 aggtcttttt taagaaaagg aacagataag ggacttgttg aggctgtatt tttttcagac
    32161 aatagatatc ttaaaaaaat attagaagaa aatgatataa gtatggaaga tgataattta
    32221 ttggtaataa ctaggttgat atactcagat ggtaagagta ctgctagagt aaatggaagg
    32281 actgttaaag tgtctcttct taaagaaatt gcatctacac ttatagatat acatggtcaa
    32341 catcaaaatc aagctttgtt taataaagat actcatttaa aatttctaga cctttttgga
    32401 gaaaatgaat tggaagagtt taaaattgct tataaaaatg tgtaccataa gtatagtgaa
    32461 gtaaaaaaag ctttaaattg cttgacagaa aataaagatg agatgcaaat tcaacgagaa
    32521 atagatttat taagatttca aataaatgaa attgaagctg caaatctaaa taagaatgaa
    32581 tatgaagatt tattaaaaca aagagaagtt tatagaaata gtgaaaagat atataataat
    32641 ctaaattcga gttatagcaa gttacataat ggtgagtata atgttattga cctaataggt
    32701 cttgcatcta aggaacttaa tgacatatct aaatatgaca gtgttttaag tgaatatagt
    32761 gatactgtag aaagaatcat gtatgagtta caagacatat ctggtgagat aagaaattac
    32821 aaagataata tagactttga accttatgag ctagagcaga ttgagcttag aatcgacgag
    32881 attaataact taagaagaaa atatggagac agtatagatg atatatttga gtattatgga
    32941 aaaattaaag atagattaga tgaaatatta aacagagatg aaagagttga acagttaaga
    33001 agcaaactga tgaatataga agaagattta aaaataaaag ccagtaaatt gactaaagca
    33061 agaatagaag tagcaactac tttagaaaaa atacttcttg atgaattaaa aagcttaaat
    33121 atgaaaaatg taatgttcaa ggttaatttt gaaaaaggtc catttacttt agatggagta
    33181 gatgatattg aatttatgat atcttttaac ttaggagaag atattaagcc tatatataaa
    33241 gttgcatcag gtggtgagat gtccagattt atgttagcgt ttaaaaccat attggctgat
    33301 atagatgata ttgatacatt agtgtttgac gagatagata ctggaataag tgggatagca
    33361 gcacagatag ttggtgagaa attgagtgat atagcaaaga aaaaacagat aatatgtata
    33421 actcatttac ctcaaattgc tgcaaatgca gacactcatt attgtataga gaaagatact
    33481 tcaaataata ggacgtttac caatgttagt aagttaaatg aatctcaacg aaaaaatgaa
    33541 attgccagac ttatagcagg taacaatata acagaaaaaa ctattgaaca tgcaagtgaa
    33601 attattgagt tggctaaaaa atgttagtga aaaacaagca ttttaatagg tgtaaagaat
    33661 ttttatagaa atgctttaca tctatttttt atgatgaaat tattttaaaa tatctagtgc
    33721 ctatattgaa gaaaagtaga tttaaaaata gaagaaaatt tgtataaatt tgtaactttg
    33781 aaatttctaa atgcttaagt ataatattat ttattaaggt agtagtgagg gggacataaa
    33841 atgaataaaa aagatactat gcacttaatt tttacaattg taatttttat tttattatgg
    33901 tatattgtgg tagcagtaag tgagattaat aaaaatattg aaagttgtag taataaatta
    33961 gattatttgt cacaagagat tatgagtact aaatcggata ttagtaatac tataaatgaa
    34021 gaaatgtcaa aaagttatat aacaaagagt attgacttaa aagtaaaaaa aatcgagaaa
    34081 aaagagtgtg ttattgatgt agatgttcaa ttaagtaaac ttggaaaaaa tggaaaagta
    34141 ttttttatgt ataaagaaga tagtgataaa tggaatgaaa ctgagatgac aaaacatgga
    34201 gaattatcat atgcatgtga aattaagata aatacaggaa gtgaatatga ctataaagta
    34261 gttacaagtg gagctatatc tgaaagttct gatgtgcaaa aattgactaa atctgattat
    34321 atgcctgaac aacccatatt taattctggg ataagagata atagagaata ctttattgaa
    34381 atagtagaaa atagtaatct tttcaatcat gagagtgaaa agtcaaaaaa taaagtttat
    34441 gataatatta gattagaaaa agtagatatt attgtaaatg aaggaaaaaa agacactata
    34501 tacaaagcca aattagctga aggcttagat gatggcaaaa ctaataattc aaatagaaat
    34561 caagtgaatt atgaagtaag ttttcctaaa agggatattg atgacataat ttatattaaa
    34621 gctaaactta catacaataa tggagtagaa tatacaaagg atataactga agatataact
    34681 atgggattac aagatttaga aaaataaata catatttaaa taaatattca aatagatttg
    34741 ttcaataaaa tagagtataa ataaaactgt agtagtttta cttatactct attaaaaaat
    34801 tataatttta attccatata tctagcccct tcaattggat tataagtata aggttctata
    34861 tcataaaaac caaacttttc atataatcct tgtgcacttt tcatgcttgg aagagtatct
    34921 aatctcatgt gtgtatatcc aaatttttta gcttcttcaa tgatttcttc caatagtatt
    34981 tttcctattt ttaatcctct aaattgattt cttacatata atctttttaa ttcacatatt
    35041 ttaccttcaa gtttttttaa agcaacacaa cctgccaaat tttcatcaac aaatgccaat
    35101 atcaatgagc ctgaaggttt tttatatttt cctggaagtg tttttaattc attattaaag
    35161 tcttgaaaac ataagtctat atttagagaa tttgaatatt cagtaaatag aagttttacg
    35221 ttttcaaggt tttctaaatc tttttcttca acaaattgta aagttatatt ttgagccatt
    35281 ttatccgcca ttatataaac atgtttatat aatgacttca cctccttgat atatgtatat
    35341 ttttcttaat atcaattaga ttttaatacc attacttttt caccaccata gtcaagctct
    35401 ccattaaact caaatccaaa atctttataa agcttaatag ccattttatt gtcttcgaat
    35461 atactaagat atagttcatc acagttatat tcatttttaa gatgatttac aagaaaatct
    35521 aatgatgctt ttccatatcc tttaccttga tattttgaat caatcataaa tctatcaagc
    35581 caaactctat catcttctaa acgaccatac atagcaaatc ctattaaaga attttcatca
    35641 tatattccaa caggtaccca agctgttata aatttagatt cagcaataga aagtgcattg
    35701 ctttctatat aatctaactg acttttatct acagacaaac taactaaatc atgccaatta
    35761 tttatgtctg caattcttac atttaatttc aagatattac cgcccttcaa actttttgtt
    35821 acaatctaat actatcatat tatgtattaa aaatcactta ttaagatttt aaataaactt
    35881 tttaaatgaa taaattctca ggaaaaagga attataaata cataataata cagcatagga
    35941 gtagatggat atatgaacaa aataaataaa aatttcaaaa atatttggat aatattagga
    36001 gctgtatcga tttttaccat ggttataatt tttaataaaa attgtaatgt taataaagag
    36061 actcaaatag aagcttttac aattaagaat catgataaaa aatttgtata tcctatggga
    36121 aatataattg gagtaaaagc aaatacagat ggtgtattgg tcttaggata tgaggaagag
    36181 gatgtagact atattggagg catacaaatc ggtgataata tagtaaaaat agataataaa
    36241 aggataaaaa atagtcaaga tgtatcagag attttgaata agattaaaaa aagtaaagtt
    36301 gaagtgactt ttgagagaaa aaataagtat aaaacagaag taatagagac taaaaaagaa
    36361 aatggcaagt ataaacttgg attatgggta agagataaaa tatctggaat tggaactatg
    36421 actttttacg accctagtat ggaaaagttc aaagcaatcg gtcatgccat aaaagattcg
    36481 gatactaatg aactattaaa gataaagcaa ggatatatat ataagccaga acaattaaaa
    36541 atagtaaaag ctagtaatga aaaagtaggt aaaataaaag gagattttaa tgacagcaat
    36601 ttaatgggta atttctcaaa taattcagag ttaggtataa gtggtaatat tacagaaaac
    36661 cataataaag agtttaatgt agcaaataaa gaaaagcaat taatagaagt tggaagaccg
    36721 caagatgtaa aaataggaga tgcagtaatt ctttttgagg ataaaaacaa aaatataaca
    36781 agctatgata taaaaataga aagtatagta tatgataaag gaaattatag agatatggta
    36841 ataaaagtag tagatgacaa gttattggaa tacacaggag gtatcgtaca ggggatgagt
    36901 ggagctccaa taatacaaaa taataaaatt attggtgcaa taactcatgt ttttagagat
    36961 aatccgaaaa aaggatatgg tatttttata gatgaaatga taaaattgta ggtgaggcat
    37021 taaaaaattt tattatttta tcaattatct aggaggaata taattttgga gtgtcgaata
    37081 tgctttagag tagataatta ggaagcaatt gtgtaaaaag tttagttttc tgtaataaga
    37141 agatgttttt taatgggggg atttttagtg gaaaaaatca aaatagtttt agcagatgac
    37201 aataaggatt tttgtcaggt attaaaagag tatttgtcta atgaagatga tatcgatata
    37261 ttaggcatag ctaaggatgg aattgaagca ttagacttag taaaaaagac acaaccggac
    37321 ttattaatac tagatgtaat aatgccacat ctagatggat taggtgtaat tgaaaaattg
    37381 aatactatgg atatacctaa aatgccaaaa ataatagtac tatcagcagt aggtcaagat
    37441 aagataactc aaagcgcaat aaatctagga gcagattact acatagtaaa gccatttgat
    37501 tttgttgtgt ttataaacag aattagagag ctagtttcta atagagttac acaagttgag
    37561 ccaaaaccta gaccagttca agagactcaa atgactagaa gtgattttgt taagaatgta
    37621 ggaaatatag agacagaaat cacaaatata atacatgaaa taggagtacc agctcatata
    37681 aaaggatatt tatatttaag agaagcaata aaaatggtta ttgataatgt tgagctttta
    37741 ggtgcagtaa caaaagagtt atatccaagt atagctaaaa aattcaatac aacaccaagt
    37801 agagttgaaa gagcaataag acatgcaata gaggttgcat ggagtagagg aaaagttgac
    37861 acaataaatc aattatttgg atatacggta cacaatacta aaggaaaacc aactaattca
    37921 gaatttatag caatgattgc tgataaatta agactagaac atagtatggt taaataaaca
    37981 agacataaaa agtaaggctt tttaattaag gcattggcta taaatgcgta ttacaagcag
    38041 cgaaacggta taaccactag ggttataccg tttcgctatt ttaataaata taaaaatttt
    38101 ctttattatt tgcttactat atcaatataa taattttatt atactatgga tatagtatgt
    38161 gtctttacaa gttgtaaact gacagtggtt tattttttaa tataaatatt gactttgatg
    38221 caggtaaact ttgtattttt aagcgtatta tggaatatgt taaataaaaa aatgatgaaa
    38281 tatagtattg taaatgccaa agatgcaaaa caaacttaaa acatttattt tattgttaag
    38341 taatgctata atataatgtg attttaataa tgatagtgga ggtttaaata tgagagtcga
    38401 ggcccctata aaagtagatc gaaaaaccaa aaaacttgct aaaagagttg aaagtgggga
    38461 aatagcagtt ataaatcata tagacataga tgaagttgct gcaaactctt tagtagaagc
    38521 taaaataaaa cttgtcataa atgcggctcc ttctataagt ggtaggtatc ccaataaagg
    38581 tccagggata ttaactgaaa ataatatact aataattgat aatgttggag aagagttatt
    38641 tgaagaatta aaagaaggag aaactataga agttgtagat ggaaagatat atagaaaagg
    38701 aaaattttta ggtgcaggag aagttttaga caagtatgag gtttcacacc aaataaaggc
    38761 tgcctatgag aatttagctg tagaattgga tagatttata gataatacta tagattatgc
    38821 taaaaaagaa aaaggattta tacttggaga agtagaaatt ccaaaagtga aaactaatta
    38881 tgctaataaa catgtattga tagttgtaag aggtcaggac tacaaagaag atttaagtac
    38941 tatgctttct tatatagaag aggtaaagcc agtattggtt ggtgttgatg gaggagcaga
    39001 cgctttaatt gagtttggat atactccaga tgtgattgta ggtgatatgg atagtgtaag
    39061 tgatgaagct ttaaaaaagg ctagtgaaat agtagtacat gcttatacag atggaagagc
    39121 accaggtctt aaaagggttg aggaattagg cttagatgca gttgtatttc ctgcaccagg
    39181 aactagtgaa gatatagcta tgcttatcgc ttatgagtat aaggctgagt taatagtagc
    39241 agttggtaca cattcaaata tgattgattt tcttgaaaaa ggaagaaaag gtatggcaag
    39301 tactttttta gttagactaa aaataggttc aaagcttata gatgctaaag gcgttaattt
    39361 gttatacaga agtaaattaa aaataaagta catatgggca ttaatagcaa cagcattatt
    39421 tcctgtgttg gtagttgcat cattgtcacc aggagttcaa cagttcatac aactaatgca
    39481 attaaagttt agagtattat tacagatgta aattttagga ggataaatat gcatattaat
    39541 atgaaatatt atatagtcac tataggggct atatttatag ctttgggtat aggcatgtta
    39601 gtaggattta atttaaataa taatcaacaa ttaagtgaac aacaagcaaa tataataaat
    39661 gatttggatg ataagtttga tatcttaaaa gaaaaaaatg ataaacttga cagtgactta
    39721 gcaagtgtaa atagagatta tgaagaggca gttaatttta taaataaaaa tgtagataag
    39781 attttagctg gttcgctgaa tggtaagagt ataggaataa tatctgctaa tgaaaatgac
    39841 gattatacaa aaaatataga agacataata aataaatcta atggaagcat tgcatttaat
    39901 ataatattaa aagaaaacat aacaaatcca gaaaagttaa aggaaatgtc aactaaacta
    39961 ggtacagatg taaaaaatgc aaatgatgca gttaattata taattgacac tcttaaaaag
    40021 gaagatgcaa gtgacatttt aacatatctt caagaactag atgtaataaa gtttaatttt
    40081 attggagata catatctaaa atatgattcc gttgtaatag ctggtggaaa tgatgcaaaa
    40141 gacagtgcaa aacagtttga aaaaatagaa aaatttgtag tttcaaaact taaatcagag
    40201 aataaatatt tagtagaagt tcaaaataca ggagttaaaa cttcttatgt agaactatat
    40261 tctaaaaaca aagttgcaac tatagacaat attgatgaag gtataggaag tatatctcta
    40321 gcgattcttc tacaacaagg taatatagtt ggtaattttg gtagattgga tactgcaact
    40381 tcattgttgc catctattaa atagaaataa ttttaaggag agaaaaaatg gattcttata
    40441 taagtataat aataccagct tataatgaag aaagtagaat aaaatataca ttagaaagca
    40501 tattggacat taagcaaata aatgaaataa tagttgtgga tgatggttca attgacaata
    40561 ctgctaaggt tgtatctgag ctagaaaatg aaaagattaa attttttaag ttagataaaa
    40621 atagaggtaa aggatatgct ttaaattatg gtatcaaatt agcaatgaaa aatgcagata
    40681 ttataggttt tctggatgga gatttaggaa gttctgctaa agaagttgaa aaattaatag
    40741 tacctatatt aaatggtgag gcggatgtta caatagccaa gtttcctcct gcaaagagaa
    40801 aaggaggttt gggatttgta aaaggtttag ctaaaaattc tgtgtttgag atgactggtg
    40861 ttgagttaga tgcaactttg tcaggtcaaa gaatatttaa aagagaagtt ttagaaaagt
    40921 ttgatgaaat accttttgga tatggagtag aagttggaat gaccattgat atattaaagt
    40981 atggatttaa aattaaagaa gtattagtaa atatgactca taatgaaaca ggaagaaatt
    41041 taaaaggttt tattcataga gggaaacagt attatcacat aaaaaaggtt ttaggacaaa
    41101 aaaagaaaga atggaggtaa tatcttagtg aaagattata tactttatac cattttatta
    41161 ttaactggat tatttggaac ctatgctgta attccactat ttaaaaattt attgataaat
    41221 ggaaatgtac ttagaccaaa ctataaaaaa gatatgatac cagtaagtat gggaattgta
    41281 tttttgccta tgattgtaat caatggaata atacttgctt tctttactac agaatttaag
    41341 gatttagcgt atatatttat gtttatgttt ggtatgatat ccatgttttt tgcagggata
    41401 ctagatgatg tcattggaaa tagagatgta agtggtttaa aggggcattt taaaagttta
    41461 tttaaaggaa gtcttacaac aggaggtttt aaagctttat ttggtggatt tgtagggctt
    41521 atagtttctg ttgctatatc aaaagacata attgatataa tagtaaatac actaataata
    41581 gctctatcaa ctaatttaat gaatcttttg gatttaagac caggaagggc aataaaagca
    41641 tatttattca taatggtaat tatattttta acattgacag gatttgttca agttcttcca
    41701 ttacttatag taccaaatgt acttgcatac tttaattatg atttaaaagc aagggctatg
    41761 atgggagata ctggttcaaa cgttcttgga atatctattg gaatgctcat ttcttttggg
    41821 tatccattta acattagatt aggatggtta atatttgtag ttattatgca tatattcact
    41881 gagaaatact cattaactaa gataatagaa aaaaataaac tattaaattt tatagataaa
    41941 ttgggaaggt agtattatga taagcaaaag agtggggatt gttgaatcaa tagtaagtca
    42001 aactgaaact ctagatgata taagagtaaa tataaatgga gaaatacaaa aggcatataa
    42061 ttatccaaaa atatctggaa ctataaatat aggtgacgaa gtggttttaa atacaacagc
    42121 agtagaactg agcttaggta caggtggata ccattttgta ataactaatt taaataatat
    42181 agaatctact ttaactgaag gtggacatat aatgaaactt agatatacgc ctcttcaaat
    42241 aaaagtggat tctgttgagg aacaagaaag tatttatcat gataaatttg cgaattttaa
    42301 aggattagaa ggtctttctg ttgtagtagg gactcttcat agtatgttaa ctccatttgc
    42361 agcttcattt aaaagaaata atccaaataa aaaattagtg tatataatga ctgacggagc
    42421 atctcttcca atttatttaa gtaaaaatgt ggatacttta aaagaaaaaa aattaataga
    42481 tagtacaata actataggaa atgcgtttgg tggagattat gaatgtataa atatatatac
    42541 tgcattaatt acagcaaaag aaatactaaa agcggatgca gtatttgtca gtatgggacc
    42601 tggaattgct gggacaggaa caaaatatgg atttacaggg attgaacaag gctctattct
    42661 tgatgccgta aaaaaacttg aaggaagagc aattgcaatt ccaagaatta gttttgctga
    42721 caaacgagaa agacatcaag gtatttcaca tcatagtatg actgtattta aagaaattgt
    42781 aaatgtaaat gtagacatac caataactat atatgatgat gaaaaattga actttataaa
    42841 agagcaaatc aatttaaatg gtttagacaa aaagcataat ataatttata tagataataa
    42901 taaaacgaaa gaggatttag actattttaa tctaaaagta aagagtatgg gaagaaatta
    42961 tgaacaagat gaggcatttt tcaaagctgc aagcacagca gcatattact taatggaggt
    43021 ttaagatggt attagaagaa aaaactatta gtagtgatag ggtttataca ggtaaagtaa
    43081 ttactttaaa ggttgatact gttgaaattc cagggcaagg atatcaaaaa agggagttag
    43141 tagaagttgg tggagcagta ggaatagttg caatcacaga tgataataaa gttgtgttag
    43201 ttaagcaatt tagaaaacct atagaaaagc caatctttga aattccagct ggtaaactag
    43261 aaaaaaatga aagtccaaaa gaatgtgctg aaagagaatt aaaagaagaa actgggtata
    43321 gtgcaaaaaa tattaagcta atacataaat ttttcacttc agcaggtttt tctaatgaga
    43381 taatgtttgt atatttagca acaggtctta ctccaggaga gaataattta gatgcagatg
    43441 agtttttaga tgtctatgaa attgagcttg aagaagcata taacatggta ttaaaaaatg
    43501 acgttgaaga tgcaaaaaca tctattggat tattattagt aaaagatatg tttaaaaatt
    43561 aatatttata aaatgaataa ccctttgtcc taaagcatat tattttataa aggacaaggg
    43621 ggaattaaat tgagaagaac atatagaaaa aatgatttta gagaattaaa tagacaaata
    43681 attattatag gattgttgtt tatgatgtct attgttgttg ggtcttacat aaataaaata
    43741 ttgccaggtt ctagtaacaa tatattaaac aatataaatc ctgctgtaga gtattataat
    43801 ctaaatataa gcataaaaga tacagtgata caaaatttaa aatcagatgc aatctttatg
    43861 ggctcaatag ctctattaag tctatttgta gtaacaattc cagcagtgtt ggtagctttt
    43921 gtattaaaag ggatgtcaat tggttataca ataaatagtt gtatattggc attaaaattt
    43981 aaaagtataa aaatgatttt aataatatta tttaaaaatt tgattattat tccatgtgca
    44041 atcatattag cattaattag tttaagttat tttaaagaaa tggtttatga atttaaaaag
    44101 aaaaatagaa aaaatatgca atttttaatt aagaggtata tactaaatat tataattata
    44161 atagcactat cattggggtt gcaattaata ttaaatactg caagtattgg tattattaag
    44221 ttcctagcta aataaaggag tttataat
//
LOCUS       DAELNY010000012        43895 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.012, whole genome shotgun sequence.
ACCESSION   DAELNY010000012 DAELNY010000000
VERSION     DAELNY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.012"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            130..480
                     /locus_tag="KPE55_000568"
     CDS             130..480
                     /locus_tag="KPE55_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0491669.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            499..2142
                     /locus_tag="KPE55_000569"
     CDS             499..2142
                     /locus_tag="KPE55_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG0491670.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2223..4280
                     /gene="recG"
                     /locus_tag="KPE55_000570"
     CDS             2223..4280
                     /gene="recG"
                     /locus_tag="KPE55_000570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG0491671.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4339..4893
                     /gene="rsmD"
                     /locus_tag="KPE55_000571"
     CDS             4339..4893
                     /gene="rsmD"
                     /locus_tag="KPE55_000571"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG0491672.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4895..5392
                     /gene="coaD"
                     /locus_tag="KPE55_000572"
     CDS             4895..5392
                     /gene="coaD"
                     /locus_tag="KPE55_000572"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG0491673.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5396..5920
                     /locus_tag="KPE55_000573"
     CDS             5396..5920
                     /locus_tag="KPE55_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491674.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6264..8183
                     /locus_tag="KPE55_000574"
     CDS             6264..8183
                     /locus_tag="KPE55_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0491675.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8196..8483
                     /locus_tag="KPE55_000575"
     CDS             8196..8483
                     /locus_tag="KPE55_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0491676.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8616..9875
                     /locus_tag="KPE55_000576"
     CDS             8616..9875
                     /locus_tag="KPE55_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG0491677.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9878..10618
                     /locus_tag="KPE55_000577"
     CDS             9878..10618
                     /locus_tag="KPE55_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0491678.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10941..12251
                     /locus_tag="KPE55_000578"
     CDS             10941..12251
                     /locus_tag="KPE55_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0491679.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12349..13266
                     /locus_tag="KPE55_000579"
     CDS             12349..13266
                     /locus_tag="KPE55_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0491680.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            13282..14091
                     /locus_tag="KPE55_000580"
     CDS             13282..14091
                     /locus_tag="KPE55_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0491681.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14149..15897
                     /locus_tag="KPE55_000581"
     CDS             14149..15897
                     /locus_tag="KPE55_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG0491682.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16159..17181
                     /locus_tag="KPE55_000582"
     CDS             16159..17181
                     /locus_tag="KPE55_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG0491683.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17186..19492
                     /locus_tag="KPE55_000583"
     CDS             17186..19492
                     /locus_tag="KPE55_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0491684.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            19464..20579
                     /locus_tag="KPE55_000584"
     CDS             19464..20579
                     /locus_tag="KPE55_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491685.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20597..22015
                     /locus_tag="KPE55_000585"
     CDS             20597..22015
                     /locus_tag="KPE55_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG0491686.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22030..23238
                     /locus_tag="KPE55_000586"
     CDS             22030..23238
                     /locus_tag="KPE55_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491687.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23312..24700)
                     /locus_tag="KPE55_000587"
     CDS             complement(23312..24700)
                     /locus_tag="KPE55_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0491688.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25041..26375
                     /locus_tag="KPE55_000588"
     CDS             25041..26375
                     /locus_tag="KPE55_000588"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG0491689.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26517..27434)
                     /locus_tag="KPE55_000589"
     CDS             complement(26517..27434)
                     /locus_tag="KPE55_000589"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG0491690.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27642..28526
                     /locus_tag="KPE55_000590"
     CDS             27642..28526
                     /locus_tag="KPE55_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG0491691.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28739..30580
                     /locus_tag="KPE55_000591"
     CDS             28739..30580
                     /locus_tag="KPE55_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG0491692.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKTEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30885..31592
                     /locus_tag="KPE55_000592"
     CDS             30885..31592
                     /locus_tag="KPE55_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491693.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31586..32896
                     /locus_tag="KPE55_000593"
     CDS             31586..32896
                     /locus_tag="KPE55_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0491694.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33013..33696
                     /locus_tag="KPE55_000594"
     CDS             33013..33696
                     /locus_tag="KPE55_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0491695.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33689..36076
                     /locus_tag="KPE55_000595"
     CDS             33689..36076
                     /locus_tag="KPE55_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0491696.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36356..37498
                     /locus_tag="KPE55_000596"
     CDS             36356..37498
                     /locus_tag="KPE55_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0491697.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37517..38041
                     /locus_tag="KPE55_000597"
     CDS             37517..38041
                     /locus_tag="KPE55_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0491698.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIDRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38141..38959
                     /locus_tag="KPE55_000598"
     CDS             38141..38959
                     /locus_tag="KPE55_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0491699.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39037..39348
                     /locus_tag="KPE55_000599"
     CDS             39037..39348
                     /locus_tag="KPE55_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG0491700.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39525..40208)
                     /locus_tag="KPE55_000600"
     CDS             complement(39525..40208)
                     /locus_tag="KPE55_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491701.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40301..40942)
                     /locus_tag="KPE55_000601"
     CDS             complement(40301..40942)
                     /locus_tag="KPE55_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491702.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41116..43119)
                     /locus_tag="KPE55_000602"
     CDS             complement(41116..43119)
                     /locus_tag="KPE55_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG0491703.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43272..>43895)
                     /gene="recX"
                     /locus_tag="KPE55_000603"
     CDS             complement(43272..>43895)
                     /gene="recX"
                     /locus_tag="KPE55_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG0491704.1"
                     /translation="KIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEINEEE
                     LKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSYNLVN
                     DSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENAMYLAK
                     KRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        17082 a         4802 c         7555 g        14456 t
ORIGIN      
        1 ttttctaggt ttaaattata ccatatttat agtatgatat gattataatt ttatataata
       61 aataagtaaa aatagtaaaa aataaattaa gaaatgtttt taataaaaat aagcttaagg
      121 gggtcaacca tgagtgcaaa ggtaacgaat caatatggaa atatagaaat agataatcaa
      181 gtaatagctc aagtcgtata tagagctgca atggaaagtt atggcttagt aggtttagca
      241 tataagtcta aaggaatagt agagctatta aaaggcgaga acgcaactaa aggtgtaaga
      301 gttgaggaag tagaagaaga tgccatagca atcgaacttt atgttataat acaatatggt
      361 acaaatatat ctactgttgc caataatata atagatagag taaaatatgt agttgaaaaa
      421 atgactggcg taagagttac tagaattgat attaatgtac aaggaataag agttaagtaa
      481 tttcatagga ggaaacaaat gattcaatat atagatggta aaaggttaag ggagatgttt
      541 atttcaggtg caaacaatct ccaaaacaat aaagagttag ttgacaagtt aaatgtattc
      601 cctgtaccag atggagatac aggaactaat atgtcattaa caatatctta tgccttaaaa
      661 gaattagcaa aagttgaaaa tgataatata tcagatatcg gaaaagcatt atctaaaggt
      721 tctttaatgg gagctagagg aaactcagga gttatacttt cacaaataat cagaggtata
      781 gctaaatcta tagaaggaaa aagtaaatta tcaacagaag atttagcaaa agcttttaaa
      841 aatggttcag acacagcata taaagctgta ataaaaccaa ttgaaggaac tatacttact
      901 gtagttagag aaagtggaga gtttgcaata aaaactgcga aaaaagagaa agatgtagta
      961 aagtttttaa gtatgcttgt gaaggaatca aattcttcat tagaaagaac tccagacctt
     1021 ctaaagaatc taaaagaagc tggtgtggtt gactctggtg gaaagggtct tgtacttata
     1081 tatgaaggaa tgttagcttc tataaaaggc gataacatag aaataaaaaa tgttgaccta
     1141 gatactaata tttcaacaag tatggatttt gcaaagagta ctactagcac agatgatata
     1201 aaatattgct attgtacaga atttatttta gaaagttcta aggtagaaga tactaaaata
     1261 agagacataa tgatggctta tggtgatagc ctagcagtag ttggagatga tggagtcata
     1321 aaggtacatg tacacactaa tgacccagga aatgtacttc aagaagctct aaagtacggt
     1381 caattattaa ctataaaaat agaaaatatg aagttacaac atgaaaatac attactggat
     1441 gtagaagaaa agaaagaaaa tgatagtgaa cctttagaag aagaaaaaga gtttgggttt
     1501 atagctactt ctatgggtga aggtttggca aatatattta aagatttcgg agttgaccat
     1561 ataatagaag gtggtcaaac tatgaatcca agtactgaag attttatgaa tgctataaag
     1621 catataaatg ctaaaaatat atttattttc ccaaataaca gcaatataat aatggctgct
     1681 aatcaggcaa aagaattgag tgataaaaat ataatagtta tacctacaaa aaatacgcct
     1741 caaggatttg ctgcattagt aacttttaat ggagaattaa gcgaagatga aaataaagaa
     1801 gctatgatga atgctttaaa ctcagttaaa tcaggtcaag taacttttgc tgttagggat
     1861 acagtgatga atgaaattga tgttaaagaa ggtaatataa ttggaattgc agaaggaaat
     1921 ttattatcag caggagatta tgtggatgaa gtaacatcaa accttattga aaagttggtt
     1981 gatgaagata ctgctataat aacattattc tttggtgaag atgttacaga atctcaagca
     2041 aatgaactac gtacttcttt agaagaaaag tttgaagatg tagatgtaga gttatattat
     2101 ggaggacaac ctctttatta ttacttaatt tcagttgaat aatgattata agaacctgta
     2161 tgaccaagta gcttttataa aattggttgt acaggatttt tgtgtagtag gtgatatatt
     2221 tgttggattt atataaagat gttcaatatg ttaagggaat aggacctaaa aaagcagata
     2281 aattaaataa actgggtata tttacactaa aagacctatt gtattatttt ccaagacaat
     2341 ttgaagatag aaataatcta aagaaaatag cacaattaga agatggtgag aaagttacga
     2401 taaaagcagt aatatcaagt ataaatacat ttagcccaaa agaaggtatg actttaacaa
     2461 agattgatgt aaaagatgag acaggctcgg ctaaattagt gttttttaat aagagttata
     2521 taaaaaatac ttttcgtcca ggagattcaa tattagtttt tgggaaggtt aagaaaaaat
     2581 ttaataactt agaacttaca tcatgtgaat tagaatatct tactaattca cctaagaata
     2641 catgtagatt tatgcctgtg taccagctta catacggtgt tacaaataaa gaaattatga
     2701 gtataattag aacagtactt gaagataaag aattaattat acaagaatat atgccacaaa
     2761 gaattataga aaaatatagg ctatgtagta tagattttgc agttagaaac atacattcac
     2821 caagtagtaa ggaatcatta aaaatagctt tatatagaat tgtatttgaa gaattactta
     2881 tactacaact agggttgttt gtatttaaaa gtggcagaaa taaagaagat ggaataaagt
     2941 ttgaaacaag taaagactta aaaaaaataa taagtgcttt accatttaaa ctgactaaag
     3001 ctcaaaacag ggcattagat gaaattatac aagatatgaa tttagagaaa ataatgaata
     3061 gattggtaca aggtgatgta gggagtggta aaacagtagt agctctctta gcacttgcaa
     3121 attgtgtttt aaatggttat cagggcgctt tgatggcacc tacagagata ttagcagggc
     3181 aacattatat ttcacttact gagtctctaa aagattttgg aataaatgta ggtctattaa
     3241 taggtagtct tacaaaaaaa caaaaggata cagtattgga acaaataaag aataatgaga
     3301 ttgatatatt aataggtaca catgcactaa ttgaagataa ggttgaattt aataatattg
     3361 gtttagttat aacagatgaa caacatagat ttggtgttat gcaaaggagc aaactttcat
     3421 tgaaaggagc aaatcctgat atattagtaa tgacagcaac accaatacca agaactttag
     3481 cacttatact ctatggagat ttagatattt ctattataga tgaacttcct ccaggaagac
     3541 agccaataga aacaattgct attgaaaaaa gtaagcgaga tagagcatat aataatcttg
     3601 taagaagaga ggttgaatca ggaagacaag tgtacattgt atgtcccttg gtagaagaaa
     3661 gtgaagccat agaagccaaa tcagcagttg aacttgttga ggagttaaga gcggaatatt
     3721 ttcacgactt aaggcttggg ttgcttcatg gtaaaatgaa gtcaagtgaa aaagatgaag
     3781 taatgagact ttttaaagat aaagaaatag atattttagt ttcaacaaca gtaatagaag
     3841 ttggtgtcaa tgtgccaaat gcaacattaa tgataataga aaatgcagaa agatttggac
     3901 ttgcacaatt acatcagctt agagggcgag ttggaagagg tagtcataaa tcgtattgtg
     3961 tactaattta tgattctaaa acagatgtat gtagacaaag aatggctata atggaagaga
     4021 ccaatgatgg ttttaagatt tcagagaagg atttagaaat aagaggacct ggggaatttt
     4081 ttgggacaag gcagcatggt cttccagaac ttaaggtagc aaacttattt aagcatataa
     4141 aaatactgaa gttagcacag caagaagctc gatatatatt gggtgaagat aataatctac
     4201 aattaaaaga aaacatggca ctcaaaaaag aaattataga taaatttaaa gatacattga
     4261 aagaaatttc attaaattaa ttttaaattg tgttaaaata ttactaatgt aataatgtgc
     4321 tgaagggaga ttgtcatatt gagagtaatt tcaggaaaag cgagaggatt aaaactaaat
     4381 actccaaaaa atgaagatgt aaggccaact acagatagag taaaagagtc tctatttaat
     4441 atgataaatt catatataat ggaaagtgaa gttctggatt tatttgctgg gactggttct
     4501 ttaggaatag agtgcttatc taggggtgcc aagtcatgta cttttgtaga tattagtaag
     4561 gaaagcatag atattgttaa gtccaatata aaaaaggcaa gagttgaaag tgaaagtatt
     4621 attttaaacc ttgattttaa gactgcaata gataaactta aattacaaaa tagtaaattt
     4681 gacataattt ttatggaccc tccgtattat aaaaatatgt ttatagaagc tattgaaaaa
     4741 attgataatt ctaatctatt gaacgaagat ggaataatag ttgtagaaca tgatacgaat
     4801 gatttatttc cagataagat atgtaaattg gagaaaacta gagataagaa atatggaaat
     4861 actacattaa ctttttataa gatggaggca taatatggaa aataaaccta gaaaagctat
     4921 atttgcagga agttttgacc caattactaa tggacattta gatattattt gcagggcttc
     4981 gaagttattt gacgaattac aaataggagt attaaataat cctaataaaa agggtctatt
     5041 tagctttgat gaaagagtaa aacttataga aaaaagtaca agccatttaa ataatataaa
     5101 agttgtgact tttgatggtt tattaataaa ttactgtcag gaaaatggga ttggagcatt
     5161 agttagaggt gtgagaagtg gtgctgatgt tgattatgaa cttcagatgg cacatatgaa
     5221 tagagagctt aatccagata tagagactat aattttacca agttgtacaa aatattcatt
     5281 tataagttca tctttaataa aagaggtttt gctctttgat gctgatataa aaaatttggt
     5341 tccaaagatt gttttggaag aattaaagaa aaaaactagt gggggtaatt gatatatgaa
     5401 gatagattta gaaatgattg aactatttga gcagttagag gagatattta gaagtgcttc
     5461 tacaatccct ttctcacata aatgcacagt ggataaagat gaggttttag caataattaa
     5521 tgatataaga actttaatac cagaagaagt aacacaggca gtatggatta ataaagaaag
     5581 aaataaaata ataagtcaag ctaagcaaga tgctacaaat atagttgaac aagcgaaaaa
     5641 agaagctgag agaatacaac aagaatatca agataatata gaggaattaa agaaaaattc
     5701 tgaagatgta gtaaaggcat atgtagaatc cagtgaacct gttgttcaag cagaccaaag
     5761 agcaaaagat atagttgata gagctgagag aatagccaat gagataagaa ttgggtcaat
     5821 tgaatatgca gaggatgtgc tttctagtgt tgagtataat ttaagagaaa tattagaaga
     5881 agtaaaaaga gataaagctg aacttagacc taaaaaataa attttaaata aataaaaatg
     5941 gagctatcac acaaaaaatt ccaatttatg tgtgatagct ctcttttaat atagcaattt
     6001 tttgttatat aattgtgctt tttagattac ttatatctta ttttgatatt ataatgtagt
     6061 ttaatctttt atttgttgaa atcaacttat ttatttgtat aaataatata ggttattttc
     6121 aaattttttt aataatttta ccactgtctt cttttagatt agagttagac tgtgcaaaat
     6181 tataaaataa tactaaatca tgtgcaatta tttggtatgg cactacatgt agaaaatata
     6241 gataataaaa ttaaggaggt agtatgaagg aaagggctaa aaaaatatta gattttatta
     6301 ttaaaaataa caatgtaaca tcacaagaac tacaagaaaa gtttaatgtt tcaaagagaa
     6361 ctatttatta cgatattcta gctataaatg aacaactagg aaaaagtggt aatataaaaa
     6421 atgtaaaaca caaatttata tttgaaggta atttatgtga tgcaaggaaa ataatttcaa
     6481 ctgaagaaga taaattttta gatagtgatt acagaaaaac ttttatatta aacaagatat
     6541 tacttggcga aaaaatatca atagaaaaac taacaaatga aatgttactt tcaaaaaaca
     6601 cagtagtaca aacaataact gatgtaaaaa aataccttca aacaatgggg ttaagattag
     6661 agtataaggg aaagtacaaa attataggtg atgaatacgt tattagagag ctgtttctga
     6721 taattgttca agaaaatata ttggaaatta attctataag tgaggaagta agtagtttcg
     6781 atacaaaagg acatataaaa cttactgatt attctttatt aaatctaact aagtttgtgg
     6841 aatttctaaa taaaaggata agagatggga aaacattata ctcatataaa tatctaaatg
     6901 aagctaaaaa aattagttac tttagtaatt gtaaagaact tttatgtgaa gaagctaatg
     6961 aaaatgaaca agcctatata tgcacttaca taagttcact tcccagctta aatagtgaag
     7021 ttaaggaaga tgttgttgaa gaatatgtag ataaacttat agataagttt gaagtaaata
     7081 cagcaataaa attagaaagt aagcatgagt ttaagaaaaa tattttaagg cacctccatt
     7141 cttcttacaa cagaatacga tttaagtttc ctattagaaa tccaatgtta gatgagacta
     7201 agtataaaca tgagtcactt tataaaatta taaaatcaat tatagaaaat gaagaagagt
     7261 ttcctgtatt tgaggggata agagaagaag aaataggatt tatagcagcg tattttggag
     7321 gatatttaag agggtctagg gataatggtc taagaagaaa taaagtctta ctagtttgtc
     7381 ctaatggtct tatggtatca aagagtttgg aaatacaact atataaatac ataccaacca
     7441 tagaaatagt aggtatagta tccattaagc aactaaaaga agtaaatgta tactacgatt
     7501 acataattac aactatagat atacaaaatg taaacaacgt aattgttgta aatcctcttt
     7561 taacatcatc agatgttcaa ttacttatga acaaactaat aagtgtaaaa gaaaacgaga
     7621 agtatttcaa tcttgaatta ataatacaag ctataagaaa aaatggagtt atcaataatg
     7681 aagaagcact aaaagctgat ttattaaaca taatacacaa gatagatgaa ggagagatgt
     7741 atcaaccaat gttaaaggaa ttaattaatg cagaaagggt caatataata aaaagtgtta
     7801 gagattggaa agaagcaata aaaatagcct caaagccatt gttagaagat aactctatag
     7861 aagagttata tatagaaaat atgataaagt cagttgagaa atatggacca tatattgttt
     7921 tagctgatag atttgctcta ccacatgcaa gttctaaaga aggagtaaac aagcttgcta
     7981 tgtctttact aatagtagaa gatgaagttg atttattagg aaaaccagta aatatattta
     8041 tggtattagc tgctgtagat aacactactc acattagagc attagcatca ctatcagaga
     8101 tgatgtacga ggaagaaaat gtaaaactaa taattaatgg tgataaaagt tcaatactag
     8161 aacttataaa taagcaaaat taggaggaat gaaaaatgaa gatattagca gtatgtggtt
     8221 ttggtgtagg ttcatcaatg gtattaaaaa tgacaataca aaaggctcta aagcagttag
     8281 gtatagaatg tgaagtagaa aatacagata taaattcagc tagaggtgct gattgtgact
     8341 gtatattcac aagtcaagaa ttgttaaatg aattaaaagg aacttgtaat gtagatgtat
     8401 atccagttaa aaagtacatg gatttagcag aagtcacaga agttgttcaa acttttttaa
     8461 gtgataaaaa tgataagaaa tagtataagt tttaaaaagt aaatttaagt taatcagaat
     8521 aaaataaaaa cttactaaat tcaaaaatag taagaaagag aggagggatt tagtggtaga
     8581 aaacttaaac agttgaccta tcactaaaaa atataatgct tgattttatt tcaaataata
     8641 ttttacgtaa tccatctatg ttaattggat taatagcagc atttggtctt gtactacaga
     8701 agaagaaagc atctgatgtt ataaaggggt cactacttgc agctttcgga attataatta
     8761 tggagactgg aacaggtatg ttagttagtt caatagcacc aattaatgga gctttccaaa
     8821 gtataagtgg tggtgcagta acaaaaggtt taagtgatgt tacttttact gcaagttatg
     8881 gtggaattat aggtttagca atgtttgcgg gtttagctat tcacctaatg atagcaagat
     8941 ttactcctat taagacaatc tttttaacag gtcacatgct ttattggttc ccattcatat
     9001 ttgtagcttc aggagttgaa gctggattaa atggtggagt attaatagct tttgcagcag
     9061 tattatcagc actctattgg tcaataatgc cttggttatt aagaaaatat gtgtttgatg
     9121 ttataggaga tgattctttt acattaggtc atccatctgg ttgtttagca cttatatcag
     9181 gatttgttgc taaattagta ggaaataaag aaaaatctac agaagatata aacatacctc
     9241 aaagtttatc attttttaga gaaatatcta taagtggtgg aatggcagtg tttttaatgt
     9301 ttatagttgt tgggatagta ataccaggtg cgtttaaaga tagtaatgag tttattggaa
     9361 cgtatgcaat aaagcaaggt ctttcttttg gagcaggtct tataataatg cttcatgggg
     9421 taagaatgct aataaatcaa ataatgccag catttcaagg tatatctgaa aaagttgtgc
     9481 caaatgcact acctgctttt gactgtccaa tattatttaa ttataaacca aatgctgtat
     9541 taatgggatt cttagttgct atgctaacat ctacagctat gattttaata tgtaactact
     9601 ttaatgtatt taatttgcta cttataccac ttgttataac ttcattcttt gagtgtggag
     9661 cagcagcagt aataggagaa tcacaaggtg gtatacgtgg agctataata ggtacagcta
     9721 tagctgcaat atttatggta gttttagtag gaatatctgc tgtaatttac tcaggaacta
     9781 ttcaaaattg gatactaata tttggaggta atgacctttc attatttggt ataatcagta
     9841 ggtttatagg ttcactattt ggaggaatat tatagatatg aaatttaaat acatagatga
     9901 gataagaaga ttaattgata taatagaaaa cgaagaaaaa gtcaatatgg aaaaagcagt
     9961 acaaatcttt gttgaagcaa tagaaagtaa aaatgctata ttttcatttg gagcttcaca
    10021 tgcaggaata ttaacagaag aattattcta tagagcaggt ggctgtgttc ttataaatcc
    10081 agtatttgca aaatctctaa tgttagatac agcgcctata actcacacaa gtaagatgga
    10141 aagattagaa ggatatggta cagctctagc tgataaaact ccaattaaaa aaggagatgt
    10201 agttatagtt cattcagtat ctggtagaaa tccagtaagt atagagtttg ctatgaaagc
    10261 taaggaaaat aaggcaatag taatatgtat aactaattta agttactcaa aaagtgtatc
    10321 ttcaagacat tcttcaggaa aaaaacttta tgaagtatgc gatttagtga tagataatca
    10381 tggtcaaaag ggagatgcct gtgtagagat agatggatta aaccaaaaag ttagtccaac
    10441 ttctactgta atagcaacta ctgtgatgaa ctctattata gctcaagcta ctcaggaatt
    10501 agttaataaa gggctaaaga atcctccgat tttctatagt gcaaatattg atggtggaga
    10561 tgagttaaat aagaagattt ttgatgaata taaaagtgta attcattatg aatattaagg
    10621 ttaaaaaaat atgttaatga actttgaagt ttatcaacaa agaatattaa taatatataa
    10681 taaatgctat catttaaaag ctgtaggtaa aaatctacag ctttttatta ttgtagatta
    10741 aaaagatgcg taattaaatt ttaaattgga gaaaataaat tggaaggtat caattataaa
    10801 tattagaaac gttgtcatag aaaaaaacta tattaaaaaa aagtacatag aaaaaactga
    10861 ttagacaata aatacatgct ttgataatat tttaaatgga gtgaaaatgt gtagaattat
    10921 aatgaaaaaa gaaaataaaa atgaaaaaag aaaataaaat ggagggaata aaagtgggta
    10981 tgttaaaaaa attatgttca ctagctatgg tagcaattat gactacaaca cttataacag
    11041 ggtgttcaag tggaaatgga aaagattcaa aaaaggatgg cgaaaaagaa aaactagttc
    11101 tttggatgcc accagtagaa aataatatga aggaagtttg ggaccctatt ttagataaat
    11161 ttgaggaaaa aaataactgt gaagtagatt tacagataat tccttgggat aattattctg
    11221 aaaaatttgc aacagctata agtgctggcg aaggtccaga tgtaggatat atgtatgcag
    11281 aaatgtatcc acagtttata gaaagtggag cagtagaaga tttgactaat tatgcaacta
    11341 aagaggataa agagcaatct atttatataa aaacatctga aatgatgggt ggaatgtatg
    11401 gtatgccatt ccaagcagca aatccagggg tattatacta taacaaggat attttagaga
    11461 aattaggaga aaaacctcct aaaacatggg aagattttaa aagaatttgt aaaaaagcaa
    11521 cacaagatac tgatggtgat ggaaagatag accaatgggg attagctcag ggatggggag
    11581 ctaaaacttt tggtaactta aactggaact ggtatcctta tttatggcaa gctggtggag
    11641 atatctttaa tgatgattta aaatcggtta aatttaatga taagagtggt ttagaagcag
    11701 ctaatttctt aaaagaatta caagcatatg tgcctgaaga ttctttatct aaagatagta
    11761 atgaaatgat agaaagtgta tttggaccag ggaaagcagc atttacaata atgttatctt
    11821 ctgctgcaac aagtgtattt gatgaatcat tcccggattt aaattgggga tttgtaactg
    11881 gactagaaga taagaaagca gcaacttttg gagcagtaga ccatttatct ttaatgtcat
    11941 ctgctaaaaa taaagaactt gcatacaagt taatacaaca tatgttaagt gttgaatcaa
    12001 tgacagaatt tcataaagca ataccaagag caccaataac aaaaggtgaa ccatatcaag
    12061 gtgatgaaag attcaaagaa atggtggaaa atgacaaaaa tgtatataga ccattagtag
    12121 taggacctca tggagttgaa atatatgaat atttatggaa agaacttcaa actatgattt
    12181 caggagataa aactccaaaa caagcgttag atgatgctgc aaaatattca aatgatttat
    12241 tagcacaata aaagtttaaa gctgtaatat gttgtaaagg actttacaga ttaaaattag
    12301 cattgtattt ataaatacaa tgctaatatt tatattggag aggataaaat ggaaaactta
    12361 gttagagagt ttgaagaaaa ttcaaaaaga aaaaaaacaa aattaaatat taaaccatat
    12421 atgtatatat tgccattggg aataatttta gtggcgtttt atgtgatacc aataataatg
    12481 tcaatatact ttagttttac aaagtataat attattagtc cagctacttt tataggttta
    12541 gaaaattata aaaaattatt tacagatgaa acattaaaag tatctattat aaatactatt
    12601 aaatttactg ttgttgtagt accttgccaa actatattat cattgatttt ggctgtttgg
    12661 attacaggaa aaggaaatag taaaatagct tcatttgcta aaggtgctat atttatacca
    12721 gtattatcat ctatggtttt gataggtatg gtatggaggg ctttattgaa tggagaaggt
    12781 tctattatat atcaggtatt aggtgccttt ggaatagaat catctaaact tttaggagat
    12841 agtaagacag cattacctac acttatgttt atatctatgt ggaaaaatat agggtatttc
    12901 atggtaattt atatttcagc tataatgaat ttaccaaaac actgctatga agtagcgaag
    12961 gttgatgggg ctacaaaact tcaagaattt ataaaaataa ctgtaccttt attgaaacca
    13021 actacaataa tggttgtatt tttagggtcg atatggtcat tacaagtatt tgacttaatt
    13081 tatactgtga caggtggagg tccaggtata tctactatga gtatagttat gcatgcattt
    13141 aacttaaact ttaaaaactt taattctgga tatgcaatga ctgtagcaaa cgtattattt
    13201 ttgttaatag cagtagtttc gattttacaa aataagcttg taagacgaga caattcagat
    13261 ttttagaaag gtaggtataa tatgaaaaca gcaagaagat tattaagtga tggtttactt
    13321 ttatttatag cgtgtgcaag tttggtacct tttatataca tgttgataat atcattgaag
    13381 ataacatata actcatatag tttggatata tctttttcaa cagtaaccct tcaaaactat
    13441 atagatattt ttactaaaaa aggatttgca caatattttt ttaatacagc aatagtatct
    13501 ttttcaggtg tattattaaa tttagtattt agtacattag caggatattc ttttgcgaag
    13561 atggatttta aaggtagtga taagttattt ttatttatga taatgacttt aattatacct
    13621 tcccaagtta caatgatacc actatatata ataatgaaac acttaggatg gataaatagt
    13681 tatcttgcac ttattatgcc aataccaact gcatttggcg tatttataat gagacaagct
    13741 attttaggag taccaaaaga gttattagaa tcagcaaaga tagatggttg ctcggatttt
    13801 agaattttaa tacaaatagt gttgccttta attaaaccag cattaattac actagcaata
    13861 tttactttta tgggagcatg gaacgagttt atgtggcctc ttatagctac aactaaagat
    13921 gcaatgagaa cattaacagt tggattatct actcttaaga cattccagat aactaattat
    13981 ggacagatga tggcaggggc tacaataaca tttttacctc catttatttt ctatttgatt
    14041 ttacagagta aatttgttga aggtgtgtct ttgtctggta taaaaggtta aatagtctaa
    14101 tttaataata tataaagaaa cttatataaa tatggaggaa tttaaactat ggaaatttca
    14161 tatgtactga aagattgcac attcaaacac aataaatact cagttgtaga agctgaaact
    14221 tggaaagata aggatttgga tattgtagta gctaaaggtg aaaaatttgc atttcaaatt
    14281 atgttaaaag caacagaaga atttaattgt acaattgata aaagtagtgc tatatcatgg
    14341 aaaggcctgg gaaatagagt aaggttagca ttaaatatgc ctaatagttt agaaaataat
    14401 tttagtataa atatattagg atatgttcaa gatgatacaa aggcatttgt gaatgatgct
    14461 attttaaggg ataaagatgt gttagtagag caatatttgc ctcaaacatt ttggattgaa
    14521 ggtcaagtac cagaagattt tgaagaaaat aaccttgata taggtattga tatatttaaa
    14581 agctttggat atgaagacga agaaaaagtt tgtacaattg atgttcctgt aaaagtaaga
    14641 aatgttgtgc taaagccttt agatgaaagc aagttctttt tagatttatg gcaacatcca
    14701 agctgtttag ctagaatgta taaagttgag ttatggtctg atatacattt tgaaatagtc
    14761 gataactatt taaaagaatt agcatcttta ggagaaaaag ttgcaacagt tatagtttca
    14821 gattatccat gggcagggca aagttgttat aaagtttata aaaatccatc aaacttatat
    14881 gaatataaca tggtaagtgt atcaaaagga ttggatggaa agattaaatg taactttgag
    14941 agtatggata ggtatataag catagctgat aagtataaaa tggctaagga gatagattta
    15001 tttggtctat taggtaattg gtgtgctggt gagtttggaa atcctgtaga aggttataag
    15061 gaccctataa gagtaagata ttttgatgaa aaggataaag tatttaaatt tataaacaat
    15121 acaaatgatt taaaagaata tataggatta gtattaaatc atttaataga gtgtggatta
    15181 tgggacagag taaggattat agctgatgaa ccaaataatc cagaagtagt caaagaatgt
    15241 atagaattta taaattctac tgtaggtact catcaagtta aatataaatc agcaactcac
    15301 gaccaaaact ttttagacag agctaaagat gaaattgatg atatgtcaat aaatttaaaa
    15361 cttactatac aaaattataa agatatagaa agtcttaaga aaaaaatcaa tgacaagggt
    15421 gggatattga cttggtttgt atgctgtttt ccagagaaac caaatagttt tttaagttct
    15481 ccttttgtag aaaatagaat aataggatgg tatacttatt actttggtct tgatggattt
    15541 ttaagatggg actataattt atggacagaa gacccttgga aagattcaag ttacaaattc
    15601 cctatttgga aagctggaga tatgttcttt gtttatcctg gaaaagattt aaagccagtt
    15661 agaagtgtca gaatggagaa tttaagattt ggaatacagg actttgagtt atttacaatg
    15721 ttagaaaaag aaaagggaag agagtatata gaagttgagc ttatgcaaga attattaaat
    15781 aaaaaagaaa atgctgaaat taaaggtttt ggtgacatag aactaggata ttcacttgat
    15841 aattggggat atgataaagt taaaaaatat gtattagagt tgttagacag agtataaaaa
    15901 atataagagt gtctcaaaat gaacttttta gttcatgagg cactcttttt gtatgaaatc
    15961 aatatagttt cattgtttca acaatgaaaa ataggattat ttctattcta agacagccct
    16021 ttagtttttt agttgtattc aatgttttaa aactaactat aaacgctata aagtttataa
    16081 atgacaataa ttatatataa catgatgtac tttaatttgc tataataggg attgaataat
    16141 attatatgga agataacatt gtttgggggg attaatataa aaagaatagg tattattata
    16201 atgttttttt taagtatatc ttttattgga ttttatcttg taataagaga taacagtgat
    16261 acagttaaga tgcaacgagg gataaatata ggtaatgcac tagaatcacc taaggatttt
    16321 ccgtgggatg taaaaatgtc aaataagttt tttgatgata taaaagatgc aggttttgac
    16381 acagtacgta taccagtaag gttttctgat tatacgtcag attctgataa ttttaaaatt
    16441 gatgaagaat tttttaaaaa gattgataaa tatgtagatt atgcattgga taaagactta
    16501 atagttgtgt tggatttaca ccattttgaa gaaattatga aagaaccaag agttcataaa
    16561 gaaaagtttt taaaaatatg gcaacagata gctaataggt atcaaaaata tgataaaaaa
    16621 ttggtatttg aattattaaa tgaacctaaa gaaaatttat attcacagtt actaaacgaa
    16681 tacattgaag aagcaataaa gataattaga aaaactaacc ctaaaagaac tattatagta
    16741 ggaccatata atttctatca aattgattat ttgaatgaat taaatattcc taaagattct
    16801 aatatagtag tttcctttca ctattacgag ccaaatgatt ttgcatttca aggaaatata
    16861 tatcataagg gatttgagca cttaagcaat attacttggg aaggaacaaa cgaacaaatg
    16921 gattatttaa aaaaaaggtt tgatactgtg gaaaattggg caaataaaaa taaggtaaaa
    16981 atcttcttgg gtgaatttgg aataacaaaa gaggcaccag aagcttcaag aagagcatgg
    17041 ataaaagctg taagagaaga agctgaaaaa cgaaactttt catgggctta ttgggagctt
    17101 gcatctggat ttggtatata caatcaaatt gaagggactt gggatagaga tatattaagt
    17161 gcattaatag aaaagaggtg attttatgaa gaaaaattca aaaaagatac ttataacttt
    17221 aactattatt actaatgtca tatatatatt gtggagaatt ttttacacag tacctaaaga
    17281 agaagggaag tttgccttaa tatgtgccat aatacttctt tttgtagaga taatgggcat
    17341 gatggaaatg tttgtgcatt attatggtat gtcaaatatt gagtaccctg aaaaaccaat
    17401 cataagtgaa gaactttatc cacatgtaga tgtattcatt gcaacatata atgaatctgt
    17461 tgatttggtg agaaaaactg taaatgggtg tattcatatg cagtatccag ataagaaaaa
    17521 ggtacatata tacatatgtg atgatggaaa tcgtgaagaa atgtgtattc tagcagaaaa
    17581 aatgggtgta aattacataa ctagaactga aagagaagga gcaaaagctg gaaatttaaa
    17641 taatgcaatg caacatacaa attccccttt gattgctaca tttgatgcag atatgatacc
    17701 aatgcatgac tttttaattg ctacagtacc atactttttg aaaaatgagc aagctaaaaa
    17761 agatggagaa aaagaggagt atgaaaaggt tggttttgtg caaactccac agagttttta
    17821 taatccagac ttgtttcaat ttaatctgca ctctgaagga aggattccaa atgagcaaga
    17881 ttatttttac agagacattc aattggctag aaataggaca aattctgtaa tttatggtgg
    17941 tagtaataca gttatttcaa gggaagcact tgaagaagtt gatggatttt atacttattc
    18001 tattacagaa gattttgcaa caggtatctt aattcaaagt aaggggtata gatgttatgc
    18061 tattccagaa gtacatgcat caggtttatc tcctacagat ttaaaaagtc ttattaagca
    18121 acgtgaaaga tgggcacgtg gatgtataca aactggtaga cgtctaaata ttttgtttag
    18181 gagaggttta ggtttttggc agaaaataag ttatatttct tcaattactt attggtatgc
    18241 aagtataaaa cgctttgtat atataatggc acctatttta ttttctgtgt ttaatgtgat
    18301 tgtagtaaaa tgtacgttac ttcaagtatt ggtattttgg ttgccaatgt atattttaag
    18361 tagtttatct cttaaaatat tttctcaaaa tataagaaat actagatgga ccaatatata
    18421 tgaaactata atgtttcagt ctttaatgcc agcagtaata cttgaaacgt ttgcaatatc
    18481 taaaaataaa ttttcagtaa caaataagag taaattagaa gaaaatagaa tgtataagtt
    18541 tttacaggga ataccttatt ttatttacat ggtcttatct atcataggaa ttttaaaaat
    18601 gtttgtagca atatttaaaa tgagttcaat gacatattca gttgtattat tttggttgat
    18661 tggaaacctt tttaaccttg ttatggctac attatttata tcagggagac agcagttaag
    18721 aaagtctgag cgttatattg ctgaaattga ttttaagtta aagcaaaatt cttatgttct
    18781 ttcttcaaaa acaattgata tatcagaaaa tgggtttgca tttttgctag aaaatccaga
    18841 atatatttcc ccagaagaag agtttgaggt agagtttaga gaaaaatctg gaaatgaaat
    18901 gtatatagct aatatgaaag caaagattgt taatgttgtg gaagtaaatt caaagtggaa
    18961 atatgcagct tatattacac atattgaaga tagtgaaata gataattgga tgtgtattgt
    19021 tcacgataga atacctactt taccaatgac aatatctaat caactaggtt tctttgatga
    19081 tttgcaaatt aatgtaaaaa aacgtattga aaaaactaga acattatcac gaagaagtcc
    19141 aagaatcaat atgaattttc agatggatat aaaaaataca ggtaaattaa gaatagtgaa
    19201 ttttaattat caatatgtat tattaaattt tgagaataaa agtatttatc ctaaagaaat
    19261 agcattggaa attaatgaaa atattgtgtt agaatgtgat ttgtgtgagg gtaaaattga
    19321 tgaaagaggt atcttatata aagtaaataa cattgacagc ataatgcaaa atttcttttt
    19381 aagagatgaa atgatggatt ggatattaca aaataagaaa attcttgatt caaagcctag
    19441 tgaaaagaag gaaaaaagta tagatgagtt tgaacccatg gaatatattt aggagagatg
    19501 aaatgaagac tttaaaagtg tttttttgtt ctacaaaata tcttattttg gtgtttattt
    19561 tttttataaa tactactttt ataagttcag caacaagtga taaaatttta gatttatcct
    19621 ttaaaaagat agagactgac ttaagttcta agattacata tgaggataca ggtgtaaaaa
    19681 tagaaacgga tagttctaaa agcgataagg aacgttattt atatatttac caaaatataa
    19741 aagaaaattg gagcatgtat aataattttt atattgaaat tcaaaataaa aataaaagtt
    19801 ctcagagaat aaatttaagc atacaaagta aaaatatgct tgaatttaga ttaaaagaag
    19861 gtagtgaagt atttttagaa ggtaaaaata taatatattc agataaaata aaagaaggat
    19921 gcatagaagt accaggtgaa tttgaaggca aaatttatgt gaactttaat agccttataa
    19981 atgaggaaag taatgttgtg ctggactcaa atatgctttc aaacattgtt agttggggaa
    20041 ttacgtttat tccatctgat gaagaacata atattgttat tataaaaaag atatctttat
    20101 tgtctgaaga gaaattaaga tttttaaata atattaaaat aattggcgat gaagaagtac
    20161 aaataccagt attgggtcaa agtataagtc agtatgaagt attaggtctt aaaagtgatt
    20221 ctaaaattaa gtactctctt atgggaaaac aagataatgt aagcatatct caaaaaggta
    20281 aattaacatt aagtaataaa gccaaacctg gacaaattat tttacaagtt aatgttgatg
    20341 ataagtttaa aattggaaag aaaataacat taactgagtc ttggagtgtt aataaaaaag
    20401 ataaggatgg tgttccatat acattagtat atcctgagca atcaccaaca gtacaagata
    20461 tgaaaaaaat aaactttatg aataacatca taacgtttgt tcgaatttta tttgtatctc
    20521 tagtaataat atgctttgga atatatttat attggaaaaa atgttcaaaa actaagtaaa
    20581 gagaggaggt tagattgtgt tattttcaag tttaattttt ttattttatt ttttacctat
    20641 aactttggtt ttatattatg tatttaggtt taaccgaacg atacagaaca tgatattgct
    20701 tgctgttagt ttattttttt atgcttgggg agagccaaaa tttgttgtta tcatgatagt
    20761 ttccataata atgaactata tatttggttt actggtagat agatataggg aaagtaaaat
    20821 aaaagtaaaa gtttttttag ttttgatgtg tgcatacaat ataggtgttt tatttatatt
    20881 taaatatcta gcttttgcgc taagaaatat tagtgaactt attagtactg aattgactat
    20941 acctagtatt gttttaccca taggaatttc attttttaca tttcagggaa tgtcttatgt
    21001 tatagatgta tatcgtagac atggagaagt ccaaaagaac cctttttatg tgggattgta
    21061 tatagcattt ttcccacaat taatagcagg tcctatagtt agatatgaga gtgtggcaga
    21121 acaaattctc aatagaaaag aaacttggaa taaattttct attgggactt gtaggtttat
    21181 aacaggtcta ggtaaaaaag tattaatttc aaataacatg gctatagtag cagactatat
    21241 ttacactatg aatagtcaag gagaaatagc tgcatcactt gcatggcttg gttcaatagc
    21301 ctatactctg caaatattct ttgatttttc tgcttattca gatatggcta taggcttagg
    21361 tcttatgttt ggttttaaat ttgaagagaa ctttaattat ccatatatat caaaatcaat
    21421 tagtgagttt tggagaagat ggcacatttc tttgggaatg tggtttaaga gctatatata
    21481 ctttccgctt ggaggttcta gagtagcaaa taaagatatt atgattagaa atatgtttat
    21541 agtgtggtta tttactggaa tatggcatgg agcagaatgg acatttgtaa tttgggggat
    21601 tttaaacttt gttttcctaa ttatagagcg atttataatg tttgaaaaga ttgaaaatca
    21661 caattttata aaacatattt atgctttatt agtagttaat tttgggtggg tattatttag
    21721 gtcacctaat ttaaaagaag catataatta ttttaaagct atgtttggag gcaatggagc
    21781 tatatggagt gattatacat atatgttttt aaaagaatac tgggttttct ttatatttgc
    21841 atttattttt agtgtaccta ttgcaaagaa aatcaataag tttgttgttg aacaagctaa
    21901 atatagtatg atatttaacg tattttatcc aatctcagct attttattgt tttttatttc
    21961 tgtgacatat ttagtaactg gaagctataa tccatttatt tattttaatt tttagttggg
    22021 aggaaaagta tgaatagatt ttttaaagta agattaataa ctgcaattat gttcttaatt
    22081 attttattcg ctttctctat ctttaatgtt tgttattctg gaaaagatac gatacaggaa
    22141 ataaaaaatt caatagctga aaaaagtagt ttaaaagata caatatcatc tattgataat
    22201 acagtgaatg acaaagtaat atacaaaaat acgttcattg aaacttatgg ctatttacaa
    22261 aaattaatgg gtaaaaatga gttttcaaaa tttagtgttg taaaagatac ttctggaaag
    22321 atgcattata catattttgc taaagctcca aatccagttg gtgtgatagc agatagggta
    22381 agaaaatttt ctgatgaaat ggaaaagcaa aacactaaat tggtttacct aatgactcca
    22441 gataagtata taaaaggagt gacaaaattt ccaaaaggaa taccttattc ttatagtaat
    22501 gagacagcag ataatttttt aaatcaacta aaaaagtata atgtggacta tattgatttt
    22561 agagaaaata ttttaaagag tggtattcca aaagatgatt tattttttaa aacagaccat
    22621 cactggaaag tagaaacttc tttttgggca tttggagaat tagttgagca acttaataaa
    22681 aaatacaata tgaatttaga tgaaaatcat tattatagag ataaggacaa ttataattca
    22741 attgtatatc ctaagtcatt tcttggttct atgggaagaa agactggtat cttatatggt
    22801 ggtatagatg attttacatt aatttatcct aaatttaaga ctaattatac ttattataca
    22861 gattctaaat ctcaaaaatt tgaattaaaa ggaaggtttg aagagagttt aatattatca
    22921 tatcctttta atgcagacct tgatttaatg gatggtcaat cagataagta ttttacatat
    22981 ttattgggga acagaccatt agttaaaata aaaaatatag aaaatccaga tggattaaaa
    23041 gtgctgtttg ttaaagattc tctaattgta ccaacagcag catttttttc ctctgtttgt
    23101 tcatctatag atatgataga ccctagatat tatgatggag atatactaga atatgcaaaa
    23161 tcccatgact atgactttgt ttttatatct gtatatcccc aaaatcttac aaaagaattt
    23221 tttccgtttt gtgagtaaat ttctaaaaaa gttttctgat aaaataaatg gctaactggt
    23281 aaaaagttag ccatttattt tgagtgttat tctaagaaat atttttttta ctaaatactt
    23341 ctttatctat ttttccaagt gattttccta cagtaattga tgataccata gcaccattaa
    23401 catttatcat agtacgtccc atatctagta tagggtctat tgcaagtata ccaccaacta
    23461 atggaaaata agaaccaaga ccaactccag agataacaac tgaaacggcc attgtagcag
    23521 ttcctggaag accagctatt ccaaacgaac taattgttat aacaattaac aacataacat
    23581 aaaagctcat atccatgttt actccagcca tattggcaag tgttacagcc attaaagcag
    23641 gatatatacc tgcacaacca ttcataccca tatttgcacc taaacttgct gtaaagcttg
    23701 atacaccctc atcaactccg tgattttgta ctagactttc tatcgttaca ggcaatgttc
    23761 ctaaactaga acgtgaagta aatgccagaa gtaaagcttc agttgaattt tttatataag
    23821 taatgggatt tagaccattt aaagttataa aaattaaatg tataataaat aatattgcaa
    23881 ctgatatata aagagctaat ataaactgta aaacagatac tattgaacta ataccttgtg
    23941 aagttattgt atttgcaagc agagcaacca cagcataagg cataaattta ataacagtca
    24001 tagacacact tacaattatt ttgtaagatg cttctataaa acctataaat ggttttattg
    24061 tttcaaaatg ttttttactt aatctcttta ttgaggtagc tataaatgta gcaaatatta
    24121 ttgtagctac aatatttcca tttgccatag ccattacagg attagatggt agtaatcctc
    24181 ttaaagtatc cacaagagag tttatttctt taagttcttg attttcaact aaagtaggca
    24241 tattagctcc tacacctaac ttcattatat ttgcaacgat aattccaaca attgcagcta
    24301 tagcagtagt agcaagaagc atacctaaag atttaaatgt cagagttcca agattgttat
    24361 ctttcatatt tataattact tttataatgg ataagaaaac taaaggtact actaacattt
    24421 ttaataagtc cataaatcca gaaccaaata ggctatacca cttacttact tcttgtagcc
    24481 aaatgacttg agttgtatca ttaggaaaac cagcaaaagc ttgtattatg attcccaata
    24541 tgagtcctag tactgtagca tatattgttc tcaatgaaaa ttttattttt ttcttttcaa
    24601 attgtctaac aataaagaac gtacctagaa gtaatattat gaaaaaaatt gttttaaaat
    24661 ctgtaatcat aagaaattgt gataaaaatg aactttccaa tatttttctc cttttattta
    24721 aaaataaatt tttctaagtt taaatgatat caatatatta aaaaaatgtc aatattatat
    24781 cacaatttac ttaaatagag taaaacaagt tgattttgaa aaatgtgatt aagtacagaa
    24841 aaatagggta taataaatgt ttaaagaatt tgaattgtgg taagaaaata aaaaatagct
    24901 tttataagat ttgatttcaa attaaaatgt gttaaataaa taactaaaaa ttattataaa
    24961 aaatgttttc atagaatata tatcaaatga aaagcttaaa ataagtgaag aaattaacta
    25021 gacatcaaag gagagataaa atgagagtaa taataattgg tggtgttgca gctggtatga
    25081 gtgctgcagc aaaattaaaa agaataaaac ctgagtatga agttgttgtt tatgaaaaaa
    25141 ctgaaatagt atcttttggt gcttgtggat taccatactt tgttggtgga ttttttaatg
    25201 atgctgatga attattagca agaacaccag agaaacttag agaagctgga attgatttaa
    25261 atatatttag agaagtcgtt gaggttgata gtgaatctaa gaaaataaaa gtaaaaaaca
    25321 taaaaacaga tgaaatatat gaagattact atgataaatt gatgatagca actggagcaa
    25381 gaagtattat gcctcctata aaaaacataa aattaaagaa tgtttctaca ttaaaaagtt
    25441 tatatgatgg agaatattta aaaaaacttt taagtaatga agataataaa agagtgacaa
    25501 taataggagc tggttttata gggttagaag cagttgaggc atgtaagaaa ttaggtaaag
    25561 atgtagatgt aattcagtta gaagatagaa tattgccaca agtatttgat aaggaaatga
    25621 cggatgtatt agaagaagaa ataaatagac ataatgttaa tttgcactta gatgaaatgg
    25681 ttgtagaact ttgtggagaa gataaagtag aaaaagttat aacaaacaaa ggggaaatag
    25741 atacagatgt agtaataatt gctactggcg tgagacctaa taccgagttt ttgtcaaata
    25801 ctaatataaa aatgctaaaa aatggagcta tagtagttga tgagtatgga agaacttctg
    25861 tagaagatat atactctgct ggagattgtg caactataaa gaatatagta agtaatgaaa
    25921 atgtatatgt tcctttagct actggagcta ataaattggg taggatagta ggagaaaacc
    25981 tagcaggaag agaaatatct tatcaaggtt cactatcttc aagctgtata aaaattatgg
    26041 atatggaggc agcatcaact ggaattacag aaaggcaagc taaagattta ggcataaatg
    26101 tcaaatctaa atttatttct gattataatc aaactcatta ttatccagga agaaataaaa
    26161 tatatgtaaa gcttatttat gatgcagata caaaagttat tttaggcgga caagtggctg
    26221 ggtttaagga tgctgtccaa agagcaaatg tattggcagc tgctatcttt ggaaaaatga
    26281 ccacaaatca acttggaatg ttagatttat gttatgctcc accatttgca agaacatggg
    26341 atgttttaaa tgtagctgga aatgtatcta aataatattt agattatagc taatatagtt
    26401 gacaagcaat taagggaatg aatttaccta aactattttt atcatggtaa aattcattcc
    26461 taaattttta ttccaaattt ttattccaaa tttttagaaa taaggattcc attaaattat
    26521 tcatcaaaat ttaaacgcaa gcttaatcta tcttctccaa gaatagtatt ttcaaaaaca
    26581 ttagtagcat tttctaaata catactaggt atgtctaaag aaggactaaa atgagtcagt
    26641 aaaagttgtt tgacatttcc aagttttgca agattagcag cttctctaaa agtcatatgt
    26701 ttgtttctaa cagcttttga tatatctaaa tcatcaccat acatggcttc acatataaat
    26761 aaatcactat cttttatgaa atctggtatt gattctatag gtcttgtatc ggttgtaaga
    26821 cttatcttaa ctccctttct caattctcct aagaccatat ctggagtgta ttgttttgaa
    26881 tctaaaacta tattttgacc ttcttggagt ttgttccata atattttagg aacttcgttt
    26941 ttagttgcct tatctatatc aaatttaggg tttcttctaa aattaaaact gtatcctaaa
    27001 cactctatag agtgttctaa actgattgtt gaaatttcta aatttcttaa aactttatta
    27061 tctaaagaaa aattgccttg agggttttct ataattttta aagtgtatgg tacatattct
    27121 actaaatttc tcatagaacg tatacaatcg actattccaa ctggaccaac tatagttaaa
    27181 tcacttgtac gaccactatt cccaattgtt gataaaagtc ctaaaagtcc aaatatatgg
    27241 tcaccatgta aatgggttat acaaatcaag tctatatctt taaagccaca tttttttagc
    27301 ttcatagata cctgagtacc ttcaccacag tcaattagta tttttctacc tttatagttt
    27361 ataaatacag aggatagaaa tctgtttggc ataggtacat ttccaccaca tccaagtaaa
    27421 gttaaatcta tcattttttc ccctttctaa aattaacaca aatctgttgt ttgtttttat
    27481 tatgatatcc tattataatt attttaacat taaatataac aataaatgtg tatttgagtc
    27541 aagaaatatt gtcaaataaa gtttttatat agaaatgtat aaaaatttgg aaaacaaaaa
    27601 gtgaaaatta tatttaggag catttaaagg aggaaaattt aatgaaagta ttaaagaaaa
    27661 ttactatttt gtcattgatt acattaacct tagttggttg tagtaacgga gaagataaaa
    27721 aagtggttga aggttctaag aaaattcaaa attctaaaaa agatgaacag cctataatag
    27781 tacataaaga tgcaaatata ttgaaagata ggtatgagcc accaaaagga tttaaaagag
    27841 ttgatgtaga aaaaggtagt tttgaatcat ttcttaggaa tactaagcta aaaaattatg
    27901 gagagaaagt aaagtattat gatggaagaa ctaaggaatc aaaaggtatt tatgatagtg
    27961 tatttgatgt tgatattggc aatagagatt tacatcaatg tgcagatgcg attatgctta
    28021 tgagagcaga atacttgtat cagaataaac tatatgatga aataagtttt gattttgttg
    28081 atggttttaa ggcgaagtat tcaaaatggg cacaaggata tagaatttca gtaaaagata
    28141 gtggttcaag ctggtataaa gcaactgaat attcaacatc ttatgagagt tttagaaagt
    28201 ttatggatat agtgtttgca tattcaggaa cgttatctct tgaaaaagaa ttggagcctg
    28261 taaaagttga ggatatgaaa ataggagatg tgtttatagt tggtggaagt ccaggacatg
    28321 caagtataat tatggatatg gcagaaaatg aaaagactgg tgaaaaggta tttatgatag
    28381 cccaaagcta tatgcctgca caacaaacgc aacttcttat taatcgtaaa aatccaaatt
    28441 tgagtccttg gtatagttta gattttgaag gtgatttgac aatacctcaa tggactttta
    28501 aaagagacca gttgaaaaga ttttaaaata ataattttat ataataaaag aaaaatatag
    28561 atgtgaaaaa atgtaattag attaaaacta attggatatg gacaaaatta aattgataga
    28621 taaatattaa aaattacact aattaatctt actatagttt aatatttatc taaaaagttt
    28681 tatgtaaaat ataaatttta tattaaagct aaaactaatt tgaggaggct aatttatatt
    28741 gaagattaga aggtatttaa ttttggtaat tataattcta attacaacaa gtcagagttt
    28801 tatttcttat gggctagagc gatttgaaga aataaccatt tttcacacta atgatataca
    28861 tggaagatat gctgaaggtg atgaccatat acaaatagga aatctagcca cattgaaaaa
    28921 agaaactcca aactcaattt tagtagatgc aggagattgt ttgcatggac ttccaatagt
    28981 aaatatggat agagggaaaa gtgcaattga attaattaaa tgtgctggat atgactatat
    29041 tactcctgga aatcatgact ttaattatgg aaaagataga ctgtttgagt tatctaaaag
    29101 tgctaattta ggagagaata ctttaaaatt tttatcatca aatatttttg aaaatggaaa
    29161 aaaagttttt gatagtaatg atataaaaga aatagatgga ataaaaatag gattttttgg
    29221 actctcaacc caggaaacaa agagcaaaac tggatttaaa aatgtggaag gattagagtt
    29281 taaaaatcct atagaaagtg caacagaaca agtaaatgaa ttaaaaggaa agggtgccaa
    29341 tgtaatagtt gcaatatgtc atattggtac aaatagttta agtaagccaa atagtataga
    29401 tatagcaaat gaggttcaag gaattgattt gattattgat ggacacagcc acactaagtt
    29461 tgaaaatggt aaacaagtgg gtgatacatt gattgtaagt gcaggacaat atttagaaaa
    29521 tattggcgag ataaaactaa ctttggatac taatgatgta gaaaatataa aaatagaaaa
    29581 taaatcagtt agattaattg gaaaagaaga agcattaaag tataagcttg actccgctgt
    29641 aagtaaaaag gttgatgaaa taaaagaaca acaagaaaaa atattagata aagttatagg
    29701 ggtaactaca aatactctag atggttcata tgaaaatgta aggacaaaag aaactaattt
    29761 gggtaattta ataagtgaca ttttactaga taaaactaag gctgatattt ctttatttaa
    29821 tggagggaat attcgagata cgatagaaaa aggcaatata acaagaagag atattgtaga
    29881 tgtatttcct tttaacaata ctatagttac caaagaactt acaggagcac agattaaaga
    29941 tgtattggag catggagtaa aactttaccc agaaaaaagt tcagcctttt tgcaagtggg
    30001 aggaatttca tattacttta atcctaaaca aaaagaaggc gaaaaaataa caaatattca
    30061 aaaagaagga aagccattag atttaaataa aaagtatgta gttgcaacga atgattatat
    30121 agcatcaggt ggagatgaat ttccttgttt ttcaaaagag ccagtactaa aggaatttgg
    30181 aaatttagag agtgcagtca ttgaatatat agagtacaaa acagaaatat caaaaaatgt
    30241 agatggaaga attggtatta aatctaaaga tgagttaata agcaagaatc atgagagtga
    30301 tttgatatta agtgaaaatg aaaaaaatat caatgataat gaagagaatc tatctaataa
    30361 tataaaaagc tctgatagtg ataaaaataa aagcttagat aaaacaagtg ctagagaaag
    30421 ttctaatcta ggaatatcta aaagagaagc taaagaaaaa tctcctaaaa caggagattt
    30481 aggtttttca aatagtataa ttatatttat agtttcaagt acattgatat gtttattgaa
    30541 ttttaatcaa aaagagttaa aggataaaaa gtcaaaataa aaattaatga gcaaaaacta
    30601 gtagatagta atttaatttt agataatagt aaacagcaaa ggaaattagt tatataaata
    30661 ctaatttcct ttgttttatg ttttaacatg attactttta aataaaattg tattttatga
    30721 aaaataatta cactaaatgg catagtatta tggggatttt tagtttttat tcaagtaaat
    30781 gaaatcatat taacaacttc tgatatattt acaaattgat aaaaaaatga ttttattagt
    30841 atgatataat ttagctagta tatatgatga tggaggatat agggatgaat aatatattac
    30901 ttttggaaga tgacaaatct ttaaatagag gaattagttt taaattaaaa aaagaaggat
    30961 acaatgtttt tagtgcattt ggtatagaag aagcaaaaaa tattttcgca aaagaagaga
    31021 tatgcttaat tatttccgat ataggtctac ctgatggtag tggttttgac ttttgtgagg
    31081 agattagaaa gaaaagtgat gtttacataa taatgttgac agctcttgat gaagaggtgg
    31141 atattgtcac aggatatgat ttaggtgcag atgattatat cacaaagcct tttagcttga
    31201 tggtattaat atcaaaagtg aatgcgctta tgaaaagagt aaatacagta aaaaattata
    31261 ctctattagt ttgtgatgat ttattctttt attatataga aaataaatta attataagaa
    31321 cagatgataa ggaagaagag ataattttaa gtaagacaga gacaaaacta ttaaaatatt
    31381 tgatggaaaa ttctatgcag acattgacta aagagcaact gttagaatct ttatgggata
    31441 gtagtggtaa ttttgtagat gataatacga ttgcagtaaa tataagaaga cttagacaaa
    31501 aggttgaaaa aaatccatct gaacctaaat atataaaaac agtaagaggg gttggatata
    31561 tgtggggaga aaggagtata aaaaaatgct aaaaaatatg aacctcaaag atggtttaag
    31621 tgtgaaagat gattatataa tgagaagagt tattgtatat tctgttttat ctgtactaat
    31681 agtaaccctt gtatttatac tttttgaata tagcagatta cataaattgt atgaacatca
    31741 aataaaaaat actcaaaata ttgctggaat attgattgaa aaatatccta atgatgaaat
    31801 agatatagta aattctattt ataattctga ttatagtaag gtgtctactg gagaaaaggc
    31861 atttaagaaa tttggctatg gattggaaaa taaaatgtca aatgataaaa attttagctt
    31921 atacttaaaa tcttttttta aggaatcatt ttttattttt ttaatgatgt ttatagtagt
    31981 tataggtata atatattatt ttataagata cataaataaa agattgagta aaatttattt
    32041 tatagtagat aagatgtcac aagaaaacta cttgaaggac actactgagt cagaagaaac
    32101 tggttatcta gtagaatata atgaatactt taaagaaggc atatttagta agataaataa
    32161 ctgtctatat gagcttaata gaagtttaaa gataaagttt attaaattag aaaaagaaaa
    32221 agaaagtgta aaatcattag taacagatat ttcccatcaa ttaaaaactc ctttagcatc
    32281 cttaaaatta tacaatacat tacttataga agaagaatta gaagaagatg aagccaacga
    32341 gttcttattg accaataagg cttctataaa taaattagaa aatttaataa attctttagt
    32401 gaacatatca aggttagaaa ttagcatgat aaacataaaa aaagaagata atgatataaa
    32461 aaatactatt ctaaatgcag taaaaagtgt tacccctaag gctaagagca aaagtataac
    32521 attaaattta aatgaattta acagtagaat tataccacat gataaaaagt ggactgagga
    32581 gagtattttt aatgtattag acaatgctgt aaagtataca caggtaaatg gagaaataaa
    32641 tatatctgta gaagaaactg ctaatttctt taaaattata atagaggata atggcatagg
    32701 gattgaaaaa aatgagttta ataatatatt taaacgtttt tatagaggtt ctgctgatga
    32761 aattgaatct gtagaaggta gtggggtagg attgtattta agtagaaaga ttttagaaga
    32821 acaaggtgga aatattatag tttcttcaaa agtaggaata gggagcaagt tttctttgtt
    32881 tcttacaact atgtaatatt aaatgaaagg aaaatgtaag cctctacatt tattatataa
    32941 gtataaacag ctccagaaat aaaaaattaa taaaatatgg ggtaatataa cttagaatga
    33001 gaggataatt ttatgaaaat actatatact gaaaatttat caaagcatta tggaaaagga
    33061 gaaagcttag taagagcttt ggacaatgtt gatttagaaa taaatgaagg tgaatttgtt
    33121 gcaataatag gaaaatcagg ttctggaaaa agtacacttt tgcatatgat tggaggtctt
    33181 gatataccta catctgggaa ggtgtatata gataataaaa atatatttac attaaaagaa
    33241 gaagaacttg ctgtatttag aagaagaaaa atagggttta tatttcaaag ctataattta
    33301 attccatctc ttaatgtatg ggaaaatgta gtgttgccaa tcggtttaga tggtagagag
    33361 gtagatgaat cttttataaa agaacttttg aaatcattag gacttgaaaa taaacacgac
    33421 gttttaccaa atacattatc aggtggtcaa caacaaaggg tggcaatagc aagagcatta
    33481 gctacaagac cagctataat acttgctgat gaaccaactg gaaacctaga ttctaaaaca
    33541 tctgatgagg ttatgagtat attaaaatct atgtctaaga agtacagtca aactctagtt
    33601 atgataactc atgatgacag tatagcccaa atggctgata gagttatatt catagaagat
    33661 ggcagggtgt caaaggttgg tgataaaaat gattaatact actaaaatag caaaaagtaa
    33721 tttaaagcaa aataaatcaa aaagtattct aataataatt actataatac tttcaacctt
    33781 aatgctttct tctattggaa tttatattgt tgatgcagga gcatatcaaa aagaaaatac
    33841 tataaagtat tcaggcaatt atcaaggtat attagcaaat gtagatgaaa aacaggctga
    33901 tatattgagt aatcatgcag atgttgaatt aactggtgaa atgaatggag taggtgttga
    33961 aaaactggaa gatgattcaa atatttctct agcatacatg aatgaagatg ctttgaaatt
    34021 aaatagtttt gaatttatca aaggaaaatt gccatcaaaa gagaatgaaa ttgtgttaga
    34081 tagtggagct ttaaaggcac ttggttataa aaataaagat ttaggtgaaa agataaaaat
    34141 atcttatgat gactataaaa gtgataaaaa aattgaaaaa gagttcatta taagtggaat
    34201 cttaaaaact agcgaaatat ctgaagcagg taaatattat tatgctatta tatcagaatc
    34261 atatatgaga aatactagag atatgtcgca agaagatttt aatatatatg tgaaattaaa
    34321 tgataaaagt aatttatcga tagaacaagc aaaatcaaaa ttaaataaga ttgctaatga
    34381 tgtaggattg gacactatta atgtagctgt aaatgaaaac tatataaatg ctttaaaacc
    34441 agatatggaa acaattatgg gtggagtatt tgtagggtta gtaattgtat tgtctagtat
    34501 tttagtaata tacaatatat tttatatttc aatagttaca aaggttcaag aatttggaaa
    34561 gcttagagca attggtgcaa ctaaaaaaca agtaaaaaat atagtattta aggaagggtt
    34621 catattagcc ggaatagcaa taccaatagg aataatttta ggctatgtac ttgcaaatat
    34681 tataataaaa tcatttatgg atatagatgt aaaatcatca cgattaccag ttatattatc
    34741 ggttgtggta ataagcttta tatctgttgt tttatcactt ttaaaaccaa tgaaagtagc
    34801 atcaaaagta tccattgttg acgcagtaag atatacagga aataaaataa gtaataagag
    34861 taagagaaaa ggatataaaa atataaatct aaagagatta tcacatgcca atttagaaag
    34921 aaataaaaaa cgtacttaca tgacattggc atcacttata cttagtggaa ctatatttat
    34981 aacagtatca actgccttag aaagttttga tgctgagaaa atggcaagag aacactttcc
    35041 ttatgatata gaagttagac tatctggtta tgaaatgaac agtgataaaa atcctaaaga
    35101 taatttgaat attttacaaa tggataatcc tctaagtaaa gattttttta atcaaataaa
    35161 gaacatggaa ggaattaaaa gaatagaatc tgcaagaagt gtaaaaattg gtatggagga
    35221 ctatgatgtt gaatttaagt atgacctttt acaaagtata aatgaaaatg atgtaaaatc
    35281 attaaacaag aatataatag atggaaaaat aaatcttgaa agacttcaaa ctggagatga
    35341 aatagtaatt actcatgttg atacagcaaa agaaatggga gtaaaagcag gagataaaat
    35401 aagattaact ttatatgatg gagataaaaa gataaaaaaa gaatttaaag ttcaagcaat
    35461 agctatgggg gttccaagtt ttggtatagg gaaggatttt attgatagaa ctttgaaata
    35521 cgattcaact agtagcttgg gaatatatac agaagaagga aagtatcaag aaatcaaaga
    35581 tagtatcaag aagattgcta aaaataatgg atttttagag actgatttta tagatagtag
    35641 aattgagtca aataaggcta ctatttcatt tataaagata atgggataca cgctaacagg
    35701 cataataggt gtgattggat ttatgaatct tgtcaacaca atgataacta gcatagttac
    35761 tagaaaaaaa gaacttggta tgcttcaagc tattggtctt actaataaac aattagtgaa
    35821 aatgcttaat tcagaggcaa tgtattacac ttctggaatg atgataggaa gtattctatt
    35881 tggaggtata ttagggtata ttgctgttat atttctaaaa aagactggat tatcttatgc
    35941 aacatattct ttgccaatag taccaatctt gttgatgatt gtgtgtattc ttatagctca
    36001 atttataaca acttatttga taggacgaag ttttaataaa gaatctctta ttgatagagt
    36061 aagatacagt gaataaaata caaatattta tggttagttt aactagcaaa aaacatataa
    36121 aaaaatatag ttaaagagat tcataattaa ataggttaaa gcatagtaca gagcaaacaa
    36181 tttaagcaga tgtttgctct gtattttatt atgtaatcaa taatatatct aatatactga
    36241 tattattcat gttagaaaat gtaccggtac aattctaaaa aagtataaaa ttgtaccata
    36301 taaaatgaaa attttgtata ataatcgtat agaaaataaa taggagctga tatatatgaa
    36361 taagaagatt aattatgcac caggaccaac tgaaactagg gaaaatgtaa gattagcaag
    36421 agcagaaaaa acaacaaatc cagatataga tattgatttt gttgaatttt ataaaaagac
    36481 atgcgagaag tttggtaata taattggaac aaaaaacgat gtttacatat tgagtggtga
    36541 aggaatatta ggtcttgaag cagcatgtgc atcacttaca gaaaaaggag atagagtact
    36601 tgtaattgac aatggtatat atggagaagg ttttaaagat tttgtaacaa tgtatggtgg
    36661 ggagtatgtg cttttttcaa gtgaatatac taaaagtata gatattgatg aattgaaagc
    36721 atttttagat gaggatagta attttaaata tgcaacagta gtgcattgtg atactccaac
    36781 tggcgtatta aatgatgtaa gcaaaatatg tccattactt aaagaatatg gaatattaac
    36841 tgtagttgat tctgtagcag gtatggtagg agaaagatta agtgtagatg aatctaaaat
    36901 agatataata ttaggtggtt cacaaaaagc tatttctgca ccagcaggac ttactatagt
    36961 cggaataagt caagatgcaa aaaattgtat aaaaaataga aaaactgatg taataggttt
    37021 ttattgtaat ttaaatatat gggaaggcta ttatgaaaaa aaatacttcc cttatacaat
    37081 gccaataagt gacataatgg ggcttgataa agcacttgat aatatattag aagaaggaat
    37141 agaaaatgta ttaaatagac atgaaaagat agcttatagt gttagaaaat ctattgagga
    37201 atatggtcta gaactatttt tagaagaagg atattctaat acagtcacag ctataaaaat
    37261 tcctgaaagt ataggagctt tagaacttac tgattatatg cttaaaaatt ataatacact
    37321 tgttgcaaca tcactaaatc aatatatgga tactatttta agaataggtc atatgggaga
    37381 gaatgcaaat ctaaataaaa tagaacatgt actaaatgtt ttggataaaa gtttaagtgc
    37441 attaggattc aaaggaaatg gaaatttgtt aaatttattt aataagtatt atttttaata
    37501 gataggggga tatagtatgg aagttacagc taagcaaaaa tttacaacga aagacatggt
    37561 ggaaacctca ctattaatag cattagtatt tattgcaact aaatttataa acataagatt
    37621 accaatatct ataaatggtg ggcttgtgca tttaggaact gcaatgttgt tcatatcagc
    37681 aatagtattt ggaagtaaaa aaggtgcttt atcaggagct attggtatgt cactttttga
    37741 tttgatatct gggtggactt tatgggcacc gtttaccttt atagatagag gagttatggg
    37801 ttatttactt ggaaagattg catgggcaaa tggtaataat gggaataatt ttttaatcaa
    37861 tgtaatagga atatgtgttt catcaatttg gatgctattt ggatattatg taacagaagt
    37921 tatactttat ggtaatttta tagtacctct tacttcaata ccaggtaatt taatgcaagt
    37981 attgataggt cttataatag cgcttcctat ttctaaggta ttgaaaaaat gtataaaata
    38041 aatataaagt gaactaaaat ttatgatagc ataaattata ctgtataata ttaggatgga
    38101 gtacataatt aaaaaatttt agtatagagg ttagttcaca atgttatcag ttacaagata
    38161 ttcaatcaaa aatgttcgtt taaagccgtg ggtaaagttt atttggtatt ttgagacaaa
    38221 atcaaatata ctgttaaata ataagttact tccgacagac agtatagata ttattctcaa
    38281 tctttctgat gttatggaat ataaaattga aaatcaagac tatattgcta gtaatattca
    38341 ttttaatgga ataagggata aacatggatt tattattcaa catggaaata tacgtgttat
    38401 agggatttct ttttatccat ttggtctcta tccattttta aagataccaa tttcagagtt
    38461 taacagacaa attgttgacc tagaagcagt atcaaaacta tttgcaaaaa aattagaaga
    38521 gtcattaaat ccaacaaaag caatagaaaa agttgtttta tgtttagaag aagtattatt
    38581 atccatatta gaagaagatt taatttcaaa taaatatgta aagcttctaa attcatttat
    38641 atatgttaat agatatagca atataaaagc attttgtgat gatacaaata taaatattaa
    38701 aacattggaa agaatctgtt taaaatatac agggtataca cctaagattt taaagagaat
    38761 ttacagattt aaaatggcaa gcaatcaact tatttataat tataaagatg atgaactttt
    38821 tgattttatt tatgaaaatg agtactatga ccaagcacac tttataaaag aatttaaaaa
    38881 attttcaggg acgtcaccaa ttaaatttag tggtgagaat aagacgatta aagaaaatat
    38941 aaagtacagc tatttataat tatactgtcg attttttaca atacctccaa tttatttttt
    39001 gatatgatat aggaaaataa attggaggtt ttacatatga aaattattac agaaatagtt
    39061 gagtttaatg tggaagaaaa tttatcaaaa gaagagttta tagaaattgt aaatgactta
    39121 gaattaaaat ttcattctat tcaatctggt tttattgata ctgaactttt atatgatgaa
    39181 aagtctcaaa agtggattat gatacaacat tgggaatcta tagaagacct caagagtgtt
    39241 tctagtaaaa tgtttaaaga ttcttcaact gagaaattta gaaaagcttt aaagaatcaa
    39301 actgttaaga tgaatataat accacaaatt aaaagttgga aattatagaa ctgaatgtaa
    39361 aagatagtta aatagtatca aactaaatta gccaaataac attttgtatt taaattgtta
    39421 tttggctttt agtgtttatt ttaatatgag tgttatatac tagaaaaacc aacaaactgt
    39481 tagagtgtgg attatcataa tgtcatattc gggagctaaa aggcttaata ctgatttgcc
    39541 aattggtcaa tccatgcatg tccatctgga tatgctgtta ttaaagcatt tacaagccaa
    39601 tccctttgct cttgtgataa tagtgggact gttacatcga agtctttttg ataaccaagc
    39661 ctatctatgt atattgattt gcaaaacaaa accacttcgg gagccatata tgggatatta
    39721 tctgatgtta tcagtctggc cttatccata tctcttataa ttctttgatt ttgggaaaaa
    39781 agaaattttc catctttctg tggattatat atcaattcaa taaaatcgaa ctgtaactgt
    39841 tcgtcacact gtatattcat tttatagtta tcatcctcga ctttttttaa ttcaataaat
    39901 gagcaatcag gcttaatcgc aaaaacacaa atttgctcta tatgttgctt gttaaggtcg
    39961 ataataggtc gtaataaata attacccaag ggctcatata tatcccatcc ttgattaagc
    40021 atatactgaa tgctcttttc tttatctgtg tcagaaatcc atatgtcaat atcactgtgt
    40081 gggcgaatat gcatattcgc aaataattct agggcatatc caccacatat tgaatatgaa
    40141 aagccacaat cattaaaaaa gtttacacaa tctttaatta gtttgttcat agcaacctcc
    40201 ttttgcatgt actgaaataa attaaatttt ttaaaatacc aactaaatgt ttgttttgtg
    40261 taaatataaa actgccgata ttaaggcagt tttatattta ctatttagaa aatttattaa
    40321 caaatttttg atattcacac attatcttat cattcataaa agaaataccc ataaaagcag
    40381 cttgctcacc aaacaaagta aaacgttcta tcatctgttg tggattacct ggataaactg
    40441 ttggaataca ccatgtgttt agaagaaaac caaacatttg agcagcaatc tctggatttt
    40501 taacttccat agaattatct tgatttcctt cttcaatcaa tgcttttatc caaggcacaa
    40561 tatcatttat aatctcatca aactgcgtag ctaaatactt aggattacgc aatagtgatg
    40621 atgacattaa aaaaatgtct tgtttattgt gctgttcata agcatgaagc aacctatatt
    40681 gtaatttttc taatgcggtc tttccttcat cattttttac ctcataccca taaaatccat
    40741 cattatacaa ccgacttgtg attgccttaa ttatttcgtc ttttgattta aagtgatgat
    40801 aaatagcacc tcttgaaagg ccatctaaat tatctacaat gtcttgaatc gttgtctgct
    40861 catacccttt ttctaagaat aacttatgtg aaatatcaag tattttttgt atagtattat
    40921 caggttcttt ttttcttccc aaaaaactca ctcctcgaaa caatcatttg gtatgtttat
    40981 cttagcaaat acataatgat ttgtcaatgt gtctttttag catgttgaac aggtatattg
    41041 ccatgctttc attattttat ttttttgcct tatactggac tatatttgca gtttcctaat
    41101 atatttggct catatttaaa taccattatc taaaagcact attgctgtac gagaggcaat
    41161 aggcatttta gaagaaggag gagttgagct aaagtataca tcataatctc ttaagttttc
    41221 tttttttgtg tttcctccaa actctaccat atcagtattt aatatcgttt tgtagtttgt
    41281 attttgaaac tttgaaatat caactgattg tattagatta gggtggaaat taaatataaa
    41341 taaatatttg cccttactgt acaccaaaat ctttttatca ttatccatat gtataagttc
    41401 atgactattt tggataaaaa tatcactgta ttttgtaagt tcgagcatag atttatcaaa
    41461 atttaataat tgtttatact ttaagttgtt atcttctgaa aggctccatt gtcttctggc
    41521 atacttatag ctccaattgt tgccttctcg tggaaaatca atccactcag gatgtccaaa
    41581 ttcatttccc ataaaattta gatatccttc acctgctaga gaaaaagtta ttaatttaat
    41641 taatttaata agagatattg ctctattgat aacatggtta tttgagttta tttccatatt
    41701 ccagtacatt tccttgtcag ctagccaaaa aataattgtt ttatcgccaa ctaatgcttg
    41761 gtcatgggat tcacaatatc ctatattttt ttctccaggt cgtctagtag taagttcata
    41821 ccacattttg cccaaatccc agtcttcatc agataagtta gagatagttt ttatccaaaa
    41881 atcaggtact cccattgcga gtctatagtc aaatccaatt ccgccgtctt ttatagggat
    41941 acacatacca ggcattccac tcatatcttc tgcaatactt atcgaattag gtttaatttc
    42001 tttcattaat tcatttgcaa attggagata tgttatagct tcaatatctg tattcatact
    42061 aaaatatttt tcataactat caaatgaaac tccaagacca tggttatgat ataacattga
    42121 agttacacca tcaaacctaa agccatcaaa atggtactca tttaaccaaa attttatatt
    42181 tgaaagcaaa aaatgtatta cttcaggttt accatagtta aaaagttttg taccccaagc
    42241 aggatgattt ccctttgaac ctgagtggaa aaattgatgt tcacttccat caaactcatt
    42301 tattccttct aaagtattct ttacagcatg agaatgaact aaatctaata aaacagcaat
    42361 ccccattgaa tgagcagtgt ttatgaggtt ttttaagtct tcaggagtac caaacctaga
    42421 tgagattgca taaaagtttg aaacttggta tccaaaagat gcataatatg gatgttccat
    42481 aatagccata agctgaatgg tattgtatcc agcttttttt atcttaggaa gtattttttc
    42541 agtaaattca ttataagttc caatagattc gctttcagta gacataccta tatgacattc
    42601 atagattagt ggagaagtta tgttttttaa gtcaaaatca ttgtcagtcc agataaatgg
    42661 agtttttggt tgccatattt gtccattaaa tccaccaaaa tctttttgaa ttactctttt
    42721 tatatataat ggtatacggt caaatgtttt tccatttgca gtaacttgta cctttacctc
    42781 tgatttgtga ggtaagctat tttgaccagg aataaatatt tcccaattac cagacccaat
    42841 ttttcttaga ggatgagatt ttctattcca attattaaag tcaccaatta gtgacaaact
    42901 atctgcattt ggagcccatt ccctatagaa gaatccatca tttgtctgat gaaatccata
    42961 gtaaagatga gcatttgcaa aagaactaaa atctttatag tttttcaatg tttttgattt
    43021 catatcctca taatgtttca ttcgcttttt tatatcagat tcaaatggtt gtaggtatac
    43081 atcaatatct aaaattttaa atcttttttg acttttcatt gtatttctcc tccataataa
    43141 atattacatc taaaaaataa tatttatctc atactatctt ctctgtataa ttgatatata
    43201 ttatataatc ctaataaaaa gcttaagcca ccaatgatta atttcataat ttacctccct
    43261 agtatttata cttatatatc gtattcatca aaatttaaaa gtttgtttaa aactctttta
    43321 ataatatcat aatcaaagcc tttataggaa agatgttgag aaattttttg atatattttc
    43381 tttttatctt ctttctttac tctttcatac cttttttttg ctagatacat agcattttca
    43441 aattcagtgt ccttgtctaa ttcagatact gcttcattga ttgtacttct attgatacct
    43501 ttattatata aattctgctt aattctgttt ttgccacatt tatttaggtt aacatttgtg
    43561 ttaacaattt tttgagctaa tacactgtca tttacgagat tataactttt aagaaaatct
    43621 atgaccctct caattatatt ttcatcaaat tctgatgata gtttttcttt taattttttt
    43681 tcagattggt cagcatgaga taaaatattt aatgctttat ttttagcttt tatatacatt
    43741 tcatcatcta aaataggttt taattcttct tcatttattt cattcccttt ttttaaatta
    43801 aatgtataaa ccagttcttt aaatatagca ataaaaaatt tatcatctac ataaatatta
    43861 actctatcat catttctttt ttgttgttct atttt
//
LOCUS       DAELNY010000013        43644 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.013, whole genome shotgun sequence.
ACCESSION   DAELNY010000013 DAELNY010000000
VERSION     DAELNY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.013"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            180..2324
                     /gene="fdhF"
                     /locus_tag="KPE55_000604"
     CDS             180..2324
                     /gene="fdhF"
                     /locus_tag="KPE55_000604"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:594..596,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG0491705.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            2373..3206
                     /gene="fdhD"
                     /locus_tag="KPE55_000605"
     CDS             2373..3206
                     /gene="fdhD"
                     /locus_tag="KPE55_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG0491706.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            3217..3390
                     /locus_tag="KPE55_000606"
     CDS             3217..3390
                     /locus_tag="KPE55_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491707.1"
                     /translation="MCTKKEKQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            3448..3999
                     /locus_tag="KPE55_000607"
     CDS             3448..3999
                     /locus_tag="KPE55_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0491708.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            4024..5400
                     /locus_tag="KPE55_000608"
     CDS             4024..5400
                     /locus_tag="KPE55_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0491709.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            5400..5915
                     /locus_tag="KPE55_000609"
     CDS             5400..5915
                     /locus_tag="KPE55_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0491710.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(5993..7276)
                     /locus_tag="KPE55_000610"
     CDS             complement(5993..7276)
                     /locus_tag="KPE55_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG0491711.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKNLNKERLDKLMQ"
     gene            7465..7887
                     /locus_tag="KPE55_000611"
     CDS             7465..7887
                     /locus_tag="KPE55_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG0491712.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            7989..8927
                     /locus_tag="KPE55_000612"
     CDS             7989..8927
                     /locus_tag="KPE55_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG0491713.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            9113..11137
                     /gene="ligA"
                     /locus_tag="KPE55_000613"
     CDS             9113..11137
                     /gene="ligA"
                     /locus_tag="KPE55_000613"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG0491714.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            11297..12646
                     /gene="rph"
                     /locus_tag="KPE55_000614"
     CDS             11297..12646
                     /gene="rph"
                     /locus_tag="KPE55_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG0491715.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            12646..13116
                     /locus_tag="KPE55_000615"
     CDS             12646..13116
                     /locus_tag="KPE55_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0491716.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            13310..14596
                     /locus_tag="KPE55_000616"
     CDS             13310..14596
                     /locus_tag="KPE55_000616"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG0491717.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            14746..15330
                     /gene="clpP"
                     /locus_tag="KPE55_000617"
     CDS             14746..15330
                     /gene="clpP"
                     /locus_tag="KPE55_000617"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0491718.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            15349..16599
                     /gene="clpX"
                     /locus_tag="KPE55_000618"
     CDS             15349..16599
                     /gene="clpX"
                     /locus_tag="KPE55_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG0491719.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(16670..17197)
                     /locus_tag="KPE55_000619"
     CDS             complement(16670..17197)
                     /locus_tag="KPE55_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0491720.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(17185..17697)
                     /locus_tag="KPE55_000620"
     CDS             complement(17185..17697)
                     /locus_tag="KPE55_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0491721.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            17877..20240
                     /gene="lon"
                     /locus_tag="KPE55_000621"
     CDS             17877..20240
                     /gene="lon"
                     /locus_tag="KPE55_000621"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG0491722.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            20230..20841
                     /locus_tag="KPE55_000622"
     CDS             20230..20841
                     /locus_tag="KPE55_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG0491723.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(21106..22476)
                     /locus_tag="KPE55_000623"
     CDS             complement(21106..22476)
                     /locus_tag="KPE55_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0491724.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(22651..22766)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23054..24133
                     /locus_tag="KPE55_000624"
     CDS             23054..24133
                     /locus_tag="KPE55_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491725.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            24525..25205
                     /locus_tag="KPE55_000625"
     CDS             24525..25205
                     /locus_tag="KPE55_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0491726.1"
                     /translation="MKKQYVFIILTSTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            25198..26577
                     /locus_tag="KPE55_000626"
     CDS             25198..26577
                     /locus_tag="KPE55_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0491727.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            26748..27833
                     /locus_tag="KPE55_000627"
     CDS             26748..27833
                     /locus_tag="KPE55_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0491728.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANIGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            27887..28246
                     /locus_tag="KPE55_000628"
     CDS             27887..28246
                     /locus_tag="KPE55_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG0491729.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            28299..29171
                     /locus_tag="KPE55_000629"
     CDS             28299..29171
                     /locus_tag="KPE55_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG0491730.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            29172..29615
                     /locus_tag="KPE55_000630"
     CDS             29172..29615
                     /locus_tag="KPE55_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491731.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            29630..30214
                     /locus_tag="KPE55_000631"
     CDS             29630..30214
                     /locus_tag="KPE55_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491732.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            30467..31504
                     /locus_tag="KPE55_000632"
     CDS             30467..31504
                     /locus_tag="KPE55_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0491733.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGTNLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            31585..31950
                     /locus_tag="KPE55_000633"
     CDS             31585..31950
                     /locus_tag="KPE55_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491734.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            31955..32272
                     /locus_tag="KPE55_000634"
     CDS             31955..32272
                     /locus_tag="KPE55_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491735.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            32320..33924
                     /locus_tag="KPE55_000635"
     CDS             32320..33924
                     /locus_tag="KPE55_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491736.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNSLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            34048..35676
                     /locus_tag="KPE55_000636"
     CDS             34048..35676
                     /locus_tag="KPE55_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0491737.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            35941..37290
                     /locus_tag="KPE55_000637"
     CDS             35941..37290
                     /locus_tag="KPE55_000637"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG0491738.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            37446..38525
                     /locus_tag="KPE55_000638"
     CDS             37446..38525
                     /locus_tag="KPE55_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0491739.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            38870..39778
                     /locus_tag="KPE55_000639"
     CDS             38870..39778
                     /locus_tag="KPE55_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0491740.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            39783..42152
                     /locus_tag="KPE55_000640"
     CDS             39783..42152
                     /locus_tag="KPE55_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG0491741.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            42477..43280
                     /gene="proC"
                     /locus_tag="KPE55_000641"
     CDS             42477..43280
                     /gene="proC"
                     /locus_tag="KPE55_000641"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0491742.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSELVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        17956 a         4665 c         7927 g        13096 t
ORIGIN      
        1 gttgaaatat aaagattatt atgacatatt ttttttgaaa tttaagagga attgtgagaa
       61 aattgttgaa tttaatagat agtattgcaa aatatttatg attttgcata ttataaaaac
      121 actcattatc tttaagataa attttactta tcaggggata aatctaaggg gggaattgta
      181 tggagaaaaa aattttaaca gtttgtccat actgtggagc tggatgccaa ttatatctag
      241 tagtaaaaga taataagata ttaagagctg aaccggctaa tggtagaaca aatcaagaaa
      301 gtttgtgttt aaaaggtcgt tatggatggg attttctaaa tgaccctaaa attctaacac
      361 ctaggctaaa aaagccaatg ataagaaaac aaggtgtact agaagaggtt gaatgggatg
      421 aggctataaa ctatacagct tcaagactaa atgaaattaa agaaaaatat ggacctgatt
      481 ctattatggg aacaggttct gcaagaggac ctggtaatga agcaaactat ataatgcaaa
      541 aatttatgag agctacaata ggaactaata atgttgacca ttgtgctcgt gtctgacatg
      601 caccatctgt agccggtctg gcttactcat taggtagtgg tgcaatgtct aattctatac
      661 cagagataga aaatgcagat gttttattta tatttggata taatggagca gattcacatc
      721 caatagtggc aaatagaata gtaaaagcta aaaaaaatgg tgctaaatta attgttactg
      781 accctagagt aactgaatct gcaagaattg ctgatataca tctacctata aaaggtggaa
      841 caaacatggt tttagtaaat gcttttggaa atgtattaat agaagaaggt ctttacaata
      901 aagaatttgt tcaaaatcat acacaaggat ttgatgagta taaagaaatt gttaaaccat
      961 atacagctaa atatgctgaa aagattacag gaatcccaga agagcttata agaaaagcta
     1021 tgagagaata tgcaaaaggt aaaaaagcta tgatacttta tggaatgggt gtctgtcaat
     1081 ttggtcaagc tgttgatgta gttaagggac ttgcatcaat tgctctactt acaggtaatt
     1141 ttggtagaga aagcgttgga ataggacctg tacgtggaca aaataatgtt caaggagctt
     1201 gtgatatggg agctcttcct aatgtatatc ctggctatca aaatgtaact gatgataaga
     1261 taagagaaaa gtttgaaaaa gcttgggggg taaaattatc tcctaataat ggttatagtt
     1321 taactcaagt tccagactta gtactaaaag agaaaaagct taaagcttat tatatatttg
     1381 gagaagaccc agtgcaaagt gacccagatg cgtcagaagt tagagaagca ttagatgaat
     1441 tggaatttgt cattgttcaa gatatattta tgaataagac agcacttcat gctgatgtta
     1501 tacttccagc tacttcttgg ggagaacatg agggagtata tacatgtgca gatagaggat
     1561 tccaattgat gagaaaagct atagagccac aaggagatgt aaaaccagac tggcaaatta
     1621 taagcgaaat ttctacagca atgggatatc ctatgaatta taaaaatact aaggaaatat
     1681 gggacgaact tagacaatta tgtccaagtt tcttaggtgc tacttatgaa aagatagaaa
     1741 ctcaaggatg cgttcaatgg ccttgtaaga gtgaaagtat ggaagataaa ggaactatgt
     1801 atctgtatga aggtcaaaag ttttctactc ctaatggtaa aggtaatttg ttcgctgctg
     1861 aatggagacc ccctatggaa gttgaagatg atgaataccc atttagttta tgtactgtaa
     1921 gagaagttgg acactattca gtaagaacaa tgactggtaa ttgtagaaca cttagttcat
     1981 tagaagatga accaggacgt gttcaaataa attctaatga tgctgaaaaa ttaggaattg
     2041 aagatgatga attagttaga attagttcaa gaagaggaag tgtaataaca agagcaacag
     2101 ttactgatag agttaaggaa ggtgcaacat atatgactta tcagtggtgg gttggagctt
     2161 gcaatgaact tacaatagca aatttagacc ctatatctaa aacaccagag tataaatatt
     2221 gtgctgttaa acttgaaaaa cttgaagacc aagaattagc agaaaaatgt gtaagagaag
     2281 agtatcaaag tttgaaagac aaaatgacag ctacaaatat ctaatgattt atttgattta
     2341 agatttataa aaggcagaaa ggctaatatt ttatgaacaa tatatactca aaaactaaat
     2401 tacaagaaaa tagtaaggat aaagtttttg aaaatagtta tagtctagtg gactacaaat
     2461 atttaaattc ttttaatgta gaagtaagca aaataaatga acatgaagta tcaatagata
     2521 atgaagaaat aattgcagaa tatccactta gttttatttt aaatgataaa tatgtaaata
     2581 catttttatg taccccatat aatttagagg aacttatagt aggattcttg aaaacaaaag
     2641 gatacataga aaacaaaaaa agcttattaa aatttgagat aaatgagagt gaacgttttg
     2701 caaaagtaac tattcaagag aaacaaaaaa atcaagatga gcaaattata tttttaaatt
     2761 cactagacta tataacctgt gctcctgtaa agagcaatat aaaaataaat tatgatacga
     2821 tttacagtat aatggataaa aatttaaatt catcagaatt atttaaaaat actggtgggg
     2881 tacatagcgt atctgtattt aattttgata aaaaaatagt cacttgtgaa gatgtagcaa
     2941 ggcataatgc aatggataag gctattggat tttgtgtttt aaatgacata acattaaaag
     3001 ataaaattgt ggctgtaagt ggaagaatat cattggaaat gatattaaaa gcagctatga
     3061 ctcaaattcc tattgtagta tcaaaatcag caccgactaa tttatcaata gagctttcta
     3121 aaaaattaaa tataacgctt attggatttg taagaggaag acggatgaat atttatgcaa
     3181 atccacagag agttatcaaa aagtgagaat gaatatatgt gtactaaaaa agaaaagcaa
     3241 agatattcaa agtgtgaaat ctgtggagta ttaattgatg atactgataa ggtgtataat
     3301 aacacataca atttgtgcaa aaaatgtaaa cgaaatataa gtactgagaa aatagtgaaa
     3361 tatataaata tatatgatga aagtaagtag acatatgatg aaaataacta gatggttgga
     3421 aatatacaaa ttgaaaggtg ggataatatg aaaaataaaa taggctcttt tgtaatagca
     3481 gacccaaaca aatgtacagg ttgtagagct tgtgaagtag cttgttttac aatgcataat
     3541 cagcataata atgtgggtta tacagttgga acagttgata tacctgttat acctagactt
     3601 tacttagtaa aaggtgacaa tttctgtatg ccaatacagt gtagacattg tgaagatgct
     3661 ccttgtttaa actcatgtcc acaaaaagct atagttaaag aaaacaatat tatgtctgta
     3721 aatgaagaaa agtgtatagg atgtaaaact tgtctgctag catgtccttt tggagctata
     3781 gacttacttc cacaatatga agatggtaaa gaagtagaac aagtaatttt agatgaaaat
     3841 aagaagatag cttataaatg tgacttatgt aaagacaatg aaaaaatagc ttgtataagt
     3901 gcatgtcctc aggaagcatt aaaacttgtt actccaatag atgacaaaaa agctaagaat
     3961 agacaagctg cacttagttt attgttaaca aataaataaa ggatacaggg gtaggtgacg
     4021 tttatgataa taaatataga taaggattta tgtactggat gtagagaatg ttcaaaggtc
     4081 tgtccagtaa atgctattga aggtgaagag ggaaagccac aagagataaa tttagataga
     4141 tgcgttatgt gcggtcaatg tgttcaaact tgtaaatctt atgcctcagt tatagatgaa
     4201 ggatttgagt ttttacaaga gaaaaaacaa gaaagagaga taccagagtc tataaatgaa
     4261 cctatatttg cagcatacaa tgtatgtgat atagataagg tgaagaaagc tttaagtgac
     4321 ccaaatgtat tcactatggt ccaatgtgca ccagcagtta gagttgcttt aggagaagat
     4381 tttggatatt atttaggata cttaggtgct gggaaaatgg cagcagcact tagagcttta
     4441 aattttgata gagtctacga tactaatttt gcagcagatt taactataat ggaagaaggt
     4501 agtgaattaa ttaaaagagt gacagaaggt ggaactcttc caatgtttac atcttgttgt
     4561 ccagcttggg taaaattcat ggaaaaaaat tatcctaaat taactaatca tttatcttct
     4621 tgtaaatcac ctcagcagat gggtggtgca atatttaaaa cttatggtgc agagataaat
     4681 aatgtagatg catcaaagat atttagtgtt tctataatgc catgtacatg taaaaaatat
     4741 gaatgtgata gagaagaaat ggattctagt ggatatagag atgtagatgt agtattaact
     4801 actagagaac tagcttattt aataaaagac atgggaattg attttaaaaa tttaagagaa
     4861 gaaaaatttg atagtccttt aggtagttat agtggagctg gaactatctt tggtgttact
     4921 ggtggggtta tggaagctgc tataagaaca ggatatgagc ttataactgg agaaagtatt
     4981 cctgatgtag aagtaaaaca agtaagagga gaaaatggtt ttagaaagtc aactattaaa
     5041 ataggagact tagacttaag agttggagta gtaagtggat taaaaaatgt aataccagtg
     5101 cttgaagact tggaaaaagg aaaattagat gtagatttta ttgaagttat gacttgtcct
     5161 gttgggtgtg taagtggtgg aggtcaacca aaaattttac tagaagaata taaggaattg
     5221 gcttatgaaa atagaacaaa agcaacatac gtgcatgatg aacatttagc aattagaaaa
     5281 tctcatgaga atcctgatat taaaaaactg tatgatgaat atctggtaga accattagga
     5341 gaaaaatctc atcatctatt acacactaca tattgtatag gaaaggaagt ggctaagtga
     5401 tgaactattt tgttatagca gaccctgata aatgcatagg atgtagaact tgtatgatag
     5461 gatgtgtagt tgcacattca gatgaagata ttttttacca aaatccagat gaaataaatt
     5521 ttaatccaaa actttctgtc ataaagacaa aagaagtaag tgctccaata cagtgtagac
     5581 attgtgaaga tgctccttgt gcaaaggcat gtccaaatgg tggtatagtt agggttggta
     5641 acacaataaa aatcaatgaa gaaaattgta taggatgtaa gacttgtatg ttagcatgtc
     5701 ctataggtgc aatagacata gttacattaa aagatgtaga tgaaggaaag ctttgcttta
     5761 gagaaagaat gacagcaaat aagtgtgatt tttgtataga atcgccagaa gggcctgcat
     5821 gtgtaaatgt ttgtccaact aaggcattta ctatagttaa ggatgaggat atagatagta
     5881 aagttaaaaa taagagaaaa ttagctatat tgtaatataa aaaagatgga gttttattta
     5941 tgattaaaag ctccatcttt tttattataa tatttatata tctaaaaatc aattattgca
     6001 ttagtttatc taatctttct ttattcaaat ttttcattcc tatagaagtt ataataagta
     6061 aagtataggc aattataact gataaaatta taatagacca agaaaatctg tatccaaaat
     6121 tatcaactaa caatccaaat aatgttgaac ctaatgcaaa tcccactgca aaaattaatt
     6181 gtacgattcc atatatgcta ccaaactcct tttgaccaaa gaaagagcca gttaaataag
     6241 caggaccaat catataagca aatacagaaa gacctttcaa aatggcaaat acaaatgcaa
     6301 aagtagaaga tttatctacc aatagtaatg ataaagttgc tataagaacc aatacaaaag
     6361 atataataag tggctttgtt tgaccgaatt tatcaaacaa taatcctcca aataaattac
     6421 caatgagagc aaataaagca aatgtggagc ctactgttcc tattagaata ggatttagat
     6481 aatttctaag ataagttgga tattgtacag ataatgatga aacatatata ccaacaaata
     6541 taaatccaat agtcataatc caaaaatatt taattttttt agcttctttt aatgtataac
     6601 cccatactga aggagaaatt gtatcagatg ctttctcttt tttaccttta gattttacct
     6661 tttcattaat atctttaggc atcctgagta taaataatat tacaggtata ccaactatta
     6721 atgctataat tccaaatatt agataagaac gactgtatcc ttgagttgct aataagcgaa
     6781 ctgcaagtgg ttgtagaaat atactaccaa tagaaccacc agcaaaagca aagcccaaag
     6841 cttttccttt gctttcttca tcaaaccaag cacttattaa aacaggtatt cctatagcag
     6901 atataattgt agcgccaatt tgaacaatac atgatacagt gtaaaattgc catagttcag
     6961 tacatataga aaaagtagca aaagcaatac tagaaattat acaacctaat aaatatagta
     7021 tcttaatatt gactttagaa aataatacac ctataaaagg tgatgcaatt gctgaggcaa
     7081 tagtaccaat agtgaatatt agagaaaaag aagctaaact aaagccttct ccttctacaa
     7141 cataacttat aaattgaggc tgtatacttg ctacaatact aaatggaaca gcttgtatca
     7201 acatacaacc aagtactata acccaagcat ttgaaaagtt tttaatgcta aatttattgt
     7261 ttagtaatgt attcatggta tctctccttt aggttatgat ttgtagatat taatctaatt
     7321 attctaattt tatatattag ttttggatag cctaactatt taaatataat ttgggtatta
     7381 atataaaaat taactataaa aattgattat tttaaatgta tggggtaaat taaagtatat
     7441 aaataaatga aaaggatgga taaaatgtca tttgaaaaaa taaataataa taacctagat
     7501 aaaatacaca acaggaagtg tattatgctt gtaaacttta ataaaaaaga attatcatta
     7561 attaaaaacg taagtaactt tgctggaata agagaccata tcatcttaag tgataaaaat
     7621 ggagagaata taataagaga cattttagat ggaaatatat caaatgaatg tgaaaatggg
     7681 attaagagta agtctgtaat atttaataac actccaagca cacaagtaaa tgcattttta
     7741 gaagggctaa aaaaagtgag aataaataga ccttttacag ctatggtaac agagacatct
     7801 attgattgga cattgaataa tttaatagaa aatttagttc aagaacgtaa tgctatgtct
     7861 ttaggaaaaa caattgaaca taagtaaatt taagatatct tatagtaatt ttaactaatg
     7921 tacattgagt atcatattat attttagaat ataaaatttt aaatgtgggt agatggagga
     7981 gatttgagat gaaagtttta tttacaataa attatggaaa agaaaaattt gataaaataa
     8041 gagaattagg ctatgatgtc atacattact ctgaagatag tattgaaaat aatgaggatg
     8101 taaatacagc agatgtacta gttacataca atccatttga gagattagat atttctaaga
     8161 tgaaaaattt acaatatata cagacaacta gtgtaggtat agaccaactt ccaaaagaag
     8221 aggtattgaa gagaaatatt aagatagcta ataatagagg tggatatagt gtacctattg
     8281 gtgaaagtat tgtattgtat attttagaaa tatataaaaa tagtgcaaaa ttttttaaac
     8341 aacaacaaaa taaagaatgg aagatggatt tttctgtatc agaattgtat ggacaaagag
     8401 ttggattttt aggaacaggt actatatctt cagaagcagc aaaaagatta aaagcttttg
     8461 gtgtagaaat atggggtgtc aatactgatg gaagtagtaa aaaatacttt gataagtgtt
     8521 ttgctactga ggatatgaat attgtatttg aagaatgtga tatagtagtt gtagcaatgc
     8581 cttctacaaa aagtacagat ggaatagtaa acaaagatat gtttaaactt atgaaagatg
     8641 gttctgtatt tataaatgta ggtagaggaa atacagttaa tcagaaagat ttagaagaat
     8701 gtgtagagaa atttagaggg gtagcattag acgtatttga aagtgaacct ttaaacaagg
     8761 acaataagtt gtgggagtgc gaaaatgtca tagtaactcc tcataattct tgggtgtcag
     8821 ataaaaacaa agagagaaca tttaatatgg tttataataa tttgaaacac tatataatgg
     8881 agaataaacc tgtagataat gttgtagaca tatcaagagg ctattaaaaa taaaaaattt
     8941 tgataaaaat aaaaactctt attagtcgta ttaaagatac taataagagt tttttttgta
     9001 ataaatttaa agccaaaaaa gtgatatact aaaaaatata taaataggag ataattaatt
     9061 atagggaaat ttcaaaataa tttaatttta aaaatatgaa gaggtgcaaa aagtgagcgt
     9121 aaaagatgaa attaataaac ttatagagac aataaactac cataatgaaa ggtattataa
     9181 tcaagatagc ccagaaatat cagattatga atacgataag ttaatgaagg agttaatttc
     9241 tttagaagaa gaacatccag aactaaagag aattgattct ccaacaaata gggtaggagg
     9301 taagccatta gataagttca atcaaattgt acataaaact cctatgttga gtttatcaaa
     9361 tgctttttca gagcaagatt tgagagattt tgataagaga gttcaagaat atgtgggaga
     9421 aaatgttgaa tatgttgttg aatttaaaat agatggtctt tctgtaggtc taacttataa
     9481 caatggagag tttgaaaaag gagcaactag aggagatgga gttgtaggag aagatatttc
     9541 tcaaaactta atgacagtaa aaagcatacc tcttaagata aatgataaaa aagaactaat
     9601 agttagagga gaagtatata tatcaaaaga taattttaga aaagtaaatg aacaacaaga
     9661 agaacaagaa cagccattat ttgctaatcc aaggaatctt gcagctggtt cattgagaca
     9721 attagaccca aaactaacag caaagagacc attagatata tttgtattta atatggaaaa
     9781 tatagaagaa tacgatttag aaagtcacag tgaatcacta gagtacttag aaaaactagg
     9841 attttcagta agtcagagtt acaaagtttg taaaagtata gatgaagtta ttgagcatat
     9901 tgaatactgg acagataaca ggggtaattt agactacgaa atagatggta tggtaataaa
     9961 agtaaacaat ataaaacaaa gagacgaaat gggatataca gccaaaagtc ctagatgggc
    10021 aattgcatat aaattcccag cagaaaagaa gaaaactaaa atagtagata taatagtaga
    10081 agttgggaga actggtacaa taacaccttc agcaattctt gaaccagtca gacttgcagg
    10141 tactactgta agtagagcta cacttcacaa tgaagattat ataagagaaa aagatataaa
    10201 aataaatgat acagtattag ttcaaaaagc aggggacata atacctcaag ttttagaagt
    10261 tataaaagaa gaacgtactg gagatgaaat agattttaaa atgccagaaa agtgtccagt
    10321 ttgttcagag ccaacaataa gattagaagg agaggctgct gtaaaatgta tcaatatgtc
    10381 ttgcccagcc caaataagaa ggggaattat acactttgtg tcaagggatg caatgaatat
    10441 agatggattg ggagaatcta taattacact gctattaaat gaaaaaatta ttcaagatgt
    10501 atctgattta tattatataa aaaaggaaga tgtagtcgac ctagagagaa tgggcgaaaa
    10561 atctgctgac aatttaataa attcaattga aaaatcaaaa gaaaatgatt tatggagact
    10621 tataaatgga ttaggtatta aatttatagg agttaaagca gctaagatac ttgcatataa
    10681 ctttaaggat ttagatgaag taatttctgc aactagtgag caactagaag aattagagga
    10741 atttggaagt ataatggcaa acagcgttgt cgagtttttt aaggaagaca aaaatatgaa
    10801 cgttataaac aagttaaaaa atgtaggagt aaatacaaaa tcattggata atacaggtga
    10861 aaaagtagaa aaaatatttg aagggatgaa aatcgtattg actggtacac ttcctacatt
    10921 aaaaagaaat gatgcaaaag atatgataga aaaaagaggt ggaaaagcta catctagtgt
    10981 tagtaaatcg acaacatttg ttttggcagg tgaagaagct ggttctaaac ttactaaagc
    11041 aaatgattta ggaattaaag ttatagatga agagagattt ttagaattat taaagttatc
    11101 atcgaaagat gaagtaaaag ctgtattaga gagttaaaaa gtcgcttaat ttagtagaaa
    11161 actactataa ttaatgatag aatagttcag taagctaata agattacgaa aaatgacaat
    11221 aattaatatt tgacaaaatt aaaaattata aaaataaatt tataaaaaat aaaaaatatg
    11281 agaaaggata taaaaaatga ccagaagtta tgatagaagt aatgaacaga taagacctgt
    11341 aaagataact agagattata caaagtatgc agaaggttct gttcttatag aaatgggaga
    11401 gacaaaggtt atatgcactg catcagtgga agaaaaagtt cctccttttt taagaaatag
    11461 tggtactgga tggataaatg ctgaatattc tatgctacca agagcaacac aacaaagaaa
    11521 gataagagat gcatctaggg gaaaaattga tggaagaaca caagaaatac agaggttaat
    11581 aggaagagca ataaggtctg ttgtagactt gaataaatta ggagaaagaa ctatttgggt
    11641 agattgtgat gtgattcagg cagatggtgg aactagaact gcatctataa caggggcatt
    11701 tgtagcagtt gcagaagcaa tatataaatt atataaagca aagacaataa aagagatgcc
    11761 aataacaaat tttgtgtctg caattagtgt tggtattgta aatggaatac acatgctaga
    11821 tttatgttat gaagaagatt ctaaagcaca ggtagatatg aatataataa tgactgataa
    11881 gtgtgagttt gtagaagtac aggggacagg agaagagaaa cctttttcga gagcagattt
    11941 aaatttattg ttagagttag gtgaaaaagg gaataaagaa ttaataaggc ttcaaagaga
    12001 agctttaggt gaaatatcag atgagatatt gggtatggat tatggtaatg aagtagtgat
    12061 tgctactaat aatgctcata agctagaaga aataggtgaa atcttaaaag attttgaata
    12121 taaagtgtac tctttaaaag atgttgattt agcaggtata gagatagtag aagatggaaa
    12181 aacatttgag cataatgcgt tgataaaagc tagaacaata gcaaaaaaga ctaaacttat
    12241 agcaatatca gatgattctg gcttagaagt agatgcttta ggcaaaaaac ctggtgttta
    12301 ttcagctaga tatgcaggag aacatgcaac agatgaagaa aatagaaaaa aactgttaaa
    12361 agctatgcaa aatataccaa tgagcaaaag aaatgcaaga ttcgtctctg caatagcagt
    12421 agtttttcct gatggaaaag aatttgtagt aagaggaatt tgtgaaggaa tgataggatt
    12481 tgaagaaaaa ggtaaaaatg gttttggata tgatagctta tttatagtaa aaggatatga
    12541 taagactttt ggtgaaatac ctagtgttat aaaaaattca atcagtcata gagcaaacgc
    12601 actaaaactt atgaaacaag agtttataaa aagggtagtg aagtaatgaa aataggcata
    12661 gtaagcgaca cacatatgat gattaaaaat atggaaaaaa caataccata tttgaaagaa
    12721 tgtgacctaa taattcatgc aggagataac tttactgatt caagatatat tcatagcatg
    12781 actaatgtag gtattattgc agttaaaggt aattgtgact ttgatgctgt tgaggaagaa
    12841 gttgtctttg aagtggcaaa taaaaccata ttcttatgcc atggagataa gtatggtgta
    12901 aaatatggaa ctaatatgtt agaaaaaaag gcaactgaag ttgatgctga tatagttata
    12961 tttggccata cccatacccc atttagagaa attaaagatg gtgtacttta cataaatcct
    13021 ggaagcactt cacttccaag aggagtatcg tataaaagtt ttgtcattat ggatatagaa
    13081 gaagatgaca taaaaattga agaaatacgc atataaaggt gtatttatct aaaaaaatgg
    13141 caaaaaacgc tcaaaaaatt aaattttatc aaataagtac aacgatatta atggaacact
    13201 tgtaaataaa aaaacttatg tgatataatt aagtggttga attataaata gaatgaatta
    13261 taataccgtt atcgggtatt acataaatat cactaggagg attagaagga tgaaagcaga
    13321 actaattaaa aaagagggta ataaagttac tttaaaaata acagtagata acaataaatt
    13381 tgaagcagca gtaaataagg cttataataa aagcagaaat aaatacaata tacctggatt
    13441 tagaaaaggt aaagctccaa gaattgttat agagactcaa tacggaaaag gtatattcta
    13501 caatgatgct atagaaatat tattcccaga agtttatcca gaagctatca aggaattaga
    13561 catagaccca atagacaatc cagatttaga tatagaagaa ataagtaaag acaatgggtt
    13621 ggttatggtt cttaatgtag aagttaaacc agagtttgaa ttaggaaact acaagggtat
    13681 agaaatagct aaggttgaga atactgtaag tgacgaaaat gttgatgcta aactacaaga
    13741 aatggttgaa aaaaatgcca gattagtaag tgtagaagac aaagctttag aagatggaga
    13801 tacagctata atagacttcg aaggttttga aaatggtgta gcttttgatg gtggtaaagg
    13861 tgaaaattat aacttagtta taggttcaaa cacatttata ccaggatttg aagaacaatt
    13921 agtaggtaaa aaagctggtg aagaagtaga agttaatgtt actttcccag aagaatatca
    13981 ctcacaagat ttagctggaa aaccagttgt atttaatgta aaaataaatg atgtaaaagt
    14041 aaaagaatta tctgcacttg atgatgaatt tgctaaagat actagtgaat ttgattcttt
    14101 agatgaatta aaagcagatg taagagctaa gttagaagaa gaagctaaaa atagagcaga
    14161 tgcagaaact agaaattcag tagtagaaaa agttgctgaa aatacagaaa tagaaatacc
    14221 agaagttatg atacaacatc aaatagataa tatgttaaat gaacttaact accaattaca
    14281 atatcaagga tttggattac aacaattact agagatgact ggtagaacta tggaagaatt
    14341 aagagaagag agaaaagaag atgctaaaaa attagttaag tcttctttag tattagaagc
    14401 aataactaaa gctgaaggaa ttgaagcaac agaagaagaa tttaaggctg agttagaaaa
    14461 aatggcttct gcttacaata tggaagtaga aaaaatagaa gcttctttaa gagatgctga
    14521 caaagaagat ataaaaggtc aaataaaaat aagaaaaaca atagatttat tagttgataa
    14581 tgcaacaata gcttaataaa ataaacgcta actaatatag ttgacgttat gaaatgttta
    14641 gtatataatg atgtgcaaat ataatttagt taaatgcgta taataattta cagtagctta
    14701 aaaaaagcta ctgtaaatta tatagaataa agggggtatg aaaccatggc attagtacct
    14761 gtagttgttg aacaaacagg aagaggagaa agatcttatg atattttttc tagactatta
    14821 aaagacagaa taatcttctt aggagatcaa gttaacgatg ccacagcagg gcttatagta
    14881 gcacagctat tatttttaga agctgaagac ccagacaaag atatacattt atatataaat
    14941 tctccaggag gaagtataac ttctggaatg gctatatatg atactatgca atacatcaaa
    15001 ccagatgtaa gtactatatg tataggtatg gctgcttcta tgggagcatt cttgttggca
    15061 gcaggagcaa aaggaaaaag actagcacta ccaaatagtg aaataatgat acatcaacca
    15121 ttaggtggag ctcaaggtca agcaacagat atagaaattc atgcaaagag aattttaaaa
    15181 ataaaagaaa ctttaaatga gatattatct gaaagaactg gtcagccatt agaaaaaatt
    15241 aaaatggata ctgaacgtga taacttcatg agtgcactag aagccaaaga atatggttta
    15301 atagatgaag tatttacaaa gagaccgtag atagagagag gtgttaatat gtctaaatat
    15361 gaagaaaaac gacaattaaa gtgttcattc tgcggtaaaa atcaagatca agtaagaaga
    15421 ttaattgcag gtccaaatgt atatatttgt gacgagtgtg ttgaactttg tgacgaaatt
    15481 attcaagaag agatagaaga tactatagat gaggatacta catctttacc aaaaccaaag
    15541 gaaatgatgg agattctaaa tgattatgta ataggtcaag aaaaagctaa aaaagcttta
    15601 tcagttgcgg tttataatca ttataagaga atatacagta aaaaatctag tagtaaggat
    15661 atagaaattc aaaagagtaa tatactttta ttaggaccta caggttctgg aaagacatta
    15721 cttgctcaaa ctctagcaag aactctaaat gtaccttttg caatggcaga tgctacatca
    15781 ttgactgaag ctggatatgt tggagaagac gttgaaaata tacttttaaa acttattcaa
    15841 gctgctgatt ttgatataga aaaagctgaa agaggtataa tatatataga tgagatagat
    15901 aagattgcaa gaaaatctga aaacccatct ataactagag atgttagtgg tgaaggtgtt
    15961 caacaagcac ttctaaaaat attagaaggt actgttgcaa atgttccacc acaaggagga
    16021 agaaaacatc ctcatcaaga atttttaaag atagatacta ctaatgtatt atttatatta
    16081 ggtggagctt ttgatggatt agagaagata attcaaaaac gtggtggaga taaaacacta
    16141 ggttttggag ctaaaataga aagtaaaaaa gaattagatt tagggaagct atatgaaaaa
    16201 gtacttccag aggatttatt aaaatatggt ataataccag agtttatagg aagaatacca
    16261 gttttagcta ctttagagtt gttggatgaa gatgcattaa tgcagatatt acaggaacct
    16321 aaaaatgctc tagtaaaaca gtataaaaag ttattggagt tagatgatgt tgagctagaa
    16381 tttgaagaag gtgctcttcg tgcaatcgct aaaaaggcca tagaaagaaa tacaggagct
    16441 agaggactta gaagtatagt tgaaagcgta atgatggaaa ctatgtttga agtaccatct
    16501 agagataata taaagaaggt tatagtaaca gagaaatctg tgaacgaaga ctcagtaaat
    16561 cctatcatag tacttaagga tcaagaggaa agtgcataag atgatgaaaa ggagctttaa
    16621 aagctccttt ttttatgtta atatgctaaa tataaaatag gtatcattat tataaaaatg
    16681 tgagtaatat acctagaata ccaaagacga atacaattgc aatggaacca atttttcctt
    16741 tatataaaag ataaaatgat gctataaatg caattattcc atataaatcc caattaactt
    16801 ctttggcaga tgaaaaagct gctgataata tgaatcctag agtaattggt ctaagaactg
    16861 tcaataagtt ttctaccttt ttattatttt taaatttaga aaataacttt gatataatag
    16921 ttagacctat taatgcaaaa attgtaacac caatagttgc agctagagaa ccaaagaagc
    16981 cagcagtctt aaatcctaca aatgtagcac tatttattgc tacaggacct ggtgtagatt
    17041 gtgatatagc aagtaaatcc aaaaactctt gattagttat aaagtgatat ttagatataa
    17101 attcttgctg gataaatggt agcatggcat aaccgccacc aaaactaaat gcccctattt
    17161 taaggaatat taagaagagt agtattattt ctgacataga ctatatacac ctcctccaat
    17221 tattaaataa atagggctta tattgaatac accaacagat aaaactgcta taattaatag
    17281 tagtatgtta gttttagact ttggtagttt cttgaacatg ctcgtaaaag aatacaatat
    17341 aagagctatt acaacaggcg caactccttt aaaaaatcca tctaaaattt tagatgatga
    17401 atttttaaag tatagatacg ataagactaa gactatacaa aatgatggta aagcagagcc
    17461 taaggtacat ataagaactc ctggtatacc ataaagttta tatcctaata atacagatat
    17521 attaactgcc agtactccag gataactttg agctatggat aaataatcaa taaattcttc
    17581 ctgctctaaa agtttttgtt tgtttacaag ttcatcttgt ataagaggaa ccatagcgta
    17641 tccaccacca aatgtaaacg caccaatttt taaaaaggtt aaaaacaatt tcaacataat
    17701 agaaacctcc aaaagataaa taacatctaa gtatactaat attaatatta tatcataata
    17761 aaaagataat atagtatgat agtattctaa gaatttatca ttaatgttga aaattaactt
    17821 gttcaaatat ataatattaa aagcaaagca attagactag aaaaggagta atgttgatgg
    17881 aacaaaatta tactaaaata gatcatgaat taccattaat tcctctaaga ggattagcta
    17941 tatttccata tatgatatta aattttgata taggtagaga gatatctctt aaagctttag
    18001 accaagctat gatggatgaa gaacttatat ttttaacttc tcagaaagaa gcagaggtag
    18061 atgagccagg agaagaggat ttttatcatg tgggaacaat ctgtaaagtt aagcaaatga
    18121 taaaattacc aggagataca gttcgtgttt tggttgaagg tgtatcaagg ggtagagtaa
    18181 agaaaataga acaagaagat gggtatttca gagcagtaat agaagaaata gtatttgata
    18241 gtgataattt ggatagtgaa acagaagttg aaattgaagc atttgtaaga aatgtatttg
    18301 atgcctttga agagtatata aatattggaa atagggtatc accagaaata ttaatatctt
    18361 tagctgatat agaagatgta gatagattta tagatacgat agctgcaaac atatatctaa
    18421 aatcaagcca aaaacaagaa atattagaag aatttgatat tagaaaaaga cttgaactta
    18481 tttattctat attattagag gaaatagata tattaaaaat agaaaagaaa ataactttaa
    18541 gagtaaaaaa acagatgaat aaggttcaaa aagaatatta tcttagagaa caattaaaag
    18601 ctatacagaa agaattaggt gaagaagaag atataaactc tgaagcagat gagtatagag
    18661 aaaagcttaa aaaaataaaa gcacctaaga caactaaaga gaaaatagaa aaagagatag
    18721 ataaattttc aaaaatatct tctatgtctc cagatgtatc tgtaagtaga aactatttag
    18781 atactatatt ctcattgcca tggaataaag agacaaaaga taaattagat ataactaagg
    18841 caaaagatat attagatgaa gaccattatg gattagaaaa agttaaggag agaatactag
    18901 agtatctagc tataagaact cttgctaagt cacttaaagg gcctataata tgtctagttg
    18961 gtcctccagg aacaggaaag acatctatag taaaatcaat cgccagagca ttaaatagaa
    19021 aatttgtaag aatatctttg ggtggagtta gagatgaagc agaaataaga ggtcatagaa
    19081 gaacttatgt aggttcaatt ccaggaagaa taattaatgg ggtaaaagaa gcccaaacaa
    19141 aaaatcctgt attcttattt gatgagatag ataagatggc agcagattat aaaggtgacc
    19201 cagcttcagc aatgctagaa gtattagacc cagagcaaaa taaggatttt gtggaccatt
    19261 acttagaaat accttttgat ttatcaaaaa tactatttgt tacaacagca aatagtctag
    19321 gtaatatacc aagaccatta ttagatagaa tggaagtaat agaagtatct ggatatatag
    19381 aagaagaaaa attaaatata gcaaaaaaat acctacttcc aaaacagatt aaagaacatg
    19441 ctttaaaaga aaactttata aaaatagatg atgaaacttt aagaagtata atcaaccatt
    19501 atacaagaga agcaggtgta agaacactag aaagaactat tggtaagatt tgtagaaagg
    19561 ttgccaaaaa atacgttgaa gacccaactc ttgaagaggt tgttataaat aagtctgacc
    19621 tagagacata tcttggaaaa gacatgttta agtatcaact tgcagaagtt aaccctcaaa
    19681 taggattagt aaatggtctt gcgtggacag aagttggtgg agttacactt gaagtagaag
    19741 taaacgtact taaaggtaaa ggcgaaattg tattaactgg aaaattagga gatgttatga
    19801 aggaatctgc gaagacaggt atctcttata taaggtcaat agtggacaag tttgatatag
    19861 accctgaatt ttataagacg aatgatattc atatacacat tccagaaggt gctgttccaa
    19921 aagatggacc atcagctggt ataactatgg ctcttgcagt aatctcagca cttactaaaa
    19981 gacctgtgcc tggaaatata gcaatgacag gagagataac tttaagaggt agagtcttag
    20041 cagtaggagg agttaaagaa aagttattag cagctcatag agctggtata acaaaagtat
    20101 taattcctaa agaatgtgaa gctgatttag atgaaatacc agaaaatgta aaagaaaaaa
    20161 tggagtttgt acttgtagaa catatggatg aagtattaga acaagcttta ttaaagagtg
    20221 gtgaaaataa tgaaaattag aagttcagag ataactatga gtgctgtgaa taaatcacag
    20281 tatccagcag aaggaatccc agagatagca ttggctggta ggtcaaatgt tggaaaatca
    20341 tcaataataa atacattgct taacagaaga aactttgcta gaaccagtca aacaccagga
    20401 aaaactagaa ctataaattt ttatctaata aataatgaat tttattttgt agaccttcca
    20461 ggctatggat atgcaaagat agctaagtct gaaaaagaaa agtggggagg tataatggaa
    20521 cgatatctag aaagtagaca agagttatgt tctatattcc tacttgtaga tattagacat
    20581 gagcctacag cagatgataa gcttatgtat gaatggataa agcactttgg atataactgt
    20641 gtagttattg caactaaggc agataaaata tctagaggac aatatcaaaa acacataagt
    20701 ataataagaa aaaaattgca aatggaaagt agtgaaaaag taattcctgt ttcttcacta
    20761 aaaaagactg gtgttgaaga attatgggaa gagattgtaa atcaatacaa tcaacatgga
    20821 tatgaaataa cagtagatta aatttaaaat gttaaatatt gacataagat ttatttgaat
    20881 aataaatgta ataatagtta gttttaatta tattaatatt aaagcaaaat ataggatgcc
    20941 ttaaaatagt tttaatgaac tttttttggc atccttattg tttataaaca ataaatatca
    21001 gtaattttgc taaatactaa taaattttag taaggtatgg gaaaattagt ataaattagt
    21061 tcaactaaaa aattattgac ttatgtagtc agcaataaat agcatttaag aaatagattt
    21121 atctgttttt ctcataaaga aaacagttag tataagccct ataaaacata ctgatgctgc
    21181 aatccaataa gactgattgc tagctataac ataggctgat gtgtgacttt taaacttttg
    21241 attgtatgta agactttgtg caaaaaaact actaaaaaat gctgttccaa gagcaaatga
    21301 taaattacga atagttgcaa gaaaactgcc tccgtatccc tgtttttgag gaggaacatc
    21361 tgctaaaata ctactattgt ttggtgaact aaatgctcct agcccaagtc caaaaataac
    21421 taaacataca attaaaagat acaatggaga atctgctttt aaaagtgcca ttccaactag
    21481 tgagatggtc ataaatgaaa aagatgctgt tagaatgttt tttgcaccgt gtttatcaga
    21541 aagtgaacct gcaataggtg ctataagtac cataataagt ggatgtacag tcattacata
    21601 accagccatc ataggagata aattttttaa ttgttctaaa taaaatggaa gtagaaaaga
    21661 tactgccatt tgaggaaaat aacaagctac tccaatgata ttgcctacag taaagtttct
    21721 atttttaaat agaggtaggt caataaagct ttgttcaaat tttacttctc ttaatgccaa
    21781 caataagaag attataagtg ctgttatacc taaatacaat cttgtattac ccttctcatt
    21841 cattgcaagt aatataagcg tagtgcaaga tgcgaaaagt agaagacctg gaatatcaaa
    21901 gctcttgttt tgttcatcca aaacaggttt aggcagaaat ttaatgccaa gaaagactgc
    21961 tataatacca atagggatat tgataataaa aatagaaggc catccaaaat gttctaaaat
    22021 tatacctcca attacaggac cactcatatt tcctatacca actacagctc cagtgattcc
    22081 tatagctttt cctttttcat ttgctggaaa tgcattggaa actataccaa gcccagtagc
    22141 cattaaaata cttgctccaa ctgcctgaaa gattcttgaa gaaattaatg ttgataaatt
    22201 gtttgacata ctacacagaa aagaacctat tgtaaatata aaaaatccta atgtgtatag
    22261 acgatttgaa cctactttat cgccaagttt tccgaaaaat aacatagtag ctgttacaac
    22321 aagcatataa gctgttacaa cccactgaat ttgtgacatg ttaattttaa agtcagcagc
    22381 aattgtagga tttgcgatat ttagtatact tccatcaaga gtagacataa atacaccaat
    22441 acagatgata atcaatacta tccatttttg tttcatgata acactccaat cttaatagat
    22501 tttataagta cgatataatt gtaaaataaa caacaaaaaa agcacctgta tattaggtac
    22561 cttgaatgtg tctaatttca tgaataaata cggtagttga aggatttgta tagattactc
    22621 atgtaaaatt acataaaggt actcatatac tattttcttc taccatccag attttactgt
    22681 tggctttgga atatgaccaa attatgccat aaggctcgcg ggcttttacc gccgataggg
    22741 aatttcaccc gtttatgaag aaaaacagat gtattttgta atattatttt aacataaaat
    22801 tataatggaa acaattggat ttaagtaata tccagctgta ttacaataca aatatttcaa
    22861 tatggacact taaattgtat ttatagatag aataaggcac agtatgagaa tctataataa
    22921 ttagataaaa ttaacctgtt attagtatta gatggcaagt tttaaatttg tcaaaataag
    22981 aaagctgtaa taaaattctt ataatatgaa tatcttaatt aagagatatt gtaaattatg
    23041 aggaggcgct aatatggaag gaaaggtaaa gaaggcattc ccgggtggaa atacatcaca
    23101 aggctcatat tctttttttg actatataat tcctgaaaat gtaaaaagag ttttttgttt
    23161 aaagggtgga cctggagtag gaaaatcctc acttatgaaa aaaatttata aagagttttt
    23221 gcatagagga tatgatattg atttatatca ttgtccttct gacccaagtt cgttggatgg
    23281 tttagttata aagaaattag gcgtggtact tatggatgca actgccccac atactatgga
    23341 cccaaagcta cctggagctt tggatgagat aattaattta ggtgattact ggaatacaga
    23401 agaattagaa aagaataaac aagaaatatc agtttatgat aaagatatta gtaattcatt
    23461 taaaagagca tttaagtttt taaaatcagc agaaccaatc tttagagaca tagaagaaaa
    23521 atattctgat tgtatggatt atggcaaagt aaatttggtc acagaagaat ttataaaaag
    23581 gttatttgat ggagttaaaa gcacaggaaa tctaaaaaga gaaaagcatt tatttggttc
    23641 agctataact cctgtagggt gtttagatta tacagaaaac attttaaaag atgtaaagaa
    23701 agtctattat ttagatggag aaataggaac tgggaaaact acattattaa acaaagtata
    23761 taagaaagct attgaaaaag gtttaaatgt agaagtttat cattatccac ttattccaga
    23821 gaaaattgaa actgtactat taactgattt agacataggt ataactataa gtacgacttt
    23881 taaagaagaa gatacaattg atttaaatca gtttttaaat agagaaaaac tacttaaata
    23941 tgaagaagat attaaatttg atgaaaaagt tttagatgat ttaatttgtt gggcagtact
    24001 aaatcttaag agagccaagt ctaaacatga tattatagaa agttactata gtcctaatat
    24061 gagatttgat gaagtaggga aattaagaga taaattaatt aaaagaattt taaaatatga
    24121 ggaaaactta taaacgtata tgtttaaaat taatgttaat aatgatataa tttttagtaa
    24181 attggtgtag ctttgaggct atgccatttt actttttttt gctaaaaaat gatattaata
    24241 atctccaaag caattatttg tttttcataa ttattatttc ctttcctaat ttatattttt
    24301 ttataatatc aaaacaatgt ttattgaaag tttatataat tttaagaaca tctaaaaatg
    24361 catacttgat attggtaaat tagtgatagg agttcaatgt atgatattat atactaaaga
    24421 tgaattttaa tatataactt tgatataata agcatatgta aatgtttaat atttaattaa
    24481 agaaaattat catataaaaa taaatttgta aggagcaata cagtatgaaa aaacaatatg
    24541 tgtttataat actaacaagt actattttag gtatacttgt tggaaccata tttagagatt
    24601 atagcaatga agaaacagtt tatacaagca aagacaagtc aataagaaaa gaaataaaaa
    24661 tgacaagaaa atctataaaa gaattaacca aagagaaaga caagttagat aaagaaatta
    24721 attcaatgaa acaaaatcaa aaaaattctg aagaaatatc agaaatagaa acacttagag
    24781 atagtctatc atattcagat gtaaagggta gtgggataat attaagtata gatgctatag
    24841 atgaaaaaac agggaatata gctaatagta tagactataa taagattctt gtaaaagtta
    24901 taaatgaatt aaaagtaaac ggaggaaaat acatagaaat aaatggtcaa agaattaatc
    24961 aatattcaga agttgtactt gcaggaagcc atataaatat aaatggagtt cctgtagcac
    25021 aaccatatga aataaagtgc attggagata aggagaaact atctaattat gtagataaag
    25081 gtagtaaata tttagatagc atacaaacca attatccaat gaaaataagc ataaaaactg
    25141 aagaaaatat ctctatgaaa aaaatgaata tacctaataa attaaagtat ataaagggtg
    25201 attaagatta tattagagga aagcagtttg ccaatgttaa taccagaaaa acttgcaaaa
    25261 aaatataatg tatttccagt tgagtttcaa gatgataatt tagttgtaga aatagaagat
    25321 gaggatattt atgctctaca ggacttgaga ttagctacag gtaaagaaat tatattgaag
    25381 aaggagaaaa aagaacttat aagtgaaaag attgaaaaat attataatag ctttgaactt
    25441 gatgaggatt atgcaaaaaa attatttgaa aatcttttag aaaaagctat aaaagaaaac
    25501 gcaagtgata tacatataga accatttaaa gaacatttaa tagtgagaat gagggtagat
    25561 ggagaactaa aggaagtatc aaagttttta gttgatgtct atccatcatt gtctacggtt
    25621 ataaaattaa aggcatcaat ggatataaca gaaaaaaggc ttcctcagga tggtagggtg
    25681 gatataaagt taggtggaaa tttaattgac attagagttt catcaatacc aactgtctat
    25741 ggtgaaaaaa tagttttgag gatacttaat agagatactt ttttaaaaga caaattagaa
    25801 cttggttttt cagaggaagc tataaaatct ataaatcgta taataaataa aagagcagga
    25861 atattattag tgacaggtcc tacaggtagt ggaaagacta caacagtata ttccttatta
    25921 aatgatttaa aaggaataaa taaaaatatt atgactatag aaaatcctgt agagtataag
    25981 atggatggca taagtcagat tcaagtaaat tcaaaagtag ggttgagctt tgatgtaggt
    26041 cttagagcga tattaagaca agacccagat ataataatgg tcggagaaat aagagatgca
    26101 gaaacagcta agatagcagt aagagcagct ataacaggtc atttagttat aagtacttta
    26161 catactaatg atgcagtatc ttccatagct agattactag aaatgcaaat accaccatat
    26221 cttttaaatg cctctcttat aggtgttata tctcaaaagt tggttagaaa agtctgcaat
    26281 cattgtagcc atgagattat gataaaagat aattttagtg gagatgtaaa tactaaggta
    26341 gctgtagggt gtgaaaaatg taatgataaa ggatactttg gaagaacagc aatttatgaa
    26401 atcttagaaa ttaatgatga tataaagaca tgtataagaa atatggagga ttctagtact
    26461 attaaagatg tggcacaaaa taatggaatg ataacatttg aagatagttg taaaagacta
    26521 ataaatgaaa aaataacgac attagaagag tgtttagtag taaatgaaat gatttaactg
    26581 aattgtatag ttaaacttcg tttggtcagt tgtctttatt taatctattt taattaagat
    26641 gtttgagtat aaattatatt taagtgaaat aaaattaaat gcattaaatt agaaaatatt
    26701 aagtttattt aattgtaatt aatataattt ggaaggtatt atagtacatg agattatcta
    26761 aaaatatatt ttataattta gaaaagtttt atttagtatt aaatagaatc agtgataaag
    26821 aaattagagt aatttgcaaa gagataggca tattgctgga atctggatgt gaaattacaa
    26881 aaatatttga gatcattgag agtcagtcaa gtaaaaaagc aaagaatttg cttagtatag
    26941 tatcaaatca tattcaaaaa ggtaactcaa tagcagaatc atttcaaata acaggtatat
    27001 tttctaaatt ctttataagt atgataaaag caggagaaac tagtggaaac ttagatataa
    27061 taatgagtga tttatctaat tattatgata aagaatacaa attaaaaatg aaaataataa
    27121 ctatttcaat atatccaata atacttataa tattatctat cttatcaatg ttgtttatat
    27181 tcgtatttgt aatacctaat tttcaggtgg tttttaccaa caatggaatt gaaccacctt
    27241 taataactag agttttaatg ggaatatcta cagtggttac aaataactta gcttatataa
    27301 tatttagttt tatattgttt tcaataggaa gcatttattt ctttataaca aatgataata
    27361 taaaaaaatt gattaattcg ttaaaattca aaataccttt tataaaaaaa attaatcagt
    27421 tagtagctac aacaagattt tcaagggcat tgtccgtttt aattagcagt ggaatacaaa
    27481 ttgtggaagc tatagacatg tcagctagtg ttatagacaa cgaatttatt tatgaaaggt
    27541 taaagatttc caataattat ataaaaaaag gaaatagcat aggtaaatct ttgaatttag
    27601 caaatgtttt tccaaaatta tttatttcaa tggcaaatat aggagaagaa agtgggaaac
    27661 tagataaaag tctaaatgta ataaataaat tttatgaaaa tgaattaaat aacaaaatag
    27721 agcaaataat gaaatttata gaacctatgc taattgtatt tatgggatta attattggta
    27781 cttttatttt agcaatggtt gttcctatgt ttgatgcaat tactaatttt tagagagaaa
    27841 aataaatttt attagatgaa acttttgaaa aggagagaat atttaattga taaatttgat
    27901 aaataaaaaa cgaaaaggat ttacacttgt tgaaatgatt gtagtagtaa ctattttagg
    27961 cgttatatct agtatagcat tagttaagta tagtaaggtt caagaaagtg ccaaattaaa
    28021 tgcagactat acgaatgctg ctaatatagt aaccgcagct agcatggcaa ttaatgatga
    28081 tgaaaagaca atagactctc taagtgtaga gacattgaag gaaaagggat acctaaatac
    28141 tgttccagtt cctcagagta catcaggtaa attcgaactt gtcataaatg atagcggaac
    28201 agatataagc gtaaatataa attctaagca attttatcca aaataatatt ttatattgat
    28261 tgctgtaagt tttaaaatta tttttattta agaaggagat ggctataatt aaaaaaattc
    28321 taaacttaga aataaatgat gattatataa ggcttgtatt tttaagaaaa ggtcataaaa
    28381 aagtttttat agataaatca attataaaag agattaactt tgatataaaa aatgatagta
    28441 attcgattga gaagattaag tatatagaag atgtagttag atttgtaaaa gaggagattg
    28501 gtaaatcaaa aatattatat aaaaatttat actttaacat tcaaattcag gatatagtca
    28561 ttagaaatgt tgaagttcta aatactaaga aaaaaagaga catattaagc atgattgaat
    28621 ttgaaataac acaatacatt ccaataaaca taaataacta ttcaataaaa tataaagtta
    28681 taaattctaa aaatgataaa ttgaatatac aagtaattct tttcccaaag agtattattc
    28741 aattaattaa gaacatttca gaaaatctaa atatgaatcc aaagactata aatataaatt
    28801 ttgatatatt acaaaagctt ataaacttaa atttaataga gaatttttca gaagaagggg
    28861 tatttataga atgtaaaaaa agtcagttaa ttataaatat aacaaaagat aaaaaaataa
    28921 gagaaacata cttgctatca aaagagggaa aatcatatga gttcataaaa agattattaa
    28981 gggattttaa gtatgtttat tactatggta tagaaaattg ttttattgaa gaatacttta
    29041 aatatacaga taaatctatt gaagtaaatc cattaagatt aaaagataaa gtaaaagtag
    29101 aaaataattt tgatgcagat ttagaagaca ataggataaa ttatataagt aatataggaa
    29161 tgattattta agtgaaagtg gataaaacaa taaattttct taatggattt aaaaaaacta
    29221 aaccatgtaa taaaatctat aaaataataa tagtcttaat tttaacgtgt attttattta
    29281 aatcatttat gtatgtgaaa tctataaatg aacttgaaga ttatataagt caaaataagc
    29341 tagtggataa atcaaagaat actttaccaa caacagataa aactagagag aaattttcta
    29401 aaataaaact taatgaaata aaaaaaatat atgaaacaat tggaataaat agtattacta
    29461 aaatatcgat agataaaaat aagctagaga tggaaggtgt gtgtagtgat ttagatgttt
    29521 taaacaacct aaggaaaata aagtacataa aatattatta catagacagt attgtaagag
    29581 atggagaata ttatagattt agaataaaaa gttagatggg tgattatttt tgagattaaa
    29641 taatagagaa aaaaatctac ttaaaatatt gttaatatta gttattatat tggcaagtga
    29701 tatagcagta agttatttgg aagaaaacct aactagtttg aaaaatgaaa agcaaaatat
    29761 aataaataaa ataaataatc aagataatta tcaaataaag gataataaac atatagatga
    29821 taaatatata gaaagtgttg aatcaaataa aaatgaagaa ttgaaaaata attttaataa
    29881 ctcattttta gataaaaaaa caaataaaga tatagtgtta ttagtagaaa atgaactgag
    29941 agatattgtg aatgtagaat atattaatca agaaagtagt ttagataatc acaataaaaa
    30001 acaagttaat ttagaaataa aagttaatgg agaacttgca aatgtgttga aacttgaagg
    30061 tgtaatgaag aagttaagac taaataataa acttgaaaag atagaaatta ataaattaaa
    30121 agtcaataat tttgatgttc aaaatattcc aaatgaatat aaacaagaaa aagacaatat
    30181 tgttgaatgt aaaatgacag ttagagtcat ataacaaaaa agaatagata aagtaaaaaa
    30241 ctattatcaa ttaataaaat tttatagagg aattagtttt acgataattc atttctacaa
    30301 gagggattgt tgatgatttt atcgaaatcc taattaaagt taatatattg taccaaaata
    30361 taagtgaatt taatagaata tttacttata ctatagattg attaataaaa aaatggtata
    30421 ataacaaatt gtactttaat ttaataataa gggagtggaa gtcatattgt tagggagaaa
    30481 tgagctatgc ccttgtggaa gtggcaaaaa atacaagaaa tgttgtttaa ataaagatat
    30541 tgtttcagaa agagcatgca gaaaaactga actttcacaa aaacaatata cagatttata
    30601 ttcaaagtta tatgattatt caagacagga taagtttaaa gaagaatgtg aaaaagctaa
    30661 agaaatgttt tatataatgc aaaatgatag tgtaaatgaa aaatttgaaa gattttttaa
    30721 tacttattta atacaagacc acataatgga aaataaaaag gttatgacag ttggttttct
    30781 tgaagaaaat ggtacaaacc taagtcaatc agaagtatca atattaaaaa gtatgtttga
    30841 atcatacgta agtatttatg aggtaaaaga agtttctgct ggaaaaatag tattaaagga
    30901 ctgtttaagt ggaaatgaac tttgtacaga agatgtaaaa cttctgagaa gttttaaaat
    30961 cggaagttgt atgatagcta gaatagttga tattgaagga actaacatat tgatagatat
    31021 aactataagt atatcaaatg aggttaaaga tataatttta aaggatatta tgaatctatt
    31081 caatcaatat caagatgtat acaaagatat gaagtcgttc ttaatttatc atacacatat
    31141 actatacaag tatatacaac agcttttaga cccaagtata gctgaatatt taaagagaga
    31201 aagagataaa aacgatacaa aagttaattc agaaaatgaa ttagctgaag aagaatgtaa
    31261 agttttagat actcttaaag aaaatgtaga aaaagaaaat atagatgcat gtatagagtt
    31321 ttggaatgaa tataagaaaa atcatgaaaa tataaaagga gctgaaaatg gttgggcagc
    31381 tgctgttgaa tactatgtta aaaaagaagc aggacaacct ataactcaag ttcaaatatc
    31441 aaagaaatac gatataagct ctagtacact aggaaagaga tacaaggatt taaaaatatc
    31501 ttaattaatt ataaaaagct ttaagtcata gataaagaat ctatagtata atatatgtat
    31561 aatgtttaga aagaggtatt aataatgagt cttagtttag gaaatgcaat tgctgtatca
    31621 acgaacttta taaaatctaa cccagctgta cctgtgtaca acggagaaga aatatatgca
    31681 gttatttatc atatagaaat aggatataat aatggtgtat cacctgatac caaacaatat
    31741 ataataatag actataaaga gtttgattta tctatggata aaaaaaatga actaataaat
    31801 attgctaaag agtactgtaa atctaaaggt attgtagaaa acgaaaacct tgaaataaac
    31861 cttttagaca atgaagaagc agagaacttc ttagaaaagg tgtttaataa tatgaaggtt
    31921 ataagaggtc ttataactaa aaaaggataa ataaatggac tatataggaa tagaaaatat
    31981 aacaccttat gaaaatacat atgaatttag tgtatatgaa tatgatgatg aaatcacctt
    32041 aggtagtgaa aagttatatg tatgtgaatt gagggttgta ttgattaaag ttaattctct
    32101 gtatgttgaa agattgcata aatcagttga agcaatggtc ttagtaaaaa acttgaaaaa
    32161 agatttagat aaaacacttg ttgtaaacaa aataaagaat tttgtgctag atgagatttg
    32221 ggtagaaaat ctagtaaaag agaatataga agttatattt gtagaaagct aggaataacc
    32281 ctagcttttt tatattacaa tctaagagag gaaaacaata tgttaagagt atctaatgta
    32341 aaacttggaa ttgataaaga tatatcttta ttaaaagatg ttatacttaa gaagctaaaa
    32401 ataaaagaaa aaaacttagt caaatatagt atatataaag aatctataga tgctagaaaa
    32461 aaaggaaaaa tggaatttgt ttattctgta gatgtagaag ttaaagatga aaataaaata
    32521 cttaactcaa aaataaaaga tgtaacaaaa ataaaggaaa tcagatacgt aaatgtacct
    32581 gtgggtgata aaaaattaaa gaataatcct ttggtaattg gtagtggacc agctggtttg
    32641 tttgcaggtc tgcttttagc tcaaatgggt tatgaaccaa tcatacttga aagaggtctt
    32701 gatgtagaca gtagaaccaa agatataagt gatttttgga caactagaaa attaaaaaat
    32761 aattcaaacg ttcaatttgg agaaggtgga gcaggaactt tttcggatgg taaacttaca
    32821 actagaataa aagatatacg atgtagaaaa gtattagaag agttagttca gtttggagca
    32881 ccagatgaaa tattatactc acataagcca catgttggaa cagatatatt aaaaaatgta
    32941 gttaaaaata taagaaatga aataataagt ttaggtggtc aagttagatt taattcaaaa
    33001 gtaacagata tagttgtatc aaatgattct atacaatctg taataataaa tgatactgaa
    33061 aaaatagata cagatacaat tatattagct gttggacata gtgcaagaga tacttatgaa
    33121 atgttgtatt caagaggtat aaaaatagtt caaaaacctt ttgcaatagg agctagagtt
    33181 gaacatccac aagatttaat aaataaagca cagtacaagg atttttataa tcatcctagg
    33241 ttaggagctg cagattatag acttatagag catgtttcaa atggaagaac agcatatact
    33301 ttttgtatgt gtccaggagg tagtgtcata gcatctgcat caaatgattt tgaaatagtt
    33361 acaaatggta tgagtgaaca tgcaagagat aaaataaatg caaatagtgc atttttagta
    33421 aatgtaacgc cagaagattt taagagtgat aattcactag caggtgttca tttccagcag
    33481 aagtatgaaa aattagcttt tgaattaggt ggaaaaaatt ataatgcacc agttcagctt
    33541 gttggagatt ttataaacaa taagacttct ttgaatttag gaagtgttga gccatcatat
    33601 agaccaggat atacacttac aaatttaagt gaatgtttgc ctagcttcgt aacagagact
    33661 atgagagaag cgtttatatc tcttgataaa aaattgaatg ggtttgcaat gtatgatgct
    33721 gttttgacgg gagtagaaac taggtcatca tcaccaataa gaatagtaag agatgaaaat
    33781 actttagaat cagtgagtat taagaatcta tatccatgtg gtgaaggtgc tggatatgca
    33841 ggtggtatag ttacagcagc agttgatggt ataaaatgtg cagaaaaaat aatacaaaaa
    33901 tacaccaata atacaaaaat ttaacataaa cttaatgatt ttttaatatt taacatgaat
    33961 ttaacattat cactcctata atttgataaa atacaatagg ttataaaatt tttagataca
    34021 gtaatctctt tacaaggagg aaataatttg aacattataa taagtctaat gggtggatta
    34081 ggtctgttct tatatggtat gaaccttatg ggagaaggtc ttcaaaaatc tgctgggaca
    34141 aagctaaaaa agataataaa actacttact agtaacctat ttatgggagt tttggtaggt
    34201 acaggagtta cagcagtaat acaaagttca agtgctacta ctgtaatggt tgttggtttc
    34261 gtaaatgcag gaataatgac tttaaaacag gcaataggag ttataatggg tgctaatata
    34321 gggactactg ttacagccca attggtatct ttcgacttaa ctggtatggc cccagtagca
    34381 ttaggtgtag gtattatact ttatttattt ggaaataagc ctagaataaa aaatatagcc
    34441 gaaatactta taggttttgg tatattattt acaggtatgg attttatgaa gatggcagtg
    34501 gagccactaa gagactatca aggatttact gacttacttg ttacatttgg tagatatcca
    34561 ctacttggat tattattggg atttgggatt acagcaataa tacaaagttc aagtgcatct
    34621 atgggtatgt tggttgcact ggcagcagaa ggtctagtgc cattatcagc agcactgcca
    34681 attctttatg gccaaaatat tggtacatgt gtaacttcac tgttatcaag tattggtgct
    34741 aacaaaaatg cacgaagagc agctatgatg catctaatct tcaatgtttt aggaacagtg
    34801 atattcttaa tattcttgaa taaaccagta gtaagcatgg ttacaagttg ggacccaagc
    34861 aatgttgcaa gacaaatagc caatacacat acattgttta atataatatc tgtattgata
    34921 cttttaccat ttacaaatct tattataaaa ctagctataa agcttgttcc tgatagagca
    34981 ggtgatatag atgaagatga aactaaaact attaaatata tagatgatag aatgatagaa
    35041 actccttcta tagctcttgc aaatacgata aaagaagcac ttcgtatggg tgagaaggcg
    35101 aaggaaagcc taaatgcatc tatggaagct ttagtagaac attcaactga aaaaatagat
    35161 aagacatata ggcgagagag attaatcaat gatttacaga aagctatttt aaattattta
    35221 cttaaacttt caaaagcacc tttagatgat gattcaagag aagttgtgga tactttgttt
    35281 aatacagtca atgatataga aagaattggt gaccacgcta aaaatatagc tgaattatcg
    35341 caagttgcaa tagattcaaa tatttctttc tcagaagaag gacaatctga acttgatgtt
    35401 atgtataata gagttgtttc agcatatact tatgcattag aatcaatgag aactgataat
    35461 gttgatttag cttgtaaggt tataaaaatt gaagaacaag ttgatataat ggagaaatct
    35521 tgtagagcta atcacatgta tagattgaac aataatctat gtagtataga aaatggagtt
    35581 atttatttag acgtaataag taatttagag agaatatctg accatgctgt caatatagca
    35641 caacaagtta tagcaaagag attaggaaat gattaaaaat aattaattta aaataatata
    35701 aaatttaaaa tagaggataa ataaaagaga aactttaaat gatacgttta gagtttcttt
    35761 ttttgtaaat ttatttgact aaaattttta aatttataca tagttatagc atttagaaga
    35821 atcgatttcc tgataataaa tgatgagata tgtatgattt tgtggcaaac ttttaaaaaa
    35881 aacacctata ttttgtgtta taatctcttt aaaacgaata tgctgaaaag agggatttaa
    35941 atgggaaaaa taaattttga ttatagtaaa gctacagagt ttttttgtca aaacgaaata
    36001 gatgtcatgc aaccatatgt agatgttgcg cacgacatgt tacataataa aactggttta
    36061 ggaaatactt ttttaggatg gatagacctt cctaaaaatt atgataaaga agagtttgac
    36121 agaataaaaa aatctgctga gaagataaaa tcagactcag atgttctttt ggtaataggt
    36181 ataggaggct cttatttagg ttcgagagct gctatagata tggtaagtca ttcttttagg
    36241 aatggactta aaaaagagca aagaaaggct ccagaagtat attttgtagg gcataatata
    36301 agctcaacat atattatgga tttattagat ataattgaag gaaaggatat ttctgtaaat
    36361 gtaatatcta aatcaggaac tactacagaa ccagctctgg cttttagaat atttaaagat
    36421 tacttagaga aaaagtatgg taaggaagaa gcaagaaaga gaatttatgc aacaacagat
    36481 gcaagtaaag gtgcacttag acagttagct acagaagaag gatatgagac ttttgtaata
    36541 ccagatgatg taggtggtcg tttttctgta ttgacagcag ttgggttact tcctattgct
    36601 gctgctggac ttgatattga tgctatgatg aaaggcgcaa atgatgctag agaagcattt
    36661 caaaatcctg acttaaaaag taatgatagt tatagatatg ctgttgctag aacaatttta
    36721 catagaaaag gaaaagatgt agagttatta gtgaactatg aacctcaact acattatgta
    36781 agtgagtggt ggaaacagct ttatggagaa agtgaaggaa aagaaaataa aggtttattc
    36841 cctgcttctg tagatttctc aacagattta cattctatgg gtcaatacat acaagatgga
    36901 aaaaggcttt tatttgagac agtacttaat gttgaaaact gtaagagaaa tataactata
    36961 agttcagagg aagtagactt agatggttta aactatttag caggtaagac tgtagatttt
    37021 gtaaatcata aagcttttga aggaactcta ttggctcata cagatggaaa agtacctaat
    37081 ttagtaataa atatacctca actagatgag tataattttg gatatttagt ttatttcttt
    37141 gagaaggctt gtggaataag tggatattta ttgggagtta atccatttga ccaaccagga
    37201 gtagaagctt acaagaaaaa tatgtttgct ttactaggca aaccaggtta tgaaaaagaa
    37261 aaggaagagc tagaaaaaag acttaaataa ttttaaatag ttaaggttta attaagatta
    37321 aaataatata atgaaatcat caaaaaggag tttactcaaa atagatgatt tcattttttt
    37381 tatgaaaaat tgggaaaaat atattttgat aatttatatt atattaagat agggagtggt
    37441 atgagatgtc aagaagaaag aaagggataa gtttagtaat attagttgct ataataagtt
    37501 caatacttag tagttttcta acaataattt tagttaagga caatttagta agcaaatcta
    37561 caggaagttc tacgccgata gttgtaaatg atgatggtaa gagtcaaaat atttatcaag
    37621 cagttgcaga aaaagctacg ccatctgttg taggtattac aacaactagt gttgatacta
    37681 gtaatatgtt tgcaatacca actgaaacac aaggtgtagg aactggaatc atagttgatt
    37741 caaatggata tatacttact aattctcatg ttatatcaga tggtcaagct acgagtgtaa
    37801 atgtattatt caatgatggt tcaacaactt caggtaaagt agtatggttt gaccaacaac
    37861 ttgatttagc tatagttaag gttgataaaa ctggactaac accagcagaa tttgctgata
    37921 gtgacaaagt aaaagttgga gatatatcaa tagcaatagg taatcctctt ggattagatt
    37981 ttcaaaaaac agttactcaa gggataataa gtggattaga tagaactata caaacagaaa
    38041 aaactaatat gactggttta ttacaaactg atgcaagtat aaatgcagga aatagtggag
    38101 gaccattgtt aaatcaaaaa ggtcaagtaa ttggtataaa cacagcaaaa gcttctcaag
    38161 cagaaggatt aggatttgca ataccaatca atacagctaa gagtatagtt gaagaagtaa
    38221 tcaaaaatgg aaaatatgaa aaagtaactt taggcataaa aggtacagat gtatcaaatt
    38281 atgaagcagc aacaggtaca aaattaagta ctgataaagg tgtttatgtt gcagaagtaa
    38341 tatctggttc atcagcagaa aaagctggag taaaggtagg ggatattata actaaagtag
    38401 gagatacaga tataacaggt atgaatgatt taaataaaaa gttgtatact ttctcaaaag
    38461 gtgctagtac aaaaataaca gtaaatagag gcggaaaagc agtaacaata aatgtgaatt
    38521 tctaaatatg accagaaaga tagagactac atggtaaagt gtagtctcta tttttattta
    38581 atttttatag tatatacatc taaattaatt aaaatgatgt attgtttata gcttattgct
    38641 atattttctt ataaaaataa atttaataaa tatttaaatt taatttacaa aaacaatata
    38701 aatatcataa taaaataaaa ttttttaata ttactaaaaa tatatagaca aaataataac
    38761 aaatatagta ttatgtaatt gtagaaatgc ttttcatagg tatattctat gaagatgaat
    38821 tgcataaaaa tgaaaaaatg gaatggatat tatttagggg tgaaaaatca tgaatccatt
    38881 agttataaac ttacaaaaat gtagcataca tgatggacca gggataagaa gtacagtatt
    38941 ttttaaaggg tgtccacttg aatgtgtttg gtgtcataat ccagagagtc aaacatatac
    39001 aaaacaagtt ttgtataatg aagaaagatg ttcaaaatgt gaagcatgta taaatatatg
    39061 ccctcataag gcaatttaca aaggtgaaac aaaaatatgc ttagaccaag ataagtgtga
    39121 gttttgtgaa acttgcttag actattgtgt aaacaatgca agagagattg ttggtcaaga
    39181 gtattctgta agagatttgg ttaaagagat agagaaggat agaattttct atgaagaatc
    39241 aggtggtgga gtaactctat caggtggaga agtaatggct caagatatgg acttcatttg
    39301 tggagtaata aacatgtgca aatcaaaggg aatccatgta gcaattgata catgtggcta
    39361 tgcaaagtca gaaaattatg aaagagttgc taaatgtgca gatttgtttt tatatgatat
    39421 taagcttatt gatgaagata agcacataaa atttacagga aaatcaaatg acttaatact
    39481 gaaaaatgtt aagattttaa gtgaattagg ggctaacata aatataagaa taccattaat
    39541 tgtaggagta aatgtagatg atgaaaattt agaggttaaa aaaatgatag agtttttaaa
    39601 gcctttgaat atacaagcag taagtcttct tccatatcat aatattggta aacacaagta
    39661 tgataaaatt tataaaaagt atgaaggaga agaactacaa agaccatcag aagaaaaatt
    39721 agaggaaata aaaagactgt ttgaagcaag taatttcaat actaagattg gagggtaata
    39781 aaatggcaag aggaactttt gagagaacta aaaaattaag agaagaaagt attaatgcag
    39841 aaccacacat atctattgaa agagcagtac ttatgacaga agcttataaa aaatatgagg
    39901 gaagtgtaga gattcctgta cttcgtgctt tatcatttaa gcattatatt gagaatagaa
    39961 ctcttagcat aaatgatgga gaattaatag taggtgaaaa aggtgattct cctaatggag
    40021 caccaacata tccagaaatt tgttgtcata caatggaaga tttagaagta atgcacaata
    40081 gagatataat aaattttagc gtatcagaag aagctagaaa aatacataaa gaagaaataa
    40141 ttcctttttg gaaaaagaga caaacaagag ataagataat aaatgcaatg acacctgagt
    40201 ggttagcagc atatgaagca ggtatgttta cagagtttat ggaacaaaga gccccaggac
    40261 atacagtttg tggagataca atatataaaa aaggattttt agatttaaag aaagacatag
    40321 aagcaagatt aaaagagcta gattttctta atgatttaga tgcatataat aaaaaagcag
    40381 atttagaagc tatggcaata gcttgtgatg caatggttat attaggtaaa agatatgctg
    40441 agaaagcaag acaaatggca gaagaagaaa cagatgaagc taagaaaaaa gatttattac
    40501 taattgctga aacttgtgat gttgtaccag ctcacaaacc agaaacttat catcaagcta
    40561 tacaaatgta ttggtttgtt catatagggg ttactactga acttaatata tgggatgctt
    40621 ttactccagg aagacttgac caacatctaa atcctttcta tgaaagagat gtagaaaatg
    40681 gaatactaga tagagataga gcacaagaat tacttgaatg tttatgggtt aaatttaaca
    40741 atcagccagc accaccaaaa gttggtataa cattaaaaga aagtagcaca tatacagatt
    40801 ttgcaaatat aaacactggt ggaataaatc cagatggtca agatggtgtt aatgaagtta
    40861 gttatataat acttgatgtt atggatgaaa tgaaattgat acaacctagt tcaaatgttc
    40921 aaataagtaa aaagactcct caaaaattct taaagagagc ttgtgaaata tcaagaaaag
    40981 gatggggtca accagcattt tataatactg aagcaatagt tcaagagctt atggaagctg
    41041 gaaaaactat agaagatgca agacttggtg gaaccagtgg ttgtgttgaa actggatgtt
    41101 ttggtaaaga agcatatgtt ctaactggat atatgaacat tccaaaaata cttgaattaa
    41161 cattaaataa tggatatgac ccaattagta aaaaacaaat aggtattgaa actggagacc
    41221 ctagaaattt ccaatcttat gaggaactat ttgaagcatt taaaaagcaa ttacattata
    41281 tgattgacat aaaaatagaa ggaaatgctg tgatagaaaa tatatgtgca aaacacatgc
    41341 cttgtccact tatgtctaca atagttgatg attgtataga aaaaggcaag gattaccaaa
    41401 gaggtggagc aagatacaac actagatata tacaaggtgt tggtatagga actataacag
    41461 atagtttaac agcaataaaa tataatgtat ttgataagaa aaaattcgat atggatacat
    41521 tgcttaaagc acttgatgct aattttgaag gatatgaagc tatacttaat ttagtttcaa
    41581 ataagactcc taaatatgga aatgatgatg attatgctga tgaaataatg caagagatat
    41641 tcaatgcata ttacaatgag gtaactggta gaccaactgt ttgtggtgga gaatacagag
    41701 tagatatgtt accaacaact tgtcatatat actttggaga aattatggga gcaagtccaa
    41761 atggtagact ttgtgcaaaa ccagtttcag agggaatttc acctgaaaaa ggtggagata
    41821 ctaatggacc tacagcagtt ataaaatctt gtgctaagat ggaccacata aaaactggtg
    41881 gaacattatt aaatcaaaga tttgcaccat cagtagttca aggagaaaaa gggttagata
    41941 atatggctaa tctagtaaga gcatacttta atatggatgg tcatcatata caatttaatg
    42001 tatttgataa aaatgtactg ttagaggctc aaaagaatcc tcaggattat aaagacttaa
    42061 tagttagagt tgcaggatat agtgaccatt tcaataattt aagtagaaca ctacaagatg
    42121 agataatagg aagaactgag caaacattct aggtttgctt gtaaataaaa cttcttacta
    42181 tataaaatcg taactgatat aataaagtat aaatggtttg attccatttt taaaatttta
    42241 gattaattat gagctaactt tctaaatagg aagttagctc gttaagttta atgaaaaaat
    42301 cgttaaattt atatttagta aatcatacac atattagtaa gtaatacata caaaagaata
    42361 atttattata gttataataa ggttatattt tataaaaata agataatttt aagcagttaa
    42421 aataaagtca atttagctac tttaaaataa aactaataga aaaaaggaga tttgatatga
    42481 aaactttagg atttattggt tcagggaata tgggaagtgc aatgataggt ggcatagtac
    42541 agtcagaatt agtaaaggca gaagatataa ctgtatcaga tttaagccaa gttgccttag
    42601 ataaagtaaa agaagaattt ggagtaaata ctacaactga ttcaaaggta gctgttacaa
    42661 attctgatgt ggtaatagta gcacttaaac ctaatatatg cgaaaaggct ttaactccac
    42721 ttaaagaatt aatagatgca aataaaataa tagtatctat agcagcaggt aaaactatag
    42781 aaagtctgga aagttttata ggttcagata aaaaaatagt aagagtaatg cctaatactc
    42841 cagcccttgt tggcgaagga atgtcagctc tttgtaaaaa tagtaatgta acagatgaag
    42901 agttaaatat gattaaggct ctgtttgaat cttttggtga ggcagaaata gtatcagaat
    42961 atttaatgga tacagttaca ggtattagtg gttcttctcc tgcatatgta tttatgttta
    43021 tagaagctat ggcagatgca gcagtattag ctggaatgcc aagacaacaa gcttataaat
    43081 ttgcatctca agcagtaatg gggtcagcta agatggttct tgaaactgga aaacatccag
    43141 gtgaattaaa agatatggtt tgttctccag caggaactac tatagaagct gttaaggtat
    43201 tagaagaaga aggctttaga gcagcagtta taaaatctat tgttgcttgt atagataagt
    43261 ctaaagatat gagcaaataa cagatgtaat taagtttact atatggtttc ttgaattgtt
    43321 tttttagtgc ttatttatat ctatacataa atgtatttgg tataagtaaa ataagagtaa
    43381 tttactataa aaaggaattt aatgattgag tgagaattta ttacttaaat attaataatt
    43441 aaatagatat atttttaact atatttgaga tatgtcattg tgaaaaaata aaatttataa
    43501 gtattattgt gaggttataa gtagatttat ttaaaaataa atttatttat agccttttta
    43561 aatttgaaat aatattcata aggtaataaa agttacaaat attttcttcg ctaataagtt
    43621 ttaaaatacc tataatgttt tact
//
LOCUS       DAELNY010000014        43501 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.014, whole genome shotgun sequence.
ACCESSION   DAELNY010000014 DAELNY010000000
VERSION     DAELNY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.014"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(121..894)
                     /locus_tag="KPE55_000642"
     CDS             complement(121..894)
                     /locus_tag="KPE55_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG0491743.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KPE55_000643"
     CDS             complement(894..1280)
                     /locus_tag="KPE55_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491744.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KPE55_000644"
     CDS             complement(1305..1622)
                     /locus_tag="KPE55_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG0491745.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KPE55_000645"
     CDS             complement(1634..1894)
                     /locus_tag="KPE55_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG0491746.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KPE55_000646"
     CDS             complement(1894..2190)
                     /locus_tag="KPE55_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0491747.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPE55_000647"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPE55_000647"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG0491748.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPE55_000648"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPE55_000648"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG0491749.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPE55_000649"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPE55_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG0491750.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPE55_000650"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPE55_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG0491751.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="KPE55_000651"
     CDS             complement(8083..9189)
                     /locus_tag="KPE55_000651"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG0491752.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPE55_000652"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPE55_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG0491753.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="KPE55_000653"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="KPE55_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG0491754.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="KPE55_000654"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="KPE55_000654"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG0491755.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="KPE55_000655"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="KPE55_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG0491756.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="KPE55_000656"
     CDS             12283..12990
                     /locus_tag="KPE55_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0491757.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="KPE55_000657"
     CDS             12959..13588
                     /locus_tag="KPE55_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG0491758.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="KPE55_000658"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="KPE55_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG0491759.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="KPE55_000659"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="KPE55_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG0491760.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="KPE55_000660"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="KPE55_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG0491761.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="KPE55_000661"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="KPE55_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG0491762.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="KPE55_000662"
     CDS             18864..19637
                     /locus_tag="KPE55_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG0491763.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="KPE55_000663"
     CDS             19639..20502
                     /locus_tag="KPE55_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG0491764.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="KPE55_000664"
     CDS             20535..21677
                     /locus_tag="KPE55_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0491765.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="KPE55_000665"
     CDS             complement(21810..22385)
                     /locus_tag="KPE55_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG0491766.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKLTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="KPE55_000666"
     CDS             22645..23937
                     /locus_tag="KPE55_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0491767.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="KPE55_000667"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="KPE55_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG0491768.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="KPE55_000668"
     CDS             24717..24980
                     /locus_tag="KPE55_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG0491769.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="KPE55_000669"
     CDS             24995..25180
                     /locus_tag="KPE55_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG0491770.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="KPE55_000670"
     CDS             25283..26479
                     /locus_tag="KPE55_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0491771.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="KPE55_000671"
     CDS             26693..26971
                     /locus_tag="KPE55_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG0491772.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="KPE55_000672"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="KPE55_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0491773.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="KPE55_000673"
     CDS             27461..27688
                     /locus_tag="KPE55_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG0491774.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="KPE55_000674"
     CDS             27831..28151
                     /locus_tag="KPE55_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0491775.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="KPE55_000675"
     CDS             28159..29115
                     /locus_tag="KPE55_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG0491776.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="KPE55_000676"
     CDS             29126..31120
                     /locus_tag="KPE55_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG0491777.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="KPE55_000677"
     CDS             31121..31570
                     /locus_tag="KPE55_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG0491778.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="KPE55_000678"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="KPE55_000678"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG0491779.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="KPE55_000679"
     CDS             33122..33430
                     /locus_tag="KPE55_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491780.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="KPE55_000680"
     CDS             33512..34801
                     /locus_tag="KPE55_000680"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG0491781.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="KPE55_000681"
     tRNA            35127..35202
                     /locus_tag="KPE55_000681"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="KPE55_000682"
     tRNA            35209..35283
                     /locus_tag="KPE55_000682"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="KPE55_000683"
     tRNA            35288..35363
                     /locus_tag="KPE55_000683"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="KPE55_000684"
     tRNA            35397..35471
                     /locus_tag="KPE55_000684"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="KPE55_000685"
     CDS             complement(35854..36846)
                     /locus_tag="KPE55_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0491782.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="KPE55_000686"
     CDS             complement(36855..37913)
                     /locus_tag="KPE55_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0491783.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="KPE55_000687"
     CDS             38038..39501
                     /locus_tag="KPE55_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG0491784.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNLDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFNVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="KPE55_000688"
     CDS             39603..40400
                     /locus_tag="KPE55_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0491785.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="KPE55_000689"
     CDS             40413..42515
                     /locus_tag="KPE55_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG0491786.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="KPE55_000690"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="KPE55_000690"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG0491787.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43129..>43501
                     /locus_tag="KPE55_000691"
     CDS             43129..>43501
                     /locus_tag="KPE55_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491788.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKYSIKNVVSISSILMQNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQI
                     KFPLIYIDEDSFCPYCLGNKFN"
BASE COUNT        16378 a         5500 c         6896 g        14727 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtgcatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tgggttttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca acgagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttccta
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataaaat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ctctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagctcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttca
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggtcc ctaagaacct
    21781 ctaatctatt tgataattgt tttaagaagt tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 agtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagaataa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa acttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtattcat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttacg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattgaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaaccg ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagagtct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaaca taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagaacaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt ataggaccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttta cttacattga ttttttatta agttaaagct
    34921 acttatacat attctagctg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 atagtattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taagatacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag actctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aagtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcgcttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacctt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tctccattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag agacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacttaga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagcgga aaacgttaga gattttgcat ttatactaag tagcaaattt
    38821 aatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagcga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcctt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aacagtgaat gtaagtaaat tttgtgtaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aagaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttga
    40261 gtaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aacggctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtgataatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac catcacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt caagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gaacttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctctagggct ttctgatgag gttttaggaa gggctgatta taggttatct
    42961 ttttctagga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gttttttatg
    43081 atataattga attataaaat aaaatcaaaa tgtaaagtag gtgtagtaat ggacagacta
    43141 gatatattta tcgaaaatta tgattttaaa gaatatcttg aaaaattacc tagagaatta
    43201 atagaagaaa attataaaga ttttgttata gaattttgga aaaatcctag tgtagatatt
    43261 attaaactaa aatcaaaata tagtataaaa aatgtagtat ctataagtag tatattaatg
    43321 caaaatccta tccaagagta ttatgagttt cttgaagata tttggaaaaa taacataaaa
    43381 atagatgaat taattaataa gtatttaatt acagcaacaa gaatggatca aattaaattc
    43441 ccattaatat atattgatga agattctttt tgtccttatt gtttaggtaa taaattcaat
    43501 a
//
LOCUS       DAELNY010000015        43141 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.015, whole genome shotgun sequence.
ACCESSION   DAELNY010000015 DAELNY010000000
VERSION     DAELNY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.015"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..527
                     /locus_tag="KPE55_000692"
     CDS             75..527
                     /locus_tag="KPE55_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0491789.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            530..838
                     /locus_tag="KPE55_000693"
     CDS             530..838
                     /locus_tag="KPE55_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0491790.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            952..2058
                     /locus_tag="KPE55_000694"
     CDS             952..2058
                     /locus_tag="KPE55_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0491791.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2133..4811
                     /gene="mngB"
                     /locus_tag="KPE55_000695"
     CDS             2133..4811
                     /gene="mngB"
                     /locus_tag="KPE55_000695"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0491792.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5128..7461)
                     /locus_tag="KPE55_000696"
     CDS             complement(5128..7461)
                     /locus_tag="KPE55_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0491793.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALDIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(7436..8152)
                     /locus_tag="KPE55_000697"
     CDS             complement(7436..8152)
                     /locus_tag="KPE55_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491794.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(8566..9222)
                     /locus_tag="KPE55_000698"
     CDS             complement(8566..9222)
                     /locus_tag="KPE55_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0491795.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(9277..9906)
                     /locus_tag="KPE55_000699"
     CDS             complement(9277..9906)
                     /locus_tag="KPE55_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0491796.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQVGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(10037..10678)
                     /locus_tag="KPE55_000700"
     CDS             complement(10037..10678)
                     /locus_tag="KPE55_000700"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG0491797.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(10687..11358)
                     /locus_tag="KPE55_000701"
     CDS             complement(10687..11358)
                     /locus_tag="KPE55_000701"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0491798.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(11363..12247)
                     /gene="rsgA"
                     /locus_tag="KPE55_000702"
     CDS             complement(11363..12247)
                     /gene="rsgA"
                     /locus_tag="KPE55_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0491799.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(12345..13082)
                     /locus_tag="KPE55_000703"
     CDS             complement(12345..13082)
                     /locus_tag="KPE55_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0491800.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(13190..15205)
                     /gene="pknB"
                     /locus_tag="KPE55_000704"
     CDS             complement(13190..15205)
                     /gene="pknB"
                     /locus_tag="KPE55_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG0491801.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(15222..15974)
                     /locus_tag="KPE55_000705"
     CDS             complement(15222..15974)
                     /locus_tag="KPE55_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG0491802.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(15994..17025)
                     /gene="rlmN"
                     /locus_tag="KPE55_000706"
     CDS             complement(15994..17025)
                     /gene="rlmN"
                     /locus_tag="KPE55_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG0491803.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(17033..18358)
                     /gene="rsmB"
                     /locus_tag="KPE55_000707"
     CDS             complement(17033..18358)
                     /gene="rsmB"
                     /locus_tag="KPE55_000707"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG0491804.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(18420..19121)
                     /locus_tag="KPE55_000708"
     CDS             complement(18420..19121)
                     /locus_tag="KPE55_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG0491805.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(19190..19942)
                     /locus_tag="KPE55_000709"
     CDS             complement(19190..19942)
                     /locus_tag="KPE55_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG0491806.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(19952..20881)
                     /locus_tag="KPE55_000710"
     CDS             complement(19952..20881)
                     /locus_tag="KPE55_000710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG0491807.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(20896..21336)
                     /gene="def"
                     /locus_tag="KPE55_000711"
     CDS             complement(20896..21336)
                     /gene="def"
                     /locus_tag="KPE55_000711"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0491808.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(21354..23879)
                     /gene="priA"
                     /locus_tag="KPE55_000712"
     CDS             complement(21354..23879)
                     /gene="priA"
                     /locus_tag="KPE55_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG0491809.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(24118..25317)
                     /gene="coaBC"
                     /locus_tag="KPE55_000713"
     CDS             complement(24118..25317)
                     /gene="coaBC"
                     /locus_tag="KPE55_000713"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG0491810.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(25322..25588)
                     /locus_tag="KPE55_000714"
     CDS             complement(25322..25588)
                     /locus_tag="KPE55_000714"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG0491811.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(25591..26208)
                     /gene="gmk"
                     /locus_tag="KPE55_000715"
     CDS             complement(25591..26208)
                     /gene="gmk"
                     /locus_tag="KPE55_000715"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG0491812.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(26238..27119)
                     /locus_tag="KPE55_000716"
     CDS             complement(26238..27119)
                     /locus_tag="KPE55_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG0491813.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(27123..27953)
                     /locus_tag="KPE55_000717"
     CDS             complement(27123..27953)
                     /locus_tag="KPE55_000717"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG0491814.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(28240..29274)
                     /gene="ltaE"
                     /locus_tag="KPE55_000718"
     CDS             complement(28240..29274)
                     /gene="ltaE"
                     /locus_tag="KPE55_000718"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG0491815.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            29570..31345
                     /locus_tag="KPE55_000719"
     CDS             29570..31345
                     /locus_tag="KPE55_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG0491816.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(31560..33212)
                     /gene="abc-f"
                     /locus_tag="KPE55_000720"
     CDS             complement(31560..33212)
                     /gene="abc-f"
                     /locus_tag="KPE55_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBG0491817.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(33511..34722)
                     /locus_tag="KPE55_000721"
     CDS             complement(33511..34722)
                     /locus_tag="KPE55_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG0491818.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIASITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(35006..35539)
                     /gene="pyrR"
                     /locus_tag="KPE55_000722"
     CDS             complement(35006..35539)
                     /gene="pyrR"
                     /locus_tag="KPE55_000722"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG0491819.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(35899..36846)
                     /locus_tag="KPE55_000723"
     CDS             complement(35899..36846)
                     /locus_tag="KPE55_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0491820.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(36815..37261)
                     /gene="lspA"
                     /locus_tag="KPE55_000724"
     CDS             complement(36815..37261)
                     /gene="lspA"
                     /locus_tag="KPE55_000724"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0491821.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(37571..38350)
                     /locus_tag="KPE55_000725"
     CDS             complement(37571..38350)
                     /locus_tag="KPE55_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0491822.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(38405..38887)
                     /locus_tag="KPE55_000726"
     CDS             complement(38405..38887)
                     /locus_tag="KPE55_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0491823.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(39153..40574)
                     /locus_tag="KPE55_000727"
     CDS             complement(39153..40574)
                     /locus_tag="KPE55_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0491824.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(40720..41490)
                     /locus_tag="KPE55_000728"
     CDS             complement(40720..41490)
                     /locus_tag="KPE55_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0491825.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(41495..42697)
                     /locus_tag="KPE55_000729"
     CDS             complement(41495..42697)
                     /locus_tag="KPE55_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0491826.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        14201 a         7547 c         4645 g        16748 t
ORIGIN      
        1 atatttaatt taattaggat aatgattaca atataaatat aagttaagac attatgaatg
       61 gagtggaaaa atatatggta ctacaagaaa aatatatatt tatagataaa gacttaaaaa
      121 gcaaagaaga agtattaaat tttattgcag atagtgctta tatactaggt attacaacta
      181 ataaagaggc tcttttaaaa gaccttctaa aaagagaaaa tgaatttcca acaggaataa
      241 aagatggatt tgcgatacct catacaaaga gtgtaaatgt aaaggaagct tccatactct
      301 atattaaaac aactaacgag ttagagtgga aaactttaga tgatagtaag gttagatatg
      361 tatttagttt gttagcccca cataaaaatg aaggaaatat acatttgaaa actctatcta
      421 aattagccac atgtttaatg gaggatgatt ttacaagtga agttaaaaat gctagtgatg
      481 caagtcattt agtaaaatat ataactaata aaatgaagga ggaataatta tgaaaatagt
      541 agctataaca tcatgtatag caggattagc acacacacct atggcagcaa aagctttaga
      601 acaagctgca tcaaaaatgg gtcatgaaat caaagtagaa caacaaggtg ctatgggacg
      661 agtaaatact ataacaaaag aagaagcaca agatgcggac tttatactta tagcatctga
      721 ccaaactata tctggaatgg aaagatttga tggtaaaaaa gtgataaaag tcaaaatagg
      781 tatagcgtta aaaaaaccag aagctgtctt aacaaagtgt atagaagcta tttcttaata
      841 aaaaaatctc actctaatat agaatgagaa aaatcaaaaa ttaaaaaatt gtctaatgta
      901 attataacat attatcaaaa aatataacaa acttatatag gaggattaaa aatgagtaaa
      961 aaaaataaat ctcttttaag tgaacttaag ggacacttaa tgacaggtat ctcatatatg
     1021 ttgccacttg taataggctc atctttagtt gtagctatcc caaagcttac agctttagca
     1081 gtgggtataa caagtttaga tgcttataaa aatgtatctg gtttttatca tattctttac
     1141 ttaatggaac aagttggttg gactggcata ggtctattaa atacggtatt agcaggattt
     1201 atagcatttt caataggtga gaaaccagct atgggtgcag gttttattgg tgggctcata
     1261 gcaagtaata ctaatgcagg attcttagga gcagtaatag caggtttctt tgctgggtat
     1321 gtttgtaagt ttttaaagaa aaaaattaaa atatctgggt cagcatcagg aatgatgcca
     1381 cttataataa tgccacttat aacagttgga ttaactggtt ttttaatgag tgttgtatta
     1441 gcaggacctt taggaaacat aaatacttca ttaaatgctt gggtagctga aatgtgtcaa
     1501 aatggaacaa attcggtggt attggcttta attttaggcg caatgatagg attcgattta
     1561 ggtggaccag taaataaagc agcttggatg gcaggaaatg cattattgtt agagggaatc
     1621 tatctaccag cgatattggt aaactgcgca atagttatac ctccactagg atatggttta
     1681 gctacattca taagaaaatc aaaattttcc aatgcattag ctgaaacagg aaaaggcaat
     1741 atagttatgg gtataattgg aataacagaa ggtgcaattc ctttcacttt aacaaatcca
     1801 ttaaaattag ttcctgttaa tatgatagga tgcgctttgg gtgtaggatt atcagctttg
     1861 ttaggtgtac atgctataat gcctccagtt ggtggattat atggatttat atcaataggt
     1921 tctggatggg cttacttagt aggtgcaata tttggagctt tagtaatagc aatattatca
     1981 acaactttag tagacttcaa tgaagatgaa actacggaaa atacagaatt tgatgaaata
     2041 gagttagata taatgtaaat taaaaactta attttagata attgagaatt gagtgtactc
     2101 tcaattctca attaaatttt gggaggattt ttatgaaaaa gacaaaagta catgtagttc
     2161 cacacagtca ttgggataga gaatggtatt ttacaacatc aaggtcaaaa atatatttaa
     2221 tgaaagattt tcaagatata ttagacatat tggaagaaaa agaagacttt aaatacttta
     2281 ctatggatgc acaagcttct ctcttagatg attatttaaa atggagacct caggataaat
     2341 atagaataag agatttagtt caaagaagaa gattagttat tggaccttgg tatactcaaa
     2401 cagaccaatt ggttatatca ggagaatcta taattagaaa cctttactat ggaatagata
     2461 gatgcaatga atttggtgaa cctatgaggg ttggatatgt accagattct tttgggcaat
     2521 cagctcaaat gccacaaata tacagaggtt ttggaataga tagttcatta ttttggcgtg
     2581 gagtttctga tgatatggta gatacaacag agtttatatg gaaaggttct gatggttcaa
     2641 aagtattagc agttcaaata ccatttggtt attactatgg aggtaatata ccagaagatg
     2701 attcagattt aaaagaatat ttaaaagata taatcgtaaa acttaaggtt aaagcttcta
     2761 ctaataacgt atattttcca aatggatttg accaagcacc aataagaaaa aaccttcctg
     2821 atatattaaa aaaagctaat gaaatagata atgaaaatga atacgaaata agtgatatag
     2881 aaaactatat agattcagta agaaatgaaa gaaatgaatt tacagagctt aaaggtgaat
     2941 ttttaaatgc taaacatatg agaatacaca aatccatatt ctcctcaagg tcagacctta
     3001 aaataatgaa taacaaatta gaaaattata ttacaaatgt acttgaaccg ttattaacat
     3061 taagttattc acttggaaat gaataccctc acctagttat aaaagatata tggaaactta
     3121 tgtttgaaaa tgcagctcat gacagcatag gttcttgtaa tagtgacaca acaaatgagg
     3181 atgtatacac aagatacaaa caagccagag atttgagtat aaatttatta gatttgcata
     3241 tgagacttat aagtactcaa atcaaaaata ataatgaaga aataacatta acagtattca
     3301 atggattccc agaagtcaga aatgaagttg tagaatttga tacttatatt ccaggtcaag
     3361 attttgtgat aaagaataaa aatggaaaaa ttcttaatta tataataaaa gaaaaagaag
     3421 atattacaaa ttatatatta actcaaacaa taagattaaa cccaagtaaa aaaatatata
     3481 tacccaaaac tgtatataga gcaaaaattg cactttcagt aaaagatata ccaccaatgg
     3541 gatatactca actagttttt gaattggata ctaaaaataa atgtgaaata gaaacaagta
     3601 attgcaagga aatagaaaac aaatactaca aaataacagc caataaaaat ggttcattag
     3661 aaatattaga taaagaatct aataagactt ataaaaacca agctgtaatt gaagacaatg
     3721 gagatgatgg agattcatac aactattctc ctccaagaaa agatatttat gtttcatctt
     3781 tagattctgt atcttcagta gaagtgctaa aatcaaacat tcaagaagaa atgatactta
     3841 aatattcatt gaccatacca gcttcactgg aagaaagggc tataggtaaa gtttctgtga
     3901 aaatgccagt aacaatgaaa atcaccttag aacctaaaga acaaataatt aagtttaatg
     3961 ttaatataaa aaataaccaa gcactatctc atagagtaag agttttattt aatacagaaa
     4021 tagcatctaa gttttctata gcagatcaac aatttggaat aatacaaaga ccaacagtat
     4081 tagaaaaaga ccttgcattg tggaaaagag acaattactc atggcaagaa aaacctataa
     4141 ctatcgaacc aatgcaaagt tttgttactt tagaggatgg acaaagagga atagcattaa
     4201 taactggttg tgtaagagaa tatcaaatag ttggtgataa tttagataca atagcattaa
     4261 cactattcag gtcatttgga tatatgggaa gagaaaattt actttataga ccaggaagag
     4321 cttctggaga aaaaataata gcaacaccag atgcacaatt acttaaagag ttaaactttg
     4381 actttggtct ttacatatat aacaataaat ttgatgaagc aaatgtagct aatacagcta
     4441 agagattctt aacacctata caaatatatg aatatgctga ttttttaaat ggtagactta
     4501 tatttgcatt caatgacgaa aaacaaatat atgaaaatga atttagtttg atgaatgtaa
     4561 ataattcaaa tttcacagta agtgcaataa agaaagaaga aaaaggagat ggaattgtag
     4621 ttagaatctt caatggtatg aaaaatgagg atgctaaggg aagtcttaac atcaataaaa
     4681 atgtcaatga tgcttatagt gcaatgctcg acgaaatcta tgataatact agcaagctta
     4741 aagtaaatac taaaactgta gaggtaaatt ctttatcaca ttgtaaagta caaacaatag
     4801 taattaaata atataaaata gcttatacaa gatttcaaaa taaattgcta gtaccatgtt
     4861 aatagtaaag ccagatatta tgctatttat aagctctgta acactaaata acattttgta
     4921 aaataaaaag gtgaaccttg tatattagga ttcacctttt ttaaataaat caacattctg
     4981 ttttaaaata acatttataa gctgttttga aataatgctt acaaattatt ttaaaacaat
     5041 acctataaat ttttatttaa agaatacaca taaatatttc cagactcttt tatcctatag
     5101 actttataat tattaaagaa gtctaatcta atttttgtta aaagtaattg taactttaaa
     5161 taaatcacca tccacactta tattcatctt accaccctgt aattctataa aactttttgc
     5221 tatcgctaaa ccaagaccag aaccttctgt gttacgtgat ttatctcctc ttacaaatct
     5281 ctccattata tcatctacac taaaatcaat ctcagatgca gacatatttt taagcataat
     5341 atttacttta ttatctgttt ctaaaatatc aatatacact cttgaatttg gcattgcata
     5401 tttgcttata tttattataa gattttcaaa tactctaaat gtacgctgac tatctaatgg
     5461 taatattatt ttttcattag aaaagttatt ctttactatt agagatgaat ctgcaagttt
     5521 gtcatctaat tctaaaagtg tctgcttcat tagagataca atatccacat ttactatatt
     5581 caaataaaca tctccactat ttgccttgct tacttcaaac aaatcttcta ttagatgttg
     5641 caatctttgt gactttctgt ctaatgtatc aatatacaac ttacgatttt catctgttat
     5701 actttcatct tttaataaat ctatataagt gattatagat gttagaggtg tttttaagtc
     5761 atgtgataca tttgatataa gctctgtttt catcttttga ctcttaacct cttcgtcaac
     5821 agctttttta aatccagttt gaatatttcc taattcgtct tttataggat taaaaaatcc
     5881 taaatctttt tccatattta catctaagtt tccatctgaa attttctttg taacatctaa
     5941 aagttgatta tattcatata ttgtcttttt agagtaatct gacagtattt taaataatac
     6001 tactgaatat attaatgcga gtattattcc aaagaaccac atactacaca ttataactat
     6061 aatcaataaa tttattccca aaattgtaac tatagtttta tttggctttt cttttagatt
     6121 tattgttcta atttgattaa taactgtttt tcctgctttt tttagccata caaaaaacat
     6181 tcctgtcaca gtatgttttg taaaatactc tcttattcca acatttaaaa tatgttttaa
     6241 aagtattata aatacaaata ttattgagaa tattacaaac caataagtta aattcattaa
     6301 acttaccaat atactagcca ttttagcact tatgtcattg tttataaggt actctacata
     6361 attcccactc aaggtattac ttattataac agtaggatat aaatctacaa gcccctgtat
     6421 aatatcaaga gcaataaaga gtatctctaa cggtattctt gataatgtat caaatcctag
     6481 caactcttta gattttctat atgggaatgc taaagctaca ataacaacta tagctaatat
     6541 aatttttaaa atcattccag aaatcttata ataagaatta ttttctacat tgcttatata
     6601 tgaaattata ccatcatcaa aataagtatt tccataacca tactctacac tatcaccttt
     6661 acttattact ttaggtacag catatactaa tgttatattt tttattggag ataacttata
     6721 tgaaattgaa tttcctccta aacttgtatc cattaaatct tctcttgctt tagataacaa
     6781 catttgatta ataatttttt gatttactcc ataagaatcc acaatattta cttttccatc
     6841 tttatcaaaa ttcattacaa cgtagaatct atagttttct tttatatctg taatctgctt
     6901 ttgtctttcc tcatcattca tttctgtatt ttttgactga ttttcatcag aattttcttt
     6961 tgaagcatca ttttttgttg tatcaagtac tcctaattct ttattatcat taaaaaattc
     7021 aaaattgctt tctttatcaa ctaaatagta ctttaaattc tttcctgacg ctttaatgaa
     7081 atgctcccaa ttttcaaagt tttcattaaa atctgcatta atactatcat ctgtatagta
     7141 agtttctgaa ccatcataag tatctttttc tttacttaca ttttttatat agttcatagt
     7201 ttcttcattg ataaatagtt ttgatggttg tatcatctta ccttcttgtt cttccttcat
     7261 attatgatat aaaacataac ttcctttata tatactatct acaaagcttt cctgttcaaa
     7321 tggattctct gctccacctt tagcacttgc ttgtatagta gaatattgac taatcatacc
     7381 taatgatgct gataacataa gtataaaaac tataatagta actatatatt tatctttaaa
     7441 ttttttcaat tttgtatcca acaccccaca ccacctttaa atatttagga ttttttgggt
     7501 caatttctat cttttccctt atattcctca catgtaccat aactgtatct gtatttacag
     7561 cattctcatt ccaaactctt tcatatattt catcagcaga aaaaactctt cctgcatttc
     7621 tcataagtaa acttagtatt ttaaattcta taggagttgt ttttatatac tttccatcaa
     7681 tactaacttc ttttgtattt tcatttaatt ctaatccgcc tattgcaaac acactatcgt
     7741 cactttctac cttttgctct ttatttccaa ccatgtttag atacttagtg taccttctaa
     7801 gttgtgaatt aactctagcc aatagttcta atggcttaaa aggttttgtt acataatcgt
     7861 ctgctcctat attaagcccc attattttgt caacttcctc tgattttgca gatagcatta
     7921 ttactggaaa attatagttt tctctaagct tcattgtaag tgttatacca tccattttag
     7981 gcatcattat atcaactata gctaaatgta tttcttggtt ttctataacc ttaagacctt
     8041 ctaatccatt gtatccctta aaaacattat atccttgatt taatagatat atttctatag
     8101 catctgctat ctccttttca tcttctacca ccaaaatatt atatgaattc attctaatct
     8161 cctttatatt aatttataaa ttttctttac atatatgatt atacaatatg atagaagttt
     8221 ttccatttat atatatattt atatcttttg ttctggttat ttgtatttaa tgtccatctt
     8281 agaaacctcc ttctagttta aatttaatat tttttcattt tgttttatat acttaatatt
     8341 aaaattaaaa acttaagata ttacatatac ttttctaaag attttctaaa gatttaaata
     8401 tatttaaaat taatatacaa aaaaagtagt tcttattgta ttaattaaat caatgatttg
     8461 aaatataaaa ttaatatcaa taaaacctac tttttaattt tttaaatatt tatttttaat
     8521 ttattttgtt tttaatttac ttcgatttta aaatgtcctt gacttttatt ttataaatgt
     8581 acatatttct tctactggtt ttttagatgg actcttcaaa ttgtcctctg gcactccaag
     8641 agctaacatt gcacctaaaa aatactctcc cttttcaaaa ccagtttctg cttctaaaac
     8701 agcttctatc tcatctgctg catagttttg acttgtcaac caacaagaac catatccaag
     8761 ttcaattgct gatagtgtaa agttttcaat tgcagcacct agactctgca tacctggatt
     8821 tcttataaat aatttatcta tagtctcctt aggtgcatct ataagctcta attcataata
     8881 tcctgatgga ttatatacct ttgtaaaaac taatactaaa actggagctt ttaagtaaaa
     8941 tagtgtaaaa tttttaacaa attttctaaa tctattagcc ttatccactg atactttatc
     9001 catttctaca agtatttctt gttgcttttt agttatcaca tctgctatct tttccattaa
     9061 atcacggcgt tttataacaa caaaatgcca gttttgaata ttcttgcctg atggtgctgc
     9121 acctgctgct tttatcattt ttaaaatatc ttcatctgat acatcttgat ttttaaactt
     9181 tcttacactt tgtcttttaa aaatagtatc ttgtaattcc atttttaaat ccccccataa
     9241 cttttttatt attttaatat aatttttaaa ctcatcttat tttaatatat catgttctaa
     9301 caaatcttta aatgaatcta taaaataatc atatacatct acattcccaa acccatattt
     9361 ggcatatata aaaggtatac ctacaaactt agcactattt gcatcacctt gcgtatcacc
     9421 aacatagatt ggatttatta atttatttct ttcaattata agtttattat tttctccttt
     9481 atgcaaacca gtattaccag gacattcaaa atctataaaa tactgttcta gtttatgtgc
     9541 ttttaaaaaa gattctatat atccaacttg acaattactt actataaata atttatattt
     9601 tttagatagt aatttaagag tatcttctat atcatcaaat aatttagctc cattttcaga
     9661 taaatagtca cattctttca aagaacattc attcaataaa ttcattcttt cttcttcact
     9721 taaatttgga aataaacgat ttgctatttc ctcaaaagaa agccccataa gacttctaaa
     9781 taaatctttt gtgacactta attctatttc cttatgctca cttaaaacat cctgccagac
     9841 tttgcatact tcttcagttg aatcccacaa tgttccatct aaatcaaaaa taatactatc
     9901 taccacaaaa aatccccctt atgtcaaagt ttattatata tttataatta tatactaaaa
     9961 agcagataca atcataaata tattttactt gattgtatct gctctacaat ataaaaccaa
    10021 ttgtcaaaat caaattttat aaatttctaa ctacaagtac acagccttgc tctactttaa
    10081 tcttacaaga attactcaac ataacatttg atattccaat aggtacagag tatttcatat
    10141 aataattatc gagagggtat tctaagccag taagtgttac acccttagca tcaccattta
    10201 tagctattac agaaatagtg tcccctgggt ttccatacaa tgaaatttcc tcattttcaa
    10261 ctatatacat ctcttcttta tcagaaagta tcctagaata tataccatct tcttttaaat
    10321 aatataatag ctttatattt gcaagagtgt ggtcaatcct tccccctaaa gcaccaaaaa
    10381 aatcaatatg atttgcttta agtgtcttag ctaaaaaaag acaaagctct gtatcagtct
    10441 catctttttt agatggaaat ttttcaaatt taacaccttt atttttatag aagtttatct
    10501 tttcttcttc tactgaatct aaatctccta atatataatc tggcattatt tccattttat
    10561 aagtgtgatt tgcccctcca tctgcacata ttatgcagtc ataacattcc ttaattataa
    10621 tatctcttgt aacttcgtaa tcttcaattt cgccatttaa aacaatacag attttcatta
    10681 ttaaatttaa actaattctt caagagaagc attctttcta aataaattaa ctgtttcttg
    10741 aatgtcatca cttccaaata ttgcagaacc tgctactatt acattagctc ctgcttgtac
    10801 aacttcggct acgtttttag ggcttatacc accatcaact tctatatcaa tgtttaatcc
    10861 ttgactatct ataatagctt ttaactcttt aatcttatct aaaacacaag gaataaatga
    10921 ttgtcctcca aatccagggt taacagacat taataatacc atatctaaat caggtagtac
    10981 atgtttaatc gtatctactg gtgtagctgg attaagtact acaccagctt ttattccatg
    11041 tgattttata ttttgtatag ttctatgtag atgaacacaa gcctcctggt gaacagttat
    11101 tatatcacat cctgctttta caaattcttc aatatactgg tctggatttt ctatcataag
    11161 atgagcatca aataccatat ttatattttc tttctttaaa cttttgacta caagtggtcc
    11221 taatgtaatg tttggtacaa agtgtccatc cattacatcg atatgaagat attcacatcc
    11281 tgcactttct acttttctta catcttctaa taattttgca aaatctgctg ataatattga
    11341 tggtgctaat ttaatcatgt ttctaatacc tcctactatt attttgtctt atttcatgta
    11401 gcagctgtat ataactatca tatctttctt tagatatttc tccatttgta acagcttctt
    11461 ttatagcaca attaggttca ttttcatgaa tacactttga accaaactta caatcattaa
    11521 atttatcaaa ttctataaaa taatctttaa gttcaacttc ctctatatct tcaagggcaa
    11581 gggaactaaa acctggtgta tctgcaacca ttccaccaaa ttctaatttt aaaagttcag
    11641 cgtgacgagt agtatgtttt ccccttttta ttttatcact tacaactcct gtttgaagtt
    11701 taaaattttc atctatttca tttaataagc ttgattttcc aactccagat ggtcctgcaa
    11761 acactactac attttctttt aattcttctt ttactttatc tatatttaac ttagtaatat
    11821 tgcttacagg aatgacttta tagccactaa gttcatatat atttttaaca gtttctaatg
    11881 tatcattatc atctaaatct gcttttgtaa ggattattac agtttctaaa ttttcttttt
    11941 ctgccaaaac tataaatcta tctaaaagag ataagtttgg tttaggattt tttattgcga
    12001 atactattaa tgctttatct acatttgcaa taggaggtct tataagttct gtatctctac
    12061 tgtcgatttc ttctaagata cccttttttt cattttcatc aacaatgctt attttgactc
    12121 tatcaccaac caaaggtgtt attttctgct ttctaaatat ccctctagcc ttacattcat
    12181 aaataccatt ataagtatct acataataaa atccactaat cccctttata atttttcctt
    12241 ctagcatcaa atgcctccct ttataaaatt tatataaaca catactgttt atgagtagta
    12301 tatcgtgtta ttctttccta tttttatata ataccacatt ttttttaaat aacaaatcct
    12361 ccgtttggac taattacctg accagtgata aaatttgatt tgtctgatgc caagaaaata
    12421 gcacaatttg ctatatcttc tggagttcct agcctcatta gaggggtttc ttccacaaga
    12481 gcgtctatat cttcctcatt atatccagat aacatgtcag tattaatgac acctggagct
    12541 atgctattta ctctaatatt tgatggtcct acttctttag ctaatgcctt tgtcatacct
    12601 attatgcctg cttttgttat agaataatgc acttcacaag acgcacctga tataccccag
    12661 attgaagata tattaattat atttcctttt ttttctgata tcatgtattt taaagcaact
    12721 tgtgaacaat aaaagcttcc ttttaaatta atattcatca tattatccca atcttcatca
    12781 gttatatctg taaacaactt atcttgactt acaccagcat tgtttacaag tacatccaat
    12841 cctccaaatt cttttataca ataatctacc atatcctcta catcttctct atttgaaata
    12901 tcagccttaa aaagttttac agaaaaattt ttttcattta atatagtgta caactcttta
    12961 gcttcatttt ctgacttatt aaaatttact aatacattgt aaccttcttt tgcaaaagct
    13021 ttagacatag ctttaccaat ccctctagct cctcctgtaa ttaaaactgt tttcttcttc
    13081 ataaatactt ttctccttat attataacca attcagtatt ttatacaaaa caaactgccc
    13141 tttaaagaaa atattttcta taaaaggaca gctcttattt tattaatatt tattctgttt
    13201 taccaatgtt tgcgccagtt cctgatgaag ttcctccatt atctcctgag tttgagcctc
    13261 ctgaactatc tccattgtct cctgagtttg aacctcctga acttcctcca ttgtctcctg
    13321 agtttgagcc tcctgaactt cctccattat ctcctgagtt tgagcctcct gagtttccac
    13381 cactatttcc tgagtttgaa cctcctgagt tacttccatt attgcctgag tttccaccac
    13441 tatttcctga atttgagcct cctgaatttc caccactgtt tcctgagttt gaaccaggtt
    13501 ctgttggtgt attattctct ggtgttgttg gttttttatc tggtactgtt ggagtctgtg
    13561 tatttgattt ttctgaacct ttgcttacat ataggtttac ctcatcacct tcctgagctc
    13621 ttgatgagcc agcacccgga ctctgactta aaactgtacc ttctttatat attgagctgt
    13681 attcatactt aacagttccc acttttaagt tattttcttc aataagttgt tttgcattac
    13741 tcaaagtaag tcccactaca ttaggaacag atgcctgact tgaaccttta cttacgacaa
    13801 catttatagt atcacccttt ttgacatttg tacttccacc ttcaggtgtc tgtgatatta
    13861 tacaattttc atcaacttca ctattatact cttcactttt tacactcagg tataatcctt
    13921 ctttttcaac agcactttga gcttcttcta atgtcatatt tacaagatta ggaacagtca
    13981 aagatttatt gccaaaccct ccagcaaata aaaacttata tgctaagaat acttgagcac
    14041 acaatatcag tatcaaaaca gccgcaatta cttttaatct tcttctagat tttgggcttt
    14101 cctgtttttt cttctttttc ttagttctct tacttggagt actcttaggt ctctgattct
    14161 ttttagctct cattatctct tcctcttctt catcttcatc atcatcttct tcataaaaat
    14221 catcatagta atcttcatca tcatccaaat cagctacttc aacaggctta accacttttt
    14281 caggagctgg ttttgcaagt gttggattta caactttatt aatttctttt tcatcaattt
    14341 tcttagttgc aaaatcatca tattctttta taaaatctaa atcaatattt ttttctatat
    14401 actctatatc ttctatcaat tcttcagcag tttgatatct atctgcacta gatttttctg
    14461 taagcttttt tattgtagtt cttacacttt gaggaatcct aactttttct tctgatgtaa
    14521 agtctatatc atcattaatg tgttgaagtg ctatagaaat tggactatct cccctaaatg
    14581 gaacttttcc tatcaacatt tcatataaaa ctatacctag agaatacagg tcagcattat
    14641 tagttacaaa tttaccttta gcttgctctg gtgaaaaata atgcactgaa cctattatac
    14701 taccaatatt agtcattgtt gaatttgaaa cagctttggc tataccaaaa tcagccactt
    14761 ttactactct tccttcattt gatatgagaa tattatgagg ttttatatct ctatgtataa
    14821 tccccttttt gtgcgctgca ctaagtgcca tagctatttg ttttgttata tctaatgcag
    14881 tatattcatc taatatacct tcatcttgta taatctcttt tagattcttc ccatctacaa
    14941 actccattac aatgtagtga acttttccat cttcaccaac atcataaaca tttactatat
    15001 ttggatgtga aagacttgct actgcttctg cctctctttt aaactttgtt agaaattcat
    15061 catcatctac aaattcaggt ctaagtactt taagtgcaac agtcctgttt aataatctgt
    15121 ctttagcctc atatacaaag gccattcctc catcaccaat cttcctgatg atttcatatc
    15181 gatttcctaa aattgtatct cccacaatat caccgtccta attatacttt tatcactata
    15241 accgaaacat tatctttacc agaaacatca ttggccatac ttatcaattc ttcacttata
    15301 ttactagttc tctcatattc ataatctaat attaaatctc ttatatcttc atcatcaata
    15361 aacccagtta atccatctgt cgcaagaagt attatatcac tttttttgat ttctttctta
    15421 aaaatatcta caacaaccat atcatctgtt ccaattgctc tagttattct attcctctgt
    15481 ggatgtcttc ttgcctcttc ttctgtaatg acatgggcca tcataagttc ttcaaccacg
    15541 gaatggtcta tagttatttt atcaaatttt tcatctgtta acaaataaca tctgctatct
    15601 ccaacatttg ccacatataa gacattatta tacactatag ccgtaactaa tgtagttccc
    15661 attccttcaa attctatatc ttccatagat tttttgtgca caattgagtt tacattatta
    15721 taagcctgtt ttaatatatc atctatatag tctattttta cattatcatg ttgtaataag
    15781 ttttccttta gaaaatcaat tatattttct actgctaatt tactggcgac ttcacctttt
    15841 ttatgtccac ccattccatc agctatggca aatattccta ttggaccatg ttctgtatct
    15901 ataacttctc cctcacaata gtcttcattg ttttttctta tttttcctat atgggaggca
    15961 caactataaa ccatacgact tcctccttac aaattacttg tattttcttc tcaattgccc
    16021 acaagctcca ctaatatcag aacccataga catcctaact gtggcaggta tattgttctt
    16081 ctccaaacta tccctaaatt tataaataaa tgctttatct ggtttttcgt attctctttc
    16141 ttcaacttta tttattggaa ttaaatttac atgacatagc atacctttta ggagcttagc
    16201 taatgccttt gcttcttttt ctgagtcatt tactcctttt ataagagaat attcaaatgt
    16261 aacccttcta tttgtctttt ttatataata tctacatgca ttcaatattt cttcaataga
    16321 ataagcattt gctacaggca taatttttct tcgttcttca tcatatggtg aatgaagtga
    16381 taaagctaaa tttatagcta tttctaagtc tgccaactca tacattttag gtactattcc
    16441 acaagttgaa agagttatat gtctataccc tatattaaga ccattttttt cgttaattat
    16501 ttttaaaaat tgttttgtat tttcaaagtt atcaagtggc tcaccactcc ccatcaatac
    16561 aagatttgat accctcttac cagtatcttc ttgaatttta ataacttggt ctaaaatttc
    16621 ccatggctct aagtttctaa ttaatccatc cattgtagat gcacaaaaat tacatcccat
    16681 cctacaacct acttggttag aaacacagac agtaactcta ctatcataat ccatcatcac
    16741 agtttctatt atatttccat catctaataa aaacaaatat ttttttgtat tatctacctt
    16801 agattctaac tttaattcta tatctatatg ccctatacat gaaacctctt ctaatttatt
    16861 tctcaaactt tttggtatat tgttcatatc atcaaaagtt tttgcaccct tatatatcca
    16921 agagaatatt tgacttcctc taaagggctt ttcatctata gttttcataa actctttaag
    16981 ttcatcttca gtaaaatttt ttaatactat ctttttcttt tccattctct cactacctta
    17041 ttctctttaa ttttgctatg aaaaaaccat ctataccatg aatattagga tatatcttta
    17101 aatatccttt atcttgattg tctaagtcta catttactgt atcaattgga acaaattcaa
    17161 agttgtgatt ttcttcaaca aaagaagtaa ctatatttat attctccaca tcttgaattg
    17221 tacatgtact atatactaaa gttccaccaa ctttgacata tttagatgca ttttctaaaa
    17281 ttttcttttg tatagaagtg atgtctttta actcttcttc tttcttatac ttaatttctg
    17341 gtttacgtct tattatacca aaaccagagc aaggaacatc tgctaaaaca taatcaaatt
    17401 tacttatact attttcatca atctcagaag catcaaatct ttctacaaac acatttttaa
    17461 gccccaatct atttacagta gagtttataa gctttagttt atgctcaaaa atatctctag
    17521 ctactacttg accagtattg ttcattaaag tagctaagtg agtagatttc cctccaggag
    17581 cactacatac atccaaaaca aatgaacctt cttttggatt aattactttc cctactatca
    17641 tagaacttat atcttgaatt gtaaataaac cagccttaaa taattcattt ttttctatat
    17701 ttttcatttt ttcgacttta atagcttctt ctaccatagg aacttttaaa catacaatac
    17761 ccatttcact taatttttca ataagttctt ctctacttat tttaagtgta ttcgttctta
    17821 tatagatact aggcttttca ttatttgctt ccaacaaatc ttcagcaaat tcttgtccaa
    17881 atttatctat ccagtttttt ataatccaag agttatagga atactttata gataagtatt
    17941 ttacactatc ttcctcagtt atgtccatta tagtctcttt agctcttatt tcatttctaa
    18001 gaatagcatt tatgaaacca gaagattttt tatcatattt cttagataat tttacagttt
    18061 cattaactgc tgcataatct gatatactat ctaaaaataa aatctgatat gttcccattc
    18121 ttaaaaagat tttaacataa gttgacattt tttttacctt tatttttgaa agtttattga
    18181 ttatataatc taaataatat ttattttcta ttactccata aattaactct gttgcaagtc
    18241 ctctatccat atcacttatt tctaaatttt taaaatgctt atttattgca atatttgaat
    18301 aattgtcatt tttttctata tcacataata ctttaaatcc aacttctctt gcatccatat
    18361 aaatttctcc cttttattaa acataaaaaa gggttgtttc taaacaaccc actttacttt
    18421 tagtcatttc ttctttgagc tattagtaac aatctaagta attgaagtat tgaaaccaat
    18481 gctgctgcaa catatgttaa agctgctgct gaaagtactc ttctactttg tcttgactca
    18541 ctttcatcta taatccctaa attcccaagt tgaacgattg ctctacttga agcattaaat
    18601 tctacaggca atgttattat ttggaataac actgaagccg aaaacaatag tataccaatt
    18661 tttaaaaatg gaccagccat aataaatcct aaaaatatta aaaaccaaga tatacttgaa
    18721 gcaaaattca ctactggtac taaactactt cttatttgaa gaggtgcata acctttagca
    18781 tgttgtatgg catgaccaca ctcatgtgca gctactgcaa cagaagttat agatgttcca
    18841 taatatacat cttctgacaa ccttacagcc tttcttcttg ggtcataatg gtcacttaga
    18901 tgccctctaa ccatttctat ctttacatca tacaaaccat ttgaatcaag tactcttctt
    18961 gctgtttgct ctccagtata tcctcgacgt gtatttactc ttaaatactt atttgttgtt
    19021 gaactcactt taaattgtgc atatatagta agtaatatag ctggaattaa tacaaccatt
    19081 gttgggtcaa atccccaaaa accgccatat acagggtaca taaataacac tctcctaaaa
    19141 tatattttta ttatacatcc tcttaatatt cacttcactt aaaaacagtt taattacaaa
    19201 ctaatatatt ttttcctgtt aaaaagccta tagctttttc aatatccata atgtctacat
    19261 tagtatttac acaaggtcca ttaggtcttt tgttaaatac acctaaaaca ggaattttct
    19321 taatatcttg tattccagat gtcaaatctc tttcacatgc aacagctaca acagctttag
    19381 gtttattatc cattataatt tttctagcta gggttcctcc agtagctaca aatattttta
    19441 cattagtttt ttcctttagc ttaattaatt ctcctatgtt gcatcttcca cattctttac
    19501 aattatctat atcattagta acttttaatt tacaactatt ctcttgaata caatgaggta
    19561 ttaaaattat tatatcctct ggattaaaat tatatttatt actgtatata tattcattat
    19621 ttaactttat atatattctt ctaatatcac tttttgacat accaaatgat gatgcaatga
    19681 aagatataat aggaaataat ccactgacaa tattaaaatt tattttcatt atatttgagt
    19741 taacatactt tcctttaatt actctataag taactgctgt tgaacatagt attataagta
    19801 ttagtgatat tattataata tttatcatca aggagaatat ttgagttaaa cttattataa
    19861 aaaaattaac aactcctatc aaaattccta atattaaaat caaaatccca acacttgtta
    19921 aatacttctt tatatctgtc atacttttca cctaacccaa tactaaacca gtttctatag
    19981 tattcccttt tatatattca ccaaccaaca ttctcttttt attcggcatc tgaatttctt
    20041 caattaaaag tacattatct atagtactta cttttatccc atctttgctt acatcaatta
    20101 ttgtacctac atcttttgtg cttttttcat ctaacacttt agttttccaa actttcatta
    20161 tgacatcatt ataggtagta taggcacttg gccatggatt tactcctcta actaaattgt
    20221 gtatttctct ggcacttttt gaaaaatcta tgttacctaa tgatttattc attattggcg
    20281 cataggaaaa ttcttcatga ttttgtactt ctcttggagc atttccttct tcaattaatc
    20341 ttaatgtttc ttttaaagtt tctgcgccta tattcatcat tttatcatga agttctcctg
    20401 ctgttatatt ttcatcaaga tttacttctg tctttagtat catatctcca gtatctagcc
    20461 cttcatccat atacatagtt gtaacaccag tcttttcttc accatttatt attacccaat
    20521 ttataggtgc cgcacctcta tattttggaa gtaaagaaac atgaacattt atacatccaa
    20581 gtttaggaat ctctaatatt tcttttggaa gtatctgtcc aaaagctaca actactatta
    20641 catctggatt taaagattta attttatcta taaattcttt atctctagcc tttactggtt
    20701 gatatacagg tatattattt tcaattgcca gttcttttac tggactcata cctagttttt
    20761 tacctcttcc ttttggttta tctggttgag ttacaacacc caatatttca tacttctcat
    20821 ctattatttt ttgtaaacat ggaactgcta tatcaggagt tcccataaat actattttca
    20881 tcaaatcaac tcctactact tttctatttt gtcaacaaac aaaattccat ctaagtggtc
    20941 tatctcatga caaaatgctc tagctaaaag ttcttctccc tctaactcta ttgtttcacc
    21001 atttctattt aaagctctga cctttacata atttggtctt ctaacatttc ccgcttctcc
    21061 aacaacactt aaacatcctt cctcatctat ttgctcccca gatgtttcta ttatttcagg
    21121 atttataagt tctataagct cttctccaat atctattact actactcttt ttaatattcc
    21181 aacttgaggt gcagccagtc caacaccatc tgcatcatac atagtttctg ccatatcatc
    21241 taataattgt attatctttt catcaatttt ttcaaccttt tttgattttt ttcttaatac
    21301 tggttctcct atttgaacta tttgtcttaa agccattata attcctccta aatttataat
    21361 acactgtttg gattaatatc tatagataca ttgatattct tatcaaatat caaatctcgt
    21421 tttgttatac atatatattt tattataccc tttaagagat taatttcaat attttcatct
    21481 ttaaagagta tttgccatct ataattttga tttatttttg atattgaaca tggatttgga
    21541 cctaatataa atcctaaatc atttataccc cttttttcta ataaataaat caatgatgca
    21601 tgcatatttt ttatattttt tatcactagt ctttcatctt caccactaac aactacactt
    21661 aacatattat taaaaggaga atatccaaat gcttttctta tttttatctc atcttcataa
    21721 aaaccctcat aatcatattc aatagcatgc ttaatagcat agtgttcagt gtcataagtc
    21781 tgaagtacta cttttccctc tttatcagct ctccctgctc ttccagagac ttgagttatc
    21841 aattgaaatg tagtttcaaa acttttaaag tctggaaaat ttaatatcat gtctgcagac
    21901 aaaataccta ctaaagttac attctcaaaa tccagccctt tacttaacat ctgtgttcca
    21961 ataagtacat ctgcttcttt atctttaaat ttatttagta tctcatctaa agcacctttt
    22021 tttgaagttg tatctttatc cattctcaaa gtttttatat ctggaaaaat atattttaat
    22081 tcctcttcaa ttttttgagt tccaactcca aatggcttaa cataagtact tttacattca
    22141 ggacactctt ttggaatttc tttctcatat ccacaatagt ggcatcttcc agtattgctt
    22201 ttcttatgat aagttaaaga tatatcacaa ttttcacatt gaaacacata tccacacttt
    22261 ctacaagata caaaatttgc ataacctctt ctgtttaaaa atagtattac ttgattattt
    22321 tcttcaatcg cataacgtat ttctttttgt aatttcaaac taaatatact tctatttcct
    22381 ttatctaact catctttcat gtctaccact tcaatgtttg gaagaggctt atcatttgct
    22441 ctacttttta tatttataag tttgtattct ccagattttg ccctataata ctcttcaatt
    22501 gagggcgttg ctgaacctaa aataagagtt atattatttt ttaatgccat aaatctagca
    22561 acttctatag cattaaattt tggattcttc tcagacttat atgatgactc atgacattca
    22621 tcaattatta ttaagcctaa ggaattaaaa ggtgcaaata aagctgacct agctccaatt
    22681 aacactctta ctttacctgc tttgacagct ttgtaaacat catgtttttc tccttcagat
    22741 agtttactgt ggaacactcc aaccaaatct ccaaacctac tctttagcct atctattgtt
    22801 tgaggagtta atgctatctc aggtactaag aatatactgt ctaagccttg atttagagca
    22861 aattctatta tttccatgta aacttcagtt tttccacttc cagttactcc atgaattaag
    22921 tatggttttt tatcatctac aaacatattc gactttatct catcaactgc ttcttgttgc
    22981 tcattgttta attttatact ttttagtacg gatttgtaaa tactttttgg ctctctataa
    23041 taatccttaa attctagggt tattagcttt ttcttactta gagaatttat actttgtttt
    23101 gaagcattta ataaatccaa taaatcattt atctctacat tttcattatt tttaagaaaa
    23161 cttaatatct ctttttgttt acttcccaca tttattttat tttgctctat ataatcgtca
    23221 atcttatcac tttctaaact caaagatata taacatacct tcttttcatt tttgtgattt
    23281 ttatattccc aacataattt tataattcca ttcttattca ttgtgtacaa agaactattt
    23341 aagtttggta tatattttaa cttatcaacc ttaattttac ctttactatc aatcactttc
    23401 tctactattt ctctattttt atcacttaaa gttgatattt tttccttcaa ttcaatatca
    23461 tttaatccac ttaaactttc acctaatagg actactttat aattatttaa tttgtatccc
    23521 tttggatata tcaaatttat acattctata taagtacaca aatatctatt tttcatccaa
    23581 taaaccaact ctaaatcttc ttttctaaat atcggatttt catctaatat atcaataatt
    23641 tcttttattt ttattttttc atctaagcta tctgtaaatt gaaatacaaa ggcttctgtt
    23701 ggtttattac cttttccaaa tggtactaat attctgtgcc caatttttat tacatcagat
    23761 aaaaatacag gaatttgata tgtaaataaa ttatctgtat atatagtatt acttctaact
    23821 attacctttg catatttttt cattataaaa cctctactta aatttaatat attgttcata
    23881 ttttaatata ctttacaaga tattaaaatc tcagaattta taagcaattt caagttatta
    23941 atattaactt tattttaata attaactatt aattatattt tatactttat gacaaaataa
    24001 atgctgaaaa ctacaattta actgacattt gcgagttttt actcgctaaa attaaattgc
    24061 agctttcagc cactttagct gtgataaaat acacaaatat catatttaaa aaaggtctta
    24121 tttctcaagc aacgctttaa ctttatctaa aataacattg gctacttctt cttttttcat
    24181 cttaggatac tcagtaatat ttccctctac atctattatt tttacaatat tagtatcagc
    24241 tctaaaacca gcaccttctt tagttaaatc atttgccaca ataaaatcta aatttttctt
    24301 ttttattttt cctttggcat gttctataag gtcattagtt tcagctgcaa atccaattag
    24361 tatcttatct tcttttattt ttcctatttc ataagcaata tcataatttc tacttagttc
    24421 tattgataaa tcatcattct tctttttaat ttttttgtca gaataagttt ttggtttata
    24481 atctgcaact gctgcacttt gaattactat tttattttca cttaaatttg atactactgc
    24541 ttcatacaaa tcttgtgctg actctatttg aacaaatttt ttaagatttt gtggaataga
    24601 aagatttgta ggacctgata ttaatgttac atcagcacct ctagctatag cttctcttgc
    24661 tatagaatat cccattttac cagaagacct attagttaaa tatcttactg ggtctatact
    24721 ctctacagtt ggaccagctg taactatcat actttgacct tttaaatctt gctctttagt
    24781 aagattagtc aaaacttcct ctactattat atctggatta gctagtttac ctgttccaat
    24841 atctccacat gcaagccttc cactttccgg ttcaataaat ttatatccaa agctctttaa
    24901 agtttgtata tttttttgaa caattggatt ttcatacata ttagtattca tagctggtgc
    24961 tataagaacc tgtgcttttg ttgccataac agtagttgtc aacatatcgt cagctatacc
    25021 atttgcaatc ttgcctataa catttgcact tgctggcact attaagaata aatctgcttt
    25081 ttttgctaat gatatatgtt caacttccca agtttttggt tcttcaaaca tatcttctac
    25141 tacataattt tgactcagcg attgaaatgt aagtggttta acaaattctg ttgctgattt
    25201 tgtcattata acatggatat ttgcatttag tttttttaac ttacttacaa catcacaagc
    25261 cttatatgca gctataccgc cacttatgcc aattacaaca gttttatctt ttagcatgac
    25321 cttactcctc ttctgaaatt tgttggtgtt gctcagcatg atgtctagct tcttctatct
    25381 ctatttcctc ttctgtcaat aatctatatg ttatttttcc acttgcaact tctttagttg
    25441 ctacacatac aggcttttcc ttagttttat ttgaaacata tggttcttct ccatcaatta
    25501 attttcttgc tctctttgaa acagtcccta ctaaatagta tctgttgtct attttttcta
    25561 gtacctcatt gatagatggt tttaacataa ttataattcc tccttaaatt tctctattat
    25621 aatattttta tatctgctaa ctttattctt ttcagatgat attatacctt ctatttcttt
    25681 agcagatgtt tttacatctt cattaaatat aaaataatca taatccttaa tcattttaat
    25741 ttcttcatat gcacagctaa atctcttctc tatttcagct tctgtttctg tacctcttcc
    25801 aacaattcta ttttttaatt ctttaagtga tggtggtagt acaaatataa atactccctc
    25861 tgggcaaact tcttttattt gttttgctcc ttgcatttca atctccagta atacatcttg
    25921 tcctttttct aaacattcca ttattgccgc ttttggagtt ccataaaaat tatcataaat
    25981 ttgagcatac tccaaaaact cacctttttc tatcatctct tcaaactttt ccttacttat
    26041 gaagaaataa tttactccat caacttctcc agttctgggt tttcttgtag tagctgatac
    26101 agaaagtttt attgtatcat tttctttaag tagttctttg caaatagtac cttttcctgc
    26161 acctgatggt cctgatacaa ctagcaataa tccttttttc acaaacattt aagcattccc
    26221 cttctaatat tgtattctta ctctatattt tgtatttgtt ctcttatttt ttccaattcg
    26281 ctttttactt ctactactaa attagttata tttatatcag atgacttcga acctatagtg
    26341 ttagtttctc tattcatctc ttgtattaag aaatctattt ttctacctat agagtcgtct
    26401 ttaaatatag tatttttaag ttgccctata tgacttttaa atctaactat ttcttctgtt
    26461 atactgcttt tatctgcata tattgcaact tcttgtgcta atctattctc atctattata
    26521 cttgggtcat ccagtaattc acttattctc aaattcaatt tttctctata atcttctaca
    26581 acactacttg aatatttttc aatctcttct atatgatttt ttaataaatc acatctcatg
    26641 gcaatatctt cagctaattt tttaccttct tcacttctca tttctcttaa tttgattaat
    26701 gcatcttcaa ctgcttgatt caacattgac cacaataaat cttcatcttc ttccttttct
    26761 tctattttca caatatcagg aaactttgca acattcataa cgctaatatc atctataata
    26821 tcaaatttat tttttatttc ttttagtatg tctatatact gagatgctaa tccctcatca
    26881 aaatttaagt ttacatcttc tttgcctaat aagtcaaact ttatgtataa atctactctt
    26941 cctcttttta cataattttt tactaaattt ctaactttat cctctaaaaa tgaaattttt
    27001 cttggaagac gtatgtttat atctgaatac ttatgattta tcgttttaca ctctactaaa
    27061 aagtaataat tatcatcctt gtattctcct cttccaaaac cagtcatact tatagccatt
    27121 aattaaacct ccaaaatacc ttcacatatt tttactgctg gacctgtcat ataagagaaa
    27181 tctcctacat ctattttaac tgttccacct tgagacgtta catttacact actttctacc
    27241 atattaagta aattgcacac tattgctgat gcagtcatac cagtaccaca acctaaagta
    27301 tatccacaac ctctttccca agtataaaca tgtatattat acttatcttc tacctttaca
    27361 aaatttacat tcgttttttc aggaaatatt ttattatttt caatctcttt tccatattta
    27421 caaacatcat ctacatctaa ttcatctact ataactactg tatgaggaac ccccatcaac
    27481 atagaagtaa ctttaagctc cctatctaaa acctttatac tttcttgcac aaaacgttct
    27541 ttatttgtaa gtacaggaac gtccttagct ataaaacttc cttttcccat attgactctt
    27601 atagagttta tttctctatt agtaagattt acttttatat cttttatacc atctaatgta
    27661 tctactgtaa aactttcttt tttaactata caattatcat aaacaaattt acaaaaacat
    27721 ctaaggccat ttccacacat agctgctctt gaaccatcag aattataata taccatttct
    27781 acatctgcaa cttttgaatc tttcacaact aacaatccat ccgctcctac agaaaatctt
    27841 ctatggcata ctttttttgc taaagcagta taatcagatg gatttatctg gtcgaatctt
    27901 ccatctattg ctataaaatc atttcctatt ccatgtagtt tccaaaattt cataagtatc
    27961 gctccttcgt aaattaacac ttaaactatt ataacataaa aaaattccca taaacttata
    28021 atttatggga aaagttttgt aatgacaact ataatttgag gatgtgaggg agagttgcca
    28081 ttacaattag ggtaattata ttatgttttt aagctagttc tttctatgtt tatatattaa
    28141 tatttctata tgaattttat gtatgtttta tttaaactgt ctatgaattt tttaattaaa
    28201 catctggtaa ctagctataa tccttctagt agatatctat taacatacac ctattaattc
    28261 tttaagtgtt tttaaaacat agtctaaatc ttccttagta atatctacat gagttacaaa
    28321 tctatattca ccattttcca ttttattaat ttttatattt ctttcaaaaa attcttttat
    28381 taaaacatcc tcttttataa tatcttcact catcttaaaa aataccatac ttatatctgg
    28441 attaacattg tttacttcaa tgccatcaat tttatttaat tcacaagcta aatattttgc
    28501 attttcatgg tcttcattca atcttaaagt cattttttca atggcaatta taccagctgc
    28561 tgcaactata cctacttgtc tcattcctcc acccataact ttttgctttc ttctagcttt
    28621 tgctataaat tctttatttc cagcaagtat agaacccatt ggtgcagcta aaccttttga
    28681 cacacagaac ataacactat cacaacactt tgctatttct ttagcttcta cacccaaagt
    28741 taatgctgcg ttaaataatc ttgctccatc taaatgtact ggtaagttat gtttctcagc
    28801 tattttttta atttctctta aattatcaag actcacaact gttccacctg aatatgcatt
    28861 ttctacacat ataagccctg tctgtggttc atgtatgtca tctcttctaa ttgccttttc
    28921 aacaagttca ggatttagag ctccactttc gtcttcatct atagttctaa gctgaactcc
    28981 agctattaca gcaggtgccc caacctcatg agtcacaatg tgataacctt taccaataat
    29041 tatttcttgc cctcttaggc aatgagtaaa tagagctaat tgattcccaa aagttccact
    29101 tggaaggaaa atagcatctt cttttccaac tttagctgct gctaattctt caagtttatt
    29161 tacagtaggg tcatctctaa agattgaatc tccaacagga gcctttgcca tagcttctct
    29221 catttcgtca gtaggcatag taacagtatc acttcttaaa tcgataaatc tcattttttc
    29281 aaccttcttt catatttata tactatagaa ccttaaaatt gattttttct gtaaagttca
    29341 tatactttca tagcttatac tctacacaat aattatacta tatttacttt atatttaatc
    29401 tactttaaat tattaatttt aaaaataaag tttcctctct aataaatata aataattata
    29461 caatttaaat attaatctct attgtttaaa ttccccctag tcacaaagcg tatattatgt
    29521 gtataattaa tttactgact cattttggtc aaagaaaggg gtgctataaa tggcattaga
    29581 tggattagtt atacattcaa tagtcgatga attatcatca aaacttacag gtggaaaaat
    29641 agataagata catcaacctg aagatgatga ggtaattttt aatataagaa ataataaaga
    29701 aaattttaga ttggttttaa gtgcaagtgc ctcaaatcct agagtttatc ttacaagtaa
    29761 ttatcaaaaa gaaaaccctc taaaagcgcc tatgttttgt atgttactta gaaaatacat
    29821 acaaggtgga aatatagtag aaatttctca aataggtttt gaaagaataa taaaaataag
    29881 tgttgaatct ttagatgaat taaaagaaaa gacagttaaa aatattatga ttgaaattat
    29941 gggaagacat agcaacataa taataactca tggtgaagaa aataaaatta tagattctat
    30001 aaaaagagtc ccttttagca taagtagagt tcgtcaagtt ttacctggtc atgattattc
    30061 attgccacca gaacaaaaca aactcaaccc tcttgatgat atatctaagg atttgtttat
    30121 taaaaaccta gaggaattgg aaggacctat ctttaagtct atatattcta aatttctagg
    30181 tataagccct attatagcta aagaaatttg ctacagagca ggtgtaaatc aaaatgctat
    30241 cataaaggat atatctgatg aacaatttga ttctttacac aaagtatttt gtaatttatt
    30301 caatgatata aattctaaca aatactctcc ttgcattatt atagataaaa aggtggataa
    30361 ggtagttgat ttcagttgta taaaccttac attatttagc gatttatcat atataaataa
    30421 ggatagtatg tctcgtattc tggaagattt ctatagaact aaggatatta aagatagaat
    30481 taatcaacgt tcatctgatt taaagaagag tatatctgtt aagcttgata gactttataa
    30541 taaacttaaa aaacaagaag aagaattatc agaatccgaa aatgcagaca tttataagat
    30601 aaaaggagaa ttgattactt catatattta tatggtagaa aaaggtatgg aaagtattga
    30661 agttgctaac ttctatgatg aaaattgtaa tgatgtaaca atagaattaa ataaaaactt
    30721 gacaccatct gaaaatgctc aaaaatactt taaaaagtat aacaaaatga aacatgctaa
    30781 agtcgagata tctcatcaaa tttccttaaa taaggaagaa atagattact tagaaaacat
    30841 aattttaagt attgaaaact gtgaaaattt agctgaatta caagatataa aagaagaact
    30901 tgctaaggtt ggatacatca agactcaaaa gaaaaacagt aaaaaggata ctatcccatc
    30961 tacaaaacct catgaatttg tttcttctga tggatttaaa atattggttg gtaaaaacaa
    31021 taagcaaaat gactatctta cactaagact tgctgataat gatgacttat ggatgcatac
    31081 aaagaatatt cctggttctc atgtaataat aaagtgtgct ggtaaggaag tccctgataa
    31141 tactgtattt gaaggagcta tgctagcagc attctttagt aagtcaaaac tttcttctca
    31201 ggtgcctgta gactatacta agagaaaaaa tgtgaaaaaa cctagtggtt ctaaaccagg
    31261 tatggttatt tatgaaacta acagtactat ttatgttact cctgaagaag aaacagtagc
    31321 caagcttaag gttaaatctg aataaattct gcactaaata caaaataaat gttagacaac
    31381 tacacattat cattctaaat aaaaatagtg gccttcttat aaagtacaat aataaggcca
    31441 ctttctaatt agcttacttt taattttcta acaaaatact tttctgtttt aataactata
    31501 cttatattcc caacaaatat tttcagtctt cttatataga tgtaattaaa aagttaacac
    31561 taaaagctat tttttaattt attgatttgt tttagtaatt cattatattc tgattcatac
    31621 aattcttttt ttagattatc tttttctata gataacatag atataacagt tgtaagtcta
    31681 ttttctaata ctagaagatt ttcttttttg atttggtttt ctctactagg agttttttta
    31741 tttctgctaa ttgtatattc gtcataatta caatcaaact cccttatttt agcatctttt
    31801 atctctataa tatagttaca aatatgtgat acaaatactc tatcatgaga cactataagc
    31861 atagttttat cagtatttat cagtgccttt tccagtgctt ccatagattt tatatctaag
    31921 taatttgttg gttcatcaaa tactaaaaaa ttattatctt ccaatattat tttacatagt
    31981 gctattttta ccttttctcc accactaagt acttttactt ttttgtagac atcatcacct
    32041 ttaaacccaa ataggcttaa gtttatcctt ataaaagatt catcaaatga actattaact
    32101 ttagtattgt atagaacact cttttcctca tctaataaac tttgattttg gtcaaaataa
    32161 cctactttta ccttgttatt tatttttata ttgtcatttt tatcagccaa tatttctttt
    32221 atcagagtac tttttccaca cccattatca cctaataatg ctatcttttt acctcttttt
    32281 atcttaaagg aaacattatc taacaggagc ttattttcta actttagagt catatctttt
    32341 acctctacta gatttttact aattatctcc ataccatctt gaatcttaat gttcatttct
    32401 ttagaaactt taggtttatt ctttacctca agtttatcaa ttctgctttt tatagccttc
    32461 acatttgagt ccaacttttt cttattagtc tgaccaccca ttttgtgcaa cctagcttct
    32521 gaattaccca ttctttttgg tgtttttctt atgccatcac ttaaagcttt tttatataaa
    32581 agagcttttt caagcctttt tttctcttta acatattctt gatactcaaa atcagctctt
    32641 tttacttctt catctttttg cattaaataa tcagtgtaat tgcctttgta agcttttagt
    32701 tttccatctt ctagttctaa tatagttgta cataactcat ccagaaaacg tctatcatga
    32761 gatattaata ataatgctcc ctcatgcctt ttaaacatat cttccaaaac cccaatgctt
    32821 tttttatcta agtttgaagt tggctcatca gctattatga tatttttctt attactcagc
    32881 gcatctgcta tttttaactt aaccttttcc cctcctgata agtgttcttc aaatttatct
    32941 ggagcattta aaagactctt ctccttacta tgactacatg tttcaatatt attttcactc
    33001 tgacttatat aagaatagct ttctgtaagg taagtatatc cttcatcaat ttctatttct
    33061 cccaatattg cttttaataa tgttgttttt ccttgaccat ttgaaccaac tagacctatt
    33121 ttgtcatttt ctaatatttc aagtttatct atatccaaaa ttaatttatc tgcataatat
    33181 ttctttaaat tctctacttt tactaacaac ataaaaaatc cctccttaac acacttaaat
    33241 ataaggagag attctataaa taggcataac aaataaacgt atagactata atctactctc
    33301 cttaatttaa gcattgcaaa taaagccacc aaaataggct tttcaaaaat tacaatactt
    33361 taaattataa aaaatagatt atattctcat tgtcctagtt accttgccct ttctttcatt
    33421 tatttttaat cattatatca acattaatta caaatgtcta tagaaaatta actaatttta
    33481 gttattcaat atacaactta ttctttaaat ttattcttct tcaactttaa agtcctgatt
    33541 atttaaaatt ctgtttaata caattccaac aattgcagct aaactaagac cagttatctt
    33601 tactgtactt gttataggga ttccaataga aataccttta ttagataaat atgtggttcc
    33661 aagacctatt aataatataa ctgctatgat taatatattt attttacttt ctacacaaga
    33721 actgtccctt atagttttta atcctacata agttatcatc gaaaatagca ttatactaat
    33781 tccacccata actgaacctg gtatactttg aagaaatcca ccaaactttc ctacacatgc
    33841 aagcaatata gcaaatattg ctgtacgtct aagtattgaa gggtcatagt ttttagttat
    33901 agcaagtact gctgtatttt ctccataagt tgtatttgct ggtcctccta aacaccctgc
    33961 aactatagtc gcaaatccat ccccaagtaa tgttctattt aatccgggat tttctataaa
    34021 gtttttacct actacagcac cattagtagt catatctcct atatgttcca taaatacagc
    34081 taatacaact ggagatataa tcacaattgc tcctaaactg aacttaggaa ctgtaaagtt
    34141 aggtattgca aataaacttg cttctgtaat agtagttata tctactaatc ttaatatcaa
    34201 acagattata tacccagaaa aaactgctat taatattcct aattgtttta taaatcctct
    34261 accaaactta ttggttaaaa ttgctattgc taaagttata gatgctatta caaaattggc
    34321 tgaagccata ctaaaagctg ttggcaataa atttaatcct ataactacta tcatagctcc
    34381 tgtaacttgt gcaggaaaat ataatttaat tttatttaca ccaactattt ttactataat
    34441 cgacattata atataaatta atcctgctac tattatacca ccttgagcat atgctaaatc
    34501 tccaccatat gcctctttag ctgctagtat aactggtata aacgcaaaac ttgaacctaa
    34561 aaaggcaggt acttttcctt ctgtacaaaa atgaaatatc aatgtaccaa caccagcaca
    34621 gaaaattgca actgacggat taaaacctgt caaaatggga actaatactg tcgctccaaa
    34681 catggctaat agatgttgta aagacattat cgtttttttc ataaaaacct ccaaaattat
    34741 tatatataaa taaatcctct gtttaattaa tatcttttaa ttaattatca ttccctagat
    34801 tctatttttt aacctctata tacttattat ataatttatc attattttac attttacgac
    34861 atcaaaaact caaataaagg agaataaaaa ctacattaag actcacatcc ataaaattat
    34921 aatatttatt ttgtgcaatc tatgtaagct ttatcctcct ccctgactat tcaatgctaa
    34981 gcttttatat aatacttaaa aataattatt ctttgattgt aactgaatct tctccatcaa
    35041 tttctaataa actgacagat ataatttcat gtctagaagt aggtacattt tttcctacat
    35101 aatctgctct tataggcaac tctctatgcc ctctgtccac tagcactgct aattgtattg
    35161 atttaggtct tcctatatct ataatagcat ctagtgaagc tctcacagtt cttccagtgt
    35221 ataaaacatc atcaactaaa actactgtct tatcatttac attaaaatct aaataagttc
    35281 ctttaactac aggttcaaca tgaatttttt ccaaatcatc tctatacaaa gttatatcaa
    35341 tatctcctgt atctactttt gttccttcta tttgttctat tttcttagaa attctatttg
    35401 caattggcac acctctagtc tttattccaa ctaaaactag attttctaca cctttatttc
    35461 tttcaattat ttcatgactt attctagtaa ttgctcttgc tattgccttt tcatccatta
    35521 attgagcttt ttctaccata tcataattat tgtcaaagat ataatatatc tatttgacaa
    35581 atctactcct ttcttttaat tttttataaa tttattttta tttaaaattt caatttaaac
    35641 aacaaaaaaa cttctcatca caagacaaga agctcttata taaaaaatat acagtgcaat
    35701 ttagcaattg tcttacgata tctctgtatc gtttaaagat aattttttaa tctatcaaat
    35761 caaattatat atttatattt caaatttttc aagctttttt aaacaaaata ctttaatata
    35821 agatatttta tttaaatttc actaaattta tttacttata taaaaacaaa aaatgattta
    35881 taagtattat acaaatggtt attttatctt tttaataact tccttaaaat actcaggtaa
    35941 ttctgaatta aattcgatat attccttagt agtaggatgt ataaatccca acttcttagc
    36001 gtgcaaagtt tgtcctttta atttaaattt agtttctttt ggaccatata cataatcacc
    36061 taaaagtggg tgatttatag acaaagcatg aactcttatt tgatgagttc taccagtttc
    36121 aagcctagct ctcatatgtg taaacttatc aaatctttct acaacttcaa agtgagtgat
    36181 tgcatttttt ccatctttta ctacagccat ttttaatcta tccttaggat ttctccctat
    36241 aggtttatca acagttattt tatcttcttt aaccacacca tagcatataa attcatactc
    36301 cctagttata gtatgttcct ttaactgttc tgctagaaaa ttatgtgcat aattgttctt
    36361 agctatcata agaagaccag aagtatcttt gtctattcta tgaactatac caggtctaat
    36421 aactccattt attgatgaca acttatcttt acaatgatat aaaatcccgt ttactaacgt
    36481 attttcatag tttccaggag caggatggac aaccatatcc tgaggtttat ttattattaa
    36541 gacatcatca tcttcatata caatatctat tggaatatct tgaggtaaaa cttctaatac
    36601 tttaggttct ggtatctcta taacaatatt atcttcctct ttaaccagat atctagcttt
    36661 ttctacttta ttattaacct ttacttttcc atcttttata attttttgta tataacttct
    36721 tgacatatca cctaattgtt ctgctagata aatatcaagt ctaacttctt cttcctcttc
    36781 aagtactaaa aactctttta cttcatccat tatatcacct acttttactt tcaaaaaata
    36841 aaacatatat acataaaaac acagttccta caactacaaa tacatcagct atattgaata
    36901 catattccca tataattcta aagtcgaagt aatctactac aaaacctaat cgtactctat
    36961 caattagatt tcccaatgca ccagatataa ttaatataat tcccaacctt ccaaatatat
    37021 gtactttcct tgtattaaga tagtatagtc caaatactgt tgcaagtaat gcaacaatta
    37081 taaatatcca ttgattattt tgtaataatc caaatgctgc acctctattt tcgacataag
    37141 ttaaatgaaa tacattattt attattggta ttgttgatac atccaccaaa ttattcaata
    37201 cccatatttt agacagttgg tctaaaccta tgagtagaat tattattaat atatatagca
    37261 atgatttttc cccctataaa ttatttaaaa accaatttta tttgaagcac actattatta
    37321 ttatacaaaa aaaatgctct aaagagcaac tttattaaaa ttgtattttc ttaataaagc
    37381 tagaacaaaa tttaaaatag tactactttt tttatattgt agatttctac atatttaaaa
    37441 tcatatcatt atgtataaaa aatttaaaaa taccatacta atcaatattt tttgtgtatt
    37501 ttatagatta aagattaatg atgtctgtaa tttaaactta caatcaccac taatcttcaa
    37561 acaaatattt ttacttttgc acttgcttat ttttatctaa atgttgtata agttcagata
    37621 ctgttacgaa tttatatcct ctctctttta atttaggaat tactacttct aaagcttgta
    37681 tagtttgtga tttatcattt cttttagtgt tataatcatg taaaagtatt atattaccat
    37741 tttggacctt tgtaaggata gtatttataa ttgcatctac tccagggtta gaccaatctc
    37801 tagggtctaa gtttgaccaa agaattatat tcatgccttt agaaactata atatcacaga
    37861 cacttttact catggctcta tatggaggtc taaatatctt tggttctact cctattacac
    37921 tttttataat attttgcgta tctgttattt ctttttcaac actactataa ctattttttg
    37981 caacatttat atgtgtaaat gtatgattcc ctatttcatg cccctcatca tattgtcttt
    38041 ttaataattc tttgttatat tctacttttt ctccaactac aaaaaaagtt gcttttacat
    38101 catttttctt taatatatca agtacttgtg gtgtaaaatc ctcatctgga ccatcatcaa
    38161 aagtcaatgc taaaattttt tcatcctcat ttcctttttt tattattaca tcttcataat
    38221 cgtcttttat attgtcattt aaactatatg cttcttttga actatcttga ttatgtgtat
    38281 tatgattata tgtatataca cctactatag ataataaaca tatacatata ccccatatta
    38341 ttttcttcat gtcattacct cttttctaat tcattctagc aatatatttt ataatgtcag
    38401 ttttttaatc tctcaatatt ccatttttat ttaaatcagt taaatttgtt aggtcttctg
    38461 aataaaagtt agaatcttct acatcagggt tttgcattgc atcacctaat aaattatttt
    38521 ctttctttgc acaatcatta cataaacttg tatctggcaa tatatccaat ctttctaaat
    38581 ctatttgatt atgacataaa tcacaaatcc catatgttcc attctctatt ttagataatg
    38641 ctaagtctat ttgatacaat cttccctctt catgatttat tagactgttt tgcatatttt
    38701 gcatatataa atctgttccc atgtctccta tatgattatc ataactagat aattctcctg
    38761 atgaatactt ttcacttgta tgtttagttg tatctccaaa taaagtatta tctttcatat
    38821 cctctactaa attagttaaa ttttctcttt ctttcaataa tttatcttta tattttttag
    38881 tatccatttt taacacctct aattttaata tttacatcat aaaaatagta tgtggaaaag
    38941 taaattaaac atacaataaa aataagattt gattcgaaaa atggaatgtc tcaaaatgaa
    39001 atttttagtt catttaaccc tcttttttat atgaaatcaa atatattttt atcctttcag
    39061 caataaaaaa gagacttatc tctattttga gatagtctct tctttgaaca taatttaaat
    39121 acaatttttt aatttttata gttattattt tactaacttg ccaatttatg attattttgt
    39181 tctttaatat gttttgcttt tttaaagaat aaaagaccaa gaattattgc tacaataaca
    39241 cctattccat tagatatatt aactggtagt ctaaatcctt cttttgcttg cattatataa
    39301 ctaaatgtta ctacagtcat aaaaagtgct ggtaatgtag ttatataatg gtttttattt
    39361 gctttgacta aatatgcaga accagtccac aacattatca ttgcaagtgt ttgatttgcc
    39421 caagagaaat atctccaaat tatattaaaa tctataaatg taagagcaac accaactgcg
    39481 aataatggta aagctattaa aaatctattt ttgactgcat cttgattata attcattgca
    39541 tctgctattg tcagcctagc acttctaaat gctgtatctc cagaagttat aggacatgcc
    39601 acaactccaa gtatagctaa agctcctcca ataggcccca ttaatccaac agatactgta
    39661 tttactactg ttgctgctcc accatcatta aatatcacat taagttgagg tatgccatgg
    39721 aaatatgaca tggctgctgc tgcccaaact aaagctatta taccttctgc aaccattgac
    39781 ccataaaata ctggtcttgc ctcactctct ttctcaacac atctcgccat aagtggtgat
    39841 tgagttgcat gaaaacctga tattgctcca catgctattg ttataaataa gtatgggaat
    39901 aaaggtgttc catttggatt catattttgt aaatttatat ttggtatgtt ataccccttt
    39961 attattaatc ctccagctac acctactgcc ataattaata atgctgctcc aaataaagga
    40021 tatattttac caatcaattt atctattggt aaaacagtag ccataatata ataaagtatt
    40081 atcactccaa cccatatttg tgcgtttaca cctgtcaatg tctttaataa tcctgctgga
    40141 cctgttacaa atacaactcc aaccaatact aaaagaataa cactaaatat tctcattgct
    40201 atttttgcac catttcctag ataaaggcct accaactctg atgcagatgt acctttgttt
    40261 ttcattgata gatatcctga agcaaaatcg tgtactccac cagcaaagat tgttccaaac
    40321 acaatccata aaaatgctga tggtccaaat aatgctcctt gtatagctcc aaatatcgga
    40381 cctaaccctg ctatatttag aaactgaatt aaaaagattc tcttccattt cattggcaca
    40441 taatctactc catccctaca agctatagct ggagtttgat ttttgtcatt ggttccaaaa
    40501 accttttcta caaaagtacc atagacaaaa taacctacta taagtactac tattgaacct
    40561 aaaaagctta ccataaattc atccccttag tttttatata attgctttat aaatttttat
    40621 gagtttaaga aaactatttc cagttcaaat agtaatactt ttttatgcta atataaatat
    40681 taattacatt aagtgcatat ttaagtacat gaaatgactt tatatatgca ttaacagcct
    40741 aaattctttt actttgcttc tacttacagt tacttcataa tcactatttt ttaattttaa
    40801 tatgtatgta ttattaaacc atggtataat ttcttttact ttggttaagt ttacgatata
    40861 agacctatgt gttctaaaaa atgttttatc atttataaga ttttcgactt ctgatatgcc
    40921 ttcttttaat ataaactttt ctttctcagt atatataaat gtctgccttt cattagcttc
    40981 acagtaatat atatcatcaa tatctattac aactaattta tcacctttcc aaaggctaat
    41041 tttatgtgtt atttcttcta tctcctgatt tactgcttcc ttcttatatt tgtataaatt
    41101 actatttaca ttaaatagtt caatatcact catctcactt ttttctaatt tattcagcat
    41161 agaaataatt ctctcttcag aatatggttt caatatataa tcaaatacat ccaaagaaaa
    41221 agcatctact gcataacttt catatgctgt tataaaaaca attttaggtc ttgatttaaa
    41281 ctgatttaat gtttttgcaa gtagcattcc atcaagatga ggtatattta tatctaaaaa
    41341 aattacatct attttatttt cctgaataaa gtctaacacc tctataccat tggtaaattc
    41401 acttactacc tcgattccac ttttattttc aataaaatat ctcaattcct ttcttgctgg
    41461 aaattcatct tccactatta tagctctcat acttctactc ctttacataa aatactatca
    41521 aagtcccatt atctattctt cttatattaa gccctcttcc atacatcagt ttaagcctta
    41581 aatgtacatt ataaagccca atcttatttt ctggcatatt atcttgatat acattattta
    41641 taatttcctc acttatacct ataccattat cctctattga tattctcaca gtattttcta
    41701 atgaatacgg taactttttt atggatattt tcactacacc agctctacca ctttctaaaa
    41761 taccatgtat tattgcattt tccactaatg gttgtataat caaacttgga atctttatat
    41821 ttacatcttc tatatcataa gatacagtta gtaattctcc aaacctagct ttttctatct
    41881 ctacaaaaga tttaacttgt tcaatctctt tatttatatc aattaagtta tcactatatt
    41941 caagattata tcttagataa gttgataaat ttattattaa ctctctagcc ttatctggac
    42001 taaatcttat aaatgaagct attgtgttta gggaattaaa caaaaaatgt ggattaattt
    42061 gtctttgaag tgcttttatt tctgctttat ttctcgcttc tatatctttt ttatctttct
    42121 caatcccctc tactatagat ataacaaatc ctattccaat ctgcccaact attaaaggta
    42181 aatatattcc accgattatt tgaattgcta aagaatgtgg atatgatatt aagtatataa
    42241 gtaatatagt aaaactttca gaaatcatgc ctaccaatat tccatacatt actctatgtt
    42301 gttttggtat cctcttatat agaaatcctg ataatacacc tgctagtata cttgataaaa
    42361 gacatggaac agatgttatc cccccaatat ccataaaata tctatgaatc cctgaaaaaa
    42421 ctcctgctgt aatacctacc atcggaccaa ataaaatgcc acctgatact atagatatta
    42481 ttctagtgtt tactattgac cccatataat ttataccact ataagttcca aatatggcta
    42541 aaaaagtaaa aactaatgct atacagacca agtcattaaa actatattct tccttttgaa
    42601 atatctgctt aaatattttt aactttgtta ttagaagaaa caatactaca ataaggcttg
    42661 tcttttctat taatgcgatt aaaattgatg ttttcataat ccccctttaa aaaataaatt
    42721 tttacacact ctaaatttat tatggttgtt ataaattaca tatttgaagt aatcctatca
    42781 caaaactttc cacatttaat cttttaatta aataagaaat agttgtactt ctattaacaa
    42841 ctaatactag ctagcataag cttgtaataa cttgtagtta ttattaactt tacaattcta
    42901 gtatttaatt ttatacttta gaaatctctt ttcttagaaa taatactttc gaatatcata
    42961 taatacaata ttatgtagat tcttactcat acaacatatt tctttaaaaa ttttatacta
    43021 ttctttgttc aaatatctca tttgatttaa ttgtgttaaa cacacattat catctctctg
    43081 ttatatattt attatatagc cgtatatcga taaagtaaat aaatcccata tttaaaataa
    43141 t
//
LOCUS       DAELNY010000016        43093 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.016, whole genome shotgun sequence.
ACCESSION   DAELNY010000016 DAELNY010000000
VERSION     DAELNY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.016"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..1330
                     /locus_tag="KPE55_000730"
     CDS             2..1330
                     /locus_tag="KPE55_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491827.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(1458..2648)
                     /locus_tag="KPE55_000731"
     CDS             complement(1458..2648)
                     /locus_tag="KPE55_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0491828.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2754..4133)
                     /locus_tag="KPE55_000732"
     CDS             complement(2754..4133)
                     /locus_tag="KPE55_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0491829.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4164..5372)
                     /locus_tag="KPE55_000733"
     CDS             complement(4164..5372)
                     /locus_tag="KPE55_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491830.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(6397..7851)
                     /locus_tag="KPE55_000734"
     CDS             complement(6397..7851)
                     /locus_tag="KPE55_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0491831.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(7871..9742)
                     /locus_tag="KPE55_000735"
     CDS             complement(7871..9742)
                     /locus_tag="KPE55_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0491832.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSITLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(9732..10592)
                     /locus_tag="KPE55_000736"
     CDS             complement(9732..10592)
                     /locus_tag="KPE55_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0491833.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGLNLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            complement(11238..11642)
                     /locus_tag="KPE55_000737"
     CDS             complement(11238..11642)
                     /locus_tag="KPE55_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0491834.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            complement(11834..13021)
                     /locus_tag="KPE55_000738"
     CDS             complement(11834..13021)
                     /locus_tag="KPE55_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG0491835.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(13053..13478)
                     /locus_tag="KPE55_000739"
     CDS             complement(13053..13478)
                     /locus_tag="KPE55_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491836.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            13936..14685
                     /locus_tag="KPE55_000740"
     CDS             13936..14685
                     /locus_tag="KPE55_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0491837.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(14851..15216)
                     /locus_tag="KPE55_000741"
     CDS             complement(14851..15216)
                     /locus_tag="KPE55_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0491838.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(15232..16008)
                     /locus_tag="KPE55_000742"
     CDS             complement(15232..16008)
                     /locus_tag="KPE55_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0491839.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(16455..17882)
                     /gene="ascB"
                     /locus_tag="KPE55_000743"
     CDS             complement(16455..17882)
                     /gene="ascB"
                     /locus_tag="KPE55_000743"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0491840.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(17943..19871)
                     /locus_tag="KPE55_000744"
     CDS             complement(17943..19871)
                     /locus_tag="KPE55_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0491841.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(20016..20858)
                     /locus_tag="KPE55_000745"
     CDS             complement(20016..20858)
                     /locus_tag="KPE55_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0491842.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(21205..22569)
                     /locus_tag="KPE55_000746"
     CDS             complement(21205..22569)
                     /locus_tag="KPE55_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0491843.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKKNSILN
                     NIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYFMP
                     IILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYVASVIP
                     ALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLASI
                     INLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCANL
                     AQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGAGI
                     AGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFGVE
                     KNVK"
     gene            complement(22608..23279)
                     /gene="pgmB"
                     /locus_tag="KPE55_000747"
     CDS             complement(22608..23279)
                     /gene="pgmB"
                     /locus_tag="KPE55_000747"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0491844.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNCNTLLQAWSK
                     KINLD"
     gene            complement(23281..25671)
                     /locus_tag="KPE55_000748"
     CDS             complement(23281..25671)
                     /locus_tag="KPE55_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0491845.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPTGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(25697..27145)
                     /locus_tag="KPE55_000749"
     CDS             complement(25697..27145)
                     /locus_tag="KPE55_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0491846.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERYNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(27548..28435)
                     /locus_tag="KPE55_000750"
     CDS             complement(27548..28435)
                     /locus_tag="KPE55_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0491847.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(28535..28984)
                     /locus_tag="KPE55_000751"
     CDS             complement(28535..28984)
                     /locus_tag="KPE55_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0491848.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            complement(28974..30881)
                     /locus_tag="KPE55_000752"
     CDS             complement(28974..30881)
                     /locus_tag="KPE55_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0491849.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(31149..33044)
                     /locus_tag="KPE55_000753"
     CDS             complement(31149..33044)
                     /locus_tag="KPE55_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0491850.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(33080..33958)
                     /locus_tag="KPE55_000754"
     CDS             complement(33080..33958)
                     /locus_tag="KPE55_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG0491851.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(34501..34671)
                     /locus_tag="KPE55_000755"
     CDS             complement(34501..34671)
                     /locus_tag="KPE55_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491852.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            complement(35068..35280)
                     /locus_tag="KPE55_000756"
     CDS             complement(35068..35280)
                     /locus_tag="KPE55_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491853.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(35281..35697)
                     /locus_tag="KPE55_000757"
     CDS             complement(35281..35697)
                     /locus_tag="KPE55_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491854.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(35765..36178)
                     /locus_tag="KPE55_000758"
     CDS             complement(35765..36178)
                     /locus_tag="KPE55_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491855.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(37011..37640)
                     /locus_tag="KPE55_000759"
     CDS             complement(37011..37640)
                     /locus_tag="KPE55_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG0491856.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(37719..38681)
                     /locus_tag="KPE55_000760"
     CDS             complement(37719..38681)
                     /locus_tag="KPE55_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0491857.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(39443..43039)
                     /gene="cbpA"
                     /locus_tag="KPE55_000761"
     CDS             complement(39443..43039)
                     /gene="cbpA"
                     /locus_tag="KPE55_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG0491858.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAGGDTNVGNPEGDANAGNAGGDANAGNAEED
                     ANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLI
                     IDDSVVNRDIEGEFIVSEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQG
                     ILYLWFSDKFTGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEE
                     TMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDN
                     GGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNI
                     NIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKP
                     DINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT        14003 a         7668 c         4574 g        16848 t
ORIGIN      
        1 tatgatgaaa atagcagtca taacaaccga atttttaaaa gagtttgtag ataattctat
       61 aaaaaagtta aatattaatg cagaaattga aatatatatc tatagagatt tttctcatgt
      121 tggagattta tatttagaaa tagaagatag gtttgatgga tttgcagtta gtggccctat
      181 ccccaaaaaa gctattacaa aaaaggctgg aactattaaa aaacctctag ttgattttgg
      241 tactgactta caaagttact atgatatgtt tctaaagctt atctttaaat ataatacatt
      301 agatttttct agagcatact ttgacttaac agactgggca gataatccaa aagatatatc
      361 atattattta tcaaatggca catttggaga cttaatggat agtattgatg aaaatgcatc
      421 taaaatgagt ttatctgaca tagcaaaact tgaaaaaaag attgcaaaaa aacatattaa
      481 tctatggaat gaagggaaaa tcgatttttc tactactcgt tttagtagta ttgttcctat
      541 gttacaaaat gcaggagtaa actttcattt catataccct gatatagatt tattaaaaaa
      601 tacttttaat aacttaataa aagatattca tttacataga atgaagggaa atcaaccaag
      661 tgtaatatat ataacagtta aagatacaaa ttatgatgac tctactctag accaagttta
      721 taaaatatta aatagtatta aaaaagataa attaaccgat tttataattg aaaaaaatag
      781 taattctttt aaaattttca ctaaatgtga aacgattaaa tcattaacta aaaattttac
      841 tgcatgttat ataaaagaat ctattgaaaa tagtacaaat attaaagtat gtgttggata
      901 tggaattggt aatgatataa agcaagctat gtctagtgcc ataagtgcta ataaggaatc
      961 taaaataaat catttaaatt acagtttctt agttaacgaa aattatgaga caataggacc
     1021 tttactgtgt gataagttac ttactgtttc aaataaagta actccacata taaatacaat
     1081 tgccaaaagt tctaaattgt ctactcttac tattcaaaag attatatctg ttactgaaat
     1141 tctacaaaga gatgaagtta ctcctaagga attatcttca tgtttaaatg taacagttag
     1201 aactgctaat agatttctaa gtagtctctt aaagtctgga aacgctacta tattatatga
     1261 aaaacaaaac tcaacaaaag gtagacctga aagggtatac aaattattta tatcagaaaa
     1321 taaatattaa aatccaaaat gcataaaaat attttatcat ctaatattaa gtaaaattat
     1381 atatgacttt ctaaaaggta tatctttgca tctttttaag atataccttt tatgatattt
     1441 aataatattt tatgttatta tctactaaaa ttattttcta accattttac tgcacaatga
     1501 gcaaataaag ctgaaccata aactaatccc tcttcattaa aaattgcttt aggattatgc
     1561 ataccaaaat tatatccctg actaatactt cccattccaa tccataaaaa tactcctgga
     1621 acccttagta aaatctctga aaaatcttct gaacccattg cagatggaaa gtctattaca
     1681 ttttcatttc ctataacttc tttaacataa gatacaatct catctcctac atccaaatca
     1741 tttataactg caggagtccc cattttaaat tccactttag catttcctcc aaatgattta
     1801 gctattcctg tagatatttc aataattcta ttttttaatt tttctctaac aaattggtca
     1861 aatgttctta aagttcctga catagatgct ttatttggta tagcatttgg tacgtttcct
     1921 gcattaaaca taccaatagt taaaactaca ggttctttct gaggtttttc tcttgaaagt
     1981 atagtgttta atgctatttg tatatgcgac attatgttta ttgggtccac tccaaattca
     2041 gggcttgctc catgagttgc ttttccttct actgttattt caaatatatc cgcagatgcc
     2101 atagctggac cacgtgaata agctacttga cctgtgttaa gaaatttacc caccatactg
     2161 tgaaatgcca tagctgcatc gacctgagga ttttctaata taccatcatc aatcattgat
     2221 tttgcacctc tggtagtctc ttcatctggt tcaaatacaa actttactct acctttaagt
     2281 aagtgtttat tatccataag tattttagct gctcctaaga gcattgctgt gtgattatcg
     2341 tgtccacaac tatgagatgc atctttattc ttacttttaa aaggtaaatc cgtctcctcc
     2401 ataataggaa gtgcatccat atctgccctt aataataagg tctttccacc attttcactg
     2461 tctaaaacag ctactattcc attattagac gtcaatttcg gagaatatcc cattttttct
     2521 aaagtactgg taacaaaggt tactgttttc ggtatattgc aaccaatttc gggattttca
     2581 tgtaaatatc ttctccatct aataatttcc ccttctattg actgcgcttc tttaaaaaaa
     2641 tttttcatat tccttcctcc taatcagata gtcagcaaaa tctcactttc ttttagtaat
     2701 gaaatgatgc tgatataaaa attataattt tatatattaa atttcatgtc tttttaagga
     2761 gttggaatat catctggaat aggacattta tatgttttac cattcataga tttatcaaat
     2821 tcttcttttg cctttttagc tatctctgga ttatttaata ttttagctcc aaacatagcc
     2881 ataatctttg atgcatatat catacctttt tctcctatag aacttcctgc acaagatgta
     2941 aactgccagc tatgacctgg tgctcctaga ttcgtacacc ctgtaaaaaa gtatgctgtc
     3001 ggaactatat gcattacatc tccaatatca gtagaaccat aactattaaa gtttgtaaca
     3061 tttcctccac cataatatag atgagttcct tctggcaaat tatatttact tatcatagcc
     3121 ttatattgtt gtgggctttg tctatctaat tcagctgcaa aatccaattc ctctcgagtc
     3181 caaggctctt gtttaatctc attcatacat tctgatacaa gatttgctaa aacatgattg
     3241 tttaaagtat tataacaacc tcctaaaaac tctacttcaa cttcagtttc agtcatcatt
     3301 gcagctcctt ttgctacctt tacaagtctt tcatatgtac tttctacagc ttctctactt
     3361 aaagctcttg tataatacca tactgatgct ttatcaggaa ctatgttggg tgctactcct
     3421 ccatctgtaa ttgtataatg aatccttaca tcagaagtaa catgttctct caaataatta
     3481 gctcccacat tagtaagttc cacagcatct agtgcacttc ttccattttg agggtctgag
     3541 cctgcatgcg cagtctttcc cttaaagtga aactttacag agttaacagc tgttgaagta
     3601 ccaattgtag cttcactaat tttatttgga tgaaaattta tagctaagtc taacccttca
     3661 aacgctccac ctctagccat aaatccttta cctgttaatt gttcttctcc tggacatgca
     3721 taaaatacta tagtcccact taagttattt tctatcatct ctcttttaag ccctactact
     3781 gcccctacat gtgctgtaca taataaatta tgcccacatc catgaccata tgcacccaat
     3841 tcaaatgctt cttgctttgt tgaaactttt tgatttaaac caggtaatgc atcaaattct
     3901 cccataaaac ctataactgg tttacctgaa ccaaaactag ctctaatagc cgtggctaaa
     3961 cctcctacac ctacttccac atcaaatcct tcacttctta gaaagtttgc tgtattttca
     4021 catgctttaa attctttaaa tgctttttct ggattttccc atattttctt acttaaatca
     4081 tcaagtttga atcttttttt atctatctca tttatagata tacttcctat catttcctat
     4141 tcctcctata agtatattta ctatcaacca aatataattg gacctattat actagcaaat
     4201 gccactgatg caatggttac agtaacaaat cctcctataa tcatctttgg taataaataa
     4261 tctgttgccc tttgtttttc ttcttcagtt ccttccatag catcaatacc ttcgttggaa
     4321 attatctgtg ttgcaggata agctagcata caagttacac caattgcagc tgacattctt
     4381 ggagaatatc ccaatagttt tcctacaatt gcagtaaata atattataaa tacagagcct
     4441 acaacaagta ttcctatagt tggaacaatc atcccaaaca atgctgttgg agttacctta
     4501 gcaagtcctc caggtaacat caacatacaa ccaaacatta taattccatt agattgagat
     4561 ttatctaaaa ttcctttttc taaaaaacct attcgtgtaa aaatcaaccc tactaacaaa
     4621 catgctatat taggatttaa ataatagttt gcaggggtaa atcctggtat taaagtagcc
     4681 aatcctacaa aattaccaat catagcaaca aatgaaagct ttgcaagata cactagagta
     4741 gatgattttt tacctaaatc ttctttaaaa attctcatac ttggaagttt aaaattactt
     4801 tttgactcat ctttcaaaaa atccccatta tctcttttaa attttaattc tttatttata
     4861 caatatgtag ttatcggaat gccaaagaac ttttgaaatg ataatactag aattacaaat
     4921 gcagcaagtt caggtctatt tgcagcttct gcaacagttg ttacgataat accagcaaca
     4981 gtagaacccg ctattggagg tgctgctatt aaagcatact ctctaccaaa taacatacta
     5041 cccactgtta aacagccaac ccctattcct acaataccta ctaatgcaat tacaaccgtt
     5101 ttccattcat gacataagtc ttcaagattt ataagtgttc ctaagttagt tattaataaa
     5161 gctataccaa agttgctcat tgcagctaat atccctgatg ctcctgtaat gtcttttggt
     5221 aaaatatttg tccaaaatcc tattaaaaat ataatacata caaataacaa tgttgaaatt
     5281 gcaccttttg ttttcttaga taaaaactca ctaaaagcat aaatcattac acatacagta
     5341 aagcaagtaa gtggattcca aaaattttcc attgttgttt cctcctaaat ataataattt
     5401 tttatcaata aaacaataat tataaaatgt atattatgca gttattctta actacatatg
     5461 gtatagcttg atattttttg atgatattat atgcgtccta aattattcct aaaatatcta
     5521 aataaatata actagagtta actctctaaa tattaactat agttatcaac ttactagatt
     5581 tctttttgat ttttactttc tacataattt tacgagttag aattgtactt caactactct
     5641 ctatattagt atacatgttg gaagacttat atttatttaa atattagata tagaatacat
     5701 ttttgcaaac gtcttaaatt attcttaaaa tatctaaaac gaaataataa tatacccatt
     5761 aaaccaatat tatcagaata tacttcactt taagtcttct ttatatagaa attttatcta
     5821 ttagtgcatc ccctcctttg taaattagac tatttctaaa cgaatttaga tatcttattc
     5881 ctaggaacta ttactagaaa caaaaattat ttattatatt aaatcaatga taagccatac
     5941 tttaaaattc tttctataca agtatgattt atcatataat gacatctata cctttatctt
     6001 tatagtagtc ctaaattatt cttaaattta caataaaaat aatttcacta tttataaaaa
     6061 tacttgtttt tatattgtca aactattctt aatatagatt ataattttaa atcttttctt
     6121 tgtcaatata ttctgacatt tttccagaaa atttataaag ttatatagtg agtaaatcat
     6181 actattttta tataatgagt aaattatact atgaatatgt ctaaatattt acttaagtat
     6241 aaacataaaa aatgtgtatt ctccttttta tagtaatttt agttgccatt acttttaaga
     6301 agaatacaca ctttacttta aagaccctat actctatttt aaatgcttta ctagacacta
     6361 attaacatta gttcctaact tctatttatc ttattcctac cctactacta tatccgtaga
     6421 aaaatctaaa acttctccat tagattttat tacttctttg taccaatcaa atgatttttt
     6481 cttatatctc ttaaaacttc ctttgccttc atcatctaag tcaacataga taaatccata
     6541 tctcttactc atctgacctg tagttgcact gactaaatca attggtcccc acatcaaata
     6601 tccaaaacat tcaacattat cctcttcaat tgcagacttc atttgagata gatgcttact
     6661 taaatattca attctataat catcatttat agttccatct tcttcgattt tatcatatgc
     6721 ccctaaacca ttttctgtaa ttaagatagg taattgatac tttctgtaaa tatagttcaa
     6781 ggtaaatctt aaacctatag ggtctattgc ccacccccac ttactttgtt ccaaatatgg
     6841 attttgaatc ccaccaaata aagatttatc acctgataaa ttttcttttg gagtagatac
     6901 acttgaaaaa taataattaa gtcctataaa atctaactta ccttctttaa atgctttttt
     6961 gtcttcatca gtcacttcaa tagttatatt atttttttca tattctttta atttatattg
     7021 tggaaacttc ccattgcaca ttgcatcaat ttgataataa tcacggtcat tatctaaaaa
     7081 tgcatttaac acattactag gattacaatt gtatggataa attggattta gtccaaatac
     7141 acatccaact ttattgttgg gattgatttc tctagccaac tttgcaacct ttacacccgc
     7201 caaagtcata ttatacccta catttgccaa tgtttgaacc ttattttcta tttcactata
     7261 cttaatacct gctatcatat aagtaaaaat atccgagtgc tctgtttgag ggtcaatatg
     7321 gttcatctca ttaaatgtaa cccagtactt taccttatca tcaaatcttc ttattacagt
     7381 ctcactgtac tttaaataca aatctataag ttttctgtta ttccatgaac catatttatg
     7441 tactaaattc ataggtaatt caaaatgata tagtgttaca ataggttcta tattattttc
     7501 tattaatgta tctatcagac cttcatagta tttaatccct aattcattag gattttcatc
     7561 atctccattt ggaaaaattc ttgaccaatc tatagatatt cttaatgcac taaatcccat
     7621 ttctttaaat aaagctatat cttctttata tctattataa aaatctattc cattatgaga
     7681 tggataatcc aatttttctt ctattttatc agtaattact cttggcgttt cataagagcc
     7741 cttagttaca aaatccataa ttgcaggtcc tttgttatca ctatcccatg aaccttcaca
     7801 ttgatgggct gcaatacttc ctccccagaa aaatgtatct ttaatattca tgaattttcc
     7861 ctcctaaaat ttatactatt acagttaata attcttctga ttcatcattt gatgagatta
     7921 catctaaata atcgctagta tttgtaacta cagttattat agtagaatca aagccttcct
     7981 ctataatttt atcagattca aatactgcta aagcatctcc ttttttaacc ctttgatttt
     8041 gtgttacaag actttcaaag aattttccat ctaagtttac agtatctatc ccaatatgaa
     8101 ttaatatttc taatccttca tcagtcacta taccatatgc atgtttagtt ggaaatacta
     8161 tagttatagt tccatctaca ggagatataa cagttgattt tgattcacta ggtataactc
     8221 caaccccttt acctaatgct ccagatgaaa atgctttgtc attaacttcc ccaagtgcaa
     8281 ttacctgacc ttttgcaaca tttccaatac taatagttct tgaattgttc tcgttgatta
     8341 ttagttctaa ttctttttca ttatcaccct taacttcttc tttttcatct gaaggtattc
     8401 ctagtaaata ggaagctgct gttgctgcta caaatgatac tactacactt attatcataa
     8461 atgtaaagtt agatacctct cctccaacaa gatatgttgg taatgcagct aatccccaaa
     8521 ccattccata tgttttaact tgagctaaac ctgctattaa accacctata cctccaccta
     8581 tcattactgc tacaaatggt cttctatatt tcataaacac accaaacact gaaggttctg
     8641 taactcccat aagtgcacta aaacttactg taccaaataa ttgtttttgt tctttattcc
     8701 tagtttttaa gaaatatcca aacattgcac cacaaactgc aatatcagat attgttgcag
     8761 cgcccataaa aattgggtca taccccaatg ttgtcacaga atttaaagat ataggcatag
     8821 taaagtttgc tgcaccaaag actattaaga gtggttgtaa agctgcatac agtgcaacaa
     8881 cactccaatt acccaatgta tcaattaaaa acatacatgc atagtttaat ccatcaccaa
     8941 tccacatacc aataggtcca aatacagtta atgtaattga tagagttatt attaaagata
     9001 atactggaac taaaaaatag tgtaatgctt taggtaatat ttttttcaaa ccttcaatta
     9061 taattcccat tgcccatact cctaaaagta ttggaataaa actgtttgca taagttgtag
     9121 cctgtaaatt tataccaaat aaacttaatc cttctacacc atctattgat gaagataata
     9181 acgtaactgc taacactact gctatatagg gatttacatt aagtcttttt gctgatgcta
     9241 ttgcaattaa tactggtaag aagtgaaatg tagcacttct taatgtatct aatatttgat
     9301 atgtaggact gtcactctga agtatgccag acatatttaa aagtgctaac atacttgcaa
     9361 gtataccagc tgcaagtagt acttcaatta ctgaagtcat agtctctgag actaccacaa
     9421 agatggaatt aaatatacct ttgatacctc taattccctt ttttctttca ctttttatac
     9481 ttgattcact tgaacttaaa cctatcatag acataaactc gttatataca tctacaacat
     9541 gagtacctat tataatttga taagcaacat cattagaaac tacatcaatt actcctttta
     9601 attttttaat ttcttctgtt tgagcgatac tcctatcatt taaagtaaat cttaaacgag
     9661 taacacaatg cgctaccgac tttatatttt cttttccccc tataagtttt attatatttt
     9721 catttaaatc tttatatttc atctgatttt gcctccagtc tttgagtcac cctatttata
     9781 tgcaacatta aataagtttc ttcacttata gatatatcga cattaaattg tgattttata
     9841 tattttctta tcttttgaac acttccaaat gactttggat aaagttgact tacttgttta
     9901 tataaatcta tagaattatc ttcatctatt gaagcttcat ccgacataat tctttggata
     9961 aagtactgta gatgagtagt aaatctcata taatttgttg aagtttcatc tagctctaca
    10021 ttaaaatgat attttattat agaaactata tctgctaaag ttctcaactc tacaattgta
    10081 ctatcatgag actctttatt tacctccata tttacaaaat gaagtgctat tgatactgcc
    10141 tcatcatcag gaagattaag tcccttacta tcaccaatta attttagtgc atgcattcca
    10201 atttcaaaat gctttggata aaatctttta acattccata ccaaaggact ttttataaat
    10261 tgattctttt cacatctttt aagtaaaaac tctaaatggt ctagtaaggt taagcttaga
    10321 taatcacttg actttatatt taaagctttc tcaccatatt caattatttc attaataaga
    10381 actatattat tggagtctga gttttgtagt agataactaa agtgttctag catatcgtat
    10441 gaatctagta tatatgttct ctctatttca ttttcattta ttacttgtcc cactcttttt
    10501 ttaaagccaa ttcctttaac aaatacaata atttcatttt tacctctttt tactaaggct
    10561 acattattat ttaaaatctg aattattttc atatattcct cccttcaata aaaagaactc
    10621 aagcatatat gtatagagta tccctctatt tttcacatat atacttgagt tctgcctgct
    10681 aatcagtaac atactcaaaa ttatgactac gtcgccataa ttaaaattct tttttgttta
    10741 atttcatatt ctatatgtta tactatccat ataaatctgt caacggtttc ctagatattt
    10801 ttttattcta taactacaag ctctgttatg cgattatctg acaaggttta tatactaaat
    10861 tgatactatt ttaccgtcac taatatttat aatattgtct gaaccattat ctattttact
    10921 attatgaatt atcatagtaa tgcttttaaa tataatgaac tgtatgttaa caaataaatt
    10981 cttaagatat attaacatat aaagtcttaa gtttagtttt atttaatcac tattaagata
    11041 tattaatagg taaagcttca aatttagttt catttaaatg ctagtaagat atactaatat
    11101 ataaatactt aaatttagtt ttatttaaac actagtaaga tatactaata tataaatact
    11161 taaatttagt tttatttaaa cgctctagtg atatttttaa ataaatttta ataaaaatca
    11221 ttctattttg tcataaatca tttatctggc ttatatttaa gatatattgg tggatacttt
    11281 acacttttta attcctcatt acttcttaaa agtttattat tattttccat atcataaatt
    11341 tgtaacatta ctttatttac atatagagca taataattat cactataaaa atattgatac
    11401 tcatatcgag ggtcatcttt gtaatacact ttgtatagta ttccttcgta acttgccttc
    11461 ttggcttttt cttttgtaat actttttata ttatcttttg aaatatcttg ttttaccata
    11521 tacttatcaa tatatgtttc agcatctttc catggtttaa tgcagttttt atatacaaca
    11581 aaccctgtta cacttataat acctataatt aatactataa aaataacttt atattttttc
    11641 atattgatcc tccttcattt attatttaaa actcgatata atgcactaaa aaaaggatgt
    11701 tatctttttt attaaaatat aacatccctc atatttagca tcaatatata tgccccaact
    11761 ggtaggttaa aagaccccac tgtcagtact ttgattaaat tacatgtatg tatatatatt
    11821 tttaacaaat ctattatatg tcaacttgtc tactattaca tataatctgt aatattacat
    11881 ctgctgcttt ttttgagcct cctgcattat aaaagctttt tgatagttta gttgcatttt
    11941 ctttatattc attgtcattt ataacttgaa atactgcttt tttaatactt tctgacttat
    12001 cctcttttaa cataattcct gctcccaaat gaacaactct cttagctacc attctctgct
    12061 ccgaatgttg aggaaataat accataggta ctccataata taaactttca ttaacactat
    12121 tcatcccaga atgagtaaca aacacatcag ttttttgaag tatagcaatc tgttcaacac
    12181 tatttttgac ctcaaaatta tttggtatat ttcctaaact tttaatatca gtacttctac
    12241 caacagacat aatcacatca acatcagaat ctttaaatgc acttatacag tttttataaa
    12301 agctaacatt tttattatta attgtaccaa gcgaaatata tattagcttt ctttttctct
    12361 ctttaggttc aataatcaac tttgatactg atggacctac aaatgaatac ttactagaga
    12421 aggtttctac cattggttga aattcttttg atgtatatac aatggtatcc gtatcattat
    12481 cattagaaac tatagaaata aaatttttta cttcataccc ttttctttgt aaaagcttta
    12541 tttttttatt aatgcgtctt acaccaaaaa tcatacgtat aatttctata aagttttgtt
    12601 tcatcaactt tgctgtatgt ttattaaatg caaaagttgt tgtagagcat acatatggaa
    12661 tattaagttt ttgtgcaaat aactttcccc aaaaacttaa tgaatctgat acaatgcaat
    12721 ctggattaaa atctttaagt tctctacaaa ctttttcatc taaagaaata gtagtatcta
    12781 ctatcatctc aattaatcca ggaaaatcct taccaatttt tttctcatct ccagggagta
    12841 attctggaag gtatttatca caacaaataa attttgctcc agcaccttca attttatctt
    12901 taaattcatt aaaagaataa tacaatacct catttcctct atccactaat tcacgaacta
    12961 cttcaatagt aggattagta tgaccatatg caggaatgct aaaaaatact attttactca
    13021 tatttcatac ctccttttat aattttttta aatcattctt ctttacgtac tactcccaca
    13081 tgacctataa aatttatcaa atcacgttga tgaacaattc ctaatcctct ttcctcatct
    13141 ccaagtacca ataatttatc tcctgcattt atattaaata cttctcttgc ttcttttgga
    13201 atgacaatct gaccacgttc accaactaca actgctccaa agaaaaactt tcctttagga
    13261 ggtactataa tacctgtttt ttcttctgaa tgattaacta agtcatctag tttgacattg
    13321 tacaattttg caagtttaat gcaactacat atatcaggaa tagattctcc actttcccat
    13381 tttgctattg attgtctaga aacattaagt ttttcagcta attcttcttg tgtatactgg
    13441 ttgatttttc ttagactttt taaattctca ttaatcatat ttttcatacc tcctatgttt
    13501 ataatattgg atttattatt taaattctat caactaatct taacattcat atatatattt
    13561 atatactttt attatataaa tttattattc aggtaacata tttccttaag ttatcgtaaa
    13621 caaaaaaaca agcacaaatc tactaactgt tgttaaagta tttaagttaa ggtcatttac
    13681 attatcaaat atttctacac ctttaaaata acctttatat ttataatctt tcataagcaa
    13741 attattatcc cttctcatat acaagtccag ttgatgtctt gttgctaaat ttaatggtaa
    13801 tcaaattctt taccaataag tataaaaatt actaatgtgt tataattttc ttaactacta
    13861 aattttacat acatttgaat agaatatatc aataaattac tataataaat aaaaaatagt
    13921 tagggagata ttattatgat tttatatttt agcggaactg gtaatagccg atatgtggct
    13981 cgtaaaattg cacaagaatt aaatgatgaa ctgatatcac taaatcaact tataaaagag
    14041 gaaaaaacag atgaattaat atcagcagat aaaccattca tatttgtatg tcctacttat
    14101 gcatggagat tgccaatagt agttacggat tttataaaag aaacaaaatt tctaggaagc
    14161 aacagagttt actttgtaat gacttgtggt ggagatacag caaaatccat aaactatatt
    14221 caaaaactat gtaaatacaa ggaatggcaa ttaaaaggta tggcagaaat taaaatgcca
    14281 gagaattata tagctttatt ttctacaaca gacaaagaaa catcaaaaca aatgattaaa
    14341 gaagcagata aacaaatata taggattatt tctgatatac gcaatgaaaa tgaatttgaa
    14401 actattactc ctagtggctt aggaggtaca attaaaagtg gtataataaa tacagttttt
    14461 tataagacaa ttattagtgc caaaggattt cattatactg ataaatgcat tggctgtgga
    14521 aaatgtgtag agctatgtcc tttaaataat attaatttaa aaaacaaaaa accagtttgg
    14581 aaaaataact gtactcattg tatggcatgt atttgtggtt gccccactga agctattgag
    14641 tataaaaata aaactcaaaa cagagaaaga tactatttag aataatatat tcttaatatt
    14701 taaaccatta taaaatgaac tatacccatt gttttataca gttttacata ttttaaccgt
    14761 ctcctaaaat gggtatagtt caaaagatag tgtctcaaag tgaatttttt agttcctaaa
    14821 tcaatctttt tgtattaaat caagtataat ttaatcacct tttcttatat cattttcact
    14881 aatatgttgt attccaacaa atataagatt tattagctct gttagtgact tccattgtga
    14941 tttttttgaa tcaagataat aataattttt cgttccttct ctacgcaaat taacaatctc
    15001 tgcttctttt aatatcttaa gatgatgaga tacagcagga cgagaaagat gtgttttttt
    15061 tgtgatttct cccactcgta taccattaaa atcactctct aaaagagcaa ttaaaatcaa
    15121 ttgtctagtc tcatcaccca tagcagagat agctttgcga cattctttaa atttagacgt
    15181 tatttcttgc attctttgtt gacattcttt ttccataaaa tccaccctct cttataagta
    15241 tctaacagtt gctacttctt tctgtgcagt acgtctatat ttaacatctg ggtatccaat
    15301 tactaatgtt gtaagaacat gattactacg ttttagacct aaaagctttt ttaattttgg
    15361 cgaattattt gctacatctg aaaaatatcc actaaataaa acacctagac cataagactc
    15421 tgccattaat gccatatttg atgctgcaag tgaaccacta atcttatctt ttgtaattat
    15481 cattattgca ataggagcat gcttaaagaa aaagttatcg tcaatctcaa cttcttttga
    15541 atatttaata gctatatttg caataggttt tatttttcta aaaaatttca ctgcttcgtt
    15601 ttcataaatt gactttttat tgtctaaaac aatatatgat acatcttgtg aatttttagc
    15661 actaggggta tacctacctg cctcaataat ttgctttata atttctgaag aaacttcttt
    15721 atctttaaat ttacgtatac ttcttctaga tttaattgcc attagtaatt catctgaatc
    15781 tagctttggc ttatttgtaa gttctatagg aggttcatca aatcctgtca aggtaatagc
    15841 ttttgtggga catattgctg cacaatgtcc acacattaga caatcttgtt ttttaattac
    15901 tgacttttta ttttctataa ttatattgtt tactggacaa tcatttttac aaagcccaca
    15961 tccaatgcaa agttctttgt caacttctat tatatgttgg tatttcatat tcattcatct
    16021 acctttcgtt tattagttta aactattata acacgtagac attttttata ttcattggta
    16081 aagaatttga ttaccattaa atttttaatt tctctattaa ataaattttt catagaattt
    16141 taaaatatca tagtttatac ataataaaaa agacaagctt aaaacctaag cttgcctttt
    16201 tcatctttta ttacaaagta aattgcactt acttcccata cttcttaaca attttatttt
    16261 ctaaaatttt atttgtgaat catactattg catcaataca atgtaaaata attctatatt
    16321 gattttctat agttaaaaaa gttaaaaatt atacttatta ttaattttta tactcttctt
    16381 atttaataca aagttcttat ttaacataga tttcttgtct aatatagatt tcttatttaa
    16441 catagatttc ttgtctaata taaatcttca ccatttgaag caattacttt cttataccat
    16501 tcaaaacttt tctttttcat tcttcttaga cttccattac cactattgtc tctatctaca
    16561 tatataaatc cataacgctt ttccatttca cctgtaccag cacttactaa atcaataggt
    16621 gcccatgtag tataacctaa taaatcgacc ccatctatag aaactgcatc atgcatagct
    16681 tttatatgtt ctcttagata atcaattctg taatcatctt ctacataacc attttcatca
    16741 gctatatctt tagcacctaa tccattttct acaataaata gtggcttttc atatctatca
    16801 tatacatgat ttagtgtaat tctcaaacct aatgggtcaa ttggccatcc ccattgactg
    16861 tatgaaagat atggattttt agcagatgta aatatgtttc cttcagccat atcatctgca
    16921 ttttcatcgg ttctaacaca acggctattg taatatgaaa atgaaataaa atctactgta
    16981 tatttattca ataattcaat atcaccaggt tccatttctg gtgtaatacc tttttgctcc
    17041 atatacttta acgcatagtt aggatattta cctttagatt gtacatccac aaagaagtaa
    17101 ttttctctat ctaactttgt agcttcccat acatcattag gattacaact ataaggatat
    17161 acacttccag ctgcaaacat acatccaatt aagttatttg aatcaacttc atgtgctatt
    17221 ttagttgcaa ttgcactagc tacaagctca tgatgggcag cttgatattt tatcttttca
    17281 acatcttcac cttcttcaaa gcaaatacca gcacccataa atggagcatg taaaatcata
    17341 tttatttcat tgaaagttag ccagtacttc actaatcctt tatatcttct aaagataaca
    17401 ttgcataatt tctcatagaa acctatcatt tttcgattac gccatgcacc atacttttta
    17461 actaaatgca ttggacaatc aaagtgtgta atagtgacta aaggctctat tttatatttt
    17521 ttacactcct taaatatatt ttcataaaat tgaagtccct tttcatttgg tgtttcttca
    17581 tcaccttttg gaaatattcg actccatgca atgctcattc tatatacttt aaatcccatt
    17641 tcagcaaata actttatatc ttctttataa ttatgataca tatcaatacc agttttagct
    17701 ggataataat gctcatcatc aaaatcaaac atttttagtt tccctgtaat aactgctgtt
    17761 ctatcttttc ctgttggaca tacatctaca tttgccaatc ctctttcgtc ttcattgtat
    17821 ccaccttcac attgattggc cgcagtagct cctccccata aaaaatcatt cttaaaactc
    17881 atagtcaaag cctcctaaaa ttataaccaa agcactagat tatgctaata cctcaatctt
    17941 tactaaaata agactgtaat tagattgtct tctaattcaa tatttttact ttctgtttct
    18001 actacatcta aaaattgtga tgaatttgta ataacaattg gagttacagt actatacccc
    18061 aactctttta ttgcataaac atcaaagttt aatatagttt gaccttttgt aattctatca
    18121 ccttgtttta tacatgcctc aaaacctctt ccctctaatt gaatagtatt taatccaata
    18181 tgaataagca cttcaacacc tgtatcagaa agaatcccta tagcatgtag actaggaaat
    18241 aaggttgtaa ctaccccatc tactggagca actacttttc catcactagg tattattgcc
    18301 accccttgac ctagtacacc agatgcaaat gctgcatcct ctatatcact taattttaat
    18361 acttcacctt ttataggact tgttactatt tctctttcta atttagtaga cttgacttct
    18421 tttatttctt ctttaccctt atttcttact tttttagact cttctacttt ctcatcttta
    18481 tataaaatca tagttaatac aaaagctacg accattgata tagctatacc agaaagagct
    18541 actattaagt tacccattcc cccatctggt tcaatcattg ctggaagttc aaatattccc
    18601 attcctccag ttataaattt tctaagatta aagaatccat agaaagcgcc tccaatacca
    18661 ctagcaatac aacttattat aaatggtttc tttaaaggca ataatattcc ataaattgct
    18721 ggctctgtaa ctccaaatat accagatata aagtttggta ttgccatctc ttttagtttt
    18781 ttatctttag tcttgaaaaa tattgccaac acaactgccg aacttgcaaa tgtacaggca
    18841 aagaatggca tcataacatt atcatatcct aaagtcataa cattgtttat atatacagga
    18901 ataaatcccc agtgcatacc aaaaataaca agaatttgcc aagtaaatcc tactatagca
    18961 cctgccaata gtggactaaa atctcttatt gaaattacaa tttgtgaaat aagtgtagaa
    19021 ccaaatgttg ctatagggcc tataactata agtgcaacag gcactgttat taataatgta
    19081 agcattggca caaagaaaaa cttaactaaa tttggaacac acttattaaa taatttttca
    19141 cacctagaag caaaatacac tataaatata attggtacga ctgttccagt ataatcaatt
    19201 gatacaattg gtagtccaaa gaaatcaata tatactggtg atgcaaacat tgttccttta
    19261 aataaagtgt acatcacatc tgctcctgct gatatactgc taccttgtat agcaggataa
    19321 catactgcag ctcctagtgc taatcccaac attggtttta atccaaactt cttagctgaa
    19381 gtatatccta agaacaaagg aaggaatgta aataatgcat ctcctgctgc atttattacc
    19441 atataaccac cagatacatc actatataat cccattgcta caaataatgc attaaatcct
    19501 ttcaacatac cacaagctgc cattattcct aacactggct gaaaaattcc tgatattata
    19561 tctatacctt tatctagtat attacctgat tttttagttt ctgaacttgt acttgaatca
    19621 tctaaaccaa ttatttcaac tacatcttta tagacagctt caacatgatt tcctataact
    19681 acttgatatt gtcctccact tttcattaca gtaacaatac cttccatgtt ttttaataca
    19741 ttgtcatttg ctatactttc atcttttaaa gtaaaacgta atcttgtaat acagtgtact
    19801 aacccactaa tattttcttt tccaccaaca ttgtttacta tctcttgtgc taattgtttg
    19861 tattttccca tttttctcct ccatatttcg tttaaattaa ttttttgaaa tttgaaggtt
    19921 tggccaactc aatgttacca tccaaccata agtataattt tgttagtatc actcctttca
    19981 atcattctta atatactact gaaaaattat ttcacttaca tatttgagtc tttaactact
    20041 ttagctaaat gtattgttaa atatataagc tcatcatctg ttaatatgta gttatattgt
    20101 tttttaatga aatttttaat tttaactaca cattcaaact ctttattata tttagattta
    20161 ataatgctta gtaattcttt gtctgtctca ccttcatgtg cactatctaa aagcaatctt
    20221 tgcgcaaaaa actttaaatg cattataaat ctataataaa acactgattc ttcatgaaat
    20281 tctatcctaa aatgatacct aactatgcta agtacttctt gtattaactt cgtaatatca
    20341 gatgccaaag tatgaccttc acttaactga gcattcacta tatgaaaggc tataaatcca
    20401 gcctcatctt ctggtaattt tatacctgtt ttgttttcaa taatatctag tgctttcatt
    20461 ccaatttcaa actcttcttt ataaaatctt ttaatttccc aaaggattgc attttttaca
    20521 tttattcctt ctttcatcct ttgaattgca gaatatgtat ggtcacttaa ggatatgtaa
    20581 atactttcat ttagcttctt gtttaacttt atttgtgcgt attctataat ttcacttgat
    20641 aatttcatgt gttctatagg aatattattg agtaattctt gtagtttgtt tgatatttct
    20701 ttatttgata ttctaaatac tttatttact ttagttttat ctattagatt gccttttgat
    20761 ttttgataac caattccttt acccataacg ataacttctt catcattatc atctaatgtg
    20821 attacaacat tattgtttaa tatcttatta attatcatga ttatcctcct tttaagcata
    20881 aaaaaagctt atccataata atgggcatac tccttcctag gatactgcta ttattaatga
    20941 ataagcttag cttgtactta aacaattact acctaattcc aattaatatg ataaaacatc
    21001 agacatattt tgtcaacttt ttatttccat atacttcatc aatcttttat gttgcttttt
    21061 gtcagttcta ataattattc ttattaatat taacttattt ctttgcatca atataaatac
    21121 aaaacaagtt atagtcaata ataataaaaa ttctattaat gtaaatagtt aatattatac
    21181 ttcctctact acattatctg tactttattt aacatttttt tcaactccaa aaacatatgt
    21241 cataataaat ggagcaacca atgctataaa tataactata agtccattta ctatatttga
    21301 aatccccttt tcccctgata atccaagtac tgccattata ctattttgtg gaacatataa
    21361 agtcacatga gtaattcctg caaataaccc tgcgattcct gctcctatag atgctaacat
    21421 agcaggtttt ttaagtttca atgtaactcc ataaagagct ggttcagtta tacccatgat
    21481 agctgatatc catgcagctg atgctaactg tttcatctta ctatctttgc ttcttaatgc
    21541 tacagctaat gttgcgccac cttgcgctaa attagcacaa acttgagata ttaatattag
    21601 cgattcttgt cctgtagttg caaatgctga tatgaatata ggagtcaatg catgatgcat
    21661 ccctgtcata acgataaatg gcattgcagc tgctaataaa caaaccatta taaatcctaa
    21721 ccttccttgt agtaaattta ttatactagc taatccctct cctaagaaat ttcctattgg
    21781 tccaattact attaatgcta ttggcgcact tattaataat actaaagttg gatttaaaat
    21841 tgtttttaac atgctaggtg ttaatttatc aatgaatttt tctatatatg acatcatcca
    21901 tactgttaaa agtgcaggga taacagatgc tacatatata acaccttgaa ttggtactcc
    21961 tataaatgat atagggtccc cacttgatac ccatgcagag aaatttggat gtaaaaacac
    22021 acctactact ataactgcta ttgatgtatt aacattaaat tttttagctg catttattgc
    22081 aagtattata ggcataaagt aaaatgctac atctcctatt atatctaata ctctatatgt
    22141 acttgaattt gatgatagcc aattaaaagt ggtgcaaaga gataatataa cttttatcat
    22201 cccagatgct gtaagggctg gtatcatagg tgtcatacaa ccagttattg tatctattat
    22261 attatttaaa atactatttt ttttattttt tatatcgctt ttaatatctc ctgtggtatt
    22321 tatcccctta tttattattg cattaaatac aacacctacc tcattaccta ttataacctg
    22381 atattgagtt gaagttgagt ttactccaac taccttatct atagatttta atttatctgc
    22441 attgactttc ttttcatctt ttaagatgaa ccttaatcgt gtcatacaat gagttaagtc
    22501 aattatatta tcctttcctc caaggccatc aattatcaat tgagctactt cttcatattt
    22561 attcttcata ccttatcctc tcttttaaaa taatttttaa tataatttta atccaaatta
    22621 atctttttac tccaagcttg taatagtgta ttgcaattta ataagctagt atcttctaca
    22681 atatggtcac attttcctag tacttctttt ttccccacac caattgatgt catattagca
    22741 gcatttatag ctttaatacc tgaataagaa tcttctattc ctacacaatc atattgtttt
    22801 aaatttagaa tcttgctccc ttcaataaaa atatcaggat atggttttcc cctttttaat
    22861 aaagatggat ttactataca atcaaattca tcttttattc ccaaagattc taatatccta
    22921 ggtgcattct tactagcaga tgctattccc aatttcacat tatttttttt aagttctttt
    22981 ataagctcat ttatccctgg taatatatct ttatatgtta atgagtttat taactttata
    23041 taaatgttat ttttttcttc acacaccttt aacttttcct ctttagtata actatctact
    23101 ttattagcag ataacaatat tttatctaat gattcttccc tgcttatgcc ttttaatttt
    23161 tcattaaaag tttcatctat ttctatatct agcgattttg ctatctcatg ccaagcttca
    23221 aaatgatact tagcagtatc tgttataact ccatcaagat caaataagac tgctttcata
    23281 ttaattactc cctttaaaac aattattaac ttctattatg ttctttgata attttatttc
    23341 tttattatca tgtaatatgg ttatatttct acctttgatt aacttataat aagatttata
    23401 ttcttctata tttacagaaa ctaaattatc tctaaataat atattaaatt taatattttt
    23461 ccagtgttta ggtatccttg gttcaaaact taatttatca ttttttattt tcattcctgc
    23521 aaatccatat actaaactca tccatgttcc acccatatta gcagcatgta ttccatcact
    23581 tgtatttttt tgtaggttat taatatccat taatgctgtg tccataaaat agttataagc
    23641 tttttcatga tttccaattt cacttgccat cattccaaat attgaccttg acaaagatga
    23701 atcatgagta gttatttttt catagtattc aaaatctctc ttcttttgtt ctaagctaaa
    23761 ctcatcaggg aaaagcaacg ctgaaagtac agtatctgct tgtttattta cttgatatct
    23821 atatattgtt aatggatgat aatggagaag taatggatat ttttcttttg gtgtattttt
    23881 aaaatcccat actgattttt ctaaaaatga atcgtcttgt ttgcttattt tcttctcttc
    23941 atcatatggt aaatacatat tttgagcggc atttatccag tttttcactt catattcttg
    24001 taatttaact tttctaaata cttcatttga aaataaatta tgcttaaatt tatctaccat
    24061 ctgtactgca tatattaaat tatacttagc cattaaatta gtgtagtaat tattgtttac
    24121 tattgctgta tattcatctg gtcctgttac tccatttata cagaatttat tattttttga
    24181 ttctatataa tctccaaatt caacccaaaa tcttgctgtt tctataagaa tttctaagcc
    24241 attttgcatc ataaattctt catcattagt tgcttcaaaa tacgttctaa taccataagc
    24301 tatatctgca tttatatgaa actgtgctgt tcctgttgga taatatgcac tgcattcatt
    24361 accatttata gttctccatg aatataagca acctttattt attccaagtt ctctagctct
    24421 ttctcttgat gcatctaaga ttgagtatct ataatctaat aaagctctag caatggatgg
    24481 ttgagtatat ataaaaaatg gtaatatata catttctgta tcccagaaat aatgcccctc
    24541 atagccatca cctgtaagac cttttgctga tatattagtc tttccatctc ttcctgctga
    24601 ttgataaata tgaaataaat tggtttttat ccccagttga agagttttat cgccttcaat
    24661 ctctacatca gaggttgtcc aaaactcatt cattttcttt ctctgaatat ttttgacttt
    24721 atcaaatcca atattacatg cttctcttaa aaccaattct aatgatttta aatgtgcttc
    24781 tgattctaaa cattttaacg actccagaca ttctaatgat tttaaacgtt cttcatttag
    24841 atttaaatca gaatatactc tactatagcc tacaacaatt tctaaattta acctttcatc
    24901 ttttttggca tagacttcta tttctcgctc aatacattca tctgaaacat tataggtctc
    24961 atttgtaata tttccacaag taatatatga atcaaaccca caccataacc ctaatctact
    25021 tctttctgta actaaattca taaaatactt attgttatat ttttttacat taattttaaa
    25081 ttgcttctta tttatagatg ccactcttgg gtcatctact ttttcacaat taaacttctc
    25141 catactattt tccattttat ttttaaattt tatttttcca ttaaaattca atggtgttat
    25201 tgtatatgaa atcatcatta attcttctat atcttgtgat actaatcttt ctatagatac
    25261 tttaactttt ctaccacaag agttttccca tataaatgaa cgtttaagaa tcccttcatt
    25321 taaatctagt atcctagaat gttcacttgt aatacctttt gataaatcaa aataatctcc
    25381 ttcaatagag aaatttacta tatgagcatt aggtaactta cagatagtct gattttgttt
    25441 tgcatagcca taagcgcttt ctccatatgt aattggagat tcttcataaa atccatttac
    25501 atatgtaccc atattatcta catactcatt attaaaatca gactcttcaa tattccctct
    25561 aactcctaaa tgtccattag ctagtgtaaa cagcgtttca ttggatttta ttgtatcctt
    25621 actaaaccct tgctctataa gttggtcctt agtagtaaat attaaactca taataacact
    25681 cctttcttaa aatgaattat gcatgagttt ccatgtcatt aaataaaatt tctccatctg
    25741 tttctattac ttttttatac caaaaagcac tttgtttttt agttctttta aatgagcctc
    25801 ttccatagtc atcagaatca acatgaatta tgccatatcg ttttgtcatc tttccttcac
    25861 ttacactggt tacatctata catccccaca taagatatcc tataagctct attccatctt
    25921 catttactcc aagaatcata tttttaatat gttctttcat gtaatctata cgataatcat
    25981 caattatttt tccatcttca ttcacaatat cttgagtacc cagtccattt tctactataa
    26041 ataaaggtaa ctgatacctt tcatataact gattcatagt tattcttaac cctacagggt
    26101 cgttttgcca tacactattt gattttggaa gatatggatt ttttatggtt tctggtccgt
    26161 ttgcctttga tttttctaaa ccttcagctc tagctgaact acaagcacta gaataatagc
    26221 taaaagatac aaaatctaca gtattatcct taagtgttga taaatcttcc tcttttatta
    26281 ttaattttat ttgatttgat tttaaataat aattcataaa gcttggatac acacccctaa
    26341 cttgaacatc agaaaaaaac aaattatacc tttcatgctc taaagttgca aatacatctt
    26401 ctggtttaca tgtgtatgga tatgtttttc cagcagctaa catacaccca aacttaaatt
    26461 ctttatttat ataccttcct atttctatag tttttgcatt agctaataat tgatggtgtg
    26521 ctgcttgata ttttttctga agttgatttt catcagctat aaaagttaac ccagcaccta
    26581 tatatggaat atgcattatc atattcattt cgttaaaagg aatccagtat ttgacatcat
    26641 ctttatatct tttcatcaaa atttcagcat attttacata taaattaacc actcttctat
    26701 cagcccaccc agaatatttt tcaactagag aataaggtat atcaaagtgg cttatcgtta
    26761 ttaatggctc tatattatat ttttttaatt cttttattat attatcataa agttgtaatc
    26821 ccttttcatt tggttcactt tcttctcctg ttgggaatat tcttgaccaa gatatagaaa
    26881 atctaaaaca tttaaaacct atttctgcta acattgctat atcttctttg taattttcac
    26941 aaaatttaat ccctatatga cttggatagt attgattatt ttcatttaaa atattatcct
    27001 catctaaaca tacatctttt aatctcttat catttaatgg gagtttatca aactgagata
    27061 aaccttttcc tccatctaaa taaaatcctt cactttgatt ggctgaaatt gcccctcccc
    27121 ataaaaaatc tttccctaat ttcatgcatc tacctctttc acaatataaa gttttataat
    27181 ttctatctca tatttagtta taagattttt ttcatactat accttttaaa taattcaatt
    27241 taaaaatcca attatatttt ttactcacta taattaatat tgtctatttt caatactttc
    27301 atattaaata tcaatattag atatctatgt atataattat atatatagca aatacatttt
    27361 tatatcctca ttattgttat cttattctca tatattgctg tatatttata tataaaagga
    27421 tttatttcga aatagaggtt gtattgagaa ctaaaattta gactttataa atgaatggaa
    27481 ttttcattgt tgaaatactg aaaatatatt tgatttcata taaaagaaag tgcctcatga
    27541 actaagttca ttttgagaca ctcccttttt tttccaagct attatatctt tttttaaatt
    27601 ctaatggaga gactccaatt ttttctttaa atactctgta aaaagttttt gtacttccaa
    27661 aaccaacttc ttcaaccaaa tctacaatgg atatattgtt attaagtaat atccactttg
    27721 ctttttctaa tctatattca tttaaaaaag ttataaatgt ttttcctgta ttttttttga
    27781 aaaatttagt aaaataatat acactaaatc ctacttcata agacacatct tctaatttta
    27841 taggttgttt ataatttttt tctacatatt tgaatatatt gttcaatctc tctaatatct
    27901 ctttagagtt tatttcatat tctactgtat tttcttcaat ttctctagga atctgtctat
    27961 ataatatagg aattaaatta tacattttac cttttattgc ataactatat ccttctattt
    28021 tgtataggtc ttcattgtag atatctatca atatatcgaa aactgacttt cttatttttg
    28081 ctgaccaatc tctactacat ttttttatac tactgaataa attaattaag ctttcttttt
    28141 cctcatttaa taaaattaaa tcattaaaaa aagatatatc aaattgaaat actaatcttt
    28201 cacttcccgg tgatggcagt atataatgaa tatctcctcc ttttattatc actatctcgc
    28261 ctttttttaa ttgcataggc ttgtcattta taaatagatt tactacacct tctgttataa
    28321 gtaatatttc tatttcctta tgccagtgtg gtgttgttaa tacttctcct tcatttgata
    28381 gactacgaaa aggaaattca tgtattaatt taggcatttc taaatatatt gccatatata
    28441 tcctctcctc tctagtgtct aaaaattaat ttatactatt taaaagaata cacattttat
    28501 tttaaaaacc tcaaatttat aatttataca atacctacac ttcataaaat atatctaata
    28561 tttctttttt tgtatttgca tttaaaagtc tttcacctat atttttacca cctaaaatgt
    28621 aagctatttt agatattaat atcatatctt cattgcctcc agattgtggc atagcaaaca
    28681 taattattac ctttacaggt aagccatcta tacttggcca atctacacct tttttagatt
    28741 tacctattac tatggatgcc ttctttacat attttgtttt tccatgtggc atggccattt
    28801 gataacctat gctagtagag cctatttttt ctctatgttt taatgccttt ataaacttac
    28861 tttttaaagt tatacttcct gtatcgtata atttatctac taattcctgt attatgcctt
    28921 cttttgtagt tgatttcaaa tctaaaataa tgctttcttc tgttaatatt tctctaattt
    28981 ccatttgttt tctccaatag attattctta aataatattt taatatcctc tttgctcttg
    29041 caatctatta tatagtttat atagttcttt tgcctatata aatttgatat ttccattaaa
    29101 atattcttta aaaaactttg attactaatt tctaaataaa tatcaatcgt tctagtaaca
    29161 ttattgatat tcattatccc tttatttaat ttactttgct tatgtatata tatatttaaa
    29221 tcttttccta atgaaataga ttcatattta ctccctaaat tcacacctat taaactttcc
    29281 atctgagcta catcaaaatt aaaatatata ttatcttcta aatacattcc taataagttt
    29341 gtatctaagt tcatactttt tttaggttta gaaagtaaag cattttttat attattaata
    29401 tcattttcca ttaatagtac tgatacaact atatgtaatt tatctttcaa atctaagttt
    29461 acagtagata ttacaaagtc tacatcatct aaatttcgct tactcaactg atgcatagga
    29521 actatatcaa ctatagtcca ttcaggaaat tctcttttta atctggctgc aaggagctga
    29581 gatgttccat atcctgtatg acaaactact attactcttt tactactcat atttctttct
    29641 attgccgctt gaaaatatgt ggcaagatac gaaacttcat ctatactaat attcttcaga
    29701 ttatcatact ctgtcataat atttatagtc atcatacata atcccagcac atctgaaaat
    29761 cgctcttgta tctctcctaa caacgggttt tttatttgga tatcatatct taatctgttt
    29821 aacataggcc ttacatgaga tgctagactt ttctgtagta agtcatcatt tttgagattt
    29881 atatttaaaa atcgtgacat attctctatc attatagatg aaactttttc actttcactt
    29941 aatctatctg attcatcatt gtaactacta ttattttcgc tactaaatcc agatgatacc
    30001 aaaaaaatat atatgtacat aatttcatct ttcgttattt ttactctgta tctattttct
    30061 attttatcta taagtgtact gacattttta tatactaact cgtccaaatt atctacagaa
    30121 atttctcttt cttcctgaga ttctatttga ttaccttctt cgattctctt taaacatata
    30181 agtatatggg taattaaatt tatatagtag ggctgattta tagtatatcc taaattgtat
    30241 tctaattctg taattatagt ttctacaaat ataatagaat caatatcaaa taaatttatc
    30301 aatgcactaa atgtagcaga atctaatctt gttaactcag atatatcatg ttgttcataa
    30361 ttttcatcta ataaatcatc catcattttg gcaatcgatt tccttatatc cacttcttta
    30421 cctataatct tagttccttc taaagtttta ttaagtttta aattgtattc ttttatccat
    30481 gcctctatat atttaaaatc atttactata gaagttttac ttacataata tttatctgct
    30541 aactgattta ttgaagttat attatttgaa tttagtaata aatactttaa aatctccatt
    30601 cttctctgct ctactgataa agatttattt tccttcgatt gtatatttaa actattaatt
    30661 aagtctatat tcttctttgc taaatcttct attaatattc catagcctct ttttatatca
    30721 atttttatat taaaagtttc taaatatttc tcaatcatct tcaaatcttt ttgtaaagtt
    30781 ttatcagata catttaattt atctttaaag tattttatag gtctatattt attttcgttt
    30841 agtatcaatt taagaaattc aatttgcctc gacacaagca tacttagccc tccctaccta
    30901 tgtttatata tttctatgtt tatattaagt actattttaa catatttact aactatgtaa
    30961 aattactaag atacttctgt atcataaaag aatttatgat acaccttact aaattgatat
    31021 aatagataat ataatttcaa aataaaaatt aaggatattt agaagtagtt aactgattct
    31081 aaatatcctt attattattc tatatacttg ttgttattcc attaattatt tttactatta
    31141 tttataattt aagcaccaag taattcaact tcactatttt cactccttat agtaggcttt
    31201 gttaccccta taatcaatgc tgttattact gtaccaatca atatacaagc tatatatgca
    31261 actggtgcac tacatgcaaa tggtattaca aatattcccc catgtggtgc cattaaagtt
    31321 atatctaaac tcatacatat tgccccacct atacaagaac caattatatt tgctggaatc
    31381 attcttaatg ggtctgcagc tgcaaacggt attgcgcctt ctgttatgaa agatgccccc
    31441 attatagcag ttggtagaga tgactttctt tcttctttag taaacttatt tttgaatatt
    31501 aacatagcta atgatattgc taatggaggt accattcctc ctaccattaa tgctgctgat
    31561 ggttcaaata tattacttga aaacatagcc aatccaaaag ctgaagctgt tttattaata
    31621 ggtcctccca tatctgatgc catcatccct gctaatattg cacctaataa tactttatta
    31681 gttccagaca tactttgtaa ccaagattgt aaagcttcat ttaaccatga tgctggcgta
    31741 ttaactacaa agaacattat agctccactc gtaaatgcaa tcaaaagtgg acatatcaat
    31801 acagcattta ctccttttaa tacatctggt aaattttctg ttatcttcat tactcctaaa
    31861 gctatatatc ctgttgcaaa accagctacc ataccaccta taaaacctga acctccaata
    31921 actgccataa aaccagctat catagcagga gcaaatcctt gtcttcctgc tattgattta
    31981 gctataaaac cagcaagtat cggtataaat aacttaaatg ctgaatcgcc tccaagttgt
    32041 ccaaagaatc cagctatagg gttgtatgtt tcatgagttg ggtctgatgc aaatatacca
    32101 aacataaaag ctatggctat accaattcca cctatcacta caaatggaat catttcagat
    32161 acaccactca ttaagtgctt ataaacaccc tttttctctg acccttgaag catttctttt
    32221 gaatcttcta aagtctttcc acctggttta atttctttag ccattccatt ttcagcgttt
    32281 ataattaaat ttttagctaa tgaaacattc ttagcatcta ctactttctt ttgtagcact
    32341 ttctttccta taaatctact cagttgaact tctttatcac aagctattat tacgtattca
    32401 gctcttttta tatcttcttt agtcaactta ttttctacac ctatagctcc atgagtttct
    32461 attttaactt catatccaag ttcttgacct gcttcctcta atgctttttt tgccataaaa
    32521 gtatgagcta tacctgttgg acatcctgtt atcccaacta taagaccttt attttgatta
    32581 ttactaaaat ctcctgattc tgcagcttta ttattaaata tacttaatat ttcgtatgga
    32641 gtttctgcac ttagtagatg cttaagaaaa ttttcttcca ttagatatgt agctagttta
    32701 gatataatct taagatgttc atctgaacct gattctggaa ctgctatcat aaaaaatgac
    32761 ctaagttttt gaccatcatt atctgtaatc tctctatctg ttcttaaata catcattaca
    32821 ggttgattta ctacatttga cttagcatga ggtatagcta ttaaattacc catattagtt
    32881 gatgattctt tttcgcgttt attaatatca gctataaact tttctttatt atttatataa
    32941 tttccatggt acaaatgatt tgccatataa ttaattacat ctttttttgt ttgtaattgt
    33001 ttatctagta caatacaatc gcttcgtagt attttaccta tcataaattc tcccctcctt
    33061 ttaatttgtt acctcataat taatttttat ttttataaaa gcactttaac ttatctatag
    33121 cattgaatag attcatttta aactctataa cttctcttgc agattttata catggtggat
    33181 atatagtatc tggctcatat aagcttgggt tttgttctaa tagttctctg cacttcttat
    33241 aatatgccga cttaacatca ctcgaaatat ttattttaca aactcctagt ttaactgctt
    33301 gtgctatttc ttcatctgga tttgatgaac caccatgaag aactaaaggt atatctacta
    33361 cttctcttat ttcttttaat aaatctagtt taagctctgg tttaaaacct tttggatata
    33421 taccatgtgc tgtaccaatt gcaatagcta gtgtttctac tcctgttttt tcaacaaaat
    33481 ccttagctaa ttttgaatct gtatatatta tttcatcagt accaccttca cttgatgttc
    33541 ctgttgttcc tattgttcca agttctcctt ctacggacac atttaatcct tttgctattt
    33601 ctactatttc cctagatata gctacattct cttcatatgg taaatgtgag ccatcaatca
    33661 ttactgatgt aaatccacat cttattgccc ttaatatttg ttctttattt tccccatgat
    33721 ctaagtgtat taccataggt actttactct tatttgcttc ttctatgcaa ctagctacaa
    33781 agctatctcc tagaaaactt agctcatttg ggtgtattgc aagtattact ggtgcatttt
    33841 tttcgttagc actctgtact actgctttaa gtatttgccc acttcctata ttaaatgctg
    33901 gtacagcaaa ttgattttct tgtgccactt ttagcatttc cttcatgttt attaacatat
    33961 tgtacctccc gatattttaa attttaggtt gaatttgctt cttgttaatt ttgttgttgt
    34021 ttatatttaa ataatatcag ggtaatgaat gtcagaaaat aacaatcgtt ttccttagtg
    34081 aaaaggaact tttattatac actctaagtt ccttttcact aaataatttt tagaagtctg
    34141 catcatagtt aattatattt ctcatgtaca tcttctcttt ttaagtgttt tatgctttag
    34201 ccttcctaga ttttataata aacaattaag atacattaac tataaattta aagatactca
    34261 tgcaatagtt aatgctatat tagagtcaaa aaaattaaaa agatattagc tatttggcca
    34321 ataccttttc ttggattcca atagtatata cgatttatag aaatcttact cacgattatc
    34381 taatgtttta cttccataaa ctttacataa aacaccaaag tgacaccctc caaattcatc
    34441 tctagctaat aaagtgattt ggttttctaa gcaccaattt ataaattagc ctttgttatc
    34501 ttatgtaaat ctttttctat ctaatttaaa aattattcct aatgctatat agcatatacc
    34561 aattactaat acaccataag ccatagatac tcctatctta aattcttgca tattaattac
    34621 aatagaaact gaaatagatt tattacaaac accataaagc aaggctgaca ttgtatatta
    34681 tccaattgta tgaatgaata ctaatatagc tccttttaat taaatgatgc tatataaatc
    34741 atatttatcc caatttttaa tctaacctat ttatatgtat attccttttt aataaaaaaa
    34801 gatattgact aaatagtcaa tatcttttag ttgatttaaa tacccacaat tttaatctaa
    34861 tttttaagtg tttccttaac ataatcttat aaatattaaa atttatctac ctataaaatt
    34921 tctccattaa attttattac attttatctt aaaacaatta taatctgacc ttaatcatat
    34981 attatattat ataaatattt ttaacaaact taataattaa ataactatat tttattatcc
    35041 atcttataaa tgtttataaa ctttccacta ttcttcttca ataaattcaa acaaattttc
    35101 tactgtagta tcgaatactt tagcgatatc catagctaac ttcaaagaag gattatattt
    35161 cccattttct aagtgtacaa tagtctcacg tcttacccca accaatttag caagttcatt
    35221 ttgcgtcata aggagttttt ctctgtattc tttaattttt gttttcatca atattgccac
    35281 ttagttacat atcccccttt tatcaaagta tatatacaac actaatttag ataaagactg
    35341 tataaataaa actgcaatga ggattatacc tgtatctagg tttgatatat ttatattact
    35401 tgtataaggt gttgcaaaga aaatagatat tattactata gaatatatca ttatttttat
    35461 tgcaatctta ttaacctttg atagtataaa ttcagatgat tcatcaacta aatcattata
    35521 ttttttatta taaattgaat aacatccaaa aactattaac caactcaacc ctggaagaga
    35581 agcagtgctt ggacctttaa aaatatactt agtggctaat acagctataa aaaaataagt
    35641 tattacaagc aactccagga taagctttga tcttacacca gtttctgatt tagacatata
    35701 aaatcactcc ttaaaaatta tattctctat actctttaat tttcgttttt atcaatatta
    35761 tcacttagtt gcatattccc tttctatcaa aatgtatata caaaattaat ttagataaag
    35821 attgtataaa taaaatagct aagagaatta cacctatatc caaatttgat atatccaaac
    35881 ctcttgtata atttgctgaa aagaaaacag ctattagcaa aatagaatat aacattattt
    35941 ttgatgcaat atgatcaacc tttaataaca taagttttga cgcctcgtca actaaatcat
    36001 tatatttttt attatacatg aagtcacatg cccaaaaagc gaataaaatt ataaaagtaa
    36061 tacaaagatt tccctcttta aaaataaact tttttaataa tgagtctata aaaaaatagg
    36121 atatcataag taactctgaa acaagatttg ttttaacacc aagctctgat ttagacatat
    36181 aaaatcaccc cttaaaagtt atatatttat aacaccttat gttataaata tataactttt
    36241 ccaaataaat tgcaatagaa tattgaaaat attcaataat atcttcataa atatatttta
    36301 taaaagattt tataataaac attacaatac agtactaaat tagtttattt catctatcat
    36361 ctcaatcata tcatttacta actattattc taatgagcat ataacattga aacttataat
    36421 tatacaacct aaatgcctcc caatgtaccc tcaaaacatc atttcctgta actgaagtat
    36481 aattggtata ttttatcact gaaataaaaa aacactagtc aataaagact agtattttca
    36541 agtattttat cattcttttt ttaattattg gagacgaatc atcagaataa tgcccataat
    36601 ttatctatat gcaaattcta aatttaaaat aaagtttcag ataaatttac tattctatgc
    36661 tatgatacaa cagaaaaaac tgaaatctat gagtggtagc ttcatataat cctagttcct
    36721 acagtaatca aaactgaggt ttattctata tgtaactaat gataaatttt attataggag
    36781 ttattagatc tatttgtgtt actataagta gtaattatat atatgataat tttttaacac
    36841 ttttctgttg gctatataga aagtggttta gaatttaaat ttaaataaaa tttaaattca
    36901 ccaaaaagaa atctatatta gaaaccacta ctctattcca ccagattgta attttttgtt
    36961 ctttatatca caatgctata ccatattaaa ttgaaaaata agttttaaat ttatttgtta
    37021 caattcatta attcaaaaaa atattttgag tcatgagttt tgtaaagatt tagccatgaa
    37081 tttaatgctt ctgattcaaa aacatctaaa agtttcaata cttgaaacgt aaattccaac
    37141 ggagcaattc ctgaagccgt aactaaattt tcatcactaa ctacagattc cgttttataa
    37201 tatttttctc cagagtaatt tggtattacc attttaagaa actctaagtc attacttgta
    37261 tgatttctaa aatctaatag ccctttttta gctaggccta atgtagctcc acatatggct
    37321 gcaactatag aatcttgtct taaagcttct ttaacttttt ctaatattat ctcatgattc
    37381 atagataacc aagtatttcc ccctggcaaa attaataaat ccttactttc catattaaac
    37441 tcatcaactg taatttcagg taaaactttt aacccaccca ttgttataat aggattttta
    37501 tcaattccta cagtaattac ttttagtggc tttaatcctt ttttaaaata tcttcctgta
    37561 tttaattcag cagttaaata tgccacttcc caatctgaca tagtatcaaa tacatacata
    37621 tatacttttc tatttttcat cttttttact ccttatgtaa ttaatttata tatctaatta
    37681 taaaataact taactgacat attcagtcag ttaagctatt aaatttgata ataatcaatt
    37741 aattctagta aatcttcaat tattttcttc ctaaggctta gtggttcaat tacttgtatt
    37801 gtctttctat aaggtaaaag tatatttggt atgtacttgc atattacttc ctcttcaagc
    37861 ataaatatta ttttattagg tgttctttct ttcaagtagt gccctaaaaa ccaatgttgg
    37921 cttaaactat ctaaagaatc tatactccct tctataacta attgaattat attttgttca
    37981 tcttttattt caggtataat tccttttata aaaaattcac ttgctgaaaa ttctgtcggt
    38041 ctgttaaatg tatttgatgt ttctatgatt tcactaatcc tatccactct gaaactacga
    38101 acctcatttc tcatattaca aaatgcaact acgtaccaat tattattcca gtgaacaatt
    38161 ccatatggat ttataattct atattttgat tttactttac gacctgcatg atagttgatt
    38221 tctacagata gctcctttaa tatactattc tccaattttt taagtactga ttctatagac
    38281 actttcccaa ttggttttat tacttcaagg gctcttaagt ggtttgttaa aatatcttct
    38341 tgtttttgat ttgaatacat ttttaacttt aatatcgatt tatctaatgc ttcgtctaag
    38401 aagtatcctt cttttttagc aaatagtgct gcatgaagta gagatttttg ctcttctata
    38461 tcaaaaaaaa gaggtgcttt agtaaaatta tttaaaaggc tatacccacc attatgacct
    38521 gtatctgaaa ttataggtac tccactcgca gaaagtacat caatatatcg gtacactgtt
    38581 cttatattta tttctaattt ttcagctatc tgttttgctg taattttttt gtcagagttt
    38641 aacatccaca gtatagacat catattgtca attttagcca tgtagtttca cctctttcta
    38701 tttgttatat aaagttatta tataactttt tgattgattt tttacaaaaa aacattagta
    38761 aactgctaat aatttatact aagatttatt tcaaatattt atagatagtc tttttctata
    38821 gttaagttat ccattaaatt acatagtagt atatctattt tagttgatat attttcagct
    38881 acctcccttg aatattatta caataagtct aaatcttctt tcttccataa atatattatg
    38941 aggaaagact atcatcttta atggtttaat agtataaaag atattaattt aaaaaaatct
    39001 atttttatat cagatgggta ttgtttttta taaaactgaa aaaatcacct aatagaatct
    39061 atgatttttt aacataacct ccataaatta gttccaatta tcacttcaaa ttcaaatttt
    39121 tatctgaaga atatacaatc atatttccat actcaacata atattgagtg tttggcttgt
    39181 atttacaaat tattattcta acaaaaacaa ctatacatag attaaaatca tcttctaaac
    39241 ataactacta aaattgaatt aatgatatca attccattaa tctcttttaa taaaaaattc
    39301 tcttttctat atgaaaatta tttcaaaact tttaagttaa atacaaaaat gactataaat
    39361 agatttttat ttacacaaat ctatttatag tcactatagt ttatccaata aaataataaa
    39421 ataaatatgt acacaataaa tgttatttac gtcttaagta tttatttgtt agatttttaa
    39481 ttaaaaaaaa caagctaatg aacattaatg ctaaatctag cattatattg ctttgatctc
    39541 ctgtttgtac attttgacct acattactat ctaaactttc atctgtatct atatttatat
    39601 ctggttttcc attcccatct gtatctatat ttatatccgg ttttccatct ccatctatat
    39661 ctatatttat atctggtttt ccatctccat ctgtatctat atttatatct ggttttccat
    39721 ctccatctgt gtctatattt atatctggtt ttccatctcc atctgtgtct atatttatat
    39781 tcggttttcc atccccatct gtatctatat ctggtttgct atttcctcca ttatctgggt
    39841 ctggtttact atttccaccg ttatctggat ccggtttgct atttcctcca ttatctggat
    39901 ctggtttacc attgccaccg ttatctggat ctggtttgct atttcctccg ttatctggat
    39961 ccggtttgct atttcctcca ttacctgaat ccggtttgct atttcctcca ttacctgaat
    40021 ccggtttgct atttcctcca ttacctgaat ctggtttgct atttcctcca ttacctgaat
    40081 ctggtttgct atttcctcca ttatctggat ctggcttatt tagatttgga ttacctgtat
    40141 cataacctcc tgagccagta tctacatttt tatcaggctt ccaataatca tctactccat
    40201 cattatctgt atctattgct ggacgattat aatctgtatc agggtttcca tctccattgg
    40261 tatctatatt tttatcaggt ttccaaatac catctccatt tccaccttct gtactaggct
    40321 tccattcacc tgtattatca gtatctatgt taatatctgg cttccaatca ccactcgtat
    40381 ctatattgat atctggcctt ccatcaccat cagtgtctat atttatatcc ggtattccat
    40441 ccccatctac atctacattt gtcatatcta actttaactt cttttcatcc tcactacctt
    40501 caatgtaatt tatatcatct ccaatatatt ttacagtaat agtttgttca gtagtaaaaa
    40561 tatgatgatt gctatctaaa ggtctccatt caatagatgc agtactataa ttatgactct
    40621 cactaccctt attatgagtt tttaattcta ctggatcttt atattccttt ccattaatat
    40681 agaattgtac gtaacctctt ggagaggcac aatctggtcc ttctttctta tctggtgaaa
    40741 tagtaacatt taatgtaaac gtatctgttg gtttaaaaac atcagtttta gctttagact
    40801 cttctacaac taatgaagtt tctgtatcag caggaataat ttctgcctta taatatgctc
    40861 tatgccccca ataagcatcc ttaaaaggat atatactcga aaactgtgtt gatgtaatgc
    40921 ttaattgata tttcccagca tcaacttgtt ttgtaagatc atcacttcct aaatttatat
    40981 ctggttccat cgtttcctca tttaaacgtt gagaactaat agtcataaat tttttatctg
    41041 tgagtttttc acctttagga gtagttattg gaataccacc atcttttaat agccaatcta
    41101 aaaatgattt ttctacgtct tcttcaaatg gtaatccatc atatctttta ataatcaaat
    41161 atggtggagt atcatcaata gtaaattctt catattgctt taaaaattga gaacctgaac
    41221 ctatatcttt aatataaaaa tcatctggtt ttagaacttc tccagtaatt ttatctataa
    41281 caactaaatt aacagatact tcagatccag taaatttatc agagaaccat aagtaaagaa
    41341 tcccttgagc atctgtataa ctaggtgctc cataaggata aatttttcca tcaattagca
    41401 ttttaaaatc atcaactagt gtagtactaa ctttatcttg ccctaaatac gttagagata
    41461 ttttattcat aaaaactttt gttttctttt ctaaatcacc gtatgcaact gctccaccta
    41521 aacttttgaa tgttgttgct tttaatgaac cacctgttac tataacatct cccgttgtag
    41581 cacttaggtc aaaaatccaa tctcccactc ctgtcatata aggtaaaatc gttcctccag
    41641 ttacaagtat acgatatcct ctatttgttc ctggattaac acatccatcc ccaaatgcac
    41701 taccatgtgc acctccatat gaataggaaa gtccaccatt aattgtaata ttcccactat
    41761 aaccagaacc tgtgctagga tctactactg agggacttaa tgcattcttt gttgttgtat
    41821 agtatcctcc acctattcca gctccatggt atgcaccttt agcagttata gtaccaccat
    41881 tgattgtgat atctccacca ctaccattac cgcctccacc tattccagct ccatatcctg
    41941 tattacgact cgattcttga cctatagcag ttatagcacc accattaata ataatatttc
    42001 ctccactttc agtatttcca ccgccaatac ctgcagaata ataatttcca acaactttta
    42061 aacttcctct attattacta tctagtagtg taaggcgatc tccttttttt aacttgtctc
    42121 cattttctag agttacatct ctaggaattt ttccaccctc agaaactata aactcgcctt
    42181 caatatctct atttacaaca gaatcatcaa taattagagt agtacctttt cctgctctca
    42241 tacctggata atatacatca cctgactcta gcttgttttc actaccatct tgtaatataa
    42301 catgtgcata tgcaccacca tctttatctg gtgttaaagt aaatgggcta ttagatgcag
    42361 ttttaatatt tactccatca aatattaatt cagcttttac atctttaggt ataacaatcc
    42421 tatgtgttgt tggagtattt ggattattat tttgaattgt catttgtttt ccactaagaa
    42481 tagttaaaac gccacttgca aatttaaaat cgacatttat ttctccacca ctaattttaa
    42541 agtctcctac agatgtagga tcttctgcag tggcttcttc cacaactaat gcattcatat
    42601 ctccttctgc atttcctgca ttagaatctt cttctgcatt tcctgcatta gaatcttctt
    42661 ctgcatttcc tgcattagca tctccttctg catttcctgc attagcatct tcttctgcat
    42721 ttcctgcatt agcatcttct tctgcatttc ctgcattagc atctcctcct gcatttcctg
    42781 cattagcatc tccttctgga tttcctacat tagtatctcc tcctgcattt cctgcattag
    42841 catccccttc tgcatttcct gcattagcat cccctcctgc atttcctgca ttagtatctc
    42901 cttctgcatt tcctgcatta gtactttctt ctatagtatc tgcgaaagta tttgacgcat
    42961 taaatgatgc tactgatcca agtcccattg agatacttaa aatcattgat agtgctttct
    43021 ttttacattt ttgttgcata aattccccct ccccttaata atttttaata tacactgtat
    43081 acatccttat ata
//
LOCUS       DAELNY010000017        42394 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.017, whole genome shotgun sequence.
ACCESSION   DAELNY010000017 DAELNY010000000
VERSION     DAELNY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.017"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..887
                     /locus_tag="KPE55_000762"
     CDS             <1..887
                     /locus_tag="KPE55_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0491859.1"
                     /translation="GNYGILNQIRINKCVSIDKCKEILNEEKEIIYAYIIKIAFEEKI
                     DLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDMEKIRVYKAISKVV
                     LESISLSDEKYKEIFYFYIMYSYKYIRYIYSNLSDFELKKFVCNDLDKFTIEFKHLLD
                     IKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKESEEFKDLKYSFII
                     SIENMIETGKINDAKLLVDDYSKTFVDDVRILNIKGILYMFENKLDKADFMLKKALSL
                     DVENEDTIYNIEYLKSLR"
     gene            911..2788
                     /locus_tag="KPE55_000763"
     CDS             911..2788
                     /locus_tag="KPE55_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0491860.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            2781..4895
                     /locus_tag="KPE55_000764"
     CDS             2781..4895
                     /locus_tag="KPE55_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0491861.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            4904..5899
                     /locus_tag="KPE55_000765"
     CDS             4904..5899
                     /locus_tag="KPE55_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG0491862.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            5991..7223
                     /locus_tag="KPE55_000766"
     CDS             5991..7223
                     /locus_tag="KPE55_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG0491863.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            7243..8187
                     /locus_tag="KPE55_000767"
     CDS             7243..8187
                     /locus_tag="KPE55_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG0491864.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            8281..8688
                     /locus_tag="KPE55_000768"
     CDS             8281..8688
                     /locus_tag="KPE55_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG0491865.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            8775..9866
                     /locus_tag="KPE55_000769"
     CDS             8775..9866
                     /locus_tag="KPE55_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG0491866.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      10175..10265
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            10677..10994
                     /gene="flgB"
                     /locus_tag="KPE55_000770"
     CDS             10677..10994
                     /gene="flgB"
                     /locus_tag="KPE55_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG0491867.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            11002..11406
                     /gene="flgC"
                     /locus_tag="KPE55_000771"
     CDS             11002..11406
                     /gene="flgC"
                     /locus_tag="KPE55_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG0491868.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            11416..11733
                     /gene="fliE"
                     /locus_tag="KPE55_000772"
     CDS             11416..11733
                     /gene="fliE"
                     /locus_tag="KPE55_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG0491869.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            11758..13308
                     /gene="fliF"
                     /locus_tag="KPE55_000773"
     CDS             11758..13308
                     /gene="fliF"
                     /locus_tag="KPE55_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG0491870.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            13321..14391
                     /gene="fliG"
                     /locus_tag="KPE55_000774"
     CDS             13321..14391
                     /gene="fliG"
                     /locus_tag="KPE55_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG0491871.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            14384..15115
                     /locus_tag="KPE55_000775"
     CDS             14384..15115
                     /locus_tag="KPE55_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG0491872.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            15119..16438
                     /gene="fliI"
                     /locus_tag="KPE55_000776"
     CDS             15119..16438
                     /gene="fliI"
                     /locus_tag="KPE55_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG0491873.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            16469..16915
                     /gene="fliJ"
                     /locus_tag="KPE55_000777"
     CDS             16469..16915
                     /gene="fliJ"
                     /locus_tag="KPE55_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG0491874.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            16944..18149
                     /locus_tag="KPE55_000778"
     CDS             16944..18149
                     /locus_tag="KPE55_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG0491875.1"
                     /translation="MKIQADSYNIIKDIGDRKSSPKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            18167..18775
                     /locus_tag="KPE55_000779"
     CDS             18167..18775
                     /locus_tag="KPE55_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG0491876.1"
                     /translation="MYGIATALAGTKGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            18802..19785
                     /locus_tag="KPE55_000780"
     CDS             18802..19785
                     /locus_tag="KPE55_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG0491877.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            19798..20001
                     /locus_tag="KPE55_000781"
     CDS             19798..20001
                     /locus_tag="KPE55_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG0491878.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            20001..20819
                     /locus_tag="KPE55_000782"
     CDS             20001..20819
                     /locus_tag="KPE55_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG0491879.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            20809..21504
                     /locus_tag="KPE55_000783"
     CDS             20809..21504
                     /locus_tag="KPE55_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0491880.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            21516..21959
                     /locus_tag="KPE55_000784"
     CDS             21516..21959
                     /locus_tag="KPE55_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG0491881.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            21974..22348
                     /locus_tag="KPE55_000785"
     CDS             21974..22348
                     /locus_tag="KPE55_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG0491882.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            22349..23014
                     /gene="fliP"
                     /locus_tag="KPE55_000786"
     CDS             22349..23014
                     /gene="fliP"
                     /locus_tag="KPE55_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG0491883.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            23027..23296
                     /gene="fliQ"
                     /locus_tag="KPE55_000787"
     CDS             23027..23296
                     /gene="fliQ"
                     /locus_tag="KPE55_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG0491884.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            23308..25122
                     /locus_tag="KPE55_000788"
     CDS             23308..25122
                     /locus_tag="KPE55_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG0491885.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDIAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            25131..27206
                     /gene="flhA"
                     /locus_tag="KPE55_000789"
     CDS             25131..27206
                     /gene="flhA"
                     /locus_tag="KPE55_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG0491886.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMIIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTVGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELISNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            27181..28197
                     /gene="flhF"
                     /locus_tag="KPE55_000790"
     CDS             27181..28197
                     /gene="flhF"
                     /locus_tag="KPE55_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG0491887.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENIEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            28194..29072
                     /locus_tag="KPE55_000791"
     CDS             28194..29072
                     /locus_tag="KPE55_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG0491888.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            29086..29787
                     /locus_tag="KPE55_000792"
     CDS             29086..29787
                     /locus_tag="KPE55_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG0491889.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            29803..30225
                     /locus_tag="KPE55_000793"
     CDS             29803..30225
                     /locus_tag="KPE55_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0491890.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            30210..30395
                     /locus_tag="KPE55_000794"
     CDS             30210..30395
                     /locus_tag="KPE55_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0491891.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            30436..31209
                     /gene="flgG"
                     /locus_tag="KPE55_000795"
     CDS             30436..31209
                     /gene="flgG"
                     /locus_tag="KPE55_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0491892.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            31230..31991
                     /gene="flgG"
                     /locus_tag="KPE55_000796"
     CDS             31230..31991
                     /gene="flgG"
                     /locus_tag="KPE55_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0491893.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            32014..32895
                     /locus_tag="KPE55_000797"
     CDS             32014..32895
                     /locus_tag="KPE55_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0491894.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            32898..33272
                     /locus_tag="KPE55_000798"
     CDS             32898..33272
                     /locus_tag="KPE55_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0491895.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            33304..34707
                     /locus_tag="KPE55_000799"
     CDS             33304..34707
                     /locus_tag="KPE55_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0491896.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            34823..36760
                     /gene="htpG"
                     /locus_tag="KPE55_000800"
     CDS             34823..36760
                     /gene="htpG"
                     /locus_tag="KPE55_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG0491897.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            37148..38278
                     /locus_tag="KPE55_000801"
     CDS             37148..38278
                     /locus_tag="KPE55_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0491898.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            38505..39527
                     /gene="splB"
                     /locus_tag="KPE55_000802"
     CDS             38505..39527
                     /gene="splB"
                     /locus_tag="KPE55_000802"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG0491899.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            39709..40620
                     /locus_tag="KPE55_000803"
     CDS             39709..40620
                     /locus_tag="KPE55_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0491900.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            40635..41051
                     /locus_tag="KPE55_000804"
     CDS             40635..41051
                     /locus_tag="KPE55_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0491901.1"
                     /translation="MNWQDAFFQDTKPKFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(41151..41543)
                     /locus_tag="KPE55_000805"
     CDS             complement(41151..41543)
                     /locus_tag="KPE55_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491902.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            41684..42079
                     /locus_tag="KPE55_000806"
     CDS             41684..42079
                     /locus_tag="KPE55_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0491903.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        17350 a         4280 c         7664 g        13100 t
ORIGIN      
        1 aaggtaatta tggcatttta aatcaaataa ggataaataa atgtgtatct atagataaat
       61 gtaaagaaat actaaatgaa gaaaaggaaa taatttatgc atatattata aaaatagctt
      121 ttgaagagaa gattgattta ctagatattt tttatgaaat ggaatatgta tggatagaga
      181 aatatctaaa atatctttta gcattagaaa gaagttttaa tttagtacta tataagtaca
      241 taattagtcg tccaaataca tcagatatgg aaaagataag agtatataaa gctatatcta
      301 aagtagtttt agaaagtata agcttaagtg atgaaaaata taaggagatt ttttattttt
      361 atataatgta ttcttataag tatataagat acatatatag caatttaagt gattttgaat
      421 taaaaaaatt tgtttgcaat gatttagata agttcactat agaatttaag catctacttg
      481 acattaaaaa taaagtaaat aatgctaatg aaaaattaga gtatatacat agtatgagga
      541 aattactaaa tgagtatcct ttttataata aaataataaa aatacttata ggtgaattag
      601 aagaaaatat aaaagaaagt gaagaattta aggatttaaa atatagtttt attattagta
      661 tagaaaatat gattgaaact ggcaaaataa atgatgcgaa attattggta gatgactatt
      721 caaaaacatt tgtggatgat gtaaggattt taaatataaa gggaatttta tatatgtttg
      781 aaaataagct tgataaagct gattttatgt taaaaaaggc attatcatta gatgtagaaa
      841 atgaagatac tatatataat atagaatact taaaaagttt aagataagtc aaaaatagga
      901 gaattgaaga atgaatatac caatagtggt taatgaaaaa tctaaagaca taataaaaga
      961 ttggcaatat acatgtacaa atgaagatat aaaatttaca agcataataa tattgacata
     1021 taatcaaata gaatatacta aattatgtat tgaaagtatt aggaaattta ccccaaagaa
     1081 taaatatgaa ataataataa ttgataataa ctcttctgat ggaacagttg agtggttaaa
     1141 gggacaatct gatataaaat ctgtgtataa taacaaaaac ttaggatttc caaaaggatg
     1201 caatcaaggt attgagttag ctactggtga aaacatattg ttgttaaata atgatgtaat
     1261 agttactcca aattggctct ataatttgaa taaagcttta tggtctcaag ataatatagg
     1321 tgctgtagga cctattacga atgcatgttc atattatcag caaattgatg ttgattataa
     1381 gtcaattgag gacatgttta tatttgcaca caaaataaat aaatctaata ttaacctatg
     1441 gaaatataga ttgaagttag ttggattttg catgttaata aaaaaaactg ttctggataa
     1501 tgtaggggtt ttagatgaat tattcacacc tggaaatttt gaagatgatg atttatcttt
     1561 taggatgata gagtcaggat ataagttatt attatgccag gatactttta tacaccattt
     1621 tggaagtgtg tcttttaaag aaaatcgtaa taaatataca gatataatgt atattaatag
     1681 aagaaaattt gaaactaagt ggggatttaa tccagaatat agtggaatga taagaacaga
     1741 tttgatttct aaaatagatg atagtttgga ggcaaatatt aatgttttag aaataggatg
     1801 cggtataggg gctactcttt tagaaataaa aaatatatat aaaaatgcat caatatatgg
     1861 aatagaaatt agtgagtcag caggtaaact tgcaaagtat agtgcagatg tacttatagc
     1921 aaatatagaa aaagtaaaat tggattataa aaaagatttt tttgattata taatattggg
     1981 agatgtactt gaacatttga ttgacccatg gaagatagtc aatgaattga aacaatatct
     2041 aaagaaggat ggttatctgg tatctagtat accaaatatt atgcatgttt ctgttttaag
     2101 aaatcttgtg ttaggtaatt ttacatatga agattcagga attttggaca agactcattt
     2161 aaggtttttt acattaaatg agataacaaa aatgtttaat gatagtaatt acataattaa
     2221 tagtattaca gctactagta tgccactttc ggaaattgat aatagcttca ttgatacaat
     2281 atgtaaggaa agctgtaaag aaatgagaca acagtacgaa tcttatcaat atatagtaaa
     2341 agcctctaaa atcctagata ctaaaagata ttctagtatt agtatgatta atttaaaata
     2401 cgctttaatg agaattgata atgaattaga tgtagatagt aatttaagtt atatatttga
     2461 taattataat ataactgaac catgttttgt tgatgatatt gaatatctaa ttgatacatt
     2521 tgtgataaat aaagcaactc tattaaatag attaggagtt gaggcattta acagaggtct
     2581 aggagatttt agtataaaca tgtttatgac agtattagag atggatagag atgatattga
     2641 ctcaatatat aatataacaa gtgtattatg tgaattagaa gaatttgata ctgcttataa
     2701 tttaattaaa aatagtagcg aaaaagtaca gagtgaaagt gatatacaag agatactttg
     2761 ttttattgag gggaagaaat atgaataatg ataagatatg ttttataacc tgtgtaaatg
     2821 atgaaaggct ttatgaagaa tgtcttgtat atataaatag cctagacatt cctaaaaact
     2881 ttagtataga aacagtagct ttaagaggag ccgattctat tgcctctgct tataataaag
     2941 ctatgagaga aacagattct aagtataagg tatacttaca tcaagatact tttataatta
     3001 ataaaaattt catacatgat attttagaga tatttaaaaa aaatacagat gtcggaatga
     3061 ttggtgtagc tggagtaaaa gatatgcctg tgaatggaat ttggtgggat tctgataaaa
     3121 aagcagggaa agtatatgat agtcatacag gtaaaatgga tatttatgaa tttagtaact
     3181 ttgaagaatg ttttaatgaa gttatggcta tagatggatt aattatgatt actcaatatg
     3241 atgtattttg gagagaggat aagtttgatg ggtggcattt ttatgatttg tcacagagta
     3301 tggagtttag acaaaataaa tacaaagtta ttgtaccaaa acaaaataag ccttggtgta
     3361 tgcatgattc tggagtagta aatataaata atggatatga tgaatatagg ataaaatttg
     3421 taaaagagta cataaatctt gatttatata gagaaaattt ttatgaattt gataagaaga
     3481 aagcaagtac catacttaag agtaataagc atgtaatgga ttgtatgaaa gaggagctta
     3541 cagaacaatt aagattaaag aattacaagg atgttttagg tattgtttct aaaattgctc
     3601 atttttctgt ttttaatcat actggtttgt attcttgtga agaatgtgag agagcattgc
     3661 tagaatgtgc aaataattta ccagaaatag aaaatacgaa attatttaca aaaaaagatt
     3721 taaaaagaag aaaagtatta catgttttaa gtgaaggata ttcaacagga ggtcatacaa
     3781 gactagttaa aaattggatt gaaattgaca aagactctgt tcattcagtt gtaactacat
     3841 ggcaatcaga tacactgcca gactggttat taaaagcaat aaatgatagt ggtggcgaag
     3901 ttatatcttt ggatttaatt tcagatgatt atttaaaaag agctgtagct ttgaggaaaa
     3961 tagcttatga ctgggctgat atagttattt tatatgttca tatgtgggat acaatacctg
     4021 ttgttgcttt tggagttgat gggaaaatac ctattttaca tgttaatcac tatgatagta
     4081 ctttttggtt agggacaagt attgcagata ttgtcattga ttttagcgaa actagtcaat
     4141 taattacatt agataaaaga ggcatagata aatctgaaat attaccaatg ccattaaaag
     4201 aaaaagaagt attttcaaaa tctgaagtta gaaaacgata taatttagag gatgatgtga
     4261 caataatatt gactattgct agtgaaagaa aatacaatag tgtaaatgat attaattact
     4321 ttaaaattgc taaaacattg attgaaaata cagataatac aattattatt gttgtaggag
     4381 ttgatacaga gaatctctat tggaaaactt taagtaatgt tacagataat agagttatat
     4441 gttttggaac aacagcaaat atagatgatt tttataagat ttcggatata tacctagagt
     4501 ctttcatggt aggaagtcat acttcaaaat tagatgcaat tttatatgat ttaattcctg
     4561 ttaagtttaa aaatgaagtt tcaccaattt ttacaaatat gtggaatttt atagataagt
     4621 ttgattataa gaatataaaa gaggttatag acatgataag aaggtacaat actagtgaat
     4681 ttgacaggga taagataaaa gcaaaacaat cggaaataaa acattttgtt atagataaac
     4741 actgtattga aataaaaaac tatatagaca aaatatataa tgaagttaat catcataaag
     4801 ttaagtatga tttatataaa aatgatgaaa tcgaggacta tcagcttttt gtagcattat
     4861 atggctatat ggtaaaaaat aaaaacatgt cataggtggt aaaatgaaca atattcatat
     4921 caataaattt gaaaaaagct gtgaaaaaaa ctgggattat tttgtagaaa ataaatctat
     4981 aaatggtaca tttctgcaaa ctagaaattt ccttaattat catgaggagg gaaaattcac
     5041 tgaccactca ttaataatat gtaaaggtga agaaatacta gcagtcgtac ctgcatgtga
     5101 aacttatgaa gatggtgaaa gaatattttt ttctcattta gggagtactt ttggtggcat
     5161 tataataaga agtggtttta atgatattag gcatgtaaat gccattacaa ataagttaga
     5221 aatatattta caggaacaaa atttcgataa agtatattta aaaaatacat caagaatatt
     5281 ttgtaaagag gatacttcat tactagaata ttttttattt aagaataatt acatgtttta
     5341 tgatgaatta agtatgtttt tagaattaag taagtataaa gaagatatat tagccaattt
     5401 taaatacagt aaaagaagag attttaaata ttctttgaag aataatctta cctttaaaag
     5461 actgtatagt gatgaagata tagtttgttt ttttaattta ctttctaatt gtttagatag
     5521 taaaggagct aaagtaatac attcagtaga agaacttttg gagtttaaaa ataatagatt
     5581 aaaagatatt gtagaatttt atgcagtata tattgatgac attatgattt gtgggagcat
     5641 ggtatttaac tttcaagaaa gagtttatca tactcaatat ctagcatcaa attcgacata
     5701 taataaatat tatccaatga tatttttata ctataatcta ataaaagatg ctttagagag
     5761 aaattttaag tatctttctt ttggaattag cactctaaat agaggcaaat acttagatga
     5821 gggtttagct acatttaaag aaggttttgg ttcaagtgca atttgtaaca gaacatacta
     5881 taaaaatata aataagtgag aatcatgttt tatagagttt ttagagtata gtaaatatct
     5941 agtactataa tattacataa aaataattag tttataaaga aggtttgtat atgaaaagat
     6001 taatgatttt aggctcaatt aagtattttg agaatattgt tatgtcagct aaaagagagg
     6061 gaatatacac aattgtttgt gataatagaa ttgatactcc tgcgaaaaag atttgtgatg
     6121 aagcaataga tgttgatgta tttgattttg ataaaataaa gaatatagct atagataaaa
     6181 agatagatgg aattttaact ggatttacag attctttaat gaagccatat gtttatattg
     6241 caaatgaact aaatttgcca tgtgtgattt cttgcaatca acttgaatct gtaactaata
     6301 aggctgttat gaaaaattat tttaaaaata atggaatacc tacaactgat tattgtatta
     6361 ttgaaagttt gaaagattta gaaaaaatac aaggattaaa ttttccactt gtaataaaac
     6421 ctgttgatgg ttatggttca agaggtgtat attttgtatc aaacatagaa gaattaatag
     6481 aaaaatttga atattctaag gaagcctcgc aagatggaca agtaataatt gaggaatttt
     6541 atgaaagtga ggaaatacaa ggactagctt gggttcataa tggagagtca catgtatttt
     6601 atattggaga tagagagctt gtaaatatac atacaggaag agctggaaaa ccagatagat
     6661 taatttatcc atcaaagtat tgttttcaat atgaagaaga aataaaatcc atatatcaaa
     6721 atataacgca ggcttttggc ataaaaaatg gacctcttta tgtacaaatg ttagtgggag
     6781 catatggaat aaaggtatct gaagtaatgc caagattacc tggaggttgt gactatatgg
     6841 ctatttctca aataacagat ttagacatag gaagtttatt tgcaaacttt agtgtgaata
     6901 atgaagtaaa ttttgatgaa ataaagaaat ataatatgca tctatcaaaa tgtgtatatg
     6961 cattaccaat atacataaga cctggcacaa taaaaaagat aaataatata gataaaatag
     7021 aaaacttaga ttatgtcact caatttctat tactagtaaa agaaggagat acaataggat
     7081 tatatgaaga tgagagacag gattgtggaa gagtttatgg agtagcaaat aatgtatttg
     7141 atgcaaatat aaagaagaaa tgtattcatg aaatgataga aatacttgat gaaaataatg
     7201 aaaatatgat agaaaatttt taaataaggg agtatgatag atatgaagct aataactcat
     7261 gaacaaatta tgtcattaaa catttctcag aaacaaggat acgagtggat aaaagaatcc
     7321 tttttaacaa aaaaagattg tcaattacca ccaaaaataa gcatggtaga agaagggcat
     7381 gtattttaca atgtaatgcc gtccctaaat cataatcata agattggtgg aataaaaatg
     7441 gttacaagat tgccagggag aaatccaagt ttaaattcag agatatttct atataatatg
     7501 gaagatgcta aattaaaggc tattttagat ggcaattata ttacagcttt taggactgct
     7561 gctgttgctg ccttggctat cgatacattt gctaaaaaag attttaatac tattgctatt
     7621 atgggtttag gtgtaactgc ttctgctata atgaatatgc tttcatatac tcttaaagat
     7681 aaaaatataa atataaagtt acttaaatat aaagaccaag cagagtcgtt tgtaaaaaag
     7741 tatgaaaaaa atgaacaatt tagttttgaa atatgtgaca cacatgaaga acttattgta
     7801 gattcagatg tagttatttc tactgtgaca tatacagatg aatatttatc agaatttaaa
     7861 tggtttaaaa aaggtgcttt attattacct gtacatgtta tgggatttgg aaatgttgat
     7921 tatctatttg ataaagtata tttggatgat tataatcatt tgaaacatct agaaaatata
     7981 gatagatata aatattcttg tgaattttat gatgttttaa gtaaacaagc taaaggaaga
     8041 gaaaatgatg atgagagaat aatatcttac aatataggtt tgtcaataca tgatatatta
     8101 tttgcaaata aaatttacga attgtgtgaa acactaagct gtgaagaaat agaattaaaa
     8161 gaaccaaaag aaaaatattg gatatagttt atttcattca tatttacaat agtttattgt
     8221 aaatatgaat atgaagtaat atattgttag aaaattagca tgaataggat agataaattt
     8281 atggataaat ataatttaat taggttcgta tctattggaa acgaatatat aggaaattta
     8341 gttgcattag aatcaaataa agcaatacct tttgatataa agagagttta ttacatatat
     8401 gatgtaccaa acaatgtaaa gagaggattt catgcacaca aaaacttgga acaagtacta
     8461 gtttgcctaa atggaagtgt gaagataaaa tgttatgatg gtgtaaaaga gactatattt
     8521 aatttacata aaaatgatgt tggattatat ataggaacgg gagtgtggca tgagatgttt
     8581 gattttgatg aaaaaactgt tttaatttct atagcatctg aacattataa tgaagaagat
     8641 tatattagag actataaaga atttttaaag tatttaaata atgtgtagtt attatacaat
     8701 tagtgatgta ttttcttaat taaataaatt taagatagcc acatagagaa tagatagatt
     8761 aggatgtgaa tttattgttg gttaaattta ataatttatt aaaacagttc caaactcata
     8821 aagaagaata tgaacagaag attaaggatg taatggaaag tggatattat atccttggaa
     8881 atgaagtaaa gcattttgaa gaagaatttg ctaactatgt aggtagcaaa tattgtgttg
     8941 gtttagccag tggtttagat gcactgataa ttgcttttag agcattagga ataagcaaag
     9001 gtgatgaagt aatcgtgcaa tcaaatacat atattgctag tgttatggga atcagtataa
     9061 atggagcaac accagtattt gtagaaccaa atgaatatta taatatagat tcatctaaga
     9121 tagaagaaaa gataaatagc aaaacaaaag ctatattagt agtgcattta tatggtcaag
     9181 caagtaatat gaaagatata caagaattat gtgataagta taacttgtat ttaattgaag
     9241 attgtgctca atctcatgga gctaaatatg atggaaaaat gacaggaaca tttggtgata
     9301 taggttgttt tagtttttat ccaagtaaaa atttgggtgc atttggagat gcaggagcta
     9361 tagtaactag taatgaggaa atagctgata aagtgaaaat tcttagaaac tatggctcag
     9421 aaaagcgata ttataataaa tttgtcggat ataattcaag attggacgaa attcaggcag
     9481 gtttattaag agtaaaactt aaatatctaa atgaaattga aattgaaaaa tcaaaaatcg
     9541 tagatagata tcttaatgaa attaataatc ccaaagtcat acttccaaag atagaaaata
     9601 attgtactca tgtatggcat ttatttgttt tacaagttga aaaacgagat acatttatgg
     9661 aatacttaaa gaaaagtggt atagagtcta taatacatta tccgatacca ccacatctat
     9721 ctgaagcata tgcttatttg ggatataaaa aggggagttt taatatagct gagaactatt
     9781 cagataaagt tgttagtata cctttattta ttggtatgga agatgaagaa gttagttata
     9841 taattaaaat tataaatgaa tattaaacta ctcataataa tatcaaaaat aaatttttta
     9901 tatatcattt atttaaattt cgttaattga catttttaaa tattgatata ttgtacaaat
     9961 aaaattgaaa tatatggaaa aatatagaat taagatgaga tttgtcttat atttttataa
    10021 attaaaaata ttttcaaaga gtttattata aaattgtaaa ctatagtgtt tataatcttt
    10081 gtaattattg gtaattaaat ttaaaaaata aaatatttaa ttatacacaa aaaagatatt
    10141 taaaaaattt gttatttatt gtatacttaa gttaaaacta aataggcaaa tctagagaaa
    10201 tctagtgacg caaagctata gggactaagg tttatataca taaactatgt cagccagttg
    10261 ccaaaaagag tcctaggtgt attgtatacc taagaaaagt ctataatgac tgcctttttg
    10321 gcagtcattt tgttatggaa aaatcacttt atttcagtag aaaatacaca taataacata
    10381 ttttaccaat tataacataa aataaagaaa aattatctca aaaagttact attttacaaa
    10441 aaaaaatgac ttctttattt atttaaatta atcgcatttc cattacatta atacaataat
    10501 gtatcaattt agtaagaaaa ctaatatttt tttaatgaag tcccatgaaa atataaaaaa
    10561 attttaatat gtatttaaat atttcttcga aaagttgctc ttttttgtaa tatagcaact
    10621 tcattttttt tattaataaa caaaatagat aaaatatatt ttgaggaggg gtgagtgtga
    10681 atacatatga cattataaaa atgggattag atgcaactaa tctaagaagt cagacaatag
    10741 caaataatat agcaaatata aatactgaag gatataaaag acattatata acttttgaag
    10801 aaacactaaa tgatgctatg cctaaaatag aagaaaaaac agacaattct aaaggtttaa
    10861 gagtagatgg taataatgta gacatagaag aagaaaaagt aaatcaagca atggcaacat
    10921 tacaatataa tgctttgata agttttgcaa atggaaaaat agcaatgacc aaatcaataa
    10981 taagtgggag gtaattagaa gatgagtgtg ttcagtggaa tgagaatatc tgctagtgga
    11041 ttaacagcag aaagtttgaa aatggatatc atatcatcca atgtagctaa tataaatgct
    11101 acaagaggtc caaatggaga agcatatagg agaaaagttg tttcatttga agaaaactat
    11161 gataataaat taggtatgct aggagtaaaa gttacatctg ttgaagatga taaatcacct
    11221 cttaaagaag tatatgaccc atctcatcca gatgcaaagc caaatggata tgtagaatat
    11281 ccaaatgtaa atatattggt agaaatgaca gatttaataa cagcttcacg agcatatgat
    11341 tctaacgtag atacattgaa tgctcagaag tctatgatat caaaagcttt ggaaatagga
    11401 aagtagggat aaattatgcc aattacacaa attaacaatt taatgcaagg tgcaaagtta
    11461 gataatttga ttggagaagc aaagccatta actcttaata ataaagaaga aaaaaagaat
    11521 tttgaagatg taataataga tactatagat aaagttaatt ttcaacaagt aacagcagat
    11581 gatatgaaag aaaaatttat aaaaggcgaa gatgtttcta tgcatgaagt tatgttaata
    11641 ggtcaagaag cacaactatc tatgcaatat ttaatagaag ttagaaataa gattcatgat
    11701 gcatatcaag aaatgaataa ggtgcaaatt tagccttttg aacaggagta ataatcaatg
    11761 ataaccagtt ttaaaaataa gattaaggag caagttgcta aatctaggac aaagataagt
    11821 tctttaaaaa agggaactaa aatagcatta ggtatagggg ttattgctat atgtttagcg
    11881 ctagtattta cagcaatatc tgcgagcaaa aataagtatg cagtcttatt ttcagaacta
    11941 gatgctcaag atgctgcaga tatatcagca gagctagagt ctaaaaagat agatactaag
    12001 gtaaaaggaa gtacgattta tgtacctaga acagaagttg ataagttaag gctggagtta
    12061 gcgccaagtc ttacaggtgg ttctaaagga tttgcactaa tggatgatag ttcatcaata
    12121 gggcttactg atgaagaatt tcagattaag aaacaaagaa tgatacaagg tgaaatagaa
    12181 aaaacaataa agagttttcc acaagtggaa gacactagag tacatataac atttggagag
    12241 tcatcagtat ttgagaagga ggctgttcca gggagtgcag cagtttattt gatgctaaaa
    12301 ccagggacga ctttagaaaa aaaccaaata aagtctgtta tggcacttat atcaggaagc
    12361 gttacaaata taccagagaa aaatgttcag gttattgata acaaaatgaa tttattatct
    12421 gaaggtattt tggaggatga aaatgttgat gaaaataatc aaccaataaa tagtggcatt
    12481 agtgttgata aaaataaggc atctgaacaa gcattgaata aacaactaga aaagtctatt
    12541 ttagaaatgc tagaaccgat atttggtaag ggaaaggtga aagctacagt aaatgctgat
    12601 ttaagttttg atacagctga aaaaactgag ataaaaattg accctgataa agttgcaata
    12661 aaggagacaa ggtcgaaaaa ttcatctaaa tctgctgcaa ataatgttca aggtgtagat
    12721 gctaacatga ataaccaagg aaataacaat ggtactaata cagaagatag tttagatgag
    12781 agttttgagt atgaaacagg taagattgaa actcacacta tagttgcgcc tggagaactt
    12841 aatagattga cagcttctgt tgcaatagat ggaaaaatag ctaaaggtgt tcaagctgat
    12901 gtagaggata ttgttaataa tgctataggt atggatggag cgaggggaga tactcttagc
    12961 gttgtttcaa tggtatttga cccagaaggt aagaaagagg ctaaacaaga gctggaagag
    13021 attaaaaaag aaggatttaa aaagaacata ataaaagcag ttattggagt agtagcagta
    13081 atagcagttg gagctattgg atacttaata tataagaaaa gaagtgaacg agaagatatg
    13141 gattatgaag atgaagatta tatttctaaa tctgattctg ttgataggaa ggataatcct
    13201 ctattaggag atgatccaga actgacatta gaggaagctg ttaaaatgta tgcagaagaa
    13261 aagccagacc aagtaacaga gataataaag acttggttaa atgagtaagg gggatagaaa
    13321 gtgtccacgg aaattacaaa tgttaatgcg tttgacttaa gtgatataaa tgaaagtgat
    13381 aagaatgaga aggtagaagg tgagggttta aaaaaagctg ctgtattatt aatgacatta
    13441 ggcccaaatg tgtctagtga tataataaaa agcttgcctg ataaacaagt tcaaaaaata
    13501 ggtttggaaa ttgcaaatat ttcttcaata gcttcaaaag aaagaagaga aatcttaaaa
    13561 gagtttgtag aaataaatag ggcaaaagat tatgttatag aaggtgggct tgaatatgca
    13621 agagagttat taagtaatgc tttaggaaat gttaaagcaa acaaattact tgaaggtata
    13681 tcaataaata caagtacaaa gccttttaaa catattataa aactagatgt aaagaagata
    13741 ttagaaatac ttaggaatga aagtgcacaa actatagccg ttttacttgc acatatggaa
    13801 ccagaaaatg gagcagaagt tttatcatac ttagattttg atttacaaaa tgaagtagct
    13861 atgagaataa gctcaatggg ttcaatatct ccagaagtaa taaaggtaat tgaaagttca
    13921 ttagaagata aattacattc aataagtggt gatgatttta atgattatgg aggggtgtct
    13981 accttagttc aaatattgat taattctgat agaaaaacag aaaagaatat tttgggaact
    14041 attgaagaac aaaacccgga tttagcagct caaattaaat ctcatatatt tgtctttgaa
    14101 gatattgtca agttagatga tataagcata caaaaagtgt taaaagaagt gactatgaaa
    14161 gatttagtat atgccttaaa aggtgtagat gatgagattt cagatgctat atatagaaat
    14221 caatcttcta gagcagcaga tgcacttaaa gaagaaatgg aaatggttgg tgttataaag
    14281 gttacacaag ttgaggcttc tcaacaaaag atagctaata tcgtaagaaa acttgaaaga
    14341 gaaggtgcca taactattgt aaggaatttt ggtggtgact ttgttgaata ataagattat
    14401 taaaagtaaa aaagcattat ataaaggagt gctagattta accataatag ataaagttag
    14461 agtgggagaa tcaaatgtaa ataaagaaaa tgattgtaga tttagggatg aggtaataaa
    14521 agaagctata gaatctgctg agataaaaaa gaatgagata ttagaagaag caaaaataaa
    14581 tgcacaaaat atagaaaagc aagcatatga aaaagggtat agtcaaggtc aaaaaaatgg
    14641 atatgaggat ggatataaag agtcatatga tgaatatacc caaaaagcta aagatgaagc
    14701 agattttata aaaaatcagg caaatttaat cttattacaa gctaatgaga agatgactga
    14761 gtacataaaa gaaaaaaata aagagattgt aagacttgct attaatatgg ccagttctgt
    14821 tttaaaaaag catttcgaag atgaagaatc tatgagtgat ctgttaaagc aaattattca
    14881 agattatgaa ggaagtagtt catttatagt aagatgtaat tctttctata aagataatat
    14941 agagaaagag tttgaattat tcaaagagga aacatcacta aaatcaagtg tatttgttat
    15001 tgcagatgat agtatagata aaggcaatgc tattatccaa aaggaaaatg gaagaataat
    15061 agttggtatt gattgtgcta tggaaaaact aaaagaagaa ttgcttggtg aataaagtat
    15121 gtataaaact gatatatcta tagattttga caaaataaat aagaaaatag aaaatttaga
    15181 actcactata tcagaaggta gagtaaagaa gataatagga cttactgtag aagttgaagg
    15241 aataaaagct tttgttgggg agttatgcgt tatatacaac caagttaata aaccagtgaa
    15301 ttgtgaagta gtaggtttta aagataaaga agtcatatta atggcgttgg gagaattaac
    15361 attgatagca cctggttgta aagttatttc taaggggata cctttaagtg ttatgtgtag
    15421 tgataattta ctaggtaaag tacttgatgg tcttggaaat cctatagaca actcagatgc
    15481 tgttttagga gatagataca acctgaacaa tgaacctcct gatccaatga agagaaaaaa
    15541 gatacgaaat ataatggaaa caggtgtaag agcaatagat gcatttacta cttgtggtga
    15601 aggacagaga atcggcatat ttgcaggaag tggagtaggt aagagtacga ctctaggtat
    15661 gattgctagg aatgcaaaag cagatgtaaa tgtaattgct cttattggag aaagaggaag
    15721 agaagtacta gattttattg ataaagattt aggtgaagag ggaatgaaaa aatctgttgt
    15781 tgtgtgtgca acatcagata aagcccctct agttaggtta aaaggtgctc ttactgctac
    15841 tgcaatagca gaatatttta gagaccaagg caaaaaagta atacttatga tggattcagt
    15901 aactagattt gctatggcac aaagagaagt tggtgtagca ataggagagc caccagctca
    15961 aaaaggatat acaccatctg tatttgcaat gttgccaaaa cttatggaga gaacagggac
    16021 atcagataaa ggttctataa ctgcatttta tacagtatta gttgatggag atgactttaa
    16081 tgagccaata gcagatacaa caagagggat acttgatggt catatagttt tatctagaga
    16141 cttggcaaat aagaatcact atccttctat agatgtatta aacagtttaa gtaggcttat
    16201 gaatgaaatt gcttcaaaag aagacataaa aatagcgtct tttgcaagag acatgcttgc
    16261 tgagtataga gaagcagaag atttgataaa tataggtgca tatgcttcag gtacaaataa
    16321 aaaaatagat gaagcaatct attatcatga gcatattatt aattttttaa aacaagggat
    16381 aaatgaaaag agttctttta atgaaactat aagtagtttg agaagagttt ttgaataaac
    16441 aattaaaaaa atatttgaat aggttgtgat ggaattggat aaaaacttta agtttagatt
    16501 acagaaagtt ttagacttaa aagtgaaaga tgaagaagag ataaaaatgg aatttgctaa
    16561 gattcaacaa aaaaaaatag atatagagac taatttagaa aacttagaaa gtaattatag
    16621 caaatattct ataagtaaaa ataacgatag tattcaaaat caaaaaataa ctattaacta
    16681 tttactagct ttaaacaatt ctataatgga tttatcagaa gagttggata agtcaacaaa
    16741 tgaacttgaa aaagctagaa agcagcttat aagcaaacag atagagagaa aatcattaga
    16801 aaaattaaaa gaaaaaaagt atggacagta ttataaagaa gaaaatttaa aagaacaaaa
    16861 cactaatgat gagtttgcaa gtatgagtta tttaaggaat agacaagttc tataagaata
    16921 gtaggaagtg aggatattat tgtatgaaga tacaagcaga ttcttataat attattaaag
    16981 atattgggga tagaaaaagt tcacctaaga acaaaaatat aaaaaataat aaatttgaag
    17041 ataatctata taaggcaaca gaccataaaa aagttaaaga tagaagggaa gtaagtaagc
    17101 aaaatattag ctcagataaa ccggaaaata gtgataatga gtgtcattta gatgaagata
    17161 aaagtaatca cttagaagat gaaaatacat atgaaaaaga ttgtagtaag aataatgaag
    17221 ctttgcagca attactgaat cttttaaagg ataattataa aaaagataaa gatataaaca
    17281 ctatattgaa taatatagaa aaactaagta tatcagatga attaaaacaa gaacttaagc
    17341 ttaataaaaa tatattaaat aagattgcaa atcaaaattc agagctaaat aaagctaata
    17401 taaataactc taatgatata gaaaatttag acatagttaa attaagagaa cttttggaag
    17461 ataatgctaa taaagatatt gaagtaatta ctaaaactac agaaaatgat accaaaaata
    17521 tagatttgta tagttttgat agcaatagaa tggataatct taactcaaat aaacagaaag
    17581 atgatagctc tgatattttg gaaaaactag caggggtaaa tggaaataag agtgggaatt
    17641 ttggacaagt aataaataaa aatgttgata tgaataaaat taaaaatgat aatgtcaatt
    17701 taaaacagat agaatcaaat gtaatggatg actctataaa agctattaag catatgaaaa
    17761 caaatgatat acaagaattg acaattaaat taagaccaaa agaattagga gatatgaata
    17821 tacaattgtt aaaagatggt gaaagtatga gagctgttgt gactgttttc aataaagatg
    17881 tctttgactc tataaataaa aacatagcag atttaaagca acatcttgaa cttactaatg
    17941 taaatataaa agatgtatca gtgcaaatgc acagcgataa tacaaataca tctgatacct
    18001 ttgataaagc atttgaacaa cagaatagac aaaataacca agaaaacatg agtaagaaga
    18061 atacaaatga taagcataat aatgtagtca tagaagatga cattaaagaa aatactattg
    18121 atgatgatag agtagattta ctagcttagt aagggggaag tattgcatgt acggaatagc
    18181 aacggctttg gcaggaacta agggaaacta taggtcaaat aacttaagcc aacttgaaag
    18241 aaatattaat gatattgcca ataaacaagt tagaagcaaa ggaaatcaga cagtaacaga
    18301 caatgggact ccaattatag gacaaggtga tgatgctgat aaagacttgt ttatgaagat
    18361 acttgtggca cagatgggta atcaagaccc aatgaatccg caagacccaa cagaatatgt
    18421 gtcacaatta gcacagtttg caactttaga aaagatgtca gctatgaatg agaagctaga
    18481 tgtactttta gccttaagta attccactct aataaactct gctttaagta tggcaacatc
    18541 attaattggt aaagaagtag aagtatctgg ggaagagaaa gaagatgaaa acattgtagg
    18601 agtagtaaag tccacatata taaaagatgg ggaagtacac ttagaattag aagtagatgg
    18661 aagtgacaaa actgtaggtg ttaaatatga aaaattactt aaaataggag aggtagatga
    18721 aaattcagga gaagaaaatg attcttcaga acacgaatct ccagaagaag cataatatta
    18781 aatgagaaaa gaggtaaatt catgataaaa gcaatgtatt caggtgtaag tggaatgaaa
    18841 gcaaatcaaa caaaattaga tgtaataggt aacaatgtag ccaacgctgg aactacttct
    18901 tttaagaaaa gttcagctag aataacagat tcattttatc aaactgtact atatgcaagt
    18961 gctccaacag ctgcattagg aggaacaaac ttaggacaag taggtgtagg tagtaagata
    19021 tcaagtataa ataaagatat ggtacaagga aatgtacaac caactaatag accatctgat
    19081 ttaatgatag atggagatgg atatcttcca gtagtaagac aaggaacagt tatgtatacg
    19141 agagatggtt cttttaattt agatacagga tttaaagcag ctgatggtgc tttgaagaat
    19201 attaaaggtg gaagacttgt aaatggtgat ggatatatgt tacaaggtat gatttatgat
    19261 ggggaatata aagctgatac tggagaattt gttaaaaagg gtgatgctaa aggaaaagag
    19321 cctattgtaa taccacttca aagtataagt cctaaggatg gaacagttca aaatgtacta
    19381 gagtataata tatcaaagga tggaacagta gaatttttat tgagtgatgg tcaaagaact
    19441 caatttgtct atgatatacc tgatggtgct aaggtaggag atacacctgc tggaatagat
    19501 aaattaaaag gtacaataca aaaagtgcaa acatatgcat tccaaaatcc agcaggacta
    19561 gatgcagaag gaggaaactt gtttaaacca tcagcaaact caggagaccc acaaatagca
    19621 ggagctacag gaacaataat acaaggggca atagaagctt ccaatgtaga tatagcagaa
    19681 gaatttacag aaatgataat agcaagtagg tctttccaag caaacagtaa aataatatcg
    19741 acatcagacg agatacttca agaaataata aatcttaaga gatagtaggt acatattatg
    19801 ataagagtaa cggatttaaa cggaagaaaa tacatattaa attcagacct tatagtgagg
    19861 attgattctg taccagaaag taggattctc cttacaacag gagaaaaaaa ccttgtaaaa
    19921 gagtcggtag atgagatagt agataaagta ataaagtata aaagaaaaat aaatgtaggt
    19981 tatattagga gcgaaaaata atgaaaaaaa cagattcact gacatttatt ggactaatag
    20041 tatctacagt attagtttta gttggtgccg caaaaggaag tagttcaggg ttaaagaatt
    20101 tttttgatgt ttcatcaata ttaatcacag tgcttggttc atttggagct ctaatgatta
    20161 catttactat agatgatata aaacttataa aaaatgcatt acaatactca tttaagacaa
    20221 tgagtgtatc aaagttagat ttactagaac aatttaaaac actttctaaa aaagctagaa
    20281 aagaaggtct tttatcaata gagagtgatg taatgggaat agaagatcca tttatgaaga
    20341 aagggttaga gctgtgtatt gatggattgg aaatcaatga gataaggtcc atcttagaac
    20401 tagagataga ttctgttgaa gatacaaata acagatactc taaactattt aaaacttggg
    20461 gaacctttgc acctgctttt gggatgttgg gaacactaat aggtttaata caaatgttag
    20521 ctgaccttac atctccagat ttaatagcat ctggcatggg aaaggcatta attacaacgt
    20581 tttatggttc tttacttgcc aatatcgctt tgaacccaat tgcatataat atagatgaga
    20641 agactgaaaa agaaatttat gtaaaggaaa tgatgttgga aggtataata agcatacaat
    20701 ctggggagag cagtatagta gttgaggaac gtttagccac atacctttca aacaatgaga
    20761 agttagaaat tatgaaatcc aataaaaata cagaaagagc aatgagcaat ggcgcgtaaa
    20821 aaaaataaga aaaaggatga gataaatcca gatgcatggc tggctactta tgcagatact
    20881 ataacactga ttcttacatt tttcgtatta ttgtatgcaa catcagttgt agatatggaa
    20941 aaatttaaaa acatagcaga tgcgttaaaa ggtcagtttt ctgggacaag tatatttagt
    21001 acgggttcaa gtacaagtga gaaatctcca atagacagtg ttttagtgga taaaaatgat
    21061 aaatcaaatt tagaacagga tttaaaagat aaagttaata attctaactt acaaaatagt
    21121 gtaacagtca aagatgatag tagagggatt ttactagagt tagatgattc aattttattt
    21181 gactctggga tttctgagtt aaaagtagat agtaaagcta ctcttaataa agtatatgac
    21241 atgataaaga tgatgaaaaa taagatagtt atagaaggcc atactgataa tgtacctgta
    21301 aaatcaagta cacatgaatc caattgggat ttatcttcta gtagagcagt aagtgttgta
    21361 agatactttg tagaagaaaa gggcatggac cctagactat tttcggcaac aggatgtgga
    21421 gagtatcaac ctttggtttc aaatgaaaca gcaaagggaa gggctgagaa tagaagagtt
    21481 aatatactta taactattga ctaggaggaa gaggtttgtt caaaaataaa aaaattatta
    21541 taattatttt gatactgatt attttctcag cgggagcagg tttttttata ttaggagaag
    21601 gtggaaataa acaagttgac aagattgcag gtttgtttat tccaacagga aaaggtaaag
    21661 atgaagtaaa gaccgaaaat cttgacttag aagagatggt attaaagcta gctgatgaag
    21721 gtgtaagata tttaaaagta tcaataactg tatcttatga ttcttcatat aaagatatag
    21781 aaaaaaatac gcctttaatt agggataata ttataaattg ttttatggca aaaagggcag
    21841 atgattttac tgctgataat ttaaagaaca taaaagaaga aattaaaaat acattaaata
    21901 tacaactagg tgaaaatgta attcaaaata tatatttcac aaatatagta gtgcaataaa
    21961 aggatggtaa gacatgatac tttatttact aaaaatatta atatgtatcc caataatttt
    22021 aatattgata gtcttatctt taaaaattag ccaaggaact ttattaaaaa aatataatga
    22081 taaatatgta aaaatactag atattgtaaa tataagtaaa aacaatagca taataatttt
    22141 aaaaataggt gaagaaggat gcattatggc atcttcttct tctagcatga gtaaaataaa
    22201 agatttgacc aaagaagaaa tattaaaaat agaaaacgac attaaagaaa gaaattttaa
    22261 aattcaagat tttaaaatgg aaagaattaa aaagaaatat gatttcagta gatatattgg
    22321 aaaatttaca aaaaaggaag ataagtaaat ggaactatta tcaaaattac ctttggctga
    22381 tgtaccatat acaagttcaa tgcaattgtt tttatttcta actgcattaa tgtttttacc
    22441 atttgtaatg ttcatgatga caagctttgt cagaatagtt ataagtttat catttttgaa
    22501 atcagcatta ggagcacagc aagcaattcc aagtcaaatt ttggtaggac ttgcaatagt
    22561 attgacaata tttataatga gaccagtatt aaatgagata aacgaaaagg cattacaacc
    22621 atatatgaaa gaagaagtta ctatggaaga agctatgaag gaagcagaag gacctattaa
    22681 agagttttta ttaacacaaa ccagacagac agatttagat ttatttgtag aacaggctgg
    22741 tttaaaagaa aagaaattaa ctcgtgaaaa tataccacta tctgttgtag ttccagcatt
    22801 tgctataagc gaactaaaaa ctgcttttca aataggtttt ttaatatata taccattttt
    22861 gataatagac cttgttgtgg caagtgtttt gatgtctatg ggtatgttta tgttgcctcc
    22921 agtcatgata tcattaccat ttaagttatt attatttgta atggtggatg gatggaactt
    22981 gatagtaaaa acattgatat tgggatttgg ataggagaaa aatagtatga gtgaatttat
    23041 gataatgagt gttcttaaaa atggcatgtt tacaggagct atgattgcag ctccagtact
    23101 cattgtttct cttgtagttg ggcttttaat aagtgtgttt caagctacaa ctcaaatcca
    23161 ggagcaaact ttgacttttg taccaaaact aatagctatt cttttagtag tagctattgg
    23221 tggaagctgg atgttacata ctcttattag ctttacttct gagatattca atatgatatc
    23281 tcttataact aaataagtag gatacatatg atttttgtat ttattagaat agtagcattc
    23341 tttggaacac taagaatact atttccagct ggtacacctg ctttatttaa gtcactcttt
    23401 gcaatcatta tatcaatatt gatttcaagt actatgcaaa ttgaatatgc aacaaatata
    23461 gacaacattg tattgtttac cttatatggt ataaatgaga ccataactgg gattatgctt
    23521 ggatatataa ccaacctatg tttttatagt atcagaatgg cgggttctat gatggaccaa
    23581 cagatgggat tatcaatgat taatatgttt gacccaaatt caatgactca gacaacttta
    23641 attgataatc ttatgaactg gacagcactt atgatatttt ttagcatgga tggtcatcat
    23701 gttttaatta gaggtataag gtatagtttt gagttaattc caataggaaa accatttgta
    23761 gataataaca tagattatat aataaacata tttgtgcagt gctttttaac tggattcaaa
    23821 atagcaatac ctattgtact ttgtctgctt atggcagatt ttattttagg gcttatatca
    23881 agaagtgtac ctcaattaaa tgtaatgata gttggtatgc cattaaaaat attggttggt
    23941 attgcattat ttatcatatc aataccttta attgtcaatc aaataagtca tttattaagt
    24001 caaataccaa agatgtatga gggaactttt gcactagctc ctatgttttt tatgggttca
    24061 acagataaaa cagaagaagc tactccaaag aaaaagggag aacaaagaaa gaaaggtaat
    24121 atagcaaaga gtagagaatt acccgttgca atgactctac ttgcattcac cttattagta
    24181 cctacattat tttcatatgt agtagataca cttaaatctt cgcttaatta ttttttgagt
    24241 ctagattttt atatgaacat aaattattca aacctagaga aattagttat agcaggatta
    24301 atggattttt ttaagatatt tttaccaata gctattccct ttttagtttt aggcataata
    24361 gcaaacctat ttcaggtggg aatactgttt actggtgaaa ctttaaaacc aaatttatca
    24421 aaattaaatc cagttagtgg attcaaaaac atgttttcta tgagaagttt gtcaacctta
    24481 ataaaggata tagcaattat atctattttg gcttatatag gatatacatt cttccaagat
    24541 aattatttag atatattaaa gctaggtaac atatacctac caactttaat gtacacagtt
    24601 aaagatttag tatattcaat attgagtaag atttgtgtag ctatgatagc tatagcagtt
    24661 gcagattatg tttatcaaag atacagtcat aaaaagcaac tgagaatgac aaaacaagaa
    24721 gttaaagatg aatataaaaa ctcagaagga gacccagaag taaaagctaa gataaaacaa
    24781 aaacaaagac aaatttcgtc tcaaaggaca atgcaagcag taccaagtgc tactgtaata
    24841 gtaaccaatc caacccactt atctatagca gtgagatatg aaaaagggaa agaccaagct
    24901 ccagtagtgg tggcaaaagg tgcagattat ttagctttta aaataaggga aattgcaaag
    24961 ggaaatgaca tccctataat agaaaacaaa cctattgcta ggttgttata taagcaagta
    25021 gaaatagacc aagaaattcc agaggacatg tatcaagctt ttgcagagat attagtggca
    25081 gtatataaaa tcaaaaatag atacaaagtc cccaaaaggt aggataagta atgaataagt
    25141 ttaatttaaa ggaaaatatg gacgtaatag tggcatttgg tgttgtaggg attgttctta
    25201 tgataatagt gccattacct acatttatat tagatatttt actggcaatt aacataagtt
    25261 tatctgtgct aattttacta atgacattat ttacaacaaa tgtacttgat ttatctatat
    25321 ttccaacagt gttacttgtg acaacactat ttagattggg attaaatctt tcatcaacta
    25381 gattgatttt aacacaggga tatgcaggtg aagttataga ggcatttgga agttttgttg
    25441 taggtggaaa ttacgtagta gggataatta tattcttaat tatattaatt gttcaatttg
    25501 ttgttataac caatggttct ggaagagtat ccgaagtaac agctagattt acattagatg
    25561 ctatgccagg taagcagatg agtatagatg cagatttaaa tgcaggagtt atagatgaca
    25621 aaactgctag aaaaagaaga aaagagcttc aacaagaagc agatttttat ggttctatgg
    25681 atggagcaaa taagtttgta aagggagatg ccatagcagg tcttatagta acggcaataa
    25741 acataatagg tggaattgta ataggagctg taatgctgaa tatgactctt atgggtgcag
    25801 cacagacata tgtaagactt actgtaggag atggtcttgt aagtcagata cctgctctat
    25861 taatatctac atcagcaggt ataatcgtaa caagagcttc tactgatgaa aactttggtg
    25921 gattagtagg taaacagtta acagcaatac caaaagcagt aatgatgact ggctgtgttc
    25981 taatcgtact tggtatgatg cctggattac ctaaaccagc atttttttca ctaggtatag
    26041 gtgctatagc agcaggttac tttttaggaa gagaaaagaa agatacttta gaagacttgg
    26101 agtttgaaaa acctgatatg gtaaatccag ataaaaatga agtagaagat gtaacaaact
    26161 taataaatgt agaatcaata gaagtagaaa ttggatatgg tttaatatct ttagctgatg
    26221 aatcagcagg aggagattta cttcagagaa tagcatcaat aagaagacaa tgtgcaatag
    26281 acatgggtat agtcgtacaa cctataagaa taagagataa tttacagtta aatccaaacc
    26341 aatacaatat aaaaataaaa gggaatgtag ttggtgggta tgagataatg cctaatatgg
    26401 ttttatgtat ggatcctatg aatcaaggat ttagtatatt tggaataaag acaattgagc
    26461 ctacttttgg aatagaggca ctttggatag aaagtgacaa ggtagaagat gcagagttaa
    26521 agggttatac agttgtagat tcatccacaa tacttataac tcacttacta gatataataa
    26581 aatcaaatgc acacgaatta ctgggaagac aagaagttaa gactattata gatgcagcaa
    26641 gagaaaatta tagtgctgta gtagatgaat tgataccaga tttgatgaca cttggtgaaa
    26701 ttcaaaaagt acttaaaaat ctgttaagag aaaaagtaaa tataaaggat agagttacaa
    26761 tacttgaaac gcttgcagac cagtctagaa atacaaaaga tatagagctt cttactgaat
    26821 atgtaagaat agccttggct agaagtattt gtaataattt agttgatgat gataagacaa
    26881 ttgtagtggc tacattgtct ccagaaactg aagagttgat atctaataat ttacagaggt
    26941 ctgtaaatgg aacttatcca gcagttgacc cagaaaatac aaataaaata tttgaaagta
    27001 tacaagaagt aatgagcaat gtatatttca atcataatat accagtaata gtagtatcac
    27061 caaagataag agctccattt agaaaattag ttgaaattgt atatcctaat ttgactgtat
    27121 tatcattaaa tgaaatacct aacgatatta aaatcaaagc agaaggtgtg gtgagcatat
    27181 atgatgacga aaaagtatac agctaacaca attcaggatg ctatgaatct agccaaaatg
    27241 gaattgggtg acaatattac attaatagat aaaaaagaag taagaaagtc tggaatacaa
    27301 ggtatctttt caaagaaaga tattgaacta actattggat gggaaaagag agaaaacata
    27361 gaacaaaaag atttaaaacg agagatagaa caattaaagt taattattaa caatatgggg
    27421 tttgataata agaatgataa tgatatagat aaaatatgta aaaatctact aagtttagaa
    27481 ttgaatgaag aaattgtaga atctataaga attgatttgc aagaaatgaa gttcaatgga
    27541 atcgatacaa gtaaaaattt agtagaaatt ttaaagaaaa agattaaaat agaaaatcaa
    27601 gtaataaatg gtaaaattgc tctagtcgga ccaccaggag taggtaaaac gactacaata
    27661 gcaaagttag ccgcaaaatt agtttttgaa gaaaataaaa aagtaggagt aataactata
    27721 gatacataca ggattggtgc tgtagaacag ttgaagatat atactgatat aatgaatatt
    27781 ccctttaaag gggtaataag tcctgatgaa atggaattgg cacttgacga gatgaaagat
    27841 tgtgatgttg tattaataga tacaacaggt agaggctata aaaactctat gcaaatacta
    27901 gaaatcaaaa atcttataga taaagctgaa actgataata tacatttagt tttgaattgt
    27961 acaacaagag aaagtgatac aaaagcaatt atagattcat atagaaatgt gaattttaaa
    28021 agcttaataa ttacaaagct agatgaaaca ataacatatg gatcaatatt taacataatg
    28081 aattatgcac aaaaaccaat atcatatatt acaacaggac aaaatgttcc tgatgatatt
    28141 ataaaaccta atgaagataa gattataagg ttactattag gagttgaaag tatatgatag
    28201 accaagctca aattttgaga aaaatggcaa tggaaaaaag aggacttgat gagtttatag
    28261 tagagaatga gaatgtacct aagataatta caatagcttc tggtaaaggc ggtgttggaa
    28321 agagcaatct cgctacaaat ttgtctatat gcctgaccaa acttaataaa aaagttctta
    28381 tattagatgc agacatagga atgtctaata tagatataat aatgggggta aatgtaaaag
    28441 ggactataat tgatgtaatt aatggcgaaa aaaatataga agatataatt tcacagacta
    28501 agtatggtgt aaatataata tctggaggtt ctgcccttaa tcatatagaa gactttacag
    28561 aagctcaaag aaataagttt atacatagca tagaacagat tcacgatgtg gattttataa
    28621 taattgatac aggagctggt atgagcaaaa gcttattgtc atttatatac tgtagtactg
    28681 aatttttctt aataacaact ccagaaccga catcattgac tgacgcatat agtttgttaa
    28741 aagctataag taattttggc ataaagagaa gtgcaaatat aataatcaat agggtaattg
    28801 atttagaaga agcaaaatct acatacaaaa gaataaaaat ggttgtggac aaatttctaa
    28861 accttaatct agaattgtat ggatatataa tagatgacaa aaaagtaagt ttatgtgtaa
    28921 aaaaacagat accattcatt ttagaatatc ctacaagtat tgcatcaaaa tgtattatag
    28981 ccatagcaaa gagaatggta agtcagtcaa gagaagatat tgttgataat aaaagttttc
    29041 taagaaagat gtttagtata tttaaaagct aaggaggcgt agttaatgaa tagagaagaa
    29101 ttaataaaag agaatatgcc tcttgtaaag agtatagcaa gaaaattttt tataccaggg
    29161 aaaggttttg aatatcaaga cttagtaaat agtggagtca ttggattgat tgatgcaata
    29221 aataaatttg atgctgaaaa aggggcaaag ttttctagtt actcttatat aaaaataaaa
    29281 tctgctatat tagatgaaat tagaaatcaa agtccaattt ctaaacataa tctaactaaa
    29341 gtaaataagt ataatagagt ggtagaaaag ttgcaatcta aattattaag agagccaact
    29401 tcatatgaaa ttgcaaaaga attaaaggtt tcagaaaaag aacttcatga tatcgaaagt
    29461 aatatagata tgttaaatat tgtatcatta aactatgtaa tctttgaaga tacaaatgaa
    29521 actgtacaag atgtaataag tgatagagaa gaggaagctc cagaaaatat aatagaagaa
    29581 gaagaaaagt tagagattct atccaaagca attagtaatt taaatgaaag agaaaaatta
    29641 atactttcac tgtactatta tgaagattta aatctaaagg agatagggaa agtgctaggt
    29701 gtttcagaat caagagtttc acaactccac agaaaatcaa taagaaactt aagaaacaag
    29761 ataaaagaac ttaaatattc tatatagatg gtgattgata agatgataca aattatatta
    29821 atagtagtgt ctattttaat aatagtaact atagttataa atttacataa ggaatcaaaa
    29881 aataaagata acagatactt tgataaaata tttgagcaag aatatgaagc aaatgataga
    29941 ttaataattg cagaaaaaag aagattcttt ttagacaaag tacttgcaga aaaaagaatt
    30001 aatagtcata taaaagttta tgaaaattat aacctaataa aggctaatga agaaagaaag
    30061 gaaaacatat ttatagaatc aaaaaaggaa gtaagacaag aaaatgagtt atctcttaaa
    30121 gtgaaacatc ttataagttc aggttatgat gatgtggaaa tatgtaagat gctagatatt
    30181 ggaaaggggg aactgttatt aataaggagt tgttacaaaa tataaggata ttattatgtg
    30241 ataaaaaagt attcttagga ataggtattg gtatattaat atcaacttta tgtataatgc
    30301 tttttagtaa taatgatatg agtaaggcag agatagaaaa aaaggccaga gcgttaggca
    30361 tgaaatatac aagtgagatg aaagttttag aatagatgat attgtttaga catttacaaa
    30421 aaaggaggtc ataatgtgat aagaggatta tatacagcag tttctgctat gataactaat
    30481 caacaaaagc aaaatgttgt tgtcaataac ctagctaata tggatacaaa tggatataaa
    30541 agtaaacaac ttttgactaa aagttttgat gaattaaagt tggagggtta caataactac
    30601 gcaaatggaa ctaaacaaaa gcagattata ggcgatataa gtccaggtat ttctatggat
    30661 gaaactatca caaattttaa tcaaggacct ataaaaacta ctgataataa aatggatgta
    30721 gctattcaag gtaagggatt ttttctagtt agtgatgcag ctggaaatca attttacact
    30781 agaaatggga acttcagaac agacaatcaa ggtgacctta taacaagtga agggtattat
    30841 gtacttggga cgaatactgc tactggtgct gtaggtcata taaatgtagg aaatcaaaaa
    30901 tttgaagtgg caaaagataa tacaatatca ctagacggaa atgctatgta taaatttaat
    30961 attgtagatt ttaataacta caataactta aaaaaagaag gagataactt atattctgga
    31021 ggaggagcta tgaaagtaaa taatgcactt acaatacaaa gtgcgataga gtcctctaat
    31081 gtagatatgg tatctgaaat taataatatg atgacaatat caagagaata tgaggcaaat
    31141 caaaagataa ttcaggctat ggattctaaa ttagcaaaaa tagcaagtga gattggttca
    31201 gtaagatagg taataggagg tgaattttta tgtacacaat gttttatact agtaaaacag
    31261 ctttatcagc aaatcaaagt aaactagata taatatctaa caatatagca aattctgatt
    31321 caacaggata taaaaaaata aatatggaat attctgattt agtggatgaa gtactaaata
    31381 gaccatcata tcctacaaat ggcaatgata tatcgactgg aacaggagct aaggcaacag
    31441 ctcccatgag aaattatgaa caaggtgcat tggctcctac agatagtaag agtaatttgg
    31501 caatagatgg agaaggattc tttagagtga taagaaaaga tggtacatat gcatatacaa
    31561 gaaatggtgg atttaatgta gatgctttag gtaagattgt agatgataat ggaaatatat
    31621 tagatgtcca atttgatgca ggatataact ataataatac aaatatagat agtacaaacc
    31681 tgacagtttc aagaaatgga gagctattcg tagataataa aaaaatagga aaaatcaact
    31741 tatatcaacc tataggaact caaaatttta tatctgaagg agatagttta ttcgtcgcaa
    31801 ctgatgcagc acagattaaa caagttgaga aagtcgatat agttcaaggc tatagagaga
    31861 ggtcaaatgt ttctttacaa gaagaatttg ttgacttaat agccactcaa agagcatttc
    31921 aaatgaactc aaagggaata aagacagcag atgaaatgtg gcaaatagcg aataatttaa
    31981 gagctaaata attatgtaag gtgaggaata ttcatgaatg atatgaaaga gtacgatttt
    32041 ggcaaacctc agagttattc aagagaaaat ttaacttgtt taaatttttt attagctgaa
    32101 ttttgtaaaa aatataaaaa ctatataatt tatgaactta agtgtaattc tagtatggtt
    32161 gttgatagta tagaccaagt aaattatcag acttttttag atagagtaac ttcttcatgc
    32221 gtagtagtac aaaatgcaat ggaacctgtt atgaaaaact tttcttttag aatagataga
    32281 agtgtagctg atatgtggat tgatataact tctggaggaa ctggagttgt aaaggataat
    32341 gatagagaac ttacagaact tgataaaaaa gtgcttttac accttatgga tgatttagta
    32401 agaaatatgc acttatttga gggttttgaa aatatacaag tattggatac acatacacat
    32461 attaatttgc cacaattatg ttctccaact gcaccagtat gcgttgtaga tttaaaagtg
    32521 ctaaatgata atgagtatat tggaagtgtt tctctttgct ttccatacaa tggtttagag
    32581 gctctgttag aaagtatatc tgttatagaa ttttttgata atgatgtagg agatgattca
    32641 gaggaattta cccaaaaaat ctataatagt gtatctaata tagaattatc agttattgca
    32701 gagataggaa aagtatcaat taatgttgag gatttactca aactagaagt tggagatgta
    32761 atagtaacaa ataaaaaaat aaatgattat atagatatat ttgtagaaaa ttcaaaatca
    32821 tacacagcaa caccaggatt tatatcaagt aaaaaaggtg taaaaataaa tgatgctgtt
    32881 ggaaaagagg tgtagttatg gacaatagta atcttatttc agatgaagaa gcaaagatgt
    32941 tattaaatga taaagttgaa agtgaagttg atggagatga tgagttagcc ttagatgtct
    33001 ctgaagttaa tcgaaataat atacaaagga tacttgacct taagttagaa ttatctgtag
    33061 ttataggaag aactaagatg actctaagag atattttaaa tttacataaa ggttctctta
    33121 ttgaactgga tactttggca gaccaagatg tggaaatact aatagataat aaggttttgg
    33181 cttatggaaa agtagtggtg gtagatttaa attttggtgt aaagataaca agtatagtaa
    33241 gtgaagaaga catggtaaaa tctcttgttt agagataaca tcaattaata cttaaggcat
    33301 gaggtggtac ttattttaaa cttaaatgat ataaaaaaag atatactaaa actcaataaa
    33361 aaagactcat gttctctata taatgaatct attaataata taaataataa ttgtatatca
    33421 actggattaa aatttttaaa acaagcaaga gatttaaacc cagatgatgt tgatatatta
    33481 aatctcacag gtcttgtaaa cttactaaaa tgtaattttg atgatgctgt agaaagtttc
    33541 tataagagtt cactttgtga aaaaactgct ctgtgtgaaa agtatataaa catgcttaca
    33601 tctgaggagt ttttaatttt ctttgaaaag tacaatcaac ttataaggtt aataaataaa
    33661 ggaatgtata aagaagctat acaagggttt aaacttataa ctgaccaata ctgtgattta
    33721 atagaacctt atgagttgct tgccttactg tatgacaagg aagaagaata tataaaattt
    33781 gatgaatgtt tagaagtatt gaagattatt gataaggaaa attatttatt aaatcaagaa
    33841 aataacacat tatatatgta taatagtgct aatatacaaa ctaaaagtaa tataaaagta
    33901 gatgattata agaatgagtt cttacataaa agtagtagta accataaaat taatgattat
    33961 aatgatgtaa ctaaagaaaa gagttcaagc aaaaaccaga aaagttcaga ttctaataag
    34021 aaaaaaagta ttaaaaaaaa taagtaccta tatatagcca taatattagg aatattatta
    34081 gtaggacaaa gtatatatag ttcttataag atgaataatt taactgataa aatatctaaa
    34141 aataatgaaa ctaaaaatga agagtcaata gattctaact tgactaagaa agaaaataat
    34201 atagaagaaa atgaagatat gagtaaggct tctaatataa ataagagtaa taaaaacatt
    34261 aataaagagg atgatgtaaa atctaaaaaa aatactgtag aaaatgaaga tgataaagat
    34321 tctgacttat ttacagagga tgaattgatg tctaaggcga atactttaaa atcagaaaag
    34381 aaaactaaat cttctataca actatataag aaagttgcag atataggtga aaataaaggg
    34441 aatacatctg aagcaactta tcaagttgct attctcagtg aaaagttaaa agactataaa
    34501 actgctgaag aatattataa aatgtatgta gaaaattact cagaaaaaga tgcttatttt
    34561 gatgaatctt attataattt aggtatgatg tactataaca atggtgattt aaaaaattct
    34621 aaattgacat taaagaagtt ggttaataag gtaccaaata gtatgtataa taattcaaaa
    34681 gtaaaagaaa ttttaaaaga agaatagtta aattagataa gaaaaataaa ttattttatt
    34741 ttaaaataat atgattataa tatattgaca taccatttta aaaaatttaa aatattaatt
    34801 ataaaatgag gagggataaa aaatggaatt tgaaaaaggg agtatttcaa ttcatacaga
    34861 aaatattttt cctataatta aaaaatggtt atactctgac aaagacatat ttataagaga
    34921 gcttataagt aatggatgtg atgcagtaag taaacataaa agacttgtat cactaggaga
    34981 aatatcagaa aataagtcat cagattataa aattacagta tctgtaaata aaggagaagg
    35041 tacacttaaa tttattgaca atggaattgg tatgactgaa gaagagataa aaaaatacat
    35101 aaatcaagtt gctttttcag gtgctgaaga tttctttaat aaatataagg ataaaatgga
    35161 agagtcaaat gatataatag gtcattttgg actaggattt tattctgcat ttatggtgtc
    35221 taaaaaggta caaatagata cactttcata tacagaagga gcgactcctg taagatggat
    35281 atctgaaggt ggtacagaat acgaaatatc tgaatcagat gctagaaatg atagaggaac
    35341 tactataaca ctatttattg atgatgatag taaagaattt ttagatgaat ttactgtaag
    35401 aggaataata aataaatact gttctttctt accagtagaa atatatttag aagatgttga
    35461 aagattagaa agagaagcta aagaagcaga agaaaaagct aaaaaacaaa aggaagatgg
    35521 aaaagaagag attgtagatg caaaagtaat tgagccttta aacgacacta atcctctatg
    35581 gttaaaatct ccaaaagact gtactgatga ggagtataaa gagttttata gaaaggtatt
    35641 caatgtattt gatgaacctt tattttggat acatttaaat gtagattatc ctttcaattt
    35701 aaagggtata ctttacttcc ctaaattaaa aaatgagttt gaactgactg aaggaaaagt
    35761 aaaattatac aacaatcaag tgtttgtggc tgacaatata aaagaagtta taccagaatt
    35821 tttactttta ttaaaaggag ttatagactg cccagactta ccattaaatg tatcaagaag
    35881 tttcttacaa aatgacaggg atgtaagcaa aatttctaaa catataatta aaaaagtagc
    35941 tgataagttg aaatctcttt gtaaaaatga aagagaagaa tacaataagt tttgggatga
    36001 tatacaaata tttattaaat atggttgctt aaaagatgaa agtttctatg agaaggtaaa
    36061 agaatgtata ttattcaaaa ctattgatga tgagtatata acattacaag attatttaga
    36121 aaaatgtaag gacaaacatg aaaataaggt attttatgta agtgataagg aacagcaatc
    36181 tcaatacatt aaactattta aagaatacga cttaagtgct gttgttttaa actcatcaat
    36241 agatactcat tttatttcat ttatggaata taaagagaat ggagttaagt ttaatagaat
    36301 agatgcagac ctttctgatg tattaaaaga taaaaatgaa aataaagata gtgaagaaaa
    36361 caaagaagag atagctaaga tagaaggttt gtttaaagaa gctgtcggag aaagagtgaa
    36421 gaattattct gtagaaggat tgaaaaatga agacactcca gctatggtat tagtatcaga
    36481 acaatctatt agaatggcag aaatgcagtc aagatttgct ggaatggatt taggaatgaa
    36541 ttttgaggaa gaaaaaactc ttgtaataaa tgagaacagt ccaataatca aaaaattggt
    36601 ttctcttaaa gatgatgagg aaaagaaaga taagattacg cttatttgta accagatagc
    36661 agatttagct ttactttcta ataaagaatt aaagccagat gagttagatt catttgtaca
    36721 aagaagtaat aagcttatga gtcttttgat agaattgtaa gattgaattt gaatacattg
    36781 aagcaaattt ataaaaagta ttcaatatca gtgatgagaa agaagtgtct caaaatgaac
    36841 ttttcagttc atgaggcgct ttttttatgt gaaattaaat ttattttaat catttcaaca
    36901 atgaaaaata tatttatctc tattttgaga cagaccgttc tttatttttt tatctttggt
    36961 taatttttaa atatttaagt aggttctaat aaaaaactct atttagttaa atatagttaa
    37021 cttgatatat aataagataa tacatataat taagtaaaag attgtttttt agtttatgtt
    37081 taaatacata attacacttt gttaaaaaca taaaattatt actaaagtag aaataggagg
    37141 ttttagcatg aattttaatt ataatttgcc agtaaatatc ttatttggga gaggcagaat
    37201 taatgatgtg ggaaaagaag tatctaaata tggcaaaaaa gtattgatag taacaggtaa
    37261 aaatagtaca aagaaaactg gattacttga taaaacaata gatttactaa aagactctaa
    37321 aatagaatat gaagtttttg atagagtaga acaaaatcct cttataacta ctatatattc
    37381 aggagtagaa attataaaat caactggatg tgattgtgtt ttaggtcttg gtggtggaag
    37441 cattatggat gctgccaaga gtatagcttt ttcttataaa aacccaggag atttaaatga
    37501 ttatatattt ggaattagac aaggtgaaga agctttacct ataatcttag ttccaacaac
    37561 atcaggtaca ggtagtgaag gaaattgttt tgcagtactt acaaattcag aaactaaaga
    37621 taaaaaatct ataaagaaga atagtatgta tgcaaaagca tcaataattg acccagaact
    37681 tatggttaca atgccaaaac atataattgc atcagttgga tttgatgcac ttgcccataa
    37741 tatggaagca tatctttcta atggaagaaa ccctcttgca gatgtacaag ctatttatgg
    37801 gattgaactt atatcagaaa acctaataaa ggtatataat gatgttaatg atttagaagc
    37861 atgggaaaaa ataacacttg caagtactat tggaggaatg acgattggta cagcgggtac
    37921 agccttgcca catggaatgg agcatcctgt aagtggtctt agagatgtaa tgcatggaaa
    37981 aggacttgca gctttgactc ctactattat agaaagttca tgggatagtg atattgaaag
    38041 atttggtaat atttcaaagg tattaggagg aacttgtgca gaagattgtt cagatgcaat
    38101 tagaaatttc cttaaaaaaa tagatttaga tataaaacta agtgatttag gtattgaaaa
    38161 atcagatata gattggttaa ctgataattg tatgaaaata tcagttgcaa atattaagag
    38221 acatccaaag tattttaata aggaacaaat aaaggaaatg taccataagt ctttataatt
    38281 tgactttaca tatttagttt tatataaaaa ttataaatta ataataaaaa attataagta
    38341 attaatagta aaaaaattgt gtatagaagt tatatatata ataaatttgt cttaagcttt
    38401 tattttactt tctaataaat aaaggagttt aaaaaaggga agattaatta agtaatattt
    38461 tataaaattc ttcgatttaa aaactttaag tagaggtgaa ataaatgttt aatcccaaaa
    38521 gaataatatt cgaaaaagga actttagaca ctgatgtggg taaaaaaata tacaataaat
    38581 ttagagataa taaagatgta gagataatta atatatcatc aaataggatt aaagaacata
    38641 ttccaggtga aaatctatat gaacaatata gaagtgggaa gcagactatg gtagttggga
    38701 ttagaaaaag tctgaagttt caaacatgta aaccttctgc taattatcaa ctaccacttg
    38761 tcagtggatg tatgggaaga tgtgagtatt gttatttgaa cacacaatta ggtgataaac
    38821 catttataag ggtgtttgta aatgtagatg aaatacttga acagacaaaa aaatatattg
    38881 aagatagaaa gccacaggta accatatttg aaggggcagc tacatcagac ccagttccag
    38941 tagagccata tactaattct ttaaagaaaa caatagagtt ttttggaaga gaggaaaaag
    39001 gaagatttag atttgttact aaatacagtg atgtggatag cttattggat gctgaacata
    39061 ataatcatac agaaatacga tttagtatca atactcctag aattataaat gaacatgagc
    39121 attatacagc ctctgcaaat aaaagaatag agtcagctgt aaaacttgct agggctggtt
    39181 atcagattgg tttcataata gcacctgtgt ttttatatga aggttggaaa gaagagtata
    39241 aaaatctgat tataagtata aaagaagctt taccaaagga ttttgataaa aaaattatat
    39301 ttgaagttat atctcataga tatacaacta aagcaaaaaa tagaatacta gagatttatc
    39361 cagatacact attgccaatg gaaaatgagg atagaaagtt taaatacggt caatttggat
    39421 atggtaagta cgtgtatgat aaagaacaat tagatgatat gaaagagttc tttcttaagg
    39481 aaataaatgg agtatttaaa aatagtttaa taaaatatat aatatagcaa atcttaccta
    39541 aagatttgct atattttatt tttatacatt ttatgtttat aataaagcaa tgagatttag
    39601 cactatcata taagaaaatt aaaaagggca ctatctatca agaacatttt ttctagtttt
    39661 gatactataa aggataagag taacaaaaat atcaggagga gttatagtat gcatgcatgg
    39721 gagtctattc aaattacatt ggatttaata gaagcaaatt tatcagaaga aatatcaatt
    39781 aatgaattag caaataaagc aaatttatca ccattttact atcaaagact atttaaaaga
    39841 ttggtaaata agactgtaat ggagtatata aagcttcgtc gtcttgcaag agcttctgaa
    39901 tacttagcag aacataagaa tcgtatactt gatgtagctt taaattttgg atttgctagt
    39961 catgagacat ttacacgttc ctttaaaaaa gcatatggat taactccaga aaaatataga
    40021 gcaaatccag taaaactcaa tcactttatt aagccagaat taattttaaa ttatgctatg
    40081 gttgatgaag gtgttccact gattgcagat gatatagtga ttgaagtcaa tcgtaagaca
    40141 ttgagtaatc caaggacatt tgttggaata gaaatagaag tacctatttg ccagttggtg
    40201 ggcggtgaaa caactggaat tgcaatagta gaggaattat ggatgaaact tggaagtcaa
    40261 agacataata taccaaatca aattccaggt ggaaatgaat ttgctgctct ttatatggga
    40321 aatgcaaaag aaggaaattg tatgtatatg gcaggtgtag aggtagagtc aggtacatca
    40381 gtagaaggat atagtacttt tgagttgcca gctaaagaat atttagtatg tggatttgag
    40441 gcagaaaact ttaatgagtt agttaattca gctgttttta aagcagataa atttatggaa
    40501 agatggatga agaaacataa tttaacaact actgattttg caattgagat gtattacccc
    40561 actacacctg aagcagcata tctggaacat tggattgttc cggttcctat tgaacaatag
    40621 aaaggatgat gaatatgaat tggcaagatg cgttttttca agatactaaa cctaaatttg
    40681 agcaaattaa tgaatttatt aataatccat tatgggacaa gttaaataaa actttggttt
    40741 ctacttataa tgtagaacct aagcttgaat acagtaaatg ttcaatgcaa agaggttgga
    40801 atgtaaaata taaaaaaaga ggtaaatcac tatgtacttt atatccacaa gaaggttatt
    40861 ttaaagcttt ggttattgta agtgaaagca atagagtaga ggttgattta tttattaata
    40921 cttgttgtga ttatatcaaa aaaatatata atgaggtaaa cttttttaat ggaagtaaat
    40981 ggctaatgat tcaagtagat agtttattgg tattaaacga tatgcttgaa ctaattaaat
    41041 ttagagctta acatatagaa caggatgaat atataagtca tcctgttttt atattcaatc
    41101 agataaatta atatatagtc tataatatga ataatttagt aaatttaaat ttactttatc
    41161 tttctacatt ctttgcaact aagtccacat gtgttttttt cttgcatatt agtagcaccc
    41221 caattagcaa attgttgtaa aataggcaca atacttttac ctttacttgt taatgtatat
    41281 tcaacttttg gaggaatttc attatattgt ttacgatgaa caataccagt agactctaaa
    41341 tcttttaatg attgagctag catagtattt gtaatgccat ccaattttct ttttatgcta
    41401 ctatatctaa ttggattttc aactgctaac atacatataa taggtaattt ccacttacca
    41461 ccgacaatgt ctaatgcata acggattgga caggttaatt cttcaacgtt tttagttata
    41521 tttttttcag tatttatact cattttaatt actccttagt tatagtagta tcttttttat
    41581 actttatcag aaaaaactgc atacttgttt tttctgtatt ttatattaaa attatatcaa
    41641 aggataatcc ttaatacaag tatttaaaag gggtgtaaga attatgaaag cattagaatt
    41701 tttgaacaag gcacgagttt attatttagc aacagttgat gagaacaatc aagcacatgt
    41761 gcgtccttta ggatttgtta tggaatacaa tggtaaactt acattttgca catctaatca
    41821 aaaggatatg tttaagcaac ttatttttaa tccaaaggtt gaactttgtt gtatagacca
    41881 aaaccttaat actctacgta ttttaggtga agcagtattt gttacatcaa aagaaacaca
    41941 agctaaggca cttgaaataa tgccaagttt aggtcagatg tactctgtag gagatggtaa
    42001 gtttgaaata ttctctattg ataaggcaaa agttagttgt tgttcaatga gtggtaaaaa
    42061 gattgatgta gagctttaat tataattgaa tcgacatcca tagtgaaaat taagaaaacg
    42121 ttattactga aatgttgata gatttaattt aaatgcaata aaaagatttt taaataatga
    42181 atatgtaaaa atttttagtt attagatgtg tatttcaata atgatatact attttgaata
    42241 attacatact agaaataaga taattaaaat ttactttata atgggataga tttcttttaa
    42301 aaaatatttt aactataatt gatttttatt taatctgaaa aaaatattat gactataaaa
    42361 aattgctact tattatttgg atatttttat atat
//
LOCUS       DAELNY010000018        41178 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.018, whole genome shotgun sequence.
ACCESSION   DAELNY010000018 DAELNY010000000
VERSION     DAELNY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.018"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..1002
                     /locus_tag="KPE55_000807"
     CDS             76..1002
                     /locus_tag="KPE55_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBG0491904.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKNISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(1054..1944)
                     /locus_tag="KPE55_000808"
     CDS             complement(1054..1944)
                     /locus_tag="KPE55_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0491905.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            2132..3163
                     /locus_tag="KPE55_000809"
     CDS             2132..3163
                     /locus_tag="KPE55_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG0491906.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            3457..4332
                     /locus_tag="KPE55_000810"
     CDS             3457..4332
                     /locus_tag="KPE55_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBG0491907.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            4350..5840
                     /gene="cls"
                     /locus_tag="KPE55_000811"
     CDS             4350..5840
                     /gene="cls"
                     /locus_tag="KPE55_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0491908.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            6033..6317
                     /locus_tag="KPE55_000812"
     CDS             6033..6317
                     /locus_tag="KPE55_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG0491909.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            6350..7978
                     /gene="groL"
                     /locus_tag="KPE55_000813"
     CDS             6350..7978
                     /gene="groL"
                     /locus_tag="KPE55_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG0491910.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            8240..8980
                     /gene="larB"
                     /locus_tag="KPE55_000814"
     CDS             8240..8980
                     /gene="larB"
                     /locus_tag="KPE55_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG0491911.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            9064..9798
                     /gene="larC"
                     /locus_tag="KPE55_000815"
     CDS             9064..9798
                     /gene="larC"
                     /locus_tag="KPE55_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG0491912.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            9828..10247
                     /locus_tag="KPE55_000816"
     CDS             9828..10247
                     /locus_tag="KPE55_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG0491913.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKLSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      10307..10408
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10629..12164
                     /gene="guaA"
                     /locus_tag="KPE55_000817"
     CDS             10629..12164
                     /gene="guaA"
                     /locus_tag="KPE55_000817"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG0491914.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            12703..14133
                     /locus_tag="KPE55_000818"
     CDS             12703..14133
                     /locus_tag="KPE55_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0491915.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDID
                     NNTERYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(14281..14631)
                     /locus_tag="KPE55_000819"
                     /pseudo
     CDS             complement(14281..14631)
                     /locus_tag="KPE55_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14626..15147
                     /locus_tag="KPE55_000820"
     CDS             14626..15147
                     /locus_tag="KPE55_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0491916.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAFAATLGCVLCGVVIALFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(15276..16070)
                     /locus_tag="KPE55_000821"
     CDS             complement(15276..16070)
                     /locus_tag="KPE55_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0491917.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAEDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDRYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(16294..19722)
                     /gene="uvrA"
                     /locus_tag="KPE55_000822"
     CDS             complement(16294..19722)
                     /gene="uvrA"
                     /locus_tag="KPE55_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0491918.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTSPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            20041..22275
                     /locus_tag="KPE55_000823"
     CDS             20041..22275
                     /locus_tag="KPE55_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0491919.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFSSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            22531..24561
                     /locus_tag="KPE55_000824"
     CDS             22531..24561
                     /locus_tag="KPE55_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0491920.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            24558..25010
                     /locus_tag="KPE55_000825"
     CDS             24558..25010
                     /locus_tag="KPE55_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0491921.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            25012..26067
                     /locus_tag="KPE55_000826"
     CDS             25012..26067
                     /locus_tag="KPE55_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG0491922.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            26134..26442
                     /locus_tag="KPE55_000827"
     CDS             26134..26442
                     /locus_tag="KPE55_000827"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG0491923.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            26546..27811
                     /locus_tag="KPE55_000828"
     CDS             26546..27811
                     /locus_tag="KPE55_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG0491924.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGIFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            27899..28615
                     /locus_tag="KPE55_000829"
     CDS             27899..28615
                     /locus_tag="KPE55_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0491925.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGYEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(28810..29103)
                     /locus_tag="KPE55_000830"
     CDS             complement(28810..29103)
                     /locus_tag="KPE55_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG0491926.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(29109..29324)
                     /locus_tag="KPE55_000831"
     CDS             complement(29109..29324)
                     /locus_tag="KPE55_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491927.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            29772..30170
                     /gene="tnpA"
                     /locus_tag="KPE55_000832"
     CDS             29772..30170
                     /gene="tnpA"
                     /locus_tag="KPE55_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0491928.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            30167..31330
                     /locus_tag="KPE55_000833"
     CDS             30167..31330
                     /locus_tag="KPE55_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0491929.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            31690..32268
                     /locus_tag="KPE55_000834"
     CDS             31690..32268
                     /locus_tag="KPE55_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0491930.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            32307..32885
                     /locus_tag="KPE55_000835"
     CDS             32307..32885
                     /locus_tag="KPE55_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0491931.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            32971..33600
                     /locus_tag="KPE55_000836"
     CDS             32971..33600
                     /locus_tag="KPE55_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0491932.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            33590..36349
                     /locus_tag="KPE55_000837"
     CDS             33590..36349
                     /locus_tag="KPE55_000837"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0491933.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            36436..37998
                     /locus_tag="KPE55_000838"
     CDS             36436..37998
                     /locus_tag="KPE55_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG0491934.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            37999..39135
                     /locus_tag="KPE55_000839"
     CDS             37999..39135
                     /locus_tag="KPE55_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0491935.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            39199..40275
                     /locus_tag="KPE55_000840"
     CDS             39199..40275
                     /locus_tag="KPE55_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG0491936.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            40253..>41178
                     /locus_tag="KPE55_000841"
     CDS             40253..>41178
                     /locus_tag="KPE55_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HBG0491937.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGT"
BASE COUNT        15852 a         4488 c         7254 g        13584 t
ORIGIN      
        1 atttacaaaa taagagatag tagcagtaca atataagtat aaggtaaaag tattatatat
       61 tagggagggg taagtatgaa aaatcaatta aatgaaaatc ataaaaaggt tgtatttttt
      121 ggagtaggtg ctgtaggagc tacttttgca gaacaatttt ttaactctaa atatgatttt
      181 aaaattcttt gtgataatga aagaaagaaa agatatttag aagaaggatt tataataaat
      241 ggaaaaaggt atgattttga ttatgtaact aaagatgagt ataaacaaga ggctgatttt
      301 ataattatag gtctaaaata taataattta aaagaaaata taaaagaatt agatggatta
      361 gttggaaaaa atacagttat aatgtctctg ctaaatggag ttgatagcga agagataata
      421 ggagaaagat ttggaattga aaaaatggta tattcatatg ttaccaatat agatgcaaag
      481 aaaatcaata ataatattat acacactact aatgggataa ttgtatttgg taacaaagat
      541 aatagtgaag atagaaaaac taatataata accgaagtct ttgatgatgt aaatatagaa
      601 tatactttat caaaagatat tcaacgagat atgtggtgga agtacatggt taatattggt
      661 gtaaatcaaa cttcagctat acttggtgca ccttatggag tttttcagag ttctgagcat
      721 ttaagagaat tagcaaaatc tgcaatgagg gaagttgttg ctatagcaca agcaaaaaac
      781 atatctctta cagaagatga tgtggaacat tcattacata gaatactaga acattcaaaa
      841 gaaggaagaa catcaatgct tcaagatgtg gaagctcata gacttacaga agtagatatg
      901 ttttctaaga atatctgcaa acttggaaaa aaatataaca tacctactcc tataaatcag
      961 actttctttt atatgataaa agtaattgaa agtagatttt aaagggtctt tgtaagaaat
     1021 attgataatt attgatttta aattttatta atgttatata ctatttaagt tactaaatat
     1081 ttttaactct tcaatgaaaa tagttgctgc ttttgataaa gttatatctt ttggaaatat
     1141 atatgagaaa tgtctgttgt agctgcttcc aagctctatg acagacagct cattgtttag
     1201 aacaggtaag cttgtcacta gattagagac tattgtaatt cctaaattat ttctaacaga
     1261 ttctttgaca gcataattac ttccaaaaac catcatactt tttggaataa tttctttaac
     1321 acttaaaaac atatctaaat aatctctggt tccagaacca tcctccctga ctatccattt
     1381 ttggttttga agtttatcaa aactaaaatc ttttaataaa tgactcttgt atggaagtgc
     1441 taaaaccatc ttatcttcaa aaaaatattc ttgaataaat gaaggggaag agcaagtgcc
     1501 ttcaataaga ccaatgtcta aaatataatc cttaacatga gaagatacga ttgatgtatt
     1561 ttttataaaa acttctacat ctatgtcagg atacttttta gaaaataatg ctagaaaatt
     1621 tggtaaaata tactctccaa tagttaaact tgcaccaatc tttaagtgac cagttatagc
     1681 atctgaaact tggcttacat catgataagt aatatttaaa agatttaata tttctttagc
     1741 tcttttgtac aagatttgtc cactttctgt tataaaaatg gttttttgtt taatggacct
     1801 gtttataaga acaactttaa aataattttc tagatttttt atgtgtttac ttacacttgg
     1861 ttgtgataga tttaaatatt ccccagcttt agtaaagttt ttgtattcaa caacagctac
     1921 aaatgttttt aattcttcaa acaatttttt gtctccttta cctaatgaaa ttttacattt
     1981 tttatatact ttctacagat aattatacta tatataataa ccattaaaaa cattaatcat
     2041 tacgatatta tatatttatt ttacttatgg ttattacaat tatatactat tgctatgaag
     2101 aaaactatat taattataag gtaggtatat tatgttaaag aaaaaaagca gtttgacatt
     2161 aaaaactata agggaaatat taccagggct gtttgtatca gtattagtag gttatattag
     2221 tatatttatt tctattttaa ttcctaaggt aggggcagca tcaatttcta tatttttagg
     2281 tatgtttgta ggaaacctat ttttaaatca aaaggtattt caaaaaggtt ataaattttc
     2341 agagacagat ttgttatcat attcaatagt actattagga gcaacactca gtgtatctac
     2401 cttaatagac ttaaaagttt caggaatttt atttataata ttgcaaatga caataacaat
     2461 aatagctgca ctttatatag gtaaaaaact aggatttgaa gaaaacttta gatttctaat
     2521 ggcaagtgga aatgcagtat gtggttcttc tgcaatagga gcaacagttc cagtggttaa
     2581 tgcgacagat aaagaaaagg gaattgcagt aactattgta aatgttactg gtatatttct
     2641 tatgttttta cttccaataa tatctcaatt tttatatagc catgaacttg taaaaacttc
     2701 agcaatgata ggtggaactt tgcaatcaat tggtcaggtt gtagcaagtg gagcaattgt
     2761 tggagaaaat gtaaaagatt tagctactat ttttaagata gtaagagtaa tatttctagt
     2821 tgttgttgta ttagtatttg gacatatgaa aaatcaatct aatagggaaa ttcttgaaga
     2881 agaaaaggat gagataaaaa agaaaaaagt gactatacca tggtatgtaa ttggattctt
     2941 tataacttgt gcactttttt caatgaacat tataccacaa gaagtatctg tgttgtgcaa
     3001 agaaataagt aacaaactag aaataatagc tttagcagca attggattaa aggttaatgt
     3061 aaaagattta gtgaaacaag ggaaagaagt ttctttatat ggtttatttg taggtacagt
     3121 acaagttgta tcagctgtag ttttaataaa aatatttata tagcataaaa atagtatttg
     3181 taatattaat ttgattaatt aaaataatat gatactaata atttataata taaataaaaa
     3241 atgaatttct ataagtttaa gtgggtataa acttatagaa attcattttt tatttaaggt
     3301 aaaaatttaa agtaaaatat tattttacat aataataacc ttataaattt tgaaattact
     3361 tatctttgtg ttaaaataag taaaatgcac atattagagc taagttttta agtaaataaa
     3421 caaagtgaaa atataaatat taaggagatt tggtatatga atgtttatga aaaaggtaat
     3481 ggagttatac tagaaggagt aacagatttt gacccagtac atatatttga gtgtggtcaa
     3541 tgttttagat ggcacaaaca agaagatggc tcatacactg gagttgcaaa ggggagaata
     3601 ttaaatgtca aaaaagagaa tgataaaata tatttaaata atactaattt aaaggagttt
     3661 aacagcattt ggtataatta ctttgattta ggaactgatt atacagaaat aaagaataag
     3721 ctaaaaaata tggatgaata tttaaataag gctactgaat ttggatgggg aataagaata
     3781 ttaagacaag atggatggga aatgcttata tcatttataa tttcctctaa taataggatt
     3841 cctatgatac aaagagctat agaaaattta tcaagaaagt ttggtaagta tataggtgaa
     3901 tatgaaggca atgaatacta tgcattccca acaccagaag aactaaataa ggcctcacaa
     3961 gaggaaataa gagcatgtca aacaggtttt agagataagt acataaaaag tactacacag
     4021 gcagttatag agaataatga tgaggtatct gaatatacaa atttaagtac agaggattgt
     4081 agaaaagagc ttttaaaatt taatggagta ggaccaaaag tatgtgattg tattgcatta
     4141 tttggtatgc aaaaatatga ttcttttcca gttgatgttt gggttaagag agttatgcaa
     4201 gaattttata tagatgaaga catgagttta cctaaaatga gaacctatgg aatcgataaa
     4261 tttaaagaaa tgtctggatt tgctcaacag tacttatttt actatgcaag agaactcggt
     4321 ataggaaaat aaattaaggg ggagaaatta tgggagtaat aggaacaata tttctgttct
     4381 atttaattat atcatatcta gctggagcca taatttctgt gattatatta cttgaaaata
     4441 gagaccctgc aaaaacaatg tcttggctat tgatgtttat tatatttcca ggtgtaggtt
     4501 taatgatata tgctatttca ggtagaaata ttagaaaaag aaaattattt aagactcaaa
     4561 aactagctaa taatataaaa gaaaagaaac tatttgatac attagaaaag ataacagaaa
     4621 tagttgaatt agaaaaagaa tctataaaac aaaataagct tttaagagat gaagaggatg
     4681 gtagttatag aaaaagggta ataaatatgt tgttaaagac tggtatgttt ccctttacta
     4741 aaaataataa agtggatgta tttgtagatg gaaatgaaaa gtttaaaaga cttatagaag
     4801 atataagaga agctaaagac catattcatt tagagtactt tatcatcaaa gatagtgaaa
     4861 taggtagggt gttaaaagaa gaacttataa aaaaggctaa agaaggcatt aagataagaa
     4921 ttttatatga tgatgtagga tgctggagat tttggtttaa tagaaaattt ttccgagaaa
     4981 tgagggaagt aggtatagaa attgcagcat ttttacctac aaaatttcct ataataggag
     5041 ggaaattaaa ctatagaaat cacagaaaaa ttgttgttat tgatggaata attggatata
     5101 caggtggaat aaatatagga gatgaatatt taggcaaaaa tgataagttt ggatattgga
     5161 gagatactca tattaggatt aagggtattt ctgtatacat gcttcaaatg acatttctga
     5221 ttgattggta ttatacgaca aaagaagtat tagtgactaa aaattatttc ccaagtgtag
     5281 ggaatgttgg tgaaagtatg atacaagttg ttgcgagtgg tccagacagt gactgggaag
     5341 atattcatta tgcttatttt tctgcaatat gtcaggctag aaaaaatgtt tatatagaaa
     5401 ctccatattt tattccagat gaaagtctac taaaagctat aaagagtgct gctcttagtg
     5461 gagttgatgt gagaataata tttcctaaaa ttgcagacca caagatagta aatatagcat
     5521 cttattcata ttttgaagaa atattaagag caggtggtaa agtttattta tataataaag
     5581 gatttataca ttctaaagtc gtaataatag atgataaaat tgcttctgca ggtacagcta
     5641 atatggattt aagaagtttt atgcttaact ttgaggtaaa tgcatttatc tatgatgaag
     5701 aagttattag ggtaatgaca gatgatttct ttgaggattt aagttactgt gaagaactta
     5761 atttggaggt atttaagaat agaaatatca tacaaaaaat caaagaatct gtagcaagat
     5821 tattttcacc aatattataa aaaattatta aaaaaggtat tgaaaattat gtgtgattgg
     5881 tatattataa taattgttag cactctatta gatagagtga taataaataa aaagtatata
     5941 tataaatcta catatataag tagtattaat ttaaaaataa ctaaaaacat aggcgaacta
     6001 gtctatactt aatattttag gaggcgtgaa taatgaaaat aagaccatta gctgacagag
     6061 tagtaattaa aaaagtagaa gcagaagaaa aaactgcaag tggaatagtt ttaccaggag
     6121 cagctaaaga gcaacctcaa atagctgaag ttgtagaagt tggaccaggt ggaatagttg
     6181 aagggaaaga aataaaaatg gaattgacag tgggagataa ggttatattc caaaaatatt
     6241 ctggaacaga agttaagata gaaggacaag aatacacaat actaagacag agtgatgtat
     6301 tagctgttat tgaataaata tagaataaat ttattaggag gggtttaaaa tggctaaaga
     6361 aattaaattt tcagaggaaa caagaagagc tttagaagct ggtgtaaata aattagcaga
     6421 tacagtaaaa gtaacattag gacctaaagg aagaaatgtt atattagata aaaaatttgg
     6481 ttctccactt ataactaatg atggagtaac tatagcaaaa gagatagaac tagaagatag
     6541 atttgaaaat atgggtgctc aattagttaa agaagtagca actaaaacta atgatgtagc
     6601 tggagatggt actacaactg ctacagtttt agcacaagct ataataagag aaggtttaaa
     6661 gaatgtaaca gcaggggcta acccaatact tttaagaaaa ggaatacaaa aagcagtaac
     6721 agtagcagta gaagaattaa aaaatcaatc aagaatagta gaaacacaag aggctatatc
     6781 tcaagttgct tctatatctg ctggtgatga agaagttgga aaattaatag cagaagctat
     6841 ggaaatagta ggtaaagatg gagttataac tgttgaagag tctcaaacta tgaatactga
     6901 attagatgct gttgaaggta tgcagtttga tagaggattt gtttctgcat atatggttac
     6961 agatgtagat aaaatggaag cagttttaaa tgacccatat atattaatta ctgataaaaa
     7021 aatatctaac atacaagaat tattaccagt tcttgaacaa atagttcaac aaggtaaaaa
     7081 gttattgata atagctgaag atgtagaagg tgaagcatta tctacattag tagtaaataa
     7141 attaagagga acatttgatg tagtagcagt taaagctcca ggctttggag atagaagaaa
     7201 agaaatgctt caagatatag caatacttac aggtgctcaa gtaatatcag aagaattagg
     7261 ttatgattta aaagaagctg atttatctat gctaggtaga gcttcatctg ttaaggtaac
     7321 taaagaaagt actacaatag tagatggttc tggagataaa aaagctatag aagatagagt
     7381 aactcaaatt aaacatcaag tagagcaaac tacttcagat tttgatagag agaaattaat
     7441 ggaaagatta gctaaacttg ctggaggagt agctgttgta aaagttggag ctgctacaga
     7501 agtagagtta aaagaaagaa agttaagaat agaagatgct cttaatgcaa ctagagcagc
     7561 tgtagaagaa ggaatagtag ctggtggagg aactgctttt gttagtgtta taccagcaat
     7621 aggaacacta attgagagtt tagaaggaga agttaaacta ggtgctcaaa tagttaaaaa
     7681 agcgttagaa gagccattaa gacaaatagc tataaatgct ggtcttgaag gtgctgtaat
     7741 tgtgcaaaat gttgttaatt ctgaagcaga aactggattt gacgcattga atgaaaaata
     7801 tgtaaatatg atagaagctg gtatagttga cccaactaaa gttagtagaa gtgctttaca
     7861 aaatgctgca tcaatagcaa gtactttctt aactactgaa gctgctgttg ctgaccttcc
     7921 tgaaaaagaa gatgcaggaa tgccaggaat gggtggcgga atgcctggta tgatgtaatt
     7981 taaaaaattt aaaatataaa ttttaatata aagaactatt tcgacttaga ttttaaactc
     8041 taagttgaaa tagttctttt gtatatatct aatttttgta tatctataaa ttcaatacac
     8101 atatttagat aatcagtatc tagtttgtaa taatatttta gagtagtatt gttatttttt
     8161 aggttatgat tttagatata tattaattaa ggtataattg ttgatagaga ataaatcaaa
     8221 tttaaaagga tgcgataata tggacttaag aaaattgctt gaagaagtta aaaatgaaaa
     8281 tattgatata gattttgcgt tagaaaagtt aaaagattta ccttatgaag accttggata
     8341 tgcaaatata gatcatcata gagaactaag aaatgggtat ccggaagtta tttattgtga
     8401 aggtaagagt gatgagcata ttttagggat tattgataaa atgaatcaaa aaagttctaa
     8461 catattggga acaaggtgca gaaaagaaac ttttctgaaa attaaagaaa tctataatca
     8521 tgctgagtat gaagaggcgt ctaaaattct taagattcaa aatcatgata tagagaatat
     8581 aggaaaaggt aaaattgtga ttgctacagg gggaacttct gatataccag tagcagacga
     8641 agcttatcat acagctaagt ttttaggtaa tgatgttgat agaatatatg atgttggagt
     8701 ggcaggtata cataggcttt taaataaaag acataaaata gatagtgcta gagtaatagt
     8761 agccgttgca ggtatggaag gtgctcttgc aagtgtaatt ggtggactgg ttgatgttcc
     8821 agtaatagct gttccaactt ctgttggata tggagcaaat tttggaggat tagcagcatt
     8881 actagctatg ttaaatagct gtgcatctgg aatatctgtt gtaaatattg ataatggatt
     8941 tggagcagga tatttagcat caatgataaa taaactgtaa atatattgat ataaattaaa
     9001 caaaactata taataaaatt taatttctat tatattttta ctattaaaat tgaggagaca
     9061 tatatgagtg aaaaaatact ttattttgat ataataagcg gtatatcagg ggatatgacc
     9121 cttgcaagtt tattaaattt aggtgtgcct aaggaaattt ttttggagga aattcataaa
     9181 ctaagaatga gcgatgaatt taatataaac atcagctcga aaactgaaaa tggaatagta
     9241 ggtacaaaag tagaagtaat tacaaaagaa aaacatacac atagaaattt agtggatata
     9301 tttgaggtta tagataaaag cactctaaac gaaaatgtaa aatctaaagc aaaaaaaata
     9361 tttatggtaa ttggagaagc agaagcaaaa gttcatggaa ctacaataga caagatacat
     9421 tttcatgagg taggggctat agattcaatt gtagatatag taggagcttc tatattagtc
     9481 gacttacttt gtgtagataa agtttgtgct agtacagtgt ctgttggttc aggatttgta
     9541 aaatgtgaac atggaattat acctatacct gcacctgcaa ctatagaaat tttaaaaggt
     9601 gtgccaatta aattaaataa tgtaaatgga gaatgtacaa ctccaacagg agctgcaatc
     9661 ataaaagttt tgtgtgatga gtttgtagat gagcttgaat tttctccaaa gcaaattgga
     9721 tatggtatag gtcataagaa gtttgaagta ccaaatatac tcagaactgt attaggtgaa
     9781 aaaaaaaaga agaattaatt tatgaaatta gtgcaaacat tgatgatatg tcttcagaaa
     9841 tctattctta tttgtatgaa aaaatattag atgagaaagc attagatatt tatacagaga
     9901 gcatttatat gaagaagaat agaccagcaa caaaactatc aattttatgt aatgaatcag
     9961 atttagaaaa atttattaaa atattacttt tagaaactag tacttttgga gtgagatata
    10021 atgcatataa tagagaaatt ctagagagaa aatttgtaaa attagataca aaatatggag
    10081 tggttacagt aaaactaggg tattataaag gaaaactagt caaggctact ccagaatata
    10141 atgaatgtaa aactatagca aaaaatgaag atattcctat aaaaaaagtg tttgctgata
    10201 taaattttag aataaaagaa gaatttagtg aaaacttatt gacataagta ataaattttg
    10261 ataaattaat attcagcctt attaaataaa agtaaacaga aaaaaatata taaaatataa
    10321 actcactcgt atatgctcag aaatatggtc tgagagtctc taccaagata ccgtaaatat
    10381 cttgactatg agtgaaatta ttataccaaa agacctagtt aagtaaatgt gtaggctgag
    10441 ttaaactccc tatgtgttgg ctataagata aaggtatttt ggaagcctgt atagtaattt
    10501 cacttttgct agaaattatg gaagttacta tacaggtttt ttgtgtaaaa agatttaata
    10561 taatttagac tgtattgtgt taaaataaat atataaaaac tatattatac aaggagagat
    10621 gtaaatttat gaaacatgaa ttagtacttg taatagattt tggaggacaa tataatcagt
    10681 tgatagctag aagagttaga gaaaacaacg tatattgtga aatacttcca tgtactgcaa
    10741 gcatagaaag aataaaagaa aaaaatccta aagggataat atttacaggc ggtccaaata
    10801 gtgcgtattt agaagattct ccaacaatat caaaagaaat atttgaacta ggagtaccaa
    10861 tacttggaat atgctatggt attcaaataa tgtctcatgt attgggtgga gttgtaagaa
    10921 aaggtaataa acaagaaaaa gaatatggaa aaacagctat aacttatgga aaatcatcat
    10981 tatttgaagg aataactaca aatagtgtat ggatgagtca tacagattta atagaaaaag
    11041 ttccagaagg atttactata gttgcaaata ctaatgattg tccagtagca gctatggaaa
    11101 atgtagaacg aaacctatat ggagttcaat tccatccaga ggtagaacac tgtttggaag
    11161 gggacaagat acttacaaac ttcttatata atatttgtaa agtaaaaggt gactggacta
    11221 cagattcatt catagaagat aaaataaaag aattaaaaga aaaaataggc gataaaaaag
    11281 ctttatgtgc tttaagtgga ggagttgatt cttctgttgc tgcagttctt attcataaag
    11341 caattgggga caacttaact tgtatatttg ttgaccatgg tttacttaga aaaaatgaag
    11401 gaaatgatgt agaaagaata tttagagaga aatttgatat aaatttaata agagttaatg
    11461 cagaagatag attcttatct aaattaaaag gagtttctga accagaagct aagagaaaaa
    11521 taataggtga agaatttata agagtatttg aagaagaatc aaataaatta ggaaaaatgg
    11581 atttcttagt acaaggaaca atctatccag atgttataga aagtggacat ggaaatgctg
    11641 ctactataaa gtctcatcat aatgttggtg gaatcccaga agatgtagac ttccaagaaa
    11701 ttgtagaacc attaagagag ttatttaagg atgaagttag aaaaatagga ttagagcttg
    11761 gtatagaaga aggattaata tttagacatc ctttcccagg accaggtcta ggaataagag
    11821 ttataggtga tgtaactaaa gaaaaatgtg atatattaag agaagctgat gctgtttata
    11881 tggacgagct tagaaaagca ggtttatata gagaaatatg gcaagctttt gcaacattac
    11941 cagatgttaa gactgttgga gttatgggtg atgaaagaac atatgcttat ttagttggtt
    12001 taagagcagt tacttcttct gatggaatga cttctgactg gtataagatg ccttatgatg
    12061 tattagaaag aatttctaat agaataataa atgaagttga tggagtaaat agagtagttt
    12121 atgatataac ttctaagcct cctggaacta ttgaatggga ataaaatata gaatgccaaa
    12181 aatccacata aatacaggat ttaaggtctc tcttacttct atgttatact ggtttgatac
    12241 tatcaatatt tgccagtatc ttatggatta ataagcaaaa aacatcctgc tgaatgataa
    12301 attcagcggg gtgtttttat tttgttttag aactcctttg ctctgaaact cgaagtattt
    12361 aacactgatg attttgttat cttttagttc cttttgtgtc atgctctgaa agacaaattg
    12421 atacaataaa aaagtaattt cttaatcatt gatggagata tgtatttatg ttactcttga
    12481 tggtaatttg agtaatattg ttcatagctt tttatattca cttaattaga gaatcatttg
    12541 gtataatcat aaataaactt gatgatataa tagaaaagat ttataattat tagaagtata
    12601 taaaagtatt ttatgtctat gaaatgtatg tctataaaac atatagatga cttttgtata
    12661 ttaatgtatg aatattgata ttatagaaga attgagttgt caatgatact aaatcaaata
    12721 tttatagaag ctggagggat tatgagaaat aaaataatta taggaattac tttttcactt
    12781 gtattactaa tgtctggatg tgcttcaaaa caaacagcag aaataaacgt attagcaact
    12841 actgatttac atggagttat accttatgag ttaacttctt atgtaaaaga agaaagaaaa
    12901 aaagataaaa atctaacttt agtagatgca ggagattttt ttgatagtgg agaagtgttt
    12961 ggttctagca tggatagtta ttttagtgct aggtataagg atattgataa taacactgag
    13021 cgttacattg agacacctat ttcaaaagat atgaaagagg taggttatga tgttgcagtt
    13081 cttgggaatc atgaatttat atccaacaat aaattttatc tagataatat gatatctgat
    13141 tttgagaagc aaaaaataaa tattttatca gcaaatacgt ataaaaaaaa tggagacagt
    13201 tatacaaaac catacataat aaaaaatatt aatacacctg aaggaaacgt aaaactagga
    13261 attttagggc ttactataaa agaagttggt gagaggaaac agtggaaaga tggaaaactt
    13321 gttgactcta agtctttaga actaaaagac caagagggct acaatggtga attgtatatg
    13381 aatgatttag tagaagatgc taaaaagtgg gttagtataa tggaaaaaga gaagcctgac
    13441 attatagttg ctgtggcaca tacaggtgag aaacctaaga aacctagaaa cccaggaaat
    13501 agaatacaag acttagctca acaagttgat ggaattgatg ctatagttgc aggtcataat
    13561 catgttcaaa ttgagcaaca tgattataaa aataaatcag gagaaaatgt tatagttact
    13621 gagccaggaa agcatggaga atgtatttct aagattaatt ttaaattaga aaaaaacaat
    13681 gataaatgga atatagttga caaatctagt aaattaatta aatttgaaaa aaatcaagaa
    13741 agtgataaat ttggagaatt tatttcttca attaataaag taagtaaaga taagaaagaa
    13801 ataagattgt cagaagtaaa gactcctgaa tgggataaaa tatatgtttt taaatctgga
    13861 acatctgcag atgatatata taaaacaata ggatataaat ggagaaatat atcatctcca
    13921 aagggtgatg acatggtgca gattgttgta atgaataata ataagcctgt tagctatgta
    13981 tatggtgaag ctgaagttat gccaattaat ataaaatgtg atgaatcaga gtataaagat
    14041 aatgttgtaa ctatttatcc taataaaaat gataaattta aaattgaaaa aggtaaacaa
    14101 gaatatagta ttaatttaaa acatatacaa tagaaacttt tatcttattt aagtttagat
    14161 tgtacttaaa ataaagtgtg tattatttta aaaattgatg ataattaaaa tcacaacatt
    14221 aaggaatagt acacactttt tatgctaaaa aatagaatgt taaaaaatta aacctttaaa
    14281 ttaaagtaca ttgcttatat tatccaatat tatcttcaaa ggttgtgtga tatttttatc
    14341 cttatgtaat attgctaaca ttttattttc catatctatc ccatctaatt caatcaatcg
    14401 caatttttta tgtaagagtg aatctaataa taaattttct ggcaaaatgg ttatacctaa
    14461 ccctgcttct gcagctttaa taagtgcaaa agaattaaca ctttcccaaa caggataagc
    14521 tttttggttt gaaagagata atacactgtc caaagtattg cgaatggcac ttccttgctc
    14581 acgtaacaat aacggaaacg tgcataactg tcgtagagat aaggcttgtt tagtattggt
    14641 ggtggttatg caatcatacc tttaatccaa gagaaagtag taaatcaaac tggatggata
    14701 agtgagaaaa tgtttacaga tattataact atttcacaaa tgacaccagg gccactggct
    14761 gttaatactt ctacttttgt tggacttcaa attggagggt tttggggagc atttgcagcc
    14821 actttaggtt gtgtattatg tggtgtagta atagctctat ttttatataa tttttttcaa
    14881 aagcatcaga actctactta tatatttgaa atattaaatg gtctaaaatc agcttcatta
    14941 ggtttaatta tctcagctgc ggctacaatt ataattcttg cattatttgg gacgaataaa
    15001 gtgtacttca attctatatt tgagatgcta aactggactg cactaataat cttttttgtt
    15061 acactactga tagtacgtaa gtggaaaatt aatcctattg tgattataat aataagtggt
    15121 atactgggaa tattttttta tttataagtt tttgctaagt aaaggatatc tattttttta
    15181 tatcttaaat ccaaatagcc aataaagtaa ggctatttgg atttttaata aattctgaaa
    15241 aaataatcag aaacccatat aacatttaaa ttatatcact ggttttctac aaaccaatca
    15301 taagcttctt ggcaaaattt ctcactatct atatagtaac tattatgaac attattaatt
    15361 tcaacaagtt tctttttaca ttttagtgaa ttataatatc tatcaaaata ttgggtttta
    15421 aaatatctat catgaagtgt cttaaagaca agagtaggta tgttgactcc atcttttatc
    15481 ttactatctt ctgcaataaa gcattctaac attgtattaa ttatctttgt actatttttc
    15541 caaatatgag gatttttctt ttcaaatatt ttataaaatt cattatcata gacttcatta
    15601 taacctttat atttaggtgg ttttactgtt atgtgaaaat taggaagaaa cttaaagaaa
    15661 aagtttaata atttaacagt gagataggta tagcctactc ctgttgtgtt tttataatca
    15721 acacaagcaa actgagcaaa actttttaac ttattgtctg aacatgcata atattgtgca
    15781 aacataccac caattcccgt attcccatat agatgtatgt tggaagaata atttttttct
    15841 atgtatgtaa tgacagaatc taagtctttt acaattgaat tgcgagaaaa atctttttca
    15901 tatccatcac ttttccctgt tcctgaaaaa tcaaaagcaa atatatttac attatgtttg
    15961 gcaagtggtt catatgcttt ttgcatctca agcgtagtga caataggagt atgtgtaaca
    16021 acaatattca tggatgattt tctggtaata aatgagttac aaaaaatcat tgtatctcca
    16081 tttggtattt ttatatgttt ccaactcatt tgattttcct ttctctatta taaaatataa
    16141 taatttttat gttctgtttt atatattatt catattttat cacagataat atactaaagt
    16201 taattttttt attgtacacc tattataata attcttctat aatataagac attctataat
    16261 tttatattta agttcatgtg tatttaattg tttttatata aaatgtccag taatactatt
    16321 tggatttatt tttatttcat caggagttcc agaagcaaca atttcccctc catttattcc
    16381 actgtctgga cctatatcga taatccaatc agaagcacgt ataacctctg tattgtgttc
    16441 aataattaaa agtgaatgct gttcatttac aagttttcta aaagtaatta acaaattttc
    16501 tatatcttta aaatgaagtc cagtagtagg ctcatccaat atgtacagca ttcgtttttt
    16561 agtttttgtc cctaattctt ttgctaattt tatcctttga gattctcctc cacttaatcc
    16621 taatgtattt tgtccaagct ttagatatcc aagtcccact tcatctaata aggagagttt
    16681 tctatatata tcagagcaat cgctaaaaaa tgtttttgct tctgctactt ccatgtcaag
    16741 tatatctgca attgtctttc ccttgtattg tatagacagt atttcttttt gatatctctt
    16801 accttgacat tcactacaag ttacccattg gtctgccata tattgcagaa taatttttgt
    16861 tttacctaat ccttgacagt ttgggcattg cccctttttg ctattaaagc taaaatagct
    16921 ttcatccaat aatttttgtt ttgcttgtgg agtttcagca aatagagaac gaatttcatc
    16981 aaaaatattg atatatgtag caggtgtaga acggctagat tttccaatag gtgtttggtc
    17041 cattaagata aaatcatcaa tagcatcaaa tccagtaaat tcagtataat ttttatctgg
    17101 gatagatttt tgtttcaatt tttcttctag agcaggaagt aaactgtgaa atacaagaga
    17161 gctcttacca gaaccactaa cacctgtaat agagcacata cagtttaagg gaatttctac
    17221 atcaatattt tttaagttat ttgcatggca tccttttaag cttaaccatt tacttggtat
    17281 agttttattt tgtgaacctg ttttattgta tgtacttaaa tatttacctg ttatagagtt
    17341 tggatttttt ataatttctt gggttgtacc ttctgcaata atgaaccccc cttttgtacc
    17401 agcacttggg cctacatcaa taatataatc agcagacagt attgtatctt tatcgtgttc
    17461 gacaactata acagtattac ccttatctct tagctcttct atcattttga tgattaaatt
    17521 atggtctctt gggtgtaaac caatagatgg ttcatctaaa atgtaggtta agcctactaa
    17581 ttcacttcct agctgacttg ataatcgtac tctctgcagt tctcctccag ataaagttgg
    17641 agatgttcta tctgttgata tatagctaag acctaccttt aatagataac taactcgaat
    17701 ttttaattct gttaagatat catttaccaa agagagctca tttttttgaa gttgatttgg
    17761 taattcacac aaccaattcc ataactgttc tattgtcatt tttgttagtt cagggaagcg
    17821 ataacctttg attgtagtga atctagcctc aatgcacagc aattcgccac cacatgtatt
    17881 gcaaggaatt ttgttcatat attgatgcaa ggtattattt tcagaactag attctctaaa
    17941 aagtctttga atgtggttga ttgctcctga agcaggtctt ctaatctttg attctcgtcc
    18001 ctttgattca aagctaaatt catatatctt atcgtctgta ccatataaga ttgcatctat
    18061 aaaatctctt ggtaattcat tccaaggtat atctaggtct atatttttat cttttgctat
    18121 tgcatataat tcacctacca tccaattacc atttggcttt ttccccctca attttccaaa
    18181 atagtaagtg gctccatcta atattgaaat agttgggtct tttacaatca attgaggatt
    18241 aacttgatag gtaaaaccta atccattaca atcgtgacaa gctccagtat gggagttagc
    18301 attgaaggtt gaagtattta tcttagaaaa aatagtttga caatgcggac aataattttt
    18361 tgtattattc tttttttcta atggaatttt acaatatgga cagtaacttt ttccgattga
    18421 tacaaataac attcttaaat aatcccaaat gcttgtaaga gtaccaacag tagagcgagg
    18481 attttggctt ccttttttct gttcgatagc tatagtaggg gttaatccct gaagtaaatc
    18541 aaattccggt ttttgaagct gttctgaaag ttttgctcca ttacttagat tttcaaaata
    18601 acgtcttttt ccttcttcat aaatcgtatc gaaagctaaa gaagattttc cactaccact
    18661 tacacctgta ataacagtta tttgattttt tggaattgta acctcaacat cctttaaatt
    18721 gttttgcctt cctcctttta aatagataga gcttttatta tgacaaagtg aatttttaat
    18781 tgatttattt attgattttg taaatttgaa tttcttttct agttcaagag gattgcctaa
    18841 ttcttttatt tccgaaatac ctaatatttt taactgagaa aagattgatt tcaaataatc
    18901 ttcaggcaat ttttctatta acaaattaag gtcttctatt tttatatttc cgcttgataa
    18961 tatttctgcc atttcatcta ataaattttt ttctgctctt gttaattgat tgtatttttt
    19021 ggaacttaat tctgtccata aatcccatgg aagcaattct aaaccaatca tattattaaa
    19081 gtcatgtaga agattaccct ttacagaatg ccaacctttc caatttttat tatatccaaa
    19141 gtcatctgga tgagcatgtc cttgccgaca tcttatccag gcttctgaac cagttaaaaa
    19201 accatctgtt tttccgacat catgagtgtc aaaattaatt tgaaaataat ttttttgtat
    19261 gtcatctatt tgtgcatcta ttcttatcca tctctttgtt agagtatcat agtattctac
    19321 aagccagtgg tcttcataag taagctcact ttcaaaataa ttagcaaaac ctgctctcat
    19381 tctagcctca aaacctcgat atcttaggag cgatacaaga agtaaagaat aatctctgca
    19441 cattccaaca agcctttgtt ttgggtttct tggaatagtt agtgggttag aatttatttc
    19501 aaatattctt tttaacattt gaggaattgt acgaagcaat tcctcatcag attgttgctt
    19561 attaaaggat ataccatata attttccttg gtctccatga attactaggc tttgaaccac
    19621 ttttaataac tcaattggag tctttggtaa atcttgatat ttattttcaa attttttagg
    19681 atttgtgatg atgctttgat ttttatagtt atttattttc atttatattt cacctctcta
    19741 aatggatgat ataccctgac atggtgtcat atgcaaggac aatttaaatt ttttgtccaa
    19801 aatttaaaaa taaataaaaa atgatacagt gataaattgt aatacaaata tagtttcaaa
    19861 ttgcatataa tataattatt taataaaacc attatctatg gaatgttaat gaaatgttaa
    19921 gatggcattc ttgaatatta agaaatagaa tactataata tagtgtgaag gattcttagt
    19981 aaaaaaattt gtaagtgtta atatacatta aaaaataggt ttataatttg gaggaaaaaa
    20041 atgaaattat caaggtataa gaaaaatagt ttagttctgt ctataatttt atgttcactt
    20101 attatttcaa tccttgtaat tactgttttt aataccgtaa aactacacaa tatatctaat
    20161 aaacaatcta aaaactattt aaatgatgta tctacacaga ttgtaatgaa tgttgattct
    20221 aaaatcaaat ttattttatc cgacttaaga ataatggcag attttattaa gcaatatgaa
    20281 ggtgatagtc gatatgagta tttatctaaa agaaagacta gttataagta ttatgatgtt
    20341 ggaataatag atttagaagg taaagctgag tttttaagtg gaaaaaagtt tgacttaaag
    20401 aatactgctt cctataaaaa aggaattgaa ggtaaagaat actcagaagt aatagagtca
    20461 atgggttttg ttctttatag tgtacccata tttaatgata atcatgaagt atctagtatt
    20521 ttaataggtg tatccaataa agaaagtatg aatgagattc ttaatataga taactttaat
    20581 ggagaaggaa ctattgaaat tattaatagt aagggaaaac ctcttttcat aggaaaaaat
    20641 actgaattaa taaaagacct tagccataaa tataaggatt ctacagatga accttgggca
    20701 caaaagatgc ttaatgattt taaaaatgat aaatcaggga atattacaat aacctcatca
    20761 aaaggtgtac aatgcttatt gacttatcat ccaattacaa gaggtttaaa tgattggcat
    20821 tttcttctca tagtacctga agatgctgta cttggtgaac ttaataagtt aaattctttt
    20881 acaataacaa tgacatttat aattacatgt attattggaa ttataacatt aattttatat
    20941 attataagaa gaaaatatgt aaatcaaata gaaaatattg catatttaga tagtataaca
    21001 aatggcataa attcaactaa gtttagtatg ctagtcaaac ctttaatttc agaatcacct
    21061 gatagtactt atatgatgat agcaatgaat ataaaagatt ttaagctaat aaatgattgt
    21121 ttcagtagtg aaaagggaaa tatgacacta aaacatcttt acaatatttt gtataaaaac
    21181 attgatgaaa atgaagaatt tgtgtgccgt catgatgcag accttttcta tttgcttatt
    21241 aagaatagac cagtgttaga agccctggag ttattatata aaatagaagc tgacacaaat
    21301 tattttaata aaaataggga aaatccatac tttttaagac tatcagtagg tatttacaca
    21361 attgaaaatc ataatgaaga tttaattaca atacaagacc atgcaaatac agcaagaaaa
    21421 agctttaata aaactcatca aagtgatttt tctttttata gtgatataga aagaaaacga
    21481 ttaatatctg aaaaggaaat ttcaaattta atggaaaaag cccttgaaaa taaagaattt
    21541 tttatgtgct tacaacccaa aattgatata aaaagtggaa aaattagtgg agcagagtct
    21601 cttgtgcgtt ggaaaaattc agaaaagggc ataatttatc caagtgattt tattcctctt
    21661 ttcgaaaaaa gtggatttat atgtaagtta gatttatata tactagaaga aacatgcaaa
    21721 ttgatttcta agtggataaa agaaggtaaa gaagtgttga gaatttctgt taatgtatca
    21781 cgtcaacatc tgaatgataa attgttttta gaaaaatata agcatatatg caataaatac
    21841 aatgtaccaa catgtctgat agatttagaa cttaccgaat ctattttctt agaaaatcca
    21901 gaagctatag atataataga ggatattcat tctaatggat ttaaatgttc tatagacgat
    21961 tttggatttg ggtactcatc attaggaata ttaaaggatt ttaaagttga tataattaag
    22021 ttagaccgtt cattctttgt tagcaaaaat aatatagacc gtggtaaggt agttattaag
    22081 tctattattg aacttagcaa aaggctaggt atgaaagtaa ctgctgaagg aattgaagaa
    22141 ttagaacaag ttgagtttct aaaaaatgtg ggatgtgatt atattcaagg ttatgttttt
    22201 tcaaaaccat tattaatttc tgattttgaa aattttgcat atgaaaataa taaaattaaa
    22261 aatatccatt attaaatgta caagataaaa aatttaggca agttatataa ttaaaaatat
    22321 aacttgtctt ttttatttat atatttaata aaaatatatt tgcagatttt gtaaaaataa
    22381 ttttcaaatg ttgtaagttg tggctaaagc tttattgtat tattctgaat atagaaagat
    22441 agttactgtc ttagataaaa actgaaacat acataataat ataaaatttg ctatattaat
    22501 aactctaatt attttggaag gagggaaaat atgataagtg ggagaatgtt actcatcatg
    22561 aattttctaa aaggaaaaaa taagacaagt tacaaggata ttagcaaaca acttagtata
    22621 gaagaaagaa aagttagata tgatataaat aatttaaata ttgttttgcg ttcattaaac
    22681 aagtcagtaa tacaaaagat gaacaaaggt gtgttaatta ttcctgatga cttccaagta
    22741 gtatgtataa ataataacga atatgtattt tccttaactg agcgagtatc tattatgaaa
    22801 attatatcaa tgtttaaaat agataatcta aacttagaaa aattaagtaa agaatttcag
    22861 gtttctagaa gttcaattaa aaatgacctt aatgttttgt caagtgaatt agaaagaaac
    22921 caaataacta ttacttatga taaatctttt aaaataagtg gagaagaaga cattatatat
    22981 aagcaaagat tgaacatatt aaaatcgtat tcttatcttt ttggtaagga aaaatctgat
    23041 ttaagtgtat ttgagaagta tttagtgaat gtaattgaaa aaatattcaa agggatttat
    23101 ttgattgata tatataattg gtctatgaat ttattattgc aaatgggatg gactttaaat
    23161 gatgaatctt ttacatggta tgtgtcaaat attttccttt ttacatggta tatacattca
    23221 gatgaaaaac atcctttaga atctgcttca ttaagtgagc cgtgttttga aaataagttt
    23281 gaccaagaat ttgaaacaat tattcaaaaa aagttgacta aggaacaaat ttttctgtta
    23341 aagagttttg tattttttac aaataaatat gcaagtttaa acgaggatat ggatttgatt
    23401 agtacagaaa ttatagttca agatctaatt tacaatatgt cagaaatatt agagattagc
    23461 tttaaagaag atatgattct ttataaagga ttactcaatc atattgcccc attgatagag
    23521 agaatcaaac agaatataca gatttatgag gaattacttc atgtaatacc agataaatat
    23581 caatatgtac ttgaagctac taaggtttct attcaaaaaa acagagtttt aagtcaaata
    23641 aagaatgaaa atgaaacaat acttttggca atacatttct taggaagtat acatagaaat
    23701 gagaggaata actacaaaaa tatattgtta gtttgtggat ttggatatgg tgtaattgct
    23761 atgttaaagg atagtttagt aaatgattac cagataaata tagtagagag tattccatca
    23821 tataagttac aagattataa agaatgggaa agtattgact tcattataac aacatcaaag
    23881 atagtaatag acttgcctaa aacaattatt gagataaatc catttttaca agaagaggat
    23941 tatttgaagt ttgaagaaaa agggataaaa agaaagaacg tcttaactaa ttatatgtca
    24001 ataaaaaaaa ggttggactt tttagaagaa gatgcccaaa aaagagtatt aagtattata
    24061 tgtcaagaac ttggatacag tgatgagaga atattattta gacaacttaa tttaagtgat
    24121 ttaataggga ttgataccat aaggataatt gatggagata tgaaatggga agatgctgta
    24181 aaatttagtt caaacatact tgctaattgt gattttgtat cagcacaata tgctgagaat
    24241 attatagata ttttaaaaaa tgtgggtttt tatgctgtaa aagataacga atttgcttta
    24301 ctacatggca atgatagttc attagtcaaa gtaagttcga taagtttatt aatatgtaaa
    24361 gaagcagtta gttttggaga taaaaaggtt aaaatcatat tttgcctagc aagcagagat
    24421 aaaaaagagc aaattcctgc aataattaat ctaactaaaa tggtttataa gactaatttt
    24481 attgctatgt tagaaagtgc aaagacaaaa gaagaagcag aagttttaat tcataaatat
    24541 gagaaggagg tcactgtatg aataaattag ttaatgtaaa atgttcattt aaaatacatg
    24601 ttaattcaaa agaagaagcc attatatcgt tagttgatgt agtgtcaaaa gaaggatatt
    24661 taatagataa aaatcaattt ttaaaagatg ttttaaagag agaagaaaca ttgtcgactt
    24721 atattggaca tggtataggt cttcctcata gtcaaagtgt aggtgttaaa aactcttgta
    24781 ttacaatagg aaagcttgat actcctattg aatggacaga agaaggagaa aaagtggatt
    24841 taatattttt gatatcagta acaaaagaca atgaaaataa tcttcattta aagattttat
    24901 caaaattagc acgtttattg atgcatgaaa gttttagaaa tcaaattaga tgttcagatg
    24961 aacaaactgt ttataatctt ataaaagaaa aaatagagga ggaagattaa aatgagaatt
    25021 ttaaagtttt tgcaaaaaca tcttatgaca gcaacatctt atatgattcc atttgtggtt
    25081 gcaggaggaa tattatttgc tttatcagta actctaagtg gacaagctgc tgttcctgaa
    25141 acaggctggt tggctaaact gaatcaaatt ggagctgctg gcttggcttt attcattcct
    25201 atattaggtg gatatatagc atttagtatg gcagataaac caggattagc tccaggtatg
    25261 ataggtgcat atttagcaaa agaagtaaat gcaggtttta taggtggtat catagcagga
    25321 tttatagcag gatttgttgt tttacaatta aagaaaatta aattagctcc tacaatgaga
    25381 actttaggtt ctatatttat ttatcctttg attggtacat tgattacagg tggtataatt
    25441 gtatttttaa ttggagaacc tatagcttct tttatgacat ggatgacaaa ctggttaaat
    25501 ggaatgtctg gtgtttctaa aattccttta ggtggaatct taggaggaat gatagcctca
    25561 gatatgggtg gtcctataaa taaggttgct gctacttttg cccaaactca agttgacaca
    25621 cttccatatt taatgggtgg agttggcgtt gcaatttgta tacctccaat tggtttagga
    25681 ttagctacat tattgtttcc aaaaaaattt agcaaggaag aaagagattc aggtaaagct
    25741 tcactattaa tgggatgtgt tggaattaca gaaggagcta ttccatttgc aacatcagat
    25801 ccagttagag taataccttg tattatggct gggtcgatta taggaaatat tatggctttt
    25861 ttattaggat gtttaaatca tgcaccctgg ggaggcctga tagtattacc tgtagttgac
    25921 aataggttgg ggtatattgc atcagtatta actggagcag tagttgttgc agtattaatg
    25981 aaattagtaa aaaaagacgt gaaagaagat gaagaaattg aagaagattt agatgaatca
    26041 attgaattaa tatttgaaga attataaata taaatggaag gaatttgtat aaaaaagtaa
    26101 atataaaact ataaatttaa aggagataag aatatgaaaa ttgtagcagt aacatcttgt
    26161 cctagtggag tagcacatac ttacatgtcg gctgaatcac ttgaattatc agccaaaaaa
    26221 tttggtatag aaataaaggt tgagactcaa ggaagttctg gaatagataa tgaattgtca
    26281 cttaaagata ttgatgaagc aacttgtgta atattgacta atgatgttga gattagaaac
    26341 atggaacgtt ttaagggaaa gaaggtagta agaatgagtg tatctgagat aattaagaaa
    26401 tctgatgcat taattaagaa aataaaagat acttttcaat aaaaattgat ttattttaca
    26461 tataactcaa agatgttttt aaagaaattt ttattaaaag catctttgaa aatatcttaa
    26521 aaatagaata aaaggagata tagtcatgaa aaagttacca ataaagactg tcgtacaaag
    26581 attactaaac ttacaagaag aggggaaaag tgcaactctt ttaggaattg gtcctatgtc
    26641 accaaacttg ttacaagcaa gttttgaact agcaaaagat gatgattttc ctttaatgtt
    26701 tatcgcaagt agaaatcaag tagatgcaga tgaactaggt ggcggttatg taaatggttg
    26761 gaatcaggaa acttttacaa aagatattaa gaaggttgcc gacaaagttg gatttgatgg
    26821 tttatattat ttatgtagag accatggtgg gccatggcaa agagataaag agagaaatga
    26881 ccatttgcct gttgatgaag caatggagtt aggaaaaaaa tcttatttag cggacattga
    26941 agcaggtttt gacctattga tgattgaccc tacaaaagac ccatttgaaa ttggaaaagt
    27001 aattccacta gatgtagtac tagaaagaac tgtagaatta attgagtatt gtgaaaacga
    27061 gagaaaaaga cttaatctac cagacatagg ttatgaggtt ggtacagagg aaaccaatgg
    27121 aggtctgact tcaactgaga cgtatgaaac atttataact agactaaagg ttgaacttga
    27181 taatagaggt ttgccaatgc caacatttat agttggtcaa actggtactt taactagaaa
    27241 gacagaacaa gtaggaattt ttaattttag aaatgcttat gatttagctc aaatggctaa
    27301 gaaatatgga gttggtttaa aagagcataa tggcgattac ttagatgatg taactttatt
    27361 agaacatata ccatctcaaa ttattgctac aaatgtagct cctcaatatg gtactgaaga
    27421 aactagggca tatttaaaat tagcagaagt tgagtgtaaa ttagaaaaag agggcttagt
    27481 tgagaaaact tctaatatca gacatgttct tcttgttaat gcaattgaat gtggacgttg
    27541 gagaaaatgg gtagtaggag aacaaaaaaa tctaactact gaagagatat ttaaggatga
    27601 agttttatct aatgaaatac tagatattgc aggacattat acatttaata atgatgatgt
    27661 taaaaaagaa atagaagtgc tttatgataa tttaagtaag aataatattg atggtcaaag
    27721 atttgttgta gaccatatta aacgtccttt gcgtgattat gcagaatgct ataacttaaa
    27781 aggtgttact acgaggattt taaataaata ataggactta tagtggtgag tattgttata
    27841 ctaggtattt atagttataa ttatacttac cactattaaa tttaggagga ctaatatttt
    27901 gattaattta atttttgatg tagatgatac tttatataat caattaacgc ctttttacac
    27961 tgcatataat aaagtgtttt cttcaataaa ggatatttct attgaagatt tatatatgag
    28021 tagtagaaag tatagtgatg aggtatttca tatgactgaa aatggagaaa tgccaatcaa
    28081 agaaatgcat atttatagga taatgaaagc ttttgaggag ttgggaaatt ctattacaga
    28141 aaaagatgca caaagttttc aagatgagta tatatatcaa caatcacaaa ttacactaat
    28201 accagaagtt gagaggattt taaatttttc taaagaaaga aatattaatc taggaattat
    28261 aactaatggc ccatcaaacc atcaaagaat gaagttaaag cagttaaata ttgaaaactg
    28321 ggttgataaa agtaatattt ttatttctag tgaggttgga ttttctaagc cagatactaa
    28381 tatttttaga gtagctgaga atgttatgaa tttagataga gaaaatacat attatgtggg
    28441 tgattcttat agaaatgatg tattaggtgc taaaaaagct ggttggaaga gtatatggtt
    28501 gaaccataga ggatatgagg tagaagaatt gttttataaa cctgattttg taattttgga
    28561 acataaggat ttgatatctt tatttataaa aatatgtagt tataaaaata tgtagtaaaa
    28621 aaatgttaat aatagaattg tataaaataa ttaataataa ctatataaaa ttatatgttt
    28681 agattgtgag caatctattt tatattaaaa atagattatt tagaagaagg ggaaatctta
    28741 aaaaaatttg aatatcccct ttttattatt taattaaaat gaaatagtta gaaatatatt
    28801 ttatttagtt tagtcaataa cattttggga ttctttataa ctacatgtta actgttgaat
    28861 aggtttatca tatggtttaa cccatccttt attaatactt aattctgtaa gtgctgtgtg
    28921 accttctacc atttgagtta atgaagctga atatatagct cttaattcag gtgtactact
    28981 tgtcaatgca gaattaagat acatattggc agatgcttta gcagatgaaa gcatacttaa
    29041 cattattact ttatcgtcta tataagtatt gttcttaatt agattttcta tcatatttct
    29101 catgttatct atacctcctc agaaattagt atttcatttt caactataaa ttgttgtata
    29161 gctttaattc ttccttctgt tgctaatata ctagattctg attgtctttt taggtcttca
    29221 tctgatatta acatatttat tgctctttgc ataagtaatc cgtcagactc catttttata
    29281 attgctgcta aagatatttt ttcagtttct gaaagtattc tcatttaatc atctcctata
    29341 ttgtaattta tgctttaata gtatgtcttt cttttcaagg aaataaccat tacacttttt
    29401 tacagtattt tatattctct ttatatcaaa atttagtcga cctaatatat taatataatt
    29461 ttgtaggata tttatcatta aatatctata aaataaatta tttgaatatt cataaagcaa
    29521 aatttatttg tatgtacttc ttcaagagtc aatatgagtt ttttcaaaaa attaattttt
    29581 atgataatca ttaagggcta tatttttctt aaagttgtga ttatcattat taatttacaa
    29641 taaaattgcc gaaaagccct ctcctttagg tgtagggatg aaaggtaagg tcttaatata
    29701 aaaaaagtta gaataatgct tgtaatctaa taccgctact gataccctat atttaggcgg
    29761 tgttagtttt tatgtataaa tctaacaaaa atatagttta tgattgcaaa tatcatattg
    29821 tattttgccc taagtatcgt aaacctgttt tagttggcga agttgaaaag atgttaaaag
    29881 aaatactacc atataaagca gatgaacttg gagcagaaat aatagaaatg gaaactgata
    29941 aagaacatgt gcacttatta atatcttgtg accctcaata tggaattcat aaagtagtaa
    30001 aaggtttaaa aggctttagt tcgagagtat taagagagaa ctttccacat ttaaagtcta
    30061 gtatgcctag catgtggact aatagctatt ttgtaggtac tgttggttct gtgagtttag
    30121 aagtagttaa acaatatata gaaaatcaac aagttaggac aaagaaatga taaaaactca
    30181 tagagtaaaa ttaaacctaa ctagaacaca atttgaatta gtaagggaaa agcaaatgga
    30241 aagtgctaac tgttggaact acatagttaa cttatctaaa gaatactatt ttgaacataa
    30301 acaatggatt aaaaagaatg atatacaaaa gttaatcaaa ggtaagtaca atcttcatag
    30361 tcaaactatt caagctatat cagataaatt tgatgcaaat agaaagacta tatcagaatt
    30421 aagaaaaaaa ggtaatacta aagcaaaata tccatacaag actaagaaat tttatataat
    30481 accttttaaa gctagtgcta ttaatagaaa tgctaaaggc aacttaaaat tatcaatgtc
    30541 aaaaggcagg tatttagaat tagattttaa tgttgagaat ataaaaactg ctgaaatagt
    30601 atggcgaaat ggatattatt tatactacac ttttgacaat gaattaaatg gagttgtagc
    30661 caaaggcact aataccgtag gtgtagactt aggggaaata cattcgatag ctagtgtaac
    30721 aaatgaaggt gtagggctta ttctatctaa tagagaaggc agaagtatta aacaatttag
    30781 aaataagatg tatgcttata tatctaaaag attaagtaaa tgtaaaaaag gtagtagaca
    30841 atcaaagaag ttatggagat taaaaaataa gataagaagt aaaacagata atcagttaat
    30901 gaatttatat catcaaacaa ctagaaagtt tatagacttt tgtgtagagc agaaggtatg
    30961 tgaaatagtt cttggggata taaaaggggt tgaaaaagat actaagaaaa agaaaagatt
    31021 aaacagagtt aatagacaga aaatatctca aatggagtat ggtcgaataa aggactatat
    31081 aaaatataaa gcaaaagagc aaggtattga agttaagtta gttaaagaaa actacacttc
    31141 tcaaacttgc ccaaagtgtt ctaaaaaaca caaaccaact ggtagaactt atagttgtac
    31201 ttgtggttat gaaactcata gagatatagt tggtgcttgg aatattttaa ataaaaagca
    31261 taaatatggt ttagtagatt ttagaataaa tcataagcaa ccaataaatt taaaagtatc
    31321 taccgtttga gccatgtgtg ggcgaataac tttagggtag tagtggcgaa gacaccgtcc
    31381 gaagtagctt ggctttgtaa ccacctctgt tgtactgttt aagtataaat aagtgtgtta
    31441 gccaccaaga atccccttgc tttagctatg gggagtgtgt caaatattaa acatagcttt
    31501 tatacattta ataaggtatg taaaatctat gtaaaaagta agaaatataa acattttttt
    31561 aattatattt taaaaatatc aagcaagttt tcttcacaaa tgaataaaaa atgttataat
    31621 atgattaaag aaaaagataa ataattagca agatttgttt agctaaaatt aacaagggag
    31681 cgataaatta tgggtataac attatcaaaa ggccaaaagg ttgatttaac taaaggaaat
    31741 ccaggtctta aaaatatttt agtaggttta ggatgggata ctaacaagta tgatggtgga
    31801 tttgactttg atttagatac agcagcattt ttaacaggag caagtggaag tgttactaat
    31861 gatggagatt ttgtatttta caacaattta aaacatacat ctggagcagt tgagcatcta
    31921 ggagataaca gaactggcga aggtgatgga gatgatgaac aaatagttgt tgatttatct
    31981 aagattcctg gagaaataag caagatttct tttactgtta caattcatga tgcaacagaa
    32041 agaagacaaa attttggaca agtaagtaat tcttatataa gaatagtaga taaagacaca
    32101 aatgaagagt taataaagta tgaattagga gaagatttta gtatagaaac agctatagta
    32161 gtagctgaaa tatataagca caatggagaa tggaagttta atgcactagg ttctggcttt
    32221 gaaggtggtc ttgctgcatt atgtggaaac tttggaataa acttataatt tcaactataa
    32281 caataaacta aagggggata ttttccatgg ctatagtatt aaaaaaagga caaaaaatcg
    32341 atttaactaa gggaaatcca ggtcttaaaa atataaaact tggactagga tgggatacaa
    32401 attcatttga tagtggctat gattatgatt tagatgtaag tgttttcatg gttggagaat
    32461 ctcaaagagt tgaaaaagat gaagatttta tattctacaa taacttaaaa catccatctg
    32521 gagcagttga acacttaggt gataatagaa ctggtgaagg tgatggagat gatgaggaaa
    32581 tattagtgaa tttagagaca atgccaaagc atatacaaaa gatagctgta acagttacaa
    32641 tatatgaggc tgctgaaaga agacaaaatt ttggacaagt aagtaactca tatataagag
    32701 tattaaattc tgaaaatgat gaagaaatat taagatatga cttaggtgaa gaatttagta
    32761 tagaaactgc aattacagta tgtgagatat ataagtataa tggagaatgg aagtttagtg
    32821 cagttggagc aggatttgaa ggtggcttag aagcactttg tagaaactat ggcttaaatg
    32881 tttaatttaa gcaatataat atattaaata taaataacct atacaaataa tgtataggtt
    32941 atattatata aaattttgtg gaggtttagt atgtcgatta atttatcaaa aggtgataaa
    33001 atagatttaa aaaaatctaa cccaggactg agtaacatac ttgtaggtct aggatgggac
    33061 cctgtgcaac aatctggtgg aggttttttt aaatcgttat ttggtggagg acaagctgat
    33121 atagattgtg atgcatctgt atttatgctt aatcaagaag gtaaattaag tggaataaaa
    33181 gatttaattt attttggaaa tctaaaaagt gcctgtaaat cagttttgca tacaggagac
    33241 aatctaactg gtgagggtac aggtgatgat gaacaaatcc tagttaattt ggataaagtt
    33301 ccatcaaata ttcataaatt attgtttgta gtaaatatat acaattgtgt agatagaaaa
    33361 caacactttg gaatgataga aaatgcctat ataagagttg aagaccaagg taataagaaa
    33421 gagatagcaa aatacaatct atctgataat tattctgaaa aaactactct tatagtagga
    33481 gctatatata gaaaagatgg aagttggcaa tttaaagcaa ttggtgaagg aacaaaagat
    33541 gcgggtctta aagaagtaat gcaaaattta gacaggattg agtgtgcata tggaatttaa
    33601 taatgatatc aaatattata atgtagataa agaagaagtt acaaaagact tatctgttaa
    33661 tcctgaaaaa ggtttatcag aatcagaagt caagactaga agagaaaaat atggtttgaa
    33721 tgaatttact ccaaaagaag agggtagttt ttgggatgat ttaaaggaaa gtttaagtga
    33781 accaatgata gttatcttga ttattgcagc agtagtaagt gcagtaattg gggaaactca
    33841 tgatgcaata gggattgtag gtgctatagc aataggtata gcaataggta tgattacaga
    33901 aggtcgttct aagaaagcag cagaggctct ttctaagttg actgaaaaca tagaagtaaa
    33961 agtattgaga gatggggaag tacatcaaat ttctaagagt gaattggtac caggtgatat
    34021 agtatatatt gaaactggtg acatggtacc tgctgatggt agacttatag aaagtataaa
    34081 ccttaaaatt agagaagata tgttaacagg agaatctgat gacgtatcta aaaagtgtga
    34141 tgtagtagtt tctatggaag agatagaatc taaaggagca gtagtagtac aggagccaat
    34201 acctgcaaaa cagataaata tggtgtttgg tggcacactt gtagcttatg gtagaggagc
    34261 tctagttgta acttcaattg gagataatag tgaaatgggt aaaattgctc aaaacttatc
    34321 agaaacagat gagcaaactc cacttcagat aaaacttgga aatttaggtg gtttgatagc
    34381 caaagtttct agtgcagtag caggattact atttatattt atggttttcc aaatgataag
    34441 taagaatgta ttaaatgtag atatgtctgg aatattaccg tttttagaat caatagaccc
    34501 agttaaaaca gcatttacag tatgtgttgc gttgatagtt gcagcagtgc cagaaggtct
    34561 tccaactatg ataaatatga cacttgcaat aacaatgcaa aaaatggcta aaataaatgc
    34621 attggttact aaaaaagaag cttgtgaaac gataggttct gtttctgtaa tatgttcaga
    34681 taaaactggt acattaactg aaaatagaat gactgtagaa gttgcatatg ttgatggcag
    34741 atatattgaa agttcagaag aggaaataaa tagttatttt gaagaaaact gtatgataaa
    34801 ctctacagct gatgtagagc acaatgatgg tgatattaag tatttaggaa gtgctactga
    34861 atgtgcattg cttttatatt acaaaaacgt agattataga caagttagaa aaaattctaa
    34921 tatagtagct caaaatcctt ttacttcaga ttcaaagaga atgacttctg ttataggtca
    34981 agataatcat catgtactat tatcaaaagg agcaccagaa gtcttacttg aattatgttc
    35041 acatattcaa agaggaaaag acatagttcc attaactgaa aatataaaac atgagatttt
    35101 agaagaaata aagaaattgc aaataaaatc aatgagaact ttagggtttg catataaaga
    35161 aatctcacaa gctgaagaag aagcagcagt aactgctgaa agtgatgcta tgaatgtaag
    35221 tgcaatggaa aacaatttag tatttagtgg ttttgttggt ataagagacc cacttagaaa
    35281 agatgttata gagtctgtaa atacagcaaa taaagctggt gtttctgtaa aaatgcttac
    35341 aggagataat ataaatacgg caagagctat aggtgaagag ttaggcctat taaaaaataa
    35401 tatgagagtg gttgaagctt catatataga tactttagat gatgaagaat taaaacaaga
    35461 gatacaaagt atttcaatcg tggcaagaag taaaccagat agtaaaatga gaatagtttc
    35521 agctctgcaa aagagcggag atgttgttgc agtaacaggt gatggtataa atgatgctcc
    35581 agcactttca aaagcagatg ttggtatagc aatgggtata tctggtacag aggtatctaa
    35641 aaacgcagca gacataattt taactgatga tagttttagt accattgtaa aaggtattaa
    35701 atggggtaga ggaatttatg ataacttcca aagattcgtt caattccaat taactgtaaa
    35761 tgtaattgca tttttagtgg ctataatatc tcaagttatg ggacaagaga tgccatttac
    35821 tacaatacaa ttattatggg taaacatcat tatggatgga ccaccagcac tagcattagg
    35881 tttagagcca gttagggacc atgtgttaaa tagaaaacct gttgatagaa aagccaatat
    35941 aatttctaag tcaatggtgt atacaatagt attaaatgca ttttatataa cagcaatact
    36001 aatgttacaa tctacattta atttcttagg agttgatgaa gcgcataagg gaacagtaat
    36061 gtttgcgtta tttgctttta gtgcactgtt taatgcattt aactgtagag agtttaatgc
    36121 agatagtatc ttccctaatt ttacacataa caaattagca ctacaaataa tagtgttgac
    36181 aggaattgca caagtaatat ttactcagct attccaagat ttctttaact ctgtagcaat
    36241 ggattttatg acatgggtaa aagttttatg tgtagcagca agtgtaattg tggttaatga
    36301 agttgtaaaa tgtataataa gactggtaaa actaaaaaag actgcttaaa acaaattttt
    36361 tagaagaaaa cagactgact caaaatatga attttgggtc agttctgaat aaacatttaa
    36421 attgggagga tagttatggg tgactcaaag gtatttgaaa aaatattctc cagccaatca
    36481 gatagaggaa attacactcc ttcaaaggga tatctaagtt attttatttc gtatattggt
    36541 ttagaagatg aagttttgta caatctcgag atatttaaga caaaacaaaa tattgatagc
    36601 aaaaaagata ttgctttatt tactgatgtt atagcaaatc ctagtgattt tgatattata
    36661 aattatttta aaagtggact tcaaaaatat agaactagca tggaagatgt agatataaat
    36721 attttgggat ttgaagagat tgattataaa ataaagcaag caatggatag agtgttaaaa
    36781 gaagaggaaa aagaatttac taatgataga gttaaacaaa attttatagt gaaaataatg
    36841 gcatggataa aaatttatat cggtgcttta gatataaata aaaatgaagc tccaaaagta
    36901 atattttatg gagatataaa aaaacatgaa gtttatttac tcttaatatt atacttggca
    36961 ggttttgatg ttttatatct taatccaaat tcaaagagta atatagatat tttaaaatca
    37021 gaaagataca atatagagtt tgaagaagcc aacataatag aagaaaaaat ttcttttgaa
    37081 gaaagggtta ttttagggga aaaaatagat aaaagttctg taaaaaaagc ttttactgtt
    37141 ggggcagaag catcaaagcg tataagtgag gaattgttaa atgatgcagg atttataaaa
    37201 ccttggcaac tccaagatag aaaaataaaa aatctacttc taagttcaac tgttgatgaa
    37261 atatcaatat actggaatca accacttaaa ttaagaccag gatttaagtt taatgatgct
    37321 attgtagaag ctccaaattt tctttctaag attaatggaa tatataacga taagaatgaa
    37381 tatattaagt ttttagattt attaagagat tcggaaagtt caacatttat tgaatttaat
    37441 ggagatgtag atagattttc aaaagctttt acaagagaag cttttagttt atccttttta
    37501 ttggattcaa aaggtgcaat agataaaaac tctgttttaa ataataaaga ttattccata
    37561 tcaacactag cattgaatca acaaattatg attttagaga aagtggaaga gttgttagaa
    37621 ggaagtatgt ttttgaatgg tttaagtgga gaagacaaga taaaaggtct atttacagta
    37681 cttcacatgg ataagaaatt tgttcatatg atgaacaatt tcgattactc acttataaac
    37741 ccaaaattga ttatatacat gtacaagagt attgtatttg ataaagaaat agtttttctt
    37801 atgcttttac ttagtaagat tggatttgat attatcatat tatgtccagg aggagaaaac
    37861 aatatagaga atgttataaa taaccaattg attgatgtac acagattgga taaaatggta
    37921 tatgacctta aattaaattc gcttgaaaat gatatacctt tacttaaaaa aatttttgga
    37981 aagaggagac gattttagat ggggataaat ataaatagtt tttcaaaaga taagaactct
    38041 gtagaggtta ctgaaaattt gcctacagca gttcaagaag aaaacttcga tataatggaa
    38101 tatacaaata ataaaaaaaa tgaactgaga aagtctaagg aagttgaggc attgacatct
    38161 cttatagaag tagaaaatcc agatacgatt ttacagtttg gtaggaaagc atctgaagga
    38221 gtagcaagag tttctgatag ccttttaaat acaataaaat taaatagaaa tgaagaaaat
    38281 tctaaaatgt tggttcattt aactaaaata atggacaagt ttgatttaga cgattttcaa
    38341 gaaactaagg aaccaaattt cgttcaaaaa cttttcaaaa aagctaataa tgccattgaa
    38401 atgatgtttc aaaagtatga aacattggga ggagaagttg agaaaattca aattgagctt
    38461 gaacaatacg aaagagatat agcactttct aataagcaaa taggtgctat gttaaatgaa
    38521 aactttgagt tttacaatga acttcaaaaa tatattgttg ctggagaaat ggcaatagaa
    38581 gaaatggata atgaaatact acctttcttt aaacaaaaat cagagactag tggagaccaa
    38641 atggatgttg tcaattatca agaactatta aaagtttatg atatgttaaa tcaaagagtc
    38701 tatgatttaa ggatagcaga aaatatagct atccaaacaa tacctatgct tagaggtatg
    38761 caacataata attatggttt aattagaaaa ataaatagtg catttgtcgt aactttacca
    38821 gtatttaaac aatgtttatc tcaagctata ttacttaaaa agcaagaatt acaagcaaaa
    38881 tctctaaaag cacttgatga caaaactaat gaattattgc ttagaaatgc acaaaatgta
    38941 agtactcaaa gtgcacaaat agctagaatg gctggaacaa gttctgttca aatagaaaca
    39001 ttggaaaaaa tgtacaatac tatcaagtct ggtatagatg aaactatgag aatagaagaa
    39061 aacaatagag ctttaattaa ggataataca aagagattgg aagaattaaa tacgactata
    39121 atttataata agtagtatta tattaagggc taatttgtat aaagttggcc ctttctatgt
    39181 atatgaggag gctagacatt gaaatatttc gattatatct gcaaagatga tttggagaga
    39241 atttttttga aggagccaga agattttagt gctaagactg aaaaagatgt gttgaaatat
    39301 gcactgggcg cttttttata cgttccagct actcaatata atatgattta taagagtatt
    39361 ataggggatg ttaagggagt tagaccatta gctatttgtt tagaagatgc tgttggagta
    39421 aatggagagt tggaggctat agaaaatctt agattaattt taaaaaatat tagcaatgaa
    39481 agtataacta ataaagatgg aataccacta atttttgtaa gaataaaaga tgtggagcaa
    39541 ctgttgagaa ttaaagagat tataattaaa aatagtcatt ttataacagg aatattaatt
    39601 cctaaagcaa attcggagtt aattgaaaat tgtatagaag ctttagattt tatgaatctt
    39661 caagatatgt atgttattcc gatagtagag acaaaagaat ttatatataa tgaaaaaaaa
    39721 gaacttagtt ttactaattt gtacaatgct attttaagac ataagtcaag aatattaagc
    39781 attagagtag gcataactga tatattaggg atgtatggta taaggagaga taaaaacttt
    39841 tctatatata ataatttgat ttgttcttca tttatattag atataatcac atatcttcaa
    39901 agaccagagt tagatatacc tataagtggt ggagtatcag aattttttga tatgacgaat
    39961 aaggatataa gaaataagta tatagaagaa atattattgg ataaatatca tggattaatt
    40021 ggaaagaccg taattcatcc aatgcaaatt caaattgtac aagcgctatc tacagtatca
    40081 tatgaagatt ttactgatgc tttggatata ctggatagta cagacagtaa gtatggtgtg
    40141 agcaagggtg tattaggaga gagaatgaat gaaacaaatc cacatttttt atgggctaaa
    40201 aaaacattga ttctatctaa aatatatggt gtattaaata aaggagtaga ctatgaagaa
    40261 ttgcttaaat tttaattgtg agattgacat tattgataat ccattgaatt tagaactaaa
    40321 tgatttttta gacgtaggaa taagaaataa tagcaaaaga cgttttttgt ttatctcaaa
    40381 gaaactcggt aaacatttag catgtaagcc aagtgagatg gataatttgg gaaaattaat
    40441 ggctacaatt tataatgaaa aaaataagga agaatcaaat ggtactgtta tagcttttgc
    40501 tgaaactggt acagctgttg ggcatagttt ttttgattac ttatgtggag attatgaatt
    40561 tattcatact actagagagc agtttgacga gttagaaaat ctggaatttt tagaggaaca
    40621 ttcccatgca acagaccaga atttatactt tggtatgctt aacaacttta aatttggaga
    40681 tgagataata ttggttgatg atgagattac cacagccaat acatgcatga atataattag
    40741 gaaaattcaa agcatttatc ctaaaaagaa atacactata tgctcaatac tcaattgggt
    40801 aagtgatgaa aatttaagta aagttactcg tttagaaaga gagctagatt gtaagataga
    40861 ttttgtatac ttatttaaag gaaactttaa attttatata gatgaagata ttatttcaaa
    40921 ttacaacgat aattatagta ttactaaatt agagactatt tcaagtgata aagtaaattt
    40981 agtagtaaat catatttatg ttgatttaga agattatatt ggaaataaga aatatgtaaa
    41041 atatacagga agatttggaa ttaatagaag agaacaattt agattaaaag aaatcgttaa
    41101 gagagaaagt aaaaaaataa ctatggccag taaagaatct ttaaaaaatg aaaaagaaaa
    41161 gattctattt ttaggtac
//
LOCUS       DAELNY010000019        38108 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.019, whole genome shotgun sequence.
ACCESSION   DAELNY010000019 DAELNY010000000
VERSION     DAELNY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.019"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            375..1592
                     /locus_tag="KPE55_000842"
     CDS             375..1592
                     /locus_tag="KPE55_000842"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG0491938.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETRATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1687..2067
                     /locus_tag="KPE55_000843"
     CDS             1687..2067
                     /locus_tag="KPE55_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0491939.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2351..2833
                     /locus_tag="KPE55_000844"
     CDS             2351..2833
                     /locus_tag="KPE55_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0491940.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2854..3669
                     /locus_tag="KPE55_000845"
     CDS             2854..3669
                     /locus_tag="KPE55_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0491941.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3720..5270
                     /locus_tag="KPE55_000846"
     CDS             3720..5270
                     /locus_tag="KPE55_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0491942.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5367..6719
                     /locus_tag="KPE55_000847"
     CDS             5367..6719
                     /locus_tag="KPE55_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0491943.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6850..7608
                     /locus_tag="KPE55_000848"
     CDS             6850..7608
                     /locus_tag="KPE55_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0491944.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7655..8374
                     /locus_tag="KPE55_000849"
     CDS             7655..8374
                     /locus_tag="KPE55_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0491945.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8498..9898)
                     /locus_tag="KPE55_000850"
     CDS             complement(8498..9898)
                     /locus_tag="KPE55_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG0491946.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9902..10810)
                     /locus_tag="KPE55_000851"
     CDS             complement(9902..10810)
                     /locus_tag="KPE55_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0491947.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            11071..11718
                     /locus_tag="KPE55_000852"
     CDS             11071..11718
                     /locus_tag="KPE55_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0491948.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            12043..14682
                     /locus_tag="KPE55_000853"
     CDS             12043..14682
                     /locus_tag="KPE55_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0491949.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14740..15843)
                     /locus_tag="KPE55_000854"
     CDS             complement(14740..15843)
                     /locus_tag="KPE55_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG0491950.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16204..17025
                     /locus_tag="KPE55_000855"
     CDS             16204..17025
                     /locus_tag="KPE55_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0491951.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    17104..17315
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17402..18718
                     /gene="argH"
                     /locus_tag="KPE55_000856"
     CDS             17402..18718
                     /gene="argH"
                     /locus_tag="KPE55_000856"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG0491952.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18967..19305
                     /locus_tag="KPE55_000857"
     CDS             18967..19305
                     /locus_tag="KPE55_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG0491953.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19432..20721)
                     /locus_tag="KPE55_000858"
     CDS             complement(19432..20721)
                     /locus_tag="KPE55_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0491954.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20801..21505
                     /locus_tag="KPE55_000859"
     CDS             20801..21505
                     /locus_tag="KPE55_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG0491955.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21597..22643
                     /gene="selD"
                     /locus_tag="KPE55_000860"
     CDS             21597..22643
                     /gene="selD"
                     /locus_tag="KPE55_000860"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21645..21647,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG0491956.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22661..24073
                     /locus_tag="KPE55_000861"
     CDS             22661..24073
                     /locus_tag="KPE55_000861"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG0491957.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24187..26070
                     /gene="selB"
                     /locus_tag="KPE55_000862"
     CDS             24187..26070
                     /gene="selB"
                     /locus_tag="KPE55_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG0491958.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26175..28913
                     /locus_tag="KPE55_000863"
     CDS             26175..28913
                     /locus_tag="KPE55_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG0491959.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(29032..29976)
                     /gene="manA"
                     /locus_tag="KPE55_000864"
     CDS             complement(29032..29976)
                     /gene="manA"
                     /locus_tag="KPE55_000864"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG0491960.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            30149..31510
                     /locus_tag="KPE55_000865"
     CDS             30149..31510
                     /locus_tag="KPE55_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0491961.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31598..33487
                     /locus_tag="KPE55_000866"
     CDS             31598..33487
                     /locus_tag="KPE55_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0491962.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33502..33957
                     /locus_tag="KPE55_000867"
     CDS             33502..33957
                     /locus_tag="KPE55_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0491963.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            34016..34333
                     /locus_tag="KPE55_000868"
     CDS             34016..34333
                     /locus_tag="KPE55_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0491964.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34358..35620
                     /locus_tag="KPE55_000869"
     CDS             34358..35620
                     /locus_tag="KPE55_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0491965.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35725..36789
                     /locus_tag="KPE55_000870"
     CDS             35725..36789
                     /locus_tag="KPE55_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0491966.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36843..38021
                     /locus_tag="KPE55_000871"
     CDS             36843..38021
                     /locus_tag="KPE55_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0491967.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT        14975 a         4158 c         6682 g        12293 t
ORIGIN      
        1 aaactttctt ttatattgaa gttgtttagt aagacatgta ttttattaat ttattaatct
       61 taataatgtt aaataaaatc aaaaaaatag attttgggta aataaaataa tataaaatat
      121 aaattttaat aaaaataata aagtatatat tattgcttta ataagaatgt aaaaaatatt
      181 tttaaattat catttaatgt aatttaaatg aattgaagat atatgtttat tgtatgcaat
      241 tgttgtattt ttaggttttt tgtgattaat taaaaaatta taaaaatcgt ttacatgttg
      301 atttttaaaa aaaaaagata tagaattata tagaaagtaa taaacaaaaa ttggatttac
      361 gggagtggaa tgaaatgatg aatgttacct taacagatgt gaaagaggct agagagacca
      421 ttaagaacat agtaaaaaga actgacttac ttgaaagtgt taaacttagc gaaaaaacag
      481 gagcaaatgt gttctataaa tgtgagaatc ttcaaaaaac aggttctttt aaattaagag
      541 gagcttgtaa caaaatagcg agtttaacag atgaagaaaa ggcaaatgga gttatagctt
      601 caagtgcagg gaatcatgct caaggagttg cattgggagc aaaaatgact ggtataaaat
      661 ctacaatagt aatgccagca actgcgcctt tagcaaaagt tagtgcaact aaaggttatg
      721 gagcagaagt agttttaaat ggtgcggttt atgatgatgc atatgcaaaa gctgttgaaa
      781 tacaaaaaga aacaagagca actttcttac atccattcaa tgataagtat gttatagcag
      841 gacaaggaac tatatctctt gaaattttcg aacaattaga caataaagtt gatactatac
      901 tatgcccagt tggtggagga ggaatcatct ctggtgttgc tgttgcagca aaagcattaa
      961 atcctaatgt aaaaatagtc ggtgttcaaa ctgcaaatat accttcaatg aaagagtcaa
     1021 taaaaaatgg aaaagtaaca acagcattta atgatactac aatagctgat ggtatagctg
     1081 ttaaaactcc aggagattta acttttgaaa taataaatga attggttgat gaaatagtag
     1141 tggttgaaga gacagaaatt gcagaatcaa tattatttat gatggaaagt caaaaaatag
     1201 tttcagaagg tgctggtgct gtatgtacag ctgctatatt aagtggtaag tatgtaccag
     1261 caaaagatga aaatgtagtt tgtataatat ctggtggtaa tatagacata aatacgttat
     1321 atagaataat aggtgttgct ttagctaaag aaggaagaag atattcattt agtacaatta
     1381 tggaagataa gccaggaaac tttgctgagt taactagaat aataagtgag aatggtggaa
     1441 acattttaag tgctaatcaa ggtaaattat cagcagggga agctctagga aaacaaagtg
     1501 cggaatttat attagagaca atagattatg accatatagc tagaataaag aaagctatag
     1561 aagaaaaagg ttttaaaatt atagaattat aataaaaagt gtatataata tacaagaaaa
     1621 atagtataat agtgtatgaa gatacatttt atgtgtatta gattaacgaa ttaggaggat
     1681 tttaaaatga aacatgaagt aatacatact aatgatgcac cagcagcttt aggaccatat
     1741 tctcaagcta taaaagctgg aaacttgtta tttgtatctg gacaagttcc tcttgtacca
     1801 gagactatgg aagtagttga aggagatgta caagctcaaa ctgctcaatc tttaaaaaat
     1861 cttaaagcta tattagctga aagtggagct gatttttcaa atgtagttaa gactacagta
     1921 tttataaaag atatgaatga atttggggct ataaacgaag tttatgctga atattttgga
     1981 gaaaataaac cagcaagagc ttgtgtagaa gttgctagat taccaaaaga cgttaaagtt
     2041 gaaatagaag taatagctgt attataaaaa tacaatattt ttaatttgaa aaggctgaaa
     2101 aactatattc tagtgattca gcctttttat aaaaaaataa atcacaataa agaacatgat
     2161 aaatattgtc gaattaaaaa ataaaattaa aataaatatt gcaatatttt tgagaaggtt
     2221 ttataatgag tatataattt gaaacgtgta actattaaat taggcatgag ttcttggata
     2281 tttttatcct gaggattcat gccttttttg tattgaaatt caaaaatcta tttaataagg
     2341 aggaagtact gtgttaaatt ttttcaagaa aaataaaagc tataagttac atgcagtagt
     2401 gagtggtaac agcattaata ttgagaaagt aaacgattct gtgttttcaa aaaagttaat
     2461 gggagatgga gttgcaatta ttcctaatag taatgttgtt gtagcccctt gtaatggtaa
     2521 agtaactgtg cttacagaat caaagcatgc ctttggtatg gtctcagatg agggtgttga
     2581 aatattagtc cacataggaa ttgatacagt tagtttacag ggtgaggggt ttaaaaatga
     2641 agtgtctcaa ggagatactg ttaaaaaagg tagtccaata ataagttttg aaagagaaaa
     2701 aataaattct cagggaattg attgtacaac aataataatt gtattaaatc atagcgaatt
     2761 ttcagaaata aattgcatgg tagaaaatga agttgttgca gggcaagata ctgttattga
     2821 aattatgaaa taataggaag ggattaattc aatatgagag tagtaaaaat ctttaataat
     2881 aatgcattat ctacagtaac taatgatagg aaagaagcta ttttactagg tcttggtata
     2941 ggttttaata aacgtccagg agataaagtt aatgaagata aaatagaaaa gatttattat
     3001 gttcaagacc acatgcaaac aaaattttta gaattattaa aaaatgttag tccagaagtg
     3061 atggatgctt cacaacaaat tataagtctt ataccaggaa atgagggaaa atttaataat
     3121 aaagggatat tatctttagt ggaacatatt agttttgcaa tagatagaat gaaaaataat
     3181 gtttttttac ctaatttaat gcttttggat ataaagatga tgtattcaaa agagtttgaa
     3241 ttaggagtaa aagctttaga aattatatat agagtatgtc atatccattt gcctgaagat
     3301 gaagcaggtt acatagcttt acattttgtc aatttacaat caaatgataa tttggcatat
     3361 gatactttaa aatttgtaaa aggtagtatt gatttgatta aagaatgtta tggattagaa
     3421 ttagacgaat cgagtttaag tacattacgt tttagaactc atcttaaatt tcttgctcaa
     3481 agaattttta agaatgaaat ttgtcaggat gataaaatga ttgaaatgta tgattattta
     3541 attaacaatc atcctaaaaa taaagaatat ttagaaaagt taaatgtata tattgaaaag
     3601 gagtttaggt ataaattaga taaacctgaa aaaatttatc tactactaca cctaacaaaa
     3661 atcttataag aatgagatga attagtaata gaaatatata attttagggg gggataagat
     3721 tgaaagaaaa actacttgga ggactggaaa catttgcaaa ggcaatggtt cagccattaa
     3781 tgtatttatc gagtgcagga atattaatgg tggtaggtgt actacttaca aatgatatgt
     3841 tattaggagt acttccattt tttaaatggg agccaattca aatatttgga agcctaattt
     3901 ataactgtat aatggctatt attaataatc ttgctgttat atttgctata ggtattcctg
     3961 ctgcactagc aaaaaaagag aagcacaaag cagcgttaat aggatttatg agttatataa
     4021 tataccttac agcttccaac actatgttaa catcactagg tcaactagca aaagaaagca
     4081 agatgcttgg actgataggg actggacaag ctactatttt aggtattcaa gttgtagata
     4141 caagtgtatt tggtggaatt attttagggt gtatagctgg atatgtattt aatcgtacaa
     4201 gcaacaaaac atttaaagga gctttacaga tatactcggg agcaaatttt agttttatat
     4261 gcatgttctt tgtatccatt ttaattggag ttgtgagtaa ctttatatgg ccattagctc
     4321 aacaaggaat agcttctctt gcaaatgtca tcaaagatac aggtacattt ggtttattct
     4381 tatatggttt tttagagaga atattagtac caactggact tcatcattta atctatacac
     4441 catttcagtt ttctgattta ggaggagtgc ttaaagttgg tgcagataca gtttcaggag
     4501 cctatgctat agttatgaca gaaatgaatt taccagttga gcgtttctca gatagtatat
     4561 actacatggc aactggattt acaaagatgt ttggttatat aggtatagga gctgccttta
     4621 tttatactgc atataagcaa aataagaaga aagttaaggc aacgattatt ccactaataa
     4681 ttacggcagt attagcctct gtaacagaac cattagactt tatgtttgta ttctgtgcac
     4741 catttttatt ctttgtacat gcagttatag ctggtctttt tatagcttta ttaaaaatat
     4801 ttgatgttac tgcattatgt ggagggaact tattagtatc aattataatg aatactacag
     4861 taggcgtaga aaaaacacac tggccagtaa tgatgatatt aggacttgca caaattattg
     4921 tatattttgt agtatttagt tttttaatta agaaatttaa ttataaaact ccaggtcgtg
     4981 aagatgagag tacagctaca ggcgaagaac aatcaaaaca acttaattta gatgcaggga
     5041 tagaaaatat aattgatggg ttaggcggaa aagaaaatat taatacagta gaaaattgca
     5101 tcactagatt acgtgttaat gtaaaagatg aaagtaagat taatgaagat ataattaatt
     5161 taacgccaaa tagcgggatt gtaaggaaag gaaaagatat acaaataatt tttggacttc
     5221 atgtgcatga ggtaagacgt gcagtagaag attttttaga agcttattaa agatatatat
     5281 ttatatagca agggtaaata acagctttaa gtgctgttta ctatttatta aagatacata
     5341 taatttatag aggaggaata attattatga ttattactat tgttggtgga ggaagcacat
     5401 ttactccagg tattgttaag tcaattgcat taagagaaaa agagttagag cttgaggaaa
     5461 ttcgcttatt tgacattgat aaagaacgcc aagataaagt agctgtagtt gtcaactgga
     5521 tactaaaaga agatgttaaa acagatgtta aactaactgt aactaattgt gaaaaagaag
     5581 cgtatacaga tgctacattt atatttgcac aaatgcgtgt tggtaaatat gaaatgcgtg
     5641 agcaggatga aaaaattcca ttgaaatatg gttgtgtagg tcaagaaact tgtggttgtg
     5701 gagggatggc atacggactg cgtacaatac aaccaatgat aaaggttatt gatgatgtag
     5761 agaaatatgc taaaaaagat tattggatat taaattactc taatcctgct gcaatcgttt
     5821 ctgaagcttg tcgtaagtta agaccaaatg caaggattat aaatatctgt gatatgccaa
     5881 tagctattat agatatgata gcggggtcat taaatatcaa tgacgttcat aatatacgct
     5941 atgattattt tggtctaaat cattttggct ggtttacttc aatagattat aaacatagag
     6001 acttaatgga agagattaag gaatacataa aagaaaataa aatattatta ccaaaatctt
     6061 atatagaaaa cttaaataaa agtcgtagta caggaaatcg ccatgctgta agtagctgga
     6121 tacatgtttg ggaatctgtt tatacaatgt tagaaagctt cccaaaatat ctgccaaaca
     6181 cttatttaac atactatctt ttaagtaaag aaacagtgga acattcaaac ataaatcgta
     6241 ctcgtgcaaa tgaggttatg gaaagccgtg aaaaagactt atttgaaggt gtgagacatt
     6301 acttagaaac tggagaaatt tctgaaaaag cattttatgc aggttcacat ggagattgga
     6361 ttagtgattt ggcagtttca attaaaaatg atacacgtag tcgtttttta gttataactg
     6421 aaaatcgtgg agctatacca aacatgccat atgatgcaat ggtagaaatt ccagcatata
     6481 ttggtaaaga tggtccagaa gttattgctc gaggtccaat acctctattt caacaaggat
     6541 taatgatgca acaacttaac agtgaaaaat tgatagtaga agcttatata gaaaatagtt
     6601 atgaaaaagc tttacaagca ttcacattaa ataaaactat tccaagtatg aatgttgcta
     6661 aagaaatttt ggatgaaatg atagaagtta ataaggaata ctggccagaa ttaagatagt
     6721 aggataaagg aaagggtttt tatttaataa ttagatatgt tttcagagtc ttttatcaaa
     6781 atgtattcac aaacatatgg caatatatta aaataaagag tgatattttt agaagaatgg
     6841 ggtgatgcaa tgaacattat aaataaaatt ttttgggaag aagataggat tggcatgatt
     6901 acaaataatt tagaggcaga tacacatagc cactgtatgt tacaactgtt cttagggata
     6961 gaagacagca ttgaaataac agtcaatgaa aaattagtta aatgtaattg tatcatagta
     7021 gataaaaata tctctcattc attttcagca agaaaaaaag tttattactc tgctattatt
     7081 gaacctacat ctatatatgc agaacagctt acctcaaaaa tgaatgattt tggctactgg
     7141 atatgtgaca atgatggatt agagaaatta cgtcaacaag gaactttttt gatagataac
     7201 tctagtaaag aacaatattt aagatttatg gaaatgctta ataactattt gaatatacct
     7261 ataactctca aacattatga tgacagaatt actgaattgc tcaatttatt acgtacttgt
     7321 aattgtgata atcatactat ttcaagtttt gcagataaag tgtccctatc agcaagtaga
     7381 ttatcacatc tgtttaaaga acaaataggg atacctttaa aaagctatat cttatttcat
     7441 caaatggagt gtgcttttag agaattactt tctgggaaaa atgttaccga agcttctatg
     7501 actgctggat tcgatactcc atcacacttt gcagggacag ttaagcgaat gatgggaatg
     7561 cctgtttctt tatctttgaa agatagcgaa tttttgaaag tatattaaat attctcgtta
     7621 tataatgaag ttgtactaag aaaggggctt tgttatgaat aaagaatatt tagaggaaac
     7681 aaaaatgtta aattataatg aaccacaact taagcttttg gtatcatcaa agaattggtt
     7741 ggaattagat gattttcata aaataaaatc tatttatgag tttgtacaga atgatatttt
     7801 atttggatat aatgcttctg atatgttaag cgcaacacag gtattaaatg atggtatggg
     7861 acaatgcaat acaaaagcta ctcttttaat ggcattatta cgtgcagtga atatcccttg
     7921 taggcttcat gcttttgatg taacaaaaga ttttcaacga ggagctacat ccaaattgat
     7981 ttcactatta gcaccaaaat atattcttca tacttgggta gaggtatttt atcaagatag
     8041 gtggattgct cttgagggtg tcataactga taaaaaatat ttagaagcaa ttcagaagaa
     8101 attttttaat catggtggaa catttaaaaa gtatgcaata gctacaaatg atttgaaaaa
     8161 tacatctatt gattgggatg gtaaagatac ttttatccaa aaagaagcta tcgtgtatga
     8221 ttatggtatt tttccatcac cagacgtatt tttttctact cattcacaac atatgtcaaa
     8281 attaaaaaat ttcatatatg tacatcttat cagaaaaata atgacaaaaa atgtatgtaa
     8341 ggctagaaac aattatattg ataaaaacga atgagtgttt aagaggtaac tatataggaa
     8401 atatatagaa taattaaaac tgtactggtt aaaatgaata aaaaggagtt atcacagagc
     8461 aaacttaaat tgttcatagg ataactcctt ttctgtatta acttctaata atttgatgtc
     8521 tttttataaa acatatagat acaattaaac cagtaaatgc aagagtagct gctattgaaa
     8581 atgcaacatc tattccatga atgttagaca gcatgtttga actcatatga gctttcttag
     8641 tagcacttgt cattatagta ataagtatag cagaacctat agctccagat atctgacgga
     8701 gtgtgttatt aattgctgtg gcatgagata taagttctct atctaatgtt ttaattcccc
     8761 atgtagttaa tggcattaaa accattgaaa taccaatcat acgaatacaa tacatcaatg
     8821 acacatgaat taaagatgta tctctgccta aaaaagcgaa ggataaagtt cctaaaaaca
     8881 aacatcctgt tcctaatatt gatagagcat gaggtccata tttatctaaa tgacgtcctg
     8941 caattggatt gagtacaacc atcaatatag caccaggtaa cattaatgaa cctgaagata
     9001 atgcagaaaa tcctcttaca gattgaacgt acagaggaat cattaatgta gcagacatca
     9061 ttgaggcata tacaatcata attaatattg tagaaataag aaatggttta tttttaaaaa
     9121 ccctcaattc taaaaaaggt tctttagatt ttaattgacg caatgtaaat aaaattaatg
     9181 acataattcc tattaatatt ggtaaatatg tggcaatatt agtccaccca taattacctt
     9241 gattagtaaa tccaattaat aaaccaccaa aacctagaga tgatagtact attgaaggta
     9301 tatctaattt tagtttttga gtttccccaa catttcttaa tagaataaat gatagtataa
     9361 catctaatat agcaataggg gaaagaaaca gaaataaact atgccaacca aatgaatcta
     9421 ctaaccatcc tgataaagtt ggacctattg ctggagcaaa tcctactgta ataccaacta
     9481 aactcatagc tgcaccacgt ttttcctcag ggcataaata gagtataata acttgtaaaa
     9541 gtggcattag agaaccagaa cccattgcct gcaacacacg agcaatcaac atagttgaaa
     9601 aattattaga aaaaagtgca ataatacatc ctatagaaaa gatagacatt gatgtaataa
     9661 ataactttct agtactaaat cgatttatta aataagctgt tgtgggtaca ataatacctg
     9721 caataagtag ataaattgtt gttaaccact gaccaatatc agcagtaatt ttaaaatctg
     9781 acattatatt aggtaatgct gttgacaata gtgtttgact gagagaactt ataaaagcac
     9841 cagtcattac aattgctatt attaaatttc cttttttatt gttactttgg gtttccataa
     9901 tttataacgt ctcctttttt aattgatttt taatgctgtt caataaaagt acttttggtc
     9961 ttaaagtgtc ttctttttct aaatatgcaa tcattgcatc cataattaaa gaatcatatt
    10021 gttgattttt tccttcgacg tataattttt ttattgcttc tagtaattta ggattttcaa
    10081 ggagagaagt atcagttttg attagttctt ctatatcttg tatcgtttta aaaaatagcg
    10141 atttattatg aagggaaaag taaacttcat cttcttgttt atgtaaaaac attgattttt
    10201 caattaaagg atttagatta tccatatttt ctacaactaa atctagaatt tgtatagtaa
    10261 atttggcaat aaagtataca tttatttctc ttttttgtac atgaataatg tatataaact
    10321 gatgaattag agcatccaat aagaaaacta aatcaaatat aattttttct attttatcac
    10381 catagttatc aaataagctt agtttgtatt cattcaataa gttagctctt gattttgttt
    10441 gcaatttact tgtagcctta cgttgctctt cagaaaagtt ttctcttata taggtattta
    10501 tggtagttct agaaaaaata tttttccata cagcaattat ctttctgttt aacttttctg
    10561 atgatggaat gtcattattt tcatttatgg aatgtataat ttctaaaaac ttttcatcat
    10621 aataattgat tatttcgcag ataaaacttt ccttagtttc aaaatgcttg taaaaagtag
    10681 ctttagacat tttacactct tttacaacat ccgatattga agtattttga aaaccttttt
    10741 ggtcaaaaat tttttgtgca atagttataa tgagcctttg tttttctgtc actttattat
    10801 ctcctttcac gcaaaaacgt ttagtactat atagtaccta caagttttga taaatatatt
    10861 atacatcatt tggaaaaaaa atcaatatat agactgtaat atgaaatttt tgaaaaattt
    10921 gttgttattt ttagtcgtat aaaaaagaaa cttaagagtt tcttaagaat tgtttaaaga
    10981 agttgcaata aattagtggt atgattatag tatgaaagag aatgtcttga aatttttctg
    11041 tatcaaattc caattatgga ggtgtagtat atgaaaaaga aaatttgtgt agcaataatt
    11101 tttgctctat ttatagtttt tgtagaaatg tttattgata gctctagcga aggaagtaaa
    11161 attatgacag ctgaattaag taaatacagg aaaggcgtaa atgtattatc aataaacaat
    11221 attatagtag cgaataaaaa atatagtttg cccaaagatt attctcctca ggaaagcagt
    11281 gaagcgagag atgcttttta taaaatgaat aaagatgctc aaaagtcagg attaaattta
    11341 aaagctttta gtacatatag aagttatgaa tatcaagata gattatttaa gtcatatgta
    11401 aaagaacatg gagaaaaaga agcaaataga ttttcagcta aacctggaga gagtgagcat
    11461 caaactggac ttgcttttga tattggagga gatgaccagt cttgttgggc aaataaaaag
    11521 ttcaacaata caaaagaagc taagtggttg tatgaaaatg catataaata tggttttata
    11581 cttagatatc cagaaggtaa agagaatata acaggatata tgtatgaatc ttggcactat
    11641 agatatgttg gaactgaaca tagcaaaaac tttgcaatga ataatttgac tttagaagaa
    11701 tatttacata taaattaaga gatggtttgt taaaattagg ttaaaataaa aaataaccta
    11761 gactattagc tgtgtaaagt gatataatgt aaatacaatt taagataact actcgaaggg
    11821 gagtagctta cacgaaagtg gtaattaatc gtcaagacag tagaaatact cggttaatta
    11881 gcaagggatt ccttgatagc aagaccttca aaataagcat taggtatgtt tgttttgggg
    11941 tcttttttta ttcaaggaat ttttatttaa aaaaatttaa atttcaagta tacataagag
    12001 cccccaaaca taaaataaat ttattgaaga ggagggcaac aaatgtctta caaaagtact
    12061 tatcaagaag taattaaaaa tttagactca agtttatctg gtttgagtaa agaaagagca
    12121 gaagagttgt tagaaaaaaa tggagctaat gaattaaaag aagcagataa ggttcctact
    12181 tataagcttt tcctagaaag ttttaaagac ccattagtaa taattctatt gatagctgct
    12241 ttagtacaaa tatttttagg tgaaacagtg gagtctataa taatttttgc agttattatt
    12301 ataaactctg ttctgggtgt tgtacaaaca aaaaaagcag aaagttcatt agaaagtttg
    12361 aaaaatttat ctgcaccaaa cgcaaaggta ataagagaca ataaaaaaat gacaattcca
    12421 gcaaaaaatc tagtagttgg agatattgta ttcttagaag ctggcgatta cataccagca
    12481 gatggaaggc ttatagaagc acaatctctc aaggttgtag aagggatgtt aactggagaa
    12541 tcagaaccag ttttaaagca tatagaaaaa ataagtgaag atgtaagttt aggtgaccag
    12601 aaaaatatgg tatttagtgg tgctgtagtt gtttatggta gaggttcttt tgtagtgact
    12661 ggaacaggta tgaatactga gatgggtaaa atagctgggc ttttagaaag tgctgaaaat
    12721 aaacttacac cattgcaaca aaaaatagag gattttagta aaaaacttgg tgtaggtatt
    12781 gtaatacttg ctcttttaat atttataatt caagtagcaa gaaattactt tatggctgga
    12841 aatggagaaa tgacacaaat tattttagat tcattcatgt tctctgtagc agtagcagta
    12901 gcagctatac cagaagctct atcatcaata gtcactatag tattggctgt tggaacaaat
    12961 agtatggcaa aaaaacaagc cataataaga aaattaccag cagtagaaac tttaggttca
    13021 acaagtgtaa tatgtactga taaaacaggt actcttacac aaaataaaat gacagtagtt
    13081 gattattata tgtatggaac taataatgat gagttagata aagaaaaagt aaactattct
    13141 tatcatgcta aattattgac tatggtttct acattgtgta atgactctca tataactagt
    13201 gatggtaaag aagttggtga cccaacagaa gtggctttaa taaattattc aagtaaaaat
    13261 gatttagatt atgataaatt aagaagtaag tatatcagaa taaatgaaat accatttgac
    13321 tcagacagaa agcttatgtc tactgtaaat agtatatatg gagattatat aatgtttaca
    13381 aaaggtgctc cagatattgt gtttagtaga tgtaaatatg ctttgaaaaa tggttcaaaa
    13441 gtagatataa cagatgagat aatagaagaa tataaacata aaaatgaaga gttctcaaat
    13501 agagcactta gagtattagc ttttgcaata aaagatgtcc cagaagaaga ttttatacct
    13561 tcaattgatg acgaacatga catgacattg gttggaataa tggcaatgat agacccacca
    13621 agagaagaag taatggatgc agtaaaagag gcaaagagtg ctggtataaa aactgttatg
    13681 ataactggtg accataaaac aacagcagca gcaattgcaa aagagattgg tataatggaa
    13741 gaaggagact tggctcttac tggaaaagag cttgatgctt tgagtgaaga acaactatat
    13801 gaaaaactag aaaatataag tgtatatgcc agagtctcgc cagaaaataa aattcgtata
    13861 gtaaaagcat ggcaacataa gaataatata acagcaatga ctggtgatgg tgttaatgat
    13921 gcacctgcat taaaacaagc agatatagga atcggtatgg gaagtggtac tgatgtagca
    13981 aaagatgctt ctgctatggt attggttgat gataactttg caagtatagt aaatgctgtt
    14041 gaagttggta gaacagtata cagtaatata ataaaatcaa ttacttatct atttgcagga
    14101 aacttaggag ctatagtatc catagtattt gcagtgtttg caggttggtc aaatccattt
    14161 acagctttac aacttttgtt tataaactta gtaaatgact ctttaccagc aattgcatta
    14221 gggtttgaaa agtcagaaaa aaatataatg aataaacctc ctagacaccc ggatgaaagt
    14281 atactagcag gaggaacatt gaaggcagtt gctataagag gttgtataat aggtgtagtt
    14341 acaataattg cacaatatat aggattacaa gtatctccag aacttggagt agcaatggca
    14401 ttttcaacat tgatattagc tagaataatg caaacattac ctgcacgttc taataatcaa
    14461 acagtaataa aattaggttt tttcagcaat aaatacgtta taggagcagt tttagtctgt
    14521 cttgggcttt actctttaac actaatacca gtgctaagag gaatattctc tataccaaca
    14581 acgtttggat tgtaccagtt ggggatttgc ctagggttag cattaatttc atcaatatta
    14641 atggaactta acaaattaat aaatagaagt gaagtaaaat aatcaaaaaa ttaaagggca
    14701 tattgatttt aacaatatgc cttttataaa tagtctaatt tattctatat tatcgattat
    14761 ttggaatgct tttataactt caggtaattc tttttcttca ggcattctaa atcttatgaa
    14821 atttttatca agtccagtaa aatcacttcc agatataact cttattttta atttagcaaa
    14881 ctcttgttgt aaatctatgt ccttgttttt atgttcaact gtcataattg atacagtatc
    14941 actagtttca gctatattaa ggtttttcca tggaattaat aattgatttt ttatatcttt
    15001 tgttttttct tttaattctt ctataaattg aacatcatct aataaatttg ctgcaatact
    15061 acgagatatt tcagacactt cgtaaggagt agaaatcttt ttaatagggc taacaagttg
    15121 ttctggaaga actgcatatc ctgctcttag tcctgctaaa ccaaatcctt ttgaaaatgt
    15181 ttttaaagca attacattat cataattatt tagaagcttg acagctgaat tttcttttgg
    15241 catgtattca ccataagctt catctaccat aactgctata tcatatttag ctgcttcctt
    15301 tactatattt tcaattgatg ataaaggtat aatttgtcca gtaggattgt ttggattgtc
    15361 tatgtaaatt actttatatt ctggatttat tttttcaata aattcttttt cattaaactt
    15421 gaaattatcc tctttcttta aaagaacata atcataagtt gcaccatgca tttttatatc
    15481 tgtctcatat tctgagaact gaggtgaata tcctaaaact ttatctccct tttcaataaa
    15541 tagtctattt aaaaggtaaa taacatgtat agaaccatct gccaaacaaa ttctgtcagt
    15601 atctaatgct ataaaatctt tccaataatc aactatatta tcttttaaag caattgaatg
    15661 aggatatcct cttatcatat caaattttaa agcattaaaa gcttctacag ctttgtttga
    15721 gtaagacacg gtatttatac cttcaccaca gtctatctcg aatacatctt ctgaatgtcc
    15781 agattctatt gcgtaacttt ttttcaactg catttcaata gctttgttaa tttttagttt
    15841 catactttat cactccttat ttagtcaata ataaatgtta taaaagttgc tagatttaaa
    15901 atattatgaa aatatataat agtaaaaaat gaatataaat attataatag ctcactttat
    15961 ttaatatgat tatatatcat atattgtaaa atttctagca ttataagact ataaattgaa
    16021 aatattcaga aaaatactac aagaaacgat ttattttgct cttaaaataa gttaaatgaa
    16081 tgttattaat tttgtaaatt gaaatagttt aagtataaag ttatgatata atctaataag
    16141 ggtaattctt tagattatat taaggaaata taggtataca ataaaaagtt atggagttgg
    16201 attatgggat ataaattaat tgtaacagat atggatggaa ctttattagg caataatcat
    16261 aaagttacag atgaaaataa aactgcacta caaaaagtta taaaaagcgg tattaatgta
    16321 actttagcaa caggaagagc cttcgattca gctaaatgca atgtagattt tcttaaagag
    16381 gacatgccaa taatagcttg taatggttca ttaataagag agcaaaatgg aaacataata
    16441 tattcaaata agattgatac aagaacatgt ttaaacattt tagatgtatt agataaatac
    16501 gatatttact accaatgtaa tagtatagat tctatgcttt cgaaaaaaat agaaggacgt
    16561 gaagatagat taagtgtatt tttaggctct gaaacagaag taattgtaaa agatgatttg
    16621 agagaagaaa tctttaaaaa agatatacta aaatttgtaa tcatagaaga aaaaaatcca
    16681 tctattttgg atgaaataag aaaagaattg agaaaagttc aaggaataaa gataacttct
    16741 tcatggccaa acaatattga agtcatgaat gaaggtgttg ataaaggtaa tgcagttaaa
    16801 attttagctg aaaaaatgaa tatagacaga gaagatataa ttgcatttgg agacaattac
    16861 aatgacatag aaatgataaa gtttgctggt cttggggtag ctatgggaaa tgcagaagaa
    16921 ttgattaaac aagaagctga ttatgttaca gatacaaatc aagacagtgg agttgcaaag
    16981 gcaatttata aattcctaga gttaaaagaa tcacttagct cttaaaacag tatataatag
    17041 gaaaaaatag gttgactatt ttgtagatag taattataat acaaatagat aaaacataaa
    17101 aatttagatt cagtgaaaaa gagagtagat atagagaaaa tctcagcgaa ttgggggatg
    17161 gtgtgagcct aatgataaca ctttatattg aaaagaactt tggagaatac tacttgaaat
    17221 tcagtatggt agtaggtaat ctcgccttaa gagaatagag tgggtaaaca ttgtttacca
    17281 actagagtgg taacacggaa tatttcgtct cttggtcttt ttgaccaaga gattttttag
    17341 tttcattgag ttacaagact tataattaaa atacaaaaaa ttaaaatatg aggtgaaagt
    17401 tatgaaatta tggggcggac gctttagaaa agcggaaaat caacttatgg aagaatttaa
    17461 taaatctttt ggctatgatt gtgtactgta caaaaaagat atcgaaggaa gtgtagcaca
    17521 tgttcatatg caagtaaaat gtggactttt aacagaagaa gaaggaaagt ctataacaga
    17581 aggtttaaaa gggatacttg aagatgtaga aaatggaaag ttagtattgg atggagagta
    17641 tgaagatata catagtttta ctgagataaa tttaattcaa agaattggag atgttggcaa
    17701 gaagcttcat acagcacgta gtagaaatga ccaagttgca gttgatatga gattgtatgc
    17761 taaagaaaaa gcaaatgatt tggtgggttt aatatctgaa tttaaagcta ctataaaaga
    17821 tgttgctgac aaaaacccag tgatgatgcc gggctatact catcttcaaa gggcacaagt
    17881 agtgacattt aagcatcact taatggcata ttatagtatg tttgatagag atgagaaaag
    17941 aataaaaaat gctatagaaa tattagatga atcaccttta ggctgtggag ctttagcagg
    18001 aactacgcat gatatagata gaagtataac ttgtgaacaa ttaggattta aaaaagtagt
    18061 agataatttt atggatggag taagtgatag agattattta ctagagctaa tgagtgattt
    18121 ttcaattata atgatgcact taagtagatt aagtgaagag ttgatacttt ggagttctca
    18181 agaatttgga tttgttgaga tagatgattt atatacaact ggaagtagta taatgcctca
    18241 gaagaaaaat ccagatggtg cagagcttat aagaggtaag actggtagag tatatggaaa
    18301 cttatttggt ctttttacag taatgaaagg aataccatta gcatataata aagacatgca
    18361 agaagataaa gaaggttttt tcgatagtgt acatactctt gaaatgtgta tacaaataat
    18421 ggatagaatg atagccacac taaaggtaaa tgaggataaa atgaaacaag cagtaaaaaa
    18481 tggtttttta aatgcaacag aagttgcgga ttatttggtt aaaaataatg ttgcttttag
    18541 agatgcacat ggtatagtag gaagtatcgt tatatattgt gaagataata aaaaagcaat
    18601 agaagattta acattagaag aattacaaaa gttttcagat gcttttaagg aagatattta
    18661 tgattttatc gactatgaga gtatattaaa taagggaata aaaaagaatt taaaataatg
    18721 tatttatttt taaaagctgt aaatttgaat gttatcattt aaatttacag ctttttattg
    18781 tgtaagattt tattatataa aagtaagaaa cttaaaagga gtagtattta aaataaatac
    18841 aaataaaaaa ttattattaa aatgataatt aatatcaata agtattgaca aaattatttc
    18901 taaatactat tatatataat ataaataata atgaaaatca atatcaaata aatgggagga
    18961 aatctaatga acttattagt gattggtggt catgaaagaa tggaaaaaga ttatatggtt
    19021 atggccaaga aaaaaggtta taagacaaag gtatatacta ccatgtcatc taaattaaat
    19081 aattctatag gaagaccaga tgcagttgtc atacttacat caactgtttc acataagatg
    19141 tctagaacgg ttgagtctca agctaaaaaa caagatatat taatcgtaag gcataaaaat
    19201 agtagtaagg tagcatttaa tgaatgtcta gatatggttg atgaatgtct gggaaattgt
    19261 tcaaaatgta agcttaaaga caaacaaaaa caaaaaaata tataggtata aaaaatacat
    19321 aaacataaaa ttaaaagtac tgagataata gttataatta ttatctcagt acttttagat
    19381 ttataaggat aagtatagta taaatttttt attataattt ccaactactt gttacttttt
    19441 catatttttt ttacttctag ctttttttct ttttttcttc tttatatttg aagctattat
    19501 aaataaaact aaaagcacta gaatacctgc aataatctta acaaaagtca ttaaaacatt
    19561 attttcagtc aaaaatggaa gactgctatc aagattattt tttgcaatta agtctatact
    19621 gttttctagt ttaccattat tatatacatt taaagtacca accttagccc ctttttttat
    19681 aggcaaatca attttattta actctgtttt ggtatcataa ttgccttgag attcatcttt
    19741 ttctaagaat atcctataac tattttttgg ttcataaatc aattcatgtt gttttgtaaa
    19801 caatactttt ttagtatctg tgtatttttc tttatcaaca atagtagcac acttaaagtt
    19861 atcaaatcca taatcaatca gaatacgtga gtctaaatat aaatcagcat tagttgaatt
    19921 aaatacagca acgataactc ttctgccatc ttttacagca ctcgataaaa gacatttacc
    19981 agcagcatca gtatatccag tttttatacc atcaactata tcatatttta tatttatagg
    20041 ttgacctttg tatgtaatct tatcatgtga tgtcaaaaac ttattagtgt ttacaaagta
    20101 tctctcataa ggatatgctt ttgtagcttc aaatttaagt gacttagttt ttacaatttg
    20161 tctaaaagta tcattattca tagcttctct agctatgaga gccatatcat gagctgttgt
    20221 gtagtgattt gggtctggta atccatgagg attattaaaa tgagtattag tagctccaat
    20281 tttttttgcc tcagagttca taaggtcaat aaatttttgt acattgccac caccaacata
    20341 tctagctaat acaaaagcag catcattagc agaatgaacc aaaagagcat ctagtaactg
    20401 ctttacagta aaagattccc cattttttag atacatacta gaaccatcta tttgaggtaa
    20461 atctttaatt tctattactt gatttaaatc tttaacctcc tttagaacta aacaagctgt
    20521 ccatacttta gttgttgaag ctggataaag cttagaattt ccatccttat tatatagtat
    20581 tttgccagac tcatagtcca ttaaaactgc atgtttagca actatagctg gttcattatc
    20641 tgcaaaagag atattagagc ttcctaaaaa tgaagtaaat atcaataaac agattaataa
    20701 aagtgataaa tttcttttca ttgtgagcct ccaaatttta aattcatctt ttagtatagc
    20761 atatatttgt caataattaa aagaaaggag tgattaatat atgaaaaaaa aggtaaaatt
    20821 tatttcgttt tttgttttat ttattttaat aatatcatca ataatcgttt ataaatcgct
    20881 tattttcaaa gatgatattt atgttgtaag taaaaaaaat ttacctaatg aaaaaacaca
    20941 tgttgtagag aatgaaatta aaggtgaaga taataatcac agtaaagtaa ctaaagacat
    21001 agataaaaat tttcaaaata ataaaataac tatatacata agtggagcag taaatagacc
    21061 tggaattgta actatagagt cagacaagag actttatgat gctgtagaat tactaggagg
    21121 aacgacaaaa gaagcagatt taaatggagt caatctatca gtaagattag aagatgaaca
    21181 acattacata atacctaaaa taggtgaagc aacatctgtt acaagtaatg atgattccaa
    21241 taaaccaaat cagaagaatg agtcaagagg tgagcctaaa aataaatcca ataaaaatat
    21301 atctaacgaa tcaaaagtaa atataaatgt agcgactata gaagagttgg attctctacc
    21361 tggggttgga gaagctacag ccaataaaat tttacaacac agagaagaaa atggacagtt
    21421 tagttcaatt gaggaaataa aaaatgtaaa tggtattgga gataaaaagt atgaaaatat
    21481 aaaagattta atttgtgtag actaaaatat aatacaaaac ttacatatag atatgttatt
    21541 ataaatatgt atgtatatgt gtgtacatgt ggaaatttag taaaggaggc acttttatga
    21601 aaaaagaaca aaaaaaactt acagagatga caacagcagg tggttgagcc gctaaaatag
    21661 ggccagaggt tctggcatca gttttatctc aattgcctaa aaatgataat atagaaaatt
    21721 tattagtagg actagataca gcagatgatg ctgcagttta taaattaaat gatgatatgg
    21781 cacttatcca aactttagac ttctttacac caatggtaga tgacccatac gtatttggtc
    21841 aaattgctgc atcaaattca ctatcagatg tatatgctat gggaggcaaa cctttggttg
    21901 ctatgaatat tgtttgtttt ccatcatgcc atgatatgga tgtacttgca gaaatattaa
    21961 aaggtgggtt tgataaagtg aaagaaagtg gagcactttt ggttggaggg catactgtag
    22021 atgacaaaga gcctaaatat ggtctttcag tttctgggat tgtaagtcca aataaagttt
    22081 tatcaaatgc tactgcaaaa cctggtgata agttaattat aacaaaacca attggagtgg
    22141 gtgtattaaa tacagccatg aaagaaggta tggtagaaca acatatagca gataaagtaa
    22201 tagaaataat gatacattta aataaatatg cagcaatgag ttttgagaaa tttgatgtaa
    22261 attcggttac agatataact ggttttggtc tacttggtca tactttagaa atggccaaag
    22321 cttctgaagt aagtattgaa attgaatcaa aacatgtacc aattattgaa ggtgcaatag
    22381 aaatggcaca aatgggaatt atacctgctg gtatgtataa aaatatgcat tatgtatcta
    22441 aagatgtaga agttgtagga aatatagaag ttgcagttca agatatttta tacgacccac
    22501 aaacatcagg agggttgtta atttcagtaa aagaagaact tgcagaggag cttgtaaaag
    22561 atatgaaatt aaatggagca atagaagcta aaattatagg ttctgtagtt ccaaaagggg
    22621 aaaaatatat tacagtttta taaaatatta tagagaaaga gtgattgttt tgagcaaaag
    22681 agaattgttt gcaatgttgc cttcagttga tgaagtatta agtgataata gaattgtaga
    22741 aataataaat gaatatccta gaagtttagt tttagaatct gtgagagaag taatagattt
    22801 aaaaagacaa tttatattaa gattaaaaga agatgcatca aactctgtaa caatagagtt
    22861 tggtgaaata atagaatctg caatagagag agttaagtta aattactcat tatctttaaa
    22921 aaaagttata aatgcaactg gtacagttat tcatacaaat ttaggaaggt ctttacttag
    22981 tgaagatata aaagatgaat tatggtgtgc tgcttcaagg tattcaaact tagaatacga
    23041 tttggataat ggagaaagag gttcaagata ctctcattta actagtacaa taaaaagact
    23101 tactggagca gaagatgtac tagttgtaaa taataatgca gcggcagtac ttcttgtact
    23161 tagcacaatg gctaaaggag gagaagcaat tgtatctcgt ggagaattag ttgaagtagg
    23221 tggttcattt aggattccaa gtataatggc tcttagtggt gcagaattag ttgaagttgg
    23281 ttcaactaat aaaactcatt tgaaagatta caaggaggcc attacagaag atacaaatgt
    23341 attgatgaag gttcatacaa gtaattatag aataatggga ttcacagaga gtgtttctat
    23401 agaagaattg gttaatttag gtaaaaaata taaactacct gtaatagaag acttaggaag
    23461 tggagttttt atagatttat caaaatatgg tttatcttat gaaccaactg tattggattc
    23521 tattcgacaa ggtgcagatg tagtaacttt tagtggagat aagatgcttg gaggtccaca
    23581 agcaggtatt atagttggga aaaaagagta tatagaaaaa atgaagaaaa atcaacttac
    23641 tagagcactt agagttgata agcttacaat atgtgctctt gaagcaacat tgagaatgta
    23701 tttagatgag acaaaagcta tagaaaatat acctacttta aaaatgttga catataaaat
    23761 tgaagaatta gaagtaaaag caaataaact atttgaaaaa ataactgctt taaacttaaa
    23821 tgcaaacata aatatagaag atggattttc acaggtggga ggagggtcta tgcctcttga
    23881 aactataagt acaaaggtaa tatcaataac tcctgaacat atgaatgttt catctttaga
    23941 aaaaaaacta agacttagtg aagcacatat aatagccaga gtgtatgata ataaatatgt
    24001 actagatgta agaactatat ttgatgatga atttgatgta atagttgagg aattgagaaa
    24061 agcatttaat taaataaatt tgagttaata aatattaaat ttagaagtat aagtatgaag
    24121 ttttgattaa aaaatagcta cacacacttg tgactttagt catagcaggt ttagaggggg
    24181 caaaaaatga aaaatgtagt aattggaaca gcaggtcata ttgaccacgg aaaaactact
    24241 cttataaaag cattgactgg aagagagact gatacgttag atgaagaaaa aaagagaggt
    24301 atttcaataa acttaggatt tacttatttt gatttaccaa acaatagaag agctggaatt
    24361 gttgatgtcc caggacacga aaaatttata aaaaacatgt tagctggagc atcaggaatt
    24421 gatatagttt tacttgttgt agcagcagat gaaggggtta tgccacagac agttgagcat
    24481 ttagatattc taagcttttt aaatatcaaa aatggaatag tggttctaac taagtctgat
    24541 actgtagatg atgaatttag agagctagta aaagaagata tttgtgaaaa aataaaagga
    24601 acttttcttg aagatgctga aataattgaa gttgattcta tatctaaaag aggtatagac
    24661 actcttattg aaaaaataga taaaatgagt gatgatatag aagataaaaa tgaaaactct
    24721 ccagcaagac ttaatataga cagagtattt tcaataaaag ggtttggtac tgtaataaca
    24781 ggcactttaa tagaaggaaa gataagtata gaagatgatt tggttatata tccacaggta
    24841 ttaaaaacaa aaataagaag tatacaagtt catggagaaa ataaagatac agcttatgca
    24901 ggtcaaagaa cagcaatcaa tatatcaaat gtaaaagtgg atgatataaa aagaggagat
    24961 gttttagctg caccaaactc attggaggaa tcaatgatgt tagatgtaaa attatcattg
    25021 gtaaatcatt tagataaagg attaaagcac tgggatagat taagattgta tcatggaact
    25081 agagagatac tttgtagagc agttccacta gataaagaat taattgaaag tggagaaagt
    25141 ggctatgttc agttgagact tgaagaaagc atagtatcta agaaaggtga tacgtttgta
    25201 gtgagacgtt attctccaat ggaaactata ggtggaggtg taataattga cccatctcct
    25261 aaaaaacata agaaatttga tgaaaaagtt atagaagcat taaaaataaa agaaaaaggc
    25321 gaactaaaag atataataga agagtactta aaaaggaatt taaaaaatta tccaaatata
    25381 aaagaaataa tgagctatag tggtgctcat gaagaagatg taaaaagagc gcttgaaaca
    25441 cttatatcag aagataaagt atttattata ggaaatatgt atatgcatat aaatcaatac
    25501 aataaattaa aagaaaatac aataaagctt ttaagtgagt accataagaa gtataggctt
    25561 agaaaaggga tactaaaaga agaagtaagg tctaagatag agagtaattt taagactagg
    25621 gagatggata ttttattaga aaaattgtct acagaaaatg ctataaaaat agaaaataat
    25681 atagtatctc tattagattt tgaggttatt ttaaatgata agcaaaaaga aatagcaaaa
    25741 aaaatagaaa aaagattaaa atcatgtgga gtttcttcta tactgacaat agatgaagtt
    25801 tctgaaggaa atcataacta tgctgaagta ttggagtcaa tgataggtaa taaagtagag
    25861 aaattagatg atttgtatat aatggacaaa gatatttatg aaaatgcaaa gaacattttg
    25921 ataaattata taaaagaaaa taaagaaata acattaggag aatacagaga tttgattgat
    25981 tctagcagaa agaattgcat gattatattg gaaaacttcg atagaaacaa aataactaaa
    26041 agagtagaaa acaaaagaat tttgttttag tagaaaatgt ctgtttttgc tttaaggtat
    26101 aaagaaggaa ttataatgtc gagtatcgta taattaatta gtataaaata ttaattaaga
    26161 ggaagtgtaa agtattgaag aatataatac ctgatgtaaa accagatata aaagaaagag
    26221 aagacatgag ccttttattt ataataatgg gctcagtact tttattattt attataaaag
    26281 atatagagaa atatgaaact ttattacatg ccatatatac gatattttta gtagctacag
    26341 gagtaattat atttaatact acaaaattta aagtagattc attttctaca tttttaggat
    26401 taatttttat agctactgga gtattggagt gtatatattt atttaattcc ttggggatta
    26461 aaactaagtc tataatggag attaacatca caatatcagc aataacagat ttatttccta
    26521 tactgggtgt atacttaagt tttaaatttg ttaaggataa caaacaaatt tattcaagtg
    26581 taattttatt tatagcaact gctacattaa caataacatc tttattcata atatcaggaa
    26641 tttgtgacta tctgggaaat ggtaaatcac aatatgcttt aggagttata atatctatat
    26701 tcataataat tatttctatc gttagtggaa ttgaattaaa ttcaagttca aataaaggta
    26761 aatgggaata tgatgaaaaa aagattttta acagaataat agtaataata atcttatcaa
    26821 gagttccaaa tctattgcat atagttattg ataatagaaa tgtagagagg atattatcac
    26881 aaataataat aaatgtagcc ttatattatt tatataatta catagtatct aagaatatta
    26941 agaaaacagc actggaattg catgatacga atgaagaatt aacaaaaaaa acggaaagtt
    27001 taaaagaaaa gaataaaaaa ttaatctatg agacagaaaa gatagaggaa cttaagggaa
    27061 tacttatgaa aagagaatct aggctacaat ctacattaga tgtagcagta aatagcatag
    27121 tagtatttag caaagatgga gaaattacat atgcaaataa gagctttaga aatacttttg
    27181 gagactataa agaacaagat aaattgactg ataaaataaa aaacttcaat gatttgatag
    27241 aaagcattca ttatgtgttt gttcatgaga agaatgcaga gaaacttatt agtacaagcg
    27301 ataataaagt ttatcaagct atatttgcac ctttaattat tgcttcacaa acagaaggag
    27361 tattgtgtgt actagaagat aagactaaga aaaaggagta cgaacgcaaa cttattgaag
    27421 caaataagag aagtaaagat tttttagaaa gtattggaga tggcatagta gtgcttgaag
    27481 gtaataacaa aatatatgtt aataatgcat gtagggaaat ttttaaaaat aatttagaga
    27541 gtattgattt ttcattagta tgtaaaagtg aagaaagtat agaaaaaaga tatgtaatag
    27601 atggtgaagt aaagtatgta gagatgagtt tttctcagta tactaatgct ggtactaata
    27661 aaactattat tgtaatacgt gatacaacct ctcgaaaaat atctcaaata aaacttgagg
    27721 aaagtcaatc atcatattca agatttatag atatattacc tgatggcata tgcttattaa
    27781 aagaagattt aagtataaac tatgcaaata agtcgttttt agatatgcta gcttttacaa
    27841 acatagatga tataaaagat tctaatatta aactattaat gaatgcgaat actgaagaaa
    27901 aaatgaaatt tacggataat atggctaaag ttttaaatga aaatagaagt atacttttac
    27961 tggaatatga attgataaat agttatgatg atatagtaga agtagaatta agtgcattac
    28021 cttttgctat atacaatact agatacataa tgcttattat aaaagattta gtacataaga
    28081 aatattccga gcaagcagaa aaagaactgc ttgaaagatt taaaacagat aaaatcaaaa
    28141 cggagttttt tgcaaatatg tctcatgaac taaaaactcc actaaatgtt atatctagtt
    28201 caaatcaact ggtagattct ttctatagaa atgaaaagat aaaagattac aataacaata
    28261 ttaaatctca tgtggattta gttagacaaa gttcttatag gcttcaaaga ttaataaata
    28321 acattataga tttaacaaag atggagtctg ggttctatac actaaaactt gcgaaataca
    28381 atattgtatc tgtaatagaa gacttattta tgaatataga agagtatgca cttagaaaag
    28441 acataaaaat tctttttgat acagaccttg aagaaattaa tgtttatata gacaaggttg
    28501 aaattgagag aattatgttg aatctattgt caaactgtat taagtttact gataatggag
    28561 gctggatata tgtaagtatt cattataaaa tagataaagt aatcataagt gtaaaagaca
    28621 ctggagttgg aataccacaa gataaattag agttgatttt tgaagaattt tcacaagttg
    28681 ataagacttt atccagaaat actgaaggaa gtggaatagg gcttgctata gttaagaatc
    28741 tagtaagtct tcatggtgga gatatagaag tagtaagtga agtgaataaa ggcacagagt
    28801 tcttaatttc acttccaatg aagggattta gtagtgaaca ttacacagaa gataagagaa
    28861 tatataatat tcaagaaaaa ataaaaatag aattttctga tatatattat taaaaatgat
    28921 tatgagcaaa aataaaataa aaatactatt tgtttaaata tttaagttgt aagaaatagg
    28981 ctatcataag gtaaattatt atgatagcct atttttagct aggttttata tctatatgtg
    29041 agaacatata atctctaaat taccttcaaa actaaaatct ccaaagtcgt ttggtagtat
    29101 aaaatgcata ccctttttta aattgtattc tacagaattg tgtataagtt ttccatttcc
    29161 atccaacaca ctgtataaag aaaaaggtgt attgtgtgta aaattacatt ttccaaaaac
    29221 atctaattta taaacactaa aaaattcgtt tgacacaaat gttgtgcatt taaagtctga
    29281 tgttgatacg attttatagt cagtatcgaa atttatatga ggaacattcg taacatctat
    29341 tgatttttga acatgaagtt cacgcttatt acctgaattg tcagttctat cataatcata
    29401 gactctataa gtagtatctg agttttgttg agtttctaaa atcaatgttc ctttgcatat
    29461 agcatgtata gtgccacttg gaacatagaa aaagtctccc ttttttatat taacctttct
    29521 aagtagtttg tcccattcat tattatttac catatttaca aattctttat gagattttgc
    29581 attatgacct attataattt ctgcatcatc agaacaatct attacatacc aacactctgt
    29641 cttacctaac tctccatttt catttttttt agcatattca tcatttggat gaacttgaac
    29701 tgataaatta tcatttgcat ccaatatttt tgtaagtaaa ggaaacttat caccaggtgt
    29761 gtttccaaat aattctctat ttttattcca caattcagat agttttttac ctttatattt
    29821 tccattttct attaagcaat ctccattttt atgagagctt atagcccaac attcaccagt
    29881 tgttggcgaa tctatttcat aattaaattt atctttaagt gcagtaccac cccatattct
    29941 atccataaaa ataggtttta aaaataatgg ttccataatc ctgttcctcc tatgatatta
    30001 aatttatcct acaaataatt atatataaat agattataaa atcaatagta aaaaagtttt
    30061 gtcccttgaa atgacataaa gtataaggag ttattaaata agataaacta tgatatataa
    30121 tataaagttc taaattggag tgataattat ggagttgttt aaggcagaaa gcaaaaaata
    30181 taaaaaagaa tttttaaaat tattggagca atgggttagt ataccttcat tttatgatag
    30241 aaaaactgta tctaaagaca tgccttttgg aaaaggagta tatgatgcat taaactggtt
    30301 tgaaaatcta ggtagagaaa acaatttcaa agtaaaaaat atagataatc atgctgttca
    30361 aattgaatat ggaaatggta aagagtatgt agatatattt ggacattgtg atgttgtaaa
    30421 tccaggtgaa ggatgggata gtgaaccatt taaactaaat attataggtg ataaattagt
    30481 tgctagaggt gtatctgata ataaagggcc tatgatagtg aattttttag cactaaaaat
    30541 gataaaggat ttagatatta atttaaaaag aaaggttaga ttaatagctg ggggaaatga
    30601 agagagtgga tttaaatgta taaagcatta ttatagcaaa gagccatatg gtgtatgtgg
    30661 atttactcca gatgctaaat ttcctgtatt aaatggagaa aaaggtggag caataataaa
    30721 gttaatttca aatatagacg ataaatcttt atatataagt ggtggaatag agtttaatac
    30781 aataccagat aaagtttata taaagaatgt agaaaaattg ggtaaagata atatatgttt
    30841 tgatattaac aatatatcga taaattatga taatggcaat tatattgtgt atggaaaagg
    30901 tggtcattca tctaaacccg aaaaatcaat aaatcctata ttagctacaa ttaaactttt
    30961 gtcagaaaat attgatgaaa aatggacaaa agatttatat aagttgataa atcaagataa
    31021 tataaatgga aacttatttg gtttaaatat tgaggggaaa tgtggaattt tatcaatggt
    31081 gccaacaata ataaatatag tagatggaaa attagaagta gttttaagcg ttagatatcc
    31141 agaaatatta accattgaag atataataaa aaaatttaat ttgtatatgg agcaaaataa
    31201 tattaataaa tttgagctta ttggggaaaa tttaaagcaa gcaaattata tagatagaaa
    31261 ttcaaaactt gtaagaagtt tgcatgatat atatataaag tattctggag atttaaaaaa
    31321 tgatgttaga gtaactagtg caggaagtta tgcttctgaa atgaataatt cagtaatatt
    31381 tggttgtgaa tttcctgatg gtagctttgg caatgtccat agtgcgaatg aatttgctag
    31441 cttagataga tttataactg caataggtat atatgcagag gcaataatta cattatgtaa
    31501 taaaatataa atacaaaatg tcccttatat agacatatta tatatacaat taaaatttta
    31561 cttgatatat aattgaaatc aaagagaagg tgatacaatg ataactaaga aacatgcaat
    31621 aatagtaaag agtttaagca ataaagatgg atatatgaca tcaaacgaat tagctatcaa
    31681 attggatgtg tctactaaaa ctattaaaag atatatagca gatttaaata gtgttttaag
    31741 caaatatgat ttggtaatag cttcttctag aggtatagga tacaagttaa gcggttctaa
    31801 aaacaatata gccagagctg ttaaagaagc aaataaatat attgatggtt ttttagatga
    31861 ctctgaagag tcaagaatgt caaatataat atgtatgctt attaatagaa attatatgag
    31921 tatagaagca atggcagaag aattaaatct aagtatagcc gctataaata aattgtctag
    31981 taagcttaaa agaaaacttg aaaagtatga cttagttata aaatctaaac cttactatgg
    32041 aagctatata catggtgaag aaattaatat aaggcaatta attactgact atgcaataaa
    32101 attagatgaa aaaagtaaag tgaaggtgtt tttagatgac attagtgaaa acgatataca
    32161 ctgtatagaa agtattttag aaaagcactt aagagaaaaa gatacaataa tttcagataa
    32221 agattttaat cttttgcttt caaaaataat cgtttctgta tttaggagta agagaggtca
    32281 tagtaacaat ataaacttaa tggatacaag ttatagattt cataactact actttataga
    32341 aaatctaatg aaagaaatat ctgataaaat tggatttaaa cttattgaag atgaagtaat
    32401 ttatatttca aattattctg gtgtaatagc ctataaaggt acacaaggaa gaaaaaacac
    32461 aagtgaaatt gaggaacgaa taagtactgt tataagtagt gcacttcaag atatattcct
    32521 tatttcaggt agtgattata caaaagatga tgagtttatg gttgcaatct ctgaccatat
    32581 aaagagattt ttaaatagag ctagagctaa tgtaaaatca aataatcctt tacttcatca
    32641 aataaaagag aagtttccta tagcttttaa tttagccgtg tttttatcaa ataaactaga
    32701 aactgagttt aatttaaaac ttgatgaaga tgagcttgga tatatagcta tccactttgc
    32761 tgcttcaaat gaaagaatga aaaaaaatac tagtaagaaa atttgtatag tttgtcatta
    32821 tgggatagga acaggacaac ttatttctga aaagctaaaa cagaatataa gtgatttaag
    32881 tgttgtgggt gtttatccag ttagatatct cgatatggct ataagtcaag atgttgattt
    32941 gatagtatct accgttgaat taaaaggata tgaaaaacct gtcttatata tagaaaatat
    33001 atttgatgat agcttaattg aaaacgtaaa caaagccttt tatgaaaagg aagaaagaag
    33061 aaagataata agcaacatgt ttgatgaaaa agcttttttc agtataaagg ctaatactaa
    33121 agaagaggtc ataatttcac tttcaaataa actaaaagaa agagatttta tagaggaaag
    33181 ttctataaaa agtataatag atagagaaaa tatttcatca actgaaatag gaaatctagt
    33241 agcaataccc cacacaatag tgaagggaga taaaaaatct ataataggag ttggaatact
    33301 tgaaaatcca ataatttggg ataagcaaga ggttcagcta gtatttatgg tattttttaa
    33361 tactaaagaa aaacataatt tctcgatatt taaatacctt tataatttta taaaagatga
    33421 aggcggagta agaggaatta ttaaaatatg tgactttaat aaattaatgg cactgatagg
    33481 taattaagga ggaaattaat aatgggaaca aacatattta acaaagaata tgtattttta
    33541 aatgttgatg ctaagtcaaa ggttgaagtt ttaaaattta tatcaaaaaa agctaaagac
    33601 ttaaacttgg cagaagatga gagcttagta tatgaaggtt taatggctag agaagaacaa
    33661 tttactacaa atttaggaga atctatagca atacctcaca ctaaaaatga tgcaattgaa
    33721 aatccagcag tagtagtatt gaagtttaat gaagatgttg tttggaatga aggtgaggat
    33781 aaggttaaat tagcaataag cttacttatg ccaggaaaaa gtaaagagaa tattcatctt
    33841 aaattattat catctctttc aagaaaatta ataaataaag aatttaaaga ttctctttta
    33901 aaaagtgata atgttgaaga aatatctaac ttaataaatg aagctttagg tttataatta
    33961 aacactaagg ttataaaata aaaaaataat taactaaaaa taggaggaat ttgaaatgaa
    34021 taaaaaatta gtagcattat gtgcttgtcc aatgggacta gctcacactt tcatggcagc
    34081 agaagcaata gagcaagcag caaaggcatt aggttatgaa gcaaaagttg aaactcaagg
    34141 tgctgatggt gtacaaaatg agttaacaag agatgatata ttaggagcaa ctatgataat
    34201 acatgcagta gcaataacac cagaaggtat ggaaagattt gatggttgtg aagtttatga
    34261 agttgaatta caagaagcta taaaaaatgc agaaggtgtt ataaaagaaa tagaagaaga
    34321 tttaggaata taataaattt attgtggagg aaatagaatg gcaattaaga aaaaagttat
    34381 aaacagttca ggtgcttcta caacaggagg aaataatgta ggaaaatcta ctccatctag
    34441 tccaactcaa tcaaagggaa atggaaaatg gaaagaaata agtaaacata taatgacagg
    34501 tatatcatat atgataccag ttttagtaat gggtggactt ataggggcct tatctcaatt
    34561 aataccatat gctatattag gacttgaccc atctgtaggt atagttgatg ctatgaactc
    34621 aggagaattt acagggttca agcttagtct tttaaatata gcacaattaa tgtcaaattt
    34681 cggatttact ttgtttggat ttgcgatacc actgtttgca gcattttgtg caaactctat
    34741 aggtggaaaa acagctttaa ttgcaggatt tataggtgga tatatagcta ataaacctgt
    34801 aggtgtacca caattcgttg atggacaatg gactgaagtt gttccagttg catcaggatt
    34861 tttaggtgca atactaatag catttataat aggatatttt gtaaaatggt taaataaatc
    34921 tataaaagtt tctcataact ggttagcttt taaaacaaca tttttaatac cattaatagc
    34981 gtcattagct tgtatggtat tgatgatatt tataataact ccatttggtg gattaattaa
    35041 tgaaagtatg aaaaacttct taacagcagc aggagcagct ggagaatatg tatatgcaac
    35101 agctttggca gcagctacag cattcgactt aggaggacca ataaataaag cagcaggatt
    35161 cgttgcactt ggtttaacta cagagaatgt acttccaata acagcaagaa caatagctat
    35221 agttgttcct ccaattggat taggattaac aacattatta gataaaagat tagtaggaag
    35281 aagagtatat gatagacaat tctatcaagc aggaaaaact tctatattct tatcatttat
    35341 gggtatatct gaaggagcaa taccatttgc acttgagaga ccaggttttg ttataccttt
    35401 aaacatagtt ggaagtgtaa taggagctat tacaggtata attctaggag caattcaatg
    35461 gttcccagag tcagctatat gggcatggcc tctagtagat aacttatttg gatatataat
    35521 aggtatagca gttggtgcta tattcatagc agtaggaaat atattctata gaaataaatt
    35581 aataaaagat ggtaaacttg ttgtagatta tatcgactag tatttaattt ttactatcta
    35641 taaataactt tggctggtaa tatggtttta atcaatatta ccagccttac ctatatgatt
    35701 aattaaaggc ggtggagata agatatggat aagatagaaa aagtaagaga gtattttaga
    35761 gaatatgata tagatggatt tcttataaat agtcctacaa acaagtttta tgttggaaat
    35821 ttatttagct cttcaggtta tgtttttata actaaagaat cacaatatat aatagtagat
    35881 tttagatatt ttgaagaaat taaaagaaaa tctagtttgt ttaatgtagt attaatggac
    35941 aaaactagaa ctcattttga tataattaat gatatttgta gagagcagaa tataaaggaa
    36001 attggatttg agggaaatga agtatctttt gatttatata gaagtatgag taataagtta
    36061 agtgcaacct taaaatcagt tgatttatct acattaaggg aaacaaaaaa tgaagacgaa
    36121 ataaaatata taaaaaaagc ttgtgaaata gttgatgcta cattttatca tatagtagac
    36181 tttataaagg ttggtatgac tgaaaagcaa gttgaaaatg aaattgttag aattataaaa
    36241 gatttaggtg gtcaaaaaga atctttcgat acaatagttg catctgggct tagaggagca
    36301 ttacctcatg gaaaagcaag tgaaaaagtt atagagtatg gagattttgt tacctttgac
    36361 tttggtgcta agtataataa ttattgttca gatattacca gaaccatttg tatgggtact
    36421 ataaataaag agttagaaga aatatataat atagtaagaa aagctaatga agagtgtata
    36481 agggttttaa gaccgggaat gactactgga gagatagata aagttgcaag agacataatt
    36541 ggttcatatg gatatgccaa taattttggt cataatctgg gtcatggtgt aggaattatg
    36601 gttcatgaat atcctgcatt agctcctgaa tcaaatgaag tattaaaaga aggaatgatt
    36661 gtaactatag agcctggtat atatgttcca agtcttggag gcgtaagaat agaagatgat
    36721 gtacttataa cacaagatgg atgtatgaga ttgactacat ctactaagga tttaatagtt
    36781 gtaagctaaa aataaattag ataaatattt tataacaata agaaaagaag aggtaaattt
    36841 aaatgtataa ttttgatgat aaaccagata gagtaagtga aaagtgtaga aaatgggatt
    36901 taaatattat aagagataaa tttggagata ttagagaaga ttttatacca atgtggattg
    36961 ctgatatgga ttttaaaata ccaacagaaa tagaaaacaa atttattgaa gctgtaagaa
    37021 gaggtgtatt tggatataca tactgttatg atgaatttta tgattcagtt attggttggc
    37081 aaaaaaatat gcatgaggta aatgtagaaa aagaatggat tacattaact tatggaactg
    37141 tatcaacact tcattatgta gtacaagctt tttgtaagaa aggtgatagt ataatattaa
    37201 atacaccagt ttatgacccc tttgaatcat cagcaaaaaa acaaggtgta aatgtaattt
    37261 gtaatacatt agatgttgta aatgatagat attatataaa ttttgataaa ttagaagttc
    37321 aaattaaaga aaataagcct aaattaatga tgttttgtac accacataat ccatctggaa
    37381 ggatatggac aattgaagaa atgacaaggg tagcaactat atgtaaagaa aataatgtta
    37441 ttttggttgc agatgaagta catgcagagc atattcatta tggtacattt aattcaatac
    37501 ttaagattgg aaaagagctt ttagaaaatg ttattctatt gacatcacca aataaaggat
    37561 ttaatcttgg tggattaaag acttcttatt caatagttat aaataaggat ataagggaca
    37621 aatttagaaa taaattaaag caaaattcta taacttctcc aaatgtgttt ggaataatag
    37681 gattgataac cgcttataat gaatgtcatg agtggttaag tggtgtaaat gaatatataa
    37741 aatcaaatta tgaactttta gaaacttggg taaataaata taataagata aaattgatga
    37801 aaatggaatc atcttactta gcttggatgg atataagtgg attaggtatt agtgcaagtg
    37861 agtttacaga taaattggct atagatacag gagtattatt agaagatgga agtcattttg
    37921 taaaagatgg tgagaagtat gttagaataa atttaggaac tcaaaaggaa aatgtaattg
    37981 aagccttaaa tagaatggat ttatttttaa agtctttata aaaatttggt tgaattagta
    38041 ttttatataa atttaaacta ttaatatggt atatacataa gtagaatttg ttttagatta
    38101 cttaagta
//
LOCUS       DAELNY010000020        35657 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.020, whole genome shotgun sequence.
ACCESSION   DAELNY010000020 DAELNY010000000
VERSION     DAELNY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.020"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            150..290
                     /locus_tag="KPE55_000872"
     CDS             150..290
                     /locus_tag="KPE55_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491968.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(465..785)
                     /locus_tag="KPE55_000873"
     CDS             complement(465..785)
                     /locus_tag="KPE55_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0491969.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(905..1642)
                     /locus_tag="KPE55_000874"
     CDS             complement(905..1642)
                     /locus_tag="KPE55_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG0491970.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1857..3680
                     /gene="acd"
                     /locus_tag="KPE55_000875"
     CDS             1857..3680
                     /gene="acd"
                     /locus_tag="KPE55_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG0491971.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3895..8193
                     /locus_tag="KPE55_000876"
     CDS             3895..8193
                     /locus_tag="KPE55_000876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG0491972.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8397..8858
                     /locus_tag="KPE55_000877"
     CDS             8397..8858
                     /locus_tag="KPE55_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG0491973.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEIEFNRKDVAIVRLAIKF"
     gene            8888..10042
                     /gene="nusA"
                     /locus_tag="KPE55_000878"
     CDS             8888..10042
                     /gene="nusA"
                     /locus_tag="KPE55_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG0491974.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10049..10330
                     /locus_tag="KPE55_000879"
     CDS             10049..10330
                     /locus_tag="KPE55_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG0491975.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10320..10631
                     /locus_tag="KPE55_000880"
     CDS             10320..10631
                     /locus_tag="KPE55_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG0491976.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10653..12593
                     /gene="infB"
                     /locus_tag="KPE55_000881"
     CDS             10653..12593
                     /gene="infB"
                     /locus_tag="KPE55_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG0491977.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12636..13016
                     /gene="rbfA"
                     /locus_tag="KPE55_000882"
     CDS             12636..13016
                     /gene="rbfA"
                     /locus_tag="KPE55_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG0491978.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12997..13980
                     /locus_tag="KPE55_000883"
     CDS             12997..13980
                     /locus_tag="KPE55_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG0491979.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13993..14898
                     /gene="truB"
                     /locus_tag="KPE55_000884"
     CDS             13993..14898
                     /gene="truB"
                     /locus_tag="KPE55_000884"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG0491980.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15029..15958
                     /locus_tag="KPE55_000885"
     CDS             15029..15958
                     /locus_tag="KPE55_000885"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG0491981.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16062..16319
                     /gene="rpsO"
                     /locus_tag="KPE55_000886"
     CDS             16062..16319
                     /gene="rpsO"
                     /locus_tag="KPE55_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG0491982.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16559..17404
                     /locus_tag="KPE55_000887"
     CDS             16559..17404
                     /locus_tag="KPE55_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0491983.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17529..19640
                     /gene="pnp"
                     /locus_tag="KPE55_000888"
     CDS             17529..19640
                     /gene="pnp"
                     /locus_tag="KPE55_000888"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG0491984.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19906..20649
                     /locus_tag="KPE55_000889"
     CDS             19906..20649
                     /locus_tag="KPE55_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0491985.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20807..22054
                     /locus_tag="KPE55_000890"
     CDS             20807..22054
                     /locus_tag="KPE55_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0491986.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22089..22334
                     /locus_tag="KPE55_000891"
     CDS             22089..22334
                     /locus_tag="KPE55_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0491987.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22344..23567
                     /gene="dapG"
                     /locus_tag="KPE55_000892"
     CDS             22344..23567
                     /gene="dapG"
                     /locus_tag="KPE55_000892"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0491988.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23680..24414
                     /locus_tag="KPE55_000893"
     CDS             23680..24414
                     /locus_tag="KPE55_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG0491989.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24537..26948
                     /locus_tag="KPE55_000894"
     CDS             24537..26948
                     /locus_tag="KPE55_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG0491990.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26951..28009
                     /gene="mnmH"
                     /locus_tag="KPE55_000895"
     CDS             26951..28009
                     /gene="mnmH"
                     /locus_tag="KPE55_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG0491991.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28003..29337
                     /gene="rimO"
                     /locus_tag="KPE55_000896"
     CDS             28003..29337
                     /gene="rimO"
                     /locus_tag="KPE55_000896"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG0491992.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29324..29866
                     /gene="pgsA"
                     /locus_tag="KPE55_000897"
     CDS             29324..29866
                     /gene="pgsA"
                     /locus_tag="KPE55_000897"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG0491993.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30154..31200
                     /gene="recA"
                     /locus_tag="KPE55_000898"
     CDS             30154..31200
                     /gene="recA"
                     /locus_tag="KPE55_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG0491994.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31376..32917
                     /gene="rny"
                     /locus_tag="KPE55_000899"
     CDS             31376..32917
                     /gene="rny"
                     /locus_tag="KPE55_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG0491995.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33231..35567
                     /locus_tag="KPE55_000900"
     CDS             33231..35567
                     /locus_tag="KPE55_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0491996.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT        14382 a         3795 c         6736 g        10744 t
ORIGIN      
        1 aaaatatcct attgttttgt agatatatcc taattatgtc aaaaaataaa aaaattttta
       61 tttattagag tttttggaaa taataataac agatattata attaattaac agtttttgta
      121 agtatttgac aatagagagg gtgtaacaaa tgggaataat agagtcagca tcaaaattgg
      181 ctgagatggt tcatttattg gctgttgaaa aggggataac tgatatagaa gcatgggatg
      241 aagctgtgaa agaatacagc aaaatttatg aagaaagaag aaatgaatag aatgataaaa
      301 aatgaaaact tgatgataaa aaataacttt atttaaagaa attcaatttt tatagaagta
      361 ttaaaaataa tataaataaa aagatggtat ttcaaatgta agtaaaaaat cattttggga
      421 taccatcctt attatttatt ttaataatat ataagtaata aatcttactt atccatattt
      481 tttatgtaac ctcttttttc tagctttttt atatgctcct tacgtatttc atcaagatta
      541 ataccatatt gttcttctag cttaaacatc atagtaacac atgtctgcgc tacatccatt
      601 agttccttag cagttttctt tataatatca acttcatcta aatcattatt ttctccactt
      661 agatttctga atttacctat acattcagct aattcacctt gttcttcacc cattttgata
      721 aatgtagatt ctagtgttgg gtctaaatta ttaagctctg gtaaagatat agtttttagt
      781 ttcataaaag ctcctttcaa gttattcgta tttatactaa aacatcataa caaaaataat
      841 actaaaaaaa aactttaaat tcaacataaa aacttacata tataataaaa atcgtgaaat
      901 aaatctatat ttgtcttaat acagcctcat ataaaacttc tccaataggt ttatttatta
      961 caagagaagc tgacttatca tagactgtat ttgctttatt aataagagca atataatcac
     1021 ctttgtaata atctataaaa cttgctgctg ggtacacagc taatgatgta cctcctataa
     1081 tcaataaatc agcattactt atagcagaaa ttgttttagt aatgacatca ctgtcaagag
     1141 cttcctcata aagtactaca tctggtttta ctatagaacc acagttgtca cagtaaggaa
     1201 tattacctcc cagatttaac atagattcta agtcaaaaaa cttaccacat ttagtacaat
     1261 aatttctatg tacagaacca tgaagttcta gcacattttt acttccagcc atttgatgaa
     1321 gtccatcaat attttgagtt atgacagctt taagcttacc catttcttcg agttttgcta
     1381 aagctatatg agcattattg ggttttgcat taggatatat taatttatcc ttataaaatt
     1441 caaaaaattc ttctggatat ctaacaaaaa aagtatgtga aactaattgt tcagcagtaa
     1501 attgtttatt gagtttttgg ctaaataatc cagtagaact tctaaagtct ggaatattag
     1561 attcagttga aacacctgct ccaccaaaaa atacaatatt attgtgatta gctattaagt
     1621 cttttaagct atttgcatcc atagttacac ctccaaatta ttatatataa attgtactac
     1681 atattaggtc gtttatgttt tattaattta aataaatata tagtttaata agttaaataa
     1741 agggaatata aataaaatgt atacagagta taaatagaca atatttacta aaataacaag
     1801 ttgtcgtata attatattaa aattgtttta gtgtataaga agggcggggg aagtacgtga
     1861 agaaaaaagc agctttagca acattggcta tgttaccctt aggtgtggta aatgcacatg
     1921 ctgatggaga tataggtata gtgactataa attatttaaa tgtaagaaat gaaccaactg
     1981 ccgaaagtag catagccttt gttgctaaaa aagatgataa agttttgatt aaagactctt
     2041 ctaatggatg gtataaaata aaagctgaat ctggacaaga aggttgggct tcatcaaaat
     2101 atatagcaaa atcaaatagt gactctttaa gaacgtctac aaataaagaa aaacaagtta
     2161 tttcaaatag tttaaatatg agaaatggag caggtacaag ttacagagta ataactgtac
     2221 taaaaaaagg tcaaaaagta gaagtaatat cagagagtaa tggatggtct aaaatcaaat
     2281 atgatggaag actaggatat gtatctagct cttatttagg agacgtttca aactcaacta
     2341 ataaatcaaa aactaaacaa gtgaatacaa cttcactaaa tgtaaggagt ggaccaaata
     2401 caagttatgg tttgttaggc aagttgccaa aaggaagtaa agtagaagta atatcagaaa
     2461 gtaatggatg gtcaaaaata aagtataatg gaaaagatgc ctatgtatct agtatgtact
     2521 tatcggatgt aagtcaaagt aattcagaca attctagtca aagcaatgat aaaaagaata
     2581 ctgataaggt tgtaaataca gcttctttaa atgtaagaag tggaccaggt tctacatata
     2641 gtaagttggg aaaagtttat aaaggaagta aagtaactgt actatcagaa agtagtggat
     2701 gggctaagat taattttaac aataaagaag catttgtagt aggcaattat ttatctactt
     2761 cagcagatac ttcaaataat aactcaaata gtaactctga taacagttct aatagtaatg
     2821 gtaataattc ttcatcatca ggtcaagtaa atggtatgtc aggtataagt ggggctaaaa
     2881 ttgattataa atctttgagt tatactttag aatctcatat aagtaaacaa gttgagaaag
     2941 cagcatcagg aggaaatgtg atagctccaa gtaataggaa aagtactcca agtcctgaat
     3001 ttagtacttt ttcagctcaa agaacaagct catttgtaaa tgcaagttca agtgatatag
     3061 aatattattt aaatcctaag aattttacaa atactactaa aggtatgatg cagtttttaa
     3121 agattaacag ttatagagat ggtatttcag aatcaagtct taactcgtat cttaatggtt
     3181 tatcttctag tgtatttaaa aatcagggag cagcttttat aaatgctgcc aagaagtata
     3241 atatagatgt tgtatatcta gtatctcatg ctatgtggga aactgcttat ggtaagtcta
     3301 cacttgccca aggtcaaaca ttaacatctt ataagggaca acctcttagt aagccagtta
     3361 aagtatacaa tttttttggt ataggagcaa tagataaaag cgctaatgtt tcaggtgcag
     3421 aagcagcgta ttccaatggc tggactagtg tagaagctac aatagatggc tctgcaaagt
     3481 ggatatcaca aaattacgta aatagttcta agtacaatca gaatactatc tacaagatga
     3541 agtggaatta tgattataca tggcatcagt atgcaactga tgtaaactgg gctaatggaa
     3601 tttctggaat tatggaaaat ttaattggtc tttatggagg aggaagtagt ttggtttttg
     3661 aagttcctca atataagtaa tacataaata aaaaatatag actatgggta gttttcattt
     3721 tatttaaatt atctatagtc tttttgtata taatttataa tctgtaaatt caatgttaac
     3781 aaaactttta tgttataata ttaattctaa gtaaatataa gtgagtaata ggtagaaata
     3841 gataaaaagt ggtaataata tatataaaga taattttgag aaaggaggat atatatggaa
     3901 agtattaagg aatatttgga taagttagaa attaataata gtggtcttgg aaagcaactt
     3961 aaagaagttt atataaatag agtagtttat tttaaggaag ataagatagt ttatttttat
     4021 ctaacttcta aagatattgt ttctcatgaa cttttagata aatttaaaga agaattgatg
     4081 tataaactcg attattttaa agatatgagg atgaagataa gatttactgg tcttgaaaga
     4141 aaatccaata aagatgttat aaaaaaatat tggaataata tattgtatat attaaagtat
     4201 ctttgtccat ctattgcagg gtggtataaa caagtagaat ttctttgttt agaagaagaa
     4261 ttaaaaataa aacttccaaa aggtatattt tatgagcgat taatgaaaaa aaatgtaact
     4321 tatgtactta aaaccgtgct tagcgaagag ttgggattag atttaaatat aacaatagaa
     4381 aaagctgttg atgaaaaggt aaataaagaa agactcataa gaatcaatga tagagaaatg
     4441 gaagaaaaaa ttagggcttt agaaattgga aaagttaata actgtgaaga aaatgaagag
     4501 gaaagctatg tcattaagtc tgatgtagat gaaaatttaa tctatggaga taatgcaaat
     4561 gctatgattg aaaatatagt tgaattaaat gcatcttctg gaacagttgc agttgttgga
     4621 gatatatttg atgttgacac taaagaactt aaaaacggaa aaatacttat gatagcttca
     4681 attacagatt atactagctc aataagctgt aaactttttt taacagatac aaataaggat
     4741 ggagtacttg aaaatgttaa aaaaggagca tacttaaaaa tcaagggtga catagtttat
     4801 gatacatatc aaagagaaat tagtatgatg attagtggga taagaaaaga aactagaata
     4861 gagagagaag ataaatcaga ggaaaaaaga gtagaactcc atgcacatac acaaatgtct
     4921 tctatggatg ctatttgttc tgttaaaaag cttgtagagc gtgcagccaa atggggtcat
     4981 tctgctattg ctataacaga ccatggtgtt gtacaaggat ttccagatgc tatgaatgca
     5041 ggtaagtcaa ataatataaa aatactttat ggagtagaag ggtacttggt tgaagatgat
     5101 tctctcatta tagaggatgc aaatgataaa gaactttcac agacatttgt tgtatttgac
     5161 atagagacaa ctggtttttc taatacaaat gataaaataa ctgagattgg tgctgtaaaa
     5221 atagaaaact ttaagattgt agatagattt agtgagttaa taaatcctga aaaagatatt
     5281 tcttataaga tacaggaatt gacaggaata actaatgaat taattaaaga caagccaacc
     5341 attgaagaag ttcttcctaa gtttatggaa tttgttggag atagtgtatt agtagcacat
     5401 aatgcagagt ttgatactgg atttatatcg caaaaatgta gggaacaagg cttgttatat
     5461 aataacaaaa aagtagatac acttatgtta gcaagagtaa tgttgccaaa cttaaaaaga
     5521 catagattaa atgtcgttgc aaaagaactt ggtattcctc ttttaaatca ccatagagca
     5581 gtggatgatg ctgaagctac agcacttata tttaataagt ttttgcagat gcttacagaa
     5641 aaaggtgcaa aaactctaag tgatgtaaac aatatacttg gaaagattga ttacacaaag
     5701 cttagcacaa atcatataac cttgatagca aaaaattcta taggtataaa aaatttatat
     5761 aaaataattt cagatgcaca tgtaaatcat ttttttagag caccaagaat ccttagaagt
     5821 gtattaaatg aatataaaga aggattaata attggttcgg cttgtgaagc tggggtggtt
     5881 tttcaagctg taaagaaaaa cgtgtctgat gaggaaatga aaaaaataat agatttatac
     5941 gattatattg aagtaatgcc tatagataat aatagattta tgattgataa aggagaagta
     6001 aaggacgaag aagagttaag ggagttgaat aggaaattaa ttgatacagc caaaaaattt
     6061 gacaaaattc cagtagctac tggggatgtt cattttttag acaaacatga agctgtctta
     6121 agaaaagttc ttaaatattc tcaaggtttt aaagtagatg aagaagaaac ttatttacat
     6181 tttagaacaa cagatgaaat gttggaagag tttagttatc ttggagaaga attggcatat
     6241 gaagttgtag ttgaaaatag taatctgatt gccgatatgg ttgaagatat aaaacctata
     6301 ccagatgata cttatcctcc aattatagag ggctcagatg tggagttaag agaaatgtgt
     6361 tacaaaaaag ccaaacgaat ctatggagac ccaataccag aaatagttca aaaaagactg
     6421 gatagagaat taaattcaat aataagcaat gggtatgcag ttatgtatat tatagctcaa
     6481 aaactggttg caaaatctct ttcagatgga tatttagttg gttcaagagg ctcagttggc
     6541 tcatcttttg cagctactat gagtgatata acagaagtta atcctcttcc agcacattat
     6601 atttgtgaaa acgaagactg taaatattca ttcttttatg aaataggtga atggggctca
     6661 ggtgttgatt taccagacaa ggattgtcca aagtgtggaa gaaaacttgc taaaaacggt
     6721 catgatattc catttgaggt tttcttaggt tttgaaggtg ataaagaacc agatatagac
     6781 ttaaatttct caggaaatta tcagccaaca attcataaat acacagaaga actatttgga
     6841 gaggggtatg tatatagggc tgggactata ggtactgtag cagaaaaaac agcttttgga
     6901 tacgcaagaa aatatgtgga agaaaataat ataagtgtac caaatgcaga agtacttagg
     6961 ctttcaaatg gatgtactgg agtaaaaaga acctcaggac aacatcctgg tggagttatg
     7021 gttataccag actataaaga tgtatacgat tttacaccta tacagtatcc agcaaatgat
     7081 acttcttgtg gtgttataac tactcacttt gattatcatt caataagtgg tagaatactt
     7141 aaacttgata tacttggaca cgacggtcct accattataa ggatgcttga agatatgact
     7201 ggaataaata taacagaaat tcctttagat gataaagaga ctatgtctct atttacatct
     7261 acagaagcat taggtgttac accagaagaa ataaactgtc ctataggttg ccttgctgta
     7321 ccagagtttg gaactaagtt tgtacgacaa atgttacttg acacaaaacc tactacattt
     7381 gcagaacttg tacgtatatc tggtctctct catgggacag atgtttgggt aaataatgca
     7441 caagacttag ttagagaaga tatagttgga ttaaaagatg ttatatctac acgagatgac
     7501 ataatgaact atttaatatt taaaggatta cctcccaaaa tgagctttac aataatggag
     7561 agtgtaagaa aaggtaaagg tttaaaacca gaacacattg aagaaatgaa aaaaaataat
     7621 gttccagaat ggtatatagg ctcatgtaaa aagattaagt acatgttccc aaaagcacat
     7681 gctgttgctt atgtaatgac atcatttaga attgcatatt gtaaagttca ctatccagaa
     7741 gcattttatg cgacttattt tacgacaaaa gttgaggatt ttgatgcaga tttaattgtt
     7801 aaaggattag atgctataaa atcaaaaata aatgaaattg agtcactggg aaatgatgca
     7861 accacaaaag aaaaaggtat gttgaccgtt ttagaagttg ctcttgaaat gtatgctaga
     7921 ggaataaaat tattaccagt tgatatatat aaatcagatg caactgagtt tatagttgta
     7981 ggagaaaaaa cattacttcc accaatggca gcaatacaag gtcttggagg aaatgcggct
     8041 ataaacatcc aaaatgaaag aaaaaatggt gaatttatat caaaagaaga tttgagaaag
     8101 agaacaaaaa tatcaaaaac ggtaatagaa actcttacta atcatggttc acttgagaat
     8161 atgagtgaga gaaatcaatt gtcattgttt taagttagcc caaaaagttg caaataaaag
     8221 aattttatga tatcatagta tagaaaatag tatactttgc agaaactaag agtgagacag
     8281 ctgttctcac tcttttattt taaatatatt attaaaaagc tggtttattt ttattaaata
     8341 tattttattt aagattatat aaactggtca tttacaattg aataggagga actttaatga
     8401 aaaaaaattt agaagctaca attgaggaga ttgtaactaa aataacggat gaacatggat
     8461 ttgaaatggt tgatgttgaa tatgtaaaag aagcaggaga atactactta agagtataca
     8521 tagataaaga agaaggaata tctttaaatg aatgtgaatt agttagtaga gaattaagtc
     8581 caatactaga tgaaaaagac cctataaaag aaaattactt tttagaggta tcttctccag
     8641 ggcttgatag agcattaaaa aaagacagag attttgttag atatcaaggt agagatgtag
     8701 atttaaaatt atataaacca ttaaatggat gtaagcaatt tgaaggtgaa cttgtaggat
     8761 taacagaaga taataatata aaaatcattg caaatggtaa ggaaatagaa tttaatagaa
     8821 aagatgttgc tattgtcaga ttagcaataa agttttagtg gtatattaaa tagggaggat
     8881 ataaaaaatg aatcatgaat ttatggaagc attagatgaa ttagtagttg acagaggtat
     8941 agataaagaa atacttatag atactataga acaagctctt ttaactgcat acaagaaaaa
     9001 ctttggttca gcacaaaatg ttagagttga atttgataga gacaagggag atataaaagt
     9061 attttctcaa agagttgttg ttgatgaatc agatttatat gatactttct tagaaataga
     9121 gttagcagat gctagagaga taaaccctaa ttatgaatta ggtgatatta tagaacatga
     9181 agttacacct aaaaactttg gaagaattgc agctcaaaca gcaaagcaag tagttgttca
     9241 aagaataagg gaagctgaga gagaaatagt gtataatgaa tttatagaaa aagaaaatga
     9301 aatagttaca ggagaggttg ctagagcaaa taaaaatgtt gtttatgtaa atcttggaag
     9361 aatagaaggt ataatgactc aaagtgaaca aataccattt gagaattata aagcaggtca
     9421 aaaaataaaa ttctatatac ttgaagttaa aaagactaac aaaggacctc aaatagttgt
     9481 atcaagaagt catccgggtc tagtaaagag attgtttgaa gaagaagttc ctgaaatata
     9541 tgaaggaata gttcaaataa aatctgtagc aagagaagca gggtcaagaa ctaaaatggc
     9601 agtaagatct atagatgaaa aaatagaccc aattggagct tgtgttggac caaaaggact
     9661 tagagtaaaa aatatagtgg atgaacttgg cgatgagaaa atagatataa taaaatatag
     9721 tgaagaccca gcagagttta tatcagcagc acttagccca tcaaaggttg ttagagtaga
     9781 tatagatgaa gatgaaaagt ctgccttagt aatcgttcca gattatcagt tatcgcttgc
     9841 tataggtaaa gaaggtcaaa atgcaagact agcagcaaaa ttgactaact ggaaaataga
     9901 tattaagagc gaatctcaag cagaacaaga aatgttaaat cttgaaaatt ccaaagaaat
     9961 agaagctgag gatgttgaag ctgaatttat cgaccaagaa gagttagata cttcagagga
    10021 taattttagt gaagaagagt aaggagggat gattttgaaa aaagtaaaaa aagtgcctca
    10081 aagaaaatgc atagcatgtc aagatagaga ctctaaaaaa gagcttataa gaatagttaa
    10141 aaataaagaa ggaaaaatat ttttagaccc aacaggaaga gccaatggaa gaggagcata
    10201 tgtatgtaaa agctctgaat gtctaaaaaa agctataaag agtaaagcat taaataaagc
    10261 ttttaaaata gatgttcctg atgaagtcta tgataatcta cttatggagt tagaagaata
    10321 tgaaaaataa taaagaaaaa gtactttcgt tcttagggct cgcaactcgt gcagggaaaa
    10381 tagtgtctgg tgatgattca actttgcttg accttaaaaa aggaaaagta aatttgattt
    10441 taatcgcaga agatgcatca aataatacta aaaaactttt taaagataaa tcaactttta
    10501 gaaatattcc atatctattt ttttcaacaa aagaggaaat aggatttgca ataggaaaat
    10561 ctccaagagc agttgtagga ataaaagatg aaaatttttc taaaaagata attgaactaa
    10621 tagaaattta aaataaaata gggggtgatc ttgtgtcaaa aacaagagta taccaaatag
    10681 cagaagaatt aaatatatca aatgaagagc ttataaataa gttagctgaa ttagatataa
    10741 atgtaactga caaagattca gtattagaag gtgaagagtt agaattagca cttgaaatgt
    10801 taggtgaaga tttatctcaa gaaaatggaa atgttataga aatagatgga aagttgacag
    10861 tacaagtatt agcaacgaaa ttagataaat ctccatcaga aataattatg aagcttatga
    10921 aaatgggaac tatggcaaca ataaatcaag agataagttt tgagattgct gctttggctg
    10981 ccaaagacta tggatttgaa ttaacagtag ctgaaagtga tgatacagaa gctttagaaa
    11041 tagaagcttt aatggaaatt gaagaagata aagaagagga tttaaaacct agaccaccag
    11101 ttgttacagt tatgggacat gttgaccatg gtaagacatc tttactagat gcaataagaa
    11161 aaactgatgt gatttcaggt gaagctggag gaataactca gcatataggt gcttcagaag
    11221 ttaaaatcaa tggacacaaa atagtatttt tagatactcc aggacatgag gctttcacat
    11281 caatgagagc aagaggagca caagttacag atatagcaat attagtagtt gcagcagatg
    11341 atggtataat gcctcaaaca gttgaagcta taaaccatgc taaagcagca ggtgtacctt
    11401 taatagttgc tattaataaa atagataaac caggtgcaaa cccagataag gttaaacagg
    11461 aattagcaga ccaaggatta ttagttgaag actggggtgg agaagtaata gcagttccag
    11521 tatcagctaa gaaaaaagaa ggaatagata cacttttaga aatggtatta ttggttgctg
    11581 aaatggaaga gttaagagct aatccaaata aaagagcagt aggaactgtt atagaagctg
    11641 agcttgataa aggaagaggg ccagttgcaa cagtacttgt tcaaggtggt actttaacag
    11701 taggtgaccc aatcgttgca ggtgttgcat gtggtaaagt tagagcaatg ataaatgcaa
    11761 aaggtaagag agtaaaaaca gcaggaccat ctacagcagt cgagatatta ggtctatcag
    11821 aagttccaca aggaggagac caatttgtcg aagttccaac tgataagatt gctagaagtg
    11881 tagcagcaag aagacaacaa atagttagag atgaaatgtt aaaatcaact caaagattat
    11941 cactagatgc tctatttagt caaatgagtg aaggttctat aaaagacctt aatatagtta
    12001 taaaagcaga tgtacaaggt tcagtacagg cagttaaaca atctttagaa aaattatcaa
    12061 atgaagaagt tcaagttaaa gtaatacatg gtggtgttgg agctgtaaca gaatcagaca
    12121 tattacttgc agcagcttct aacgcaataa taataggatt caatgtaaga ccagttccag
    12181 gtgcagaatc tctaggagaa aaagaaaatg ttgatataag aacatatact ataatataca
    12241 aggcaataga agatatacaa gcggctatga ctggtatgtt agaccctgaa tatgttgatg
    12301 aagaaacagg aaaagctgaa ataagagaaa tatataaaat atctggtgtt ggaacagtag
    12361 ctggatgtta tgtgactaat ggtaagatat ttagaaattg taaagtaaga ttagttagag
    12421 atagcataat aatacatgaa ggtgagcttg cagcacttaa gagatttaaa gatgatgtaa
    12481 aagaagttaa ttctggatac gaatgtggta tgagttttgt aaattataac gacataaaag
    12541 aaggcgatat agtagaagct tatataacta aagaagtaga aagaaaatta taaattcgta
    12601 tcaaagaaaa tcagttagta aagaaggtcg atggtatggc atcatataat agaacaagaa
    12661 ggatagcaga agaaataaga aaagtcgtaa gcactatgct tataaatgga gtaaaagacc
    12721 ctagaataac atctatggtg agtgtaactg atgtagaagt tacaaatgac ctaagatatg
    12781 cttatgtata tgtaagcatt ctaggtggag atgaagaatc cacactaaca ggtcttaaaa
    12841 gtgctggagg atatataaga agagaagtag gaaaaaatat aaaacttaga tatattcctg
    12901 aaatagtatt taaacttgat gattcaatag aaaaaggaat gtatatggat agccttataa
    12961 aaagagttaa tgaaaaaaat gctcaacaaa atgaggatga aaattatgat gaataatatg
    13021 gatagtatta taaactatat ttatgagggt gactatttta tagttacctc tcatatcagt
    13081 ccagatggag acaatattgg ttctacactt tcaatgtatt atacattgaa gaatttagga
    13141 aaaaatgtat attatgtact tgatgatgaa gctccattaa atttaaggtt tttggttgaa
    13201 gatgtaaaaa tctataagtc taatgaattt aatatgaaga actattctct aattgcactt
    13261 gattgtgggg ataagatgag aatatgtgtg agtgatgaaa tcaaaaataa tgcatcaaaa
    13321 ttaatctgta ttgaccatca tgctagtaat gattcatatg gagatttaaa ttatgtagat
    13381 attgatgcat cttctacatg tgaattagtt tataatttat tagttagatt tcagcaaata
    13441 aaagatatta atattataaa tgaagatata gcaacatgtt tatatacagg tcttgtaact
    13501 gatactggaa acttttcgta ttcaaatgta catgcaagta gctttgaaat ggcaaaaaat
    13561 ttacttgttt taggtgcaca aaaaaatact ataatacaaa atatatatca aagcaattct
    13621 tctggttatt acaaattatt aggagaagct ctaaagggtt tggagatatt tgattcaaag
    13681 gtatctagta tagttttaac tcaagacatg atgaatagaa ataatattag ctttaatgat
    13741 gttgatggaa taacaacata tacaagagac ataaaaggta tagaagttgg aattttattt
    13801 aaagagaaaa aacaaaatga aattaaagtg agttttaggt caaaaaatta tgttgatgta
    13861 agtgaaatag ccaagttatt tggtggtggt ggtcatgtaa gggctgctgg atgtactatt
    13921 agagatagta ttgataatgc aaagaaaatg gtactagagg cagtgttaaa atcaatttaa
    13981 ggtgacagta aaatgaataa gattataagt atattgaaac ccacaggtat gacatctcat
    14041 gatgttgtaa gtagagtgag aaagatttta aatataaaaa aagttggtca tacaggtact
    14101 cttgacccag atgcttcagg tgttttacca atctgtatag ggaaagctac aaaagtatgt
    14161 gaagttattt taaacaaaga taaatcgtat atatgtgagc ttactttagg tataagtaca
    14221 gatacttatg atgcttcagg tgaaatttta aaaaaagttg atgattttaa atttagtaat
    14281 gaagacatag aaagagcttt tgacacacaa agaggagaga taaatcaact tcctccaatt
    14341 tattcagctt taaaggtaaa tggaaagaga atgtgtgatt tagtaagaag tggtagacaa
    14401 tctgaaatta ctttaaaaac aagaagagta aatataaaag acataaaaat actaagtata
    14461 aaaggtaata aagtaatgtt ttatgttgag tgttctaaag ggacttatgt gagaagtatt
    14521 tgccatgata taggagaata tctaggttgt ggagctcata tgtcgttttt gaatagaaca
    14581 tcttctggta aatttgactt agataattca ataacattag aagaattaga attattttat
    14641 gaaaataaaa ctttagacaa atacttatat gatatagatt atgtccttga ttcgtttaat
    14701 tatgttgtat taaaccctaa tgctataaaa tattatagta atggtggctc aatagatgat
    14761 aaaaggtttt taaagaataa tttcgataaa gatgatgaat ttgtaagagt atacagtaca
    14821 gataatttct taggattagg aaagttatca aaacacaata atacaataag tgttaaaagt
    14881 gataagatgt tcatataacc aatgtattta aaaaaacaac aaaagagata gtactataat
    14941 aacttctata gtctatctct tttacttatg ctataatagt ttgtgaataa gttaagagta
    15001 aattatattt tagcggggaa catttattat ggtgattata aaatctataa aagaaatagc
    15061 aaacataaaa gaaagtgtca ttaccatagg aaattttgat ggtgtacaca aaggacatca
    15121 agttcttata ggaaaaacag ttgaacatgc aaaaaaagaa aatatacaga gtattgtatt
    15181 tacatttgca aatcatcctg taaattactt tagaccaaac tcaacaaaaa atattataag
    15241 caatgatgat aaaataaaat tattagaaga tttgggaata gatatagttg taatcatacc
    15301 atttgatgag tatatgacta aaatacctgc aaaggatttc gtagatgaga tactagtaaa
    15361 aaaattaaaa gctaaaaaga tagtaattgg acatgacttt acatttgcaa gggcaaaaga
    15421 aggcaatgtt aatctattaa aaagcttaga acatgaattt ggttttgagg tagaagtaat
    15481 aaaacccatc aaaatcaatg atgtaagagt gagtagtact tatattagaa gtttagtatc
    15541 ccagggtgat atggctaatg tcaaggaata tttgggtaga aattacaagt tagaaggttg
    15601 tgttatacat tctaaacacc ttggaagaac tataggattc ccaacggcaa atattgacct
    15661 taagaataat atgctcgttc caaagagagg tatttatgct tctattgtac atattggaga
    15721 tgagacttat tttggtgcta caaatgtagg ctataaccca acagttaatg gaaaaacttt
    15781 gtctattgaa acaaatattt tagagtttga tagagatatt tatggggaaa atataattgt
    15841 tgaatttttg gaaagaataa gagatgaaag aaagtttaat tcaatagatg acttaaaaaa
    15901 tcaattgtat aatgatacaa attatgtgta tgaaaactat gtttgcaaaa acaagtaatg
    15961 tatgataaaa tgattaagga aaataaccat tactcggcat ttgaatactc caatcaatgc
    16021 ttagtaatag gcgtttctaa aaaaatacgg aggtaatcta aatgataaac aaatcagaaa
    16081 taataaaaga atatggaaga gctgaagggg atactggttc tccagaagta caaatagctt
    16141 tattaacagc tagaataaac gagttaaatg gacatttaaa aactcataag aaagaccacc
    16201 attcaagaag aggactatta aaaatggttg gtagaagaag aaacttatta gcttacttaa
    16261 aggaaaaaga tcttgaagga tatagagcat taatagctaa attaggatta agaaagtaat
    16321 ttcattcttt ataaaaaagc aggtatagtg tatacctgct tttttatagt taattcatta
    16381 taaagtaaaa tagttaataa taaaagtagt ttatatgtta taaaaaatta taaaatgaat
    16441 aatattgggt aagaatatta aaatagtata caaatacata atcaatttaa aaggaaagac
    16501 aggataaata tagaaatttt ataacaggat aattagttgg aataaggagg atattatttt
    16561 gaaaataaag ttagtatgcg atagtttgtg cgatattcca gatgaaatat cagaaaaaga
    16621 ttatatagag atagttccac ttacagttat ttttaatgat agggaatata tagaaggagt
    16681 agatataaag aaagaagagt tttataaaaa agtaaaagaa atcaagcaaa tacctaaaac
    16741 ttcacaagct acatatatgg agtttaaaga agtgtttgat aagttcataa cagaaggtta
    16801 tcatataatc tgtatgacag gggctgcaaa cgcatcagga acttttcaaa gtgctatgat
    16861 agctaaaaat gatgtaaatg aaaatgaaaa aatacatata tttgatacta gaaatttatc
    16921 tctaggtagt ggacaatatg ttataaaggc atgtgaactt cttgaagaag gactgggatt
    16981 tgaagaaatt atagatgaat tagaaaatac tcgcagtagt gtaagattgc tttttgcacc
    17041 atatacactt gattttttaa agcagagtgg aagagttcca gttgcaactg ctttgatagg
    17101 aaatatgtta aatataaagc ctatattttt ctttgataat ggggaagcta agttggtaaa
    17161 taaggttaga ggaataaaga atatagcaag taaattggtc gatataattt tagaaatgaa
    17221 tgaagggtct ttagaaggaa aaatcgtcac aataggttgt ggagataatt tacacgattg
    17281 tgaaatatta aaagatgaag taaataaaag aataaaagca agaagagtat tatttactcg
    17341 aggaggagtt tctatttgct ctcatacagg gccagatata ttggctataa gttgttctaa
    17401 ttagtagtaa tataaggttt ttgcatatat atgtgaagat aattttatat tgttaattta
    17461 tagtattttt gataatatag taaatagagt gatatacgtg ttttttaata agaggaggac
    17521 aagcattaat gtttgaacat aaaatattta aaatggattt tgcaggaaga gaactttctg
    17581 tagaaatagg aaagatttgt gaaatggcta gtggtagttg tatagttcga tacagtgata
    17641 gtatggtaat ggtaaataca actaaatcag ccaaacctag agacggaata gacttttttc
    17701 ctttaagtgt ggattatgaa gaaaaattat actctgtagg taagatacct ggaggttttt
    17761 taaagagaga aggcaagcct tcagagaaag ctatacttac atcaagattg atagatagac
    17821 caataagacc tttattccca aaaggattta gaaatgatgt tcaggtagtt gcaacagttc
    17881 tatcagtaga ccaagattgt acaccagata tagtagcaat gataggttct tcaatagccc
    17941 tttctatatc agatatacca tttaatggac caacaggttc agtatgtgtt ggccttgtag
    18001 atggtgcatt tgttgtaaat ccaaacgcag agcaaagaga aaaaagttca atgcatctag
    18061 ttgtttcagg tacaaaggaa gcaataatga tggttgaagc cggagctgat gaagttccag
    18121 acgaggtaat gcttgatgct attttatttg ctcaccaaga aataaaaaaa atagttgagt
    18181 ttatagaagg tatagttgca gaagttggaa aagaaaaaat gcctgttgaa ttataccatg
    18241 caggagaaga aataactcaa ttggttcgcg aatttgcaac tgataagatg aaaaaagcag
    18301 ttcagacttt tgaaaagtta gagagaatgg aaaatatgga tagagttaaa gaagaaactt
    18361 tagctcactt tgaagaaact ttagaagatt ttgaagattt tgttggagat atagaagaag
    18421 tattacaaga tataataaaa gaagaagtaa gaaaacttat agtacatgaa aatgtaagac
    18481 cagataatag aaaacttgaa gaaataagac ctatatggtg tgaaactggc atgataccta
    18541 gagctcatgg ttcagccata tttactagag gacaaactca agtacttaat gttgctactt
    18601 taggtgcttt aggagatgtt caaaaactag atgggttaga tgaagaagaa aacaagagat
    18661 atatgcatca ttataatttc cctgcatata gtgttggaga agctagacca tcaagaggac
    18721 caggaagaag agaaatagga catggtgctt tagcagaaag agctttatta ccagtaatac
    18781 catctcaaga agaatttcct tatgctatca gattagtttc tgaagtatta agttcaaatg
    18841 gttctacatc tcaagcaagt gtatgtggct caacattatc tttacttgat gcaggtgtac
    18901 ctataaaaga tatggttgca ggtatagcaa tgggacttat aaagcatgat ggtaaggttg
    18961 cagtgctttc agatatacaa ggtatggaag accatttagg cgatatggac tttaaagtag
    19021 ctggaacaga gtatggtata acagcaatac aaatggatat aaaaattgat ggtatagata
    19081 aagaaattct acaacgtgct ttaaaacaag ctaaagaggg tagaatccat atactaggag
    19141 agatgagaaa aactatatct cagccaaaac cagaactttc accatatgca cctaagattg
    19201 ttaaaatgca aataaatcct gacaagataa aagatgtaat aggacctggt ggaaagataa
    19261 taactaagat aatagacgaa actggagtta agatagatat tgaacaaact ggggaagtat
    19321 ttatatcagg tattgagatt gatatgatta agaaagctca agaacttata aataatatag
    19381 tagttgaacc agaagtagga aaaacttata aaggtaaggt aagtagaata atgaactttg
    19441 gagcatttgt tgagatactt ccaggaaaag aaggattact tcatatatct catatagctc
    19501 atgaaagagt tgccaaagtt gaagatgtat tgaatatagg ggatgaagtt gaagttaaag
    19561 ttacagagat tgatgaaaaa ggaagagtta atttatcaag aaaagttctt ttaccaaaac
    19621 cagaacataa aaataaataa cttattatat aattaaaagc aaagtcttag gattttgctt
    19681 ttttaaatac aaaaaagtag ataattttaa aatgatagtt atggatggct tatagttctt
    19741 tttctgtcag gctttactct tataaagaag ctatttaaaa gctgattaat agagagcttt
    19801 attgatatat atagaatcac ttaagttata tttagtgcat aaacagagct tatttttaat
    19861 agtattaaat aagaaacaat ttgtacataa ttagggggag ttttaatgat aatgatggta
    19921 cttaataaga gtaagctcaa aaaaatcata atactaattt tagtagtgtt attagtatta
    19981 tcaggagggg taatggtatt aaagttattt aacaacaagc cagtatttaa tttatttaat
    20041 ggtgtggatt taccatatga aagaggtaca aaggataaaa gtggatatgt tgcattaacc
    20101 tgtaatatag accttggatg ggaaacagag tacgtagaat ccattttaga aacacttaaa
    20161 aaagaaaatg taaagattac atttaatgta actggaaaat gggcagaaaa gaataaggat
    20221 gaattattaa agataaaaaa acaaggacat gaaataggta atcatggata caaacattta
    20281 gattattcta cattatcata tgaagacaat tatgagcaaa tagagacctc taaaaagata
    20341 attgaagaaa ttataggtga aaaaactaaa ttttttcaag caccagcagg ttcttttggt
    20401 ccagaaacag taaaggctgc taaagcatta gggtatactt ctatcaaatg ggatgccgat
    20461 actatagatt ggaaatacaa agaccaacca gaggtaatca ttgatagaat gaagaaaaaa
    20521 gacattaaag atagtagtat aattttaatg catccaacca atgcaacgac aaaatgtata
    20581 gatgatatta tagctattgt aagagaaaaa ggactaaaac caggaaaact tagtgatgta
    20641 tttaagtaga caaagtataa atatttggtt aagattgtat aagttgtagt tgaattaatt
    20701 aaaagactga cttataatgt aatacatata aaaaataaat ataattaaaa catagtgttt
    20761 tcaataatcc aatatgaaac aaagaataaa ttgttaggag aatgtaatgt ataagacaaa
    20821 aatactggaa aatgggctta caataattgg tgaagaaata ccatatctaa agtcaataac
    20881 attaggtatt tggataaatg caggctctag gatagaagaa gcacaggtga gtggaacttc
    20941 tcattttata gagcatatga tgtttaaagg aaccaaaaac agaacatcaa aagagattgc
    21001 tagttcaata gataatttgg gtggtcaaat aaatgcattt acaagtaaag aatgtacatg
    21061 ttattatgta aaactaatag atgagcatat agatacagga attgatgtat tgagtgatat
    21121 gatactaaat tctaaatttg ataaaaatga tatagataag gaaagactta taatattgga
    21181 agaacttaag atgtatgaag actctccaga tgacttatct tatgatttat tagtggaaaa
    21241 tatatatgct aatgatggat taggaatgaa tataattggg acaaaagaat ctctgtacaa
    21301 tataactaga gaatccatgt tagaatactt aaataagtat tatattccaa ataacgctgt
    21361 tatttctata gctggaaact ttaactttga tgatatggtg gagaaaatca aatcaaaatt
    21421 tggacactgg gaaaagaaaa atttaagtat agacataagt gaggctaagt ttaatccttg
    21481 ctttatatca aaaaataaag atactgaaca ggtgaactta gctatgtgcc taaaaggtat
    21541 tccttttgaa aatgatgaag aagtctattc aatggcagtg gtaaataata ttttcggtgg
    21601 aagtataagc tcaaggcttt ttcagaaaat aagggaagaa aaaggtttag tatattcaat
    21661 atactcatca cagacattat ataggaaatg tggtgaatta ggtatatttg caagcatgag
    21721 tacagaaaat ttacaagatg tatataattt aataaagaaa gaaattgaaa atataagaga
    21781 aaactatctt acagaaaaag aaatatctga aagcaaggaa cagttaaaag gaaattatat
    21841 attggattta gaaagtacta gcagtagaat gatgtctact ggtaaatcta tgttacttag
    21901 taaaaaagta aaaactacag atgaaatact tgaatgtata aataatgtta atattaattc
    21961 aataaagaag gttgttgata aggtattcaa tatcgaaaat attggtacat gtatagttgg
    22021 aagagatgta gaaaagattc tacacttgga ttaatattga aaaaagtaaa tctctggagg
    22081 tataaaatat gaatttatct gaaatagcag gtaaggaaat agttaacttg gtcacaggag
    22141 aaagattggg ggtcattgga gaatgtgacc ttatactaga tgaaactaca ggcggtatat
    22201 tagcgcttct aattccaaaa gaaaaaggat tttttgccag aagaaaagat aagtcatttt
    22261 tagaagtacc atggagaaat gtaaaaaaaa taggtaatga catgataatt atagaatatg
    22321 agggatatat ttaggaggaa aaaatgagta ttttagtgca aaagtttggt gggacatcag
    22381 tagaatcata tgaaaaaatg aatgaagtat gtaagatagt aaaagcatac aaaaaaaatg
    22441 atgaagaatt acaattagta ttagtagttt cagcaatggg aagaaaagga gctccatatg
    22501 ctacagatac acttataaat ttgtgtagtg cagtaaatga tgagccaagt aaaagagaac
    22561 ttgatttaat aatgtcttgt ggagaaataa tatcaggaac aattcttgct aatttattga
    22621 atgcacaggg gatagattct gttttcttta ctggtcagca agcaggtata attacaagtg
    22681 atgaatattc taatgcaaag ataaagtata taaatcctaa aaaggtaaaa agagctttag
    22741 atgatggtaa ggttgtagtt atagctgggt tccagggtgt aacagatgat ggagaaataa
    22801 ctactcttgg aagaggcgga agcgatacat cagcagttgc tattggtaaa gctttagaat
    22861 gtgaaacagt tgaaatttac actgatgtgg atggtataat gactgcagac cctagagtag
    22921 aaccaaacgc taaagtattg agttttatag attatgagga agtattccaa atggctgaca
    22981 aaggtgcaaa agtaatacat ccaagagcag tacagttggc taaaagtgga aatataactt
    23041 tagccataaa gaatacaatg aatccaactt ttgaaggtac aaaaataggt tctttatgta
    23101 gacacttaga agacaatata gaatatgaac aagaaagaga ttttaaggtt gctgttgcaa
    23161 ataaagatag cgtagcacaa gttaagatta agtcagcaga agaagtgttt acagaagtat
    23221 taaatgagat agaaaagaaa cttataacta tagatatgat aaatttcttt atatcagaaa
    23281 aagcatttgt tgtagaagat gctgatataa aatcattaaa ggaaatacta gataaatttg
    23341 aattagatta tgaagtaaat agagactgtg ctaaggtaac attgatatgt tcaagaattg
    23401 ctgatgaaat gtctggtatc atgtcaaagg tagttagagg attatcaaaa gcaggggtat
    23461 ctcttttaca aacatcagac tcaaatatga ctatatcttg tttagttagt gaagaagata
    23521 tgcatacagc agtacatgct atacaccaac aattttattt aaaataaact gtataataca
    23581 attatttttt aatcaaattt aagaatttca tatatatctt aatatatttg atttatttgg
    23641 taaaactaaa ttaaatataa ttttgatagg agttttttta tgaaagatta tagagaagat
    23701 aatgaaagat ttgataaaga taattgtagt gaagaatcta gttgcgaaga gagagaatgt
    23761 ataaagcaat ttggtaccaa tgaaatgcca cctcaacctc ctaaagatat tcaatgtata
    23821 actataattg gagaaattga gggacatttt ataggaaatc ctcaaaaaaa agctacaaag
    23881 tacgagcata taattccaat gctatactca attgaggaaa gtaatgatgt taaaggagtg
    23941 ttagttgttt taaacactgt tggaggagat attgaagcag gtttagctat tgcagaactt
    24001 ttaaacagta catctaaaaa agtagttact cttgttctag gaggaagtca tagtataggt
    24061 gttcctcttg ctacagcagg agattactca tttatagcac caacagcaac catgattata
    24121 catccagtgc gaactactgg tcttgtgatt gggataaatg agacttttga gtattttaaa
    24181 aagatgcaag atagaataat tcaatttata ataagaacat caaatatcaa aaaagatgta
    24241 cttgaaaaac ttatgcatga aaaagatgaa cttgtaagtg atgttggtag tgtattaata
    24301 ggaaaagaag ctgtggatta tggtcttata gatgaagttg gaggactaaa agaagcactt
    24361 aaaaaattaa gagagcttat aaaagaatct gaggaagaaa aaaataatga ttaaaatttg
    24421 tttaaataat aaaatttatg atataatgat gtaataaagt aggagtgtta gtgttaaggc
    24481 attagcactt tttgctgtga aggggtaaaa cttgctaaaa ataaggagtg atattaatgg
    24541 ccacgaaaaa gaagaaaaaa aagaaggtaa ataccttaag ttttaatgca gaatatcata
    24601 atttaataac aatatttata ggtgtatttt tattatatag tttaaattct aattctatgg
    24661 gatggatacc tgtgttaatg caaaatcttt ttaagggact atttggaggg ctttcaatag
    24721 caataccatt tatagtaatt ataacaggac ttttaggatt ttttgatgga aatgaatata
    24781 tatataggct aagaaagact aaactatact acataattat tttatttata tttgtatttt
    24841 atggattatt aaatgcagga actcttcctg tagatagccc actaaaaggt aacatgtttg
    24901 atgatgttat gaaattgggt gtaagtggac aaggtagtgg tttgatagca acaactattg
    24961 cgtattatat gagtaaaata tttggtatag caggtggatg gttaataagt atcttcgcac
    25021 ttatactttc tgtaatgttt atatttaata tttctataaa agatttggtg tcaaatgcaa
    25081 aatcaaaatc taaagcatct aaagacagca atttaacgtt taaagataaa attgcaaata
    25141 tgaaaaaatc tgcaatagat atgatgactg atgaagtaga tgatactact ataaataaaa
    25201 aaccaggttt ctttaaaggg ttaatgtcaa aaggtcttaa taatgatgat gaagaagatg
    25261 aatattttga agaagaaaat agtgatggtg tagatgataa aactataaaa atagttggat
    25321 ttaataaggc agaagatgag tatttagaaa tattagaagg aactcaaagt atgccagagt
    25381 tagatgtttt aaaagaatta caaaaagcta ccaatgaaaa tccagttgta gatactaaac
    25441 cagaaaaaaa agttgatata gctaaaccta atttaaatat agaaaaaact caacctatga
    25501 gtattgtagc agagccagtt aatgaggatt attcaaacta taaaaagcct agcattgagc
    25561 ttttaaataa agtaaataag aagtctgatg aaaatggtaa aaagaaagtg ttgaaaaatg
    25621 cttctctttt agaaaagaca ctatcagatt ttggagtgga agcaaaaatt aatcaagtta
    25681 cagttggacc tactataaca agatatgaaa tacaaccaag tccaggagtc aaggtaagta
    25741 aaattgtaaa tttaactgat gatatagctt taagtttagc agctaaaagt ataagaattg
    25801 aagccccaat tcctggtaaa agtgcaattg gaatagaagt tccaaatgaa gaagctcaaa
    25861 tggttggtgt gagagaagtt ctagaaagtg aagaatttaa taattttaac tctcctcttg
    25921 caatgggact tggaaaagac gtagcaggaa aaattataat tggagatata ggtaagatgc
    25981 cacacttact aatagctggt tctacaggtt caggaaagag tgtgtgtgta aatacattaa
    26041 taagtagtat tttatataag gcaaatcctg atgaagtaaa attactatta atagacccaa
    26101 aagtagttga acttgcaaat tataatggaa taccacatct attgatacca gtagttactg
    26161 accctaaaaa agctgcaaat gctttaaatt gggcagttac agagatgaat agaaggtaca
    26221 aattatttgc agatgctcaa gtaaaagatg taacaagtta taatgagaaa gcggaagaaa
    26281 aacttcctaa aatagtaata ataatagatg agttagcaga tttaatgatg gcaagtgcaa
    26341 atgatgtaga agattatatt tgtagattag ctcaaatggc aagagctgct ggaatgcact
    26401 tgattgtagc aactcaaaga ccatctgttg acgttataac aggggtaata aaggctaata
    26461 taccatctag aatagcattt gcagtatcat ctcaaactga ttcaagaaca atccttgata
    26521 tgggtggtgc agagaagctt ctaggtaaag gtgatatgtt attctatcca cttggtgcag
    26581 caaaaccagt tagacttcaa ggggcattca tatcagagag tgagtcggaa aaagtaatcg
    26641 attttgtaaa aagtcaagtt aaagatggaa taaaatatga agaagatata attgaaacta
    26701 tatctaaggt taatacttca aagggtagtg atgaggatga gtttttgagt gaagcgatag
    26761 aatttgtcgt ggaaagtggt caagcatctg cctctatgct acagagaaga tttaaaatag
    26821 gattcaatag agcagctaga cttatagatt ctatggaaga aagaggaata attggagcaa
    26881 gtgagggaag taaaccgaga aaggtattaa taagcaaaca ggatttgcag aatttagaag
    26941 gtgaatagaa ttgtcagtta taaaaataga aaaagcactt aagctagata aagtaatatt
    27001 tgtagatgtg agaactgaag gagaatatga agaagaccat atattgaatg caatcaatat
    27061 gcctcttttt aaaaataatg aacataatga cgttggaaca atttataaga tgcagggaaa
    27121 acatgaagca atacaaaaag ggtttgacta tgtatcgtat aaattaaagg atatatattt
    27181 acaagcagca gaacttgcat taaattatga taatattgtc atatattgtg caagaggcgg
    27241 aatgagaagt ggcagtatag taaatttatt atcatcatta ggtgtaaatg tatatcaact
    27301 tgaaggtgga tataaggctt atagaaattt tgtattagag tatctaaggc atgtaatgga
    27361 tacaaaaaac tttgtatctg ttcatggatt gactggtaca ggaaagactg atttgttaca
    27421 tttattagat gaaaaaggaa tagatgtact ggatttagaa ggcatagcta aaaacagtgg
    27481 ttctaccttt ggttttataa cttttgataa aaagccacct tctcaaaaga actttgaaac
    27541 aaaactattt gaaagtatat ttttttcgaa agagaattat atatttgtcg agagtgaaag
    27601 caaacgagtt ggaagtgtta cagttccaaa tgaaattttc gaggcaatga cacgagaagg
    27661 acatcatata ttattagaat gtgatattga aaatagagtt gatagacttt gtagagatta
    27721 tatttatgat aaagatgaag gtaatatatt gattttaaaa gaatgtataa acaagtttag
    27781 aaaaagattg ggtaataaag tagtagatga ttatattgat ttattagaat caggtgaata
    27841 taaagaactt gtaaaaagat atcttttaga atactacgac ccactttata tgcattcagt
    27901 agaaaaatat aagtatagta agattataaa ttttaatgat acacaaaaag ctttagaaga
    27961 tgttatagac ttatatgaga gtatattgga aggagaaagt gaatgttaaa aatagcatta
    28021 gaatcattag gatgctcaaa aaatttagta gatgcagaaa taatgatggg aattttaaat
    28081 aacaaaggct ataaattaat tggagatttt gaagaagctg atgtaattat agtaaataca
    28141 tgtggattta tagagtcagc taaacaagaa tctatagata cgataataaa ctttgcagag
    28201 ttaaaaaaaa ctggcaattt aaagttgttg atagtaacag ggtgtctagc ccaaagatat
    28261 tctgaagaat taaaaactga aattcctgaa atagatgcta tagtaggaac aggaagctat
    28321 caaaatatag ataaaatact taaagaatta agtgaaatgc ataaaattgt aagccttaat
    28381 gatatagagt ttgtatttaa tgaagattta ccaagatata tatctacacc tagctatatg
    28441 gcctatttaa aaattggaga aggatgttcc aataattgta cttattgtat aataccaaaa
    28501 ctaagaggta aatatagaag tagaaaattt gaagatataa ttaaagaagc taagaaatta
    28561 gctgaatctg gagtaaaaga acttgtggtt atagcacagg atacgacaaa gtacggtttt
    28621 gatttatatg gaaaagaaag attatcagaa cttttagaag aacttgcaaa aatagatgga
    28681 tttaagtgga taagggttat gtattcatat ccagaatcta taacagaaga attgatacaa
    28741 gttataaaga aatatgataa tatatgtagt tactttgata tgccaataca gcatgcaagt
    28801 aacaatattt taaaattaat gaatagaaaa actactaaag aagatatatt aaataaaatc
    28861 aatctgataa gaagtaatat accagatgct atacttagaa ctacaataat agtagggttt
    28921 cctggtgaaa cagaagatga cttcaaacaa cttgtggact ttgttgaaga ggttaaattt
    28981 gatagacttg gtgcatttgc atattcaaga gaagaagata caccagcaga tagattgcca
    29041 aaccatatag atgaagaagt aaaaatacaa agaagagata ctttaatgat gattcaacaa
    29101 aaaatatctg aagaacttaa tgacaaaaag ataggaaaaa cttatgaggt gttaatagaa
    29161 gaacagatag aagataatgt atatacagga agaacacaag gtgatgcaga agaaattgac
    29221 agtatagtgt atgtaaaaag tgtagacaat cttgaagtag gagaatttgt aagtgttcaa
    29281 ataaatgatg caatggaata cgatttaatg ggagatgttt tatatgaact tgcctaacaa
    29341 gttaactttg tttagaatat ttttaatacc agtatttgtt ttaattatgt tgctcaatat
    29401 accaaataaa tttttaatag cttgtattat ttttataata gcttcaatta ctgatgcttt
    29461 agatggaaaa atagctagaa aatacaatct agtcactgac tttggaaaat ttatggaccc
    29521 attagcagat aagttattgg ttatttcagc acttacatgt atgatagaag accatcttgt
    29581 aagtagttgg atggtaatta taatagtggc tagagagctt acagttagca tattaagagc
    29641 tatagctgct gctgatggaa aagtaattgc tgctggaaac agtggaaaac ttaaaacgat
    29701 aactcaaatg gtatctatag tgtttttatt gttaggtgct cagtttgaaa atgtattgat
    29761 attaaatatt ggtgaaattc tcattcttat agctacacta cttacattat attctggatg
    29821 ggaatatcta tataaaaata aagagctttt tatgtcttct aagtaaaaaa ttatgcttta
    29881 ataaataaaa actaatgatt aataaataaa aaataacaac ttaaaatttt aaaaatgtaa
    29941 aaaagggtat actattatag tatacctctt ttcattttaa gaatattttc ttttgtgtat
    30001 ataattatta ataatggttt ataaataaaa tgtaatgaat aaaaatataa tcattagtag
    30061 cgtttttaaa ttgactaggt ttgatttatg ttatataatt aatatataac aagagaacaa
    30121 atgtttgtag ataaatgaag ggatggtata gagatgagtg tagatcaaga aaaattaaaa
    30181 gcgttgaatg aagctttagg taaaattgaa aaagattttg gtaaaggttc agtaatgaaa
    30241 ttgggagaag caacatctat gtctatagat gttatatcaa caggagcgat tggtttagac
    30301 atagctattg gtataggagg tctacctaga gggagaatag ttgaagtata tggtccagaa
    30361 tcttctggta agactactgt tgcgcttagt tgtgtagcat cagctcaaaa agatggagga
    30421 atagctgcat ttatagatgc agaacatgca cttgacccag tatatgcaaa agctttgggt
    30481 gtggatgttg ataacctaat aatatctcaa ccagatacag gtgaacaggc tttagagata
    30541 gcagaggcat tgataagaag tggagcgata gatataatag taatagactc agtagcagca
    30601 ttagttccaa aggctgaaat agatggagat atgggtgatt ctcatgtagg tttacaagct
    30661 agacttatgt cacaagcact tagaaagtta actggttcaa ttaaaaaatc aaattgtgtt
    30721 gctatattta taaaccagtt aagagagaaa gtaggaataa tgtttggaaa cccagaaact
    30781 actactggag gacgtgcact aaaattctat tcatcagtta gattggatgt tagaaaaata
    30841 gatacaataa aacaaggtga taaagttata ggtagtagaa ctagagttaa agttgttaaa
    30901 aacaaagtag caccaccatt taagcaagct gaatttgata taatgtatgg agaagggatt
    30961 tcaaaaattg gagacctttt agatatagct gctgatgtag atatagttaa aaaatcaggt
    31021 tcatggtata gttacaatga tactaaactt ggacaaggaa gagaaaatgt taaaaaattc
    31081 ttggaagata atttagattt aactactgaa atagatgaga aagttagagc attttacaat
    31141 ttaaatgaag aacatgaaga atcaggtact tcagtatcaa aagaaattgt agaagaataa
    31201 gtaaaaatac aaaaaaataa ctagtaatta aagacatgcc acgctcccta ttagcttgac
    31261 acaatgttga aaaaattata aaattaagtg aggataaaat atctaaattt aattttaatt
    31321 attaggaata ttttttaatc atagtgatga cattttggga ttattaacga aggaggtgga
    31381 tgtcatagat agtgtagtaa tgattgtaat aggtgcagct gttggtatta tagctggata
    31441 ttttgttaga aaaaatatat ctgaagcaaa aattggacaa gctgaaaact tggccaaaga
    31501 aataatagat aaagcacacc acgactcaga aacagtacaa aaagaaaaat tactagaagc
    31561 taaagaagag atccataagt ggagaacaga agctgaaaga gaaaacagag aaagaagaac
    31621 agaagtacaa aagtatgaaa aaagagtagt acaaaaagaa gaagtcttag atagaaaatt
    31681 acaaaattta gaatctaaag aagtaaacct aagtgaaaaa ttaaaggtag tggaaaagaa
    31741 agaagaagaa gttgaagtaa taaaaactca acagttagaa aaattagaaa gtatatcagg
    31801 aattacttct gataaggcca aagaaatcat cttaaccaat gcggagagag atgtaagacg
    31861 tgaaatgtct ataatgataa aagagataga aagtcaagct aaagaagaag ctgataagaa
    31921 atcaagagaa attataggat atgcaattca aaaatgtgca gcagatcacg tagccgaaac
    31981 tactgtaaca gtagtaagct tgcctaatga tgaaatgaaa ggtagaataa taggtaggga
    32041 aggtagaaat ataaggactt tagaaacctt aacaggtata gaccttataa tagatgacac
    32101 tccagaagcc gtaattttat caggatttga tccaataaga agagaaattg caagaattgc
    32161 tctagaaaaa ttaatagcag atggaagaat acatccagct agaattgaag aaatggttga
    32221 aaaggcgaga aaagaagttg acaatattat aaaagaatat ggtgaacaag ctgcatttga
    32281 aactagtgta catgggattc atccggaatt aataagatta ttaggtagac ttaactatag
    32341 aactagttat ggacaaaatg tacttaaaca ttctatagaa gttgctcata tagctggtat
    32401 aatggctgct gaaataggag cagatattag attggctaag agggcaggtt tattacatga
    32461 tataggtaag gctgtagacc atgaaatgga aggtacacat gttgaaatag gtatggattt
    32521 acttagacgt tataaagagt ctaaagaagt tatacatgcc atgagtactc atcatggaga
    32581 ttacgagcct caaacagtag aggctgtatt agttacagca gcagatgcaa tatcagctgc
    32641 tagacctggt gcgagaagag aaactttaga agcatatata aagagacttg aaaaattaga
    32701 agaaatagct aattcatatg aaggtgtaga aaaatcattt gctatacaag ctggtagaga
    32761 aataagaata atggtaaaac cagaagctat aaatgatgaa gaaatacatc tattggctag
    32821 agatatgact aagaaaatag aggatgaact tgaatatcct ggacaaataa aagtaagcat
    32881 aataagagaa acaagagcta tagagtatgc gaagtaaaag aaataatatc taaataataa
    32941 gaaaatattt ttagaaattt aaatttgcca caagatatga taatatatct tgtggctttt
    33001 aaattgttta agtttaattt tatataatgc aaaaataatc taagtgctaa aagtgctact
    33061 tacaaaagat taataaaata aagctttata tatgtattat ttatataatt atccaagttt
    33121 gtttgatata atgaagttgt aaaataaata tattactact tggaatgctt gtacttaaat
    33181 aagatgaaaa tacaatgacg ctaaagtgag gattagagag agggtacact atgaaaaaaa
    33241 atacactttt acatacaaat attatggttt gcattattat tactcttggt tttgtggcta
    33301 cctctataat tggatatcaa tctaatacca atatttttaa aaatgatatt gaacatgtat
    33361 ctacattggc tgctgaaggt atttattatc agatagataa actactttca gaacctataa
    33421 atgtatctct tacaatggca aatgacagtc tattaaaaaa ttttttagat ggtgagaaag
    33481 aacatttaaa tgatgaagaa tttatttata aattacaaga ttatctgaat gtgtatagaa
    33541 ataaatattc atatgattct gtttttttag tatcaactaa aaccaataat tattatcatt
    33601 ttaatggact tgatagaaat cttagcgcta ataactcaga gaatcaatgg tattatactt
    33661 tcttaaagaa tgatgatgaa tactcattaa atgttgataa tgatgaagca agtaacaata
    33721 gtattacagt ctttgtaaat tgtaagataa aagatgataa tggagcaact atgggaatta
    33781 taggtgttgg gttaaaggtt aattcattac aaatgttgct taaaggatat aatgacaaat
    33841 ttgatgttgt tgcacgctta attgatgata aagggtttgt acaattagca gtagataaaa
    33901 ccggtcatga gaatataaat ttttttgaaa attctagtag cgacttgtct aattcgaaac
    33961 aattaatatt aaacaacaaa aaggaacaaa agagtttttg gtattcatct gaaaaatcaa
    34021 aaagttatat tgtatgtcag tacataccta gtttaaaatg gcatcttgtt ctagaaaatg
    34081 attttacttt aatgattaaa cagcttcatc tgcaattttt taaaggcatt gcagttatag
    34141 ttttaattat aatatttgta ctttttacta ttacacatgt gcttaaaaaa tacaaaaacc
    34201 aaataataaa gcttagtgtt tcacagaaat tggaatatca aaaattgttg agcaaatcta
    34261 ctgaaggtct ttatgaaaat atttttgaaa ttgatattac taacaataaa gctggttgtg
    34321 aagatgagaa taggtatttt catgaatttg gattagggct taatacccca tatgatgaag
    34381 ccttacatac aatagctaaa aaccaaatca aagaagaata tgcacaaggt tatttagata
    34441 catttttacc tgaaaatgta attaaacgtt ataatagcgg aattaataat atcagctatg
    34501 attttttaat taaaaaagat gaagaaagtt atcattggat tagaattaat gcacgaattt
    34561 tcttttggac atcagatgaa tctgttcgta tgatatctta tcgtaaaaat atagatgaag
    34621 aaaaaaatag agaattacta cttctcgaac gttcacaacg tgattctctt acagggcttt
    34681 acaataaagc tacaacagag gaatttattc gttcattttt aaaaaatgaa agtaatcaag
    34741 atttagaaca cgtttttcta attttcgata ttgataattt taaaaatgta aatgataaat
    34801 atggtcatgt atttggagac tttgttataa gtgaatttgc cttagaatta aaatctcaat
    34861 ttcgtgagga cgatattgtg ggacgtattg gtggagatga gtttgtagta ttaataaaag
    34921 actttaataa taataactat gtgcttatag acaaattaga gcgtttttgt tctaagatta
    34981 atcagaaaaa atttgttgag caagcagaat tttctattgc atgtagtatt ggtgtagcaa
    35041 tgtatcctga acatggatat gtatactctg aactatatga gaaagcagac caagcacttt
    35101 actatgcaaa aagccatggg aaaaattctt atcatatatt ttcagaggat tctattgata
    35161 gtatttcatt tcatatagac tctagagata taaaagtatt attagaaatg gctactgatg
    35221 gtgttgtcaa atttgcttgt atggaaaact taaaattact ttatttcaac caaaaaatgc
    35281 ttgaatttac tggatattca gctcaaactg ttacttctat aggttttgat attttttcgc
    35341 ctgtattaca agacgactta caaatggtac tagatacatt aagagaagca atttctaaaa
    35401 gagagacttt tgaaatagtt tttcgttttt gtcatacaga tggtcatatt tttcctgtaa
    35461 ggatgcaagg taagtttgta agtgaattat atcaaaatcg ttatcctgtt ttttatgcga
    35521 tttatacaga catatcaagt atcaatactt tactagaaaa taaatagtaa atactacagt
    35581 aaaatctaaa aataggatat aatagtatta atataagaag attataaata aaacctgcta
    35641 atgaaaatag attattt
//
LOCUS       DAELNY010000021        32951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.021, whole genome shotgun sequence.
ACCESSION   DAELNY010000021 DAELNY010000000
VERSION     DAELNY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.021"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..517
                     /locus_tag="KPE55_000901"
     CDS             <1..517
                     /locus_tag="KPE55_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0491997.1"
                     /translation="LIDIIDNYNFSREPVNTKNLGKKMIDNGNLSNLKELLLDNISFL
                     KQSTGTSREMDFVSSSYNIGLYIKLCWIVDGGEEYKMKNYMESNLIYSRVGKDLFNKM
                     TDESRRKNFSMKVIQLLKSNDRTQLFQTIMHVLVSNGILIGEGFVEGLMQSSELELNT
                     NVGLFIQEIMK"
     gene            537..1550
                     /locus_tag="KPE55_000902"
     CDS             537..1550
                     /locus_tag="KPE55_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG0491998.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            1566..2363
                     /gene="cas5"
                     /locus_tag="KPE55_000903"
     CDS             1566..2363
                     /gene="cas5"
                     /locus_tag="KPE55_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG0491999.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            2390..4798
                     /locus_tag="KPE55_000904"
     CDS             2390..4798
                     /locus_tag="KPE55_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0492000.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINNILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNKNVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDIDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            5239..6186
                     /gene="prmA"
                     /locus_tag="KPE55_000905"
     CDS             5239..6186
                     /gene="prmA"
                     /locus_tag="KPE55_000905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG0492001.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            6205..6963
                     /locus_tag="KPE55_000906"
     CDS             6205..6963
                     /locus_tag="KPE55_000906"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG0492002.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            6965..8263
                     /gene="mtaB"
                     /locus_tag="KPE55_000907"
     CDS             6965..8263
                     /gene="mtaB"
                     /locus_tag="KPE55_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG0492003.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            8306..8656
                     /locus_tag="KPE55_000908"
     CDS             8306..8656
                     /locus_tag="KPE55_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG0492004.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            8819..8998
                     /locus_tag="KPE55_000909"
     CDS             8819..8998
                     /locus_tag="KPE55_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG0492005.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            9028..9471
                     /locus_tag="KPE55_000910"
     CDS             9028..9471
                     /locus_tag="KPE55_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG0492006.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            9582..10079
                     /locus_tag="KPE55_000911"
     CDS             9582..10079
                     /locus_tag="KPE55_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG0492007.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            10272..10835
                     /gene="raiA"
                     /locus_tag="KPE55_000912"
     CDS             10272..10835
                     /gene="raiA"
                     /locus_tag="KPE55_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0492008.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            11048..11284
                     /locus_tag="KPE55_000913"
     CDS             11048..11284
                     /locus_tag="KPE55_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG0492009.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            11297..12640
                     /gene="yqfD"
                     /locus_tag="KPE55_000914"
     CDS             11297..12640
                     /gene="yqfD"
                     /locus_tag="KPE55_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG0492010.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            12579..13694
                     /locus_tag="KPE55_000915"
     CDS             12579..13694
                     /locus_tag="KPE55_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG0492011.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            13751..14212
                     /gene="ybeY"
                     /locus_tag="KPE55_000916"
     CDS             13751..14212
                     /gene="ybeY"
                     /locus_tag="KPE55_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG0492012.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            14218..14937
                     /locus_tag="KPE55_000917"
     CDS             14218..14937
                     /locus_tag="KPE55_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG0492013.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            15056..15457
                     /locus_tag="KPE55_000918"
     CDS             15056..15457
                     /locus_tag="KPE55_000918"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0492014.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            15521..16414
                     /gene="era"
                     /locus_tag="KPE55_000919"
     CDS             15521..16414
                     /gene="era"
                     /locus_tag="KPE55_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG0492015.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            16426..17805
                     /gene="mgtE"
                     /locus_tag="KPE55_000920"
     CDS             16426..17805
                     /gene="mgtE"
                     /locus_tag="KPE55_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG0492016.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            17878..18033
                     /locus_tag="KPE55_000921"
     CDS             17878..18033
                     /locus_tag="KPE55_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG0492017.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            17999..18772
                     /gene="recO"
                     /locus_tag="KPE55_000922"
     CDS             17999..18772
                     /gene="recO"
                     /locus_tag="KPE55_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG0492018.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            18765..19364
                     /locus_tag="KPE55_000923"
     CDS             18765..19364
                     /locus_tag="KPE55_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG0492019.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            19524..20402
                     /gene="glyQ"
                     /locus_tag="KPE55_000924"
     CDS             19524..20402
                     /gene="glyQ"
                     /locus_tag="KPE55_000924"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG0492020.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            20402..22468
                     /locus_tag="KPE55_000925"
     CDS             20402..22468
                     /locus_tag="KPE55_000925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG0492021.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(22634..24181)
                     /locus_tag="KPE55_000926"
     CDS             complement(22634..24181)
                     /locus_tag="KPE55_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG0492022.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DTFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            24461..25228
                     /locus_tag="KPE55_000927"
     CDS             24461..25228
                     /locus_tag="KPE55_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0492023.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            25511..25708
                     /locus_tag="KPE55_000928"
     CDS             25511..25708
                     /locus_tag="KPE55_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0492024.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            25737..26813
                     /gene="vorB"
                     /locus_tag="KPE55_000929"
     CDS             25737..26813
                     /gene="vorB"
                     /locus_tag="KPE55_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0492025.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            26816..27535
                     /locus_tag="KPE55_000930"
     CDS             26816..27535
                     /locus_tag="KPE55_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0492026.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            27548..28087
                     /locus_tag="KPE55_000931"
     CDS             27548..28087
                     /locus_tag="KPE55_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0492027.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            28244..29302
                     /gene="buk"
                     /locus_tag="KPE55_000932"
     CDS             28244..29302
                     /gene="buk"
                     /locus_tag="KPE55_000932"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0492028.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKDGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            29320..30213
                     /locus_tag="KPE55_000933"
     CDS             29320..30213
                     /locus_tag="KPE55_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0492029.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            30243..31451
                     /locus_tag="KPE55_000934"
     CDS             30243..31451
                     /locus_tag="KPE55_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0492030.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            31475..32734
                     /locus_tag="KPE55_000935"
     CDS             31475..32734
                     /locus_tag="KPE55_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0492031.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        13239 a         3403 c         6130 g        10179 t
ORIGIN      
        1 acttatagat attatagata attataattt ctcaagggag ccagtaaaca ctaaaaattt
       61 aggtaaaaaa atgattgata atggaaattt atcaaattta aaagaattat tacttgataa
      121 tataagtttt ttaaagcaat caactggaac ttctcgagaa atggattttg tatctagctc
      181 atataatatc ggtttatata taaaattgtg ttggattgta gatggagggg aggagtataa
      241 aatgaaaaac tatatggaaa gtaatttaat atattcaaga gtaggtaaag acttatttaa
      301 taaaatgaca gatgaatcca gaagaaaaaa tttttctatg aaagtgattc aactacttaa
      361 gtctaatgat agaactcagc tatttcaaac tataatgcat gtgttagttt ctaatggaat
      421 cttaatagga gaagggtttg tagaaggtct aatgcagagt tctgaattag aattaaatac
      481 gaatgtaggt ctatttattc aagaaatcat gaaataaaaa agggggaaaa tttaaaatga
      541 atgaaaaaag agtaaaagga gtaacattaa ctatactgac agaaagccca gttccacttt
      601 catatgacca agggtatgga aattatacac caataaaaaa agaacaatat agagaaaaaa
      661 tacatgcaaa aactagtata gccacaataa catatgattt aagaagaatg ctccaccaag
      721 aatatggttg gaatttaagt aatattgttt ttggaaaaaa aggaaatata tatccatcta
      781 ttaagaaaaa tgtaggatgt gtagatgaaa atggattaga gactgatgta tttggttatt
      841 tgataccact taaagataaa ggttctataa gtaaagcttc tccacttaga ataatacctt
      901 ttaggtcatt aaatccatat ataggttcaa cacagctaat aacaaataga ggttttttaa
      961 gtagcgaatt tggtagaaaa tattatgatg agaaggaaga aaatgaagta cctagagatg
     1021 aaaactttcc aacaacacaa gcattagcaa tagaagagac tttatcagat tactacgttt
     1081 atacaataac attggagctt gatagaatag gagtagtaga agtagaagat gggaagttac
     1141 ttttaccaga agaaagaaaa tttatgagta aggaattaag agaaaaggca gtaaaagata
     1201 tattagatgc aattactgtg tttacaagaa acataaaaca ttcatcagta ctattaaaac
     1261 cacttgcagt aataggtgga gcttttgata aagttgtacc atttttttgg gatgatgtag
     1321 attataacgc tgatagtggt gagataaatt tagagggagt catagaaact atagaaagtt
     1381 atagccttaa agaaagtaac acaatacttg ctatcaatga cagattaaaa atttctaata
     1441 aaaaggaatt aaataagttt aatttaagta aataccctgt aaaagagatt aaaaatctag
     1501 cagatagatt ggaaattgga gaagataata tgtggtattt aaaagagtag gaggtttttt
     1561 gaattatgaa agtattacaa tgtaaactta agcaaccaac tgctcactat agagacccaa
     1621 aagtatttca aaatgaatat ataagtactt taaatttacc atctaagacc acaataatgg
     1681 gaatgataac gtatttatgt gatagaagat taaactctga tatagatata ggaataattg
     1741 gaacacatca tcatagagaa ctagaatttt caagaggaga gaacattgat ttttggaatg
     1801 aatatacaaa tatgagaaaa ggcaaagata aagaaaagtt tttgcttcaa ggaaactact
     1861 atgattatta taaagaacat aaagctcaaa atagcatatt aaactatgaa gttcttaaag
     1921 aagtagaact gactatattt atttcttgca aggatgatga agaacttgaa tttattaaaa
     1981 aaaagcttga aagtccatgt aaatatgcaa atttaggaag aaaagaagat tttgtaatac
     2041 caagtgaaaa agggtgtttt gtgaaagaag ttgagttaaa agaggttata cctatgaata
     2101 ctcgtgatgc tataaaagaa aatataaaat taaagaatac atatgtaaga gttgacttaa
     2161 gagataaaga gaatgtcgag actataataa atcaaggtgt attgattgca ttacctcata
     2221 aatataaaga tttagaagca aatagagatg atagagttta tgagttttgt cattatatat
     2281 atgttgataa tgatggtata tatcctaaaa atattaaggt aaatgtatgt attgatacaa
     2341 aagaagtttt tacatggctt taaattattt tgaaagatag gtgtttatca tgaaaaatat
     2401 catgcaaaaa gaaatattag ctaagtcata caatttaaga actaatcaac aagagcaaac
     2461 attagttgaa catattaagg atttatttga agtattagaa tctatattag aactaaatct
     2521 atattcagat aaagatgtag agatattgaa aatatgttgt gctttgcacg atttaggaaa
     2581 aattaattca attatgcaac aaaaaatgga gattaataat aaaatatcgt atagttgctc
     2641 agaagaagag aggaaaaagt tagagtctga caaaaaatct ttaaaaaaga tagcaagaca
     2701 taatatattt tctggagcat ttttaaaaga tatattagaa aaaatgaact tgtcagaaga
     2761 agataagtta tatatatata aaagtataat gttacatcat ggaaactatg aagattatat
     2821 gaggttaagt attagtaaag ttcaagaaga aatttatgag tatattgaaa aaggcatttt
     2881 agaaaaagaa gagtttaatc tgaaagatat agaaagttat ataaataaca ttttaaatgt
     2941 agactttaaa tttggtgaag atgtattgga ttatgattac atagataaat taagtgaaag
     3001 tatgtttata gacagtgatt ataacaatgg tcaaatagat gattcagttt taaattatag
     3061 aaagtttaag tatattttat ataaagggat gctgaactta atagaccata gtgcatcaag
     3121 tagacagaaa ggtataaaat tttataacga ttttactgat gaagaaattg ataatatgat
     3181 tttaaatgag atatataaga gtcagggtaa tttagaaaaa aataatatag agttcaatac
     3241 tatacaaaaa aggttgagag aactttctgg aagaaatgtt ctgactgttg catttacagg
     3301 ttcaggaaaa acagctgcag attatagaaa gacttttaaa agaaaattct ttttagtgcc
     3361 aaataaaata tcagcagaaa gtttttatag aaaaaatatt tttcaaaata aaaatgattt
     3421 agatacaaga agtagtaatg attacattgg attacttcat ggagatataa acttatattc
     3481 agagaatgaa gataatggag aacatgattt tgtattgaca ttaagggata tagatttgag
     3541 tataaacttt tgtaaacctt gtgtaattgc aacagttgac cagttattac ttagtatgtt
     3601 taaatttccg ggatatgaaa aaatattcgc agccgtaaaa aatgcatcta taactgttga
     3661 tgaagtacat ttattggaac caaagatgtt tttgattttg atatatttta tgcagtttgc
     3721 atgcaagtac ttagatgttg attttcactt aatgacagct acacttccaa aatcatataa
     3781 agagcaaatg ataaacaagg gaattatttt tcaagaagaa agtaataaaa atgttactga
     3841 taaaggagaa atagttttta ttgagagcaa caaggaagaa gagatttgtg aaggtaaaga
     3901 tgtaaaggtt tcttttataa aggaaaaaga aatcaagtca atagttgaag atgctttaga
     3961 aaataaacaa aaaattctta taataaagaa tactattgat tcagtcaata gaacatatga
     4021 gtttttaaaa gaaaatttat ctgataagta tggtggtgta gatatagatg ttttacatag
     4081 tagatttaag ttcaaagata aaaaagagaa atatagtaaa atattaaatg gagagggtga
     4141 tatttggata tcaactcaat cagtagaaat atccttggat ttggatttca atataataat
     4201 aagtgattta gcaactatgg atagcttgat acaaagaatg ggtagatgca atagaaataa
     4261 taaatatgaa tatggaaact tttatattct acctagtgaa gataaaattt atgataacaa
     4321 acttaagaac acaactaaaa acatcttaaa ggacatatta aaaactaact ctatttttac
     4381 aatgggaatt agaaaaacta ttttggatga ttactatgat aatagtgtag tcaaaaaata
     4441 ttttgaagag aattttataa gttgtgataa agaaattaag aatatttatg gtataaataa
     4501 agaaattttt gatggattag atttaatttt taattttgaa ccttacaaaa atattgttga
     4561 tagtaagaag gaagcagcta aaatttttag agatattgat gttagttaca aaattattct
     4621 ggaagaagat ttttataagg aagataggga gttacaacaa gattcgattc aagtatcagg
     4681 gtttatattt aatagattat attattatgg attaataagt aaagtagaag gatacatggt
     4741 actaaaatct agtagtaagt ttgagtataa tagtactgta ggcttaattt tgaagtgagc
     4801 atatgtttaa atataatata cctttagcca ttgaaaatac aaaaagtact actgtaagtc
     4861 cactgtaaaa actatttttt aaaaggaaaa ataagttgtg agatagagtg tttacaagtg
     4921 tatatagata ataaatttga ggtttaagat taactaagtg ggtttcaggt acatcgcgaa
     4981 aataattata agttggagat tttgtatgtt taagattaac taagtggatt tcaaatattg
     5041 atatagtaga tataatagaa gttttattat taggtttaaa attaactaat cggattttta
     5101 aatattctga ctattatata aggatattat tagcttgaat ttaaaaccaa taatataaaa
     5161 caacaaaata ttatatttat gatataataa aagttatgga aaaacaggaa tattactata
     5221 gagaagagag gtaaacttat gaataattgg atagaagtaa ctataaaaac aacaacagaa
     5281 gcagttgaac ctataacgaa tatactatat gaacaaggag ctggaggagc tgtaatagaa
     5341 gacccaaaag actttttatt ccaaaagaaa aatgaactag actgggatta tgttgaagaa
     5401 gaagtattta aaaagaatga agaagatgat gttcttataa aaacttatgt ttctgaagaa
     5461 aaaaatgtga tggaatttgt cgaaataata aagcaaaaag ttttaggatt aaaagatttt
     5521 ggtatagata taggtgaagg aagtgtatca ttggaccaag taaatgaagc tgattgggca
     5581 aatgcatgga aagcatacta caaaccaaca aaagtaggac aaagggtagt agtaaaacct
     5641 acatgggaag attatgctat gcaagagggg gatttaatca tagagcttga cccaggaatg
     5701 gcatttggta cagggacaca tgagactact agtatgtgca ttagagaatt agaaaagtat
     5761 gtgaataaag attctaaagt atttgatatt ggctgtggta gtggaatcct tgcaatagca
     5821 gcagctaaat taggagcaaa agaagttgta gctgtagatt tagatgaagt ggctgtaaaa
     5881 gttgcaaaag aaaatgttct tgaaaataaa gttgaaaaaa gtgtatctgt aatgcatggt
     5941 aatttaactg atgtaataaa agataaagcg gatgtaatag ttgcaaatat aatagctgat
     6001 ataataaaga tacttgcaaa agatgttcaa aactttatga aagaagatgc tatattcata
     6061 tcttcaggta taattttgga taaagtagaa gaagttaaag aaagcttaat tgaaaatgga
     6121 tttgaaatag ttgaagttca aaaattaggt gaatggtcag caatagtttc taagcttaaa
     6181 aaataaggga tatggtgacg tataatggat agattttttg ttgaaaaaaa taatataaat
     6241 ctacaagata aaacttgcac tatagaaggt gaagatgtaa aacatatttc aaaagtttta
     6301 agatgcaaat taggtgagaa acttgaaata tgtgacaaga ataataatga gtatatttgt
     6361 gagataatga atatagataa gagtatagtt aatttagaaa ttttagaaaa agttgatata
     6421 aatagagaat cagaacttaa agtaaggcta taccagggac ttccaaaagc tccaaaaatg
     6481 gaaatgatac ttcaaaaatt aactgaagtg ggtgtagaag aaataatatt agttcaaact
     6541 aagagaagtg ttgttaaagt tgatgacaaa aaagaagata aaaaatttga acgttgggaa
     6601 agaataattt atgaagcagc aaagcaaagc aaaagaggta agataccaaa attaagaggt
     6661 gttttaagtt ttaaagaagc tttagaagat atgaaaaaaa ataatgtaaa tatttgccct
     6721 tatgaaaatg aaagaacagt ttcaataaaa catgcactaa aaaaatgtga ttctaatata
     6781 gatagtgttg gaatttttat aggtccagaa ggtggatttt ctgaagaaga aatagaacaa
     6841 atacaaaaaa ataattgtaa tgttgtatca ttggggccta gaatattaag aactgagaca
     6901 gcttctgttg tagcatcaac aatagctttg tatgaactaa gtgatttagg aggagaaaaa
     6961 taaattgaaa aaagtagctt tttatacact tgggtgtaaa gttaatcaat atgaaacaga
     7021 agctatgcta gaactatttg agaaagatgg ttatgaacaa gtcaatagtg aagagtatgc
     7081 tgatgtttat gttataaata catgtacagt tactcatatg agtgatagaa aatctcgtca
     7141 atatataaga cgagttaaaa agaagaatcc agatgcaata atagccgttg taggatgtta
     7201 ttctcaggta tctccggaag aaattttgga tatagaagaa gtaaatttag tcatgggtac
     7261 aaatgataga agaaagattg ttgaagaaat taaaaagata aattcaagta agaaagttag
     7321 tacagttgac gacataatga aagttaaagc ttttgaagaa atagaaatca gccaaactaa
     7381 tggaaagact agagcattta tgaaaataca agatggatgt gacagatttt gtacttattg
     7441 tatcattcct tatgcccgag gacgcgttag aagtagagat attgatagta tagtcgatga
     7501 agtgaaaaaa ttggctaata atggctataa agaggttgta ttaacaggaa tacatgttgc
     7561 ctcttatgga aaagatttaa aggataggga tattaaactt cttgatgtaa taaaacaaat
     7621 aaaccagata gaaaaaatag aaagaattag gcttagttct gttgaaccaa ttttatttac
     7681 agatgaattt gtaaatgaag ttttaaagat ggataaagtt tgtcctcatt accatttatc
     7741 acttcaaagt ggatgtgatg aaactctaaa aagaatgaat agacgataca caactttaga
     7801 gtacaaaact attgtagata gattgagaag taaaatgcca gatgtggcaa taactacaga
     7861 tgtaatagta ggttttcctg gagagacaaa tgaagagttt aaaaaaacgt acgagttttt
     7921 aaaagaaata gagctttcac aaatgcatat ttttaaatat tcaccaagaa aaggaactcc
     7981 agctgcaact atggaaaatc aagttgaccc tcaaatgaaa cactttagaa gtgaacaact
     8041 tttaaattta agtaaagtaa attttaataa atttgcaact aaatttatcg gccgtgagtt
     8101 ggatgttttg tttgaacaaa atatagaagg caataaatat gagggattaa cttcaaatta
     8161 tataagagtt gttgttgaaa gtgacaaaaa tatacaaggt cagatattaa aagtgaaaat
     8221 aaatgatgta aaagatgaat atgtagaagg aattttgcta taacatgtag aaattataat
     8281 atttataaga taggaggtgg aaagaatgga ttgtattttt tgtaaaatag cgaacggaga
     8341 aataccttca actaaagttt atgaagatga tagagtctta gcttttaatg acttgaaccc
     8401 agttgctcca tatcatatat tagttgtacc taaaaaacat tatgatagtt taattgatat
     8461 tcctgataaa gagatggaca ttgtgtcaca tattcatgtt gttattaata aaatagctaa
     8521 agagaaaggt tttgaccaaa ctggattcag agtcataaat aattgtggaa gtgatggagg
     8581 gcaagaagtt aaacatttgc actatcatat tttagctggt aaaaagcttc ctaattacga
     8641 agcgggacaa aactaaacta aattattgaa aaattgagtt ccttcttgat aaaaatactt
     8701 gataaaagta aaaatttagt ttataataaa caaagtgtta ggttgtaatg catattatgc
     8761 aaaaagaaca tagttacagt cccagcataa ttgcgctatt ggagggaggg aaaaagaaat
     8821 gtcagaagta agagttagag agaatgaaac attagacagc gctttaagaa gattcaagcg
     8881 tcaatgtgca atgtctggca tcatgtcaga agtgagaaaa agagagcact atgataagcc
     8941 aagcgttaag cgtaagaaaa aagctgaagc agcaagaaga aaaaacgcta agaaatagta
     9001 gtaaataaga ataaagaggt gaaggggatg tcccttaagc aaaagttaca agaggatttg
     9061 aaatcttcga tgaaaaacaa agatacagtg agaaaatctg tagtaacttt aataagagct
     9121 tctataaagc agtatgaggt tgataatagg gtcgagcttg atgaagatgg aattatcgat
     9181 gtaatagcaa aacaattaaa gcaacgtaga gatgcattag ttgaatttga aaaagctgga
     9241 agagaagact taataaaaga aacagaaggt gaaattgaag ttttaaaaga gtacctacct
     9301 caacaattaa gcgaagaaga gttagaagaa attgtaaaat ctactatatc tgaagtaggg
     9361 gcaacttcta tgaaggatat gggaaaaata atgtcagtta tccaaccaaa agttaaagga
     9421 agagctgacg gaaaacttat aaataagttg gtaaaacaaa acttacaata gattgatata
     9481 gcctagagag aaatctctag gctttttata gtttttggga ataacgagtc atatttgagc
     9541 atatctataa tcaaagaaaa ttttgaaaga aggggaaaaa tttgattata tcagtaaaag
     9601 agattaggaa ttttttgata aacatattta taccactaat aataggttac ttaagtagca
     9661 tcttaacaat gattatcagt ggaacagata tttcaaccta ttatttacaa ctcacaaagc
     9721 caagctttgc accaccagct tttatctttc ctatagtttg gactatattg tacatattga
     9781 tgggtatatc ttcataccta atattaagaa aaggttacaa cttacctaaa gtaagagatg
     9841 caatttttta ttatggatta caacttgtat taaactttat ttggagtata ctattttttg
     9901 gatttggact tagatttaca gctttgatag atataataat aatgattttg gtttctataa
     9961 taatgatttc gaagttttct aaaatagata agagatctgg atacataaat ttgatttatt
    10021 taatgtggct tgtttatgca ggatttttga actattttat atggtttatt aataaataga
    10081 atgtaaagat tttgtaaatt accctgtact tttaataata tcataacaaa cgaaaaataa
    10141 atagactatt aaaaataatc attttcatat acaataatat taatttaaat acaataaatt
    10201 tataaaaatt gtatttaaag attaacatat taaagtatta gaattggaaa ggcttgaagg
    10261 gagaggtttt tatgcaaata atagtatctg ggagacaaat gaaattaact gacggaataa
    10321 aaggatacgt agatggaaaa ttaagtaggt tagaaaaata ccttgatcct gaatcagaag
    10381 tgaaagtaac agtaagtgcg aaaaaggata gacaaaaagt ggaggttaca ataattccta
    10441 ttaacggtca aataataaga gcagaagatg tagaagatga cttatatgca gcaattgata
    10501 ttgtttgtga caaattaagt agacaagttg tcaaatataa aactaaagtt aaagacaaag
    10561 ttcaaaataa taagagcata agatttgaaa atcttgactt tatagataat agcagtgaat
    10621 ttgatgatta tgattatgat gatgaagaag atgaaaatat agtaatagaa agaagaaaga
    10681 aatttaatgt aaaacctatg agttctgagg aggcgatact tcaaatggaa ctagtaggac
    10741 acaatttcta tatgtttaga aaccaagata attttgaaat aaacatagtg tataagcgta
    10801 aagctggtgg atatggttta atagaacaag attaataaaa attgtatacg tttagatttt
    10861 atataagaaa aaataaaaag gtaatcatgt tgatttttta tcaatatgat tacctttttc
    10921 ttgtttaaat aatatattag atattaggta tttattatat aaaaatttta aaactaatct
    10981 gttacaacat caataaagtg cataaatacc tatttcaagc ataaaattaa ttaagggggt
    11041 aatgcttatg ttagaaatat ccactgattt acaagtaaat caaccagtta ttactgttac
    11101 aagtaatacg tttattagta tagaaaatta tctatcaata ttagaatatg aagttgattt
    11161 aataagaata aagactaaag ttaaaacaat taaaatatct ggggataaac tttcacttaa
    11221 gtatattact gatagtgaga taggtataaa agggattatc tacaatgttg agtatgttga
    11281 ttaggaggag atacacttga taagttttat aaggggatac tatgttatag ttgtggaagg
    11341 agtaggactc gaacaatttt taaatcatct tataagaaat gggataagtg tttacaatgt
    11401 aactagaata aaaaatacta aaatggagtt tcatatagat agacaagata taaaagactt
    11461 taagaatgtt tatagaggaa gtaagtttga tataaaggta aagcaaaaaa ctggggtccc
    11521 ttttattatc aaaagagttt ataagcacaa aggtatgtgg atttgtgctc ttgtttcttt
    11581 atttttattg atgtctacat cacagtttgt gacagatgta tatatacaat ctcctgaagg
    11641 tataaaaaaa gaagctctta gaaatgagct ttataaggtt ggagtaagac caggagtcta
    11701 taagaagagt attgatagaa aagaagttag agaccatatg atgtcaaaat ttaatgatgt
    11761 ggcatattta tctatcaatg taaaaggaac aaatatattt gttactgtta caaaaaaggc
    11821 agaatcccta aaatctacag accaatcaaa ttattgtaat gtaattgctt taaaaaatgg
    11881 tataatagaa aaggtaatcc ctagaagtgg taaatcagtt attaaatctg gagacatagt
    11941 ccaaaaggga gatgtcttat taaatggagc taataccaag tcaataccag aagtatgggc
    12001 aagtactttt tatgagtcca caaaaaaggc aagttatgta gatactgtaa ataaaaaaac
    12061 tggagaaaag aaaaatatat atacactaag tttttatgat aaagagttta caatgagaaa
    12121 aaatataaaa tataaagatt atgttgttga aaataaagaa aaaaaactat caataggaaa
    12181 ctatactttt ccaataaaga taaaaacgag tactttttat gaaacaaaaa aggttgaagt
    12241 aaaaaggaat aaagaagagt taaaaaaaga attatctgaa aaagctttaa aagagttaga
    12301 atacataatc ccagcttcgg ctagaataat agatgtaaaa cataacttta aggttaataa
    12361 aaatatgtta gaatatttaa taacagttca aactagtgaa aatattgcta aaatttatcc
    12421 tcttagtaag tcagaagcag aaaggtttat aaaggaagaa agtaaaccag atgaaggtga
    12481 agaggaagtt ccttccaatc cagaaaaaag accattaaat gatataagaa atgagtttga
    12541 tgaggataat aaggataata aagatcaaaa caattctgat gaaaataata gtaatcaaaa
    12601 tagtaataat agtcaaaata ataatagcaa taacaactaa taaaattaaa cttaaaatct
    12661 taggaggact aattcaattg ataatacaaa agaaatttac aatagcagat ggtaactttg
    12721 aaagagagtt atttggcaac tttgatgaaa atgtaaaatt aatagagaag actttaaata
    12781 tagatgtaat acttagagaa ggcaatataa ttttaattgg agaagaaaaa aatgtagatt
    12841 ctgcattaaa acttatgaat gaattacacc aaactgtatc aaatggtaag catttagata
    12901 agcagagtat ttcttattca ttatctcttt tgcttgaagg aagtgaacaa aaaataaaag
    12961 aacttgaggg gacaatcgtc ataactcaaa ggggaaaagc tgttcaacct aaaactttag
    13021 gtcaaaaaga atatataaaa cttatagaaa ataatgatat aacttttggt gttggacctg
    13081 ctggaacagg aaagacttat cttgcggttg ctatggcagt aaaagctttt aaaagagatg
    13141 aagttagtag aattatactt acaagaccag ctgtggaagc aggagagagt ctaggttttt
    13201 taccaggaga tttaaaggac aaagttgacc catatttaag accactatat gatgccttat
    13261 ttgaaatgtt gggtgctgat aaatttaata agtatttaga aagaggtact attgaggttg
    13321 caccacttgc atttatgaga ggtagaacac ttgataattc atttataata ttggatgaag
    13381 ctcaaaatac tacttctgaa caaatgaaaa tgtttttaac tagacttgga tttggctcta
    13441 aagctgtagt tacaggagat gtaactcaaa cagatttacc acaaaataag aagagtggtc
    13501 taatacaagc tacagagatt cttaaaggtg ttccaggtat tgggtctata atgctaacag
    13561 atagagatgt tgtaagacat gagttggtac aaagaataat aagagcttat gagaagcacg
    13621 acaaaagaga agaatttaaa aaagaagaac gtaaaaaagc gaaaatgcag gaaaagactt
    13681 tcaaaagaaa gtaaaagtgt agtaatatag atatatagca ttattaatga ataaaaagta
    13741 ggtgtagtga atggatttga tactagatga tagacaagat aaattagaag taagtgagga
    13801 gttaattgaa aagataaagg atataatcat agaatgttta gattatgaag gatatgatga
    13861 taattatgaa gtgagtcttt cttttgtaga taataaagag attcatgaat taaatagaga
    13921 atatagagga gttgatagag tgactgatgt tttatcattt cctcttttaa gtgatgattt
    13981 tgaggatgtt gagttagaag aagaatccct aggtgacata gtagtatctc ttgaaagagc
    14041 cttagaacag agtatagagt ataaccatag ctttgaaaga gaagtatgtt ttttaatatg
    14101 tcatagtatg tttcatcttt taggttatga ccacgataca gatgaaaata caaaagaaat
    14161 gagagaaaaa gaagaacaca tattaaataa gttaaacata acaagggagt agtttttatg
    14221 aaaccagaaa aaactcgtca aggtataatt aaagcattta atgcagctat agaagggata
    14281 ttgtatacat ttaaatttga aagaaatatg aaaatacatt atttaggttc agttgctgtt
    14341 cttataataa gtttgttttt taacttttca aaattagaaa tgataatgtt acttatgtcc
    14401 atatgcttag ttgtagttgc tgaaatgttt aatacagcta tagaaaaagc tgttgattta
    14461 gtaactgatg aatatcatgt tcttgctaag atagcaaaag atgttgcagc tggaggcgtt
    14521 ctagtggcag cactaaattc agtagtagtt ggatatattt tgttttatga taaattgaca
    14581 gatatatctg gaatattaat ttataaaata agagaatcag aattacatat aaccttgata
    14641 tgtatattat tggttcttat agctgttgta gttgtaaaag cattgacttc tacaggtact
    14701 cctctaaaag gaggaatgcc aagtggtcat gctgccttag catttgctat agcaactgca
    14761 attacactta tgactgaaag agttgttgcc tctactcttg catatataat ggctgtatta
    14821 gttgcccaaa gtagaattga aggaaaaata catacctttt gggagactat tgctggagct
    14881 ttgttaggtg tgttgatagc tatattagta tttcagcttg gaatgttcta taattaaaat
    14941 aaatgtacat aaaatattta aaataaaaca aatctgtgct aattaaattt ttatgatata
    15001 atgtagagta ttaaataaaa ataactttta attattgtat acaggaggag ttgttgtgga
    15061 taacatagaa ttgttaaggt tggcagaaga tgccagacaa cattcttatg ctccttactc
    15121 aggttttaga gtaggtgcag ctcttttaac taagagtggt aaagtttata ctggatgtaa
    15181 tatagaatgt gcatcattag gtggaacaaa ttgtgcagaa agaacagcta tttttaaagc
    15241 aatatcagag ggtgataagg atatctataa aatagctata gcaagtgata actcagaaaa
    15301 taatgagcaa acatatccat gtggaatatg caggcaggta attatagagt ttggttctga
    15361 tataaaaata ataacgggat atactaaagg agaggtgttt gagcatagta taaaggattt
    15421 gttgccaaac tatttctctg gaaatgattt aaaataaatt taaaagtata aataaattta
    15481 agtaattaaa acaagaagat aaaaagttag gagacagtta atgttcaaat caggttttgt
    15541 cagtatagtg ggtagaccaa atgtaggaaa atctacttta atgaataatg tagttggaga
    15601 aaaaatagct ataatgagtg ataagcctca aactacaaga aatacaatac aagcagtata
    15661 tacagatgaa gaaatgcaga tagtattctt agatacacca ggtattcaca aaccaaaaaa
    15721 taaattaggt gaatttatgg taaaggcagc aacagaagct tttaaaaatg tagatttaat
    15781 attatttgta gttgatgatt ctaaaaaaat aggtcctgga gatagaaaga taatagagga
    15841 tttaagaagt gttaagacac caataatttt agttgtaaac aaaatagacc aactagacca
    15901 aaaagatgag ctatttgata taatcaaaat gtatgataga gaaggcattt ttaaagaaat
    15961 agtaccaata tcagcattaa aaggaaagaa tacggatacc ttaataaaag ttatacaaaa
    16021 ttatttagaa gaagggccaa agtattttcc agattatatg attacagacc agccagaaag
    16081 agtattaata gcagaactta taagagaaaa agttcttcac tatcttaatg atgaaatacc
    16141 tcatggtgta gctgttgaaa ttgaaaaaat gaaagcgaga aatgataagg aaatagtaga
    16201 tgtatctgct gtaatatact gtgaaagaga ttcacataag ggaataataa taggaaaaaa
    16261 tggtaggaaa ctaaaaggaa taggtaagtc agcaagacaa gatatagaat tgcttttagg
    16321 ttcacaaata aatcttcaat tatgggttaa agtcaaagaa aattggagaa atttacaaaa
    16381 ttatataaat aattttggat ataatgataa ataaaggtgg gttatatgga taggaaactg
    16441 gatgcttccc aagatttatt atatgaagta aaatcattaa ttgataataa taaagtattg
    16501 gagttaagag aattaataga ggaatatcat ataattgata tattcgatat aatggaaaat
    16561 ttagaggaag acatgaaaat tcaattattt gaagtacttc ctttagatat ggcatcttct
    16621 atattagaag aaggtagtgt agagtttttt attagtattt tatctaaact ggatgtagaa
    16681 cactcgaaaa atatacttga acttatgtct cttggagata tggcagacaa gttgagtgaa
    16741 cttgaagaag aagaaagaga acacataata aatcttttga atcaagagaa tgccgattat
    16801 gtaaaagagt tactattcta tgatgaagat tctgctggtg gaacaatgac cactggatat
    16861 atttctataa ataaagatat gacagctctt gaggctatag accatatgag agaagaagct
    16921 gaagaagctg aaactattta ttatatatat gtggttgatg atgaagaaaa actagttgga
    16981 gtattgtcat taagagaatt aataatagct agagacgcaa atatagtaga agatttgatg
    17041 agtgaaaata taatctctgt ttatgtagat gaagatagag aagaagcagt aagattagta
    17101 tctaaataca atttaatagc aattccagta gtagacagac aagaaaaatt aaaaggaata
    17161 ataacagtag atgatataat tgatgtaatg gaagaagaag caactgaaga tatgtataaa
    17221 tttgcaggaa gttcagaaca tgaaagagaa gttgctgaaa aagaaaaccc tacattgaga
    17281 gagcaaataa tatcagcact tagaggacga ttaccttggt taattattac cttagttgga
    17341 gggttgttgg cttcgttaat actttctaac ttagactata taatgaatcc tgtatatgct
    17401 ccattagtat tttttatacc tgtagtaatt ggtatgggtg gaaatattgg aactcaatca
    17461 tcatctgtta cagtaataac attatctaat aaggatttaa attttagcaa tgtggttaga
    17521 gaaggtattg taggtattat tacaggtctt ttgtgtagta taataacagg tattgtaatt
    17581 tactttgtta tgagaaagtt agacattgta ttaattgttt caatatcatt atttataaat
    17641 atggttttag gtgcaacaat aggtgcgttt atgcctgttt tattaaaaaa aatggatgca
    17701 gacccatcta cagtttcatc accaattata tctacagctc ttgatataac tggtatagca
    17761 gtttacttta taataacaac ggcattattg tcaaaaattg tttaataact ttatgtatga
    17821 tttatcctct tcttactaaa aatataagta taaatgtaag aagggggttt tttaaaaatg
    17881 aacaatatca gttgggaagt ttttaaaaaa acaggtagta tagatgcata tttatatttc
    17941 tgtgattgca aaaacataca tgaagaaatt gaagaagtaa gggagaaaaa agaagacgat
    18001 gataatcctg aacacccagg ggatagtact taaagctata aggtataagg aaagtgatat
    18061 aatacttaca ctatttacaa gaaagcttgg aaaagtatct gcaatagcaa aaggagctaa
    18121 aaaaaataag agctctttgc tttcatcatc acagttattt tcttatagca attttacctt
    18181 aaaaaaacaa ggtaatatgt acaaggttac tcaaagtgaa ataataaaaa gtttttataa
    18241 tatatcctat gatatagagg cattttctta tgctacatat attacaaaat tagttgaaaa
    18301 ttctatactt gaaaatcaga ctaataatag gctatttata ttacttgcgc agactctgta
    18361 tttgtataca caggataata cagacaatag gtttataaca gcagcattcg agttaaaatt
    18421 tttagactat ataggattta aacctatagt aaataaatgc attaattgtg aatgtaaaat
    18481 tttacaaaat tctgtgttta atatctacga aggtggtata ctatgtagta agtgtagtaa
    18541 actttttgat aataatataa aattagactt aacaactata agtcttatgg aatatgtatt
    18601 aagaaatgat attttaacat gtagtaaagc taaagtatca aaatatataa cacatgaact
    18661 tgagaatatt ctagacaagt atttgaaagt ttatgttgat aatattaatt ttaaatcatt
    18721 acacgtattg caaagtgtaa aaaataataa gggagtggat aatgatgagt agtaatataa
    18781 ctattgaaat ggttgattca gtctttgaaa gagttccagg agcaacttat gcagaagcaa
    18841 aagaagcatt agtaatgtgc aatggagatg taattgaggc agtaatatat ttagaaaata
    18901 aaaaaaatac ttgtaattgt aaagcaaaat ctgctaaaga aactatggaa gaagtatttg
    18961 gcaaagatgg agaaaatata aagtttcaat taaaagagct tcttagaaaa agtagtgtag
    19021 tgagaataat agttgaaaaa gatggaaaaa ctatgatgaa tattccttta acagttggag
    19081 ttgttggatt agcttttgta ccattagcta ctttagttgg attatcagct gctgttataa
    19141 gtaaatatcg tataaaagtt caaaatgaag aagatggaga aattgtagat ttaggtgaat
    19201 tgaatgaaga aaaacttcac gttttgaaga atatgataac taatgcagca aaagatgtga
    19261 aggatgtagt agttgacaaa aaagaagatg acaaagatgt aactgctgac cttatgaaag
    19321 aagaatctga aaagaataaa gaagacgaag acgaaaataa atagatataa agtgactttt
    19381 aaaaaatgaa gagaagataa ttataagaat tatcactaac attgacaaat tttatagaat
    19441 ttgatattct aaaagttgta gaagaactat taaaatagcc tttcgtgtag acgagaggct
    19501 ttattgttat aggagggatt tttatgaatt ttcaagagat gatactggcg ttgcaaaaat
    19561 actggtcaaa gcaaggttgt atcatgatgc agccttatga tattgaaaaa ggtgcaggaa
    19621 ctatgaatcc taatactttt ttaagatcat taggacctga gccttggcaa gtatgctatg
    19681 tagagccttc aagaagacca gcagatggaa gatatggtga aaacccaaac agactatatc
    19741 aacatcatca attccaagtt atactaaaac cttctccaga taatatacaa gaattatatt
    19801 tggaaagttt aaaagagatt ggaattgacc caagtgagca tgacataaga tttgttgaag
    19861 ataactggga agctgctaca gttggtgctt ggggacttgg atgggaagtt tggctagatg
    19921 gtatggaaat aactcaattt acgtattttc aacaagtagg taatattgaa tgtgagcttg
    19981 agacaggaga aattacatat ggattagaaa gattagctat gtacattcaa gaagttgata
    20041 gtgtttatga tttaaaatgg aatgataaaa taacttatgg tgaagtattt aataaagctg
    20101 agtatgaaaa ctcaatgtac gcatttgaat tatgtgatgc agatatgttg tttaatctgt
    20161 ttgatatata tgaaaaagaa gcattaagac ttatggaaaa tggccttgta ataccttcat
    20221 atgactatgt gttaaaatgc tctcatgcat ttaatacatt ggatgcaaga ggtgcaatag
    20281 gagttagtca aagagcttca tttataggta gagttagaaa tatggcaaag actgttgcag
    20341 aaacttttgt aaaacaaaga gaagaaatgg gattccctct tttaaaggac ggtgacaaat
    20401 aatgaaaaat tatcttttat ttgaaatcgg tgttgaagaa atgccatcaa gatttgtagg
    20461 cagtacacta gaacaattaa aaaacaatct tagtaaatta tttgatgaaa atagaatagc
    20521 ttttgataaa ataaatgcgt atgggacacc aagaagactt actttagtag tcgaaggatt
    20581 gagtgataga caaaatgatt tagaagagga aataaagggt cctgctaaga aaattgctgt
    20641 tgatgcagaa aataatttaa caaaaccagc attaggattt ataaaaagca aaggattaag
    20701 cgaatctgac atatatttta aacaagttgg aaaagaagaa tatatatttg cgactataaa
    20761 gcaagatgga aaagaaactt cagaagtact aaaagacata ttaccagaga ctataaaatc
    20821 tgtagtattc ccaaaagcta tgcgttgggg aggaaaaaat cttaaatttg caagacctat
    20881 aagatggata gttgcattgt taaatgataa agtattagag tttgatttag aaggaataat
    20941 atcttctaat gtaacaaaag gtcatagatt tttaggtgaa agtacaatag aagttgattc
    21001 tatagaagat tattttgaaa aattagaaaa aaattatata gttttagacc aaaacaagag
    21061 aaaagaaatg ataagaaaac aatgtataga agtagcaaat tctttaggtg gagaagttga
    21121 atttgatgaa gatttacttg aagaggtaac ttatttagtt gaatatccaa ctgcttttta
    21181 tggagaattt gatgtggatt atataaaact accaaaagaa gttgttataa ctcctatgaa
    21241 acaacatcaa agatattttc cagtacttaa agaaggaaaa ctgttaccta actttatagc
    21301 tgttagaaat ggtgattctt acagaataga caatgttaaa gctggaaatg aaaaagtttt
    21361 agaagcaaga cttgcagatg ctttattttt ctatagagaa gatactaaga gaaacttaga
    21421 aagctacata gaaaaattaa aaactgttgt attccaagta aaactaggaa ctatttacga
    21481 caaaacttta agacttgaaa aattaagtgc tgatatactt gatagtttag gtttagtaga
    21541 tgaaaaggca gatactataa gggctgctaa attatctaag gcagatttag ttactggaat
    21601 ggtaggagaa tttgatgaat tacaaggttt tatgggaaaa gaatacgcaa aagttggagg
    21661 agagaatgat gttgtaagtg aagctatttt tgaacattat cttccaagat ttgcaggaga
    21721 tatacttcct aagactaatg caggagttgc cttatcaata gctgataagt tggattcaat
    21781 agcaggtttc tttgctatag gaatacaacc aacaggttct caagacccat atgctttaag
    21841 aagacaagct ttaggtatac ttagtatact tatggacaga aaagttgctg tggatataaa
    21901 agtacttatt gaacatgctt tagacaatta ttctgaatta gattttgata aagaagaagt
    21961 tgttgagcaa ataatgaatt tcttcaatga gagagtaaaa aatctattta aagatttagg
    22021 aataagatat gatgttgttg atgctgtttt aagttctgat ataaatgatg tttctgatat
    22081 gcatatgaga gctttagagc ttaataattg gctagaaaaa gatgaattag tggagatgct
    22141 tactgcattt aatagagttt ctactctagc tcaaaaagca gaaagtgata aaattgatga
    22201 atctttatta aaagaagatg ctgaaattaa actttacaat gaatttaaac aagtaaagat
    22261 aaaagttaat gaattattaa aggataagaa atatggtatt gctttagatg aatttgcatc
    22321 tttaagacca gtagttgata atttatttga tacagttatg gttatggata atgatgaagc
    22381 tattaagaac aatagacttg ctttacttaa acaagtatat gatactatgc tagaaatttg
    22441 tgatttatct aaaattgttt ataaataaaa tttaaactat ttataagaaa taaggctgtc
    22501 tcaaaataga gatagtctta tttttcattg ttgagatgat gaaaatatat ttgatttcat
    22561 agaaaaagag tgtctcataa actaaaaagt tcattttgag acacccatgt tttttgtaag
    22621 taaatataat aaactatatt ttatacttat cagttatttt gtttaaaata ttatcatttg
    22681 aatttaccca ttcataaaaa ttgcctgatg atgcgtccga tattttataa agcatttcag
    22741 gaatatcact agctttaaaa tctccaagtg attttcccaa tctagtctta cctatttcaa
    22801 aactgcctcc aacaaataat tcaaatgtat ctgtagatat accatcaaca ttcttctttt
    22861 taccacataa acctatgcta ccaacttgat gaacaccaca tgaatttaaa catcctgata
    22921 tatataactt aggtgcttta tttaaaattt cttcattatt ttgtaatcta aaataatcta
    22981 tgatttcatg taacattttc tgactatttt gtatgcccat ttgacaaatt ggaacaccta
    23041 tgcaagatat actttgttct aattgagaat tacaactaat agttttactt atttctaata
    23101 ttctttttgc ttcatttcca tttaaattta aaatatataa tccctctgtc attcctagtc
    23161 taatcatagg attttcaaca ttatctaatt ctttaagtaa agtacttaaa tcctttgtta
    23221 acaaaatacc tccaactgga tgtatatata cagtgtataa accatcctgt ttttgaggta
    23281 ttagtctaga atcttgtata tctattttaa ctccaggttt agagtaatct atttcttcaa
    23341 tattgagttc aagactacca ttttcttttt ctaatttata gtattcttta aatttttcta
    23401 agaatagttc ttcaccaagt gattcgacca tatatctaac cctagctctg tttttatttt
    23461 cataatttcc ataatccata aagaatttaa tcataccttc aacacaatat aaggcatctt
    23521 tcggctttat aagttcatca agttctaaag caactttggg ttgtttacct agacctccac
    23581 ctaagaaaac tctgaaatat ggttcatcat ttttcaaagt agcaacaaat cctaagtctt
    23641 gtatactaca atgagcagta tctgtgtaac aattagaata agaaactttt aattttctag
    23701 gtaaatggta tgtagtagca tttttgataa aatatttatc agtagcaact gcatatggag
    23761 taacatcaaa gacttcacta gggtcaactc cagataatgg agataatccg acatttctag
    23821 gataattacc accaccacct ctagtgaata tatcattttt tattccttct tccatgatat
    23881 caacaattga atttatagat aaatcgtgca attgtatagc ttgtctagtt gttaagtgta
    23941 tcttatctaa gttgtgttta atagctattt catagatttt attaagttga tactgagata
    24001 aaactccagc agatactcta agtcttatca taaaagattt tttgtctctt tgagcataaa
    24061 caccatatcc accagaaaat cccttatatt gcattttatt tatttctcca tttaaaaatt
    24121 ttgaagaata ttctttaaaa ttaggtagtt catttaacaa tatcttttta aaattatcca
    24181 ttatttcacc tcattgttag tcttgtataa aaatatatct attgtataat agatatatat
    24241 attattgtct agtcaggata aaaatataac agaataattc tattaaaagt aagaataata
    24301 aggacatagt gagtattttg tttttatttt acaaattaag tataatattt ttgttttata
    24361 aaatataatt aagataaata gataattttg taattttaat atataatatt tagtagagtg
    24421 tgggagtagc acttaatatt tgcaggagga gtttttgtat atgggagaaa taataaatgc
    24481 attagataga gccctagaca taattttgct gttatatcat gaaaagagag aaatgggaat
    24541 aactgaaata agtaaggcta tgggagtata taaaagtact gtacatagaa ctttggttac
    24601 tcttgaaaac aaaggttttg ttatacaaaa tgcagagaat ggtaagtatt ggctaggaat
    24661 aaatttatat gcaataggta tggttgtggg agaaaaaatg tcattgactg aaatagtaaa
    24721 accatatacg aaaaaattaa atcaagaatt taacgaagta gttaatgtat ctatattaga
    24781 agaaagagct caagattctc caagaagtat cataatacat aaagagtatg gttcaaatca
    24841 attactttca gtaaatccat ccgttggttc aagtagtgaa tgttattgtt cagcagttgg
    24901 taaatgtctt atggcattta atgattctat agattttgaa aaatatagaa aaacaccaat
    24961 acacaagtat acagaacata caattgataa ttgggatgat atgatgcttt ttttagcaaa
    25021 aataaaagaa caaggatatg ctatagatga tgaagaacta gaacatggac taacttgtat
    25081 tggagcacct atcttagata aaaataataa agctattgca gccataagtt tgtcaggtcc
    25141 aactataaga atgagagaag gggactttga gtataagatt aaaagagtta tagaaactgc
    25201 aaaaagtata tctgaacttt ttagatgatt tttaatctcg ataataaaaa taggctatat
    25261 ttaaattgtt atttgtaaat atcagtttaa atgtagccta tttttcttta atctaaaatt
    25321 aaatattctt aaaatttaaa atgttattga aatatataag cgaatgtgtt atcttttaat
    25381 taagcaataa aaatagaaca aggttctata ctatagaaca aaattgatgc atatatagta
    25441 tttatacaaa atataaatat tatatattta aaattaagta aaaatattac ataaagggga
    25501 gatttgagaa atggaaaaag tcaagttgag tgttgaaaga tgtaagggat gctatctttg
    25561 tattgaagct tgtaaaaaag atgcaataag tctttctgac tacacaaata aaaaaggctt
    25621 tgtaactatt aaagtcgacg aagaaaaatg tattcaatgt ggcgcttgtt atacaatgtg
    25681 ccctgattta gtatttgaga tattataaat tatttttatt agaggggaga aaaggtatgc
    25741 aaagtttatt aattaaagga aatgaagctt tagcagaagg tgctatacgt ggtggctgca
    25801 gattttatgc aggttatcca ataacaccac aatcagaaat attagaaatg atggcatgga
    25861 gacaaaaaga agttggagga gtatttatcc aaggtgaatc agaaatagcc agtgtaaata
    25921 tggttatggg agcaagtgca cttggagcaa gagctatgac ttcttcatct ggtccaggat
    25981 tttcattaaa acaagaaggt attgcatatt gggtatcagc tggtttgcca gcagttgtag
    26041 catgtgtgca aagatatggt ataggtgatg gatttatagg agctggtcaa gataattatt
    26101 ggcaagcagt aaaaggcggt ggaaatggag attatcattt aatcgtgtta gcacctaata
    26161 gtgttcaaga aaatatggat atggcatatg aatcttttga attggctgaa aaatatatgc
    26221 atccagtact tatactttct gatgctacaa taggtcaaat ggtagaacct tgtatagcac
    26281 caccaatgaa agaatacgat atggataaag ctccatgggc tgcaaaagga acacctgtag
    26341 gagaaaaaaa taaagtaata actgatataa catattttga agaccaagat acatggcaaa
    26401 aagggtatat agaaaaaatg agaaaagttc aagaagatga acaaagatgg gaagagatag
    26461 aaatagaaga tgctgaagtg gtatttgtgg catacggtat ttcatcaaga atagcagaag
    26521 gtgcagttag agatgctaga gcaaaaggaa tgaaattagg tctaataaga ccaaaaacat
    26581 tgtggcctta cccaagaaaa gcatttaaga aattaagtga ttcattgaag ggacttgtaa
    26641 cagttgaaat gaacatgatg gcacagatga aggaagatgt aattatagca tgtgataata
    26701 gatttccagt atattcagta gcaacagctc aatatatggc agaagtaaat caattaatag
    26761 attttgctca acagattata gatggaaaag ctaagcaaga ggaggtattt taattatggc
    26821 taaaaaagca ccagcaataa tgatgtgtga aaatatgttc tgtggaggct gtggacactc
    26881 tatagtcgat agaattattg gagaagtatt aacagaaatg gatatatgtc aagatacaat
    26941 tatatgttca gatataggtt gtaatcatat gtttcaattt agtatgaatg tagatgttgt
    27001 cgttccacct catggtaaaa tgggagcagc aatggcagct atgaaaagag ttagaccaga
    27061 taaatatata tttacaatag ctggagatgg tggagcttat gcaatagggc ttggagaaac
    27121 tatgtcagct gctactagaa atgataatgt aatatttctt gttatgaaca ataatatgtt
    27181 tgcaatgaca ggagggcaaa tggctccaac tactatagaa ggtcaatata cagcttctac
    27241 tccagcgggt agaaattacg cagatcatgg ttcaacattt gatgttgtaa aagttatggg
    27301 aaatttagat atagcatatc tagcaagagg aacaataact aaccctaaag aaataaatac
    27361 aacaaaaaga tacatcaaaa aagctctaga aaaacaaaaa gaaaagaaag gtttttgctt
    27421 aatagaagtg cttgtaccat gtccaacaaa ctggggatta aagccagtag atgctatgaa
    27481 gagaattgaa aatgaacttg ttccaaagta tccacttgga gaatttattg attagggagg
    27541 aaataatatg ttgaatgaaa ttatatgtgc tggttttgga ggacagggag ttcttacagc
    27601 aggaaaaatt ataatgtatg ttgcatataa ggcagaaaag aaaattacat ggttcccatc
    27661 ttatggaaat gagatgaggg gaggaagtgc aaattgcaat gttgttgtaa gtgatgaaaa
    27721 aattgcaaat ccttgtgtaa aaaatccaga tatactattg ggaatgaaca gcgtatcagt
    27781 agataagtat gaaagtttaa tgaaaaaaaa tggttatatg tttgtcaatg aatcactagt
    27841 aagtaaggat agaaagtaca gagaagacat taatgttata aaagtacctg ttactgaaat
    27901 agcacaaaat ttaggcaatg aaaaagctgc taacatttgc atgttaggtg ctatggtaaa
    27961 gagtttggat ttatttacaa aagaagaatt tgaaagtggt atgtgtgaat actttgaaaa
    28021 tcaaggcaaa ggtaaattca attctaaaaa tgtagaagca tttgaagctg gatacgacta
    28081 cgaatgaata ttttaaatat ttaaaataat tattgaaata cttattttta tatgttagta
    28141 tttaattaat aaaatataga acgtcgttct ggacaatggg acaaagtctt taggtcggaa
    28201 tacaagaacg acgttctgca tagtagaact agggggtgtt aaattgaaag ttttagtaat
    28261 aaatcctggt ggtacatcca caaaaatttc tgttttccaa gatgaaaatg aaattttgaa
    28321 aaaaaatata acccatacaa gagaagattt aaaaaatttt tctaaagtat ttgatgaata
    28381 tgattataga aaacaactga tagtagatat tttaagtagt gagaatcata gtataaattc
    28441 ttttgacgca gtagttggtc gtggtggact tatgaaggct ataaaaggtg gtacctatac
    28501 tgtgagtgaa gaaatgattg aggatatgag aaatgaaata aatggggaac atgcatcaaa
    28561 tttaggtgca ttattagcta aaactatagc tgatgaaata ggagtacaat catttgtagt
    28621 agacccagtt tctgtagatg aatttgatga tgtatcgagg attacaggaa tatcagacat
    28681 agaaaaagca agttggttac atgcattaaa ccataaagct gtttcaagaa aaattgcaca
    28741 aaaactaggg ggaaaatacg aggattataa ctttattgta gctcatttag gttcaggaat
    28801 atcgattgta gcacataaac aaggcaaaat gattgatgga agtggtggta ggtctgatgg
    28861 tccattttct ccagaaagga gtggtggatt acttgcatat cctcttatag aattgtgtta
    28921 ctccggaaaa tatactcatg atgaaatggt cgcaaaagta agttcgattg ggggaatgta
    28981 cgattattta ggaacaaagg acatgataga aattgaaaac agaatcaagg atggtgatga
    29041 aaaggcaaaa cttataatgg atgcatttat ttatcaagta gcaaaagaaa tttctatgta
    29101 tggagcttca ctttcaggga atgtagatag agttatcttg acaggaggga tatctcactc
    29161 agaaatggta gtcaaaggag tagcagaaaa agtttcatat ctagctccaa tagagatagt
    29221 accaggagag atggaaatgg aggcacttgc tctaggggca cttagagtac ttaatggaga
    29281 agaaatagca aaaacttatt aatatttagg agaaagagta tgttaaaaga ttttaataat
    29341 ttattagaag aggctaaaaa gcttgaacct gttattgtaa gtgttgctgt tgcagaagac
    29401 aaagaagtat taaaggctgt gaaatcagca acagatttaa acattataaa gccaattctt
    29461 gtaggagaca aggataaaat agaaagtata tcaagagaaa ttggattaaa aggtcatgaa
    29521 ataataggtt gtcaaaatcc cgatgaatca gttaaaaaag ctgtagagat agttaaaaat
    29581 aaggaagcta aagttcttat gaagggatta gtaaatacaa gtgtctatat gagagctata
    29641 ttaaataaag aaacgggtct tagaacagga agattattaa gtttgcttgc agtatatgaa
    29701 ttacctcaat atcataaact tctttattgt actgatagtg gaataaatgt atcgccaaac
    29761 ttaaatcaaa aaaaagatat aatgacaaat gcacttctta ctatgaaaag tataggcatg
    29821 gaaaatccta aaactgcgat acttactgca aatgaaatgg tagaccctaa gatacaatct
    29881 acagtggatg ccaacctact tgtagagatg gaaaaatcgg gagaaatacc aaagtgtata
    29941 gtggaaggtc caatagcatt tgatgtagca tttgatgcac atgcagcaga acataaaggt
    30001 attaaaagta atattgctgg tgatgttgat ttattagtat ttccaaatat agaagcaggg
    30061 aatatattag gaaagtcatg gcttagattt aataaagcta agtgggcagg tatagtttta
    30121 ggagcaacaa atccgataat tttaggttca cgttctgaca cagctgaaat aaaaataaat
    30181 tcaatagcat tagcgtgcct agcatcacaa taagaaatta ttattaagga ggaaaaagta
    30241 ttatgaaata tgcaaaaaga atggatatgg taaaagcttc agctataaga gattctcaaa
    30301 agaaaatagc tcagaaggta gcaagtggtg gaactgttat atcttttgct gcagggcttc
    30361 ctgacccaaa tttatttcca atagaagaga taagtgaaat aactcaaaaa gtacttaatg
    30421 aaaggggaaa atttgcccta gcatatggac ctactaaagg tgaagatgag ttgttagatc
    30481 tattagtaaa aagaatgaaa gaagaagaaa atattgatac aaaagcagaa aatattataa
    30541 taactactgg gtcacaacaa gggatagcat taagtgctat gattttatta gaaaagggag
    30601 atgtagtagt tacagaaaat ccaagttact taggtgcaat aaatgcattt agaccttatg
    30661 aatgtgattt tttaggagta gatacagatg aagaaggaat ggtaacagat gatttagaca
    30721 aattattatc tgaaaattct aacataaagg tgatatatgt aataccaaca tttcaaaacc
    30781 ctacaggaaa aacatggtct ttacaaagaa gaaaagattt tatggaagtc gtgaacaaat
    30841 atgatgtcat agtaatagag gataatcctt atggagaaat aagatttaca ccagaacctc
    30901 tacctacact taaatctctt gatactaaag ataaagtatt gtaccttggg tcattttcaa
    30961 aggtattatg tccaggattt agagttgctt ggatgtgtgg taatgtagac atcatagata
    31021 aggcagaact tttaaaacaa ggtatagatt tacagtcaaa tcaattttcg caagtacaag
    31081 taatagaatt tttaaagaaa tacaacttaa atgaacatat agaaaaaata agagcagagt
    31141 ataagaaaag atgtctgcta atgttagatg ctatgaagaa acatttccca gaggaagcta
    31201 aatatacaga accagatgga ggaatgttta tatgggttga acttcctgaa agtataaatg
    31261 tagatgattt attagataaa gccattgatg ctggagttgc ttatgtttct ggagagtcct
    31321 tctttgcaaa taatggtcca aaaaatacta tgagacttaa ctatacaaca atgtcagaag
    31381 aacaaatcgt taaaggtgtt gagatacttg cagaagttat aaaaaaagaa ctcaattatg
    31441 cttgtgaata aatataaaaa gtgagggttt aaatatggga gaaaaagaaa acggtataaa
    31501 gtttggaaag ttaatgtttc aacttgttat tatagcaata tcttgggaat tagtttatat
    31561 tataccattt atccaatata cattatatga tccaatccta aaagcattag aatgtacaaa
    31621 cactcaacta ggttttctac ttacaattta tgggttaggt aatatatttg gagctcctat
    31681 aggaggctgg ttagcagata gatttgatta tagaaagata attttaggtt ctgtatttct
    31741 taatggagta gtatcatttt tatttgcatt taatatgaac tatccatttg cagttgctac
    31801 ttgggtaggt tgtgcaatca catcattagt tatgaactat ccttctatgg taaaaatact
    31861 tagagttata ggaaaagata atcaaggtaa ggtttatgga tttaatgaag ctatggttgg
    31921 agtttctgga gtagtaatgg gagcaatatt cttatatatt tatacatgct ttgcaactcc
    31981 tacattgggt atgagatggg taatgatatc tttaggaata ttatcaatag caatgtgtcc
    32041 agtattatgg tttgtaatta aagatgtaga tattaaagaa gaaagagaag aagcaggtga
    32101 aaaaatgtct gctggtgatt ttataacagt actaaaatca ccaaatactt ggctagtagg
    32161 aataagtata ttctgtgtat attcattcac agttacaatg tcttatttta caccatatat
    32221 aacatctgta cttggaggtt ctgtagcatt atctggtgca ttagcaataa taagacagca
    32281 tggtctaaaa ttatttggag caccatttgg aggatattgt gcagacaaat taaaatctcc
    32341 tacaaaagta cttttaccaa tatatgtatt tggtatagca gtaatagttt tattcctagt
    32401 attacctgca tcaactccaa tgactatatt catagcttta acatttgtag taggtatatt
    32461 aggttacatg ggaaaaggta tatattatgc agtacaagat gaagtaaaag ttccagttaa
    32521 atattcagca actactatag gtatagctgc agctttagga ttttcaccag atgtattcca
    32581 atttgcatta ataggacact ggatagatac ttatggcaat aaaggttaca catatacatt
    32641 tatattccaa atagcaattc tagttattgg aatactatct tgtttatata tattaaaggt
    32701 taaaaagaga agagcaagta aattagaagc ttaaatattc aatttggtaa ttaagtatat
    32761 ataaatacaa tacttatttt gatgaatgtg attgtataaa taacataaaa aatatcaata
    32821 gattaagttt tataataatg tttagagaat aaagtataaa aatcaaaatg ttatcaagtt
    32881 ttctaggttt taacataaag ttacccctga aggtgtaaaa ttgagtggca attattatct
    32941 attttcttac c
//
LOCUS       DAELNY010000022        32755 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.022, whole genome shotgun sequence.
ACCESSION   DAELNY010000022 DAELNY010000000
VERSION     DAELNY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.022"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(120..965)
                     /locus_tag="KPE55_000936"
     CDS             complement(120..965)
                     /locus_tag="KPE55_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0492032.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            1182..2348
                     /locus_tag="KPE55_000937"
     CDS             1182..2348
                     /locus_tag="KPE55_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG0492033.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            2370..4016
                     /locus_tag="KPE55_000938"
     CDS             2370..4016
                     /locus_tag="KPE55_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG0492034.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            4113..5486
                     /locus_tag="KPE55_000939"
     CDS             4113..5486
                     /locus_tag="KPE55_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0492035.1"
                     /translation="MNTEVNVKATTIKYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            5931..6884
                     /locus_tag="KPE55_000940"
     CDS             5931..6884
                     /locus_tag="KPE55_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG0492036.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6922..7884
                     /locus_tag="KPE55_000941"
     CDS             6922..7884
                     /locus_tag="KPE55_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG0492037.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7909..8895
                     /locus_tag="KPE55_000942"
     CDS             7909..8895
                     /locus_tag="KPE55_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG0492038.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8908..9954
                     /locus_tag="KPE55_000943"
     CDS             8908..9954
                     /locus_tag="KPE55_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG0492039.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            10121..11851
                     /gene="lpdA"
                     /locus_tag="KPE55_000944"
     CDS             10121..11851
                     /gene="lpdA"
                     /locus_tag="KPE55_000944"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0492040.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSMPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVAAVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            12269..15217
                     /locus_tag="KPE55_000945"
     CDS             12269..15217
                     /locus_tag="KPE55_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0492041.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEVISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            15195..15659
                     /locus_tag="KPE55_000946"
     CDS             15195..15659
                     /locus_tag="KPE55_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0492042.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15772..16074
                     /locus_tag="KPE55_000947"
     CDS             15772..16074
                     /locus_tag="KPE55_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0492043.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            16136..17500
                     /locus_tag="KPE55_000948"
     CDS             16136..17500
                     /locus_tag="KPE55_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0492044.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            17535..18008
                     /locus_tag="KPE55_000949"
     CDS             17535..18008
                     /locus_tag="KPE55_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492045.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYSIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYELKDIEKIRIREFYNKKI
                     YLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            18180..18830
                     /locus_tag="KPE55_000950"
     CDS             18180..18830
                     /locus_tag="KPE55_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0492046.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            19004..19936
                     /locus_tag="KPE55_000951"
     CDS             19004..19936
                     /locus_tag="KPE55_000951"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0492047.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            20210..20923
                     /gene="ispD"
                     /locus_tag="KPE55_000952"
     CDS             20210..20923
                     /gene="ispD"
                     /locus_tag="KPE55_000952"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG0492048.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVKETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20920..21405
                     /locus_tag="KPE55_000953"
     CDS             20920..21405
                     /locus_tag="KPE55_000953"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG0492049.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21500..21708
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21772..23487
                     /locus_tag="KPE55_000954"
     CDS             21772..23487
                     /locus_tag="KPE55_000954"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0492050.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23609..25054
                     /locus_tag="KPE55_000955"
     CDS             23609..25054
                     /locus_tag="KPE55_000955"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0492051.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            25493..26974
                     /locus_tag="KPE55_000956"
     CDS             25493..26974
                     /locus_tag="KPE55_000956"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG0492052.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            27195..27350
                     /locus_tag="KPE55_000957"
     CDS             27195..27350
                     /locus_tag="KPE55_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492053.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            27498..28895
                     /locus_tag="KPE55_000958"
     CDS             27498..28895
                     /locus_tag="KPE55_000958"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG0492054.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            28880..29293
                     /locus_tag="KPE55_000959"
     CDS             28880..29293
                     /locus_tag="KPE55_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG0492055.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            29399..30157
                     /locus_tag="KPE55_000960"
     CDS             29399..30157
                     /locus_tag="KPE55_000960"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG0492056.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            30170..30907
                     /gene="rlmB"
                     /locus_tag="KPE55_000961"
     CDS             30170..30907
                     /gene="rlmB"
                     /locus_tag="KPE55_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG0492057.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            30910..31437
                     /locus_tag="KPE55_000962"
     CDS             30910..31437
                     /locus_tag="KPE55_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG0492058.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            31502..32149
                     /gene="sigH"
                     /locus_tag="KPE55_000963"
     CDS             31502..32149
                     /gene="sigH"
                     /locus_tag="KPE55_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG0492059.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            32223..>32755
                     /locus_tag="KPE55_000964"
     CDS             32223..>32755
                     /locus_tag="KPE55_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBG0492060.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT        13323 a         3440 c         6177 g         9815 t
ORIGIN      
        1 gaaagtttat aaaaaataaa ttataataaa aaagagttta tctcaaaatt gattattcta
       61 atcaaatttt aagataaact ttttttatta taaagataaa taacatataa cctcatattt
      121 taggtagatt tttttcttat atgtttagga gtgcatttaa atttttcctt aaaaattctg
      181 taaaataatt ttgtattagt aaatccatga gcttctaata tactgcataa ggtatcattt
      241 gtgttaatta aatctttata aatatgagaa atacgcacat catttaaata ctctaagtaa
      301 gtttgtccca tatactgttt aaattttcta caaaaatatt ctggttgtaa gtgtgctacc
      361 ttagcgattt tgttaataga aataggcatt ttgtaattaa tctttgtata ttctaaaact
      421 ggttcaaatt ttgacaaatc aagtactgtt tttttcatat ttttattacc tacagatatt
      481 ttaaagtttg tatataattc aaataaaagt tcaaataata agcttgtaaa gagcagatta
      541 gctgcaggag gagaactaag tttaatttct ctcatgtttt ctaatataag tttaacttta
      601 cttgaatttt ttttatagtt attgtcattg gcattaacct taaagtcaaa atagcaattt
      661 ttaaaatcag ggatatattt gtttagaaaa gacaatggta cttgcaataa tatagaggta
      721 ttttgataaa tacatctagt tgagtgaata attccagaat taatgaacat acactcatta
      781 gcttttaaat tataagtgta accctccatg ttaatttgta aatcaccaga agttatataa
      841 attatttcta atgagttatg ccaatgattt gctacataac taccactatc atcaaatagt
      901 ttgaaattaa catctaaatt atcaataggt tcaattattt catgtgaaaa attatttctg
      961 ttcataataa tatcctcctt ataattttaa attataaata gtaaatttat aatgttcaat
     1021 attaaaaaga taatatagac taataaatta gataataaac atacttaaga aaacagataa
     1081 aatcaaatat aataactgta tattaaaata acttaaaaaa attattttat tataagtgca
     1141 aaatatacct atttaaataa gataagaggg gaggatttta tgtgtatata aaaggtgtaa
     1201 atttaggagg ttggttagtt ttagaaaaat ggatgacctc aagtttattt gaaggcacag
     1261 aagctgaaga tgaatactat ttaccaagac agctttcaag agaggcatat gaatctagaa
     1321 ttaagactca tcgtagtgaa tacatcacag agagagattt tgcaacgatt aaatctatgg
     1381 gatttaattc agttcgtata ccagtaccat actttatttt tggagattgt gaaccattca
     1441 taggatgtgt taaagaactt gataaagcat ttgcttgggc agataagtat gggcttagta
     1501 tattgataga cttacataca gttccaggaa gccaaaatgg atttgataat ggaggaatat
     1561 caggtatatg tagctggtca caaaatccag aatatgtagc gtttacatta aatgtattag
     1621 aaagacttgc aaaacgttat gggatgaggc atgaattgta tggaatccaa attttaaatg
     1681 agcctataac agaaagaatg tggaacataa tgaatgttcc aaatcggttt aaggctgtag
     1741 ataaggagat ggcaaggggg agtaaaccta attcattaga gtttttacgt gatttttata
     1801 taaaagcata taaggtaatg cgtccttata tgagagagga aaacgttata gtatttcatg
     1861 atgcatttga attaaaagca tggaaagact ttatgagaga ggaagaattt aaaaatgttg
     1921 ttctagatac acaccaatat ttgatgttag ctgaagcaga tggatgtgaa caaagtattg
     1981 atagctatct caaatatatt agagaaaatt atgctaaaga tattttacaa atgcaaaaat
     2041 attttcctgt tatatgtggg gagtggagct tatttaattc atatgcttgt ggtatagata
     2101 caaatggagg tcaatcacct ctaaatggga ttgaatcaaa tatagataaa ttatcaaaag
     2161 acgataaaag agaattatat aggaagatag caaaagcaca gcttgatgca tggagaaatg
     2221 gaagtggtca ctactattgg aattataagt tattactaga tacagttaat gaagaaggtt
     2281 ggataggatg ggatagttgg gacctaggca aatgtgtagc tcaagaatgg tatcctatag
     2341 aatattaaac taaaattgga ggaaaagata tgattaaaaa ccctatactt ccaggtttta
     2401 atccagatcc ttgtatttgt agaaagggag atgattatta tcttgtagtt tcatcttttg
     2461 aatggtttcc aggtatacct gtttatcact ctaaagactt aaaaaattgg gagttatata
     2521 ctcatatttt gacagatgaa acaaaaatag atttgaagaa gcttccttca tctaaaggaa
     2581 tttgggcacc ttgtttgact tattgtgaag aagaagattt attttacata gtttatggaa
     2641 ttatgaactc tatgaatgca agatattttg atgttgataa ttatttaatt acatcaaaag
     2701 atataaaggg agagtggagt gaaccagtat atctacactc atcaggtttt gatgcgtcta
     2761 tttttcatga tgatgatggt aaaaagtgga ttgcatcatt agactgggag actagagaag
     2821 gatatgaaaa acctggtgtt atatgtttag tggaatactg tacaaaaaag aaagaaatag
     2881 taggatatcc aaagagaata tggtcagggg gaactgatag aggttgtatt gaagcaccac
     2941 atataacaaa gcgtggagat tactattata taatgtgtgc agaaggtgga actggatatg
     3001 gtcatagtgt aactatggga agggctaaaa atatatgggg accttatgaa aaagattcaa
     3061 tgaatccaat tgtaacttca attccaggcg atttttatga aagacatgac cctgaccatc
     3121 taaagcctaa atactataat ccagaatcaa aacttcaaaa atctggtcat ggaagctata
     3181 tagaaacaac atcaggtgaa gtatatttag ttcatctaac atctagacca tttgtaccag
     3241 agttaaggtg tactttaggt agagaaacag ctattcaaaa aatgaaatgg acaaaggata
     3301 attggcttcg aatggaagat gaaagtaatt tggcaaaaga atacgtatca gaaagtaaac
     3361 tagaagaaca tttagtttca tctataccaa gttttgatga ctttgattca aatgaattag
     3421 gtttacagta ctatgctcct aggatttcac cattatcatt tgcagatgtt aagtctagac
     3481 caggatatgt aaggattaga ggacaagaat caagaacatc attaaataaa gtgagcatat
     3541 tggcaagaaa attaacttca gtatatgcaa gaataaccac taagatggag ttttacccag
     3601 aggtacatca gcatagtgcg ggtttgataa tgtattatga taatatgaat tatataaatt
     3661 taagaaagta ttatagtgaa acattgggtc aaagtgcact atcaataata catcttgaaa
     3721 atggagagaa aacggaattt ttaaatacta ggataccaat aaaagatata ccaatatatc
     3781 ttcgtctgta tatacaaggt cgtaagtcat attttgaatg gagttatgat gaaaaaaact
     3841 accagagaat aggaaaggta tttgatacaa ctaagttttc agatgagtac tgtaaatatg
     3901 gagaatttac aggtacgttt ataggattaa cttgtgcaga tcgtgtaaag cataaacact
     3961 atgctgattt tgattttttt gaatatatag tagatgaatc aaaagatgtc gattaataaa
     4021 atcattacta aaattttcag agcaaaggta aatcttatgc tctgaaataa gaaacattag
     4081 aaggcaaatc ttacaaaaag gagtatggta ttatgaatac agaagttaat gttaaagcaa
     4141 ctacgattaa atatgaaaag atttcatttt ttgaaagaat ttcttatggt tgtggggatt
     4201 tggggtgcaa tatcatatac tcagctatgt cagcattttt gctgttttat tatactaatt
     4261 atgcagatgt tagtgcagca gctgtaggta gtattatgct tgtatctagg atactggatg
     4321 ggtttagtga cttaacaatg ggaattatag tggatagaac taaatccaaa tacggtaagg
     4381 ctaggccttg gattttgaga atggcgattc cttttgctat agcagcagta ttattatttt
     4441 ctgttccatc aaatcttggt atcacttcta aattaattta tatttttatt acatataact
     4501 tagtttctac tgttatttat acagcaatca atgtacccta tgcaactcta aattcactta
     4561 taactcaaaa ccagtatgaa agaggagtat taagtatatt tagaatgata ttagccactt
     4621 gtggtacctt actaataaat ggacttacac tacctctagt tgaatacttt ggaaataatt
     4681 tatcagcatg gactaaaaca ttctttgtat ttggaatagc atctataatg gtatttttta
     4741 taacatttac tggaaccaag gaaagagtaa aagcagttaa gcaagatagg aatgaagtaa
     4801 taccatttaa aataggtatt aaatctttat ttagaaataa gtactggata caaataacat
     4861 tatgtttagt ttgtatattt attgtatttg cgataaatgg tggttcatcc gtatattatg
     4921 caaagtttat tttgggagat gaaaaattat ttggtcctat aaatatggtt tcaaatatat
     4981 cccaaattat agctatgttt atggttgctc cttttataaa aaaatttgga aaaagaaatg
     5041 tattaatagt aggttcaata atattaatat cgtcaaatgt tatgtttata atagttggac
     5101 aaaatcatat aggagtaata tttgcaagtg ctataaaggg aataggaagt gctggaatag
     5161 catcaacaat gtttgctatt gtttcagata ctattgaata tggagaatgg aagactggat
     5221 atagaactga ggggcttata aatagtgcat ctagttttgg gtttaaagtg ggaaatggat
     5281 taggttcagc aatattaggt gcagtattat caattggtgg ttatgtagga acatcagcta
     5341 ctcaaagtga tttagctata gtatcaataa aagtatgttt tatataccta cctatattta
     5401 taacaatatt acaaattatc ataatgtctt tttataagct tgataaagaa tatagtacta
     5461 tacttaatga attaaatact aaataatgaa aaagtttctt ataaagtctt aagtttaatt
     5521 aaggtctatt gtaaatgttc cctataaaaa ctattatata tttatgatta ttgaagaatt
     5581 tttagagaag cgaataataa aaatataaga agactttaaa tgatgaaatg gttggttttt
     5641 tagaaatgat ttaataagtt attgttattt aaagcattat aagtggacaa ttaaatttta
     5701 atttatggag atgatattta acaattagaa gtaatatata atatgcacta agaagcaatt
     5761 tttcaactat ttatgaaggt ggtggtacaa gaaaagtatc accttttttt tgttatacaa
     5821 aaaaacagtt gactttttat atgaacccct tctataatgt tactgaaatt aagtaaaatg
     5881 attgaacaaa tgtaaagata attgaaaagc ttgattttgg aggaaagcaa atgaaaaaaa
     5941 ttggagactt aagattaatg atgaagtgtt gttcacttta ttacgaagat aatttgaatc
     6001 aacaggaaat agcaaatcaa ttgggaatat caagaccaac aatatcaaga atattaaaag
     6061 aagcttttga acaaggaata gtaaaaatac agatagttga tgttttgaaa aatgattacc
     6121 aaaaaataga gagaagtttg gagagaaaat ataaattaaa agaagtaata gtagtagatg
     6181 ataaacagga tgcactaaca caaaaacaag aactagcaag agctgtatct gaatatttaa
     6241 ctagagtagt aaaggaaaat gatataatag gtgtttcaat aggaacaaca ctaaaagaaa
     6301 taccaagata tgtggaaaaa agtaactgta aaaatgttac atttatacct ctattaggag
     6361 ggattggaga caatgaaata gatattcatg caaatcaaat agcagtaagt ttagcgaggg
     6421 cttttggagg tgattttaaa ttattacatg ctccagctgt tatgtctgac ttgtctacaa
     6481 aagagaaact ttgcaaagac gaaaaaatta aagaagtact agatttaata gataaaacaa
     6541 ctatagcaat tgtgggtata ggaaatccta tgagtttaaa ctcaacaata atggcttctg
     6601 gatacatgaa tgaagcagat atagaagatt taaaaaaata caactcaata ggtgcaattt
     6661 gtttacaagc ctttgataaa gaaggaaaaa cttctatact tgaatttaat caaagagtat
     6721 taggagtgaa actggaagat ttaaaaaaga taaaaagaac aataggtgtc gcttcaggag
     6781 atgaaaaaat agaggcaata aaggcatcat taaaagcgaa atttataaat agtttagcaa
     6841 ttaatcacag tttagcactt aaattattag aagattgtga ctaatagaaa gatagataaa
     6901 agttaacagg agggaatgta tatgtcaata tcaaaagaaa cattgcttga aatgtataag
     6961 agaatgaatc aggctagaaa atttgaagag aaagttagtt ggttttttgc aagaggtatg
     7021 gttcatggaa caactcactt atctgtaggt caagaagcgt caagtgtggc ggctgtaatg
     7081 gctttagaaa aaggagattt agtatcatta actcatagag gacatagtca atttatagga
     7141 atgggaatag acctaaataa aatgatggct gaacttatgg gtaaagaaac tggattttgt
     7201 aagggaaaag gaggctcaat gcatatagca gatattgagt caggtaactt aggagcaaat
     7261 ggagttgtag gtggaggtct tacaatagct ccaggagctg cacttacaca acagtataaa
     7321 aaaacaggca aaatagtttt atgtagtttt ggtgatggag catcaaatga aggtactttt
     7381 catgaaggaa taaatttatc ttcaatatgg aaattgccaa taatatttta ctgtgagaat
     7441 aatttatacg gtatgtcaac aagcataaaa cgtcatatga atatagaaag tattgccact
     7501 agagcagctt cttatggaat agaaggaata tcaatagatg gatataaccc aatagaagta
     7561 tacgaaacag tacaaaaagc tgctgagaag tgtagaagag gagaaggacc tgtattaatt
     7621 gaaagtagaa cttatagatg gttaggtcat tcaaaatcag atgcaaatgt atatagaaca
     7681 aaagaagaaa tagaatcatg gaaagcaaaa gaccctattg aatttttaaa aaattatctt
     7741 atagaaaata atctatctaa tgaagatgaa ttagataaga ttcaagaatt tgcaaagcaa
     7801 tcaatagaag atgcagtaga gtttgctcaa aatagcccaa atccaaaaat agagtcttta
     7861 ttagaggatg tatatgcaga ctaagaatgg taaaggagga aaataaaaat gagtacaagg
     7921 gaattaacat atgcacaagc aataaaggaa gcaatgtcag aagaaatgag aagagatgaa
     7981 aatgtaatat ttatgggaga agatatagga atatatggag gagcatttgg tgtatcagtt
     8041 ggtatgatag atgaatttgg accagaaaga gtaagggata ctcctatatc agaagcagct
     8101 atagcaggag cggcagcagg tgcagcagca actggcttaa gacctattat ggaagtaatg
     8161 tttatggact ttgtaacaat ttctatggat gcaattgtaa atcaagcggc aaaaatgaga
     8221 tatatgtttg gaggaaaagc acaagttcca atggttgtta gatgtccagg aggttctgga
     8281 actggttcag cagagcaaca ttcacaaagt ttagaagctt ggttttgtca tgtaccagga
     8341 gtaaaagtag ttgcaccttc tacaccagca gatgctaagg gattgttgaa agcagctata
     8401 agagataata atccagtcat atttgttgag aataaattgt tatacagaaa aaaaggtttt
     8461 gtgccagaag atgattatgt aattgaaatt ggaaaggcag atattaaaag agagggtact
     8521 gatgttacag taattactta tggaagaatg ttacaaagtg ttgaagaagc agcagagact
     8581 ctatcaaaag aaaatataaa tgttgaaatt atagatttaa gaactttata tccattagat
     8641 aaagagacta tagtaaagag tgtatgtaaa actgggagag ttttaatttg ccatgaagca
     8701 gctaagactg gtgggctagg tggagaaata tcagccctga taacagaaag tgaatcattt
     8761 gattatttag atgctccagt aaaaagaatt tgtggaaaag atgtgccaat accatataat
     8821 ccagagttag aaaaagcagt tgtcccaaga gttgatgaaa tagaagaagc aataaaatct
     8881 ttaattgtta gataaggagg gatagatatg gtggcaaata aaataaaagc cacaccagca
     8941 gctagaagtc aggcaagaaa agacaatata aaattagata ggctgattgg aagtggagaa
     9001 aatggaagga ttcatttagt agatgtgtta aattatttaa aagataataa agcaaatact
     9061 actcctttag ctagaagaat agcagaagat ttaaatatag atttagaaac tattgtaggt
     9121 actggatata atggaaagat aagaaagtgt gatgtggaaa aattaactgc aaaagaaact
     9181 attgtatcaa ctaatacatc taaatctagt gagaaaaaag agctgaaaat tgaaaatgaa
     9241 aattcaagca tgtttaacac agtagaagga atatttgaaa agccaaatcc aatgagagcc
     9301 actgtagcaa aacgaatgtc agaaagttat tttagtgctc ctgtatttac attcaatata
     9361 gaagtagatg ctacagaatt gaaggtactt agagcaaagt taatcgacac agtaaaagaa
     9421 tcaactggag taaaattaac tatgacagat ttaatagtaa tggcagtatc taaaatactt
     9481 ccaaatcatc aagccttaaa ttcagcatgg acagatgagg gtatatttag atataaagat
     9541 gtaaatatag ctattgcagt tggtttagat gaaggcttat atgttccggt agtaaaaaat
     9601 gcaaacaaaa agtctttaaa agaaatagca aaagaaagca aagaacttgc ggaaaaagta
     9661 aagacaggga aacttatgcc agcagaccaa gaaggtaata catttacaat aagtaatgtt
     9721 ggaatgtatg gtataactac atttacacct ataataaata tgccttcaag tgcaatctta
     9781 ggggtaggtg ctacacaaga taagtttgtt ccagtaaatg gagaggctaa aataaaacct
     9841 ataatgaact tatcattaac atcagaccat agagtaatag atggaacagt agctgcaaaa
     9901 ttcttaaaag atttaaaaga attgttagaa aatcctttat caatgcttgt ataggttttg
     9961 atattttgtt actgatataa atagtttgaa tagattatat tattataatt gtttcagtaa
    10021 atttaaagct tttatatatt aagaattatt tttaacagta actatatgtg aatatgattt
    10081 gcttaattaa aatataagaa aataaatagg aggagagcct atgtcagtag aagtaataat
    10141 gccaaaagca ggagtagcca tggaagaagg tactatagta tcttggctaa agcaagaagg
    10201 cgaagaagta aagattggag aacctatact agaaataaca acagataaag ttaatatgga
    10261 gatagagtca gaaggagaag gtacacttgc tgtaatcata cataaagagg aaggtgaagt
    10321 attaccagta tttacagtaa taggtgtaat agcagaaaaa ggggaaaatc aagaggaagt
    10381 aaaagctaaa tatttatctg gaaatgtttc aaaagaagat acagttaaag agaatcaaaa
    10441 tatagaagtt aaagaagaaa aaataaacaa aaaagagtgt aatcatgatt atgatgtagt
    10501 ggtaatagga ggaggacctg ggggatattt atcagcatta aaagcagctc ttttaggtgg
    10561 aagagttgct ttagttgaag aaaatatact aggaggtact tgcttaaata gaggttgtat
    10621 ccctacaaag acttatataa aaacagcaga aatattagaa gaaattgacc aattatcaaa
    10681 gagaggtgta aaagtaactg tagataaaga acaggacatt aagaaggcca taaaatataa
    10741 aaatagagta gttaaaaaac ttacagctgg tgttggcgga ttattaaaaa gtagagatgt
    10801 agatgtgttt aatcttaaag ctagtgttaa agaagagcat aaagtaattt tatcagatgg
    10861 aaaagtttta gatacagaaa atataataat tgctacaggt tccaaagtaa ggatattacc
    10921 tataaaagga atagaatcaa atttaataat aactagtaca gaagcattag atttggaaac
    10981 agtgccagaa gaattagtaa taattggtgg tggagtgatt ggttgtgaat ttgcagagat
    11041 attcaattca agaggttcta aagtaactat agtagaaatg gaagatagag taatcccaag
    11101 aatggataaa gagttaagcg aatctttaaa atattctctt agtaaaaaag gtataaatgt
    11161 attaactaaa aagaaagttt ctgaatttaa agaagaagga aataacatat tagtgtgcat
    11221 tgaaggtgaa gagccaataa aagcagattt atgtctatat gctataggaa gagaagctaa
    11281 tctttctggc atagaggact tagatattaa aatagataaa ggttcaatag ttgtaaatag
    11341 taaaatggaa acaagtatgc caagcatata tgcagtaggt gatgttactg gaggtgtaat
    11401 gttagctcat gctgctttta aaatgggaga ggtagcagct tcaaatgctc taggtgtgaa
    11461 taaagaagtt gacttaggtg cattacctag ttgtgtttat acaattccag aagtagcagc
    11521 agttggtatt acagaagagg atgctagaaa aaaatataat gtaaaagttg gtaagtttaa
    11581 ttttgctgga aatggaagag ctttagcttc tggtcaagaa caaggatatg tgaaggtagt
    11641 agcagatgct aaatatggag aaattttagg tatacatatg tttggatgtg gtgtagcaga
    11701 acttataaat catgcagcat catttaaagc gttagagata cctactgatg aagcaagtga
    11761 gttgatattt ggtcatccat gtacttcaga agctttaatg gaagctttgg cagatgtaaa
    11821 tggagaatgt ttacacttac ctaaaaaata aatctaaaca taaattatta atttaagtct
    11881 attattaata ttaatttata tcataaaatt ttaaaacact tacttagcca taccccttat
    11941 tatgcaaata atttatttgc ataataaggg gtatttttta tggaaaaata tagaatatag
    12001 aatccttgta gtttttgttg tacaaaaaag atagtaatat aaaataacat tatcatttta
    12061 gaatgatttc aattgtaatt aggaaaagtg atttttttgt ttttaaaaaa agtattattt
    12121 gtaaaatata aaaaaaaatt tcttaatatt tgaataaaag aatagcttaa tttaaatgtt
    12181 attactttat aaaaaaataa taagtacaaa aaaaacaata tatggcatat aagttgctat
    12241 ataaataata aggagggaac aattataaat gaggcaaaga ataaaaaact taattgaaaa
    12301 tgaagataag aaaaatcctt taacagatga agtgatttca agtaggttaa atattcgaag
    12361 agaagatgtt acctttttga gaaatgagtt aaaaatagaa gattcaagac aaagaagaaa
    12421 agttgtttta attaaagcta taaaagatat tttaaaagaa gaaaaaacta taaataaaaa
    12481 tgatataaca agaagattaa acagcattgg gtttacagta tcaagattta cagtcataca
    12541 atacttaaaa gagattcaag attctggtga cctaaaactt gataaagaga gtgatgataa
    12601 aaaagaagaa ataatcaata atagagaaaa taaagatata gcatttgata aactcatagg
    12661 tagtagtggt agcttaagta caattataaa actagccaaa gcagcaattc tttatccacc
    12721 tcatggtctt catactatta ttatggggcc aactggtgtt ggaaagagtg agttagcaga
    12781 gtgtatgtat aaatttgcag tagaatcatc cagattccct aagaatgcac catttatagt
    12841 ttttaatgca gcagattatt cagagaatcc acagcttttg ctatcacagt tatttggaca
    12901 cgttaaaggt gcttttacag gagctgatga gaacaaagaa ggattagtat caaaggcaga
    12961 tggaggaata ttgtttatag atgaaataca tagattacct catgaaggtc aagaaatact
    13021 atttcaactg attgataaag gtatggtcag aaagttagga gaaacaagac taacacataa
    13081 aatagatgta atgattattt cagcaacttc tgagccaata gattctcatt tacttaatac
    13141 atttaaaaga cgtatacctg taacaattga aataccagaa ttagttgcaa gaccattaaa
    13201 tgagaggttt gacattataa ataatttctt cttaatagaa gctcaaagaa tgggagcaaa
    13261 catacatata aagtcagatg tattaaaagc tcttatgctt tacgattgta taggtaatgt
    13321 aggtcaactt agaagtgata ttcaagtagc ttgtgctagg ggactattaa accaacttac
    13381 aaacaaacta aaagaagtaa atataagcat atcagatgta cctggatatg taaaacaagg
    13441 tcttataaaa atcagaaatt gtagaggcaa aatagaaaat tatgtagatg gggatttaat
    13501 tatagattca aatcttcaag gtgagataga aaagaaaaat gaagatatat atacttttcc
    13561 agatgaaata tacaaactta ctgaaagaag acatattgat ttattggaac aaggtttaga
    13621 ttttgatgtt ataaatagaa tcattggagg agaattagaa agttgcattc aaaaatacat
    13681 aaaacaagtt aaaaaagttt ctattagtaa caatcttcca gatataagtc caattgttgg
    13741 aaaatatatc gtagagctaa cttatgaagt aatcaaaata gcatcacaat acctaagttc
    13801 actagaacca aatctacatt tatgtttagc agttcaccta aaagcagctt ttgagagatt
    13861 aaaagaagga aaagtaatat caaatgctca tttgaaagaa ataataggaa attatccagt
    13921 agaatattct gtagcagaaa aggtagctca atatttaaag gaaagatata acataaactt
    13981 accaaaagaa gaaataggta taatagcaat atattttaaa atgagtagta gaagagatat
    14041 gtctgaacaa aaaaagatag gcgtacttct tatggctcac ggaagagtag catcttctgt
    14101 atgtgaggtt acaaataaac tattaggaat taatcacgca aggtatttag atatgccatt
    14161 tgagaagaag ccggaagaaa tgattgaaga agctactaca ataataaaag atattgatga
    14221 aggtagaggt gtgattatat tagtagatat gggttcactt tcatattttg gagatattat
    14281 ttctggaaag actggaatta atataaggac aatatctaga tttgatacat tgctttctat
    14341 agaggttata cgaagagcag ttttaccaga tttaacaata gacgagatag ttgatgaagt
    14401 taaaggtata gagccacaag taaaagaaaa ggttagtaaa tgtaacaata caaaagtaga
    14461 ttttagaaaa cctataattt taacattatg tattactgga ttagggggag cttgtaagat
    14521 aaaaaaaatc ttagaggaaa atattaaaag tataactgaa aaggttgata tagaacctat
    14581 aggtatgatt gaatatccaa gtcttgattt agagataaaa caaataagca atgataaaaa
    14641 tgtaatcgcc atagttggaa caattgaccc tcagatagca aatatccctt tttttagtct
    14701 agtagacata aatgataaag caaagttaaa tcaaataaat aacctaattg atacaaatca
    14761 aattggtgaa acaaataaaa aatatgaatt atcagattta atacataaag aagatatatt
    14821 tgtggattta caggttaaat caaaagaaga agttctaaaa tatatgagta aaaaacttag
    14881 agagaaaggt tatgtgacaa aaaatttcta taaaaaagta gtggaaagag ataacttatt
    14941 tccaactgaa ttatcagatg aaatagcaat acctcacaca gatggaatag atgtaattag
    15001 gccagctata agtatagcca tcttaaagaa gcctattgtg tggagtaaaa gtaaagtaaa
    15061 aataatactt ttattagctg tagaccaaaa gtgttttaat ccattaagca ctctactctc
    15121 atttatagaa acagatgaat ttaagaagat aaaaaacatt aaagatagag attttattag
    15181 ggagatgata ttagatggag tcaatgaaaa taattagtag caatttaatt tttaaaaata
    15241 ttgaagtgtc taacaatgaa gatgcattaa agtttttggg acaacgtttg tttgacgaac
    15301 agtatgtaaa agaaagctac atacaagctg ttgtagcaag agaaaaaaag tatgctacag
    15361 ggttaccaac agaaatatac ggagtggcaa taccacatac agatatagta catgtaaatg
    15421 agccaggaat agcaattgga atactgaata aacctgtaaa atttataatg atgggtacag
    15481 acgatactga aattgatgta aaagttgtat ttatgttagc agtgaaagag ccacaagaac
    15541 aattgcaact attagaaaaa cttatgacta tatttcaaga taagaatata ctaaataata
    15601 ttgttgactt aagtgaagaa agtgtgagtg atttattaaa ctctaaattg aaaaattaaa
    15661 catgtaaagt atattaaaaa tgtaaaatta aacatgcaaa gtatgttaaa aaatataaaa
    15721 agattattaa agatttctaa ctatagatat ctttaaaata aaataatttc aatgggggtg
    15781 actaatgtgg ctaaaaagat attagttgca tgtggaacag gagtatgtac ttcaacaatt
    15841 gctataaaca aattaaagaa agcacttcaa gacattggta aattggatat ggttaatatt
    15901 acacaatgta aagtagtaga agttgcatca aaagcacctg attatgattt aataatttgt
    15961 actacacaag tatctagtag tataaaaaca ccagtgataa atggattacc ttttttaact
    16021 ggtgtcggaa tggataaatt aatcaatgat gtattagaag agttagaatt ataattaaaa
    16081 aaattaagga acatggtctc taaaatcata agttttagag acttaaaatt aaataatgga
    16141 gggtctttat atggagatat taaatttcat tgttggtcta ggtgcacaag taatgatgcc
    16201 aataataata tgtatttttg gtttgatatt aggtacaaaa ctaggaaaat ctttaagagc
    16261 tggtttaact gtgggagttg ggtttatagg tcttaatact attatagctt tgctaacaga
    16321 taatttaggt ccagctacac aacaaatggt taagaattta gggctaagtt tatcaataat
    16381 agatgtagga tggccagcag catcagctat agcttttgca tcaacagtag gggctttaat
    16441 tattccaata ggtcttgtgg taaatatagt tatgctaatt actaatacta ctcaaactgt
    16501 tgatgtagac atatgggatt actggcattt tgcttttaca ggagctttag tggcaggggc
    16561 tactcaaagt gtaatgtggg gagtatttgc agcagtagca aatatggtaa ttgtattggt
    16621 aatggcagat ttaactgctc caggtataga ggaatattta ggtatgccag gtatatctct
    16681 tcctcatgga tttacacagg cctttgttcc aattgctata gtagtaaata aattattaga
    16741 tctaatacca ggtataaata agattgagat aaatgctgat acattgcaga aaaaatttgg
    16801 attatttgga gaacctttaa taatggggtc tgtaataggt gtaataattg gtattgctgc
    16861 gaaatatgat ataaaaggaa tattacaatt aggtgtaact atgggagctg tattaatatt
    16921 aattccaaag atggcagcat tattaatgga aggattatta ccagtatcag aagctgcaca
    16981 ggaatttatt gagaagagat ttaaaaacag aggcaagatt tatataggct tagattcagc
    17041 agtaggaata ggtcatcctg ttacattatc agttgcacta gtgttggttc ctttgactat
    17101 acttatagca gctatattgc caggaaataa agttttacca tttgcagatt tagcagtaat
    17161 tccatttgca cttgtattaa tcgtaccaat tacaaaggga aatgtgttta gaacattaat
    17221 aataggaata attataatta cttctggatt attaatcgca actaacttag ctccattatt
    17281 tacccaaatg gctttaaatg ctagttttaa aatgcctgaa ggagcaacta tgatttcaag
    17341 tatttgtgat ggtgcgaatc cattaagttg ggtatttgta aaagttatga actataaagt
    17401 tattggagga gtagtttttg gagttatagc tttaggaatg gctatttaca ataggaatag
    17461 aataataaat gaaaataaaa agctatctat agaagaataa ggttaaaaca attttattaa
    17521 ggagtgtata ggttatgaaa aaatataaat atgattctaa aagatactta ctcagtgtta
    17581 caataccagg attttttatg attttaatat tgatttatgc actgtttaat aactatacaa
    17641 attttaattt gaatatatat agtttgttaa taattgtctg tacttatagt atattcaata
    17701 cttttatatc atcaagtaat cctaaaaaaa ttattattga tagcaaatgt atacatttta
    17761 cctcatttat gactacacat aagtatgaac ttaaagatat agaaaagata agaataagag
    17821 aattttataa taaaaaaatt tatctaaaaa ttaatgatgg aaaccttttt aaaggtaggt
    17881 attggataag aacaaatatg tttaataata gtatagcatt atatagctat ttcattgagt
    17941 tggaagagtg cttatatcca gattcactaa aatttagaaa taaaaaattt gagaataaga
    18001 acatataaaa ataaaaaatt gaattgaagc ctagcttaat cttttttgat aatatataag
    18061 tttgaacttt atactaaatt tttagaattt tcaatcaaga taataacata atagctaagc
    18121 aattagaaca caattaagat gtataaggca tgtatatgat gaatgggagg atttaattaa
    18181 tgttattaga gaaattaaga aaagaagtat tacaagcatc tttagacctt ttaaactata
    18241 atttagtgac attaacagga ggaaatgtaa gtgggagaga tgaacaaact ggctatatag
    18301 caatcacccc aagtggaatg gattatagaa atcttacacc aagtgatatt gttatagtag
    18361 atgtagatgg aaatattata gatggaaaat ggaaagcatc agtggattta tcagaccact
    18421 tgtatatata taagcataga gaagacataa atagtataat tcatacacat tctacatact
    18481 caagttgttt tgctatttta aatgaaccta tagaatgcgc aagtacaact cttgcaaatg
    18541 aagtaggggg ttcagtacca gttgcaaaat tttcaccacc aacatctaaa aagatgggaa
    18601 aatgtgttat tgaagcaatt ggtgacaaaa gagcatgtct tttagcaaat catggcgtaa
    18661 ttgcagttgg tccatctgta ggacatgctc ttacagccgc tgtaatgtta gaagattcag
    18721 caaaagtata ttacttagca aaatcaattg gcacaccagt gctactgcct gatgaagaaa
    18781 ttcaaagagc gagagatgta ttttttaatg tctatggtca ggataaataa attagataaa
    18841 attttaaaag taatatgaaa atatagatat tcaatgatat taaggagatt tataatgata
    18901 taataagttt aatagttaag aagtaaaata tgaaatgtaa tagtatataa attcatttaa
    18961 gactaaaaag aataagataa aataaaaagg agtctgtatt tctatgtatt acttattaaa
    19021 caaaagcaca aatccttact ttaatttggc attagaagaa tatctatttt taaatgataa
    19081 atataatgat gatattataa tcatatggag aaatgaagaa tccattttta taggtaaaaa
    19141 tcaaaatcca tatcaggaag tatatcatga tgttattgaa aagggagaaa ttcctatttt
    19201 aagaagaata tctggaggag gaactgttta tcatgactta ggtaatatca atatgtcatt
    19261 catacaaaaa gataggcaac ttcatgagat agattttttg gagcatacta agtttatgca
    19321 aaagatgctt tcaaccttag gactagatgt atctataact gaaagaaaag atttattttt
    19381 aaatgggaaa aaaatttcag gttctgcaca gtctataaaa agaaaaaatt ctctttatca
    19441 tggaactctt ctttacgatt cagatttaaa taaattaact aaatatttaa attcaaataa
    19501 agcaactgaa tcaaatgcaa ctaaatctgt ttcaagtaaa gttacaaata ttaagttttt
    19561 actcgaaaaa gatataaatt actttttaga ttattgtgtt gaatatttaa aatctaatat
    19621 aaagaatata aaagaacttg aattggatga agaagatatt aaaagtgtat acaatatagg
    19681 agaagataaa tataaaaaga tagagtggac atatggtaaa acgccgaaat ttcaagtagt
    19741 gctaccattt aataatgaaa gtaaaataaa tatacatgtt aacagatgga aagtatcaaa
    19801 attttttatt tcttatgatg ataatgtaat taatatggac aagttgttag atttgaattt
    19861 ctttaaagaa gaaataaaag agagtatttt agaaaattat ccacattaca tagatttaat
    19921 caatttaata ttttaatata aaacctctaa tttgataaaa ttgtcttata tcaaactaga
    19981 ggtttttaat attcttgcat attaacaacc attacaaaat tgtaaaatta aaaacaacac
    20041 tattgaaaaa tcaatatata tttgtttaaa tatatgtgtt tttcattagc aaatagacat
    20101 taactattta tacaactgaa aatttaatat ataattaatc agatggttaa aataaaaata
    20161 ttaaaagttt taataaatga tggttaaaaa taaaatacga ggtgatacca tgtatagtgt
    20221 tataattgtt gcagcgggaa gcggaaggag aatgaactta gatataaaca aacagttcat
    20281 aaaattaaga gaaaaagaaa ttattgctca tacaattcaa gttttttatg aaaatataaa
    20341 tatagatgaa atagtggtat gtataaaaaa agaagaagaa gattttttta aagaaaatat
    20401 aataaataag tataacttta aaaatataaa aatagcatat ggcggaaaag aaagacagga
    20461 ctcaatatat aatggtttaa aaaaattaga taaaaattgt gatatagtac ttatccatga
    20521 tggagcaaga ccatttgtcg accatagaat aatcaacgaa tctataaagg tagctaaaga
    20581 aaaaaaggct gtggttgtag gtgttccagt aagtgatact ataaagatag tgtcagatgg
    20641 aacagtaaaa gaaactccag aaagaaattt gctgtgggca gcacaaaccc ctcaaacctt
    20701 tgaatataat ctcataatag atgcttatga acaagcatat aagaacaatt attatggtac
    20761 tgatgattca atgttggtag aaaatatagg tcaaagtgtc actatggtaa tgggttctta
    20821 tgaaaacata aaaataacta gtccagaaga tttaaatata gcagaacaaa tattaaatat
    20881 ggaaaaaaga gatgaagtta acagtagaag gagaattata tgagaatagg attaggatat
    20941 gatgtacaca aattaacaga ggatagaaaa cttataatcg gtggtgtaga gataccacac
    21001 gataaaggtt tgttgggtca ttctgatgct gatgtattga tacatgcaat aatggattct
    21061 attttaggtg ctctagcatt aggagatata gggaagcatt tcccagatac agatgaagaa
    21121 tataagggag cagatagtat gaaattatta gaacatgtgt ataatctaat aacaagtaaa
    21181 ggatataaaa ttggtaatat agacagtact ataattgctc aaagccctaa gatggctcca
    21241 tatattgaga gtatgagaag taatatatca aaagttttaa acacagacat tgataatata
    21301 aacataaagg caacaacaga agaaggtctt ggttttacag gagcaaaaca gggaatagct
    21361 tcacaaagta tttgtttatt attgttgaca agtcagaata attaatatat tattagtaat
    21421 aagtttaatg gatatcaagt aataggccta tatagaatat ctataatgta tgaatttaag
    21481 tgaaattaat ataaatataa aattagctat gataaagaaa tagtaaaatg atgaaatctt
    21541 cacagagaga aaacggtggt gagagttttc aagaggaagt ctttgaacct gtcttttagc
    21601 ttaaaggtat aaaaatcttt acggtaggaa ccgttatatt cccaaaagag agctattcat
    21661 atagctaact agagtggtac cgcggaaatt aaacctttcg tctctatact tgtatagaga
    21721 tgagaggttt tttatatttt caggaagtta atattcagga ggaatctatt aatgaaaatg
    21781 tcaaaaatgt ttatgccgac attaaaagaa atcccagcag atgctgagat tacaagccat
    21841 caattaatgg ttaggtcagg catgataaaa aaaatgactt caggtgttta taaccaatta
    21901 ccaatggggt taagagtgtt caaaaaaatt gaacaaataa ttagagaaga attaaataaa
    21961 aaagattgtc aagaaatact ttgtgcagcc ttacttcctt ctgaattatg gaaagagtct
    22021 ggaaggtgga ctgctatggg tgaagaaatg tttagactaa aagacagaac agaaagagag
    22081 tactgtcttg ggccaacaca tgaagaagca ttcactgata taataagaca agaaattact
    22141 tcatataaac aattgccact gaacttgtac caaatacaag taaaatatag agacgagaga
    22201 aggccaagat ttggtgttat gagaactaag acttttacaa tgaaggatgc ctatagtttt
    22261 gatgtagatg ataaaggttt agataagtca tatcaagata tgtttgatgc atatgtaagt
    22321 atatttaata gatgtggatt ggagaatagc cctgtacagg cagattcagg agctatagga
    22381 ggttctacat cagccgaatt tatggttaag tcagaagttg gtgaagatga agttgtattt
    22441 tgtagtggat gtgattatgc agccaatgta gaaagagcag aatcttgtaa tctagcatca
    22501 caaaaagaag aaatgaaaga attagaagag gttcatacac caggagcagc tacaataaaa
    22561 gagttagaag aatttttaaa aacttctcca gataagtttg ctaaaacttt agtatatgag
    22621 gcagatggaa aaacagttgt agtcgttgta agaggagata gagaagttaa tgaaatcaaa
    22681 gtatctaatg ccataggttc tgttatagaa tttgctcttg ctacagatga tgtagtgaga
    22741 aaagtaacta atgcagaagt aggatttgca ggtccaatag gtataaatgc tgactatgta
    22801 tttatagata aagaaattgt tgaacaaaga aacatagttg ttggggctaa taaaactgag
    22861 taccatataa aaaatgctaa ctatggaaga gattttgaag gaatagttgg agactttaga
    22921 aatgttcaag aaggcgacaa gtgtatagta tgtggtaagc cacttgaaat agctagaggt
    22981 gttgaagtag gacatatatt taaactagga actaagtact cagaatctat gaatgcaaac
    23041 ttcatagaca aagatggaaa atcaaagcct atagtaatgg gatgttatgg aataggtgtt
    23101 gaaagaacag cagcagctat aattgaacaa cataatgatg agaaaggtat aatatggcct
    23161 ttgtcagtag ctccatacca tgttgtgata gttccagcaa atatgaaaaa tgaagaacaa
    23221 atatcaatag ctgaaaatat atataatgat ttgcaagcta tgggtgtaga agttttatta
    23281 gatgacagag atgaaagaat aggtgtcaaa ttcaatgatt cagaattaat aggaatacct
    23341 atgaggatta cagtaggtaa aaatattaat gaaggtaaag tagaatttaa acttagacat
    23401 aaagaagata aagagataat tgacatagaa gaaataaatg aaaaagtaaa ggcagagttt
    23461 atcaggaata atgttaggtt aggataataa cttaaataag atattgtata gataatcaaa
    23521 gttgtacttt atagacacca ttttatctat atataaggat ataaaatggt gtttttttat
    23581 aaaacaaata aatgaggagg taattaaaat ggcaaaaaat gaaaaacaat ttgttgaaga
    23641 aataacaaag atggaagatg attttccaca atggtacaca gatgtaataa ctaagactga
    23701 cttagttgat tatgcaccag taaaaggttt tatggttgta aagccatatg gatatgcatt
    23761 atgggaaaag atgcaagaat ttatggataa aaaatttaaa gaaactggac ataaaaactg
    23821 ttattttcca ttattaatac cagagagttt attaaacaaa gaagcagaac acgttgaagg
    23881 ttttgctcca gaagttgcat gggtaacaca tggaggaaat aaaaaattag aagaaagatt
    23941 atgtgtaaga cctacttcag agactataat atgtacaatg tatgcgaaat ggttaaaatc
    24001 atatagagaa ttaccatatc tttataatca atggtgctca gttgttagat gggaaaaatc
    24061 aactagacct ttcttaagaa catctgagtt tttatggcaa gaaggtcata cattacatga
    24121 aacagcagaa gaagcacaag aagaaacaat acaacaatta gaagtatata aagcgctatg
    24181 tgaagaatta cttgcaatgc cagttgtagc tggtcaaaaa agtgaaagtg agaaatttgc
    24241 aggtggtgaa agaacatata ctatagaagc tatgatgcat gatggtaaag cattacaatc
    24301 aggtacaagt cacttcctag gtcaacactt tactaaggct tttgatatta cttttgcaga
    24361 tagggaagga aatttagcta atccatacca tacatcttgg ggagcatcta caagacttat
    24421 aggtggactt ataatgactc atagtgataa cagaggttta gtattaccac caagagtagc
    24481 tccaattcaa gttgttatag ttcctatagc tgctaaaaaa ggaaatgtaa tggagactgt
    24541 tgataaaata tatgctgatt taaaagctaa gggagtagct gtagaagttg atgatagaga
    24601 taactataca actggatgga aatttaatga gtgggaaatg aaaggtgttc cagtaagagt
    24661 tgaaattgga ccaaaagata tagaaaataa tcaagctatg gtatttagaa gagatacttt
    24721 agaaaaggat tctatgcctt tagaaggatt agctgatgct atatgtgatt tatttgatgt
    24781 tatacacaat gatatgtttg aaaaagcaag aaaacataga gaagacaata catctatagt
    24841 tgaaaatatg gatgagttta gaaaagcttt agaagaaaag ccaggattca taaagactat
    24901 gtggtgtgga gatgcagaat gtgaagctaa gattaaggaa gaaacaggag caactataag
    24961 atgcttacct tttgaacaag aaaacttagg tcacaagtgt gtgtactgtg gaaaagaagc
    25021 agatagtatg gtagtaatgg caaaagctta ctaaaataca tttaaataaa aactggggta
    25081 gaaagtattt aatactttat cccagttttt ttgtttttta tgagtaataa aatgtatttg
    25141 taactatgtc ataagataag tcatagatac ttatattaaa aagaatataa tagagaacta
    25201 tttttaataa ttacatatta atgaaagcaa aatattttgt tatatagatt ttcaataaag
    25261 caaacaataa atataaatgc atattaataa ggagtgcttt taatttttag aaaataaatg
    25321 acttaaaaga aagatttatg aaattaaaac taggattttc tgatataata aacatgtgta
    25381 tttttaaata aaaaggatat tattattaga tttgattaaa aataaacttg caaaacaatg
    25441 tactagttta atattatagt tatattgaat aaaatatcag gaggaagtaa aaatgaatgt
    25501 aagagtaagg tttgcaccaa gtccaacagg atttgtacat ataggtagtt tgagaactgc
    25561 tttatataac tatttatttg caaaaaaaat gggcggagaa tatatattaa gagtggaaga
    25621 tacagaccaa agtagattag tagaaggtgc tatagagaat atgcttaatg ctatgaaatg
    25681 ggctggggtc aaccatagtg aaggtgttat attagatgat agtggaaaag tagtacaaaa
    25741 aggagaatat ggtccatata ttcaatctca aagattagat atataccaag aatatataaa
    25801 gcaactttta gatagtggaa aagcttatta ctgtttctgt acaaaagaaa gactggatga
    25861 agtaagagat gctcaaagag cagcaggaga aacagctaaa tatgatggtc attgtaagaa
    25921 tctttctaaa gaagaagtag aagcgaatat aaaagcagga ataccatatg ttataagatt
    25981 aagattgcca gagaatcata caattaagtt tacagactta gttagaggag atatggaatt
    26041 taatactaat gacttagatg accaagtatt aatgaaaaca gatggatttc caacttatca
    26101 ttttgcagta gttgttgatg actatttgat gaaaattaca catgttataa gaggagaaga
    26161 atgggtatca tctactccaa aacatgttta tttatatgaa gcttttggat gggaagcacc
    26221 tgtatttgtg catttaccaa atatacttaa taaagaaaag aaaaaattaa gtaaaagaca
    26281 aggtgatgta gctgttgaag actttaagaa aaaaggatat ttaccagagg gattagtaaa
    26341 ctatgtagca ttagttggtt ggtctcctga agataatcaa gaattgttta caatggagga
    26401 attagaaaaa gccttctctg tagaaagagt atctaaaagt ggtggagtat ttgatactga
    26461 aaaattaaat tgggttaatc agcactatat aaaagatggt gatgatgctt atctaacaga
    26521 cttagcaata ccatttttaa tagaagatgg atttataaca gaagaagaag ctactaataa
    26581 atatgatttc ttaaagagta tgatatcagt acttaaagag aaattacaat atgtaaaaga
    26641 agttactgaa catgcaagta tattttttgg agataaaata gaggtagaaa ctgaagaggg
    26701 aagagagttc ttaagattag aacatatacc tactttaata gatgctttga gagaaaaaat
    26761 agaaaaaaca gaggtattaa atgctgagtt tgttcaagct atgcttaaag aaatacaaaa
    26821 agagtacaaa ataaaaggga aaaatttatt tatgggctca agaataattt tgacagggca
    26881 aatgcatggt ccagatttac caaaagtaat ggaggtttta ggaaaagaaa cttgcttaaa
    26941 tagaatagct tatgtaaaaa ataatatatt atagtcataa aagtccgcta atttagaagc
    27001 ggactttttt tgtgttagta tttggtgaaa atatctgctt aagaaaatga aggtatttaa
    27061 ggtaataaat ttaatatata aaaagcaaag caaaaatata aataaaatat gaataataaa
    27121 ctaataaaat attaagcata gaagaaggga atctattatt ttagtttgat ttaattatta
    27181 agggggtaat tactatgtat agtaatattg atgatgttaa aaaggaatta aaggagctat
    27241 gcttagaata tgttacaatt ttagaaaaat taaaagatga aaaaatgatt acagaagaaa
    27301 cttttgaaaa gtgctcttct caaaaaaaaa tatttttaga agaacaatag aggatggtaa
    27361 tagtattatt attattatgt agtttgaaag atagtacgaa aaatgatagt aatactatta
    27421 tttattgcaa ttttcaacta taatataata tataatgtta agaagataat ttataaaaat
    27481 acagtgaggt gatgagtgtg aaagtttata atacattgac tagaacaaaa gaagaatttg
    27541 ttccattaga agaaggaaag gtaaaaatgt atgtgtgtgg tccaacagtt tataactata
    27601 tacacatagg taatgcaaga ccatttataa tatttgatac tctaagaagg tatttagagt
    27661 atagaggata tgatgtaact tatgtacaaa actttacaga tgttgatgat aagattataa
    27721 atagaagtca tgaggaggga atatctcctg aagaagttgc ggctaagtat ataaaagaat
    27781 attttgtaga ctgtgatggg ctaggaataa aaagagctac tgttcatcct caggttacag
    27841 ataatataca acagattata gagtttataa aagagttgga agataaggga tatgcatatg
    27901 cagtaaatgg tgatgtatat tttgatacaa ataagtttga aggttatgga aaactttcag
    27961 gtcaaaaaca agaagattta gaggcagggg ctagaattga ggttaatgac caaaaaagac
    28021 atcctatgga ctttgttctt tggaaggcaa aaaaagaagg tgagccagga tgggatagcc
    28081 cttggggaga aggtagacca ggatggcata tagaatgttc tgttatgtct aagagatatt
    28141 taggtgaaac aattgatata catgcaggtg ggcaagattt aacattccca catcatgaaa
    28201 atgaaatagc tcaaagtgaa gctagaagtg gaaaaacatt ctcaaaatac tggatgcata
    28261 atggatatat aaatataaat gatgaaaaga tgagtaaatc aaaaggtaat ttctttacag
    28321 taagagatat atcaaaatta tatgatttag agatagtaag atttttcatg ttatcagcac
    28381 attatagaaa tccagtaaac tttagtgatg agatgttaaa tcaagctaaa gcaggtcttg
    28441 aaagactata taatactaaa gaaaaattag aatttacgct aagtaattta gtagaatctc
    28501 ctttaacaga aaaagaagta gagcttgtta aagaattaga tgactttaga caaaaattca
    28561 tagatgctat ggatgatgat gttaatacag cagacgcagt aagtgtaata tttgaattag
    28621 ctaagttaat aaattctaat gtagatgaaa attcttcttt ggagtttgct aaaaaatgct
    28681 tagatgaatt taatgaactt acaggagtat taaatattgt aaataagaaa aaggatactg
    28741 tattagataa agacattgaa gaattgatac aaaaaagaac tgatgcaaag aaaaataaag
    28801 agtttcaatt agctgatgat attagacaac aattactaga tatgggaatt gtattagaag
    28861 acacaagaca aggtgttaag tggaaaagga tataaaaatg gaaaaaactg aattagttac
    28921 tatgtcacca ttagttttag catacttagg tgatacagtg tatgagacat atataagaga
    28981 atatttaatt agacagaata cacaaagaaa agttaatgat ttgcataaac tagctataaa
    29041 gtatgtaaaa gctaaagctc aggcaactat aattcatgaa attgaaattg agttaactga
    29101 agaagagagt aaaatttata aaaggggaag aaatcaaaag tcaaatactt ctcctaagaa
    29161 tgcagatatt atagactaca aacatgcaac tgggtttgag gcattagttg gatatttgta
    29221 tttaaataat gaaatagaga gacttcaata tattatcaat aaaggaatca aaattattga
    29281 aagagatatg taaatattaa agagtgttgt cttaataata agtaataagg caacactttt
    29341 tttataacta atgtataata aatatataga aattagtaat gagaaaggat aaaaagttat
    29401 gaaagtaaaa ttaatatcac atacacctga accagaaaaa gtaatagcta tggcagctaa
    29461 gctatgttat tcaccagttg ggacagacga aatagaaaaa gacttaacag atgaaagtat
    29521 agagaaattt ttaaatatgt tattaagtat aggtcatggg tcaatattag aacatgcttc
    29581 atttacattt tcaatagaag gtatttcaag ggcttgttca catcaaattg ttagacatcg
    29641 tatagctagt ttttcacaac aaagtcaaag atatgttaaa ttagaacaat ttgagtacat
    29701 aattcctcca gaaatagaaa aaatagaaaa ggcaaaagag ttatttatag actccatgaa
    29761 aaaagaccaa gagaattatg ataagttagt tgaaatattg tttgaaaatc attataatga
    29821 cctaataaaa aacggtaaaa atgagaaaac agctaaaaga caagctgaaa agaaagcaat
    29881 agaagatgct agatacgtat ttccaaatgc atgtgaaaca aaaatggtat ttactataaa
    29941 tgctagaagt ttgtttaatt tctttgaaca tagatgctgt gaaagagcac agtgggaaat
    30001 aagaaattta gctgtggaga tgttaagaga ggttaaaaag gttgctccta tattatttaa
    30061 aaagactggt ccaagttgtg taaatggaag ttgtccagaa ggaactatga cttgtggaga
    30121 tatagttcaa gttagagaaa aatttaaggc cttgtaggag gtgaataatt tggcgagtat
    30181 agaaggaaga aatccggtaa tagaagcaat aaaaagcgac agagaaatag ataaaatatt
    30241 aattgcaaat tcagctaaag aaggttcaat taagaaaata ataggaatgg ctaaagataa
    30301 gaatataatt attcagtatg ttgataaaca taagttagat gaagtaagta caagtcattc
    30361 acatcaaggt gtaatagctt atgcaagtga gtataagtat tatgaattag atgagctaat
    30421 agatttagca aagaataaag atgaagaccc atttttcatt atattagatg agataacaga
    30481 cccacataat ttgggttcaa taataaggac agcagatgca gtaggagcac atggagttat
    30541 cattccaaaa agaaggtctg tacacataac tcctgtagtt gccaaagcat ctgctggagc
    30601 agtagaatat atgccagttt gcaaggtaac caatatagtt aatacaataa aaagactcaa
    30661 agaagaaggg ctgtggattg ctgcagctga tatggatgga gaaacttttt ataaacaaaa
    30721 tcttactggt cctcttggtg tagtaattgg aagtgaaggt tttggaatat ctagattggt
    30781 taaacaaaac tgtgacttta tagtaaaaat gccaatgata ggtaatgtta catctttaaa
    30841 tgcatctgta gcaggtggaa tacttttata tgagatattc agacaaagac tggataaaag
    30901 taaataggca tgaaaaaaaa tataaatcat tacttaataa tagatggcta caatattata
    30961 aatgcgtggg acaatttgaa agaactggct aaagaggact tagaagattc tcgagaaaag
    31021 cttattgatg acattataga attttctgag tttatgggtt ataagactat aatagttttt
    31081 gatgcttata atgttaaaaa ttctcgagaa aaagttgaaa aaagaaaaca cataacaata
    31141 gtttatacaa gagaacacca aacagcagat agttatatag agaaatttat aacttcttta
    31201 tcaaagtatg atgatgtaaa agtagccact aatgactatg cagaacagca aatgatttta
    31261 ggaaaaggtg ctactagaat gtctgcaaga gaattaaagt tagagttaga tcgttccaag
    31321 aataaaatga aggaaaaaaa tatcggtttg aggaaaaaaa ttcagcgaaa ttggttggag
    31381 gaaaggttag ataaagaaac attgtcgaaa cttgagaaca ttcgtagaaa gcgttgaaat
    31441 tactaggttc tttagactat aatataccta taaatttgtt ttatatatgg gggagattta
    31501 catgttggta gcaaaagaaa aaagttatga gttagtggac aattgtcagc aggatgagta
    31561 taacattgta ttaagagcaa gtgaagggga taaaatagca ctagagtaca ttattactaa
    31621 atacagaaat tttgtaaagg caaaagcaaa gtcatatttt ttaattggag cagataaaga
    31681 agatataata caagaaggaa tgataggcct ttataaggca gttagagatt ttgatggaag
    31741 taagaccaat tcatttaaat gttttgcgga gatatgtata acaagacaaa taataactgc
    31801 tataaaaact gcaacaagac aaaaacatat acctttaaat tcatatgttt ctttgaataa
    31861 accaatatat gatgaagagt cagacagaac acttttagat attatagcta caagtatagt
    31921 aacagatcca gaagagctta taattagtaa agaagagtta aaaaatatag aatcaaaaat
    31981 gaacgaattg ttaagtgact tggaattaga agtactagaa ttatatttaa atggaaagtc
    32041 ataccagttt atagcggaca agcttaaaag agatgtaaag tcaatagata acgctttaca
    32101 aagggttaag agaaaattag aaaaacatct tgaaaacaga aatgattaat agtaaaatac
    32161 ttaccataag cttattgtga gcaaaattaa tttatatcga aagataaggg aggaaattca
    32221 aaatggctaa agctaaatac gaaagaacaa aacctcatgt taatataggg acaataggac
    32281 acgtagacca cggtaaaact acattaacag cagcaataac aaaaacatta tatgacagat
    32341 atcaattagg agaagcagta gatttcgcaa acatagataa agctccagaa gaaagagaaa
    32401 gaggaatcac aatatcaaca gcacacgttg agtatgaaac accaaataga cactatgcac
    32461 acgttgactg cccaggacat gctgactacg ttaagaacat gataacagga gcagcacaaa
    32521 tggacggagc aatattagtt tgttcagcaa cagatggacc aatgccacaa acaagagagc
    32581 atatactatt atcaagacaa gttggagtac catatatagt agtattctta aacaaatgtg
    32641 acatggtaga tgatgaagag ttattagagt tagtagagat ggaagtaaga gatttattaa
    32701 cagaatatga tttcccagga gatgacactc caatagtaag aggttcagca ttaat
//
LOCUS       DAELNY010000023        32646 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.023, whole genome shotgun sequence.
ACCESSION   DAELNY010000023 DAELNY010000000
VERSION     DAELNY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.023"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1675)
                     /locus_tag="KPE55_000965"
     CDS             complement(<1..1675)
                     /locus_tag="KPE55_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG0492061.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            1892..2629
                     /locus_tag="KPE55_000966"
     CDS             1892..2629
                     /locus_tag="KPE55_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0492062.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(2746..3204)
                     /locus_tag="KPE55_000967"
     CDS             complement(2746..3204)
                     /locus_tag="KPE55_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0492063.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(3486..3773)
                     /locus_tag="KPE55_000968"
     CDS             complement(3486..3773)
                     /locus_tag="KPE55_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG0492064.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(3851..4276)
                     /gene="ruvX"
                     /locus_tag="KPE55_000969"
     CDS             complement(3851..4276)
                     /gene="ruvX"
                     /locus_tag="KPE55_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG0492065.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(4482..6653)
                     /locus_tag="KPE55_000970"
     CDS             complement(4482..6653)
                     /locus_tag="KPE55_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492066.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(6663..6920)
                     /locus_tag="KPE55_000971"
     CDS             complement(6663..6920)
                     /locus_tag="KPE55_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG0492067.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(6945..9584)
                     /gene="alaS"
                     /locus_tag="KPE55_000972"
     CDS             complement(6945..9584)
                     /gene="alaS"
                     /locus_tag="KPE55_000972"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG0492068.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDRESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(9652..9890)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10033..11112)
                     /gene="mnmA"
                     /locus_tag="KPE55_000973"
     CDS             complement(10033..11112)
                     /gene="mnmA"
                     /locus_tag="KPE55_000973"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG0492069.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(11401..11841)
                     /gene="nifU"
                     /locus_tag="KPE55_000974"
     CDS             complement(11401..11841)
                     /gene="nifU"
                     /locus_tag="KPE55_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG0492070.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(11895..13088)
                     /gene="nifS"
                     /locus_tag="KPE55_000975"
     CDS             complement(11895..13088)
                     /gene="nifS"
                     /locus_tag="KPE55_000975"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG0492071.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(13106..13552)
                     /locus_tag="KPE55_000976"
     CDS             complement(13106..13552)
                     /locus_tag="KPE55_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0492072.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(13661..14431)
                     /locus_tag="KPE55_000977"
     CDS             complement(13661..14431)
                     /locus_tag="KPE55_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492073.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(14462..15727)
                     /locus_tag="KPE55_000978"
     CDS             complement(14462..15727)
                     /locus_tag="KPE55_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG0492074.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(15834..16619)
                     /gene="codY"
                     /locus_tag="KPE55_000979"
     CDS             complement(15834..16619)
                     /gene="codY"
                     /locus_tag="KPE55_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG0492075.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(16784..18871)
                     /gene="topA"
                     /locus_tag="KPE55_000980"
     CDS             complement(16784..18871)
                     /gene="topA"
                     /locus_tag="KPE55_000980"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG0492076.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNQILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(18904..20004)
                     /gene="dprA"
                     /locus_tag="KPE55_000981"
     CDS             complement(18904..20004)
                     /gene="dprA"
                     /locus_tag="KPE55_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG0492077.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(20023..21624)
                     /locus_tag="KPE55_000982"
     CDS             complement(20023..21624)
                     /locus_tag="KPE55_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG0492078.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSDTECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(21655..21999)
                     /locus_tag="KPE55_000983"
     CDS             complement(21655..21999)
                     /locus_tag="KPE55_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG0492079.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            22183..23346
                     /locus_tag="KPE55_000984"
     CDS             22183..23346
                     /locus_tag="KPE55_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0492080.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENTFILISYFLELILIFLLCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLVISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSITLYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            23339..23986
                     /locus_tag="KPE55_000985"
     CDS             23339..23986
                     /locus_tag="KPE55_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0492081.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            24115..25050
                     /locus_tag="KPE55_000986"
     CDS             24115..25050
                     /locus_tag="KPE55_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492082.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTSFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            25064..26161
                     /locus_tag="KPE55_000987"
     CDS             25064..26161
                     /locus_tag="KPE55_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492083.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKENYKWREV"
     gene            26164..27228
                     /locus_tag="KPE55_000988"
     CDS             26164..27228
                     /locus_tag="KPE55_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492084.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            27472..27969
                     /locus_tag="KPE55_000989"
     CDS             27472..27969
                     /locus_tag="KPE55_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0492085.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(28146..28394)
                     /locus_tag="KPE55_000990"
     CDS             complement(28146..28394)
                     /locus_tag="KPE55_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492086.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(28460..29476)
                     /locus_tag="KPE55_000991"
     CDS             complement(28460..29476)
                     /locus_tag="KPE55_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0492087.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(29534..30301)
                     /locus_tag="KPE55_000992"
     CDS             complement(29534..30301)
                     /locus_tag="KPE55_000992"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG0492088.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(30369..32558)
                     /locus_tag="KPE55_000993"
     CDS             complement(30369..32558)
                     /locus_tag="KPE55_000993"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG0492089.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        10318 a         5736 c         3492 g        13100 t
ORIGIN      
        1 atacttccat tatgatagga agtatcattg gatttcgttt agttttttga tataaatatt
       61 cttttaggtt ttctttaata ttattcttta aataagccca ttctttaata tttttctctt
      121 cacattcatt tagaacattc ttaattatat cctttgctcc atccattaaa tcttctgact
      181 ctcttacata tacgaatcct ctggaaatta tatctggacc tgctaagacc ttaccctcat
      241 ccttggaaat agtaactaca actatcataa gaccatcttc tgataaatgc tttctatccc
      301 ttaaaactat attacctaca tcgcctactc ctagaccatc aactaatatg tttcctgttt
      361 gtatacggtt tgctacctta gcagaatttt tatctaattc taaaacatct cctgtttgca
      421 tgacaaaaat attttcttta tccataccaa gctgttcagc tatttcagca tgtcttttta
      481 acattctata ttcaccatga gctggcataa aaaactttgg tctaactaat gcatgtataa
      541 gttttaattc ttcttgacag gcatgtccag aaacgtgtat atcagctata tcactgtata
      601 ctacttctgc tcctttttca aataagaaat tgattactct tgatattaat ttttcatttc
      661 ctggtattgg atgagcagat attataacta aatcgccact ctttatttct acttttttgt
      721 gctctgaaaa agccattcta gcaagagctg acataggttc accttgtgaa ccagttgtta
      781 ttatcactaa ttcactatct tcatacttac atatatcatt taaatctata agcatgtcat
      841 cagaaatatc taggtatcct aattcttttg caactccaac tacattgacc attgagcgac
      901 ctgatatagc aacttttcta ttaaactttt cagcagtatt tattatttgt tgtaatctgt
      961 gtatgttgga tgcaaaggta gcaactataa ttcttctgcc atttcctttt gcaaataatt
     1021 cgtcaagacc taccccaaca gtcttttcag acatagtaaa tcctggtttt tctgcattag
     1081 tactatctgc cagcattaaa agaactcctt ttttgctcaa ctcacagatt ctatgcatat
     1141 ccataacatc tccatcaata ggagtcaaat caatcttaaa atctcctgtg tggtatatta
     1201 taccttggtc tgtgtgtatt gcaatagagt aagcatctgg tatactatgg ttatttttca
     1261 aaaactctac ttccatatta tcaagcttta taacttgtct aggacctata acatttaact
     1321 taacattatt cattttatgt tctttaagtt ttacttgtat aagacctata ctaagtcttg
     1381 ccccatatac aggtacattt attttcttta atatatatgg aagagctcct atatggtctt
     1441 catgtccatg tgtaataaat atacccttta ttttatccct attttttact aaataagtta
     1501 tatctggtat aactatatct actcctaaca tttcatcctc tggaaaacta agtccagcat
     1561 ctataacaat tatttcatct ttatactcaa caacagtcat attttttcca acttcgttaa
     1621 gtccacccaa ggccattacc tttatcttgt tggtgttctt tttgaacaat tgcatcatct
     1681 ccttctgtct atttaatttt ttaacacgtt ctacaatcat aaataatatc aaatcacgca
     1741 ctcgctacca gttattctta ttgtaccata attcgtatca tattttaagc tatatttaaa
     1801 atacatttaa cgtattattt tgacaaacct attttatttt gatatcctct aaatattata
     1861 tgatttaata tcggataatt taggaggatt tatgcgtggt aaaactcatt gtgcaatagg
     1921 aatcttaaca gctatccaaa catctttaat atttaaaatt cctatatctt tagttgatat
     1981 tttagtttca gctacttttg ctgtactacc cgatttggat aaatctaatt ctatggtctc
     2041 aaatttcatt ctaaaaaaca atgtatccaa atacatatat agaattttta tatatgctgt
     2101 taatattatt attttcttta tttcaataaa tataaatgat aatttttatt taagcgcaat
     2161 aattacattt gttgcaatta taattattga agcaaaacta acccacacat ttctaagaaa
     2221 agtttttcta tcattaatat ttatactatt agctatatgc ttatacatca tagaagttga
     2281 aatatatttt acaatttttt gtctaatgtt gtcaattttt ccctggttaa agcacagaag
     2341 tttttctcat agcatatttg caactataat agtatatttt ttactaaaac agatagaatt
     2401 aatcactaat attaataatt tatcttttta tggtacgatt ggttatgcaa gtcatatgtt
     2461 tttaggagat ttatttacaa agcaaggaat accaattttt tatccgctaa gcgaaaagaa
     2521 aatttcttta ggttttttaa ctgttggagg accatttagt aatttcatag aaaaatcatt
     2581 catttttgta cttataggat taattatatt ttcaatatta aaactataag catcttgaaa
     2641 actgtatatt tgcattttgt agatggttta taataaaaaa tggaagaata gcatagaata
     2701 taaaagttct atactattct tccatcgaca ttaagcaata gaagtttatt cttctgaatc
     2761 gcttactcca ttacactcgt cacatagtcc ataaaattta acatcatgat caagtatttt
     2821 aaatttgtac aatgattgta tctctttttc tatattgtct aaaaggtctt ctttagcttc
     2881 cattatcttt ccacaatttt tacaaataag atgatgatga ttatgacagt cttctttatg
     2941 tagactaatc tcatatctta tacatccatc gtctaagttt agttttgata ttaatcctat
     3001 ttcatccaat agctgcattg ttctatatac agtagctaag cctatttcag gacagtcaac
     3061 cctaactaaa tcatagattt cctcactact tagatgtgaa tggtcatgtt taagcaatat
     3121 ttcaactatt gctcttcttt gtggcgtaat cttaaaacca gtttctttta acttgtcttt
     3181 taataaatcc attgtatttg ccataacatc acccttacta ttttcaattg ataatcttta
     3241 ttaaataata ttgtaattga taattcattt tttgtcaaga catttaaata aatttaattt
     3301 attctcataa tataaatata tacccataaa agatatatta ttcaccaaaa ttcataaatt
     3361 ttttggtatt tttaataaaa aacaaaaaaa cagatatata ctataaatgc ttttacaatt
     3421 aataaggttt aattaaagca aatataataa atatctgatt tttatataca aacttaatta
     3481 cttatctagt taagtccatt tgattcatct tcgtcgtaat ttaaaccttc ttcttccatt
     3541 atagcagtat acacagctac aacagtttca tactcttcat cactttccaa aggtattaag
     3601 atttcacctt cttcatcctc atcaattcta aagattaaag cttcttcagc ttcttcatca
     3661 tctaaaggca ttaatattgc atactctttt ccttctgctt ctaatgttaa tattatttca
     3721 aattggcttt caacgccatt ttcgtctatt aaatttatta tattttcttc cataatgtca
     3781 ttcacctcaa atttattttt atttttatta tttatattat atatcataag tattcaattt
     3841 tatagttctt ttatgaattt attttaaaat ctaaatatcc ttgcaaaatt attactgctg
     3901 ccaacatatc tataaccttt tttctttttt gtctactaac atctgcttct aataaagacc
     3961 tctctgccgc tactgtagaa agcctctcat cccaaaactc tacatcaata cctgtttctg
     4021 cttttaccaa ttcacaaaac ttgattactt tttctccttg tggacctaaa gttccattca
     4081 tattttttgg taatcctgat acaattttat ttacttgttt ttcttttatt attgctttta
     4141 gctcttcaat atcttttttc ttgccaactc tttttattgt ttttacacct tgtgcagtta
     4201 atcccatcaa atcactcaca gcaactccaa tagttttatc accaacatca agacccatta
     4261 ttcttccatc taacatagtt cctcctttat ctatttatat tctatagatt aaatacatat
     4321 tctgaacctc tcataactaa ggtcacaggc ttactgcttc atataatttt acataatcaa
     4381 tcataaaaat tgcaatatga ttttttatgt aagcatccag tttacattaa taatcatatt
     4441 gcatttttta tttaaatcct atatgaacag taaaaatttt tttagttatt tttttcaaat
     4501 attatcatat ttatacttat tattattagt ggtataagta tatatataga tgctgtagat
     4561 gcagcaacta ttccagaatc attaactaaa agagtaatta tacaaccaac catagatgca
     4621 ataaaccctt taaacaagat tggaaaatta ttcattatct ttctaaagtg tctagatggt
     4681 ttaaatataa gcactgcaat tactccaatt cctacaagta aaatatttac ccatacactt
     4741 gtctgtgcta gttgcatatt catttgaatt tttcttccaa atgtttgtaa tatctctcct
     4801 ggtccattta ggaaaattct ctgtacaaac atacctaaat gagattcact acctgaaagt
     4861 atatctaaag ctgcaaatgc aaaaacgaca actacagctg aaacagctaa taatacagct
     4921 ttcttaaaat ctattttaat gtcatatata agcattataa acaacaaata tgctacacat
     4981 tctgatatag ctccaccaac atttgcaccc attgaaggat atgcactagt tataagtatt
     5041 attaatgaaa aaattacaac aagtaattta gataccttct tataatgtaa taatactgct
     5101 aagccaaata ctgcacttgc tatagttaca ccttcatatt catttcctac accatagtac
     5161 ctagctccta tgacagcatc ataactcatt atgttatttt gcattaagta tgttccaaat
     5221 acagaatcta taactatgac taaaatagta agtgttgcaa aaaatcccat gtgctttata
     5281 tcattgtcct taaataattt tctacctaac aagtataata ttagcgtagt cgcaaatatt
     5341 cccaaagata ttgaaattgg agttttaaaa ttcatcaaag gagctaacat aaatgatagt
     5401 ggcattatta tgcctaattt tataaactcc ttaagtactt tgaatacacg ctctttatgt
     5461 ggcatataat ttctaaagaa aatcgcaaac atggcaatta cccaagaagc agatattacg
     5521 cctacaaagc catttataat agttgacctt attgaagata tagacactat cctttcataa
     5581 agtgatttta gatagctaat attgtcctct ttatttacta aagtataact tctacctacc
     5641 atctctttat tttcaagacc aaatgtacct agtatatcta ctccaacatc taaattggca
     5701 acaattccag cctttcttgt tgtggcagat tccagtagac ctttcccttc tccactaaat
     5761 tttattattg gagaaagtct tctcttattc ttataatcta ggtcacttgg aaatgcactt
     5821 gctatataaa cagtgtcatt ttctccaacc atagaaaata cttgttcaag atatttattt
     5881 atatttttta caatattttt cttcattgta tcataagtat tatcatttag atttaatcta
     5941 tacatatcta atctatatgt atctccaagt tctacaaata tagcatcact atttttatag
     6001 gattttttag tttcatctag taatttatca taatctgtag atattccaaa tggcatttta
     6061 gaatctttct tattaatatt atcaatatta cctgaatcaa tccttccata ttggtccata
     6121 gctactaaac ataagtttct gtttttaact aattgattat tttcaattat atcagcattt
     6181 ccaattacag atgtctttaa accattttct gataaagttt ttcctaatga ccctaaattt
     6241 gctccatatt ctccatactc aagattttca tttatagatc tattaatgcc taaatcattt
     6301 atttctttag ctttttgacc tgttgcgact tcaaaagctt tagcagtatc ttggtctact
     6361 ttttgaaaat tgatatagtc ttcagtagtt acactagctc ttgttcctgc acccatactt
     6421 gcataagacc ttctatcatc agtaccttga tcccctctta tattcatgag agctacatag
     6481 cctctctttt ctatctcatt tttcaagatt ggaatattca tcatacttcc aagattggtc
     6541 ctattcatat caataaatat gaccttgcct ttttcttcag caaaaccata gctagcagtt
     6601 gatacaaaaa tcataatgac tattgcaaac aacgacataa tcttttttct catttatttg
     6661 acctactttc tattcagata gtgcgtgatt acttcttcaa gtatctcatc tctttcaaat
     6721 tttttaatta tagctcttgc atttttatga ctagttatat agctagaatc accagaaagg
     6781 agatacccta taatttgatt tatagggtta tatccttttt caactaatgc tttatataca
     6841 aaatcaatag tatctcctac actcatccta tcttctggta ttccttcaaa tttcatagtg
     6901 tagtctaaat ctttttccaa actaaaaacc tccttgagtg aatgttattt aacttgtgtc
     6961 ttaataacct cacttgcata gcttaaagct tggtcaacct tagatacatc actagctcct
     7021 gcttgtgcca tattaggtct tcctccacct tttcctccag ctatttttgc aacttctcta
     7081 acaatattac ctgaatgtat tcccttatct aatacatctt tagtggcagt aactacaaag
     7141 ttaactttat catctactac atttgcaagt actactacac cagaaccaag cttatctctt
     7201 agattatctg ctgtttctct tagtgtatcc atatccatac cttcaaattt attagttatc
     7261 aaattaactc catttacttc aactttagaa tcaaaaatag aatcaactga ttgtaaactc
     7321 atcttagctt tcatatcatg caattcttta gataagtttt tattttcttc taataaagaa
     7381 cttattcttt gaattaaatt atcttcttta gattttaagt ttacacaaac ttcagagata
     7441 attccatctc tttcttttag gtattcataa acagcttttc ctgttattgc ttctattctt
     7501 cttacacctg ctgctactcc accttcagat aatattttga acattcctac ttgagaagta
     7561 tttgtcaagt gagttcctcc acaaagttct gtagaataat ctcccataga aactactctt
     7621 acctcatcac catatttttc accaaatagt gctgtagctc ctttttcttt agcttccttt
     7681 atattcataa tatcacattt tatgtctaaa gatgataaaa taacattatt tactttttct
     7741 tctattactt ttaattcttc atttgatatc gcttcaaagt gagtaatatc aaatctaagt
     7801 ctttctggag ttactaaaga accagcttga ttaacatgtt ctcctaatac ttctcttaat
     7861 gccttatgaa gtaaatgtgt agcactgtga tttcttgcag ctgacattct agtttctcta
     7921 tctacgagtg tctttacttt gtccccatta cttattcttc ctgattttat tatacctata
     7981 tgttttatac tattattagc acctttttta gtatttaata cttctaaaac taagttttca
     8041 ttagttatca atccacaatc tccaacttgt cctccacctt caggatagaa agtagtgtta
     8101 tctagtacta tagatacctt atcgccttct tctgcacttt gaactagctc atcatcttta
     8161 actatagctt caatagttcc ttcaccataa atttcactat acccattaaa tgtactgtca
     8221 actgtagatt ctaattttga tagagggtct tccttccaac tttctctatc catatttcct
     8281 ctagcatttc tagctctttc tttttgcttt tccatttctt cattaaaagc ttcttcatct
     8341 acagatagat gttcttcttc taatatttct ttagttaagt ctatagggaa gccataagta
     8401 tcatatagtt taaaagcttc ttgaccactt aggacagtct ttccttcatt ttttaatact
     8461 tctatataag agttcaatat ttctgttcct tggtctatag tttcattgaa tttttcttct
     8521 tctattccaa ctactttttt aatataactt tctttttcaa ctaaaactgg atatgcttta
     8581 tcatttactt ttataacttc atctattagt ttctgtaaaa ataattcttt tactcctaat
     8641 aacttaccat gacgagcagc acgtctaagg agtcttctaa gaacatatcc tcttccttca
     8701 tttgatggaa gcactccatc actaactaag aatgttacag ctcttatgtg gtcagttatt
     8761 attcttattg atatatcatt ttttggattc tctccatatt ttactcctgt taacttctca
     8821 actgcttcta gtatagattt aattgtatca acttcaaaga tagtgtctac gccttgcata
     8881 atacatccca ttctttcaag acccatacct gtatctatat tcttgttttc taatagacta
     8941 taatttcctt cttcatctct atcaaattga gtaaatacat ggttccaaaa ttctaaatac
     9001 ctatcacaat cacagcctgg tttacagtct ggattgtcac atccatattc ttctcctcta
     9061 tcaaaatata tttctgaaca aggaccacaa ggacctgtac ctatttccca gaaattatca
     9121 tcttttccaa gtctaaccat tctttcttct gggaaattca tttcttttgc ccatatatca
     9181 taagaatcat catcttcttc atatacagtc acccatatct tatcttctgg tatatttagc
     9241 cattctgtaa caaattccca tccccatttg attgactctc ttttaaagta atctccaaat
     9301 gagaagttac ccatcatttc aaagaatgta gcatgtcttg cagttatacc tacattttct
     9361 atatctcctg ttcttataca tttttgagat gtacacattc tcacacttgg aggtacttct
     9421 acacctgaaa agtagttttt caaaggtgcc atacctgagt ttataagtaa caaactctta
     9481 tcattgtttg gtactaaaga ataactattt gctacataat gaccttttga ctcaaaaaat
     9541 tctaaaaact tacttcttat ttcatttaat cccatctttt ccatatcatg ttcctcctaa
     9601 taaattttat tttttcgttt aaataaaaaa cctcgtccct ataaataaat ttataggggc
     9661 gaggacaatc gcggtaccac cctaattatc atacaaagta tgatatcttc ggaaaacctt
     9721 agctataacg taactaccgt caatctctac ttaaatcttc tttataaaat ataaattgtt
     9781 caagattgct actaaaaggc tgcttcaaat atcatattct aagggcttcc agcatttccc
     9841 tctctctgta agacatcaaa tattttacta ttccttatca ctgtatttgt tttttaatta
     9901 atatactaat tatattacta aattttacaa aattaatcaa tactatattt tcatatacaa
     9961 taaatttatt ttataaactt tatttactct taatatactt tattgcttat ttttttaatt
    10021 ttttagatgt ttttataaac ttttctcgat tatacctcca cctactacaa tatctccatc
    10081 atacattact acagattgac ctttagtaat agctctttga gcttcatcaa aaactatttt
    10141 aatagtatct tctcctactc tatgaatagt agctggtgat ggttttgctg aatatcttat
    10201 tttggcttga actcttaagg gttcttctaa cgtatctatt gatataaagt taacatcttt
    10261 agcaataagt tcctttttaa ataaatcttc attgtctcca agaactatgg tattatttat
    10321 tggatttatg tctattacaa acattggttt tccaaaagta attccaagcc cttttctttg
    10381 gcctatagta taatataata tacccttatg ttttccaagt atattgcctt tagtatctac
    10441 aaaaaaacct tcttcaatac gctttttaga atgttttttt acataatttg catagtcatt
    10501 atctttaaca aaacaaattt cttgactatc aggcttattg tgtactgcca tacccatttc
    10561 cttagcaatt tctcttactc tatctttttc atagtcacct attggtagta aagtatgctc
    10621 tagttgctct tgtgtaagat tataaagtgc ataagtttgg tcttttttat ctgtaacaga
    10681 tttttttagc aaatatcttc ctgtactttc atccttttct atctttgcat agtgacctgt
    10741 agctacataa tcacaaccta tttgtctagc cttcttataa aaatcatcaa attttatatg
    10801 tttattacaa gctatacagg gattaggagt ttttccttcc aaatattcat ctataaaata
    10861 atctattact ttttctttaa acacttctct aaaatttaaa acataaaatg gtatacctat
    10921 tttatttgct acccttctag catcttcaac agcagataaa gaacaacaac caccttcaat
    10981 ttcaacaaca tcatcatctt gccacaactt catagtaaca ccaatgacat catatccttg
    11041 ttgttttaaa agatatgcag ctacagagct atcaactcct ccactcatac ctatcattac
    11101 ttttttattc ataaacaaat ttcacctcat cttttgcatt acttattatt ataccatata
    11161 gagcaagtat tgtacttatt taaaatatag cttgttctat tttctaaaaa aataatcatc
    11221 tttattattt gaatcttatg gattattttg ttaactaatt aacctctaaa tcaagttaaa
    11281 atacttaatt taggcaataa aaaatagcca ttttaaaaac agctattttt tatgtcctaa
    11341 ttatatcatt agtacttctt tttaatcatt gtaaacttct cataacacaa tcacttattt
    11401 ttattcttct tcttcaacat catggtcatg agcatcatct ataacatatc catccaattc
    11461 tggtatatgg atattgttct tttgagcata atctataaga gctgctttta cagcttgttc
    11521 agctaaaact gaacaatgca tctttactgg tggtaaacca tccaaagctt ctgcaacagc
    11581 tttatttgta agttctaatg catcttttat agtttttcct tttatcattt cagttgccat
    11641 agaagaactt gctatagctg aaccacagcc aaatgtttta aacttaacat cctttattac
    11701 atctccatct atgtctaaaa atatcttcat tatatctcca catgtagggt ttccaacttc
    11761 acctacacca cttgcattat caatttctcc catatttctt ggattcataa aatgttccat
    11821 aactttatca ctatattgca ttatcttttc ctcctactca atttatgtct attttttatg
    11881 ctttatcttt atgtctattt tactaaacct tgtttcttcg catcatcata aagtggtgac
    11941 atgcttctaa gtctttctat tacttttggt aagttctcta gtatataatc tatatcatca
    12001 tctgttgtaa aatctccaac tgtaagtctt aatgaaccat gtgctatttc atgtggtaaa
    12061 cctattgcaa gaagtacatg tgaagggtct aatgaaccag aagtacatgc agaaccactt
    12121 gaaccagcaa ttcctaacat atctaataat aaaagtattc cttcaccttc tataaattgg
    12181 aatgcaaagt ttgcatttcc tggcaatcta ttttctaagc taccatttac tcttgtgtaa
    12241 ggtattcttt ctaacacacc atctattaac ttatttctaa gacgagttaa agtttctaca
    12301 tgattttgca tattttcctt agctaattct gctgcttttc catatccaac tattgcaggt
    12361 atattttctg taccagctct tttgcttttt tcttgtgctc caccatggac aaagttatga
    12421 agtcttacac cttttcttat gtataaagct cctacacctt ttggtccata tattttatgt
    12481 gaagacatac tcatcaaatc tattcctaat tctttaacat ctactggaat attacctgta
    12541 gcttgtactg catcagtgtg gaataatatc ttatgttttt tagctatttc ggatatttct
    12601 ttaataggct gtatagttcc tatttcatta tttgcaaaca ttatacttat aagtatagta
    12661 gtatctttta ttgaatcttc tagttgcttt aagtctactt ttccttcgct atcaacatct
    12721 aaataagtta tttcaaatcc atgatgtttt gctaagtatt cacatgtatg aagtattgca
    12781 tggtgttcta tttttgaagt tattatatga ttacctttgt ttttattagc ataagctaca
    12841 ccctctagtg tccagttatc actttctgaa ccacctgctg taaaatatat ttcactaggt
    12901 tcagcattta taagggctgc tacttgttct cttgctttat ccaaagcatc ttttgcttct
    12961 cttccgaatg tgtgaaaact agatgcatta ccaaaataat ctgttaggta tggcatcatt
    13021 gcatccaata cttctttttt aataggtgtt gttgcagaat aatccatata caatcttctt
    13081 ttttccatta cattcatctc caaatctatt ttttaatatt tgttatatca ttttctaatt
    13141 tatttttgtt atagtcattt atcatatctt taagagtaat tgaatctata acatcttcta
    13201 tacccttttt catcttttcc caaactattt ttgttacaca catgtttgag ttttcacata
    13261 catcttcatc tagtacacag tcagataatg caactggtcc ttctaatact acaagtatat
    13321 ctcctactgt tatgtcttca gcattctttg aaagcaaata acctccttga gcacctctca
    13381 cactttttac tagacccgat ttttttaaag atgaaaatat ttgttctaag tactgatctg
    13441 atattttttg cttcttagca ataaatttta atgatactgg tccattgtct tgatttaatg
    13501 ctaattcaaa cattgctttt agtccatatc tacctttagt agacaacttc atatattatc
    13561 acctctatat agaaatctaa ttcatactgt tttagtagga attactttca agataatttt
    13621 attataccct actaattttg tcaagattaa ttttaatttt ttattttgaa taaattcccc
    13681 attttccaat gttttcaaat cctagttttt tatatatctt tccagcctct tcattatcat
    13741 aaaacaaaca aggaattttg ttttctctca gtaactctga acatagttta attaggcatt
    13801 ttgtagcaag tcctttagct ctatatttag gatgtgttcc tactccaatt atcattgcat
    13861 gagtcctatt ttctgaagta ctctttgcca ttgacaccac ctgtttgtta atctctatac
    13921 aatatcctct acctgtcctt agtccatttt ttaaattctc taaagttgta ctgtgaaatt
    13981 catctattaa gtcataaagt tttactgtct tttttagatt tccaagccta attctcttaa
    14041 cctttgcatt acaattatta tctatcaaaa actttttagt ttgaagtttg caaaaatcaa
    14101 ctacctttaa attattaagt cctaatctct ttgctaaagc atctacagca catattttcc
    14161 cagatatttc ttcgtaatta gtattacgaa gtagattata aaaaccttca acatcatact
    14221 ctccatcaga ataaaacatc ataaattcaa aatatttaag taatattgcc tttatttcac
    14281 catgttcacc cacatctgcc catatattta aaaaattatt tccatatcca tatctttcaa
    14341 tatcccctat tataaacaaa ttgtactcag gttctttttt taagtatttc ataactctac
    14401 tgtgataagt tgagttaagt tttataatca taatatctac ccccgataca taaagttcag
    14461 tctaaaaatc actatttaga tattctaaat atttttttat atttttctca gttttatttt
    14521 cttgtggtat gtaataaata ctatctttta tgctatctgg taaatactgt tgctttacat
    14581 aatgattttt aaaattatga ggatatttgt atccacaacc cctacctatt tctttagaac
    14641 cagaatagtg agcatccttt atactatttg gtatatcctt aacaaactct ttatccagtt
    14701 catccatagc tttcattatt gctatacatg cagaattaga ctttggagat gtagccaaaa
    14761 gaatagtagc ttctgctaat ggtattttcg cttctggaaa tccaagttgc atagctgagt
    14821 ctacacatgc ctttacaatt acaatagcat ttggataagc taaacctata tcttcactag
    14881 ctattactaa aagtctccta catatactta tcaaatcccc accttttata agtcttgcta
    14941 gatagtgtat agatgcctgt gggtcacttc ctcttataga tttttgaaat gcacttaaaa
    15001 tatcataatg actatcacca tttttgtcat agtttataat tttattaaat gtagatgccc
    15061 ttattacatc tgaatctata taagttatat tatccttatt tggtttagtt gaatagacta
    15121 ccacttctaa aatatttaag gctcttctca tatctccatc agaaagaatt gctatatctt
    15181 caatagcatt attattgcac tcaatatcca tatagctatc ttcatttaat acttcaacag
    15241 ctcttttcag tcctttttct atatcaagtt tttctaaagg tttaaattca aatacagtag
    15301 acctacttaa aagagcttta tatacataat gatatggatt ttctgtagtg ctagcaatca
    15361 aagttatact cccattttcc ataaattcta atattgattg ttgctgtttt ttattaaaac
    15421 tttgtatttc atctatatat aataggactc catttacatt atttatactt ccaagctctg
    15481 atatcacctt ttttatatct tctaaagatg aattagtagc atttatttta tagaagcttt
    15541 tattgcttct ttctgctatt atttccgcta ctgtagtttt accaacacca ggaggaccaa
    15601 aaaatatcat atttggtaaa aaattagatt gtaatatctt agacaatatt tttccattac
    15661 ctattatatg actttgacct attacatcat tcattgtttt tggtcttatt ttatctgcta
    15721 aaggcatttt ctcactcctt tttaatatac ttatcattac cttaataatt cgtctaaaaa
    15781 aggtatctat ttgaaataga taccttttct aatctaaacc tataaaatat agattattga
    15841 ttgtttttta atttttttaa ttcatctgta agtttgtcat taagtattct tatatgagta
    15901 cctttcatac ctaatgatct tgattctatc acacctgcac tctcaaattt tctaagtgca
    15961 tttactatta cagacctagt tataccaact ctatcagcta tcttacttgc tacaagtaga
    16021 ccttcttttc catccaattc agcaaaaata tgttcaactg cttcaagctc ggagtaggac
    16081 agagtgccta ttgccatttg aactacagct ttctttctca tttcttcttc taattcttca
    16141 cctattgctc ttaatatttc aagaccaaca acagttgcac tgtattcagc tattactaaa
    16201 tcatcatcat tgaatgaatt tgaatatctt gaaagtacca atgttcctaa tctttgaccg
    16261 cttcctaata ttggaactac tgtagtatat ttttgtaatc ttccatgttc ttcaggaaag
    16321 atttctaata tcttctcacc atttaaattt tctaatgttt catcaatttt taacacattt
    16381 tgagtatatt catctgaaaa tttcttttgc ttagtatact catcttctat aactgaactg
    16441 tcttgaacat catttaaatg aagacctagt actttacctt ttgcacttac tacataaaca
    16501 ttagaactta aaacgtcgcc caatgctccg gccagtaaat caaaagagac actgcttcca
    16561 ccacttgttt gtaatgtttt atttattttc cttgtttttt gtaacacttc acttgccatt
    16621 taatcatctc ctcatgaaat ttaggccttt taataacctt ttatattttc taatattatt
    16681 ttcacattta atgaaaattt atttttctat tagttgttta cacttgaaag attatcatag
    16741 attggaacct ttttcaagag aattatgtga tttttttcaa ttttcactct tttccgtcat
    16801 tatgctcttt tgtctcttta ttacattctt tattagaaca cactgttttt gtttcagatt
    16861 tagtcacttt ttcaaccata tatgaaccac acttctcaca aaactctcct gttggcttat
    16921 tccaagctac aaagtcacag tttggataat tagaacaccc ataaaaagct ttacctcttt
    16981 tagattttct aagtataata tctccttctt cacattttgg acatttaaca ccaattttat
    17041 tcataattgg tctagttgtt ttacaatcgg gatagttttt acaagccata aactttccaa
    17101 atctaccata tttgataacc atattagacc cacagttctc gcaaatctca tcagtttctt
    17161 catccatatt tactttttct atattttcaa gagcttcttc aatagcctct ttaagtggtg
    17221 catatgattc tgatacaatc tttttccagt ctgtattgcc ttcttctata acatcaagtt
    17281 tactttccat atcagcagta aaatctacat ctataaattt atggaaatta ttttccaaaa
    17341 tatcagtaac tattattcca agttctgttg gacatagact tgttccttgc ttttcaacat
    17401 actctctatt cagtatagta gctattgttg gagcatacgt acttggtcta cctataccta
    17461 gttcttctag tgtttttact aaactagctt ctgtatatct tgctggtggt tgtgtaaaat
    17521 gttggtctgg caatatattt tctatattta atatatctcc tttttctata cttggtaaaa
    17581 ttttgtcttc tctttctgaa aaattatata ccttggtata accatcaaat ttcatttttg
    17641 aaccagttgc cttaaataca atatcgccta ttttacattc tacatttagt atatcaaaca
    17701 ctgaatcttc catttgacta gctacaaatc ttctccaaat taaattgtat aacttgtact
    17761 ggtctttgct caatgaggct tttatactct caggagttct atcaactgag gttggtctta
    17821 ttgcctcatg ggcatcttga acctttttag tatccttttt ttctttttta tcaccagtgt
    17881 ttttatagta gttttctcct aaattgttta cgatatagcc cttagctttt tcttttgctt
    17941 catcagatat tcttttagag tcagttctta tatatgatat aagaccaaca gtaccttcac
    18001 cttctatgtc gataccttca tataattctt gtgctatcat cattgttttt ttagtagtaa
    18061 aaaataactt atttgctcct tcttgttgaa gcatacttgt tgtaaatggt tttggagctg
    18121 atttacgtct tgatttagat tctatcttct caacctttaa atctttacct tccatttctt
    18181 ttagtatttg atttacagct tcttcatttt ctaattcaat cttttcatca tttttaccat
    18241 aaaatttcaa agttatatct ttgtctttta cggtcttagc ctcaacatct attgtccagt
    18301 attcttttgg aataaatgcc tttatttctt tttctctgtc acaaattaac tttgttgtaa
    18361 cagattgcac tctacctgca ctaagacctt ttctaacctt ttgccataat ataggactta
    18421 tttgataacc taaaagtctg tctagcactc ttcttgcctg ttgtgcatca accaaattta
    18481 tatcaatatt tctaggatgt ttaattgctt ttttaattgc atccttagtt atctcattaa
    18541 actctattct acaatcctca gattcatcta aatttagtat atgagctaaa tgccatgata
    18601 tagcttcacc ttctctatca gggtcagttg ctagatatac ttgtttagat tttttagctt
    18661 ctttttttaa ttcttttatt acatcgcctt ttcctcttat atttatatat tgaggttcaa
    18721 aattattttc tatatctaca cctaatttac ttttaggtaa atctctgaca tgaccaactg
    18781 atgctttaac tgtgtaatga cttttcccta aaaatttttc tatagtttta gcttttgcag
    18841 gcgattctac gatgactaat gttttggcca taaaaccata cacccccact acctaatctt
    18901 aatttataca tttaaactgt acgttttatt attcatttct actactaact catttagtac
    18961 taattcattg attattgaat ttacatactt tatttctata ccagtataat cacaaatact
    19021 atctatatgc aagtttcctt ctctttttat agcttcaatt atttgcttag acttggcatt
    19081 tagacttata ttatcatatt tttgatttat ttttttatca ataatattaa atatgttata
    19141 ttcatttaaa atatcatcta tattttcaat taattttgca ccttctttta taattttatg
    19201 acaacctcta ctcatttgag aatttatatt accaggtatt gcaaaaacat tttttccatg
    19261 ttcaagagca aaatctactg tgattagtgc accacttttt attgctgctt ctacaacaac
    19321 tactccatca cttaaaccac tgataattct atttctatta gaaaaatggt acggggctac
    19381 tgtcgagtta atattatact ctgataataa taatccccca ttttctaata ttttatttga
    19441 taaatgtaaa ttttgttttg gaagaggatt gtctactcct gaaccaagta ctgctatagt
    19501 tttagatttt ccactcatac aacctatgtg cgagtatgca tctattccta tagctaatcc
    19561 acttactata ttaactccat attgggatag ttgacaactt attgtttttg cacaattaat
    19621 tccataggct gtaggttttc ttgaacctac tatagctata ttaaaattat tattaactaa
    19681 gcctatgtca cctttgtaga ataataactt tggagcgttg tatatattct ttaaattttc
    19741 aggatattct ttatcatatt tacaaatata tttaatcgct tttttgtaaa gtagttcttt
    19801 tatattttct aaataagcgt gacctctata ttttactata ttttttctaa tattcaagct
    19861 tatatttttt aaattatata tttctttttc agaaaaatca aatatatctt caatatttac
    19921 tatttcattt tctattattt caattgtttt tgttgttatt ccaccaattg attttaacca
    19981 taaataagta tctctttttt ccataaaatc cacctcaaaa aactatttaa aataactata
    20041 ataagccttt ctaaatgaaa aagcttctat aatacactgt gaatttatca agtctctttc
    20101 ttctaaatct gctattgttc ttgccatttt taaaagtttt gtataacttc tactgcttag
    20161 gttgtattta ttaaatatca tctcagccgt ttttgaagct tctttttcca gtttacaata
    20221 tttaaataaa ttgtaagctt taatttcact attatttttt atattatcct tcttaaatct
    20281 atttatctgt atttttctag cattttctac tcttctcttt attttttgag agctttcttc
    20341 ttgttttaaa gaattaaaat ctgaatattt tatagaatta acttctacaa aaatatcaaa
    20401 cctatctaac aatggacctg atattttatt tatgtatcta tcaatttcat tacttctaca
    20461 tctacactct gtgtctgaca tataataacc acatggacat ggattcatag ctgcaactaa
    20521 cagtactcta caaggatatt ttgcactgta ctttactctg gatatattta catatccatc
    20581 ttctataggt tgtcttaaag tctcaagtat ttttctatca aattcggcta tctcatctaa
    20641 aaataagata cctctatgtg ctaaagcaat ttctccaggt cttgcatcca ttcctcctcc
    20701 aattaaagat tgctttgtag ttgtatgatg aggtgctcta aaaggtcttt tatcaattat
    20761 tcctttagtt tcatttatca tacccaatat actatagacc ttacttactt ctatcatttc
    20821 ttctacactt atatctggta aaattgtcct tactcttttt gcaatcatag tttttcctga
    20881 gcctggaggt ccaatcataa acatattatg atttccagca gcagcaattt ctgcactcct
    20941 ctttacaaaa taatttcctt ttacatcttt aaagtcttca tcatatttac tttcttcatt
    21001 attaatctta gtatatttgc aatctatata actattatct ttttctaatt cttcgttctc
    21061 ttttcttgac ttatcatcca aaaaagacat tatccttact ttatcgactt ttatctcttc
    21121 gtttaaaaaa tcgacacatt cttttaaact tttaatagga attatatcaa tttcatctac
    21181 aaatagactt tcttttatgt tctcaattgg tataaaaata cgttttatgt tctgagtttt
    21241 tgcacccata attatgggca atatacctct tacttttctt attttcccat ctaatgataa
    21301 ttctcctata aacatacttt ctcttatgta attttcatct ttttttatta actctcttaa
    21361 aaggcctata cttatagata aatctaaaaa agctccttct ttttttatat ctgctggtga
    21421 taaatttaca actattctcg tgcttggaaa tttatatcca ctattaagaa ttgctgactt
    21481 tactctttct cttgcttctt taatttcttt gcctggaaga ccaacaatat taaaactagg
    21541 tattccattg cttacatcta cttcaacctt aactaaaaag ctgtcgattc ctactaagtt
    21601 gcttgaatta ataatactta acatatttaa tcctttccaa aaacattatc ttttttaaaa
    21661 agcattcatt atgtgattta ttcttttttc taataaatat acctctataa catcaaatct
    21721 tatgggagtg ttatttaagt tattttttat aatataataa cttgcaactc caattatctt
    21781 ttttcttttt ttgaagctta cagattcaca agggtagcca aactttatgt tagaccttga
    21841 tttcacctcc acaaatataa tttcatcttc catttttgct attatatcta tttcccctat
    21901 ctttaatctg taattttttt ctagtatctt tgctcctttt gatagcaaat aattaactgc
    21961 tactttttct ccaaaatcac ctttttcttt attattcatt attcactcca atacattaaa
    22021 ttttcacttt taaattatgg tcagtataca attttactat tcaagttttt ggattgattt
    22081 tatttccatc aatattaata aatacttcta ttgactttat tcttttatga tattattcta
    22141 gttttcatag gaaaagttta tttttttgga ggagtgcctt taatgaacag agaaaaatta
    22201 gttgctttta tacgatattt atctttattt atattagttg taagctattt gaaaaatgtt
    22261 gaaaatacat tgtatccggt cataactatg ataatattat ttctaattat aataaataat
    22321 caagttagat ttttttcttt atcaaatgag aatacattta tacttatttc ttacttttta
    22381 gaacttatat taattttttt actttgtaat tatacaaaaa cttttaattc gatttacttt
    22441 gttcctttaa tattagatat atcattttta ataaaggaaa aatataagta catattgttc
    22501 tcttttgtta taatatcttc tcttgttatt agtttagata aaaacatata tttggcactg
    22561 gagtcaagct ctatattgct aattataact gtactttcta tatatataga aaatgagaat
    22621 ttatcaaaat tatacagtca aaatctttat gataagctta gaatttctaa agatgaatta
    22681 aaaaaagtta atgctgattt ggaaacttat gcaagttcta tagaagaatt agcaatttta
    22741 aaagaaagaa atcgaatatc aagagaaata catgatagtg taggacattc tctttctaca
    22801 acaataatac aattaaatgc tatagaaaaa ctcttaaaag acaaacctct aatttatgac
    22861 ttagtacatg aacttagaga atttgtaact gaaagttttc aagatgtaag aagggctata
    22921 tctgagttaa aacctgttga atatgaaaat taccaaagtt tatttaaaat caaagaatta
    22981 gttaaaagct ttattaagct tacaaatatt aatgttaaat taactatttc aaaaaatact
    23041 tggaacttat ccagaaatca atctataact ttatatagac ttatacagga atccctttcc
    23101 aactcatcta ggcatggaaa ggcaactgaa atcagaattt ttattacatt caatacttca
    23161 aatcttataa ttactattag tgataatggt ataggatgtg gcaatataaa aaaaggaaat
    23221 ggacttaata gtatcaatga gagaatatat gaattaaatg gtaaagttga atttgataat
    23281 tctaataatg gttttacaat tagagcttct tttcctaaat tttcaggggg tgattttttt
    23341 gaataaaatt aaagttctta tagtagatga cgaaaaatta attagaaaag gtcttaaaat
    23401 tatattatca tcttataatg atttagaaat tgtaggagat gcttctaatg gatatgaagc
    23461 tttagaattt tgtaaaatga atgatgtaga catagttctt atggatatac gaatgaaagt
    23521 atgtgatggg gttctaggta caaggcttat aaaggaatat aataattcta taacattatt
    23581 aattttaaca acttttaatg atgatgaata tataaaagat gctatgaagt ttggtgcttc
    23641 aggatacctt ttaaaagata gctctgatga agtattgcat gaagggattc gttcttcatt
    23701 ttttggcaat attgtgcttg ataaaagtgt agctgaaaaa atcatgacct ctgaaaaaac
    23761 ggttaaacaa gagcatcttt atgacatgta caatttaaca gaaaaagaaa tatctataat
    23821 tagactaatt gcaaatggac ttaataataa agaaataagt caagaactat tcttatctga
    23881 aggtacaata aagaataaca taactaatat tttagctaaa ctagaacttc gtgacagaac
    23941 tcaactagca atctttgctt tcaaaaataa aattgtaata gaataaatct aaaaatataa
    24001 agtgacttaa gtcacttttt ttagtgactt atagtaatag aattataatt cacaattctt
    24061 tattataata ttatagcttt ttaacttata caaaattatt ttggagggat tactatggat
    24121 atagtcagag taaataatat aacaaaaaga tttaatgata aattagtact tgataacatc
    24181 agcttttctg ttaaaaaagg tgagatattt ggacttattg gcccaaatgg tgcaggtaaa
    24241 tctacactta taaatataat tacaaatctt atgttaccaa atagtggtag tattcaaatc
    24301 aatggtttag atttatcaaa agattatata aaagcaaagt ctataatagg gttagttcca
    24361 caagaattag caattataga aactcttact tcttttgata acttagagta ctttggagct
    24421 ttctacggtt taaagggaaa actacttaaa gaaagaatca ttgaagcatt agaagttaca
    24481 ggtcttactg aagtcaaaaa gaaaaaggtc aaaaaattat ctggtggtat gcaaaggaga
    24541 ttaaatattg gaatagcact cttaaatcat cctaaaatat taatattgga tgaaccaact
    24601 gtaggtgtag acccccaatc aagaaatcat attttcaatt ttataaaaga tatatctaaa
    24661 aaacatgaaa ctactgtaat atatacttcc cattatatgg aagaagttga gcacctttgt
    24721 tctaaaattt ttattatgga tgaaggtaaa gaaattgctt ttggaaataa tgattattta
    24781 aaatcactcg tatctacaaa tacaaaatta attatggaaa taaaaaatat aaatgcccaa
    24841 cttatctttg atttaaaaaa tactaaaggt gttatatctg ttttagaaaa taattcatta
    24901 ttagaattgg atatagataa aaaattacaa cttacagata tactctctgt cattgacaaa
    24961 aacaattcaa aaataatgaa aatttcttat gaagaaccta gcctagaaga cgtattttta
    25021 aatctaacag gaaaaaattt aagggactaa tggaggtgat tttatgaaat tatacatgag
    25081 tgtaaaaact atgctaaaga gtttaaaaag ctcctttata cttaatttaa tatatttttt
    25141 agctttgcct ctgattttat cttggtttct tgggatggtt acagaaagca tgtttcaaaa
    25201 ccctataaaa acagaatcta cccccattgt tatatatgac aaagataaca caagattatc
    25261 aaatgatttg acaaagtatt tgaaaaatga tttatcttat atattaacag ttaaaaaaga
    25321 tgattctaag gcagaattaa agcttactat acctaaaggc tatgagagta gtttattaaa
    25381 tgaaaaatca aatactctaa acatagaaaa acttggaact cgtgatgata tcgcaatatt
    25441 gcttcaagat atattagata cttaccatga aaaattttat ttaaataatt ctcagaaaat
    25501 aagttcagaa gatttttcta aattatttaa taaaaattct attgatactt ctataataaa
    25561 taacaatgtt aaacagagct cttatgaata ctttgcttta gtttcactag gatttcttgt
    25621 aataattttt ataatgaaca atattttgtc aaattatata agtgaatcaa agggactttc
    25681 aaaaagactt tattctatgc ctataactag agtacaattc ttaatttatg actttgtagg
    25741 tttatggatt tattcattta tattcttatt attatatgtt ttattcttta gaatacttgg
    25801 aataacattt aaaggtaatt ttgccatatt gctcctactt tgtgctttat catcttattt
    25861 tatgacttca atatctacct ttgtaaccag cttttttagc aaaaaatacg gtacagcaat
    25921 agtttatgca ttactctttt tacagacaat ttttggaggt atcttcagta tgataagtga
    25981 tacatttact aagcttacaa gtttatctcc tacctattta ataggagaat tgtttagtaa
    26041 ttatgaaaca tttaaaacta tagattcaat aggagattta attttcacat gtttaattat
    26101 ctctacaata ttaattgcac ttgctattgt aaaggaaaat tataaatgga gggaggttta
    26161 ataatgatta gattaactat tgcaaatata aaaagatatc taaaaaatca tactctttta
    26221 attaatatgg ttatgcttcc tattatacta attttttcac ttaatttttt tatcaacaac
    26281 tcgggaaatc aatcaatgta tttttctcca gtagcaattg tatctgactc ttcaggaaag
    26341 tatgaacata agttaataaa tagttctaaa ttaaaagaaa attcatttag attaaatgaa
    26401 caagataagg ctatgaatct attaaaaaat aataaggttt ctgctgtatt cgttttagat
    26461 aaaaattttt caaatagtat agataaatta caacgacctg tagttaaatg ctttaaaatt
    26521 gaaaatggtg gtggtagtct ttgggcagaa tcacaaatag aatcttttat tactaaatcg
    26581 ttaaaattga aagtggataa aaatatagat gataaattga caaagacaaa tataattgat
    26641 aacaaaccag ataagaataa aggttcattt ttagtagtct tcttgatatg ctattttatg
    26701 tatattaatg cggcacatct tgcttctgat ttatttacat taaaaaaatc aaatgtgcta
    26761 aaaagactta tctctacaga taataaagat ataaaaataa ttttcagtat atttttagga
    26821 ctatttttta ttcaatcaat tgtatacact tttgctctat tatgtttttc aattatagaa
    26881 gactttaatt tgagtctgaa tgtattgatg attgtactag caaatagttt tgtttccact
    26941 ggatttgtat tttgggtagc tagagtattt aaaaatgaaa gttctataag tcttatatct
    27001 acattttatt ctttaatagg tctagctata tctatctcta gtctaatacc atcaatggat
    27061 aaattatcct ttatgactaa tctttcaaag ttcacaccat tttattggac tatagatgct
    27121 attaaaaaca atggtagtat tttaataaat ataatagctc taatattaat tggaattata
    27181 tttgtaactg caggaagtct taaactaaga gattttgcta aaaactaata attattaaat
    27241 ataattagta tctattttta tataattgta aaagaatatt tcaacatatt aaattatttt
    27301 atcttatatt tttctcttta gtttcacaca tcaccaagtc taaactttat attatatatt
    27361 aatatttttg acttgtcaag ttatttatgt tataatatta atcattagat aattaaagca
    27421 ttgtacctaa gttgcaatat ctaaaattca atttggaggt ataactagca tatgtataaa
    27481 attggtgaaa gtgtaatgta tccaaaagag ggagcctgct ctgtaaatga tattgtaaca
    27541 aagaaaataa atcatgagat gcaaaaatac tatgaattaa gtgtcatttt taatagtaac
    27601 cttaaaattt cgattccagt tctaaatgca gatagaattg gcatacgtcc agttatggat
    27661 ggaaatgatg ttgataattt tatccaatct attaataaaa cagatggtgt atggattttt
    27721 gatagaaaag aaagacttaa actgtaccaa gataaatttc attcaggaga tgtttttgaa
    27781 attgtaaaat taatcaaaat gcttatgata caagacagtt caaaacaatt gtgtagcaca
    27841 gataaggagt ttttaaataa agctcaaaaa tttgctttat cagaattagc tgctgctcaa
    27901 tgtaaatcct atacaatggt attagaggaa atgaaaaaac atatattaaa ttcaaagaat
    27961 ataaattaaa ccagtttttt aaactggttt tattttttat actaatcatc catgctactt
    28021 tagcttattt taaactgcct aatctaatat taataaagtt atctcaatta gatagatgta
    28081 ttttattcaa ataaaaaaaa actaagagta aatcatatta ctgatttact cttagctaat
    28141 aaaatttaat cttcttcaag ttcttttttc tcttgtaata atacacttaa ttcatttgct
    28201 tctaaaactt gtgcctctgc aatatcaagt aactcttgtt gccattccac acatttatct
    28261 ttgtaatcaa aaggtagcgt tctgtcaact atgtacttat caatacagtc ctccgcactt
    28321 ttaattattt cttttctttc atcaataaat ttttctaatt ctactaaggc ttcttcataa
    28381 gcttttctac tcatataaat tccccctttg tacaattatt ctacattaaa atgatttttc
    28441 ctcttttttg tataaaattt tatttgtata taacttttcc atcaatatct tttatagttt
    28501 gacatccagt taatatcata gaactactta gctctgattg aagtttattt acataagttt
    28561 ctacaccatc agttgcacca ccaaaagaag cagtcacaaa aggtcttcct attaatactg
    28621 catctgcacc taaacctatc atttttagta cgtctaaacc agttctcact ccaccatctg
    28681 caagtattgt aactttacct ttaacagcat cagcaatttt tggtaaaact tcacatgctc
    28741 ctggtgtaca gtcaagcact cttcctccat gatttgatac gactattgca tccacacctg
    28801 cttctactgc cattaaagca tcttcaacag tcattatacc tttcaatata aatggtagtt
    28861 tagtagactt tactaactcc tttatttgtt taacattttt aggaagtact ggttttccat
    28921 gtaatgataa agttactaga ccacaggcat ctatatcaac tccaactgca aatgcaccag
    28981 cttcttcagc taatcttatc ttttttataa tattttcatt atcccaaggt ttaataaata
    29041 caattccagc tccattatac tttttgacta catctaaatt ttccattaaa aaagcatcta
    29101 ctgccgtatc accaaccatt gcataaatac cactattagc acatcctgca actactggtt
    29161 ctatgtaatc tctttcatta atttttccac ccatatttaa agtagttcct gttactggag
    29221 ctgcaaatat aggtgcactc attttctttc caaacatctc tatagatgta tcaggatttg
    29281 atacatcatg tattactctc atatttactt ttactttttc taaactcttg ctattttcta
    29341 taaaagaaga accacttcct ttacctccca tgccaggcac ttcacctgca caagcaaccc
    29401 cattacaaac cttacaaact ttacaacttc catttaaatt ttctctagca ctttttaata
    29461 attcattata ttccatcttt aatcccccat taactatagt aatttatatt atatacatta
    29521 tatcatatta tatttacagt atatttttca aaaaactttt tctgtggata ggagttatgc
    29581 catatttttc aatagcttca tagtgttctt tagttccata tcctttgtga gatttaaacc
    29641 catattctgg gtatactcta tcatattggt acattatact atccctagta actttagcaa
    29701 gtatacttgc tgctgctatt gatatagatt ttgaatctcc tttcacaatt ggattttgag
    29761 atatgtcaat acctggtatt gttgctgcat caactaataa ataatccggt gcttttttta
    29821 aacaatttat agcctttttc attgccatat atgtagcatt taatatatta aattcatcta
    29881 tttcttcatt gtttactata cctattccat aatctagtgc ttcttctttt attacttcaa
    29941 aaagttcatc tctttttgcc tcactcagtt tcttagagtc atttacacct tctattttgg
    30001 tatctttttt aaatactaca acagaagcta caacaggtcc agcaagtggt cctcttcccg
    30061 cctcatcaat tcctccaata tataaataac ctttatcata gccttcgttc tcaaatatat
    30121 ttatagtttc caatctttct tcttcttttc taatattatc cagctttttt gctagtttta
    30181 tagctagacc ttgtacagat tttctttcat caactcttaa cagctctata tattccatat
    30241 atttttctac ttctaaagtc tctattattt ctttaatctc cctcacactt ttatcctgca
    30301 taccattccc tcatctttcg tttttaaaaa tttaattttt aaatatatta aaatttttta
    30361 attagagttt aattgcatcc actatagcca cattttaaac atatactaca tccacctgta
    30421 tgtgcaattc cttcacttcc acactcaggg cacacaaatt tttcatcttc ttcaaaattt
    30481 ttatttatta tttctttggc tttttcatct ttattatctt tttctttttt attattttta
    30541 taatattctt ttttactatt ttctttttta tcttctttat tattttcttc ctttttttca
    30601 tttattaaaa ttccactcct tttacatcca tctctaaata ttgttactcc tttaagacca
    30661 gcctcccatg ccttaatata tatattgaaa acatcttcaa ctgtagtttc aaaaggtata
    30721 tttattgtag atgatataga agcatcaata tacttttgcc atactttttg catgtttatt
    30781 ctttcttcat atcctatatt catagcagta acaaaaaaat ctggtaaaca ttcttcatct
    30841 gtaatacctt tcttgtccat atactcttta acaattggag tatatacttt ataatacaca
    30901 tcctcatcat gcaagctttc agtttttctt atatatgaaa gattaaatat aggttctatt
    30961 ccaccactga tacctatcat tgtagatatg gaacctgtag gaggtatggt taaaagttgt
    31021 gaatttctaa gcccatactt tttaaccatt tccttagtgt cttcatttag attttctata
    31081 aaaaacttag atttagaaat attttcatat ttatactcac taaatgttcc atattcttta
    31141 gcaattaatg ctgattgctt cacagcttca tttaacatag tcttaccaat taaatcacat
    31201 agttctaaag attcatcaga accatatctt attcccattt ttataagcat atcagctata
    31261 cccattacac ctaaacctat ctgtctatac ttatgtacac tatctctttg ttcttgtaat
    31321 ggatgcaagt tcaatccctc atctaagaca tcatttaaag ctactacagc ttctctaacg
    31381 caagatttta atttattaat atcaaaagta gcatgttcac caaatggttg tattataaac
    31441 tctgacaagt ttattgagcc aagtagacaa cttcctccac ttggaagtgg ttcttctgca
    31501 caaggattta ctcctgaaaa gcaaaagttc ttatcttcac tcataaaatt atatgttttt
    31561 attttatccc aaaacaatat acctggttct gcataatccc agttattttt cacaaattta
    31621 ttaaataaat cataagcatt tactatctta actatttttt ctccagtagt ttcaacttca
    31681 aaagtacaat tatataactt tttttctctt acagcttgca taaattcatc tgttactctt
    31741 acagaaatat ttgctttagt aaccttctcc aaatctgttt ttatatctat aaagtcctct
    31801 atctctggat gatttacatc catagaaatc ataagagctc ctcttctccc tctttgacca
    31861 attaagccag ttgtcatact gtaaagttcc ataaaagaga ctgctccagt cgtatgtttt
    31921 gcagcattgt taactttcgc tccttttggt cttagctttg aaatatcaat tccaactcct
    31981 ccaccataac taaaagttct agcaagtttt tttgcagtat caaatatatc ttctatgcta
    32041 tcttttggtg gttctattac ataacaatta gaatatgtta ctttttttcc ttctttctgt
    32101 aatcctctat ttgctaaaat tcttccagca aatagaaatc ttttttgtct tattagtttt
    32161 gatattcttg aactattatt tgaaattctc tttatccatt catcaaaact ctcaccatta
    32221 tgtctatact tgttcttcca tattgatttt tgtaattcat caaactccca agctttagtc
    32281 cttatttcag agcgtctttc tctatataat atgtagtgtt ttgctatatc ttttctattt
    32341 gattccataa gtagtacttc aactatatct tgtatttctt ctacggataa gcatttttcc
    32401 atagaattat agtcattagc tatttttctt gatatatcat atgccaactc tctatctact
    32461 ccaccttcac tatttatact agctttatag acagcatctt caatcttact tttactaaaa
    32521 agttcttttg taccatctct ttttattatg tacttcattt ttaaacctcc aataaattag
    32581 tattaaatta atacacacat taactataac tctaaacaaa ctttttatat atttgtatat
    32641 tttcat
//
LOCUS       DAELNY010000024        32544 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.024, whole genome shotgun sequence.
ACCESSION   DAELNY010000024 DAELNY010000000
VERSION     DAELNY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.024"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            151..1320
                     /locus_tag="KPE55_000994"
     CDS             151..1320
                     /locus_tag="KPE55_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0492090.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            1369..2220
                     /locus_tag="KPE55_000995"
     CDS             1369..2220
                     /locus_tag="KPE55_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0492091.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            2546..4447
                     /locus_tag="KPE55_000996"
     CDS             2546..4447
                     /locus_tag="KPE55_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0492092.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            4450..5493
                     /gene="ypdE"
                     /locus_tag="KPE55_000997"
     CDS             4450..5493
                     /gene="ypdE"
                     /locus_tag="KPE55_000997"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0492093.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            5508..5813
                     /locus_tag="KPE55_000998"
     CDS             5508..5813
                     /locus_tag="KPE55_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0492094.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            5910..6245
                     /locus_tag="KPE55_000999"
     CDS             5910..6245
                     /locus_tag="KPE55_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0492095.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            6277..7599
                     /locus_tag="KPE55_001000"
     CDS             6277..7599
                     /locus_tag="KPE55_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0492096.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            7609..8679
                     /locus_tag="KPE55_001001"
     CDS             7609..8679
                     /locus_tag="KPE55_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0492097.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            8684..9871
                     /locus_tag="KPE55_001002"
     CDS             8684..9871
                     /locus_tag="KPE55_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0492098.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(10068..10940)
                     /locus_tag="KPE55_001003"
     CDS             complement(10068..10940)
                     /locus_tag="KPE55_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG0492099.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(11334..11525)
                     /locus_tag="KPE55_001004"
     CDS             complement(11334..11525)
                     /locus_tag="KPE55_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492100.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            11745..12791
                     /gene="cobT"
                     /locus_tag="KPE55_001005"
     CDS             11745..12791
                     /gene="cobT"
                     /locus_tag="KPE55_001005"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG0492101.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            12823..13383
                     /gene="cobU"
                     /locus_tag="KPE55_001006"
     CDS             12823..13383
                     /gene="cobU"
                     /locus_tag="KPE55_001006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG0492102.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            13393..14166
                     /gene="cobS"
                     /locus_tag="KPE55_001007"
     CDS             13393..14166
                     /gene="cobS"
                     /locus_tag="KPE55_001007"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG0492103.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            14186..14800
                     /locus_tag="KPE55_001008"
     CDS             14186..14800
                     /locus_tag="KPE55_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0492104.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      14867..15046
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15176..16675
                     /locus_tag="KPE55_001009"
     CDS             15176..16675
                     /locus_tag="KPE55_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG0492105.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            16714..18090
                     /locus_tag="KPE55_001010"
     CDS             16714..18090
                     /locus_tag="KPE55_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG0492106.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            18091..19062
                     /locus_tag="KPE55_001011"
     CDS             18091..19062
                     /locus_tag="KPE55_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0492107.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQCIIRKVS
                     KNDRQLKFGGFILWLITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            19094..20164
                     /locus_tag="KPE55_001012"
     CDS             19094..20164
                     /locus_tag="KPE55_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0492108.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            20166..21053
                     /locus_tag="KPE55_001013"
     CDS             20166..21053
                     /locus_tag="KPE55_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0492109.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            21041..21673
                     /locus_tag="KPE55_001014"
     CDS             21041..21673
                     /locus_tag="KPE55_001014"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG0492110.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            21682..22908
                     /locus_tag="KPE55_001015"
     CDS             21682..22908
                     /locus_tag="KPE55_001015"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG0492111.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            22905..23513
                     /locus_tag="KPE55_001016"
     CDS             22905..23513
                     /locus_tag="KPE55_001016"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG0492112.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            23503..24078
                     /locus_tag="KPE55_001017"
     CDS             23503..24078
                     /locus_tag="KPE55_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG0492113.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGCAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            24071..24823
                     /locus_tag="KPE55_001018"
     CDS             24071..24823
                     /locus_tag="KPE55_001018"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG0492114.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            24807..25928
                     /gene="cbiG"
                     /locus_tag="KPE55_001019"
     CDS             24807..25928
                     /gene="cbiG"
                     /locus_tag="KPE55_001019"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG0492115.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            25925..26650
                     /gene="cobJ"
                     /locus_tag="KPE55_001020"
     CDS             25925..26650
                     /gene="cobJ"
                     /locus_tag="KPE55_001020"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG0492116.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            26647..27399
                     /gene="cobK"
                     /locus_tag="KPE55_001021"
     CDS             26647..27399
                     /gene="cobK"
                     /locus_tag="KPE55_001021"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG0492117.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            27418..28896
                     /locus_tag="KPE55_001022"
     CDS             27418..28896
                     /locus_tag="KPE55_001022"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG0492118.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            28926..29831
                     /gene="hemC"
                     /locus_tag="KPE55_001023"
     CDS             28926..29831
                     /gene="hemC"
                     /locus_tag="KPE55_001023"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG0492119.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPIAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            29821..31320
                     /gene="cobA"
                     /locus_tag="KPE55_001024"
     CDS             29821..31320
                     /gene="cobA"
                     /locus_tag="KPE55_001024"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG0492120.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            31337..32302
                     /gene="hemB"
                     /locus_tag="KPE55_001025"
     CDS             31337..32302
                     /gene="hemB"
                     /locus_tag="KPE55_001025"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG0492121.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            32455..>32544
                     /locus_tag="KPE55_001026"
     CDS             32455..>32544
                     /locus_tag="KPE55_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0492122.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNF"
BASE COUNT        13144 a         3272 c         6014 g        10114 t
ORIGIN      
        1 attaattaaa atctatagtg tatattttat agtgagttta agtagtacta aaaaatctaa
       61 gcaaaaaatt ataaaagagt atattttttt atagtttaaa gtggttataa cctcattacc
      121 aaatgtaaac gttacaagta tgaaaggaga atgaaaatgt ttaaattaga agaacaaaaa
      181 ctaaaagatt taggtgcaat aataacaact aatgaaataa aacaacagcc tgaattatgg
      241 ttagaaactt atgaaatata taaatctaac aaagaaaaat taagtagatt catagataca
      301 ataagtaata atcatgggca gtttagagtt atatttacag gtgctggaac ttcagcttac
      361 ataggaaatt caatacttcc ttaccttaaa aataaaaatg acataagaaa atacatattt
      421 gaagcaatac ctactacgga tatagtttca aacccatatg attacttaaa aaaagatata
      481 ccaacattat taatttcatt tgcaagaagt ggaaatagtc ctgaaagttt agcagcttta
      541 aatttaggga ataagattgt agataatttt tatcatttag ctataacttg taatccagaa
      601 ggagaactgg ctaagatgac taaaaacgac gaaaataatt acttgttatt aatgccatca
      661 aaatcaaatg atgaaggctt tgccatgaca ggaagttttt cgtgtatgat gttatctgct
      721 atgttaatat tcgatagttt agaagatgat gtagaaaagt catatataaa tgcaataatt
      781 gaaatgggaa gaaatgttat agatagaaaa gatgaaatac atgagttaat aaataaagat
      841 tttgatagag ttgtatattt aggttcagga ggattaggtg gtttgacaca agaagcacaa
      901 ctaaaacttt tagaacttac agcaggaaaa atatctacag tgtatgattc tcctatgggg
      961 tttagacacg gaccaaaatc atttattgat gaaaatacac ttgtatttga atttgtatct
     1021 aactgtttat atacaagaaa atatgattta gatgttttag aggagataaa aagggataaa
     1081 attgctaaat tcacttgtgc agtgtcagta gaaaatgaaa ataacttctc aggaacaaag
     1141 tttgagttta aagaaaagta taataaatta ccagatgtgt atcttgcaat gccatacata
     1201 ttgtttgcac aaactatagc attgtttgta tcagtaaagg ttggaaataa gccagatact
     1261 ccatcagcta caggaacagt aaatcgagta gtaaaaggtg taacaatata tgaatattaa
     1321 aagataagat taaaatatta taactaaata atttgggagg gtatcaatgt ggcattagta
     1381 acaacaacat caatgcttaa aaaggcacaa gatgaagggt atgcagtagg agcatttaat
     1441 gtagaaaata tggaaatggt aattgctgtt atagaagctg ctgaggagtt aaattctcca
     1501 gtaatattac aaactactcc atctactgta aaatatgcag gtcttgatta ttatctagca
     1561 aatgcaaagg ttgctgcaga aagagtaaat atacctgtag caatgcatct ggaccatggt
     1621 tcaagttttg accttgcaat gaaagcttta agaagtggat atacatcaat aatgatagat
     1681 ggttctcatg aaagctttga aaataatata tctatatcta aatcagtagt aaaagcatgt
     1741 agtccttctc ttataccagt ggaagcagaa cttgggaaag ttggtggaaa agaagatgat
     1801 ttagatggcg ggaatagtaa tgattataca aatccaaaag aggcaaaaga gtttgtcgat
     1861 agaacaggaa tatcttcatt ggctattgct ataggtactg cacatggatt gtataagggt
     1921 gaacctaaaa tagatttaga taggttatca gaaattaaaa atgtagtttc aataccttta
     1981 gtattgcatg gaggttcagg aatacatgat gatattataa aagaagctat aaaaagaggt
     2041 attgcaaaag ttaactatgc aactgaatta agaattgcat atagtaaagg ggttaaaaaa
     2101 gttttagatg aagattctga ggttatagac cctaaaaagt atggattagc agggcttcaa
     2161 tcagtaaagg attttgtgaa agaaaagatt aaagtgtgtg ggtctgtaaa tagagtataa
     2221 aaataaaata tcctaaattt attttagata agtttaggat atttattata tgaaaaattt
     2281 atattaatta tatgtaaatg gtgtattttt ttcatttaat tgatgaaaaa tatcagcttt
     2341 tattggtaaa gtattacata tataattaaa atatactaaa aattgaacta tatattttat
     2401 tattataaaa tatttatatt aaattgtgga attaaaatat actaggatat aaaattctta
     2461 aataaaaaga aaaggttgtt tttataaaaa atatttttta taccacttta aaaaacacaa
     2521 taataacact gagggaggtg gggatatgag agaacgcatt attcttgaaa cattaaaaaa
     2581 tagtagtggt tatctaaaca tagattattt tgcagataag ttgggggtat ctactcgaac
     2641 tatcagaaat gaaataaaaa agatagaaac tataaaagaa tgtaatggtt ttaaattaga
     2701 atataagtct aaattaggat atattctaaa tattaaagac caagataaat tcgagcatta
     2761 tctaagaaac ttaccctcag atttagttga aaatccagaa caacggttgg agtctataat
     2821 agtagagctt ttagtaaatg aaggttataa aactatagaa cagttaagta aaaaatttct
     2881 agtaagttca tcccaaataa aaaatgattt aaaaaagata gatgaaaaga taaaggatac
     2941 agaattaaaa ctagagcgaa aggctcatta tggaattaaa atagaaggtt ctataaaaga
     3001 aattcaaagt atattggtgg atagttattt tagagggaat agaaatataa cagagtacag
     3061 gaataagttt atagataatt taaaattagc taatattaga agtacaataa aaaatgtact
     3121 taatgaacat gatttagaag caaatttaac agaacttgag gaaatattag cacaaattat
     3181 tatattgtat ataagagtta gtatgagagt agtgggaaat gctagcgatt taaaattata
     3241 tacagaagat ttaattatag ataaattatt agataaaatt tttagagata aaaaatataa
     3301 ccttaattat gatgaaaagt attatttaaa ggagcttata aaactaaaga ctaaagataa
     3361 gaaagccact ataaagaata ttgataagaa taaacttcaa gatattatgt tcgaattttt
     3421 tagagagata gataaaaaat ataactctaa ttttttagaa gataaagagt tttttaactt
     3481 attttactta catattgcat gtttaataga acgtataaag aagaaccaca agataataaa
     3541 tcctttttca gttaaaataa gtcaacagta tccaactgtt tttaatttat ctattcaatt
     3601 ttcaaaaatc atagaaaatg agtatcatat aaaaataagt caagatgaaa tagggtttat
     3661 agctactcac attgctgttc cttttgaaaa gagagaagaa gctaatttta ataaaaaata
     3721 taaaatagca ataatatgct cttctggtgg aggtagtgca tttttgataa atttaagact
     3781 taaagaaatt tttccaaatg ctgagataag aaatttttca ttactagaag aaaaagctgt
     3841 aatagaattt gcaccagatt tgattttttc aataacaaat cttttatttg aaataaatgc
     3901 tccagttata cttataaatg aaattttaga cgaattggat tatttagata ttaaagaaag
     3961 tgttagattt gcagaccata taggaaatat atcaagccca aaacaatata tattaggtct
     4021 atttgataag aatcacttta ggtgtataaa agataaagct aagtataagg atatccttga
     4081 tagtatgtca aaatgtatag ttgacgaggg agcttgttct ccaacctatc ccaaagatgt
     4141 atgggaaaga gaaagttatt tgagtactat ttatacaaat ggggtggcta tacctcatcc
     4201 aatcgagatg acaggaaaca aaaatataat atcagtagct ttgatacaaa ctgatattat
     4261 ttatgaaaat agggtgccta aaattatttt catgatttca ttaataaagg gaaatttaga
     4321 acttcataag caaatatcta aatatttaag taagattatg gcaaataagg atatggtaga
     4381 catgctaaat aagtcacaat cttatgaaga gtttatgtat aagttaaaaa tctatctggg
     4441 aggttagaca tgaatataga gttttttaag aggttgtcag aagcagatgg tatagcatct
     4501 aatgaagaag aggttagaca tgttttattg gaagaactaa aagcttatag tgataaaatt
     4561 atctgtgatg ggctagggag tatcatcttt tctaaaataa aagatgagag tgcaccaaac
     4621 gtaatgattt gtgctcatat ggatgaagtt ggatttatgg tgagaagtat agacaaactt
     4681 ggtatgattc acttaataac aataggagga gtaaaacctc ttgcacagtt tgtacaaaaa
     4741 gttagaatta caacaaaaga agggaagaaa atccccgctg tcataaatgc tacatataat
     4801 aatggtaagg ctgaaaatat atatgcagat attggagcat atacagaaga agatgtatat
     4861 aacctaggga ttaatgttgg ggatatggta acatatacaa caagctttga ggaatttact
     4921 ttaccagacc gattagtggg aaaggctttt gatgatagga taggatgttt tgtgatggga
     4981 gaagttttaa aagaacttag gaaagaaaat ctaaattgta atattcactt tgcggctaca
     5041 agtagtgaag aggttggaat aaggggagca aagacatcaa cacagttaat aaatccagat
     5101 atagtatttg taatagatgt agcatgtgct aaaaatgagt ttgtgagaga ctatacaaat
     5161 aagaaacaaa ttgataaagg tgtaatgcta atgcatagag ataggactct agtccctagt
     5221 agaaaaatga tagattatgt aatggaaatt gctgataaaa ataatatacc acttcaacat
     5281 gatatgtttg aatcaggtgg gactgatgga ggagaagcac atctcgtaaa tgaagggaaa
     5341 ccttgtgtag taacttgtgt accagttaga tatggtcatt gtgcattttc aatagtaagt
     5401 aataaagatt tggagaacat tataagattg tatacacagt taattttaaa ttttgatgaa
     5461 gaacaatata aacatataag aaattttatc taaaggagaa taagaaaatg tcaacagaag
     5521 tacaaataat ggaacttata tcaactgcag gagagagtaa atcgaaagct tttgaggctt
     5581 taaaaaaagt gaagtcaaaa gattttaaag gagcaaaatt gttaatagag gaggctcgta
     5641 aaatagatat tgaagctcac aggatacaaa cagaattaat aactcaagat ttaaatgaag
     5701 atgttgaaag tccagcaatg agtcttttaa tggtacatgc tcaagaccac tatatgactt
     5761 ctcagctagc aaaagacttg atagaggctt tgatatattg tcttgataag taattgtaat
     5821 atagaaatag attatttatg taaaaattct tttaaaagtt tagatgcttt taagagataa
     5881 taaaaataca aactttagga ggaattatta tgaaaatatt attatgttgt gcaggtggat
     5941 tttctactaa tatgttaatg caaaatatga aaaaggtggt aaaagagagt gctaaactca
     6001 atgaaaaaga ttttgagttt acagctatac cagcagattc aatagaaagt gaagttgatg
     6061 attgggatat agtacttata ggtcctcaaa tagctcacaa aatagacttc ttaaaaggaa
     6121 tattagagcc aaaaaataag ccttttgcag taatagataa agatgtatat ggaaaaatgg
     6181 atggagctac agttatgaag ttggcattaa taacttataa gaaacatcaa ttagctcaag
     6241 gataataaaa ataatgaggg aaaagggaga ttagatatgt tttcaaaatt tgaagcattt
     6301 atgaatagag tatttatgcc tttagctcat aaggttgaca atcaacgtca ccttggttct
     6361 ataaaggctg gtatggttgc tatgacacca ttcacaatat taggaagtat atttgcaata
     6421 cttccagcac ttccaaatat gcttggagaa aataatccaa taagcaaatt catacttagc
     6481 aattcagcaa tattggattt accagtaagt ctttccatag gtttaatagg attatatgct
     6541 tgtatgtgta ttgcttttaa tctggggaac tactataaat tatatatacc aggttgtgtt
     6601 gcactatcaa ctttttcttt tttattccta gtagcctcat ttacaccaga tggagcttta
     6661 gaaataggaa atttaggagc taaagggcta tttacaggta tgatagcagg gcttggttct
     6721 gtggagttat ataatttttg taataagaag aatatcacaa taaaaatgcc agaaaatgta
     6781 cctgattttg tatctaggtc ttttgaatta atacctacta ctataatagt ttgtggaact
     6841 tttatagttg ctcgttttat ttcattaaaa gtatttggag aattacctcc acagatactt
     6901 actcaatttt tatctccatt agtaggaagt atggataacc catggtcagt tcttgcactt
     6961 aactttgcta tatgtactat attctttttt ggaatacatt catcagtatt tagtcctata
     7021 actagaccta taatgattac atttatagct gaaaacatag cagctatgca ggctggagaa
     7081 acaatacctc atttctttac agcaggagct tcaagtgcat tctttggatt tacaggatgt
     7141 ggaattagta taggttgtgt aattgcatgt atgttatcaa aaaatgcttc ctataagaag
     7201 attggacgtg tatcattatt ccctgcatta tttgggataa atgaaccaat attatttgga
     7261 gcaccgataa tattaaatcc aataatgttt ataccacatg tatttggtgg agctataata
     7321 ggtacactac caatgttttt tatgcactgg ggatggttag caaagccagt atttgaccct
     7381 ccatatgtag gggtattctt agaaggattt ttaactaatg gagattggca ttctatatta
     7441 gcaaatgcat tgcaattagt attagcagtt ttattatatt ggccattctt taaaataatg
     7501 gaacgtcaac aaaataataa aaaacaaaaa gaagaaacta agaatatatt ctctaaagaa
     7561 gaagaagact tattggatga tttaggattg gatttctaat attttgttat gaataataca
     7621 atggagagat tagtatcctt aatctataaa agaggtgttg acgctctttt gattaaagca
     7681 aaaaatacta aaagatatat aggagcttta tcaggtagtg gtgtatatgt gcttgttact
     7741 aaggataaaa attatcaaat tctagatggc agatatattg atgaagcaga taaaaaaacg
     7801 actggtttca taaaaaggat tgtatctcaa ggttcttata taccaactat aattgagtta
     7861 ttaaaagaat taaatataaa aaagttagca atagaaaacc aagctatgag tatacaagag
     7921 tatctttcac taaaaaatga aggttttgaa atcaatttaa taacaaatga attatggaag
     7981 ctaagggcta taaaatctaa agaagaaatt aaacttatta aaaaggcttg tgagattaca
     8041 gatgaagtat ttgatgaggt tatttctgaa attaaaaaag atatgactga gctagaagta
     8101 tcagctctaa ttcagtatca tgcattaaaa aaaggtgcaa gtggaatgtc ttttgaaaca
     8161 atagtggtat caggagaaag aggtgcaatg ccacatggaa gacctacaaa caaaaaattg
     8221 aaagtgaatg aagctataac aatagatttt ggtgttgttt atcaagggta tcaatcagac
     8281 atgactcgta caatatctat aggcaaacct cctaaaatta ttaaagagat atatgatgta
     8341 gtattggaag cacaattatc tgcaatagag tcaataaagg aaggaactag ggcaagtgat
     8401 gttgacaaag tggctagaaa aattatagat aaacatggat ttggcgaata ttttaatcat
     8461 ggactgggtc atggtatagg tcttggagat ggtgaagttc ccactttaaa tccaaatagt
     8521 gaagatatat tagtggaagg aatggtaatg tcttgtgagc caggtattta tattccaaat
     8581 gtagggggag ttagaataga agatgatatt gttataatag atggaaaagg tgttccactc
     8641 aataagacat ccaaagagtt tataatatta ggagagtaaa attatgaaat ataattttaa
     8701 taaagtatta aatcgtaaag aaggaaattg tagaaaatgg agcaaccatg tgattaaaga
     8761 aaaatttggc ttaaatgaag atgcaattcc tatggattta gctgatatcg attttgagtg
     8821 tgctcctgct ataaaagaat ctatcttaaa aagagcatca gtaggagatt atagttatac
     8881 ttttatagga gatgacttct atgatgctat tataaattgg aataaaagaa gatttgatgt
     8941 atatatagaa aaggattgga taaaattaac ttttggtact gtttcaaccc ttcattatat
     9001 agtccaagca tatactaaag aaggagaggg tgtattgata aatacacctg cttatgaccc
     9061 ttttgcagag gctgtagtaa ataataatag aaagttgtgt tgtagctcat taaaattaga
     9121 taacaataga tattatttag atttagagga tatggaaaat caaatgaaat acgagaatat
     9181 aagattgctt atattctgta gtccacaaaa tccatcaggt cgtatatgga caaaagaaga
     9241 actatatcaa gtgtcagagt tatgtttaaa gtataatgta attttggtaa gtgatgaaat
     9301 tcatagagat ataatattta aagattatag atttgtatct ttgtggaatg ctcatccaga
     9361 aatttataaa aatagtataa tctgtgtatc tcctaataaa ggcttcaact taggtggatt
     9421 aaaaacatca tatgtattaa taagagatgt aaaaattagg gagactttat tagaaaggct
     9481 aaaaagtaat tcaataactt ctccaaatgt atttgcaata ccagcaatag ttgcagcata
     9541 taatgaaagc gaagaatggc tagatgctat gactagttat gtagaggaga attttgaaat
     9601 agtttatgat ttctttgaaa ctaatatacc aaaagctaaa gtgatgaagt cagactcttc
     9661 ttttttagcc tggatagatg taagagaagt atttagaaat gaagaagaat ctaaagaatt
     9721 ttttagacat gcaaacttaa ctatggtagt tggaagttat tttgtgaaag atggagatgg
     9781 atttattcgt attaatgtgg ggtgtcctag agaaacatta aatgaggctc taaatagaat
     9841 caaaaaaacg tatataagta tgtattgtta aatcataaaa aaagtatcta tggtttatat
     9901 aaaaacacat tatttagtta tcaaatatta ctggaatttt gtattccagt aatatttttt
     9961 gattaaggaa tattattgag gaatattaaa taatattatt tcataccctt atattaactt
    10021 atataaaaat attttattat tttgggaacg ggttagctaa cctttatcta gtgtatattt
    10081 atattgtaag aagcagtaaa aacttttcct ttactaagtt tattaataaa cttcttatct
    10141 ttataaattt tgttagaatt tatactatca gcaagaccat accaaggttc tatacataaa
    10201 aaaggagcag tagaattagt ttcactataa taaggagtcc aaataccaac tagagggaaa
    10261 tcttccatag atatagttac atatttacta ccatcattat tacatataga cacttcatct
    10321 atgttggtgt atatcaaagc atcattcttg aatacttctg gatttaactg taagtttttt
    10381 aattttccaa taggtattat atcatctgta aaagaaccat ttaaattgat tttttctaca
    10441 tcatttttac ttttaaactc aaggtagtgt ttagaaaaat cattttgttc gtaaaaagga
    10501 aggttgaatg caggatgtcc acctatagaa aagaatatat ctttagaatc agtatttttt
    10561 acaatccatt ctacctcaat actattatca tttaatgtat agtttatgat tagctcaaaa
    10621 gaatatggat acttctttaa agtagattca ttatctgtta atttataagt tacaaatgta
    10681 tctcctttat ctacaatctc aaagtccata tctctagcaa acccatgttg agtcatgcta
    10741 tataaatttt cttctattat tgtttcatta tcaaaaagtt tacctactat agggaacaat
    10801 ataggtgatt gtctacccca atatttttta ttaccatccc aaagaatgtt tttgtcgtat
    10861 tttttagaaa atattttagt tagttcagca ccaaaactgc ttgattcgat aattaaattt
    10921 tcattttgta atatattcat ttaaaatctc ctttattata ttttatgagt atattatact
    10981 ataaagtata aagattatta aattttattt gatattatat ttgaaaggta gattttagta
    11041 tattttgaaa attttgtttt gaaaatttgg gattatttaa gaaattttgt attataaata
    11101 gaaggaaata gataaattgt atagaataaa atatattaag ataagttaat aatgttaaat
    11161 gggaagtgag tttatggaaa aaccaaatac aaaagttgtt aactttcaag agtataaaga
    11221 cagaaaaaac caaagagaag taaatagaga cgaactttta aagcttataa aaaaaattgt
    11281 gacttcaaaa tagggagttg ttaaactccc tattttaatg ggtttcataa taattaagac
    11341 attctaagtc catctatata atctttgaat tgtgagaaat ctgattcact acttaattct
    11401 atggtttctg aaccttccgt ataaaaattc ttaagataga tagtataatt attatcaaca
    11461 caatcatgat aaatacatct atatccatct tcatacaaac tttttaactt agaaaaatca
    11521 tccacaatat catctcctta aagttctttt aaatatattt ttacaaaaag catctaattg
    11581 tatgcaaatt gacaatgtaa aataaaggaa atgaacataa aatgtagaat aatttttata
    11641 tgtatgtaaa aataaaaaga aaaagttgaa atttttagaa aataagagta ttttttatct
    11701 aaaaagagag tttaaaaaat aaaaaaatgt tgggaggata aaaaatgagt ttattagaaa
    11761 gtatatcaaa aaacatatat tccctagata actcttctat tgaaaaaaca aagcagagat
    11821 tggataggct tatacatcca actggaagct taggaaagat agaagatatt tgcatgcaat
    11881 tagcaggaat atttggcaat gaaaattttg acacaagtaa aaaggttata attgcttttg
    11941 cgggagacca tggtgtatat gaagaaggcg tagcacctga ccctcaaaat ataacaaaat
    12001 tacagtttcc aaatttttcg aaaggtctat gcggagttgg agtaataagt aaatttgtag
    12061 gggcagatgt tgtagctgta gatgtaggaa taaactgtga tgaaaaactg gatggggtat
    12121 tagactacaa gataagaaaa gggacatcaa atatggcaaa aggtccagct atgagtaaac
    12181 aagaagcaat tagatgttta gagataggta ttgaaatagc agagcagtgt atagaaaaag
    12241 attataaggt tattggtatt ggggagatgg gtatagcaaa tactactcca agtacagcaa
    12301 taatatctgt aatttcaggg tgtgacccgt tagaagttac tggtataggt gcaggtctta
    12361 agaaagaaag actaaagcat aaggctgaag taattagaaa ggctattgaa attaataatc
    12421 caaatccaac tgatggagta gatattttat ctaaagtagg agggtttgaa ataggctcaa
    12481 tggcaggtgt tatattaggt tgtagtgcta ataggattcc agtagtaatt gatgggttta
    12541 tttcttatgc agcagcatta attgcatata agataaatcc aaaaactaga gaatatatga
    12601 tagcatctca tttatcagca gaatcaggaa ctaaaagagc attagacata ttaaaattag
    12661 accctctatt aaatatggat atgagattag gtgagggaag tggagctgct ttagcatttg
    12721 atataataga agcttcaaac tatacttata aaaatatggc aacttttgat gaaattgaca
    12781 tgggaagata aatataaaaa tttaatatgt aggagtgttt tgatgagtaa aattattcta
    12841 gtaacaggag gagctaggtc agggaagagt aactttgcag aaaaactatg ttttgacaga
    12901 aataataata cagcttatat agcaacctct attccatttg atgatgaaat gaaagataga
    12961 gtaaaaaaac atcaagagaa tagacctaga aactggaata cttatgaaat atatgaagat
    13021 atatattcca taattaagga gatatataaa gaacatgaaa ctgttatact agattgtgta
    13081 acattattag taaataatct aatgtttgaa cacaatctaa atatagaaga atctagtcaa
    13141 gaagaaataa acaaattaga acaatacata aaagaacaag ttgtcaaact tattgaagaa
    13201 atagaaaaaa caaatctata ctttgtattt gtaacgaatg agttaggtat gggaattgtt
    13261 cctgggaata aacttagtag aatttatgct gatattgtag gtcgtataaa tcaatatata
    13321 gcttcgaaaa gtaatgaagt ttattttgta gttagtggca tagctatgaa gataaaggag
    13381 tagtatgcaa ttatgaaaag atttatttta atactacagt ttttgacaag gatacctata
    13441 aaattaaatg taggatttga tgatgaattt tataagtcaa tagtatattt tccacttgta
    13501 ggttttgtca taggtatatt atcctattta ataggatgga tttctatgct tttatttgaa
    13561 ccatttatag cttctataat aataactcta gctggtgtct taattactgg aggacttcat
    13621 atagatggat taggagatac atttgatgca atatatagtt atagggataa agaaaagatg
    13681 cttgaaataa tgaaagattc aagattaggt acaaattcac ttctagcaat tatgtttgta
    13741 cttttattaa aggttggatt cgtttacgat ataataagta ataattcatt gtgggttata
    13801 atttttatgc caatgattgc aaggttagga gtaatgttat tgacatataa aactgtgaca
    13861 ccaagagaaa aaggaatggg aaatttattt ataggaaaat taactacaag tatgttaata
    13921 actgctataa tatacacatt attgatagtt gctcttatta ctaaatttat atttttatta
    13981 cctaatatag tgttgattaa agtattaggt tcgattatag tggtttttgt atttataata
    14041 ttattcaaaa aacatattta taaaaaaatt gacggagtaa caggagatat attaggctgt
    14101 ggaatagaac tttcagagct tgtatattta atttacatat atttgttaat ttttatgttt
    14161 ttttaaagta aaaagtggtg ataatatgat aagattaata ttaataagac atgcactgac
    14221 aaatgataat aaaaaaggaa gattatcagg ccatataaat agttgtatta gtgaagaggg
    14281 caaactacaa ataaataaaa ttaccagata tctgagtaat gaaaacatag ataaggttta
    14341 tacaacacca tctacacgta ctaaagatac agtagaaaaa atatcaaaat tgaaattatt
    14401 agaaatagaa gaaaaagaag ctttaagaga aattagtttt ggagactttg aaggaagaac
    14461 ttttgaagaa attaaaatca aatatccaaa tgagtttgaa aaaatgataa aagaaggtaa
    14521 caactataga tatccaaatg gagaaagttt gatagactct tataaaagag tggcaaaaga
    14581 aattgataat ataattttag aaaataatag taatttagat acaaagacaa tacttatatg
    14641 ttctcatgca ggaacaataa gaaatataat aacacatttg atttctggta gttataagta
    14701 tcactggaac tttaaaattg ataatgcatc tattacagtt ttagaaattg atggtggatt
    14761 tgctgtaata gataaaatga attttacaga cttcatataa tttaatcaaa accaaacatt
    14821 acaatctgtg tttgtaatat atctgtaaaa ttagcaatga aaatagaata ttagattaaa
    14881 attaggttct taattagatg ttaaaaggga aaaaggttag atgcctttgc agcccccgct
    14941 actgtgaaac caacgaaact ataaatacca ctgtcagttt gatgggaagg ttatagaagt
    15001 aggatgaagt taagccagga tacctgccta atttaattta aaacaagagg tcttcggggt
    15061 gaaagatttt taattaatat atataaaagt cctatcttta gaagtattag gacttttttt
    15121 aatttgtttt ttagtaaatt ttaacaagga gggatggaaa gtggggaaaa aaataatgct
    15181 acaaggaaca gcttcaaatg taggtaaaag tgtcttagta gcaggtttgt gtagaatatt
    15241 taagcaagat ggttatactg ttgctccttt taagtcacaa aatatggctt taaattcttt
    15301 tataactaaa gaaggattag aaatgggaag agcacaagta tttcaagcag aggctgcgaa
    15361 aatagagcca atagctgata tgaatccagt tttattaaaa ccttctggaa ataataaaag
    15421 tcaagttatt gtaagaggta aagttgtagg agaaatgcct tcttctaagt atcatgatta
    15481 taaattggag ttagtagatg tgttgaagga aacatttaat agcttaaatt caaggtatga
    15541 tattgtagtt atggaagggg caggaagtaa tgcagagata aacttaaaag atagagatat
    15601 ctctaatatg ggtatggcag aaatagctga tgcacctgta ataatagtcg gagatattga
    15661 tagaggaggc gtatttgcct cattagcagg aacaatgctt ttattaaatg aagatgagaa
    15721 aagaagagta aaaggtgtaa taatcaataa attcagagga aaaaaagagc ttttacaaga
    15781 tggtgtaaag atgctagaag acatcataaa agtacctgtt ataggagtag taccttatac
    15841 agatataaaa atagaagatg aagacagtgt aactacgaga tttaagaaaa aaatgaataa
    15901 aagtgatata cacatagaaa taattagaac cccacatatg tcaaatttta cagattttaa
    15961 tatatttgag acacaagaag atgtaagtat aagatatgta gactatggag aatctttagg
    16021 aaatccagat atattaataa tacctggaac taaaagtact atagacgatt taacatatat
    16081 aagaaaaagt ggtcttgaat cgcaaatcaa aaatttacat agtcaaggta aattgatatt
    16141 tgggatatgt ggtggatacc aaatgttagg taaaaaatta aaagaccctt atcatgttga
    16201 aagtgaaatt gaagaagttg atggaatagg tcttttagat acagaaacaa cgtttgagaa
    16261 agaaaagact acaacacaag tagaagctac tattgttgaa tgtatcagtg aatacatgaa
    16321 taaccttaag ggaaaaaaag taaaaggtta tgaaattcat atgggaatta caaagcgagg
    16381 taatgatgta agaagtttag atttaataac taaaaaactt gataaagaag tgaattatac
    16441 tgagggaagt gtaaataaag aggggaatgt aattgggact tatcttcatg gaatatttga
    16501 tgaaataagc tttacaagag caattttaaa caatataaga aagaaaaaag gtttaggaga
    16561 attagaaagc aaagtaaact cttttaatga atttaaggat aaagagtatg ataagttggc
    16621 tgatttttta agagaacatt tagatataga gaaaatatat gagattatga aataatgatg
    16681 acctttattt ataatttagg taggaggtgt attatgaaaa aaattttaat tgcgggtact
    16741 aatagtggag taggtaaaac aacaatatca ttaggaataa tgcaagcttt ggttaagaga
    16801 aatatgaaag tacagccata taaagtaggg ccagactata tagacccttc atatcacact
    16861 tttataactg gaagacattc tagaaattta gattcttata tgttagatga tgaaaaaata
    16921 aagtatatag taaataagtc ctcaaaagat gcagatattt cagtaataga aggtgttatg
    16981 ggcttatatg atggttttgg aatagattta gataattgta caagttcaca tacttcaaag
    17041 gtattaaagt cacctgttat attagttata aatggtaaat ctatggcagc atctagtgct
    17101 gctatggtac ttggatataa ggaactagat aaagatgtaa atataaaggg tgttatagta
    17161 aataatgtaa agactaatag ccattatcaa ataattaaga gttctataga aaaatattgt
    17221 aatgttgaag tattggggta ttttcctcca aataacaaat tctctttgga atctcgacat
    17281 ctaggattag ttcctagtgt agaaatgaaa tcactaaggg aaaaatttta taccttagca
    17341 gatgaaattg aacactatat agatattgac agaataattg aaatatcaga aagtgatgaa
    17401 tttgagagtg attttgattt tcaaactttg atagaaaata ataaaataaa agaacatgtt
    17461 aataataaat caatagctat agcatatgat aaagcattca atttttatta tagagaaaat
    17521 atagagcttt tagaggaact tggtttagaa ataaactatt ttagccctct tgaagatgca
    17581 tcagtgccaa atgcagatta tatatatatt ggaggaggtt ttccagaaat atttggaaaa
    17641 gaattagaag caaatgagtc aatgagaact tccatattag aagcacataa caataatata
    17701 cctatttatg cagaatgtgg aggactgatg tacctaggtg agaaactatt gaatcaagaa
    17761 gaacaagaat ttaatatggt tggaatattt agtggaatta gtaagatgac atcttcttta
    17821 aagagatttg ggtactgtat tggtgtagcc aaggaaaata ctcttttatc taaaaaaggg
    17881 gaatctatta aagggcatga gtttcatcat tcaatatttg aaagtgatga aaaatgtgta
    17941 tattcaatga gaaaagaaag agatggaaat atagtagaag agtgggatgg tggttatagt
    18001 aaaggaaaca ctttggctac atatcttcat actcattttt ataataactt aaattgtata
    18061 gaaaattttt tgaaaaaagg aaatgaataa atgagtattt tatctattta tataggatat
    18121 gtgacagact taattgtagg agacccatat tcttttcctc atccagtgag atttattggt
    18181 aaacttataa attttactca atgtataata agaaaggtct ctaaaaatga taggcagtta
    18241 aaatttgggg gatttatatt atggcttata accgttggaa taacatattt aacaacttat
    18301 gctatagtga agttatttag ttttaatata ttattgagta ttgtagtgaa tagttttata
    18361 atttatacaa cactagccac aaaatgttta aaagacgaag cattaaaaat ttataatgta
    18421 ttaaaaactg aagatataga aaaatctagg attcagctat cttatatagt aggtagggat
    18481 actactaatt taagtgaacc tgaaataata agagcaactg tagaaactgt tgcagaaaat
    18541 actgtagatg gtataattgc gcctatgttt tatgcattta ttggaggagc accacttgct
    18601 atggcctata aagctatcaa tacattggac tcaacagttg gatataaaaa taaaaagtat
    18661 aaagatattg gttttgcatc tgctaagatt gatgatatag ccaattatat accagctaga
    18721 atatctgtca ttcttatgac aattggaagt ttctttttaa attataacta taggaactgt
    18781 tttaaaatct ctattagaga tagaaagaat cataaaagtc caaattgtgc tttttcagaa
    18841 ggtgcagtat ctggtgctct aggaatacaa ctaggaggta ccaacgttta ttttggggaa
    18901 aaagtatata agccaacaat aggggataag cttagggaga tagataaaga agatataatt
    18961 aaaactaata aaattatgta tgcatcttca tttgtatcta tgttggtatt tacactaata
    19021 tatgtgttat gtaggatgat tttaattaac ttaattatat aaattattgt ttgttaactt
    19081 aagggggaca tagatgaagg atttagggca tggtgccaat gtagatgaaa tggcaagatt
    19141 atatgggaaa aacccaaaag aaataattga ttttagttca aacataaatc caaatgtatt
    19201 accaaattta gagagataca ttttaaaagg attagaagag tgtaggaatt atccagatat
    19261 aaattataca aacttgagag aaaatatatc aaaatatata gatataaatc cagactttat
    19321 aatacctggc aatggtgcaa cagaagtgat ttatttgctt atgaagagta ttaaaaaaaa
    19381 attggctata ataaatccga ctttttcaga gtatagaaga agtgctgagt taaataattt
    19441 agatattata gatttagaat tagatttaga aaataacttt aaacttaata tagatataat
    19501 aaaagaaaat ataaaaagat ttgatagttt atttatatgt aatcctaata atccgagtgg
    19561 taatgtccaa gatttaaagg aacttgttca tttactagat aaacataata aggtactaat
    19621 aattgatgaa actttcatgg aatttgttga agatgaaagt aaatatagtt tggttaaata
    19681 tatagaatca aataaaaata tatttataat taaagctgtg actaagtttt ttggtatgcc
    19741 tggtttaagg ctaggatatg gtcttacaag caacactgaa attatgaaca aaatttatga
    19801 acacaaagag ccatggacta tcaattcttt tgcagatata ctatccaatt ttatttttga
    19861 agataaagag tatattaaaa atagtaaaga atattacata gaagaaagaa aatatatgtt
    19921 acaagagttg agaaacataa gaaatataaa ggtctatgat acagatgcta attttatctt
    19981 gataagaata tataaaaaaa caactaaaga attgaaaaaa gatttgttta agcaaggaaa
    20041 catattagtt agagatgcgt ctaattttat aggattagat gatagtttta taagagtggc
    20101 tataaaatct catgaagata acaaaattct tatagaaaat atgaaaaatt tattgggtga
    20161 ttaaaatgaa atcttatgga atatgcccag catcttgtgg agaatttgta caaggtataa
    20221 ttgatgatga agaatattta tgttcatatg caatagatat gtattctaaa gtgtatattg
    20281 aagaaaaatt agtagatata aatttaggga gatataagtc aaggttagca attgaaaagg
    20341 tatttgaaaa atttaattta cctaaaaaat atactaaaaa tatttcttta aatataaata
    20401 gtaaaatacc tgttggaaaa ggaatggcaa gttcaactgc tgatatagga gctactataa
    20461 aggcaacatt atcattgata gataaagatt taagtagcga ggagatttct aaattagcag
    20521 cagaaataga accaacagat tctatattta tagataaaaa tagtatattt aatcctctaa
    20581 atggaactgt tataaaatac ctaggaaatc tgaccaatgc gaaagttgta atacttgaac
    20641 caaacaaagt actagatact atgaagatta gattgaggca agattataat aaattaaaag
    20701 tagagaataa agaagtaata aaaaaatcat ttgctctttt agaagaagga ttgaaaaaaa
    20761 ataatttatc tctagttgga gaggcttgta ctttgagtag cttagcaaat gagaatatag
    20821 aaaaaaaaga gtgtttaaat gaaataataa aaatatcaaa aaaatatgga gcatatggag
    20881 taaatattgc tcacagtgga actgtagtag gtattttaat tgataagtct atgaatgata
    20941 aaaagatgat agatgcctta tgtgaatcta acataaattc tgtctataat aaaatttata
    21001 cacaaaacat aattaatgga ggaattaagg gggaaataga atggaataca taaagaatcc
    21061 tatgaaaata gaagaaaaaa gttttgaatt gatacaagaa ataatagacg aaattagacc
    21121 agattataaa tttaagaata atatcgaaga aaaaataata aaaagagcta tacatactac
    21181 tgctgatttt gattacttag atatattaaa aatatcagaa gaagcagttg atagtataat
    21241 agatgcatta aaaaataatg caagtattta tactgataca aatatggctc ttagtggtat
    21301 aaataaaaga aagttagaat cattaggatg taagtataaa tgtttagtgg cagatgatga
    21361 aactgtaaaa ttagctaaag aaaaaggaat tactcgttct atggcagcag tagaaattgc
    21421 agctaaagaa gaaggaagaa aaatattcgt tttaggtaat gctccaacag ctttatataa
    21481 agttatggag atgaaatcag aaggtagatt agatttagat gcagttatag gagttcctgt
    21541 aggttttgta ggagcacaag aatcaaaaga tgaagttgaa aaaacagata taccatatat
    21601 aatttctaaa ggtagaaaag gtggcagtaa tttagcagca gcaatagtaa atgctatatt
    21661 atatactatg taggtaccta tatggaagaa tatgtatata tagatggaaa aaaatataga
    21721 agaggatata ctacaggttc atgtgcaaca ggtgcatcta aggctgctgt atatatgttg
    21781 attacaaaaa atagaatcaa tactataaat atagatactc ctaaggggat accattacta
    21841 ctaaaagttg ataatataaa tatttctgat acatttgtgg aatgttccat aaaaaaagat
    21901 ggtggagatg atatagatgc aactcatacc atggatattt atgcaagagc tgaaattgta
    21961 gctaaaaatg ataagaacaa agggtatctt actctaaagg atatagatag tttaagcacc
    22021 aatagtgagt gtaaaagtga gctctataag tttattaggg tttatggcgg aactggtatt
    22081 ggtgtggtta cgaaaaaagg gcttagtgtg gatgttggta agcctgctat aaatccgact
    22141 ccacttaaaa tgataaatca tgaaataaga aagcttatag gagataattt tgaatctata
    22201 cttggaaatg ataaagtttt aaaaataaca atctttgctc cacaaggaga aactgtagca
    22261 aaaaaaacat ttaatccaag attaggtata gttggaggaa tatctataat aggtacaact
    22321 gggattgttg aacctatgag tgatgagggt tggaagaagt ctctatctat agaacttcag
    22381 atgaaaaaag aacaaggctt agataaaata atattggttc caggaaatca tggagaacag
    22441 tttataagag aaaaattaaa cttagatatt aagtatgtag ttagggtaag taattttata
    22501 ggctatatga ttaaagaagc tcaaagaata ggatataaga aaatcttaat ggcaggtcat
    22561 ataggtaaat ttataaaagt atctgctggt atttttaata ctcatagtaa ggtagcagat
    22621 gctagaagtg agatattagt ggcaaactta gctttaatgg gagctagata cgaattttta
    22681 aataaaataa atcaatgcgt aactactgaa gaagctgtag agctaataaa taatagtgaa
    22741 tatagagaag tatataatat attaagtaat aaatgtaggg agagagtaaa gcaatacttg
    22801 aatgaagatt cagatgatat tgatgtggaa gtaatcatat tttcaatgga taagtcttta
    22861 ttaggtaaat cggataatac agatgattta gtggaggtct ttatatgata aacataatag
    22921 gtctaggacc tggcaactta gactatataa caaaaaaagg tgaaaattta atttccactt
    22981 ctgatgtatt aattggtggt aaaagaaatt tagagtctat aaaaaatttt gaaggagaaa
    23041 aaatagttct ggattctaat ttaagagaaa ttatagagta cataaataac aataaagaaa
    23101 agcaaatatc aataatagca tctggggacc cattaatata tgggataggt agatatctat
    23161 ctaaaaatat tgataataag atgttaaaca tggtttctgg gattagttca ttacagtaca
    23221 tattttcaaa aatatatgta gatatgaatg atgtatatat tacaagtagt catggtaaag
    23281 taccagattt tgattacata ttatcacata aaaaagtatg tatggttaca gattcaaaga
    23341 taggaccaaa acaaataagt agagaaataa tagatagaaa tctaaataaa attattgttg
    23401 taggagaaaa tctgtcatat gacaatgaaa aaataacaat agcaaaacca gaagagataa
    23461 taagaataga caattttgat atgaatgttg tggtgatttt agatgaggaa tagtgaattt
    23521 ataacaggta aagttccaat tactaaggaa gaggttagag caatatcaat aagtaagtta
    23581 aatttagtaa atgctaaaaa cctcatagac ataggggctg gtacaggaag tgtaagtgtt
    23641 gaagctgcat ataattatcc taatttaaag gttatatcta tagagaaaaa tgatgatgct
    23701 atagagctta tagagaaaaa tataaaaaaa ttcaacttaa aaaatataga agtgataaaa
    23761 ggatgtgctc caattaatct agggttaaat gttaaagttg agtctatttt tcttggggga
    23821 acaggaaata atctggaaga aataatagaa tggtctaaaa aaaatctcgt tacaggagga
    23881 agacttgtag ctaattttat aataatagat acatttaatc aaactttaaa actgcttaaa
    23941 aagcatggat ttaaagaaat agatgtatgt gttttgaatg tttctaaact ggagaaacta
    24001 ggaaaaggtg aatattttaa accactaaat cctatatata taatatcttg tgaaaaaggg
    24061 gaaaatgatg atgaataaag tacattttgt aggagcagga cctggagata aagagctgat
    24121 aacattaaaa ggatacaaat tattaagtaa tgcagatgta gtgatatatg caggttcact
    24181 tgttaatcct gaacttttgg aatactgtaa agaagattgc caaatacata atagtgcaca
    24241 tatggattta caagagataa tagatgttat gagagaaggt atagaaaata ataaatctgt
    24301 tgtaagactg caaacaggtg atttttctat atatggttca attagagaac aagtggaaga
    24361 tttaaataaa ttgaatatag attatgactg tactcctgga gtaagttctt ttttaggggc
    24421 agcatcatct ttaggggttg aatatacagt tccagaaatt tctcaaagtg taattataac
    24481 aagaatggaa ggcagaactc cggttccaga gaaagaatca atacaatcat atgctaagca
    24541 tcagacatct atggtaatat ttttatctgt tcaagaaatt gagaaagttg tgtctaaatt
    24601 actggaaggt ggatatccaa aagatactcc tatagcagtt atatacaaag caacttgggc
    24661 agatgaaaaa atagttaaag gaactttaag tgatatagct gtaaaagtta aagaaaacaa
    24721 cataaataag actgctttaa taatggttgg gagattttta ggagaagagt ataataattc
    24781 aaagttatat gataaggatt tcaaacatga atatagaggg taatattgct ataatctgta
    24841 ttacagaaaa tggaaaaaaa ttagctctaa agataaattc tctagtaaat aactgtgtta
    24901 tatatcaagt aaaaaataaa aaaagtaatt tagttgttga agaaacatgt gtacatacag
    24961 ttcaaaagaa gttaagtgat tttgtaggag aaatatttag taaatttgat tacatagtat
    25021 ttataatggc tacaggtata gttgtacgaa gtatagcacc atacataata agtaagttta
    25081 gtgaccctgc aattttggta atggatgaaa aaggcaaaaa cgtaataagt ttattgagtg
    25141 gtcatattgg aggggcaaac agcttgacac agtacataag tagtcttatt gatgctaatc
    25201 cagtaataac aacagctact gatataaatc aaaaagcttc cttagatatg atagcaaaaa
    25261 aactaaatgc acatattgat aattttagag aaagtgtaaa agaagtaaat tctatgctag
    25321 tgaataataa atgtgttgga ttatatgttg atgaaggata tcaagttgat actaggggat
    25381 ttaaacttat agaaagtaag gatgagttaa gagatagttt ttcaaacaca tatatagaaa
    25441 aattagttgt agtaagcaat aaaataaatt gtattgaaga tattgagaat ttaaacctag
    25501 ataataaatt tgaacattgc tatagtaccc aaaaaataat aaaggtaatt ccaagagata
    25561 tagtgatagg aattggctgt agaagaaaca cagatagtga actgatgtat aattcacttg
    25621 aagagtttct taataaacaa aatatagata ttagagcaat aaacacagtt ggaagtatag
    25681 aactgaaaaa agacgaaaaa gcaataattg aattatcaaa aatattgaag gtgccattta
    25741 aaacattttc agtagatgaa atatctaagg ttgataatct ttttgataaa tctgattttg
    25801 ttaaaaaaaa tgtgggtgtg tattcagtag cagaacctgt agcatatctt ttgagcaaag
    25861 gtaatttgtt agttgaaaaa cataaatata aaggaataac tttttcagtg gggagaataa
    25921 aaatatgata tacgttgtag gaattggacc aggaagtaaa gatacaatga ctttagaagc
    25981 aataaaagca atagaagatt ctgaggctat tgttggatat aagacttata taaaactaat
    26041 agaagaattt atacaaggta aagaaataat ccaaaatggt atgaggcaag aagttgatag
    26101 atgtaaacaa gcagtggagg aagcaaaaaa aggaaaaaaa gtagcagttg taagcagtgg
    26161 tgatgctgga atatatggga tggcaggtct tattttagaa ttaatatcta aagaagatga
    26221 agatataaaa gtaaaggtaa ttccaggcgt tactgcaagt attggtgcag cagctatact
    26281 tggagctcct attatgcatg atttctgtca tataagtttg agtgatttaa tgactccgtg
    26341 ggaagtaatt gaaaaaagat tgagattggc tgctgaagcc gattttgtaa tttgtttgta
    26401 taatccaaga agtaagggaa gaagtgaaca tctaagcaaa gcattcaaaa taatgggtga
    26461 gtttaaaggt ggagatacac ctgttggagt ggtaaaagat gtaggtagag aaaaagaaga
    26521 aaagtttgta tgtacttttg aaaatatgga ctttgaaaaa gtcgatatga ctacaatggt
    26581 tataatagga aataagtcta cctatattaa tggtgaattg atgataactc caagaggtta
    26641 tacagtatga ttttaatttt aggaggaact tcagatagtt tatctatatg tgataagata
    26701 aatgaactga aaaatcaacc atatatcctt tcagttacta cagattatgg aagggaactt
    26761 gcacaacaac atgcagagaa tgtaatttta ggtaagcttg gaaaagaaga tatgctaaaa
    26821 tttataaaag ataataatat agtaaaaata atagatgcca ctcatcctta tgcagttgaa
    26881 gtatccaaaa ctgctattac ttgtgcaaaa ctattgaata tagattatat aaggtttgag
    26941 agaaaatctt taattgaaga aatagattat gataataaat ttatagtaga tacaattgaa
    27001 gaagcctgtg aaatagcaaa taaaataggg acaaatatat tcataggaac aggaagtaaa
    27061 aatttgaaca gatttataga ggaaataagt gataaaaatt tagttgttag agttcttcca
    27121 acgagtgaag tcatattaag ttgtgaaaaa ttaggtttaa gtgcagataa tatagtagct
    27181 atgaaaggtc catttagcca gtatataaat gaagaaacat ataaacatta caatatagat
    27241 ttagtcataa caaaagaaag tggaatagct ggtgggttct tagagaaggt aaatgcatgt
    27301 aaaactttca atataccagt aataattata aaaagagaat cgattaacta cgaaaatgta
    27361 tttaatgaca taaataaaat taaagacata cttgtgtaat ttttaggagg agaatatatg
    27421 aagaaagcct tattagtagt gagttttggt acaagctatc atgaaacgag agaaaagaca
    27481 atagatatat gtgaagataa aataaaaaat tcgttaaaaa gccatgattt ttttagagct
    27541 tatacatcaa atatgataat aaataaaata aaaaaaaggg atggaataga aatagataat
    27601 ccaatacagg ctttagataa aatatatgaa caaggatatg atgaagtaat aatccaaaca
    27661 cttcatatta tatgtggcga agaatttaac aaattaaaag aacaagtaga tggatattct
    27721 tctaaattca aaaagattgt attaggaaga cctttgctta cacatattga agattatcag
    27781 gaagctgtag aggcaattaa gcatcaaatt cctcatatgg aaagtaatga agcagtagta
    27841 tttatgggac atggaacact tcatgaatca cattcagcgt atccagcatt ggaatacatg
    27901 cttagggata atggaataaa tgcttatgtt ggtacagttg aagggtatcc agaaatagaa
    27961 catgtaataa gaagattaaa agaaggaaat ataaaaactg taaatttgat gccatttatg
    28021 ttagttgcag gagaccatgc tataaatgat atggcaggag atgaagaaga ttcttggaaa
    28081 acaatattag aagaaaatgg atttaacgta agagttcatt taaaaggact aggagaaaat
    28141 tcttatatac aagataaatt tgttcgtcat gctgtaaaat gtgcagagaa tgcgaagttc
    28201 tatggtatag gagcagggcc aggagatggt tctttactta cgataaaagc ggttaatact
    28261 ttaaagaaac tggatattct atacactcca gaatcaaaaa agggtggaga tagtctagct
    28321 ttatcaatag ttagtgaata tttacctgaa tcagtggaaa taaagtctag gcatttccca
    28381 atgagttttg atgggaatga aaaaagttta gcttgggaca atgtggcaca agaaattata
    28441 tcagatgtta atgatggtaa aaatgtagga tttgttacat taggagaccc tatgatttat
    28501 agtacatatg tatatataat gaaaagaata ttaggagagg tagaagtcga aactatacca
    28561 ggaatttcat cattttccaa tatagcatct aatcagaatt ttccattagt catggataaa
    28621 gaagctttag tggtaattcc ttgtactatg gaagatgata agatagagta tgctttgcaa
    28681 aattataatt caatagtatt gatgaaagta tataaaaatt ttaaagaaat aataaaaaaa
    28741 ttagaaaagt atgatttaat agaacatgct attttagtaa gtaattcttc ccaagaaagc
    28801 gaaaatgtgt ttacagattt aaaagaagcg caccttgaag ataagatatc ttatttttct
    28861 actatactag taaataaaga taataaaata aaataggtga aagcaatatt taaaggagac
    28921 ttaatatgaa tatagtggtt ggaactaggg gtagtaatct agcgcttata caaacagaat
    28981 gggtaataaa tgaattaaaa aagaagtatc cagaaattag ttttgagatt aaaatcataa
    29041 agacaaaagg agatttaatt caaaatgttt cactggataa aataggtgat aaaggattgt
    29101 ttgtcaaaga aatagagcaa caattattag atggaaaaat agacatagca gtacatagta
    29161 tgaaagatat gccttcttat ttagcagatg gactaaaatt tgctcatacg cctaagagag
    29221 aagacccaag agatgttcta atacttagag aaggatataa aaatttagat gatttgcctc
    29281 atggagcagt aataggaaca ggaagtaaaa gaaggaagtt tcaattattg aaacaaagac
    29341 ctgatttaaa tatagtacaa gttagaggta atgtggaaac taggatacga aaaataaaag
    29401 atgaaaacat gcatggtatt gtgttggcag catcaggaat aattagagct aatttacaag
    29461 ataaaataag ttcttattta cctgttgatg ttgttattcc agcacctgcc caaggagctt
    29521 tagctataga aattagaagc aatgatagta cgatagaggg aatagtaaat tcactgaagg
    29581 atgagaatac agaaatccaa atattggctg aaagaggttt tttagatggt gtaaatggaa
    29641 gttgtcatat tccaatagca gcttattgtg aaataatcca agataaaata catcttactg
    29701 gcttatatgg agattcagaa ggaaaaaaag tagtaatcaa gtctatagat ggagatattt
    29761 cttctcctag agagttaggt ttaaagttag ctaaacttgt attgaaggag tatgagaatt
    29821 atgaagggta aggtttactt agtaggggca ggaccaggtg attataaact catgacttta
    29881 aaaggtttag agtgtataag aaaatcggat gtaatagtat atgatagatt agcaaatagt
    29941 agttatttaa gagaagctaa gcctgattgt gaacttatat atgtaggcaa ggcatcaagt
    30001 aatcatattt tgccacaaga agatataaat agaattatag cagaaaaagc taaagaagga
    30061 aaaattgtaa ctcgtcttaa aggaggagac ccttatgtat ttggcagagg gggagaagaa
    30121 gcagagactt tagtagatga aggtatagaa tttgaagtag ttccaggtat aacttctgct
    30181 ataggaggtt tatgttatgc gggtattcct ataacacata gagaccatgc ttcgtcattt
    30241 catgtaataa caggtcattt gaagggtgat gatagtggag aattaaactg gaatgctctt
    30301 gcaaataaca aaggtactct tgtgtttttg atgggaattt caaacctaga gaaaataagt
    30361 gaaaacttaa tgaaagaagg aaaagataag gatacacctg ttgctcttat aagttgggca
    30421 actagatata atcaaagagt tgttacatct acacttgaaa atgtttatga aactgctatt
    30481 aaagaagaag taaaaccgcc aactcttata gtagttggga gtgttgtagg acttagagaa
    30541 aagttgaact tctttgaaag taagccttta tttggaaaaa gtattgctgt aactaggtct
    30601 agaaatcaaa atagtgtatt ggtagaaaaa ataatggatt taggtggaaa tcctatagaa
    30661 atacctacta ttaaggtaga aaaaatacaa aataatatta acttagaaaa tgaaataaaa
    30721 aatataaata agtataatta tctaatacta actagtaaaa atgccgtaga aatcttcttt
    30781 gaaaagatat atgaaatgaa ttttgattta aggatattat ctaatttaaa ggtatgtgct
    30841 ataggttctg ctacatcaaa tgagttgaaa aaaagaggaa ttatagcgga tatagtacca
    30901 aaaaaatttg tagcagagtc actgtatgaa gagttagcgc ctattttaaa tgatgacgat
    30961 aatatactta tacctagagc agaaaatgct agagattatc tagttgacaa acttaaggct
    31021 atttgtgaag taacagaagt acatatatat agaacagtaa tagatgagga gaaaaaaggt
    31081 gagatattag atattttaaa ttctaatgac atagattata taacatttac tagttcctca
    31141 acagtaaata atcttgttga aattatagga aaagaaaata ttaaaatatt agaaaaaata
    31201 aaaacactat ctataggtcc tataactaca gagacaataa atagtttggg aataaaattg
    31261 tacaaagaat ctgctacatc tactatagat tcgttagtag atttaattat aaaaaattaa
    31321 ttttggaggc ttgaatatgt taagaagacc aagaagacta agagcaagta aagcaatcag
    31381 aaatttggta agagaaacta aggtgaatgt agaagactta atatatccac tgtttgtagt
    31441 agagggggaa aatataaaag aagaaattaa atctttacca gatgtctatc atttatcaat
    31501 agatatgcta gaagacgaaa taaaagaaat aagtgagttg ggaattgaac atataatgtt
    31561 atttggtgtt ccagatgaaa aagatgagtg tgcttcacaa gcttttaatg ataatggaat
    31621 agttcaaaag gcaattagaa aggtaaaaga aataaattca gatataaatg taataacaga
    31681 tgtctgtatg tgccaatata caagtcatgg acattgtggg attttaactg atactggtta
    31741 tgttgataat gataaatcat tggagtattt ggctaaaata tctgtaagcc atgctaaagc
    31801 aggagctgat atggttgctc catctgatat gatggatgga agaatacaag caataagaga
    31861 agctttagat gaaaatggat tagaaaatgt tggaatcatg gcttatagtg ttaagtatgc
    31921 ttcaaattat tatggacctt ttagagaggc agctcattca gcaccatctt ttggagatag
    31981 aaaaacatat caaatggacc cagcaaatag caatgaagct ataatagaga ctgaacttga
    32041 tatattggaa ggtgcagata tacttatggt taaacctgca ttatcatatt tagatattat
    32101 aagaagagtt aaagataatt ttgacttacc tctagcagct tataatgtaa gtggagaata
    32161 ctctatgctt aagtttgctg tgaaggaagg tttattaaat gaagatgtaa ttcttgaatc
    32221 aattacatct ataaaaagag caggagcaga tataataata acttattttg ctaaagacat
    32281 tgctaaactg ttgaaaaaat aaattctagt agtataaaga atatcaaaaa gtataggaat
    32341 tgtaattaac tattatacag tagttttgtt tagacattta ttgaagaata atagaaacta
    32401 atttgcaatt tctatacttt atttatgtaa ctaaataaaa tttggaggta gaagatgcta
    32461 tatcctatta atttaaaatt agatgagtta gatgtagtta taattggtgg tggagaagtt
    32521 gcatatagga aatgtaaaaa tttc
//
LOCUS       DAELNY010000025        31108 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.025, whole genome shotgun sequence.
ACCESSION   DAELNY010000025 DAELNY010000000
VERSION     DAELNY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.025"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..289
                     /locus_tag="KPE55_001027"
     CDS             <1..289
                     /locus_tag="KPE55_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG0492123.1"
                     /translation="KILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRNDYWIF
                     NPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(431..691)
                     /locus_tag="KPE55_001028"
     CDS             complement(431..691)
                     /locus_tag="KPE55_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG0492124.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            908..2191
                     /gene="hflX"
                     /locus_tag="KPE55_001029"
     CDS             908..2191
                     /gene="hflX"
                     /locus_tag="KPE55_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0492125.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVRLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            2199..2876
                     /locus_tag="KPE55_001030"
     CDS             2199..2876
                     /locus_tag="KPE55_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG0492126.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2962..3405
                     /locus_tag="KPE55_001031"
     CDS             2962..3405
                     /locus_tag="KPE55_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0492127.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3710..4354
                     /locus_tag="KPE55_001032"
     CDS             3710..4354
                     /locus_tag="KPE55_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG0492128.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4440..4817)
                     /locus_tag="KPE55_001033"
     CDS             complement(4440..4817)
                     /locus_tag="KPE55_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0492129.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4817..5203)
                     /locus_tag="KPE55_001034"
     CDS             complement(4817..5203)
                     /locus_tag="KPE55_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0492130.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5382..6137
                     /gene="nadE"
                     /locus_tag="KPE55_001035"
     CDS             5382..6137
                     /gene="nadE"
                     /locus_tag="KPE55_001035"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG0492131.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            6220..6939
                     /locus_tag="KPE55_001036"
     CDS             6220..6939
                     /locus_tag="KPE55_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0492132.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(7106..8110)
                     /locus_tag="KPE55_001037"
     CDS             complement(7106..8110)
                     /locus_tag="KPE55_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0492133.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8376..9272
                     /locus_tag="KPE55_001038"
     CDS             8376..9272
                     /locus_tag="KPE55_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG0492134.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     CLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9409..10671
                     /locus_tag="KPE55_001039"
     CDS             9409..10671
                     /locus_tag="KPE55_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492135.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10747..11940
                     /locus_tag="KPE55_001040"
     CDS             10747..11940
                     /locus_tag="KPE55_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0492136.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDKHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12569..13363
                     /locus_tag="KPE55_001041"
     CDS             12569..13363
                     /locus_tag="KPE55_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0492137.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13458..14798
                     /locus_tag="KPE55_001042"
     CDS             13458..14798
                     /locus_tag="KPE55_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0492138.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14935..16167
                     /locus_tag="KPE55_001043"
     CDS             14935..16167
                     /locus_tag="KPE55_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0492139.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            16207..17349
                     /locus_tag="KPE55_001044"
     CDS             16207..17349
                     /locus_tag="KPE55_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0492140.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17360..18154
                     /locus_tag="KPE55_001045"
     CDS             17360..18154
                     /locus_tag="KPE55_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0492141.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            18165..19187
                     /locus_tag="KPE55_001046"
     CDS             18165..19187
                     /locus_tag="KPE55_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0492142.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19286..20662
                     /locus_tag="KPE55_001047"
     CDS             19286..20662
                     /locus_tag="KPE55_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0492143.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20994..21338)
                     /locus_tag="KPE55_001048"
     CDS             complement(20994..21338)
                     /locus_tag="KPE55_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492144.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21826..22836
                     /locus_tag="KPE55_001049"
     CDS             21826..22836
                     /locus_tag="KPE55_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0492145.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            22972..23460
                     /locus_tag="KPE55_001050"
     CDS             22972..23460
                     /locus_tag="KPE55_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0492146.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23636..25564
                     /locus_tag="KPE55_001051"
     CDS             23636..25564
                     /locus_tag="KPE55_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0492147.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            25741..26151
                     /locus_tag="KPE55_001052"
     CDS             25741..26151
                     /locus_tag="KPE55_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG0492148.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            26173..26475
                     /locus_tag="KPE55_001053"
     CDS             26173..26475
                     /locus_tag="KPE55_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG0492149.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26692..27384)
                     /locus_tag="KPE55_001054"
     CDS             complement(26692..27384)
                     /locus_tag="KPE55_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0492150.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(27410..28597)
                     /locus_tag="KPE55_001055"
     CDS             complement(27410..28597)
                     /locus_tag="KPE55_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0492151.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            28933..30732
                     /locus_tag="KPE55_001056"
     CDS             28933..30732
                     /locus_tag="KPE55_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0492152.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFD"
BASE COUNT        11730 a         3590 c         5809 g         9979 t
ORIGIN      
        1 aaaaatctta aaaactacat caataacact tataattatg ccattattat taacagttcc
       61 aattttatta aattttgaaa agtcatttat tatttttcat aaattattat ttagaaatga
      121 ttattggata tttaatcccg atttagaccc agttataaat atattgccag aggaattttt
      181 ttttcactct ggaatgatga ttttaatatt aattttgtta gtaagtatag tactgtttgt
      241 aatgtataaa ttatgtaaat ctttatcaat aaaaatattt caatgttaaa tagagggata
      301 tttttatttc caaaataaga taataaaaaa ggattagatt aaaagttata caataaaaat
      361 agctgtgcaa taatatttag actatgaaag tagttgaaaa atatttatgc acagctattt
      421 ataatagaat ttacttttta ctagtgattt taactaaatc ttgaaattta ctcataatgt
      481 aattgtaatt atctttttta tgaggtaata tacaactact gcaatctttg atacctttat
      541 ctgtatactt aaagtttcca ccacagtttt ctcctaaagc atacaaagga caatagcaaa
      601 atagacagtt aaactcttct ggtttattag ttttatgaca agggaaaaat tcgcaatctt
      661 tgtgattaaa aaacttataa ttttcgccca tattttcctc ctaattaaat aaaatttaaa
      721 ttttttatac tatataagat tttggtgata atataattat actaaataat tttatgaata
      781 gtcgataata tagtattatt aatcatagta ttaatggtat aattagtcaa cagaaaaata
      841 atgtaaaaat aatgtaagtg tattttgtaa ttagaatatg ttttataaca aaaaatgagg
      901 tgattaagtg aaagaaattc aagaaaaagc acttcttgta ggtctgaatt tgactacaat
      961 agtgaaaaaa aatgatgata tagacactaa tgaatctatg gaagaattaa aagaactaac
     1021 aaaagctgct ggggcagaag ttgtaggtag cttaattcaa aataaacact ctgtagatgc
     1081 agcttattat ataggaaaag gtaaagtaga agaaataaga gcctatagtg actctttaga
     1141 tgctacttta gtaattttta atgatgaatt atctggtgct caaatcagaa atatagagaa
     1201 tgttgttgga agaaaagtaa tagatagaac tactttaatt ttagatatat ttgctcaaag
     1261 ggcacttagc aaagaaggta aattacaagt tgaattagca caattaaagt atagattgcc
     1321 acgactttat ggaatgggtg gtgagatgag tagaacaggt gcagggattg gtactagagg
     1381 tcctggagaa caaaaactag aaatagataa gagacatata cttaataaag cagcagatat
     1441 aagacgtgaa cttaaagaag taaaaaagaa tagagagaca cagagagtta agcgtttaaa
     1501 gtcaaatata ccgattgtag cacttgttgg gtatacaaat gcaggaaaat ctaccctgtt
     1561 gaacgaatta ataaagacac acaaggatta tgaacaagaa aaagaagttt ttgtaaaaga
     1621 tatgcttttt gcaactttag atgtaacttt gagaaaggct cttttgccga ataaaaaaga
     1681 atttttagta gtagatactg ttggatttgt aagtaaactt ccccatgatt tagtagaagc
     1741 gtttaaggca acattggaag aagtacagta tgcagattta atcttacatg taattgatgc
     1801 aacaaatact agttatgaat tgcaaaaaag tacgactgaa ggtgtattaa aagaacttgg
     1861 tgtaaatgat aaaaaacata tacttgtata taataaagtg gacaagctag aattggatat
     1921 atatcctaaa agtcaagaag atattgtgta tatttctgca aaacaaggca taaatatgga
     1981 taagttatta aatatgatag aaattgcttt aatggaaaat acctattcag taaggttgat
     2041 gttaccttat gagagaggtg atatatttag tagaataaaa gataaatata atgtggagaa
     2101 ttttgagtat ggggaaagtg gtataacgct agatgtagat ttagatgaag aagattttaa
     2161 tatctataga gagtacatac tagaaaaata ggttgaatat ggatataaac tgggaaaata
     2221 tagataatct tgaagattat tttataacct atcttcttta taaagaatct aaaacagtat
     2281 ctcaaattag taaaattaga aatatttcaa gtactgaagc aagtgagcaa cttatacaag
     2341 caaagttaaa aataaaggaa atgcaaaaag atgattttga ggcatctaag gatattttgg
     2401 ataaatttct agaactagat aaaattaaga gacttgattt tatggatagc ttagatgacg
     2461 aaaaaatggt atactttaag agaaaggttt tcaaaagaat ccttgtagaa aaaaatgctg
     2521 aagatttaat tgttttaatt tgggcgacag gtgagctaaa agatgatagg tttttgaaat
     2581 tgcttcatca gctcacaaac cacagacact cagatattag aagaataact tattcagcaa
     2641 tcagaaagat tgaatcacca agtagtagag aagtactgca aaaaggtctc tacgacaaaa
     2701 atgcacagac taggcagtac tgtgcaaaag ctctttcaaa attgggagat gaaaacagtt
     2761 taaagatttt acagcagttg aaagacaaga ataagaattt tgaaaaagaa tatgtgctta
     2821 gagcttatga cgaagccatt aagtcattgg aaaatgcaaa aaatttgaaa aagtaatttt
     2881 ttattaaagc aatttgttta cttaatgatg tatttctaaa gtttaacaag gtaataaata
     2941 aattaatggg aggggtttca aatgatagta agacgttgtg caggtggagt agtgttttat
     3001 gctaataaag tacttattgt aaaaaatgat aggggtgaat ggacacttcc aaaaggaaag
     3061 atactaggag ggggacttcc atatgagagt gctgttcaaa gagttaaagt agaaactggc
     3121 atagatgcta aaatgataga tgtggctggc gatactatgt atgaattttt ctcaagaagt
     3181 agacaacaag aagtttgtaa tgcaataatg tggtatgtta tggaagcttg taacactgat
     3241 tgtgtacttg ctcctgaatt tcaagaaggt ggattttata aagtaaagga tgctttagaa
     3301 atgttatcac atcataaaga gcaagcttta gtagaagtat cttataaaaa gtttaaggaa
     3361 ttaaagaaat tagtttcaga gggcgatgat gttccagcaa attaattgtg aatttagagg
     3421 attctgagga agggatagcc ccttcctttt ttaattgaag cagttttaag cgtaaattat
     3481 atagtctctt taaactatga tttatcaatt tagagattta gtagtttata tttaaaatca
     3541 gtaagatatg tttaaaataa aaattaaaaa gtatacaatt ttattatata attgtcttat
     3601 gatgttgatt aaagaattat taaaagtcca tttattttta aatagaataa ttttataaat
     3661 atatatttga tgtgtatatt agaaaatgat taaaatagga ggaaatacaa tgagcaatta
     3721 caggacatta catgaatttg gaacggatga aataacgatt gaaaagtctg tttttattgg
     3781 atatgctaag cctataaaga gtgaagaaga agcattagag tttataaatg aaataaagaa
     3841 aaagcataaa gatgcaactc acaatgtgtg ggcttatact gtaggtaaaa atatgaatat
     3901 acaaagatat agtgatgatg gagaaccaca aggaacagct gggataccta cattagaggt
     3961 tataaaaaaa gaagatttga gagatgtagc ggttgttgtt actaggtatt ttggaggaac
     4021 aaaattaggt gcaggtggtc tagttagagc ttatacaaaa ggtgctaaac tgggacttga
     4081 agcaggtaag ataatttata aggtaatgta tcaagaagtt aaggttaaga tagattatac
     4141 tcaacttgga aaagtgcaaa atgagttaat gaatttgggg tattttataa aagatactgt
     4201 atatgaggat aatgttgaaa tagtagtata ttcaagatta gaagatgtag agaaattatc
     4261 agaaaaaatg attgatataa caagtggaac aggaaaaata gttttaggag aggaatttta
     4321 tttgtctgaa caaaatggag aaattttaat ataatctaaa aggttattag aatagttaat
     4381 acctcaaata gccaattagt atttttaagt gaaaagccat taaattgatt taatctaaat
     4441 caatttaatg gctttttatc ggattttgca cagaatcctt tttttacttc aaataaaccg
     4501 actaaaagta aaaatatccc aaatattttt tttaatatat ctgaagacag ataattagct
     4561 aatagtgaac caaatacagc tcctaaaacc ccaaacacag ctataggtat aaccaattta
     4621 aaaattacat ttttattttt tatatgtata gcaagtgcta taagtgtcat agggatagat
     4681 gaaagtaaat ttacgctttg tgctatttta gtgtctatgc cagcaagtaa aacgagtgca
     4741 ggtataagga ttgttcctcc acccattccc attcctccaa taattcctgc aaaaaatcca
     4801 attagagcaa ataacattaa aacaccatcc ttaatgctgc aattatcata attattccaa
     4861 aagaaattct aagaaacttt ccagtaacct tatttagtaa tttagctcct ataataccac
     4921 caagaatact tccaactgca actttaattg ttaagttaac atcataagta cctttagata
     4981 catataaaac agaacttgct gtcgttaaga atattattat agaaagagca gttgcatgag
     5041 atttatgttc ttctacctta acgatgtcat ttaatattgg aactaaaaga gtacctcctc
     5101 cagaaccaaa aactccattt ataaatcctg taaaaactcc aattatcgta ttttttaagt
     5161 taaaatcaaa gtattttttc tttaaatcat cagttttttt cataaaaatc accttctgaa
     5221 atagttttac caaaataggc aaattatatt tatataaaat aagattaaat ataatctttc
     5281 atatttgttt aattttgcat aaaaaaaaac atattaaaac aacatattaa tatatggtat
     5341 aatatattaa aacaaagtag caaatttgag aggagataac aatgtcaaat ataaaaatac
     5401 aaatagacaa aactgtagaa tggcttataa ataaggttaa tgaggcaaat gcaaaaggat
     5461 taattgttgg tgtatctgga ggaattgatt cggcagtagt tgctaattta attaaaaagg
     5521 catttccaga aaactctatg ggagttataa tgagcataaa aagtaatcct caagatagag
     5581 aagatgcact taaggttata gagggatgtg atatagaata ccttgattta gaccttatag
     5641 aaccacaaag tgctatatta gacatggtag taggaaattt aaaagataaa catttatata
     5701 gagaagaata tttaaagatg acagatgcaa acttaagagc aagagtaaga atgagtacaa
     5761 tttatacaat tgctaacaat ctaggatatc tggttgttgg tacagacaat gctgcagaaa
     5821 tacacacagg atattttact aaatttggtg atggtggagt tgatatttta cctattgcaa
     5881 atttaactaa aggagaggtg tatgaatggg caaaagagct tggtgttcat gaagatttaa
     5941 taaacaaagc tccttcagca ggtctttggg aaggtcaaac agatgaagat gaaatgggaa
     6001 ctacttacaa tatgatagat gctgtgttag aaggtagatt ggatgaagtt cctaaaagag
     6061 accaagagat aattgaaaga cttcatagat taagtgaaca caagagaaaa acgcctgcac
     6121 aacctcctaa gttctaattt actatttata agatttgtga taaaatttac aagtgatgaa
     6181 aattagaata attaattgta cgtattaagg agtggaaata tgggacgtat aggtaatata
     6241 attaatagaa aaggtaaaca agatgctcaa agagcaaaga tatttactaa acatgctaga
     6301 gcaatagcag ttgcagctaa agaaggtgga gcagatccag agtacaatgc agcgttaaaa
     6361 acagctatag aaaaagctaa ggcagataat atgccaaatg ataatataga tagagctatc
     6421 gctaaaggtg ctggagcagg tgctggagaa gactatgaaa ctatagttta tgaaggatat
     6481 ggacctggtg gagttgctgt tatagtagaa actttaactg acaacaaaaa cagaactgct
     6541 ggtaacgtaa gatattattt tgacaaaaac ggaggaaact taggaactag tggatgtgtg
     6601 tcttttatgt ttgataaaaa aggacaaata ttagttggtt taggagatgg agtttcagaa
     6661 gaagaattga tggatgttgc attagaagca ggagctgaag atttcataac agaagaagat
     6721 ggttatgaaa taataactac accagaagat ttttctagtg ttcgtgatga gttaaaagct
     6781 aaaggatatg agtttatatc tgctgatgtg aagatgatac ctcaaactac aactgtattg
     6841 actgaagaat ctcatttaaa aatgatgaat aaattagttg atatgcttga agaagatgat
     6901 gatgttcaag atatatatca taactgggaa gtagaataaa tagtataagc atattgaaat
     6961 ttataagtat tgaatcaatt ttaacatgtg ataaatttta tagaataaga aacactaaaa
     7021 tagacatacg cacctctaac ctatatttgc ataataatat gtaagcatag gtaaggggtg
     7081 tttttatatt tataggttaa gaaatttaaa gtaaattttg agctggactc gattgtctat
     7141 aaataattga tgggtatata acttgttgct gaataccatt tatacgccca gttactgtat
     7201 caataatcat cctaagacta cgttctgctg tctcttccat ttttaaatct ataactgtca
     7261 aaggaggaat acttcttgcc attagtcgtg aaggtccatt acttgctgca aatatttgaa
     7321 tatcctctcc aattacaatt cccgattcat gaaatgcatt catcatacca agagctacca
     7381 catcatgatt acaaaaaatt acttttttta gtgaattttt tgcaagcatt ttttgagcaa
     7441 tatcatatcc actatctatt ttattagaac aataatatat agaatctgat agatgtattc
     7501 catgttcttt acatgttttt aaaaaagatt taccccttaa agtcatacat gtaaaggaag
     7561 ttgggttgaa tacaacagca atatcatttt taccattttg aatagcatgt aaagctgcta
     7621 tacgaccagt ttcctcatta tcaatagata cacttgaaaa accaggtatt tcacgattta
     7681 caacaactac aggaatttta ggtttatttt ttactagata ttctatatca tcagcattga
     7741 tacctgttaa aacaattcca tcataataat tatttgcaaa aaagtattca tcatcacata
     7801 gatgaccaac tttgtaaggt cgtatgatga tatttatgtt taaatgttct aaagaaatta
     7861 tgtggtttat tgcttgaagt atattggttg atgccaactc aaatccctca tcctgccaat
     7921 aaaaagctat tttaatatta ttatgtttaa taggagtaga gcgtttatat ccaagcttat
     7981 cagctgaatt taatataagc tcttgtgtat gtagcggaat cctgcgttca tttcctttac
     8041 cacttaagac gtacgagaca gttgctgaag aaagaccaca gtcttttgca atatctttga
     8101 ttgttgccat gataaaatcc tccttaaata gttgcttaaa tttgcttata ccaataatct
     8161 taacatatat tcaactaaag aacaatattc tacaagttgc atattttaca ggattagaat
     8221 aaataaattt aaaaaaactt cttacttatg ttgacataat ttagtttata ggattataat
     8281 atgcttaaat aagcttataa ttaacttaaa ttaagcaaag ttaagcaggg acatgcaatg
     8341 ctagcttgca atgtgactgt aaagggggaa aaggtatgag taaggtaaat atatttgaag
     8401 ttggtcctag agatggattt caaaacctga aagagttttt agaaactgat aaaaagctta
     8461 agattattga tggtcttatt gcttcaggga ttaaacaact tgaaattagt tcatttgtaa
     8521 gcccaaaagc aatacctcag atgaaagatg caaaagaagt agcaacttat tgtgtagaaa
     8581 aatatcctga tgtgaaactt tatgcattag ttccaaattt atacggtgct aaagttgcat
     8641 gggagtgtgg tataagagat atatcctatg ttatatcttt gagtgaaact cataataaaa
     8701 aaaatataaa tcgtacccat gaagagtcta ttgatgagct taaaaaaata atgaatactt
     8761 atccagacat gaatatatgt attggaatgg ctactgtatt tggatgtcct tttgaaggtt
     8821 taccaaaaat tgaagatgcc ataagatttg ctaaatgcct ttgggaaaac ggtatcaggg
     8881 agataaatct agcagataca atagggattg ctgaccctaa gcaagtaaga gaaactatta
     8941 gtgcattagt tgaagcttta ccagagtgca gatggcaagt acatattcat gatactagaa
     9001 atatgggaat ggttaatact cttgtggcta ttgaatctgg aattactact gttcagtcta
     9061 cacttggagg attaggagga tgcccatttg ctcctggagc atctggcaat actgcaactg
     9121 aagatttagt atatatgctt gaacgtatgg ggtatgatac aggagttgat tttgttaaac
     9181 ttcttgaatt atcaaagtat cagaagagtt ttgtaaatgg agtatatagt ggtcatcatg
     9241 taaatataaa gtctgatagt tgtaattgtt agttctattg attaatcgat taaaaaatat
     9301 gttgattaaa aaaatatgct gattggtaaa atagatttat tgataaaaaa tataaattat
     9361 aaaatacatt atctaataaa acaaataaaa aaattgaggg ggattagtat ggatattata
     9421 gctttacttt tatttttagg agttattatt attgcatttg tacgtaagaa taatgcaggt
     9481 attttggctc tagctattgg tgtcatagct gttagaattt ttggaatgga tgataaagaa
     9541 ctttatggag ctataagcaa ctcaatgttt actacacttg ttggtataac actactcttt
     9601 gcaatagtga actcaacagg agcattaaag cttgttgcag aaaaaattgt agctgcttct
     9661 ggtaaacgtg tttggcttat acctatagca gtttttatag caggatttgt tgttgctggt
     9721 gttggacctg gggcaattcc agcacttgca attatcccag caatggctgt tcctgtagcc
     9781 ataacagttg gttataaccc tgtaatgctt gcacttatag gtgaatgtgg attaatggca
     9841 ggtcgtatga ctcctattac tccagaaggt caaattataa caaccgttgc agaaggggtt
     9901 ggaattgaaa atgttatgcc tactatctta gcctgtcaaa ctctttctac aattgtattt
     9961 tcaatagtat tgtttataat tttcaaagga tataaactta aaaaacctat aaatgttata
    10021 gatagtaagc aaatagaaaa attcaatata aagcagataa tttctctaat aagcatagta
    10081 gttatgattg cttttatcat aatctttgat atgaatgttg ggcttgcagc ttttgcagtt
    10141 tcagctgtac ttattttttt caatattgct gatgaaggag aatgtatcaa atcaattcca
    10201 tggagcacga tagtaatggt tcttggagta ggtgcaatga tgacaatcgt agatgcagca
    10261 ggaggtattg acttgatgag taatgcatta tcatcactga tgaatagtaa aacagctact
    10321 ccaattatga gtatttcagc aggacttatg tctatggtta gttctgcttt agcagtagtt
    10381 tatccaacta tgatgcctat gtgtgttgat atagctaagg cagttggagg agtagaccca
    10441 ctagcactta ttgctgcagt tggtgttgga ggttctcttg ctggtgtaag cccactttct
    10501 actggtgggg cattgatttt agcagctatg ggttcaagta agaaagactt taataaggag
    10561 gaacaaaata aggtttttgt acagcttttc attatgagtg cagttggctt attggttatt
    10621 gcagtagtat caatattatc atttaatgca ataacacatt taatgaacta attagaataa
    10681 ctgaaaataa atcattaaat ttaactttta atattaatca atataatata atttggaggg
    10741 gaagttatga aaaaaggagc attaagcaat ataaaggtac ttgaccttac aagagttctt
    10801 gcaggtccat attgtacaat gatgttggcg gattttggag ctgatgttat aaaaatagag
    10861 atgccaagaa aaggtgatga tacaagaggt tttggacctt ttgtaaatgg agagagttta
    10921 tactatgcaa atataaatag aaataaaaaa ggtatcactc taaaccttaa atcagaagaa
    10981 ggaaaaaata tatttttaga aatggtaaaa catgctgatg ttgtagtaga aaattacaga
    11041 ccaggtgtaa tggaaaagtt aggtctgggt tatgatgagt tggctaaagt taacaatcaa
    11101 ataatttatg gagctgtatc aggatttgga tgttatggac catattcaga acgtccaggt
    11161 tatgatatta ttgcacaggc aatgggtggt cttatgagta taacagggca agaaggagga
    11221 gaaccaacga gagttggtaa tgctatgggt gatgtactag gtggaatgaa tttaactatt
    11281 ggtatactca cagcactcaa cgctagaaaa ataataggaa aaggacaaag agtagatgtt
    11341 tccctcgttg attctgttgt atcgtctctc gaaactggta tacagagata ctttgattca
    11401 ggacaacctg aacttatggg taatagatat gcttcagcct atccttatga ctcttttaag
    11461 gcaagtgatg gaagttttgt aataggctgt ggtaatgaca aactttttga aaagttatgt
    11521 aaaaaagttc tttttagaga agacctttta atagatgaga gatttaaaac aaatcttaat
    11581 cgttgtgaac attacaaggc tcttaaacca gaaatagaaa aatggtctgt taagcataca
    11641 ataaaagaag cagttgactt aatatcagag gctggagttc ctgctgctcc tatctataat
    11701 cttgattgtg ttacaacaga taaacatata gcaaaagcaa gagaaatgat tgtagatatt
    11761 agtcatccta ttataggaaa tatgaaagtc aatggaaatc cagttaagtt gatgacaaca
    11821 aaagctaaaa ttgatatgcc agcaccatct ctaggggaaa gtaacgagga gatttataat
    11881 ggttggcttg gttttgatag tgataaaata aaagatttaa aagaaaatgg agttatttga
    11941 ttaaaaaata gctaaacaca cttgtgactt tagtcatggg aggttcagtt agaataatat
    12001 caaaaaatat taaatcaata ctgtaaatca aatatagaat tgtcagaagc atttaagatg
    12061 ggaaaagcta cttagacatt gagtctttgt ggcttttcct attttcatta agaattttat
    12121 attatcagaa tagatatgta ctaaactaaa gtattaacaa tttaaattaa tagaaaataa
    12181 actacctaat tatgttgaat aaagactact ttagtgaaaa caaacttata gagatgctat
    12241 ttaaattatt ttaatggaaa tatcaaaaat gagactgcat tctcaaaaat gagactgcgt
    12301 tttttaagta ttttctcgaa aatgagacaa aaatattgtt ttatgggttt tattgaagaa
    12361 aaattcttct gtgaattaca atttaatcaa ttttaaagtt gaaaattatt aaaaatttag
    12421 tgaaatttat gtatatttcg tttattttat taaaaataac aattttttac taaaaataaa
    12481 gaacttcaat ggtgattggc atagtacttg ctattttata ttaacaagaa atgtagaaaa
    12541 aataacaatg tataatgggg ggaatatgat ggaatctgta atggaaaatt tgaacaatct
    12601 taaggtggag gttaaggaca aagtctgtgt tattactata aatagaccta aagctttaaa
    12661 tgctcttaat agtgacactc ttagggaact atcacaagtt attgatgtag tatcagaaaa
    12721 tgaggcaata ttaggagtta ttattactgg agaaggtaaa gtttttgtag ccggtgctga
    12781 tatacgccag atgcaaaact ataaaagtga ggaaggcaga aaatatgcag gttatgctca
    12841 gggaattttt gataaaattg aagctcttga gaaaacagta atagcagcag ttaatggata
    12901 tgcattaggt ggtggatgtg aacttgctat gagttgtgat attagaattg catcagaaaa
    12961 ggctatattt ggacaacctg aagttaattt aggagttatt ccatgttttg gtggaactca
    13021 aagattatca agacttgttg gaactggaat agctaaagag ttaattttta cggggagaca
    13081 agtaaatgca gaagaagcta aatctattgg acttattaat aaagtggttc caagtgattt
    13141 acttttagaa gagtctatga agatgatgaa ccaaatagtt gaaaaagcgc caatagcaat
    13201 aagatatgca aaagttgtta ttaacaaagg gatagatatg gatttaaaga atgctcttga
    13261 actagagaaa gatgttgcag gtttaacttt tgctacaaga gataagcaag aaggaatgaa
    13321 tgcatttata gaaaagagaa agccagtttt tgaaaataaa tagctattat aactaaaatt
    13381 ggaacaatgt agaatatatt caaaatctaa atttcgtgaa aaaatacata tagtttaatt
    13441 ttaaaaggag ttaatttatg aatacgggag taattataag tttagtaggg attatcatag
    13501 ctctatctct tctagttgta atggttatga agggaataaa tatttttatt atagcaattg
    13561 tatgttcatc attagttgct ataacaggaa gcctaaatgt gtatgatgca cttaaagtgg
    13621 attatatgac agggtttgtt ggatttcttc aggcaaattt ctttattttc ctaacaggga
    13681 cattaatggg aaaaataatg gagattactg gtggagcaaa atctattgct aagatgattg
    13741 taagatggat tgggaaggat aaagcactac tttctattcc aatagcttgt ggaatattag
    13801 cctatggtgg agtaagtgtt ttcgttgtaa gttttgcagt gtttccgata gctttagaaa
    13861 tttttaaaga agctgatttg ccacgaagat ttataccagc agctttgact tttggatgct
    13921 caacttttgc tatggttgca ccaggggcac cacaaattca gaatattgtt ccagcatcaa
    13981 cattagggac agatattatg gctggagcag taaatggttt tatatcatgt gcagtaatgt
    14041 ttatagttgg aagtacaatt ttgtacaaaa tggtttcaaa agaaaaagcc aatggtggtc
    14101 attttatagc acatgaatca gatatatttg atgaagcaga agcaactaat tctaagagag
    14161 aaaatggtcc caatggtgta attgccttaa ttccattgat agtgtcgatt ttaataatta
    14221 atgtaaaagt taatggaaaa gcgatagtac caattgaggt gggagtattt attggagctt
    14281 tattagtata tttattgtta aataaatatc aagataatac taaaatagtt ggacatgttg
    14341 gtgatgcatg taaaactaca gtagtagcaa tatgtaatac ttgtgctgta gttgcatttg
    14401 gttcagtagt aaaatcagca gttggttttg acttcatagt taatgctatg actaatattc
    14461 ctggtcctcc tattgcaggg gctgcagttg gaactacagt tattgcaggt atttgtgggt
    14521 cagcttcagg tggactagga attgcagctc cacttcttgg accagtattt ttagccaagg
    14581 gtgtttccgc tgcagcatta actaggacta tggcaatttc atcagcagca ttagattcac
    14641 ttccacacaa tggatatatt gttactgtaa caaatggtct ttgtaaagaa acacataagg
    14701 atgcctatat gccaatattc tgggtaactg ttttaacccc atttattgga accatagtag
    14761 cagtcctact gtttacaatg ttcccgggat tgccatagga gaaaagataa aaggtataaa
    14821 ttatattttg atattataaa atcaattttc aaaatatata caataaattt catgttttaa
    14881 tactgtaaat atgtatttaa aataacaaat tgaccatata aggaggaatt gaaagtgtta
    14941 aataaaccat tagagggagt taaggttatt gacctaacgt attttgtagc aggtccaggg
    15001 acttctaaaa tattagctga ctggggtgct gacgttatta aggtagagcc aagctttgga
    15061 gacccaggaa gaaagacagg ggcaacaatg acaatgccaa tagatgatta taataatcct
    15121 ttttacagca catataattc aaataaaaga ggtttgtcta taaatcttaa gagtgaaact
    15181 ggaattgaaa ttatggataa gcttttaagc gaagcaaacg tatttgtttc aagttataga
    15241 acaggagcat taaaaagact aggtcttgat tatgaatcat taagtaaaaa gcatccacac
    15301 cttatctggg gtcagataaa tggttttggc gattttggac ctgctaagga taatgcagga
    15361 tttgatacag ttgccttttg ggcaagaagt ggggctatgc ttgatatagc agaaaaagat
    15421 acaagtccaa taaatccacc tatagccttt ggagatgcaa caacttcttg tagtttatct
    15481 ggaggaatat gtgcagcttt gtaccagcaa gctaagactg gaaaaggaca aaaagtaatg
    15541 gcatctttat ttgggcaagc aatatggaat gcatcatctt tattagcatc tacacagttt
    15601 agtgatgagt atccaaaaac tagaaaaaat gctatttcac cagtaataaa ttcttatcgt
    15661 tgtaaggatg gaaagtggat attcttaagt attttagagc atgaaagata ttttgaggca
    15721 ctttgtagat tatttggaag agaagactta ataaaagaag agaaatttgc atcttcagta
    15781 gaaagtaaga aaaatgctac agaacttatt aatattttag atggtgaatt tgcaaagttt
    15841 actcaaaatg agatggttga aaaacttact gctgctgata tagctcatga aaaaattcaa
    15901 catgttaatg atgtagtgac tgatgaacag gctattgcta ataactatat atatgaacat
    15961 acaagtaaag atggaaataa gacaatgctt gcatctactc cagttaaatt tggaaatatt
    16021 gaagttaaca tgacttgtga tgctccactt ataggagaac attcagatga gatattgaga
    16081 gaacttggat acagtgattc tgatatagag actctaattg aatctggaat tgtaacaatg
    16141 aataaagaag catgtgaaat atgttaataa acatgtgaag tatgttaata aataggaggg
    16201 aattttatgg attttagatt aacagaagca caattaatgc ttcaaagagt agcaaaagaa
    16261 tttgcagaga atgaaatagc accaatagct gctgaaacag ataaaacagg tatttttcca
    16321 agggagcttt ttagcaaaat ggctaaaata ggatttaatg gaattggaac acctgtagag
    16381 tatagtggtt ctggtggggc agatatagaa aaggtaattg ttgttactga gattgccaaa
    16441 aaatgtgcag catctgcagc aatattatca atacacacaa tatatgctca agctattttg
    16501 aaatttggta ctgaggaaca aaagaaaaaa taccttccaa tgatggctga aggtgggtgt
    16561 gtaggtgctt ttgccttaac cgaacctaat gctggttcag atgctgcgag agctgcaaca
    16621 actgcaatta ttgatgaaga aactgatgaa tatgttttaa atggaactaa gtgttttatt
    16681 tctggtggag gtcaagcaga atcattaata atatttgcat tgacagaccc atcaaaggga
    16741 ataaagggaa tgtctgctat cattgtcgac aaaggtactc caggtttttc aataggaaaa
    16801 attgaagaaa aaatgggtat tcatggttca gaaactgcag agttaatatt tgataactgt
    16861 agagttccaa agtctaactt attaggaaaa gaaggtaaag gatttaatat agctatgact
    16921 tgtttagatg gagcaagaat tggagttgga gctcaagctg taggaattgc tgaaggggct
    16981 ttagaagaaa gtatcaaata ttctaaagaa agagtccagt ttggcaagcc aatttcagct
    17041 ttgcaaggta ttcaatggta tatagcagat atggcgacaa tggtggaatc agcaaaatta
    17101 ttagtatatt atgctgcaga tttgaaagct agaggagaaa aacacactaa agaagctgct
    17161 atggcaaaat acaatgcgtc acgtactgca agagaggtta caaatttagc ccttcaaatc
    17221 catggtggat atggatatat gaaggattat ccattagaga gaatgtacag agatgccaaa
    17281 ataacagaaa tttacgaagg tacttcagaa atacacaaag ttgttatttc aagagcagta
    17341 ctaggatagg aggaaaagaa tgaatatagt tgtttgctta aagcaagttc cagatactaa
    17401 tgaagttaaa ataaataaag agacaggtac tctaataaga gatggtgtac caagtataat
    17461 caatccagat gatagaaatg ctttagaaga agctctaaaa atgaaagatg aactaggagc
    17521 agtgattaag gttataagca tgggacctcc tcaagctaaa agtgctttaa aagaagcttt
    17581 ggcaatggga gctgatgaag cttatttaat ctcagataga gcttttggtg gttcagatac
    17641 ttgggctaca tctacaatta ttgctgctgc tatagagaag gttggaaagt atgatgtaat
    17701 attctgcgga agacaagcta tagatggaga tacagctcag gttggaccag aggttgcaga
    17761 atttttaggg ataccacaag ttacttatgc gaaagaagta aaggttcaag atgacaaatt
    17821 actagtaact cgttatacag agacagggga ttatttaata gaagcaaaga tgccagtttt
    17881 attaactgct ataaaagagt taaacaatcc aagatatcca agtgtcaagg gaatcttaga
    17941 agcttataat aatggagatg caaagattac tgtattaaca cttgcagatt tagatgttga
    18001 tacaactcaa attggattaa agggttcacc tacaaacgtt tataagtctt ttgtaccagt
    18061 taaagacaag cataatgaaa taatagaagg tataaataaa aaagaaaaag ctgagaaact
    18121 aatagaaata ctattcgatt tgaaactagt ataggagtgt taagatgatg agagcaaaag
    18181 ttaatcaagg tataaattta aatgattata atggtgtttg ggtaatagga gagcaaaggg
    18241 aaggtaaaat taatcctgtt acgatagaac ttattggtga aggaagaaag cttgctgacc
    18301 aattaggaaa agagcttgca gttgtgatag ctggatatga agtagagaaa gaagtgaagg
    18361 aattacttca ctatagtgtt gataaagtat attacatcaa tgaccctctt cttaaagatt
    18421 tcactactga tggatatgca atttcaattg caaatttaat cgaaagaaaa aagccagagg
    18481 ttgtattggt tggtgctact tctataggaa gagatatagc gcctagaatt gcaggtaagg
    18541 ttggaacagg tctaactgct gactgtacaa aacttgaaat agattcaaca gataataagt
    18601 tattacaaac aagaccagca tttggtggaa atttaatggc cactattgtt tgtccaaaaa
    18661 atagaccaca gatgtcaaca gttcgtccag gagttatggc aaaagctgta agaaacgaat
    18721 cagagactgg aattttagaa gttgttactc cagaacttac tgaaaaaatg attagaacta
    18781 gattagtaga gatacttcca caagaaaaga aatctgtaaa tctaactgat gctagaataa
    18841 tagtatcagg gggaagagga ttaaaaagag cagaaggatt tgagcttata aaagaattgg
    18901 ctgataaatt gggggcagag attggtgctt caagagcagc agtagattca gggtggatag
    18961 aacattctca tcaagtggga caaacaggaa ctactgttag accagaacta tacattgcat
    19021 gtggaatatc tggagcaata caacatttag caggtatgag tgattctaaa tatatagttg
    19081 ctataaataa agatgcaaaa gcccctattt ttagtatatg tgactatgga atagtaggag
    19141 atttatatga aattatacct gaaatgatag aatcattaaa taggtaactt ttagaaaaca
    19201 ttgaatatgt agaatgataa gtcttataat attgtattgt aagacttatt atttttgctg
    19261 aaggttatag tggggagatg aaacaatgaa agagcagtgg tataaggata tatttgcaag
    19321 agtattatct atgactgatg atggttttat tgttgtaaat acaagtggag taataataga
    19381 tattaatgat aaatattgtg actttttagg aaaagaaaga aaagatataa taggacaaaa
    19441 tatacaaagt attataccaa atacaaaaat gctagatgtt atgaaaaata agtattgtga
    19501 agaaggagct atacatcatt attcaggtgg aaatacaaaa gaaaaaagtg taatagtaag
    19561 taggtcttat gtggaaaatg acaatggaga agtagttgca ggagtagctc aagttaaatt
    19621 tagattgcaa tcatttgatg tagctaaaaa gttaatgtca gaatatatgg agctccaata
    19681 ttataaagag cagtttaagg ataactgtgg ttttgacaag ctcataggtg aaaatagaga
    19741 cttcatagaa cttaagaaaa caggtgtgaa agcttctaaa acaaactttc cagtactttt
    19801 aacgggtgaa acaggcacag gtaaagaagt ttttgctaga gcaattcaca ataatagcag
    19861 taggtcagat aaacctatgg ttagtataaa ttgcgctgca ataccagaag aattattaga
    19921 gtcagagcta tttggctatg acgaaggagc ttttacagga gcaaaaaagg gtggtaaaaa
    19981 aggtaaattt ttagttgcga ataatggtac aatcttttta gacgagatag gtgacatgcc
    20041 tttgaccatg caagcaaaac ttttaagagt tcttcaggaa agtgagatag aaccagtggg
    20101 aggtcttaaa actataaaga tagatgtaag agtaatctct gccacaagaa agaatttaag
    20161 taaaatggtt gaagagggat tatttagaga agacctatac tatagattaa atgtaataaa
    20221 cattcatatg atggaattga aggacaggca agatgatatt ttattgctag caaattatat
    20281 attaaacaaa ttaaatgttg aatacaagga attgaaggtt ttaagtgata aggttaaaaa
    20341 ttgttttatt aactatactt ggccgggaaa tataagagaa ctacaaaatg taataaaaag
    20401 tgcctatgct gtaagtgatg atatggtcat tatgatgtgt gaccttccat ctaagatgga
    20461 taatatcagc agagtagctc aatgtaatgt ggatagcaat tgttcaattc atgaaatggt
    20521 tgagaattat gaaaagagct taattattga tgtgttaagg aagtataatt ggaaatgttc
    20581 taaggcagca gaggttatgg gaatacacaa gagtctttta tataagaaaa taaaaaagta
    20641 tgaaattgaa ttaaataatt aagataactt gaattaaggc aatgctaaaa aagcgccctt
    20701 attcaaagcg aactgtataa actgttttat gcagttaaaa cgtgtaaaag tagatgtgaa
    20761 gtactaatgt caatcattag tactttttta ttatatttat aattactatc tatatttcag
    20821 tatcttttga tatctaagat tattatgttt aatttagtat ataacaaaac cctcttagtt
    20881 atggacagtt ttttatattt taactgtcta ctaagagggg atagtttagt ttaaatttag
    20941 agtaaatttt atctagcatt tataaaaaat attttgaata gcttttttgt tatttagtca
    21001 taattttcta cttctttatc aagttctttg ccacaagaat cacacttata tatagcattg
    21061 tatccgtaat catattcatc aactattttc ttttgtctaa atacaccacc acactcacat
    21121 aggaaaccaa tttttataag tgtaacgata aattgtgatt ttataaattt tatatcattg
    21181 catttatcgt tagtgtaaaa tgtataaata taagagttat cgcactcatc tcttaattca
    21241 aatctttcaa tatatgtgtt attttttaat tcctcaaatt cttctttagt taaatatgat
    21301 ttttgttgca attcatgtaa acgcttaatt tggtccatta tttatatcct cccaataatt
    21361 ttagtatttt aagcttagat aaggaatcta agttttatta aatttatccc aaggaattat
    21421 ttaaatcata tttagtttta ttaattacaa catatataaa ttacacgaaa ataaaactaa
    21481 ttgtaaaaat aatatcctgt gaaaacctaa actactaacc ccttactaac ccgaaaacta
    21541 aaaaaagaaa atatatccaa atataagtga tatataaata aagatgtttc atataaattt
    21601 aactttaaat attctgacaa taatatatat aaatatatta tagttaaaat ataccacata
    21661 ttttgggtat tgtatactgg aattagagta attaatttat tttttaacat acaaatgatt
    21721 ttatgaaaaa aaacaagact caaaaatttt tatattcttt gatagaataa aattgagagt
    21781 aaatttattg aattttataa aatttataag gggtgagtct aagatatgca tgaagataaa
    21841 aatgatatat ttgaatcaat agatactgag gattttgata ataaaacaga agaagaatgt
    21901 ttagagctag taagaaaaaa tggattgaat ttaatataca taaaaaatca aacagaagag
    21961 atatgtctag aggctgttag acaaaattgt aatgcaataa aatgtgttca gaatcagaca
    22021 gaaaaaatat gtatagaagc agttaaacaa gattggagaa tgttagaatt tgtggaagag
    22081 ccaacagaag aaatttgcat gttggctatt aaccaagatg gaacagcatt aaaatatata
    22141 gaaaatcaga cagaggagtt atgcttaaga gccgttgaaa aaaatggtgc agctttggag
    22201 tatgtaaaag aacaaacaga agaaatatgt atagaagcag ttagaaacag cgaatttgga
    22261 ttggcaagag tgaaaaatca aacagaaaaa atatgtatgg aagcagttaa gcattgtagc
    22321 tataatctaa aatgggtaga aaatcaaaca gaagaaatat gtatggaagc agtgagacaa
    22381 aatgggcttg atttgaaatt tgtaaaaaat caaacagaaa caatctgttt aagagcagta
    22441 agacaaaatg gaatggcatt agaatttgta aaggaacaga ctgttggtat atgcttaaag
    22501 gcagtaagac aatatggaat ggcattaaaa tttgtaaagg aacaaacaga agaaatttgt
    22561 acagaagcta taaaacaaga taaaagagca cttagttttg taaaaggaga taaggaaaaa
    22621 tataaggctc tatatgataa taatgaacca tttgcaaaga gatatgttag aaatgtaata
    22681 gaaaaagaaa acagagcatt gattaaaaaa ggagaagaaa atgcaaaaat caaaattgca
    22741 ggaaaacttt atgatagagg catgtctttt gaagatatat cagatatagt tgagattgaa
    22801 ataagcaagt taaaagtgag tttaggtgtt ttttaaagta ataaatgtag gtgtttttta
    22861 tgggaaatag ttaataaaaa aataatcgac tagctctttg taggacagca aaattagtga
    22921 tataatttga tgtataataa attattttta ttagataagg gggaattaaa gttgaagatt
    22981 acagtattat accaaacaag aagtggaaac acagaaaggg tcgctaagct gatagaagag
    23041 ggcgctaaaa aggtggatgg agtagatgta aagcttatga gacttgataa tattgattta
    23101 gattatttaa atgaaagtaa agcagtaata tttggaacac ctacttattt agcaaataca
    23161 acttgggaag taaaaaaatg gtttgatgaa gattcaaaaa aagttaattt agctggaaaa
    23221 ttaggagctg tttttgctac atgtgattat atatgtggag gtccagatgt tgcgatactt
    23281 actatagtag gacatttgat ggttaaaggg atgcttgtgt attctggagg tggttcttta
    23341 ggaaagccat ttatacattt aggacatgta cattcaattg aaggtccaga gttacaagat
    23401 gagaaggcta taatatttgg tgaaagaatt gctactaagg caaaagaact atttgcttaa
    23461 tttctatatg catagtaatg attagagtaa atttagggtt ttaatggaga tttgaagtaa
    23521 aagttataaa ataaaaaatt ttccataaaa ttactcaaga tgaattataa tataattata
    23581 aataaattat attattaaaa tatttaaaat caaacgtcaa tagcgaggga agtttatggt
    23641 atggcatata gaatatgaga ttttttctgc aattatagtt atttttttaa tggtatactt
    23701 ttttagaagt aaatttatac ctactttaca aaacaaaata tattgtgcac ttttaatatt
    23761 ctcattttta tttataattt caaatatttt aggttcattt tgtctaaata atatagacaa
    23821 aattccaatt tttattactt ttttattgaa tcagatatac ttattgttat tgccaatacc
    23881 agcagcactt gtatcatttt atgttatggc tattatttat caagatataa ggtatatgaa
    23941 aaaaagatta ttatttttat ctataccact tataatttgt ataatcttat ctattacaaa
    24001 tccttttacg catattctat ttggattgtc aaaagaaaca ggttatatac gaggttgtgg
    24061 atatgttgca acttttgcat cttttttctt ttatgcaata tattcagcat ttttatcttt
    24121 taaatataga aaagcaatgc atgaatcaaa aatatgggct attagaggtt ttcttatagt
    24181 ttcaattgtg gcaattatct tacaggcaat ttttttacag tatcttctta caggaactgc
    24241 atgtgcatgt agtttacttt tgacatatct ttctatgcag aatcgaggtc ttattattga
    24301 tgaccttact ggtgtactca atagacaatc ttttattcaa gagctagatt tgaatatcaa
    24361 tactgaacaa tcaggattta ttattacaat tgcgcttgat gattttaagt ttatgaatga
    24421 aacttttgga acaaaaaatg gagatattgc actaaaagaa gttggaaaat atttgattca
    24481 gatacatgat gtaaaccatg tatatagaat agggggagat atattttcta ttgttgtcaa
    24541 tagtcaatta ggtatagaac cagatgatat tatcagaaag atagaagaaa gatttaaaaa
    24601 gccttggcta gtagaagaga taagttttaa tttagcaact agtattgctg ttgtttatta
    24661 cccagaaaat gcagaaacga cagaagatgt aattgttgct atagatttct ctatacatga
    24721 agcaaaacgc tctgagtcaa ggcgagttgt ttatgcagat gcgtctataa gtgaaaagat
    24781 aaagaagaaa catgttatta aaaattgttt aaaagaagca ttaaaaaatg atgggtttga
    24841 agtgtactac caacctatat tttctaatag caggggcagg tttacttcag cagaggcatt
    24901 acttaggcta gaacacaaag agttaggttt tatacctcct gatgaattta ttccaattgc
    24961 agaacaaaca ggattgatta actcaattgg attagtagtc tttgagaagg tatgtagatt
    25021 tatagctagt gatgagtttg aagatttaaa attggataat atagctgtga atctttctgt
    25081 agtacaatgt atgcaaaaaa acttagctga tgatttactt tatcttatga ataaatataa
    25141 tgtatcacca actaaattta aacttgaaat tactgagact gtggctatgg gctcttttaa
    25201 tataattaaa gaaacaatgg aaagattaat tgatttaggt gttaagtttg cacttgatga
    25261 ttttggaatt gcttattctg gagttactaa tatgctttct ttaccatttt cagtaattaa
    25321 gttagataag agtttgattt ggtctatgaa tgaagatagt aggcataaac ttacagttga
    25381 gacgattata gcacttataa ataaattgaa tatgaaggct gtagcagaag gtgttgaaac
    25441 cattaagtgt gctgaagatt taatagctat ggagtgtgaa tatttgcaag ggtattattt
    25501 ttctaaacca gttccagaag atgtttttaa aaatttgttg aaagaaaata cttttacaag
    25561 ttaacaccct ttattttttt aattacaact ttgttcattg acactgccag cgatatatta
    25621 taaaattaac acaataataa atattaaatt tattatacat attattaata ttaattaatg
    25681 gagtaaagat ttattatata ttttatttaa ttatcctaaa gtgagaaagg aagatgtgac
    25741 atgaataaaa agaaaatatt agtgccaatt gatgggacag aaagaagtat gcattctttg
    25801 gaatttataa aaggaatatt taaaaaagat gaagttgaag tagaaataat gaatgtaaaa
    25861 gaactagtat ttattgatgg catatccctg gctgaagaga taaaaaattc tgaaaattta
    25921 ggaagaagaa tcttggataa agcagcagag ataatgggag agtatgatgt taaagtacat
    25981 tttacttttg gatatccagg agatgagata attagaaaag caaaagaaga taatgtagat
    26041 tttatagtta tgacaaaatc tactaaaaaa ggtcttacaa gaatgatagg ttcagtaaca
    26101 gcaagtgtag ttaaacaagc aaaatgtata gtgatgattg taccggagta gaattagaaa
    26161 ggtatttgaa atatgaataa aaagatagta gcagtttgtg aaagtgaaag aagtttaaat
    26221 tctcttaaga cagctgcaaa tgatttagga tttgatttag tttatgaaat tcaagaaaat
    26281 gataatataa aaaatgaact ttctatttat gatatagaag atgcatccat agtgttattt
    26341 gttacaagtg attctataga aagcatagag aagattgata gatttataga tagagaattt
    26401 tatgaagttg atccaaagta tataatagag gatgcaaaat ctatagtaag tgaaatttta
    26461 atagacttaa attaatcgtg ggtgtcgcac taagaataga gcgacgcctt tttatatatt
    26521 ttacacaaga aataaagagg ttgtatcaaa atatagataa gagtcttttt cattgttgaa
    26581 atgatgaaaa tatatttgat ttcatacaaa aaagagtacc tcataactaa aaagttcact
    26641 ttgagacact ccatttttat atgttagttg catttaaggt aaaatagaag tttaatcatg
    26701 ttgaaataac tgaattacat cttctaaatg attgtacatt gatgtataac ctaagtaaga
    26761 gtctttacta tcaaatgcaa tcattatctt gttatggtgg ctaatcatat ccttgagaga
    26821 atttctatat ttaaaaccaa agtattggtt agaagaaact gttttaggta ttaactcata
    26881 atattttata aaaattggat tatgagaagc taaaattatc tctttgtgaa attgcaagtc
    26941 agcatcataa cattcttcta tcaaaccatg agaaacctta tccaattcct ctattgactt
    27001 taggtaagta tattttattt tttgaagttc tttagatgta gcatttaaaa ttgctagttt
    27061 agtagcttcg ctttcgataa gttgtcttgc tcttaaagat tctacataaa attcttgtat
    27121 gttatgattt ttatagattt caccatcatc aaataggtca acttttttaa atccttcaac
    27181 tataggactt gaataaggca cagctctaag tattccttcc ttacagagta tatttatggc
    27241 atttcttaca ggtattctac ttatgttaaa taggtcagca atctttctct cacttggtaa
    27301 tttactatta attttaaaag ttccatttag aatgtttaat ttaatataat caataatttt
    27361 ttcagtggca tttaagcttt tcataaaaac tcctaaacat ataatttttt tatgattgta
    27421 ttatatcata gttaagagtt aaatctactt ttttataata tgtataagac aggatagagg
    27481 ccaaaatagc tataatagcc aagataaagt atccatattg tattgaagta tattccgata
    27541 ttttaccaac tatcattgga aatacagaca ttcctatagc ataaattgct tgaaaaaaac
    27601 ccattgcagt agatttctga tgttttggta catttttcat agactcgcta gtaaggtaag
    27661 aaaggagtat acctgtagat atacctggta gtacttgtag tacaaatatt atattaatat
    27721 tttttattgt tggaactaaa aagcaatagg ctgaaactaa aataaaaact aaaggaatcc
    27781 aaaattttgg tccatactta gtacaaaatt tagtagaagc aaagccagaa gaagcaacag
    27841 cacacaccat gtaaaatata gaagatagac ctatgagggt agaactagaa cctaagtttt
    27901 taagaatttg agtagtaaat gacattgttg tagacatttg aataccctgt tgaattaatg
    27961 ccataaagga aaaaaataca agtcttttat ccttgcagat agataaatat tttttaattg
    28021 atattttttc aggtattttt ttagaattat gattttttat atttaaggct agaatcgttc
    28081 caataactga agacaataca ctcatgatac ataaggctgt cattcctaat ttttcataaa
    28141 ataaagtact cataacaaat ccaatcaaca ttcctagatt gtttaccaat attatcgtac
    28201 ttgtagcttt ttgttgatgt tctgtagaaa aaaagttggt atagtgaacc atgtaagaaa
    28261 tccaagttga tgatgatatt ccagataaaa tgttagctat aagaaaacca ttgcctccag
    28321 gagaaaagac tcttataaga gatgctaaac cagcaaagaa aattccagac agtataaata
    28381 ttttatgttt atttatggaa tcagcaaata ctccaacagg aagccttaat aaacattgtg
    28441 atattccata tgcacctatt attataccaa tcatatttgt agatacatta catgcactta
    28501 aatatggatt ttggaaaggt atatatacat attgtgaaaa ccagaaaaat gtaacaatag
    28561 aaagtaaaag taatttttga ttagatattt ttttcatatt atcctccatg tggtattttt
    28621 ataatgcaaa taatatacca tttagatttt ttaatcaagt actttctcta ttaaaaaaaa
    28681 tttaaatata taattattat ttacaaaaaa attatacttt atagttgaat taagaaaacg
    28741 cttatgatac tataaagtta acaaatttga atatataaca gtagacgtgt tactggtaat
    28801 gcaggcaaga tcgaatcaat ttttatagat tatctataga gtactatggg tataatctat
    28861 ttatttggtt cgattttttt gtttaattgg ccataaaaca aaaagcctat agttgaaaat
    28921 ggaggatttt ttatggatta taaaaatata gctcaagaaa ttctactaaa tgtaggagga
    28981 aaagagaatg taaatgaagt gactcattgt atgacaagac ttagatttaa agtcaaaagc
    29041 gcttctaaag tgaataagga taaattatct aaaactgaag gagtaataac tgttgttgag
    29101 agtatgggtc aaatacaagt tgtaattgga aataaagtaa aaaaagtata tgatgaggtc
    29161 ataaaaattg tacctcaatc aaataacata ggtaaaaaga atgaaagtca agaaaagcaa
    29221 ggtataatta attctatatt atcagcagta gctggtatat ttacacctac aatacctgct
    29281 atagcagggg ttggtatgat taaaggtatt ttatcagttc ttgctatgta ttatatgaat
    29341 aaaaatggag tagatataaa agaaacgcaa agctatatta tcttaaatgc tatggctgat
    29401 tctatattct attttatgcc tattatacta ggatatactg cggctaaagt atttaatgca
    29461 aataaaataa tatctatggt attaggagct acactttgtt atccaacttt tactgctttg
    29521 atggcaggtg aagaatctgt tagattttta ggattggcag taacaaaagc tacgtataca
    29581 tcatctgtta ttccaataat tattgctata tgggctcttt catacgttga aaaagtatta
    29641 gaaaagtata tacctgaggt aattaaaata ataatggttc caactttatc attggtagta
    29701 atgttacctg caacattatt tttatttgga ccaatcggaa tttatatagg aaatgtcata
    29761 aacttttctt ataagtatat ttatgaatta agcccagcat tgtgtggagc atttgtaggt
    29821 gggttatggt gtgtgttagt tatatttggt gctcatagag cattattacc aataggtata
    29881 agtgatgttg ctcaaacagg aagacaaaat ttattggcgt ttgcaggagc tgcaaacttt
    29941 tcacaagcag gggctgctct aggagtattc tttaaaacta aaaatcaagg actaaaaaca
    30001 atttctatgt ctgcaactat aacagcttta tttggtataa ctgaacctgc aatatacggt
    30061 gccaatctta gattaaaaaa acctatggta tgtgccgtaa tatgtggtgc atttggaggt
    30121 gcgataatgg gaatgggtgg agcttatggt aatgcatttg caaaccaagg tgttttaact
    30181 atacctgtat atgctgaagc aggtgcatta ggtttcttaa gttatctagg tggttgtgct
    30241 atagcatttt ttggttcagc tatttcaact tatctagttg gatttgaaga tttggaagag
    30301 tcttctaata aggaaaattc atctataaaa gtagaaacga aggatggtgt tattgatata
    30361 acttcacctg tagagggcga atgtatagaa ttgtcagaag taaaggatga tgtatttgct
    30421 tcaaaagcta tgggagaagg tatagctgtg ttacctacaa aaggagtaat aacagcccct
    30481 actgattgtg aagtagcttc tttatttcca acattacatg caataggatt aaaattagat
    30541 aatggagcag aaatgttaat acatgttgga atcaatacag tagagttaaa tggtaaatac
    30601 tttacaaaac atgttaatca aggagattta gtgaaaaaag gtgataaact tatatccttt
    30661 gatatagata aaataaaaaa ggctggttat gatgtaacta cgccagttat agtaaacaat
    30721 acatttgatt aaaaaatagc tacgcacact cgtgacttta gtcatgagtt agtagctaaa
    30781 atagtcagca tatagggaaa cctatatgta gagataggat agaacctatc caatactact
    30841 tgaattgctg gaaaccccta aagctaacca aactaaaaca taaggatgaa ataaacctaa
    30901 gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt ataaggttaa atcctaagta
    30961 ctttaaaatg ggcaatcagc aggtaaactc cgaataggag aaacttcaac gactattcct
    31021 cttgagggaa gtacactaca agttatcggt agtggaagtg ggtagacctt aacggataat
    31081 gccgaaggat aagatatagt ctgtgctt
//
LOCUS       DAELNY010000026        30915 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.026, whole genome shotgun sequence.
ACCESSION   DAELNY010000026 DAELNY010000000
VERSION     DAELNY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.026"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..780)
                     /locus_tag="KPE55_001057"
     CDS             complement(<1..780)
                     /locus_tag="KPE55_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0492153.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCF
                     NKFKESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNL"
     gene            complement(819..1679)
                     /locus_tag="KPE55_001058"
     CDS             complement(819..1679)
                     /locus_tag="KPE55_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0492154.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            1948..2157
                     /locus_tag="KPE55_001059"
     CDS             1948..2157
                     /locus_tag="KPE55_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0492155.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2157..2423
                     /locus_tag="KPE55_001060"
     CDS             2157..2423
                     /locus_tag="KPE55_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0492156.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2452..3024
                     /locus_tag="KPE55_001061"
     CDS             2452..3024
                     /locus_tag="KPE55_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0492157.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3259..4449)
                     /locus_tag="KPE55_001062"
     CDS             complement(3259..4449)
                     /locus_tag="KPE55_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0492158.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENSENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4709..5140)
                     /gene="dut"
                     /locus_tag="KPE55_001063"
     CDS             complement(4709..5140)
                     /gene="dut"
                     /locus_tag="KPE55_001063"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG0492159.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5253..7481
                     /locus_tag="KPE55_001064"
     CDS             5253..7481
                     /locus_tag="KPE55_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG0492160.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7592..8077)
                     /locus_tag="KPE55_001065"
     CDS             complement(7592..8077)
                     /locus_tag="KPE55_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0492161.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8329..9552
                     /locus_tag="KPE55_001066"
     CDS             8329..9552
                     /locus_tag="KPE55_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0492162.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9556..12690
                     /locus_tag="KPE55_001067"
     CDS             9556..12690
                     /locus_tag="KPE55_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG0492163.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12724..14022
                     /locus_tag="KPE55_001068"
     CDS             12724..14022
                     /locus_tag="KPE55_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG0492164.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14311..14556)
                     /locus_tag="KPE55_001069"
     CDS             complement(14311..14556)
                     /locus_tag="KPE55_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0492165.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14770..17397)
                     /locus_tag="KPE55_001070"
     CDS             complement(14770..17397)
                     /locus_tag="KPE55_001070"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0492166.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17458..18300)
                     /locus_tag="KPE55_001071"
     CDS             complement(17458..18300)
                     /locus_tag="KPE55_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG0492167.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18307..18945)
                     /locus_tag="KPE55_001072"
     CDS             complement(18307..18945)
                     /locus_tag="KPE55_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492168.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19381..20673)
                     /locus_tag="KPE55_001073"
     CDS             complement(19381..20673)
                     /locus_tag="KPE55_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0492169.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(20710..21066)
                     /locus_tag="KPE55_001074"
     CDS             complement(20710..21066)
                     /locus_tag="KPE55_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0492170.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21101..21460)
                     /locus_tag="KPE55_001075"
     CDS             complement(21101..21460)
                     /locus_tag="KPE55_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492171.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21529..23502)
                     /locus_tag="KPE55_001076"
     CDS             complement(21529..23502)
                     /locus_tag="KPE55_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0492172.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKCQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDLSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNCILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(23724..24737)
                     /locus_tag="KPE55_001077"
     CDS             complement(23724..24737)
                     /locus_tag="KPE55_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0492173.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(24750..25295)
                     /locus_tag="KPE55_001078"
     CDS             complement(24750..25295)
                     /locus_tag="KPE55_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0492174.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            complement(25671..26540)
                     /locus_tag="KPE55_001079"
     CDS             complement(25671..26540)
                     /locus_tag="KPE55_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0492175.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWLLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            complement(26692..27807)
                     /locus_tag="KPE55_001080"
     CDS             complement(26692..27807)
                     /locus_tag="KPE55_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG0492176.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            complement(28043..29179)
                     /locus_tag="KPE55_001081"
     CDS             complement(28043..29179)
                     /locus_tag="KPE55_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0492177.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            complement(29235..30698)
                     /locus_tag="KPE55_001082"
     CDS             complement(29235..30698)
                     /locus_tag="KPE55_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0492178.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHTQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
BASE COUNT        10495 a         5245 c         3815 g        11360 t
ORIGIN      
        1 taaattttgt atatttacat ctatgattgt taataagtac attctcacag tttttattat
       61 ttcaacagaa aaatctacaa tattataatt tccttctttt ttatataatg actccttaaa
      121 tttgttaaaa caattatcta aattagaatt tatatcgtta aatggtggtc ttattatgtc
      181 acgtattatt aaaagatttt gtatatctac attatttgtg attgcctcat ccaaaataaa
      241 taaattatat gcatcattta atgggtcatg ggcatctcca ctaaactcta agttaacaaa
      301 ttcaagtaaa tttttcaatg atatatagtt catacatctc ataccacaat ttatatattt
      361 ttcttttata tctacaaata aatctctaat gttatttaca aaccttggat ggttattttt
      421 tttagcactt ctcatatctg tgttattgaa acatgtcaaa tccaaattac caaaagtata
      481 tattccttta atatcagaaa atatgccaag ccatttttta aactttctca tgacctcttc
      541 ataacttggt gcactcttca attcttcaga agatatatgt gttaattctt gtatatgagg
      601 atacaactcc tcattactta ctggtcttat taaagattta aatttatcaa tattttttgt
      661 tttttcatca tacataactg ctccgattgc aattatatca gaattaaata ctgctctttt
      721 tgatttagaa tttggcatat tcatttcaaa atctatatat actcttttca atataaacac
      781 cctttccaat tgtttatagt gattcttttt aactattttt attcaaaatt ttctaagaaa
      841 gaatgtttct ccccatcttt ttgccaacca agtactgaat caagatttac attttctgcc
      901 gccttaaata tagatttttc tacattttta aaatttgatt ctaagctctt tattaagtct
      961 tttatctcat atcttttatt acctgttttt ttagcagcaa ccatacatgc acaatttaat
     1021 ggccatatac cactattttg aataaatctt ataatatgtt cttctcttat ataataaagt
     1081 ggtcttataa tttttatacc ttcaaaatta gtagaattaa gtttaggaag cattgtttta
     1141 aaatttccag cacacaataa attaagcatt gttgtctcta ttacatcgtc ataatgatga
     1201 cctaatgcaa gtttgttaca ccctaattct tctgcctttg aatataatgc tcctctcctc
     1261 attcttgcac acatatagca tggataatct tttgctattt catctgctat ctcaaatatc
     1321 cttgaatcaa ataaatgtat tggtatattt agatactcac aattatctat aagtaattgt
     1381 cttatatttg catgatatcc agggtccata gcaataaatt caacatcaaa attaacttgt
     1441 ccatgttttt ttaactcttg aaacatttta gccataagaa tactgtcttt tccaccagaa
     1501 atcgctacag ctatcttatc accttcttca accaatttat aatctcttat tgccttcata
     1561 aacttagacc ataaattttt cctatatttt tttattatac ttttttctat atctcttact
     1621 ggaagtcttt cctcaaaagg aactataatt tcacagcctt ttcctgatag ttcactcatc
     1681 taatcacctc gtatatttat gttaataata ttttacaaaa aagtctacct tcatccacaa
     1741 aatactataa tttttacttt aattttacta aataatttat agtttacaat aacacataaa
     1801 cttataataa cataaataac ctctttcttt tattatcttt tttatttttc tcatttaata
     1861 ttttattatt agtaacgatt tttaaatgat tgaatattat acactagata gagttaccta
     1921 tctatttaca acaaggaggt tttcatcttg gaaaataaga ctagagatat caaaaaaaat
     1981 tgtggtccat ctttggctag accatacatt aataatcaaa ttttcacaga aatgtactgc
     2041 ctttctgatg cattaaaatt tggaactata tttccagaac taaacttact agagtctgaa
     2101 atgtacaaca aagaattata tgctaagcca aaaaaatata gaggaggtag aagataatga
     2161 aagactcatc tagagctgaa cttatgagaa aagttcagga aacatctttt gcatgtgttg
     2221 atatgaactt atatttagac aatcaccctg atgataaaaa tgctatcaac acttataatt
     2281 cattatgtaa tcaatttgcg caagctagat atgcttacga aaataaatac ggtcctttaa
     2341 ctaactttgg gtatgctcca agtagatgcc cttggcaatg ggctgaccaa ccttggcctt
     2401 gggacagaga atttaattat tagacatcaa gtattggagg attgaaaatt catgtggata
     2461 tatcagaaaa ctatacaaca cccagttaat ataaaaactt gtgaccctag aatggctaaa
     2521 tttcttataa ctcaatttgg tgggccaaat ggagaacttg ctgcatcttt aagatattta
     2581 agccaaagat atacaatgcc tactggtaat atgcgtgcac ttttaacaga tattggtaca
     2641 gaagaactag ctcacgttga gcttatatgt actatggttt atcagttaac ttctgatgca
     2701 agcccagaag agttaaaagc tgcaggtctt ggttcaaact atgctcaaaa tggatatgga
     2761 atttatccaa cagattcaaa tggtgttcca tttgatgtaa gacctatagc agttatgtca
     2821 aatcccgtaa ccgatttaca tgaggatatg gcagctgaac aaaaagcact tgcaacttat
     2881 tatcaactta taaacctaac agatgacgtt gatgttatag atgtattaaa attcttgggt
     2941 caaagagaaa taattcatta tcaaagattt ggtgaagcct taatggatgc ttacgagtta
     3001 gaagaatctc aaaaaatgtt ctaaaatttt tatttgtata actattatta tttgataatt
     3061 tataatttac tgttgtgtta aaatttataa aaaatataaa tataatttat tttctataaa
     3121 cagagaaaat aaataaaaag caggtatcaa tcaaatagta acatgtatta tactatacat
     3181 tactatcaat attgtacctg ctttcatatt ataagcaata aagaaaaatc tatttattag
     3241 gttttataat aaaaacaatt ataaaactct tcttgcattt acatatctac ttgaatagta
     3301 gctactattt atattagata ttgttacttt ttttgatgaa cctgaagatg catgtatcat
     3361 atctccattt cctatgtata ttccaacatg acttacagaa ccattatttg aaccttgtgt
     3421 atcaaagaat actaaatctc cacttctaag gtctcctcta tttacatatg ttccatactt
     3481 actttggtct ctagatactc ttggtatact aactcctaca gattttttca taacgtactg
     3541 cgtgaatcca gaacagtcaa aactatttgg accttctgct ccccatacat atggttttcc
     3601 aagtagtgtc tttgcaaaac ttattacact atcagctcct tgacttggag aattagtcga
     3661 ataagatgga attgaagtat caacagaacc tttttcagca aggtattgac ttgacacata
     3721 tccagtttga ttgccataac ttatttttgt ccatcctcca ctttcagaaa tacttccaac
     3781 ctcagtacca taacttaatg tagctatttt agaatgactt gtacctggtc ctgttctaac
     3841 gttcaatgac tttgctgtta caacctttgt accctttaca aggtcatttg aactgttttc
     3901 agaattttca tttgtagagt catttacact agttcctaaa tatttacttg aaacataacc
     3961 tactctacca ttagaactta tttttgacca tccatttgac tcagatatgt atccgacttc
     4021 agtcccatat cctagtgttg atattttaga actacttgta cttggtcctg ttctaaaatt
     4081 taatcctttt gctgttacaa cttttgtcga cttaactgaa gtatcactac tttcattgct
     4141 acttccaagg ctatttattg ctacataatt tccatgcaca taacctgtgt ttccattata
     4201 tttaactttt acccaagaac cttcttttcc tacgtactca actttatcac ctttgtaaag
     4261 tttccccatt gattcatggt ttgtacctgg tccttttcta aaatttaccg cattccctgt
     4321 tattgttctg ctttctatac tcacactctc tacttcagac tcattagcat atgctaaatt
     4381 ccccatagta ggcattatta tcgcagttgc cattatactg gctgctattc tcttttgatt
     4441 aaaattcata actttccact catctcctct tttctactat ctttatttca cttcaaaata
     4501 taaaagaaat aattgacact atcaaaatat caattatttc ttttatatta atattattac
     4561 aaatttgtaa ctatttgtct atagtaatat ttgcttacca taaattacca tgtataaatt
     4621 ttaatcttta atagcctgta atttagtaat atagccatct tttacctttt ttaaaaatgg
     4681 ctatattttt taactttttt gtaacttttt aaaatccagt tgagccaaac ccaccttttc
     4741 ctctttcgga gtcacttaat tcttttactt cttctatgtt aatatcatag ataggtttta
     4801 ctatcatttg agcaattttc atatgctttt caaccttaaa atcattttta ccatgattta
     4861 ttaatataat tttaagctct cctctgtatc cttcgtcaat agttcctggt gtatttaata
     4921 cagtaacaga atgctttaag gcaagcccac ttctaggtct tacctgcgct tctgtcatag
     4981 taggaagctc tatacatatc ccagttttga taagtttagt ctctccagga ggtattacaa
     5041 cctcttctat agaatacaaa tccataccag catcaccttt gtgagcaaaa ttaggtatta
     5101 tagcatcatc atttaatttt tttactttta aattgtacat cttatatctc ctttcaactt
     5161 tctataaata ttataactta aatttcgacc tcttgaagtt ttttattttt ttgtggtaat
     5221 ataattatta gtgttttaaa ggaggatttt ttatgaaact tgtacttgct gagaaaccat
     5281 ctgtggcaaa aactatagca tcatttttag gtgcaaagac cagacaagat ggttactttg
     5341 aaggaaatga ttatataatt acttatgcag ttggacactt agtatcatta tatgatatga
     5401 aggattatga taaagataaa tactctggct cttggaaaat gaacaatttc ccatttgttc
     5461 ctgaggataa gtttaaattt aagattgata gttctaaaac taaacaattc aacactgtaa
     5521 aaaagctttt gaatagaaaa gatgtagaat atgtaataaa tgccactgat aatgaccgag
     5581 aaggagaact tatagcattt ttaatatttt tactggcaaa aaataaaaaa ccagtaaaaa
     5641 gaatcttggt aaatgaatgg acaccagaag atattacaag aggtataaaa aacctaaaag
     5701 atgaagatga aatgagaaat cttcaagctg ctggatatac taggcttatt actgactggt
     5761 tgattggaat aaatttcact tctgttgcta cattgaaata cggaaatggg aaactattaa
     5821 acataggtag ggtaattctt ccaacagtta aattagtcta tgatagagat atggaaatac
     5881 taaactttgt tcctaaaact tattatgaaa tagaagggca ttttaaagct gaagctggtg
     5941 aatataaagg taaatatgtt aagggtaaag aaagtaaatt tgatacactt gaagatgcaa
     6001 ataaaattat agcttcaatt acatctgaaa ctggtaaaat actagataaa aaagttacta
     6061 tgtcaaagga atatgcacca aaacttttta gtttgacatc acttcaagga tatataactt
     6121 ctaagtattc caattttaca tctgataagg ttttaaatgt ctgtcaatct ctctatgagg
     6181 gtaatggtaa gggtggatac ataacttacc ctagaactga ttccattttc ttagaagaaa
     6241 gtttggcttc aaaagcttct caaactttag ataaattaaa ggttggtctt gaatatgaaa
     6301 ataaaattaa attttctaaa actaaaagag tttttgattc ttctaaagta gactcccata
     6361 gtgctataat tccaacttat ataattccaa aaagtttatc taaggatgaa caattagttt
     6421 atgatgccat aaaagataga tttgtagcaa actttatgcc tcctgctgaa tatgagaata
     6481 cagaaattaa gactgaagta gataattgta ctttcttaac aaaaggtaag gtgcttaaat
     6541 caaaaggata ccttgaggta tataataagg aagaaaaaaa cgatttactt ccacttgtaa
     6601 ataaagatga tgttgtagat gtgttagaaa taaaaccatt aactaagcaa actactccac
     6661 ctaaaccgta tactgaggac acacttttaa aggctatgaa aaattgtggt aaaaatgtgc
     6721 ctgaagaaga tacaacagtg ctatctggat attcgattgg aacatcagcc acacgtgctg
     6781 atgtgttaaa gaagataagt caagtgggct atgtaaagaa aaaaggtaaa tcttattcta
     6841 taacagacct tgggaaaaat ttagttgaaa tttttcctgt taaagactta tttgatgtag
     6901 attatactgg aaaacttgaa aaaagtttaa gcgatattca aaaaggaaag tatacaagaa
     6961 aagaatatct tactaatatc attagtttta tatatcaaaa tgtcaatatt ataaaacaag
     7021 atgcttcaaa aaatataaac acagaagcat atactttcga ttcaaaaact aaaaaattta
     7081 ctaaaacaaa agaaaaaaaa cagaaaaaag atgtaactaa tactgaaaaa gaagttgcta
     7141 tatcaaagga ttctaataac aaaaaagaag ttaaaaatgg tgatgaaaat caatctcttg
     7201 gcaaatgccc catttgtcaa ggtgatgtac tagaatttga caaaggcttt gcttgtaaaa
     7261 atcataaaga atgtaaattt gttatttgga aaaatgataa gtatttaagt ctttataaaa
     7321 agaaagttaa caaaacaatg gtcaaaaata ttttaaaaaa aggtgaaact aaagtaaaat
     7381 ctttaactgc taaaaacggt aataaatttg atgctacatt aaaatatgat aaaaatccag
     7441 atactggcta tttcaattgg aaaatagagt ttgacaacta aatattcata agaaaagaaa
     7501 tagcagtagt ataataatct atattatatt actgctattt cttttcttat gaagtttcaa
     7561 cttttttaat agtgactttt acaatcacac tctaacctaa tttctttact atttctgata
     7621 actttaatgt atgttcatct aataaagtta gttcctcttc agaaagatta gataattttt
     7681 ttactagaga ttctttgaga atagctttta ctgcatcaaa taatttaaat gctttatctg
     7741 ttaattcaac cctaagaatc cttctatctt ttgggtcagg aaatttattg actaaaccat
     7801 gttccactaa tctgtcaata atcggagtca tatttgattt taaaattcct aaattagaag
     7861 ctatttgaga aattgatgat gaatttgatg tcataagata aagaattacc ttagcatgtg
     7921 atggagccat tggacattct tcaaaataag ctttcattat atcagagtta ttttgaaact
     7981 tttttaacaa atcgctttct ttaaatatat tattttcaag cacaaataaa aaatctaaaa
     8041 aagagtccgc tattttaagt atattgttgt tatccatatt tgtaacatcc cttcaataga
     8101 taatataata aaacattgtt gtttaatata gtataaatta taaactatta ataaatttaa
     8161 aacaagaaaa agttattgac tcataataat tataaattta taatcaaatt agagaattat
     8221 aaattgccaa taaagggaag ttttttaatt agtctaaaac taaacttaaa ttataagaca
     8281 ctttaaattc tacaacaagc tagtgtgcaa tatataatag gaggagatat gaaaagatat
     8341 ttaatagttt tcttaatgtg tagttcactg tttttagttg gatgtggaaa aacagaagaa
     8401 tctgaacctg agaaaccaat agcagtttct gttcaaaaag caataggtgg agaaattgaa
     8461 aacacaaatt ctttctcagg aactacaaag gttaaagatg aaacagctgt tactgcacag
     8521 actgttggca cagttcaaga agtgtatgta aaacttgggc aaaatgtaag aaaaggagat
     8581 gaattattga gtataagcag tccagagcta gaaaatagtg taaagcaaag taaagcctca
     8641 ctagatttag caaaagcaag ttattcaagt gccactggtg gttcattaga ggcacaagta
     8701 aatcaggcaa aaacagctct tgacaatgca aagattcaat atgaggagtc tcaaagaaat
     8761 tatgacaata ataagattct ttatgaacag gaagtcatta gtttagacca atttaaaaaa
     8821 atagaatttt ctttagagca aacaaaacaa cagttagatt ctgctcaaag agcatacgat
     8881 acagcaactt caaaaagtat acctcaagct aaagcacttg ctaaaaaaca gcttgaccaa
     8941 gctcaagtat catataattt agcaatgagt aatttagaca aattaacatt aacatctcca
     9001 gtagatggta caataacagc taaaaacttt gattcaaagg aaatgataac acaatcacaa
     9061 ccagctttta ttatttctaa tcctaatata ctagaagttg atttaaatgt agcagaatca
     9121 gatataggta aatttaagaa agatggaaat gtagatgtta taattgaaga ccaaaggatt
     9181 ttaggaaaaa tagattatgt accttcagta gttgaccctc agacatcttt atatcctgtt
     9241 aaagtacttg taaataatac aaacaacaaa tttaaggcag gtatgtctgc tcaagtaaac
     9301 ttaagtgttg aaaaagaaaa tggtgctgtt acagtaccta aaaaagcaat atttgaagaa
     9361 aatggtaaaa aatatgtata catagcaaca aaagacaata tagcaaagaa acacttggtt
     9421 caaactggaa ttgtaacaga ggataaaata gaaattaaaa gtggtgtaag tgataaagat
     9481 acagtgatta tcggagggat aagtcttatt tctgacggaa ctaagatatt tcctgtagaa
     9541 aaggagaagt agactatgaa tttaacacag acttccgtta aacgtcctct cacaatcata
     9601 atggtctttt tagtattaat agtttttggt ggtattggat ataaaaaaat gtcaataaat
     9661 ctaatgcctg atatagaaat accagttgta atggtcatga ctacttggac aggtgctggt
     9721 cctcaagacg ttgatgaaca agttagtcaa aaagttgatg aaagtttatc tgccgtttca
     9781 aatgtaaaat ctactatatc aagttcacaa gaaagtgttt ctatggtagt tgctcagttt
     9841 gaatttggaa ctaatttgga tgaaataatg aatgatgtta ggtctaaagt tgatgcatta
     9901 cagaccagcc tcccagatga tgcagctaaa ccaacagttt taaagctgga tatgaatgca
     9961 caggctatag gtcaacttgt catatctgga ggaaatgaaa atagttcaca agccttaaga
    10021 aaatacgctg aagatgtaat tcagcctaaa atagaatcca tagatggtgt tacttcagca
    10081 gaccttaaag gtggagaaaa agcacaggtt aatgtaatag ctgacccagc tgttctttca
    10141 aattatggag ttagtttatc cactataaaa ggtgtcttat cctctagtaa taaaacattc
    10201 ccttatggtt caattactca aggtgaggat aaaatagttt taagagcgat tgataaatta
    10261 gaatccctag atgatataaa acagattcaa attcctgtaa aaggaggaaa tacagttaga
    10321 ttagataatc tttgtagtgt agattatgga tatgcagaca aagatagtat ttatagatac
    10381 aatggaaaag acagtttggt aatagatatt tctaaacaac aagatgccaa cactgtaaaa
    10441 gttatgcaga gtgcatacaa atacgtagat gaattgaata aggaaaatcc tcaatttaaa
    10501 attaaaattg ctaatgatac aagttcatac ataagtgaat caataaacag tgtaatgagc
    10561 aacttgctta taagtgctgt tatcgcattt attgtaatat ttgctttcct aaaaagtgta
    10621 agagcttcac tagttgttgc agttgcaata ccaatttcta taataggtgc tatagcaatt
    10681 ctctacttta ctggtgaaag tttaaatctt attactgcaa gttcattggt aatttcagtt
    10741 ggtatggttg ttgataatgc tacagttgta attgaaaata tatttaaata taggaaaagt
    10801 ggcagattga gtcttgatga ttgtgctatt gagggaacaa gaactgtaac aaatgcaata
    10861 cttgcatcaa cattgaccac aatagcaata ttcttaccta tcatgtttac agaaggtata
    10921 gctaaaataa cttttggggc attaggtaaa acactaatag cttcactagc attctccttt
    10981 ataggtgcaa ttacactcgt tcctagtgta tttgcaaaat taagcagagg aaaaaatagt
    11041 caaaagatgc aagaaaaaga tagtcctata tttgataagg taagtgaagc atataaaaaa
    11101 ttattaagtg tatgtttgag acatcaacgc ttagttgtaa tatcaagttt acttattttt
    11161 gtagcttcct tatttggaat gggatttata ggaatggact ttatgcctgc agcagacaag
    11221 ggtgaattat ctatttctat agaacttcca aaaggtctta gcctagaatc caatgattac
    11281 tatgtatcta tgactgagaa aaaagtctca gatattccag aaattaaaac tttaataaca
    11341 actttgacat cagattcaaa taatagtaaa gcaagtatag caattgaatt agaatctcaa
    11401 aaaaatagaa aaagaactac tgatgaaata gaaaaagaga taattgatag agtggctact
    11461 gtaccagatt gtaaaataaa tgtaagtcaa aacagtagtg ttatgggtgg agctggagga
    11521 gcagacttca cattagaaat gaaaggtcct gatttagatg tattaaaaat actgggaaaa
    11581 gaagctgaaa aagaatttag ctctataaaa ggatttagaa atgttgaaac ttctcttaca
    11641 gatacaacac aagaagctca atttactata gataaacata aggctcaaga atatggtgta
    11701 aatactgctg aaattgcagg catgttacgt acagcaataa atggtgactc tgtaaccact
    11761 gctactattg atgattacaa agtggatgta aacctaaaat ttaaagcaga tactattgat
    11821 agcttagaag atataaagca actaaaagta aactctgcaa ctggaaaaga agttcctctt
    11881 ggtgcttttt cagatataaa aatggcagat ggtctacaat ctatatctag gactgatgga
    11941 gatttttctg taaatataac tgctacactt gacaatttag atgctggtac tgctacaaaa
    12001 ctagcactag aaaaaacgaa taaaatggta attcctaaag attactctat aggtgttggt
    12061 ggtgaaacag aaatgatgaa tgaatctatg gcaagtcttg gattttcaat ggtcatagct
    12121 atagcattag tttatatggt aatggtagct cagtttgaat catttagtaa accatttata
    12181 atcatgttct gtgttccatt tgcattcgtt ggagttgtgc tttcgcttct agttagtggt
    12241 caaagtttaa gtactgtcgg tatgctaggt ataatcttac ttatcggtat tgttgttaat
    12301 aatggtatag ttcttattga ctacatagag caacttagaa aaacaaatcg taacaatgat
    12361 ttaattggtt tggtttcaat tggttgtgca gcaagattaa gaccagtctt aatgactaca
    12421 gcaacaacaa ttttaggtat gttgccaaca gcactatctc ttggtgaagg tggagaaact
    12481 atgcaaccac tagccatagt tataatgggt ggtctaactg tatctacact tataactttg
    12541 gtgcttatac ctactatata cttaatattt gataaatggg aaaataagtt ttcagacaaa
    12601 atgcctaaaa tatttggtaa gataacagat aaaataggaa gtataaaacg taaaataata
    12661 aagaaaccaa aattgaaagc taaagtttaa atagaatgga gggtgattgt attgaataaa
    12721 aaaatgagca aaatagttgc aattggctgt ggtgttggta taataactgc caatttaatc
    12781 ccagtttacg cagataataa tacttcttct ataaacaaag ttgataattc tattttaagt
    12841 gactcaaata aaagaacatc tgaaaaggaa attttgactg ctagagaagc tgttagtgct
    12901 gcacttaaaa atagtgaaaa attaaaaatg aaatcagaag aaataaaaat gttaaaagaa
    12961 aagttagaag tacaagatga gtttgatagt tttactggaa gtgacaactc gtttccatac
    13021 gaccaaattg gacttcttaa aaatcaaagt gaacaggcta aaggctttat ggaggaccaa
    13081 atagcaaatg atattactaa taaatttaac gatttagttt caagagaaaa tgagctagat
    13141 aagataaaaa acaatcttga aataaaaact aaagaaatta aagatatgaa attaaaaaaa
    13201 gatttagggt tagtaacatc tttagaaact gaatcagctg aacttgaact tcaaacacta
    13261 caaaatactc aaaaagctaa acttcaagaa ctaaaaaaca atcaagacta ttttaagtta
    13321 ttgactaata tagatttaaa taattatcaa ttggataaag aatatagatt tgaatctttt
    13381 agagtaggtg gctcagttga ttcatatatg gaaggtaagg ttaatgaata tttaaaatac
    13441 gaccaattga ttttagaacg tacagaagaa agctttaatg ataaagatga gaacaaagca
    13501 gatttaccag atagacctaa ccttacaaga ccagttgctc ctacaaaacc agagcaaggt
    13561 aacctaactg atgatgaata caaggttctt atggataagt atgaaaaaga ttatgataga
    13621 tataaaaaag aagttgaagc atacaatctt gaaagacaga cttatgctgc tggtcttact
    13681 acttatgcaa attatcttca acaaaaattt aatactgaaa atggtctagt aacattagaa
    13741 gacagtaaaa aagctcttaa aaaagggtta attgatagct atgctcaatt acttgcttta
    13801 gaagatacaa ttcagattac taaaaaacaa cttgatttat cagaaaaaca attaaaaaat
    13861 actaaattaa gatatgattt aggattaatt actctaactg attataaaaa acaagtggta
    13921 agtaatgaag atgctaaaaa tagctatgac tctcttattg tgaattataa ctcactaaaa
    13981 aatggtatag aaaaaccttg gatacttaat actggaaaat aattctggta aatagaaaga
    14041 ttactttaac atacgtttga ttaaaaaata gttatgcaca ctcgtggctt tagtcatgga
    14101 atattcagaa ggcaaataat agaaatagat tagtaaaaat atatttactt ataaaactgt
    14161 atacttttat agaggctatc tcaaaataga gataagcctc ttttttattg tttaaattat
    14221 aaaaatatat ttgattccat acaaaaagag tgccttatga actaaaaagt tcattttgag
    14281 acaccctcaa aagcttaact ctaaatttaa ttatttttta tcaaatgtct catctatttt
    14341 acatctcacc atgttaacaa attcagaaat attatatcct ctaagtttgc atccacattt
    14401 aggacaataa tccagtttcc tagataccaa agccctacaa ctaggttctg gacatcttat
    14461 ttttagactc tcatctgcta ctttaaatcc acatcgtggg catatactac ctggacgtga
    14521 tgatacttcc tttccacatt taggacactt tgtcaacttt atcccctcca gtaaactcaa
    14581 tttaattgat agttgaaatt tctctctaca ataatatatg caatataatt aaatacgttt
    14641 cacttacaac tatataagtt aaatttttat aataaaaatc ccctatattc aatttacaaa
    14701 tacttaattt gatacattga acaatagggg attttatttt tatttagtta catattttgc
    14761 agtctattat tatctaggat tttttataga tgcttgagct gctgcaagtc tagcaattgg
    14821 cactctaaat ggtgaacaag atacatagtt aagtcctaca ctataacaga actctactga
    14881 agatggttct cctccatgct ctccacatat tcctagtttt aattctggtt taacttctct
    14941 acctaattta gctcccatct taactaactt accaacacca ttttggtcta gtacttggaa
    15001 tgggtctttt tctaatattt cttcatctac atattgacct aagaatttac cagcatcatc
    15061 tcttgaatat ccaaatgcca tttgagttaa gtcattagta ccaaaactga agaagtctgc
    15121 ttcttgtgct atttcatcag cagttaaaca agctctaggt atttctatca tagtacctac
    15181 agtataagga acttctatac cttcttcttt tattatagcg tcaacagttt ctactatcat
    15241 ttttcttatt atttttaact catttacttc tccaactaat ggaaccatta tctctggaga
    15301 aactttaaca cctgctttga ttgcttctat agcaccttgt attatagctt tagattgcat
    15361 tacacatatt tcaggataag ttattgcaag tctacaacct ctgtgtccaa gcattgggtt
    15421 aaactcatct aaatcaacta ttctcttctt aatatcactt acttttattc ccatagattt
    15481 tgaaagttct tctatagtct catcatcatg aggcaaaaat tcatgtagtg gtgggtctag
    15541 taatcttata ttaactggtt ttccatccat aactttaaat aactctacaa aatcctctct
    15601 ttgcattggt aatatttttt caagagcttt ttctctttgt tctactgtat tagataatat
    15661 catttctcta actgctggca atctagcttc atcaaagaac atatgctcag ttctacatag
    15721 acctatacct tctgcaccaa actctatagc tgcacgagca tctctaggat tatctgcatt
    15781 agttctaacc atcatacatt tgtgtttgtc tacccagttc attaattttt caaaatttcc
    15841 agttaaagat acttctgttt tcttaacatc tcctaggtac acatttcctg tagaaccatc
    15901 tatagaaatg tattctcctt cttttataac tacatcatca agtgctctta tttctttatt
    15961 aaactcatca acttttattt ctccacatcc agcaacacaa catttaccca tacctctagc
    16021 aacaactgct gcatgtgatg tcattccacc tctagctgta aggattcctt ctgctttaac
    16081 cattccttct atatcttctg gagatgtctc tagtctaact aaaactactt tttctccttt
    16141 ttcatttgct tttactacat catcagcatt gaaataaact ttaccacttg ctgcacctgg
    16201 tgatgctggt aaaccttttg ctataacctt agcttctttt aatgccttat cttcaaattt
    16261 gggatgtaat aattggtcta attgattagg ctcaattctc atgatagctt ctttttcatc
    16321 aattattcca gcttcaacta aatcaaccgc tacatttatt gctgctgctg ctgttctctt
    16381 accatttcta gtttgtagta tatataatct ttcattttct atagtaaact ctatatcttg
    16441 catatcttta taatgacctt caagtatatg agtaattttt acaaattcat cgaatacagc
    16501 tggcattaca tctgcaagag ttgatatatt ttgaggagtt cttatacctg caacaacgtc
    16561 ttcaccttgt gcattcatta aaaattcacc aaataattta ttttctcctg tagctggatt
    16621 tctagtaaat gcaacacctg taccactagt ttctcccata ttaccaaata ccattgattg
    16681 aatatttact gctgttccta agttatgagc tatatcattc aatttacggt atacgattgc
    16741 acgaggatta ttccatgatc taaatacagc ctctattgca agcattaatt gctcttttgg
    16801 gtcttgtggg aaatcatttt ttatttcttt tttataaatt gctttaaatt cttttactat
    16861 ttcttttaaa tcatcagttg taagttctgt atcaactgtg tatccctttg cttcttttac
    16921 tctgtctaat acgttttcaa atttatattt tggaacttcc atagcaacat cagaaaacat
    16981 ttgtatgaat cttctgtaac tatcatatgc aaatctttca ttttgtgtag cctctgctaa
    17041 gccaacaaca ctattgtcat taagacctag gttaagaatt gtatccatca ttcctggcat
    17101 agagaatact gcaccagaac gaactgatac taataatgga ttcttattgc atcctaatgt
    17161 tttatttaaa tctttttcta aagtagctaa atgctcttct atttctttta ttatctctgc
    17221 tcttatactt tcattgttaa cataataatc attacacgct tctgttgtta tagtgaatcc
    17281 aggagggaca ggtaagccta tcttagtcat ttctgctaaa ttggcacctt tacctcctag
    17341 taaagatttc atgtctttgc ttccttcacc aaaactataa acatacttag tttccattgt
    17401 taaatccccc attaatcttt ttattttaat ttatatcatt tttattaata gacattatta
    17461 aatttctatt tctttgtata tctttagacc attttctttc attatgtcta aaattatacc
    17521 tgctgtttct tctattgcct tatttgaaac gtttattact gggcatccaa ttcttttcat
    17581 tatctcttct gaataatcta actcctgaag tattctttct aaattagcgt agtttgcttt
    17641 actggacaat cctaaagctt ttaatctttg ggttcttatc tcattcagct tttcaggtga
    17701 attagtaaga cctattatct ttttagtatc tatctcaaac acttctttag gaattggtat
    17761 ttctggaact aatggaacat tagctacttt tatattttta ttagctaaat acatactcaa
    17821 aggagtttta gatgttcttg atattcccac taaaattatg tctgcttgaa gaacacctct
    17881 agggtctttc ccatcatcat atttcactgc aaattctata gcttcaactc tcttaaagta
    17941 agattcatct aattttctaa ttataccagc ttctctttta ggttttcttc ctgttctctt
    18001 gcttatttct cttagtatat ctgtcattaa atctatacaa ctaagacctt ctctttcgca
    18061 atatctttca acaatactgc ataattcttc atcaactaat gtatatacaa ttaaagcgtt
    18121 ttcagctttt gcattctcca aagtttcaag tagtactcct ttattaatca tgtatggaaa
    18181 tctttttatt tcataagttt cattcacata aaactgagac atacatgcct ttgcaacttg
    18241 ttgtgcagtc tcccctactg aatctgatac tgcatatata attaggtttt tcaccagcat
    18301 acctccttaa ttgtcaacta tttctaagaa tagcttagtt atgttggttt ttgatattct
    18361 cccaacaact ctcatatgat ttttgtcttc tcccttatat tcaacaatcg gaatcgaatc
    18421 tacttcatgt tctattattt ttcttgaagc taataaaaca cttgctcctt tagttaaagt
    18481 gactacattt ggagttcttg tcattatcat acctattggc attttattta tatcagaacc
    18541 tcctatagtt gcttttagca agtcttttct ggaaacaact ccacataact cttcattctc
    18601 atctataata actatactcc ctacatcaga taaaaacata gtgactatta catcatatat
    18661 actttcatcc ttcttagcta gtacaggcat gctcattata tcctctactg ttttttcttt
    18721 tacactttta ccaattaaat ttatttcact aactcctgaa taaaaatatc caacttttgg
    18781 ccttgcgtct aatattcctg tcattgttaa tatagctaaa tcagatctaa gtgtagcacg
    18841 agtcacattc aagctggagg caatactttc actcgttatt ggttcatttt ccttaactat
    18901 atctataatt tttagttgtc tttcattaag ctgaataata atcaccttct ttcattgtgc
    18961 tatacttttt atcttttcgc atcttatata gtatattata taactatata taccattctg
    19021 taaacagatt ttttattgaa tacgatatat tttttactaa gttctctcgc ttaaagatat
    19081 ttattatata ttaatggggg atatttcttt aattaataga actatattag tattacagtt
    19141 aatattataa tacataattg gtcattttaa ttcattttat attaaaattt ttaataatat
    19201 tgtttcaatg tgaatataaa attgcagaac taattcaaaa taatttttac gactacaaat
    19261 aacctataat atacttttaa agaaagtatc ctaacaagaa tatataaaaa tttaaaaggg
    19321 ttgatttaaa aataaccatc aacccttttt actaatatct atttatatta ccatcaaatc
    19381 ttacccaaat attttttctt gcaattgtct taaatgatat aaagtagtat ctttttctat
    19441 ctctaccata tcgtaagtta taacttcacc ttttttaata tcttttacaa ctttagtctt
    19501 tttacttatc aaaccaatag gtacagcatt catagccttt gctacatcgt atttttctat
    19561 agtaccatag catgtatatg ctccatagcc atccataaac tcaccttttt tcaaatcttt
    19621 cttagctata gtcataactt ctgctactag tccagcttta ggtactattg tagccttatg
    19681 gtctaaaact gccatagctg ctgataaagg agtctctaaa ctacataaat gatatggtct
    19741 atataatatg taattaggtc catcacccat acttaaatat tttagttcat cattaactgc
    19801 ttttaattta tgggcaacta ctacgaaaac tccaggtgct ataccattta caaaatcaac
    19861 tacaccataa ttatctaata ctccaccttc gctttttaat gcatatttct taggtacttc
    19921 attaactgta gcttcaatac catgaccacc tcttacatca ggcacaaatc cagttgcatt
    19981 acacattaaa gccatttcta ccattgtttt agttccttct ttaaaagaag ctatcatatg
    20041 aggtgaagct ccttttcttt ctgcttcttc tctaactgta tcaggattac aatctaaatc
    20101 taatttatta tttttacctt tacctatcac tctaacttca aaccccattg catctgcaaa
    20161 atcaaataat tccattactg cacctggctc atctcctgca gaacctgtaa atattactcc
    20221 agcatcatct gctaattttt taagtattgg tccaacaaca caatcagttt cagcatttaa
    20281 cataactata tgttttttat ttaaaataga gtttaatgct atctctgcac caattgcaat
    20341 acccccagtt gcatctataa ctacatctat tgacttagtt ttacaagcca caagaaagtc
    20401 attacatgtc aatacttttc cttcttgtaa ttttatttct gcatcctttt catttgttac
    20461 attatctata atttcttcat ctattcctgc atcagtaaaa gctttatgag ccaactccaa
    20521 atttatatct acaacaatag aagcttcaat acctttcata tttgccattt ggcttaccat
    20581 tccatttccc atatgcccag ctcctataag agctactttt atttttttac cttcctcttc
    20641 caattttgca agttttctgt ttaaatctaa cataccaatt cctcctgtta taataatttt
    20701 attttcaaat taagaaatag ttattaaatc tccaatagtt atatctggat ttcctatttc
    20761 cacatgtatt tctcctggta aatttacttc acattttcca tcaaatttga ttgtacaatg
    20821 cccaatttct ctaagagtct tctgagcaat atcaccaact gaaactatac aataatctct
    20881 attccctata gtaagtgtat ctcctatttc aacatcactt tttaatgttg ctattgaatg
    20941 aattacagat atatccttta agtcgtcatc attaatggtt tcatcatata taataagaca
    21001 attgctttca tccattaact ctttaactaa actgcctatc ccacttatct taacttcata
    21061 tttcatgaca ttcctcctga taatttatat ttatattgag ttaattaaaa gaaacattta
    21121 ttttttcttt tataaatttt atagcttgta caatactctc ttcatcttta aatttttgct
    21181 ctgcactata tatatctaat ccttctattt cttcaatctc tttaaattta gccattactg
    21241 tacgcccttt taaaatttct ccaaacttta taaacccatc tgaatctgaa gatataatgg
    21301 ctatacttcc tttagaaaaa tagcttttct ttttgcctgt agacactata tcaccctttt
    21361 ttttagctct atttaaagtt ttaaaataca atttattctg cttcattata aatatagtat
    21421 ttaaaatata agcaacaaat aaaattacta ataatttcat atttatacac cttccaaaaa
    21481 tcacattatt tttaaatctg tataacaact ataaatgctg tacaatagtt atataaggtt
    21541 tttaagtaac acctttttaa attctaaaaa agttctacat ttacttatct tattcgttaa
    21601 ttttacatct tggcctatct ttaaaacttc ttcataaatt tttactaata agtctgagtc
    21661 tatcttattc tcacatggaa acattgtcat aagaattatt tttatacttc tatttgcata
    21721 ttctattggt tcttctaaaa ttccaactgc cattaaaaac ttatctgatt tttcatttat
    21781 agaatgagga aataataaac ctttatcaaa tactgtggga gatttttctt ctctattgac
    21841 tatatttttt ctaaacttct catcaataca tcccaaatcc ataagatggt ccatcatttt
    21901 ttctaatgag gcaagtatag tcttttcatt taatatcata aatttatctt catctaacaa
    21961 attatttatt agtaaatttg tagaattagt agtgtcaata ttcccttctc ttaggcacaa
    22021 tactgttttt aattgttctc ttagtttgtt ttcatcaaat attgtattta tttttaatac
    22081 tattgtattt atatttgatg tgtctaaatc aatagtcgta aaaactatat cgtattcatc
    22141 taagttaata tttctcaaat taaaacttga aagagtatca attttaggat tatttcctat
    22201 cagtttttct actcttattt tcaatagttt tgctgttcct agacctgtac tacatataat
    22261 tgctattttt tttattgaat agaatctact gctatttctt tctaaatatc cactgaaatg
    22321 gattgctata tatccaatct catcttctga aacttctaag ttatactttt tctctattac
    22381 ttttcctgct atttgtgcta atttaaatgg aagtatacaa ttttgcttaa tctcttctaa
    22441 caaaacattt tttaacttta tattaaataa tagcctgttt aaagtaaaac taagatgata
    22501 tcctagacct actcttaatt catcctcatt tataaaaata cccatatcga catatatttg
    22561 attaaatata ttttccatca actcatcaat gtttttacta attctactac tatttaaagc
    22621 acattctgat gctggtgcat ttccactaac taacggtata gttaaataaa taatttcttc
    22681 atcatttaaa gtacattcaa acattcctga tattatatgt tttatttccc tagcaataaa
    22741 gtattcatca tatgatttaa gtaaatcaaa ctttacatca taatctttta ctttcttttc
    22801 ttcttttact ctatagacta aaactactat gtagttaata acttctctta acatttcatc
    22861 tgtaacataa aaattatttt ttttaaataa agtcaatagt ttttcttcta aatttatgat
    22921 ttcttctttt ttgattcctt taaaatactt tgaattactt aaatcttttc ttgagttttt
    22981 atacaactga tttaatataa acagtctaat atcaagttca tttccattta atttcatacc
    23041 tgtattctgt tttttaatca agtttaaatt atatgaagcc aaaacttttt ctacatactg
    23101 gaaatcattt actaaagtag ttcttcctac acacatctca tcagctaaac tatcaagtgt
    23161 aataaatcca tctagttcta tcaagtaatt tagtataaac tctactctat cttcttttga
    23221 atttaatgaa aatacatttt ttctattcat atcaataatc ttattgagta tctgctcatc
    23281 ttttatttct agtttatagc cttgacactt tatatatttg attactgcta tatcattacc
    23341 taaatcttta ttcaattgtt ttatatacgt ccttacactt ctagttgtga cattcaattc
    23401 atttgctatt gagttaatac tcacaaactc ttttttacta cacataatca atatcttaaa
    23461 tatcttgcgg tcaatagaag gaattttctc agttacactc attttgtatc tcctccttga
    23521 tttcattcta gtacaaattt gaaaccaaat taatacataa aatttcactt taataaagga
    23581 aaatttagta ttaatctaac tcgataatta cagctaatta atagtataaa agctagtctt
    23641 tcattaacaa aaaatcttga ctagcttaaa tttatattta caattttatt aattaattcc
    23701 aagccttata ttgacattaa atattatctc aatgcaaata ctgcaactcc atatgcaata
    23761 ataacagcta ttggaccagt tattaaacga gagtaaaata aagctggaac accatactct
    23821 acagtttctg gttctgcttc tcccaaactt aatcctactg gtacaaaatc acatccagct
    23881 tgcccattta ttgcaaataa agctggaagt gctaaatatg ctggtattgc accaagacca
    23941 atttgtgtac ctacaagagt ccctactact tgagctataa ctgcaccagg tcctagtatt
    24001 ggtgataaga atggaagtgt gcaaattact gatataatca ataatccacc aatattacct
    24061 gcaagtggag atataactct tgcaatccag tctcccagtc cagatgctaa aataattccc
    24121 ataagcatac ttacaaatgc cataaatggt attacgttat tcattaccat ttggatagta
    24181 tctcgacctg catcataaaa ttttgatact acttgccctg ccccttgacc aatagaaagt
    24241 attatgttct ttttagcata ttcaccttgt gcttttgact taacctcatc ataattatca
    24301 ggctttctaa tagaactctt attttcgctt ttattttctt gagatttttg tggcatatct
    24361 tccccatcta caacacttat acagttagga ttaacatctg atacataata ttcttctgtt
    24421 ataaattttg ctaaaggacc tgtttttcca actggattta catttattgt aggtattttt
    24481 ttctttggat aaactccaca tctagcagtt ccaccacaat caataataac tactgctact
    24541 tcttcatcag aaactccagt tttaaatcca tctacaattt cacaacccag tagttctttt
    24601 atttttaatg ttacttcatg gataccgcca gcagtcattg atagtatctt atttctattc
    24661 ccttcttttt ttatatacaa aggacctccc cagcctgaag ttcccttttc tatttttaat
    24721 attttattct cctccatttt atctacctcc taagccttat ttgttgctct attttttctt
    24781 gttagaaaag catacatttt ttctgttact atacctctta taaaaattac aattagacca
    24841 actataaaat accacaatgc cagtgtagta gtttctttac ctaaagtagt caaacctgct
    24901 gatattccca accatacaaa aagttctcct gcatttgcat gtggaaaaag acctgtaatt
    24961 ggatgtacaa atgaaactgc tgcatcataa aatcctgctt tgtgctcttc ttttaaaaat
    25021 ctaccaaatg tatagcacat aggatttccc aagaaaaata ctgatactaa tggaagtaga
    25081 atatatcttg taaaagagtt ctttcctagc ttagaagccc aattattaac tctttcctca
    25141 ccaattatct taatcaaaga gttaatagct accattaata caattagcgt tggaagagta
    25201 cctgaaacaa gacctataaa ttgttctccc cctttctgaa acaaaccgat aaaccactct
    25261 gcacctttac ttagacctgt aataacgttt tccataaaat tataacctcc ctataatatc
    25321 tttaaatttt taattttact gcttaccata aattttacaa attacatctt ttttctgtac
    25381 tatatgctaa tattgtaaat gtttttttat taatatcatt ttaatataat atttttgata
    25441 aataaataca tcttttttca attttataaa ggaaaaaatt gtactaatta gaattttcag
    25501 aaatatacaa ttcttatttc gactatattc taaattcttc ttagaaattt aagaacttat
    25561 ttttatttct tttattatta aaattatcta aatctgataa aaataaaaaa tagatgctct
    25621 tttacgaaca tctatttctt aatcaaatta tcttttagtt ttcttataga ttattttata
    25681 ggtatataaa tatcaatttg tgagttcaca ttactaggac ctaaaaatcg ttcatcatat
    25741 acttcaaaat catctcttat atcaagttct tcttttgaac ttaaaagcca agtaccccat
    25801 atatattcat aagttttgtc taaattatct aagctaccag tatgagtaaa tatagcatac
    25861 tttccttcct taatagtttt tgaaacaaat gaatttggaa tcacatcata actattaact
    25921 tcaagtccta ttatctcatt aaactccata ctttcactaa ctaggtgtat ttcagagatt
    25981 gcttcacaga ttccaaaaac tctctttgat ggcaaagtat ttgggacaat atgatgtact
    26041 tttctgaatt tctcccacaa gtcaggtaat gaattatttt ctaaaattac tttatccttt
    26101 atacctatta cctttatttt ttctatttct tttataactg gtttaatcgt tatatttcca
    26161 acaagatgtc tcataaaatc taactctaat tttttctttt taccaacaaa tacatcaatt
    26221 ctattttttc tatattctat agggctaacc ttgtatatag atttaaatgc tctgctaaat
    26281 gcttctgaag attcaaatcc actaccaata gctatatcta tcactttttt atcactatat
    26341 aaaagttctt ttgtactact tactaatcgt ctttttttta tataacttcc tacactctca
    26401 ccaaacatag acttaaataa gcgtgttaaa tgataataag aatatccagt ttcttttgcc
    26461 acatcattta cagttatatt ttcatgtaaa ttattctcaa tatacattac agctttttca
    26521 aaatacttta agtaatccaa tatggcacct ctcttctact taaactcttt aaatatgtaa
    26581 aatagcacta ttttttacta tactaaaata ttataactaa ttcaatacga taaataaaaa
    26641 cttttagttg atttatatat cctaattatt tagaataatt gaatacattt tttattcaat
    26701 gaaatcaaaa aagtcagatg gtgctttttc aatatttatg tttatcctct gtgttataga
    26761 tttaatgtca ttttttaaat agtagctctc ccatttattc tctagtccac cccataaaat
    26821 attaaacttt cctatattta aatccataac tatacttttt aaagttccaa aaaaatcatt
    26881 atagtaatta caagataagc catttggata ttttgaagac aataaattca ttaactcttt
    26941 ctctccaatt tttttagact tatttatata ttctttaatt aatttatatc tatgaataga
    27001 atttttcatt gattgtggat ctaatttatg aagtttatca atatgtgtat gattagtaga
    27061 gtgtaaaaaa tattctcttt tactttctaa actgttgatt atatttactt cttttttccc
    27121 atctaaagtc tccacaagtg caatgtttcc agacttatct gcaagcaata aattaatatt
    27181 gtatgcaatt ggcatatctt ttaaatattc taatgcttca tctacatctt tgcaattttc
    27241 taacaaatat ctaataacag cccaaaactg taaacctaca attgctggtt ttcttaaacc
    27301 atctgaattg cctacaggaa taccacatga tgactgactt acacctaagc cacattcatt
    27361 cataccttca cctcttccaa attgcataat ggtagtacct atatgtgcat attttccatt
    27421 taccttagtt ttacataaaa caaaatcctc catattgtgt gagaaatcga aattacgtgc
    27481 aaatagtaca tgcccatttt cagttaattc tggtgctaat gcaacttgac tacaacctgg
    27541 tttaagataa gtcatagtat aatataaaat ttgattacaa ttcacttgaa ttgcatcagc
    27601 aaaaccttgc aattcttcat taagacctgg acaatattta tcaaacaact ctatcatctc
    27661 tttttcttct tttttgctta ccagattaga tttattaatc tgaccttcta ttatctctgg
    27721 catattagca accaattctc ctagtctgta tcctatctca taatttgtac cactcaattc
    27781 aatatttctt acttctaaag tttccacatg tacccctcct atatatctta taatcgattt
    27841 tatcattcga attgtaatca ttcatgatat tttgtgctac aagttaaaaa aggtcatata
    27901 aaaacagata agcctctttt tcatgtttaa aataataaaa atatatttaa tttcatacaa
    27961 aaaagagtgt ctcataaact aaaaagttcc ttttgagaca ctcttcttat tttaaagagt
    28021 ataattaaaa atagtctaag ttctactctt ccttcatttt tttcaataac tctggatttt
    28081 caaataaatt aaatcctgtt aaagctaata tttttatacc atcaattact ctatcttttg
    28141 catatgaatt tatagaagcc tccaaaaatt cttttgtatg tagttcttct ccattttcag
    28201 aaaacttcat caaaggatgt atacatggca ttatttgact taaatttccc atatctgttg
    28261 aacctaacat tacttgttca tcttcatata ttccaaattc ctccataatc tttgtaaata
    28321 tgtctaccaa aactttattt tcatttaatc cttggcaagt tggttcaaag tgataaaatt
    28381 caagttcaca tccagtggct attgcagcac ctctagcaca atttttcact ttttctacta
    28441 cttcatcaaa gtaattttgt tcttttgccc ttatctctaa tcttactttt cccatatcag
    28501 gtattacatt tgcagcagtt cctgcttcta gtataatacc atgtattctc gttccatcta
    28561 ctaattgttg tcttagtgca tttatattat tatatagtag tactactgca tctaaagcat
    28621 ttattccgtt atatggctta gctcctgcat gagatgcttt tccagtaaat atgaaatcta
    28681 gacctcctat tgctataatt tcaggtgtta cacaagtttt tgaattagga tgtatcatca
    28741 ttgctgcatc atagccatca aatacaccat gttctatcaa gtaaggcttt ccatctccag
    28801 tttcttcagc aggtgttcca attatactta cttctccctt gtaagtatcc agtatacttg
    28861 aaagagctat tccagctcca actcccatag atgcaattaa atgatgtcca catgcatgtc
    28921 cattgcctgg aagagcatca tattctgcta taaatgctat tttaggacca tctccatttt
    28981 tcttggttgc tttaaaagct gtagccatac ctgccaagtt ttcctcaaca tcaaaaccct
    29041 cttctcttaa aaattctgat atttttttag cagatttata ttcttgcaaa gcaatttctg
    29101 gttcatcata taaagattga catagcccta gaattttttc tttctttgaa tcagccactt
    29161 gtattattct ttctttcata ccaatcttac ctcccactaa atttatttag taatttttat
    29221 attttattat aactttaatt taactcatgc ttatcctctt ttaagtatct attttcccaa
    29281 aactcagcat ttcttatatc taactctata gggttaaaag ttctatcact cctttttatt
    29341 cccatcttct tctgtcttat ataatcataa tatgccttta tagctggttt ttttagtgta
    29401 aagatggcaa ttaaatttat atatgttaat acacctatac atatatcacc taatgcccag
    29461 cttgtaagtg gttctataaa acaacacatt gctgcacatg ctataagcat aaatctacag
    29521 acattaatta tgtacttttc atgtttttct gctttaattc tagttactaa gtaagataca
    29581 ttagtctcca tcatatatga gaaggctaat aaagttgtaa atgtaaataa taataaaact
    29641 attgctataa aaatatcacc taattgtgta tgtaatgtat ttattgcact ctgtacatat
    29701 tgtggtcccg gttctacacc tggtagtgct gtgcaaacac ctgtaacatt atacattcct
    29761 gttattacaa tcataatacc tgtagccata catattaata atgtgtctac ataaacagaa
    29821 aatgcttgaa ctaatccttg tttggctgga tgacttactt ctgctgctgc cgcaccataa
    29881 gtcccagaac cttctcctgc ttcatttgag tatatacctc tttttactcc ccaagatatt
    29941 gctgaaccta gcattcctcc aaaaactgaa ccaaatccaa atgcagattt aaacattgtt
    30001 acaatagcct ctggaactaa actaatattt gttataatta ttactaatgt cataataagg
    30061 tatgctccac ccattatagg tactactttt tcagcaaata aaccaatcct tttaattcct
    30121 ccaaaaatta ttatcgctaa tgcaatagaa gatgctactg ctattgtcat ttttgaaaat
    30181 ccaaatgctt gattcattgc tagatataaa gtgtttgatt gtgtaccagg cataaaaaat
    30241 gtataactta ttattgcaac tattgaaaat aaaactgaac actttttcca cccaagacct
    30301 ttttcaaaat aatatggagg tcctcctcta tactgcccat catgctcatc tttatatatt
    30361 tgagctagag tagattctac aaatgcagtt gctgaaccta atgtagccat tatgcacatc
    30421 catactaatg ctcctggacc tcccattgct attgctacag ctacaccagc tatatttcca
    30481 gtccctattc ttccagccat agatgtagca aaaccttgaa atgatgatat accaacatga
    30541 gaactatctc cttcaagcaa gagagtaaac atttctttta catctcttat ttgaaaaaat
    30601 tttaatttca atgaaaaata taatcctata cacaaacaca ttgcaactaa tacgggtgac
    30661 caaacaattc cattaatggt gttaactaaa tcggtcactt aactcaccac cattcatttt
    30721 atatttatag acaataattt aaacagctat atttcaatat tttacataat ctttatatta
    30781 atatatctaa aaaatctacc ttctatttat ttatattatc tttaatctac aatctacaaa
    30841 tatttatttc taaaatcaag agtattaatc atatctaaac ataaatttat aaaattttta
    30901 acgacgtgtg tttga
//
LOCUS       DAELNY010000027        30800 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.027, whole genome shotgun sequence.
ACCESSION   DAELNY010000027 DAELNY010000000
VERSION     DAELNY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.027"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            209..3757
                     /locus_tag="KPE55_001083"
     CDS             209..3757
                     /locus_tag="KPE55_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0492179.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3942..5876
                     /locus_tag="KPE55_001084"
     CDS             3942..5876
                     /locus_tag="KPE55_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG0492180.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5982..6977
                     /locus_tag="KPE55_001085"
     CDS             5982..6977
                     /locus_tag="KPE55_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0492181.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7005..7733
                     /locus_tag="KPE55_001086"
     CDS             7005..7733
                     /locus_tag="KPE55_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0492182.1"
                     /translation="MEVTLSCVILLFLWQIIALKIDNDIFLPTVGQVFNSIKEIMLNS
                     SFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRNLLKPINALTRSIPTMILVV
                     LALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNLLEMANVYKIRFIDKVLKIY
                     LPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTMIQTEKINFNTSGIFAWIII
                     VLLISAILQIAQKFLARRAFLWKR"
     gene            7721..8419
                     /locus_tag="KPE55_001087"
     CDS             7721..8419
                     /locus_tag="KPE55_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492183.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8542..9015
                     /gene="ybaK"
                     /locus_tag="KPE55_001088"
     CDS             8542..9015
                     /gene="ybaK"
                     /locus_tag="KPE55_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG0492184.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9212..10051
                     /locus_tag="KPE55_001089"
     CDS             9212..10051
                     /locus_tag="KPE55_001089"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG0492185.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10064..10612
                     /locus_tag="KPE55_001090"
     CDS             10064..10612
                     /locus_tag="KPE55_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG0492186.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10678..11859
                     /locus_tag="KPE55_001091"
     CDS             10678..11859
                     /locus_tag="KPE55_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG0492187.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12154..12540
                     /locus_tag="KPE55_001092"
     CDS             12154..12540
                     /locus_tag="KPE55_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492188.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12690..12953
                     /locus_tag="KPE55_001093"
     CDS             12690..12953
                     /locus_tag="KPE55_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0492189.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13082..13924)
                     /locus_tag="KPE55_001094"
     CDS             complement(13082..13924)
                     /locus_tag="KPE55_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0492190.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14219..14953
                     /locus_tag="KPE55_001095"
     CDS             14219..14953
                     /locus_tag="KPE55_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0492191.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14963..16096
                     /gene="nagA"
                     /locus_tag="KPE55_001096"
     CDS             14963..16096
                     /gene="nagA"
                     /locus_tag="KPE55_001096"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0492192.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16195..16944
                     /gene="nagB"
                     /locus_tag="KPE55_001097"
     CDS             16195..16944
                     /gene="nagB"
                     /locus_tag="KPE55_001097"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG0492193.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17119..17391
                     /locus_tag="KPE55_001098"
     CDS             17119..17391
                     /locus_tag="KPE55_001098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492194.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18051..18755
                     /locus_tag="KPE55_001099"
     CDS             18051..18755
                     /locus_tag="KPE55_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0492195.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18757..20244
                     /locus_tag="KPE55_001100"
     CDS             18757..20244
                     /locus_tag="KPE55_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0492196.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20449..22062
                     /locus_tag="KPE55_001101"
     CDS             20449..22062
                     /locus_tag="KPE55_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0492197.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22425..23255)
                     /locus_tag="KPE55_001102"
     CDS             complement(22425..23255)
                     /locus_tag="KPE55_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0492198.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23328..25055
                     /locus_tag="KPE55_001103"
     CDS             23328..25055
                     /locus_tag="KPE55_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492199.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25092..26954
                     /locus_tag="KPE55_001104"
     CDS             25092..26954
                     /locus_tag="KPE55_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492200.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27287..27976
                     /locus_tag="KPE55_001105"
     CDS             27287..27976
                     /locus_tag="KPE55_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG0492201.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28072..28758
                     /locus_tag="KPE55_001106"
     CDS             28072..28758
                     /locus_tag="KPE55_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG0492202.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28775..30682
                     /locus_tag="KPE55_001107"
     CDS             28775..30682
                     /locus_tag="KPE55_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG0492203.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKHSGNQSDEKEVNAELTSSVVKA
                     NTDNENKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT        12175 a         3217 c         5390 g        10018 t
ORIGIN      
        1 aataaagtta taatttttaa tattaattaa gaatgatgaa atatgaataa agattttaca
       61 aataaatctt aagctatctt atttaattct aatacaggaa gaaggtaaat gtatattgaa
      121 aaaatcatag aaatagaagt aaacaattta aataaataca aaggtttgca aaagaagtaa
      181 tataattata tacaatgagg atgtgagtat gatgaacaga ctaaagccaa gaattttagg
      241 tattagtatc ttgtttatta ttataattat tttaacatgt ggagctgttt tcaagtttaa
      301 atttaaatta gataataaat taaaagaaac cacacataca tatttgtctg agtttacaga
      361 acacaatata attaatctca agacaagact tagtggtcaa tttgacatgc ttgaatctat
      421 tgctaacttc ataggaaact tgaataatat aaatgatgaa atggtgattg atttaatgga
      481 ttctgatgta aagcgaagtt ctttaattag aatgactatc tctacattag atggggaatg
      541 ttattctaat gatggaatta cccataatgt tcaagataga gattatttta aaaaagctaa
      601 aaatggagaa aagaatatat cagagccttt aaaatctatt gttaataata aagaaataat
      661 agttttatct accccagttt ataaaagtgg gaaaatagtt ggtgttttaa gtggaattta
      721 tgattcaaaa aagttaaatg agttattagg attatctgca tttgatgaaa aagcagatgt
      781 ttttgttgca aagaaaaatg gagatatagt tgctcaaaaa acaaataaaa atagaaagac
      841 gctatcatgt atatttcagc tttttgacaa agataattgt gatgaagatg attgtctaaa
      901 aactataaaa gaaaatatga aaaatgataa gtctggatat tttaattata acttagataa
      961 tgaaaaattt atgttaaact atcagccact tggagttaat gattggtatg ttttttctgt
     1021 tgtgccaaat aaaatagtat ctattcagtc agagaatata tcttatgatg tatatatttt
     1081 tgcattagaa atactactag ttatattgat ttttataata tatatagcat gtattataaa
     1141 taataacacc aaaattatta taaatgaaaa agaaaaattt aaagtactca ctgataatat
     1201 attaggaggg gttaaaacct gtttaaaaga tgaatatatg actatgacat atgtaagtca
     1261 ggggttttta gagcttacag gttactcttg tgaagattta aataaaatat ttaaaaatag
     1321 atttaaagac atgatttata aagatgattt agacaagttt aattgtatta ttctaaatca
     1381 attagaaaaa caagataaaa ttgaagttga atatagaatt gtaagaaaag atggaagtat
     1441 aatttggatt ttggagagaa gtaagtctat aaatgataat aaaggaagtg agtgtattca
     1501 gggagtttta actgatataa cagaacttaa gaaaatacaa gaagaattag attcaaaaag
     1561 atttgaaata caggctatca atgatagtat tgttggtgga atagtgataa ctgagataaa
     1621 tgatgatttt gatagtatat atttaaatga aggttattta aatatgattg gatatacacg
     1681 caaacaactg gaagatgagc taaataataa attaatcaaa cttattttta aagatgatag
     1741 aacagaatta attaatagta taaacaaaca actcaaatta agtgatacat ttgcatctga
     1801 acaaagagtt gaaaaaaggg atggcactat tatatggatt ttattaaaag gaagaaagat
     1861 gatttatgat aatggaaaat ctgtactttg ttgtgttatt gtagatatta cttctgctaa
     1921 attattagag caagaactta aaaagagtga agaacgtcat gaaatcattc taaagcagac
     1981 gcaaaatatt ataatagaat gggatataga aaaagataaa atgaatatat cacctaattg
     2041 gtataataag tttaaatatg accctataac tgaaaacgca acaaaaaata taattaaatc
     2101 agtagatata tatcatgatg atatggataa gtttatttca ttaattaaac ttacttcaca
     2161 aggtagtgag tatgaagagt gtgaaattag acttagaact agagatacta tttacgcttg
     2221 gtttagtata aaggcaacaa ctttatttga taataatggt aaaccttata aagtagtagg
     2281 aagtttgact gatattgatg aatcaaaacg agaagctgaa aagttgaaag aaagggcaga
     2341 aaaagaccct cttacagatc tgtataataa aaaagcatca caatcactta tagaggagta
     2401 cataacattg gataaaaaaa gaaaaggtgc tttaatgatt attgatattg atgactttaa
     2461 aggaataaat gataatttag gacatttata tggggatgct gtattaagtg aaattgcaaa
     2521 tgacctaata aatctattta gagcaaccga tatagtaggc agaattggtg gagatgagtt
     2581 tattgtattt ataaaagata tttctgaaac aagagatatt ataaataaag cagaagaatt
     2641 ggtaaaaaca tttgaaaggt cttttttagg taaaggacga aattataaaa tatcattaag
     2701 tgtaggaatt gctaagtttc cacaagatgg tatggactat atgacactat ttagaaatgc
     2761 ggatatagct ctttactcag ccaaagggaa gggcaagaat tgttatacat tgtatgataa
     2821 aaaatttgat aaagtccaat atataactaa tacaagaaat gcaggagata gtgataatgt
     2881 agagatgtct tttaaagaac atatcacaaa ttatatattt gatatactat atgaatcaag
     2941 tgatatagaa gtggcagtaa atttaattct aagtattgta ggtaaacact ttaatgtgag
     3001 tcgcatgtat atatttgaaa attcagatga taatcgagta tgtaaaaata catttgaatg
     3061 gtgtaacgaa ggtgtaacct cggaaattaa taatcttaaa aatatttgtt acaaggattt
     3121 aggcaactat cttttaaatt ttaatgcaga tgggttattt tattgtaatg atattgaaaa
     3181 tttaggtagt gatgtgaaaa aagtacttaa gccacaaggc atacgttcaa tattacaatg
     3241 taaaatttta gagggtaatg aatttagagg gttcatagga tttgatgaat gttcatataa
     3301 tcgtatgtgg actaatgatg aagtgaacac tttgacattt atatctaaaa cgctcagtat
     3361 gtttttatta aaaatgcgta ttcagagtaa gctagaagag tctttaacaa ttcaaaaatc
     3421 tgtgatggat aatatggata tatgggcata tgttgttgac aggaatacct atgaattact
     3481 atttatcaat aaaaaaatgc ttgagtcagc accaaacact catgtaggtg atttatgtta
     3541 taaagcgata tggaataatc aagataaacc ttgtaaaaaa tgccctatga tatatttaaa
     3601 agatggtgat gatacttata aatcattcat gcataataaa aagtttgagg tacaggcaaa
     3661 tataactaca actactttga aatggtttga tggtaaagat gcatgtttaa tatttggaag
     3721 tgaaataact agtattgaag ctgaagaaaa tacttaaaat actgtgttat atatatactt
     3781 aaaatcgcaa tttgaattta ttagacttaa aatgtctata taaatcattt aagttgtggt
     3841 tttaagtatt ttttatgata taattaagcg ttaagaacaa aattaaaaga tacttttgta
     3901 taaaaaaata tttgttaaat atatattgag caggtgataa tatggaaaat aagtttttac
     3961 caataagcaa acaagatatg atagatagag gatgggaaga attagatttt gttctagtta
     4021 caggagatgc ttatgtggac catcatagtt ttggaacagc aataatatct agagttttag
     4081 aaagtgctgg gtataaagtt ggaataatag ctcaaccaga ctggagaact acagatgatt
     4141 ttatgaagct aggaaaacct agacttgcat ttctagttaa tgcaggaaat atggattcaa
     4201 tggtcaatca ttattctgta agtaaaaagc atagagaaaa ggatatgtat tctccaggtg
     4261 ggaaaatggg ttatagacca gatagagcta ctatagtata ttgcaataaa ataagagaag
     4321 catacagtga tgttgcaata gtaataggtg gaattgaagc gagcttaaga agatttgcac
     4381 actatgatta ttggagtgat aaggttagaa agtctatgtt aatagatagt ggagcagatt
     4441 tattagttta tggtatgagt gaaaagcaga ttgtagaagt agccaatgca cttaatgatg
     4501 gatatgaccc aaaatacatt agacatattg atgggacatg ctatatttca gatacattgg
     4561 aagaaatata tgataaatat atattaattc catcatataa agaaatttgt gaagataaaa
     4621 tgaagtatgt agaagctttt aaaattcaat atgatgagca agacccattt agaggtaata
     4681 taattgttca acctcatgga agtaagtatc tggttcaaaa taagccagaa aaaccactta
     4741 gtagagaaga actagatgaa gtatatggat taccatatca gaagacatac catcctgtgt
     4801 atgaaaaatt tggaggaata ccagctatag aagaagtaaa atttagtata gtaagttcaa
     4861 gaggctgttt tggtagttgt tcattttgtg caataacatt tcatcaaggt agagcagtgc
     4921 aaagtagaag tgaaaaatca attattgatg aagcaatagg aataacaaat ttagatgatt
     4981 ttaaagggta tatacatgat gttggaggtc caacagctaa ttttagaaga ccagcatgta
     5041 aaaaacaaat tactaaaggt gcttgtaaaa atagacaatg cttatctcca tcaccatgta
     5101 aaaatttaga tgcagaccac agtgaatact tacatttact tagagctgta agaaaattac
     5161 ctaaaataaa aaaggtgttt gtgcgttctg gaattagata cgattatgta atggcagata
     5221 agaacaataa atttcttaga gagttgatag agcatcatgt aagtggtcag ttaaaggtag
     5281 ccccagaaca tatatcagaa gaagttttaa agtatatgca aaaaccagca ggaaaaactt
     5341 atgataaatt tagacagaag ttttttgcta taaatgaaga attaggtaaa aagcaatatt
     5401 tgatacccta tttaatgtct agtcaccctg gttctacctt aaatagcgct atagagttag
     5461 cagagtattt aagagatact cactaccaac cagagcaggt tcaggatttt tatccaactc
     5521 cagggactct atctacaacc atgttttata caggtattga cccgcttaca atgaagccag
     5581 tttatgttcc taaatcaaaa cgtgacaagg cgatgcaaag ggctttatta caatatagag
     5641 cacctagaaa ttatgatttg gtatactctg cgttagttga agcaggaaga gaagacttaa
     5701 ttggttttgg acatagatgt cttataaagc caaaaaatga aaagccttat tttaatagaa
     5761 ataattctaa aaaaaatgta tctaaaggaa caaataaaaa caaaaaaact aatacaaata
     5821 acagaagtaa taaaaatcaa caaaaaagtt caactaaaaa aaagaaaaaa cattaaagta
     5881 taaataaaaa tacttaaaaa agaaggatta ttcgataaat taacagaaat tagtataaat
     5941 aaatataatt attttaatga cgttaccttt gggaggaaga gatgaaaaag tatatttcta
     6001 taatattatt agtggtgttg actatggttg ttttggtagg atgttcacca ggaaaagata
     6061 accctaaaaa taaggagcta tcagttgtca aacaagtaaa agtagctgtt ccagatggac
     6121 taccagcagt ggcaatagct aaacttgcaa atgaaaatcc agagattaaa gaaggatatg
     6181 aaacagtgta tagcattgaa aagacacctg aagcgatttc cactagagtg atgaaaaaag
     6241 atgcagatat agctatagtt ccttcaaaca tggctgcaat agcgtataat aaaacttcat
     6301 catataatat tgttggaact gtaggaatgg gttcacttta tttggtgtca acagaaaata
     6361 taaaagatta ttctgattta gttggtaaag aagttggatg tactggaaaa ggacttactc
     6421 cagacataac tataaaaagt cttttgttac aaaaggatat aaattattca gatataaaat
     6481 ttaattatgt aaattcagca agtgaactgg taccattatt ggctactggt aaagttaaaa
     6541 caggtattgt tccagaacct gcacttagtg ctttgatgtc taaaaatcca gacataaaaa
     6601 ttataaaaag tttaaatgac tcatggaagg aagtaagtgg ttcaaaagat ggttatcctc
     6661 aatctacttt aattgtaaag tctgaatttt taagagataa taaagatttt gttgattctt
     6721 ttgtaggtca attatcaaat agtatagatt gggcaaacaa aaatcctgaa gaattaggtg
     6781 catattctga aaaaataaaa atatctactg aatcaaagat aataggaaaa tctcttgaaa
     6841 gagctaattt aaaatatatt ccagttaagg acatgattaa agattataaa aattactatg
     6901 agaaattagc taattttgat gataagacat taggaggcaa agtaccagat gaagcaatat
     6961 actttgttga gaaataaaaa aataaagaac aatttataga taaaatggag gtaacacttt
     7021 cttgtgttat cctcttattt ttatggcaaa ttattgcttt aaaaattgat aatgatattt
     7081 ttttaccaac agtaggtcaa gtatttaata gtataaaaga gattatgcta aattctagtt
     7141 tctatataga tatactttac tctatgggga gatgtttttt tagtttttta ctggctatga
     7201 tttttgctat tatactttca atagtgtctt atttaaatag attttttaga aacttattaa
     7261 agccaatcaa tgctttgaca cgttcaatac caactatgat attggtagta cttgcactta
     7321 tttggtttga aaaggatagt acccctttta tagttggatt tactatagtt tttcctatat
     7381 tgtatgataa cgttttgggc gctatattga atatagacaa gaacttatta gaaatggcta
     7441 atgtgtataa aataaggttt atagataagg tgttaaaaat ttatttacca gccataaaat
     7501 ttcaaattat atctatacta gtatcaacat tctctttaag tttgaaagtt gctatagcag
     7561 gagaggtata tagtcaacct acatatggta ttggaactat gatacagact gaaaaaatta
     7621 actttaacac atctggaata tttgcatgga taattattgt tcttttaata tctgctattt
     7681 tacaaatagc tcaaaagttt ttagcaagga gagctttttt atggaaaaga taaagttgag
     7741 tattgaaaat ataaataaaa gatatgatag tcgtattata tttagagatt ttaatattga
     7801 tttttatgta aatgaggtaa attgtatcct tggaaaatct ggttgtggaa aaactacact
     7861 cttgaacata ataagtggga ttattaaaaa tgacaccaac aatctaaata tcaaagaaaa
     7921 tttaaataga gtaggaaata agcttgaagc tagttacata tttcaagatg atagacttat
     7981 agactggtta acagtagaag aaaatataaa gatagttgta aataaatact ataataaaac
     8041 tcaattaaat aaaatttgtg atgaatactt ggaattggtt ggaattagtg attataaaaa
     8101 attctatcct cagatgctta gtggaggtat tagacaaaga gtaaatattg ctagagcttt
     8161 tatatatcca tcaaaaaata ttataatgga tgaacctttt aaatctattg atgcaaagaa
     8221 tacacagctt ataatggata atttcagaaa tatattaaga aaagaaaaaa gaactgtgtt
     8281 atttgtaact cataatattg aggaagcatt gtttttagca gataggatat ttattttagg
     8341 agactctcca attagaataa aaaaaatatt aaaaaattct aaggaattgg aaaaaaatga
     8401 agttttgaag ttgatataga gttataataa tactgtcgtt ttaaaatata tttggttaaa
     8461 atctataaga ctatattaaa atagtacaga atattcaaaa ctgtgtataa tgttcgtatt
     8521 ttataaaaag gaagtgaatg tatggttaag actaatgcta tgagaatatt agattcaaat
     8581 aagattgatt ataaagtaat gtcttatgaa gtgaaaagtg aacatgtgga tggtgttgag
     8641 gtggcacatg atataggaag agatgtaaat gaagtgtata agacattagt aactcaagga
     8701 gttagtaaga atatatatgt atatgtaata ccagtacatg aaaacttaga cttaaaaaag
     8761 gctgcaaaag tagctaaaga gaaaagtgta gagatgattc atgtaaaaga tataaataag
     8821 cttacaggtt atattagagg cggatgttct cctataggta tgaaaaagct ttataaaact
     8881 tttgtaaatg aaagtgcaaa gaatttagat acaattattg tgagtgcagg aaaaatagga
     8941 tatcaaatag aactatctcc atttgattta caaagactta taaaagtaga atttgtagat
     9001 gtaataaaaa aataagaatt acatataaaa tatagagctt atattcttaa taataggtgt
     9061 tgtagtatta ataaaatgaa taataatttt aaaataaata ttcagttact tatgtgtaat
     9121 tggtataaaa atattgagaa attttaaaaa caatggtaaa gtgtaagtat aatcacgaat
     9181 attttttata aagtaatgga gggatgttcg cttgagaaaa ataaaatctg aacaaatcgt
     9241 cgagcaggta aaaaaacttt gtatagaagc gagcctatat ttaggagaag atgttttaag
     9301 ctgtataaaa gaaaaagcga aaagtgaaaa gagtgaagtt ggaaaaaata tattgaatat
     9361 attagttgaa aatgctgaaa tagctaaaga aaaaaatata ccaatatgcc aagatactgg
     9421 tatggctgta ttttttgttg aaataggaca agaagtttta attgaaggag acacacttac
     9481 tgatgctata aatgagggag taagacaagg gtatgaagaa ggctacttaa gaaaatctgt
     9541 tgtaagtcca attaacagag taaatacaaa agataataca cctgctgtta ttcattatga
     9601 tatggtaaaa ggtgataaaa ttaaaataga atttgcagct aagggatttg gaagcgaaaa
     9661 tatgagtaag atgaaaatgt taaagccatc agatggattg gaaggaatta aaaaattcat
     9721 aatagataca gtttcagaag ctggacctaa cccatgccct cctatggtaa ttggagttgg
     9781 tataggtggg actgtggaca aatgtgctca aatagctaaa aaggcacttt ttagagaact
     9841 aggtgaattt aataaggacg aaaatatagc aaaacttgaa agtgaactat taacagctat
     9901 taataaactt ggtataggac ctcaaggctt aggtggtact acaacagctt tgggattaaa
     9961 catagagact tttcctacac acatagcagg tttacctgtt gttgtaaata taaactgcca
    10021 tgcatcaaga cataagaagg tagtaattta ggaggaaaat aaaatgataa aaataactac
    10081 acctgtaaat gaaattgata tagcaaaatt aaattgtgga gatacaatta gtttgagtgg
    10141 tatactttat acagcaagag atgctgctca taaaagattg atagattgta taaataaagg
    10201 tgaagaatta ccatttgacg tatatggtca aggaatatat tatgtaggac ctactcctac
    10261 aaagccagga gaagttatag gtgctgcagg tccaacgacg agttatagaa tggatgattt
    10321 gactatacct cttttagaaa gaggtcttag attgatgata ggcaagggaa aaagaagtga
    10381 tgaagttata gaaggtatgc aaaaatacgg agcagtttat cttgctgcaa taggaggagc
    10441 tggagcatat atttcaaatt caataaaatc atgtgaaatt attgcatatg aagatttagg
    10501 agctgaagct ataagaaaga ttgtagtaga agaccttaag cttacagtag caatagatag
    10561 ctatggaaat aatatatatg agcaaggtcg agcaatttat gaatgtaaat aatgtttaga
    10621 aataaatatg aatatcaaaa ttaaaaatag ttgtgtatat tttaaggagg aaaattgatg
    10681 tctaaggatt atgcaaaatt ggcattagaa atgcatgaaa tcaacaaagg gaaagtaagt
    10741 gtagaaagca aggtagaagt taaaactaag gatgatttat caacagcata tacaccaggg
    10801 gttgctgaac cttgtttaaa aatacatgaa aatcaagatg atgtttatag atacacttct
    10861 aaagggaatc tagtagcagt agttagtgat ggaagtgctg tacttggatt aggtaacatt
    10921 ggtgctgaag catctatacc tgttatggaa ggtaaagcta tattatttaa acaatttgca
    10981 gatgtagacg cttttccaat atgtttaaag actaatgatg tagatgagat agttaaatct
    11041 gtagagttga tggagcctgt atttggaggt ataaatttgg aagacatatc ttctccaaga
    11101 tgttttgaga tagaagagag attaaaaaaa tctttaagta tacctgtttt tcatgatgac
    11161 caacatggga cagcaataat agttgcggca gccataataa actcaataaa gttaattgaa
    11221 aataaaaaaa tagaagattt agaaatagtt ataaacggag caggagctgc tggtatagcc
    11281 atagcaaaga tactacttaa tatgaatgtt aaaaatataa tcttatgtga tagaagtgga
    11341 gcattggaag ctagtataga aaatctaaac tatgttcaaa aagaaatgtt aaaagtaaca
    11401 aatataagaa atgaaaaagg acctttaaaa gatattataa aaggaaaaga tgtatttatt
    11461 ggtgtttcag gtccaggagc tgttacaaag gaaatggttg aaagtatgtc tgagaagcca
    11521 ataatattgg caatggcaaa tcctactcca gagataatgc cagaagaagc aaaattaggt
    11581 ggagcattta taatgggaac aggtcgttca gacttcccta atcaagtcaa taatgtactg
    11641 gcttttcctg gaatatttag aggtgcactt gatgttaagg ctaaagagat aaatgaagaa
    11701 atgaagatag ctgctgcata tgcaattgct aatacaataa gtgatgaaga aatatgccca
    11761 gagtatatat taccagatgt atttaataag aatgtagtaa aaaatgttgc taaggcagtt
    11821 aaagaagcag cgataaaaac caaagtaaat agaatataaa tttttatatg ttcatatatc
    11881 cttatattat cgggtatatg ggcattttta ttataggttt atactcatat atccttactt
    11941 ttatatacat atctttatga ttaaaggtgt tagaaaatgt agtaaatttt atactatcag
    12001 actctatctt tattacttta gtagatacat ggttgtaaag gtttatagaa aaaaatatag
    12061 gaaggcagat ttttattgtt gcgtatatat gagaatgata taatatgaat attataaatt
    12121 aataaattta aatttatgga ggtacaagag atgatggaat ataaagttaa tgaccaagaa
    12181 cttaatgctt caatgtttat cccattcgtc aacaggatat ggccaggaga ttatgatatg
    12241 gataaaacac agactgcatt atcaaaaact ttgaatataa ctgcttatga tgataaagtg
    12301 cttgtaggat gtctgcgagt tctttcagat ggttattatt ttggaacaat tacagaattg
    12361 cttgttcttc cagagtatca gaaacaaggc attggtagta agcttttcca gcttgctaaa
    12421 gagaacaccc caactatgct atattttggt gcacagccag gagtagaaga gttctatgaa
    12481 aaaaatgggt gccaaagaag tctgcaatcc tacataataa aaaagaaaga tagttattaa
    12541 agttccagct ttcaataatc ttaaaagtac aatattttag tggaagcatt tcatttttta
    12601 ttactaatat gacataaaca tgtcatatta gcattgatat acttttaata tggttaagag
    12661 ccaaagaaaa atataaggag tgatttaaaa tggaagaaac taaattttgc caatgctgtg
    12721 caatgccaat gggacaaact gatgaattat atgggacaaa taaagatgga agtaaaagta
    12781 ctgattattg tagttattgt tttaaagatg gtgagtttac agctgatata agtatggaag
    12841 agatgataga agtttgtatc cctcatatgc ttcaaagtaa taaggatatg actgaagatg
    12901 aagcaagaac aatgatgaac aatttctttc caacattaaa acgctggaaa taattttatg
    12961 caaaattagt tatatgcaaa acatgaaata tggtgttgag aataaggggt ggtgtgcttt
    13021 atagcatacc acctgttttg ttacttggag ttcagttatt ttcctaaata ttttaaatat
    13081 actattttag ataagttccc tcttcatctt gataaattaa tccttttttc ataagcactc
    13141 caagagctcg tttaaaattt ttcttacttg aattaaataa tatagaaata tctttaggat
    13201 cagatttatc attaaatctc atatatccat cagaaccttc taaataactt aaaatcttat
    13261 tttctaaagt atcaagttca tcctttctat ttcctctagg agaaagacca agttttccat
    13321 cttcatatat ttttatgaca tgtaaatttt caagaacatc accagttttt agttcagtaa
    13381 agtactcatt atgaagtatt acaccagcat atttattgtc tacacatatc attgcagaat
    13441 tattagtttg gaatccatat acaactccag ttacaatatc tcctacattg taagtatggt
    13501 ctgttaataa atatgaatct atatctgtag tagcagcaat tcttttgctt ttgtctaaat
    13561 atatataaaa taaatatttt tcgtctctaa atagtggata agtcttagct ttaaaaggaa
    13621 ctaatatgtc tttaggaagt cccatatcta taaaagtacc aatgtctgta ttatcaacta
    13681 cttttaaatg tgctacatcc ccaaccttag ctaaaggcgg tattaaagtt gcagcagttc
    13741 tgtcatcaga atctttaaat atgaaagcat ttacttcatc acctatgttt atttcatttt
    13801 ttccgattaa tctattatga agaagtatat cgtcttttgt gttattagtt tgtccatcca
    13861 aaaaatatcc aaattcagtt tttcttttta ctgtaagttt attaaaatca cctatgttta
    13921 tcaaattaaa atcatcctct cattttatat ttataattta gccataatat acttatgtag
    13981 tagtttgtca cagtatctat attagtatta gtgaaattta caataaataa tatagttatt
    14041 tatgtatttt tcaattctct agcttcttat atagtataaa ctccaatttc ttttttgtct
    14101 ttaggtttta ttaaaatttt tacaaaataa gttaaaaagt attgaaataa aatttctttt
    14161 aaagtataat tggtatatac caaaattggt atataccata tcgaaaagtg gtgactatat
    14221 gaaaatagta aataaatcta gtaatatacc attgcatact caattaagtt caataataag
    14281 agagatgata gaaactggtg agcttaaaga gggtgatgca ataatgccag aaagagaact
    14341 ttgcaacata caaaatgtaa gtcgtatgac agtaaataaa accattgttg gattagttac
    14401 agaaggactt ctttataggg tacaaggtaa agggacattc gttgcaaagc aaaaaaagaa
    14461 gtatcagttt tcaaatgtaa aaggttttac tgatgttatg aaagaaaagg gagtaaacat
    14521 aaaaacggat atattatcct ttgagatgga actaccagat gatttagtta aaagaaagct
    14581 tggaataagt gataatacta caaatatata taagattgtc agacttagat atactgatgg
    14641 agagccattt ggattagaaa tagtatatct atcagaagaa atgtgtaagg gtctaacaaa
    14701 agggatattg gataattctt cattatatag ggttttaaat gaaaaatatg gctataagat
    14761 acaaaaagct gaacaggtaa tggagccagt aatactttca gatgaagaaa gtaagcttct
    14821 tgaaacagat gaaggtgctt tagctcttaa acttcataga aattcctata atagagaagg
    14881 tagccctata gaatatacca tatcaatatt tagaacagat aaatatcaat atgaaatagt
    14941 attgagtgag taggtgaata atatgaaatg tttaattaat ggaaagataa tattaaaaaa
    15001 tcaaatctta gaaaataaag tattagtatt tgatgaaaaa ataattgata tagctgatag
    15061 tgtaccaaaa gattgtgaag ttatagatgc tgatggaaaa tatatatctc cagggcttat
    15121 tgatattcat atacatggaa acatgggaaa agatacaatg gattcgactg atgaatctat
    15181 tgaaacaatt tcaaagtcaa ttatgagaca tggagtaaca tcttttcttc caactacaat
    15241 gactatggat aaagaacatg tttatgatgc acttgaagtt attaaaaaag cccaaaatag
    15301 aaagcttgaa ggggcacaag tattaggagc acatttagaa ggacctttta taaatgagaa
    15361 ttataaggga gcacaaaatg aaaaatttat aataaattca aaatacgaat ttataaaaga
    15421 atataaagat gttataaaag ttataactta tgctccagaa aaagatattg attttgattt
    15481 tactagagaa ataaaaagat gtacagatat agttttatct attggtcata gtaatgcaaa
    15541 ttatgaccaa gctaaagaag ctataaattt aggagtaact aatgtaactc acatgttcaa
    15601 tgcaatgact ggtttaaacc atagagaccc aggcgttgtt ggtgcagcac ttacaactaa
    15661 tgtgtacagt gagttaatag cagatacaat ccatataaat aaagatttat ttcaatttat
    15721 actaaataat aaaggaaaag aaagactgat tttaataaca gattctatag aagctggtgg
    15781 tcttgaagat ggcaattatt ctcttggagg tcagaaagtc atagttaagg gtaatgaagc
    15841 tagacttgaa aatggagcat tagctggaag tgtgttgtct ttaaataaaa tggtatttaa
    15901 cttcttagat aatacaaacc ttaaagtgaa tgaagcaatt aatttagctt ctttaaatcc
    15961 agcaacatct ttgggaataa atgacaaaaa aggaagctta gaaataggta aggatgcaga
    16021 tatagctgta tttgatgaga atttagattg taaaatgaca ctttgcttgg gggacgtagt
    16081 atataaaaat atatagtata caaaacaaac cttaaagaca gtaaaaacaa tataaaaaat
    16141 aataaattaa acttacatta gtaaaagtag tagttaaaat ttaggaggaa taagatgaga
    16201 gtattagtgt gtaaagatta tgatggaatg agtaaaaaag ccgctgaaat gatagcagct
    16261 caaatagttt taaaaccaaa ttcaatactg ggactagcta caggaagtac accagtaggt
    16321 atgtatagag atttagtaaa aaaatacaat gataatatag tagatttttc agatgtgatg
    16381 agctttaatt tagatgaata ttataagtta ccaatatcta atgaccaaag ctatgattat
    16441 tttatgaaag agaatctatt taatcatgta aacataaagc cagaaaatac tcatctacca
    16501 aatggaatgg ctgatgatat tgaaaaagag tgtatgaact atgaagcttc tatagatgca
    16561 gctggtggta tagatgtaca agtacttgga ataggtcgta atgctcatat aggttttaat
    16621 gaacctgata ctaaatttgc aaagagaact catgtagttg aacttacaga gtctacaata
    16681 gaagctaatg caagattctt taaatcaaga gaagatgttc ctaaaaaagc tgtaagtatg
    16741 ggaataggtt caatattgaa gtctaagaaa atattgcttt tagcttcagg agaagaaaaa
    16801 gctgatgcag tatataatac tgtttatgga gatataactc cagaagttcc tggttctata
    16861 cttcaacttc ataaagacac tatagttata gtagatgaag cagcagcaag taaattgaat
    16921 ccaaaagatt ataaattagt ataagaatag aatgtagttt aagtaaaaaa tagctatcat
    16981 tgagtaatca aatttattta atgatagcta tttttattta aattttttac ttctttttat
    17041 ttaaattagt tattattgtt taaattactt gcttattact ttttacgaaa actcgttatc
    17101 aagaagtatc caaataagat gttaaaaatt attatgttta gtgggattct atcaagcata
    17161 ataaaattac ctataattgg gatattacca actggaaaca taaagaagaa tacaggcatc
    17221 agtgatagta aaaaagatgg aaaacaaaga agaagtaatc ttggaatatc aatataccaa
    17281 ctaccatcct ttttgtattc tctaatcaat aattcagttc ctagtaataa accaatacca
    17341 ccagaggaaa ttaaattaat aaaaatgtat cttttaaagt cttgcatata accagtgtaa
    17401 aattgagtat agaaatcata atttaattta acggctaaaa aatacataag caatagaatg
    17461 attaaaaata aaagatatct aagagctttt ttcattttaa attctccctt cagaaatact
    17521 ttatttagtt ttattttgtt ggaatctgat tttatcattt agtttaagaa atccataata
    17581 ttagtgaaga atgaaataag tattatatat gaataaatat ttttagaatt aaggtgaatt
    17641 gtagtgctta atataactat aatattaatt gtttgaatat tcaataaaaa gtttgaaaaa
    17701 tatcaattct gatataaact gaataaatac agttgaatat taaattatta ttttatcaaa
    17761 aatatgcaaa gtactacacc ttcaaatatc acctaaagta attaaaaggc aattaaggtg
    17821 aagtagttca agtatttcta aaataagttt aataaattct ataataattt attttttgcc
    17881 ttaaaattgc cttaaaatcc aaacaatttt aaaactaatg tactataaaa agctggtata
    17941 atttaaatat agtaaaaaat aagatttatc ttatctattg ggatttaatt atttaatcaa
    18001 tatatttata ccatttaatt taaataaaaa gtctattaag gagtgagctt atgagtaagt
    18061 taatttatat agcagatgat gaagataata taagaaattt ggtaaagaca ttcttaaaaa
    18121 atgaaggtca tgatgttatg gattttaaaa cgggtgatga acttttagaa caatttaata
    18181 taaaggagtg tgacttagta atactagata taatgatgcc tggctcaagt ggttttgaag
    18241 tatgtacaaa acttcgtgag aaaagtacag tcccaataat catgcttact gcaagagata
    18301 cagatataga ttatattaca gggataacct taggtagtga tgattatttt acaaaaccat
    18361 ttagcccaat gtctttggtt atgagagtaa aatctatatt tagaaggata gagtttgaaa
    18421 agaaacaaaa ttatgataaa tatagtaact cgatagatat ggaattaaaa tttggtgatg
    18481 taataataaa taaaaagaat aaaatcgtta caagtaaaaa tgtaaatata gaccttacac
    18541 caaatgaata taacctattg acttatttgt ttgaaaatat agatagagca gtttccagag
    18601 atgaattatt aaacaaaata tggggatacg atatagaagt tgagacaagg gctgctgatg
    18661 atacagtgaa gagattacgt aaaaaaattt taaatacaaa tattttaata gaaactgtat
    18721 gggggtttgg atttaggttg aaggagaaaa gttaagatga agaatggaaa tgacaagaat
    18781 agaattggaa agattaaatc caaatttaaa ttaaagttct caaagaaaaa tataagcata
    18841 aaaactagga ttttgttgag tatactttgt ttgataatta tagtatttat gataatatta
    18901 gtatctttta atgtatttct tgatacatat ataaaaacaa ctgcgaatga agaattgaca
    18961 aagtctacag aaatagtaga acatatggat agtaatttta gacctattaa gccaccacaa
    19021 gatgatggaa agcttcctcc caaagtatta tctaatttta tgagaaatgt acaagataaa
    19081 gttaggatgg cagaaacaca atcagatgct gatgctatgg tagttgattc taagtataac
    19141 ctgatatttc caacaagaga ggatgatttc ttaaaaaatg ttgatgagat ggagctgatt
    19201 agggagtgta ttcaaaatga aaaattagat ttaaattcag atgaaaatac acggatatct
    19261 accagaaata aggattatta tatttcaact gtaaaaatta ctagtatata tggtgaacaa
    19321 gacaagtatc ttatactttt tatagacata tcaaaaactc taaatctagc acaaaagata
    19381 aatattgtat taatatctgt aatgtgtttt gctggaatat tagcaatatt tacagctgtg
    19441 attttatctg agaaaattgc taaaccgata aaagagttgt gtgagtttgc aaaaaaaatg
    19501 ggtcaaggag atttcaaaag aacctacttt gacttttcag ataaagaatt agtagagctc
    19561 tcaactgtta tgaataagag tgctgagtat ctggataagt atgataatga acaaaaaata
    19621 ttttttcaaa atgcatctca tgaattaagg acaccactta tgtctattaa aggttatgca
    19681 gaagctataa aatataatgt tatagatagt aaacatgcaa gtgatataat tcttgaagaa
    19741 agtgataggt taagtgatat ggttgaagat ttattataca tttctaaaat agataatata
    19801 actaaagatt ttgaacttgt agaatgtgat ttaagagaag tactttcaaa ttgtggagct
    19861 aggcaaaatg taagagctat aaataagggg ataaaattta tttatgaatt tgatgaagaa
    19921 atggttttat ttgaatgtga tgaaaaaaac atttccaaag catttatgaa tcttatagaa
    19981 aatgctttga gatacgctaa gagtgaaata aaaatagtgt gtaagtataa tcaaaaaaat
    20041 atagttgtga taatagaaga tgatggaatt ggaatcaaga aagaagattt acctcatgtt
    20101 tttgaaagat tctataaggg agtaggagga aatcatggaa ttggattatc aatagttaaa
    20161 tctattgtaa ataagcatgg tgggcgtata tacgtagaaa atggcaaaaa aggagctaaa
    20221 tttactatat tatttaaatt gtaaaaaaat tagagaaaat atagagatgt ttgaagttgt
    20281 tttttatata tatctgttgt atactgttga aaaaagaaca atattaagct gacatatatt
    20341 acgatagttg acatttaaag taaatgctta aatatagata cgaaaggaaa agtaaaatct
    20401 gaagtgtatt taggtttaag taagcttctt attttataaa aaatatgtat gaaagaacag
    20461 ctgattttta atattacaaa tttagtaacg gttgtatttg aagcttttgt catccatatg
    20521 tttttatcag actttttagg tcagaaagaa gggtatataa aagttgtaag atatgctaaa
    20581 ctgggtttta taatatgttt aggattctgt aatttaataa ctttaaaccc taaaatcaca
    20641 atgcctttaa ttttcatcct tattttttca acatcatttt tatataaagg aaatttaaag
    20701 acacgattgt ttactactgt tttattaagt atattcttta tactgtcaga aattgtagtt
    20761 acaagtattt ttgttttatt tgtaaaagaa ggctttgaaa taatgcttga aaataatagt
    20821 ataagagtat tggcgacaat tttatctaaa attgtctttt tgttgacttg taaaattatt
    20881 tgtctattta aaaaggatgt acatttagat atgcccataa agtattggct ccctttattt
    20941 ttaataccta tatttagttt atttctgtct gtttctatct ttgatgtaag taaatttttt
    21001 agtcttgaaa gtttaaaatt tctaagctta atatctagtg ttggtatttt atatattaat
    21061 tttattgtct tttatttatt taaatttatt atagataaaa ctaaactatc tatgaaatat
    21121 gaactacttg aaaaagaaat aattcataag gaagaattaa gacttagtaa tgagtgttat
    21181 aaaattattg ttgaacagac tgattctgtc gttttcgaat ggaatatcaa agaaaataaa
    21241 tcatttgtat cgcaggcttg gactgaaaaa tttggatata acaatgcatg taagaatatt
    21301 tttaaagaaa ttaaagataa agacttagtt cattctgaag ataaagctat atttgaaggt
    21361 tttcttgaaa gtattaaaaa aaagaatatg cacaatcaag cagtatatcg tttaaaaaaa
    21421 tctaatggtg aatatatttg gtgtagaaca tcaattacaa gcatttataa tgatgaaaat
    21481 gaacttttaa gagttgtcgg agtaattgta gatgtagata gtgatataaa aaaatatgaa
    21541 gagttaagaa ctagagcaga aagtgactca ttgaccaata tatacaataa aggtacattt
    21601 gaaaaattag tggaagaaac tatagttatg aatacaggtg ataaaaaaga tgcattattc
    21661 attattgatt tggatgattt taaagaaata aatgataatt ttggtcatcc atttggtgat
    21721 tttgtactaa aaacatttgc tgataagata caaactagtt ttggtagtaa agacttagtc
    21781 ggaagaattg gtggagatga atttgttgta tatatgcaag attatgttac agaagtaaat
    21841 ttgcataaaa aagctaaaga attaaataga gtactttcgg ataattacac tgatttgagt
    21901 tttagttttg atgcttctgt cagtataggt attgcaaggt atcctcaaga tggaacttca
    21961 ttttttgaat tgtttaaaaa tgcagataga gcactttata gcataaaagc atcaggtaaa
    22021 aattcatatt gtttatttga agaagaacta tatgtacagt gaatttgaat ttagatttta
    22081 tgtgagagta agatttatat atacttgtgg ctgtctgatt tataaaatat gttactttgt
    22141 atatttttat atgtttcagt aaggttaatt aacatgagca aaggtgtaag taaatttata
    22201 ttagtttaag tttacttaca cctttataaa aatacaaata atagattcat ttacattctt
    22261 aaatgattaa agctctttac cttctaaaca tgcataacat atcgttataa ttccagtaaa
    22321 taagactaaa atgcccatta ctaagattac aatttcatca tctattactc caaatgtttt
    22381 taaagtaact tatttcgctt tgtatcataa taatttgtct ctttttacat tttaacttct
    22441 gctgagattt gagttagata ttcattagaa tctcgcacac ttatatcata tagaagatat
    22501 tcttcataaa caaaatcccc aatacgtaaa ttattctgat taaaaaaatt taataacttt
    22561 tcataagcct tgtaaatagt ttcataactc cctttatgat aagcagttac ataaagtccc
    22621 tttggtttga ttgatacagg atatttcttg tactctttat taacttttgt aaataaatat
    22681 gaatatgatt caaagtcttt attaattata tcttcacctt taatgattga gccaatcgta
    22741 ccataatcac tcaattcata gttgtccata aagttaatac attcatataa aaaattttct
    22801 tcctcatctc ctaaaaactg ttctttatat gtaacaggtg ttttgaccaa gtactcttct
    22861 tcatgttctt ttaaagtaat agtattcgca tctatattac atgcactttt agtaaatgaa
    22921 atagtagatt ccattagagc ttgaatatta tttagttttt caatttcatt tttaatatca
    22981 atggttttct tttctaaaag ctcaattagt aaattaggtg ttcttttgtc tatataagct
    23041 ttaatttctt ttaaaggcat ttttacttcc ctaagtgaag aaattatacc aaaaatgcta
    23101 agttgatggt atgagtagta tctatatcca ttttctttct ttaattctgg tgagaaaaga
    23161 cctatatcat cataatggaa aagtgttttt ttattgattc cacaaatctt tgcaaattct
    23221 ccagttgtaa aataattttt tgaaacttga ctcataatta taattcctcc ttgactatcc
    23281 tgttacagta tagtttatca tagtattttg taaaggagtg tgattatatg aaaaaattaa
    23341 ttcatttttt aaaaccatat agagttttaa ttgttgtagt tttgatattt acgtttttac
    23401 aaacacttgg aactttgtat ataccaacac ttacagcaaa tatagtaaat aatggtgtag
    23461 taaagggaga tatagattat attgtaaaaa caggtttaat gatgatgata gtagctggta
    23521 taacagcttt atcagctgtg ttagtttgta aagtatcagc aaatctttca tctggatttt
    23581 gtagagatat tagagaagca gtatttataa aatcacaaga tttatcaata aatgatttta
    23641 ataatatagg tacagcatca atgattacta ggagtactag tgatattact ttaataggtc
    23701 agtctgtgtt tatgtttatt caattagtat taccagcacc aattataact gtttctggtc
    23761 tattcttagc atattctata gataaagcaa tgacaattat tattgttgta gtaatgtttt
    23821 tattcatgct ttcagcattt ttagttggta aaaagcttat aaagttgttt aaaatgatgc
    23881 aaataaagat ggataatatg aatagggtgt tacgtgaggt agtaactgga gtaagagtta
    23941 taagagcttt taataggagt cgttttgaaa aaaagagatt tgatagaact gcaatagatt
    24001 attctgaaac agcaatttca ataaataaaa tatttgcagt acttatgcct attgtaatgt
    24061 taataatgaa tttaggtata gtaagtatta tttggtttgg tgggatgaga gtcagtaatg
    24121 gaaatatgga aataggtcat ataatggcac ttgttgaata ttgtatctta attttattct
    24181 atttaatcat gggagtaatg gtatttatgt acatacctag agcaggtgca tgtgcagata
    24241 gagtaaatca gatattagat atagaacctg aaattgtaga tggaaatggt cataaagata
    24301 ctgtctcaga gagaggacat ttagtattta aaaatgtaac cttttcatat gcacaatcag
    24361 aagaacctgt gttaaataat ataacatttg aagcaaaatc tggagaagta actgcaatta
    24421 taggaagcac tggctcaggc aaatctacta ttgcaaatat aataccacgt ttttttgaaa
    24481 ttcaatctgg agaaatatct ataaatggtc aggatattaa gaagattcct caaaaagaac
    24541 ttagagataa gattggattt gtaccacaaa aagctttttt atttagtgga acaatagaag
    24601 aaaatataag gtatggaaaa gaagatgcaa gtattgaaga agtaaagcat gctgcaagta
    24661 tagctcaagc tgatgaattt atttatgata tggaagacaa gtatgactca tttgtagcac
    24721 aaggtggaaa taatttgtca ggtggtcaaa aacagagaat atccattgca agagcgttag
    24781 ttagaaagcc tgaagtatat gtctttgatg atagtttttc tgcattagat tttaaaacag
    24841 ataaaagact tagaaaagcg ttgaaaaatg aaatcaaaga ttcatcagct ataattatag
    24901 cacaacgtat aagtacaatc atggatgcta atcagattat agttttaaat gatggaaaaa
    24961 ttgtaggtat tggaaaacat aaagatttac ttgaaaattg tgaagtttat aagcaaattg
    25021 cggattcaca acttagtaaa gaagaacttg cataaaatga agaattaatc tgatatttgg
    25081 aggtgtatga tatgagtgaa aaaagaatag atgaacaaga tatagaagta cttagttttg
    25141 aagatggcat gtcagcagaa aaagctaagg atgcaaaaaa aactactaag agattattaa
    25201 aatatatggc aaaacaaaag tttaaaatat taattatttt tatttcagta ttgataagtt
    25261 cagcgcttac agttttagca cctatggtta tgggaaaagc tattgaccaa ctatttaatg
    25321 gaataaaaac tgcagtccaa acaggtacaa aattcagtgt gaattttagt actatgggag
    25381 ggatagtatc aatacttcta gggttgtact taattagtgc agtatttata tatattcaac
    25441 agtatattat gtcaggagtt gcacaaaatc tagttcttag catgagaaaa gatttaagtg
    25501 ataaactcaa taaacttcct cttaagtact atgattctca taaaaagggg gaaacactta
    25561 gtatagtaac aaatgattta gaaaaagtag cagatagttt acaagaagga ttaatgcaac
    25621 ttattacagc agtagttaca gtagttggtt ctattgtaat gatgataagt ataagtgtac
    25681 cacttacaat agtatcagct ataacattac ttgtttcatt gggtattact gttgtaattg
    25741 caagaaaatc acaagaaaga ttctctgaaa atcaaaaggc actaggagag ttaaatagca
    25801 atatagaaga gatatttact ggtcaaatag tggttaaagc atttagtaaa gaaaaggata
    25861 ctataagaaa ctttaaaata ttaaatcaaa atctttataa tgcaagtaga aaagcacagt
    25921 tttcgtcata tgcaatatct ccaattatta gatttataaa cctaattggc tatgttatta
    25981 tagcagttgt aggtgggata tttgcatcaa ctggagcaat gactcttggt agtatacaag
    26041 cgtttattca atatgtaaat caagcatcag agcctactac agaaatatct tatattgtaa
    26101 atatgcttca agcagcaata gcatctgcag agagagtttt tacagttatg gacgaggtgg
    26161 aagagataaa ggatgaagaa tcttcaaagg ttattagcat gcctaaaggt aaagtacaat
    26221 ttgagcatgt taaatttgga tatagtgatg attttattct tatgaaagat attaatatca
    26281 atctaaatgc aggagataag attgcaattg ttggacctac aggagctgga aagacaactc
    26341 ttgtaaattt acttatgaga ttttatgaga tacaaggtgg aagaattact attgatggtg
    26401 ttaatataaa agatttaaag cgtggagaac ttagaactat gtttggtatg gttcttcaag
    26461 atacatggct atttaatgga agtataaaag aaaatattgc atatagtaag tcagatgcca
    26521 caatggatga aattgtaaga gcagcaaaat cagcaagagt agaccatttt ataaggactt
    26581 taccacaagg gtatgacact atattgactg aggatgcttc taatctatct caaggtcaaa
    26641 agcaacttct aacaattgcc agggcaatac tttcagaccc atcagtattg attcttgatg
    26701 aagcaacatc tagtgtagat actagaacag aggtagaaat tcaaaaggca atgaataact
    26761 tgatgaaggg aagaactagt tttgttattg cacatagatt atcaactatt cgagatgctg
    26821 atttaatatt agtaatgaaa gaaggaacta ttattgaaca aggaagccat aaggaactta
    26881 ttgaaaagaa aggattctat gaagaacttt ataacagtca atttacaagt gaatatgatg
    26941 aagatgttgt atgattatca agttttaggc aactaaatga ttgataaata ttaagttaaa
    27001 tatagtaaca tgtaaggtta tatggaataa gagtatttct ctatttcata taaccttttt
    27061 tatttatcaa aataagataa aatttccttt ctaaattaga ttgaagaatg tatttatcaa
    27121 tataaaagta aaataatcat tattattagt gaagtaaata ttgtttgcca catatatgcc
    27181 ataaagatgc acttatttta cggagattta acaaagtagt gtaaagtata ctctaaatat
    27241 aaaataaatc aaatagagtt aaataaattg agaggtgata taaatagtga ataaaaaatt
    27301 gaactataga tttgaaatga agcacaaaat aactgaagca gatgtgttag ctttaaaatc
    27361 aagactctat ccaataatga agaaagatga aaatgcatct aaagatggaa aatatcttat
    27421 taggagttta tattttgata ctcctgaaga taaagcactt ttggataaat taaatggcgt
    27481 tgcaataaga gaaaagttta ggataagatt ttataataat gactgctctt atataaggtt
    27541 agagaaaaaa atcaaacatt acaacatgac atcaaagcta tcaactaatt taaccaaaaa
    27601 agaagtaata aatatactaa ataatgatat tgaatttcta aaagagtcga caaattgttt
    27661 attaagagaa ttttatttga aattaaaatg tgaaagatta gaggcaaaaa gcatagttga
    27721 ttacaataga gaagctttta tttatccagt tggaaatgta agagttacaa tcgatagtga
    27781 tattaaaact tctgtaaatt cggtagattt atttaataaa gatttgccaa ctgtttcggt
    27841 aattgatgag aatatgactg tattggaggt taaatatgat gaatttatac cagattttat
    27901 aaaggattta attcaaatta ataagactac ctctacagca gtttcaaagt atgcttcaag
    27961 tagattatat atttaagaat aaaggctatg atttatataa agatatacat aaataagatt
    28021 aaaaaacaaa taagttaaaa ataaaaaata aataagttaa aaataggaag gatgatttta
    28081 attatgacat ttaatgatat ttttaaatca agttttatag aaaacgtatc aggtttttca
    28141 tttgtggaca gtgctttagc actaggttca gcttttttag taggtttgtt tatatatatg
    28201 gtctataaaa agacttatat gggaattatg tattctcgac cattcaatgt gtcgcttgta
    28261 gcacttacaa tgcttacaac ttttgtaata ttggcagtta cttcaaatgt agttttatcc
    28321 ttaggtatgg ttggtgcttt atctatagta agatttagaa cagcaattaa agacccaatg
    28381 gatttagtat ttctattttg gtcattgggt tcaggtatag ttttaggagc aggtttgatt
    28441 cccttagctg tcatgggttc aataattatg ggattgattc taatattctt ttcaaataaa
    28501 acaatatctg aaactccata tatattgatg gtaaattgta ataatgaaga ttcagaagat
    28561 atagcaactg ataaaattaa aaaagtattt actaaatatc aaataaaatc aaaaagtgtt
    28621 acaccagaaa aaggaataga attagtgttt gaagtaagaa tgaaagatgg agaaacaagt
    28681 cttataaatg acttgagtca agtagatgga gttactaatg cagttcttgt aagttataat
    28741 ggtgactatg tagcatagag aggagtaatc ttttatgaaa gataaaaaat ttacccttct
    28801 tatctcgatt atgattatat ttttatgtgc tgtagttgga gtttatagta catctagcaa
    28861 caaaagtgtt gatttatata gtgatgtata tattgaaaaa tattttaaca gagacaaggt
    28921 tatggaagtt aatatagaga tagatgaaag tgacttgaag gatatgaatg aaaatgctat
    28981 aaaagaagaa tttaaggttg caaaagtaac tgtagatgga gatacatatg gaaacgtagg
    29041 tataagaact aaaggaaatt caagccttat atctgtagca aatagtgata gtgatagata
    29101 cagctataag attaattttg ataagtataa tactagtcaa agtatggaag ggcttactca
    29161 attaaatctt aataactgtt actctgaccc atcttatatg agagagtttt taacatatag
    29221 tatttgcgag gaaatgggat tagcgactcc agaatttgca tatgctaaag tctctataaa
    29281 tggcgaatat catggtttgt atttggcagt agaaggatta aaagagtctt atcttgaaaa
    29341 taattttggt aatgtaactg gagacttata taagtcagat gaaggaagct cgttgcaata
    29401 taaaggagat gacccagaaa gttactcaaa cttaatcgtt gaaagtgata aaaagacagc
    29461 tgattggtct aaaattacaa aactattaaa atctttggat acaggtgaag atattgaaaa
    29521 atatcttgat gtagattctg tccttaaaaa tatagcaata aatacagctt tattaaacct
    29581 tgatagctat caagggagtt ttgcccataa ctattattta tatgagcaag atggagtatt
    29641 ttctatgtta ccatgggatt ttaatatgtc atttggtgga tttagtggtt ttggtggagg
    29701 tagtcaatct atagcaattg atgaacctac gacaggtaat ttagaagaca gacctctcat
    29761 atcctcgtta ttaaaaaatg agacatacaa aacaaaatac cataaatatc tggaagagat
    29821 agtaacaaaa tacctagatt cagactattt agagaatatg acaacaaaat tgcatgacat
    29881 gatagcatca tatgtaaaag aagacccaac agcattttat acttatgaag aatttgaaaa
    29941 aaatataaca tcttcaattg aagattctag tgataataag ggatttggta ataaagggtt
    30001 tgacaacaat aactctaata acagtgattc taataataat tctaatagtg aaaataagca
    30061 ctctggaaat caaagtgatg aaaaagaagt taatgctgaa ttaacatcaa gcgtagtcaa
    30121 agctaataca gataatgaaa ataaaaataa aactacaaat gatagtgaaa gtaagaataa
    30181 tacagataaa gataaaagtg gaaatgataa taatcaaaag ctagaaggtc ctatgggtaa
    30241 aggaggtaag tcaataccag gggttttgga agttgcagaa gatatgagta aaactataaa
    30301 atctcaatta agtggagaaa cttcttcgac aaagcaaaac tctggtgatg aaagttcaag
    30361 tggaattaaa ggtagtgaaa agtttgatga ggatatgagt ggtatgccag aaccaccgga
    30421 gggaatggat ggtaaaatgc caccaggaat gggtaatatg gataagggag atatgaatgg
    30481 taaaaatggc aatatgaata tggatagaaa tcaagataat ccaagagaag ctggaggttt
    30541 tggcaataga ggaggaggct ctgtgagtaa aacaacaaca tacttcaaat taattttagg
    30601 tggagcttca atgataataa tgtcgattat gttagttggt gtatcaaggg taaagagaag
    30661 aagatttata aagtcaaaat aagattaaaa ttgaatataa ataattttaa tactgttaag
    30721 tctaaatatt tacttagcag tattttttag aaaataatgt atttggttac taatataagg
    30781 taataaattg ataaaaatta
//
LOCUS       DAELNY010000028        30066 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.028, whole genome shotgun sequence.
ACCESSION   DAELNY010000028 DAELNY010000000
VERSION     DAELNY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.028"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..270
                     /locus_tag="KPE55_001108"
     CDS             <1..270
                     /locus_tag="KPE55_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRSP domain-containing protein"
                     /protein_id="HBG0492204.1"
                     /translation="ENQYDIYYHSTTRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYI
                     YNIDKHNFSKCYLFVELNKSENSYLEFIDIIKKTSIKELTIVCCS"
     gene            324..1412
                     /locus_tag="KPE55_001109"
     CDS             324..1412
                     /locus_tag="KPE55_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG0492205.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1440..2231
                     /locus_tag="KPE55_001110"
     CDS             1440..2231
                     /locus_tag="KPE55_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG0492206.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2346..2900)
                     /locus_tag="KPE55_001111"
     CDS             complement(2346..2900)
                     /locus_tag="KPE55_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0492207.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            3096..4298
                     /locus_tag="KPE55_001112"
     CDS             3096..4298
                     /locus_tag="KPE55_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG0492208.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4355..5335
                     /locus_tag="KPE55_001113"
     CDS             4355..5335
                     /locus_tag="KPE55_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG0492209.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5578..6531
                     /locus_tag="KPE55_001114"
     CDS             5578..6531
                     /locus_tag="KPE55_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0492210.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            6521..7477
                     /locus_tag="KPE55_001115"
     CDS             6521..7477
                     /locus_tag="KPE55_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0492211.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            7474..8229
                     /locus_tag="KPE55_001116"
     CDS             7474..8229
                     /locus_tag="KPE55_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0492212.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            8321..9286
                     /locus_tag="KPE55_001117"
     CDS             8321..9286
                     /locus_tag="KPE55_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0492213.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
     gene            9767..11626
                     /locus_tag="KPE55_001118"
     CDS             9767..11626
                     /locus_tag="KPE55_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0492214.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(11918..12517)
                     /locus_tag="KPE55_001119"
     CDS             complement(11918..12517)
                     /locus_tag="KPE55_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0492215.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(12620..13417)
                     /locus_tag="KPE55_001120"
     CDS             complement(12620..13417)
                     /locus_tag="KPE55_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0492216.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(13530..13636)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(13728..14714)
                     /locus_tag="KPE55_001121"
     CDS             complement(13728..14714)
                     /locus_tag="KPE55_001121"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0492217.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            15198..16928
                     /locus_tag="KPE55_001122"
     CDS             15198..16928
                     /locus_tag="KPE55_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG0492218.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            17038..17118
                     /locus_tag="KPE55_001123"
     tRNA            17038..17118
                     /locus_tag="KPE55_001123"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17072..17074,aa:Leu,seq:tag)
     gene            17453..17968
                     /locus_tag="KPE55_001124"
     CDS             17453..17968
                     /locus_tag="KPE55_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG0492219.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      18460..18564
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            18774..21248
                     /gene="gcvPA"
                     /locus_tag="KPE55_001125"
     CDS             18774..21248
                     /gene="gcvPA"
                     /locus_tag="KPE55_001125"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG0492220.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            21248..22705
                     /gene="gcvPB"
                     /locus_tag="KPE55_001126"
     CDS             21248..22705
                     /gene="gcvPB"
                     /locus_tag="KPE55_001126"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG0492221.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINVLKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            23128..25485
                     /locus_tag="KPE55_001127"
     CDS             23128..25485
                     /locus_tag="KPE55_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG0492222.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDDNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(25571..25888)
                     /locus_tag="KPE55_001128"
     CDS             complement(25571..25888)
                     /locus_tag="KPE55_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG0492223.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(26472..27743)
                     /locus_tag="KPE55_001129"
     CDS             complement(26472..27743)
                     /locus_tag="KPE55_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0492224.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSETFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   28390..29539
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=28390..28418
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT        11496 a         3320 c         4782 g        10468 t
ORIGIN      
        1 gaaaatcaat atgatatata ctatcattca acaacaagaa gtccaattat taatattgat
       61 aagaataatt atcctattaa aagtaaattt atgctaaaga gtttttataa tatagatatt
      121 caaaattata tttacaatat agataaacat aatttttcta agtgttattt atttgtagag
      181 ctgaataaaa gtgaaaattc ttatctagaa tttattgata taattaaaaa aacaagtata
      241 aaagaattaa ctatagtatg ttgtagctaa agtgttttaa caggataaaa tatatcacga
      301 ttttaagata ggagttgagt catatgagag attttagcac gtattctaaa gatgatgtta
      361 tttttttatt aaaagatata tcaaatttaa ttgaagaaga aggaaataaa gaaagagaat
      421 taaaaataca aaatggtaga cattattcag aaatgatacc aatagagtat gaagttagtg
      481 aagattatct gaatttatat catgagaaac taagagaaaa taaagataaa cttgcatttt
      541 ctataggagt tatgtgtgaa aaaataatta aaaaaaatgg aaatgatgta gtactagttt
      601 cacttgctag agcaggaaca cctattggta ttttggcaaa aagatatata agaagcaagt
      661 ataatataag tttacctcac tatacaatct ctataataag agataaggga atagatatga
      721 atgcaattga atatatagta aagaatcatc cgagctcaaa aattcaattt ttagatggat
      781 ggactggtaa gggaactata tcaaaagaac ttgaaaaagc atgtgatgaa cttaatgtta
      841 aatttaaaaa atattttgac tcaacacttg ctgtattagc tgaccctgct ggatattcag
      901 gactttatgg aattagagaa gatttcttaa taccaagtgc atgtttaaat tcaacagttt
      961 ctggtttagt aagtagaact gtactaagag aggatttaat tggtaaaaat gacttccatg
     1021 gagctaagtt ttatagacat ttaaaagata aagatgaatc tatgaactat atcgaaacta
     1081 tagaagaatg ttttaaaaat caatttaaga atatatctga tgaagttgaa aactgggaaa
     1141 gtgacattat aacaagagat ggatactttg atgttttaaa tataaaagaa aagtataaca
     1201 taacagatat taactttatc aaaccaggtg taggggaaac gacaagggtt ttacttagaa
     1261 gagttccgta taagattctt gttaaagact taaatgataa atctttagac catatattca
     1321 tattagctaa ggagaaaaat gttgaagttg aacaaatgga tttaaaagcc tataaatgtt
     1381 gtggaataat aaaaaatatg aaagatattt aggaaatata ttagacagga gaatattgaa
     1441 tgattgtttt ttcagattta gacaggagta tcatttactc caataaattt ttaaatgctg
     1501 atagtaaata tactaatata gaaatttata gggaaaaaga aatatcttat atatctttag
     1561 atactataaa tttaataaag caaatacaat attatggtat gtttatacct acaactacta
     1621 gaacagtgga gcaatttaag cgtatagaat ttaataaata tgggatttat tttccttgga
     1681 gtataacttc aaatggagga gttatactta aagataatga aatattgaaa tcatggagtg
     1741 aaaaaataga taaactaaaa tgtgattatg agcccattga aagtatgatt cataaattta
     1801 aagactattt gaatgtagat ggaataacta attttaaagt tgcagaagat acattttttt
     1861 atatagttgt tgatttatca agatttaatt tggattcaat caaagaatat acaaatatat
     1921 tggaatcaaa aaattggaag ttttatgtga gtggcagaaa aatatatttt ataccaaaag
     1981 aaatttcaaa agaaaatgct ataaaatact tgactaaaga attaggaata gaatattttt
     2041 atgcagttgg agattctatt atggactatg gtatgcttaa tatctcaaat aaatcctatg
     2101 tattgaagca tggtgatata aataaaaatg aaatagaaaa ttcatttata tcaagttctt
     2161 tcaatggaat gagtggaact gaagagatac tatctaacat tttagacgag aactgcctac
     2221 ttaatatata atgactgata tataaataat aaactagaaa aatctgaaat aaattatagt
     2281 aaagtttatc atactaattt atttcagatt tttatcaatt tatatttaag taagaactca
     2341 atatgttaaa atgaaggtgg agtcattgcc cagactgaga tacatacctc atcatcataa
     2401 ttttcataca catgtggtat agtactgtta aaataaaaac tatctccttc ctccaataga
     2461 tattccttat ttccaagtgt tatcttcatt ttacctttta ttatatagcc acattcttct
     2521 ccttcgtgtg tcacaaattc actgtttttt tgtgtatgac catctagtgt aacaagtata
     2581 aattctattt ttttcccaga taaattagga acaagaagtt cataaaattt tgtagaatca
     2641 ttagttacta ttttttttcg attatttttc tttactacta tatcagagtc ttctcttcca
     2701 atctcttcaa aaaaataacc tatatttata tcaagagctt ttgatatttt ccacattgct
     2761 acaatagatg gaacaaccat attacgctca atttgactta ttaagcctgt acttaaatct
     2821 gaattttttg ctaaagtaga gatacttata tttttagatt ttctaagaga tctaattttg
     2881 cttccaacat ctatattcat tagatacaaa ccccctcaga catgattata acatataatt
     2941 tataaagatt aataaaataa aaaaaaagtt ttacaataca agtatttttt atgctatatt
     3001 gaattttctg aaaagatgca ttataatagt attaatattc aatatattga atattgataa
     3061 cgatatttta atttaatgaa atggaggtaa ttaagatgaa gagttttgta agtcctgata
     3121 atatttgggt attatgggca gttgtaactg gatgggcagc atttagtatt tatttggaac
     3181 aaaagtacaa ttgggcatca aaagtgtcgg gagcaataat agctcttgta ggagcaatgt
     3241 tattatcaaa tctaaatatt ataccagtag agtctgttgt atatgaccaa gtatggggat
     3301 atgttgttcc tttagccata gctttattgt tatatcaatg taacattaaa aagatatgga
     3361 aagaaagtgg aagattgctc attatatttc ttgtaggttc tgtaggaact gtacttggag
     3421 ctatgattgg atttttagca ttaaaaaatg tagtaccaga tttaaatatt gttgcagcta
     3481 tgatgacagg ttcttatata ggtggaaatg taaactttgc tgctatgtca ggggcgtttg
     3541 atgcgccagg agaattagta tcagcaacag ttgtagcaga taatctttta atggcattat
     3601 atttctttgt cttaatagct ataccatcaa ttggattttt tagaaaacat tttaaacatc
     3661 cacatgtaga tgaaatggaa agtataggta taactgaagg agaaacagta gcagcaaatt
     3721 attggggaag aaaagaaata tctttaaaag atatagcaat agccattggt agcgccttta
     3781 taattgttgc agtatctgta gaactatcaa cttggtttaa agctataata ccattatcaa
     3841 atcctttttt agcaatgtta aatactttat ttggaaatca atatctaata ataactactt
     3901 tgactatgtt gtttgccaca tttatgccta atttctttgg tgatataaaa ggagcacagg
     3961 aattgggtac atttttaata tatatattct ttgttgtagt aggtgttcct gcttcaataa
     4021 cattaataat tcaaaaatca ccattactac ttttatactg cggaataatg gtactaataa
     4081 acatgctagt tacatttata gttgcaaaaa tatttaagtt tagtttagaa gaagctattc
     4141 ttgcatctaa tgcaaatatt ggtggtccta caacagcagc agctatggca atatcaaaag
     4201 gatggtctaa gctagtaggg ccaatactta tagttggaac atttggatac atagtcggta
     4261 attattttgg tcttttagta ggaaatattt tgatataatt ttacgtgaaa aaaagttgtt
     4321 tattagaaag atttttattt aattggaggt tgttatgatt ttcgatggac attcagatac
     4381 ttgggcagat gtaacaataa gaaggcttaa cggggaaaaa gatgtattta gaagaatcca
     4441 tcttgataat tttaaaagag caaatataga aggagctatt tttgtaatat ggatagaccc
     4501 accatatgat aaagagcctg aaaaaagagt taaacaaatt ataagttcta taaaagatga
     4561 aattgaagat atgaaagatg ttgtaaacat agtaaaaaaa tattcagata tagataaagg
     4621 tataagtgaa aacaaaataa atattttaat tggtcttgaa ggattaagcc atattggaag
     4681 aaatatagat ttactagatt atttttataa agaagttggt gcaagacatg catcattaac
     4741 ttggaatgaa cagaatgaac ttgctacagg atgtttaggg gatgaaaatc gtggtttaag
     4801 tgaaataggc aaaaaagcag tcaataaaat ggaaaaattg ggcatgatag tagatgtgtc
     4861 acatttaaat gaaaaatcat tttgggattt aatgtctgtt gcaagtaagc cagttatagc
     4921 ttctcattct aatgctagga gtttatgtga tgctaaaaga aatcttacag ataatcaatt
     4981 aaaagaaatt tcaaatacag gtggattagt tggaataaat agttttagag gatttactaa
     5041 tagtaatcct aagaagcaag atttaataca tttggttgac catatagact atatggtaaa
     5101 tcttataggt atagaacatg tatcttttgg ttttgatttt agtgattact tagggaaaga
     5161 gactataggt tcttttactg gaaatgctag tgtgccaagt gtagatggtc ttgaaagcac
     5221 acatacagca cagaacatag tagtagagct tgaaaataga ggttataaaa agaaagatat
     5281 agagttaatc tctagtggta atttttatag gctctttaaa gatatactaa aataatataa
     5341 taaagaactt aaatttataa aatagtatat ttatacaaat aatatctaca tatttaaatt
     5401 ggcgttcata atttattaaa ttttaatgtt actagaagtt tcaattctag taacattttt
     5461 tatgttaaaa acagatatag atatgtattg acaatgataa ttattattga taaactataa
     5521 aagtgaaaat gataataatt ttcattttaa ttaataaaag atttttaagg agggagaatg
     5581 aagaaaaggt atttagtcat aggaataata attttgtctt atttttcttt atttatagga
     5641 gctgaagata taaatataat gcatatattt gccaaaaatc agcataaact tatgatattt
     5701 atcatgagta gaataccaag attaataagt atattgattg ctggtgtagg aatgagcata
     5761 gcaggtctta taatgcaaca gataagtaag aataaatttg tatcacctac aacaggtgct
     5821 acaatagatg cagcacaatt tgggatagta atatgtatgc ttttagtacc aactgcctct
     5881 atttttacaa agacaataat agcatttgta ttttctcttg tagggacttt tacgtttatg
     5941 aaaataatag ggaagttaca atttaaaaat ataatctttg taccattagt aggtataatg
     6001 tttggaaata taatagggtc tatgacggat tttatcgcgt ataaatacga tttgagtcaa
     6061 aatgttagtt cttggatgca aggtgacttt tctatgatac ttaaaggaaa ttatgagata
     6121 ttatatatca caatacccct tataatatta gcatatattt atgcaaataa atttactgtt
     6181 gttggtatgg gtatggattt tgcgacaaac ttaggtctta gttacaaaag aattgtcaat
     6241 ataggtctga taatagtagc acttgttaca gtatgtgtag tcgttacagc tggtaatatc
     6301 ccttttatag gtctaatagt tccaaatatc gtatccttgt acatgggtga taatataaga
     6361 gcaagcatat ggtatactgg tttacttgga gcgatatttg tacttatatg tgatattttt
     6421 ggaagaatta ttatttatcc atatgaaata tctatagggc ttacagttgg agttatagga
     6481 agtatactat tcctttattt aatattaagg aggaatgtga atgaagctta gtctcaatca
     6541 aaaaatttac tttgtaagta tattactatt catatcaatg gcacttttta taatttatgg
     6601 aatagacatg aatcatttag aatatgcatt atcacaaaga ataccaaaaa tatttgctat
     6661 ggtattaggt ggaggatgta tagcatttac tactgtagtg tttcaaacaa ttacaaacaa
     6721 tcaaatatta acaccaagtg tactaggttt agattcattg tatgtaatga ttcaaactat
     6781 aatagtattc atatttggtt caagcagtag tctgattata aatgaaaatt ataattttat
     6841 tattaatgta gctatgatga ttggagcttc attgttatta tataagacgt tatttgaaaa
     6901 aaataaaaat aatattttct ttttaatcct agtaggaatg atatttggaa ccttatttaa
     6961 aagtgcaaca acatttattc aggtaatgat agaccctaat gaatttttag ctttacaaac
     7021 tagtataatg gcaagtttaa acaatataaa cactaatgta ttgttaatag catttataat
     7081 aattattgcc ataatacctt ttatatatga tgaaataaag tatttagatg tattgtcgtt
     7141 aggtaaagaa caagcaatta atttaggtgt agattttgat aaagcagtta aaaaaatgat
     7201 aatattaata gcaattttag tctcaatatc aacagcactt attggaccta tgacattttt
     7261 aggactttta cttgctaata taacaagaga aatttttaaa acatataagc atacatactt
     7321 gatttcgggt tctatgttga tagggatgat tacattgata ataggtcaat tttttataca
     7381 acatgtattt aagtttgata caactttaag tgtagtaata aattttatag gaggaattta
     7441 ttttattcaa ttactattaa agggggcaaa tagatgatag agataaaaaa tatatttaaa
     7501 agatataaga ataaaaatgt tgtagatgat gtctctttta gtatagaaaa gggaaagata
     7561 acatctttta taggaccaaa tggagctgga aaaagtacag tactatcaat agttacaaga
     7621 cttataggag gtgatggtgg agaagtaata atagaaggaa aaagcttgac taattatagc
     7681 aataaagaat tagcaaaaaa aatagctatt cttaaacaat ctaacaatat aactttaaag
     7741 cttacaataa gagaattagt tggatttgga agatttccat atagtgaagg taatctaact
     7801 aaagaggatg aaaattatat tgatgaagca atagaatata tgaagttgac tgatatacaa
     7861 cataagtatt tagatgaatt gagtggaggt caaagacaaa gagcttatat agcaatggta
     7921 atagctcaag atacagagta catattactt gatgaaccac tgaacaattt ggatatgaac
     7981 cattccgtac agatgatgaa ggtacttaga ggtctttgtg atgaattaga taagactata
     8041 gtattagtaa tgcatgatat aaattttgca tcatgttatt cagataatat agttgcatta
     8101 aaaaatggaa aagttgaaaa agtaggtcgt acagatgaga tagtaaatga aaaagtttta
     8161 gaagatattt atgagatgaa ttttaacatt aagaatataa atggaaatcg aatctgtatt
     8221 tatttttaaa atattgtata tgaaaataaa cttgctatat gattttttat aaagttttta
     8281 aattaaaata aagattaata ggagagagga aaatattgat atgaataaaa aagcagcaat
     8341 agtagcagca gtagctatta taggattagt gacagtgttt gcattaggtg gaagtaaaaa
     8401 gaatgaatca aaaacatcag aaaattcaaa taatactatg aagataactc ataacttggg
     8461 agaaacagat gtaaaattaa atcctaaaaa agtagtggta tttgattatt cggctttaga
     8521 tacaatggat gcattaggtg tagctgagac tttagtagga cttccaaaag cgagtttacc
     8581 agcatcctta gaaaaatata aggatgacaa gtatgcagat ttaggaggat taaaagaacc
     8641 agatttagaa ggtataaagt cggcaaatcc agatttaatt ataataaatg gaagacaaga
     8701 agatttttat gagcaattgt caaaaatagc accaacaata agtacaagta aagatgataa
     8761 gaagtactta gaatctgtta aaaataatat agacaaaata gcaaaaatat ttggagtaga
     8821 agaaaaagca aatcaagaat ttagtaaaat tgaaaagaaa atagaaactt tgaataaaaa
     8881 agtaacagat aaaaatttaa atgctttaac tataatggta aatgaaggta atttaagtgt
     8941 atttggtgaa gaatcaagat ttagtatatt atacaatagt tttggatttg aaaataaaga
     9001 taaaaatata aaagaatcaa gtcatggaca aaatattact tttgaatata tagctaaaca
     9061 aaatccagaa gttatgttcg ttatagatag aggaatagct actggcagtg atgtaaaaga
     9121 aagctcaact gcaaaatctg ttttgaacaa tgatataata aaatctatgg atgcatataa
     9181 gaatgataat ataatatatt tagattcacc aacatggtat gtaaatgatg gtggtctaac
     9241 atctttaaat aaaatgatag atgatgcttc aaaagcggta aattaataat ccgatataac
     9301 caaaatgtat tttttataaa atgattatca aataaactag gagtttttaa gaactcctag
     9361 tttatttttt gcataaaaaa gatatagttt aaacaataat attatctata ataagataaa
     9421 tctattcaaa atctaataaa atatgaagtt atactagaca aaaagaataa ctcttaaata
     9481 taattaagtt atataataat atataattat aatgatataa ttttaatagg tggtttgtaa
     9541 ttaaaaagtt tataaattat attaatagta ataatctata agaacttata tattagaaaa
     9601 aaatttttgt atatataaag gactctaaag atgaactatg taaaacaagg tttatatatt
     9661 ctaattgtat agaaaaattt attgtaaacg catgtatatt attagaaaaa atagtttgga
     9721 ggtattcaat aaacataaaa aacaatatag gggataaagg tgttttatga agagattttt
     9781 gaggagaatt atcttagttt tatttatttt gttgattttt ataagtttta tttctattaa
     9841 attaattcat aatgttggag attatggaaa attgattaat tatgttggga ttgttcgtgg
     9901 agctagccaa cgtctaacta aactagagat gaatcatcaa ccaaatgatg aattaataga
     9961 atatattgat gaaatcctac aggagttgat tacagggcat ggagattatg gtttagtaat
    10021 cactgattgt aatgaataca atgaggactt acttttatta gaaaaaaagt gggaagattt
    10081 aaatattgaa attaaaaaag taagaatgaa agaacagaat aaccagttat taagtataag
    10141 tgaagagttt ttttctcttg caaatgatac agtgtttaaa atagaaaact tttctaaaga
    10201 aaaaagtaat tacttaatga ctttaataat aattatatct attataggta tattagcatg
    10261 tatcatattg attttacaat atagtaaaaa aatggttaga ctggaaaaat taaatgtaga
    10321 tttaaaaaat attgcatata aagatgaact aacaggagta aatactatag aaaaatttaa
    10381 attggatgca aatcaaaata tttgtataca taaggataag aaatttgctg tattctatat
    10441 tgattttgaa aactttaaat acattaatga tatatttgga tatgattatg gtgacatgat
    10501 actgaaaaga tatgctaatc ttatgatgaa tgacataggg aaatatgaaa tatttgcaag
    10561 agaaattgca gatagatttg ttgcgttaag atgctatgta gataaggaag atttagtggt
    10621 aagacaacga gatgtagaca atgaactaat taatataaca aatgaaatta aaaataaaca
    10681 tagtattact gttgttagtg gaatttgttg tattgaagat gtaaatgaaa aattaagtat
    10741 agatggttta ataaatagag caaattttgc acaaaaaact gttaagaata aaccaggtac
    10801 taattatgct ttttataatg acagtattcg taaaaaaatg attgaagaaa atactatcaa
    10861 aagtagaata catgaagcta ttgaaaaaag agaatttata gtttatttac agcctaaagt
    10921 taatttacat agtcaaaaaa taaattgtgc agaagcttta gttcgttggc ttactccgga
    10981 taaaggtatt atatctccag caatatttat tccagtactt gagaagaatt tttttattgc
    11041 cttagtagat aagtatgttt ttgaggaggt ttgtaaatgg attagaaaga ggttagatga
    11101 aaataaacct tttgttcaga tatcagttaa tgtttcaaga atacaatttt ataacactaa
    11161 atttgtagaa acttattcca atatacaaaa taaatacagg atacctaaaa atactataga
    11221 aattgaattt acagagagtg ttgcttttga gaatcaaaaa catcttttgg aaattataca
    11281 tgatttgcat gaaaatggat ttacttgttc tttagatgat tttggaaaag gttattcgtc
    11341 tttaagtgtt ttaaaagatt taccatttga tgttttaaag cttgattcta tgtttttcaa
    11401 ggctagttta gataaagata aagagaaaat agtaataaaa aatattgtac atatgttaaa
    11461 agaacttaat atcacaactg ttgctgaagg cattgaatgc aaggaacaag ttgaatttct
    11521 tagggatatt gggtgtgatt tagttcaggg atttgtattt tataaaccaa tgccaatatt
    11581 agaatttgaa aaaatattgg ataaagagtt tgtttataat agctaaaatg tactttataa
    11641 aatatgaact aattaagtaa aattattttg tactgaataa atttttactt tatttacaga
    11701 ataatgatta tttacagaaa agcgcgaaaa gtgtattcct ttaagtgtgg gatggatagc
    11761 acaaataggg gagaaaaaat attggccata taagaatgca taataatata aatatttgaa
    11821 aggtcagcat tatataaaaa ttaaaaagaa aataaataat ttttctataa aaaagagact
    11881 atgagaaaat ttactcatag tctcctaaat aatgtattta ttttcttcct gcaacccatt
    11941 ttaatcccca atgaaattca gtatccattt ctctatgacc tttgaagtat ttaactactt
    12001 tttcaacact gaatgtctca tcatttacat tttctatcaa tgctattgaa cacatattat
    12061 agccattttt atgttcatca agtttgacaa ctaagtctga atcttgtttt tgtttgattg
    12121 ttacaattcc atcagcttca gaccagttag ctacaccttc gtatataaaa gcataaataa
    12181 gtattctttt tatatctttt attttattac catttattct taaattttct ccttgtgtat
    12241 tacttcctgt tctgtcatct ccatctaatt gagcaaaagg tggattgtct aaactaccaa
    12301 aggcatttcc taatgcttga acagcacctt ttacaccatt tttcatctca aataggcaac
    12361 ccaaatctaa atctatatta ctgcttctta aagagccaaa aaaacccttt ttttgtactt
    12421 tttgattcca atttaaatta actaagattt cacctaaatc gctattttcc ttttttgtaa
    12481 ggtttatttt ttgtcccttt tttaattcta tagccatagt aatcttcctc cacaaacata
    12541 gatttattta aatggtagca cttaataagt caatattcaa caagattaga ataataatat
    12601 taaaattata ttaaatatac taaaaagtag gaatcacaat acctttgtat ttttcttcaa
    12661 ttaagcttct tgctttatct gatgtaagaa catcttgtag aactttaatc ttttcactgt
    12721 ctttatcttc agttctaaat gctaaaagat ttgcatactc gtcatttata ggagctgtat
    12781 aaattccgtt tttcttggca tcaagaccag caggtacagc ataagtagta tctataaatg
    12841 cagcagtcac atctggaaga gaagatggaa tttgagcaat atctaactct ttgaattgta
    12901 ttccctttgg attttctatt atgtccttag gactaactaa tccatcccct ttaggtaacg
    12961 ttaaaagtcc aatactttca aggtatctaa ggttcttaga acaagaagat gggttattgc
    13021 ttattgcaat tgtatctcct tttttaaatt catcaacaga ttttatctta taagagtaaa
    13081 gtattgctgg acatacatat agttttttac cagccattat tttgtaacct ttggccttaa
    13141 cagcttcttt tagataaggt tcatgttgaa atagatttgc atcaatttca ccattattta
    13201 aagcttcatt tggaagtatg taatcatcaa agtttttaac ttctaaagta tatccttttt
    13261 ctttaataag tggttttaat tcttctaata actctcctcc tggaactaaa gtagcaccaa
    13321 caacaatttt tttatcatct ttagctttag aacatccaac cacagctaaa gttaatacta
    13381 gacataataa caaacataat aattttttga atttcatata aattccctcc tgaatataat
    13441 tgattagtta aaaataaaat accattcttt atcacactgc aaaataaaaa taagcctgag
    13501 tttaaactca ggctttaaaa gctaatttag actcatcttt cagtataata ctgcaggatt
    13561 tagcaccaat gtatatagaa aatatactgg ttgctgggtt tcatagggcc agtcccttca
    13621 ccactctgga taagaagtat caatttttaa ttatgttata aatattatca tgatttttta
    13681 cgtctgtaaa cataatttta taaatatttt aaaatcttta atgtatttta tttaacacct
    13741 aaaattccag aaactagaat atcagtatca gcacctaaga aatagtcatt tatttcaata
    13801 gaatccagag tatttctcaa agcattttct tcatgcctta taccaataag tttattttct
    13861 acaaatgcaa catcacattt tccaaaaaaa tctccaaaaa attttatact agttattatg
    13921 cctttatcaa catttaaatt aaactcaaca tttccaccag aatattttaa ttcatttgaa
    13981 agagtatatt ttggtgaatt accaaaattc caatcccaag tttcgtattt tgatttagta
    14041 agcttttcta tttgttttat atcatgctca ctcaaacaat aattagtatt atcagtattt
    14101 gttttagaaa gataatccat tatagcatct ttaaattcta atatatccat ttttgaatgt
    14161 aaatgttcac ttatattggt tacacgagat tttacagatt ttattccttt tccttcaaat
    14221 tttataggtt taacctttaa tgcttttgag aggtcattta tttcagatga aaataaaaga
    14281 gtcccatgat gcattacttt gtttttgtag ttgtattgag catttccaga aaatttcttg
    14341 ccatctataa gaaggtcatt tcttccagaa aattcggcat tgacatctag tgttttaagt
    14401 aaatctatta tgggctgagt gaatcttctg aaatcactaa aattattatt gttacaagct
    14461 ataaatgtaa aatttatatt tcccaaatca tgaaatacag ctccaccacc agactgtctc
    14521 ctaacgacag gtatagaata ttcttttata tattctaaat ttatttcaga aagtgtgttt
    14581 tggttttttc caacaattat agaaggttca ttcctccata aaataaataa gtcttcatca
    14641 aaatttttta gaagatattc ttccattgct aaattaaaat aagggtttgt tttttcatta
    14701 taaattaata acattaaagt agttcctcca tagaattgat aaaattttta ttatgaatac
    14761 aattaatttt ataattcgac aataatataa aaactccttt aaattagaaa ttttaaaaac
    14821 taccttgcaa agtttaaata aataatatat actcaaaata tgtttaataa aagtgaatag
    14881 aagggtagag gcgcaataat ctatcagtac taataaggag ttaagcacag agatttatta
    14941 ggaaaggaga tattgccgaa gttttataat cgatgcttta agattatatt gctgggggta
    15001 catagaaaat atgtacaact gtcacaggtt tatttgtggt gagctatcat ttaaggaatt
    15061 atattatatt tatttttact attgttgata tattgtaatg tttaagcctt gagtgtttac
    15121 cactcaaggc ttttttagtt ttattgtaac ataataccct gtacagttca caaaattaaa
    15181 aggagaggga gatttatatg aggaacaaaa aaattaatat tatttttctg acgacaatca
    15241 tgttcataat gtcaacagta atggtttttg cagaagaaga tatagacact attgcactag
    15301 ccaatgcaga aaaatttggg attttaactt taataccacc attagttgct ataatacttg
    15361 catttataac taaaaacgta ataatttctt tacttatagg tattctttct ggtagtttta
    15421 taattaaagc tagtggtatt aatgtttttg cgacatttat acaggcattt ttagatttag
    15481 tagacagagc tttagtatct ttagcagacc cttggaatgc agggataata ctacaggttt
    15541 tggcaattgg aggagtaata aatcttgttg ctaaaatggg aggagctaaa gctatagctg
    15601 aagcacttgc taaaagagca aagtcagcaa aagggacaca gcttataaca tggtttttgg
    15661 ggcttttagt attctttgat gattatgcca attcactaat agtaggtcct atgatgagac
    15721 ctgttgcaga taaaatgaaa atatcgagag aaaagctggc atttataata gatgccacag
    15781 cagcaccagt tgctggttta gctataatat ctacatggat aggtcttgag gttggtttaa
    15841 tacatgatgc ttttgaaagt ataagtatag atgtagatgc atttggtata tttttaaata
    15901 caataccatt tagattttac aatatattaa tattagcttt cattgtaata tcagcattat
    15961 tattaaaaga atttggtcca atgagaaaag ctgagataaa atctagaagt agaaaaatta
    16021 gtatagacct tgatgaaggc gtagaagagt tggatgattt agctccaaaa aatggagtga
    16081 aattgagtgt atggaatgct ataatcccaa taggaacatt gataatagtt gcattagcat
    16141 cattttatta tagtggatat acatctataa tgggaggaga cgataaggct ctgatacaac
    16201 tatttacaaa ttctccatat tcttttgaag caataaaaga agcatttagt gcatctgatg
    16261 catctagagc attattccag tctgcacttg ttgcaagttt agttgctatt ataatggctg
    16321 tagtaaaaaa gatttttaca atatcagaag caattgatgt ttggatagat ggaatgaaaa
    16381 gtttagtaat cactggtgta atattaatac ttgcatggtc attaagttca gtaataaaag
    16441 agttaggtac agctaaattt ttaatacacc tattatcagg ttcactacca ccattcttat
    16501 taccaagttt aatatttggt ttaggagcaa taatatcttt tgctactgga acagcttatg
    16561 gaacaatggg tatacttatg ccacttgcaa ttccattagc atactcatta aatcctgata
    16621 tgtcttatgt aatagtaagt actagtgcag ttttaacagg tgcaatattt ggagaccatt
    16681 gttctccaat ttcagataca acaatacttt cttcaatggg agctggctgt aatcatatag
    16741 accatgttaa cactcaaatg ccatatgcta tatttacagc agtaatcact attgtatttg
    16801 ggtatatacc agctggactt ggattgccaa tatatatagt attaccagtg gcaatagctg
    16861 ctatatttgt aggtattcaa ataattggta agaaagttga tgaagcggaa atagaacttg
    16921 tagaataaag atataaataa gtttcacctt ttattatcac tattttgtta gtttttagat
    16981 aattttaaaa agttattgaa aataatggcg aaatatagta aaataaaaga gtgataagcg
    17041 gatgtggcgg aattggcaga cgcactagac ttaggatcta gcgccattgg cgtgggggtt
    17101 cgactccctt catccgcact ttacagtact taattgtact gaaaaaaact ggggttgctg
    17161 gctttcggag tgggctatcc cgtttttatt tttttgtata tataatagat tggcacttta
    17221 gtataaggtg ctttttttat aggcaaaaac agtacattta tttctgtttt attatcattc
    17281 atctattaga atatacttta gttaagaatg tgttaataag cagtgttcac attcttgata
    17341 aaatgtggaa taatatgtat ttttactaag acggaaatga aataattaaa ccattaaata
    17401 atattataag atgatgaata agtagataaa tcaaattttg taggaggata ttatggaaaa
    17461 atatatatgg ttgtttccat tactttttat ttttcatgat atggaagaga taataggttt
    17521 tggaatatgg cttaaaaaaa ataaatcaat gcttgataag aagtatccat ttatcagtaa
    17581 agtttatgaa aattatagta ctgaaggcat ggcatttgct gtgtttgaag aatttatttt
    17641 atgcattatt ttctgtatac ttacagttat aacagagaat cagtatgtat atctactatg
    17701 gttaggtagt tttatagcat ataccttaca tttggtaatt catatagggc agtcaatcat
    17761 aataagaaaa tatatacctt cacttattac cagtatcata tgcttaccaa taagcatttg
    17821 gtgtataagt aagagtattt atatagttga ttgtgaaatg agtacaacaa ttctgtacag
    17881 tataattggc attataattg tagcattgaa tttaaagttt gcacagtcgc tgattggaaa
    17941 atttacaaag tggatgtcaa acatataaat tttagtttat tggacagttc aaggaatcag
    18001 aaattggaat tttatagtat taatatttta tagtattaat ataatgtata aaattttata
    18061 gcaatgtatt tcataagtat aaagtgacta gaattttaag aaatatacaa attctataaa
    18121 aactaaataa tttcaagtat attttaaaaa tcttattttt cacaagttta aaattgtaaa
    18181 cgatttcatc aagtctatga atatactttt aacatgttat agtaaatctc aatataaatt
    18241 tacatttggt tctttgtgtg ttattttaat aataaattaa gtgaaaatgt tattttacat
    18301 taatatataa tatctttgca taagagtgaa taatataaaa gagaaataat tgataataaa
    18361 aacttaacaa aaaaattaat acataaaaaa aaataaatat aatgtgttga catttaattg
    18421 aaatattcat acaataaaat tgaaaataaa tatttccaaa tgagattagc gagagagtcc
    18481 tttatacgaa aaggcgccga agaagaaatt taagaaatgc caatttttag agaagctttc
    18541 aggcaaaaat atcgctaatt gatgggactc tggagagact taagttaaga tactataagt
    18601 caccgaagaa gcaaaataat attaaattat taaatctttc aggtatacaa acagggatat
    18661 ataggctagg attattttcc atgctgatat atcttttttt tatgaaaaag tacaggaaat
    18721 aatatcaaag ttacttaaat acagtaggtc taattttcta gggagggtta tatatgaatg
    18781 aattgaaaag agtatctttg tacaatatac acaaagagct tggagcaaaa cttgttgaat
    18841 ttgctggttg ggaaatgcct ttagaatatg agggtataaa taaagagcat gagaaggtca
    18901 gaaaaagtgc aggtatattt gatgtatctc atatgggaga ggtacagata aagggagcag
    18961 agtcagaaaa atttatacaa aacctagtta caaatgatat aagcacatta aagataaatg
    19021 acattatata tacacctatg tgttatgaaa atggtggagt agttgatgat ttattaatat
    19081 ataaatttgg tgaagaagat tatttattag taataaatgc aggtaatata gataaagatg
    19141 tagcatggat aataaaacaa agtgaaggat acaatgtaga tataaaaaat atatctagtg
    19201 aagtgagtca attagcaatt caaggaccta aagcagaaga aattcttcaa aaaatcacag
    19261 atatagactt aaactcaata aaattttata agtcaattcc atcaacaaaa gtatgtggtt
    19321 gtccttgttt agtttcaaga acaggatata ctggggaaga tgggtttgag atatactgta
    19381 aaaataaata tgtagaaata atttggaatg aagttctaaa ggttggagga gaagatatat
    19441 gtccagctgg tctaggttgt agggatactt tgagatttga agcagctcta cctctatatg
    19501 gacatgaaat aaatgaacac atatctccaa ttgaaggtgg tttatctatc tttgtgaaaa
    19561 caaataaaga atcatttatt ggcaaatcaa ttttatcaaa agaaaaagaa agtggagcta
    19621 aaagaaagtt agtaggattt gaaatgcaag gtaaaggaat gcctagaaat ggatatgata
    19681 taaggattgg tgataaaaca gttggatttg taacaacagg atgtgcatct ccaacgacag
    19741 gaaaaatttt aggtatggga attatagatt ctgaatatgc aaaagtagga aatgaaatag
    19801 gtatagcaat cagaaaaaaa gttgttcctg ctgtgatagt taagaaacct ttttataaaa
    19861 aacaatataa aaaagataat attatattaa acaaagaaaa taaattttca tatatacctg
    19921 ctacaagtga agataaatca aaaatgttaa aagtagttgg attaaattca gttgatgaat
    19981 tattttcaga tataccagaa gaagtaaaat taaaaagaga tttaaatttg gaaattggaa
    20041 aatctgaatt agaggtaagc aaaatagtaa aaagattgtc agaagaaaat ttaagtttag
    20101 aagacttgac ttgttttctt ggtgctggtg catatgacca ttatatacca tcaattataa
    20161 aacatataac ttcaagatct gaattttata cagcatatac tccatatcaa gctgaaataa
    20221 gtcagggaac tttacaagta gtttttgaat ttcaatctat gattgcagaa attacaggta
    20281 tggaaattgc aaatgcatca atgtatgatg gagcaactgc tgcaatagaa gcatgtataa
    20341 tggcaatgaa tcagactaga aaaagtaaaa tagtagtgtc aaaaactatt catcctgaaa
    20401 ctctgtcagt attaagaact tacttacaat ataaagattg tgagatagta gaaattgact
    20461 tttgtaatga atatgggact acagatatag aaaaattaaa agcttctgta gacaaagata
    20521 cagcatgtgt tcttatacaa actcctaatt tctttggaat tattgaagaa atggaagaaa
    20581 tagaaaagat aactcatgaa aataaggcta tgttaattat gagtgtagac ccaatatcat
    20641 taggagtttt aaaaacacca ggtgagatag gtgcagatat tgttgttgga gaagctcaat
    20701 cacttggaaa ccctcttaat tttggaggtc cttatgtagg atttttagct agcaaatcta
    20761 aatatactag aaaaatgcca ggaagaatag ttgggcaaag tttagatgta gaaggtaaaa
    20821 ttgcatatgt attaacttta caaactagag aacagcatgt aagaagagaa aaagcaactt
    20881 ctaatatatg ttcaaatcaa gctttaaatg ctcttgtagc ttcaatatat atggctacta
    20941 tgggtaaaga aggatttaaa gaggttggta tgcagtctat gaaaaaagca cattatacct
    21001 acaataaact tgtacaaact ggaaaatata agccaatatt taaaggtaag tttttcaaag
    21061 aatttgctgt acaaggaaat cttaatatag aaactataaa tgataaactc ttagaagaaa
    21121 atatattagg tggttataat ttagaataca attatccaga attaaaaaat tcaactttgc
    21181 tttgtgtgac tgagaaaaga agcaaagagg aaatagataa gttagttgga ataatggagg
    21241 gattataatg aaagaataca atagtttgtt gatagatata tctaaaaaag gtaggaaagc
    21301 atactcactt ccaaagttag atatagatga tataaaaata gaagatatga ttgacccaga
    21361 tatggcaaga caaagtgagt tgaatttgcc agaagttggt gaattagaat tagttagaca
    21421 ttatacatta ttatcaaata aaaactttgg agttgataca ggtttttatc ctttgggttc
    21481 ttgtactatg aaatataatc ctaaaataaa tgaagatatg gcagcacttc caaactttac
    21541 tggaatgcat ccataccaat cttcggatac agctcaaggt tctctttctt tgatgtatga
    21601 tttatcaaga agacttgcag aaattacagg tatggatgaa gttacactac aaccttcagc
    21661 aggttctcat ggtgagttta cagggcttat gattataaaa gcatatcatg aaaatagagg
    21721 tgaccataag agaaccaaag tgataattcc agattctgca catggaacaa atccagcaag
    21781 tgcagctatg gcaaactttg atgtgattca aatagcatct gataaaaatg gagctgtaga
    21841 cataaatgta ttaaaagagg ttttgaatga tgaagtagca gctcttatgc ttactaatcc
    21901 aagtactctt ggtttatttg aaaaaaatat aaaagaaata gctactcttg tacatgaagc
    21961 aggaggactt ttgtactatg atggtgcaaa catgaacgct attatgggga taacaagacc
    22021 tggagatatg gggtttgatg tggttcatct taatcttcat aagacttttt caactcctca
    22081 tggaggtgga ggtccaggag ctggtcctgt tggagttaaa aaagaattag tacctttttt
    22141 accaatacca gtaattgaaa gaaaagaaga taagtatgta ctaaattatg atagagaaaa
    22201 atctattggg aaaattaaaa atttctatgg taattttgga gttcttgtaa gagcttacac
    22261 atatatacta actatgggaa gggatggact taaagaggct agtgaaatgg cagttttaaa
    22321 tgctaattat ataaaggaaa gtataaaaga tgattatatt ttgccaatag ataccttgtg
    22381 taagcatgag tttgtattag gtggattgcc aagaggagag gccaatataa aaactatcga
    22441 tattgcaaaa agactactag attatggata ccatcctcca acaatgtact ttcctcttat
    22501 tataaatgag gcacttatga tagaacctac agaaagtgag agtatagaga cattagatag
    22561 ttttatagaa gctatgaaaa gtgttgctaa agaggcaaag gaagaaccag aactattgaa
    22621 aactgctcca cataatactt tggtcaaaag agtagatgat gcaagggcag taaagaaacc
    22681 tatactaaca tggtcacaaa gataaatagt taaaaagtag agatttaaag tataaagaga
    22741 attaaattta taagaaaaag ctatctaact taatctagtt tagatagctt ttttgtatat
    22801 tagtatatat ggtatttcct atataaagtt ttaaattata ttagtgctta tatagaatat
    22861 tgatatgatg attatgtata gatattaata agaattgttg aatttatctt gccttaactt
    22921 tatttactct tttatattaa gaaaaactta actaaaatat agcgaaattc ataaaaattt
    22981 aggaaatctt aagaattata atatttaaat ctttagattt atattttatt atataaacta
    23041 tagaataata aaataaaaaa attgagaatt ttataatata tttgtagatt tgagtataaa
    23101 aaagtgctaa gttgaaagga gtaaaatatg gataaggtta atttagaaag gtatagttgt
    23161 gatataaatg ttggtttaaa taaaaatcaa cttcaaagta gaatagatga taatttaatt
    23221 aatgcatttg ataatggaaa aaccaaaaca tataaacaaa tttttaaaga caatatattt
    23281 acacttttta atctcataaa tattgtttta gtctgtttat tagtttcagt tggttctttt
    23341 aaaaatacct tatttatatt tattgctgta attaatacta ttataggagt aatacaagag
    23401 ataagagcta aaagattatt ggacaatcta tctgttatag tttcaaataa ggtatctgtc
    23461 ataagagaaa aagagaaaaa agagattgat gtacaagaat tagtattaga tgatattatg
    23521 attttaaaaa caggtaatca aatttgtgca gattcaaaag ttttaaatgg aaaattagag
    23581 gtaaatgagt ccttggttac aggtgaatct gatattatta taaaaaataa aggcgatttt
    23641 ttatattctg gaagttttgt agtttctgga gaagcttttg taagagttga aaatatagga
    23701 aaagataact atgcaaataa gatagctaat gatgcaaaga tttctaaaaa acataattct
    23761 caacttagaa attcattaaa tgcaatatta aaaattgtag gtataataat aattcctcta
    23821 ggaattatat tgtttgcaaa acaacatttt ggaaatggag attctatagc tacatctgta
    23881 gtaggtacag tggcagctgt aaatggtatg ataccagagg gattaacatt acttacgagc
    23941 attgctctag ctgtaggaac tataaaactt gctagtcata aaactttagt gcaagaactg
    24001 tactgtgtag agactcttgc tagagttgat acactatgtc ttgataagac aggtacaatt
    24061 acagaaggta aaatgcaagt tgatgatata gtaatgttgg aagaagtgga tattaatgaa
    24121 ataatgggga atatatgtaa ttctctcaaa gatgataatg caacactaaa tgcaataaga
    24181 gataagtata atgttttaga tacgtataaa gctaagaatt taataccatt ttcttcagaa
    24241 agaaaataca gtggggttac ctttgaaaat aaaggtacat atttattagg tgcatttgag
    24301 tttatattta aggaaaaaaa taaaaaactt aggatagaag ttgaaagata ttctaaaaaa
    24361 ggaaacagag taatagtttt agctcatagt gattcttata tggaagatga taatattcct
    24421 gaagatataa agccactagc ttttatattg atattggata aaattagaga tgaagctaag
    24481 gaaactttag aattttttta caatgaaggt gtagatataa aaattatatc tggtgacaat
    24541 cctataacag taagtgaggt agctagaaaa gcaggtctta aaacagctag taattttatt
    24601 gatgcaacaa cacttaaaaa tgatagtgat atatacaaag cagttgataa atacagtgtt
    24661 tttggaaggg taagcccaca acagaaaaaa caaatgattt tagcattaaa aaaacaaaat
    24721 catacagtag ctatgactgg agatggtgta aatgatgtgc tagcacttaa agaagcggat
    24781 tgtagcattg ccatggcatc tggaagtgat gttactaaga atgtgtctaa tttagtctta
    24841 ttagattcaa actttgcatc aatgccaaag gttgttatgg agggaagaaa agtaattaat
    24901 aacattcaaa gagcatcttc tttgttttta gtaaagacaa ttttttcatt cttcttgtcg
    24961 ttattaacat tattcttctt cagtagacag tatccatttg ttcctataca attatcactc
    25021 attggggctg taacagtggg tataccatca ttcttcttag ctcttgaagc aaacaaaagt
    25081 agaatttcag gaaatttttt actcaatata ttaaaaaatg ctttaccagg agcactatgt
    25141 gtgacagtaa atgtaattat tgtttacaat atagttgaat atttgggtta tagttcaagt
    25201 gtattttcca caatgtgtgc tatattaact ggtgtatgtg gtcttttaat attaagacaa
    25261 caatgtttgc catttaataa agaaagatta tttttaatag taagtatgac tattgcattt
    25321 gttattggga ttctattttt aggtggatta ttctcatttg tagcattgag tagagaatta
    25381 attctcataa ctatggcatt agcaatatta atgccaatta tgataaaagt tatgttattt
    25441 gtcttagatg agatattaga gacaattgtt ccagatttaa attaatttta aaatgatata
    25501 aataagagca gttttagttg aactgctctt tttagatgtg tttaatttta tgtaagaata
    25561 tgtattttta ttaatcagct atatatgagc aacagtagtc aacaacttca ttaaccttta
    25621 agtcaaaact tgaatcacta ggaacattaa atttttgtcc ttctttgtaa gttacaaact
    25681 cattgcttcc aggcagtttt acatccatac ttccagcaag catctccatt atttcttctt
    25741 ctctagtaga aaaagtgtat tctcctggca acattaatcc taaagttttt ctttctccat
    25801 taggcagtat tattactctg ctacttacct taccatcaaa ataaacgttt gccttcttta
    25861 ctgcagtaac attcttaaat tcactcatta gataaaacct ccaaaattat ttgtacatat
    25921 atattatata aaaataatgt aaaaaatgct atatatttac aaaaaaattt agtatttatt
    25981 aaaaaaatta ataagaataa aaaagagatg gaattgctta aattgataaa aaatataaaa
    26041 taaattgcaa agtaagcaag aaaagaataa gttgaagagt tatatattat aaatattaaa
    26101 aattttagta ttaataaaaa agatattaca ttaatatgaa aatataggag attagaagtg
    26161 tttgataatt acagtacatg ttagatatat cattgtaaat taacatagct ttgaatataa
    26221 taatgttcaa aaggcaatat ttttgaggtc tgaatccaat aataatatag tcttatgatt
    26281 gtagatatca aaaaatataa gttactaaga atacgttttg tagatttata tatgattaat
    26341 aactaaagct ataaataaac aaaataaaaa gagtatgttt caacctatag cttttagatt
    26401 taaaatatac tcttttatta attatagttg tataaattaa ccataatata taattaaaat
    26461 ttttattata attatatatt aggttcttca attagttttc cacatacata tctatgaatg
    26521 atatttctat catcaccaac atagataaat ctttcaagtc tttcaagtat agaataattg
    26581 tcatgattta aatttgaatc atcaataatc aatgcatcaa aatcataacc ttcttcaaag
    26641 ctaccaactt ttccaaagaa acttccacca cttttggttg caatataaaa agtctcagat
    26701 aaagataaga aattatactt cttaccagag ttaacccaat ataatttaga taattgtatt
    26761 gcagatacca ttgctttaaa tattgacaat gtatgtccac cacttatatc tgatccaagt
    26821 gctaatctaa tattattatc gattaatttt ctgataggca tagcaccact accaacatta
    26881 aagttagaag ctggacagtg aactgccata acattatttt ctctcattaa atctatttca
    26941 tcctgagaac aatgaacaca atgagccatt aaagtaggag tttgaccaaa taaattatat
    27001 ctattatatg catctccata gaatttagat tcaggttcta gacttctaac ccattcaatc
    27061 tcatcataat tttcagataa gtgtgattga ataggaatat tatattttaa acataaatct
    27121 cctaaacctt ttaaaagttc acttgtacag cttggtataa atctaggtgt tattattggt
    27181 tttacaatag aatctgtatc actatactta tttataattt cttctgtatc agtaatagaa
    27241 tcctgagtgt tttctaataa agtatcaggg caattcatat tcatatttac tttaccaacg
    27301 tatgctccaa gaccaacttc tttgaatata tccataagtt tcatagaggc ttctttatgt
    27361 actgttgcaa acactgctat tcttgtagtt ccagatttcc aaagttcttt tacaaatttt
    27421 ttatacattt ttgttgcata ttctatatct ttataatttg attcttctgg aaatgtataa
    27481 gtttcaagcc atggcaaaag ctctttatcc atagccattc caagattttt aaattgagag
    27541 gcatgggcat ggaggtcatt cataccaggt attataagct tatcagtgta atctactact
    27601 tttaggtttt tgtattcctt aggtatctct ttaaatattc cctttacttt acctttttcc
    27661 agtataatat aactattttc aaatactgtg aaagtttctg aagtttttgt aaaaattata
    27721 tttcctttta atacttttaa catataggtc tccttagttt taaatttgtt ttaatatatg
    27781 taggtaaaat tttgttttac aaatcaaaat tttaccacat gtattatatc aaatctaaac
    27841 tataaatact attagataat agcaaatatt agtgaaatac agttgtttac tattattata
    27901 tataactgac atttaagtga catttaagaa aaatataatg cctacttaca taaaatggaa
    27961 tgttatttaa agagaacttt gattatattt tcagaagctt ttttatccat atcgtttaaa
    28021 atatgagaat atctatttat agttattttt atattagtat gtcctaatct ttcagagatg
    28081 atttttatat tagttctagc tagaagaaga actattagaa tagataatat agtaagtatt
    28141 tacaaatatg taggtgttct taaattgata aattattcca ttttaatttt atagtttgaa
    28201 ttttatgata taataaaaat atataaattt tgcagtgagc gatatttttg ataaagtagg
    28261 gtttaacagt tgcaatgtaa gggattgagg gtgtatgata aatgttatca attgcactac
    28321 tcatggttca ctgcaaattt gagagagttg tatgtgtgta agtactgaaa atacttagtt
    28381 tattttgggg ttttatatta actaagtggt atgtaaatag aatttagtac tttctaaaaa
    28441 tggattgcct agcaagtttt atattaacta agtggtatgt aaattttcct aattccctat
    28501 ggtgtagcat tggttgatag agttttatat taactaagtg gtatgtaaat atattaaaga
    28561 tatgacactc ttttatataa aatataagtt ttatattaac taagtggtat gtaaatacaa
    28621 gttcagtagg gagacagaca gtaattttaa aagagtttta tattaactaa gtggtatgta
    28681 aatcacaaca atacaaataa aactctgaaa aggtaaaagt tttatattaa ctaagtggta
    28741 tgtaaattta ttattattat atatcttgac ttagcactat ttttgtttta tattaactaa
    28801 gtggtatgta aatattactt tgaaagcatt aacagaaaaa gagattagta agttttatat
    28861 taactaagtg gtatgtaaat tatttactgt tgaacctaca tcttgattgg ataaaaagtt
    28921 ttatattaac taagtggtat gtaaattaag gttttaattt attagtaata aatataagac
    28981 ctagttttat attaactaag tggtatgtaa ataattcttt gactgcatca tttaaataat
    29041 ctagctgagt tttatattaa ctaagtggta tgtaaattct aaaaggtcac atatatttat
    29101 taaatctgtt gccggtttta tattaactaa gtggtatgta aatttcatag tctttagagt
    29161 ctgtaaatac tttactcata gttttatatt aactaagtgg tatgtaaatt tactttaaaa
    29221 tatgtaccat aaaaaaatat atcaagtttt atattaacta agtggtatgt aaatatctct
    29281 ggcgtataac cttcttttcc tattagttct ggttttatat taactaagtg gtatgtaaat
    29341 agggtttctg ataaaatctt caaacatgta aaatatgtgt tttatattaa ctatgaggta
    29401 tgtaaatctt atacttagtt agaactatat atcgacacaa atatgcttta tattaactat
    29461 atggtatata aattgcaatt tttattcgtt gtccaatcgc tttgaaattt gttttatatt
    29521 aactaagtgg tatgtaaatt tgcaacaagt ataggtaaaa taccccaata atttatacag
    29581 cattttctcc tttaaaatat aattattttt tatcatttgt agtaaataat taccacataa
    29641 tattgacttt agttttaatg attaaaatat aaaagtagaa taattataaa aagtattgaa
    29701 aaatttataa atatatataa taaaacttaa tgacaagata ttagatataa aaaataatta
    29761 ccttataaat agattgaaat ttatgaatat tcatactata atttaaatat aaggagatgc
    29821 cctttgaaaa taaaaattaa aaaatattta atgctattac aataggaact agaactacac
    29881 ttaataaata tacagaaatt agatttggct caatataaaa tacaaatata gaatttaggt
    29941 gttttttatg aaaaaatttt tatatgcttt atatagtttc attgttatta tagctaattt
    30001 tagattaaaa gaaaaaaatt ataactttat tttattagca atctttttta tactgctgtt
    30061 aattca
//
LOCUS       DAELNY010000029        30057 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.029, whole genome shotgun sequence.
ACCESSION   DAELNY010000029 DAELNY010000000
VERSION     DAELNY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.029"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(32..802)
                     /locus_tag="KPE55_001130"
     CDS             complement(32..802)
                     /locus_tag="KPE55_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG0492225.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(804..2231)
                     /locus_tag="KPE55_001131"
     CDS             complement(804..2231)
                     /locus_tag="KPE55_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0492226.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            2622..3344
                     /locus_tag="KPE55_001132"
     CDS             2622..3344
                     /locus_tag="KPE55_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0492227.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            3541..5208
                     /locus_tag="KPE55_001133"
     CDS             3541..5208
                     /locus_tag="KPE55_001133"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG0492228.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(5304..6623)
                     /locus_tag="KPE55_001134"
     CDS             complement(5304..6623)
                     /locus_tag="KPE55_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0492229.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQVAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGSPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(6664..7410)
                     /locus_tag="KPE55_001135"
     CDS             complement(6664..7410)
                     /locus_tag="KPE55_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0492230.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(7654..9663)
                     /locus_tag="KPE55_001136"
     CDS             complement(7654..9663)
                     /locus_tag="KPE55_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0492231.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(10057..11490)
                     /locus_tag="KPE55_001137"
     CDS             complement(10057..11490)
                     /locus_tag="KPE55_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0492232.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(11577..13493)
                     /locus_tag="KPE55_001138"
     CDS             complement(11577..13493)
                     /locus_tag="KPE55_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0492233.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLVAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(13567..14427)
                     /locus_tag="KPE55_001139"
     CDS             complement(13567..14427)
                     /locus_tag="KPE55_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0492234.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(14778..15965)
                     /locus_tag="KPE55_001140"
     CDS             complement(14778..15965)
                     /locus_tag="KPE55_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0492235.1"
                     /translation="MIESIKKRAYEIENGLGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(15979..17370)
                     /locus_tag="KPE55_001141"
     CDS             complement(15979..17370)
                     /locus_tag="KPE55_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG0492236.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(17965..19665)
                     /locus_tag="KPE55_001142"
     CDS             complement(17965..19665)
                     /locus_tag="KPE55_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0492237.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(19637..21040)
                     /locus_tag="KPE55_001143"
     CDS             complement(19637..21040)
                     /locus_tag="KPE55_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0492238.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCNKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(21304..21795)
                     /locus_tag="KPE55_001144"
     CDS             complement(21304..21795)
                     /locus_tag="KPE55_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0492239.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(21821..22633)
                     /locus_tag="KPE55_001145"
     CDS             complement(21821..22633)
                     /locus_tag="KPE55_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0492240.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKETLKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(22646..23797)
                     /locus_tag="KPE55_001146"
     CDS             complement(22646..23797)
                     /locus_tag="KPE55_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0492241.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            24067..24708
                     /locus_tag="KPE55_001147"
     CDS             24067..24708
                     /locus_tag="KPE55_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG0492242.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            24734..26278
                     /locus_tag="KPE55_001148"
     CDS             24734..26278
                     /locus_tag="KPE55_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0492243.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(26478..27104)
                     /locus_tag="KPE55_001149"
     CDS             complement(26478..27104)
                     /locus_tag="KPE55_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0492244.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(27239..27955)
                     /locus_tag="KPE55_001150"
     CDS             complement(27239..27955)
                     /locus_tag="KPE55_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG0492245.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRSDNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            28381..29595
                     /locus_tag="KPE55_001151"
     CDS             28381..29595
                     /locus_tag="KPE55_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0492246.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
BASE COUNT         9999 a         5245 c         3412 g        11401 t
ORIGIN      
        1 tttttaatta aaactttagg taatctattc tttatatata attattagac ttgaaaatat
       61 ctttaaaatc aaccaattca atattatttc tttttaacca cattttaact tccttatcac
      121 atagcattgc atgttcatat actctctctt gaataagaga agatgatttt ataagactat
      181 aatctataaa tcctggatga gtaaccatca atgttatatc tttatctaaa tttatatatt
      241 tttcttcaaa taatttaaca aaagatttat ttaacttgta aaaatcattc tgaggatatt
      301 tacttggcat gctaatacat gaatccaaat aatagggaca acttggtata ccgtattctt
      361 ttgcagcatc acacattgct gatattgata catcttcttc tataccatgt atatcaatat
      421 actcaggcaa tttactcata tacaacttgt aagtttcaat ttgagcacaa acttcttcat
      481 atacttcttc ataattaaaa acctctttac ctgtcagtag ttgcgttctt cttattttgc
      541 tagaaacaaa ttttccacct tcatctaaca aattctttat cttaatatgt tctttgcaac
      601 aaggttctcc tattactaaa ttaacatgta aaccaaaaca tacatcatca ttttttacca
      661 tttctatagc ttgtcttgaa aaaggcatat tcaccatgat tccagcactt gtaacaatac
      721 cgttcttata agaagatatt atccctagtg taacaccttc tgaatatcct atatcatctg
      781 ccctaactac tagtttcttc attttatgcg tctgcctgct ttctcaactc atcaagttgc
      841 atttccatct catcctttac tttaacaaca tccgttccta tgatgatttg aactgatgta
      901 ttactcaatt taattacccc tttggctcct gtttttttaa tattatcatc attcaaaata
      961 gtagcatcct tcacttctaa acgtaatctc gtcatacaat tattaagagt cactatattt
     1021 tcataaccac cacaattttt aattatctgt ttagccatat atgaaaaatt accatgagaa
     1081 agttttaaat tcttttcttt ttcactaact ttatcaccta tctctatctc aacttcacgt
     1141 cctggtgttt gcacattatc ttttattatt atatatttaa atgttacata atataataca
     1201 aagaatacaa ctcctacagg tataactaac cacgctttat ctccataact aaaacttaag
     1261 acatagtcaa ttatacttgc tccattaaat gaacccaagc gaacacccag taaatatagt
     1321 acaactccac ctagaccaga ataaaaagaa tgaattatat ataactttgg agatgcaaat
     1381 aaaaacgaaa attctattgg ttcagttact gttgccataa aagaagtcaa tgctccagcg
     1441 gtcattgcac cttttacttc ttgcttcttt ttatcttgtg ctgttttata tatagcataa
     1501 gaaatagcag gtattgaaaa catcataatt atgtaaaatc ctgtccagaa gaatcctgca
     1561 tttgggtctc ctgccataaa tctaggaatt tctcctctta taacttctcc tgttgatgtt
     1621 gtatattcac ccatttcaaa taatatgtaa gcatttaaaa cttgatgtaa cccagttggt
     1681 attagtgcac ggttacagaa taaaaacaag aatattccta ttgcaccagc tcctcccaac
     1741 caaaaaccaa attgattcat tgcagcttgt ggatatatcc ataataaact aaatatatat
     1801 gataaaatta aagcaaccac tggtgatagt gtaattacac atttttctcc tgcaaagaag
     1861 cttaaaaatg ctggaacttt aactttatat gttttattgt aagtccatgc tatacaagct
     1921 ccaacaatta tcccaccaaa tacacctgca tttaatgtat cattcaataa ctctaaattt
     1981 gacttaaata tcatatatgc aactacggct gcaaccatta cacttgtctt gtctttagac
     2041 tttgagtagg cactagctgc tcctaataaa aataagtagt ccacatttga aaatactata
     2101 ttagatatat tagatatagc tggcacattg agcatatttg gaccaaataa taatcctaat
     2161 atacctgctg ctggcattac agctattggc actagcatag tctttcctaa aagtgacagt
     2221 tgttgcatca ttttgtttaa gaacctcttc atatccaatc ccctttctat ttcggtttaa
     2281 ttttttgtga aaacgatttg ttcacatttt atattttaaa tattatattt gtttaaacaa
     2341 attgtcaata ttttttattc attttatttt tatcatttaa tttaacatat aaatcctttt
     2401 aagtgtattg ctatatctag tctatttttt taattcattt tttttatttt aaaaatatgt
     2461 ttaaccatgt atttgaaaca acccacaaaa taattctatt ttgtcctttt tatggttgat
     2521 atgattctat tttcactata catgtctaaa cacatttgat ataatttgtt catatcagtt
     2581 caataatatt actaaagacc tttaaggagg gataggggta tatggctaaa aataaatttt
     2641 cagagatata tgaagtattg aaagaggaaa tacttgatgg taaatataca agtaatatga
     2701 tgctgcctac tgaacttcaa cttattgaaa ggttttcatg tagcagaaac actgtacgta
     2761 gagcaatttc tcaattaaac acagaaggat atgttcaaag tataaaaggc aagggtgttg
     2821 tagtgttaga aaatagttgt tctaatgatt tttttctaaa tatgcacaat ttcaaaggtg
     2881 tagaatcgat tgtagaagat aaaaaggtta atactgctac tagtgttctc cacttcagca
     2941 aaatcttaat tgataataag ttatctaaaa agacaggttt taaagttggt agtgaagtat
     3001 attaccttca cagactaaga tatattgata acatacctaa aattctcgac attaactatt
     3061 ttctatgtag tatagttaaa gaccttgatg tttctatagc acaaggctct atttataaat
     3121 atatagaaga gtgtctaggt acaaaaattg tttcatccag aaagatattt aaaattgaaa
     3181 aagctacaga gcttgagtta aaaacgctac ctctaaatga ttataattgt gttggtgtta
     3241 ttaaaaatag tgtatatact gatgatggaa agctttttga atatactgaa tcaaagcata
     3301 cacctgaaac atttgtattc atggatgtta ctcaaagata ttaaaaaaat tatgtttaat
     3361 taaacataat ttttttacgt ttagacactt tatcccatac taacctcact attctatata
     3421 caattatatg tttatacaaa taattattta ttgtatattt tagctttaat attagtttaa
     3481 cgcttgacaa tttgtttaaa catattataa ttaacttata aattacagga ggtatcaagt
     3541 atgaaaaatt ggtggaaaaa agcaactgtc tatcaaatat atccaaaaag tttcaaagat
     3601 agcaacaatg atggtatagg tgatataaat ggtattatag aaaaattaga ttatctgtat
     3661 tctttaggtg ttgatttatt atggttgact cctatgtacg tttctcctca aagagataat
     3721 ggatatgata tagaagatta ttataatata gacccaaaat atgggacaat gagtgacttt
     3781 gaaaaactct taaaagaagc tcataaaaga gacataaaaa taatgatgga tatggtccta
     3841 aaccatacat ctactgaaca taaatggttt aaagaatcta aaaaaagcaa agacaatcca
     3901 tatcgtgatt attatttctg gaaagatgct aaacctgatg gttctgttcc taataactgg
     3961 atatccagat ttagtggaac tgcttggaaa tatgatgaaa ccactaacca atattattta
     4021 catttatttg aagaaactca agctgattta aattgggaaa atgaaaaggt tcgtgaagaa
     4081 tgttacaaaa tactagaatt ttgggctgat aaaggtattg atggttttcg tttagatgta
     4141 gtcaacctac tctccaaaac acctggatta cctgatgacc ctataacagg accaaaagga
     4201 gatggaagaa ctcactacgc tgatggtcct cgtatacacg aatatttaca taatatgaat
     4261 caaaaagttt tcaaacctaa aaatatagtt actgtaggtg aaatgtcatc tactacacct
     4321 gaagagtgta tcaactatac tagagaaaat cgagaagaac tatctatggt ttttagcttc
     4381 catcatatga aaacagatta taaaggaggc gcaaaatgga ctaatgaaat gttctctctg
     4441 gataaattaa aaaaagcaca atctgacttt caatataaaa tgtatgaagg aaaaggatgg
     4501 aatgctctat tttactctaa tcacgaccaa cctagagctt tatctagatt tggagatgat
     4561 agattttttc gtcaagaaag tgctaagatg ttagccataa cattatttgg tctacagggt
     4621 actacataca tatatcaagg tgaagaaata ggtatgacaa atgcctactt tactaaaata
     4681 gatgaatatg atgatgctga aagtaagaat gcatactatc atatgattaa atctggtatt
     4741 gatgaaaagg aggcattgct tatacttcaa caaaaatcca gagataatgc ccgtactcct
     4801 atgcaatggg ataatactat ttataagggg ttttctaatc ataaaccgtg gttaaaagtt
     4861 aataatgaca atattagtgt agaaaatgag cttaatgatt ctcgttcagt attttatact
     4921 tatcaaagat taatcaaata tcgtaaacaa tatgatattt ttacagaagg tacttataga
     4981 ttgttagatg aaaatcataa aaatctattt gtttatgaaa gagagtataa aaaccaaaat
     5041 cttcttgtta taagtaattt tactcatgat aatgtagtat ataaattacc tgaaacttat
     5101 ttgaataaat taaactacac aatacttgaa accaattaca ctagagatat aattgaaaat
     5161 aaaatggaaa taaaacctta cgaaagtata atgatatatt ataaataaag ttatttatgt
     5221 aaaaatcggt ataacaccct ggtgttatac caattttact aaattcaaag taatatttat
     5281 agaatatcta tagattacaa taattatttg acttctacat cattatattt ctttgaatat
     5341 actttagtca tatagaaata tataggaact acaattatca tcccacctaa tccccaaact
     5401 atcttcatag gttcatcaat acctgctttt atcagtaata atatacttat aataactgca
     5461 aatattggta taattgctcc tccaggtatt ctaaaattta catctttacc agcatacttc
     5521 tttctcaata ttagtacagc taaacatgta ggaatatatt gaataaatct agatacaaca
     5581 cttatttgag ataatgttgt gaatgaaccc gaccatgcta ttagtaaagt tcctatgaca
     5641 gatactataa ttgctatata aggagatcca tttttattag tttttcttat agacttaggc
     5701 attaatcctt gttcaaccaa agcagcagca gaccttggtg taaatatgga tgatgcaata
     5761 tttatacctc caacagatac tatagttcct attgtaatta tatacccacc tataggacct
     5821 aacatcttaa tggcagcatc tgcaacagga gtagctgacc ctgctaagcc attacccaac
     5881 acccctatac ttattcccag tataagcata tatattactg atactacaaa cattaccatt
     5941 accaatgctt taggtacatt tttcttaggg ttctccatat cttttgctgc aacagctaaa
     6001 gattcaaatc ctgtaaaagc ataaaataaa gttatagata ctgctacaaa agctgatgac
     6061 gcactttgac ctttttgtat tattacaaat ggggtaaaat tagaaccctt tataaagaat
     6121 aatcctacag ctataaataa aactattggt aataattttc ctatagtcat gatattattt
     6181 aagtgcttag attgtttcat tcccataaaa tttataatcc caagccctac aactaatata
     6241 ccaattatta tatttttaat aagtggttct gctacttgtg gccatacaga tgatagtgtt
     6301 acagcaaaga aatttgccat agtagcccaa gcaatcatac taactatcca cttcataaat
     6361 ccaacttcaa atccaacaaa atctccaaat gcctcttttg cataaagata gggccctcca
     6421 tcagtatcaa accaactacc tgcttcagca aaacataaag caatagaaac agctaaaagc
     6481 atacaaaaaa ctaatactaa tatacttgct ggacctatat ctgtataagc atcaccaggt
     6541 aacccgaata tacctgaccc tattattgca ttgattccta atagtacaat actccaaaaa
     6601 cctaacttaa tgcctttttc cattgttatt ccccccaaag ccaacttaat atatttatat
     6661 tttttattta gatgcttctt taactattgc cataaatatt tttttcatat tatcataatc
     6721 tctagtcatc atttctggat gccattgtat ccctattaca aactcacttc cttccttttc
     6781 tatagcttca attaaaccgt ctttagatgt agctgaaaca atatatcccg gtgctacttt
     6841 gttgacaact aagtgatgaa aactattaac taaaacttct ttttctccta gtatttcata
     6901 taattttgtc ccctccttta tctgtactgt atgtgtaggt acattagata gatgttgctg
     6961 attgtgttta atataagcac cttcaattaa agataaatct tgatacaaac tacctccttc
     7021 agttacattt ataatttgtt ctcctctaca tatacctaaa actggttttt tcatatctaa
     7081 agcatgtttt aataatttca tatcaaaact atctctcttt ggtgatattg cccccaactt
     7141 attatgaggt tcttctttcc atatgagtgg atttacatct tgtcccccag atagtataag
     7201 cgcatctata ttagatacct gctcctttat aatctcatca tcatatacaa tcggtacaat
     7261 atatggtata gctttacaca taactactga ttgtatataa tcattattta cataagccct
     7321 ttcatagcca ggaaacattc ctccttcatc aattagtaaa ttacctgata ttcctattat
     7381 tggatatttc tttttagcca atttcttcac cccttaactt atttgtgaac aataaaaaat
     7441 gctacttctc ttaaagagca agtagcatac caagatttta tttatttgaa cattataatt
     7501 tattttagaa atactttttt tgaatacgag taatataaat aactttattt acttatattt
     7561 tattttaatc ctgtttagtt tgactttttt aaattgtttt aagtacaact aataggtaaa
     7621 ataggcattt agattttcct ttttatcaac ctattaaata ctgtttaata tattttttcc
     7681 ccttctattt aattcacttc cctttctacc agattcacat attatcaaat ccagctcttt
     7741 taacatatta agtattcctc ttacctcacc ttctgttata ttgtagccta attcatttat
     7801 tgaattaaca ataccatttc tacctatatt tttattaaga ttattggcat tttttaaaca
     7861 ctccattact aactttactt tctctataga tgttctatcc tttaatatgc ttatttcttt
     7921 actaaaatct tcagttatat ttaataagtt atgaggtaaa tcatttatta aaataacatc
     7981 ctcagaactc ataatgttaa tatacatagc agtatttttt aattctctta tattacctgg
     8041 ccattcatat gcattaataa tgtcttgaac atcttgacta attgctctct tccttttcat
     8101 aaaatgcttt aacataactt ctatatcttc ttttcgctct cttaaaggtg gtatatttat
     8161 gggcaataca tttatcctat agtataaatc tgctctaaat ctatctcctt ttatcatttc
     8221 taataaattt ttatttgtag ccgcaattac tctaacatct atatctatta cattttctcc
     8281 tccaaccctc attacttgat tctcttgtaa tactctaagt aatttgcttt gtaagtatat
     8341 aggcatgtct cctatttcat ccaaaaatat agttccattg ttagcctgtt caaataatcc
     8401 ttttttacct ccttttaaag ctcctgtaaa agcaccttct tcatatccaa aaagttcact
     8461 ttcaagtaaa ttttctggca tagctgcaca gtttattgct ataaatggct gactatgtct
     8521 tggtgatgca ttatgaatag actgagccat gagttctttt cctgttccac tttctcccgt
     8581 tataagaatt gtataatcag attcagctat tttcatacct aatttcttag ttctttccat
     8641 cttcgttgaa actgttttta tatcatcaaa agtatatcta gcaacttgtc ccttttctct
     8701 aagcttcttt gataggtttt gctccaattt tcttatgtaa gttatttctt gaatacaaaa
     8761 ataatatcca gtaacccttc caaaactctc tatattatac ttatttacgt ttaagtactt
     8821 attattaaat tttataactt catcatgtgc cttactcaaa ctaaaaatct ttttaatatt
     8881 ctcattctct aattcatcta tatttttccc tataatatta ctttgaatat ctagtatatc
     8941 ccttaaacta ttattacaaa taattatgtt tcccttatct gataccattg ccatacctaa
     9001 gtttgataaa tttattatgg aatttagttc atctgctttt atagtaagtt ctttgtattt
     9061 agtatttatt cctgaatata aactaattat ttcatctgta tacttaatta gcctctcagt
     9121 aatttcttta tcatccagtt tgagttttgc tattatttga ataaacgttg aactatcaat
     9181 atacctatgg cctaaatcaa taacttcttc tatatatttt ggaactaaac ttgactctcc
     9241 tggcgttata gctatcttaa tatttctata ttcttcattg ggattgtatg gtataaaatt
     9301 caaatgattt attcctatat tgtataaatt agatattgtt tccaatacag tatgcttatt
     9361 atcgtttact actaatacat ctattccttc tggcaatgaa aataacttat atatatcctt
     9421 ttgctttatg ctcctattta tagttattat ctttgagaca tcttccacat atttttttat
     9481 ttttaatact ctatcatcta tcattaccaa tattatgtca tcttttatta attccttctc
     9541 tgttatttta tttaaataat aattttttat cattacttta tctttaaaga tcatttctag
     9601 atttttattt aagaaacatt ctaatttaga caacccatca gttactactg ctattgtttt
     9661 catttgatta ctccttttaa aatatactct actcttttat aatatacatt tgattttcac
     9721 tttaataaca caaaaaattg tacatttagt atactaacat attcctttat tagttttatg
     9781 tccataaata tcatagttgc aatatctatc atatttctta aacttctata aagcaaaaat
     9841 tgtgccaaat taataatttt cttaatttta ttctaaagtt cctcaatagt atttacagac
     9901 ttagcactta tagcttataa gtctttatat tattaatcta tttttatagg ttaattgtac
     9961 aaatcatata tcacttttac ctattaaatg cattataaaa aattgtattt ataaacacta
    10021 tttactactc ttctaagtac aaacagtatc tacatactat aacctttcac cattagattc
    10081 tattacttgt ttataccagt caaaactctt tttcttaatc ctagacaagt ctccacttcc
    10141 atcatcatac ttatccacgt atataaaccc atatctcttg gccatctcac cagtagatgc
    10201 acttactaaa tctatacatc cccatggagt atatccaaat aaatcaacgc catcttctac
    10261 agcttccttc atttgctcta tatgtgcttt taaatactct atacgatagt catcatttat
    10321 cactccatta gattcttttt tatcataagc tcctaaacca ttttcaacta ccatcatagg
    10381 tattctatat ctatcataaa tttcatttag tgcatatcta agcccttcag ggtctatttg
    10441 ccatccccat tcactagact taagatatgg atttttcact ccacctaata tgttgccact
    10501 tgtattatct tctttagaat cactacttac acatgtagac atataataac taaatgtata
    10561 aaaatctaca caaccttctt ttaaaatttc taaatctcct ttttccattt ttatttcaat
    10621 attattttct ccaaaatatc tattcatata agttggatat tcgccacgca cttgtacatc
    10681 tccacagaaa taattcatta ctctactatc tctttgagtt ttaactgcat catctggatt
    10741 acaagtataa ggatacattg ttgcaaatat ttgcatacat ccaacttgat aatttgaatc
    10801 tatttcatga gctaacttaa cagccttagc acttgctaca aattgatgat gtaacccttg
    10861 aaaacgaagt tgaggtatat ctacttggtt tataaaatct gtagtccctt catttaatat
    10921 tccttgtgat aagtagcctc ccataggcat tgtagctgaa tttatttcat taaatgtcag
    10981 ccaatatttt acttttcctt tatatctttt aaatattgtt tcacagtaat tcatatacaa
    11041 gtctattaca tctcttgaaa cccatccatt gtatttctct gttattgcaa atggtatttc
    11101 atagtgagat attgttatta atggttctat attatatttt aaacattcat ctatcacatt
    11161 ttcataaaat ttaagtcctg cttcatttgg ttctttctcc attccagttg gaaatattct
    11221 agtccatgct attgaaaatc taaatacctt aaatcccatt tctgcaaata aagctatatc
    11281 ttctttgtat ctgtgataaa aatctattgc ttcatgactt ggataaaatg ttcctttttc
    11341 tataactctc gttattcttt tacttacttt ttgagaacct ccagtacaca tatctgaaca
    11401 agaaggacct ttcccatcta aattccatgc tccttcacat tgatttgctg ctgttgctcc
    11461 accccataaa aaatcttttc taagtgccat aattctacct ccaataattt atttatagta
    11521 actttatttt aaaataaaaa tacatatata taattcttaa ttaatagaac tctcttttac
    11581 gctaaaacag ttatcaattt ctgactatta tttacatgct tttgtgtatt ttctacaata
    11641 tccacataat cagaataatt agtaacaact actggtgttt ctaagcaata tccttctttt
    11701 gaaatacttt ctatgtcaaa agttattaat gtctgacctt ttttaatctt atccccttga
    11761 ttaacaaaag atttgaaata tttaccttcc aactgaatag tatttaaccc tatatgtatc
    11821 aatagttcac aaccattatc agatataatt cctattgcat gttttgttgg aaataaagtc
    11881 attacagtcc catcaaatgg tgccactact tttccttctt taggtgttat tgctacacct
    11941 tttcccaata tacctgttga aaacgctgca tcttgtatct gctccaaagc ctttacttca
    12001 ccttttattg ggctatatac tacttctctt ttgttttttt ctataacgcc ttgcccatta
    12061 tcatctttag aaatttctac tatagcatca tctttccaaa aaaataatgt taaaacaaat
    12121 cctactacaa tagctactat tattgctatt atagttgcat aaacaccttt catatctcca
    12181 gttgttggat ttatataatt aggtatacca aatattccaa gtccacccat tacataactt
    12241 tttacattca ttatccccat tacagctcct gccacagcac cacctacaca actatatatg
    12301 aatggtgttt ttcttggtaa agttattcca tatattgcag gctctgttac accacatatc
    12361 cctgaaataa ctgcaggtat acacagctct tttatcttct tatcttttaa tttaaagtac
    12421 attgctgcta ctactgctgt ttgtgcaaaa gatggtacaa atgctcccac taatattgtg
    12481 tcaaacccca tagtcataag gtttgacata gctattggta ctaatcccca atgtagacca
    12541 aatattacaa gcacttgcca caataaacca gttaatgcac cacataaaat tggtgaaaag
    12601 ttaaataaag ctaaaaatcc atttccaact atattagaag cataggtagc tataggccct
    12661 ataaccataa atcccaatgg taaagaaact aataaaactg ccattggtac aaagaaattc
    12721 tttactacat ctggtattat tttagcaaac actttctcca acttagctac taataatact
    12781 attaaaatta ctggtactac tgttgaagtg tagttcattg ctattactgg taatcctaag
    12841 aaagttatat atactggtga ttcaaatatt gttccagtaa atagattgta taaaggttga
    12901 cctgtagatg ctagagtatc taattgaata cctgaatagc ataaaactgc acctataagc
    12961 attccaataa atggttttaa tttgaatttc ttagcagctg tatatccaag aattattggc
    13021 atatactgga acattgcatc tccaattgca tttaacataa tatatgcacc atcagtttct
    13081 ccatacaaac ccaatgctgt aaatactgca ttaaatcctt ttatcatacc agcagcacac
    13141 agaactccta atattggttg aaaaactcct gatattatgt ctataaactt attaagtaat
    13201 ccttttggcg catctgagtc tgaattatct attgtatctc ctcctaatcc agccaaatcg
    13261 cacacaactt cataaacatc tggaacatga ttaccaataa ctacttggta ttgacctgca
    13321 cttttcatta cagtaactat accatccatt ttttttagca catcatcatt tgctattgac
    13381 tcatctttta atttaaaacg tagcctcgtt atacagtgag ataaactgct aatattatcc
    13441 tttcctccta cattcgcaat gatttctttt gatagttgtt catactttct cattttttct
    13501 cctccatttt ctttttgaaa tatgaaggtt tggccaactt aatgtcacca cccaatatat
    13561 ataaacctaa ttattaggat ttatatctga cttaatactt ttacttacca ctctttcaac
    13621 gtgaatagtt aagtataatt tttcctcatc tgttaaatcg tagttgtatt ttttatgtat
    13681 aaattcatcc aacttttcta tgaatttata agcattttta tatttaactt ttattacatt
    13741 taataaatca tcttcattat tttcatgtaa attattacta acaattcgtt ctgcaaaata
    13801 ctttaagtga gtaataaatc tatgataata aactgaatct tcatcaaata ctattctgta
    13861 atggtattta accatatttg aaatttcttg tattatttgt gtaatctcat atatagtttt
    13921 tatactttga tccatttgtg cattaactat atgcagtgct ataaatccag cttcatcttc
    13981 tgatagtcta atttcaaatt ttttttctat ataatccaat gcctctaacc ctattttaaa
    14041 ttcactctta taaaatcttt ttatatccca aagtaataca ttttttacac ttactccttc
    14101 acgagctctt tctattgcag tatacatatg gtctgttaaa gaaatataaa tactatcatt
    14161 taatcttttc cctagttttt ctttagcata taaaattatt tcatctgata attccatata
    14221 tcttatagga atatctgcta ttagttcttt aaatttatca gatatatttg ggtctgaaag
    14281 tttgaatatc ttatcaattt tatcctttga tatatgctct cctgtacgtt ttttataagc
    14341 taaacctctc cccataacta ctatttcctt atgattttca tcagttgtaa taattacatt
    14401 attattgagt attttttcaa taatcattgt gatgccccct ttattagcat taagttaaaa
    14461 taaatctttt ttaattattt agaatattta aaatgtaatt tattacataa aaaaaaacct
    14521 atttattaca cattaaatag actcccatga gtcctgagta ataaataggt ttagcttgta
    14581 tttaaacaat cactatccgt ttgtatttta ttttaattat tatttattat atgttagcaa
    14641 atttattttt tagtgtcaac tttttctcaa tattttaatt ttaaataaat tatataaatt
    14701 gaatcttcaa agtaatattt ttatcaataa acaaaataaa aaaggtatta ctttctttaa
    14761 gtaataccca tttattttta atttaagtgg tttttcttaa tctcatcaag tagtccatct
    14821 tctaaataaa tttctaatgc acatcctacc attgccttta ctgctttgtg aagtttatca
    14881 taagcttcct cagaatttgc ataatttaga aattcttttt catgaacata taccattgga
    14941 cttatatcta aggctatttc tgtatacata gtagggcaaa cttgacttac attacctata
    15001 tctgtagaac ctactggacc atctttacct tctaatatat ctgttatacc tgcctcaatc
    15061 aaattattct ttgttatctt ttgaagtact ttgttattta ttatgttgtc aaaagaattt
    15121 tcaaagtatc tataagaaag cttagctcct gtcattaact ctgcaccttt tgcacagttt
    15181 ataacttttt tagttacttc atctaaataa cttctttcag cagctcttac gaaaaactta
    15241 caggctgctt tttctggaac tatatttgga gcctctcctc catttgaaac tataccatgt
    15301 attctaacat ctgattttac atgttgtctt agtgcattta tcccagcaaa agtaagttgt
    15361 actgcatcta gtgcattaac tcccatatga gggtgactag ctgcatgtgc tgatacccct
    15421 tcaaaactaa actctaatga atctatcgcc aaagccttac agtttatatt attaaaagct
    15481 tctatatgca tttgataact tacatcaaca tcatcaaaag ctcctgcctt aactaaatca
    15541 cattttcctc ctgttgtttc ttcagctggt gttcctatta gtacaatttt tcctttaaaa
    15601 ttttctttta acttacttaa tactatacaa gctcctagtg tagatgctgc aatccagtta
    15661 tgtccacaag catgagctgg ttctttattt tctccatatc caggaagtgc atcatactca
    15721 gctaaaaatg ctatggtagg tccatcatta tctcctaatt cagctctaaa agcagtctcc
    15781 atattacaat atggataaac tgtatcaaat ccatattcct tcattttttc tactaagtat
    15841 tttgatgata tgtattcttc ttcccctaat tcaggattgt taaatataaa attacaaact
    15901 tcttcaattt cttttccaag tccattctct atctcataag ctctcttctt tatactctct
    15961 atcataattt acctcctact agaatcctag atataatcct attgtcaaaa atgccattac
    16021 aagaattgtt aatactacca ttaaaggaat tacgaatcta acccactttg accaaggaat
    16081 attggctact gctagtgctc ccattaaaac acctgatgta ggtgttatca agttagttac
    16141 acctatacca aattgataag ctgtaactat catttgtctt gatacatcta ctaagtcgcc
    16201 aagtggtgca agtacaggta ttgtaagtga tgcaagccct gatgctgatg gtattaaaaa
    16261 tgctattaat ccttctatta agaaagttaa atttataaat atagtttttg gtaatccatt
    16321 taataaattt gctgcagaat ttaaaattgt atctattata aatccatttt ctgctattat
    16381 tacgattcca cgtgcaaatg ctatacacaa tcctgctgta attaaatctc ctgctccact
    16441 tataaatgaa ttaactatct catcttgttt aagccctgat gctattcctg atattatacc
    16501 tatagctgta aatatcattg caatttcagt tatgtaccat tctagtctta aaactccata
    16561 tatcataatc gccataccta ttgtgaaaat ggctattacc atggcttctc tttttgtaaa
    16621 ttcctttata tttgtatcac tattaaccat taattcttga tttagtgata tatcatgtac
    16681 taaagatttt ttagggtctt ttttaacttt attggcatac atcataacaa atcctataga
    16741 tatagccata tatattataa acataacaac tctaaactct attccagaac ctggtactat
    16801 ttgtgataat gcttgtgcta ttcctacaga gaaaggattc gtagtagctg ctccaaatcc
    16861 agctgttgct cctataaata ctgtggccac tgctgtcaat gaatcatatc ccatactggt
    16921 cataagtgga ataaatatca tataaaaagg tagtgtctct tcactcattc caaacgtcgt
    16981 cccacctagt ccaaatagaa tcattgaaat cggtattatt aaaaattgaa acttcttcat
    17041 tttatttaca gtatgagcta taacagcttc tatagctcct gttttattaa ctatcccaaa
    17101 agctcctcca actattaaaa caaatccaac tacttcagct gcatctataa acccgtttat
    17161 aggtgctgta aatacatcag taattccttg tggatttgat ggtgcttttt cataagttcc
    17221 agctacaaca actgaccttc cgtctatatc tactctttca aaatttccac ttggcactat
    17281 ccaagtaaga attgccatta acactattag caaaaatatt attgtaagtg tctgtggcat
    17341 agtaaacttt ttctttttta aactgctcat ttttaatctc cccacttttt aaattaaata
    17401 ttttattatt gcatcccttt atcctatttt gtatagatgt aaaaaagctc aacatggata
    17461 catgttgagc taaatgtgct aaatacaaca tacctgcatc ttccctttat cttgtttaag
    17521 atagctctcc atcaaaattc gggataattt tgatgacagt actgtaccta ttaaatacag
    17581 tcccagcact agcttcggac actagcactt cggcacttac tccttttata ttgcctcatt
    17641 ggtttccaac ctcttcaata ttaatcttag taaatgcaac ctctacctca atctgtttgt
    17701 tagatgaggt aattcttatt aatttttatt aattcaaagt aattgtagca taatatgtcg
    17761 agattgtaaa tattttcagt ataataattc ttactttatg atttcattcc atttttcaca
    17821 aataattttt tctccatata atttaaatac tgtagtatat tttaaataca cacaaacttt
    17881 ccacaaattt taaattatta taataccttt ataataaaaa ataaaagcta tgtaaactta
    17941 tcaatttaca tagctttata tacattaatc tactccaagt acagctttaa gctttgatgc
    18001 tattgtaata actcgtggac caaatataac ttgaactgag tttttctttt taacaactcc
    18061 acttgcaccc aattcattta cccagaagcc atcttcatag aatccatcaa ttgaatttag
    18121 ctctacccta agtctagttg cacagtttgt aacatttttt atattattac ttcctcctaa
    18181 accttctatt atagaatgta caagctttgt atctttatca tctacttcct cttctactgc
    18241 attgttgtat gctgttttac cagcatctac agattgtctt tctctatatt ctttcttact
    18301 ataaagctta acttcttctg agcttcttcc tggtgttttt atatcaaact tcttgattgc
    18361 ccatgtaaat attatataag taactataaa aaatattggt atcaaaaata ataatggtaa
    18421 cgcatccact ttttgtggtt gtaataaatt aggaaacata tttctaaatg attctcccat
    18481 tatagaaaca ttaaacattt ctgtaaatac atacgtcaat cctgccagtg gtacatatgc
    18541 tacaaaatat aataatggtg caacaaatag gaatgtatac tctattggtt ccgttacccc
    18601 taccaacatg gctgttacta ttgctggtat caatatactt attgcttttt tcttattagc
    18661 attatcagcc aacttataca tagctaaagc tgctgctggt aatattgcaa aatgtattaa
    18721 taaacgacct gttgtgaagt tacgagttat ataagatagt gtatctgggt ctcccatttg
    18781 agctaatctt atattattaa caccctcgta aattgctcca cctacttcca ttgttccacc
    18841 tactcttgta tattctatag ggaatgcaat taagtgatgt ataccaaatg gtaataatgc
    18901 tttatccatt gctccaaaag taaatgttcc taataatcca ctagaaccta tccaattacc
    18961 aagtttagat aatgccaacc ctactggtgg ccatataaag tacattgctg tacctaatac
    19021 tattgatgct ataaacatca taatcattga aaatcttgga cctgagaaaa aagataatgc
    19081 actaggaagt tctatgtcaa caaatttgtt atgtactgca ctagctacaa atccagctat
    19141 tatagctcca aatatactac aatcatatga aaagattcct aagaaagaac catataaaga
    19201 acttcgacta tatgcttctg cctctcctaa tccagatgcc ataaacgctt ctgctgtaac
    19261 tgtatctgaa tttataccat ttgctgctaa caatgtactt ataactgtgt gcattcctat
    19321 aaataaaaca attcctccta atgcacccca tcctttttcc tttttagata atccaaaggc
    19381 tatacctgta acaaagaata ttggtaagta tctcattacc ataaatccta aatcgtttat
    19441 tactttaaag aatacattta taaatgaccc ttcttttatt agtccgctaa gccctaatgc
    19501 agaaacgttt tccatatttg caaaagctga ccctaatcct ataaatactc ctgctacaac
    19561 aagtaatata acaggtatca tcattgctcc agaaagtact tgtaatttat ccttcatagt
    19621 attatttcct ccatttttat tgaaatgcca ggctatataa tgcatatata tatacttcta
    19681 ttgctttgta taatgtatct atacttatat tttcatttgc ctgatgtgaa tttggattat
    19741 ccccctctat tgtgggtcca aatgcaactc cattttttaa cactcttgca taagatgcag
    19801 ctccttttgt aaaaagttca gcttcttttc caaaaccttg tttatatgca tttgataatt
    19861 tatttattaa ttcactatct ggttttacat acagtaattt taaatcctta tatatatcaa
    19921 ctattaaatt ttcttttttt ccaatttcat taattctatt agttataaac tcccaactta
    19981 tacccttagg atatctaaag tctattttca tattcaattc atttttctct aagaagatac
    20041 tcattataca tattgtagaa acccccatat ctacatcttc tttatatatc cctaattttt
    20101 ttccatgatt atcatctgta aagtatgaat ttaaaatatc ccttattata acgcctttat
    20161 catttagttt tttaattttc tctaaatctt ttgctaaact aaatgcacag ttatatgacc
    20221 tgtgtggatt tctcgataaa gccctttctc ctgtatatct tactaatact ttaccttctt
    20281 ctagttcttc tatctctgta taataagcta atgattctac taaatctttc ttatcattag
    20341 ttttaaaaac agcctcaatt ttgttacata caaatccatc ttctttgttt gactttatat
    20401 caactaaatt gtgatttcta aatttatctt tatctatttt ctttcttaga ttgaagtata
    20461 aaataccttt ttctccattt actattggga agtttccatc tggagaaaat gcaaattttg
    20521 gctgttcatt gacactaaag taatgctcca tacattccca agtcgtctct tcagctccac
    20581 ccactatcaa tctaatcttt ctcttcggct tttcattaag ttctcttaag aaaagtaatg
    20641 catataaaat ccctatcaaa ggtcctttgt tgtcattaac tcctctacca taaagatagt
    20701 tatctttttg acatacttta aaaggtttac tcttccaaag ttctttatta tatattggca
    20761 ccacatctat atgattaagc acccctacta cttcttctcc ttctccatat tctatatgaa
    20821 ttgcatatcc atcaaagtct tttacaacaa aatctttgtc ttttgctatt tgaataagct
    20881 tatcgaaggc atttcttatt tctatcccaa atggttgatt tatatctgtt gtactttcat
    20941 ctctaattga aggtatttca actaaacttc taatatcatt tatgatattt tcttgattgg
    21001 attttaaata atcaataatt agttctttgt ttactatcat cttaattatt tatcacccct
    21061 ttatattaat tttacaaagc atttcagttc atatctacat tataaatttt gaatattatt
    21121 ttttcaatat ttcaaatact tctctaagtt accataatga tacggtaaac aatcaacaga
    21181 ttatgacaaa atcatactat acctattact aatattttaa cttatctatt acgtcttttt
    21241 taatgtaagt ttattccaaa aatacataaa aaaagatgta gccttagcta catcttctta
    21301 tatctattca acttccatta ttatgctatt aatatctaca tttccaggtt ctatcctatc
    21361 ttttactgtt ctcttaacag aatcagcatc atccattata actacaggag ttattatatt
    21421 taaatcattt gacgttataa aatctaaatc tacttccatt attaaatctt tagcttttac
    21481 cttatctcct gcctttacct taacatcaaa cggctttcct tctagtgaca ttgtctctaa
    21541 acctacatgt attaatatat ttgtattatc attactctta atcattattg catgctttgt
    21601 atcaaataca ttgactattt caccatcaac aggagaatat aacttacctt cacttggtat
    21661 tattgctact ccatctccca tcatcttttg ggcaaaaact gcatctggaa catcttctaa
    21721 gttcactaaa tttcccttaa aagggctata tattttctca atcttatttt cttttttctt
    21781 aaatatatta aacataacac actctccaca ttataaaatt ttatgataat atttttctta
    21841 aagcttttgc tacaccatca tttacattag tatcagttac aaaatctgcc atagatttta
    21901 cttcatctgt agcatttccc atagcaatac caagacctgc aaatttaatc atggatatat
    21961 cattttcatt atcacctata caaataactt cttcgctatc tatacctaat atatcacaaa
    22021 attttttaac tgcaatacct tttgaagtac ctttgctcat aacttcaaaa tttatctttc
    22081 cagaacttac tacttctaaa tcttcttgtt ttttaaattc ttcctttgct tctctcaaag
    22141 aatctacgtt ttctgagaaa agtatacatt ttaaaatttc attatcagct ttttctaata
    22201 aagtttcaca acttgcattt tctattattt caattctgtc ttcttcttta tttttactat
    22261 ttattaatct atatggatgt tcttcaccta tttcattgtt ggatataact attttacagc
    22321 ctttaaaatg tttgtttaaa ttatactttt cacctattgt atagattttc tttagagttt
    22381 ctttgctcaa aaccttctta tactcatatc ccagtaattt aaaatatgtc ccattagttg
    22441 atataatata tatatcatcc cctaaaacac ttgtaaaata tttcatagca ttataaggtc
    22501 ttcccgtaac taaggctacc ttgataccct tttccatgac ttcttttagt gctttaatat
    22561 tttcttctga tacttcaaat cctttaccca tcaaagtacc atccatatca gtacaaatta
    22621 atttatatcg catttgtatc acatcctagt aagatttttc taatatttca acaaggtctt
    22681 ctttctttcc aaaaggtaaa aatcctaaaa ctggacagct tgttatttgt tctaattgtt
    22741 cctttgttac attaaactca gacatttttg tcttaagtcc cactctcttt aaaaaacttt
    22801 ctatttcatt tcctaaagtt ttagctgctt catcatcttc cactttttct aattcttggt
    22861 ttagcattct cgctactttg gcaaatttct ctatattatc cttatagaac ttttctgcaa
    22921 attttgggaa cactatagcc aaagcctcac catgaggaat atgtgtaact cctcctatta
    22981 tttcacttag tggatgtggt gctgctgctc cagcatttgc cagtgaactt cctcctaatg
    23041 tatctgctac tgctaacttt gttctgtatt ctatattatt tttatcttcc attactttag
    23101 gtagatagtt aatgactaat tccatagatt tttcagacat tatctctgta aaatcattag
    23161 catttggatt tatataactt tcaaatgcat gggtaaaagc atcaaatgct gttgatgcag
    23221 taattctctc tggcagagtt aacataagct ctgggtcaac tatacattct tttgagaaac
    23281 aattttgatg aaatattgta tttttctcgc ttcctctcga tattactgct gcttgtgtaa
    23341 cttggcttcc tgttccagaa gtagttgata cagctattaa cggtaattct ttttcactta
    23401 ccttatcgta tattttaaat ggatttgtat atgtactaaa catataatcc caatctatct
    23461 tttctaaacc aaaagtcaat gctaatactt ttgcagtatc tatacttgaa cctcctccaa
    23521 cagctaaaac aacatctaca gattcttttt tagctaattc aaacccttca tttattaatt
    23581 ctacagttgg gtttggctct actctatcaa agtgtacaac ttctatacct ttttctatta
    23641 aattcaatgt aactctttta tataacttgt ctaatggctc tacatttgga gtagtaacta
    23701 acaaacattt ctttccatat tttcctatta ttctaggtaa ttcacttaaa gttccttgtc
    23761 caaaatttat tcttgatggt tgataaaagt taatcatatt tttcctccct taaattattg
    23821 ccttcatatt caaattatac atatcatagg atttttaagg caataccttc actgttaact
    23881 cttatttact aaagcttatg gcaattatta gacaataaat gttttattat taccatatca
    23941 ttatgtaaat tttattaagt acccatgtta taattttata atcaatctta ttgtataatt
    24001 gacttaataa taaaaataga gtatatacac ttttttatga caaatagctt aaaggagaat
    24061 taaattatga aatatttgat agacagtgct aatcttgatg aaattcaaaa gtgtttaaag
    24121 tatggattta agggaataac agctaatcca tctatgtatc ttaaagagaa agtaaacttc
    24181 catgaattta ttgagacatg tgctaattta aatccagaaa tattaactgc tgaagtcatt
    24241 ggaaataatt tatacgaatt aattaattca tgtgataaga tattaaatat aaataaaaac
    24301 actataataa aaataaattt ctctgaagtt ggcttagaac ttataaacat attaaatagt
    24361 aaaggtgtaa aaactgcatg taccttgatt ttcaatgtaa atcaagcatc attagctata
    24421 aatgcaggtg ctgattacct attccctttt ataggtagaa atgatgaaaa tgggaatgat
    24481 ggtataaaaa accttacaga aatatgtaac cttatatcaa taaatggata taatactaaa
    24541 gttgttgcag cttctattaa aaatgtattc cacttaactc aagcatctct ttgtggatgt
    24601 gattatgcag ctgtaacata tgatttattt aaaaaagctt cttttaacca gcttacaata
    24661 gatggtgcca atacttttga aagagattgg tccaaggtta ataaatagta actaagattt
    24721 aggagggttt gcattgaaca aaagattatt agatattgct gaattattac tcaacagctc
    24781 agactatatt actgtagata ctattgccga agaattaaaa gttagtaata aaactattcg
    24841 taatgacctt gttattttag atgaatggct tttagagttt aatttatccc tagataaaaa
    24901 aactggttca ggggttatta ttctaggtaa tgaagatata aaacttaaag ttattagaga
    24961 tatcaatgat aaatcaaatt gtatatatgc atactctcct gaagatagaa aaaggtatat
    25021 tctaagtaaa ttatttatta aaaattctaa gtttaggatt cgtgatattt gcaatgaact
    25081 acatgttagt agagctactg tgcataaaga cttggtagtt attcaaaatt ttttgcaaaa
    25141 ctttaaagtg actctagtta gaaaaaccaa taatggagta tatttagagg gaaaagagaa
    25201 agatattcga aaagcaattt ttgaacttgc tactgttaat aaaagttata ctgaacttaa
    25261 agaaatatta ttctcgaaat ctaatgaatg caaagatacc ccatcaacaa aaatatttaa
    25321 ggagctattt aactatgatt ttgagaaatt atctcaaatc actctacaca ccttacaagt
    25381 agaaaaaagt aaccttagtg atgagtatta tataaacttc ttaatacata tagctatatg
    25441 tattaaaaga atttcaatta aaagatatat taacttatct gaaggttttt ttagagaatt
    25501 attagaacat gaatactttg aaaaatcaaa aattttatgt gctaacctaa gtgaagcttt
    25561 tgacgtagaa tttattgatg aagaggtctg ttacatcctt cttcatatag aaggattact
    25621 taaatcaata aagaaaacta ctactcttga acctcttaaa aatgaagaaa ttacagagta
    25681 taatctatct gaagcaatag ctgttcattg gggaagtata ctaaatttaa acttaaagga
    25741 cgatttaata ttaataaagt ctcttgctaa tcaccttaaa tcagttctac acagaataaa
    25801 ctatgggttt actattacaa atcccatatt aaatgatata aagagaacat ttccttatac
    25861 ttataaggct gctaaagagt ctaatactgt tatcaaacag ttgatgaatt atgaagtcaa
    25921 tgatgacgaa ataggatatc ttgctttata tttaatatca gcaatagata gaagcaaatc
    25981 tcccctaaat actattttaa tatgtcactg tagtgatggt gtatctaatt tattagtcca
    26041 aaagttaagt tttgaattta atcagattaa tatagttaaa tctatacctc tatcttctat
    26101 ttcatttacg aactttgatg atgtggactt aatactaact actgctcctg tagactttga
    26161 acataatgct gaactgataa atataaatgc tcttttaagt aagaatgata tttctagact
    26221 tagtacaatt ataaaaaaac tatattctga aaaaaataaa atattatctt ataaataaac
    26281 aaaaataggc ttgtctcaac atagagataa acctattttt cattgtccaa atgataaaaa
    26341 tatatttgat ttcatagaaa aagtgcctca tgaactaaaa aattcatttt gagacacctt
    26401 cattttttat atttaatctt tttatttaag cttttatatt ttttaaactt ttatatttaa
    26461 agtaaatttt attattttta ttgagcacaa gcattattta tattattaat ttcttcagta
    26521 ttcttaccaa actttgtagt tacaaatcct attgttttta ttatgagtaa taaggatatc
    26581 aatgaatata tagatatcat aaaattctta cttattattc cagacgctac tattataaat
    26641 ccatctagca taaataatat aactggcaac ttcagaaact taattacctt attaataagc
    26701 attgcaacta aatcagttcc accagtacta cagcccctta ttattgctat ccctatagca
    26761 gttcctacca ataacccacc tatcactcct gctactaata gattgtcagt tatgtgagtt
    26821 tgtggtatta cttttaagaa taatgggccc ataagagtta tatataatgt ttttattcca
    26881 aaacctttcc ctaaaaataa taatcccatt actaaaagtg gcatagatat tcctaatgat
    26941 atattctcaa caggcattga taagaatgtg ctaagtacga ctgataatcc tgttatacct
    27001 cctggagcta atgtatgttc tgcaaagaaa aaattaactg ctaatgccat taaaaatgct
    27061 gatattgtta tcagtaaata atcttttatc atttcttttt tcatttaata acctcctatg
    27121 attatattgt ttcagtataa aaaatcaatt aaattagtct agcttcctgt agtttttcat
    27181 aaaattaatg cttccttaaa acattaagaa agcatctctt ttatcatagt ttattatctt
    27241 attctaaacc aaactttgta gctgcttcat ctatagcttt tctacaattc ttaacacctg
    27301 ctgttatacc atctttatcc ttgaatattg ctgaagataa tattacaacg tctgggttac
    27361 attctattaa tggctccata ttgtctgatc ttagtccacc atctattgct aattcacact
    27421 taggattctt ttcgtctatc atttttctag ctctttttac taaatcaaca gcactctttc
    27481 tccatcccca gttatctttt ccatcagttt catcaacacc atgaacgact atttgcaatc
    27541 tgtctatatc atatatagct tcatcaacaa aacataatgg tgtgtaacat ccaattgtta
    27601 atcctacttt cataccaaat tctctacagt aatttattat gtatgcaaga ggagcaccta
    27661 aaaaatgttc tgctggtatt atcaacaagt tacatcctgc tgctgctatt ttttctataa
    27721 ataatctatc acaatctctt gtgtatatat ggcattctat tggtttatct gtcactggtc
    27781 ttattgcttc tattatttga tgaccaccca taagttgcat attttttaag tcatgcatat
    27841 ctgctgcatc tgaatgtacg tagtctacac ctgcatccat tgcttcttta actacggctg
    27901 atatatgacc gtaatttaca tgtgctaatc ctgctgctat ttttatgtgt ttcatatttg
    27961 tatacctcca tattttactt taattagtat taaatattag tttttgatat ttttttaata
    28021 ttttttaatc cagtattact tttatttagc attactttta ttgctgtata tatttctttt
    28081 atgaacttat tctgcttttg ttaactgtga acttattttg ctttgtaaat taatattaca
    28141 ctgatatatt ttttttcaca acactttgac tactccttaa aagtaccttt atattgtggt
    28201 aaattataga cattaattgt atataaaaag ttagtttttt accacattct tatggcattt
    28261 attgtctata ctaactcaat tgatatagct agttcattta atatataatc agactataaa
    28321 tataaattta ctcgataaaa agtaccattc ataacaatat taatgaaagg gagatgtaaa
    28381 atgagaaact gtattattgc ccaatctgga ggccctacag ctgttatcaa tgcttctgca
    28441 gttggtattt atgaaaaaaa tttagaaact aaatactttg ataaagtata ttttggtatc
    28501 aatgggattg aaggtatatt aaacaaaaat ataatagatt cagactcctt aaacaaagat
    28561 aatgtattaa atttaaagca aacaccttcg tctggtcttg gttcttgtag atacaaactg
    28621 gctgattata aaataaattc ttatgattat gatagagtta taaatatact agatgaatat
    28681 aaaatagata cattatttta tataggcgga aatgattcta tggacacagt gatgaaattt
    28741 tctcaatact gcaaaaacac tacttgtaaa ataaaattta taggtattcc taaaacaata
    28801 gataatgatt tattaggaac tgacaactgt cctggatttg gttctgctgc aaagcttatt
    28861 tctacactaa taatggaaac atacttagat ttttctatat acaaaaataa tggtatattt
    28921 atagtagaaa ctatgggtag agatactgga tggttagcag cttcttcttc actagctaga
    28981 cttaatgata ctccacttgt tgatttaata taccttccag aaattgattt ctatgaagat
    29041 aagtttatag aagatgttag aaaaatattt aaagaaaaaa ataaggctta tatagttgca
    29101 tcagaaggta taagggataa atacggcaac tttatatgcg aacaaaaaac ttctgctcat
    29161 gataaatttg gtcattccca attaggtgga gtaagcaact atctaaagaa tcttatagta
    29221 tcatcatcaa ttacaacaag agtaaaaacc ttagaacttg gtgtaataca aagatgtgct
    29281 atgcatttag cttcagatac tgatttaaaa caagcagctg aagttggtgc atatgggata
    29341 gaactatcta aaaaaggata tacaggagtt atggctatca ttaatagaac atgcaataat
    29401 ccttataagt atgaagttag tcatactgat atttctaaaa tagctaacaa gacaaaatat
    29461 tttcctaaag agtggataaa tgaagaatgt aattatataa ctaatgaagc cctaaattat
    29521 ctatatccac ttattcaagg taaaccagat atcaaatata aaaatggact accttatttt
    29581 tcaattattt actaggaaat tattataatt tgatatacac tcaattctat cgctcttaaa
    29641 tacctaagtg gctgttaaat aaaattataa attgttatat gacagtcact taatcgttta
    29701 attatttaac tgtttagttt tttaattttt taatcttttt aatactaata caatttgttg
    29761 taataatatc tcacacccat tttcattttg gatagattat aattagttag acaataaata
    29821 ttttgtcatg atattattat atatagtgat acaaatagaa atacttttat catggctaga
    29881 aaaataaaga ttacacaaag gatttaaaag tcaaacaact tcaattaaaa taaatggtta
    29941 aaataacttc taaatcaact acataaacaa taaaagaaca taaagtagtt aaatctacaa
    30001 ttataaaatt agtaagacta taataactta aataaaatta aagttaacaa agactat
//
LOCUS       DAELNY010000030        29066 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.030, whole genome shotgun sequence.
ACCESSION   DAELNY010000030 DAELNY010000000
VERSION     DAELNY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.030"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            38..724
                     /locus_tag="KPE55_001152"
     CDS             38..724
                     /locus_tag="KPE55_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0492247.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            714..1745
                     /locus_tag="KPE55_001153"
     CDS             714..1745
                     /locus_tag="KPE55_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0492248.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITADLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            1827..2492
                     /locus_tag="KPE55_001154"
     CDS             1827..2492
                     /locus_tag="KPE55_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492249.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            2505..4922
                     /locus_tag="KPE55_001155"
     CDS             2505..4922
                     /locus_tag="KPE55_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492250.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTTIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNLVQMYAKLKSLGMTSFQLKKIIFLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKVKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKLIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTEGMFFASDKTLESLTPYNTN
                     LDFSILIDKKYSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(5103..5894)
                     /locus_tag="KPE55_001156"
     CDS             complement(5103..5894)
                     /locus_tag="KPE55_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0492251.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5927..6514)
                     /locus_tag="KPE55_001157"
     CDS             complement(5927..6514)
                     /locus_tag="KPE55_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0492252.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(6690..8081)
                     /locus_tag="KPE55_001158"
     CDS             complement(6690..8081)
                     /locus_tag="KPE55_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG0492253.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSREIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKIGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(8271..9569)
                     /locus_tag="KPE55_001159"
     CDS             complement(8271..9569)
                     /locus_tag="KPE55_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG0492254.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(9928..10803)
                     /locus_tag="KPE55_001160"
     CDS             complement(9928..10803)
                     /locus_tag="KPE55_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG0492255.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(10996..11682)
                     /locus_tag="KPE55_001161"
     CDS             complement(10996..11682)
                     /locus_tag="KPE55_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG0492256.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(11731..12264)
                     /locus_tag="KPE55_001162"
     CDS             complement(11731..12264)
                     /locus_tag="KPE55_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0492257.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(12308..12940)
                     /locus_tag="KPE55_001163"
     CDS             complement(12308..12940)
                     /locus_tag="KPE55_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG0492258.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(13030..14109)
                     /locus_tag="KPE55_001164"
     CDS             complement(13030..14109)
                     /locus_tag="KPE55_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0492259.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(14242..15588)
                     /gene="pepV"
                     /locus_tag="KPE55_001165"
     CDS             complement(14242..15588)
                     /gene="pepV"
                     /locus_tag="KPE55_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG0492260.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(15657..16937)
                     /locus_tag="KPE55_001166"
     CDS             complement(15657..16937)
                     /locus_tag="KPE55_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG0492261.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKSKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(17064..17324)
                     /gene="hfq"
                     /locus_tag="KPE55_001167"
     CDS             complement(17064..17324)
                     /gene="hfq"
                     /locus_tag="KPE55_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG0492262.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(17438..18373)
                     /gene="miaA"
                     /locus_tag="KPE55_001168"
     CDS             complement(17438..18373)
                     /gene="miaA"
                     /locus_tag="KPE55_001168"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG0492263.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(18411..20378)
                     /gene="mutL"
                     /locus_tag="KPE55_001169"
     CDS             complement(18411..20378)
                     /gene="mutL"
                     /locus_tag="KPE55_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG0492264.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(20389..23232)
                     /gene="mutS"
                     /locus_tag="KPE55_001170"
     CDS             complement(20389..23232)
                     /gene="mutS"
                     /locus_tag="KPE55_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG0492265.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(23393..24127)
                     /locus_tag="KPE55_001171"
     CDS             complement(23393..24127)
                     /locus_tag="KPE55_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492266.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(24096..25109)
                     /locus_tag="KPE55_001172"
     CDS             complement(24096..25109)
                     /locus_tag="KPE55_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0492267.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(25131..25901)
                     /locus_tag="KPE55_001173"
     CDS             complement(25131..25901)
                     /locus_tag="KPE55_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492268.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(25876..26166)
                     /locus_tag="KPE55_001174"
     CDS             complement(25876..26166)
                     /locus_tag="KPE55_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG0492269.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(26297..26396)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      27016..27105
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            27158..27334
                     /locus_tag="KPE55_001175"
     CDS             27158..27334
                     /locus_tag="KPE55_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492270.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(27449..27805)
                     /locus_tag="KPE55_001176"
     CDS             complement(27449..27805)
                     /locus_tag="KPE55_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492271.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            28061..28974
                     /locus_tag="KPE55_001177"
                     /pseudo
     CDS             28061..28974
                     /locus_tag="KPE55_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT         9819 a         4666 c         3260 g        11321 t
ORIGIN      
        1 tgacattata aaatattaaa ctaaaaggag taactgtatg gaaaaaatat tagtagtaga
       61 agatgattct atattaaata aaactctatc atataaccta attgaagatg gatatataat
      121 tacttcaaaa tttacagcta aatctgcact gaagtctatt tttgaatgtg aatttgattt
      181 aataattttg gatattaatt taccagataa aagtgggttt gaattatgta atgaaataaa
      241 gggaaattac aatataccaa taattttttt aactgctaat gatatggaat gtgatatgat
      301 taaaggctat gaacttggag ctttggatta tataacaaaa ccatttaata tcaatatatt
      361 taaacagaaa gttaaagctt ttttaaatca tcttacaata aaaacaaaac aagattacta
      421 tagggatgga tatcttgaaa ttaacttttc tgagttatct gccaatataa atggaaatca
      481 aattatattt acaccattag aatatcgtac tttaaaactg cttactgaaa atcctaaaag
      541 tattttaaca aggaaagtac ttttagaaaa actctgggat atagatgcta actttgtaga
      601 tgaacatact ttaacatctg ttataagtag gattagaagc aaaattgaaa aagacaatct
      661 taagtatatc aaaacggtat atggtatggg atatatgtgg ttaggtgaaa gaaatgaatt
      721 ttaaaaaatt ttcttttagt tctatttatt taataattgc tgttggtata atttttacag
      781 ctattttatt tagtatctca atttatctaa taactgctga tttaaaacta attgctctat
      841 tattaatata tacaatagtg ctttgtggat ttatagcatt aataatattt ttacttcata
      901 aaaaaatagt tatattttgt tctaaaatat gtcacacttt agatagtatg atgaatagtg
      961 aagacaatat tgaaattgaa ttagaagaag aaactatttt atcacgtatc aatcacagat
     1021 taattagaat gtatgaagtt attcaagaaa atcgcaatag tattgctata gagaaagctg
     1081 atttacaaga actagtttct gatatatctc atcaaattaa aactcctatt gcaaatttaa
     1141 aaatgattaa tactactcta ctaaatcaaa catttaaaac agaaattcaa aaggattttt
     1201 taatggatat ggaaaaccaa ttagataaat tagatttttt aatgcaatct atggtaaaaa
     1261 cctccagact agagacaggt ataatcacac tatctaaaaa gaaaaattca atttatgaaa
     1321 caattgctac agcacttagt ggaattttgt ttgatgctga aaaaaagaat attgaagtta
     1381 cagttgattg tgacccaaat ttatatataa ttcatgacaa gaaatggact tctgaagcta
     1441 tttttaatat attagataat gctgtaaaat acacaaattc taatggtaaa atacgtgtag
     1501 ctacagaatg ttgggttatg tatacaaaaa tagatattat tgataatgga aaaggaatat
     1561 cagaaagtca tcaagcagaa atctttaaaa gattttatcg tgaagaagat gtccatgaca
     1621 tagaaggtat tggaataggt ctctatcttt caagaaaaat tatatctctt caaggtggct
     1681 atattaaagt tacatcagat attggaaaag ggtcgacttt ttctattttc ttacctaata
     1741 aataaatttt taaatatttc atttgtaatg attctgcaac attttggttt tatactgtat
     1801 ttacaagatg ttaaggaggt attattatga gtattttaca agtaattaat ttaaagaaat
     1861 attatggaca aagttcaaac attacaaaag ctcttgatgg tgtaactcta tctattgaaa
     1921 aaggcgaatt tatttctatt gtaggtacat ctggtagtgg caaatccaca ttattaaata
     1981 tgatgggagg attagataat cctacatctg gaaaggttat tataaagggt aaggaattat
     2041 ctactatgac tgatgaacag cttacaatct ttagacgcag aaatataggt tttatatttc
     2101 aaaattataa tcttgttcct ttacttaatg tatatgaaaa tattatctta ccaataggat
     2161 tggatggaga gaccatagat aaggatttta taaatgaaat tataaatatg ctttctcttg
     2221 aagaaaaatt atatgatatg ccaagtaatc tttctggtgg tcaacagcaa cgtgttgcta
     2281 tagctagagc acttgtaaca aaaccagcta ttatactagc tgatgaacca acaggtaacc
     2341 ttgatagtag aactagttca gatgtactga gtttacttaa agtgacaagt aaaaaattca
     2401 accaaacaat tgtaatgatt acacacaata atgaaattgc acaactaact gatagaatta
     2461 ttcgtattga agatggaaaa attgttaact aggaggtgta gtatatgttt agaaataata
     2521 atcgcacatt tataagaaaa atagctttaa atgatttaaa aattaataaa cttaaaacat
     2581 atctttcagg tatcattata atgatttcta catgtttatt attaacagtg accttagttt
     2641 catataatgc ctctgtagat ttagttaatg catctccata tcatgctata tataaatctg
     2701 tagatgaaaa agcaaaaaat atactatata aaaataaaaa ctttagtggc gtaggaactt
     2761 acaaattaat tggaagtaat aaaaagacag attgtattat gtcgattgtt tatgcagatg
     2821 atacaacaat taaacttatg aattttcaac cattgaaagg caaactacct acaaataaaa
     2881 atgaaattgc tatttcagaa aaatatttac aggaatttgg tttagataaa gatattggag
     2941 atagcattaa attaaattat tatgatgata ttacgaataa agaagttcaa tgtgatttta
     3001 ttattgttgg atttctagaa aattattatc aagataatgc caagcaatac tatacagtcg
     3061 tttcaaatga ttattttaag aatatagctt ctacaccttt acaaaataaa agtaatactt
     3121 ttgacgagtc aagacctgat actgtagatg tattagttaa actatcagaa gaaaatacag
     3181 agaaagattc agcttcaatt aagacacagt taaagaaaat tggtttatct ttaggtatta
     3241 agaaatataa tatatactta aatgataact atattgaatc aaatttaatt gatggtgagc
     3301 agattattac agttttagta gttggaatag ttgttctttt ttctagtgtt tttgttatat
     3361 atagtatttt ttatatttca gttgttaatt tagttcagat gtatgctaag ttgaaatcgt
     3421 taggtatgac ttctttccag ttaaaaaaga ttatttttct acaaggaaat attttgtcaa
     3481 ttatttttat tccattaggg gttatagctt cttgtataat tgcttacata attcaaccat
     3541 tagcatggca aatgatagct gatttattta taattttaat tttatcactg gtaatgtttt
     3601 taactgttag aatttcttta tttaaaccag ctagaattat ttctaaaata tctgctattg
     3661 aagccatgca atatacagaa actaaattta gaaaaaaagt aaaaaatttt agttatataa
     3721 atataaaaaa tctagcatta aagaatatcg agtcaaatag aaagaaaaat ttgattgcat
     3781 taatatcgct aagtattagt ggagttttat ttatttcaat cgctaatctt gctaattcaa
     3841 tagatttcaa aaaacaacta tctcaacaat ttgtctataa cgaagattat ataattagca
     3901 ttaagagtga taacttatat gagcatatta cagaaatcca acaaaataat ccactaacag
     3961 attctcttaa gaatgaaatt aaatctattc atggagtaaa aaaattaatt gaatccaaat
     4021 taattaaaag taatattata gaaccttaca tcaaagatag agacgaaaaa caatttatta
     4081 ctctaattaa aggtattacg cctgaacttt caaaatcact tgaagaatat attgtcagtg
     4141 gaaaaataga ctatacacat ttagattcag atagtttaat tataaataaa tatagaacaa
     4201 aatattatgg attaaattta aaagttggag atacagttac atttaatatc tttagtggaa
     4261 atgatattat taaaaagaaa atgaaaatta taggaatcat tgacaattcc aatacagaag
     4321 gtatgttttt tgcttcagac aaaactttag aatctctaac accatataat acaaatttag
     4381 atttttctat tttaatagat aaaaagtatt ctaaagaagt tgaagaaaag ttgttaagta
     4441 taattcagaa aaattcaaat ttaatgcttt attcatatga agatgattat agaatgatta
     4501 ctagagcatt tcaatatata attatagctt catacatttt tgttggttta atttcttgct
     4561 ttggtatact aaatatgacc aataccctaa taaacagcgt tttaattaga aaaaaagagt
     4621 ttgccttact tcaagccgtt ggaatgacaa gaaagcaact gcgaaatatg ctttatagag
     4681 aaggtctaaa cataagtatc aaagctatat gtacttcaag tatactaggt tattttggtt
     4741 caaatctact ttgcactttt ataaaagatg taattagatt agactttatt aattttaaat
     4801 tttctatatt tactatttta attttttcat tcgtattaat tggtatacaa gttttagtga
     4861 ctgaattact tgtaagaaat atagaaaaaa aatcacttac agaacgctta cgttctcagt
     4921 aagaatgtat ataaaaaata gccatttttt atacaagttt taacataatt ttttaaaagc
     4981 taaaaggata gaaaattttg actttacata attttctatc cctatacttg tttttatttt
     5041 atacagatac acttccttat atctccgagc aacatcacca gagacataaa caagcatatt
     5101 ccttattttc taaatatgtc aattgcatga cttgtaaccc ctactaaatc ttcacgttca
     5161 caagtagcaa ggtgatattt taagtataat gcaaatgcat catcatccat agagtctact
     5221 gcttcacgca tatacaaagc caaaccatct gatgcgacat aatgcagtct agttacatta
     5281 aatgcagaca tcaaatcatc aatgttttct ttacgtacaa gctcaaacaa atcctttggt
     5341 tcagacttag cagcaaatgt ctgtggatca atcaaacctt tttcaatata ttcagataca
     5401 ttaatatttc ctctatggaa cccctcgtca atgagacagc catcagatat aacatatgca
     5461 gcaaaaacca caccaccagg ttttgttaca cgaattgcct catgtaaagc ttgttgttta
     5521 tcttctttgt tataaagatg atatagtggt cctaacagta acgtaatatc atatttatta
     5581 tctgggaaaa cagataaatc catagcatta ccctgagtaa tgcttatatt ttcatttgat
     5641 tgagtatttt tatggaaaac ctctatatta tgttcgacta attccactgc gtcaacatca
     5701 tatccctgac gtgccagtgt gtgagaataa cgaccagttg cagcgccaat ttcaagcaca
     5761 caatcacctg attttatgta cttatcaatg taatgcatag tagtgagaaa ttccaccatt
     5821 ccatgtttta atgcaagtcg actatcttca tcataagtat tataaaagtc aattaagtat
     5881 tgttttgttt ccataatttc acctcttcta tattagttaa taaacatcaa atagacaaat
     5941 tccaatctgg tttttcttgt cttataaaag ctgttaatat atttttcaac attttttcag
     6001 cactatatct atctaatgtt agtttttttg ttgctgttaa gacagctatg ccatgagtat
     6061 aaagaaataa atctaaaaat attacttttg cacattccaa ctctattcca atgctttccg
     6121 aaaaaagtct tgcctttttt tcattgtcaa tttctttata aaaatcattc gcctctttca
     6181 tcatcaaatc taaatcattt ataaataaca acttaaataa atacgtttct tcttgagcaa
     6241 attcaatgta tgaaagcgga agcagtaaat aagggcttac acttttagaa ttacgataat
     6301 taacaacata ctgattataa tattcatatg caaaagcaag aaattcattt tttaattcat
     6361 tcatgttttc gtaacaagta aaaattggtc gagtggaaca ctgtaattta cttgcaatac
     6421 tcctcgcatt tacagcttca aatcccactt ctcttgtaat atccaagact gtattcaaaa
     6481 tcatttcttt tgttatcttt actttaggtg gcatatatgt tcacctctcc attaaatttt
     6541 tataaaacac atatgttata taactatagt aacatatcaa gaaattatgt caagaaaaag
     6601 ctttgcattt ttttgcaatt cctaaaactt ataaataaaa catgagttgc tgttatctat
     6661 taatagataa cagcaactca gattttcctt taaatactct tctttttgtc taatatttca
     6721 tatacacaat cttctattga attaatatct ttttcttttc tttttttaga atcaattaaa
     6781 atgaaataca gtataaaacc tacagcaagt ccccatgttc cgcctttagc agcaattaca
     6841 cctcccataa gtccaatcaa tcccttctca acacctgtat tacatagctc cattgatagc
     6901 tttgtacata aatatccttg aactaaaagt gttactgaca atgctaatgg tataagaggt
     6961 gtagcaatct gcgcaattgg ataaaagcag ataaaaattg ctgaaaccca tactaatgta
     7021 cccataccac taaaaacaga ctgcatggct tcctttccaa tcttgtacct ttgagcaaca
     7081 cttcctgtaa gtgtcgcagc taatggacca cacattggta tatatggaga aaagattgcc
     7141 ataaaaatat ttcttatccc acttataaca ttagacctat ttgaattgaa atctatatat
     7201 tcatcttttc tattttcact ttcagaaagt actaaatttt cacctgttat aaaatcacca
     7261 aatgcaatta tgtatatcat aaaagccatt ggtatccctt gtataaataa cattacactt
     7321 ggaaaaccta ttccaaaagg agatactgta tttatcagat ttttaaaatc tactatgtaa
     7381 actaaattgt caaaactaaa tctaggaatt tctatttctc tactaaatat acctactatt
     7441 aaactcacta aaatagctga tactacacca tattctccta aatgaaataa aaatttattc
     7501 ttaactttta gagtatcaaa tctttcagaa aacattacaa aacaagcaac caaaattcct
     7561 actgttatag atataggata agaatcaaat ctccctttag gattaaactc tgatattata
     7621 gtcgatatac cgacacccat tagtattcca cctttaatag aatttggtat cttactaact
     7681 aactttccag ctacaccagt tatacccatt aaaataaata ttattcctaa atctatttgt
     7741 aaagctgtta atgcttgtat tctttcaatg cccatgctga actttaacaa atatgcagtt
     7801 gtaagagtta tagaagctgt aatccagcca ggtacaactg ggtctcccat aaaaacatgc
     7861 aaacagtata atattgagtt aatgatagcc atactccatg ccacttcata gggtaatccc
     7921 aatatttgtg tactaaggcc aattgtgcct aaacttgtca tacttgatat tataccttga
     7981 atagcttctg gtttctcaaa tctgtaatgt ataaatggaa ttcttatctt aaacattcca
     8041 aattttatat gaggttgttc ccttctagcg agtctttcca taacttcact ccacctttca
     8101 ataactcttg taagtttaca actttataat tgtttataaa ttatcaatgt cattaaaatt
     8161 agtatataac atgcaaatta tataaacaat aaattaaatc tataaaattg atagattttg
     8221 tgcatatata gaaagaccac ttcattttta gttacttaaa tcacttaata ttagttattt
     8281 aaatcacttg ataaaggttt tttataaaat tcaatagttc tattccatga ttcaaactgt
     8341 ccttttgaat ttccttctac agttccacct gaagaaaaaa tccctccact catactcata
     8401 cataaagtct cctttagagg cataatatat ggaattggta acggatgacc catattattg
     8461 tatgttaata attctacgtt atatggataa ttattttctt caaagatttc taatgcctct
     8521 aaacaagctt cataactatt ccatatgtta tcttcttttc cagcaatcat aagtatatct
     8581 gctttggaat tttctatttt tattctagcc tcttttctat tctctgctct tttaagactt
     8641 ttcttataag tactattaaa tccaaatgga atatcttttg atatattttt tttctgttct
     8701 tcaaagaata tatcattgtc tactttgata tatggaaatg gctctccttt atatgaccat
     8761 gatgaagtat catttccact catcaatcca ttaagagctt ggaaacaata aatgtgaggt
     8821 tgtgatacta caaccttttt aatttgttca tatcttgaag caagtaaaaa ggccaattct
     8881 ccaccctttg atgtcccatg tacaaatatt tctttagtac tggtaatttc attttcacta
     8941 atccactcaa atactttttc aaaatattct aatggaacct cttctaattt atctggtaac
     9001 ccttctaaag caaagtatgc aactgttagt acattgaatc ctctagatgc aagtggagct
     9061 gctaaaaggt ctagagcgtc taaatttcca tcagaaccac ctagcattaa aattgtttta
     9121 ttattagaat tttgtttata aaataatttt cctataaact catcagatat gtattttgtg
     9181 gtaatatctt ctgatttaaa tattctcttt aattcaactt cttttctttc aatagaagtt
     9241 tcaaatatca tattcataat tattggttta tcaatagata tattttctgc aaaattttta
     9301 ccttcagttt tagatttttc cagcgaataa attaacccca tacaattatc agccttataa
     9361 gtaccttcta taggttcaac taaatctaaa tctacttctc ctttttcatt tgaacaaaac
     9421 actccataag atgaaaattc ttctcccttg caccatggaa attccatttt cacacttatt
     9481 ttaagtttag tatttggaat taagtccgaa accataatct taagtctttc atccacaaat
     9541 gattctacat tgtatacatt tactttcata atctatttct ccttataata attatttatg
     9601 ttttatataa aacagcttta tctaattcaa cataaataaa tttttaaatt aagacaccat
     9661 attgactatt tcattttatc aacatattcc atctttgtca atatattgtc ttttttagta
     9721 atttaaaatt atgtagtcta tatgaaatcc agttgatttt tggctaatta aagataggaa
     9781 tgctattagg tttaaaatat atctattcaa aataaaaaac cactcatttt aagaatggtt
     9841 tttaatataa actatttgtt attaataagg atattttttt atttaaattt atataatcga
     9901 acatctaata tttaatgttt aataaaatta tgcatttgca aaagtatata tggcattaac
     9961 tcttgctaca tataaattct catcactaca tccaatatat ctagcatatt tttcaatctt
    10021 ttcttttgca ttttcaacat cattttcatc gctctccatt aactcattta tataaatatc
    10081 cattaatgac gctttatatt ctcttatatc atagcctaaa aattcagtca atctctcaat
    10141 ttccttacta aaatctccat cttcttttac caaaaattcg tatacatctt ccctatatgg
    10201 atttaaagtt attatttgtt ttactacttt gcattcgtca gccttcccaa taaaaccatc
    10261 tttataatta ttaaataagg cattagattt ttttatagat tcttcatcaa tataagaatg
    10321 taccgcagtt atacgatttt ctataagggc atctacaaca gcataatgaa ttttaaaaca
    10381 atcattgtat aaataattgc tcattttctt tattgtcgat tctatttcta agaagctcgc
    10441 gtggccattt tttttatttt tatttggtat aataaaagtt ttattcaaat tattaaaaag
    10501 tctcttaaaa tctaaatatt tttcacaata aacactttta aaggtatcga tatctatagt
    10561 tataattcca aaacttacta tagtctcaat tcctttctta aaagactctt ctatatattt
    10621 ttctcctaaa cttaaacctc tatcagatat ttgctttaga ttttgaaaac tatttaaaca
    10681 atctctttca aagtttttag atatttctaa agcatataat ttaaagattt tccttatctt
    10741 attataatta ttttttgtat catcaaatct gataatagct ccatttacat caaaagtaat
    10801 cattgagttt taacctcccc attagtttca tcaataaaat aaatttaata taaactttat
    10861 aatcataata ttaaacttat ttatactata attattaatt tgatgataaa agctattttc
    10921 cttctaaata aaaaatttgt ctaaagtttc taaaaaattt agacaaatct taggttatta
    10981 ctctttaaat tatttttaaa ttattccaaa tacgccaaat aatattggta taaatatagc
    11041 tggtaacaaa ttggctactt ttatattcgt taatttaagt acatttagac caagtcctat
    11101 aattaataaa cttccagctg ctgtcatttc tgtgactaca ggatcactta aaaatccatt
    11161 taaaaatgat gctcccacac atataatacc ttcatacaaa aatacagata ttgcagaaaa
    11221 cattacacct atacctaaag aagcagtaaa tataattgaa gctattccat ctataacaga
    11281 ttttacaaat aaagtatcat gacttcctgt aagaccactt tctaatgaac caactactgc
    11341 catggctcct atacagaata ataaactaga tgatataaaa ccctctgcta tagaatcttt
    11401 tttatttcct ttagaaaatt tactttgtaa aaaagcaccc aacttattta cccatttgtc
    11461 aatatctatt aattctccta tcagagcacc tacagctacg gatattattg tcactaatgt
    11521 gttttttcct tctagtgctc ctgatatacc aatgtacaat acacataaag caattccatt
    11581 cattatagta tcgcttactc tttgaggtaa tcctcctttt attataagac ctactataca
    11641 tccacctgca attgctagac tatttgctat tactccaaac attttttctc ccctcgatta
    11701 gctaattgaa atttatccta ttattaaaac ctaaacatct atactttgta ttggatattt
    11761 taaatattcc aaaaattctt caaaaccttt tctagatata tttacagttt tagtatttat
    11821 gttaggatga aaatttaagt aatcttcttt aattacatct ttatctatgt aaagctcaac
    11881 ttttctttct gtatcattta ttaatgaaaa aggatttaca cttccaggaa gaagtttaag
    11941 tacattgtat agtctttcag gtgaagcaaa cgataatcta gaacttccta tcttatcttt
    12001 aagtgagttt aaatcaactt gtttttctcc tttaactaca ataagataat acttatttcc
    12061 tttggcattt cttaaaaata aatttttaca ttgacacccc tttgttattc catctaaaat
    12121 gactaattgc tctgctgtat atacagcttc atgctctact acatcatatt caacatttaa
    12181 tttatccaaa atatcatata ttttagtttc attcatgcta gattcatttt catcttgttc
    12241 ttttttatta tttaaatcac tcatactttt ctccttaaac acttcatcat aatatcattg
    12301 tgaggtatta ttctactata gctattgctc ttactggagc tccatcagca tcattatact
    12361 taaatggtgc tgctataaat agaaattttt ctggaacatt ttctaaattt gtaagatttt
    12421 ctatgattat ttttcctttt tcaaataaaa tatgatgcac ttcaaatact gatgtgtcat
    12481 atctatccac tgatagcata tctattccta tgccttttat attagtatta gctatatagt
    12541 ttgcagcttc ttttgttaat gtaggataac ctacatagta ctgcttttta tcccaaaatt
    12601 ttgaccaacc tgatttaaat attataaaat cactattttt tatttcatct tcatattttt
    12661 ttagatattc taattctata ttctcatttc ccttttcaag ttccaataca taagctttcc
    12721 caacaaaatt ttctatatcc aacttatcta gggtctcacc tttagtatac atatgtcttg
    12781 gtgaatccat atgagttcca ttgtgagaaa atacactaat taatgtttct tgataaccat
    12841 tttcttcaat aatagctact ttcttgatat ctggtctatt aggctctgca tatacaggca
    12901 tgtcattatg tgtcacatga gttaaatcaa aaaccttcat ctttatatac cccctatgtt
    12961 tttattttct attatatacc taaattcatt ttatttctat cagattttat tccattatat
    13021 accatactac tatatatttt ttataaacct caagggattt accgaaaaat cttggaaaga
    13081 attatcagta aatataacat cttcacccaa aaaacgttta taccttcctt cttctttctt
    13141 taaagtaatt ctctgttttt gtgaatgatt attaaaaacc attatcttaa gaggaggttt
    13201 ttttctatta gtctgcactc tttttaacat gtattttatg tgcatatcag cttcaggaaa
    13261 tgaatatcca caaaatatta aaaggtcagt atctcttaaa gttttttctg tttcattcca
    13321 aacattactt aaaaatatgt ttgacatatt cttaaaatat gttggtggca ctataatagg
    13381 ttctgttaac tcaccacact ctaaacattt tgtctctgaa ctattttcaa taagcttcat
    13441 gacaccacct tcatgtggtg taagagttac actattacaa atggggcaat aaagccaatt
    13501 caatgaccca tgtatcttat ataatttgat taatggttct tttggttttc tccaatttaa
    13561 ctcctcttta aaatttgtaa actccacacc ataatcaagc ataattggca aatcattctt
    13621 atctcttaat ccagtaactg tattgtctat atgtatatca taatttgcac taataaaagt
    13681 agtatcaaga agcaaatcat tttccaaaag attttcaact aaaagtttat gatatttatt
    13741 atctgttcta ggtgtattat gaattgcttc agccattagc agtattaaat attgtcttaa
    13801 aaaacgtata ctatcacttc tgttatttag attttctagt ccaaaatttc taaatgcttc
    13861 ttttctttgt tctgctaagt ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc
    13921 attatctata ttatctttta ctatatcaat attgaacatc tgtttaaaga aaatatacaa
    13981 ttctctattc attttggtat tataatttgt gggatttagt tttttaaaat attcgctaaa
    14041 aagttcattt tgaataggta gattctctgc tgctgaagct cctgctccta aaaatattgc
    14101 tattttcata tagttcctcc taatataaat attactccta tttttatatc cattaaatat
    14161 ccatatcatt tattttatga tacaacaata aagctgtctt ttactagaaa aaatcctatt
    14221 acaagacagc cttatattat attaaatctt taataattcg taaatagaaa gagcatatat
    14281 ttgagtagct tttataatat cctctactat taagtattca ttagcttggt gttctgtttc
    14341 tggttttcct ggaaaaatag ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt
    14401 tgctcctcca gatgaaagtg gtgttccatc aagaccagtt tcttcttcat atacttttct
    14461 aagtgtctta acaagaagag tatcttctgg aacatagatt gaatccaaga aatcatattc
    14521 ttcataattt aaattgtatt tatctgtcat tttctttaac tcttttacaa aatcatcctt
    14581 tttgtatgtt actggaatcc ttacatctat tcctgcaaat tctttttcgt tatcaatagt
    14641 tactctacct atatttactg ttagtttacc tgatacatca tccttgcaat taggaattat
    14701 attgtttcca ttagcatctt caccaataac ttctgctaaa aacttaatta tattagaatc
    14761 tatacctata ttattaagag ctatacaaag tctagccact gcatttatac cagtatcact
    14821 tgctgctgaa tggacacttt ttccaattat acagatttta ttaccttcaa cagtatattc
    14881 aaaatttaat ttatcaagtt ctttttttaa tttatcagag tattttccaa tatagattgc
    14941 tttacctgga actgcattta aagcctttcc aactgacaac tctatatcac ttttgctatc
    15001 acaagtcaaa tgaacttgta aaagaccttt ctctgcattt gttattggga atctagaatc
    15061 tggagtaaat ccatagtttg gtatttcttc attattttct ttatatttat ttatacatct
    15121 ccaaagattt tcttcatcag ccccaaaaat gaatcttatc ctcttattaa agtctacatt
    15181 taaatccata agagctttca ctgcataaat tgctgatatt gtaggtcctt tatcatcttg
    15241 tgttcctctt ccatacaatt taccatcttc taaaacagct tcaaatggag ggtaattcca
    15301 gctttctaaa tcaccttctg gaaccacatc aacgtgacca agtataccta tcattttttc
    15361 accttgtcct atttctgcat atccataata tccatctttg tatactgtct taaatcctaa
    15421 agatttacat aagtctaata tacctcttaa agctttatct acattttcac caaatgggca
    15481 attttcagtt gcttcagata taacactagg tatttttaca ctttcttgta ttgaagaaat
    15541 catttcatct tgtagagaat ttactttttc tttaatttgc tctttcatat tatcaccctt
    15601 caattttcat cataatatac taataaatat tttgaccttt taaaaaaaaa atatgcttat
    15661 tttttcagtt tttttataaa ttcaatcgct tttaaagtcc ccatttttga atggtcaaat
    15721 gtaagtccac cttggaaata aacattataa ggaggtctta ttggagcatc tgcacttaat
    15781 tctatagaag aaccttgtat aaatgctcct gctgccatta tgacttcact ttcgtatcca
    15841 ggcatgtccc aaggaacagg ttttacataa gaatcgactg gtgcagcttc ttgtatacct
    15901 tcacaaaaac ttattacttc atcagcatta ttaagtctaa tacattgaat aatatcactt
    15961 cttaagtcat catattttgg taatacatca tatcctaatt tttcaaaagc tcttgagcaa
    16021 aagatagctc ccattacagc ctgagatact atataaggcg ccaagaaaaa accctgaagt
    16081 acatttctag ttgttccaaa agtaagacca cattcttttc ctattccagg agaagtcatt
    16141 ctataagata taagctcaat caaatctttt ctaccagcta tatatccacc tgtcaatgca
    16201 agcccaccac caggattttt tattaaagag ccagccataa catctgctcc aacatcagta
    16261 ggctctttgg tatctaaaaa ctcaccataa caattatcaa ccattactat agcttctggt
    16321 ttaacagact ttattacttc tatagcttct tttatatcac taattgaaag tgattttctc
    16381 caagaatatc cttttgacct ttgtatcata acgagtttag ttctatcatt tattttgctt
    16441 ttaattcctt ctaaatctaa gtttccatcc tctaaaaaat ctacatcatc atatgtaaca
    16501 ccatactctt tcaatgaacc tttttcttct cttatgccta taactccctc taaagtatca
    16561 tatggtctac cagtaacaga taaaatttca tctcctggtc taactatacc ttgtatacat
    16621 aaagtaagtg catgtgtacc atttacaatt attggtctta caagagcatc ttctgtatta
    16681 aatactttag aataaatttc ctctattttt tcacgaccaa tatcattata tccatatcct
    16741 gtagtccagt taaagtgcat atcacttagt ttagattctt gcatagcttt taaaacttta
    16801 tattgattat attctcttat ttcttttatt tcttcaaact tatcttttat ttcttccatt
    16861 atttcttgtg atagtttaaa agtatcatca tctattccat agtagtcttt taataattct
    16921 tttgtctcat taagcatttt atcacctgtc tctttattat tttatttaaa catgtaaagg
    16981 agactatttt actaaaacaa ataaattata gtttataagt tagtataagt ctcctctcaa
    17041 tcatcttaaa ttaaattaat tatctatctg ttgttattat tattgttgtt ttggttttga
    17101 tttgttagat taatatactt tccaggttgt atagttgata cagcatgttt ataaatcatg
    17161 ttttgttgtc tattatcccc ttctattaat attatataac tatcaaaccc ctttacaagc
    17221 cctttaactt gcactccatt aactaaatat atagtaacag gtatcctttc tttccttgca
    17281 ttatttaaaa acaaatcttg taaatttaaa actgtatttt tcattagatt tgtaccctcc
    17341 caagattaaa ttgaacttct aaaatattcc taccttatat tctatataag ttatgacaaa
    17401 ttttcaatat taagttgtat acttaaaaat ttatatatta ttttatagaa tctttaatat
    17461 ataagattat ttcatttttt aattcgtcta tattttcata ttggtctaaa tcaaaccatt
    17521 ttgctgtttt atatctttta aaccatgtta tctgacgttt tgcatacctt ctagaatctc
    17581 ttttaattat ttcaatagct tcttcgtatg tatattctcc atccagatac tttaatatct
    17641 ctttataacc tataccttgc attgatatca tatctttttt aaatcccata cttaaaagtt
    17701 tttttgcctc ttcaaccaat ccatttttaa tcataatatc aactctcata tttattcttt
    17761 gatacaaatg ttctctatct ctatttagaa ctattattat tggttgatat tcttcattaa
    17821 attttaaatc acttgaaaaa tcattcattt tcttaccact tagacaaact tcaagagctc
    17881 ttatgaccct tttagtattg tttttatgga ttctattagc agattcttca tcaagttctt
    17941 ttaacttatt atgcatgtaa tctattccct tttcagcaag ctgtttttga agttcttctc
    18001 ttaattcatt attggcatca gattgtgcaa aatccatatt atatattaaa gagtttaaat
    18061 aaagtcctgt gcctccagtt actaaaacac atttttcctt ttcatttatt tcatgtatat
    18121 atttttttgc tctttgttga aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat
    18181 ctataaggta atgcttcact ccttgcatct cttcctctgt aacttttgca cttccaatat
    18241 ccatatattc atatatttgc atagaatcag ctgaaattat ctctgcattc atatcttttg
    18301 ctaattttat tgacaaatct gtttttccaa cagcagttgg acctgtaagt attataagtg
    18361 gtattttttt cataacattc ctttccatat attttaaata tccaaatatt ttacataatt
    18421 cttttaaaca ttttttctat ttctgtctta gatatttcga ccattatagg tcttccatga
    18481 ggacatgtaa atggattctc acacttttct agctgttcca ataaactttt tatttcaata
    18541 tcgtatattt tatcatttgc ttttatagca gacctacatg ccatagatgc aaatctctct
    18601 ccttttaaat cataattact tgtaatttct tctatattat caattatttg aagtataaat
    18661 ttctcagttt caggtactcc aaatatagta ggaacacatc ttaccattat atgattattg
    18721 ccaaaaattt ctagctcaaa accaaatttc ataaataact ccaagttatt ttcaatttga
    18781 agcatatcta tatttgatac ttcaataacc actgggtcta aaagtatttg catatttata
    18841 tcttgtctat agaacttctc catatatctt tcatatagta ttctttcatg tgcagcatgt
    18901 tggtccaaca aatacataga atcgtctttg ctcaaaatta tataagtatt aaatacaact
    18961 ccaattacaa aatatccgta taaagaaaac ttgcgttttg aactacacaa gtattcttct
    19021 tcactgtcct taatactatc tcctaagtaa taatttcctt catttttaat tccatcaact
    19081 tgaaactctt cttgtatatt atcatttgca ctatcttcac ttagataatt aatgtttttt
    19141 acatcatctt ctacacttgc atttaggact tcacttactg attgaaattc atttattggt
    19201 ttttcacttg ataaagtaac aacttcaatg acttcatcct tacctttagt aatattacta
    19261 tttttaggag tagattctaa taaatcatta tttcttaatt tataatctgt acttttttct
    19321 ctactgttaa tagcgattct aggttgtgtt tttttatcac tataagtagc atattttcca
    19381 ataagatttg agtgtattaa tttaaccttt aaaaaatctc taagttctat atacacttct
    19441 tgttcttttt caaatttaat ttcaagttta ttggggtgta tatttacatc tatacatgat
    19501 gggtctactt ctatatttaa gaagcacact gcatgtttac ctattggaat tatagactta
    19561 taactttctg ttattgcatc tatgattatt ttacttttta caaatctttt atttatatag
    19621 atatgttgta agtttttatt tgaacggtat atattgttat tacctatata tccattcatt
    19681 ttaaagtgat tgcacttaaa ctctacatct attatatttt ccgttatttc ttttccatat
    19741 atacttctaa tagtgtttac aagttttcca tcccctggag tatttagcat ctgtttattg
    19801 ttatttgtat atttaaattg aatgtttgga ttacctattg caagtttatt tatcaaatca
    19861 cttatgttta tagtttctgc atgagtagat tttaaaaatt tctgtcttgc tggtgtatta
    19921 aagaaaatat cttttataat gatggtagtt ccattagtag aacctattgg ttctttcgat
    19981 attatttttc caccttcaac atatatttta gttcctatta tttcatcttt agttttagtc
    20041 gtcatttcta attttgatac agctgaaata gatgccaatg cttctcctct aaaaccaaga
    20101 gagtataaat catacaaatc atcaattttt ttaattttac tagtagcatg tctcaaaaat
    20161 gatttttcaa cttcacttga aggtattcct attccattat ctgtaatctt aattagactt
    20221 tttcctccat caattatatc tatggaaatt ttatttgcac ctgcatcaat ggagttttca
    20281 ataagttctt ttactacaga agaaggtctt tctacaactt cacctgctgc tatcttattt
    20341 atagtcaaat catctaggat gttaattata tttttcattt ttacacctct aagattttat
    20401 ttcttttgct tttctttgaa gattataaag agagtttata gcatctaatg gagtcatatt
    20461 taaaatatcc agatttaaaa tttcttctga tagtatatct ctatttacac tatcaaaaga
    20521 gatttgagtt aaggctactt cttttacaga atcttcttga ttaattttct ctttatcatc
    20581 attttgcttc attaaagcaa cttcatcatt taatgcttta aatttagaat ttaatttatc
    20641 ataatccttt tttatatgtt tgtagtcttt tactataagt tcatgttcta tttttagaga
    20701 ttcatattta ttcttaaaat tactttgatt taccttaact aattcttcgt ccaaattact
    20761 attaattata ttattgtttt ctttatctcc atttattgca acactgttgt aaacatgatt
    20821 tttttccaag tctttaagta tatattttgc tctttctatg acttcgtctg gtaatttagc
    20881 taatttagca acatatattc cataactctt gtctgctccc tgaggaatta tttttcttaa
    20941 aaatattatt ccttccccat cttctttgac tgctattgaa taatttttaa cctctttaaa
    21001 ctcttcttct aaatcagtaa gttcatgata atgtgttgca aataatgtct tacatctaat
    21061 atttttttga atatattcca ctattgacca tgctaaactt ataccatcat aggtactagt
    21121 tccacgacct atttcatcta atataactaa acttctctct gttgcatttt ttaatataag
    21181 ggatacttcg ttcatctcta ccataaaagt agattgacct tgagataaat cgtcactagc
    21241 tccaactctt gtaaatattc tatctaatat tggaatatca gcagactctg ctggtacaaa
    21301 tgagcctata tgtgccataa gagctattat agcagtttgc ctcatatatg ttgatttacc
    21361 tgacatatta ggaccagtta ttatgttaat aatgttttct ccacgattta aataagtatc
    21421 atttggaacg aaattttcct ctcctactat attttcgaca acaggatgtc taccatttct
    21481 tatatcaagt ttattatttt catttattgc tggttttacg tagttattta tatgtgcaac
    21541 tgttgcaagg gacacaaaaa catctatatt agctatagtc ttagccactt tttgtattct
    21601 atctatgttt ttatatattg tatctcttat ctctacaaat atctcgtatt ctaaagattt
    21661 aatcttttcc tctgcatgta gtatcttttc ttctatttcc tttaattctg gagttatata
    21721 tctttctgca ttactaagtg tttgctttct tatatatgtc tcatctagct ttgcctgttt
    21781 aaagttggct tttgttattt ctatataata cccaaaaact ttattaaatc ctatttttaa
    21841 tgatttaact cctgtttttt ctctttccct gttttctatt tcctttacaa gaaaggctcc
    21901 attcttagaa atctctctta gttccttaag ttcatcacta aaatctgatt taattatgtt
    21961 tccatctttt atggttatcg taggttcttc tagaatagct tcatctatta aattgtatat
    22021 atcatctaat ttatccattt ctgaaacata ctcttttagg atttttgcat ttgataaatt
    22081 tatagtatct tttaaattag gtaacttttc tatcgaattt tttaagtgaa ttaactcttt
    22141 tggtgttact ctctcaaatg ctatctttcc acatattctt tctatatcgt atatattttt
    22201 aagtatatca tttaggtctt ctcttaatat aaagtcatct tttatttctt ctattacatc
    22261 taatctattt tcaattttag atttatttat caaaggttct tctacatatt ttcttaaaag
    22321 tctacctccc attgctgtag aagttttgtc aagtacatgt aataatgaac cttttttctt
    22381 attccctctt atagtttgag taagttctaa attagttcta gtaaacatat ctagaaccat
    22441 atattctgat gaattgtata tatttatatt attaatgtta gatgttattt gtttttgagt
    22501 attgtatata taattcagaa gaattgataa gcttgacttt attagacctt tatcatcaaa
    22561 ctttagtttt tgtagataaa catctggaaa atattctttt aatatattta tatccaaata
    22621 gttatcacta aaactttcat ttatgtatat attacttaca gtagctatat ctctaagctt
    22681 ttctataaag tctaaatcat ttataattat ttcagttgga tggatttttg caatttcttc
    22741 aattacctta tcttcattta aacatgttgc attagtttct cctgtactta tatcaacata
    22801 agttagacct atattagtac catccttata caaagataat aaataattat tctttttatt
    22861 ttcaagtaaa tttccatcta aaactgtacc aggagtgatg acccttataa cctctcttct
    22921 aacaattcct tttgctgtag atgggtcttc catttgttca cctattgcta ctttatatcc
    22981 attttcaact aattttgata tatatgaatt ggctgagtgg aaaggaacac cacacatagg
    23041 agcacgttct tctaaaccac aagcttttcc tgtcaatgct atttcaagag cttttgaagc
    23101 tactaatgca tcttcaaaaa acatttcata aaaatctcca agtctaaaaa acagtataca
    23161 gtctttatat ctatttttta cttcaagata ctgtttcatc ataggagtta atttattcat
    23221 atctatttgc attatcaaac ctccttcttt tctaattaaa tattatatca tagtagatga
    23281 ttattattaa cacaatatat aaaattttga catataacta aaaacaatcc aaaatgtata
    23341 ctcacatttt ggattgctaa gtttaatgct tattaaataa aaatattttt ttttacaata
    23401 aagatattat ttctttttta agtttaatga tattttcaag gttatcttca tttaactttc
    23461 tactatctat aacaaattct ttctttacta ttgatggttt ttttgaaata acataaattc
    23521 tgtttgatag cattatagct tcgtcaatat catgagttat aagaagtatt gttgaattta
    23581 cttttttctt aacatctaaa agccattttt gcatggatgt tttagttaat gaatcaagtg
    23641 ccccaaaagg ctcatctaaa agcattatgt cattagaatt tataaaagtt ctcaagaagt
    23701 tagctctctg tctcattcct ccagaaagtt ctgaaggata tttatcttca tatccgctta
    23761 atccaaacac ctcaaaataa ggttttacca ttgacctagc tttactcttt ttttcttttt
    23821 ttaaaattag tggtagtgac acattatcta ttatagtttt atatggcaag agtaaatctt
    23881 tttgatacat ataactatac ttaccattta tattcacttt gccactatca taatctgtaa
    23941 gtttagttat aatattaaaa atagtacttt ttccacatcc acttggccct aatatagaaa
    24001 caaactcacc ttcataaaca tcaatactta tatcttttaa aacttcaata tctttataag
    24061 tttttgatac attttctata gatactttta cttttttatt gtggtaaaaa ttcatttgtg
    24121 taagcatcac ttgccttcat atccttatct attaatttat attcttttaa gaatgatgta
    24181 tagttgttcc atacactatc tttcatttct ccccatcgtg gtgcatcatc tttgtacttg
    24241 cttgctaaat attcttgact ctttaaagct agtttttcat ctacttcagg tgcatgtttt
    24301 actaaaattt ttgctgattc ttctggattt tttattgcat attcataacc ttttgttgta
    24361 gcttttaaga attttttagc aagctctgga ttatttttaa gcacagtttc attagaaatt
    24421 ataagtggtg tataataatc caatctctca tctaaatctt ttactggtat aaagtttaag
    24481 tcatatccaa ttaaatcagc tttaactgca tcccaacctt caaatatcca tgcaaaatct
    24541 acagttttca tagctgcaaa gaaatcatct tgaccagtat taactatctt taacttatta
    24601 aaatcagctc catttttctt cataactgct ttaaaaacag cttcttctga tggagagccc
    24661 caaccaccat aagtttttcc ttcaaaatct ttagctgttg ttatattctt ttcttttggt
    24721 gatgcaaacc cagaagtatt atgttgtata acagttgcta cagcttttat tggaagtgga
    24781 tcttcactag tctttgcata agtaacttgt tcttgatagc ttataccaaa gtcagctttt
    24841 cctgttgcta ctaaagtttc tgctccactt tcaggtggtt gtactatttc aacatccaaa
    24901 ccttcttctt tatagtaacc cttatcaagt gctacaaata atccagtatg atttgtattt
    24961 ggtgtccaat caagcaccat agttactttt ttagaagaag tatctttatc attgtttttg
    25021 tctttattgt tatttgaaga acatccagtt attgttgcca atgtaaaaac acttactaaa
    25081 cctaatattc ctaacttttt aaaattcata attgcctccc tcaaataccc ttaatctatt
    25141 aatctatgtc ttataacaac tttttttaca gcctgtacaa ttcctacaaa cattaaacta
    25201 aatataacta ctaatatagt agatgcaaat actttatcaa gtgcatatgc actttttgac
    25261 ctaagcatat aaacaccaag tcctttcgtt ccacctagcc attctgcaac agttgctgcc
    25321 attacagcat acgttgcaga tattttaagt ccagaaaaaa atttatccat tgccatagga
    25381 aatttaaggt gaatgaatgt attaacttta tttgagtcca tagtcttaaa taaatttaaa
    25441 taatctttat caatattctc aatgccatct acaaaactta ttaatatagg aaaaaaacaa
    25501 gtaagcatta ccatcaatac ttttggcatt gttccaaatc caaaccatat cataaagagt
    25561 ggtgctataa ttattgtagg tatcatctgt gtaaccaaca taataggata aagacatttt
    25621 tttattattg atacaaaatc catcaatatt ccaattacca atgatattat aattgcgact
    25681 ataaaaccaa gtattgcttc tcctatagta accacagcat gtatggataa attttgataa
    25741 tctttaaaaa acacattaat tatatctact ggtgtgggta gtatatactg aggaacttct
    25801 aaaactccta ccatagattg ccaaagaata agtataaaca aaaatgttgc tattggatat
    25861 atactatctt tgactttatc tatgatttct aagtttttca tctatagtca gacctccagc
    25921 tttttcatct actctagcta ctgttatgac tctttcacat ccaagattaa aagggatttt
    25981 atgtaccttt tttaataatt caaaaacttc atcaaaatca ccttcaacag tagtgctcat
    26041 tgctcctatt tcacatttaa gaccactttt ttcaatttca gcaatacaag catctactac
    26101 tttatacatc tcttcttcac ctttgtaagg tcttagtggc attactgcta tgtctgcgat
    26161 taccatttaa atcactcctt taaaaataat taatacctac taggtatatt ttgtttttta
    26221 ttaaaaaatt ttattactaa aaagatatta tatttaaata aaagaaaagt cttatccaaa
    26281 ataggataag acttattttt atcttaactg gtctccctac actggcatta tccaaacagg
    26341 ttcaaagggt cgagataaaa tctcctctca gcacattagc tccccgaaat atattatatc
    26401 ttttctactt gattatacta cacttatcat attttaacaa attttttatt tatattagat
    26461 attttatccg ttcaaaaata tctaatataa attgtacatt attacaccaa ataaattatc
    26521 ttaaaatggt aatttatcaa catcttccaa aactagatta actgaatttt ctagttttta
    26581 ttttgctatt tcaagcatat ttggatgtat atcaattcca tacaaatatt gtgctaaatt
    26641 tctgttttca ctcatctaat ttgcaatcgt tacaaattac agattagaaa tagcttttag
    26701 actctactta tctttatcta caaaaaatcc ttcaataaat tcattaataa tattcagtaa
    26761 tctaaaaata aacctctatc tagattttta agatacaagc cttttaggaa gtatcttttg
    26821 tgataatata aaaattactc atcattatca tcaaaatcct cagcaaccaa cattttagat
    26881 aagaatcaaa ctctataata taattatttt atccaattta tttttgagaa ataaaatatt
    26941 tttttaattt ttaaattttt taatatgcac cttgaaaaaa gaaacgtttt cctatattat
    27001 ggtaatagta gaataaaact aaataggcaa aactagagaa atttagtgac gcaaagctat
    27061 agggactaag acttatgaaa tgagttatgt cagccagttg ccaaagagat attttctttt
    27121 tgtttttatg aatgtttttt agggggatat atgatatatg tttatggata aaatggatag
    27181 atgtacacat atactaactg catatatatg cagttcacgc gattactgca attttataga
    27241 tacacaatta aatgatttta tactagaata tggagagaat gtggtagaat cttgtttgca
    27301 tcaagtgatg gtattggtaa gcagatataa ttaaagaaat tttattttct agaaaaccct
    27361 ttttagtttt atctacgttt tataattcat taaagaacta attaaaataa gcttcattat
    27421 atatttttca taaagtaaac agttgagttt atctcatata ctgtttaccc caattacata
    27481 attctaataa tatagattct aatgatttac cacgtttagt agacaaatat tcaacttttg
    27541 gaggaatagt cggataagaa atcctcttaa taagtccatc tctttcaaga tactttaatt
    27601 cttgtgacag tatcttatga cttatatgaa caacttcttt ttttaattga ctgtaacgta
    27661 ttggctcttg ttttaaaata tgccataaaa taattgccct ccatttactt cctatcacag
    27721 tcatggtaaa accaattgaa caattaaaat ctttttgtct ttcttcaata tttggaaatt
    27781 cttcaaaatt attatctata tccatctcaa tcctctcttt caaaatagta agtatctaac
    27841 taaaaagtgc attcttgttt agcgtactaa ttcgtaatat aatcttgatt gtagtataga
    27901 gccttacata atactacttt tttagatgta tatgtttaaa ctttaatata gacatatgtt
    27961 attttattaa ttttatttaa acagataata ataaataata atttatctca agttttctat
    28021 gtagataaac taatctaatg tttagaaagg attatataat atgaataaaa aatttcgtct
    28081 ttatcaagtt gactcattac aaaagaaaaa tttacaggta atccagctgg tgtaattaca
    28141 aatgctgatg gcttatctga atcacaaatg caaaaaatag caagagaatt aaataattca
    28201 gaaactgctt ttattttcaa taacttggct aaaaatcatc aagaaaatta tgatgtacat
    28261 atcagatttt ttacactaac taatgaggtt cctatatgtg gtcatgctac tattgctgcc
    28321 cattatgtta gagcagtaga aaaccaattc aataacaaaa cagtatttca aaaaactagt
    28381 gctggaatat taccagttga tattataaaa aaagataatg attatgaaat aattatgaca
    28441 caaggaaaaa ttgaagtatc tccaccgtta caacaaacct atcaaaaaag attacttaat
    28501 gcatttggta ttttaaagga agatattcgt acagattgtc ctattgccat atcatctaca
    28561 ggacattcaa aagttatgat tggaataaag gaattatcaa cattaaatag tttatctcca
    28621 aatttatatg aattatcgaa aataagtcaa gaaataaaat gtaatggata ttatattttt
    28681 acattacata ataaagaaac tctacaagtt catggaagaa tgtttgctcc tgcaattggt
    28741 atttcagaag accctgttac aggaaatgct aatggaccct taggtgcgta ccttgtacat
    28801 tataaagtaa tacctacacc caacacagag tttatatttg aagcagttca aggagaaaca
    28861 ataaaacgca aaggcaagat gttagtaaaa gtaattattt tagataataa accagttgaa
    28921 gtaaaaataa ttggaaaagc tgtaattgca ttctcaacaa acatagaaat ctaattatag
    28981 attccttcta agtaaatttt tgaacttttt aatactaatt taataacaaa ctaatttata
    29041 tacaatcaaa ttaatttttt tattat
//
LOCUS       DAELNY010000031        29059 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.031, whole genome shotgun sequence.
ACCESSION   DAELNY010000031 DAELNY010000000
VERSION     DAELNY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.031"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(110..1471)
                     /gene="rlmD"
                     /locus_tag="KPE55_001178"
     CDS             complement(110..1471)
                     /gene="rlmD"
                     /locus_tag="KPE55_001178"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0492272.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            complement(1498..2220)
                     /locus_tag="KPE55_001179"
     CDS             complement(1498..2220)
                     /locus_tag="KPE55_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0492273.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(2220..2549)
                     /locus_tag="KPE55_001180"
     CDS             complement(2220..2549)
                     /locus_tag="KPE55_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG0492274.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            2879..3805
                     /gene="fba"
                     /locus_tag="KPE55_001181"
     CDS             2879..3805
                     /gene="fba"
                     /locus_tag="KPE55_001181"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG0492275.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(3940..5571)
                     /locus_tag="KPE55_001182"
     CDS             complement(3940..5571)
                     /locus_tag="KPE55_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0492276.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(6054..7091)
                     /locus_tag="KPE55_001183"
     CDS             complement(6054..7091)
                     /locus_tag="KPE55_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0492277.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(7110..7895)
                     /locus_tag="KPE55_001184"
     CDS             complement(7110..7895)
                     /locus_tag="KPE55_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0492278.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(7928..9061)
                     /locus_tag="KPE55_001185"
     CDS             complement(7928..9061)
                     /locus_tag="KPE55_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0492279.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(9109..10236)
                     /gene="hadC"
                     /locus_tag="KPE55_001186"
     CDS             complement(9109..10236)
                     /gene="hadC"
                     /locus_tag="KPE55_001186"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG0492280.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(10236..11462)
                     /gene="hadB"
                     /locus_tag="KPE55_001187"
     CDS             complement(10236..11462)
                     /gene="hadB"
                     /locus_tag="KPE55_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG0492281.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(11477..12277)
                     /gene="hadI"
                     /locus_tag="KPE55_001188"
     CDS             complement(11477..12277)
                     /gene="hadI"
                     /locus_tag="KPE55_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG0492282.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(12316..13515)
                     /gene="hadA"
                     /locus_tag="KPE55_001189"
     CDS             complement(12316..13515)
                     /gene="hadA"
                     /locus_tag="KPE55_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG0492283.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            14192..15190
                     /locus_tag="KPE55_001190"
     CDS             14192..15190
                     /locus_tag="KPE55_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG0492284.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15552..16079
                     /locus_tag="KPE55_001191"
     CDS             15552..16079
                     /locus_tag="KPE55_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG0492285.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            16106..16702
                     /locus_tag="KPE55_001192"
     CDS             16106..16702
                     /locus_tag="KPE55_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG0492286.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16767..18350)
                     /gene="asnB"
                     /locus_tag="KPE55_001193"
     CDS             complement(16767..18350)
                     /gene="asnB"
                     /locus_tag="KPE55_001193"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0492287.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(18916..19752)
                     /locus_tag="KPE55_001194"
     CDS             complement(18916..19752)
                     /locus_tag="KPE55_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0492288.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(19776..21242)
                     /locus_tag="KPE55_001195"
     CDS             complement(19776..21242)
                     /locus_tag="KPE55_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0492289.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(21245..23107)
                     /locus_tag="KPE55_001196"
     CDS             complement(21245..23107)
                     /locus_tag="KPE55_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0492290.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(23354..24217)
                     /locus_tag="KPE55_001197"
     CDS             complement(23354..24217)
                     /locus_tag="KPE55_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0492291.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(24272..25204)
                     /locus_tag="KPE55_001198"
     CDS             complement(24272..25204)
                     /locus_tag="KPE55_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG0492292.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(25290..25841)
                     /locus_tag="KPE55_001199"
     CDS             complement(25290..25841)
                     /locus_tag="KPE55_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0492293.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(26070..26561)
                     /locus_tag="KPE55_001200"
     CDS             complement(26070..26561)
                     /locus_tag="KPE55_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492294.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(26589..27149)
                     /locus_tag="KPE55_001201"
     CDS             complement(26589..27149)
                     /locus_tag="KPE55_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0492295.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(27301..28458)
                     /locus_tag="KPE55_001202"
     CDS             complement(27301..28458)
                     /locus_tag="KPE55_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0492296.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(28495..>29059)
                     /locus_tag="KPE55_001203"
     CDS             complement(28495..>29059)
                     /locus_tag="KPE55_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492297.1"
                     /translation="NNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSEDIKKIDMESI
                     GIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNKCKEVVKEYEN
                     MQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYIGKITEYLDLS
                     DKQIESFKNRLNDLKEEINNSETNNIN"
BASE COUNT         9286 a         5296 c         3327 g        11150 t
ORIGIN      
        1 actacagaca catcaaaaca ccttttcaat tctttattcc taattttatt accctctaat
       61 ctaaatatta tatgtctatg aaactgtcta aagctcaaga tttctagcat tattttcgac
      121 gtagcaacac tacagtctca caatgtggcg tatttgggaa catatccata cacttaacca
      181 tcttaatctc ataaccatac cccttaaaat caaccaaatc aacaaccaaa gacttaggat
      241 tacaagaaat ataaacaatc tccttagcac caaatccaca aatatcccta atagcatcct
      301 tatgaatgcc tggtctagga gggtcaacaa taatcaaatc aggcttgtcc tttaaatcct
      361 taacaacctt agccacatcc ccagcaataa actcacaatt agtaagtcca ttcaacttag
      421 cattctcatt agcagcaaca acagcttcct caactatctc aataccatac actttcttag
      481 cagcaccagc cataacctgt ccaatagacc cagtaccact atacaagtca aatacaactt
      541 tatcattgta atctccaata aaatccctag caatactata aagaacctct gctcccttag
      601 tattggtttg gaaaaacgaa aatggtgaaa tcttaaactt aagacctaaa atttcttctt
      661 gtatataatc tcttccatac aatactctta actcatcaca ttggactgca tctgctaaac
      721 catcattaat agtatgaagt acacctacta actcagcttt taaatcaatc cctaaaagca
      781 tatctttaaa ctcatacata tcaaaatctt cttgagaaga agttactata ttaaccataa
      841 tctcattagt atgaatccct tttctaacta caagatgtct caaatatcct ttgtgattca
      901 ttgttctata ataaggaagc tttttttcat tgaaaaactc tacactagaa actaatatct
      961 ttgagaagtc ttcatcaacc aacatacact cctcaacagt ttgtatatct atatgctttc
     1021 cttttttatg aagacctaat gtcaaaggtc cattttttac ttcatctcca aaagtatatt
     1081 ccatcttatt tctataatat ttgttttctg gacttccttc tatccctaaa aactgaaatc
     1141 caaataaatc ttgctttaaa aataaatcca taacttgttt ttcttttatt tctaattgtt
     1201 tttcatatgg cacagacaat agtgtacatc ctccacactc tctaaagtgc ttgcaagttt
     1261 cttcagtttc aagtggtgag ttctctaata attctatcat cttaacttca gctttcttac
     1321 ttctaacttt tttaactcca gctctcacct tttgaccact tattccaccc ttcatatgga
     1381 taactctatc tccaacttgg cttaaagatg taccaccaaa ttccatttta tctacttcaa
     1441 attctataac atcttttttc ttcacagaca ttcctccaat ttactatagt atctttatta
     1501 tctttcttca atcaatttta cttcttcatc tgttaactct ctatactcac ctaactctaa
     1561 actttcatcc aattttaaat tgcccataga caatcttttt aagtatacta cttttttgcc
     1621 tacgctttca aacattcttt ttacttggtg aaacttacct tcatgtattg taagctctat
     1681 ttctgattca tcatcacttt ttaatatatt taactgtgat ggcatagttt tatatccgtc
     1741 atcaagcact acaccttcta aaaaagcttc tacatcttct tcagtaacta ctccatcaat
     1801 ttttgcataa taagtcttag gcacatgctt cttaggagat aaaactctat gagaaagctt
     1861 tccatcatta gttaaaacta ataatccctc tgtgtcttta tctaaccttc caacaggaaa
     1921 tggttcaaaa gctaagtatg ataaatcaat taaatctaat actgttgggt catatttatc
     1981 tgtagttgct gatatatatc catcaggttt attcatcatc aaatatatat attctctata
     2041 tataacttct tctccattaa ataatatctc atcattttca gtgtctactt gagttcctgg
     2101 atttgaaact tcacttccat ttactactac agacccttgt ttacaatact tctttatttc
     2161 acttctactt ccataaccta aatttgaaag aattttatct atacgttgct ttttagccat
     2221 taaaaatgct ctccattctc ataatatttt tctttttcaa atactgtaaa tgctaaatct
     2281 atactttcta catctaaatt atttcttata ctatcagtta ttattcttgc tactatatct
     2341 tgtacttctt gtcctctatc aaaccaagct acttcaacaa aaggatatac atctgctatt
     2401 ttaccatctc ttattgcaac tgactttata cattctattt caaagtagtc tcttggacat
     2461 tttacagctt caactaaatc atttatcatt ttttcactta ttttacatat atcattttca
     2521 ttaattcctc ttatttttat ctgtggcata gtttatctcc tttattttaa atttttagta
     2581 aattatcatc cacttaattc ctttcgataa cgtttgaagt ggtaacttct tggtcaaggc
     2641 aactattgtt atcagtatct ccaaactaat aaggtagttc ctacccttat agttagcatt
     2701 atatagctag ttttcctaaa ttgtttatat tctttattat tttatttaat cctattgtct
     2761 tctatttaac attatacttg taatttatag ttattacaat ttactatata ctttttttga
     2821 tttaacgtta taatataagt gatgggtaaa ttttaaaagt taataggagg agtttaaaat
     2881 ggcattaatt actacaaaag aaatgtttaa aaaagcttac gaaggtggat ttgctatagg
     2941 tgcatttaac ataagtgact tagaacaatt acaaggtgtt ttaaaagctg ctaaagctaa
     3001 aaattcttat gttatgatac aagcttctat gtcagctgtt aaatatgctg gacctcatac
     3061 attagttgaa atggttaagg ctgcttctga tgaaataggt gtagatgtag ctcttcactt
     3121 agaccatggt ccaaatatgg atgctattaa aacttgtatt gatgctggtt tctcttcagt
     3181 tatgatagat ggttctcatt ttgactttga agaaaatgtt agaataacta aagaagctgt
     3241 tgaatatgca cactctaaag gtgttgttgt tgaagcagaa cttggtgttc tagctggaac
     3301 agaagatgat gttacttctg atgttcacaa gtatactcaa ccagctgagg cagttgagtt
     3361 cgttgaaaga acaggtgttg attcattagc aatagctatt ggaacttctc atggtgcttt
     3421 caaattcaaa ggagaagcta aattaagatt cgatatacta gaagaaatcc aaagcaaatt
     3481 accaggtttc ccaatagttc ttcatggtgc atctgctgtt gaccaaaacg ctgttgctac
     3541 atgtaatgaa tttggtggaa atatagctgg tgctaaaggt gtacctgtag atatgttaag
     3601 aaaagcatct tctatggctg tttgtaaaat aaacatggat actgacttaa gactagctat
     3661 gactgctgct atcagaaaat tcttagctga aaatccaaaa gaatttgacc caagaaaata
     3721 cattggtgct ggtagagatg ctatacaagc tgtagttgaa agcaagatag atgatgtttt
     3781 aggttctgct aactctataa actaatataa cttttaaata tttataaaag aaaagagatg
     3841 acttaaagta attaaattta tttttaagcc atctctttta agttgtataa attatgtatc
     3901 ttataatatt ttaattatat tgtcttttta aactgacagc taaacgtgaa tatgtccact
     3961 ctttatcttt ctatggatgg tagaaggata tatacctata acttcagcag cctttgttat
     4021 tgaaccatac ttatttatag ccttacttat cattatttgg tccactattt tatatgcatc
     4081 attaagattc ataattccat ttaccactat tggtgataaa tcatctgttt cattatcaat
     4141 catatctaaa tttatcagca tgttaaattc atcttctcca attacatttt tagctgataa
     4201 tactacaaat ctttcaatga tgttttttaa ttctcttatg tttcctggcc aagaatattc
     4261 ttctaataac tcaataactt taggcgaaac ttctacttct ctattgtact tttcattgta
     4321 aagttttaag aaataattta ctaatggtac tatatcctct tttctttctc taagaggagg
     4381 tatttttact ggaataacac ttaatctata gtacaaatct tgtctaaatt taaggttatt
     4441 tctaaggtct tcatgagaaa tattagtagc acttatatat ctaacatcta tttttatagg
     4501 cttactacca cctattctag taatagtatt ttcttgtata accctaagta attttttttg
     4561 catctgcatt ggtaactcac ctatctcatc caagaaaata gttccaccat tcgcctcttc
     4621 aaaaagtccc tttttacctt ttttcttagc tccagtaaaa gaaccttctt catatccaaa
     4681 aaactctgac tctaataatt catttggtat agctccacag tttatagcta taaaaggttt
     4741 gtctctcctt gaactatact tatatattgt tcttgctatt atttcttttc ctacacctga
     4801 ttctccattt ataaatatag aagaatctga ggctgcagct ctcttaacaa ctttttttat
     4861 attgtccata atcttacttt gacttatata ctctgtgtct tctgatatat cactatattt
     4921 tatctgagat tcaagcttaa taacccttga aatatctctc cctgttagta ctcctcctgt
     4981 aagctttccc ttagaatccc atcttggaac tgccgtatat gcaataagct tcccatctgg
     5041 gtaaacgata tttctataaa tctttttctt cactttaagc atgtttaata ttattggttc
     5101 ttcaactaat ccttgtctca caaggtctgt tatatttcta ccaagtacct cagttctttt
     5161 aaaaggtaat atctcatcac tgacagtgtt catcttttca attaccccat ctttgttaaa
     5221 cacaacaaca acttcatcta tatgctctat tatatcttca attactttat cagatattag
     5281 agacttctca aataaataaa ctatattatt ttcattatta ttatctttca tattgtatat
     5341 atgtgcagtc atccttaact ctctgaaagc tattttcttt ttactatctg tcagtatttc
     5401 tgttttgtca aaatttgaat ctaattcatt tatatttcta ggctttttta tatctagaaa
     5461 actaaaaata ctatctatta gatgattaca gtatattatt tcgcctgatt ctttacataa
     5521 aatggctgga ttttctacat gttctataag ccctattaaa ttattatcca ttaatattcg
     5581 cacctttcat taagtaattt attgaactac atacaaaatt tattgtttgc ttacaataaa
     5641 taccatcagc acatataatc aactccttat aatattcgtc ttttcaagta gtttatttca
     5701 ttaaaacgta actaagatat aattttaatg attatagttt gttaaaaatt tcattttgct
     5761 tttaattgta ttttatcaca tatttagtaa ttatttgtct atttaataca atttaattaa
     5821 atttaagatt tatttaaaaa aacaaagata atccttacag aatataactc acaatttgta
     5881 agcaatatac atataaggat tatccagatt atcttgtatt ataaaatttt attcaaacag
     5941 actttttaat aactgacaaa tacacactag caataactta tcaaagggtt aataattatg
     6001 ttactaacta ataagactta ttcaatcagt aatatttata tagtttgtat ctattaagct
     6061 tttatagctt ctaataaatc atcaacgcta tctaattttt ctataagcat tggaactatt
     6121 tcatgtaagt ctccaactac tccatagtca gcgatttcta aaattggagc tgctgggttt
     6181 ttgtttatag caattatgaa atctgatgat tgcatacctg ctaaatgttg tattgctcct
     6241 gatataccac aagctatata taggtttggt ttaacagtag ttcctgtttg acctacttgg
     6301 tgagaatggt ctatccatcc agcatcaaca gccgcacgag aagaaccaac tactccacct
     6361 aatttgtcag ctaatttctt aagcatttca aatccttctg gtccacctaa tcctagacca
     6421 cctgatacta taacatttgc atctgtaaga gatactaaat ctttcttagt tttaactgtt
     6481 tctaaaacag tagttcttat atcatcttga gttattttgt agtctaatgc tataacttca
     6541 ccagttcttg tttcgtcttt ttcagcttta tccataacac caggtctaac tgtagacatt
     6601 tgaggtctat ggtttggaca aatgattgta gccattatgt ttccaccaaa tgctggacga
     6661 gtttgtttta ttttcttatc ttctgggtct atttctaact tagtacagtc agctgttaat
     6721 ccagttccaa ctcttgaagc tattctaggt gctaagtctc tacctatatg agttgctccg
     6781 aaaagcatta tttctggttt tatttcatct attgcatctt ttattacttt tgtatatgca
     6841 tcagttgtat atttttctaa aagagcatca tctgcaacat aaactttgtc agctccaaaa
     6901 gtgattaatt ctttagctaa tccatcaaca tcttttccta ataatattgc acaaagttct
     6961 gcatctactt ctttagctaa ttttcttcct tctcctaata attctataac tactggagct
     7021 atttttcctt ctctttgttc tgcaaatatc catacgtttt tataagaact taaatctttt
     7081 atatcattca tagtttgtac tcctccaact tatataatgt gcttttgttt tagaccgatt
     7141 attattttac tagctatttc atcagcttta ccttctaaga tttctccttt tcctcttggt
     7201 tcaggagtaa atgatctaaa tactttagtt ggtgatgctt ttaaaccaac ttcttcttta
     7261 tttacatcta aatcatcaat tgtccaaact tttacatcat gtttatatgc ttttactatt
     7321 ttatcaacag acatgtatct aggtgtattt aactctttta ctgctgttaa aagtacaggt
     7381 gtacttactt ttattaattc gtatccatct tctaattgtc tttgaactat tatatctttt
     7441 ccttctattt tgaaatcttg tacatatgta acttgaggaa tgtctaattt ctctgcaatt
     7501 tgtggtccaa cttgtgcagt atctccatct attgcttgtc ttcctgcaaa tattatgtca
     7561 tagtcaccta ctttagatat tcccgctgca atagtatttg atgtagccca agtatcagct
     7621 cctccaaatg ctctatcact taataatatt gcatcatcag cacccatagc taaacactct
     7681 cttagcataa agtctgcttg aggaggtccc attgttataa ctgttatagt tacatcatca
     7741 tgctcatctt taatttttaa agcttcctct aatgcatttg catcatctgg gtttaatata
     7801 cttggaactc catctcttat aagagtacct gtttctttat ttattcttac ttcgttagta
     7861 tctggaactt gttttacgca aactaatatt ttcaaagtct atacctccaa tttcattaga
     7921 ttatttatta ttttaatacg tttgatgaaa ttaccatttt ttgaacttct gatgttcctt
     7981 cgtatattga tacgattcta gcatctctgt acattctttc gatttcataa tcttttataa
     8041 atccatatcc accgtgaagt tgtaatgctt tataagcaac ttcatttgca atttctgatg
     8101 catagtattt agccatagca gattcttgag caactctcat tcctgcatct ctcttttgtg
     8161 ctgcatcata aactaatcct ctagctgcac aaacttttgt ttccatgtca gctatagtaa
     8221 attgagtatt ttggaattta gctataggct taccaaattg aactctttgt tttgtatatt
     8281 taacagcttc atctaaagca ccttgagcta ttcctagagc taaggcagca acacctattc
     8341 taccaacttc tagagtaccc attgctatct taaatccttg accttctttt ccaagtaagt
     8401 tttcttttgg aactttaacg ttttcaaata ttaagtcaga agtttcagtt cctcttatac
     8461 ccatcttatc ttcatgagct cctgttgaga aaccatccca tttactttct actataaacg
     8521 ctgatgttcc tctaagtcct ttacttctat ctgtaactgc tattactata gctacatcac
     8581 agaatggtcc attagttatg aaagtttttc taccatttaa tatatagtgg tctccaacta
     8641 attctgctgt tgtttgttgt ccacctgcat cagaaccagc tcctggctca gttaaaccaa
     8701 atgctcctat taattcacct gttgctatac cttttaagta tttttgtttt tgctcttcag
     8761 taccattgta aagtattggt aatgaagcaa gagaagaacc agctgtaaca aaagtagctg
     8821 ttgaagcaca tcttctagct atttcttcca ttactatagc gtaagaaaca tatcctgctc
     8881 ccccaccacc atattcttct gggatattac tgcttataaa ttttgcatca gctaattttt
     8941 tgattaattc cttaggcatt acacctgttt tatccatttc tgctggtaaa gtatctaatt
     9001 ctttactaac aaaatctctt actgcttttc ttaaaagttc ttgttctttg ttatataaca
     9061 tagcttcctc caattttcat attttatagt aaatttattt taatgaactt acgctaaact
     9121 catcatctca gcaaaagttt gaatacgagt tctggcttgt tcattattct gagtttgttg
     9181 gtcgatttca acatataaag taggtattcc actatcttct atatcttttc taactaaagg
     9241 ataatcgtat tcttctgggt cacagaattt catcatacag aagataacac catctatatc
     9301 tttcttttta acttcatcta ctataagtga cccacgtttt ttcttagggt cataagctaa
     9361 tgaacatcct tctatgtttg accattgtct tgctaatctt tctaacgcat catcacctgc
     9421 tggtacatct gttctgaatt gtcttgtttc ttgtgctaag tcgtcagcta caactgatat
     9481 attgttgtct tctaaaatgt ctaatatatc tttagaatca gctaatatac ctgttaataa
     9541 aactttcttt ccagaacaga cttcttctgg catagcattt aattttgcta ttaaatcttt
     9601 cactagctca gtatgttctt cttttctcat aaagaaccca ctcttaatta ctaagcttct
     9661 tattgatggt tttatagtat tagaatgttt ataagctact tcaacaaagt ctctcatagt
     9721 ttttctatgt tcattgtaaa cttctatact ttcatgaatt tttgcttctt ctatttcata
     9781 tccacaaatt tcttcaagtt ctctttttac gcctttgtac tcactgatta agtattttac
     9841 accagcttca agttttctat tttgtgggtg tactaatgat atatatttta tatgaggtac
     9901 tgctgatttc cagttttgtc ctaaacaaat tagtgtatca cacatacctg gtataataac
     9961 tccagataat tcatcataag caccttttaa tccatattct aaacatgatt gcattattga
    10021 acatgcaaat gcagggaaat attgtttagc taaatctaat tctgtttgcc ctccccatat
    10081 accaactgga agcattccag ctgcatgtat aatttcttct gggcaataaa ctgggaaaca
    10141 acctatagct tttttaccag tttcactttt atattttttt acagccgcat ttggattttc
    10201 aactacttct ttcattttag ataaaatagc ttccattata tctcacctct attaagtttt
    10261 tttctttctt ccattacttc aactaaacct tgaattcttg tttcaaattg agcattagtg
    10321 aaagctctag ggtctgcttg gtcaccatca aatccagcat atggtaatcc agtttcttca
    10381 gctgctcttc tttgcatttc atattgtatt aaactcataa gtttacagct tctgttcata
    10441 tgatagaatg ctccatcaca tttaccctct actaaagaat caactctata ttttgtcata
    10501 cggtctaggt ttacattgtt aaacatagta ctatatgcta ctgccattcc atctaaatca
    10561 ttaacttcat attgtaatgc ccaagcatgt gggtaaacac tacctgtcat gttaactcca
    10621 aatttagcta atgttttcat cttgtatcct atatatggcc aacaaggtat accttccatc
    10681 attattctgt atttttcttc ccctctgaaa gaagatttac cagttttcat gttgtcctct
    10741 aattcttcta taagtaactt aaatgcttct gtagtttctt ttttacctct agcacaaact
    10801 attacagcca tgtaagtaaa taagtcaaat ccattcattg gagaaggaga agaatctgct
    10861 ggtaaactca tagaatactt ccatagtctt ccgttttcag ctgatatttt cattacttct
    10921 tcaaacttct tagggtcaaa tttctttcct gatataattt ctagttgttt tatagcttct
    10981 tcaaattgag ctttaatata atctattctt gattgagtaa cttcgtcttc attattgaaa
    11041 gttgtatcaa tcattattaa aggtatatct aattctcttg aaatattttc ataccatttt
    11101 ataacttggt tacatatatt gttacagcaa agtaggaaat ctggagctgg catatccaaa
    11161 gcttcacatc caccattttc taaaagacca aaatttgttc ttgcatatgc acataggtca
    11221 atagaatatc ctttagattc agctatttca cataattcta aagaaccttt tttagctgct
    11281 actccagctg cttggttttc tggatataat acgtttaagt caaatacttc tgctaactct
    11341 tgaggaaata ctgatgttga ccaacctaca ggtcttcctt cttctttagc tttaaatgca
    11401 tttgcatatt gttcagctaa taaatcatta attactactc tagcttcttt tttttcagac
    11461 attttcatcc ctctttttat atatttttca cttctttttg tgattctttt gcttcatcaa
    11521 aagcatataa cgctgctcct aaagcaccag ttaattgagg tatatcaggt acaataattt
    11581 ctgtgttgat ttctcttgcc atagctctta caataccact atttctagca actccaccaa
    11641 ccataactac atttctttgt actcctattc tttttactag gctagaaact ctctttgcta
    11701 ctgaagtatg aatacctgcc actatatctt caatttttgc attttcagat aaatgtgata
    11761 taacctcaga ctctgcaaat actgtacatg tactgcttat tgatacttca ttttgagaat
    11821 tcatagatat acttccgagt tcagatacat caacctctat tatttttgcc attacatcta
    11881 aaaatcttcc tgtacctgca gcacatttgt cattcataag aaagtttaat agtcttccat
    11941 tattatctaa ttttaatacc tttgcatctt gaccacctat gtcaataatg gttcttgtct
    12001 ctggaattat gaaatttacc cctctagcat gacagcttaa ttcacttatt tgcttatcag
    12061 catctgaata gttcattctt ccatatcctg taactacaac ttttttaata tcttctcttg
    12121 caagacctgt cttgccatat aatttttcta aaactcttga tggtccagta gtcccagtac
    12181 cagaggatat tgtttcagaa gctacaatat cttccccatt ctttaagatt actccctttg
    12241 atgcagttga acctatatct aatcccattg tgtacatctt tagcattctc cttttaaaaa
    12301 atctagaatt actaattaat atcttacaac tttactatct ttaaagttat ttattttctc
    12361 ttcatcgtag cctaaagatt ttaatacttc tacagtatgt tgacctactt ttggtgctgg
    12421 tgtatattct ttaattcctt catttctaaa cataactgga gtattaacta agacacctgt
    12481 atttccgcta tcgtatgttt tcttaaataa gaagtcattt gcccaagctt gttcgtcatc
    12541 taataaatct tcacagcttt gaattttttc aaatggtaag tctgcttctt ctaataaagc
    12601 tgaccactcg tctaatgttt tttctaacat agcttctcca actatcttaa ctaaatcttc
    12661 aacatgatta accattgaat ctatgttatt atatctatca tcttctaata tatattctct
    12721 atttataacc ttacagaatt tgcctaacca cttgttgtat tgtattaaag ctaattgaat
    12781 ccatcttcca tctttacatt tatatgtagt cattaatggg ctgtttggat tttctcttga
    12841 taaaggcatt tcatttccgt attgtgctgt tgttatcatt gttcccattc catatatagc
    12901 tgtatggaaa agacttactg ttactctctc acctttacca gtttgagctt ttttatgtaa
    12961 tgctgctaaa cttcctgctg ctagtgctag acctgcatag tggtcaccaa atcctgctgc
    13021 tgtatttgct ggagatgttc ctttttccat aacagattgg ctaactcctc ctcttgcgaa
    13081 gtatgcagta tagtcaaatc ctggtttatc ttttaaaggt cctttttcac catatcctaa
    13141 tatttgagag aatattaatc ctggatactt atcttttatt tggtcataag ctatacccat
    13201 tttttctaat gcttgaactc taacattagt tacaaatatg tctgcttctg ataataattt
    13261 atgtaatatt tctactcctt cttttgattt tacattaata cttacaccct ttttatttcc
    13321 attttctaat tcaaacatag ggttttcatc atctgatgct ggagatttaa atgttccacc
    13381 cataactctt attccatcac cttctatagg ttcaatctta ataacctctg caccccagtc
    13441 acctaacatt tttgcacaac atggtgctgc gatgaaactt gaaagttcta ctactttaac
    13501 tccttctaaa agcataacgt tttcctcctt aatagtatca attataaaat ttcgattaaa
    13561 tcactcataa aatacttttt tctgatatta actgtctgat gtcaaagttc ctaatcttat
    13621 ctaaaataac ttaaaaatta ttaaaattat tttttattgt tttattgtct tacacctata
    13681 ttgttaagca tttatcgtgc caactttata tttaatcaaa atatcgcttg atttgaatgt
    13741 tttttaacaa tatgcaattg aataatcttt ttcatatcgt ggcatcttgc cactatccat
    13801 tgcattttgc tattctttct atgcttataa aagttttttt tatatatttt tttatgaaaa
    13861 agcttgacat ttcaaaaaaa gttttcattg ctaaactcaa aaacacatgt tatcattgta
    13921 attaatcagc aagttagtta actaatttca aacttagata attttttaat aatatttaat
    13981 ctaaaaaata acaatttctt tgataaattt tagagtaatt attacattaa ataaaaaatt
    14041 actaaatatc aaaataacac agggaaaaca atttcaaaac taaattttta accttaaaaa
    14101 ttttgtataa aaaatataac cttatatgct acatattatt tctaaatgaa aaattaccat
    14161 aataatttaa taattacagg agggtattga tatgaaaata ctagtatttg gagcacgcga
    14221 ttatgaagaa ccagtaataa aaaaatggtc tgaagaacat aaggatgttc aagtggatat
    14281 ttatcctgaa aacatgactg aagaaaatgt agttaaagct aaagggtatg atggtatatc
    14341 tatacaacaa actaactata tagataatcc ttatatttat gaaactttaa aagatgctgg
    14401 ggttaaagtt atagcttcaa gaactgcagg ggttgacatg atacattttg atttagttaa
    14461 tgaaaatgga cttatcgtta caaacgttcc ttcttattca cctaatgcaa tagctgaatt
    14521 agctgttact caagctatga accttttaag aaagactcct ctagtaaaga aaaaagtctg
    14581 tgaaggtgat taccgttgga tagctgaact tcttggaaca gaagttagat ctattacagt
    14641 tggtgttata ggtacaggaa aaataggtgc tacttctgca aaattattca aaggcctagg
    14701 agctaatgta attgcatttg accaatatcc aaatagtgat ttaaatgata tattaactta
    14761 caaagattct ttagaagacc ttctaaaaga agctgacctt ataacattac atactccttt
    14821 acttgaagga acaaaacata tgataaataa agatactcta gctataatga aggatggagc
    14881 ttacatagta aatactggcc gtggtggttt aattaataca ggggatttaa tagaagcact
    14941 agagtcagga aaaattagag ctgctgccct tgatacattt gaaactgaag gattgttctt
    15001 aaacaaaaaa atgaatcctg gagaattaac tgacccagaa ataaataaac ttctttctat
    15061 ggaacaagtt atattcactc atcaccttgg tttcttcact agtacagcga ttgaaaatat
    15121 agtttattct agtttaagta gtgctgtaga agttataaaa acaggaactg ctactaatag
    15181 agtaaattag tattttataa atatattttt tcattaatta gctattatct aacaaaatag
    15241 cctttaattt cccaaatata agagagtcag ctcagtatat attgagtaga ctctcttata
    15301 ttatttttta tattaaactt gtactcttct tattcatacc aaattaaatt tatattaact
    15361 tattctaact actcctactc tatacatttt acctattttt acttttctaa ttatatacat
    15421 ttgaatgggt acaatctgta ttttacaaaa aaaacaccta acaaactatg aaaattgtat
    15481 gattaatatt aatatattta taagaaaaca taacaattat gttagctctg gatttttagg
    15541 gagggagtat attggaaaaa atgaataaag ttacttctaa aaaaactacc tatattgtta
    15601 cttcagcatt atttgcatcc ataatatgcc tgacaatagc atatatatta catattccag
    15661 ttggtggaaa caatggatat gttcatatag gagatgcttt tatttatcta gctgcaacta
    15721 ttttgccaac aaactatgct atagctgctt ctgcaattgg tgctggtcta gctgatttat
    15781 ctacaggtgc tgctatctgg gtaataccaa ctataatcat aaaaccaata cttgtattat
    15841 ttttcacttc taaaagtgac aagataataa ataagagaaa tatagttgct tcggttgtag
    15901 caggtatagt aggattagtt ttatacatgt ttgctgaagg tataattatt ggaagtttta
    15961 caagtgcatt tgtaatgagc ttacttggct tattacagcc aataggaagt tttattgttt
    16021 ttataatctt aggtatggca cttgataaac ttgattttaa aaaacgttat tttaattaag
    16081 aatttaaatt tatggaggga aaattatgaa tatcttatat acaattgaaa atagtatcta
    16141 tgtaaatata actaatacat gtccatgtag ttgtgttttt tgtataagaa acgaaaaaga
    16201 tgaagttgca aatagtggta gtttatggtt ggagcatgag ccaagtgtag atgaagtaaa
    16261 agaagccttc aataaataca atttagacga ctatgatgaa atcgtatttt gtggatatgg
    16321 cgaaccatta atgagaataa atgaattgat agagatagca aagtttataa aagaaaaaag
    16381 tagcataaag ataagaataa atacaaatgg tctaagtgat ttaatacata acaaaaaaac
    16441 agctattctg ttaaaaaatg ttatagatgc agtatcaata agtcttaatg ccccaaataa
    16501 agaagcttat aatagagtta ctcaaccaaa gtttggagaa aaatcatttg attatatgtt
    16561 agattttgca agagattgca aaaaatatat aaaagaagta gctttttcag tagtagatga
    16621 aatatctcca gaagaaatag aagaatctaa acaactggca aaaaaacttg atataccatt
    16681 aagagttaga cataagaatt agcaagtaaa attataaaac attaagaatc acctgaatgg
    16741 tatttcaggt gattcttcct cttgtattaa cattttacaa aatagatttc ataccacact
    16801 aggaatgtgt aaatcatcca aatctttctc caattatcag agtttccact aatgtagtca
    16861 ttaaaaatct cattaatctc atctatgtta aagaatttat ctgctatctc actattgaat
    16921 ttatctcgga tatccttatt atatcggtca tcagccatcc atatacgaat tggcacaata
    16981 aatcctaatt tatttctaaa agcaatctct tctggtaatg tttttgatgc agctgtacga
    17041 aaggcaattt tattctcttt tcctttcact ttatattctg atggtattct ggatgcaata
    17101 tcaaatattc ttgtatctgt aagaggcatt ctgacttcca atcctactgc agtactcatt
    17161 ttatctatat taaaataaat atctccttcc aaccatattt gaatatctac atttgacatg
    17221 ttagtaagtg tatctagttc ttctgtttct tcataaattc cttttaccac ttcaatagga
    17281 agcacatcag gattgtattt cttaagaatt tttcttttct caccctcatt cataatgttt
    17341 gtatttccaa tataaaatgt cttcaaattc tctccataac actctgcctt atggtatata
    17401 ttatatccac caaaaaactc atctgctcct tctccagagt aacagatatt tgtgttttca
    17461 gctgctgcct tgcatccaat agcaaaaacg atagcagatg catctccagt tggttgctcc
    17521 atatggtaca ttacataagg tactgacgca aaatactctt caggtgtaat tatatatctc
    17581 gaatttttac acccaagaat attagctgtc tgttgtgcta attttgattc atcaaaacgc
    17641 tcttcctcat aaccacatga acctgttttt tctatatctg acattgccaa tatataagat
    17701 gaatcaacac ctcctgatag gaataactct gcagtttcat tctctgattt tacctctggc
    17761 ataattttct gaagtgttgt atgaatttca tccgtccatt cctcaataga cttgctgtgg
    17821 tcaggatgaa attcaggttt ccaataacga ccaatacata ctgttttatt ctgatagaca
    17881 aggtaatgtc ctggcataag cttctttact ccacggaaga atgtattttc acctgctaca
    17941 tatgtgaaag acatataaat ctgtaacatg tcttcattta actctttcac aaatcctggt
    18001 tgctccataa tcttacgaat agatgaacca tagagaagct tcccattttc agtctcataa
    18061 taatagaaag gttttacccc aaactggtct cttaggcaga agagtttctt ttccacagta
    18121 tcccataatg caaaagcaaa cattccatgc atgtgatttg ccatatcata tccccatgtc
    18181 ttatatgctt ccactaaaat tgctcgttca cgctcatctc tacataatga agaatcgatt
    18241 ccaagttctt cacaaagaac tttccagttt cgaatatgcc cattgtattc tagaattttt
    18301 atcatatatg tgtcttttcg tgttaaatac ttttcttcta aatttatcat atgtgtatcc
    18361 tttcgtatta aaacatttaa cttctaaatt tattaaatgt ttatatatat ttattcactt
    18421 ataatcactg aaaaatcgac ttttttcaaa gtaaattctt tttgaattag tactaatctg
    18481 ctctttatta atggcataaa tatgattatc aagtattatc agtgttataa cattgaattt
    18541 tttattcttc ataaatattg ccccctttat attaccaact aaatataata tatcttatgt
    18601 tacttacata ttctttaaga aattctgaag attttataaa gttttttgta ttataattaa
    18661 ttaaaaataa aattctagtt ataaaacata ttttccatta aacaaaaaaa tagttaaaat
    18721 aaattcataa ccacataaac ttattttaac tatttgtata ttttaattat aaatattaac
    18781 ttaaattttt aacaggatac actcataaaa taaacataat gaacaacaaa ataatatgtc
    18841 tcagtttctt tatatacttt accttctttc aattttctac catgtagacg tataagataa
    18901 tattagtaat tcactctatt gcttaactcg tgcgatatgt acaaccaaga aagctatttc
    18961 ctcatttgtt aacttttttc catatttctt ttctaaaaaa tctctaactt ttaatgcaat
    19021 taacatttct tttggatttg accttttcat ttgattaaat aataaatcat caccactgct
    19081 taacatacta tctgaaaaaa atctttccac aaaaaatttt atatgtgtta taaatctcat
    19141 ataatgcata ctctttttat ctaaggttct attcaaagaa aaaacaaaaa tattaattat
    19201 ttctccaatt actttggcat acttaatcgt atcatatgaa tttccatctg aagccatagc
    19261 atttgcaaaa tggaaagcaa tgttagctgc ttcttcctct ggtaattgaa tgcccaatct
    19321 ctcctctacc atataaattg caaccattcc aaccttaaac tcatttggat aataattttt
    19381 tatttcccaa aatactcgat ttgtaatttt tattccttta cgcatccttt caattgcaaa
    19441 gttaaaatgg tctgctaata ttaagtataa gtttttatta aaagatgagt tcaataactt
    19501 ttctgcctct actattattt cctgtgttat ctttaaaatg tcgacaggaa tatcatccat
    19561 taattctaaa aattgttttg attttgtatt ttcaacaggg acaaacattt gattatcatt
    19621 ggaaccacaa atatattctc ctgttttttt accatatcca attcctttcc ctagcaaaat
    19681 aaattcatta tactttgcat catgaactaa aacaacgctc gagttcaaaa ctttctttat
    19741 tatgtagttc attttatttg ctcaccttct attggtcatt taaaatctat actattattt
    19801 tcaattacct ttttatacca ataaaaacta tctttcttat atcgattata tgtccccttt
    19861 ccatagtcat cagcatccac ataaatatat ccataacgtt ttgacatttg tttcgttgat
    19921 tcactgacca aatcaataca accccatgaa gtatatcccc aaagttcaac gccatcttcg
    19981 ataatcgcat ctaacataca tttaaagtgt tcttttaaat aattaattct atattggtca
    20041 tgaacttttt tatcttcagt taaaatatct ttagctccca atccattttc aactataaat
    20101 aaaggctttc tgtatctatc atataattca atcaaagata tacgtagacc tattgaatca
    20161 atttgccaac cccattcact catttctaaa taaggatttt ttacagctaa taacgtattc
    20221 ccaggtgtaa tatctaatcc agttgtatca gctgcctcac aagaagacat atagtaagag
    20281 aatgaaataa aatctactgg atactttttc ataatttcat catcatgttc ttcttttact
    20341 atttgaatgt tattattttt aaaatacgac agtaaatata ctggatactc accaaaaacc
    20401 tgcgtatcac tatatgcata aatacttctc attttttgtt gagttgccaa aacatcttct
    20461 ggtctacatg tatatggata atacgttaat tttgttaaca tacaccctac ttttgaattt
    20521 ggaataatct catggcaaat ttttgttgca atggcactag ctacaaattg atgatgcata
    20581 gcttgaaaaa taacttcctc aaagttttta tccttaaaac ggtctcgaac taaaccacca
    20641 gtagtgtacg gatgacgaat catagaatca acttcattaa atgtaagcca atatttcact
    20701 ttgtttttat atctttcaca gatagtttct acatacctaa caaacatatt gataacttgt
    20761 cgtgaatacc atccatcata gtttaaaacg atatttaatg gtggttcata atgtgacata
    20821 gtaacaagtg gttcaattcc ataacttatc aactcatcaa atacatcatc ataaaactgt
    20881 aaaccttctt catttggctc tttatcatct ccatttggaa aaatacgaga ccaagcaata
    20941 ctcattcgat atgtcttaaa tcccatttct gccattagag caatatcttc tttatagtga
    21001 tgataaaaat cactaccatg acgttttgga taatttactt catcttcagg atgtgctaat
    21061 ccttcttcta tatcttttgt tgtgatttca ttattagatt tataatcttt cacatctgca
    21121 tcaaagtgtg cctttgctac atctgataca gaccaccctt tcccacctaa attccaacca
    21181 ccttcaaatt gattagcagc tgtagctcct ccccaaagaa atccttctgg aaattttctc
    21241 atatttatat tcctcccttt acatcaaaca agatacttcc tttttttaca ttgccataag
    21301 actggtttaa cactttataa gcataatctg ctccattatt gtttacaatc ataacagttg
    21361 aatctaaatt agcatgtgag attttgtcta aatcaaattg aattaataaa tctccttctt
    21421 ttacattatc gcctgtctta acaaatgttt taaatccatc tccattcata cttacagtat
    21481 ttattccaat atgaatcaaa atctcagccc cattttttgt tcttaaacca attgcatgtt
    21541 ttgtttcaaa taacatcaca acttctgaat cacaaggtgc gtataaattt ccattggttg
    21601 gtataattgc aactccatct cccaaagtct tacttgcaaa tagttcatca ttaaccttac
    21661 ttaaatctat tacttttcct tctactggag ttactaagat atttacttct ttttcttctt
    21721 ctagctcttc ttcaacaaca tctccaattg atgactctac tgtatttgta tcttccttcc
    21781 aaataatcag tgtaataata aacgagccta atattgcaat aacaaaacca atactcgcaa
    21841 aaatcaaatt gtttccattg tttgggtcaa taaacatact catactggca agccctggtc
    21901 caacaattac aaatcctttt actgcaaata atcctaaaaa ggtaccagaa attgcacttg
    21961 agaatacaac acctaacatt gctcttttat gttgtaaggt aataccatat aaagctggct
    22021 ctgtaattcc catcaaggca gaaattcctg cagataatgc tgtttgtttt aatgtttcat
    22081 cttttgttcg caaggctact gcaaaacaag caccactttc agaaatattg tgggctaatg
    22141 aagatgtcat atataaagct tcataaccca actttccaat tgtattaatt gaataaggta
    22201 ttagtgcttt gtgcattcct gttgcaacca taaatggaag aaaagcagca agtaatccta
    22261 caactaagaa tcccattttg ttatataaga ataaaataat ggttgtaaaa attgtaccaa
    22321 cgttatatcc taaaggacct aaaatagtta gagtaattgg aacagtaatt gccaaagaca
    22381 tcataggaac aaagaaagtt cgaattggtt ttggtgaata cttgttaaaa attttctcca
    22441 ttgttgccaa gaaagacaca cataagatag ccgggaaaac ctgtgcatta taggcaatgt
    22501 ttggaattgt aaatccaaat agttcaacac cttcacttaa agcagttgtt gttgctggaa
    22561 gcaataagta tcctacagcg gcaatagcta ctaaacgatt acatttcaat acattggcag
    22621 tagttacagc taccataaga ggtaggaaat agaatgttgc atctgaaata gcaactaata
    22681 atgtatatat agtagaatct gattttaaaa tattaaacat tgctaataac atcaacagtg
    22741 attttaaaat accagctcct gcaatagcaa aaatcagtgg ttgaaaaata ttaataataa
    22801 attctataaa tcttgttcct aagtttattt tttcttcagt ttttacagta ctatcatttg
    22861 tatctaaagg gcaaactttt aaaatttcat catacacatc aacaacacta tttccaatga
    22921 caacttgaaa ttgtgcccca ttaactatac tcataacacc ttgtactttt tttaaagcat
    22981 cttgattgac tttacttcta tcaatcaact taaagcgtag tctagttgaa cagtgttcta
    23041 aagctaaaat attttttgaa ccaccaactt gttcaataat gtcaatggca gtttttttat
    23101 aattcatctt gtaatctcct tctccataat tttttatgaa tggccattca agatgcaaaa
    23161 aaggcagaac ctatataaac accttgaaaa atcaaagcaa tatattcgat cctgcctgca
    23221 ttaccagtaa catgtcttct atttatacta ttttgtatta ttatattaac atttgcctaa
    23281 aagaattgtc aatacttttt taaattttct gatactaact aatagcacct ttataattca
    23341 ttttttatgt caattaaagt aacagtagcc acccaccaat tagtatcaaa attgaacctg
    23401 gtattttttc ttttatattt tctttgaata taaaatgagc aagtattaaa gttattatca
    23461 tagatacttg agaaaatggc tctgctatac taagttctag attatttaaa gcaattaata
    23521 atgctaaata agaatatcca ttaatagcac cacttaaaat tgaaagcccc tttttattag
    23581 taaaaatctc acaaattatt ttttgtttct ttttaaatgc taaaaagata aataaattta
    23641 aacctacgaa aaaatacagt atagttgaat ataatatagg agatacttgt acaaaaaaag
    23701 ccttatccaa tactcttcct actgactgca aaaatatgta taaaaacatt aatctacatg
    23761 gtaaattgtt aaaaattaca gagacattct ttaaaaaaga acttactttt tttaatataa
    23821 acactccacc aatcataatt atgattcctg caacttttgc taaacttaaa ccttcaccta
    23881 aaaatataac tgataaaagc agtaaaaaca atgaatttaa actatttatt ggagtaacaa
    23941 gtgaaacttc cccagttgct aaagacgtta catacgctac tgaaccaatt gaatagacta
    24001 aactacttat ataacatggt attaaaaacg accaatcact aactctatcc attatgctaa
    24061 atggaagtaa acatactccc cctataaaga aaaatagaaa tgatgaagct atatttttat
    24121 aaatatctcc ttcttcatca cctaataatc ttacaactac tctttcaaaa gctaatagca
    24181 ctactctaat taataatgca aaataagata atatcatttt tgacctcgct taaaaattaa
    24241 tccgacttat tgttatatta actgtataag actaccatat aggtaagtct tttggatttt
    24301 ttaatatttt atgtttaaat acaggtacat taagtttttc acctagttta cggcattgct
    24361 ctttataggt accttttaat ttatcatgaa aaactataca atcaattcct ccttcaatgc
    24421 tatttataca cttttctagt ttctcaaatc cagattcatc atcatacatt ccacttacaa
    24481 tccattgatt tggcaataca tgtccaattg atatgttata ttctttattt tttataatat
    24541 ctgagaaaga tttctcaaaa tgtacccctc caacacataa caaagtcttc aaaggttcat
    24601 cacaatcaaa tactctaatt aatgattttg ccaatacctg tattgctata ctattattaa
    24661 aactatcact actacttcct atctcaatat ccaacatagg tacagaatat tctgtaatta
    24721 atttagattc ttcaccatat gttgttccag accagtgagt tgcttctgtt aatgtagtaa
    24781 attcatcaag tagattttca ttttttatat cctcaatagc attgattaga tttttaaagt
    24841 atcttggatt tgaatttcca aattctcctg ttggaacatc tccaattgta tgagctgtta
    24901 ggatatgttc tggagcattt tctccctcat gccaatttac aactcctgca aaatcataat
    24961 ctctaaaatg tctatttaaa attggaagat aatatttata attatgactt attacatctt
    25021 ttgtttttac aaaatgaaat aagttattgt ttttatcctt aaactctaat acaaaattat
    25081 catcaataac aatatcagtt tgatttaaat ccattatttc ttcacaacat tttatcacat
    25141 tactggctac tgggtctttc tttaaatcac tgcaaaagaa aaaaactgct tttttattat
    25201 tcatccataa atcacactcc tataacttaa atataatata tcttcatacc taaaatcatc
    25261 taaatatcac tctaattcca ttaaattatt tatatataat atactaccat actcatttta
    25321 gctatacctt tgcctgagtt tctatatata tgtgatacat cagctttaaa tcttatagaa
    25381 tttccactag atacaatgta atcttctcca tttattttaa tggttacttc gccactaaaa
    25441 acagttataa actcttgtgt tcctggaagt tcagcatttg aatctaaatt tccaccttct
    25501 tctatttcaa tcataaatat ctcaaatctt cttttggagt caaatggaaa tattggataa
    25561 cttatatact ttccattatc ttcaataaaa tggtctatat cacttttttg aatcaattct
    25621 atgtcacttt cctgcctctc catcaatgat gtaaatgaaa tttttagccc attagttatt
    25681 ttcttcaaag tagatatagt aggatttacc tcacactttt caatttgaga tagcatactc
    25741 ttacttacac cactaagttg tgcaatcttg tctaaactaa gatgtttttc tttccttatc
    25801 ttttttaaat tttcagcaat tatttcattt aaactttgca taaatacccc ctaatacata
    25861 ctaatataag ataaattatt ttgattatta aataaactta gtaatttaat agaaactttt
    25921 tatccattat aatgtacatt atgtttaata tacattactt tttttagaat gtaaatagtt
    25981 ttctgaaatt ttgtgaaaat gaataaataa aaaactagta acctatattt ttacaaggtt
    26041 actagctttt attataataa tatttcattt taactataaa acttagatgc acctataagc
    26101 tgtctcttag aaacaatctt agctaggttt gctaatgaag aaatttttat atctttaaaa
    26161 gaaactaaaa atacaaactt aaaatctgta aattcttgat ttatattgta tatatcaaaa
    26221 taacatctca aattatcaaa taagtaatta tcctgtaagt ctgcttctat ctcatcagta
    26281 gcatctttta aatacgctct tacatcttca aataaaacac tgaaataaga ctcatctatt
    26341 tcttcaccta actcttccaa tatttcttct atatctaaat catccatatc aaaagaattt
    26401 cctgtcaact ggcaatcaaa taccaatata ttttcttgat ttatagtatt tgatatatct
    26461 tgtacatatt cgaagtctgg acgtaattct aaaatgtctt ttatttcact gctctttaca
    26521 tggatttttc cttttctatt ttcaattttt aaatctatca tatgctcccc ctttcaatct
    26581 acaaatcttt aaataccttg ttttataatc ttttcaatat ctatccaatt actaactcta
    26641 actatatttt gatgtttaac atctttatta tagttagtat ccagaagtat tactttaaca
    26701 ccatcatcag ctagttgcac tgagtttaaa ggattatctt ctataaatat gtcacaactc
    26761 agttctcttg ccttttcaac tttataatca cttcctaata agtaaacatc tatatctgaa
    26821 aatccttgtc tcgatagcca atttttagtt acatcagtta aacactcatg tctagccgtt
    26881 acaaaagata aattatgtcc tttagataat gtgctaatta cctctgtagc accctctaca
    26941 atctttgctt catcatatga gtgtatatac ttattattaa actccgcata cacatctctt
    27001 attcccgaac catacaattc ttcccaatta tgtgttatac actctgcttc agttatattt
    27061 ttattaaaga tatcatttag gtatggaaga aaatgataag ggcttgttat tgttccatct
    27121 atatcaatac atatattcaa tttgttcatt tttacctcca tgtaacattt attgatttga
    27181 ttaatcaaat attatagcac agctttaatt ataaatcaaa actctggata tgactatacc
    27241 tatataatac caaaaagctt gcatatttaa gcaagctttt cgtaaatttt acatttttaa
    27301 ctaatatata agtttttcta aggttggttc cactccaaat tgtgatttca gttctttata
    27361 ttcttttaat gctttgtcat tattcgtttt agtttttatc gctcttatca ttaaattttt
    27421 tggtgtatca agtggagata tgtattctac tacagaagta tcatatccat tgccctccaa
    27481 taacaatgtt cttaaaccat cagttattaa atctgcaaat ctagctttaa atacaccatg
    27541 ttttaatatt ggttccatag cttcatatct atattgccct aataactcct tatgacaaca
    27601 tggtactact actatagctt cagattttgc tctaattcct agacctattg ccatatctgt
    27661 agctgtatca caagcatgta gacttattac aaggtcaaca tccctattag gtgtataatt
    27721 agtcaaatcc tcttttataa atgacatatt tttataaccc aagtttttag ccatattttt
    27781 tgatgcttct ataactacat ctgaataatc tattcctata aaataacagt ttttcttcaa
    27841 tacttctttt atataaaaat ttaagacaaa gcttaagtaa gattttccac aagcacaatc
    27901 caagatagtt atacagtctt tatcctttat ttcttttaaa atactatcta tcaattcaac
    27961 aaaatggtct atctgattgt attttcttat tttatcattt tttatcttac catcttttgt
    28021 aagtactcca atttctttta aaagtgcatt tgcttgtcct acttttacat aataatctct
    28081 gttttttatt tgagatgccg taaattcatc tattttaggt acttcttcct tattatctaa
    28141 atattttaca tttaccttct tattgtctcc ttcaattaca acaatagttc ctcgctcaac
    28201 atatttaaat actaatccat cataattttc tgcctcttta gaaatattct taagcaactc
    28261 attaaacgta tataaatctg attttccttt aaaattaaat ttatatttat tatcttcctc
    28321 tattgcaatt cctttaaact cttttcttcc agatttaaaa atcacctcta actcaataaa
    28381 aaaatcccta ttatcaaggt ttctttgagt aatccccata aaaaacatat ttagttttcc
    28441 tatactctgt ttattcatca taatctccct tttaacttat atctttaaat cttattaatt
    28501 aatattattt gtttctgaat tatttatttc ttcttttaaa tcgttcaatc tatttttaaa
    28561 actttctatc tgcttatctg ataaatcaag atattctgtt atcttcccta tatatttagg
    28621 gcttttagta tttattaatt tcaaagcact ttccatagat ttttctctaa gctcatatgc
    28681 tttctttacg tcatctctag cattactcaa tgtttctgta tacttctcct tagaaagttc
    28741 tggaatctgc atattttcat attcttttac aacttcttta cacttgtttc tggcatttgt
    28801 aacatctttt tttatactag ataatttagt tatatttttt gctatatcct tagtgtaagg
    28861 tatacattct ctcattggaa ctaaagctgc tctttctata ttataaattt ctttcttaaa
    28921 aattcctata ctttccatat ctattttttt tatatcttca ctattttttt ctttacttgt
    28981 atatttatta ttttcttttg tattatcttc ttgtttttct gatttattat tcactgaaac
    29041 attactatca gaattattc
//
LOCUS       DAELNY010000032        29007 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.032, whole genome shotgun sequence.
ACCESSION   DAELNY010000032 DAELNY010000000
VERSION     DAELNY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.032"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            41..910
                     /locus_tag="KPE55_001204"
     CDS             41..910
                     /locus_tag="KPE55_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0492298.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1136..2002
                     /locus_tag="KPE55_001205"
     CDS             1136..2002
                     /locus_tag="KPE55_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0492299.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            2194..2391
                     /locus_tag="KPE55_001206"
     CDS             2194..2391
                     /locus_tag="KPE55_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492300.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            2394..2837
                     /locus_tag="KPE55_001207"
     CDS             2394..2837
                     /locus_tag="KPE55_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492301.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(3024..3111)
                     /locus_tag="KPE55_001208"
     tRNA            complement(3024..3111)
                     /locus_tag="KPE55_001208"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3075..3077),aa:Leu,seq:caa)
     gene            3263..5911
                     /gene="polA"
                     /locus_tag="KPE55_001209"
     CDS             3263..5911
                     /gene="polA"
                     /locus_tag="KPE55_001209"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG0492302.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            5920..6522
                     /locus_tag="KPE55_001210"
     CDS             5920..6522
                     /locus_tag="KPE55_001210"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG0492303.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            6515..7069
                     /locus_tag="KPE55_001211"
     CDS             6515..7069
                     /locus_tag="KPE55_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0492304.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            7069..8784
                     /locus_tag="KPE55_001212"
     CDS             7069..8784
                     /locus_tag="KPE55_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0492305.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            8857..9069
                     /locus_tag="KPE55_001213"
     CDS             8857..9069
                     /locus_tag="KPE55_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG0492306.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            9270..9869
                     /locus_tag="KPE55_001214"
     CDS             9270..9869
                     /locus_tag="KPE55_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492307.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            10097..10468
                     /locus_tag="KPE55_001215"
     CDS             10097..10468
                     /locus_tag="KPE55_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0492308.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            10600..11874
                     /locus_tag="KPE55_001216"
     CDS             10600..11874
                     /locus_tag="KPE55_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0492309.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDIVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQPNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            11985..12929
                     /locus_tag="KPE55_001217"
     CDS             11985..12929
                     /locus_tag="KPE55_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492310.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            13145..14482
                     /gene="rsxC"
                     /locus_tag="KPE55_001218"
     CDS             13145..14482
                     /gene="rsxC"
                     /locus_tag="KPE55_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG0492311.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            14505..15482
                     /locus_tag="KPE55_001219"
     CDS             14505..15482
                     /locus_tag="KPE55_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG0492312.1"
                     /translation="MENKLIVSSSPHMRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            15482..16051
                     /locus_tag="KPE55_001220"
     CDS             15482..16051
                     /locus_tag="KPE55_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG0492313.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            16064..16657
                     /locus_tag="KPE55_001221"
     CDS             16064..16657
                     /locus_tag="KPE55_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG0492314.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            16672..17247
                     /gene="rsxA"
                     /locus_tag="KPE55_001222"
     CDS             16672..17247
                     /gene="rsxA"
                     /locus_tag="KPE55_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG0492315.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            17263..18234
                     /locus_tag="KPE55_001223"
     CDS             17263..18234
                     /locus_tag="KPE55_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG0492316.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            18454..19038
                     /gene="maf"
                     /locus_tag="KPE55_001224"
     CDS             18454..19038
                     /gene="maf"
                     /locus_tag="KPE55_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG0492317.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            19083..19742
                     /gene="radC"
                     /locus_tag="KPE55_001225"
     CDS             19083..19742
                     /gene="radC"
                     /locus_tag="KPE55_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG0492318.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            19863..20873
                     /locus_tag="KPE55_001226"
     CDS             19863..20873
                     /locus_tag="KPE55_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0492319.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            20883..21776
                     /gene="mreC"
                     /locus_tag="KPE55_001227"
     CDS             20883..21776
                     /gene="mreC"
                     /locus_tag="KPE55_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG0492320.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            21791..22279
                     /gene="mreD"
                     /locus_tag="KPE55_001228"
     CDS             21791..22279
                     /gene="mreD"
                     /locus_tag="KPE55_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG0492321.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            22291..25269
                     /locus_tag="KPE55_001229"
     CDS             22291..25269
                     /locus_tag="KPE55_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG0492322.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            25367..26050
                     /locus_tag="KPE55_001230"
     CDS             25367..26050
                     /locus_tag="KPE55_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG0492323.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            26074..26871
                     /gene="minD"
                     /locus_tag="KPE55_001231"
     CDS             26074..26871
                     /gene="minD"
                     /locus_tag="KPE55_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG0492324.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            26889..27173
                     /gene="minE"
                     /locus_tag="KPE55_001232"
     CDS             26889..27173
                     /gene="minE"
                     /locus_tag="KPE55_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG0492325.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            27258..28388
                     /gene="rodA"
                     /locus_tag="KPE55_001233"
     CDS             27258..28388
                     /gene="rodA"
                     /locus_tag="KPE55_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG0492326.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            28429..28842
                     /locus_tag="KPE55_001234"
     CDS             28429..28842
                     /locus_tag="KPE55_001234"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG0492327.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT        11455 a         3132 c         5634 g         8786 t
ORIGIN      
        1 tatataatta agacaatact agtttgggga ggataaaatt atggattata aaacaatcat
       61 attaaataga aagtctgtta gagaatataa gaacacagat attaaagaag aatatcttag
      121 tgaaattaag aaatactcag aatcttgtaa gaaactagtt ccagaaatag atgttgatgt
      181 aagaattatg aatagaagtg aagtttttaa taatttagat aaagtagcag ggtacaaagg
      241 aaatatgata gattctccaa gttatattat aatattatct gacataaaag ataattacat
      301 tgaaaattca gggtacatag gagaaagtat aatctttaaa gtaacagaac ttggaattgg
      361 ttcttgttgg gttacttttg aagatggaaa aaaggtaaaa gaaaaacttg gaattaattc
      421 agataaagag gtaacaggaa ttatagctat tggatatgga gaaaatgtaa gtaatgctaa
      481 agtattaaat gctacaaaaa ctgggcaaaa ttattcaaaa tctgatatgc aagttgtaac
      541 agataagggg tctactagac taggtgtaga agatgttgtt tatatgaacg aatggggaaa
      601 tgttgcaagt atagaaatct tagaagaaag agcacttctt gatgcatttc actatgcaag
      661 attagctcca tctacattaa atagacaacc atggagattt atagttgatg gaggcactgt
      721 agttttagct gttagaaaag atggtcatac taatttatat gaagaaaaaa tagacattgg
      781 tatagttatg ctttattttg caacaataat aagtgcaact atgtttgatt taaaatggaa
      841 tttaggtact ccagacaaag attataaagt gccagaagac tataagatag taggatattg
      901 taatatataa gatagtgtaa tacttaattt aactacactt aaattaattt ttataaaaag
      961 ttcaataaca ttttacaata taatgttatt gaactttttt attgtataat acaattttta
     1021 tatttattgt attgattgaa aaatgcttat aaatttaaat gcttacaatt ttgtgcatga
     1081 atagagaggt atattatttt attaaactgt ataatttgct tggaggtaat atcaaatgga
     1141 atggataaat aaattaaatc aagctattac ttatattgaa gaaaatctcg aaagtggagt
     1201 ggattatgca gaagcagcaa aaattgcatg ttgttcaacc tttcattttc aaagaatgtt
     1261 ttcatatatt gcagaagtac cattatctga atatattaga cgaagacgta tgactaaggc
     1321 tgcatttgaa cttcagaaca gtaacatcaa aattttagaa ctatctgaga agtatggtta
     1381 cgattcacca acatctttta atagagcttt tcaaaatata cataacattt ctccatctgc
     1441 agctagagca aagggtgttg ttttaaaagc atatcctaag atgacattaa gcatttatgt
     1501 aaaaggaaat atagaaatgc aatatcgtat tgtagaaaag aaagggtttc gtattgttgg
     1561 tataaaagaa agtatgaaca tgattgtaga agagtgcttt gagaaagttc ctaagttgtg
     1621 ggctaaatgc ataaaaaatg gaacaattga taaactttca gagttaataa ataatgaacc
     1681 ttgtggattg cttggagtta gtgtgtgtac aaatagtaag ggtttagatt attatattgc
     1741 tgctccaaca gataagcctg tattagaaga tactcatgaa tacttaatac catcaggcac
     1801 atgggcagta tttgaatgtg tttctccaat gccaaatgct ctggcaatac aggaattgca
     1861 aaaaagaatt attactgagt ggttaccaag ttctgggtat gtgtatgcta atttaccaga
     1921 tattgaactt tatcccaatg gcgatatcaa tgctcctgat tatactacag aagtttggat
     1981 tccaattcag aaaccaagtt aaaattaata aataatatgg aattaataaa taacattaaa
     2041 attaataaac aacatgaaat tctaaaatta caggctgaaa atagtctgtt atttttttgt
     2101 aaatatggaa agtaagcttt acataaatgg aatatagttg tataattatg gaaagtagac
     2161 tttccgtagt tgagatatta aagggggata gatgtgaaaa atagattaga agaaataaga
     2221 aaagaaagag gcataaaaca agaagaactt gcttctattt tgcaagtatc aagacaaact
     2281 ataggttcat tagaaaatgg taggtataat ccttctatca tcttagcctt taagatagct
     2341 agatactttg atatgagtat tgaagaaata tttatttatg aggaggagta aaaatgaaaa
     2401 agaatatgtt atatgttgga attatatatt ttatttgtgg aattttactt gttttattgg
     2461 ctacatttac tgagttttca tttgaggctt ttatttgggg attatctgga gctgctttag
     2521 gtccaggtgt atgtatgatt cttcaatata tgtattggag taaacctgaa agagcagtag
     2581 attatgaaga aaagataaaa aatcaaagga tagagatgaa tgatgaaaga agaattatgt
     2641 tacgtgataa agctggacgt attactaatc aaatcatgtg ctatgttctt gtaattctta
     2701 tatttattgt ttctatattg agtgtatttt ctgtaatggt aataagcaag tgggttttag
     2761 ttgttcttgg attgcttatt ttatttcaat ttatttgtag tgttgttgta tacaataaat
     2821 tagataaaag gttgtaataa gttatgatat aaaatagaaa attatataaa agtaaattag
     2881 tttttaaatt aatgctagaa gataaattta tataattata taaattttaa aaagacacag
     2941 taatttcaaa aaataactta aaatttaaat caaaaataaa aaaactccta atctattgaa
     3001 taaatagctt taggagtttt taatggtgcc cgaggccgga ctcgaaccgg cacggagtat
     3061 tagtccggtg gattttgagt ccactgcgtc taccaattcc accactcggg catgtcatgt
     3121 atattattat agcaaaaaaa tatattgttt gcaacatatt taattttaat atactattat
     3181 tattatggga atattaaata acactatatt ggtatataat atatatgtta caaaaataga
     3241 taatttttag gagtgatata atttggaaaa aaaattaatt ataatagatg gaaattcaat
     3301 aataaataga gctttttatg ctcttccaga aatgaataat aaagaagggt taaagaccaa
     3361 tgctatatat gggtttacta caatgctatt taaaatgata gatatatata aaccaacaca
     3421 tataagtgta gcttttgaca gaaaagcacc tacatttagg catttagaat ataaagatta
     3481 taaggctgga agaaagggta tgccagatga attggcagaa caattacagc cattaaaaga
     3541 ccttttagat aaatttagaa taaatagact agaaatagat ggttatgagg cagatgatat
     3601 tataggtact gtttctaaaa aagcagaaga aaatggatat aaggtataca tagtaactgg
     3661 agataaagat gctattcaac ttgcttcaga taatacaact acccttataa cgaaaaaagg
     3721 ggttggagaa gtagaagaat acaactttaa ttctgtaatt gaaaaatatg agatgactcc
     3781 aacacaattt atcgatttaa agggtcttat gggagacaag tctgataata tacctggagt
     3841 acctggaata ggtgaaaaaa caggtataaa gcttataaaa gagttttcta gtatagagaa
     3901 tctaatagag aatacagata agcttaaagg tagtgtaaaa aagaaaatag aggaaaacaa
     3961 agaaatagct attcaaagta aaagacttgc aacaattata agagatgtgc caatagaagt
     4021 ggatttagat agtatgattt ttggcgatta tgataaagat gaacttttag ataaatttag
     4081 atactttgga tttacaagtc tcttatcaag attagttgat ttggtcgatt cagatgacac
     4141 agaacacgta gaagaaaaaa tagaaatatt aaaattaaaa gatatagaaa aatttataga
     4201 tgaagtaaac aaaaaaggac aagttatttt aaaaactgtt agaggacaag gtaatatact
     4261 tgaaaaaaat ataatatata tttttataag tgtagatgga gaaaaaatat attatgtaga
     4321 ttcaaatgaa gtgtcaaagt tagatagtat attttcaaat aatgaaatta aaaagtatgg
     4381 atataattta aaagatgatt atatatcttt aaaaccatat aatataaact tagaaaatat
     4441 gtattttgat ataacaatag ctgagtactt aatagattca acttcatcta cctcatatga
     4501 ttgtagtgca atagctatga agtatttatc aaaaaaggtt aaatcaaaag aagaattatt
     4561 aggaaagggt gttaaatcta aaaactatga ggatttagaa tttgaggatt tagcagagta
     4621 tatgggtcaa ataatatgca cagttaaaga aacagttcct atgatggaaa gaagtctaat
     4681 ggatatgagt atggatggtc ttttttatca tgtagagatg cctttagtag aagtcttagg
     4741 tcatatggag tatgaaggta taaaagtaga caaaagtata ttggatgaat tgagtgttga
     4801 atttaaagaa ataatagcta cattagaaaa ggaaatttat gaattatcag gagagccatt
     4861 caatataaat tcaccaaaac aattgggtgt tatattattt gaaaaattag agcttccaat
     4921 aattaaaaaa actaaaactg gatattcaac taatgcagat gttttagaaa aattaagaga
     4981 taagcatcca ataatagata agataactga gtatagacaa atagtaaagt taaattctac
     5041 ttacgttgaa ggacttttaa atataataaa tccagaaagt aatagaatac attcatcatt
     5101 taatcaaact ataacgacta caggaagaat ttcatctaca gaaccaaatc ttcaaaatat
     5161 acctataaaa atggaaatgg gtagaaaaat aagaaaagtg tttattgcag atgataactg
     5221 taagttggtt gatgccgatt actcacaagt agagctaaga gttcttgcac atatgagtca
     5281 ggatgaaaat atgatagaag catttaaaca tcatgaagat attcatacca aaacagcttc
     5341 acaagttttt aatgttccta tggaagatgt tactagtgaa ttgagaggtg cagctaaagc
     5401 tgtaaacttt ggtattatat atggtaaaag tgactttggt ctagctgatg atttaaatat
     5461 acctgttcca aaggcgaaag aatatatcga aagttatttt gctaaatacc ataaaataaa
     5521 agagtttatg gacacaacag tagaaaaggc tagtgaggat ggatatgctg taacaatttt
     5581 aaacagaaga agatatatac cagaaataaa gtcaagcaat tttatggaaa gaaatagagg
     5641 aaagagattt gcaatgaatg caccaataca aggaagtgca gcagatataa taaaaattgc
     5701 tatgataaat gttcataaaa aattggaaga gaacaatttg aaatcaaaac ttatactaca
     5761 agttcacgac gagttgatag ttgaagcaat tgatgatgaa atagatatag ttaagaaaat
     5821 agtaaaagaa gaaatggaaa atgctgtaaa tatggatgtt cacttagatg tagatttaaa
     5881 tgtaggtagt tcttggtatg agacaaagta ggtgatttta tgttgatatt aggacttaca
     5941 ggaggaatag gctgtggaaa aagcagccta tcaaatatat ttagaaatct taacattcct
     6001 atagtggatg ctgatattat atccaggaag atatttgagg ataagttgtt attggaaaaa
     6061 gtatttgtcc attttgggca aagtataaaa aatgatgatg gaactctaaa tagaaaagct
     6121 cttgggaaaa tagtttttag tgatgaagaa aaacttaagg aattaaataa cttgactcat
     6181 ccacgaataa gagaaaaaat aataagtgaa atagaaaaat tgagaaaaaa aggcgaaaat
     6241 atagtagtgt tagatgcggc aatattggta gaaagtggtt ttttagatat ggttgacaag
     6301 ctattagtag ttacttgtaa acaagaagta caaattagta gaatacaaaa gagagataat
     6361 tgcagtgagc aggaagcact tagtaggata aattcacaga tgagtcagga agaaaaatcc
     6421 aaatatggtg attatataat agataattcg ggaacaataa ctgaattaga aagtaaagca
     6481 cataaattta ttgagtacat gaaggagaat tggcgtgaat agtaagaaag tattgattct
     6541 atctattttt ataatcttat ttggggcact attaatggaa agcaaagtaa tacataaatt
     6601 tttatatcct aaaaaatatt cagagtatgt agaaaagtat tcgaaagaat ttaatttaga
     6661 tgaaaatata gtttacagtg ttattaaagc cgaaagtaag tttaatagtt ctgctgtttc
     6721 aaaaaaggaa gcaaaaggat taatgcaaat attagacata actagagatt ggggagcaga
     6781 ggaactaaat ttaaaaaatg tggatatttt cgacccagag actaatataa gacttggctg
     6841 ttggtattta agtaagttat acaaagaatt tggtaaatta gatttagtga tagctgcata
     6901 taatggtggt tcaggtaatg tgaaaaaatg gttagaaaat aatgaatata gtaaagatgg
     6961 cgaaaatcta catgatatac cttttaagca aacttcaaaa tatgtagaaa aagtaaaaaa
     7021 taattacgaa cattataata agatatatgg caagaaagga aaaaactaat gaagagaata
     7081 aaagtcttaa cagtcgtttt agctataact cttatggtag ctggttgtag taatactaaa
     7141 acaaaacaaa gtagtagtga ttcaagttta caaagtcaaa gttcgataga tgctaagtat
     7201 ataaacttaa caatggtaac tccaaaaact ataaatccta taactaatac tgataagtct
     7261 gttggttata taatgaattt agtttatgat agtttattta ctatagatga gaactacaat
     7321 gtaattcctc aattagttaa ggagtataat atagctcagg atggaatgag tatagatata
     7381 aagctaaaag atgcaaagtg gcatgatgga aaaaatgtta cttcaaatga tgtaaactat
     7441 actattggtt taattcaaaa aagtgcagat agtccatata atgaatttac taaaaatata
     7501 gcttctgtga atattaaatc agataaagat tttacaataa aatttaaagc tagatatgct
     7561 ttttcaattg atagtttaat ttttccaatt gtatctcaaa atcatttaga ttcaaaagat
     7621 gtaaatgaca agaataataa tatgatagga aatgggaaat ataaaataga atcatataca
     7681 gaaagagaag gaatggtttt atctgtaaac aaagattact atgaagaagt tccaaaaacc
     7741 atgaagaaca taaaagtagg tatggtgcct aatgaagatg caaggacatc tatggttatg
     7801 gctcttgata gtgatataac caatgttaca ttaaatgatt taagtaagtt tcaagaaaaa
     7861 gagtttaata taacaaaata tcaaggaaga gattatgagt gtgtattatt taattataat
     7921 aatccttttt tcaaagatgt taattttaga aaagcaattg cacactctat agataaagat
     7981 agaattatta gtgaaggtta tatggatgat gctactccag ttaattttcc acttaattca
     8041 aaatcaaaat actataatag tgaaatgaag gatttagagt ttaataaaga taaggcgata
     8101 gagtgtcttg ctaaggtaga atatgcaaat gtaaactcag taaatcaaaa tgacaataaa
     8161 gtaaaaaatc agaaaaaatc agctaaaaag aactcaaaga agcttacgcc agaagaagaa
     8221 gcaaaagcta aaaaagcaga agaagataga atcaaaaaag aagctgaaga gaaaaaaaat
     8281 agagaaaaag aggaagtaaa gaaaagtctt tctgaaatga acttaaagat aatagtaaac
     8341 agagataata gtgagagaat aaaaactgca aacataataa atgaaaactt aaaagctata
     8401 ggaataaata gtacagtaaa tcagctgtca gataaggata tggaaaatgc tcttaattct
     8461 aaaaattatg atttagcatt agttggatgg aaactatcaa ttataccaga tgcaagtagt
     8521 attatagcga gtagtggata tactgatgat aagttaaatg gatatatgtc tgcacttaca
     8581 agttctacgt ctgaaattga aacaaaaaaa gcatacaaag atgtacagac atatataaaa
     8641 gataatgcaa catttataag tttagctgtg agaaacaact atattgtaag taatagtaga
     8701 ttaaaaggta aaattactcc aaatgatttt gatgtatatg agggaatatc taatttagat
     8761 ataaaatcga atgaatcaaa ttaaatgatt aatttctatt taaaaagtat aaactatatg
     8821 atagaatacc cttatagggt ataaggaggt aattaaatga agaagaaact attagtagaa
     8881 ggtatgagtt gtggtcattg tgtaaaccat cttaaaactg cattaacaga agatatagat
     8941 ggtattgaag ttttagatgt agatttagaa aataagtgtg catctgtaga tatgaaagat
     9001 gatgtatcta ttgaaaaatt aaaagaagtg atagcagaat taggatttga actaaaggga
     9061 attgaataga aattgataaa ctaaaaaaag acctctattg ggggagtaat agaggtctaa
     9121 aaatggggga gattgagtat gtttgatttt acattactat tatctcccct tttaaattaa
     9181 tatatacata aaaagtaaaa aaattttgta atgactcaca tttcttgagc tttttgaata
     9241 tactataaat agctaaggga gggagtatca tggaatctag gggatataaa gaggatttaa
     9301 aaaaaatgat acttgatatg gcccacaaag aacttgagag ttatgtatca caaaaaggag
     9361 caaaaaaata ctttgaaggt aacttggata agattattaa taataatata aaaaaattca
     9421 atcaaagtat tggtcttaat agagtatatt taaaaatgtt aaaaaagtgt gcatatcaag
     9481 aaagtgtaac ttcaatatca agaactataa agaaagaagc attatttaaa tcaaaaaaag
     9541 agctatttgc tttttcagag tacttaaaac ttgatattaa caagcgactt tcatataatc
     9601 aaattttaag aaaaatatca agatatatat actacaatag aagttcatat gctcaaaaat
     9661 acgttgtttt caaaagagga gatgaagaat atcttttaga accagagaaa ataaaagatg
     9721 aattaatatc aagctatagg tcaaagacta gagaagatat gaggtcaata gcaaaacttt
     9781 tggatataga agtagaagat gattataatg ctgaagatat tagaaaaaaa gtaataaatt
     9841 atattattaa agaaaaactt aaaagttaga gttttaaaga ataagttctg ccaatttgca
     9901 tgattaagta taacttgggt ataaattttt tatgcatttg aaagagctta ttcttttttt
     9961 gacatattct tttaaataat ttaccatgat ttggtaattg gtcatcatca ttcaaatttt
    10021 taacttaata tgatttaaat aaatttataa taatgatata atataaaaaa aataaaaatt
    10081 ggagagtgat ttcaaaatga agtttaattt ttgtcataac aatttcaatg ttactaattt
    10141 agaaaaaagt ttagattttt ataaaaaagc tttagggctt aaagaggtaa agcgcaagga
    10201 agcagaagat ggaagtttta tattagttta tttaggagat ggcattacta gtcatacatt
    10261 agaattaact tggcttagag attgggatag accatataat ttaggagaca atgaatttca
    10321 tctggcttta gaagttgagg agtttgatga ggcaaaaaaa cttcataaag atttagattg
    10381 catatgtttt gaaaatgaaa gtatgggaat ttattttata gcggacccag ataattattg
    10441 gattgaaata cttcctaaaa atcactaatt ttaaggatat gtttataatt tctttacaat
    10501 taataagaaa ataagttatc attaaattgt agggactaaa aatggataat aatgttatga
    10561 atttatgact ataaaaattt gttgggggga gtgatagtag tgaaaaaagc tatagcagct
    10621 ttaggaattg gggcagtagc agtatctgtt agctccataa atgcaagtgc tcttgaaaag
    10681 ggaacagtaa cagcaagtgc cttgaatata agaagtggac caagttctga ttgcgataag
    10741 gtagcaaaat tatacaaggg aaaaactgta gagattttag aaaagtctaa tggatggtat
    10801 aaagtaaggg tatcgagttc tgttgttgga tggggaagtg caaaatacat atcaacaagt
    10861 ggttcgtctg aaggtacatc aagtcaaaat aatccaactt caagtgggac aactattagt
    10921 ggaaatggta aagtaaatgt gagttctaga ctgaatgtaa gaagtggtgc aggtactaac
    10981 tattcattag ttggtaaagc aaataatgga gatatagtaa agttactaga acaaagcaat
    11041 ggatggtata aaataaaact atcaaatgga gttactggat gggctagtag ccaatatatt
    11101 tctaaaacat cagaagatgt tggaacgaac aattctagta attcaaatag tactaataat
    11161 tctgataaaa aaccatctag cgaagaatca attgaaggca agaatggtaa ggtaacatct
    11221 gctgtgagtt taaatgtaag aagtggtcct gggacaagct attctataat aggaaaatta
    11281 aatggtggag atgttgttga attaaaagct aaaagtaatg gttggtataa ggtaaagtta
    11341 tcgagtggaa caattggatg ggtgagtgca agttatattt ctgagactaa tgaaggtaca
    11401 aaagaaaaac caaattcatc atctaaccaa aattctcaac ctaatagtaa ctcaaagcca
    11461 tcatttactg gaaattcaga taaatctaca gcaaaaggtt caacaatcgt agattttgca
    11521 tatactctta ttggaatacc atatcaatgg ggagcttcag ggcctgataa atttgactgt
    11581 tctggattta cacagtatgt ttttaaacat tctgtaggcg tttctattcc tagagtttct
    11641 agagaacagg ctaattttgg aagtgcaatt tctatgggga attatgcacc aggagattta
    11701 gtttattttg acactgatgg tgatggaaca actaatcatg taggtatata tgtaggaaat
    11761 agtaaattta tccattgtag tggtactcag actaatccta ataaggttaa agtggataat
    11821 ttaactagtt catattggtc taaagtatta cttggtgcaa gaagatttgt ttagtaagta
    11881 caatttctta ttaaaaaatt agaagaaata attatttata ttcataaatt gtgactctta
    11941 aaaatatact ttattaataa tataattttg ggagggacaa actaatgaat agaagaaaaa
    12001 cgatatacag aggatttaat aataatagac ataagattga tttacgtaaa tatgttataa
    12061 cgattgcttg cttgtgtcta attggctatt attcttatac aaaaataaaa gatagcaaga
    12121 tattggagta tgtatcggct aaaataccat ttctaaataa ttcatcagat ataacttata
    12181 aagatatatc tgatgaatta aattcaataa aaaaagggaa aaaatctaaa tcacaaacca
    12241 attcagatga taaacaagaa actaatccag aaaaagcggt aaataacact aaagagccag
    12301 aagaagtcaa attagctact atagaaggct gggatatgta tactattcaa gttgctgcaa
    12361 tagataataa tgatgatttg aaaaaaatac agacatcatt agttaataat gacatacctt
    12421 tttcagttat ggagaaggat ggagtaaaaa aaatacagac ctattcctct tttgatgaaa
    12481 atgatgttag aaagcagata agcagtgtaa gaaaggtgtt tccagatgca tttttatcac
    12541 acttagatgc acctatgttg tctttagagt atacaagtaa ctacgcttat atagagagta
    12601 tatctaaaga attaaataaa ttaataacaa actttaaaga agaatcaagt ttttggtcga
    12661 atgctgaaaa taatgtagat atggaaaaat ataatactat tttgacaaat agaaaggcaa
    12721 tatcacaaaa tatatcaaaa gaagctgaaa agattgatta ttctgaaatg cgtttattta
    12781 aagataattt aatagagtat gtaaagaatg tgaatgaaaa aattgataca gcttcaaaag
    12841 cagcaaatga agaaaaatat agtgtaagta aaagtctgct tttatcatcc atgcaggagt
    12901 actctatgtt tataaattct ataaaataaa atacagaaaa tttggacttt agaaagctat
    12961 atgaaaagga ttgtttaatt aagcaatcct tttcctcaat ttgacttaat atagaggtgt
    13021 tgatttataa aataaattat aaaaatacgg atattttttt aacaattgtt aagatttttt
    13081 aatatatata gtatacttat agaaggcaga aaaaatgttt ttttatagat aagggggtaa
    13141 aacaatgaaa ctcttaactt ttaagggagg gatacatcct ccatatagaa aagagtactc
    13201 taacacaaag gcacttgaaa aagctcaagc tccaaagata gtttacattc ctcttcagca
    13261 acatataggg gcaccagcta aacctattgt tgaagttggt gatgaagtaa agtttgggca
    13321 aaaaataggt gaacaacaag gttttgtttc atgtaacgtt cattcttcag tatcaggtaa
    13381 agttattgct attgaacaac atgaagtacc aggaggttca gctcaatgtg tagttatcga
    13441 aaatgacttt aaggaagaat tacatgagag tgttcaacca aaaggtcagt tagaggattt
    13501 gagcaaagaa gatatagtag gtatcataaa agaggcagga atagtaggta tgggtggagc
    13561 tacattccct aaccatgtaa aagtatctcc tccaccagat agcaaggcag aagtagttat
    13621 attaaatggt gcagaatgtg aaccatactt aactgcagac cacagattaa tggttgaaaa
    13681 tccagaagat gttgtgtttg gtttaagagc attaatgaag gtattagatg tcaaaaaagg
    13741 atttatagga attgaaacga ataaaccaga tgctatagaa gcaattcaaa atgtagctaa
    13801 agactatagc gaaatcgaag ttgttggcct tcaagtgaaa tatccacaag gagcagagaa
    13861 acaacttatt tatgcgtgta caggcaaaga agttccatct ggtggattac caatagcagc
    13921 aggtgcagtt gttgacaacg ttgccacagc agcacagata gctaagtcta ttaaaacagg
    13981 tatgcctttg gttgaaagaa tcactacaat aacaggtagt tgcatcaaag agccaaagaa
    14041 cctaataaca aaagttggaa cattagtttc agaaataata gaacaatgtg gtggatttaa
    14101 agaagataag aagattggta aggttataat gggtggacct atgatgggaa tagctcaata
    14161 tactactgaa atagcgacta acaaaggttc ttcaggaatt ttatgtttgg atgaggaaga
    14221 atcacgtact ccagatattc aaaactgttt aagatgtgga agatgtacag atgtatgtcc
    14281 atcattctta caaccacttt tcataagtgc atattcttta aaagatgact atgacacagc
    14341 tgaatatcat agagcaatgg attgtataga atgtgggtct tgctcattta tttgtccagc
    14401 aagaagacca ttacttcaat ctataagatc agcaaagaga gaaataggag caaagagaag
    14461 aaagcaagct gctcagaaat aaagaaagtt aagggggaaa aacgatggaa aataagttga
    14521 tagtatcatc ttctcctcat atgagaagta atgaagatac ttcatatata atgaaacaag
    14581 ttattatagc actccttcca gcagcagtag caggagtata cttctttaga cttaatgctt
    14641 tgagtgctat gtttttttgt atacttggta cagtaggtac tgaattttta tatcaaaaat
    14701 ttacgaagca aaaaagtact ataggagatt tttcagctgt tgtaacagga ttattattag
    14761 catttaacgt accagcatca cttccttggt ggatgtgtct agtaggagga atatttgcaa
    14821 tattagtagt taaaatggta tttggtggaa ttggatgtaa ctttgtcaat ccagcacttg
    14881 ctgcaagagc atttttatta gcatcattcc cagtagcaat gactgcttgg actcaaccag
    14941 gtgttaactg gataggtaaa aatttagatg cagttactac agcaactcca ttaagctttt
    15001 tgaaaaatgg agcagcagga ctggcagatc tttctagcaa tggaattagt cttgctgaca
    15061 tgatgatagg gaatattggt ggatgtatag gtgagacatc agcaatatta ttattattag
    15121 gtggagtata cctaatgtat aaaggcataa taaattatgt tataccagta ttttacatag
    15181 caactgtatt tatattaaca ttccttttag gtggatttaa tataactttc gcaatatatc
    15241 aactgtttgc tggaggactt atgttaggtg gattcttcat gcttacggat tatacaactt
    15301 cgcctatgac taaaaaaggt caaataatat acgctgtatt agctggtctt ataacaactg
    15361 ttataagaat gtatggtgga tatccagaag gtgtatctta ctcaatacta ttagtgaact
    15421 gtcttgctcc acttatagat aagtttgtta gaaatagagt atttggggag gtggctaaat
    15481 aatgaacagt atggtgagat taggtggtac tttacttgct ataagtgcaa tagctgcatt
    15541 agctcttgga gctactaatc aggttacagc tccagtaata gaacaaagaa atatacaagc
    15601 aaataatgaa cttagaaaag cagtccttcc agaagcaaaa gaatttaaag aaatgaagga
    15661 agatacttac aaaggccttg gagatggtct tatagctgaa gtgtatgaag gtttagatgg
    15721 ttctgaagtg gttggatata ctttaaaagc taaaccaagt ggatatggtg gagaaattga
    15781 agttatggta ggtatatcat ctgaaggtca agttacaggt gttgacattg gtaatatgtc
    15841 agaaacagca ggtcttggag caaaagctaa ggatgatgca tttaagggtc aatacaaagg
    15901 aaaaactgct gagcctcttg aagtagcaaa aggaagtaca acagcagata atcaaatatt
    15961 agcaatatca ggtgcaacta ttacatctac agcagttact acaggagtaa atgctgctat
    16021 agatgttttc aatagtgctc ttaataaata aggagggtta attatgaatt tagctaaagt
    16081 atttaaaaat gggctaatag atgagaaccc aacatttgtt caagttatag gtatgtgtcc
    16141 aacactagcc gttacaactt cagcaataaa tggaattggt atgggtcttt caacagcagc
    16201 agttttaata tgtgcaaatt tagttatatc tttaataaga aaaattacac cagataagat
    16261 aagaatacct atatttatag tagttatagc tacatttgtt acaatagtag gtatgctttt
    16321 aaaagcatat gttcctgctc ttgataaggc tcttggtata tatataccat taatagttgt
    16381 taactgttta atattagctc gtgcagaaag ttttgctttc aaaacaggag ctatgccttc
    16441 aatagttgat ggtgtaggtc aaggtcttgg atttactgtt gcacttacaa taataggtgc
    16501 agtaagagaa ttacttggaa atggtagttt atttggaatg acattatttg gagcatcttt
    16561 ccaaccagta ttaatattta tattaccacc aggtgcattc ttaacattag gattcttatt
    16621 tgcaggattt aataaattaa gaagtaaaaa agcatagtgg aggtgtaata aatgaattta
    16681 atacttttat ttttaagtat cgttcttgtt aataacgtta taacatctca attcttgggt
    16741 atatgtcctt tcttgggagt ttctaagaag gttgatacag cagtagggat gggagttgca
    16801 gttacgttcg ttttaactct agcttcaatt ataacttatt ttatacagat tttattagta
    16861 aaaactggaa ctggattctt acaaacaata gcttttatat tggttatagc ttctatagtt
    16921 cagttcgttg aaatggtaat tcaaaagatg agtccatctt tatatcaagc attaggagtt
    16981 tatttacctc ttataactac aaactgtgca gttcttggta tagctctagt taatgttcaa
    17041 aaaagttata atttagtaga aacaataata aacggatttg gagccggagc tggatttact
    17101 ttagcaatag taatattcgc aggtataaga gaaagactag aattagctga tataccagaa
    17161 gcatttaaag gtttccctat aacgcttata tcagcaggtt taatgtcaat agctttctta
    17221 ggatttacag gacttataaa gctataaaaa agaggggtga aaatggtgat acttacagct
    17281 gtattagttt taggaattat gggcttaatc ttcgggatag tactagattt tgcatctaaa
    17341 aagtttgctg tagaagtaga cgagagagtg gaagctatac taggagtact tccaggagcc
    17401 aactgtggag gttgtggatt tccaggttgt ggaggtcttg caaatgcgat agttgaaggc
    17461 aatgcaccag ttaatggttg ccctgtaggt ggagcagatg ttggtgcaaa agttggagaa
    17521 ataatgggaa taagtgctga agcaggtgag aaacaagtag caaaagttat ttgtaaagga
    17581 acttgttcaa gtgcaaaaga taaatatgag tatgaaggaa tatcagattg tagagcagca
    17641 aatgttttaa actcaggtgc taagatgtgt aaatttggat gtttaggtct tggaacatgt
    17701 aaagatgctt gtaaatttga tgctatttct atagttgatg gaatagcagt aatagatgaa
    17761 gaaaagtgtg taaattgtgg aaaatgtaaa gaagtttgtc ctaagggtat aataatcaca
    17821 aaaccagaaa gtcaagaagt agttgtagag tgtaatagca aagagtttgg taaagctgtt
    17881 aaagaaaaat gtactgctgg atgtattggc tgtggaatgt gtgtaaaagc ttgtaaattt
    17941 gatgcaataa tatttgaaga caaaatagct aagatagacc ctaataagtg tgtaggttgt
    18001 atgcaatgtg tagcaaaatg ccctacaaaa gtaatatcag gtgacataac taagaaaaag
    18061 aaagttacta ttgaccaaga actttgtgtt ggatgtacag tgtgcaaaaa acaatgtaag
    18121 tttgatgcaa tagagggaga attaaaagaa aaacataagg tagatgcaga taaatgtgta
    18181 ggatgccatt tatgtatgga aaaatgtcca aagaaagcta ttaaaatttt gtaatatgga
    18241 caaaataagg atatggaata atttccatat ctctaatgag tacataaaac agaggtatgg
    18301 aataatttcc atacctctat tttatgtata tttattctaa tggtaataaa tataacaata
    18361 gtgtaacaaa aaaggttcga catttctgtt gaatttttta caatatagtg atagactaaa
    18421 taggtacaaa ttacgtacaa atgaggtgaa taaatgaata taatcttggc atcagcctct
    18481 ccaagaagga aagaaattct agagaatacg aatgtaagat ttgatataat taaaaatgaa
    18541 attgatgaga ttatattgga aggtgaaaca ccaaaacatt tagttatgag attagctttt
    18601 gaaaaaagta tgtctgtagc atctgaacat aatgaagatg ttgttattgg agctgataca
    18661 gttgttgttt tagacaatgc tattttagga aaaccaaaag atgagtcttg tgcaagagat
    18721 atgttaaaaa gactatctgg aagagagcat caagtcataa caggtattag cttaataaac
    18781 ttgtgcgaag ataaaaaggt aattgactat gtaatcagca atgtgaaatt taaaacgtta
    18841 tcagagcagg atattgagga ttatctgaaa actaatgagt catttgacaa agcaggagcc
    18901 tatggtatac agggttatgg agctttgtta gttgaagaaa ttagaggaga ttattttaat
    18961 atagttggtc ttccaatttc aaggttaggc gacctattaa aaaaatattt tagtataaat
    19021 cttttttatg gagtgtgatt tatctgaaaa agtcatttaa ttcaaccatt aaggtaaaag
    19081 aaatggcacc agaggaaaga cccagagaaa agatgcttgc caaaggcgta aaaagcttat
    19141 caaatgctga gttgttggct atacttttaa gaactggcaa caagaataaa aatgctattg
    19201 aattagccaa ctatataata aatagagata ttcagggtat tagacattta gaagatatga
    19261 caatagagga gctgtgtaat atagatggaa ttgggttatc aaaatcaacc caaattaaag
    19321 cagctttaga actaggctct agagtagcta gttttaaacc tataaagtat aaaataagga
    19381 atccttggga tatacagagg tattatatgg atagcctaag gtatttaaaa aaagaggttt
    19441 ttaaagcggt tcttttaaac acaaaaaatg agataatatc tgatgtagat gtatctatag
    19501 ggactttaag ctcgtcatta gtccatccaa gagaggtttt taaagaagct ataagaagaa
    19561 gtgcaagtaa gataatagtg atgcataatc acccttcagg aagtgtagaa ccatcaagag
    19621 aagataaaaa tatcacatca agacttatta aatgtggaga aataattgga atagaaataa
    19681 tagaccatat aattattgga gatggattat actttagctt taaagaaaat atgataattt
    19741 gactttatat gtgataaaat aataggagaa tgttttaaac atgattctta atagtaggaa
    19801 ggagtaaaat catggctaag gaaaaaaaga aagaaaaaaa aggttttttt agttttaata
    19861 agatgacaaa ggatatggga atagatttag ggactgcaaa tacattagtt tacataaaag
    19921 gacaaggtat tgttgttaga gaaccatcag ttgtcgcaat aagagatgat agtaaagagg
    19981 tattagcagt aggtgaagaa gccaaaaaaa tgatagggag aacaccagga aatatagtag
    20041 ctataagacc tatgaaagat ggagtaatag ctgattttga cataactcaa tcaatgatta
    20101 gctattttat acaaaaagcg gctgataaaa aaggtgttgt aagtccaaga atagctatat
    20161 gtgttccttt tggagttaca gaagtagaaa agagagctat agaagaggct gcaagacaag
    20221 caggagctaa agatgcattc cttatagaag agccaatggc agcggctata ggggctggat
    20281 taaaagttga agaaccagaa ggtaatatgg tagttgatat tggtggaggt acttcagaaa
    20341 tagctgtaat atctttagga ggaatcgtta ctgctaaatc tataagaata ggtggagacg
    20401 aatttgacga gtctatagtg gcttatgtaa aaaaagaata taatcttatg ataggtgaaa
    20461 gaactgctga aaatgtaaaa attaacatag gttctacatt taaagatgat gaagaaataa
    20521 atatgcaaat aagaggtaga gatttaatct caggattacc taaaacaata gaaatatgct
    20581 ctacagaagt tagagaagca ttaaaagaac ctgtaagttc aatagtagat gcaataaaat
    20641 ctacactaga aagaactcca ccagaattag cctcagatat aatggaaaac ggaataatgc
    20701 taacaggagg aggcgctttg cttagaggtc ttgataagct tattacacag gaaactggta
    20761 tgtctgtaca aatagcagaa acacctcttg attgtgttgc acttggaaca ggtaaatctg
    20821 ttgaagacca agaaatcttt gaaaaagtat taatgatgaa tactaagaat tagaagttgg
    20881 tgatggcctt gagatttgac aagaacaaaa atgagaaaaa aaggattaat gttaaagtga
    20941 tagcaacggg cgtagttgct atcactttaa ttggaattgt gggtatatca attggtaaat
    21001 tttctagtgg tagtccagta aatttaggag tagcttcaga tgctattaca agtgtaggta
    21061 agggaataaa tgatggattt tcttttatca aaaatggttt taaagatgta gcaaatttta
    21121 aagataattc taaaaaggta aaaaaattag aagaagaaaa tgaaaaactt aaaaaaaata
    21181 tgatagcttt aaatgccaaa ctagataaaa cagaatcgct agaagagtta aaaaagacac
    21241 tgaactttgt acaagaagag tataaggcga caagtatttc agcaagtgtt gttggtaaaa
    21301 atgatggaaa ctggtatgag agttttgtca taggtgcagg aaaaaattca ggtgtaaaaa
    21361 aagaaagtat agttatgaat ggtagtggat tagttggcat agtttatgag gtttccaaca
    21421 attatagtaa ggcaatttca ctattggatt ctaaggcatc tgtaagcttt aaactagcaa
    21481 aggatgccaa tgctaaaggt acaattactc aaaatactac tttagataat aaagatagtt
    21541 ataatagtaa aggatatttg caaggatata tgtttgactc atcatataat gtaattcaag
    21601 gagatataat aacaacatca ggtcttggat tcttcccaga tggtatacca attggcgaag
    21661 ttgaaaaagt ggttgatgat aaagataagt cattaaaata tgtagttgta aaaccttatg
    21721 ttgattttaa gaatataaat gacgttgtag tcatagaacc aagaaatata ggataaaggg
    21781 gataaaaaca atgaaaaaag ttttactttg tctgttgggg attgtgctta tgactataga
    21841 aaatagtata acaaattata tagatatttt tggcataagc tttaatttag ttcttatata
    21901 tgtaaccata atctctcttt atttagatga attagaaggt gggattatag cagcaataat
    21961 agggcttatt aaagacataa ccgttggtgg aatttttggt gtaaatgcat taattttgtt
    22021 tattattgca tatgcaattg gatacatgag agatcagcta tataaaggta gttatattat
    22081 tactttcgtt ttaattttta taggcagttt aattgattca attgtcaata taggtacatc
    22141 tagtataatt tatcaaagct acaacatatc aacacttttt gtaaaaggtc tactagtagc
    22201 accaatagtg aatagtttaa ttggtttagt aatatatcgc ttatctaaaa gagcagtttt
    22261 aaaacttaaa gaagattagt tggtgatttc atggaagaaa ataaacaagt tgatagacta
    22321 aaaataatta agatagtcat gggcgtgata tttttagcta ttttagtaaa aataatctat
    22381 atgactacat ttaaatatga atattacaat gagttagctg agaataaaac atataaaaag
    22441 ttggcaatag aagctccaag aggagaaata aaagatagat atggaagact gttggcagga
    22501 aataaaaact tatttactgt tcaagtttca ggtaacgata ttaataagaa agatgcaaat
    22561 aagcattcaa gagcaaatga aatatcatta aaacttataa atttgctgga gagaaatggt
    22621 gaagaatatg tagatgagtt tcctatatat gtagaaaatg gaaagtacta ttatacatat
    22681 gacagagata ttagggaata taaatcagaa aatggtattc caaatgatta taatgctaag
    22741 gaaagttttt attacttggt ggacaaactt atatcagctg gaatattatc tcaagaagat
    22801 aaaagattgg atgcgacaag attacaggca aaactaaatg aaaatggata ttatccacct
    22861 attttagtta gtaaatggat gttcacagca gaaagagata agagagattg gttggctagt
    22921 tataagataa aagaaactaa actgagtgcc aaagaagcgt ttgaaaaagt aagaaatagt
    22981 gatgcattag aaattgataa aagtttgagt gatgaagatg caagaaaaat aatggtcgta
    23041 agggatttga taaaatcaaa aggatattct caatataatc cagtaactat tgctaaggat
    23101 gtgggagaaa ctacgattgc tcaaattgaa gaaagtgcta tggaccttgt tggtgtttca
    23161 atagcagtag aaccagtaag atattatcct aatgggtcat tagcttctca tatgttagga
    23221 tatgtaggaa aaatgccatc cactcaaata gaatcatacc ttcaaaaagg ttatgaaact
    23281 ggagatatgg taggtcttgc aggagtagaa aagagtaatg aaagtagatt aagaggaact
    23341 gatggttata aaatggttaa agttgatgca ttgggtagaa tcagtaaaga aattgaatct
    23401 aaaaaaccta aatcaggaga tactgtgtat cttacactag ataaagattt acaagaagtt
    23461 tcagataatg cattaaaaca gataatcgaa gttgcttcta aaggaggtac ttttaaaagt
    23521 aaatttgggg acaaaccaat tagtgcatat gcaggaaaag cacagtcagc agctcttata
    23581 gctattgatg ttaaaaatgg agaagtatta gcttcatcaa gttatcctaa ttatgaccca
    23641 aataaatttg caaaaggtat ttctacagag gattataaag cacttcaacc aaaaaatcca
    23701 aatgacttac tagcaggaag tccactttta aacttggtta cacaagggga atttcagcca
    23761 ggttcttcat ttaagatggt aacttcaatg gcagcgttag aaaatggtct ggatccaaac
    23821 tttactataa atgaccctgg ggttataatg ttaggtaaaa aatcttttgg tgactatgta
    23881 tggaaccatg gtagaggaaa ccatggtatg acaaacttat ataaagcaat acaagagtct
    23941 tgtaatatat atatggcaac aattggtaca ggtaaaacat ggccagatgg aaaaagcatt
    24001 ggtatagata tgaatgcaaa taaaatactt gagtatgcaa aattatttgg gcttgaccaa
    24061 aatacagggc ttcaagatga agtagaagaa agagcaggta aagttcctag cacagaagat
    24121 aaattgaaat caacacaagc tcttctaaag tcaaatttag aaagagaaat ggcaaatgat
    24181 tttgttgata taactagaga aaaaaatcct aaagaatatg agaaaagaat caatgagatt
    24241 gtaagctggg cagcagaaaa gaaaactcct ggtagagttg aaacaatgaa taggctaaaa
    24301 aaattggatg ttaaagaaga tagaattgaa gaagtagcag atttagcagt atttagttat
    24361 tttaattttg caaaatggag tactgcagat acattcaact tagctatagg acaaggtgaa
    24421 aatgcttata caccagctca aatatcaaga tatgtagctg ctatagcaaa tggaggtaat
    24481 ttagtagaat tatctgtagt tgatagagct gtttcaagtg attattcatc agttaaaatt
    24541 aatgaccaga agaaagtcga aaaaatacct tttaaaaatc cagataactt aaaagaatta
    24601 acaaaaggta tgaaattggt tgcaagacaa ggtacagcga agagtgcttt tgctgatttt
    24661 ccaatagatg tagcagcaaa aactggtaca gctgagaaaa gtgggaaaat acctacagat
    24721 aacgagtatg aatatttaaa aagtcatatg tcatcttata atgtcaacct aaatgatgcg
    24781 attaaactag ctgataagat gaaagcagaa aaagaaaaag agctttcatt agctaaggaa
    24841 aaggaaataa aaaagaaatt agaaaataaa gatttgaaag acgaagaaag aaagaaatta
    24901 gaggaagaat tagaagatgg agtaaaagtt agattagagg acactgataa ggtgaattct
    24961 tcatatctaa gaaaagctat aaaagaatta aatccaaaaa taacagatga ccaaatagat
    25021 agatttaaac aagattatgg ttcttttacg tggactgttg cttttgctcc agcagatgac
    25081 ccagaaatag ctgttgtatg tgtaattcct caaggtgatt caagtgtatt ctcactttta
    25141 ccaactagag aagttattgg aacttatatg ggattagagc caactaatag taaaaatgac
    25201 aacaagacga atgatgttaa caattcttct gacgaaaata taaatttcga gtcacaaatt
    25261 aatagatagc tcaatctatg tataaacttt agctttatat agaaaaaata aaagggttta
    25321 aagcgtattt gaagaatata aatgatatat cactctggag gttgttatgt ctttaagaga
    25381 aatttgtagt caagaactag tagagttcaa gggtaataaa agagggataa tagtaaatat
    25441 aaaaagagaa gctccatttg aggaaataca ggaaaaaata ataaacaaat tggaagcata
    25501 tgttggcttt tttaatgggg ctaaaattag taaaattaat agtgattgtt taactgacat
    25561 ggagattcta gaacttaaag aaggaattac ttctagattt gatgtggaat ttgttgaaga
    25621 tcaaaaaatt gaagaaaata gtaattttcc aacaaaatat gtcaataccc taagatctgg
    25681 tgaaaatatt gaatttgaag gtgatgtagt tatattgaat gatatgaaac ctggttcaaa
    25741 agtcctttca aaatcaaata cagttgttat gggagatata aatgctggtg ctaaggttgt
    25801 agcaggaggt aatgtttttg taatgggtaa aatagaaggt tttgtacatg caggtgcaga
    25861 aggcaatgaa tttgcatatg ttgttgctgg aaatcttaat cctaaaatat tacaaatagc
    25921 agataatatt gcagaagctc cagatgatga agaaaattat gaaagtgaat cagaaattag
    25981 cccagaaata gcctttgtaa gtaatggaag aattgtaatc gaaagctatt tgtcaaagtt
    26041 agacaaatag aaagaaaaat agggggtagt attatgagcg aagttatagt tataacatct
    26101 ggtaaaggtg gggttggaaa aactactacc gcagcaaact taggaactgc gctaagccta
    26161 gaaaataaaa aaacagtagt tgtggatgca gatattggac ttagaaactt agatgtagta
    26221 atgggtcttg aaaatcgaat agtttatgac atagtagatg tagttgaagg aacttgtagg
    26281 cttaagcaag ctctaataaa agacaaaaga tttgataatc tatatttatt accagcagca
    26341 caaactagag ataaaaatgc tgtctcagtt gagcagatga ttgacctgtg tgagaaacta
    26401 aaagaatctt tcgagtatat aataatagat tgtccagcag gtattgagca aggctttaaa
    26461 aatgcagtag ctggggcaga tagagctatt gtagttacta atccagaaat atcagcagta
    26521 agagatgctg atagaataat aggtctatta gaagcaaatg aaataaaaga gataagatta
    26581 gttataaata gaattagaaa tgatatggtt aagcgtggag acatgatgga taaacaagat
    26641 ataatagaaa tattagcaat agatttgtta ggtcttgttc ctgatgatga aagcataatt
    26701 atatcaacaa ataaaggaga gccagctata cttgattcta agtcacttgc tggtcaagca
    26761 tacaaaaata tcgcaaaaag gatactaaat gaagaagttc ctctacttga tcttgaagtt
    26821 gaagatggat tctttggtag acttaaaaaa atgtttagca tggctaagta gcaaaggagg
    26881 atacatctgt gttagattta tttagagttt tttctaacga ggctaaaact agtaaatctg
    26941 ttgctaaaga gaggttaaag ctagttttag ttcatgatag agtagattgt tcaccacaac
    27001 ttttagagat gataaaaaca gacattttaa aagtaatagc taattatgca gaaatagaag
    27061 atgatggtct tgaaataaaa atgtctaaat gcagaggtga gcatgatgac aaacctgtat
    27121 ctgcattagt tgctaatata ccactaaaga atataaaaga tagatgtatg taattagtgc
    27181 ttaaaagggg gatatcctta ttggtatatg ccccttttat ttaattatat gtttaaagat
    27241 agaaagacgg tgattctttg aaaaaaatca atattaatta taagtcgaca attaagttga
    27301 taaaacagtt agactggaaa cttatagtta cagtattagc tatctttatt tttgggcttg
    27361 ttatacttag tagtgcaaca catgctaatt caacaggttc atataatcaa ttgattaaac
    27421 aaggattagc ttttgtactt gggataggaa tgattatagt catattattt tttgactata
    27481 accttctagg caggtattat aaagcgcttt atataataag tcttattttg ttagccatag
    27541 ttttattacc tggaattggg acagtaaaag gtggagctag gtcgtggatt aacttaggac
    27601 cacttgactt gcagacatct gaaatagtca aattaacatt tgtactaagt tatgcaaaga
    27661 tacttgaatc caaaaaggat aaattaaata cgttaaagga agttatgcct gttgtagttt
    27721 attctcttcc atttatcgga ttattgattg cacaaccaga cttgggaaca ggaattgtat
    27781 tttgttgtat gatatttgca atgctgttca cagcaggtct tagctcaaag ttgataaaaa
    27841 gaggaataat catactatta gtgtcaatgc cactgatgta tttaatgatg gcagaccatc
    27901 aaaaggtaag aatagaagct tttctaaatc cagaagatgt aactctaaaa ggaaactatc
    27961 aagttatgca atctctgatt gcaattggtt ctggaggcgt aactggtaaa ggactttaca
    28021 atggaagtca aaatcaagaa gattttttac ctgtacaaga tagtgatttt atatttgctg
    28081 ttgtaggtga agaacttggt gtaataggaa tggcagtgtt gataatctta tttatgatat
    28141 tcctattaag gcttttagca attgcaagag atgcaaaaga tttttatgga accctcatag
    28201 tggttggtgt tatgggaatg tttggttacc agataatcca aaatataggt atgacggttg
    28261 cgttaatacc agtaactgga gtaacattac cttttgtaag ttatggagga agttcgttat
    28321 tgacatcttt agcaaactta ggattagtac taaatgtgtg tatgagaaga aagaaaataa
    28381 atttctaaat ttaaatacat ctaaacataa attgaggaga ttagatatat gaatatagca
    28441 ttagtagcac atgaccaaat gaaaaacact atggtcggat tttgtatagg ttatgaatca
    28501 attttaaaaa agtatggact atatgcaact ggaactacag gaaaaagaat aatggatgaa
    28561 actgaattaa atataaatag attagcatct ggtcctttag gtggagacca acaaataggt
    28621 tctttaatag taacacaaga aatagattta gttatttttt taagagaccc attgacatct
    28681 caggctcatg aaacagatat acaagcgtta ataagacttt gtgatgttta tcatgttcca
    28741 atagctacaa atctagcttc agcagaaata tttataaaag ctcttgatag aggagaacta
    28801 tcatggagag aagttagaaa aagcaaaagt caacgtattt aaaaaatatt aatattacag
    28861 tctaaagaaa cagatatata agtgtttaat gccctagtct ttctgttgga ataaatctag
    28921 tacctgtaga aatcttatga aagatttaaa tgatgaagag ctaaatttga gattaaataa
    28981 ttaggaaata gattatagat agagtag
//
LOCUS       DAELNY010000033        28948 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.033, whole genome shotgun sequence.
ACCESSION   DAELNY010000033 DAELNY010000000
VERSION     DAELNY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.033"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..955
                     /locus_tag="KPE55_001235"
     CDS             <1..955
                     /locus_tag="KPE55_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0492328.1"
                     /translation="LKKDNCSFEKVNTYLILSKNIYKSFGDVFIYNSKLDYIYKLYGD
                     LYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKDFKLAIEYYKS
                     MDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDILKDGLTKLYNR
                     RYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVTDIIRSVFIDI
                     TEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEHIYSKIKNYIT
                     ISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII"
     gene            963..2963
                     /locus_tag="KPE55_001236"
     CDS             963..2963
                     /locus_tag="KPE55_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0492329.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            3225..4484
                     /locus_tag="KPE55_001237"
     CDS             3225..4484
                     /locus_tag="KPE55_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0492330.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            4545..6332
                     /gene="iorA"
                     /locus_tag="KPE55_001238"
     CDS             4545..6332
                     /gene="iorA"
                     /locus_tag="KPE55_001238"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG0492331.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            6334..6909
                     /locus_tag="KPE55_001239"
     CDS             6334..6909
                     /locus_tag="KPE55_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG0492332.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            6962..8032
                     /gene="buk"
                     /locus_tag="KPE55_001240"
     CDS             6962..8032
                     /gene="buk"
                     /locus_tag="KPE55_001240"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0492333.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            8250..8951
                     /locus_tag="KPE55_001241"
     CDS             8250..8951
                     /locus_tag="KPE55_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492334.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            9011..9517
                     /locus_tag="KPE55_001242"
     CDS             9011..9517
                     /locus_tag="KPE55_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG0492335.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            9692..10714
                     /locus_tag="KPE55_001243"
     CDS             9692..10714
                     /locus_tag="KPE55_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0492336.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(10861..11169)
                     /locus_tag="KPE55_001244"
     CDS             complement(10861..11169)
                     /locus_tag="KPE55_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0492337.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(11229..11657)
                     /locus_tag="KPE55_001245"
     CDS             complement(11229..11657)
                     /locus_tag="KPE55_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492338.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(11880..13337)
                     /locus_tag="KPE55_001246"
     CDS             complement(11880..13337)
                     /locus_tag="KPE55_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0492339.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13755..14669
                     /gene="nadA"
                     /locus_tag="KPE55_001247"
     CDS             13755..14669
                     /gene="nadA"
                     /locus_tag="KPE55_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG0492340.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            14682..15983
                     /locus_tag="KPE55_001248"
     CDS             14682..15983
                     /locus_tag="KPE55_001248"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG0492341.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            15964..16800
                     /gene="nadC"
                     /locus_tag="KPE55_001249"
     CDS             15964..16800
                     /gene="nadC"
                     /locus_tag="KPE55_001249"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG0492342.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            17056..17388
                     /locus_tag="KPE55_001250"
     CDS             17056..17388
                     /locus_tag="KPE55_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG0492343.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            17550..17735
                     /locus_tag="KPE55_001251"
     CDS             17550..17735
                     /locus_tag="KPE55_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492344.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            17762..18847
                     /locus_tag="KPE55_001252"
     CDS             17762..18847
                     /locus_tag="KPE55_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0492345.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            18840..19298
                     /locus_tag="KPE55_001253"
     CDS             18840..19298
                     /locus_tag="KPE55_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492346.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            19333..20424
                     /locus_tag="KPE55_001254"
     CDS             19333..20424
                     /locus_tag="KPE55_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0492347.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            20429..20653
                     /locus_tag="KPE55_001255"
     CDS             20429..20653
                     /locus_tag="KPE55_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492348.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            20663..21226
                     /locus_tag="KPE55_001256"
     CDS             20663..21226
                     /locus_tag="KPE55_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492349.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            21187..21816
                     /locus_tag="KPE55_001257"
     CDS             21187..21816
                     /locus_tag="KPE55_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492350.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            21993..22745
                     /locus_tag="KPE55_001258"
     CDS             21993..22745
                     /locus_tag="KPE55_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492351.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            22758..23243
                     /locus_tag="KPE55_001259"
     CDS             22758..23243
                     /locus_tag="KPE55_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492352.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            23250..23399
                     /locus_tag="KPE55_001260"
     CDS             23250..23399
                     /locus_tag="KPE55_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492353.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(23536..24357)
                     /gene="yidA"
                     /locus_tag="KPE55_001261"
     CDS             complement(23536..24357)
                     /gene="yidA"
                     /locus_tag="KPE55_001261"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG0492354.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            24850..26544
                     /locus_tag="KPE55_001262"
     CDS             24850..26544
                     /locus_tag="KPE55_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG0492355.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      26876..26986
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            27090..27461
                     /locus_tag="KPE55_001263"
     CDS             27090..27461
                     /locus_tag="KPE55_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG0492356.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            27558..28505
                     /gene="trxB"
                     /locus_tag="KPE55_001264"
     CDS             27558..28505
                     /gene="trxB"
                     /locus_tag="KPE55_001264"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0492357.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            28609..28926
                     /locus_tag="KPE55_001265"
     CDS             28609..28926
                     /locus_tag="KPE55_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG0492358.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        11350 a         3056 c         5237 g         9305 t
ORIGIN      
        1 gttaaagaaa gataattgta gttttgaaaa ggtaaacacc tatttaattt tatcaaaaaa
       61 tatatataaa agttttggag atgtttttat atataattct aaactagatt acatatataa
      121 gttgtatgga gatttatata taaaatttgg taatatagat gaaggtataa gttgttatat
      181 aaaaggttta aatttagtta aaaagagttc attgaatctt agaaaaatat ctatatttta
      241 tgggctaata gcaaatggat atgagctaaa aaaagatttt aaactggcaa tagaatatta
      301 taaaagtatg gattggtatt ctgataggtg ggaaaaaaat aattcctata aaacatctca
      361 agcaattcac aaacaatatg aactcagaca aaaacaagag agtttaagat tactagtgga
      421 tgataataag aaattagaaa atgatatttt aaaagatgga cttactaaac tatataatag
      481 aagatatctg gaacagcagt tggatttatt taatgaaacc agagacaaaa atatggtcgc
      541 aatactggtc gatatagatt gttttaaaag ttataatgat aattatggtc atctagcagg
      601 agataaagtt atagttaagg taactgatat tataagaagt gtatttatag atattaccga
      661 acacataatc agatatgggg gagaagaaat tttaatatta gtagattcta tagaatcaat
      721 cggtgttgaa gagattgata atatagaatt atatattaaa agagttttca aacttcttga
      781 atttgagggt attgaacata tatattctaa aataaaaaac tatataacta taagtgctgg
      841 agtaagtata aaaagatgtc gtagtagaaa agatgttgaa atattaattg aagatgcaga
      901 taagaaccta tataaatcaa aaaaagcagg tagaaatcaa tacattataa tttaaaattg
      961 aggtgatttt gttgcgaaaa aaaataggaa ttatagcctc tgatattgaa ttaaaagaaa
     1021 gaatagaaga attatataga gaagatgtag aaaatggaac tataataatc gacatactta
     1081 acctagactt aatggaaaat caaggtcgta tacttgttga aaaaggtgcc caagctataa
     1141 ttggtagagg tggaggttat agtttagtaa tagatacagt aaatgtacct gttataccaa
     1201 tgaatatgaa gtcaactgac cttttaagag caatagaaat agcaaagaaa tattctaaaa
     1261 aagtagtatt gattcttgga gacaatgaag tttcttttga ctatgttggc tggagaaatg
     1321 tcataagtac tgaaataact gaagaatggt ttgaatctaa gtatgagata agaagtaaag
     1381 ttgtaaaata tatagaccaa aaagatgaaa ttgtaatcgt aggaggaggt ttggcatgtt
     1441 cttttgcaag gcaatatgga attgatagtg tatttgcaac tgctagtgat gaatctataa
     1501 gagaagctgt agaatattgt aaaaaattat tagatacatt aggtgaagaa aagtttaata
     1561 atgaagtgtt acgaaatata cttgatggta taaaagatgg agtaattgct atagatagta
     1621 atggcagtat aattttgtac aatgaaagtg caaaaaacat gcttaaagta gagagaaaat
     1681 gtgcacttaa taaatacatc ttagatgtat ttcctaaaat ggagtggatg ttagattgtt
     1741 tacatgaaaa agaggacgta gaagatagaa agataagaaa tataaataat ctaattgtta
     1801 atactaggac aacattgata aaagttgata atagtactta tggagtttta ggaataatac
     1861 aagatataac caagctacaa aacttagaaa gaaaaataag atttgacttg aatcaaaaag
     1921 gtctctatgc tagatatact tttgatgatt ttttatttaa agataaatta actaaagaat
     1981 ttattgaaga agctaaaaaa attggaaaaa gtgattatac aactttactt tatggggaaa
     2041 gtgggtctgg taaagaaatt atagctcata gtatacacaa tataagtaaa agaaaagata
     2101 ggccttttgt tgctataaat tgtgctacta tagcagaaaa tttacttgaa agtgaacttt
     2161 ttggctatga agaaggtgca tttactggtg ctagaaaggg tggaaaaaga gggctatttg
     2221 aacttgctca tggagggact ctttttttag atgaaataaa tagtctatcc tttaatattc
     2281 agacaaaact attaagagta atagaggaac gtcaaataat gagaattggt tctgattata
     2341 taatacctct ggatataagg attatagcag caacgaacga atctttaacc gaaaaaatag
     2401 ttatgggtac atttagagca gacttatttt atagattgag tagtttagag ataaatatac
     2461 ctcctttaag agacagaaga gaagatataa tacctttgtt taacaatttt gtaaatgaag
     2521 tattaaagga tgatggtctc aatggaataa acagtataga tgaaaatttt gttttgacta
     2581 aagatgaaat ggataagcta tataattata gctggcctgg aaatgtaaga gagttaaaga
     2641 caatagctca aaaatatgtt gtgacaggta aaattaaatt gagacaagat agagatttta
     2701 aaactaaaaa attacttcct aactctgaag tagacaaatt taattctgaa acgacagcaa
     2761 gtgtagaagt ccaagatgaa tctataaata taagtaagat aaatgatggt aagataagta
     2821 tagatataaa agaagtaaat aagtatgttg aagaaaaaat aataagtatg ctttttgctc
     2881 aaggactttc taaaaatgaa gttgcacaag ttcttggaat aagcaggaca tctttatgga
     2941 agaaatacaa taaaaatatt taaatataat tacatataaa ttattttgaa gtaatgttaa
     3001 ttttttaaaa caagtgttaa gaaaaattaa cattactttt ttgcaaattt ttattttttt
     3061 agacaggcat atatatttat aagaaaaata gttaaaacac ttaatttaat aatgaaaaaa
     3121 ataataattc taaacaaaat caattttaca taaagtggga tttattggca tagtaattgc
     3181 ttaatatata atgtaagagt aaatacaatt ttaggggtga tggtatgagt caaagtatgg
     3241 ttggtaagca tgcaatgtgg ccaaaagaaa atgatgttat atttagcata tctggcagag
     3301 ctcaagcagc agagaaggct tttggtatgg ataatgtaat aaatgcaaca ataggcgctc
     3361 taatggatga ttcaggaaaa ttgataacaa tgaaaactgt atatgaagaa tataaagcat
     3421 tagataactg tgagataggt gcatatgcag ctcttgaagg tcaaccagat tacttagaag
     3481 cagttaaaaa agtatttttc agagattact taccagaagg acatataaga gtattagctt
     3541 caccaggagg aagtggtgca ataaaattag ctgtatggaa ctatactaat gaaggagatg
     3601 aggtattaac atctgattgg ttctggagtc catatgtaag tatagctgaa gaggctaata
     3661 gaaaagtagt taattaccaa ctttttgatg aaaatagaag gtttaatttt gagtcgttta
     3721 aagaaaaatt tgtaaatata gcagaaaaac aaggtagagt ctttacaatt ataaacacac
     3781 cagcgcataa tccaactgga tatagtgttg cagatgatga gtgggataaa atattagact
     3841 tatcaaaaga agtcgcaaaa gataaagaca aaaagataat atttttcgta gattcagcat
     3901 atattgactt tgcaggtgat gatgatgtat gtagaaagtt ttttaagaaa ttttctaatt
     3961 tgcctgaaaa cgttctcgta ttggttggat tcagtatgtc aaaaggattt acagcttatg
     4021 gtatgagaat gggggctata atttgtatat cttcaagtga agatgtagct gaagagttcc
     4081 attactcatg tgttcattca tgtagagcaa attggtcaaa ttgtaataga agtgccatgg
     4141 cagtattatc taacattgta aatgacccta aaaaattcaa agaatatgag gatgaaaaag
     4201 aaatatacaa aaacatgctt actagaagag cagatgtatt tgtaaaagaa gctgaaagag
     4261 taggattaga aattcttcca tatatagcag gtttctttgt aagtatacca tgtgataatc
     4321 caaaagaagt atgtgaagaa ttgactaaac ataatttatt tgcagttcca ttaaaaatgg
     4381 gtttaagatt tgcagtttgt gcagtttctg aagataaatg taagaaagca ccaagtatta
     4441 taaaagaagc acttgaaagt ttagaagtaa aaataaataa ttaatttcat tgaacaaatt
     4501 gggtggacta tgaatttaat aattgtagat ggagggaatt ttatatgaaa caattgatga
     4561 caggtaatga agcaattgca cgtggagcct atgaggctgg tgttaaatat gcatctgcat
     4621 atccagggac tccaagtaca gaaatattag aaaatatagc aacatataaa gatgcaatag
     4681 ttgcagagtg ggctccaaac gaaaaggtag cacttgaggc tgctataggg ggttcaattg
     4741 caggagcaag aacaatggct tctatgaaac atgttggttt gaatgttgca gctgatccaa
     4801 tatttactta tgcatataca ggggtaaatg gtggtatggt acttattaca gcagatgagc
     4861 cgggaatgca ttcatctcaa aatgagcaag acaataggat gtatgcaaaa tttgcaaaga
     4921 tacctttatt tgagccatca actagtcaag aagctaaaga tatgataaaa gaagcttttg
     4981 aagttagtga aaaatacgat acaccagttt tatatagagt tacaacaaga ctttgtcatt
     5041 caaaaggatt agtggagtgc tatgatagag aagaagttga aattaaagag tatgttaaaa
     5101 atgctaagaa aatggtaact gttccagcaa atgctcaaat aagaaggggc gttgttgaag
     5161 aaagaatgga aattcttaaa aaattctcaa atgaaactga tttaaactac tatgaaataa
     5221 atgatacaaa aataggtgtt atagcctctg gtatgtgctg taattttgcc aaagaagttt
     5281 ttggaaaaaa tgcatcatat atgaaactag gatttacaaa tccattacct tatgaaaaaa
     5341 taaaagaatt tgcagaaaaa gttgataaaa tatatgttat agaagaaaat gacccattta
     5401 tagaagaaca aataaaagca tatggaatag attgtattgg aaaagatgta ataccaccat
     5461 atggagaaat gacaccagat gttttaagaa aagctatatt tggaaaaaca aatgatacta
     5521 tagaatacaa atctgaatta gtaacaccaa gaccaccaag tttctgtgca ggatgtccac
     5581 atagaggatt cttctatgaa cttggaaaga gaaaaaattt aatagttggt ggagatatag
     5641 gttgttatac tcttggattt gctcctccat ataatggaat agattatgtt gtttgtatgg
     5701 gttctgcttt tggaactgca catggagctc aaaaagttct taatatgaag gatgataatg
     5761 aaaaaagatt ggtaggtgta cttggagact caacattctt ccatacagga ataaatggac
     5821 ttttagatgt tgtttacaat agaggtaatt ctatttcggt tatattagat aatagaataa
     5881 ctggaatgac aggtcaccaa gaaaacccag gttcaggata tactttacaa ggtgctaaaa
     5941 ctaaagaagt tgatatagaa ggattagtta aagcttgtgg aatagaacat gtaagagtta
     6001 taaaccctaa taatttaaaa gaagtaaatg aagcactaga ttgggcttta gctatagaag
     6061 atgaaccatc agtaataata acaagatggc catgtgtact taaaaaattc tctaaagaag
     6121 atatagaaga atttaataat ccatttaaga ctaagtgtaa agttgaccat gataaatgta
     6181 taggatgtaa attatgtttg aaaactggat gtccagctct atcatttgat aaagagaata
     6241 agttatcaaa tatagataga aatcagtgtg taggatgtgg agtatgtgca caagtttgtc
     6301 caaaacaagc aatagttaag gaggaaaaat aatatgacta agagtatact tttggtaggt
     6361 gtaggaggtc aaggaactat acttgctagt aaattattaa ctatgggatt gatggaagct
     6421 ggatatgatg taaagatgag tgaaatacat ggaatgagtc aaagaggagg ttcagtttcc
     6481 tcacaagtta gatatggaga ttgtgttcat tctccagtta tagaaattgg tggagcagat
     6541 atattagtat cttttgaaaa aatggaagct ttaagatggt tcaattattt aaaaccagaa
     6601 ggaaaagctt tagtaaataa tcataggata gattctatga ctgttttaat cggaggagct
     6661 gaatatcaag aaagtgatat agatgcagag ttaagtagat taaatgcaaa agttataaat
     6721 gcagctgata aagctgaaga actaggaaat tcaaaaataa tgaatgttat tttattagga
     6781 tgtctagtta aatctatgca attagagtct attgattggg aaaaaattat aagtgaaaat
     6841 gttaaaccaa aatttgttga attaaacata aaagctttcc atgaaggtat ggaaatggtt
     6901 agcaaatagt ttataataga tataaataga ctatagaaat taaggtgaaa ggaagaaaat
     6961 tatgacttac agaatattag ccataaatcc aggttctact tctacaaaaa tagcagtata
     7021 tgatggagaa gaacaaattc ttgtgaagac gatagaccat ccagctgaag agattgcaaa
     7081 atataatact atacaagacc agtttgaaat gcgtaaggaa gcagttttga atattcttaa
     7141 agaaaatagt atagacttaa aatctcttag tgcaatagta ggaagaggtg gagttttacc
     7201 accagtaaaa tcaggagcat atttagtaaa tgaagaaatg attgatgtac taagacatag
     7261 accagtactt gaacacgctt ccaatttagg tgctgttgtg gcacatgcaa tatcagaacc
     7321 tcttggaatc aactcatata tttatgattc tgttgcagta gatgagctta tagatgtagc
     7381 gagaatatct ggactttgtg gaatggatag atcaagtgca gggcatgcat taaatactag
     7441 agcaatggct ttaaaatatg ctaaggataa aggaaaagat tataagagct taaacttaat
     7501 agtagctcac attggtggag gagtaagtat ttatcttcat gaaaaaggaa gaatggttga
     7561 tatgctatct gatgatgaag gaccattttc tccagaaagg tcaggaagag tacctgctac
     7621 aaaattagtg gctgcctgtt attcaggtca atattcagaa agagaaatga ctaaaaagat
     7681 aagaggtaaa ggtggtatag tttcatacct aaatactgta gatgctagag aagttgaaaa
     7741 aatgatagca gaaggaaatg aagaagcaaa aattatttat gaagcaatgg cttatcagtt
     7801 agcaaaaggt attggagagt tagcaactgt agtagatgga aaggtagatg ctataattat
     7861 aacaggtgga attgcatatt ctgaaatgtt tacttcaatg gttaaaaaga aagttgagtt
     7921 tatagcacca gtagaaatta tggcaggaga aaatgagttg gaatcacttg cttttggaac
     7981 tttaagagta ctaaatggag aagaagaagc tagaatttat agtgaaaatt aataagaaaa
     8041 actaaaaatg tttatatact aaaaaagtgg tatatccgtt ttttatacac gaaagcttag
     8101 agtataggtg aaaatgtata aaatttggat tgccactttt tataattaaa attttatctt
     8161 aacctaataa ataattctgt aaaatttcgt atttatatat atattttatt ggtatttatt
     8221 gttattatta ttatgaggag tgatttttga tgggaaaaat aagtttaaaa agtatgataa
     8281 gattattttc aggttttttt atatttgcaa tttcttctgt gcttatgatt aatgcacatg
     8341 taggtcttat gccttgggat gtacttcacc aaggtttatc tataaagcta ggcataacta
     8401 taggtcaggc ttctataatg gtaggagttg taattgttat attggatgca gtttttggtg
     8461 aaaatatagg atggggtact ttactcaaca tgacatttat aggaattttt attgatttag
     8521 taatattttc tggagtgata cctcatgcaa gcaacactta tataggtgtg tttatggttg
     8581 ttataggaat tattttggca gctatagcaa gttttctata cttaggtgtt tgtttaggaa
     8641 gtggaccgag agatgggctt atgattgctc ttcagaaaaa aactaataaa tctgtacgtc
     8701 ttgttagaac aattcttgaa atactagcat tagttgttgg atggttatta ggaggttctg
     8761 taggtattgg tacgcttgta agtgctttag gtttaggata tgtattacaa atagtgttta
     8821 gaatatttaa atttgacaca aaattgctga aacatagatt tattatagat gatataagag
     8881 aatggaagga aaagaaaagc aatgaacata aatgtaaatc aagtattgta ataaaaaatg
     8941 agcagaatta attatgctaa aatatatttg tagtttatct aatttatagt aaagtcaagg
     9001 agattttgaa atggaattat gggatttata taatgctgat ggaattaaaa caggaaatgt
     9061 tataaaaaga ggtaattcta ttgaagaagg ttattaccat ttggcagttg aagtgtggat
     9121 tttaaatagt aactctcaaa ttttaattca gaaaaggtct aaaagtaaaa aaacattgcc
     9181 aaatatgtgg ggaatgacaa ctggatgtat tgtttctggg gaagacagtt tagaaggagc
     9241 aattcgcgaa gcaaaggaag aaatagggat agatataact aaagatgaga tgaaagtttt
     9301 tagaagtatg attcatgaag atactctttg ggatgtatac ttagttaaaa aagaatatga
     9361 tatatctaaa gctattttac aggaggaaga agtaagtgat ataaagtggg taagcacaga
     9421 tgaaataaga caattactaa aagagggttt gttttttgaa tatccagaga tatacgaatt
     9481 gctatatgag atagataata ataaattaaa tatatgatgt agagcagatt tcagtataag
     9541 taagattatt taaaatgtat cttctctaaa ttttaatatt gttttatgaa aattaataag
     9601 ttatttttag tatttttcta cttgtttttt gagattaaaa taaaaagggc agtcattata
     9661 aagtcataaa aatttgggga ggatttaaac tatggaagat aacaaaggat tggcacgtga
     9721 cagttcaaaa tataaaattg tttctatatc tgtcgtagta attattctaa ttacattttg
     9781 gtatatgtta aatatggtgc ttttaacatt tattatgact tttattttct ataacttgct
     9841 tgtagccaca agaaaacgaa ttaagaagtt ttcttcttta aatattccag attcattgat
     9901 aattatagtg ttatatgcac tatttgctat attgttagta ttgattagct atgcagttgt
     9961 accaataatt atagttcaac ttacagagtt gagtagggtt tttagtgatt ttgatgtaaa
    10021 tcaatttgct caatctttag gaccgaaatt gtatcctatt gtctcaaagc tggattttaa
    10081 taaatatatt tcacaagcag ggctacttat agcatcaaca gcaacaaaag ttggaagttt
    10141 tggagtaaat atattattgg catttttact gagtttactc cttttacttg aaaaaaatga
    10201 aattaaaaat tttggagata aactttctga tagtaagatt tcttttattt ataattcgct
    10261 tgtatttttt ggaaagagct ttgttaaaaa ttttggtgaa gtaatgaaag tacaagttat
    10321 gattgccttt ataaattctg ttgtttctat gattttctta ggtttcatgg gattccctca
    10381 aatatgggct ttaggattta tgatttttgt gttgggtctt attccagtag caggagttat
    10441 agtgtctttg attccattaa ctgtaatagc atttaataca ggaggaataa caaaggtttt
    10501 tggagtttta cttatgattt gtatagtgca tgcagtagaa acatacatat taaaccctaa
    10561 gttgatgtcg aatagaacta aacttccagt ttgttttgtt tttattattt tacttgtagg
    10621 agagcattat ttaggtgtat ggggattact aattggagtt ccaatattta tgttccttat
    10681 ggatattctg ggtgttaaat ttacttctag gtaatatatt aaaataataa tttattttta
    10741 tatataaatt atgaattgtt ttaaaaaaga acctataaat agacatcatt aataaagaca
    10801 tctatttgat aggttcaatt ttgctttgta taaatactat aaaactaaat aaaaagaaat
    10861 ttatttcatt tcaaaagttt cacaacttgc atttctttca tttatatgaa ctttttcagc
    10921 tttgcaaagt tcatttttat tgtatttaca ttttacagct tcacaatgaa tgttatctgt
    10981 atcaacttgg ttagtttcat tagaacgact agttatacca gatgtatatt tgtcttcaaa
    11041 agaagcacaa taagtatttt ctgtagtagt tgcttgcatt ccatctactt ttatactacc
    11101 agcataacaa tatccactag tattataagc acaattagta gcagcacaat ttaaattatt
    11161 atttttcata aataacctcc taagttttat ttaaacttat tatgtgaagt tatctaaatt
    11221 ttatacactt atttacaatt acatttatca catttaacaa ctacatcttt ggaagtttcc
    11281 cttcctaaat ttttattaac atgatacaca tcaacatttt cagttgatcc acatatagaa
    11341 catttaaaat tgttttctag tagggttctt tctttaaata acttccaata catagaattg
    11401 atgtaatcat tataatttac cttgaggcat gtctcaagaa agtttatgta tagattgtct
    11461 aaatctcctg tatttaattc tgtatattct ggttcaaaat attcacaaca tcctctaaaa
    11521 tcgggctttg tatctgaaat aacacatcta atatcatatc tatagtctag gctaggcccc
    11581 ttaaatgaaa gatgtgtgca aaaagaacaa gatatttctg gtagattttt ttcaagttca
    11641 tgtaatttat tattcataag tattcctcca aaaaatttat tttaagtata taaatgaata
    11701 gattatttta aaacttttat ttatttatat tagagattta ggataaatat tccaataagg
    11761 agtaaatact taagtattta ttactgtatt aaataaaaaa gaggcaaatc taacttgaga
    11821 ttgcctactt ttttatcatt ttttaataaa atttattcta agtattattt tatttagatt
    11881 tagtcagcca acttagtatc tgctttgctt tttaactttc taccagagcg tacagttaaa
    11941 atagcatata atacagtaaa tatggcagct aaaacatatg atatatttat gtttaagtta
    12001 aatccaattt gagcattaaa tatataactg aatataacaa aggaataaaa cataccagga
    12061 attaagctta taatataatt cttttctttt attataaggt aaactgtaat cattgcaaat
    12121 gcaaatatgg caatagtttg attactccaa gagaagtatc tccacaatat attaaatcca
    12181 gaagcactaa gctttgcaaa tataaggatg gctatgacag gaataaatat acagattgac
    12241 aatataacac gatgtttctt ttccttttgg tcataatgaa agtaatctgc aagcattaat
    12301 cttaaagatc ttaaagctgt atctccagaa gttattggta aaacaattac acctattata
    12361 gctatgatac caccaataga accaagtaaa tctcttgcaa ccaaacctat tacatcagga
    12421 gaacctacta attctttagg tattccttta ttgtaaatac ccatagcagc tgcagcccaa
    12481 atcattgcaa ttaaaccttc taaaatcatc atattataaa aagtagtttt tccttctttt
    12541 tcatttgaaa ctgatcttgc tataagagta gcttgagttg agtggaatcc agaaacaata
    12601 ccacaagcaa ctgtaacaaa gaatgtagga ataagaggaa taccatcagg atgaatacct
    12661 ttccaatttg caagacttaa attagctaaa tcataacctt gtgtaaagat tccaattcca
    12721 acaccagcag cagataaaag tagaatagct ccaaatattg gatatacctt tcctatgatt
    12781 ttatcaattg gaaatagtgt tgcacacaag taatatagaa agataagtcc ataaactatc
    12841 cagacaactg gattatttat agtcgatttc atatttaaaa tttgagtaac aactaaatcc
    12901 ccaggtgtat aaataaaaac tgcaccaact aaaagcatta aaagacaaag gaatatatta
    12961 tagacttgaa atactttatt acctaaaaat ctactgataa gagaaggcat ttgtgctcct
    13021 ttttctctta tactaatcat tccactcata tagtcatgca aagctcctcc aaatacacag
    13081 ccgataggta ttagtatgaa tgctattgga ccaaacaata taccttggat aggtcctaga
    13141 attggtcctg taccagctat attaagcaac tcaattagac tatttttcca ttttttcatt
    13201 gggacatagt ctactccatc actttgagca agtgcaggtg tttttctgtc atctgggcca
    13261 aatacttttt cacaataagc accatataaa gcacctccta ttagtaatat agccaagccg
    13321 attaagaatg ttgtcataat atcaccccat ttataaaatt ttttggtctt tgtatacata
    13381 ttatctaaat gtgtagattt atattgaatt taattctgga caacctacaa attctataaa
    13441 gtattacaac taatataatt tcagaaataa atacatatac ataaccatgt acttagtata
    13501 caggtatttg gtaatatttt caatgttata gcaataaaga atataaacaa aattctgata
    13561 atattttaca aaaaaattaa aaaatataaa aattagttta aatagatgga gaaaaaagga
    13621 aaatcaattg agaatataaa tgtctatatc ttaacaatat taagaggttt aaaattcttg
    13681 aagaaagaat agtcaagtga tataatgggt ttacaggtgt atatacacta gtacatatag
    13741 aaagcggtgc aattatggat aaagatttaa catatcaaat caaagagcta aagaaagaaa
    13801 aaaatgcaat aatacttgcc catttttatc aaccaccaga aatacaagaa ttagcagatg
    13861 cagttggtga ctcatattat ttaagtgaga ttgctagaga ttgtaaagaa gaagtggttg
    13921 tattttgtgg agtaaggttt atgggagaaa gtgctaagat attatcacct gaaaaaactg
    13981 tattgatgcc agtaagtaac gctgggtgtg ctatggcaga tatggtagat gaagaaggcg
    14041 tgataaaatt aaagcaacag tatccaaatg ctttggttgt atgttatata aactctacag
    14101 ctaaagttaa ggcacattgt gatgtttctg ttacatcttc tagtgcaatt aaaatactag
    14161 aaaacataga taataaagaa attatattcc ttccagataa aaatttagga ggatatattg
    14221 cagaacaatt tccagataaa aactttatat tttgggacgg atattgtaaa taccataaca
    14281 atataagagc tgaagaaata attgagttaa aagataagta taaaaatgca gaagtattag
    14341 tacatccaga atgtaaaaaa gaaataagag atttaggaga ttatgtaggt agtacatcag
    14401 gaataataaa atatgctact aatagtaaaa acaaagactt tataatagca actgaagaag
    14461 gtatattaca tgaattaaag aaaaataatc ctaataaaaa cttttatatt cctggtggaa
    14521 aaatactatg tacagatatg aaaaagacaa cattagaaaa tttatatagt acacttaaaa
    14581 atatggaaaa tgaagttatt gtagaagatg aaatcatgga aaaggcttta aattcactgc
    14641 ttaacatgca caaattagca gaaggataga gggatagact tatgaattta gaacaagatg
    14701 ttttaatagt tggaagtgga gtttcaggat tatactgtgc attaaattta gataaaagtt
    14761 taaatgtgct agtagtatcg aaatcaacaa ttgaaaacaa caatacatat cttgcacaag
    14821 gtggaatttc aactgctaga aatattgatg atatagaatc ctttgtagaa gatacaatga
    14881 aggctgggca gtataaaaat agagttgaag cagttcaagt acttgcagat gaatctatag
    14941 aaaatgtagg tcagatagta gaatatggta tgcctttaga caaagaaaat ggagagatag
    15001 attacacaag agaaggagca catagtgtaa atcgaatagt tcatagtaag gataatactg
    15061 gagaagttgt ttttaaaact ttattaaaag aagctaaaac tagagaaaat ataactctta
    15121 tagaagatgc atatctttta gatatattaa aagatggcaa taaatgtata ggagctagaa
    15181 tttttaaatc taaaaaagag attcatgttt tttcaaaaat agtagtttta gctacaggtg
    15241 gaattggtgg gctttttaaa aattctacta atcaaagaca tcttacaggt gatggaattg
    15301 caatagcttt aagaaataat ataaaaatag aaaatttaga atatattcaa atacatccta
    15361 cagcctttta tgaagaaaat aatgaaggta ggagaatgct tatatctgaa tcattaagag
    15421 gagagggagc taaactttta aataaaaata aagaaagatt tgtggatgaa cttcttccta
    15481 gagatgtagt ttcaaaagct atttttgagc aaatggaaaa agataaatta ccttatgtgt
    15541 atttagatgc aacacattta gatagtgaat atttaattaa tagattttct tttatttata
    15601 atgaatgttt agctagagga acagacataa cgaaagaatg tataaaggta tctcctgcac
    15661 aacattattt tatgggcggg atacatgtag atttggattc aaaaacatct atggaaaatt
    15721 tatatgcagt tggtgaaatt agttgtacag gtgttcatgg agccaataga ttggcaagta
    15781 attcattact tgaagggctt gtattttcaa aaagagcagc taaaaatata aactcagtta
    15841 tagataatgt aaaagttaag tttatagatg taccagatat ggatatagat atagaacagg
    15901 taaaaaaaga aaataaaata ttagtcataa aagaaattga aagaacaagt gaggattttg
    15961 gcgatgaatt atttgattat tgatagaatg atacaggatg ctttgataga agatgttcca
    16021 agtgaagata taacaactaa ttctattgtg gatgaaaatt ctaaatctac tgtagattta
    16081 atttgcaaac aagatggtat aatagctggc ttaggtgtat ttaagagagt ttttgagata
    16141 cttggtgatg tagatgtaaa attatataaa aatgatggtg atagagtaaa gaatcgtgaa
    16201 aagatagctg tattaacagg tagtactaga aacttacttg taggagagcg tgtagcattg
    16261 aattatttac agagaatgag tggtatagct actattacaa gtaggtatgt tgaaaagctt
    16321 gaaggtacaa atacaaaatt attagatagt agaaaaacga tacctaatct tagaatatta
    16381 gataagtatt ctgtcaaagt aggtggagga tgcaatcata gatttaattt atctgatgga
    16441 atattgctta aagacaacca tattggggct gctggtagtg ttaagaaggc agttgaatta
    16501 gctagaaaaa atacttcttt tgttagaaag atagaagtgg aagtggaaac tttagacatg
    16561 gtaaaagaag caatagaagc caatgcagat ataataatgt tggataatat gagtttggaa
    16621 atggcaaaac aagcagtaga tattataaat ggaagagcca ttgtagaatt ttccggaaat
    16681 gtaaatttag atactataga agatatagga aaaattggtg tggatgttgt ttctgtagga
    16741 gcgcttacac attctgttaa agctctcgat ataagtatga agaatttgag aaatatataa
    16801 attttatatt gaaataaact taattaaaat agactcctta aatttgtttt ataaatttaa
    16861 ggagtctatt ttttattcat taaaattaat aaaaatagaa aaaaatcaat caataattta
    16921 ataaaaatag ataaatacta tatacacttt taataatata gttaatatct attttacaga
    16981 taaataagaa taaacttata atataattgt atctgtaaaa ttttgtaaaa tataaaaagt
    17041 atagggggaa taaaaatgaa agtagcttat atttttagta gtactaatac acataaaatt
    17101 ctagacaaaa tgataatacc acaacttgaa caaggaactc atggagtaga cgttctaggg
    17161 atgtttttct ttatggataa taccttattt ttatctaaag gtaatcctgt aggcgagaga
    17221 ctaagtaaat tacatgaaaa aactgatatg ataataatgg cttgtgacca atgtgctata
    17281 gaaagaggta tagagaataa tttggttgat ggagctacaa ttggatgttt cccaaaccta
    17341 tatgcttgtt taggcagtgc aggtgtggac caagttataa cgttataaat tagtatagga
    17401 tacaatccat agaaaaattt attttctgtg gattatttta agtaaaagat aaattgttta
    17461 aagtagggga tgaacaaatg tctaaaattt tagttgaatt tataaacact tgaccatctt
    17521 gtgatgaaca tggagtgttt ataaaaaaaa tgtcagaaaa gtatgctgat aaattggaaa
    17581 taaaattata tcaagcaggg aaagattttt cgtatataaa aaaatatggg attataacaa
    17641 aaggaacttt aataataaat cagaaaaaga aatatgatag attaaataaa gatacaattg
    17701 aaagggcaat agtagaagct ataaacaata attaaaagtt taaaaattag gagtatcaaa
    17761 aatgaatata ttagaaattg ttttaaatgg agttaaagat actatagtag cttctatagt
    17821 tacactgaat ggagcatcag gatggcttat gataagtttt atcttggcag gtgtacttca
    17881 caatgtttta actccaagaa ggtttcaaaa acaacttgga aataagaaat tttcttcttt
    17941 aattaaaagt actatctcag gaatgttttt accaatatgt agttgtggag taataccact
    18001 tggaataagt atgtattatt caggagcata tttaggacca gtacttgctt ttatgacatc
    18061 aactccaata ataaatccaa tagctgtagt tctttgtcta ggtcttttag gtcctaaagt
    18121 tacaatcatt tatctaatag ctggtttttt agtaccattt ttagtaggat ttttaggaaa
    18181 ttgttttgga aaagaagaat tatcacttca taatgattca gaggaagaag taatagaatt
    18241 agaagaagaa aaaagaagtt ttatggaaaa tatgattgat ggtcttaaat ggtcttttgg
    18301 tgaattagcc cttactatta gtaaatatgt agtaatgggt atggtttttg caggatttat
    18361 aacaactatt tttccaaata gtataattca aaaatattta ggaaatccag gtttgctatc
    18421 tcttggaagt atagcaatac ttgcatgtgt tatgtatgta tgtgctgtag gacatatacc
    18481 gtttatagca gcccttgttg caagtggagc atctcctggt atagcgataa catttctaat
    18541 agctggagca gctacaaatc taccagaact tataagtatg tttaaactta ttggaaaaag
    18601 aactgttatt atatattctg taactttgac actgtcttca ataataattg gatatataac
    18661 aaatttactt ttagtgaata ataactcagt aaatttagat gcaacaaatt cttcaataca
    18721 gttggcaaat aaactaatgt taaatatacc tgatagtttt gagtatgtat gtactatttt
    18781 aattatttta tttgcattta aatcaataat accaaagatg aaagaggtca taggaaatca
    18841 tgcttaaaaa gatagttata atatcctgca tagtagttgt gttaatcatt ttatcaaaaa
    18901 tagttgatga taacatcaaa gaagatgctt ctattccaaa tgtgaataaa gaaacattag
    18961 aatattttag aaaaaattat aaagaagaca taataacttg tgctgaagaa gacttaaata
    19021 atgatgggaa aaaagattta gtagttatat ataaaaaaag caataattca aatgaaatgg
    19081 tagtcgtagt ttctgataaa aatagccact atataacaaa gccaatacca gcacctatag
    19141 agaatcaaac tataaccttt aaaaatattg atgataaagc accaatagaa gtaattgtat
    19201 cagggtctaa aaatggtaat gtaggatatg ctatttacag agtagagggt aaaaaatttg
    19261 ttgatttatt tggcgaagat atggacaaat gttgctaaaa ataaaaataa tacataaagg
    19321 tgggaaattt atatgaaatt atctaagaag attcaagcat taatcttatt tggtgtaatg
    19381 ggtacatcta tattaacagg atgtagtagt aaaccaaaag aaaaagaaga agcaaaaact
    19441 ggaaaagatg attatacaat aacagtaggt tattataatt gtgaccatat gactgcggga
    19501 ccagtagcag aatcggcagg aatatataaa gatttaggtc ttaatgtaaa aactgttgga
    19561 aatggtaaag ttcctgaagc tatggcagca ggaaaaatgg atgcaggtta cataggaact
    19621 aaggggttag ttggagcaat acctaagggt tcaccaataa caatagcagc taacaaccat
    19681 actggtggtt ctgaatattt aatagtttca aaagatataa aagaacctaa agatttaata
    19741 ggtaaaaaaa ttgcaacaga tatgagtgat ttcttatgga caagtgatta tggtccagaa
    19801 acaggtctac caactgaccc atcaaaatat gaagttgtaa atatggattc agacaaagat
    19861 aaatatttag ctcttaaaac tgggaaaatt caagctttta catcttgtga cccttggggt
    19921 tctgtagctg aaaatgacgg tgcaggaaaa ataatagcat ctactcaata taaagaaaaa
    19981 gctaatggca aagaatataa ctgttgttca ttctcattaa acaaaaactt tataaaagaa
    20041 catccagatt tagcaaaaaa attggttcta gcacatacaa aagcaataga atatatatat
    20101 acaaatccag ctgaagcagc taaaatattt gcaaaatatt ataatgtaga agaagaagtt
    20161 gcacttagaa ctatctataa gaagactgtt ggagaaggta gaactcttac ttggaaagtt
    20221 acaggtgaag aatataaaaa taatttacaa atgtataaag atttaaaggc tttagatgat
    20281 atacctaaat atgaagatac gattgataca agcttattgg actcttgtgg agctgatgat
    20341 tttgacaaat ttataaaaga aaaagttaac ccagagtttc ctgaaggtat gagttatgaa
    20401 gactggaaag caaaggtatt ataaataaat ggagaatatg aaaaatataa taatactttt
    20461 tatcattatt ttgggaagta tatttataat attttttaat tttgaagata aagaagaaat
    20521 aaaaggaaaa ttaagaatag gggtatctga tgatacctct ggttttgtaa taaattatat
    20581 ggtaaataga gactactttg aaaatataaa agtaagtgat ataatggata tttttacaat
    20641 taatgattgc tgagcaagta caatgcagtg ggctctgagt tcagaggaaa tagatatagc
    20701 aatcatatgt aaagatgctg caaaacaata tgtaaatgta aatagtggat ttgaaattgt
    20761 tggtactgta gttcaaaatt ctgatatatt tctattaagt gataataatc ctgaaaagat
    20821 aggagtcatt caaaatagag attaccagtc agaattggtt aaaaaatact acaagaatgt
    20881 ggaagtagct cccttattag ggactgcact agcatatgga ttagaaagta atttagtaga
    20941 tggtgttgta atagattgca taaagtcaat tgggttagag ggcaaaagaa aatctacaac
    21001 taaattagga gattatgata cctatgtatt agttgtaagt aaagagttta aaaaaagtaa
    21061 agcatatagt gattttataa cactttacaa taaaagtgtt gatgctttaa agaataaagc
    21121 tacatttaag aaagcattaa aagagtacag gaatataaga aatatagata tggaggaaat
    21181 aagcaattgg aaattaaaat ttctaaagat aaaagcgata gattaactaa gaaaaaaact
    21241 gatggtagta tggatatttt atataaaata atactattag ttattatatt tttagtctgg
    21301 cattttgcag caaaagatat agggagttct ttgttacttc caatgccagt agatgtaata
    21361 aaaggtttct ttttctgtgt gacagatgca gagacagtca ctaatctttt tataacattg
    21421 cagagagtac ttaaaggttt tatgtatgca ttattgtttg ggcttccaat aggatttatt
    21481 atgggatttt ctaaaacttt tgaaagggtt ttatctccag tagtagattc tgtaaggcaa
    21541 gttccaataa tggcatgggt tcctcttact atagtatggt ttggtatagg agatggtcca
    21601 acaatatttt taatagcttt ttcaggggtt ttccctataa ttttaaatac tattcaagga
    21661 gtaagagcaa tatcaaagga ttattataat gcagctagaa gtatgggagc atcgccaatt
    21721 gtgatattta caaatgtaat tgttcccgca tctcttccag atatactaac tggttcaagg
    21781 attgctataa gtacaggatg gatgtctgtt atctgagccg agtttatagc tacgagtgcc
    21841 ggtctcggtt actcaatggt acaagctcaa acattaatgc atacagatgt attaattggg
    21901 cttatgatat ttgcagcttt aataggattt ataatagata gggtattaaa gtttataaat
    21961 aaaatccttt gtagatggag gtttgcagat taatgaagtt aagtgtaaga gatataaata
    22021 agacatttgt aaataataga gtacatacaa aggttttaga agatataagt atggatatag
    22081 atgatggaca atttgtctgt cttttaggac catcaggttg tggaaaaaca actttactta
    22141 ctataatagg tgggtttcaa aaatcagaac gtggagatgt ttttataaat gataaaagag
    22201 ttaaaaaacc gggcatagat agagctttta tatttcaaaa ttatgcttta tttccttgga
    22261 aaactataag aggtaatgtt ctatttccta tgaaacagca gaaaatacca aaagaaaaaa
    22321 gagaagaaat gctagaagaa cttttggtta tgtcagactt gaaaggaaaa gaaaaactgt
    22381 ttccgcatca attatctgga ggaatgaaac aaagagtggc catgataaga gctttagcat
    22441 gtaatccaga ggtattacta atggatgaac cattaggagc tgtagatttt caaatgagac
    22501 aaaatttaca ggaagagcta gaacgaatat ggataaagaa gaaaataact gcattaatgg
    22561 taactcatga tgttgatgaa gctgtatata tgagtgatag agttatagta atgtcgagag
    22621 ataaaggaag aattattgat gatataaata tagatattcc aagacctaga attagaggaa
    22681 gccaaaaata tgaagagtac aaaaacaaat taactgatac tttatcaaag tgttatgaag
    22741 tttaaagagg agtagacatg agtcaaatag aaaaatacaa tagtttggat aatacacaac
    22801 agaaaataag aatctctatt atggatatga taatagataa aggttctagt gttacacttc
    22861 aagaagtgac tgaatatata agtaaaaagt tgaatataga aaaagaatat atagaaagga
    22921 cactacaata ttttatttat aaaaatataa tggttgttga tgggaatagt ataaatttta
    22981 tatatcctgt atcagcattg ccaacaaatc ataaagtaac tttgagagat aatagaagtt
    23041 tcagtgcaat gtgtgctata gatgctatag gaacctcttg cacattcaac caagatataa
    23101 aaataaactc tatttgtagt gctacaggta aggaaataga agtaattata aaaaatgaaa
    23161 agattgagta tgtgaataat ccaaacttaa gagtgcttca tataaatctt gataagcatc
    23221 taaattgggc tgctagttgc tgaaatataa tgaatttctt ctggacgaag agtgattttg
    23281 atgcttggac aaacgaagcg ggattatcta atgatgaaga tatatattgt ttagatatta
    23341 atgaggcaat agtagaatca tataaaatat ttaaactcaa acaaaaggta ctatcataga
    23401 tatacatata tgaaaaacta aaaatgacct ttagataaaa atgataggta atatccatac
    23461 tatataaatt agtatagagt agggatgtga aaagtattta aatctaaagg tatattttta
    23521 gtttttttta gatgattaga tagccttatt aagtatatat ttatttataa cctttgcaac
    23581 tccattttca ttattagtat cagtaacata atctgcaatt tcctttatac tattacgtgc
    23641 attccccata gccacaccaa gacctgcata ctttatcata tgttggtcat tttcttcatc
    23701 gccaacagca atagcatttt ctataggtat atttagattc gtacataatg ttttaagagc
    23761 agttccttta ttagtagttt tacctaaaaa ttctagataa aaaggagcag accttactat
    23821 agtatattta ttgaataaat ttttaggtag ttgctgtatg atttcttcca atctttctgg
    23881 ttcatcaact atcataattt tacagatttt aatagattca tcaattttat ctttttgtat
    23941 atatttgact tctatattat ttaatttcgc ttcatgaagt gtatatttgc caggcgtttc
    24001 atttggtgta attaaatttg tcattgtatt tatgtgtatt ttagtcttat attttttaca
    24061 aacgttatta tatataaaat caaaatcatc taaactcatt tctacatcat gtatgatttt
    24121 ctcactttta acttcttgaa taattgctcc attaaagcat ataacataat caccttcatc
    24181 tgttaaattt agttgattta gaagttcttt tacaccagga agaggtctgc ctgttgttat
    24241 aactactttt gccccagccc tttttgcctc ctgaatggac tcaaagactt caggagtaat
    24301 ccttttttgt gtatttaata ttgttccatc aatgtctagt gcgattaatt tgtacatatc
    24361 aacctccaat aaattatata ctcaaatttc ctgtgaaaac gttgacacag ctagtgttga
    24421 acaaagtttc agtgggaaat atggtttgtt ttgtatacta ttatatcaag agataagtaa
    24481 tttagaaagt ttaataatat taattttttg ataaaaatat tacttatgtg agatgagaat
    24541 gtaaaaactt tgtaatcaat aattccaaaa ttcttaatta caaataatgg tatcgtttgc
    24601 attgttaata taaataaatg tatattatat agttagaagt ttgaataaat agactgatgg
    24661 taatttgtta tattagttag ttttgtgtta taaaatattg taattccatt aatcatatgt
    24721 tattattcaa catgattata atataaagaa tatttgatat atataataat ttataaaaga
    24781 gtattacata gtacatgtaa atctatattt ataaatacaa taaagtaaag ttgtaaagga
    24841 gtgctttaaa tgaagttttc agagtttaag tatgaaagac ccaattataa tagtatgaaa
    24901 aaagagtttt tgagttgtgt agaagatata aataattctc gtaattataa agagcaacaa
    24961 aaaaatatac ataagattaa tttattaaga aataaaatag aaacgttgtc aaatatagct
    25021 tcgataagat atagtacaga cacatttaat aaattctaca aagaagaaaa aaattattgg
    25081 gatgaatata tgcctttgta tgaagaatta aattcatatt tttataatgc cattgtaaat
    25141 tcaaaattta aatatgattt gataaaagag tttggagagc aatttttcac aatagtagag
    25201 tattctttaa aaagtttttc caaagaaata ataagtgaat tgcaggaaga aaataaattg
    25261 tgttcagagt atactagact tttagcttca gctgagataa tgtttgatgg gaaaataaga
    25321 aatttatctg gtatggggaa attcatgtat tctaagaata gaaaaacaag agaattagca
    25381 aacaaagcat attataattt ctttgaagaa aatgaaacta aatttgatga catatttgat
    25441 aaattggtta aacttagaga taagatgtct aaaaaattag gttttgaaga ttttgtagag
    25501 cttgggtatg ttagaatgat gagaagtgac tacagagaat atatgataaa aaatgttaga
    25561 aaacaagtac taaaatatgt tgttccaatg gcaaatgagc tttatgaaaa acaagctaaa
    25621 agaattggtc tagaatattt aagttacata gatgaaggcg tggaattttt aacaggaaat
    25681 gcaagtttaa agggagattc aagatacata attaaaaatg gtaaaaggat gtattctgaa
    25741 ctgtcaaaag aaacaaatga attttttgat tttatgttag aaaatgaact tatggattta
    25801 gaaacgaaga aaggaaaagg agctggaggg tattgtactt atataccaga ttataagtca
    25861 ccattcattt tttctaattt taatcaaact gctgatgata tagatgtatt aacacatgag
    25921 gcaggacatg catttcagtt gtatatgtct agatggattg atatgccaga aattaatttt
    25981 ccaacgcttg atagttgtga aatccattca atgagtatgg aatttataac atggccgtgg
    26041 atggatttat tttttaaaga agatacagat aagtacaaat tcactcactt atcatcttca
    26101 attaaattta taccttatgg agtaattgtt gatgaatttc aacactatat ttacaaaaat
    26161 cctaatgtag ataagtcaaa acgaaaagaa atctggagat ttttagagaa aaaatactta
    26221 cctcatagaa aatatggtga taattctttt ttagagagag gttgttggtg gtttaaacaa
    26281 ggacatatat ttaaaaatcc gttttattat atagattatg tattagctca aatttgtgcg
    26341 ttacaatttt ggaaaaaaat gattcaagat agagatgcag gatggaaaga ttatataaat
    26401 atctgtaaag ttggaggaac aaaatcattt ttagatattg tgagtatggg aaatttgtat
    26461 tctccatttg atgatggctg tattgagtct attataggtg atgttaaatc ttggtttgat
    26521 gaaataaatg acagcaaatt ataaaaaatt atgagaattt tattgtataa aagtgaaaaa
    26581 ttaaaaaata tataaaaagt gattatttgt tgtgaaaata gtcttgtttt taactaaatt
    26641 gataaaataa actaaaaaag atttgtactt aaaaaaaaaa gaaaatacaa gaaatatgca
    26701 atatcaacat ttggaaaaaa aagtaaaaat gtatacaaaa tacatataaa aaaactattg
    26761 caaattttat agaatgttta tatacttaag tcaacagaga tagaaaaaag ctatttatag
    26821 aaaacaaagg ataaattgct taattttaaa ttttctgaaa aaaataataa tccggatgaa
    26881 gagtatggga gactgcttta taaaaagcga ccgaagggac aagtttaatc ttacttaccc
    26941 aaaagattag atgaaattct caggtaaagg tgactgtact tggacggggc tctgaaaatg
    27001 attttaacag ggaagacaaa ttgggagatt tgtttttcct gttttttatt tatcaagtaa
    27061 taaataaaag tttgaaagag agtaaaaaaa tgataataat tacgaataat ccaaaagtaa
    27121 aagaagaagt tcaaggcaga gaagttttgt ttaaagacac aacttatatt ggaatcttag
    27181 aagcaagtag agatttgata catgaaggtt atgaactttt atctcatcca ctctatggga
    27241 gtgtaaagcc aaatgaaaca ccttacagga ctgttatact aaagaaagga aatcgtttgg
    27301 atataaactc tttaacctta attgaagaag caatcatcac tgcaagtaaa tttcagaata
    27361 ataaaaagac tccaaaatgg acagaaagtg ttcaagatga ttttagagta attgattatg
    27421 atatatttta taatacaata caaagaatgc agtatgaata aaaaaaaatt gttaaaaagt
    27481 tattaaaaca gcctaaatat taaatctaat ttaatattaa ataaaatgag aaatttaatt
    27541 ggggaggata agtcaaaatg gaaaatgtgt atgacttagt aataattggt tcaggaccag
    27601 cagggcttgc agcaggtctt tatggagcaa gagcaaagtt aaaaactcta atcctagaaa
    27661 aagacaagac tggtggacaa attgtaataa cacatgaaat tgcaaattat ccagggtcag
    27721 ttccaaatgc aacaggacca agtttaatag ctagaatggt tgaacagtgt aaagagtttg
    27781 gagcagaaat gctaagagac aacattgttg atactgagct agatggagat ataaaagttt
    27841 taaaagggga aaaggcagaa tatagagcaa aagcagtaat aataggaaca ggagcaacac
    27901 caagaaagat tggttgtcct ggagaaaaag aacttactgg taaaggggtt tcatattgtg
    27961 caacttgtga tgcagatttc tttgaagatt ttgaagtatt tgttgtaggt ggaggagaca
    28021 gtgcattaga agaagcaatg tatttaacta aatttgctag aaaagtaaca attgtacata
    28081 gaagacaagg atttagatgt gctaagagtg ttgaagaaaa agcaaaagct aatccaaaga
    28141 tagagttttt attagataca gtaattgaag aaatcaaagg tgatggaata ttagaatcag
    28201 ttgtatttaa aaataaagtt actggagaaa ctcatgaata ctttgcagat gaagaagatg
    28261 gaacaatggg agtatttgtg ttcgttggat tagatgctca aactgattta ttcaaaggta
    28321 aagttgatat ggatgaaaaa ggttacataa taactgatga agatatgaga actaacatac
    28381 caggcgtatt tgcagcagga gattgtagat ctaagactct aagacaggtt gtaactgcaa
    28441 ctaatgatgg agcaattgca tctatagtag ctgagaaata cattgatgaa aaatttggga
    28501 actagaatct gtaaattaag aaatagtgtc tgtaaattta atgaataata tttaaaatta
    28561 aaattaatat ttaaaataaa aataaaaatt tcaaggggga ataagaaaat gttagattta
    28621 gataaggcta catttgaaga agaagtttta aatgcagaag gttttgtatt tgttgatttt
    28681 tggagtgaag gttgtgagcc ttgtaaagct ttaatgccag atgttcataa attagctgag
    28741 acttatgggg ataaaataaa attctgtaag atggatacaa caaaagctag aagattagct
    28801 ataaaacaaa aagtattagg tcttccaact atggctatat acaaagatgg agaaaaagta
    28861 gacgaagtta ctaaggatga tgctacagtt cctaatatag aaaacatgat taaaaaatat
    28921 ctttaatatg ttgataaatt attaacat
//
LOCUS       DAELNY010000034        27970 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.034, whole genome shotgun sequence.
ACCESSION   DAELNY010000034 DAELNY010000000
VERSION     DAELNY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.034"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..331
                     /locus_tag="KPE55_001266"
     CDS             <1..331
                     /locus_tag="KPE55_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492359.1"
                     /translation="FFTTLLSIKTKLSTAMNITTLLVIIYLIATYINSPNLKFNSSYS
                     PEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINFGNPLGKILYIAIALLLIFSLFS
                     LIKSLLL"
     gene            451..927
                     /locus_tag="KPE55_001267"
     CDS             451..927
                     /locus_tag="KPE55_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0492360.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            1313..1780
                     /locus_tag="KPE55_001268"
     CDS             1313..1780
                     /locus_tag="KPE55_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0492361.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(1840..2412)
                     /locus_tag="KPE55_001269"
     CDS             complement(1840..2412)
                     /locus_tag="KPE55_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0492362.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(2475..3572)
                     /locus_tag="KPE55_001270"
     CDS             complement(2475..3572)
                     /locus_tag="KPE55_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0492363.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(3586..4275)
                     /locus_tag="KPE55_001271"
     CDS             complement(3586..4275)
                     /locus_tag="KPE55_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0492364.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(4440..5606)
                     /gene="rpoD"
                     /locus_tag="KPE55_001272"
     CDS             complement(4440..5606)
                     /gene="rpoD"
                     /locus_tag="KPE55_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG0492365.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(5624..7414)
                     /locus_tag="KPE55_001273"
     CDS             complement(5624..7414)
                     /locus_tag="KPE55_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG0492366.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(7669..8454)
                     /gene="aroF"
                     /locus_tag="KPE55_001274"
     CDS             complement(7669..8454)
                     /gene="aroF"
                     /locus_tag="KPE55_001274"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0492367.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(8435..8941)
                     /gene="folK"
                     /locus_tag="KPE55_001275"
     CDS             complement(8435..8941)
                     /gene="folK"
                     /locus_tag="KPE55_001275"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG0492368.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(8934..9299)
                     /gene="folB"
                     /locus_tag="KPE55_001276"
     CDS             complement(8934..9299)
                     /gene="folB"
                     /locus_tag="KPE55_001276"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG0492369.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(9310..10101)
                     /gene="folP"
                     /locus_tag="KPE55_001277"
     CDS             complement(9310..10101)
                     /gene="folP"
                     /locus_tag="KPE55_001277"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0492370.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(10165..10737)
                     /gene="folE"
                     /locus_tag="KPE55_001278"
     CDS             complement(10165..10737)
                     /gene="folE"
                     /locus_tag="KPE55_001278"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG0492371.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(10763..11464)
                     /locus_tag="KPE55_001279"
     CDS             complement(10763..11464)
                     /locus_tag="KPE55_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492372.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(11498..12241)
                     /locus_tag="KPE55_001280"
     CDS             complement(11498..12241)
                     /locus_tag="KPE55_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG0492373.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(12231..13580)
                     /gene="pabB"
                     /locus_tag="KPE55_001281"
     CDS             complement(12231..13580)
                     /gene="pabB"
                     /locus_tag="KPE55_001281"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG0492374.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(13582..14166)
                     /locus_tag="KPE55_001282"
     CDS             complement(13582..14166)
                     /locus_tag="KPE55_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG0492375.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(14190..14909)
                     /locus_tag="KPE55_001283"
     CDS             complement(14190..14909)
                     /locus_tag="KPE55_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0492376.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(14937..16001)
                     /locus_tag="KPE55_001284"
     CDS             complement(14937..16001)
                     /locus_tag="KPE55_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0492377.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(16145..16954)
                     /locus_tag="KPE55_001285"
     CDS             complement(16145..16954)
                     /locus_tag="KPE55_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492378.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(17196..18062)
                     /locus_tag="KPE55_001286"
     CDS             complement(17196..18062)
                     /locus_tag="KPE55_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0492379.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(18582..19271)
                     /locus_tag="KPE55_001287"
     CDS             complement(18582..19271)
                     /locus_tag="KPE55_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG0492380.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(19264..19635)
                     /locus_tag="KPE55_001288"
     CDS             complement(19264..19635)
                     /locus_tag="KPE55_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG0492381.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(19817..21163)
                     /locus_tag="KPE55_001289"
     CDS             complement(19817..21163)
                     /locus_tag="KPE55_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0492382.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(21345..21938)
                     /locus_tag="KPE55_001290"
     CDS             complement(21345..21938)
                     /locus_tag="KPE55_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG0492383.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(22003..23472)
                     /locus_tag="KPE55_001291"
     CDS             complement(22003..23472)
                     /locus_tag="KPE55_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0492384.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(23587..24978)
                     /locus_tag="KPE55_001292"
     CDS             complement(23587..24978)
                     /locus_tag="KPE55_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG0492385.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(25144..25392)
                     /locus_tag="KPE55_001293"
     CDS             complement(25144..25392)
                     /locus_tag="KPE55_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492386.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            25658..27787
                     /locus_tag="KPE55_001294"
     CDS             25658..27787
                     /locus_tag="KPE55_001294"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG0492387.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
BASE COUNT         8786 a         4937 c         2901 g        11346 t
ORIGIN      
        1 gttttttact actcttttat caataaaaac aaaactatca actgctatga atattacaac
       61 gcttttagtt ataatttatt taatagcaac atatattaat tccccaaatt taaaattcaa
      121 ctcatcttat tctccagaag aagatgagaa gtattggata gcaggcatca tgtataataa
      181 tccaaatgac ccatcattaa tggttaataa aaggtttggt attggatgga ctataaattt
      241 tggcaatcct cttggaaaaa tactatatat tgctatagct ttacttttaa ttttttcatt
      301 gtttagccta ataaaatctc tcttacttta aaataccata aaaggtatct tacttgtatt
      361 attgggtatt atattttgta ggataagaaa tatttcatat tatcgttgaa gtatagtatt
      421 tatatattac taagaatgga gattttcata atgttgtcaa aagctgaaat caaatatttt
      481 aatgaatgtg cttctgagat actttcttct gaaaaagttc aacttatgag aacatttcct
      541 catcatggca atgtttcatg tcttgaacat agtttgtcag tagcatatta tagttatcta
      601 ttatgtaaaa aattgcactt aagtgttgat attcaaagtg ttattagagg agctttacta
      661 cacgactttt ttctttatga ttggcattac aaaggtgata gaaaaggatt acatggattt
      721 actcacccta gagaagcatt aaaaaatgca actttatttt ttcaaataaa tgaaaaagaa
      781 acggacataa ttttaaaaca catgtggcca cttactgtta agccacctag atacaaagaa
      841 gcatttattg tatgtttgtt ggataaattt tgctgtttag ttgagacttt aaagatacat
      901 tctttattat ctccttacca tgtataggac aattcccaca acacttttat aacttaattt
      961 tgaagaggta ttaagtatta tattcaactt gatacctctt tactatttat tattttatat
     1021 aataatattt tctttataat atagttataa ataacaactt ctaccctcga taacttcccc
     1081 ttcttataga catgcttact atcttaaata acaagaaacc agctattaaa tttattagta
     1141 ttgtttatac tactctaaca ataatatcat aaataaacct ccaaatagct tctctttcac
     1201 taccttttat cttaaactat atttttttct taaaatttgt aaaaaacata gcataatctt
     1261 tataaatgtt ctaaaattta atttaagcat caaaattaga aaggatgata ttatgtttaa
     1321 agaaatgaga ttgaaaaaaa gagaaatgac aaaagaagat actgttgaag tattaaaaaa
     1381 tggtgaattt ggtacttttt ccactatatc tgaaaatggt tatccttatg gagttgctgt
     1441 aaattatgta tactttaatg attctattta ctttcattgt gcaagaaatg ggcataagtt
     1501 agataatata tcgaaaaaca acaaagtttc ttttttagta gttgctaatg aaagtgttat
     1561 tccagataaa tttagtacta cttattctag tgctatagtt tttggaaaag cttgcactgt
     1621 agaaaatgaa gaaaagaaaa atgctcttgt agaaataata aaaaaatatt ctaaaggatt
     1681 ctttgaagaa ggcatgaagt acatagagaa ggatatgaac ttaacaactg ttgttaaaat
     1741 agaaattgac catatttctg gtaaagcttc acgtttataa ttaagtaaaa atacctaaaa
     1801 gtatatacag tcgcttttag gtatttttat atgatattct tatatgttaa gtctttttag
     1861 gtaatctctt atacctatct tggcatcatc atttggtctt atatcagaat tatctcctgc
     1921 tatatgaagt attccatacg ctggataacc tcctcttact gaacatggaa ctgacaaaag
     1981 agtcattccc atatgttggg caaatatatt catagcttgc aaagctaatt ctcctcctcc
     2041 atgttgtgaa cctgctgtag caaaacaacc aaatatttta ccactcaaag gagtacctac
     2101 ccaaatatca gaaaaactat ccataaacat cttcattggt ccagatacat ttccataata
     2161 tgttggtgaa cccataaata tagcatcata atctaatatt tcttttccag atttaattac
     2221 atttatactt tcaaattcgt ctttgtattc tttggaattt atcaaataat attgaggaag
     2281 tgtttttgca acttcgtcac ttactctaaa tatatctacc tcgatattca tttcttcaag
     2341 cgcttcttta tattctctag ctaataaata tgtactccca caaacactat gaaaaattac
     2401 tgctactttc atcattatcc ccctaattaa actcaaattt attttttata tagtattttt
     2461 attatcaaac actgctaaat agtactaaat ggattcaaat atacattact ttttattact
     2521 tcaatttcac taaattcatc taaaaatctc ttcataacat ttttgaatat atcttctgta
     2581 tcaaaatgac cacaatccac tatacacatt ccaatatcaa gtgcatcttg tgcctcatga
     2641 tactttacat cacccgtaat tagtacttct gcaccttgtc tcttagcttt ttttacaaat
     2701 tctgaaccag ctcctgttac aacggctact ttcgttatgt ctgtactaag gtttccaact
     2761 actcttatgt gtttcatatt taatttctct tttattttcc ttgaaagact ttctaatgtc
     2821 atacttttat caagtttact tattctacct agtccaaata tctcaccttt attttctaac
     2881 ttgtacaaat catatgcaac ttcttcataa ggatgtgcat ctaacataga acttattacc
     2941 ccacctaaaa gcttctgtgg tactacagtt tcaattttaa cttcatttac agtttctata
     3001 tcatttacac ttccaagaaa cggattagag ccttctaatg gcttaaattg tccttcacct
     3061 tctattgaaa acgtacaatc actataattc cctatatgac ctgctcctga attacttaaa
     3121 acctttctaa gttcatctga atagttatgt ggaacgtata ctgctaattt atacaaattt
     3181 tcgcttttag ttatatctaa aacctttgaa ttaccaaatt ccataatttc cataaaatag
     3241 tcatttagac catcaaatgc tatatcaaaa ttcgtatgca tagaataaag tgatatatca
     3301 tttttaataa gtttttggat aagtctgcct tttaaatctc cactattaat tttattaatt
     3361 ttcccaaata taaaaggatg atgtgtcaca attaaatcta tatttttgga tatagcttca
     3421 tcaataacat cttcattagc ttctaaagat accaatacct tttttacatc catatcaaaa
     3481 tctccaacta taagacctac attatcccaa tcctctgcta agtttagagg atactttttt
     3541 tcaatttttc tagttagact ctttaatagc atatatacct cctatttatt ttattaaatt
     3601 ttcaagtttt tttattgcaa cttcgctttc ctttctcttc ttatctatag cttccccatt
     3661 tttcccttct aatttattaa ctatagaact atatgtcttt atcttttttt ctataaaatc
     3721 attaaaaaga gaatctttgt tttctaaaag ctttattcca acctcataat atatctcatc
     3781 ttctattata gtatttttac ctgtatattt ggcaacaatt atctcatata ttctaaaatc
     3841 ttctctcact aaaacttctt ctaaaatttc atatccgttg tttaaaaggt aatatctaag
     3901 ttcttcttgt gcttgcattg gctgtaatat taatttttct acattatgtg ctacttcttt
     3961 tttagcctcg agaagctcac ttatcaagat tccacccata ccagcaatta tcacttcttc
     4021 tacttcccct atttcaagta tctctatacc agaacccaat cgtaaatcaa ctttatctaa
     4081 aagattgttc tgtatgactt ctttatgtgc attgtcaaga ggtcctttat taacatccgc
     4141 tagcactgca aatggtactt ttccctcttt taacaaatat actggtatgt atccgtggtc
     4201 cgtccctata tcagctattt ttttaccatc tgacactaat gatgctattt tcaataatct
     4261 atctgttaat ttcaaatata attctccttt caattccctt tatttatagg atttaatcca
     4321 agttatagat attagactga aataactaca aaaataaaat taaatcttta ctacaaaata
     4381 gccatgagat atcacttatc tcatggcatt tatcacaaaa acatcaatga tgtatctttt
     4441 tagtctaaat aatctctaag tttttttgat ctacttggat gtcttagttt tcttagtgct
     4501 tttgcttcta tctgtcttat tctctctcta gttacatcaa actcttttcc aacttcctca
     4561 agagttctgg ctctaccatc ctcaagacca aatctaagct ttaatacttt ttgttctcta
     4621 tcatttaatg aaccaagtac atcttctatt tgttctttta ataaagaata tgctgctgcc
     4681 tctgctgggg ctggagcatc atcatctgga ataaagtcac ctaaatggct atcttcttct
     4741 tctcctattg gtgtttctaa agacacaggg tcttgagcta ttttcattat ttctcttact
     4801 ttatcttctg tcatttccat ttcttttgca atttcttctg gttttggatc tcttccaagt
     4861 tcttgaagta gttgtcttga tactcttatt agcttattta tagtctctac catatgaact
     4921 ggtattctta tagttctagc ttggtctgca atagcacgtg ttatagcttg tcttatccac
     4981 catgttgcat aagtactaaa cttatatcct tttgtatagt caaatttttc gactgctttt
     5041 ataagaccta aatttccctc ttgtattaaa tccaagaaaa gcatacctct tcctacatat
     5101 ctttttgcta tacttacaac tagtcttaag tttgcttcaa ctaacctttg ttttgctatc
     5161 tcatcacctt cgtgcatcct tctagcaaat tctacttctt catgtggttt aagtagagga
     5221 atttttccta tttcttttaa gtacattctt acagggtcat ctatacttat cccttttggt
     5281 aaagataaat ccacagtttc tatttctatt gcagaagagt catcatgtga aattgcctct
     5341 gcatcttcat ctaagtggcc tatacttaaa tcgtcatcag caaccgaaaa atctatatca
     5401 gctttatagt tttttgtttc tgttatttct attcccaaat ttcctaaagt ctcataaaga
     5461 ttttctactt gatccttatc aagttcagtc tccgaaaaag cttccattat ctctgcaagt
     5521 gtcagagaac cttgcttttt tcctttttct attaatgtct tagcagtaac cttttttaac
     5581 tcttttttat tcgatttatt ttccacactc aaacctcctt tctctacaag ctctttaaaa
     5641 ttttatttat ctcaactatt tttaaagcaa tttccataac tctaccatct acctctttca
     5701 tatcattatt attttctaat tcttgttgtt ctctcaacaa gctattaatt tgctcttcta
     5761 atgagttttt tctgatattt gttattattc cttctatttc ttttgtgttc tctaagttta
     5821 tactattcaa agaaattgaa tttaattctt ttaagtattc ttcggatatg tttaaacttt
     5881 tcaatttgtc aatagttatt ttgtccaatt cttgattttt aatcatgtaa tttaaaattt
     5941 ctttactttc tttcaataaa aaatcacttt cttctacttt aagtaacgct atttctctaa
     6001 tttttttgtc ttctaacatt atttttatta gagtttcttc aactaattgt tttccgtctt
     6061 gtctgacttc gactttttca attacttttt cttctatttt cttttgattt ttgttattat
     6121 atggcttatt gtaattttta ccataaactt ctctctttat agactcgaca ttaatatcta
     6181 tttgattgct taaatacttt gtataaaaat caatttcaac aggacttgtc aattgtttta
     6241 taattttaga agcttctttt gcgaacttca ctcgttcttc atctttttga atgttaaatt
     6301 cttttttaag atgatctatt ttatatttta tgtagtgagt agaaacatcc atagcttttt
     6361 tataatcact aagtccgtat tttcttacaa attcatctgg gtctttagca tcttttaagt
     6421 ctaacacttt tacattgata cctaatttag taagtatatc tatagctctt aaagtcgctt
     6481 ttattcctgc ttcatcagaa tcataagata taatagcagt gtccgcatat ctctttatta
     6541 aaatgccttg ttgttcagtc aaggctgttc caagggttgc tactacattt tttataccat
     6601 actgataaag cgaaattaaa tccatatatc cttcaactaa gactatagtt ttactttcta
     6661 aattttttct agcaaaattt aatccgtata agttctgttt tttattaaaa attaacgtgt
     6721 ctggagaatt taagtatttt ggcaaagaat cgtctaatac tcttccccca aatcctatta
     6781 tatttcctct ataatcgaat ataggaaaca taactctatt tctaaattta tcatagcaat
     6841 tagttccatc tctatttttt gcaattagac cgcactccaa taagtcttgt tttttatatc
     6901 ctttactgat tagagtattc ataagtgaat tccatgaatc caaagaaaat cctaaaccaa
     6961 atttcttaat aattttatca tctagtcctc ttattcttaa atattcatac ccaagatttt
     7021 tgcttcccaa taagtttgaa agataaaatc tagctgcttc cgtatgaata tcttgaaatt
     7081 tcttagattt ttctatttta atcctagttt cttcattcat gttagtgtta atttctatac
     7141 cgcatttatt tgctaaaatt tttacagcat ccataaaatc caaattttcc attttcatga
     7201 caaaatttat tacgtctcca ccttcaccac aaccaaaaca cttataaatc tgttttgacg
     7261 tatttatata aaaagaaggt gtcttttcgc catgaaatgg acataatcct ttgtaattag
     7321 ctccagattg ttttatattc atatattcag atattatact tgctatatcg catctagctt
     7381 ttatttcttc aataatgtct tttatatcat tcatattatc accttctggt gtaatttttt
     7441 gtgttaacat ttctattcta cactatattc taaatttgtc aatcactcaa tatacacaat
     7501 tcgacttttt ttattgatat ccttcttatt aatcaaaaaa tttttatttt ttatattaat
     7561 tctatcaata ttaaatcttt atttatatta aataatctaa ctatcaaatt ataacttata
     7621 aatttacctt agcaacgatt acgaataaaa aagaggctct tgcctctttt aataattatt
     7681 aattgatttc attaaatctt caaattcatt aaagcaaaga gattgagcac catctgacaa
     7741 agcattttct gggtcaggat gtacttctac cattattcca tctgctccac atgcaaaaga
     7801 tgccagtgac ataggtttta ccaaatatct aactccagtg gcatggcttg ggtctaccac
     7861 aacaggaaga cctgtttcct tttgaataat gggtactgca gctaaatcta atgtatttct
     7921 agtataatca ttatacgttc ttatacctct ctcacacatt ataatattac tatttccttc
     7981 tattgctata tattcagaag cacctatcca ttcagtaatt gtggaagcta ttcctctttt
     8041 taacataaca ggtttatttt gttttcccac ttcacttaaa agagtaaagt tttgcatatt
     8101 tcttgaccca atttgaatta tatcacttat actatacagt tcgtccatat ctcttacatc
     8161 cataagttct gtaataacag ccataccaac ttcgtcttta accgctttta atatttcaag
     8221 accttctttt tttaatcctt gaaatgaatt tggagatgtt cttggcttgt atgctccacc
     8281 tcttaaaata tttgctccct gactttttac aaatttagca gtctccaaaa gttgctcata
     8341 actctctatt gcacatggtc cagctattat aagtttatct ttttttacag ttatcttttt
     8401 gttatttgta tttatctcta tctttgaatt attattaaat aggttttctt tcataaccta
     8461 caagctcctt tacttcttct ctttcttcag cagataggag gtttaaatgt tcttttattg
     8521 ttttttcatt tattataagt tcttcattta aatccataag tggtatcaaa acgaaagctc
     8581 tatcttgaat ccttggatga ggtattgtta gtctttcatc agtggaaact acatcattaa
     8641 aaaatagtac gtctacatct atagttcttg gtccccaatg aactattctc tctctgtgta
     8701 gttctctctc tatttcttga caatattcta acaactcata aggttcaaac tctgtttcta
     8761 tttctacaca catatttaga aaatctgctt gttctgtata tccccatggt tttgtttcgt
     8821 ataagcttga ttcttttact ttatatatag aatcactatt tttcaaaagt tcacatgctc
     8881 ttgataaatt atcaaatcta tcacccatgt tagtacctat tcccaaataa gccttattca
     8941 tctcttgttc tccttatctc aacaccaaaa tagtcataaa taccatttac aggagcctca
     9001 ggctttctaa ctctgaccat aactccattt ataagtataa atttattcaa gacttcttca
     9061 gcaatatttt ccgctaaagc ctccaaaaga ttaaattgtt tattttcagt aatatcttta
     9121 acaacattgt aaacctcggc atagcttaca gatttattta tatcgtcact aagacctgct
     9181 tctcttgaat caatataaag ttccatgtcg acaaagaatt tctgacctag aaaattttct
     9241 tctttaagaa caccatgata tccatagaat cctagattac taagtaatat tttatccatt
     9301 atattatacc tactttctat atattgcatc agtcattcta gctgccatta aattttcata
     9361 aacatcatgt actctaacta tatctacgcc atctcttata ccaagaacag tagtagctat
     9421 tgtaccttct aatctttttt taggctcaac tggaagtact tttcctatta ctgattttct
     9481 ggatgtccca agcaaaatag gataccctaa atcttttaat tcatttaatc ttctcaatac
     9541 ttcaatattt tgttcaaaat ccttgccaaa tcctacacct gggtcaagta ctattttttc
     9601 tttttttaca ccacaactca ttgccatttc tatacttacc ttaaaaaagt cttttattga
     9661 ctccattata tctttgtcat agtttgtccc atcttgatta tgcattatac acacttctgc
     9721 atcatatttt cctattacag aagccatatt tttatctttt ctaagacccc aaacatcatt
     9781 taccatagtg acacctaatt ttaatgcttc ttctgcaaca tctgccttat aagtgtctat
     9841 tgatattggt atatccaatt cctgtttaag tttttttatt acagggatta ctcttcttag
     9901 ctcttcgtct gagtctacat agctatgacc tgggcgtgta gactctcctc ctaggtctat
     9961 tatatctgca ccttggtcaa ccatatcttt tgcatgttgt attgctatgt ccaagttatt
    10021 aaaatctcct ccatcagaaa aactatctgg agttacattt aatattccca ttatataagt
    10081 tcttttacca taatcaaaca caattaatga cactccctta aattaaattt tatctcaata
    10141 aatgattata tctataataa attactactt catagtaatc aaactcatag cctcggctct
    10201 agacgcaatg tccttttcaa atattcctct cactgctgat gtaacagttt ttgaacctgg
    10261 tttttttacg cctctcattg tcatgcacat atgttcagct tctactacta ctaagacacc
    10321 ataaggttgt aattcttcca ttattatatc tgcagcattt ttagtgattc tttcttgtaa
    10381 ttgtggtctt tttgccaatg tatctattac tctagcaagt ttagaaagcc ctgtaagtct
    10441 tcctcctttt ggtaaataag caatatgagc ttttccaaag aaaggaacta gatgatgctc
    10501 acaacatgaa taaaatggaa tatcttttac caatactaac tcttcatgtt tttcatctgc
    10561 aaaaagaact tttaaatggt ctcttggttc ttcactaaga ccagagaata tctcttcata
    10621 catcctagca actctatttg gagtttcgat tagaccttct ctatctgggt cttccccaat
    10681 tgcctctagt atttctctta ctgcatgttg tatcttttct ttatcaactt tattcattta
    10741 aactcccccc attgatttgt gactagaaga ttagttttat aacctctttt gtctcaattt
    10801 ctttaaaaat ataattataa acatctaaaa gtctttgata gtcataattt tcacttactt
    10861 ttgacactag ttcaaaaagc tcattatttt ctttttttaa aacttttagc aattttttgt
    10921 cttttggtac ccatttatca tttatattaa agtacccaat tattatatct tttaaatata
    10981 aattcgtcaa aaaatgatat tcaaaaacat caaatttttc tttatttttt aaattcaata
    11041 tttttgctcc aatatcttgc tttataaaag acttttcctc taatgacatt ttactaggtc
    11101 cttctaagta aatatctcta cttatatctt ttataatatg tgatatattc tccctatcaa
    11161 acagtacttt agcatctttc atttctttca caaagaaata ttccctattt gataataatt
    11221 tttttacacc atcttttgaa aagtaattta catcaaattc tattcccttt tttttaaaca
    11281 gtattctctc ttgtttttcc ccttctttta caaaaacgaa cacatcaata tcgtttatca
    11341 ctacatcata aagagtaaaa tctacatctt tactagaacc cactaaatat acagccaaag
    11401 tgttttggtt ttctattatt ttagtcatct cttcattgta tacttcataa atacaaccta
    11461 tcatcttttg tctccaacaa attgttaata ttttaattta ttctaataac ttcactattt
    11521 tatcaaaaac aacattttct ttattaaatt ttattttgtc aatttctgta acctttatag
    11581 cgcccataag actatttgtg acaaaaacaa aatcaaaact actaatttct gatatattaa
    11641 tttcattttc tatcaactct atatgtaatt cgtcacaaat ctctataatc ctttttttta
    11701 ttattccatt taatatcggt aatctatcac taggagtgta cactctttct ccttttataa
    11761 agaaaatatt gctcatagaa cattctaata ttacaccttc tgaatttaaa aatataccat
    11821 cattataacc agtcttattt gcgatatttt tggtatatat gttttcaaaa taattagtag
    11881 tcttatgtct atgaattaaa ctattccccc taactattgg agatatagct aatttaaacc
    11941 ccttctcata cgtctcttga ttatatggaa tatccctaat agatatatta taaccttcat
    12001 caaaaacagt aattctaagt gctttattaa tgacattttc tttttttata tatgtaacaa
    12061 tttcatttat caagaaatct tttttatagt ttatatctaa atccaaacaa tttattgagc
    12121 ttagcattct ttctatatgg atatctaaat ctactgctat tccattttta actctaatag
    12181 tttcaaacaa accaattcca aatttactta aactactatc aaaacctaca ttattcttca
    12241 taatatcccc tcaaagcctt cattatggat ttagctttat caagtgtttc ttgatattct
    12301 tcatctgggt cagaatccca agtcattcct cctccaactt gaaagtagac ttttttgtca
    12361 tttttgatta ttgttctaat tgctatatta aaatccatat caccattaaa acctatataa
    12421 cctattgaac cagtatatac atttctttgc gttggctcta attcgtctat aatttccatt
    12481 gctctaatct taggtgctcc tgttatagac ccccctggaa aagttgcttt tattacatca
    12541 gtagcatctt tatcatcttt taactcacct acaactgtgg aaactaagtg atttacattt
    12601 gcatatggtt caattacaaa tagctctgga actttaacac ttccagtttt agatattctc
    12661 ccaatatcat ttctttctaa atctactatc ataagtagtt cagctctatc tttttcacta
    12721 tttctaagtt cttgttgaag tcttaaatct tcttctttat ctttccctct tggacgagta
    12781 ccttttattg gtctagtttc tattctctta ttaacacatc ttataaatct ttctggagaa
    12841 tttgataata tatgtgcatc ttcaaagttt aagaatgccc caaatggtgc tgggctaaat
    12901 cttcttaagt ccctatacaa ttcaaagctt gtaagttctg tttctccact gaacctttgt
    12961 gttaaattag cttgatatat atctccttgt cttatgtact ctctaacact ttgaactgca
    13021 tttttaaatt cttcctttgt aaaattagat ttaagtctta taggtgttac ttctttttct
    13081 tcataacata tgctatctat accttttttc tcttctttta atattctttg ttctatatca
    13141 tctattatct tttcttctaa ttcgatatct atgtttggag tagctatata agttttttgc
    13201 acaagatggt ctataactat tacatgatta taaaatccaa aatacatatc aggcatctca
    13261 atatcatcta cagcagttct aggtaaattt tctatgtaat tacctaaatc ataagataaa
    13321 tatcccacag cacctcctac aaatggaaga ttagtatcat ttacaaccct atacttatgt
    13381 agttcttctt ttaaaacttc taaaggattt tcatcagtat ctttatactt taatacctta
    13441 aatggttggc tacttataaa agaatatctt cccaattttt ctttatccat agcactatct
    13501 aatataaaac tgtcatgttc atttctaaaa atagtaaata tctcaaaaga atttaattta
    13561 gtatttatct ctcttatcat attacatatg taccctttct ctagcttctg ttataaaatt
    13621 tttaagcatt tcatgaccat actctgacaa aatagcttct ggatgaaact gtactccttc
    13681 tattaaatat ttcttatgcc taattcccat tatttcccct ttatcagtaa ttgctgtaat
    13741 ttctaattct tttggaactg tattggagtc aactattagt gaatgatacc tagtgacatt
    13801 taaaggattt ttaagtcccc taaaaactcc tttattagta tggttgatac tatatacttt
    13861 tccatgcaca ggctgttgtg cttttataat atctcctcca aaaacatgtc caatagtttg
    13921 gtgacctaaa caaattccta gtataggttt tttcccctta aaatgctcaa caatatctat
    13981 gcaaatacca gcttcttttg gagaacacgg tcctggagat aacacaatta cctctggatt
    14041 tagttcttca atgtcactta ttttgatttc attatttctt tttactacaa ctgcttcccc
    14101 taattcttct atatattgga ctaggttgta tacaaaagaa tcataattgt ctatcataag
    14161 aatcatactg taaatctccc ccctaaaaat tattttatca actcagaaac tgttacaaat
    14221 ttaaaacctt tttcaatcat ttttggaata aacatatcta aagcttcaac tgtatcatta
    14281 gttgcataat catgcaaaag tattatatct ccattttgta ctttattaat tattttatct
    14341 gcaatgctat tagctcctgg atttttccaa tctttcacat ctacacctgt ccataaaaca
    14401 actttatagt catgcttttt tgcaatttct attaagtctt tttctctata acttccgaat
    14461 ggtggtctaa ataatgtagg ttttttccca gttacttcct tcaaaatgtc ttcacactta
    14521 acaatctcct cttctatctg actagaactt aaattactta tatcaggatg attgaaagta
    14581 tgatttccta tctcgtgacc ttctttactt gctcttacta atggttcttt ataccatttt
    14641 gcatgttttc cagctataaa aaaagtagct tttacattgt atttctttaa tacatctaaa
    14701 acttgactag tttctttagg atgaggacca tcatcaaagg taattgctat tagctttttt
    14761 tcccttgaac catgtttaat caaaacattt ccattagtat ccatcatcaa agcagaaact
    14821 ttactaaaat tagatattaa cactattcca caaaacaagg ctactgctat taagcactta
    14881 tatactgtct tttttttcca tgatgtcatt ttttaacttc ctttcaaata ttttagttat
    14941 ttataaaaat tttgagtgta atatgtttta tagcttcctc caaatattac tccaactcca
    15001 atattattat agttccccaa aatatttttt ctatgtcctt cagagttcat ccaagcttca
    15061 tgcgcatata ttgcattaat ctgtcctgct gctatgtttt ctccagctga agtatatact
    15121 ataccttctc ttttcattct gtcaaatgga gtttcattct tcttatttac atggtcaaaa
    15181 aaattattat ctctcatatc ttcactatgt tttcttgaac tcaaagttgc ctgttcactg
    15241 tatctcaatg agtttaaccc tctttgattt ctaacactat ttacaaggtc tataacttca
    15301 agttcaaaac attttttaag ttcttcgcta tcatctggat ataaattttt aaactcatct
    15361 tcagtgcttt tatcaattat taaatatgaa caaactctat tttcttcatg aatatcgtag
    15421 aaaattgtaa tatacttacc ttccttactt attatatcat attgattttc tgagtttatt
    15481 atatatctgg tatttccttt tctcttatat tttaatggtg taatcttagt tctcactgtt
    15541 tgtctatctt gatttagttt tatatcttga ttttcacatg aattaataga attactataa
    15601 agtgctacta cttcatcatt ttcaagtcca accattgcaa attcttttcc atattgatta
    15661 tatacatacc aatcaaaacc atattcacta atatcaattc taccaggttt gccgatactt
    15721 gataaaacat agcttaaatt atctcctaag cttatttttt caaaattctt tatatctgac
    15781 tctcctaagt tctccccttc agtattattt gattgtaaat tttgactttt ttctttttta
    15841 tctttcttag aattatcatt tatatttatt ttgtctaaaa attcattcca tatttcactt
    15901 tgaaaaaact tttcttttaa atcatatcct tcttttaata attcaggact ataaaataaa
    15961 acaataagta ctactaacac taaagcaatt aatctcttca atatatctct ccttcttaaa
    16021 atcaatcaaa attttttaat attgcattta ttaagttaat attgatagtg tatactaatc
    16081 aatatcattc atacctattt acagtaagta ttagaaaaaa gctttatcta ctaattagat
    16141 aaagctacag tctcaaggaa aatatttcat ctaatgttct atcaaatata gatggcttat
    16201 caaatttcca agtcccttca taaaccaatt cattatttag ataaaaagtt ccttgcccat
    16261 gttttttacc atgcataaat tcacctatat actttttacc tttttcccat atataagttc
    16321 ccttaccatg aatcaaatta ttcttccatt ctccttcata aagtacttcc cctttactat
    16381 tatagcaaat accgtaccct tccatctttc cacctttaaa ttcacctgca tatatacatc
    16441 catcactcca cttataaata ccttttccat ctttttttcc gtttttccaa gttccaatat
    16501 attcatcttt actactatat aacatatttc caataccgct catcatatct tcctcaaaat
    16561 aacctatata ttcatttcca tttgaaaata taaagtttcc ttttccattt tttttatctt
    16621 ctttaaattc tcctatgtac tccataactg ttccttcatc tacaaaaaaa gtatatgagc
    16681 ctttcccctc catctttccc tctaagaatt tccctacata tacatcccca tttgaaaatt
    16741 tatagatttt attttttggt ttttttgtat cgacatcatc tatatcgcgt attgtttttc
    16801 cttccaattc taatacttca ttttccggag caaaatcgcc cctttttaaa acacgtttct
    16861 tcatgttctt tatacttcta atctctttgt ttacttcatt tatatatttc aaataattat
    16921 catacaaatt aacatattct ttagataaat tcataatgcc attcctccaa cgttattcct
    16981 aggtatttta ctaaatctat taaatttatt atatcctaat agtggtcatg ttttctacat
    17041 attaagtaat tttttcaatt ttaattttaa aatgatatct tttaaagtga tacacaaatc
    17101 attaataaaa cctttatttt cgtatactaa atttaaataa gctgagagat ataatataca
    17161 aatttacatt ataaactccc agcttgtaaa attttctata aatcttttgc tttaaaccct
    17221 cttcccttta cttctgtaac atcatttaca gatataaagg cctttttatc atacttcaaa
    17281 gctatatctt ttatctttgg tatctcattt gatgaaacta ttgtatatat aatcttcttt
    17341 ttttgatgag tgtatgcacc ttctgcatct aaaaaagtta cacctctaac taaactcttc
    17401 attatatctt ctgctattaa atcgacttca tttgacatta ccataacaga tttttggtaa
    17461 ttcattgcat ctttaataaa actcattgaa tacgcattta gaaatagacc tataagagtg
    17521 tataatgcta aatccaaccc aaataaaaaa cttcctgtaa gtactatcaa gccatttatt
    17581 gctaaagcag tgtctttcat ttctatattc ttcttaattt ttagtattgc agctattata
    17641 tctgtaccac cttgagaaga cctagttcta aatacaactc cgactcctaa accagataat
    17701 ataccaccat atacactttg aagcaatata tcatgtatag gtataatatt acttatttcc
    17761 tgagttgctc ctaataataa tgaaaataca atcatattta ccaaacttga aatacaaaac
    17821 tctttttcaa ggtatatgaa ccctaagata aatattggaa tattgattaa aaatgttaat
    17881 aaacctacat ttatatttgt caaatagttt atagatgttg ctatacctgt agcacctcca
    17941 ctaagcagat gtgctggtct taagaaccca tttattccta tagatgtcat caacatccca
    18001 aatagcattg ttgaatatct taaaactgga tgtttttgta gggtattctt aacttcactc
    18061 atttctttct cccttcaatc tttctaaatc tttagaattt taaaagctta ctcttacata
    18121 tattattcct gatgtaatag ataatatttc aaataaattt tttttataat ttcttgaact
    18181 gtcatctatt acatcatcta ttatattatc tttttcatat atgtctagtg caatctttta
    18241 tattctaatc tttaaaaacg ttcaaatcat ctcatttatg ccttaaatga aactttttct
    18301 agcataatat gatactgctt attatttaac ttgcaatata gtaaatttat agtatttttt
    18361 tctaaatatc tattttagca gtaatttatt ttattttagc aaattcaagt ttagtaattg
    18421 tctctttata attaattcct aaatgaaatt tttcttttta taatttcagt acatgatttt
    18481 tttatttcat tatttgcatt tttttcgttc agtttttatt ttaaattttt tttattttat
    18541 tttgttttaa gtattcaagg tactcatctt aattttattt tttatataaa attcattata
    18601 attggaatta gtattactgt aattgtacca gagactccaa tagaaacacc actcatagct
    18661 ccttctattt ctccaagttc aaaagccttt gtagttccta aagcatgtgc agatgttcca
    18721 agagcaattc ctttagcaac tggatgattt atattcccca gtttgaatac tgtagaagca
    18781 accataccac caaaaatacc tgtaaaaatt atggctacaa ctgttattga ttctacacca
    18841 tttaaagaat ttgctaaaga tattcccatt ggtgttgtta tagattttgg aattagtgaa
    18901 tttattatac ttacatctga attagctatt ttgccaatta tcaatacaga tacaaaagaa
    18961 atcactatac cacaacctat tcctacaata atctcaaaca tatgcttttt aaataattca
    19021 aattgtttat aaagtggaac tgcaagtact actgtgacag gacctaaaaa gaaatttaga
    19081 ctatcagctc ctatcttgta attctcataa gggattttag atatgcttaa aaacaatatt
    19141 attcctacga ttgcaattaa taaagggtta catataggat tacttgtttt tcgttgaatg
    19201 tatcttccta aattgaaaaa aactattgtt actattatac caaatactgg tgtctgtaat
    19261 acattataca tttttttgtc cttcttcctt cttttttatc attttctcca caataattcc
    19321 agtagtatac attactatta gtgtacttaa acatatgact aataaaagca ctaatacatt
    19381 ttcccttatt aggtctaatg actttataag tgatacacct gctggaataa aaaatatagc
    19441 catattatcg aggaaaaatc cacttacatt ttctatacta ctcaagtcta aaactttaaa
    19501 ttgtagtagt agaaataata atagcatacc aactatactc ccaggaataa gtataattcc
    19561 ttgtataaag gaacttatat actctcctat tacccagata gataaaatta ttaccaattg
    19621 attgaaaact ttcattttcc acctccaaaa attaaagaga ttgcttcaaa tcatatattt
    19681 gtagttttat caacaagcaa atatctttct ttaaaataga aagttataat ttgacacaat
    19741 ctctttttaa atttattttt attttaattt aataaaatac atacttggtt gtttcaaaaa
    19801 acttaactat ataaacctat ttttcactta aaatttcttt tatttttgaa ttgttataca
    19861 tactgtctgg aacttcactt ctcaaactat aaaaagtctg ttgtgcattt tttaaatcgc
    19921 cattatcata ataaagcata cccaactcgt aatatgaatc atcataatac tggtcatttt
    19981 tagtataagt atttatatat tttttgtaat attttatagc ttcatcctta tttcctaata
    20041 atttatttgt tattgctaat tgatatattg actcagaaat atatttttta gttttaccag
    20101 aagaaactac acttttaaaa ttatctatgg ctttttcata ttctttgttt ttctttaaat
    20161 ttagagcttg ataatataaa tcttcctcat cagctacagt tatctctctt tctggttctt
    20221 tatttttaac ctcatttaat tccttattcg ttttttctaa ttttttactc gtttctccta
    20281 actttttatc agtttcactt aacttttcct ctttactact tatttggtta ttcaagtttt
    20341 ctattctctt tttgctttga atcaaatata tccccatacc tattaaaaca cagccaacta
    20401 taatatatgg caaaatactt ttctttcttt ttactttttt tatttcttcc tttttacttg
    20461 tatcttcttt ttcttcttca tttttttcaa atacaggatt atctttatct agctctttaa
    20521 gtttgtctaa atatccttct ctctttttta cattacctag tttatcatat aacaaagata
    20581 aaattacata tggctctatt agttcaggtt cttcttcaat tatgttctct aaaatatgta
    20641 ttgactcata atttaattct tcatttataa atcttataga atgattgtat cttcctaaaa
    20701 aagttttaaa atcttcagat gaaattagat ttacatattt tctagatata tgattatttt
    20761 cataacataa agcagtataa aaactctcaa atgatttagc aaaatcacat tttaataatt
    20821 ttaaaagacc tctaagattt agtatatcaa tgtctttagg atttatctcc agagctttat
    20881 taaccatatt aatggcacta gttatataat ttttatatgc taaatccaat gcattgttat
    20941 atcttttata tgattctaat tttaactcct tagacgttgt atctgacttt gaatttaaag
    21001 ctccttgcaa gtttaataga gtttcttctt tataatttgt agtcacataa ctctgtttta
    21061 atgcttttaa tgtttcatca ccgtcttctt cttctatatt aaaaaaagtc aaatcacttt
    21121 tattatcttc tatcaaagat atatctaaaa tttttctagg catgctttct atgtcttgtt
    21181 ttatatcttt aactaaaata atactcacct ccactctaaa atatatattt aagataacat
    21241 atttaatatg tcaagtcaaa actatacata tttttccacc attctcataa taaaaaaggt
    21301 aatcctcttc ttttcataaa aaggttacct ctttttcaat actattaact tttaatgttg
    21361 aattttttta cttttctata gagtgtagct atacctatat ttaatgcctc agctactttt
    21421 ttcatacctt ctgtattact tccatatttc tttatggcat tttctatagc ttccctctca
    21481 aggtcatcta tagtcttaac ttcttgttta taaagttcat ctagtttaaa agatattaaa
    21541 tcagacatac tccttacaaa agcagttaac tccttatctt ttgaaagtat cttttctttt
    21601 tgttcttcat taaacgcagc tatacccaag attccaagta tatgctctcc cattttaacg
    21661 ggagtacata tatcagctaa ctctttacaa ttttctctat ttgcacattt attacagttc
    21721 tcttctattt ctttatttat aacataggat ttcccatctt ttaaaacaag agagtacact
    21781 gacttctctg atattgtctc tcctatttta cctctgaata ttcctgtacc tgctatccta
    21841 gttaaatctt ttgtaactaa agtaacatct acatccaaga cattagaaat attctcgcaa
    21901 atactttgaa cgtaattcgt tatattattt aaattcaaag taatccctcc tatttagatt
    21961 tatatttttt attactctac ataaatccta ttcttgagaa ctttaagctt gcggttgtgg
    22021 ttgtgtctca ttaggcttat tatcagttgg tggtgtttct ggtttttgtt gattatttgg
    22081 gttttgattg tttgtatttt gattattctt attttcgtct gtatttggtt tttcctctgg
    22141 ttttggttcc atagtcccaa ctaatataat accttccttc ttaggataat atgaattagc
    22201 tattttttca gttttaattt ttttaccatt tgcatcatta aatgttctat atgttgatac
    22261 agaatatgca tttcttccac ttttgtctac tttttcttta cctttaggca ttgtagggtc
    22321 attaactctt ttcattgtag tttgtgaaac gccatctaat tttgttgata ttgttatatt
    22381 ttgtttatct tctgcacttc catatatatt gcaaacaatt tgatttcctg aaacgtaatt
    22441 ttttatgtaa actggatgct ttagattatt tttaaataag aaatctattc cactatctgc
    22501 aacagttgca tctcttcctt ttggaacata acttgatggt atagtatgat tttttacatt
    22561 aacaatttct aaacctgcgt atagcacaga attgtataac gtagatgaaa cttgacaaac
    22621 tcctccacca taatcttctt ccatctcacc ttccattatt acaggtgcac ttttatatcc
    22681 attttcctta cttcttttcc ctgtatgttt attaaatgag aaagtttctc ctggcataag
    22741 taatacatta cttatattat ctgttgctaa ttttatgttt atacttctat ttatttgaga
    22801 agttttaaat gttgttgaat acgtacctaa atttgtatct atattagcaa gttgctcttt
    22861 ttttactttt gctggagtag cttttactac taactcattt ttatagttac cttttttagt
    22921 ttgattatca aaattctcca tactcttaga aatatccatt ttaagaccat cagaatctgg
    22981 tataaccttg actttttcac cacttatatc aagtgtagca tcttttacat ctacatctat
    23041 ttctttagct atcttttcca tttctgcttt taatttattt ttatcataat ttatcgcaac
    23101 tactaagttg ctttttttgc caaaatttgc tgaaatagtt tttgatagat tgccaaagaa
    23161 agcactcttt ctatttaaat tatatgcgtt ttctactgtt ttatccaggt cagtagataa
    23221 atttaaatct ttgctcttta ctttccagct tttatcattg taattaaaat caacattctc
    23281 taatttgtat tttttagcta attcctgttt tgcttgtgat ttagttaatt ttccaatatt
    23341 tactccatta acatatgtgt tttttgctat tttctcccct ttaaattgca tgcacattat
    23401 gcctataaaa attattacta ctaatgctac agctccaata gctataacta gctttttgtt
    23461 cacattactc atcccatact tctccttaca ataaaattat ttatatatcc ctattattat
    23521 attactacat aatgcgcctt aaaaccccaa aaacttttta attatatatt aatttaatat
    23581 tattaattaa tattcattta gtccattttc tgatatggca tatatttttt cattgtaaat
    23641 attaatagca taaacttctt tgcctctaaa taagccttta tttactacag cataataata
    23701 aatattttta tattttccaa acaattcttc taaactcttt ttacttttta catatctatc
    23761 tgctttctct atatctaata tctcaatctt tttatactta ttttcttttt catcaagagt
    23821 attagttaac acaaacttag ctttatcttt tgtaatacca aattgataac ttccatcttc
    23881 taatattcta aagtatccat caccataatc tactttgtaa tcttttttac catctttata
    23941 tgaatataaa tctttagttt ttgcacttac caaaacttca tttattaatt tatcatactc
    24001 atatacttca gcataatagt atttattatt ttttatcatt tgtggaagtg aatttataga
    24061 tagatttgcc ttgtctttgc cacttttttt agctattaca tttataatgt catcagatgt
    24121 taaagtatac aatgataaat tctttttata ttttttttct aaactctgta ccttttcatc
    24181 atctgaataa tcgtctttta aatactcaat atattgtaat gctctttcat aatctccaga
    24241 agtatttgcc tcttcagctc tacttatata gtaatcatac atttcttcaa tacattgttc
    24301 tttcccattt tgagccatac tgtaatactt ttcatcttct tttaatactt tattatactt
    24361 ttcaatagct tcataatact tatatgcttt ttggtctttt actgctgagt cataaacatc
    24421 tctagactct ttgattgttt ctatattctg tttgtataca tctaggttac tacccatacc
    24481 atttaatatt gaaacagtac ttatgtagtt aatggcaata tcataagtag tattttcttc
    24541 tttatacatt tctctaactc tatcagcttg agctaataag tcatccacat ctatacttat
    24601 tttgtttaat tctttcacgg tatttattaa tccaataaaa ctttcttgac ttatgtctcc
    24661 atctaaatat ttatcaccac tatttaatag caatttattt atttttttag aaacagactt
    24721 attaaacctt tccatttttg aatcagaaaa ctttttttct atacttgtgt agtaatcaac
    24781 agctatagaa tactgtcttt tctctataat ctttaacaac tcattagctt catttcctac
    24841 actaaactcg ttactactta agaaaaatac tattatcaaa gcaattacac ctaatgttga
    24901 taatgaaatc gcaactatag atttcctttt ttcatcaata aaatctacag ccttctcaaa
    24961 taaatctcta aaattcatta tagttccctc tccatttaat aatctttata ttttatggta
    25021 aactaaattt cttattggtt attatatcat aattcttgaa aaaaaataaa aacacaagtt
    25081 aaatcgtgtt tttattttac ctatcaatca ttattttata cataaatatt tcctttacat
    25141 aaattatgat atctttcttt ttatatccct agaatgactc ttttgttttt tattattact
    25201 gacagttttc gttcttgtct tgtgtatagc tttgctatca aatatttttc tagcattgca
    25261 taaaacaatt ccaattgtta aacaaactgt aagcacactt cccatcaaaa agtaataatc
    25321 caatcctata tgtactccca taactgaatt tattaacact aatgcaaaag tcattgcaaa
    25381 acatatcatc atggagataa ctaaaattct tagaattttt ttcaatccac tcacctcgta
    25441 taatcactaa aattgacact accccttatc cattactata ccaaattagt aaaaaaatag
    25501 caattactat tgtcatttat attaggaaaa tattaaatct ttttaataaa tatctttttt
    25561 tgacataagg attacttaac ttttaataaa atagtaacac taatatgtat taattaatag
    25621 aataaaataa atgataattt attgaggggt gatttacatg ccaaatttgc caagtttagg
    25681 gtcaaaggct cctgatttta aagccaatac aacaaatggt cctattagac tctctgacta
    25741 taagggtaat tggattgttt tattttcaca tcctggtgat tttacaccag tttgtactac
    25801 agaattttta tgttttgcta aatattatga cgaatttaaa aaaagaaata cagaactaat
    25861 tggtctaagt gttgatagta acagttcaca tttagcttgg atgtataata tttctttact
    25921 tacaggtgta gaaattccat ttcctattat agaagataga gatatgagaa ttgccaagtt
    25981 atacggcatg atatcaaaac caatgagtga tacatcaact gttcgctctg tatttattat
    26041 agataataat caaattctaa gaacgattct ttattatcca ctaactacag gaagaaatat
    26101 tccagaaata cttagaatag tagatgcact tcagactagt gatagagata atatagttac
    26161 tcctgcaaac tggtttcctg gaatgccagt gattttacct tatcctaaaa actataagga
    26221 attaaaaaat agagttaaca gttgtaataa gaaatattca tgtatggact ggtacttatg
    26281 ttttgtacca gataattata atgatgaaga agtgagcaag aaaattgata atacttgtag
    26341 ctggaaaaaa gaacatacta aaaacattga aaatgaatgt aactgtgaac atgaacatca
    26401 tgactacctg aacaaagctc ttgactgtaa acaagaacac aagactgata ttaaagatga
    26461 ttgcaatcat gagaaaaaac atactaaaaa tactaacaaa gttcacaact ccaaacaaga
    26521 taagtttaaa gataagtctt gtgatgaaat gaattttaac tatgataaag atgaatcttg
    26581 tgacaaaata aattctagct ataacaaaga agatagtagt tatgaagatt tctataaaca
    26641 taattataaa aactacgatt atactagcga aaaaaatact aaaaaaatag ctatgaaaac
    26701 tttaaaagat tctaaaaaat tagttagacc acaaataaca gacccataca atccaatagt
    26761 tgaaaatgca aactgtccag atataaatcc aattgtagca gaatatgttc ttggaaatcc
    26821 aactaatgta gatgctcaac tattagatgc agttatattt gcttttgctg agatagacca
    26881 gtctggaaat ttgtttattc cttatcctag atttttaaat caattacttg ctcttaaagg
    26941 tgaaaaacct agcttaaaag taattgtagc tattggaggt tggggagctg aaggattctc
    27001 tgatgcagca ttaacaccta catctagata taattttgca agacaggtca atcagatgat
    27061 aaatgaatat gctttagatg gaatagatat agactgggaa tatcctggaa gtagtgcatc
    27121 tggaataaca tcaagacctc aagatagaga aaactttaca ctcttactaa ctgccataag
    27181 agatgttata ggagatgata aatggcttag tgtagctgga acaggagata gaggatatat
    27241 aaattcaagt gctgaaatag ataaaatagc tcctataata gattatttta atcttatgag
    27301 ttatgatttt acagcaggtg aaacaggccc aaatggtaga aaacatcaag caaatctttt
    27361 tgattcagac ttatctttgc caggatatag tgttgatgca atggtaagaa atcttgagaa
    27421 tgctggaatg ccttctgaaa aaatccttct tggtatacca ttttatggaa gattaggtgc
    27481 tactataaca agaacttatg atgagcttag aagggattat ataaataaaa atggatatga
    27541 atatagattt gataatactg ctcaagttcc atatttagtt aaggatggag attttgcaat
    27601 gtcatatgat gatgctttat caatattctt aaaaactcaa tatgttctta gaaattgtct
    27661 aggtggtgta ttctcatgga catcaactta tgaccaagca aatatactgg ctagaaccat
    27721 gtctattggt ataaatgacc ctgaagtatt aaaagaagaa cttgaaggta tttatgggca
    27781 attctaatag aatgtaggaa aattttatag ttatataaaa agcagatttt attatattaa
    27841 ataagttgta tctaattaat actattatta attgtttata aattagatac ttattttttc
    27901 ataatacaat ctgctttttt aatcaaaatt tatttaatta taagttgttt aattacaagt
    27961 tgtttaatta
//
LOCUS       DAELNY010000035        27726 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.035, whole genome shotgun sequence.
ACCESSION   DAELNY010000035 DAELNY010000000
VERSION     DAELNY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.035"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(114..1994)
                     /locus_tag="KPE55_001295"
     CDS             complement(114..1994)
                     /locus_tag="KPE55_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG0492388.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2198..8161)
                     /locus_tag="KPE55_001296"
     CDS             complement(2198..8161)
                     /locus_tag="KPE55_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG0492389.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8285..8370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8542..9945)
                     /locus_tag="KPE55_001297"
     CDS             complement(8542..9945)
                     /locus_tag="KPE55_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG0492390.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10407..12302)
                     /locus_tag="KPE55_001298"
     CDS             complement(10407..12302)
                     /locus_tag="KPE55_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG0492391.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(12929..13072)
                     /gene="scfA"
                     /locus_tag="KPE55_001299"
     CDS             complement(12929..13072)
                     /gene="scfA"
                     /locus_tag="KPE55_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG0492392.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(13138..13500)
                     /locus_tag="KPE55_001300"
     CDS             complement(13138..13500)
                     /locus_tag="KPE55_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG0492393.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(13558..13851)
                     /gene="yajC"
                     /locus_tag="KPE55_001301"
     CDS             complement(13558..13851)
                     /gene="yajC"
                     /locus_tag="KPE55_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG0492394.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(13985..15106)
                     /gene="tgt"
                     /locus_tag="KPE55_001302"
     CDS             complement(13985..15106)
                     /gene="tgt"
                     /locus_tag="KPE55_001302"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG0492395.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(15185..15670)
                     /locus_tag="KPE55_001303"
     CDS             complement(15185..15670)
                     /locus_tag="KPE55_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492396.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(15682..16707)
                     /gene="queA"
                     /locus_tag="KPE55_001304"
     CDS             complement(15682..16707)
                     /gene="queA"
                     /locus_tag="KPE55_001304"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG0492397.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(17025..18044)
                     /gene="ruvB"
                     /locus_tag="KPE55_001305"
     CDS             complement(17025..18044)
                     /gene="ruvB"
                     /locus_tag="KPE55_001305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG0492398.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(18151..18753)
                     /gene="ruvA"
                     /locus_tag="KPE55_001306"
     CDS             complement(18151..18753)
                     /gene="ruvA"
                     /locus_tag="KPE55_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG0492399.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(18746..19255)
                     /gene="ruvC"
                     /locus_tag="KPE55_001307"
     CDS             complement(18746..19255)
                     /gene="ruvC"
                     /locus_tag="KPE55_001307"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG0492400.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(19583..20212)
                     /locus_tag="KPE55_001308"
     CDS             complement(19583..20212)
                     /locus_tag="KPE55_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492401.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            20415..20738
                     /locus_tag="KPE55_001309"
     CDS             20415..20738
                     /locus_tag="KPE55_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0492402.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21006..21860
                     /locus_tag="KPE55_001310"
     CDS             21006..21860
                     /locus_tag="KPE55_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492403.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(22046..23482)
                     /locus_tag="KPE55_001311"
     CDS             complement(22046..23482)
                     /locus_tag="KPE55_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG0492404.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(23553..24935)
                     /locus_tag="KPE55_001312"
     CDS             complement(23553..24935)
                     /locus_tag="KPE55_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0492405.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(25071..25976)
                     /gene="garR"
                     /locus_tag="KPE55_001313"
     CDS             complement(25071..25976)
                     /gene="garR"
                     /locus_tag="KPE55_001313"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG0492406.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGIDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            26372..27043
                     /locus_tag="KPE55_001314"
     CDS             26372..27043
                     /locus_tag="KPE55_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0492407.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            27146..27694
                     /locus_tag="KPE55_001315"
     CDS             27146..27694
                     /locus_tag="KPE55_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492408.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         8460 a         4967 c         3144 g        11155 t
ORIGIN      
        1 acattttaac ctcaatatta ttagattgtt aagataatga aaataaaata agaatgcatt
       61 taaatatata actttatatc taaatacatt cttattttct atttacttaa tatttatttt
      121 tcaattaaac tcttaatctt gtctattaca gatgaggcta ttcctccacc tacttcaaac
      181 aactttgatg aatttttacc ttttaatgac gattcttgtg atttatctaa cgtatttgta
      241 gcaagtataa ctggtgaatt gtttttagct gctaatggtc caactgctaa agcatctata
      301 agtttatctt gatttgcaac tacaactcca tctagcttag attgagagta gaatttatct
      361 attactttgg cattcgtctc ttgtctagtt tgacctgcca ccctattgtt tgatatatct
      421 ttgtttgcta tttttccaac ttgttcaata gccttgttag atattttttc tactccacca
      481 ataaaatata tatttgacac cttatttgat ttaataaatt gttcagcttc attatctaca
      541 ccatcttttt gtgtaagtac tattggagtt ttttctttac cagccagtga tgctattgat
      601 aatgaatcag cttctccatt tcctgctcct atatacaact tgcttacatt attcgtctta
      661 tctatttctt ttgctatatt aatagaagtt tgatatcttg tatctccacc tattctttgt
      721 actgatattt ttgaatttac agctttgata gcttcaacaa ctgaatttgg cattgagttg
      781 tttccaccta tcacaattac ttttgatgga tttagccttt tcaattcttc tacagttttt
      841 tgaggaagct tttcatttga tgataaaaga attggtgagt tttttacact agcaagtggt
      901 gtagctgtaa gcccatccac catactttta tcttctccat ttactattac aactgtatct
      961 gattttgtcc aaccactttg agatattttg atagctgtct caaatctatc agcacctttt
     1021 aattttttga ctgtgacatt agatgtagaa tcacttgaac ttcctcctcc agagcttcca
     1081 ccactagatg agccgcctga attacttcct ccaccattat ttccaccatt gccattatct
     1141 ggtggtaatt catccggttt tgataattca ttttcaacta tgtattctaa actttttgga
     1201 agtgtaactt caacatattt gtttattgta gcttttcttc ccttagttgt tttttgtgct
     1261 aatccaagtt ttaatattac atttgaacca ctttcttgtg tttctttact aacaatagaa
     1321 tagtttatcc taatattgtt taataaatat tcaagtgctt cacttttctc taccttatac
     1381 ttttcagggt catttttact agaaccagtt ccaccaattg gttttacttc actctcatta
     1441 ataagggtaa atattaatcc ttcatcagag cttatatttt cataatccac tgttttgttg
     1501 ttatatgtta caatttttaa agctggttta tcatttgttc cttttcctaa aactttatag
     1561 tcagcatttg agttactgcc agattttttt acagatattc ttcctatatt caatctattt
     1621 ccgtctagta aatcttcatt tgatgtaaca tatatatcta atgtagttgt attacttgat
     1681 ttgttgtatt ctactctaca agtagcattt gcatttccac tttttatctg agattttata
     1741 ttatcagacc aagtaatctt ttctttctgc tcattatcta ctacttcaac ctcaccttgt
     1801 acttttatac tggcttgaaa tgaatatact atgttttcta ctgagtccac agatatatct
     1861 atagtattta ctttctcata cgtttttgtt ccttgtaaaa ctggagctgc atatgcacat
     1921 gtagcactac cagttacagc tactgccata gttgatactg ctatagttct tataaaagat
     1981 tttcttttac tcattaacac ccgtccttat ctatcattaa ctaagtggat aaatttactt
     2041 atccacctag cttttatatt taatttataa attaccctaa atttatggtt acatttatat
     2101 actttacttt taatatctac tattttaaaa ctattctagt acctaatctt attagatata
     2161 caatgttagt atttttacgt ttttagagtt ccacctacta tttatctcta acaatattac
     2221 cctttacttt attgcttaca gttataacct tcttattact gatatcaata gaatttcttg
     2281 tagcttttct agctttacta ttactatcag taagacttat tgtctttaac tcatcataat
     2341 tcttagctcc tacaggtaca tacattgtat cacttacaaa tctttgacct actggagtat
     2401 ctccttgaag gtcattgtat ctatatagtt cagccttaac cttatcactt aaagaatcaa
     2461 agttttcttt tggcatatag taaacccatg tacctttact gatttcacct agttcacctt
     2521 tttctggaac ttctgcaaat aaaagtgctt ctccagatat agtcttattg tttttatcta
     2581 taagtagtgg taaattaaat gctggatttc cactgtattc acctgtaata actattgaac
     2641 ccgctggaat cttacctgtt tcatcttcac ttgtgctaga gtcatcactt tgatatacat
     2701 aatcttctgc taattttcca attccattag tacgagtttc gtctgcatta tttattatgc
     2761 caatttctat attatcacct ggaggtccta ttatatctaa ttctgcaact tctatattag
     2821 acttattatt tgagattagt tttacatatc tagcttggta tgtccaaagt ttaccatctt
     2881 tattaaatgt aactctagct tcattatctt ggtcttttcc tccaccaact aaatcttgtc
     2941 catattcaaa agttccttga cttacatctt cccagttctt tttatcatta cttacttgta
     3001 tcttgtaatt agttaaagga ttgtatgttg tgctagtatt ctttctaaag ttaaataatc
     3061 cttttaatga gaactttcta gcttctgtct ctggttttgt atattttagt cctactatag
     3121 gtaatgtttc tttcatgtct actattatat atggattttc ttctacattt atatcagggt
     3181 ctttttgagg gtctttgttc cactctgctt tacttattat ctttccttta tatgtagtac
     3241 ttgcatcatt atcttttata ttatcaattt caggattttg taatggtcca tgtgagtcat
     3301 tttcagttct aacatcaccg tcagaaacag tatttgttat gaatgaccat gctgatttat
     3361 ttattttccc ttcatctgat aattttacag taccaacttc aaattcttct gttggattta
     3421 ggtgataatc atatgcaact accttatatg taaatgctct gttattaaga ggagatatat
     3481 tatcagtaaa tttagtcttg ctatttcctt ttgcatccac aaatccaact gttctgtatt
     3541 ttacctgtgt tccattttca ccatctaaat aatttccatc acttcttatt atttcgtatc
     3601 ctagtatctt atctttatcc ttatctacat ctaggtctat agatatttta ttagatttaa
     3661 ctagagaacc actttgtatt ccatcagaaa aactagcact tactttagta tcttttgcca
     3721 tactagctat attttcattt aaaatctttc ttctagcttc atcatttaca tattgtatct
     3781 ttcttgattc tttttcgtag ttcttgctat ccattatact gtttaattct ggtgtaattt
     3841 ctactcccca tgccttaaag taatctctta aatctttttc aacaacactt gatgccattg
     3901 ctactagcat ttggtctttt ggtaaactat taattgggtc actacttgaa agtgctctgt
     3961 attttcttga aatctttgca tagaatgtat cattacttaa atcagcatct gagttattct
     4021 ttaacattga acccgtaaaa tcatcatcat aagctagatg taattgccat aacattccaa
     4081 gcctttcaaa tacattgctt ggtataccta agctatttga agttacatgc tcatatatct
     4141 tatccattgt tgaaccttca agtcttgaat gtgtcttatc atcaaaggtc tgagcaagta
     4201 aagcaagtac attgttacta gtttcgctat atgtcatctt tccaatatct gtaacatgtc
     4261 ctatctcatg acctattccc catccaaata agtttcctcc tgtagctttt ccactatcat
     4321 ctatttggaa aggtactcca ttcataagcc ctggaactga accatattct actcctacat
     4381 gtaatccaga agcatacata aatgctccaa taaacattct ttggtatttt atgttcatac
     4441 gagattttgg agccatatca tttttagtta agttaaattc attatcatct acagttccat
     4501 taccatctaa atcttgtgtt ttaaatactc ctctttctga gtaaactaaa tccattattt
     4561 gttcccatgc aagcatacta ttatataatc tatctacttg tgtatttaaa tcagaatttc
     4621 cttttgatat ctcttgatat actgctgttg caggaacatt taatgttact ttatcacttt
     4681 caatatctgt agtgtttaac acaccagttt tttctgaata tccatatgaa ttaccaacat
     4741 ttgttcctgg ataatatggt tctcctttta cagcatcttt atatttagct aattcttcta
     4801 tatatgcttt tatctttttc ttagattcag aaactcccgc aggactctta tcatttattg
     4861 ttccatttag attaagatgt ggtatcttag tagctcctgc tacacgtact tttatatctc
     4921 ctccacttgg agtatcttca gcgtatctta tgtataaact tcctccacat tctatatcta
     4981 aactatgaag tttgcttaat tgaattacat tttttccagt gttaagattt atttctccac
     5041 tcttatatgc accagattct ccatagtact gtgtatatat caacttagga gtttttccac
     5101 ttttacttcc aacatatact gtgatttcat ctcctgctct agcagctact cctaatgcct
     5161 gccagtcatt ttgcatacct aaattattgg aatttacagt atactgtccg tttttatctt
     5221 tatattttcc taatttattt atttcttgat ttacagtagt tacatcagta agtgtggctc
     5281 tatcattata taagtcttga gctaatttta attctctctc aataactgtc ttatatggat
     5341 gatattctcc actagcttta tctggagtat ttaatctatc ttttaattca gttatttttt
     5401 cttgagttac atcttctttt aattcaactt gtaggccatc cataaataga tttcttacat
     5461 catcttctat actgtcatac tcataaaact taagttctga tatattagac ttatatgaat
     5521 aggatgaaca aacacttact tgtatcttgg tagcagttat tggttctttt aatctaacca
     5581 ttacatactt attattattt acagggtctg tctttgtttc aaaaggaaca tcccccagat
     5641 tgtgtaattg accattttca tcccaatatc gtacctcaaa gtcatttatt gtcttatcag
     5701 aaacatcaaa acgaggtgta gccataaaag tatccatagt ataagattta tcaaaagtta
     5761 ttattggtcc tctattcgtt ccctcagctg cattatatgt tgggaatgtc caatgtgttg
     5821 taaagtcccc atctactaca ttgaatttat caaatggatt tttatcacta tattcacttt
     5881 tgtcatatcc atctggataa acaacatcta caatgtgatt tgttggtatt ccaaatccac
     5941 cattttcttt aggagtatta attaatctat agttaggtgt tgttggtgca tccatgcttt
     6001 cagttgtacc tatatatgtt ttagacattc cactagttcc caagtggtta gtagctgtca
     6061 ttctgacttc atatgttgta ttatccttaa gaccttctat ctttgaactt gtcccacgaa
     6121 taagcttatc attgtatttt atccaagacc cattagagcc tttttcttta tagtacaagt
     6181 caaaatcttt agcttgttta tgatttttcc agcttacatc aagagattta aatttacctt
     6241 taacatttat accttctggt ggttctggtt ttgtgtctgg aacaacttga aattctaata
     6301 tactatctgg gctaaattgt ataggtttat agtttgaatc tacattttct ggtactccgt
     6361 ctaagttttc tggtgttgca tctttataac cactagacca atctccattt aaagattgta
     6421 tgctaactct atatagtcca tatgcttcta tatctctaac ttctattgta tttttactag
     6481 tttgtaattt tttagtagat aatacttttc ccttactatc aattttgtct agtataagtt
     6541 cgtatgctga tacatttact tcattattcc aataaaaaga tactttttca tttccaacag
     6601 cagtgctagt cgatatttta tttattttag gtatgttcat ctctggcttt ggtatttctt
     6661 tatatacatt gtttaaaaat tcaacttttg atatttctgc aagatttcta tcgtttgctg
     6721 ttgtagctgt tatttttata gttatctttt ttactgctac ttgtttacca agatttatta
     6781 ctatatcact attttgaaca tctgcatatg tttctaatgc tgacctacta tatgtagatg
     6841 ttgtagagtt atcatcttta ctgatattac cctcttcttt tggagtatct tcatctgaat
     6901 taggtttatc cattgatgaa ttttctttat cattattttt atcttgattg gtattagagt
     6961 ttgcttcttg tttatcgcta ttttcctgtt gtttatcatt attttcatct ttattatttg
     7021 agttattatc agtctggttt atattttcat ctttattatt tacttcttct tgatttactt
     7081 cttcttgttc gttatttact tcctcttgtt gctcctcatc ttttaagttt ttcatatatt
     7141 ctttgttttc ataaggaatc tgttgaacat taccatcttt gtcagtaacc tcaactattc
     7201 cttttgctgg agcagtacct tctggagctt ttatattgat gacttccata ttaagtgctt
     7261 ctgataaagc tatatccatt actgctggtt tttcctcaga tatttcatca ttttttgaag
     7321 atattgtaac tccattattt tctccactaa ataaattttc tacattacca ttaatatcta
     7381 catcactatt tccttttaat tcaaccttac ttgtatctac tataggattg gtagatacta
     7441 tactttctag gctttttgca tcttcacttt gtcctaaatt tttctgtaca taatctaagt
     7501 caacaatatc tacctttcca tctctattta aatcaaattc tcttttattt gaatctatat
     7561 tctctaaaac cttattataa tcactcttat ctactttacc gtctccattt acatcaccca
     7621 ttaagaacaa ttcataattt aaaggtttat ttttattatc tacacttgct ctattgctta
     7681 ataagactct ttgtgaataa cttgatatat caatgtcttt aatttctttg ttcttaaatc
     7741 catctccaga gacttctaca ttgtattttc caacaggtaa attattaaat acaatattat
     7801 aatatgatat attctcatct ttatttttta tttcacttct acttgcagat aattttttta
     7861 tagtatattc tatattatct gtcccaacat ttactgttcc tgaatctttt tctccaagct
     7921 ttattattcc actattgtta ctatcatctt ttaatcgtaa ctgcatatta gtcatacttt
     7981 ctgactcgct attttttatt ggcagtgaaa aatttaaatc tacttcaaga ctccccctta
     8041 acttttctgt aactgatttt tcaactgccc ttagcttccc ctctaatata ggttctgcaa
     8101 aaacgtttag tggtgcacta cacatagata ctgctagtac acaagatatt gcctttttca
     8161 tgaaataaca atcctccctt aataaatatc taatttttca tgaatttaaa tactataagt
     8221 ttcagacaat aaaaaaagac actcaaagag aatcttaatt ataaaataaa aagactttac
     8281 tctttttagc aactggctga tatggcttta aaagctttaa tccctatagt tttgcgtcat
     8341 tagatttccc taattttgcc atttaatttt cactactttc ttacccattt tatactatat
     8401 ttaaactttt taatataatt tttttagatt aaattataaa aaaataacat ttatattaat
     8461 ttattaatat aaatgctatt ttccattata atttaattat ttatatagct tccttacaag
     8521 cataattttg tgtatatttt cttattcttt atatcttcca taaaattcat gtgtagcata
     8581 tagattctta tctcctgtta tagctatacc aaatcccatt atttcatacc ctttatgcaa
     8641 catattagtt ccttcttctg gatttgtctt ccactcatta aaaataaagt ttcctatatc
     8701 atctgcatct ctatttgtat atacaggttt atctggaatt ttatatttaa ttatattttc
     8761 accttgaact gctatatatc caggtttaat ctcactccaa tctaagtatt tcaaaaaagt
     8821 agaatatgaa ttttttccat ttatcgtatg agataaagtc ttcttatttg ccattaaatt
     8881 agaccaatca tttgctagac cctctaatct gcttgctaca gataacgctc ttactccatt
     8941 ctcttttcta tactgctttg taagagcata tactttaccc tttattctct ctttaaatgc
     9001 tctatcattt ggattgtaat ataaaccttg ttgatttggt gaagcttgat ttatacattg
     9061 cttgataaca ttctgattta tgcctcctag tacagtcaat ttgtctgcac cacttaaaat
     9121 actcttgtta ctgccatttt caactaaaac tacaggagca ttttttgcta ttgtagaggc
     9181 agccaaagcg tctgttaatt gtaagccttt acttagataa aattctttac tatttttata
     9241 aaactcttgt ataactatct tattagtttc aaatctatct gtgccaccca atctaataga
     9301 atttgtgctc ttaactaatt cgtcgcttat tatttcacta gaaccaagcg cataactctt
     9361 taagcctgtc gtgtcaaatc ccacactatt tccatctgta agtattatag gtactccatc
     9421 tctagcagct actggtgatg cacttatcgc atctgcttct cctgtaaatc cattggctat
     9481 aattacttta tctattggct taatttcttt gattttttca gctattttta tacttgtatc
     9541 aaatctattt tcaccaaata ttctttgtac aaccttcttt ttggatagta actgattctc
     9601 aacattttta cttattgttg attctgaacc tattatatac acagtctttg ctttgtttaa
     9661 tctttccata gttgtgtttg gaatctggtt ttgttttgtc atcaaaatag gcgcatttaa
     9721 ggctccagct aaaccacttg cacttagacc atctgctaag ctattatctg agttgattag
     9781 tattgctgta ttgtagtttt gcttatctgc tatttttgct gctgtctcat atctgtcatt
     9841 cccttttatt tgctcaactg atgtcaacgc atttaccata ggagtattta ccattactaa
     9901 aaatagtgta agtcctatag ctaatatctt tttactattc ttcatattat tttctacccc
     9961 caatgtactt ttcatatttt ttattagtac tcatacttat ttttaatcaa aagttacaat
    10021 tttgttacca tattttatat atgataccat agttatccat acaactcaaa caatatacct
    10081 aaagttttta ttctctataa attcaaaaca ttataattta ttttatttaa ttacgttata
    10141 agttcaaaat aatttatgtt agtaatatgt ttaatcatta taagaagtat ctttttataa
    10201 taatttttgc aatatgaatt tctcaacatt ataatcaaaa aataaaataa ggtatctcaa
    10261 tctaattcta aatgagacac cttattttta ttattttatc ttgttatttg tatattttat
    10321 aatatctaca ctatgtctta catattaacg cattgtataa tcatatatta tctaaataaa
    10381 cttttaatat taattcctat atcttattag ttcttagtaa ataagtttat tttttctgct
    10441 attttttcaa tagcgttttt agctattcct tcacctattt gtactaactt agtagcattc
    10501 ttaagtctta actcaactac ttcttcttgt gatttagaaa gttcttctgt agttaatact
    10561 attggagcct ttgaactagc tgcaagtggt gctgctgtaa gtgcatcaac taattgtcca
    10621 tttccaccta cataaccatc ttttgctaca tatgctttag ttgcacctgc atagtattta
    10681 ttgattactt tagcattagt atcttttcta ttttctccag ctaatctttc aactttatta
    10741 tttaaatcta tatcaactaa tttgtcttca acttcttttg atacagaatt ttctccaccc
    10801 actatttcta attcttttcc ttttaataat ttttcagcat ttgcatctaa ttcgttacct
    10861 gtaactatta ttggagcacc aaattgagca gctctagctg atatactcat agcatcagct
    10921 tctccatttc cacctactac aaaagcagtc ttaaatgtat cttttaattg ttttgctatt
    10981 tctaaagaag tttcaaatct atcttctcca gaaactctct caactttact tacacctaat
    11041 ttagaaagtt tttcttcagt ttcttttgat actttagatt ctccacctac tatatatatt
    11101 ttcttagaag atagattact tccaagtact cttaatattt cagcttctat ttctgatggt
    11161 agtgaatctt ttttagataa taatattggt gcattttttt gagaggcaag tggtgctgat
    11221 gctaatccat caactatagc atcttctcca actaaaatta tagcttctgc ttctccatct
    11281 ttaaatctct ctttactaac ttctactgct gttgtaaatc ttgaatcacc agataaaact
    11341 tctactttta attcttttcc atcttttaca tcagttaata ctttgtgtaa gtctctaact
    11401 aacttatggt ttttacttga aacacttatt gtttctggct tttcatttgc cttgcttaaa
    11461 actatatcaa agcttgattt ttctggttta tctttatcat cactatcttc ttgttcttga
    11521 gtaaggattg cttcattttc aaatgttaaa gcatatccat ttttaacttt attaactatt
    11581 tctcttccta atgtagttaa tctaattcca tcaaataatt cttctgctga tatgtcttgt
    11641 aaatctgcgt ttttaactgt tatcgtatgt aattcttttg aatctatgtc actataactc
    11701 ttttcataac cttcaaaagt accttcttct gatgttatca cttttgagaa atctaaatgt
    11761 tggtctccta catttaatgt aataacattt gctgaatctt ttgctctttt tacttcaact
    11821 gtatttgtag attttatagt tgctgttaac ttgttatcag aatcttctgc taaagttgca
    11881 ttgtaagcat taactgcatc tactatttct tgagcagttt tgtagttttc aattttatag
    11941 tcaactactt ttccacctaa cacttgatgc cctttatctt gaatagtaac ttttaaactt
    12001 tgcccttctg ttaaagagtt tattttattt tgtaattgag ttgcattagt taagtttaca
    12061 ttgtctactt ttatatcgta aactctatca tttacatttg ctcctgcttt tgtatcttca
    12121 aacttaactt ctaatgcttt tctaacctct tcgattagtt tagcagaatc tttcgcactt
    12181 actgtgtaat ttttatcaac attttctgtc acggtattgt ctgcgaatat tggtgctgct
    12241 gcactcataa ctgtagcacc tgccattact attgccatac ttctcttttt gttgcttaac
    12301 atatttatgt cctccgaata ttatttttta ttttttatat tcgtatttcc cacacataaa
    12361 taatattata tataatatta tttttcaata tattttatgt gtttacataa tttactaaca
    12421 caaacactaa taatatcttc ttgaaaccct tccccctact taggctctct ataattatgc
    12481 tcaaagatag aaatcctatt ttatagtgat tttctactaa acaatcatat actaacaaaa
    12541 gtattcaaac tccattttta tatactacta tgcactaatt tttgctttac tcttattact
    12601 taaatatata tcactaaatt aatatctatc tccatatatt aaaatatata acatattata
    12661 aaaatcatca taaattagag tttaaaatta aaaattattc taatacatgt aattgataaa
    12721 aaataaaata taagagtagc tactttaaaa taaatgctct tacatgatta gatatttttg
    12781 cataattaaa cgataagaaa tcaaatttac ttctacataa atagaatata tatttttata
    12841 taataattga aaataaaaga ggatacctca taatgataaa agaatcactt tgagataccc
    12901 tcttacaaac aactaaatca ataattttct atctttcaca ctcttgattt gcaactgtac
    12961 aagaagtttt acaagctgat tgacaagaag tttgacactc tccacatcca ccttttgcag
    13021 cactttgctt taaagtagct tgagataaag tttttacatg tttattttcc attttcaacg
    13081 cctccatttc taattgtttc ctattgatta tatcataaaa ctactttaat ataaactcta
    13141 tttaaagttg acacccaata cgcctccaat gcctcctgct gctgatatta gcaataactt
    13201 ataaatcatc cctatttcaa atacaaactt ttcttgtgct aaaaacacag tcaaaaaaat
    13261 acatactatg tacaaaagtc ctactaaaag cccatacata agtccttttt ctttcatgtg
    13321 ttttgaagtg taaaaccctc ctatagctgc agaaatagta gttataaatg atgagaccat
    13381 agttatatta tctccaccaa tatctgtaaa tgtcaaaaat aagttataca ctaataaaac
    13441 agccaaagtt attatatatg catatcctag acctttaaaa atataattag attttcccat
    13501 aaaaaaacct cctctcaaat taatatatat tcgagaaaag gcttttaaat tactaattta
    13561 gtcttcttct atttcttctt ttttagattt aacagttcct atagcccatt tttcaaatgg
    13621 aactttagtt ctatttggtc ctaattctaa tattacaaca tcatcttcaa ctttagctac
    13681 attagctact attccaccta ttgtaatgac cttgtctcct acacttaggc tgcttctcat
    13741 ttcttttaat tgtttgtctt ttttcttttg aggtctaatc attaggaaat aaaagattgc
    13801 gattactact acccatagac ctatactcat aattatttgt tgctgtggca ttaatttttc
    13861 actcctttgt aaattatact cttttgtaaa tcataataaa tgtttatata acaatttact
    13921 ttctctatct ttgagtacta ttgaatatta tatattaaaa tgattataat taatagtgca
    13981 aaacctatat ttcatatcca tattttgcaa aaaattcatt tctaaaatca agtaatctat
    14041 cttccattat cgcttgtctt atttgtttca ttaaatttaa taaaaagtga agattatgag
    14101 tagttattaa tcttgcaccc agtatttcat ttgcttttat aaggtgtctt atataagctc
    14161 ttgaatagtt tttacaagca taacaatcac attcagggtc aagtggagta aagtcttcag
    14221 cataagttgc atttctaact actactttac cttggctagt cattgcagtt ccatttctag
    14281 cgatacgagt aggtagaaca caatcaaaca tgtctactcc tcttataaca ccttctacta
    14341 agtcatctgg actaccaact cccattaggt atcttggttt atcctttggc ataagtggag
    14401 ttgtatgctc taacacgtca tacataagtg gttttggttc tccaacacta agtccaccaa
    14461 tagcatatcc tggtaaatca atattagtta tttctttcgc tgattgttcc cttaagtctt
    14521 tatacatccc accttgtatt ataccaaata aagcttgctt gtcggtatta ttatgggcat
    14581 ctttacatct ttttaaccat cttgttgttc tttctaaaga attttttaca tattctctat
    14641 ctgatgggta tggtgcacac tcatcaaatg ccatcattat gtctgaaccc aatgcatttt
    14701 gtatttccat agctttttct ggagttagaa aatgttttga accatctaag tgtgacctaa
    14761 attctacacc ttcttctttt atctttctta aaggccctag actaaatacc tggaaccctc
    14821 cactatctgt aagtattggt ttatcccaat tcataaactt atgaagacca ccagctcttt
    14881 ttataagttc atgacctggt ctcatatata aatggtatgt gttactaagt attatttgtg
    14941 aaccaatctc ttttaattct tctggtgtca tagattttac agttgcttga gttccaactg
    15001 gcatgaatat tggtgtttca ataatcccat gtggagtgtg taatcttcct aaccttgcac
    15061 cactctgctt acaagtcttt atcaattcgt atcttactgc gtacataaag ttctccttaa
    15121 tcatatttat ttctcattat ttcttaagat atatttgtaa taagctattc atataattta
    15181 ttgtctaaat agatttatct tcgtcttctt tgttctgttg ttccttaatt gcttcttcat
    15241 catgaatact cattatatta agagcactct tgtcttttgt tactaaaaac ttttttatag
    15301 caaatagtcc tgctatagct ataactccta ttaaaacatc tatcatacct tcatttttag
    15361 gcagtagcaa catttttcta gcaattgcgt aaagtaatgc ctcaataaat gctcctggta
    15421 tatgaagaga tagcattact acaagttcaa cacctattac taaaagtaat atttgtgcta
    15481 aaaaatcatt aagctgactg tattgtactg gattttgaaa atcaactata tatgctcccc
    15541 acataagtct tagaacatct atagttccta aaaatactgc tattagaact actactgcta
    15601 aaacacgttc aaatataaaa gcaatcttta atatagggtt attctttttt tgtttttgta
    15661 aactatgcat ccattacctc cctactttat aatcatagca tctccaaaac tgaagaatct
    15721 atatctttct tttactgcct cattatatgc atttaaaaca ttatcctttc cacaaatagc
    15781 acttactaac attattagtg tagactctgg taagtgaaaa ttagtaataa gtttatctaa
    15841 aactttaaat ttataacctg gatatataaa tatttctgtc catccactag tttctttcat
    15901 ctttccatac tcattacaag ctgattctat agttctacaa ctggtagtac caacacatat
    15961 tacatttttt ccattttcct ttgctctatt tattttgtca gctgcttctt ggctaaccat
    16021 ataatattct gaatgcatct tgtgatttaa aacatcctct acttttactg gtctaaaagt
    16081 tcctaagcca acatgtagag ttacaaaggc tatatctaca cctttttctt ttattttatt
    16141 taacaattct tctgtaaaat gtaatcctgc tgtaggtgct gctgctgaac cattatgctt
    16201 tgaataaaca gtctggtatc tttctttttc atctagtcta gcagtgatat atggaggtag
    16261 tggcatattt ccaagttcat ccaatatttc ttcaaaaatt ccatcataat ggaattttat
    16321 aattcttgaa ccttcatctg ataaatctac aacttctcca attagctttc catttccaaa
    16381 agaaaattta gtccctactt ttgctctttt tcctggtttt actaaagcct gccaagtatc
    16441 ttcctctgtt ctttttaata ataaaaactc tattttacct ccagtttcaa gcttctctcc
    16501 tattaatcta gctggtataa ctctagtatt atttaatact aagcaatctc ctggatttaa
    16561 atattctatt atatctctaa atactttatg ttcaatatta cctgtctctt tatctaatac
    16621 catcaatctt gagctagctc tatcttctat aggaacttga gctataagtt cctgtggtaa
    16681 gtcaaattta aaatcacttg ttttcaatgt taaacttcct ctcttaaaat ttctccagct
    16741 acaataatat caaaaaaaca acttattttc cacaatatta ccataatcta tcaagaaatt
    16801 gctattttta acagacacca tatccacaat atatagatat ttttccacga tatacatgtc
    16861 ttatccacaa tatatagata caatacacac aatttttatc gtgtttatcc acagtatcta
    16921 cagatataat taccattttg tggatatgtt tgaattttta gccattttag aaatatttta
    16981 atttaatgac tcttttattt tataaatact cgtataatct atttttattt ttcattagga
    17041 tatggaattt ttaagtgttc atatgctaat ggcatagcta ctcttcctct aggacctcta
    17101 tttataaatc ctaattgaag caaatatggc tcatatacat cttctattgt atttctatct
    17161 tctccgatag aagctgccaa tgtatctaaa cccactggtc ctcctctaaa tttttctatt
    17221 atagtcataa gaagcttctc atctacaaaa tcgagaccca aactatcgac tcctaaaagt
    17281 tccagagcat cttttgccac cttatcagtt atttttccat cagctctgac ttgtgcaaaa
    17341 tctctaactc tttttagtaa tctatttgct atccttggag tccctcttga acgttttgca
    17401 agttctaatg ctccattaga ctgtatctct gcatctaaga tgcttgaaga cctaagtact
    17461 atttttgaaa gttcatcaac tgtataatag tccaatttac aaataacacc aaatctatct
    17521 ctaagtggat ttgtaagcat accagctctt gtagtagctc caataagtgt aaatttagga
    17581 aggtcaagtc ttatgcttct agcagatggc cctttcccta ttattatatc taggcaaaaa
    17641 tcctccattg ctggatataa aacttcttca acacttctat tgattctatg aatctcatct
    17701 ataaataaaa catcattttc atttaaattc gtaagtatgg ctgccaaatc tccagctctt
    17761 tctattgcgg gtcctgacgt aattcttaaa tttaccccca tctcatttgc tattatactt
    17821 gctagtgtag tttttccaag acctggtggt ccatataaaa gcacatggtc taactgctca
    17881 tttctcgatt tagctgcttc tataaatata ctaagttgtt ctttggattt ttcttgaccc
    17941 aaataatctt ctaaagtttt tggtcttaaa ctattttcta tatctatatc ttccatcttc
    18001 attgtagaag ttattatcct attttcatct tcaaaacctt gcatttatac ccctcctctc
    18061 atacttttga aaatctactt tatatatttt caaatttaca taaactcctt aaatattgtt
    18121 ttaaaatcca aatattataa aatccaaata ctatttcaag gatttactca ttatatatgt
    18181 aagtgctttc tttataatag cttctgtatt cataccatct tttttacatt tttgaactgc
    18241 ctcttttgat tccgacaaag tatagcctaa agctacaagt gcatctacag actcatcttg
    18301 tgatatcaag gtaggttttt gtgataataa cgttggttca aactctatgt tatttttgtc
    18361 taccttatcc ttaagctcta agacgattct ttctgctgtc tttttaccaa caccaggagc
    18421 tttggataat tttccaatgt cttcacttaa aatatatgct cctaactgtg ctggtgatgc
    18481 aaatgatagt attgaaagcg ccactttagg tcctatttta gatacagatg ttagtaattc
    18541 aaacatctta agttcttccc tacttacaaa accacaaaga ctcatatcat cttctcttac
    18601 aattaattta gtatatattt ttgtggcttc tcccacttgt aaattcatta ttgtattgga
    18661 tgatacattt attttatatc ctatcccatt attttcaaca actatactgt ctatataaat
    18721 ttcttctact gtacctttta tataactata catacttacc acctatattc tttagagttt
    18781 tttcaagttt gtttgaatga gcatggcaaa tagccacagc taaagcatct gcaacatcat
    18841 ctggttttgg aacttttttt agacttaaaa atgaagttac catctgttga acttgtgcct
    18901 tatctgctct cccatatcca actacacctt gttttacttg aagtggagtg tattcatata
    18961 caggcttccc attatgagca catgcaagca tagtaacacc tctagcttgt gcaactgtta
    19021 tagctgtttt tacattcttg ttaaagaata attcttccat tccaacttca tctatattgt
    19081 aattctttac tattgtatca attcctttat acacaagttc caatcttctc gatatattca
    19141 tatgggcagg tgttgtaact gctccataat cgattacttt aaacttgcta tttttgtatt
    19201 caattatacc atatccaact atggctatac ctgggtcaat ccccaatatt atcatttttt
    19261 aaaattcccc ctaaaaataa aagaaatacc aaaatgtatt tctcataaaa acttatgttc
    19321 taaatcatta tatcataaat aatattaaca aatcataaaa tgctctatgt aaaatataaa
    19381 atcaaataaa aaagttatat caaagcaaga ttttacctca atataacttt tatatttatt
    19441 acaaataaat tattgtattt ttataggaat taaatgtgac tacaatttta atactaatct
    19501 atttaatatt aaattattta ttattaattt acttagtatt taacttactt agtatttaac
    19561 ttaaaataat attaatttac tattaacttg caaagtcttc taatcttgaa taggcctctt
    19621 cttcagtatc cataactaca ccttttttga tttcatcttg aactgtttta ggaagtgaat
    19681 ctactcttat ttttgttttt tctactaatg ttttacttcc gctatcatca tatttatata
    19741 gtgctatatt tccattatca ttttcaatag tatatttacc ttttatagta acatccattg
    19801 cttgtacatc tttattatca ctatatttag attctaccaa tatgatttca tttttatcca
    19861 tactctttat ttttttagtt ggatattctt ttttaaaata tgatactatt tctgacttcg
    19921 tcttatctaa taatgactct ggaactattc ctgccattgt aagtgttctc ttatagttag
    19981 tttcattttc gccttcactt tgaagccaaa cttcacaatt tttatttaat ccaaaagctt
    20041 cttttgactt ttcattcata ttcgcactag tacttacaac ttcaccagtt tgaactccat
    20101 tgtgttttaa tcttgtccct atataaactc cgctacttaa tacaaatact ccaaccagta
    20161 ttaaggcaga tattttccaa ggaaataatt cttttttctt cttttcaaac attgattacg
    20221 cctccttttt ctattatttt ctccaagctg taagttttta tacaatttag gtaaatttta
    20281 gaagaaaata tatttatatt tcaaattact ctaagaatag atattttcta aaaggtagtt
    20341 ttctcttaat ggttatattc ttgtaattat atggacataa tttataataa ataatcgttt
    20401 aggaggtaat aactttggaa agaataaact gtaatgtaat taactgttca cataacaaca
    20461 gccatatatg ttatgcagat aaaattttta taaatggtga aaaagctcac aatgacagac
    20521 atacttgttg tagttcattt ttagatgaat ctatttatag taatttatct agcaacgtta
    20581 ataatagaaa ttgtgaggaa ttagtttgta ctgtaaaaaa ttgtaaatac aacaaagatg
    20641 gtagcttatg tacacttgat aatatattgg ttgctcctga aactgataga gtaaaccttt
    20701 atattgaaac atgttgctct agtttcgaat gtaaatagtc atagtataaa tataagggtg
    20761 tcaaatgtaa ttaaactaac atagtgtaga gtatctacaa ctattagttt gattatactt
    20821 gacaccctta tttattttaa ataaaatttc attattataa ccatgtttat aatgttctaa
    20881 ctgtaattaa atttgataaa tttctataaa ttggtataat caatatgtaa attgaattta
    20941 gtaaattaat caaaaaaata aaatatcgag tatatagtgt tatactaact agaaatgagg
    21001 tgcttatgag tacattttat attcaatttc tttattttgt aatttatagc tttataggat
    21061 ggtgttgtga aactacatat tgttcagttt tacaaaaaca atttgtaaat agaggttttt
    21121 taaatggtcc attttgccct atatatggat ttggagcatt agcaatagtt gctaccttga
    21181 ctccttttgt acacaatatt cctttgttat ttttgtttag tataattata actagcataa
    21241 tggagtattg tacgagcttt attttagaaa aaatatttaa tatgacttgg tgggattatt
    21301 ctaaacataa atttaatatt catggaagag tttgtctgga aaactcactt atgtttggtg
    21361 tattatcatt gattgtaatg cttatagtac atcctattgt ggttgacttt attaactcta
    21421 tctccaaaaa tgtgctattt atatttgcta tatctataga aatttatttt gttttagatt
    21481 tagttatcac agtccataca attttacaat taaatggtaa gttaaaacaa atcaatctta
    21541 taattaagga attaaaagat aagaaagaat actacaaact tattacacaa gaaactatag
    21601 aaaacaaact tgaatcttta gttgaaaata gactggatac tttggaagat agatttgaaa
    21661 tacttaagga aaaagaaaac tatcgctatg ctaaaaatcg tattgatgag ctaaaaaata
    21721 aacttaatac tttgctgtct aaccacaaat tacttcatag acgtataatc aaagcattcc
    21781 ccaacataag ctctaataag catacagata ttttaaataa aatcaaagaa aatattaaat
    21841 caattaaaaa agataaataa taaaataaac acataagtta aataataaaa atactaatac
    21901 ataagattaa ggtttagaat attttttgta aaatactcta aaccctaatt aatatactat
    21961 tatttttaat cttaatttat aattttagtt tattttactt ttaacacagt aaatttaagc
    22021 gtttaaactt caatacacta atatactacc ccatcaatat ctcaactgat tctctaagtg
    22081 tgctaatctc tcccacattt ccagggaaaa ttatatatgg catattagga aatttgcttt
    22141 ctttgcctgt catccatact ggtataccag gttttatctg tcccattact gtagcttttt
    22201 ttactcttag tgctttagta ccaacatcac tagaagtaat tccaccctta gctataataa
    22261 aatttggcct tacattaagc ataccaatta tactggtaac tgcatctgat atttctacag
    22321 aaatcataag ctgtttatct ttatcatttg tatccaagtc aaaacgctct cttctagtat
    22381 aaacagcaac agttttaccc ctgcttattt tttcctctac aacttttata actcttttta
    22441 cctcattttt tagaccattt tcttgaagaa ctaaatgttg attaaattct ataaattcta
    22501 ttggatattt acaattttta agttcttcta actgttgggt agtcttattc acatgtgaac
    22561 caacaagtat aattcctcca ttggtattac cttttgatac taattcatcc ttagtaagca
    22621 gttccttatc actcactccg ccaagtactt ttgttatggc agcagcgctt ctaaacataa
    22681 attccttacc actatttact gctcgtataa acgcaattgt aaataccttt acatctacat
    22741 agtcggttgc atttacaata attttattga atccttcagc ttttttaagt ttttctgtta
    22801 tagcttcaat atttagctct ctaagttctt ttgacgaaat atatatcata tcactagatt
    22861 tgtactttcc ttttgtcttt tcttcacacc actcacctat atcagatgat ttatatccaa
    22921 aagatttatc ttttgcaaat tcagtcattc ctgctggtac aagtatttct ccttctttaa
    22981 cataatgtac attatctata gtaaacctac caccttcttt aaaaaatggc attattattt
    23041 caccctgaaa taacttcccc gataatctct ctacttcgct ttttaataaa ttagtttcaa
    23101 gaggataatg tcctctcata gtagaatcac tacgacttat gattataaaa tccttattaa
    23161 ctttctttga tacatccaca atattctttg aaatttcttt atgtgctttt gtagtttgag
    23221 caacagtaaa tccccttgaa ttggtaagta taaaaaacat actatttttc tcattaaaac
    23281 cttgttccat actatcctta tcccaatcag tataaacaga aatatcatga actgtttgta
    23341 cacctgttgg gtcatcatct aatacaacaa tttttctatc taaatgctta agctcatctg
    23401 caagaagttt atttaatctg tcctcatcta cttgtttaaa agtattaagt atatctatgc
    23461 taattggttt tacttctctc atttaaaacg cccctttatg taatttgtat agtctaaatg
    23521 taatttatat gatataaaca atagtttgtt atttatagac ctggaagtgg tataattcca
    23581 tttataacac taagcaataa aaccattata aatgctacaa ctccagcaat ggttcctcca
    23641 attgtccaag agtttaaagt atctttaact gataatccac taaatcttga aagtacccag
    23701 aatccagaat catttggaag ggagaaacca actcctccag cacatatagc aagccctact
    23761 aaaactggtg acattccagt tgccaaaatt gtaggtccta aaattgatga tgttgttact
    23821 aaggctacag tagcagaacc ttgagaaatt ctaagcaagg cacttaataa gaatccaagc
    23881 actacaactg atatactaag accagacata gtatcaacta agaagttacc tataccactt
    23941 gcatttataa cactgccaaa cgcaccacct gaacctgtta tcaataatat tagccctgct
    24001 gaatcagcag cttcaataac tacatctccc ataggtttat ttatgtattt ctttaaaaat
    24061 cctattgcca ccaatattcc aagaagtatt gctatgtttt tatctcctat aaatgcaagt
    24121 actccactca ttgtagaacc tttttctaag aacatactaa atacatttga tataagtata
    24181 agaagtattg ggaacaataa agaaaacata gaaattccaa aacctggtac tttagaagaa
    24241 tcatcatctt caatttttat gctatctttt ccctgctcta aatttttttc tctatcattc
    24301 tcatcaatag cataagctgg atatttttca aaacgttttc cataaatcca tccacctata
    24361 agagctgctg gaaaagcaac aattatagaa taaagtataa aactacctac atttgcattt
    24421 acatttgctg caactgctaa aggtcctggt gtaggtatta caagtgcatg acctactata
    24481 gtcccaactc ctaatgcaca tacaaaaacc attagagatt tcttagttac tctcgaaaca
    24541 tacttaatta taggcatcat tataacaaaa gctgcatcca taaaaactgg aattgaaatt
    24601 aaaaaacctg ctgtagttac tgctaaagca ctgttttttt ctccagtctt agctaacaac
    24661 ccctttgcaa tagattctgt tgctcttgat tctgaaagaa tatttccaaa tattattcca
    24721 agtccaatta caatacctat actacctaat gtatttccaa atccagtcga tattgtggtt
    24781 gatatttcat ttataggcat tctaaccata aagcctgtaa ttatagatgt tatcagtaat
    24841 gctaaaaacg gatttacttt aaatttaata atcgatacaa gtaaaattcc tatagatata
    24901 acaaatgcta tcaaaagtcc atatcctgta atcataatct ttaccccttt atcattctac
    24961 tttattattt atcaatttta tttttctttt actatgataa cttaacctcg ctgtatttat
    25021 ataatctaat cctaataata tgtatttaat tatattgata tatttaaata ttaattatcg
    25081 tgttttttta tttcaacacc tgccaacttt tcaaaatact gtgctattcc actatggtca
    25141 tcttccaaat gtccatgtac ttttaatgtc tgcataattt ctaataattg acttgtcata
    25201 ggtagcggta catctaaatt gtgtgctgtt tgcattacat tcttaatatc tttcaaatta
    25261 atagatattt ttccacctgg tttaaaatca cgattcataa tcattggtat cttcgcatcc
    25321 aatattgtac taccagctag gcctcctctt attgccttat atactttttc tgggtctgca
    25381 cctgcttttg ctgcaagtac aaatgcctca gaaactgctg ctatcgtcaa attaactatt
    25441 acttggtttg taagttttgt aacacttcca ctaccattat ctccaattaa aatagatgag
    25501 cttcccatta tattaaaaaa tggctctact ttcttataaa tttcttcctt accaccagcc
    25561 ataaaagcaa gtgtaccatc aatcgctttt ggctcccctc cacttactgg tgagtctata
    25621 aattctaagc ccatatcttt taatttattt gcacacaata ttgattctgt aggagtaact
    25681 gaactcatat cgactactat acttccttct tttaaagttt ttgccaatcc atcaatccca
    25741 aacaatatat cctgcacaat agttccattt ggaaggattg taaatacaat atcacattct
    25801 tgacccattt ctaaaacact tgcgtatttt gctccacatg atataagttc atccacagca
    25861 gatttattaa tgtcatacac caaaagatta catccatctt ttaataaatt ttttgccatt
    25921 ggctttccca ttattcctaa tccaataaat cccaatttca ttttataacc ctccatatct
    25981 tcacattttt gaaaacgttt tttatgtttt agagtatact acataccagt ataccggtca
    26041 atataaaata taaaagtatt tattttaaca ataaatagta atttttaaag tcatttatta
    26101 tatttagtca tcctatataa gttttaatac atactattgt tttcaaatat aatatactac
    26161 ttttacagtt ttgtatttta actaatttca attagtttta attaatctgc ctttaatgtt
    26221 aatcatgctt cacacttcaa taatttttta tattttttaa tcttatctgc aatttaaatt
    26281 gcaaaagatt gaccttattt tcactatgtt gtatgcttaa aattatataa gtttttgtat
    26341 ttttatattg tttagggggg tattattaag tatggctaca tttaaaagat tacaagaaca
    26401 agcatatgac tacttaaaag aattgatact atctggaaaa atgatagaaa atgaaatata
    26461 ttctgaaaca aaacttgcta gtgaaatagg tatttctaga acacctataa gagatgctct
    26521 tcaaagactt agtcaagatg gatttataga tataatccca agcaaaggat ttagaatcca
    26581 tcaaattaca gcaaatgaaa ttgtagagat attccaaata cgctctgcaa tagaaggatt
    26641 ttgtactttt ttaattacaa gtcaatacaa agaagctaga gcagtagaaa ctataagtaa
    26701 attaaaacat cttttagata aacaaaaaga tattttatta ggtgataaaa atttaaactc
    26761 atttgcagaa tatgatacat tatttcacac aactatagtt tcatatgctc aaaatactga
    26821 atttgacaaa atgtttaaca attatatgta cagaataaaa aaattagctc tcgattcatt
    26881 gtcacatgaa ggaaggcttg aaacaacatt aaaagagcac acagatattt ttaataatat
    26941 tgcaaatgga tgtattgaag atatttacag aactactctc attcatatgg aaactcctaa
    27001 atacattaat ttagaagatt tttgtaacaa taagttattt tagcattata ccttcattta
    27061 ttaaaaatat atctatctca aattaatttc ctaatttata aaaaatattc tataattata
    27121 ttgtaactaa tacttattct aaggagtgat ttttttgaac gaagatgttg taactaatga
    27181 aactattgac aaagattatg ctattgaaga agttcgtatg gcatgcaaac attttggtga
    27241 tttatatttt tatttttcca aagtgttatt tgaagagttt ggtgaagata aaactagcga
    27301 aatattaaga aaagttttat ttgaaaggtc tgaagaaaga gctattgcta tgagagagag
    27361 agctcatgaa aatggagatg aattaatcgc agacaatatt attagcacaa ctgatgttcc
    27421 atttctagga tgggttcctg aatttgaaga gttacattgc ccctatggag cttcctggct
    27481 tttaagattt gaggaaaatc cttggtttaa aaaatttgct tctctttact gtgatgttac
    27541 agatactact gtagctgaag tatttacagg agatacttcc cataaaataa caaaaaacat
    27601 tctatgggga gataaaagtt gtgaaagaat atatttccat gatgacaagg tatctgaagg
    27661 taaatatact tatggaaata aaaactgtaa ataaataatt taaattttct atattcatat
    27721 actata
//
LOCUS       DAELNY010000036        27451 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.036, whole genome shotgun sequence.
ACCESSION   DAELNY010000036 DAELNY010000000
VERSION     DAELNY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.036"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            101..643
                     /locus_tag="KPE55_001316"
     CDS             101..643
                     /locus_tag="KPE55_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492409.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            722..1189
                     /locus_tag="KPE55_001317"
     CDS             722..1189
                     /locus_tag="KPE55_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG0492410.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            1898..3034
                     /locus_tag="KPE55_001318"
     CDS             1898..3034
                     /locus_tag="KPE55_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG0492411.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            3093..3875
                     /locus_tag="KPE55_001319"
     CDS             3093..3875
                     /locus_tag="KPE55_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0492412.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            3894..4904
                     /locus_tag="KPE55_001320"
     CDS             3894..4904
                     /locus_tag="KPE55_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0492413.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            4923..5720
                     /locus_tag="KPE55_001321"
     CDS             4923..5720
                     /locus_tag="KPE55_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0492414.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            5818..6663
                     /locus_tag="KPE55_001322"
     CDS             5818..6663
                     /locus_tag="KPE55_001322"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG0492415.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            6750..7925
                     /locus_tag="KPE55_001323"
     CDS             6750..7925
                     /locus_tag="KPE55_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0492416.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            8120..9022
                     /locus_tag="KPE55_001324"
     CDS             8120..9022
                     /locus_tag="KPE55_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0492417.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            9036..10040
                     /locus_tag="KPE55_001325"
     CDS             9036..10040
                     /locus_tag="KPE55_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0492418.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(10184..10411)
                     /gene="acpP"
                     /locus_tag="KPE55_001326"
     CDS             complement(10184..10411)
                     /gene="acpP"
                     /locus_tag="KPE55_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0492419.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            10583..11008
                     /locus_tag="KPE55_001327"
     CDS             10583..11008
                     /locus_tag="KPE55_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492420.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            11056..11262
                     /locus_tag="KPE55_001328"
     CDS             11056..11262
                     /locus_tag="KPE55_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492421.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            11444..11623
                     /locus_tag="KPE55_001329"
     CDS             11444..11623
                     /locus_tag="KPE55_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492422.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            11657..11857
                     /locus_tag="KPE55_001330"
     CDS             11657..11857
                     /locus_tag="KPE55_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492423.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            12110..13126
                     /gene="ccpA"
                     /locus_tag="KPE55_001331"
     CDS             12110..13126
                     /gene="ccpA"
                     /locus_tag="KPE55_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG0492424.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(13249..13647)
                     /locus_tag="KPE55_001332"
     CDS             complement(13249..13647)
                     /locus_tag="KPE55_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492425.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            13859..14764
                     /locus_tag="KPE55_001333"
     CDS             13859..14764
                     /locus_tag="KPE55_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492426.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            14879..15059
                     /gene="ssrS"
                     /locus_tag="KPE55_001334"
     ncRNA           14879..15059
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPE55_001334"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            15242..16459
                     /gene="cdeC"
                     /locus_tag="KPE55_001335"
     CDS             15242..16459
                     /gene="cdeC"
                     /locus_tag="KPE55_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG0492427.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(16559..17881)
                     /locus_tag="KPE55_001336"
     CDS             complement(16559..17881)
                     /locus_tag="KPE55_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0492428.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(17932..18726)
                     /locus_tag="KPE55_001337"
     CDS             complement(17932..18726)
                     /locus_tag="KPE55_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0492429.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEQMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTVLDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            18836..20281
                     /locus_tag="KPE55_001338"
     CDS             18836..20281
                     /locus_tag="KPE55_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0492430.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            20310..20519
                     /locus_tag="KPE55_001339"
     CDS             20310..20519
                     /locus_tag="KPE55_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG0492431.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            20539..21933
                     /locus_tag="KPE55_001340"
     CDS             20539..21933
                     /locus_tag="KPE55_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0492432.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            22173..22925
                     /locus_tag="KPE55_001341"
     CDS             22173..22925
                     /locus_tag="KPE55_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0492433.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            22951..23364
                     /locus_tag="KPE55_001342"
     CDS             22951..23364
                     /locus_tag="KPE55_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0492434.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            23368..24111
                     /locus_tag="KPE55_001343"
     CDS             23368..24111
                     /locus_tag="KPE55_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG0492435.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            24111..24581
                     /locus_tag="KPE55_001344"
     CDS             24111..24581
                     /locus_tag="KPE55_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0492436.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            24598..25356
                     /locus_tag="KPE55_001345"
     CDS             24598..25356
                     /locus_tag="KPE55_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0492437.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            25340..26182
                     /locus_tag="KPE55_001346"
     CDS             25340..26182
                     /locus_tag="KPE55_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0492438.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(26342..27247)
                     /locus_tag="KPE55_001347"
     CDS             complement(26342..27247)
                     /locus_tag="KPE55_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0492439.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        10786 a         3022 c         4973 g         8670 t
ORIGIN      
        1 aatgtatttt tctattaaga tttcaaaaaa gaataagttt aattattaaa attttgatta
       61 atattatagc acaatagcac aaaatataag gagactaaaa atgaagaagt ttataaaagt
      121 ttcatttttt attttgggga tttttacact attcatagct ataaataaat tagtatatag
      181 agatatggta agtgtcaaag attatgaaat aaatcattta gaggaaattg tttttgattt
      241 tgatagcagg agaagtagag atgttggtac tattgtaaga tttaaaataa gcaataatag
      301 taaatatagt tataaattaa acagtgctaa aatgaagttt gaaaattgtg tggaaaataa
      361 aaagggaatg aataagtgtt ctgcatatgt aaatgtagat ttatttaatg aaaaaaattc
      421 tataactgaa agaactctga aatatggaat aaaaccaaat aatcaaggtt atgttgattt
      481 tgtaattcca aaaggactaa attttgatta taggtacttt aatgaatctg gtatgagtgt
      541 agagtataaa ggagaatata ttgtcaatat tccaatggca aaaggacttt atctagtagt
      601 tggaaaaaat aatgatacat ggaatgctaa tattatggaa taatttgaaa aataaatcaa
      661 ttatagatat aattaagaat aatatgaaat ttataaaagt tcagactagg aggggtattt
      721 aatgtcaata agtaatgtaa gagagtattt taaacaattt ggcaaggaag acagtatatt
      781 agaatttgaa caatctagtg ctactgtgga acttgcagct gaagcagctg gggttattcc
      841 agcaagaata gctaagacac tctcatttaa aataggagat gatgcaattt taattgtgac
      901 agctggtgat gctaagattg ataataagaa gtataaggct gaatttaatt gtaaggctaa
      961 aatgttgaca ccagaagaag tcttggagtt tactggacat gctattggtg gagtctgtcc
     1021 atttggttta aaaaattcta taaaagtcta tttagatgat tcaatgaaaa gatttgatac
     1081 agtatttcct gcatgtggca gtagtaactc tgcaatagaa cttacatgtg aagaaatgga
     1141 gaagttttct aagtgtgaaa aatgggtaga tgtttgtaag aattggtaaa aaaatatagt
     1201 aaactatagt aggaattagt aaaaaactac ctctgtatac aatctataca gaggtagttt
     1261 ttttgtgtaa atatttataa atctaagagg aaaatctaat ttaaactaat aatccaaaat
     1321 aaagttaaaa aattctgatt ttttttcgaa acaataaaaa aatgacaaaa aagtggaaaa
     1381 aatgtaataa atattgttaa aaaaataatt agaaattatt gaaaaattat cctttgagtg
     1441 atactattat agatgtaaga tatttaaaaa taaaaaatag tcagataaaa atacgattat
     1501 ttagctctat taaaataatt tttatacgat aaaaatcttc tgtacatggc taaaaaacag
     1561 atgtacagac aatatttaat gtttaatata ccctaatata aatatcgtaa acaaattatt
     1621 ttatctgtaa ccagtgtgaa agaaatctta ttctaacttg aaatttcaca aaattccaaa
     1681 aagcgagatt ttttttaagt atagttgatt ttaaattaac aatttatgat taaattaatg
     1741 tcataattgg ttagtataat cacatataaa tatagagcgt agtttgatta aggttataaa
     1801 tttctcgacg tatattcaaa taccttatta tagatgttgc cgagatttta attaataaat
     1861 gaaaataaaa tatttataaa atactgagga ggctattatg gatttaaatt ctaaaaaata
     1921 tcagatgctt aaagagctat atgtaagctt cgctgaaaat gaagttaaac ctttagcaac
     1981 agaacttgat gaagaagaaa gatttcctta tgaaacagtg gaaaaaatgg caaaagcagg
     2041 aatgatgggt ataccatatc caaaagaata tggtggagaa ggtggagaca ctgtaggata
     2101 tataatggca gttgaagaat tgtctagagt ttgtggtact acaggagtta tattatcagc
     2161 tcatacatct cttggctcat ggcctatata tcaatatggt aatgaagaac aaaaacaaaa
     2221 attcttaaga ccactagcaa gtggagaaaa attaggagca tttggtctta ctgagcctaa
     2281 tgctggtaca gatgcgtctg gccaacaaac aactgctgtt ttagacgggg atgaatacat
     2341 acttaatggc tcaaaaatat ttataacaaa tgcaatagct ggtgacatat atgtagtaat
     2401 ggcaatgact gataaatcta aggggaacaa aggaatatca gcatttatag ttgaaaaagg
     2461 aactcctggg tttagctttg gagttaaaga aaagaaaatg ggtataagag gttcagctac
     2521 gagtgaatta atatttgagg attgcagaat acctaaagaa aatttacttg gaaaagaagg
     2581 tcaaggattt aagatagcaa tgtctactct tgatggtggt agaattggta tagctgcaca
     2641 agctttaggt ttagcacaag gtgctcttga tgaaactgtt aaatatgtaa aagaaagagt
     2701 acaatttggt agaccattat caaaattcca aaatacacaa ttccaattag ctgatatgga
     2761 agttaaggta caagcggcta gacaccttgt atatcaagca gctataaata aagacttagg
     2821 aaaaccttat ggagtagaag cagcaatggc aaaattattt gcagctgaaa cagctatgga
     2881 agttactaca aaagctgtac aacttcatgg aggatatgga tacactcgtg actatccagt
     2941 agaaagaatg atgagagatg ctaagataac tgaaatatat gaaggaacta gtgaagttca
     3001 aagaatggtt atttcaggaa aattattaaa atagataaat attaaaatat acacaatagg
     3061 tcgatattaa aatagatatg gaggaaggat ttatgaatat agtcgtttgt ataaaacaag
     3121 ttccagatac aacagaagtt aaactagatc ctaatacagg tactttaatt agagatggag
     3181 taccaagtat aataaaccct gatgataaag caggtttaga agaagctata aaattaaaag
     3241 aagaaatggg tgctcatgta actgttataa caatgggacc tcctcaagca gatatggctt
     3301 taaaagaagc tttagcaatg ggtgcagata gaggtatatt attaacagat agagcatttg
     3361 caggtgctga tacttgggca acttcatcag cattagcagg agcattaaaa aatatagatt
     3421 ttgatattat aatagctgga agacaggcga tagatggaga tactgcacaa gttggacctc
     3481 aaatagctga acatttaaat cttccatcaa taacatatgc tgaagaaata aaaactgaag
     3541 gtgaatatgt actagtaaaa agacaatttg aagattgttg ccatgactta aaagttaaaa
     3601 tgccatgcct tataacaact cttaaagata tgaacacacc aagatacatg aaagttggaa
     3661 gaatatatga tgctttcgaa aatgatgtag tagaaacatg gactgtaaaa gatatagaag
     3721 ttgacccttc taatttaggt cttaaaggtt ctccaactag tgtatttaaa tcatttacaa
     3781 aatcagttaa accagctggt acaatataca atgaagatgc gaaaacatca gctggaatta
     3841 tcatagataa attaaaagag aagtatatca tataatttaa ggggggataa agaatgggta
     3901 acgttttagt agtaatagaa caaagagaaa atgtaattca aactgtttct ttagaattac
     3961 taggaaaggc tacagaaata gcaaaagatt atgatacaaa agtttctgca ttacttttag
     4021 gtagtaaggt agaaggttta atagatacat tagcacacta tggtgcagat gaggtaatag
     4081 tagtagatga tgaagcttta gcagtgtata caactgaacc atatacaaaa gcagcttatg
     4141 aagcaataaa agcagctgac cctatagttg tattatttgg tgcaacttca ataggtagag
     4201 atttagcacc tagagtttct gctagaatac atacaggtct tactgctgac tgtacaggtc
     4261 ttgcagtagc tgaagataca aaattattat taatgacaag acctgccttt ggtggaaata
     4321 taatggcaac aatagtttgt aaagatttca gacctcaaat gtctacagtt agaccagggg
     4381 ttatgaagaa aaatgaacct gatgaaacta aagaagctgt aattaaccgt ttcaaggtag
     4441 aatttaatga tgctgataaa ttagttcaag ttgtacaagt aataaaagaa gctaaaaaac
     4501 aagttaaaat agaagatgct aagatattag tttctgctgg acgtggaatg ggtggaaaag
     4561 aaaacttaga catactttat gaattagctg aaattatagg tggagaagtt tctggttctc
     4621 gtgccactat agatgcaggt tggttagata aagcaagaca agttggtcaa actggtaaaa
     4681 ctgtaagacc agacctttat atagcatgtg gtatatctgg agcaatacaa catatagctg
     4741 gtatggaaga tgctgagttt atagttgcta taaataaaaa tccagaagct ccaatattta
     4801 aatatgctga tgttggtata gttggagatg ttcataaagt gcttccagaa cttatcagtc
     4861 agttaagtgt tgcaaaagaa aaaggtgaag ttttagctaa ctaatttctt ggggagggta
     4921 ttatgagtac aagtgatgtt aaagtttatg agaatgtagc tgttgaagta gatggaaata
     4981 tatgtacagt gaaaatgaat agacctaaag cccttaatgc aataaattca aagactttag
     5041 aagaacttta tgaagtattt gtagatatta ataatgatga aactattgat gttgtaatat
     5101 tgacagggga aggaaaggca tttgtagctg gagcagatat tgcatacatg aaagatttag
     5161 atgctgtagc tgctaaagat tttagtatct taggagcaaa agcttttgga gaaatagaaa
     5221 atagtaaaaa agtagtgata gctgctgtaa acggatttgc tttaggtgga ggatgtgaac
     5281 ttgcaatggc atgtgatata agaattgcat ctgctaaagc taaatttggt cagccagaag
     5341 taactcttgg aataactcca ggatatggag gaactcaaag acttacaaga ttggttggaa
     5401 tggcaaaagc aaaagaatta atctttacag gtcaagttat aaaagctgat gaagctgaaa
     5461 aaatagggct agtaaataga gtcgttgagc cagacatttt aatagaagaa gttgagaaat
     5521 tagctaagat aatagctaaa aatgctcagc ttgcagttag atactctaaa gaagcaatac
     5581 aacttggtgc tcaaactgat ataaatactg gaatagatat agaatctaat ttatttggtc
     5641 tttgtttttc aactaaagac caaaaagaag gaatgtcagc tttcgttgaa aagagagaag
     5701 ctaactttat aaaagggtaa aaatctaatt aaaaatatat ttgctgttaa ctagattaaa
     5761 tagtatttta atctagttaa cagaatataa atactaaaat aacaggaggt tataactatg
     5821 aaattagctg taataggtag tggaactatg ggaagtggta ttgtacaaac ttttgcaagt
     5881 tgtggacatg atgtatgttt aaagagtaga actcaaggtg ctatagataa atgtttagct
     5941 ttattagata aaaatttaac taagttagtt actaagggaa aaatggatga agctacaaaa
     6001 gcagaaatat taagtcatgt tagttcaact actaactatg aagatttaaa agatatggat
     6061 ttaataatag aagcatctgt agaagacatg aatataaaga aagatgtttt caagttacta
     6121 gatgaattat gtaaagaaga tactatcttg gcaacaaata cttcatcatt atctataaca
     6181 gaaatagctt cttctactaa gagaccagat aaagttatag gaatgcattt ctttaatcca
     6241 gttcctatga tgaaattagt tgaagttata agtggtcagt taacatcaaa agttactttt
     6301 gatacagtat ttgaattatc taagagtatc aataaagtac cagtagatgt atctgaatct
     6361 cctggatttg tagtaaatag aatacttata cctatgataa atgaagctgt tggtatatat
     6421 gcagatggtg ttgcaagtaa agaagaaata gatgaagcta tgaaattagg agcaaaccat
     6481 ccaatgggac cactagcatt aggtgattta atcggattag atgttgtttt agccataatg
     6541 aacgttttat atactgagtt tggagatact aaatatagac ctcatccact tttagctaaa
     6601 atggttagag ctaatcaatt aggaagaaaa actaagatag gattctatga ttataataaa
     6661 taagaatttg ggaattaaaa atttaaataa aattgtttaa aaaacaattt cggatatatg
     6721 aaaaatctaa tttaatgggg gtaatgaata tgagagaagt agtaattgcc agtgcagcta
     6781 gaacagcagt aggaagtttt ggaggagcat ttaaatcagt ttcagcggta gagttagggg
     6841 taacagcagc taaagaagct ataaaaagag ctaacataac tccagatatg atagatgaat
     6901 ctcttttagg gggagtactt acagcaggtc ttggacaaaa tatagcaaga caaatagcat
     6961 taggagcagg aataccagta gaaaaaccag ctatgactat aaatatagtt tgtggttctg
     7021 gattaagatc tgtttcaatg gcatctcaac ttatagcatt aggtgatgct gatataatgt
     7081 tagttggtgg agctgaaaac atgagtatgt ctccttattt agtaccaagt gcgagatatg
     7141 gtgcaagaat gggtgatgct gcttttgttg attcaatgat aaaagatgga ttatcagaca
     7201 tatttaataa ctatcacatg ggtattactg ctgaaaacat agcagagcaa tggaatataa
     7261 ctagagaaga acaagatgaa ttagctcttg caagtcaaaa taaagctgaa aaagctcaag
     7321 ctgaaggaaa atttgatgaa gaaatagttc ctgttgttat aaaaggaaga aaaggtgaca
     7381 ctgtagtaga taaagatgaa tatattaagc ctggcactac aatggagaaa cttgctaagt
     7441 taagacctgc atttaaaaaa gatggaacag ttactgctgg taatgcatca ggaataaatg
     7501 atggtgctgc tatgctagta gtaatggcta aagaaaaagc tgaagaacta ggaatagagc
     7561 ctcttgcaac tatagtttct tatggaacag ctggtgttga ccctaaaata atgggatatg
     7621 gaccagttcc agcaactaaa aaagctttag aagctgctaa tatgactatt gaagatatag
     7681 atttagttga agctaatgag gcatttgctg cccaatctgt agctgtaata agagacttaa
     7741 atatagatat gaacaaagtt aatgttaatg gtggagcaat agctatagga catccaatag
     7801 gatgctcagg agcaagaata cttactacac ttttatatga aatgaagaga agagatgcta
     7861 aaactggtct tgctacactt tgtataggtg gaggaatggg aactacttta atagttaaga
     7921 gatagtttta gattataata tttacaaatt aaatatttaa ttatgaattt aacctgtaag
     7981 aataactatt tgttattctt acaggttttt atagttaaaa ataattatta tctattaaac
     8041 aaaaatagtg taaaatatat aatatcatga ttttgaagat ataaaaagta ggaagatata
     8101 aaagaaagta ggtaaaagtt tgtttaaaaa agagaatcaa agatataatc ttatatcgta
     8161 tacaaatgaa gaagaaatga ctttaaaaga agttttattg gacaagctaa atttctcagt
     8221 taggtcatta tcaaaaatga agagggaaaa gagtgtttta gtaaatggag tatataaaaa
     8281 accgagttta aaggtgtata gtggtgattt aatagaagta aaaatagacg aggaaaaagc
     8341 aaattttgaa cctcaagatt taaatttaca gataatttat gatgattttg atataattat
     8401 ggtgaataaa cctccgttta tggtagttca cccaacaaaa agccactatg ataaaacaat
     8461 agcaaatggt attagttact atatagataa tcaaaaagaa aatgtaaaaa taagatttgt
     8521 aaataggtta gatatgaata cttcagggtt agttatagta gcaaaaaatg cttatgcaca
     8581 tcatactttg tctacagcaa tgagtgaaaa taaagttgaa aaaaagtata taacagtagt
     8641 tgatggaatt ataaaagaaa atgagggaac tatagatgag ccaatttata gacctacaga
     8701 agattcaata aagaggataa tagatgaaag aggacagtca tctgttactc actataaagt
     8761 aatagaaaga ctagaaaatg caactgtatt agaagtaagc ttagaaactg gtagaacaca
     8821 tcaaataaga gtacatatgg ctcatatagg tcatggaata ataggtgatg agttgtatgg
     8881 ttatgtagat gaagagctaa taaataggca agcacttcat gcatataagt tagagtttga
     8941 acaaccaaga acaaaagaaa aattgaaatt taaagcagat ataccagagg atatgaaaga
     9001 gttaatatcg aaattaagat aatggggtga tatatatgat aatacataaa tttataatac
     9061 atgttttgga caaaaatagt gatgttccaa tattaaatga ctttgaaggc aaagttaatc
     9121 aagaagtaga tggttttttc cagaaagcta taaaaagaat tgcaaaagat gaggatttaa
     9181 gaaaaggtgt ttttaaagat tataatgaca atttaattaa aaactgttgt gaacaaataa
     9241 tatatgatga aagtactttt ttgaaaaatt ctaaagaaat tgcatcttac ttgtttgacg
     9301 ttatgaaaat taatgctgca ctagaatctt gtgatttggc aatttgttta tacactatta
     9361 aagatgaaaa gagtgtagca atactgaaat tagattataa aaagttgtac actcattcaa
     9421 ttgaatttgt ggatgataaa tttaatattc aaatggtttc aaatgaaatt ggtatcccag
     9481 agactttaag acaaaagcaa ggagctttga ttagtctaag tggtataaat gatgagtttc
     9541 atctaaggct tttagataag gatgctgaaa aagaagggtc agaatctaag tttgtaacag
     9601 aatttttaaa tgctaaaaaa atagatgatg ataagtacaa gaccaaggta tttaaaaata
     9661 cagctgaaaa ttggataacc aatgctctta gtaatgatat aaagcaagct gaagatgtaa
     9721 gaagtatatt aaattataca ctgaaggaaa aacatgaagt tgatataaat gactttgttg
     9781 ataattcaat taaagatgac gagttaaagg atagctttaa agagcatatg gaagaaaaag
     9841 gtcttgatga aagttttagt atagataaaa aatgggttga gaaaaagctt aaaaaaagaa
     9901 gtataaaaac ggataatggt tttgacataa aagggaatct aactgatttt gaagacccaa
     9961 tgaagtatac tgtaaaacaa aatcaagatg gaactataga tataataata aagaatgtta
    10021 cgttttatga agaaaagtaa tttatatact tagtatataa attaaatata agagcgtgta
    10081 ttttatatat tgtaaataaa tgtaatctag tctgtgcgaa ctctaatata atttattatg
    10141 tagaagttta cacagactat ttatatttgt agacaaaacg atactatttg ttttcttcga
    10201 tatatttaca tatatcacca atacatttaa agttctcagc ttcctcgtct ggtatttcta
    10261 ttccaaattc atcttcaagg gccattataa tttcaacagc atctaatgaa tcagcttcta
    10321 agtcgtccat taaagatgct tccatagtta tctcatctaa attgtctacc cctaattgtt
    10381 ctgctattat ttcttttatt ttctcaaaca ttttaagcct cctaaagcag ttttaattaa
    10441 ttttaatgtt agtttaatat aatatcaaga atttttcaat atggaatcta taaaaaataa
    10501 taaaatttta taaatgtagt aataaagtat tatttgatga ataaacttta ttgataacaa
    10561 atataactta ggaggaaatg ctatgaaata tgtaggacta ttactaactt cagtaggaat
    10621 gtttttactt atagcagtaa atttttatta taattcaata actttagata tgcaacgtat
    10681 agaggattat gtaatggaga ccaatttaat ccttgaagat gtagctgaaa aggagagtta
    10741 tgtatctaat gaaaaagaag actacatatc aaggttaatg catgtaaaga aaggaataga
    10801 aaattcaaaa acatccttct tgatagaaag atataaggag tacaaaataa aatcaataga
    10861 aagtttaatc tatactatat cagaggagaa aaaagattat ctggatgaag ttgatagata
    10921 caataaactt ggtgaaaaag aaataaataa actaatcaat aaaaactttt tagaagtaac
    10981 ttatctgtct attactacat atatttaatt aaagagacaa aacttagtct cttaatgatt
    11041 caaggaggat tagttatgac aaaattagaa gacaatatgg aacgtttctt tggaggatgc
    11101 gaaggatttt ttgggaataa aatgttgata ataatagtta tagtattctt actattatgc
    11161 acagacattt tagaagactt attatgcgat gacaatatat ggatatgggt tatattaata
    11221 gtattgttat tatttaactt cgatgatggt tgttgttgct aaaaaatatt gattattaaa
    11281 taggtaaaac cagtaactta tacaagttat ggttttacct tttttagttc aaattaaaaa
    11341 atttaaaaaa atagtaaaaa tacattagca aaatggaaac caaatgcata gtatatgtta
    11401 ggagcgaaaa gctcaacaaa atatcaacag gaggtaacgt ataatgttag atagattctt
    11461 tggagaagaa ggatgttttg gtggaggagg atggtggata atagtactat tcttcctatt
    11521 cttagctttt ggagatagct gggcagaatt tgacataatg gcatggatac cattcctaat
    11581 agttttatta atactttgct gctgtggtgg atgcttagaa taagaacaca gttattaaaa
    11641 aaaggagggc tttgttatgt tagatagatt ttttggagaa gaaggatgct ttggaggagg
    11701 aggatggtgg ataatagtac tattcttcct attcttagct tttgaagatt gctggagaga
    11761 cattgacata atgtcatgga tacctttctt aatattattg ctaataactt gttcatgtgg
    11821 tggattcttt gatggtgaag taggttgcgg ctgctaatac ttaaatcatt agaataaaaa
    11881 aatgctggta ttttatacca gcattttttt attttatttt ttaatatgat ttaaattaag
    11941 aatgaatatt attaaatttg ttgaatatag ggtataatat aaaatataat gtatttaaat
    12001 gtacttgctt ttatggtaaa ataaaaactt atataaatgt agttaaaata ggaaccttca
    12061 aaaatttggt tcagttttag ataaatttag taaaataagg aggatgaaga tgaaaggcaa
    12121 tataacgata aaagatgttg ctaaacaagc aggagtgtca atatctactg tatctagagt
    12181 tataaatgat tcaaaacctg taactgatga agtcaaacaa aaagttttag aggttataaa
    12241 agagactgga tatataccaa atccacttgc tagaagctta gtaacaaaga agagtcaatt
    12301 aataggggta atagttccag aagtttcaga ttcttttgtt aatgaggtgt taaatgggat
    12361 agaagaggtt gctaaaatgt atgactatga tattctttta gcgaatacat actctgataa
    12421 ggaacaagaa cttaagagta taaatctatt gagagcaaaa caagtggaag gtatagttat
    12481 gatttcatgg atagttgaac aagaacatat caactatata caaaattgtg gaataccagc
    12541 gacatatata agtaaaactg ctagaaatta tgatatatat acagtaagta ctagcaacga
    12601 agaagctact tttgatatga cagagcatct tataaagaaa ggtcatgaaa agatagcttt
    12661 tataatgacg agtaaagatg atactgtttt agaaatggaa agacttgctg gttatgagaa
    12721 agcactttca aataacaata tagaattaga caagagtttg attaagtatg gtggaactga
    12781 ttatgagagt ggatacaata gtatgaaaga actattagat gatggaataa tacctcatgc
    12841 ggcttttgta acaggtgatg aggctgccat aggtgctata aatgctatat gtgatgctgg
    12901 atataaggtt ccagaagaca tatctgttgc aggatttaat gatgttaaga tagctagaat
    12961 gtatagacct aaacttacta cagtatatca acctctatac gatatgggag cagtagcaat
    13021 aagaatggtt ataaaattaa taaataagga attaattgaa aataagaaaa tagaattacc
    13081 ttatagaatt gttgatagag aaagtgttac agaaagaaaa aaataaaact attaaaatca
    13141 atcaggtagt gcctaaggca ctacctgatt ttttatgtca ataatatcaa atttaataat
    13201 agattaacta aaaataatta tctaattact taaatagttt atttttatct attcatgagg
    13261 gttagacttt ttagttttct taaataaact tttagatttt ttttgttgac tacctggtgt
    13321 attttttggt gcaggtgaat ttgacttacc tttagtaggt ttagccttct tagttttagc
    13381 tggtttagta tctctttggt tttctgcatc tgagattttt tccattatat tatccagttt
    13441 tttgcgttga ttttcatcta aaaatgcttt gaattttgat actacttcat ctctgccatc
    13501 tagattctta cctattttta taagttcctc cataagttca ttttctgatt ttcctttgta
    13561 actatcagcc atcttttcta ttttatcctt gtcaatattt ttttgtttag ccattttttc
    13621 taatgactct gtatctaatt ttttcataaa aaaacccctt tctataaata tatattgaat
    13681 tatattcata ctatttatat atagaaataa tattgaagtg gtgaattgtg agtgatatat
    13741 aatataaata aaaattctta tagataatat atatgcactg agctttaaaa atggaacatt
    13801 aaaatatgaa aaacatactt taatataggg caattatata atataagggg gaattgtctt
    13861 gaataactta ctatttttga ttggagtgat tgcattgagt aatggtaata tctctatatt
    13921 tgatgataaa aataaatcaa aaggaaataa tagagttaga aaaaacaaaa aaaatgcaaa
    13981 tgataaactt tcacagtcta cacatggcaa tttaaggttt gatgcaaatg atataaaaag
    14041 aggtttaagg ctcatggatt taagtgaaga agatttagaa atgggaatgg aaataataac
    14101 aagaactaaa aaatacatgt caagagatga aagaaaaata ctaataaaaa tagaaagtgt
    14161 tttagattta gttagaggaa taaaaaaatt aaataatata gatatggtag atacagaaga
    14221 tgaaacggat tttttcagaa aaatggatag tgatgataaa aagaatatga tgataaagga
    14281 aattatagat gtattcccag aaaaaagaaa aaattcagtt gaaaaagcta taggaatgaa
    14341 aaagaagata gatttatttg cagaattatt tttaccagat gactttggtg aaggtggatt
    14401 tagtttgagc tctttggcaa atataaacaa tcttggtagt atgaataatc tcaaattgtt
    14461 aggaagtctt ttaagaggtg atgatagtaa taatgatgat gaagacaata atgatgagtt
    14521 aaattatgaa gacgaagaat tagattgtga agacaaagaa ttggatgatg atgagttaac
    14581 agatgaaggt gaaatggagg ataatgaaga tgaattagta gaagataaga acacaggcta
    14641 tgaaaatata gaaaatgatg aagatatagt gtacgatgag gaactataca acattaatga
    14701 gcaagaatcc gaatacaatt ataatgaaaa aaatactaaa actagagcta agaaaaaacg
    14761 ataattaact aaatacataa agaaaaaagg aagaaaaagt aatgatagaa taagattatc
    14821 tcaattacta tataaaacca ctttaaaaaa ttcaaaattt attatataat aataatatga
    14881 aatcctgaaa catacgataa ggctttttta aattcaacct acaaatgaaa tacgggagac
    14941 cgtgtctggt aaattataac atgcctagtt agtttggacg ttaaaggtta ctggcaagtc
    15001 acccacctgg gagtgcctag ggtgtgaatt tacttagcca ccggtgtttc gggacaaaaa
    15061 aagtgctact atttcaaata gtagtacttt ttttattaat atgcattttt ctactaaata
    15121 cgcctaatta ttgataaatt caaaaagcaa gtaacaatta agattataaa agcatagtat
    15181 taattaggct tcaaaaagcc aaagacttaa taagaatcgc ttacagggag ggcaatgaaa
    15241 aatgcaagat tataaaaaaa ataaaagaag aatgatgaat cagccaatgt ctacaatgaa
    15301 tgaagaagaa gtgtatacag atgaaataaa ttcagaagac atgagaggtt ttaaaaaatc
    15361 acaccatcat aatggatgta atactgataa taagtgtgag tgccatgatg attgcaatcc
    15421 atgcaaccca tgtaatccat gtaaacctaa cccatgcaat ccatgcaaac ctaatccatg
    15481 tgatgacaat tgtggatgcc atgacaattg taaatgtgat tgtgaaccat gtgaaatgga
    15541 ttcagatgaa tgttttgaaa acaaatgtgg accagaatgc tgtaatccta tatctccaag
    15601 aaacttctct gtatcaaatg cagtgccatt tgcaatagag gctaatagaa tatttgatac
    15661 tatgcaattc caaacattta cagatgcaac aggaccaaat ggagagccat taacttttga
    15721 aacagaagta gtagaagtat ttggttcagt tccaagtgca ggtcaagcaa gtgtaactat
    15781 agaaaaaata tgcttaagta atgatggaat cgttatagac acaggaatga caactttaga
    15841 agatttcgat ttagacccat taggagatat agtaggaaga aactgtgaaa caacttttga
    15901 atttgcagtt tgtggagaaa gaaactctga gtgctgtaga caaggaaaag gcaaatcagt
    15961 agcttataaa caaagaggat taactgtagc agttcgtaat ttagtactag agctaagagg
    16021 tagatgtgga tgtacagagt tcgttgcatt agctttccca gcagttagag caggaggtgg
    16081 atgtaagaga agagttgatt atgtagaatt tacttttaac acactttcag caccaatatg
    16141 cttgccagct gacggaagag ctgttacttt aagacaagaa tatcaaacta acttaactgt
    16201 agattgtata ggaaaatcta tattaaaatt agaatgcaac gaatgttgtg aacctttcta
    16261 tgaattaatt ataccaaatg atatagattt agtactttgc ttacaagaaa cagttagcac
    16321 attaataagt gaacaaatag tagttttagc atcaccaaat ccaatccaac caagacttgt
    16381 tgatactttc tctaaagtat gtgatttttc gcaatgtgga cctaatcatg gaagtggaaa
    16441 gccaagttgc cacagataga taggtagata tatagacagt ttaaattata aataaaaatc
    16501 atctaaggaa ggctaccaaa gtgtataatt tacattggta gccttcacgt ttttataact
    16561 agcgtataga ttttagtgat ttaaaaagca ctatagtatg taatagacaa gtaaagattg
    16621 atactatcgt cattgtgtat atgtatgcat aaatattaag tcctggaatt ggaagaaata
    16681 catataaagc tattagttga attaacatgc cagtaatagt atttatactt gaagctaatt
    16741 cttttcttat tgaatgtaaa atgccagata gagtttgatt tagggacata aatagtggaa
    16801 ctaaaaacat agcttttaga tattctcctg caagtgtatt tttaaatact aatatacata
    16861 aatcttttcc aaagaaataa aaaaatattg atgatagtat gcctacaaat aaagtaagaa
    16921 gtatggagta tttgattttt ttaattacac tcttacgctt tcttagagcc atttcctgtg
    16981 aaatagttgg tattaaattt acaataagcg ctgaaccaac agtaaaaggt aaaaatataa
    17041 aaggcattac catcccactg acaataccat acatacttaa agattgttga taagtcattc
    17101 ctgataatgc taatcttgat ggtatcatca tagaacttat agattgcaaa agtatgtttg
    17161 acattctatt acaagttatg ggaatagaca tttttaatgt ttccatagaa gaattataaa
    17221 aatcttttat gcttataata tacctattgt caaatgatga atctcgccat aagcaaataa
    17281 gcataaatat aatattactc aactcaccaa tcgatatacc taatagagct atataacaat
    17341 tcatagattt attatttatg tacataacaa gtaaaaaaac aaataatatt ttaccaattt
    17401 gttcaatgat ttgtccaata gcagggattt ttacattctt tatgccatag taatatcctc
    17461 ttagtacgtt tgaaagagtt attataacta tagcaggaca aacagctagt ataaatagat
    17521 ttaatttagc atcttttaaa aatttaagtg ataaaaaaga actattgaaa gatgcaacta
    17581 tagatataat tagtgctacg aaaaatgata tatataaagt tgatataaat aaagcattag
    17641 tattgtgtct atcgtttaaa gcttttctct ttgcaactag acaactaagt gcagtaggta
    17701 tacctgttgt tgtaacagct aagcaaatca tcaaaaagtt aaaaatcata tgatagatac
    17761 ctaaacctgt ttcacctagt gctcttgata agaatatttt atataaaaaa ccaaatattc
    17821 ttacaataaa attggatata aataaaataa ctgttgatag tactaaataa ggtactttca
    17881 aaaaaatcac ctccttatct tttaaatata ttcacaagga ggtgtaaaaa tttataattt
    17941 tataaattct ggtttcaatt tattaaattt agatacatat ttaaatccca tattttttag
    18001 taatgaatat atgtagtcaa actcacatgc aacttgactt atatgatgtg aatctgaacc
    18061 tgtggttatg atttcccctc ccaagtcttt atacatttga agtatatact tagatggggt
    18121 taggtctgga agattatatc tataacaaga ggtatttatc tcgatacctt tgccatcata
    18181 aatagcttgt ttaagagttt cttcaattat atcagaaaat agtctatcat ctaaaacagt
    18241 gtcataaggt gcataccttt taattaaatc taaatgacct aagacagagt aatctttata
    18301 attcttaaca atattatata aataaagata gtaattttca taaacttcat gttgagtttt
    18361 atctttaaaa taattaccaa ggtataagtc cattgtatct atagcatgga tagaacatat
    18421 tataaagtcc aatggatatt gatgtgcttc ctttttgtat gtatccatta attgagtttg
    18481 aactccaaat tcaattcctt tttttattga tattttatct ttatatttgc tttgaagagt
    18541 ctctaaactc ttaaaataat cttcatagac aattgaaaaa gaatcatcag catatacatc
    18601 atagtcaaca tggtctgtga aacaaatttc ctccaaaccg agttcaatag atttttttac
    18661 catttgttcc attgtagatt ttccgtctgt agagaaactt gagtgcatgt gatagtcata
    18721 aaacataaat atttcccctt tttataaaaa ttttggtatt atatatacat actaaataat
    18781 atcaaatata cataaaagca tcaataaaat ataaatacaa tgagcggtga taaatttgga
    18841 aaaatacaga atagatttaa atgagaaaaa atcaactaaa tatatacaga tatttaatca
    18901 tataaaagaa ttgataataa ataaagagtt agtagaacat gaaaaattgc ctccaataag
    18961 aaaactatca aattttttac aagtaaataa cactactata gttaaggtat acgagctttt
    19021 agaaaatgaa ggatatgtat ataaaattat agggagtggt acttttgtat caagtttaaa
    19081 ttcttataaa agcggtatag ataacaggaa aaataagatt attgtgcaaa gagaagattt
    19141 aattcatttt gacaatggga atccatctaa tgatatgttt cctatcgatt catttaaaaa
    19201 tgctgtaaat atggcattat ctaaagatgg tagttcaata tttgaatatg atgaaggtct
    19261 tggctctgaa gaattaagag aaaaaatggt agagtatcta agtgatggaa atataaaaac
    19321 tactaaggaa aatattcaaa taatatcagg tgctcaacaa ggtatagata tagtttgtaa
    19381 aggacttata agttattcag atgttgtttt tatggaagaa ccttcttaca atggagcaat
    19441 tgaagtgttt aaaagtagag gtgcaaaaat aatatctata cctatgttag atgatggaat
    19501 tgatataggt attttaaaat taaagttgga aaaaataaaa ccaagactta tatacattat
    19561 gcctaatttt caaaatccaa ctggaatatc ttactcaaca tataaaaaaa agaaattgat
    19621 agaattggca gaagaacatg atttttatat aatagaagat gattttataa gtgattttgt
    19681 ttttgaatca aaggacaata gaacaattag aagttatgat gataaaaata gagtagtata
    19741 tataaaaagc ttttcaaaaa tacttatgcc aggtcttagg ataggaattg tagaaatgcc
    19801 aaatgagttg ttaaaaaaag ttttatgggc taaatattca tcagatatat caacaccagg
    19861 tttaattcaa aaatcaatgt attactatat gaataatttt gattggaaga attatttgag
    19921 tcatatagag aagatatata ctgataagta taaattggca aaaaacttga ttaatgacaa
    19981 attgagtggt aaattaaaag ttagaaaatc ttgtggagga ataaactttt ttttagaatt
    20041 accaagagga tatacatctc aagcatttac aaattttatg ttaaacaaag gagtgtcaat
    20101 gcttccaggg acttattttt ttgacaatct agtggatgat agattcttta ggataaatat
    20161 agctcgtcca agcatggaag aactagaaaa gggaatatct ataataagtg ataatataga
    20221 tgagtttttt tgtgaatata aaaacaagtt agatataaaa agcaataaat tattttatta
    20281 aataaataga ggttttttag gaggaaaaaa tgtttgacaa gaagaaatta gataggatta
    20341 atgaattagc aaagaaaaat aaagagggaa tcctttctgc tgatgaaatc aaagaaagag
    20401 aaattttaag gaaagaatat ttagaaaact ttagagccca ctttagatca agactagata
    20461 gtgtcaaagt agtttcacca gaagaatatg agcaatacat gaaaaacaat aaaaattaga
    20521 acttagtagg aggaaaatat gaacttaaag tacgaattta agaatgcttg ggattttgaa
    20581 agaaaagaaa atactattga acaaattatg caatattctc gcaattatat ggagttttta
    20641 agtaagtcaa agactgaaag actttcagta aaagaaatta taaaattggc taaagaaaat
    20701 aactatattt caatagatga agctatggaa aaaggaagca taagttgtgg tgataaaatt
    20761 tatgttataa ataaagagaa ggcagtagca ctttttgtaa taggtaaaaa ttatatagaa
    20821 aaaggtatga agattatagg aagccatata gattcgccaa gattagattt aaaaccaaat
    20881 ccactgtatc aagagagtaa tctaggtttt tttaagactc attattatgg tggaataaaa
    20941 aaataccaat ggacggctat tccacttgct ttacatggta tagttatatt aaatgatgga
    21001 acaaaagtgg acatatctat tggggaagaa gatagtgacc cagtattttg tgtaactgat
    21061 ttattgattc atttggctgg agaccaaatg caaaagaaac tatcagaagg aattagtgga
    21121 gaagctttaa atatattaat tgggaatatt cctttagaag atgaagaaaa agaacctata
    21181 acagcaaata tattaaagat tttaaatgag aaatacaata tagttgaaga agatttatta
    21241 agtgctgaaa ttgaagtagt tccagctggt aaggctagag atttagggct tgataggtct
    21301 atggtgttgg gatatgggca tgatgatagg gtatgttcat atgctgctgt aaaagctata
    21361 ctagaaactg aacaaccaga atttacttca gttgcattat gtgtagataa agaagaaatt
    21421 ggttctaaag ggaatacagg tatgcactca aagttttttg aaaatactgt ggctgaactg
    21481 attgctttag aaggagatta ttgtgatatt aaagtaagaa gagctttagc aaatagtaag
    21541 gtattatcag ctgatgtttc tgctggatat gacccaaatt ttggagaagc atatgaaaag
    21601 agaaattcgg catatatggg aaatggagta gttttaacta agtatacagg ttcaagagga
    21661 aagagtggat gtaatgatgc taatgctgaa tttatgtcag aagtaaagag aatttttaat
    21721 aaaggaaatg tagtatggca aactgctgaa cttggaaaag tagaccaagg aggaggcgga
    21781 actatagccc atatattggc aaatcaaggt gcagaggtta tagactgtgg tgttggagtt
    21841 ttaaatatgc atgcaccaca tgaaattgtc tctaaagtag acatatatga aatgtataag
    21901 ggatataagg cattttttaa tataaattta taagtaaaaa tataaatgta ataagtgaat
    21961 aaaggctgtt gtataaacaa tttaatctgt tgtacaacag tcttattttt atgtaaaaat
    22021 agcaatttgt cacactagag aaaataaaag aaaatactgt caaattaaaa attatttaaa
    22081 aaaaaattga caacgatttc atcttgttat aatatgtaat aaagaacttg ttatataatg
    22141 ttataaaaat gttacatagg gaggtgagca aaatggatga aaagagacaa aaaaaagaaa
    22201 tggatttaga agtaaattcc tttgttcctt tatatcaaca actatatgac aacataaaaa
    22261 aacaaatagc atctggcata tataaaccag gagataaact tccatctgaa ggagacttat
    22321 gtaaagaatt taatataagt cgtataactg tgagaaatgc acttaatgaa cttgtaaaag
    22381 aagatatatt atgtaaaaaa cgtggtaaag ggacttatgt aacaatacca gagagaatag
    22441 aagcaacatg tgcaggaaat agttttacta attcttgtca tcgtattaat gcaaaaccaa
    22501 gtaccaaaat aatctctgtt ttaataaaaa aagcagataa gcaagtagct gaagctttaa
    22561 gtattgaact agaagagaag gtaatatgta ttaaaagact tagattaata gatgaagtac
    22621 ctgttatttt tgaagttgac tattttagaa tagattacat gtttttatta aaagaagaat
    22681 tagaagataa atctttaatg gaggttatat ctaataatat aagcacatta cctaaacgag
    22741 tagaaaatat atttgaagta aaacattcaa acaaagaata ttctgaccat cttaaatgtg
    22801 caagtaatat gccactattg aaagtaagac aatcggtgta cacagaaaat aatgatgtct
    22861 tatattacaa tgagcaattt ataagaagtg ataagtacaa atatgcagta tcagcagaaa
    22921 tataatctat aatttagtag gaggatgagt atgaaaaaga taatattagc atctcatgga
    22981 gacttttcaa aagggctttt aaatgcagta aaaatgatag taggagattt agcagattgt
    23041 gtcagttcgt atggtttgta ccctggacaa agtgcttcag attttgcttt agaattagaa
    23101 aaaacaatat cagaagataa agaatgtgaa tatataattt tatctgactt atatggagca
    23161 agtgtatgta cagcaatgtt aagattaact aatctacata atgttaggtt attttctgga
    23221 atgaatttga atatggtatt agaacttctt acaaggtttt ctgatgaatt aacagttgaa
    23281 gatatagacc aattagtgga agaatctaga agaggaatac aaagcgtgac attacaaatt
    23341 aaagaagaag aagaggtatt ttagtttatg aaaaaaaagg gaatattatc agtatttaaa
    23401 agtaaaaaac caattatagc aatgatacac ttaaaaggag atactccaga agatatcttt
    23461 gaaagagcta aaaaagagat tacaatcttt gaagaaaatg gtgtagatgg aataatgtta
    23521 gaaaattact atggtaatta ttatgattta gaaagaatac ttgaatatgt atcaaaagct
    23581 aatttatcaa taccatatgg agtaaactgt ttaaatgttg atactatggg atttgaactg
    23641 gcaacaaaat ataatgctag ttatatacag gttgattctg tagtaggtca tgttaaacct
    23701 agagatgaag ctactttaga agagtttttt aaattacaac gttcaaaatg cccagcgtat
    23761 ttaattggag gcgtacgttt taaatatcag ccagtattat cagaaaatga tgttgaagaa
    23821 gatttaaaaa taggaatgac aagatgtgat gctatagcag ttacagaaaa tgctacaggt
    23881 caagaaacat caatggaaaa aatagaactg tttcgtaaaa atttaggaga cttcccattg
    23941 gttatagctg ctggagttac attggaaaat gcaaaaaaac agcttaaatt aggagatatg
    24001 gcaataatag gaagttactt taaagataat tacaaagatt ttggggatgt tagtgtagaa
    24061 cacgtaaaaa cattcatgga tgaaatcaaa aaaattaggg aggaattata atgattaaat
    24121 tagtaagagt agaccacaga cttatacatg gacaagtagc atttacatgg acaaagtttt
    24181 taagtacaga ctgtatattg atagcaagtg atgacttatt aaaagatgaa ttgagaatgg
    24241 caggacttaa aatggctaaa ccatctaatg ttaagttagt aatgaaaagc atagcagatt
    24301 ctataaaagc acttaactca ggtgttactg ataaatacaa cttattgata ctttgtgaat
    24361 ctgtagaaga tgtttataga cttgctaaag aggtaaaagc tattaaatct ataaaccttg
    24421 gtggaacaaa atcagatgat aatcgtgaaa atatatctaa agcagtgcat gtatcaaaag
    24481 atgatataaa gatgattaaa gagttagatt cagaaggcgt aaatgtattt gtacaattag
    24541 ttcctgatga tgatgctaca aatgtaatga aattaatata attttagggg gatgaaaatg
    24601 gaatttacac aagttatttt gataactcta attgcattct ttgcatatat gcatagtttt
    24661 gtaggttcta caatgcataa tagacctata gttgttgcac cacttgttgg tttagcacta
    24721 ggtaatcttc atacaggtat agtaataggt tcaacattag aattagtatt tatgggtgca
    24781 tttcctgttg gggcaagtaa tcctcctgat tttgtgtctg gttctattat tgccacagcg
    24841 tttgtaatat taacgggtca agatgtatca gcagcagttg tacttgcagt tcctatagca
    24901 actctagtat tgcttataga taacttctta atgacagtag tacttacatg gggagctcat
    24961 attgctgacc gttatgcaga agaggggaat attgaaggcg tagaacgtgt gcaattatta
    25021 tttggtatag gaaataaatt aatattagca ataatagtag gtataggatt ttcattaggt
    25081 gttccagtaa tagaaaaaat actttcattt ataccatcat atgtaactca tggaatggat
    25141 gttgcagcag gcgtgattcc agcaataggt tttgccatgt tagctagaat gatgcttaat
    25201 aagaaaacag tagcattttt attattagga tttatattgg ttgcttacct aaacataaca
    25261 gtaacaggag ttgcattgtt tggattagca atagctttaa tatatgttaa ctttgtagga
    25321 gagaaggagg taatagtaga tgacaacgag ttctaagaaa ttagagacaa tttcaccaga
    25381 tagtaaaata acgaggaaag acttttggaa atgttttaga aggtcattga cattagattc
    25441 atcttggaac tacgaacgta tgcaaaatat agcatatgca tacatgatgg caccaataat
    25501 ccgtagatta tataaagatg ataaagaaaa gaaatctaag gctttaaaaa gacatttaga
    25561 atttatgtca gttactcctc atataagtac tcttttggta ggtatatctg gagctatgga
    25621 agaagaaaat gcaaagaata aagaatttga tgctaatagt ataaatgctg ttaaatcaag
    25681 tttaatgggt ccagtttctg ggataggaga ttcatttttc tggggaacat taaagcttat
    25741 agcagctggg gttggtatag ctttagcttc tcagggtaac ataatgggac cgattttatt
    25801 ccttttaata ataaatgttc ctcattttat tatacgctat atatgtctag ataaaggatt
    25861 taagtatgga actcaattct ttaaagatgt gagtggttca agtatagttt caaaagttat
    25921 ggaagcagct tcaatgttag ggcttatggt aattggaggt atgactgcat ctaatgtaat
    25981 gttaaaactt agtgttaatg taggtagtgg agaatgggca gagcctattc aaacttactt
    26041 agaccaaata atgccatgta tgcttccagc tatgatattc ggtattatgt attggttatt
    26101 aggaaaaaag gtaaagacaa ctacgatatt aatttcagtt atgattattt gtatagtatt
    26161 ggctgctatt ggagtagtat aaataaaaaa atcccaagtt catttgaacg agggattttt
    26221 tgactaaatt aaataaaaaa gaaagatggt tcataataat ttataagcat ttatgtgata
    26281 gttataatta gcttattaat tagttataaa ctcaaaatta agcactactt tattaaagtt
    26341 attatattat aatttcgaaa tactcacata atctgtttag aaatttatct tctaatgggg
    26401 aaaaagtttt ctttttagag taaatgaaga aaaactttct agtaaaatca acatccttta
    26461 ttttataaca ttttatttta cctgcattga gataatcgat agctgaaatg tacgatataa
    26521 aggatactcc aagacctaat ctaaccattt ccttaatagc ctcatttgat tctacatgag
    26581 cgattacatt taatttactt actggaaagt tatttttact aagtgtgttt agtattaagt
    26641 ttcttgtacc tgacccttct tttctcataa taaaatttag ataagccaat tctcctatat
    26701 cgatacaatt atttttatta tcaattttaa aatctgaagg agtgataaga acaagttcat
    26761 catctaataa gtctaggtac tctatttgag gattattgat tttagaacca actaatccaa
    26821 agcttattcg ttcatttaaa atttctgata tagcatattg agaatcataa tggcttatgg
    26881 aaaattttac atctggataa ctcatagaaa aactcttcat aaaatcaggt aaaatataag
    26941 tttcaggtat ggaactacat gctatatcta tgataccttc tatttttcct gaatattctt
    27001 ttatatcata aattgcacgt ttgcaattat tcaatatata tatagcatgt tcataaagaa
    27061 tttcaccaga ttttgtgagt gtaataactt tgttgcttct atttataaga acagtttcta
    27121 gctctctctc taaattctgt atgtgtgcgc ttacagtagg ttgagttaaa aaaagttctc
    27181 ttgcagcctt tgaaaaactc tgatgttttg caacagcaac aaatacttca agttgtttaa
    27241 aatccattat ttaatcctcc ttaaattatc taaaactatt ataacatatt tgataaatta
    27301 atagtcaaag gtgattttta aaatagaaaa cctttaaata ttcatggagt ttttgattgt
    27361 ttaggaataa tttggtataa tgttgtaagg tacttacaat aaaaatatcg taaattgaat
    27421 tttcaaaatt caactttaaa gtgtttttta t
//
LOCUS       DAELNY010000037        27148 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.037, whole genome shotgun sequence.
ACCESSION   DAELNY010000037 DAELNY010000000
VERSION     DAELNY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.037"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..756
                     /locus_tag="KPE55_001348"
     CDS             <1..756
                     /locus_tag="KPE55_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG0492440.1"
                     /translation="VAYIFYKIFMFIKDTRAEQVFKGIIFLLLATQLSNTFKLHTVYW
                     ISLKALDYGVIAALIIFQPEFRAGLEHIGRAKFNLFGKNVNTSEETLNRNIEEIVEAL
                     YSLSRQKIGALIIMERETRISDIINTGTIIDAEISRQLLINIFIPNTPLHDGAVVIRD
                     SKVKAAACFLPLTESKDLSKDLGTRHRAGIGVSEVSDCITLIVSEETGGVSIAKAGKL
                     YRDISRERMMNILRSNLKTNTETRSFFKGGIFK"
     gene            753..1934
                     /locus_tag="KPE55_001349"
     CDS             753..1934
                     /locus_tag="KPE55_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492441.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKDDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2238..3140
                     /gene="ptb"
                     /locus_tag="KPE55_001350"
     CDS             2238..3140
                     /gene="ptb"
                     /locus_tag="KPE55_001350"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG0492442.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3162..4241
                     /gene="buk"
                     /locus_tag="KPE55_001351"
     CDS             3162..4241
                     /gene="buk"
                     /locus_tag="KPE55_001351"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0492443.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4231..4908
                     /locus_tag="KPE55_001352"
     CDS             4231..4908
                     /locus_tag="KPE55_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0492444.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            4951..5166
                     /locus_tag="KPE55_001353"
     CDS             4951..5166
                     /locus_tag="KPE55_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0492445.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5195..6274
                     /locus_tag="KPE55_001354"
     CDS             5195..6274
                     /locus_tag="KPE55_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0492446.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6274..7026
                     /locus_tag="KPE55_001355"
     CDS             6274..7026
                     /locus_tag="KPE55_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0492447.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7027..7584
                     /locus_tag="KPE55_001356"
     CDS             7027..7584
                     /locus_tag="KPE55_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0492448.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            7754..9100
                     /locus_tag="KPE55_001357"
     CDS             7754..9100
                     /locus_tag="KPE55_001357"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG0492449.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9367..11199
                     /gene="glmS"
                     /locus_tag="KPE55_001358"
     CDS             9367..11199
                     /gene="glmS"
                     /locus_tag="KPE55_001358"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG0492450.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            11651..12946
                     /locus_tag="KPE55_001359"
     CDS             11651..12946
                     /locus_tag="KPE55_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG0492451.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVANYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGKESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13374..14111
                     /locus_tag="KPE55_001360"
     CDS             13374..14111
                     /locus_tag="KPE55_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG0492452.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14132..15385
                     /gene="murA"
                     /locus_tag="KPE55_001361"
     CDS             14132..15385
                     /gene="murA"
                     /locus_tag="KPE55_001361"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG0492453.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15472..16536
                     /gene="spoIID"
                     /locus_tag="KPE55_001362"
     CDS             15472..16536
                     /gene="spoIID"
                     /locus_tag="KPE55_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG0492454.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            16602..17270
                     /locus_tag="KPE55_001363"
     CDS             16602..17270
                     /locus_tag="KPE55_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG0492455.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17368..17628
                     /gene="spoIIID"
                     /locus_tag="KPE55_001364"
     CDS             17368..17628
                     /gene="spoIIID"
                     /locus_tag="KPE55_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG0492456.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            17825..18844
                     /locus_tag="KPE55_001365"
     CDS             17825..18844
                     /locus_tag="KPE55_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0492457.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            18860..19288
                     /gene="fabZ"
                     /locus_tag="KPE55_001366"
     CDS             18860..19288
                     /gene="fabZ"
                     /locus_tag="KPE55_001366"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG0492458.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19399..19908)
                     /gene="yyaC"
                     /locus_tag="KPE55_001367"
     CDS             complement(19399..19908)
                     /gene="yyaC"
                     /locus_tag="KPE55_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG0492459.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20097..20200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20337..21530
                     /locus_tag="KPE55_001368"
     CDS             20337..21530
                     /locus_tag="KPE55_001368"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG0492460.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            21846..22943
                     /locus_tag="KPE55_001369"
     CDS             21846..22943
                     /locus_tag="KPE55_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492461.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23207..25426
                     /locus_tag="KPE55_001370"
     CDS             23207..25426
                     /locus_tag="KPE55_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG0492462.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLIAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25429..26211
                     /locus_tag="KPE55_001371"
     CDS             25429..26211
                     /locus_tag="KPE55_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG0492463.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIICHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            26543..>27148
                     /locus_tag="KPE55_001372"
     CDS             26543..>27148
                     /locus_tag="KPE55_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0492464.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEE"
BASE COUNT        10884 a         2960 c         5221 g         8083 t
ORIGIN      
        1 gtagcgtata ttttttacaa aatatttatg tttataaaag atacaagagc agaacaagtg
       61 tttaaaggta taatatttct tttattagca actcaactta gtaacacctt taaattgcat
      121 accgtttatt ggatatctct taaagcttta gattatggag ttatagctgc tcttataatt
      181 tttcaaccag aatttagagc aggacttgaa catataggaa gagcaaagtt taatttattt
      241 ggaaagaatg taaatacttc tgaagaaact ctcaatagaa atatagaaga aattgtagaa
      301 gctttatatt cactttctag acaaaaaata ggagctctta tcataatgga gagagaaact
      361 agaataagtg atataataaa tacaggaact attattgatg ctgaaatttc taggcaacta
      421 ttaataaata tatttatacc aaatacacca ttacacgatg gagctgttgt aattagagat
      481 tctaaggtaa aagcagctgc atgttttttg ccattgacag aaagtaagga tttaagtaaa
      541 gatttgggca ctagacatag agcaggaata ggggtaagtg aggtgtctga ctgtataact
      601 ctgattgtat cagaggaaac aggtggcgta tcaattgcta aagcaggtaa gttatataga
      661 gatatttcta gagaaagaat gatgaatata ttacgtagta atttaaaaac aaatacagaa
      721 acaagaagtt ttttcaaagg tggtatattt aaatgataaa tagactaaaa aataatacca
      781 aaataaaaat aatatcattt ttaagtgcaa ttgcattatg gatgtatgtt atggctgtag
      841 ttgaccctga agaaactaaa gggtttgaag atatacctgt atctataagt aatatgagtg
      901 atttaaagga taaaaatctt gttatatatc cagacgaaaa attaacagca gatgtatata
      961 taaaaggtag tttatcaaag gtacaaagtg ttaaaaaaga agatatacat gtatatggaa
     1021 ccattgaaga cccaatacaa ggacagaagg aagtatattt aaaagcaaat attcctcaag
     1081 gtgttactct agaatttaag catgatgttt tagtagtaaa tcttgaaaaa aacataaaag
     1141 aaaaaagaga tataaaggta gaagtaagtg gaaattcaaa aagaaatata aataaaattg
     1201 aacttagtaa ggaatttgtc gatgtatcag gtccaagaac tctagttaaa gaagtcaaaa
     1261 gtgtaagagc taatctagat gttggtaatg agacagatga tttttctaaa aaattgcaat
     1321 taataccatt aaatagtgat ggaactaagg ttgaaggagt aacattaagt tcatcctttg
     1381 tgactgcaaa gatagagcta ttgaaggaaa aaattgtccc aataagagta agtctaaatg
     1441 ataataaaga taatgatgta ctgaaaaact ataaattaag taaggaacaa gttacaataa
     1501 aaggaaagaa agatgatata gacaaaataa gttatattaa gacaaaacct attgacttag
     1561 ctgatttggc taatggtgaa gaaaaagaag ttagcttaga aattccagag ggtataactc
     1621 ttgaggatag ctctataaca attaaattaa atgatacaaa acaattgagt gcagaatttt
     1681 tatataatgc gggagatatt gaattaagaa atatcccaac aaatatggat aatccaaatg
     1741 taatttctga tgtagatata aaagttgttg tagagtcaac ggaggatttg agtaagttaa
     1801 ataaaaaaga tatttcatta tatattgatt tatcacaagg tcctgaggaa gatagtaaat
     1861 ataaaataaa atatgaaaca acatatcaat ttaaaaaaat aaatatagaa cctaatattg
     1921 ttgaagtgga atgaacaaat atttcgaata tttagttgtt acaagatgcg acaaaaaaac
     1981 ctataaaatt aagtttgtta attttatgtt tttgttgcat cttgtttatt ttagttatat
     2041 tataaaataa ggacaatgag atatttgtta aatctaaaaa gcgctagata tatgaaaaaa
     2101 gactgataaa tagatttaaa taaatatttt gaattaaata tttcaattat ctctaggaaa
     2161 acgttgctga acgagttata atttaattga gatataaaaa gttctatttc aaaaaattca
     2221 agatgaggtg gaaatgcatg agaagttttg aagaagtaat taagtttgca aaagaaagag
     2281 gacctaaaac tatatcagta gcatgttgcc aagataaaga agttttaatg gcagttgaaa
     2341 tggctagaaa agaaaaaata gcaaatgcca ttttagtagg agatatagaa aagactaaag
     2401 aaattgcaaa aagcatagac atggatatcg aaaattatga actgatagat ataaaagatt
     2461 tagcagaagc atctctaaaa tctgttgaat tagtttcaca aggaaaagcc gacatggtaa
     2521 tgaaaggctt agtagacaca tcaataatac taaaagcagt tttaaataaa gaagtaggtc
     2581 ttagaactgg aaatgtatta agtcacgtag cagtatttga tgtagaggga tatgatagat
     2641 tatttttcgt aactgacgca gctatgaact tagctcctga tacaaatact aaaaagcaaa
     2701 tcatagaaaa tgcttgcaca gtagcacatt cattagatat aagtgaacca aaagttgctg
     2761 caatatgcgc aaaagaaaaa gtaaatccaa aaatgaaaga tacagttgaa gctaaagaac
     2821 tagaagaaat gtatgaaaga ggagaaatca aaggttgtat ggttggtgga ccttttgcaa
     2881 ttgataatgc agtatcttta gaagcagcta aacataaagg tataaatcat cctgtagcag
     2941 gaagagctga tatattatta gccccagata ttgaaggtgg taacatatta tataaagctt
     3001 tggtattctt ctcaaaatca aaaaatgcag gagttatagt tggggctaaa gcaccaataa
     3061 tattaacttc tagagcagac agtgaagaaa ctaaactaaa ctcaatagct ttaggtgttt
     3121 taatggcagc aaaggcataa ttttaagggg gaaataggca aatgagcaaa atatttaaaa
     3181 tcttaacaat aaatcctggt tcgacatcaa ctaaaatagc tgtatttgat aatgaggatt
     3241 tagtatttga aaaaacttta agacattctt cagaagaaat aggaaaatat gagaaggtgt
     3301 ctgaccaatt tgaatttcgt aaacaagtaa tagaagaagc tctaaaagaa ggtggagtaa
     3361 aaacatctga attagatgct gtagtaggta gaggaggact tcttaaacct ataaaaggtg
     3421 gtacttattc agtaagtgct gctatgattg aagatttaaa agtgggagtt ttaggagaac
     3481 acgcttcaaa cctaggtgga ataatagcaa aacaaatagg tgaagaagta aatgttcctt
     3541 catacatagt agaccctgtt gttgtagatg aattagaaga tgttgctaga atttctggta
     3601 tgcctgaaat aagtagagca agtgtagtac atgctttaaa tcaaaaggca atagcaagaa
     3661 gatatgctag agaaataaac aagaaatatg aagatataaa tcttatagtt gcacacatgg
     3721 gtggaggagt ttctgttgga gctcataaaa atggtaaaat agtagatgtt gcaaacgcat
     3781 tagatggaga aggacctttc tctccagaaa gaagtggtgg attaccagta ggtgcattag
     3841 taaaaatgtg ctttagtgga aaatatactc aagatgaaat taaaaagaaa ataaaaggta
     3901 atggcggatt agttgcatac ttaaacacta atgatgctag agaagttgaa gaaagaattg
     3961 aagctggtga tgaaaaagct aaattagtat atgaagctat ggcatatcaa atctctaaag
     4021 aaataggagc tagtgctgca gttcttaagg gagatgtaaa agcaatatta ttaactggtg
     4081 gaatcgcata ttcaaaaatg tttacagaaa tgattgcaga tagagttaaa tttatagcag
     4141 atgtaaaagt ttatccaggt gaagatgaaa tgattgcatt agctcaaggt ggacttagag
     4201 ttttaactgg tgaagaagag gctcaagttt atgataacta atgataaaag aataagaatc
     4261 ataactggtc actatggaag tggtaaaagt gaatttgcga tgaactatgt tgttaagtta
     4321 agagatatgg tttctggcaa agttgctata gccgatttag atgtggtaaa tgtatacttt
     4381 agaactagag aaaagaaaga gttaatgaaa tccttaggta tacaaccaat agatagctca
     4441 attaatgctc caacattaga tttaccagca gtatcagcag aggtaatgtc tcctatggta
     4501 gaccattcat acaattctgt tatagattta ggtggagata atgtaggtgc tagggttata
     4561 ggaagattta gtcatttgtt aaaagaaggc gattatgata tgctttttgt aataaatgca
     4621 aatagggaga aaactcaaac ttctgaagaa gttatccaat atataaaaga aatagaaaaa
     4681 tcttctaaat taaaggtaac aggtctaatt aataatactc atcttattag gtttactact
     4741 attgatgatg ttttaagagg tcagaaagtt gcaaaagaag tttctgaaaa atgtaatata
     4801 ccgataagat atgttgcatg tttagaaaat cttgttgaac agttaccaaa agatttagaa
     4861 ggagaaattt ttcctataaa attatatatg agagaagact ggatgtagtt tgagctcaaa
     4921 tatatagaaa gtttaaggag gcttaggtaa atggctaaag gaaaagtatc ttttaatcaa
     4981 gaacgttgta aaggttgtgg attgtgtgtt gaagcatgtc cagtaaaaat catacaatta
     5041 gactcaaatg taataaacaa aaaagggtat aacccagcaa ctgtttttga aatggaaaaa
     5101 tgcataggat gtgcaagttg tgcaactatg tgtccagatg tagttataac agtagaaaga
     5161 gattaattta attaatattt aagggggaat tttaatggct aagatactta tgaaaggtaa
     5221 cgaagcattt ggaaaagcag ctatagaagc tggttgcaaa tatttcttcg gttacccaat
     5281 aactccacaa agtgaattac cagaatattt atcaagagag ttacctaaaa taggtggagc
     5341 gttcgttcaa gctgaatcag aagtttctgc gataaacatg gtttatggtg gagcaggtgc
     5401 aggagcaaga gttatgactt cttcatcttc accaggagtt gctttaaagc aagaaggtat
     5461 aacatatgca gtaggagcag aagttccttg tgttgtactt aatgtaatga gaggtggtcc
     5521 aggacttgga agtatacaac cttcacaagc tgactacttt atgtctacaa gaggtggagg
     5581 aaatggagac tacagaactc cagtattcgc accagctaca gttcaagaag ctgttgatat
     5641 gataatggaa gcttttgacg ttgcagatta ttacagatca ccagttatgg tagttgcaga
     5701 tggtatgata ggtcaaatga tggagccagt tgaatttaga gctcctgaga agaaaagaga
     5761 attgcctcct aaagattggg caactgttgg aactaaagga aaaagaaaac ctaatgtaat
     5821 aaactctcta tacttagagc cagaagtact agaagaccat tgttggcatt tacaagaaaa
     5881 atttgatgca atggaaaaga atgaagtaca atacgaaatg tacaaaacag aagatgctga
     5941 atttgtattt gcagcttatg gaactacttc aagagtagta aaaagcgcaa ttgatatatt
     6001 aagagaagaa ggaataaaag caggtcttat aagacctaaa gtattatggc cattcccatt
     6061 cgaagcattc aaccaaattc ctaatgctag aaacatattg actgttgaaa tgagtatggg
     6121 acaaatggtg gaagatgtta aaatggcagt agaaggtaaa ttaccagttt acttccatgg
     6181 aagaccagga ggaatgactc caacaccagc agaaatagtt gaaaaagcta aaaaaattat
     6241 tgccggagaa ttggttgcag gaggtgctag ataatggctg ttgtatttaa gaaaactgaa
     6301 ggattacaag atactcaaac tcactactgt ccaggatgta cacatggtat aatccataga
     6361 ttagttggtg aagtattaga agaactagga gtattaggtg atgctgtagg tgttgttcca
     6421 gttggatgtt cagttttagg atataaatat tttaactgtg acactcaaga agctgctcat
     6481 ggtagagcgc cagctgctgc tacaggaata aaaagagttc atcctgaaaa tacagtattc
     6541 acttatcaag gtgatggaga cttagcttca attggtactg ctgaaatagt tcatgcagct
     6601 gctagaggag aaaaaataac tactatattt gtaaacaata caacttatgg tatgactggt
     6661 ggacaaatgg ctccaactac attagttgga caaagagcta ctactgctca atcaggaaga
     6721 aatgctgaaa ctcaaggtta tccaataaga gtaagtgaaa tgttagctac attaacagga
     6781 gctgtatttg tagaaagagt agcagttgat actccagctc atgttagaca agctaaaaaa
     6841 gctataaaga aagcattcca agttcaacaa gcaggattag gatttggaat agtagaagta
     6901 ttatctactt gtcctacaaa ctggggacta gctccaaatg atgctttaca atggttaaga
     6961 gacaacatga taccatacta tccattagga aatttcaaaa atgttgaagt tgaggaggtg
     7021 aagtaaatgt ctacagctag agtaatatgt gcaggcttcg gaggccaagg tgttatgtct
     7081 atgggacaat tacttactta tgcaggaatg ctagaaggaa aagaagtttc ttggttacca
     7141 tcttatggac cagaaatgcg tggaggtaca gcaaactgtg cagtaactgt atctaatgag
     7201 ccagttggtt ctccacttat aacagatgat gcaactgcag ctatattatt aaatatacca
     7261 gcatttgaaa aattcaaaga tgatgttatt cctggtggaa aaataatagt aaatagttct
     7321 ttaataaaag aaaaagttga tagaacagat gtagatgttt attatatacc agcaaatgaa
     7381 ttagcagctg aattaggaaa tgataaagtt gctaacatga taatgttagg cgcttactta
     7441 aaagtatctg atactgtaga tatagaatct gtactagaag cattcaaaaa agttttcgga
     7501 cctagaaaag aaaaattcgt tccattaaac agagaagctt tacaaaaagg tatggatgct
     7561 acatgttcta aagcaactaa ctaataaaga aaaagatgtc aaaaaaaaga caatagaatt
     7621 atttctattg tcttttttgc tgataagtat taaaatattt ttatgactga aattaagcct
     7681 ataatgatat tttatgaact ataaaaatat aatacaaatt acaagctttt caattatgaa
     7741 aggagaatct ttagtgagaa agtattttgg aacagatgga gttagaggag tagcaaatac
     7801 agaactaaca tgtgatttag catataaact tggtagagct ggtggatttg tcttagctca
     7861 aggagaccac agagtaaaag tagtagtagg taaggatact agaatatcag gtgatatgtt
     7921 ggaagcatct ttaatagcag gacttatgtc tgttggatgt gatgttatta ctgttggaat
     7981 aataccaaca cctgcagtgg catatttaac tagaaaatat ggtgctgatt gtggtgtggt
     8041 tatatctgca tctcacaatc ctgttgaata taatggaatc aaattcttta ataagaatgg
     8101 atataaatta gatgatgaaa ttgaattgaa aatagaagaa tatatagacg atatagacaa
     8161 aattgattgt cttccaattg gtgaaaatgt aggaagaaaa ttacatgaac attgtgcaca
     8221 aagagactat gtagactacc taaaatcaat aataagcact gactttaagg gattaaaagt
     8281 agttttggac tgtgctaatg gtgcgtcata taaagtggca ccaatagtgt ttgatgaact
     8341 aggagcaagt gtaatttcaa taaatagttc accagatgga aataatataa actataaatg
     8401 tggctctaca catccagaac aacttcaaag ggctgttttg gaacataatg ctgacttagg
     8461 tcttgcatat gatggagatg ctgatagatt aattgcagta aatgaaaagg gtcaaattgt
     8521 tgatggagac catataatga tattgagtgc cttaaattta aagaaaaata ataaattggc
     8581 acaagatact ttggtagtta cagttatgag taatataggg ttgacaatag ctgctaagga
     8641 aaatggaata aatttatcta ctacagcagt tggagacaga tatgttttag aagatatggt
     8701 taaaaatgga tataatcttg gtggagagca atctggacat atgatatttt tagattacaa
     8761 cactactgga gatggagtgc ttagttctct aatacttgca aatataatct tgcaagaaaa
     8821 gaaaccttta tcagaaattg catcaataat gagccaatac ccacaagtat tagtaaatgc
     8881 aacaataaaa aatgaaaata agaacaaata tatggaatat ccagaaataa aaactgagat
     8941 agaaagaata gaatctattt tagacggaaa tggtagagtt ttgataagac catcaggaac
     9001 tgagccatta gttagagtta tgttagaggg taaagaggaa ggtcaaataa aagaattagc
     9061 aacaaactta gctaatctta tacaagaaaa attatcgtaa tttaaagggt attctcagat
     9121 ataaatactg aggacacctt tctaagcgcc agaacttagt tatagacata attattgagt
     9181 aatataggaa ctaagttgac gaggtcagag ttaatcgaat tgtcggcgga tgctctgcgg
     9241 tgtgtttacc atcgaaagac ttctttaaat aataggagca atcctatgga caaagggaag
     9301 ccaaagtaaa cttatatgac ctaactctta aaatatataa ataatatttt taggaggaca
     9361 caaataatgt gtggaatagt tggatattta ggaagtagaa aggcagcaga agttatagta
     9421 gaagggcttt ctaagctaga gtacagaggt tatgactcag caggagtagc agttaatagt
     9481 tctaatgaaa aagaacttaa tattagaaag tttaaaggta gactttctgt acttgcagaa
     9541 gacttagaaa agaatcctat tgatggaaat ttaggtatag ggcatacgag atgggcaact
     9601 catggagaac catctgatgt aaactcacat cctcatttta atcaagccaa aacaatagca
     9661 gttgttcata atgggataat agaaaactat atggaaataa aagaagaatt aatttctgaa
     9721 ggtgttaagt ttgaatctca aacagataca gaggtaatag cacatttagt agataaatac
     9781 tatgagggta atttattaga tgctgtatac aaaactatat caaaattaag aggagcttat
     9841 gcattaggtg ttatatgtaa agaacatgga aatgaattag ttgcagtaag aaaagatagt
     9901 cctcttgtag ttggtgttgg tgaaggtgaa aactttatag catcagatat acctgcatta
     9961 ttaaaatata ctagagatgt ttatttctta gaaaatggag aagtagttca tctaaaagac
    10021 gaaaatgtaa cagtttatga ctctaataga aatcttgttg aaaaagaagt atttcatgta
    10081 acttgggatg tagaagctgc gtctaaaggt ggatatgact actttatgtc aaaagaaatt
    10141 catgagcaac caacaggagt aagagaaact ttagaaagaa gacttgatga taatggaaat
    10201 ataattctag acagtataaa tatttctaaa gaagacttag aaaaaataaa taaagtttat
    10261 atagtggctt gtggaacagc atataatgct ggattacttg gtaagtatgc catagaaaaa
    10321 tttgtaaata taccagtaat aactgatata gcatcagaat ttagatatag cgacccattt
    10381 gtagatgaaa attcattagt aatattagtt agtcaatctg gtgagacagc agatactcta
    10441 gcagtattaa gagattcaaa ggctaaagga gcaagaatac tttcaataac taacgttgta
    10501 ggttcatcta tagcaagaga atcagatgat gtattctata cttgggcagg acctgaagta
    10561 gcagttgcat caactaaggc atatactact caaataactt ctttatacat gatagcttta
    10621 gactttgcta tcaaaaaagg aacaataact agagaatttt atgatagtat gatttctaag
    10681 atgaaagaaa taccatcaaa aatacaagaa atactagaca atgaagaata tataaaagaa
    10741 gttgctaaaa cagttgttag ttctgaacat gcattctatt taggaagagg tatagactat
    10801 agcttagcaa tggaaggttc attgaaatta aaagagattt catatataca tgcagaagct
    10861 tttgcagcag gagagctaaa gcatggaaca atagctttaa tagaaaaagg aactcctgta
    10921 attgccatag ctactcaaga aaagttattt gaaaaaatgg tttctaatat ggaagaagta
    10981 agagcaagag gagcttatgt agtagcaata gctcaaagcc ataataagga tgttgagaaa
    11041 gctgctgata aaataatcta tataccaaat tcagatgata ttttatcacc aatattagca
    11101 gttgtaccaa tgcaattact tgcttatcat gtctctgtac taagaggatg cgatgtagat
    11161 aagccaagaa atctagctaa atcagtaaca gttgaataaa aacagattaa aaattaaata
    11221 gataatattt attgaaaaga actcatcctg ttatttgtat tttataggat gagtttttta
    11281 gataaatatt aaatttgttt tgtttttgct ataataaatt ttataaaaag attgatatta
    11341 aatcatctgt aaaatgataa atatatagac cataccaggt atagggaacg ataaatatag
    11401 ttcatataaa atatagtgag tacttaataa acatatagtc aatatcagat aaaaaaaata
    11461 atagcttatg agaaaaatta aaatacaagg tattagtaac cttgtgataa gataaagtga
    11521 gtagtttaaa ataagaatat aaggaactat ttataaaaga atataatgaa gctaatagag
    11581 tataagaaaa ttactttatt ttagtaaaat aatttcaaat aaaaattttt ttaaattggg
    11641 agtataaatt atgacaaatg attttaaaaa agaaaagaaa gaagattatt ttgttaatct
    11701 taaggcaata tctactgaag ttttacaaga aaaagtgcaa gataacgctt gggttatagt
    11761 tgatactcgc ttaaatgatg cttataatgg ctggaagtta gatggagtaa aaagaggtgg
    11821 acatataaaa ggagcagtag atttttctgc taattggtta agtgtatact cagatagaaa
    11881 agatgaagtg ttagaacaag cattgaaaac taaaagaatt gatttagata aaaatatagt
    11941 attgtatgat gcaaatggaa aagatgcttt ggttgtagca aattatttat ctaaaaaagg
    12001 atataaatat ctatataaat atgatattaa acaatgggca gacgacgaaa atcttccaat
    12061 ggaaagatat aaaaattatc aaatgatagt tcctgctttt ataataaaag atatattaga
    12121 tggaaaaatt cctgagacat ttgaagattc aaagaatata aaaatgattg aagcgagttg
    12181 gggaaaagaa tcgtatacaa aagggcatat acctacaagt gttcatgtaa atactgatat
    12241 tatagaacct cctccaacat ggatgcttga taatgatgat aacttaacta agtttgcttt
    12301 agactatgga ttaacaaagg atgatactgt aatagtttca agtagtacac ctatggcatc
    12361 ttatagacta gctgttatat tgagatatat aggagtaaaa gatgtaagag ttttgaatgg
    12421 tggaacaaac tcatggttat cagcaggata tgaattagaa tttattagta atccaaaaca
    12481 ttcatgtact aattttggag cagatatacc agttaactca caattaattg ttactacatc
    12541 tgaattaaga caaaaactaa aagaaaaaaa taagtttata ctagtagata accgtacttg
    12601 ggatgaacat ataggaaaag tatctggata tacatattat gataaaaaag ggcgtatacc
    12661 aggagcttta tatggacatt caggtagtga tagtgtttca ctagaagaat atcgtaatat
    12721 tgataataca atgagaaata aatatgagat tttagaaatg tgggacaagg aaaatataga
    12781 tgtgaataaa cagcttatat ttatgtgtgg aagtggatgg cgtgctgctg aagtattaac
    12841 atatgcaaat gtcataggag tggaaaatac ctccctttat agtgatggat ggatgggctg
    12901 gagtcttgat aattcaaatt taattgaagt gggagaacac aaataaagtt tttgaaagtg
    12961 caatagagtt gtttaaaaca aaggtgtttt tgatttatta ggaagagtgt gtaaattgtg
    13021 tattgggaag tgtacttagg tatgttcatt tggagctatg gaaattgaca aaaaagtatt
    13081 atctgtttat tttgttggta aaatatgaat agattataga tttaagtata gactaagtaa
    13141 tttatattac agaagagaat atataagaat taatgcaaga tgtatttgat acatataaga
    13201 aattggcaag tccaggagaa ggatagagaa aattgagatt gaagagtttg aaagagatta
    13261 ttagatgaaa aatacaaaaa tgtataaaaa tacatattga gaaagtatat ctctcttaaa
    13321 tttgttaata attctattat acagaaaaac atatttaagg gggatttttt actatgaaaa
    13381 aagtaaaatt agctataagc ttttttatat taatgattat aggaatgaca atatcttatg
    13441 cagatacaag tataaatact agatacaata gattaagtaa agctcttgaa atatcagaag
    13501 caaattttaa attttataat gttaaaatta attctgttat aaatcataat ttatcaaata
    13561 aagaatttca taatattttt atagaaataa ttaacaatct aaagttggat aaaaaaggaa
    13621 taaattggaa tcaaaaaggg tctggcgata aaatacaatt atgtgctact ataaaaggta
    13681 gcgataaaga aatttctata aaaggaataa ataaaaataa caaagaaagt tacatagtag
    13741 ttgacatatt ggataacaaa gtatataaac ataaagaggt tatttatagt aacgtagaaa
    13801 atgtgttaaa taaatattca tctcaagtag atgtgtctgc ttgtattgtt ggagaataca
    13861 caaaaaggtt acaaaacaac aaatatgatg atattttaca aaaaatatta tataatatga
    13921 gtgctaagca aatagatgaa gttaaagacg taaacttttt atctataaca gcatatagta
    13981 aagtattaaa tgattatgaa ttagaatatt taggggaaaa aattaattta aatataggaa
    14041 ttaaatataa tgaggatgac ggaaaaacac tcatatatat tgccacacca attataaaat
    14101 tagattatta attatgagga gatcaaaaaa tatggctaag ataatagtaa aaaagagtaa
    14161 tccacttaaa ggtagtgtta aaatagatgg tgcaaaaaat gcagttttac caataatagc
    14221 agcgacttta ttagcaaacg gaaaatcaac attaaatgga gtaccaaatt taagagatgt
    14281 tcatgttata tcagatttat taagacatgt aggagcagaa gttgaatata aagaaaatac
    14341 acttactgtt gatgcaagca atataaaaac ttgtgaagct ccatatgagc ttgtaagaaa
    14401 gatgagagct tcttttttgg ttatgggacc attgcttgct agatttaata gtacgaaaat
    14461 ttctatgcca ggaggatgtg ctataggaac aagacctata gatttacatt taaaaggatt
    14521 taaagcctta ggtgcaaaga tagaaatgga ccatggtttt gttgaagcag caacagaaaa
    14581 gttagtagga aataaattat atttagattt cccatctgtt ggagcaactg aaaacataat
    14641 gatggcagct tctttagcag aagggactac aataatagag aatgcagcag aagagccaga
    14701 aatagtagat ttagcaaact tcttgaacga aatgggagca gatgttaaag gtgcaggtac
    14761 taatactata aaaataaaag gtgttaaaga gttaaaagga gcagaacata atgtaattcc
    14821 agacagaata gaagcagcaa cttatatggt tgcagcagct atgacaaaag gtgatataac
    14881 tgttgaaaat gttttaatgg agcatttaaa accagttgta gctaaattaa gagaagctgg
    14941 ttgtgaaata acagaaatgg acaattctgt aagagttgta ggtccaaaag tattaaaacc
    15001 aatagatata aaaactttac cacatccagg atttcctaca gatgttcaag cacaatttat
    15061 ggctatgctt actgtagcca atggaactgg tgtagttata gaaactgttt ttgaaaatag
    15121 atttatgcat gttgctgaat ttaatagaat gggtgctaat ataaaaatag atggaagaag
    15181 tgcggttgtt aatggtgtag atgagctaca cggtgcagca gtaaacgcta ctgatttaag
    15241 agcaggggca gcccttattt tatgcggtct tatagcagaa ggagaaactc aaataggaga
    15301 aatataccat atccaaagag gttatgttga tatagataag aagataacag cactaggtgg
    15361 tcaaatcgaa atagtagaag attaataatt ggtttataga agaaagttat aaattaatag
    15421 ggttgttcat atacttaaat acaaggtttt aaaagctaag gaggagagta tatgaagaac
    15481 ccattggttg ttttgttagg atttgtgact tgctctgtgt tagttccatc acttataact
    15541 ttagtttctt ataaaaatgt agaattaact gaaaaaccag agagcccagt atctataaac
    15601 aaaactatta aaaaaagtga tattgaagac aaaggtaata aagaagaaaa aaatacagtg
    15661 aactatgaaa ctgtaaataa aaaagcacca attatcaatg tatataatca tataacaggg
    15721 aaaaccgaaa agatggacat ggagaactat ttatgtggag tactagctgg tgagatgtct
    15781 tcagagtttg atatagaagc tttaaaagct caatctgtag cagctagaac atatgtagta
    15841 tataaacagg aacatggtaa atctagtaaa cataaaaatg cagtagtatg tactgattat
    15901 aaacattgtc aagaatataa gagttatgat actcttaaaa aattaaatgg tgaagaatgg
    15961 attaagaata aatattccaa aatccaagaa gcagttagag gaactaaagg tcaaataata
    16021 acttataatg ataaggcgat acttcctctt tatttttcta catcttcagg aaaaacagaa
    16081 aatagtgaag aagtattttc tgcaaaatat ccttatttaa agtctgtgga aagtccttat
    16141 gataagtatt cccctaaatt tgcgtcaaca cttaaaataa gtaacacaga ttttgttaaa
    16201 agtcttagaa gggcatatag cactattgtg atagatgtga ataatttaag taaacaagtt
    16261 tcaataacta aaagaagtga tgccggaact gtagaaaaaa taaaattagg aaataaagaa
    16321 ttgactggaa aagacattag aactgttttt aaattgaact ctgcaaactt tgacataaaa
    16381 tttggagaag gatacataga tttcgtggta aaaggatatg gtcatggtgt tggtatgagc
    16441 cagtggggag cagaaggaat ggcagaagag ggctataaat attatgatat attatcacat
    16501 tactacacag atacaaaaat aaaagatata tactaataat tgtataaaat gccaaaaaca
    16561 cgtcaataat ccaaatgtag aaatatattt ggaggtgcaa aatgaagaaa aagctgttag
    16621 aaaaagatgg tttttattta tctttgtttg tatgtgtttg tttattagca gtaggtggag
    16681 tttggtttac aaataataat gtagataaat tggcttctaa taaaggaatt atggaaaatg
    16741 ctaataaaga tagtgaagag gaaatacatt taattgaaaa agacaagaaa gatgctatcc
    16801 ctactgcaac ggattcaaaa caaaacttag aaaaagctaa gtcaaaagag gaaaataaat
    16861 cgagtacaac aaaattaaat tacattggag ataaagtaat aagaggatat tcagaaaaag
    16921 agcctagtta ttctaaaaca cttgatgttt gggaaactca taaaggtgta gatattagtt
    16981 gtactaaagg caaagaagta aaatctctat taaacggaat agtagtagat gtatttgatg
    17041 atgaagaata tggacaatca gtcaaaataa aaagtgacaa taacattgta gtagtatatt
    17101 caaatctaga taaaaatgtt agtgttaaga aagaacagaa ggtaacagaa ggacaatctc
    17161 taggtactgt aggtagtact tctcaaatag aaagtgaaga aggtatacat gtgcatttag
    17221 aggcgtatag tggagaaaaa tctatagacc caatgagtct aattaagtaa tagtataaca
    17281 agtttaatca tatttggtta agcttgttat attttttggt gtttttacat atatatcatt
    17341 aaagctatta tgtgaggggg gtaatctttg agatctcata tagaggaaag ggccatagtg
    17401 gtagcaaaat atatacttga aaaaaacact acagtaagac aaaccgccaa gacatttgga
    17461 gtaagtaaaa gtacaattca taaggatgtt acagaaagat tgaaggaaat aaatccatcc
    17521 cttgccaaag aagttaaaaa tgtgctggac aaaaataaat cagagagaca tattagaggg
    17581 ggattagcaa cgaaattaaa atatgagaag gaacataaaa aaatgtaggt taaaaaccta
    17641 ctttttttat tgaattaaat tataaaaaga aatatattaa gtaattataa atagcaaaat
    17701 aataatactc tcaaaagatt tattttctct tgattaaagc gagatttttg tatatgcttt
    17761 attataggag tattaaaata ggcttataat ataatctaat attataaata aaggagttga
    17821 aaaaatggct ggagctgata taggcataga tttaggtact gcaaatgtgc ttgtttatgt
    17881 tagtggtaag ggaatcgttt tagaagaacc atctgttgtg gcgatagaca aaagaacaga
    17941 ttctgtttta gctgtgggag aagaagctaa aaagatgata gggagaacac caggtaatat
    18001 agtcgctata agacctttaa gagatggtgt aatttcagat tatgatgtaa ctgagaagat
    18061 gttaaaatct tttatagata agatagttga taaaaaggga tttggaagat tttttatgcc
    18121 aagaattatg gtatgtgtac caacaggtgt cacagaagtg gaaaaaagag cagtagaaga
    18181 tgctacgaga caagctggag ctagagaagt atatatcata gaagaaccaa tagcagctgc
    18241 cataggagct ggtgtagaca tatcacagcc taatggtaat atgattatag atattggtgg
    18301 aggtactgta gatatagctg taatatctct aggaggagct gttgtaagtg aatctataaa
    18361 ggttggtgga gatagatttg atgatgctat agttaaatat atgaaaaaac aacacaatct
    18421 tcttatagga gaaagaacag cagagaaaat caagtttgaa ataggttctg catttaagag
    18481 agaagaagaa aaatatatga agataacagg aagaaattta attacaggac ttcctagttc
    18541 tataactata aattcaacag aaatgttgga agcacttaga gaatgtgttg agcaaatagt
    18601 agttgctact catgctgttt tggaaaaaac gcctcctgag ttagcagcag atataggaga
    18661 tgtcggtata ataatgactg gaggaggttc attattatat ggattagata aaataataga
    18721 acagaggaca ggaataactg ttacaatagc agatgaacca ttatcttgtg ttgcaaaagg
    18781 tacaggaata gctttaggtt caatagactt acttgaaact ggtgggtctt tcaaaagaaa
    18841 ataattagga gagtttagaa tgttaaatat agatgaaata aaaaaattaa taccacatag
    18901 atatccattt ttattagtag acaagattac agagctagaa gttgggaaaa gagctgttgg
    18961 tataaagaat gtaacagtta atgagccatt tttccaaggt cactttccag aatatccttt
    19021 gatgccagga gtactaatag tagaagctct agcacaagta tgtggagtgg caatgatgtc
    19081 tgtagaggaa aacaaaggaa aactaggtgt atttgcaggt atagataaag ttagaatcaa
    19141 gagagaagta agaccaggag atacattaac tatggaagtt gaaatgacaa ctcttagaaa
    19201 aaatatagct aaggcagatg ctaaggcata tgtaggtgaa gaacttgtct gtaaaggtga
    19261 attaatgttt gctttagtag aaaaataaaa acttgaaata taaaagtaaa aaggtagata
    19321 atttggagta tttaatcaga tactctaaaa tatctacctt tttatataaa gaattttatc
    19381 atttagttta tatttagatt aggtggatac taataaagcc aaggctattg tttcagctaa
    19441 atctaatata aagctaaggc gtatattatt aaatgaaagt ttattatttt catcaatttt
    19501 atctacaatt cctactatag aataatttcc tatttgaggt agttttttac caacaccttt
    19561 tccgggcagg ataggtcctt ctctaatctg tatattacct atgttactct gcgaccccaa
    19621 gcaagcatct atagccaaga agttaccttg tatatgtgta ttttttatag tatctaaaga
    19681 ctcatatata tttaaggcgt gaatagggtt atctagtgta ccgtaaacgg ggtatttaaa
    19741 gttactgttc ttaagtattg ttccaactaa agggcctaaa gtatcaccta tagccctgtc
    19801 tgttcctatg caaacaacaa tcgtattttc attaattata gtttttagaa ctgaacttag
    19861 catctgtata acatcatcat cgctgtagtt tactttagct aaatacaata ggaacacccc
    19921 cttcaaaaaa atatatgaat aaaaagcatt aaaaagacct aactttaaat ttcagataga
    19981 tagaaaattt tatttgtctt acaaaaaaag gaatagaaaa aatatgacta aaaatattga
    20041 caaaatttta aaaaaatagt aagctagtaa agtaaaacaa aacttaaatc ttgattacct
    20101 tatcaagaga ggctgaggga taggccctgt gaagcccagc aaccacccaa atgggaaaag
    20161 gtgctaattc ctacaagaca aaagtcttga aagatgaggt cagttaatta gtatgtaatt
    20221 acgattatta acctcttctt ataaaggaga ggttttttta ttttgagtaa atttctaaaa
    20281 ttaaaaataa ggtaaaacct ataataaaaa ttaagaataa ataaggggga ataaaaatgg
    20341 caagacattt atttacgtca gaatcagtta cagaaggaca tcctgataag atatgtgacc
    20401 aaatatcaga ttcaatatta gatgcattat tagaaaaaga cccacaatct agagtagctt
    20461 gtgaaactac agttactact ggtttagttt tagtagcagg agaaataagt acatctgctt
    20521 atgtagacat tcctaaatta gtaagagaaa cagtaagaga aattggatat acaagagcaa
    20581 aatatggatt tgattgtgat acttgcgcag ttataacttc tattgatgaa caatctggag
    20641 atatagctat gggagttgat gaaggcctag aaagtaagac tggagaagag atagaagaag
    20701 aaatagaaaa agttggagct ggagaccaag gtataatgtt tggatttgca tgtaatgaga
    20761 ctccagaatt aatgccactt ccgatatctt tggcacataa attatcaaga agacttacag
    20821 aagttagaaa aacaggttta gttgattatt taagaccaga tggaaaaact caagttactg
    20881 ttgaatatga aggaagtaaa gctgtaagag tacatacagt tcttatatca gctcaacatt
    20941 gtgaaacagt atcaaatgat aaaataagag aagatttaat taatcatgtt attaaggaag
    21001 ttataccagc agaattactt gatgaagaga ctaaaattta tataaatcca acaggaagat
    21061 tcgttatagg aggaccacaa ggagatacag gtcttacagg aagaaaaata ataatagata
    21121 cttatggtgg atattctaga catggtggag gagctttctc aggaaaagac cctacaaaag
    21181 ttgatagatc tgctgcttac gcagctagat atgttgcaaa aaatattgtt gcggctggtc
    21241 ttgcagacaa atgtgagata gaacttgcat atgctatagg tatagctaga ccattatcta
    21301 tatttataga tacttttggg acaggcaagg tttcagaaga aaaacttgtt gaattagtaa
    21361 ataaacactt tgatttaaga ccaggagcga taataagaga cttagattta agaaagcctc
    21421 tttataaaaa ggttgctgct tatggtcact ttggtagaac tgatatagat ttaccttggg
    21481 aaagaactga caaggttgag cagttaagaa aagatgcttt aggtgaataa tttttaacta
    21541 aaaagctgtt agaatgtaat tttacgatta ctttctaaca gcttttttat tatgagttaa
    21601 atatttttaa taaatgttaa tttatcatca tataatgaat aaacagtatt taatctggtc
    21661 aatacatttt atataatact ctactaaatt tatacatatt atgggatata tgttaaagga
    21721 tggttaagaa tgtgttaact aatgttaaat aattaaaaat atattgaaat atcacaataa
    21781 tgagtctaat ataatcttaa taaacttaca ctttaaaaat aattttaatt gggggggagt
    21841 agtgtatgtc aggcaaagta aatattaaaa cggttataag ttttgctgga gcttatgttg
    21901 ctactgtaat aggttctgga tttgcaactg gtcaagaaat actgcaattt tttacgtttt
    21961 atggatatgc gggtattata ggtggaataa tctctatggt actattttca tggtttggag
    22021 cagaagttat agataaggga agagaattga aacttaagga gccaataaaa atttatcaag
    22081 tatattgtgg aaaatatctt ggaacatttt ttgaatggtt tggtccattg tttttatttg
    22141 gaacattagt aataatgatt gcaggagcag gagctacatt atcagagtac tatggattaa
    22201 atccatatgt aggtaggatt ggtatggcaa tcgtttccct aataactgtt tcattaggtt
    22261 tgacaaggct ttctaaaata ctaggtaata taggacctat aataataata tttacacttt
    22321 tagtaggagc aattagttta tttagcaata ttgatgcttt atcaaatgct ggaaatatgg
    22381 ttgatagttt aaatattaaa actgctacat caaatggata tttctcggga gtactttata
    22441 cttgttacaa tgtaataata gtaataacat tcttgacagg aatgggagct tctgcagtaa
    22501 ataagaaaga tgctgtatgg ggtggtatag taggtggagt agcacttatg gcagcagcta
    22561 taatgatgaa tttggcatta ctttcagaca taggaaatat atacacaaaa gagataccag
    22621 cactttactt agctgataag atatctccaa taataggtat tttattctca gtagtacttt
    22681 tactaggaat atacactaca gctgtaccat tgttatggtc agtaacaaac cgatttgtag
    22741 aagatgacca ccctaagttt aaaattataa caatagtggt aagtatatta gcttgtatag
    22801 gaggtttact gccatttgat aagttagtag gtacactata tccatataca ggttacatgg
    22861 gaattttaat tttactatgt atgctttata gaagaattac taaaactgag gggtacaagg
    22921 agaataaatc tgaaataagt tagtactgta atatacttat gaaatttaat ttatactcta
    22981 aatatagagt aattaaaata aaatagctat ttaaatatgg tacctgtatt tcatggtacc
    23041 atattttatg gatgcagatt ttttttgttt ctgataaaat gtataatagt tggaaattac
    23101 gttaagtata aaaatataaa acttttctac gattatcata gttttaatat ataaaattat
    23161 actaacattt aaatggagcg attattagta tttttaggag ataaaaatgg aaaaattaga
    23221 aggaatgata agtgaaatag tctttaaaaa cgaagataat ggttatacaa tagctcacct
    23281 ggtaaatgaa aatgatgaaa ttgtggttgt aggatgtatg cctactttgg caattggaga
    23341 aagcatagaa gttgaaggaa agtgggtaaa tcataagatt tatgggactc aatttgaagt
    23401 gaatagtttt atgccagtaa caccatcatc cttagaagga atttatgttt acttatcttc
    23461 tggaatgata catggtattg gtgaaaaaat ggcaaaaaga ataattgata agtttggtgt
    23521 agatacgtta gaggtgatac aaaattcacc agaaaaacta caagaagtag aaggcatagg
    23581 gagtaaaaaa gtaaaacaaa tagtaaaaag ttatgaagaa gacagagaat tgagaaatat
    23641 aataatacag ctatctcctt ttggaataac accaaattac tgcctaaaaa tatataaaaa
    23701 atataaaagc agtgcaatag aagttataaa taaaaaccca taccaacttg ctgaagatat
    23761 aagaggaatt ggatttaaag tagctgatag tatagcaagt aaaattggta tagataaaaa
    23821 ctctaaagat agaatttgtc agggaatctt atacacttta aataaatctt taagtaatgg
    23881 gcatacatat ttaccagagc atgttctaat acaagattcg gaaaaattat tggagctaaa
    23941 tggagaaata ataaaagagt gtgtaatgat gctagtttat aatcaaaaaa tacatataga
    24001 gaaagtaaac aatgaaaatc ttatatacct tatgccatac tatttagctg aaaatggagt
    24061 atgtagtcaa atagtaaaat tatcacagta tgaatttgaa gacttaaaaa tagatataga
    24121 taacgagatt aatgttttag aagaagataa gaaaattaag ttagcagaaa aacaaatttt
    24181 agcagtcaaa gaatctgtta atagtggagt attaattata actggtggac caggaactgg
    24241 taaaacaact accattaatg ctatcataga tatatttgaa aataatggta aaagtgtgac
    24301 tttggcagct ccaacgggaa gagcagcaaa gagaatgagt gaaacttcta ataaagaagc
    24361 aaaaactata cataggttgc tagaaatggg attttcaaca gatgatgatt taactttttt
    24421 taaggatgaa gaagacccaa taaattcaga tgtaataatt gttgatgagg tatcaatggt
    24481 agacattatc ttaatgtaca atttacttat agctataaag ttaggaacaa gagtaatttt
    24541 agttggagat agtgaccaat taccttctgt aggtgctgga aatgtactta aggatatgat
    24601 taattctaat attataaatg tagttaaatt aaatgaaata tttagacaag cgcaagaaag
    24661 tatgataatt gtaaatgccc ataagataaa caatggagaa ccactttatc taaatacaaa
    24721 aggtaaagat ttctttttta taagaaaatc aactaatgaa gaaattttaa atgaaataat
    24781 aggattggtt aatgaaagat tacctaaatt ttataaagtt gataaattaa aagatataca
    24841 agttttatct tcaatgagaa aaggtgaatt gggagttact aatctcaata tagagttaca
    24901 gaagtatctt aacaaaaaag aaaaatttaa agttgaagaa agtttttcta aaagattatt
    24961 tagagttggc gataaggtaa tgcaagtaaa aaataattat acaaaaaaat gggaaacaga
    25021 agaccaaaaa gaaagtggag aaggtatcta taatggagat ataggatatg tttatcacat
    25081 agataaagat aagaaaacta tttatatatt atttgaccag actaaaattg tatcatacct
    25141 atatgatgaa ttagatgaaa ttgaccatag tttttgtaca acaatacata aaagccaggg
    25201 aagtgaattt ccagttgtag tattgccaat agcgtgggct cctcctatgt tacttagccg
    25261 taatttatta tatacagcag taacaagagc aaaaaaatta gttgtattag ttggagatgt
    25321 gaaatatctt gaatatatga taaaaaataa tagagttaat caaaggtatt caaatttagg
    25381 atataagcta aataaattta aacaagaagg gttgttgata gaataaatat gaaaatatgc
    25441 aatgataatt ttattaagaa ttttaaaaat atgattaata aatgtttgga ttttatatat
    25501 cctgagaata taagttgcat aatttgtgat aaatccataa agaaaacaaa tacttattca
    25561 atatgtaaaa gttgttttaa agaaatgaat tttatacaag atggttgcat gaagtgtgga
    25621 aaaccaataa tatgtcattc aattgaaaaa gaatttattg aagagtgtag ttattgtttt
    25681 aataaagact tttattttga taaatcaata tcttgtattg aatacaatga tgtgacaaaa
    25741 aagatggtgt tagggctgaa gtataaccaa aaaaccttta tggctaagta tatagctcaa
    25801 ataatgaaag aaaaattata tttagaaaac ataaaatttg attatatatt atttgttcca
    25861 cttcataaaa aaaggctaaa taaaagaggt tttaatcagg cacaaaaaat agcatttaat
    25921 ttaagtaaaa tagtaaatat tccattatta gattgcataa gtagaaaaaa atatacaaga
    25981 atgctatata aattgaataa gaaagaacga aaagaagaat taaaaaatgt atttgttgtt
    26041 aaagaaaatg ttaaattaat aaataataaa aatatacttt taatagatga tatatttact
    26101 actggtctaa caacaaacga aatttctaaa cttttaaagt tatcaggtgc taataaggtt
    26161 tttgtattga ctctacttac aaaagcaaat gataattata ttatggaata atcttatata
    26221 ttgacaattt tagtttaaaa tattaaaatg aataagtgat atttaggtct gtggttgaaa
    26281 gtccaagcca gtcgcaggca aagggatcca cgtaagttaa cactaaaaat tgggttaatg
    26341 atcatggtgc ggcttagaag taagacctac cgataataaa atcgagagag agtagtagtg
    26401 cggcgaaccc aagcgaaccc tccagtaggc gagtgtgggg tcaaaaacca ggtcagctaa
    26461 atatcatatt aaaagagagc tagcatacct ttatgtatag ctctttttta tatataataa
    26521 atttaggttg agtcataaag aggtgatatg tttgaataaa agacaagaaa atttattaac
    26581 aacattgcta ttaaaagaag agtttataaa tgttaataag attgccaatg attttgagtg
    26641 ttcagaaagg acaataagaa atgattgcaa atatattgat gaatggttat ctactttttg
    26701 taatgcttat atcaaaagaa aacctaatat tgggattaaa tttagtggag atggaacaga
    26761 caaaatactg gtaaatcaga agttgcgagg tgaagttaaa agagtaaata gtgagcttca
    26821 taagcaagtt gagatattaa atttactact ttgtaataat aaaaaagtta ctttaaatga
    26881 tttatctaat aagttgtaca taaataaaaa tattgtcaga gaagaaataa gtaaattatc
    26941 agtagcatta aataattata atttaaatat ctcgactaaa aaaggcagtg gtatatatat
    27001 tgaaggggat gagaaagaca taagaataat gttagtatca ttcttattta aatatataga
    27061 taaatacaga ttaaacatag atgaaataga gtacttccat atagtagata taataattgt
    27121 aaaaaacata atgcttacta tagaggag
//
LOCUS       DAELNY010000038        26150 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.038, whole genome shotgun sequence.
ACCESSION   DAELNY010000038 DAELNY010000000
VERSION     DAELNY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.038"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            45..1850
                     /locus_tag="KPE55_001373"
     CDS             45..1850
                     /locus_tag="KPE55_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0492465.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            1927..2465
                     /locus_tag="KPE55_001374"
                     /pseudo
     CDS             1927..2465
                     /locus_tag="KPE55_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            3079..4293
                     /gene="dpaL"
                     /locus_tag="KPE55_001375"
     CDS             3079..4293
                     /gene="dpaL"
                     /locus_tag="KPE55_001375"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0492466.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAK
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            4558..5745
                     /locus_tag="KPE55_001376"
     CDS             4558..5745
                     /locus_tag="KPE55_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG0492467.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGTEGYYGILKSV
                     YKK"
     gene            5761..7344
                     /locus_tag="KPE55_001377"
     CDS             5761..7344
                     /locus_tag="KPE55_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0492468.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAARSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            7372..8700
                     /gene="ssnA"
                     /locus_tag="KPE55_001378"
     CDS             7372..8700
                     /gene="ssnA"
                     /locus_tag="KPE55_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG0492469.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKVKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            8845..10197
                     /locus_tag="KPE55_001379"
     CDS             8845..10197
                     /locus_tag="KPE55_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0492470.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            10389..11498
                     /locus_tag="KPE55_001380"
     CDS             10389..11498
                     /locus_tag="KPE55_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0492471.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMLMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            11781..13172
                     /gene="hydA"
                     /locus_tag="KPE55_001381"
     CDS             11781..13172
                     /gene="hydA"
                     /locus_tag="KPE55_001381"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0492472.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            13166..15727
                     /gene="xdh"
                     /locus_tag="KPE55_001382"
     CDS             13166..15727
                     /gene="xdh"
                     /locus_tag="KPE55_001382"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG0492473.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRSKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            15879..17225
                     /gene="rpoN"
                     /locus_tag="KPE55_001383"
     CDS             15879..17225
                     /gene="rpoN"
                     /locus_tag="KPE55_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG0492474.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            18023..19045
                     /locus_tag="KPE55_001384"
     CDS             18023..19045
                     /locus_tag="KPE55_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0492475.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            19105..20112
                     /gene="gap"
                     /locus_tag="KPE55_001385"
     CDS             19105..20112
                     /gene="gap"
                     /locus_tag="KPE55_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0492476.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            20334..21536
                     /locus_tag="KPE55_001386"
     CDS             20334..21536
                     /locus_tag="KPE55_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG0492477.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            21562..22305
                     /locus_tag="KPE55_001387"
     CDS             21562..22305
                     /locus_tag="KPE55_001387"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG0492478.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            22314..23846
                     /locus_tag="KPE55_001388"
     CDS             22314..23846
                     /locus_tag="KPE55_001388"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG0492479.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPAGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            24434..25726
                     /gene="eno"
                     /locus_tag="KPE55_001389"
     CDS             24434..25726
                     /gene="eno"
                     /locus_tag="KPE55_001389"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG0492480.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT        10514 a         2922 c         5191 g         7523 t
ORIGIN      
        1 gctaaactga taatttggta aattaaattc taatggggga aactatgtta agtaaattaa
       61 aagaatttca acaagaaatg ataaaataca ctgaaacagt agctagtgtg ttagatgtag
      121 atattgaaat agttgacgat agattaataa gaattagtgg tacaggtctg tataaatcaa
      181 agataaatga aagcgttgtt acagaaggat ttatttatga caatgttata cagacaggtc
      241 aggaattagt cgtattggat atatgtgata atcagctttg tatagaatgt tcccattaca
      301 tgaaatgcct aaataaagta ataatagcag ttccaataaa atataataat cgaactatag
      361 gagtaattgg tgctattagt acagataaaa ctaaaaaagt agaaataagt gcaaaaatag
      421 ataattattt aaaatttgtc aatcatatat gcgatttaat ttctatgaaa atagaagaac
      481 atgaagcgtc aaaaaatagc agtagaaaaa tggatatgat gatagaaatc attgaaaatg
      541 ttgaaaaagg cgttataata ctagacataa atagcaaaat atcttatata aacaatatag
      601 ctctaaaaaa attagatata gataaaaata taatagaaaa tatagtaaat atagtatctg
      661 ttgaatcttc ctcaaatgga catgaattac tagaaattga tattgataac aaaatttata
      721 atataaatgc caaaataatt ccggtatatc catatataaa tcaatacgac aaaataataa
      781 tatttgacaa gacatatatc aatcataaag gacatggaaa agtgaatagt ggctggggaa
      841 atagtgatat agagtctata attggaaatt cagaagcaat gttaaaagta aaagagagaa
      901 ctaagaaatt agcaaaatca aattcaactg tacttataac tggagaaagt ggtacgggta
      961 aagagctaat tgctagggct atacatgctg aaggtagcag atggaacaag ccatttattg
     1021 cgataaactg tgctgctatt ccagaaaatc tccttgaaag tgagctattt ggctatataa
     1081 aaggtgcatt tagtggagct agtagtggag gtaaagtagg taaatttgaa cttgcaaatg
     1141 aaggtgttat atttcttgat gaaataggag atttgtcaat gcctttacaa gcaaagttac
     1201 ttagagtttt acaagagaga aaatttgcga gaattgggtc aaataaactt atagatttag
     1261 atataagagt gattgctgct acaaataaaa acttattgaa attagtaaat gaaggtaagt
     1321 ttagggatga tttatactat agattgaatg taattcctat aaatttgcca cctcttaggg
     1381 aaagaaagga tgatattgag gcaataatga tgaagtttgc ttctaaatat tctttagaat
     1441 taggtattca gttaaataaa atagaagaaa atgtaatgaa tatgttgatt aactataatt
     1501 ggccgggaaa tataagggaa ctggaaaatg cagttgaata tatgatgaat ttggttggag
     1561 atgatggaat aatatataaa gatatgcttc cattagatat tttaaactat tataatatta
     1621 atggaaatat ttataaaaat aaggatgtaa atataatatt tgaagatgat atagttggag
     1681 ggatagtaga gaatcaagaa aggatacttt caattaaaga attagagttg acttacataa
     1741 acaagttgtt gaataagtat ggaagagata caaagacgaa gaagaagata gcaaaagact
     1801 taggcatagg tttggctaca ctatatagaa aattggaaga ggaacagtag taatataata
     1861 ttaaaatgat aggtatttgt gtgtttttat agataggtat ttttacttta tggtggaggt
     1921 gttattatgc aatatgcgat agagctatat tatgataaaa aaacggaaaa gcaacttttt
     1981 gatttatcta aaaaaatcgc tgatgaaaaa attagtacga agtacttaga atggaaaaca
     2041 agacctcatc ttactttggc ttgtttcaat gatgtggatg aggcttgttg catagacaag
     2101 ctaaaggggt ttgcacaaaa ccataaggta atacctgctt acattggttc agtaggcatg
     2161 tttaatgata ctaaaacggt gtttgtatca cctataatga atagcagtat gtatcagttt
     2221 cagagagaat tatatgaggt actacaagat tttgattcaa atggttggga atggtacacc
     2281 ccaatcgttg ggtaccccat tgcacaatag cacttactgg agatgatgaa agtgaggctt
     2341 tttttcgggc aagtgatgta ttactgcgtg agttcaaaaa aataagtggt gagtttgtct
     2401 caataggatt ggttaaaatt acatttcctg tggaagaaat ttttacagtg aatatgaata
     2461 ggtaacttta actctgttgt ggatgttatg aatattttgt atagtgcatt tcatcagata
     2521 aatgcgaagt tgcgaatttc gacagctcaa tagtcgcaag tagggcacaa tggtacacat
     2581 attgcacttt tgcttattgg ggggatgacc atgtaaactt ctgtaagaag ctatgaaaag
     2641 atttaagcca ttttggaggc tttgggtaat aaagaatata aagtatataa agtatataaa
     2701 tttatagagt ttatgcgtaa gaaggttata gccatatatt tatagaaaat aaaagtagta
     2761 atatatctga caaaacaata gacatataaa aaataagagc ataataaagg aagaattaag
     2821 acaaataaac gatttctcga attgataatt ctcgtattga taaaaatttc ccaatatgag
     2881 aattttttct tttataaatc ttatttttca cactcaaaat ttataaactt taaagaaatt
     2941 ttataaaagt atgtaattcc aacataaaaa aattgttttt agaataaata atattggcac
     3001 ggtaattgct ttgctataag gcacaaagat aaaaaattaa aatgacaccg aaaaatttga
     3061 aaggggaata taaaataaat gagagaaata aaatggaaat taaataactt acctaaatca
     3121 gaggaaaaag aaaaaggaat tgaattttta aaagatgaag agattgcaaa ggctaaagct
     3181 tttcatgaaa gctttccaca atatgaaaag actcctttag ttaaattaga taacttagca
     3241 aaatcactag gcgtaagcgg aatatatgtt aaagacgaat cttatagatt tggtttaaat
     3301 gcatttaaag ttttaggtgg ttcttactct atgggtagat acttagctca aagattaaat
     3361 acagatatat ctgagttagg gtatgataaa ttaacttcaa aagaaataaa agaaaaatta
     3421 ggagaaataa ctttctttac agccacagat ggaaatcatg gtagaggagt tgcttggaca
     3481 gctaataaat taggtcaaaa atctgttgta ttaatgccaa aaggttcttc tgaatttaga
     3541 ttaaataaaa tcaaaggaga aggtgcagat gctagtataa cagacttaaa ctatgatgat
     3601 gcagtaagat tagctaatga ttatgcagaa gctgatgacc atggagtaat ggttcaagat
     3661 acagcatggg acggatatga agaaatacca gcatggataa tgcaaggata tggaactatg
     3721 gctaaagaag ctatagaaca attaaaagaa tatggagtag atagaccaac acacgtattt
     3781 gttcaagcag gagttggttc acttgctggt gcagttcaag ggtatgttgc ttcaatatat
     3841 gatgaatgcc caataacagt agtagtagag gctgatgagg ctgattgtta ctataaatca
     3901 gcaaaagctg gagatggaaa accaagattt gttggaggag atatgccaac tataatggca
     3961 gggcttgctt gtggagaacc aaacactata ggttttgaag tgttaaaaaa ttattcatca
     4021 gcattcgttt cagcaccaga ctgggtatct gctaaaggta tgagaatatt aggaaaccct
     4081 ctaagaggag atgagaaagt aatctctgga gaatctggag cagttacaac tggagcatta
     4141 gtttcaatcc ttgaaagtga agatttaaaa gacttaagag aagctttgaa actagatgag
     4201 aactctaaag tattattaat aagtacagaa ggagatactg accctgataa atatagagat
     4261 atagtttgga atggtgaatg ccaaagtaaa taaaagactt aaggaaaaga aaaacttaat
     4321 aaataaatga aaaagtaaag caaataaaaa agtaaagcaa atgaaaaatt caaaataaaa
     4381 actaaataaa attaaataaa accaaataaa attaaaataa agtctaaata aaaatttaat
     4441 aaataaaata taaagaaata aaaaatcaat agattaagtg aaatgctaat aaattaaaaa
     4501 agtgaaatat caaaataaaa cgtcaacaaa ttaaaaatta tatgggggaa tatcaaaatg
     4561 ttaaatgaaa caagaaaaaa acaattaact gaagtatgcc aagacttaat aagaaatgct
     4621 agttattcag gtcaagaaga aaacgtagta aaagctatag aagaaaattt tgctttacta
     4681 ggttttgata gttggtcaag agatagatat ggaaatatag taggatgtat aaaaggaaat
     4741 aaaccaggta aaaaaatatt atttgacgga catatagata cagttccagt tccagatgca
     4801 agtaaatgga gtgttcctcc ttttgaagcg agaatagtag atgggaaaat atatggtaga
     4861 ggaacttctg atatgaaggg gcaagtaagt gctatgatgg ctgctgtttc atactttgca
     4921 gaagatacta acaaagactt tgaaggtgaa ttgtatgtag caggtgttgt tcatgaagaa
     4981 atatttgaag gtgtttcagc tagagagata agtaaggctg ttcaacctga ttatgttgta
     5041 ataggtgaat cttcagaatt aaacctaaag ataggtcaaa gaggtagagg agaaatagta
     5101 gtagaaactt ttggtaaacc agctcactct gctaatccag aaaaaggtat aaatgcagtt
     5161 tataaaatgg ctaatgtaat ccaaaagata caacaattag tacctccaac tcatccagtt
     5221 ttaggtgatg gaatattagt tttaacagat ataaaatctt ctccatatcc aggagcatca
     5281 gtagtgccag attattgcaa agctactttt gatagaagat tattagttgg agaaacaaga
     5341 gaaggcgtat tagctccaat acaagcttta ttagatgaaa tgatgaaaga agacccagaa
     5401 ttaaatgcaa aagtttctta tgcagttgaa aaagctgatt gctatacagg aaatactata
     5461 gaaagtgaaa gattcttccc aggttggtta tatgatgaag aagatgaatt tgttcaagca
     5521 gcgtataagg gattaaaaga agctggaata gattcagaaa taactcaata ctcattctgt
     5581 acgaatggaa gtcactacgc aggtgaggct ggaataagaa ctataggatt tggtccttct
     5641 aaggaaaatc ttgctcatac aatagatgag tatatagaat tagaccaatt attcaaaggt
     5701 acagaaggat attatggtat attaaaatca gtttataaaa aataattagg agatggtttt
     5761 gtgagaactt taataaaaca tggacttata gtagatggaa ataaaacacc agcatatgaa
     5821 ggagatattt taatagaaaa cgagaaaata ttaaaaatat cacaagattt aaacgaagaa
     5881 gctgacaaag ttatagatgc aaaaggtaga gttatatgtc caggtttcat agatactcat
     5941 agccattcag acctagttat tttagttaat ccatataatg aggtaaagat tagacaggga
     6001 attacaacag aagtgttggg ccaagatggt atatctatgg cccctctacc tcaagagcat
     6061 atatcttctt ggagaaaaaa tttagcaggt ttagatggtg aaagtgatga aattgattgg
     6121 aaatatgaaa caacagagaa ttatttaaaa atgatggatt acaatggggt aggtttaaat
     6181 gaaacttatt tagtacctca tggaaacgtt agaatggaag ctatgggact tgaagataga
     6241 cctgctacta aagaagaaat tcaaaaaatg tgtgaaatca cagagagaga attaaaagca
     6301 ggagctatag gattgtctac tggacttata tatatacctt gtgcatactc attgactgaa
     6361 gaaattatag agatgtgtaa ggtagtagca aaatatgacg gagtatttgt agttcaccaa
     6421 agaagtgaag cagatacaat tttaacttca atggaagaaa tcatagaaat tggaaaacaa
     6481 tcaggagtaa aagttcattt ttcacatttt aaggtttgtg gtaaagcaaa ctggaagtat
     6541 ataccacaag ttatagagct tttagaaaaa gcagaaaagg aaggaataag agtatctttt
     6601 gaccaatatc catatgcagc aggaagtaca atgcttggag tagttttacc tccttgggca
     6661 cattctggag gaactgataa gttaatagaa agattaagtg atgaaaacga aagagcaaaa
     6721 atgaaaaaag atatagcaaa tggaatagaa ggctgggata attttataga atttgctgga
     6781 atagaccaaa tatttgtaac aagcgtaaaa acagaaaaga ataaagatac aattggtaag
     6841 agcttattag aaattggaaa aatgagagga aaagacccat tagatgcaac gtttgattta
     6901 ttaaaagaag aagaaaatgc agttgggatg gtagatttct atggattaga agaacatata
     6961 ataggattca tgaaaagaga cgagcaaaat gtgtgtacag atggtctttt agcaggtaaa
     7021 ccacatccaa gagcttatgg ttctttccct aaaatactag gaagatatgt tagagagttg
     7081 aatgtgttaa caatcgaaga agctgtttat aaaatgacta aaaaggcagc tagaagcttt
     7141 agtataaaag atagaggcga attaaaagaa ggatattttg cagatatagt tatatttgac
     7201 aaggatacag ttagtggttg tgatgatttt atcaactcta tgcagtaccc tacaggtata
     7261 gattacgtaa ttataaatgg aaattgtgtt gttgaggaag gaaaatacaa taaaattaaa
     7321 gcaggaaaag tacttaaaaa ttaaatctta gctttaagga ggaataaatt tatgatttta
     7381 gtggggaatg ggaggctaat aagccaagat tctttaaatc cctatatgga agatggatgt
     7441 gtagtaataa atgataatct aattgaagat ataggtacta cagaagaaat gaaagttaaa
     7501 tatccagtac atgaatttat agatgcaggc aagaaaataa tcatgccagg tctaataaat
     7561 tcacatatgc atatatacag ctcatttgct agaggaatgg cagttccagg aaaaccttct
     7621 gagaactttt tagagatact aaacaattta tggtggagac ttgacaaaca gcttacactg
     7681 gaagatacaa aatacagtgc atatgcaacg tatatagaat gtataaaaaa tggtgtaaca
     7741 acagtttatg accatcatgc aagtccaaat gcaattgaag gcagtttgtt tacaatatca
     7801 gatgttgcaa aggatttggg aattagaacg tgtctttgtt atgaagtgtc tgatagagat
     7861 ggtggaaata caatccaaca aggtatcaat gaaaacgtaa actttataaa atacgctcaa
     7921 aaagatgata cagacatgat aaaaggaatg tttgggcttc atgctgcatt tacactatct
     7981 gatgatacat taaataaatg tgcttcagag atggctggtc ttgatacagg ttaccatgta
     8041 catgttgctg aaggactagc agacttacaa tataatgtag ataaatatgg aaaaagggtt
     8101 ttagaaagat tcaatgattt tggagtacta aaagaaaaaa caatagcagt tcattgtata
     8161 tatataagtg cagaagaaat cagtctttta aataaaacaa atacatttgt agttcataat
     8221 ccagaatcta atatgggaaa tgcagttgga tgttctcctg caatagagtt attaaaaagg
     8281 ggagttacag taggattagg aactgatgga tatactagcg atatgtttga atcattaaaa
     8341 gttgccaata taattcacaa acatcactta aaagacccaa gagttggatt tgaagaatca
     8401 caaacaatgt tatttgataa caatagaaaa atagctaaag cttattataa aaatgaccta
     8461 ggagtactta aaaaaggtgc ttatgcagat gttatagtag ttgattatat accacatact
     8521 ccattgacag aaaatacaat agggggacac actatttttg gaatgagtgg aagaagcgta
     8581 gatacaacta ttataaatgg aaaagtactt atgaaagata gaaagttaat aaacatagat
     8641 gaagaaagta ttctagcaaa aagtagagaa ttaagtcaaa atctatggaa tagaatttaa
     8701 ataattgata ttaatataca agtaagaaac aagaatttag atgttaaaaa cagagcttaa
     8761 acaaaaatag gaatttaaat gaagtatata aataaaaaat atacggaata gaatattaaa
     8821 ttaaatatag aagggagacc caaaatgtct cgagataaaa gccctaaaat agctccagta
     8881 gatgaaaggt tacctgtatc caaatcttgg gtattttcac tacagcacgt tttagcaatg
     8941 tgtgcaggag cagtagcagt tcctatgatg ataggaagtg ctgcacgact agatcatgct
     9001 agtattgttt ttttaataaa tgcttgcttg tttatggcag gtataggaac actcatacaa
     9061 agtgttggta taggaaatat tgtaggtgct aaaataccag taatagaagg aactagtttt
     9121 gccgctgtgg caggtatgac agcaatagcc tcatcatata gtgattcaca agttgcaata
     9181 cagacagtat ttggctctgt aatagtagct ggattgtttt gtatattact tgctccagta
     9241 tttgaaaaat taataagatt tttccctagt gtagttactg gaacagttgt tatggtaata
     9301 ggtatttcac tatttccagt tgggataaaa tggataactg acaacaaatt agaagcggtt
     9361 gaaccaaact cattagcatt agctttagca gtattggcaa taactttact ttcatttaaa
     9421 ttcttaaaag gaatttggaa tagcacagct atattggtag gtatagtact tggtacacta
     9481 ctttccatgg cagttggaat ggcagatttt agtgcagtaa atggagttgg ttggcttaac
     9541 ttaaatgtac ctttaaaatt tggaatgcct agatttgaac catctgcaat actatcattg
     9601 tgtttgatta tgttggtact tatgacggaa tcagttggta atatgatagc tattcatgaa
     9661 atggtagaca aagaagtaac aggtaaaaat attaaaagag gtctatttgg agatggaata
     9721 tctacagcat tatctggaat attcaattca tttccaataa ctccatttgg tcaaaataca
     9781 ggaatagtag gtcttacagg tataaaatca agatttatag cagtttacgc aggggttata
     9841 ttgctagtac ttggattctt accaaaattt gcagcaataa taggagcaat acctaagcct
     9901 gttcttggag gcgtaggatt cgcaatgttt ggaatggtaa tggttggagg agtaaaaact
     9961 ttaagcaaag taaaatttga tggaactaaa aatggggtta tagtagcagt tagtgttggt
    10021 ttgtctatga taccactaac aaatcctgat ttctatcata atttcccaac ttgggtacaa
    10081 acaatattcc atagtggaat aacaacaggt agtttatcag cagtgctttt aaacatattc
    10141 ttcaatgaac taggttctaa aaaagaaaaa aataataaag ttgaagaagc aaaataaaaa
    10201 taaaacaagt aaaaatctat attgacttaa agtcattaag tattaataaa aataattcag
    10261 agatattaat aattcaacaa aactatctta tattttaaat aatataaaaa agtaaataaa
    10321 taacgaatac aaataaaaga atacaatcta aatattaaaa atatttaatt taagggagag
    10381 ataataatat ggctaattta aaagttaatt tatatggaat ggaattttta aacccaatta
    10441 tgacagcggc aggacctgga gcaaaagatg gtgctctctg tcaagaggca gcaaaaggtg
    10501 gagcaggagc tatatgtaca aaaactatat ctgtattacc agctgatgta ccaagaccat
    10561 gtatggcaaa aactaatagt ggttttttaa acacagaatt atggtcagag cttccaaaag
    10621 agcaatggat agaggaagaa tacaaaaagg taaaagaaac agggcttcca gtaatagtaa
    10681 gtatgggata ttcagcagag caaatagctg aggttgctcc tttagtaaag ccttatgcag
    10741 atgcagttga attatctact cattatgtag gtacagacgt tgcaccaata gtaaatgctt
    10801 taaaagctgc aaaagctgtt ttagatgtac cagtatttat gaaaatgagt cctcatactg
    10861 acattcaaac tattgcaaaa gctgttgaag aagcaggtgc agatggactt gtaatgataa
    10921 actcatttgg accatgtatg gcaatagatg taaatacagg ataccctata atgggaagta
    10981 aagctggata cggatggtta tcaggtgcac caataagacc tctagcaata agatgtatat
    11041 atgaagcttc tcaagttgta aatataccaa taatcggtgt tggtggaatc acttgtggaa
    11101 aagacgtggc tgaaatgttg atggctggag caagtgctgt tcaagtatgt acagaagcaa
    11161 tccttaaagg gccttctatc tatggaaaaa ttgctaaaga attaaatgaa tttttagatg
    11221 agaatggata tgctgatgtt aatgaaataa aaggattaac tcataagaaa atggcagcta
    11281 gagggtttag aactcattca gtagctccag tagtggatga taacaagtgt atcaaatgta
    11341 agcaatgtgt aacaagctgt gtatacgaag ctttagaagt tacagataag ttgaacatag
    11401 atgcagacaa gtgttttgga tgtggattat gtgtaactag atgtccaaaa ggagctttaa
    11461 gcatctgcta caatcaagta gaagctatga atgagtaata attatataga attttggtaa
    11521 ttaacttaga gtaataggta ttaggataat aaaatatatc agataaacaa acacatatta
    11581 tgcattaata atacagtcta aagttaataa ttttattata ttgatttaac taaatattag
    11641 tttagctgaa tattaattta attaggtatt ggtttaatct taatatagat ttactacgta
    11701 taagtgaact aactagatta ctttaagtta attttttcat ataaaataaa ttgatacatt
    11761 acaagtaagg tggagtctat atgggaattg ttttaaaagg tgggaaagta gtaacaagtg
    11821 aaaaaactta ctgtgcagat gtaagaatag aagatgaaaa aatagtcgat ataggtcttg
    11881 atatatcaaa agaaaatgat gaaatagttg atgttagtga ttcttatgtt ataccaggag
    11941 gtatagatac acacacacac tttgacttag atacaggcgt tactaagaca gcagataact
    12001 ttgagacagg aacaaaagct gctatagtag gaggaactac tactatattg gattttgcta
    12061 cacaagctaa aggatgttct ttgaaagacg gattagaaga atggcatgaa aaagctaagg
    12121 ataaatccta ttcagattat ggctttcata tggctataac agattggaat gactcggttt
    12181 gtaatgaaat ggaggatatg gtgaaagagg gagtatcttc atttaaactg tatatggcgt
    12241 ataaaggaag ccttcaagtt gatgatggtg taatctttga agcattgaga aaagcagaag
    12301 agataggagg aattattggt ttccattgtg aaaatgggga cataatatgt gaacttgtgg
    12361 ataaagcaaa gtcagaaaat aatttatctc caaaatatca tcaattaact agaccagcta
    12421 taatggaggc agaagctaca tcaagactta tgaagatagc tgaagttgca aattctaaag
    12481 tatatgtagt tcacttaagc tgtaaagaat ccttagagga ggttttaaaa gctaagcaaa
    12541 gaggtgtgga tgtaatagtt gaaacttgtc ctcaatacct tctattaaat gatgaattat
    12601 atgatttaga tggatttgaa agtgctaagt atgtaatgtc accaccttta agaaataaag
    12661 aaaataatga aatattatgg aatgctattt tagataatca aatagacact ataggaacag
    12721 accattgttc ttttaattat aagggacaga aggaacttgg gataaacgat ttcagcaaga
    12781 taccaaatgg ttctccaggt gtagaacata ggatgagtct tttatacaca tatggagtct
    12841 gtaaaaataa aatatctatc aacaagatgg tagaagtaac atcaactaat ccagcaaagt
    12901 tgtttggaat gtatcctaat aaaggaacta tagaaatagg cagtgatgca gatatattag
    12961 tcttaaatcc aaacaaaaaa tccaaaatag aagcaaaaaa acaattacaa aatgtagatt
    13021 atacacctta tgaagggtat gaagtggatt gtcaaataga acatgtttat ttaagaggaa
    13081 aagaagtagt tagagatgga gaattaattt gtaatactcc aagtgggaga tatataaata
    13141 ggcgtctaac aaagggggct agagaatggt agaatttata ttaaatggag agaaagttgt
    13201 tgcaaaagaa ggcaaaaaac ttttagactt ccttagagaa gatatgaata taacttcagt
    13261 taaaaatgga tgttcagaag gtgcttgtgg aacatgtatg gttataatag atggtaaagc
    13321 tactaaagca tgtgtattta aaactgataa attagctggg aaaaacataa caactataga
    13381 gggattatca gagagagaaa gagatgtttt tgcatattct tttgctagtc aaggagctgt
    13441 acaatgtgga ttttgtattc caggtatggt aattagttca aaagcactta tagacgttaa
    13501 taataatcct actgaagatg atataaagaa agctttaaaa ggcaatatat gtagatgtac
    13561 agggtatgta aaaataatta aagctgtcga tttagcggca aagattttta gagaaaatat
    13621 agatgtgcca aaagtaaact gtaagggttt agtaggtgaa aatcttccta gagttgatgc
    13681 agttggcaaa gcacttggat attctgaata tgtagatgat atgagaattg agggtatgat
    13741 ttatggttca gcagttagat ctaagtatcc tagagcttta gttaaaaaaa tagatataga
    13801 agaagcaaaa gcttatccag gagttgttgc agttctaaca gctaaagata ttccaggaac
    13861 tataaaggta ggacacttaa agaaggattg ggatacttta ataccagaag gtgaaataac
    13921 tagatactta ggagatggaa tagttttagt agcagcagaa actagagaag cgttagaaga
    13981 ggccaaaaag ctagttaaga tagattatga ggaattaaca ccattaagta ctacagcaga
    14041 tgcacttaga gaagatgctc caaagataca tgagacaggg aatatacttg ttaaggaaca
    14101 tttagtaaga ggaaacgcta aagaaaagat agaaaattca aaatatgttg taactaaaag
    14161 atatactaca cctgctacag aacacgcatt tttagagcct gaatgtgcag tagctggtcc
    14221 ttatgaagat gatggagtaa taatctattc aacagaccaa ggggtgtttg ccactcaaca
    14281 tgaatgtgca gatatgttag gattaccata tgagaaggta agagttataa acaaaatggt
    14341 aggaggaggg tttggtggaa aagaagacat gagtgttcaa caccatgcag ccttacttgc
    14401 ataccataca aagagatttg taaaagtaca gttaacaaga aaagaaagta ttattgttca
    14461 tccaaaacga catgccacag aaatggaatt tacgacagca tgtgatgaaa atggatactt
    14521 aacgggtatg aaggcaacga taatatctga cacaggtgca tatgcctctc ttggaggacc
    14581 agtgctacaa agactgtgta ctcatgcagc aggaccatac aattatcaag atattgatat
    14641 aacaggaaca gcagtatata ctaataaccc tcctggagga gcatttagag gatttggagt
    14701 tacacagagt tgttttgcaa ctgaatgtaa tctaaattta cttgctgaaa tggttggaat
    14761 atctccttgg gagataagat ataaaaatgc aataagacct gggcaagttt taccaaatgg
    14821 tcaatttgca gatgaaggaa cagcaatggc agagacacta gaagctgtaa aagaagagta
    14881 tgaaaagcat ccacgtgcag gtatagcttg tgctatgaaa aatagtgggg ttggagttgg
    14941 tatacctgat gttggaagat gtagattggt gataaaagat ggcaaagttc acataagaac
    15001 aagtgcttct tgtataggtc aagggcttgg tactgttatg actcaagtag tttgtgaaac
    15061 tgctaaccta cttcctgaaa atatagttca tgaagttcca gatactcatt taacaccaga
    15121 ctctggtaat actacggctt caagacagac agtatttacg ggagaagcta ctaaggttgc
    15181 atcaaataag ttaaaagaag ctttagactc tggcaaaact ttacttgatt tagagggaga
    15241 agagttttat ggagaatatg aaagtattac agataaaatg ggttgtgaca aagaaaatcc
    15301 agtaagtcat gttgcttatg gatatgcaac acaagttgta attctggatg aaaatggaaa
    15361 actagaaaaa gtagtagcag cacatgatgt tggaagagct ataaatccaa tcagtttaga
    15421 aggtcaaata gaaggtggag tagttatggg tcttggatat gctctaaccg aagattaccc
    15481 agtagtcaac tctgttccta cagctaagtt tggtacatta ggtttattta aatctacaga
    15541 tataccagaa atacaagctt taatagttga aaagaacaaa gatttattgg cttatggggc
    15601 aaaaggtgta ggagaaattt gtacagttcc tactgcacca gcagtacaaa atgcctatta
    15661 tgtatttgat aaagaattta gaactgagtt accactacaa aatactccat atagtaaaaa
    15721 aaagtaacaa aaaaaagaaa tattaaataa aaaaatagat gaaattgtaa ataaaaaaaa
    15781 cccttttgca taaggtatat tttaaatgat agaatataca tatacaggag ggtttttctt
    15841 ttataaaata agtttaataa aattgagagg tgagcagaat gaattttaat aatagtctag
    15901 aattaactca gtctcagaag ctaataatga ctacacaact taagcaatca ttatcaatat
    15961 taaatatgag taagttagag ttagaagaag agataaaaag agaagcagag aataacccat
    16021 tagttgaagt cgagaaaagt ggggagataa attgggaaga gtatataaaa gacatggata
    16081 ggtcaagacg tttagataga acagagataa gctataatcc tgataatgaa gttaatctag
    16141 aaaatttagt taaatattcg tcaaatttat atgaagattt aaaatttcag ataagcctgt
    16201 ataaacttac agacaaagaa ttggaagtct gtgagtatat aattgatagt ttagatgagg
    16261 atggatattt aagaactgag gaaaaagtaa ttgttgatac cttaaagata gatgaagaac
    16321 tttttgaaaa atgtttaatt agtgttcagc agttggaacc tagtggagta ggggcaagaa
    16381 gtctttcaga atgtctgatg atacaaatgg ctagtttagg aatatataat gaaattttag
    16441 aggaaatcgt tacaaaagac ttaaatttaa ttgcaaataa taaatataaa gagataagta
    16501 aaaagtacaa tatgtcatta caaaaatgtg ttgaactaat aaacataatt aagacattag
    16561 accccaaacc aggaagaact tgttcagtag agaaaagtgt atacattcaa cctgatgtaa
    16621 cagtagaaaa aattgatgat gaatttattg tatatttaaa tgagaaagat tcttatcaaa
    16681 taagaatcaa tagttattat aaagacatat taaaaaattc acagtctgat gaaagtgcaa
    16741 aagaatttat aaaagaaaga ctaaattcag caacaggact tatgaaaaat atagagagta
    16801 ggaaaactac agtacttaag attgcagaag agatagtaaa agctcaagat gaatttttga
    16861 gaaaagggac aaaatatata aaacctttga aaatgaaaga catagcagaa aaattagaat
    16921 ttcatgaatc tactataagt agaggtgtaa atggtaaata tatgctaaca ccttttggcg
    16981 tatatgaatt taggtacttt ttcagtagcg ccattgagac tgagaataat gaaatggcat
    17041 ctagtacaag cataaaaaaa attataaagg aaacaataaa agacgaaaat aaaaagaaac
    17101 ctttaagtga tgaccatata tctaagttac taaaagaaaa aggaatcaat gttgcaagaa
    17161 ggacagttgc caaatatcgt gaagaattag gaatcccatc ttcaagtaag agaaaagaat
    17221 actaataagt aaaagacaaa tatcagtatg gtaaggggaa aaggctaatt aagagatgaa
    17281 tgtagattgg gctaaaaatt ttataaagta aaataatata atatgaaaaa gtattaaaat
    17341 gctataaggt attaaaaaac tggggaacat taaaacatta agatagcaaa gaacataaaa
    17401 aactaaagaa gattaaaaca ttaaagaaca ttagagcttt gaggaatatt agaactttaa
    17461 agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac tggggaacat
    17521 taaaatattg taaaatgcta aaaaactaga aaacataatt aaataagtgc ttataaagaa
    17581 tgattaaatt aaataagtgt ttgtaaagaa taattagata ttagaaaaag aagaatacct
    17641 agataaataa actattatta aatttaataa tcacaaaagg aaaacacgta gggtataaat
    17701 aaaaatctta actagataaa agtaaactta gttgtagata aaaataacta gaaataaaag
    17761 taactagaaa taaaattatt tatagtaata aatattagaa tgaaaatata aaaaaatata
    17821 tataaaaagc cttatataaa aagaaaaaat actgtgattt gaaaatggtg gagaaatatt
    17881 ttataaaata aaaaaataaa aaagtttttg aaaaactatt gaaatctata cgcaaatcct
    17941 ataaaataaa aatatcttgt gggacttaaa taaacactag ggacaaaaaa agtcccgaaa
    18001 ggaaagcaat aaggagtatg ttatgaagaa cttattagaa attcaaaaga aattgattcc
    18061 acaggcaatt gaacttatgg aaagaagata ctctatttta agacaaatat ctttaagtga
    18121 accaataggt aggagaacat tatcaaatgt attagagatt agtgaaagaa taatcaggtc
    18181 agaaactgag tttttaaaag aacaaggact tatagacgta gctgtatctg ggatgactat
    18241 cactaaagaa ggttctgatt tgttagataa acttaaggac atcatgagtg acatcatggg
    18301 attgtctaag ttacaagata gagtcagaga aaagcttgga ataaaaaggg taatattagt
    18361 tcctggaagc tgtgatgaaa atggaagttt actaaaagat gtaactagat atggatgcga
    18421 atatttctta ggcattttaa aagatggaga tatcgtttca ataacaggag gaagcactat
    18481 gttggagttt tcaaacgtta ttaaaactga taagagatat ccaaactcta caatagttcc
    18541 agctagagga agtatgggaa ctgatgtaga gatgcaatct aacaatatag ttgcttcaat
    18601 aagtaagaaa ttacattcta attgcaaact tttacatata ccagatgagt tggatgaaag
    18661 tgcaatgaaa actcttagcc aagtgccaga gattaagaca actttggagt atattaaaag
    18721 aacagacatt ttagttttta gtataggtag agcagatgtc atggcaaaga gaagaaaact
    18781 tccagaagac aaggttgatg agatattatc taaaaatgca gttggagaag catttggata
    18841 ttatttcaac aaagatggag aaatagttta caaactgaac actgttggaa ttgatttaga
    18901 aacagttaaa caagtaaaag aaactattgc aatctttgca gggactgaaa aagtagaagc
    18961 cttattagct atatcaaata tgcataaaaa catggttctt gtaacagatg aagaaagtgc
    19021 ttataagata ttgtcattat tataaaagtc aagacctagc acagagctag aaaacataca
    19081 tttactttat aggaggtagc gaaaatggtt aaagtagcta taaatggatt tggaagaata
    19141 ggaagattag cattaagaaa aatgatggag caacaagata agttcgaagt tgttgcaata
    19201 aacgacttaa cagacgctaa aatgttagct catttattta aatatgatac agctcaagga
    19261 agatttaacg gagaaataga agttaaagaa ggtgcttttg tagtaaacgg aaaagaaata
    19321 aaagtaacag cagaaagaaa tcctgctgac ctaccatggg ctgaattagg agtagatata
    19381 gtattagaat gtacaggatt ctttacatct aaagataaag ctgaagctca tatacaagca
    19441 ggggctaaga aagttgttat atctgcacca gctactggag atttaaaaac aatagtattc
    19501 aacacaaaca gtgacatatt agatggttct gaaacagtta tatcaggagc ttcttgtact
    19561 actaactgtt tagctccaat ggctaaagta ttaaatgata aatacggtat agaaaaagga
    19621 ttaatgacta ctatacatgc atacactaat gaccaaaata ctttagatgg tcctcatcca
    19681 aaaggtgatt taagaagagc tagagctgct gctggaaaca tagttccaaa cactacagga
    19741 gctgcaaaag caataggctt agtaatacct tcattaaaag gaaaattaga tggggcagca
    19801 caaagagtac cagttgtaac tggttcaata actgaattag tttgtacatt aggaaaaaat
    19861 gttacagttg aagaaataaa tgcagctatg aaagaagctt caaacgaatc tttcggatat
    19921 actgaagaaa tgttagtatc ttctgacata atcggaataa gctacggttc attatttgac
    19981 gctactcaaa caaaagttat ggaagtagat ggaaaacaat tagttaaagt tgtttcttgg
    20041 tatgataatg aaatgtctta tacttctcaa ttaataagaa ctttaggata ctttgcacaa
    20101 ttagcaaaat agtcagtttt gagaaaatag aaaagtagat tgacaaagtc cgagtttgta
    20161 gtttaatact acggactcag gacttttttt aagaaaaaat atataaaata cacctaaata
    20221 atgttacata ctatatataa aaatatgata atatgatata tttaataggg taaaagtatg
    20281 tactatatta tacttggcaa taaaaaatta ctttattaaa aggagagata tttatgtcaa
    20341 tgcttaacaa aaaaactata gaagatattg atgtgtgcgg aaaaaaagta ttagtaagat
    20401 gtgactttaa tgtgccatta caagatggtg taattactga tgaaaataga ttgaacggag
    20461 ctttaccaac tattcaatat ttaatttcta aaggggcaaa agttatatta tgctctcact
    20521 taggaaagcc aaaaggagaa gctaaaccag aattatcttt agcaccagta gcaaagagat
    20581 tatctgagat gttaggaaaa gaagttgtat ttgcagcaga cgataatgtt gtaggagaaa
    20641 atgcaaagaa agcaactgaa aaaatggaaa acggagatgt agtattatta gaaaatacta
    20701 gatatagaaa agaagaaact aaaaacgaag agaatttctc taaagaacta gcatcacttg
    20761 ctgaaatatt tgtaaatgat gcatttggaa ctgctcatag agcacattgc tcaacagttg
    20821 gtgcaggcga gtttttacaa gagagagttt gtggatactt aattcaaaaa gaattaaaat
    20881 tcttaggaga agctgttgca aaccctgtaa gaccatttac agctatactt ggaggagcta
    20941 aagtttctga taaattagct gttataaacg aattattaga aaaagttgat aatctaataa
    21001 taggtggagg aatggcttat acattcttaa aagctcaggg ttatgaagta ggaacttctt
    21061 tacttgaaat agataaagtt gaatatgcta aagaaatgat ggaaaaagct aagaataagg
    21121 gtgttaactt attattacca gtagacgttg ttatggcaga ccactttgca ccagatgcta
    21181 ctccaatagt aacagaagat gctaatgtaa aagaagatta catgggacta gatatgggac
    21241 ctaagactat agcaaacttt gtaaaaacta taaaagaatc aaaaactgta gtatggaatg
    21301 ggccaatggg agtatttgaa tttgaaaact ttgcaaatgg tacattatca gtagctagag
    21361 ctatggcaga gttgactgat gcaactactg taattggcgg tggagatagt gcagcagcag
    21421 ttaatcaatt aggatttggt gacaagatga ctcacgtatc aacaggtgga ggagcatcac
    21481 tagaattttt agaaggtaaa gaattaccag gtattgcagc attagataat aaataaacta
    21541 atatatagag gagattgtaa aatgagaaaa cctataattg caggaaactg gaaaatgcat
    21601 aaaacaataa aagaagcttt agaatttgta aatgaagtta aagataaagt aaattctgat
    21661 aaagtagaag ctgttatatg tgcaccattt actttattaa aggatttaaa agaagctact
    21721 aagggtacaa atataaagat aggtgctcaa aatatgcact ttgaagaaaa aggagcattt
    21781 acaggagaag tttcacctct tatgttaaaa gaaatagata tggactatgt tgtaatagga
    21841 cattcagaaa gaagacaata cttcaatgaa actgatgaaa ctgtaaacaa gaaagtttta
    21901 aaagcattag aagtaggaat agacccaata ttatgtgttg gagaaacttt agaacaaaga
    21961 gaagctggaa aaactaaaga tgtttgtaaa gttcaagttg aaaaagcttt agaaaatgtt
    22021 cttaaagatg atttagcaaa agtagttgtt gcatatgagc caatctgggc tattggaact
    22081 ggtaaaactg caactgctga agatgctaat gacgttatat cttatataag agaagttata
    22141 aaaggattat atggagaatt agctaatgaa gttagaatac aatacggtgg aagtgttaaa
    22201 ccttcaaatg tagccgaaat aatgggtcaa agtgatatag atggagcttt agtaggtgga
    22261 gctagtttag ctagtaatga ttacctagac cttgttaatt tctaaggagt gaaatgatga
    22321 aaaaaccagt tgctttaatt attatggacg ggtttggata caataaggat gttaagggaa
    22381 atgcaattgc tgagtccaaa actcctaatt tagatagaat aaaaaaagag tatccaaaca
    22441 cattgataaa tgctagtggt cttgatgttg gtctacctga tggtcaaatg ggaaattcag
    22501 aagttggaca cactaacata ggtgcaggta gaatagtata tcaagattta actagaataa
    22561 caaaatctat aaaagatggt gactttttta ctaataaagt tttatgtgaa gcaatggata
    22621 atgcaaaaga aaattctctt catgtaatgg gacttttgtc tgatggagga gtacattcac
    22681 atatagacca tcttaaggct ataatcaaaa tggcaaaaga taaaggcgta caaaaggtgt
    22741 atgttcacgc atttactgat ggtagagata ctgaccctca aagtgcatta gaatatgcca
    22801 aagaagtaca agctagtatg gatgaaattg gagtaggtga atttgctaca gtttctggta
    22861 gatactacgc tatggataga gataagagat gggaaagagt agaacttgca tacaatgcca
    22921 tggttagagg tattggtgaa aaagcaaatt ctattgagga agctattcaa aactcttatg
    22981 atgatggaaa aaatgatgag tttataatgc caacagttat tatgaaagat gataaaccag
    23041 ctggaagtat aaaggaaaat gattcaatta tattcttcaa ctttagacca gatagagcta
    23101 gacaaataac tagagcttta gtttgtgaag agtttgatgg atttaaaaga gaagatatta
    23161 aaaacttctt tgtatgctta acagagtacg atataactat agagaatgtg catatagcat
    23221 ttggacctca atctttagcc aacactctag gtgagtatct agctaaaaat ggaaaaactc
    23281 aacttagagc tgctgaaact gaaaagtatg cacatgtaac tttcttcttc aatggtggag
    23341 tagaagagcc aaataaaggt gaagaaagac tattgatacc ttctccaaag gttgcaactt
    23401 atgatttaaa accagaaatg tcagcatatg aattgacaga taaagcttta gataaattag
    23461 gagaagataa gtttgacttt atagtattaa actttgcaaa tcctgatatg gtaggtcata
    23521 caggaagtat agaagcggct ataaaggctg ttgaaactgt agatacttgt gtaggaaaat
    23581 tgatagataa aatagtagag ttgggtggaa gtgcaataat aactgctgac cacggaaatg
    23641 ctgaatatat gttagaccca gaaacaggaa aaactgtaac ggctcactct attaatccag
    23701 taccatttat tgtagttggt caagaatatg aaagtgcaaa attattggat ggaggaagat
    23761 tatctgatat agcaccgact gttttagata tgatgaagtt agaaaagcca gaagaaatga
    23821 ctggtcattc acttatttca aaatagtcga ttttgataag tagagaataa tatataaact
    23881 acaatataaa tttaaaaata atgcagttta ggtttatttt gtaaagttta gactttatga
    23941 cataagattt aaattttgta agttctggat attgtaaggt gtagatgaag atatatttta
    24001 taaaatttac aagatagaca atttataaaa taaaaaatta caatatagat aataatgtaa
    24061 ataatgatat aaataataac aagatagaca ataacaatat aaataataat aattaatata
    24121 tattatataa gcaaaaattt tagaagcatt agttgtacat tttatgttta atgttttaaa
    24181 tctagttata atatttgtgt atttaagatt ggttttggat tttagacaag tttttttata
    24241 ttatataaat taaaatatta taaaaaataa aataaaaata ggaactttta caaatttacc
    24301 catcaaataa atttgtacct aattttcaaa agcaacttta tgtactataa tatagaaagt
    24361 agattaaaat agtagacgga tgtttacagt aaatgaaaat ttaactaata gaaaatcgaa
    24421 gggagatata caaatgtcag ttattgaatt agtatatgct agagaagtat tagactcaag
    24481 aggaaatcca actgtagaag ttgaagtagt attagaagat ggagcaatgg gaagagcaat
    24541 agttccatca ggagcatcta caggagcttt tgaagctgtt gaattaagag atggagataa
    24601 gggaagatac ttaggaaaag gtgtagaaac agctgttgct aacgtaaatg aaataatagc
    24661 acctgaaata gaaggaatgg atgcatttga ccaaccagct atagatgcaa tcatgataga
    24721 attagatgga actccaaaca aaggtaaatt aggagcgaat gctatattag gtgtttcaat
    24781 ggcagtagct agagcagctg ctgatgaaat aggtttacca ttattccaat acttaggtgg
    24841 agtaaatgct aaacaattac ctgttccaat gatgaacata ttaaacggtg gagagcatgc
    24901 tgataacaac gttgatgttc aagaatttat gatattacca gtaggagctt gttgtttcaa
    24961 agaaggttta agaatgggag cagaagtatt ccattcatta aagaaagttt taggagaaaa
    25021 aggattagct tgtggtgtag gtgacgaagg tggatttgct ccaaacttag gttcaaacag
    25081 agaagcttta gaattaatag ttgaagctat aacaaaagct ggatataagc caggtgaaga
    25141 tgttatgtta ggtcttgacg ttgctgctac agaaatgtac aacaaagaaa ctaaaaaata
    25201 cgttttagca ggagaaggaa aagagttaac tgctgctgaa atggttgctt tatatgaaga
    25261 ttggtcaaac aacttcccaa taataactat agaagatggt ttagacgaag aagattggga
    25321 tggatggaaa ttattaactg aaaaattagg taataaatta caattagttg gtgacgactt
    25381 atttgttact aacacagaga gattagaaaa aggaattgaa aatggagtag ctaactctat
    25441 attagttaaa gttaatcaaa taggtacaat aactgaaact ttagatgcta tagaaatggc
    25501 taagagagca ggatatactg ctgttatatc tcacagatca ggagaaactg aagattctac
    25561 tatagcagat ttagctgttg cagtaaatgc tggtcaaata aaaactggtg ctccatcaag
    25621 aactgataga gttgctaagt acaaccaatt attaagaata gaagaaatgg ttggagagca
    25681 agctagatat tgtggattaa aatctttcta taacttaaaa aaatagttat taaaatggtt
    25741 actaaaaaag attaatgaag agagaatata agatataaaa gagataagtg ttaattgatt
    25801 tttcaattca cttatccttt tttatatttt attacttaat tttattcata gattcagaaa
    25861 aaatatgaat aaatgtttct atctcatttt tatctaattg cataggaggt ttttattaaa
    25921 aaataaaagt gaagacatat tttgatttaa tcataattgt cattgaagta ttattgctaa
    25981 atgtggatta tttattttaa attatgttga aattaaaagt tatatcaata ttttaaaatg
    26041 attgttttat tttggtggct atttataata tatacaatga gagtagatta ttttaagtgt
    26101 taatttatgt attaatttaa atagttttct cttctaagtt ttattcattt
//
LOCUS       DAELNY010000039        25890 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.039, whole genome shotgun sequence.
ACCESSION   DAELNY010000039 DAELNY010000000
VERSION     DAELNY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.039"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1172)
                     /locus_tag="KPE55_001390"
     CDS             complement(<1..1172)
                     /locus_tag="KPE55_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0492481.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKN"
     gene            complement(1265..2137)
                     /locus_tag="KPE55_001391"
     CDS             complement(1265..2137)
                     /locus_tag="KPE55_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG0492482.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(2239..2574)
                     /gene="fliT"
                     /locus_tag="KPE55_001392"
     CDS             complement(2239..2574)
                     /gene="fliT"
                     /locus_tag="KPE55_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG0492483.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            complement(2577..4100)
                     /gene="fliD"
                     /locus_tag="KPE55_001393"
     CDS             complement(2577..4100)
                     /gene="fliD"
                     /locus_tag="KPE55_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG0492484.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSNEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKATYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     ITDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(4121..4477)
                     /gene="fliS"
                     /locus_tag="KPE55_001394"
     CDS             complement(4121..4477)
                     /gene="fliS"
                     /locus_tag="KPE55_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0492485.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            complement(4477..4872)
                     /gene="fliS"
                     /locus_tag="KPE55_001395"
     CDS             complement(4477..4872)
                     /gene="fliS"
                     /locus_tag="KPE55_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0492486.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DSVLPVIEQIKDTWHEADKKSKEERSRDK"
     gene            complement(4894..5106)
                     /gene="csrA"
                     /locus_tag="KPE55_001396"
     CDS             complement(4894..5106)
                     /gene="csrA"
                     /locus_tag="KPE55_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG0492487.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            complement(5100..5492)
                     /locus_tag="KPE55_001397"
     CDS             complement(5100..5492)
                     /locus_tag="KPE55_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG0492488.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            complement(5508..6440)
                     /gene="flgL"
                     /locus_tag="KPE55_001398"
     CDS             complement(5508..6440)
                     /gene="flgL"
                     /locus_tag="KPE55_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG0492489.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(6459..7769)
                     /gene="flgK"
                     /locus_tag="KPE55_001399"
     CDS             complement(6459..7769)
                     /gene="flgK"
                     /locus_tag="KPE55_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG0492490.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(7787..8203)
                     /locus_tag="KPE55_001400"
     CDS             complement(7787..8203)
                     /locus_tag="KPE55_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG0492491.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            complement(8208..8483)
                     /gene="flgM"
                     /locus_tag="KPE55_001401"
     CDS             complement(8208..8483)
                     /gene="flgM"
                     /locus_tag="KPE55_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG0492492.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(8635..8949)
                     /locus_tag="KPE55_001402"
     CDS             complement(8635..8949)
                     /locus_tag="KPE55_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0492493.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            complement(8973..9401)
                     /locus_tag="KPE55_001403"
     CDS             complement(8973..9401)
                     /locus_tag="KPE55_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG0492494.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(9410..10078)
                     /locus_tag="KPE55_001404"
     CDS             complement(9410..10078)
                     /locus_tag="KPE55_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0492495.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(10108..11091)
                     /gene="rfbB"
                     /locus_tag="KPE55_001405"
     CDS             complement(10108..11091)
                     /gene="rfbB"
                     /locus_tag="KPE55_001405"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG0492496.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            complement(11129..11686)
                     /gene="rfbC"
                     /locus_tag="KPE55_001406"
     CDS             complement(11129..11686)
                     /gene="rfbC"
                     /locus_tag="KPE55_001406"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG0492497.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            complement(11735..12604)
                     /gene="rfbA"
                     /locus_tag="KPE55_001407"
     CDS             complement(11735..12604)
                     /gene="rfbA"
                     /locus_tag="KPE55_001407"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG0492498.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            complement(12713..13585)
                     /gene="rfbD"
                     /locus_tag="KPE55_001408"
     CDS             complement(12713..13585)
                     /gene="rfbD"
                     /locus_tag="KPE55_001408"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG0492499.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENTEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            complement(13791..18872)
                     /locus_tag="KPE55_001409"
                     /pseudo
     CDS             complement(13791..18872)
                     /locus_tag="KPE55_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(18883..20415)
                     /gene="purH"
                     /locus_tag="KPE55_001410"
     CDS             complement(18883..20415)
                     /gene="purH"
                     /locus_tag="KPE55_001410"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG0492500.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(20408..21001)
                     /locus_tag="KPE55_001411"
     CDS             complement(20408..21001)
                     /locus_tag="KPE55_001411"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG0492501.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(20995..22071)
                     /locus_tag="KPE55_001412"
     CDS             complement(20995..22071)
                     /locus_tag="KPE55_001412"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG0492502.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(22065..23432)
                     /gene="purF"
                     /locus_tag="KPE55_001413"
     CDS             complement(22065..23432)
                     /gene="purF"
                     /locus_tag="KPE55_001413"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG0492503.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(23444..24142)
                     /locus_tag="KPE55_001414"
     CDS             complement(23444..24142)
                     /locus_tag="KPE55_001414"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0492504.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(24148..24624)
                     /gene="purE"
                     /locus_tag="KPE55_001415"
     CDS             complement(24148..24624)
                     /gene="purE"
                     /locus_tag="KPE55_001415"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG0492505.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            25074..25586
                     /locus_tag="KPE55_001416"
     CDS             25074..25586
                     /locus_tag="KPE55_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0492506.1"
                     /translation="MLEVIKNRHSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
BASE COUNT         7707 a         4811 c         2757 g        10615 t
ORIGIN      
        1 ctattttttt caattttaga atttgaaact tctttataat aattttttat atcttcaaat
       61 ctattcaatt tatataggga cataaataag ctaaagtaca cattcttttt cttttccaca
      121 tcttcaatgt tattgtactc tgaaattaca aactcatatt tttcaagctt ataataaatc
      181 tttatcagtg taataatagc ctcatctcta agccctacag tatcagtgtc tgaaaataaa
      241 ctatttgctt gtgtacttat ttcataatta tctaacaagt aaatatatct cttatacgaa
      301 tctatactat tttcatactt tcctaaatct acttgtgctt gtgcaatata ataatatata
      361 tcaatattat tcatgtcttt cttaatatac tttaagcaca atctttcaca cttcttatgt
      421 tttcctaaat ataaatatgt ttttacaagg cttgttatca agtaaccagg tgccaattct
      481 agtttagaat ataattcata agatttctca agatagtata aagcctcctg atattttcct
      541 aatataataa aatttttccc taattgatag tttgtatatg gagaattatc attctctttt
      601 aactcttgaa ataatagttt ttcatttctt ttcaccttat ttaatcttat ttcttcatct
      661 tcaaacaaat atccataatg ttcaaaatca gcaacattat taaatatagg gtttttgtat
      721 attggttgtt catgtacttt accatggtat ctaaaatcaa cgtttttaaa aagtctaagc
      781 actgaacaat atccatataa ttttttatca tatgagtaaa gattttttaa ttgtattgaa
      841 gcagaattaa attttttatc aagaccatct ttaaaaaatt ttacaatagt tgaagaatca
      901 attagtattt catctgcatc taatataagt aaccattcac ctttagcata ggatattgat
      961 atatttctca tactagaaaa atcattattc caattatgaa aatacacttt ttccgtgtac
     1021 ttcttagata tttttatagt atcatctgta gaacctgtat cgactattat aagttcactt
     1081 tctatagaat ttcttaatgt ggttaatgat tttaaagttt tttcaagtat tttttcttca
     1141 tttttaacaa tcataactat actaagtaac atataaattt tcctttcaaa agtatatttg
     1201 taaaaaaaga aaggagtaaa gcaaagcctt accctttctt ctatatccaa tatacaaaat
     1261 tattttatcc taataattgt aaaactcctt gtggttgttg attagcttga gcaagcattg
     1321 actgtgatgc ttgaactaat atattcattt tagataagtt aaccatttct gaagcaacat
     1381 ctgtatctct tattcttgat tcagctgctg taacattttc tatagtatta tttaagttgt
     1441 tttgagttga ttctagtcta ttttgttgtg cccctaattt tgctcttgat gtatttaatt
     1501 ctttaagagc tacatctaag ctagatacca tttgtttagc tgcatttgtt ccagaagttc
     1561 catctgcatt taagcttgct attgtagaac ttgttatacc tgctttactc attatacttg
     1621 aagtatttac tagagcaact tttatttcat tgttgttgtt agatgttcct gcaacatttg
     1681 ttccaaagtt tgctccaact tgtaatctta tttctgtaga acttccatca agtaatttct
     1741 taccattaaa ctcaattgag cttgatattc tttcaacttc atcttttaat tgtagtaact
     1801 catcagctat cttttgtctt tcttcagcag tattagtttc atttgaagat tgtactgata
     1861 aagttctcat tctttgtagt atgttaccag tttcttctaa tgcaccttct gctgtttgta
     1921 caacagatat accatcttga acgtttcttc ctgcttggtc taaacctttt atttgagctc
     1981 tcattttttc agatatagca agcccagcag catcgtcagc agctctctta attctaacac
     2041 cagaagatag cttctccata gacttacttt gtgcatttac gtttcttccc atttggttat
     2101 ttgctatcaa agcacttaca tttgtattaa ctctcattat tttaccctcc ttagtatagt
     2161 tgacatcctt gtcatttcta cttttaatct tttttattaa ttcttcattt atataatcgg
     2221 acaagatggt acataacttt atactttttt attaaaaata tttacttttt ctctatttaa
     2281 gttataatac attgtgttca cttgtttagt aagcttatac tcatgtaatt ctttttttac
     2341 ttctaataat tgtccttgta aaatatctct tatcctgcta tcaatttcaa ttaattccat
     2401 tctattataa agctgtataa aatcatttct atcaatttca cttacactat ttataatctc
     2461 ttgtctttca cctaacttaa tacttatctt tatatattct tccttttcaa tcaaatttat
     2521 aatttgtaaa gatatatctt tatataaatt taatttttct tttaatttat ccataattaa
     2581 ttaccctgtg cttgtgagaa ataattcatt tgggaagaat actgattcaa caaagattct
     2641 aaggctgaat atttagcata aagtcttttc tctttatcat tcattttttc aactaacctg
     2701 cttatatttc tttcttgctc tgcaatttgt tttgaatata cattttcact agcagaagca
     2761 gttttttcta tacctgcttt tttaggaagt ttagaagttg aacttccaga atattcatat
     2821 acaatatctt ttagcttttc aaacattccc ttatcatcat aattcgaaga ataacctgct
     2881 agtgttttgt ataccttatc actatcatct ataagagcct ttgaaaaagt atctgcatta
     2941 aatgatattt gacctttttt attgtaattt tcactttcat tgatacctag ttttcttaat
     3001 gcaataatat tttttccatc tccaaaaaat gcagactgaa tatcttcaac aaaacctctt
     3061 aactcatcat catttctaag tataccttct ttagctttct tttcccattt ttctatttct
     3121 tcagttgtca tatcttcttt ttgggcatct gtaagaggtg gataatcttt tggcttttta
     3181 gtagtaacta aaccatagac cttgtccatc agtttattat aatcatccac aaatgcttgc
     3241 atatttttaa cagcttcttc agtatcagtg atagaagtca actttgcagt tccttttgaa
     3301 tttacattat agtcaatatt atctatatta aatgaatttt gttctttact tatatgagtt
     3361 actcttccat gttcatcagt tacatcagct tctagattct ttccatatac acttgccact
     3421 ttgtttccac cagcatcatt tataaatgtg ataccattat cattggcaat ttgctgagcc
     3481 aaattgtcat ttccaattac tcttaaatta atgtctgatg acgaaccagt ctgttttctt
     3541 gaaataatca attcacctgt catctcacta taagtagcct ttatatcatt atctgcgcaa
     3601 agtgagttta tcttacttac aatagtactt gtagtatctt gttcagatat tggtatattt
     3661 acatctccta tagttaaaga actagctcca ctagcatttg gagggaattg tcgaacaata
     3721 ggttcatttg aatttagttt aatagtaaat tttgctggtt cagccatttg agaaactgca
     3781 aattgataat ttattttttc tgcaccagct gaaccttttg ctactataat actttcatca
     3841 gaactggtta ttcttgtaga agagtatgct ttttcactta ctatactatt aggagaattt
     3901 acgcttagat atttatcata aagatctttt gattcttgta taacatttct atatatttct
     3961 tgcttccatt taacgatttg ttgttcttgt tttgctttat caactttttc cttgtctcta
     4021 atcatactag cttcgattat gccttccata tcaaaatttc ctgaaagacc tgtaactctt
     4081 ataggactta tacttgacat acctttatcc ctccatctaa ttatattttt agttgattgt
     4141 aaacttcatg ccatgtatct ctaatttgtt ttactattgg caatatatca tcaattattt
     4201 taatatcttt attttcatca gctattgcta attcagattt tataaattca taaactttat
     4261 acatatcctt tgcccattgt cctgcatttt ggtctaatgt agtcattagt tctgtaaata
     4321 tattttgaac tcttacaagt tcattatgtg ctttcaaata gtcgttattt aaaagagctt
     4381 ctttggatat attggtatat tttactgtgc catctacaag catcaatagt aattcctctt
     4441 ttgataactt atctaattca gctaaattta attccattat ttgtccctac ttctttcttc
     4501 tttacttttt ttatcagctt catgccatgt atctttaatt tgttctatta caggcaacac
     4561 actatctatt acctggatgt cttttctaat attggctctt gaaagttcat atcttacaaa
     4621 atcataaact ttgtacatat cctttgccca ttgtcctgca ttttggtcta atgtaaccat
     4681 tagttctgta aatatatcct gaactcttac aagttctcta tgcgctttct gtgtattttt
     4741 atctaagata gctgctctgg ctattttggt atattttact gctccatcaa caagcataag
     4801 taacaattgt tcctttgatg ccataaaaac agcattttgt ttataagaat tataaggatt
     4861 ttctccatac atatattact tcctccaagc ttattatttt aatgacttta aaatttttat
     4921 atttttattt gtagctttta tattctcact ctttactttt tcgtatattt cttttcttaa
     4981 tatacttata ttgttaggcg ccgatatagc tagtttcaca ttgtttccat ctacccctac
     5041 aatttttact tctatgcttt ctcctataag tactgcttca tctttttttc ttgaaattac
     5101 tagcatccat tatcacctct caataatgga tgccttattg aatatttatc atcctgtaaa
     5161 attatttgca ttccaaaatt agtttttata tttatcacta taggagcttt catgttcaca
     5221 gttgattctt caaatgtctg accaagagta attatattaa atagtacaac atcctttgga
     5281 gatttcactt ctagtttttc tattgtttcc tgatttaaaa ctatttcata ttcattgata
     5341 atctcaaatg gagatacaac aacaaatcct ataccttctt cttcagtaga attaaattct
     5401 tttaaagaat cattatcttc tatatctaaa agctcatatt cttttaaatt ttcaaaacct
     5461 aatatacctt tttttaatgt aaccttcatc atatctgcgt tattttacta tatgtaatct
     5521 aatatagata cattaaataa ctttcctcct gtttgtagtg aagcttgata agttaactct
     5581 gctgccttta attgaacaag tttttcacca taatctattt cttcattttg tgacaaaata
     5641 tcctttaact gtatcaaact atcatcgttt ttttccttca tagcctctac tgtatttgct
     5701 tttgtcccat atatagacct aacatttact gtattttcta ttagttgttc catatcgccc
     5761 aaacaagttg ttgtaagctc ttttatagca tcagcatctg gatttgctaa tttttttgat
     5821 atgttattta actcttcaaa tgggtcagtt tttgttgggt caaatccaag gctactcaat
     5881 ttaacgttat aatctacact tattccttga gctatatcca tactcaaaga gtcatttaat
     5941 ttattaagat ttagactaga attaaacttt aaactcactg aaccatcagc attatctata
     6001 cattcaacag gtggtacccc cgtatttgaa cctgaaaata tgtacttccc ttcatgtgtt
     6061 gcatttaaag catttgcaat ttctttcatt ttttcattag tttcattact aagagtcttt
     6121 aattcatcat ctgagtatgc accatcacca cttgccacca aagttttctt tatgtctgat
     6181 aataattttc caagctgatc taatgcagta tcagtagttt ctaaccaatt ccctgtatct
     6241 ttacaattct caacataagt ctccatagtc tttatttcac tttttgagtt taatatcttt
     6301 attgctttat atggattatc tgatagcttg tttatttctt ttgttgtatt aacttgtcta
     6361 ttaaccttat ccaatctttg tagattatct tgtagtgagt ttagatagct gctagacatc
     6421 atacctgtac tgactctcat tataaattcc cccttctatt aaattattcc atttattaca
     6481 acatccaata gttgcacagc tgttgacata acttttgcac tagcttgata tgaacgttgt
     6541 atttttataa gctctgtcat ttcttcatcc aaagatacac ctgaaacact cattcttgag
     6601 ttatcaatat tatttataat tttacgttga tttgcttcat cttgatttac tttttgagaa
     6661 tctaaaccga ttgattctgc aaaggcttta tagtggtcac ttattgtact tacttttcca
     6721 ccagccaccc caatatctat tttttcatcc tttaacttag caatttttga agcttcatca
     6781 gcagtaagct ttaattcatt tgggttactc tttatagctg gatttatctt tataatatct
     6841 tcaccatcta catttgaagt tataaaaaaa tctgttactg ttattcctgc agacgtatta
     6901 taaacatcat ttatctgttt agctaatcca tcagatacat cttttaaatc gcttttatat
     6961 gtatcaatct taccatgcat ttcaaacaag ccttgtaagg tccctgatat acctgttttc
     7021 caagttccat ctggattttt taattcatta agttctaatt taccatctgc aagcatattt
     7081 ttaacatcac tattttcaaa gtctagttta aaactcaagt tatctaaaat tctatctctt
     7141 tcatcaagta aatcatttgg tgtagagcca gaaccagata taatatcaat agatttattt
     7201 aaatcttcaa gactttttaa catgtcattt atttgtttta tctcagtgtc tatattagaa
     7261 tctatatctt cttttaactt atcaagcttc ttataacttt cacttattaa agttgctaaa
     7321 ttagatgcgt tttctaccac tagacttttt ttactgtcac tatttatatc ctttgataat
     7381 gactcccatc ctttaaaaaa atcatctata cctgcagata ttccatagtc tgatggttca
     7441 tttagtatag tttctatagt attgtaataa tcatattttg aaactgtctt accatactta
     7501 tgagcttctg ccctaaattg gtaatcataa aaactatttc tagctcttgt tacatccgtt
     7561 acatgtactc cagtccctaa ttgtcctctt gcaattgaat tactcaaact aggcatatac
     7621 attggactat ttgtggcaaa ctcggtcctt tgcctagaat aaccgtctgt atctagatta
     7681 cttatattat gagaaattgt ctgtattgct ttttggtgcg cattcattcc tgaattagct
     7741 gtatttaaaa tggaaaataa tcctgccata tttctcctcc taatatttat tttctaatat
     7801 ttccataaga attataagtt gtagtttgct tatcagctgg ttttatgaaa ttaatcattt
     7861 ttttcgtaaa aataagttct ttctttatta gagaatcatt tgattcttgt tgatttttag
     7921 ctaaagcaag aatgtgtttc aagtctgtat atatttcttt taagtatcca tcattactat
     7981 tttcaacaac ttcactcatt gaaatatcat caccaaaaag ttgtcttctc tttatttcta
     8041 tggatgcaag ctccctgcta atatcttcta gtttaggatt taacatattt aaattttcta
     8101 agtctttttc aactatataa tcaaattgtt cttttaataa ttccatcatc ttactaatta
     8161 tatttttttc ttcatatatt ataactttta agtctgtaat catgacttta tcctcgcata
     8221 tcctctatca tttttttcgc aatttctttt ggttttattt tgtaagttcc attttctaat
     8281 gcatttttta tttcatcgac ttttttattt ctaatattct catcactcat atttgacaca
     8341 cttgccattt cctttgcaaa tttagataac tctatcttgt ctgttttatt aattttttta
     8401 acctcttcca ctttttttat accagatgta ttctttaaat aattattcac tacaatattt
     8461 gaacttacat ttttaatatc catagtttca cctccaaaat tacatactta taatttagtt
     8521 atcgctcata aaataaaaat atttatataa ttcacacata ttttaaattt tttataaaaa
     8581 tatcccctta gctaaactca tacagaatag cctaaaggga tactatttaa aatgctaatt
     8641 tacatctgga tcttctattt tagataatct tacagataac ctctcatttg taataatgct
     8701 ttctccataa gcaaacaatc tatcgtttgc atatatatct aatttttcat ccacattctt
     8761 acttaatttt attatagagc cttcttttaa atctaatata ttttttattg tttctgttgt
     8821 atttcctata gaaacagcta catttacttt tgcattaaaa acattttgat tttcttctat
     8881 aacaccagat ttttcttcta ttaattgccc aaaattcaat tcatatatat cctctttttt
     8941 gctattcata aaatttttct ccttgtataa ccttatctat acatatctat caataagcct
     9001 tgtatcatgt caacagtaga aacaatatct atgttttctt tttcttcact aattaactta
     9061 ctagtcatat cctcaagttt actatcaaca atctttacat tcttataata ctgatgctcc
     9121 caaaagctac tatttttatc aagtgaatac atattatcta caacagattt taggtattct
     9181 tttactgcat ttttatatat actaatatcg ccatagtttt gagtaagtac aagcctttct
     9241 cctgcctttt ttacactttc caaatttcgt tttatatctt ccaatgaaga agattgtttt
     9301 aaagagttta atttgtcaga aaatttatta ctctctttta gttttttaac ctccatattt
     9361 atacttggac ttatgtttag cttatctata tttattccca taataaagcc taccctctat
     9421 aatatttcat aaccttagga atataatttt gtgtttcttt tggcatttta tacaggtcag
     9481 agattgattc tactcctcta cttttcattc ttccaggacc accattatat gccatcaatc
     9541 ccatttctat gctccctcca aacatatcaa tgtattcctt aatatgtcta gttccagcat
     9601 ctatattctg ttctatatta aaagggtctg taactcctaa gtctctgcag ttttctggca
     9661 taagttgcat aagaccctta gcaccagcag aagaaacggt atttggatta aaatctgatt
     9721 caactttaat tattgctttt ataagatttg aatctacgtt atattttttt gatgcttgat
     9781 ctacagcatt ttcaattctc aactttacat ttttatcttt tgtatcaaca tctataaatc
     9841 tactagtggt attatttact acattatcaa gttcattaaa taaattctca gaattagaca
     9901 ataaattgta gctattagaa gtatgttgat tattttgtga taatgctttt aacaaggtca
     9961 tcataatcat atcaaatcca gatgaattat tatatgtaca attacattga tttgtagtat
    10021 tactaccaga taattgagtc aatgctaata tattaattag tgaacttatc tcattcaata
    10081 taaaacgccc ctttctaaat tttaaagtta tactgataaa acagctttcc accaagattc
    10141 attttctata taccacttaa cagtttcttt tatacccaaa tcaaagtcat attttgcctt
    10201 ccaacctaac tcattctcta ttttagttgc atcaatagca tatcttaaat catgaccagg
    10261 tctatcattt acataggata ttaactcttc agatttacct aaaagattta atatcatttt
    10321 tactacttct agattacttc tttcattatg acctcctata ttgtaaatct cccctatatt
    10381 tcctttatgt attattaagt ctatagcagt acaatggtca tatacatgta accaatctct
    10441 tacattttcc ccattcccat atactggcaa ttgtttgttt tccaaagcat ttaaaatcat
    10501 aagaggaatc aatttttcag gaaaatgata tggcccataa ttgttggaac acctagatat
    10561 agttgttaac aatccatatg ttctataata agaacttacc aataaatctg ctgatgcttt
    10621 agaagctgaa tatggagaac ttggattgat agaagattgt tctgtaaaga ataaatcctt
    10681 tctatcaatt ggtaaatcac catacacttc gtctgtagat atttgatgat accttttaac
    10741 tccatatttt atagatgcat ctaaaagtac ttgtgttcct ataatgtttg ttttaataaa
    10801 taaatcagga ttctcaactg acctatctac atgagattca gcagcaaagt taacaactat
    10861 atcaaacctt tcttcttcaa atagcttata cacctgctcc ctatttgaaa tatctccttt
    10921 aacaaattta taattggaat tgtcttgtat ttctattaat gtctctaaat taccagcata
    10981 agtcaacaag tctaggttaa caataaaata atctacatac ttgttaagca tataatgtat
    11041 aaaattactc ccaataaatc ctgctcctcc agttatcagt atttttttca tattttttct
    11101 cctaaatgtt aattctaata aatttttatt aacgtcttgt acaaatatct ttaaaacttt
    11161 tttgtttttt gtccttttct gataaaaata tttcttcaat tccttttaaa ggccactcaa
    11221 tagatatttg cttgtcattc catataaagc cattctcaaa ttctgggtaa taataatttg
    11281 tacatttgta actaaatact gctttttctg ataaaactaa aaatccatgt gcaaaccctt
    11341 ctggaatata aaattgtttt ttattcttat cagacaatat tacaccttcc catttcccaa
    11401 atgtttgtga atctttcctt aaatcaactg caacatcgaa aacttctccc tctgtaactc
    11461 tgactaattt tccttgagga tatttaacct gaaaatgcat tcctcttaaa acgccttttc
    11521 ttgagctaga ctcattatct tgaataaagc acatatcaag accctgctct ataaaatcct
    11581 ttttattata gctttccata aaatatcctc tttcatcata aaatacattt ggttcgacta
    11641 tatataatcc ttctatttta gtctttataa cattgatttt tcccattata ataaactccc
    11701 ttaataaaat ttataataaa ttttatctac catattacat atcttcacta gccaatttta
    11761 atagatattg accatattca gtttttatca gtggcttggc tagtttcatt aattgttctc
    11821 tacttatata tccttttgta tatgcaatct cttctaaaca tgctatatac agtccctgtc
    11881 ttgtttgaac cacttcaaca taattagaag catccaatag acctttatgt gtaccagtat
    11941 caagccaagc catacctctt cctagaagtt ccacttttag ttttcctcgt tttagatatt
    12001 cactgtttat agaggttatt tctaattcac ctctatctga cggttcaact agctttgcta
    12061 tctctacaac atcattatca taaaaatata atcctggtat agcataatta gatttaggaa
    12121 ctgtaggttt ttcttctatt gacaatacat taaaatcctt atcaaattct acaactccaa
    12181 acgaatttgg attacttaca tgatacccaa atatagttgc tccttttctt ttaactgcat
    12241 tctccaacct ttcactaaat ccatatccat aaaatatatt atcccctaat acaagagaca
    12301 ctctgtcatt tcctataaat tcttctccta ttataaacgc ttctgcaatt ccatttgctt
    12361 tctcctgaat tgcatatgtt atgttaagcc ctagtgaact tccatctcca aataattctt
    12421 caaatgcttt tatatctctt ggtgtagata ttattaatat atcttgtatc cctgccaaca
    12481 taagaaccga catagggtaa tatatcattg gtttatcgta tattggaagt gtttgttttg
    12541 atatacattt tgttatagga tatagtcttg taccagaccc tcctgctaaa ataattcctt
    12601 tcatcttttt ccatccttaa atttgttttt gtcatattat gattatcgga taaataaaag
    12661 aaatatttat attttttata atatcttatt ttcttttctc aaaataatac atttactttt
    12721 catttatatc atatctttct aaatattctt caatggattc ttcccacttt ctaaaagaat
    12781 ttaaaccaat taacttcaac ataaaattat ccaatacaga atatagtggt cttggagcct
    12841 tacatttaaa ttcgttactc ttaattggat ttacttttat atctatattt tttagtttaa
    12901 atatcttttt ggcaaaatca taccaactac actcaccttc acatgtagca tgataaactc
    12961 cataatgttc tgtttccatg atttttatta taatttttgc taagtctact gtacttgttg
    13021 gagttccaaa ctggtcatta accacattaa cctctgtatt ttctaaagaa agttttatca
    13081 tggtctttac aaaattattc ccattaccat ataaccatgc tgttctcaat ataaagtact
    13141 tatgtgagaa ttgttcaaca aatttttctc ccataagttt actttttcca tatacagaat
    13201 tgggttcagt tttattgtct tccctatatg gatatttaga aaaaccattg aatacatagt
    13261 cagttgatat gtgaataagc ttagcattaa ctttttctga tgctattgct agatttctca
    13321 ctcctaaagc atttattttg tatgcaattt cgatattatc ttcacatgca tcaacctttg
    13381 tatatgctgc acagtttaca actacatcag gtttattatg caaaataaaa ttatctacat
    13441 tagtctgatt ggatatgtcc aaatcacttc ttgttgtagc taatataaca tattttggtt
    13501 ggtttatact ttgattgatt gcttctaatt ggttaaccaa ttcttttcct aattgaccat
    13561 tagaccctgt tattaatatt ttcattaatt atgttgccct ccaataaata ttcaatttta
    13621 cttaataatt aattataaca cagcttgtat tttaacttag gttgaactta ttatttctta
    13681 gataaataaa aaagagatgt aatattaaat gattatcatc atattattac atctctttaa
    13741 atctaaatat ctaaattata tattctagct atcaattaaa catcaaagct ttatctaaaa
    13801 tatttaactc cagattcaaa tatcttttgg tctttttcac ctataatatt cttatatact
    13861 tcctcaccaa ttctttcaga atgtcccatt ttaccaaaca ctctaccatc aacactagtt
    13921 ataccttcaa cagcatagaa tgaaccattt ggattaaact caatatcata agtagcttca
    13981 ttattaaagt caacatattg agttgctact tgtccattag caattaattc tctcataaca
    14041 tcttcactgg caacaaactt accttctcca tgagaaattg ctatattatg aatatctcca
    14101 acattagttt gagctaacca aggagattta ttagatgaaa ttctagttct agcaatctta
    14161 gcttgatggc gaccaatatt attgtaagta agtgttggag cactttcact tggaactctt
    14221 atttctccat atggaactaa ccctaattta attaatactt ggaatccatt acagatacct
    14281 aacattagtc catctttttg agttaaaaat tcatttatag cttcttgcac tctaggattt
    14341 ctaaatacag tagctataaa tttaccagaa ccatctggct catcaccagc actaaatcca
    14401 cctggtaaca ttattatttg agaagatttt atttcgttta ctattgtatc tatagaatct
    14461 tctatgtctt tataagttaa attcttaaat actcttatac ttgcattagc tcctgctctt
    14521 tcaaatgctc ttgcagagtc atattcacag tttgttccag ggaatgctgg tataaatact
    14581 cttggagtag ctatacttaa tgatgatttt ttagcttctc cttgtgatat ataacttata
    14641 gtttctatct ttgattttac ttcttctgtt ttagttggga atataggctc taaagtacta
    14701 caatgtgctt tatataattc atctaatgca agttcttcac ctttgataaa tatactagct
    14761 tctttaactg tacttccaag cacaacataa ttgtatcctt ctaacatact taaatcatca
    14821 tttgctaatt caagtactat atttccataa ctagcctcaa ataatttatc atctgtaaat
    14881 gctttttcag cctcttcact aaacttaaat cctattttat taccaaaagc cattttactt
    14941 attgcttcac ctacacctgc aaatcctaat gcatatgttg ataaaacttt atttccatgt
    15001 ataagttctc ttactttatc taagtttctc tttaattcct caaaatctac aacatcattt
    15061 tctgccttgt ttacacataa cattactact tttgagtctg cttttttaaa ttctggagat
    15121 actactttct tcgcatctac tgtgtcaaca gcaaatgaaa ccaatgttgg tggaacttca
    15181 atatctttaa atgtaccaga catactgtct tttccaccta tagcaggtat ctcaaattta
    15241 ctttgtgcat aaaatgctcc taaaagtgct gagaatggtt taccccattt ttctggattg
    15301 ttaccaagtt tttcaaaata ctcttggaat gtaagtcttg ttgtactata atttcctcca
    15361 atagcaacca atttacacac agattctact actgcataca aggcaccatg gaatgggctc
    15421 cattttccta cctttggatt gtatccataa gtcattattg tagaagtatt agtctctcct
    15481 ccaagtactg gtatcttagc aaccatacct tgtgttggag tcgcttgata ctttcctcca
    15541 aatggcataa gtactgtatt accaccaatt gtattatcaa acatctccac taatcctttt
    15601 tgagaacaaa cattaaggtc agatagtact tttgtaaact tgtctttcat acttgcacac
    15661 ttaacatctt ttactatttc attagagcta aagaatgtat tttcttcatc tactttagta
    15721 acatgaacat cagtagtttg ttttactccg tttgtatcta aaaactctct atttatatct
    15781 acaattgctt ttccattcca gaacattctt aaatatcctg tatctgttac tgtagctaca
    15841 tgcgtagctt ctaagttttc ttctactgct aattgtataa atttatcttt attttctttt
    15901 tttatggcta ctgccatacg ctcttgtgac tctgaaattg caagctcagt tccatcaagt
    15961 ccatcatatt ttttaggaac taagtctaag tttatatcta agctatcagc aatctctcct
    16021 atagcaacac aaactccacc tgctccaaaa tcattacatc tttttatcat ttgagcaact
    16081 tctttatttc taaagaatct ttgtattttt ctttcattag gagcatctcc tttttgaact
    16141 tctgcactac aagttaagat tgactcttca ctatgttctt tagatgaacc agttgctcct
    16201 ccacagccat ctcttcctgt ttttcctcca agaagaacga ttacatctcc tgcttcaggt
    16261 ctttctctta ctacattctc ttttggtgct gctgctataa ctgcacctat ttccattctc
    16321 tttgcaacaa agttttcatc atatacttct gcaacttgcc cagttgtaag acctatttgg
    16381 ttaccatatg aactatatcc atgagcagct tctgtagtta tttttctttg cataagtttt
    16441 ccaggtaaag tatcttctaa agtcgttctt gggtcagcac ttcctgtaac acgcattgct
    16501 tgataaacat aacttcttcc tgataatggg tctcttatag ctccacctaa acaagtagct
    16561 gctccaccaa atggttctat ttcagttgga tggttgtgag tttcattttt aaacattact
    16621 agatattttt ccatcttacc atcaacttca acatctacgt ttatactaca tgcatttatt
    16681 tcttcacttt catctaggtc atttaatttc ccatttttct ttaattcttt tactgcaaca
    16741 gtggctatat ccattaaaca tatatcttta tgtctgttca catatacatt gtctcttgag
    16801 ttaagataca tttgatacgc ttcttttact atatcattaa acttaccatc ttctatcttt
    16861 acattttcta tttcagtcat aaaagtagta tgtctacaat gatcagacca ataagtatct
    16921 aatactttta tttctgttat agttggattt ctcttttctg tatctttaaa gtatttttga
    16981 acgtgttgta agtctcctaa agtcattgct aatccttttt cacttacaaa tgttcttaat
    17041 ccattttcat ctaaatctat aaatccatct aatacttcaa ctgttgttgg tatttcagtt
    17101 tccattttta acgtttctgg tttctctaaa gatgcttctc tactatctac tggatttata
    17161 cagtaatctt ttattttaga aaactcttca tcagttattt tgcctgataa tatataaact
    17221 tttgctgtat taatagcagg tcttatccct tgatttacaa tttgtacaca ttgagctgcc
    17281 caatcacctc tttggtcgta ttgaccagga agaaattcta ttgcaaatac tctttcatca
    17341 tttaattttg gtatttcttc ataatatacc acatctaaat ttggttcaga gaatattgtc
    17401 tttgctgcat tttcataaac ttcttctgat attccttcta catcatatct atttaatatt
    17461 cttaaatttt ctatattatc tatgtgtaaa ctttctacta agtctttttt taaagcttta
    17521 gcctcaaggt caaaaccttc tcttttttca accaatactc ttctaaccat tgagtcttta
    17581 ttttcagtat tcaacatacc ttatcctcca ttttagaaaa attatcttcc tatgtctgtt
    17641 ctatagtgca ttccttcaaa gtttatcttc tttatgtttt cataaacttt ttctgctgca
    17701 tccttaactg tagttgactt tgcagttata ccaattactc ttcctccatt ggtaaccaaa
    17761 ttaccatcaa acatcttagt gccactatgg aatactacta tatcatcatc caagttttca
    17821 agaccagtta taattttccc tttttcataa ctgtctggat accctcctga tgtaagcata
    17881 acacacacag cttcatcatc actatagttt atttctatat cttttaactt attatctagt
    17941 atagcttcca ttatctcatg taaatctgtt tctaatctaa ataaaacaga ttgtgtctca
    18001 gggtctccaa atcttacatt atattcaagt actttttctc catcttcagt tatcataaga
    18061 ccaacaaata aaataccttt aaaattaagt ccatcttttt tgaagccttc taaagttttt
    18121 tctaatactg tctcttttac tttatttgct aattcttctg tatatatttc actaggtgaa
    18181 aaagttccca ttcccccagt gtttggacct tgctcatagt tgtttacttt tttatggtct
    18241 tttgcacttg ccataggaac tattgtatca ttgtctacaa atgctagaat tgaagtttct
    18301 attcctttta aaaactcttc aattactatt ttatctccag cagctccaaa cttcttatct
    18361 gacatcattt cttttaaagt tgctatagca tcttctctat tttcaggtat tacaacacct
    18421 tttccagcag ctaatccgtc cgcttttatt actactggat atccaaaact atctatttct
    18481 gatatagctt cttctaagtt tgtatattct ttatattttg cagtaggtat gttatgtttt
    18541 atcataaatt cttttgaaaa agctttactt ccttcaagtt gagcacattt tttattaggt
    18601 ccaaatactc ttaatccttc tttttcaaac tcatctacta ttcccataac tagtggtact
    18661 tctggtccta ctattgtcag acctatttca ttttctttgg caaattttaa aagttcttct
    18721 atattagtat cacctatatt tacacattct gctacgtttg caattcctgc attacctggt
    18781 gcacaataaa ttttttctac atttttttct ttgcttagtt tccagcatat agcatgctct
    18841 cttccgccac cgcctacaac caatatcttc ataatatcct ccttagtgtt tgaagtgtct
    18901 tattcctgta aataccataa ccatatcata cttgtcacaa gcttctattg aatctttatc
    18961 ttttatagag ccacctggtt gaactactgc tgatattcca tattcatgag ctaatgttac
    19021 acaatcatca aatgggaaaa atgcgtctga cgctaaaaca gctcctgtaa aatctttatc
    19081 tttgttattt tcaagagcat tttttaatgc ccaaattctt gatgtttgtc cacaacctaa
    19141 agctagagtt tgtccatttt ttactattgc aattgcattt gatttcatat tttttactac
    19201 tctcatacca aattccatgt ccttaagttg tgcatctgtt ggtttttcct ttgttacatt
    19261 ttcacttttt tctgctaata tattgtttct atcttgtatc aataattttc catctaaata
    19321 tttcatttca tatggttgta aactattttc tattttagct aattttaata ttcttagatt
    19381 tttcttttgt ttaagtatct ctaaagcttc tggttcaaaa tcatatgcta ctactatttc
    19441 taagaatatt tcatttaatt tctctgctgt agctttatct atagtagaag ttattcctac
    19501 tattcctcca aatattgaaa ccttatccgc ttcataacat tttgtatatg cttcaaaagc
    19561 atcttttcct aaacctaccc cacatgaatt agcatgtttt atagctactg aaactacttc
    19621 ttcactatct ttaaattctc tcattacttc taaacatcca tgtaaatcat ttaagttgtt
    19681 aaaagaaagt tctttaccgc ctaattgctc ataatttaag attggatttt ttgcatttga
    19741 ctgtgaatat aagaaaccat tttgatgtgg attttctcca tatcttaaag tttgttcttt
    19801 ttggaaagtt aaatttaata tgtcaggata cgtatctcca acttctccag caaaataact
    19861 agatattaac gcatcatatc ttcctgtagt tgagaaagct ttatatgata atttttttct
    19921 gtcttctaat gtcattgtat ctgtttttaa tttttctatt atagaatcat aatcttttat
    19981 atctactact actattacat ccttatagtt tttggcagca gaacgtatca tagatggtcc
    20041 accaatatct atattttcaa ttatttcatc atgacttttt ccagctttta aagttccttc
    20101 aaaatcatat agatttacag ctactaaatc tattgaatgt attttgtgtt catttacagt
    20161 ttctacatga gattctttat ctcttttata aagtatacca ccatgtatat atggatttaa
    20221 tgtttttact cttccatcta atatttctgg aaaatttgta acatcttcaa cttgcattac
    20281 attaacacca ttttctttta atgttttaaa tgtatttcct gtagatataa tttcataatc
    20341 taatttattt aactctttag caaattccac tacaccagtc ttgtcagtta cacttattaa
    20401 agctctctta ctcatttata aacactctcc taccttgaag ttttaattta ttttcacaaa
    20461 aaagacttat actttctttt aaaatcctat gttcaacttc taacactctt tttgcaagtg
    20521 tctttgcatc atcatcttga tttaccttaa ctacatcttg cattatgatt ggtccagtat
    20581 cagcaccttc atctacaaaa tgaactgtag caccagttac tttagctcca taatctataa
    20641 caccttgatg aactttttct ccatagaatc cagctccaca aaatgaagga attaatgatg
    20701 gatgtatgtt tatcattttg tttctaaact cgttaaccag ctttgggctg attattttta
    20761 aataaccagc caaaactact aaatctattt tattttcctt aagaatttct attatcttgt
    20821 cttcatcagt ttcacaaatt gcttttatgt tatgattttt agctctctct agcccataag
    20881 caccttgctt acttgagata actactttaa cctgaccttt tatttctcca gactcagtac
    20941 catctataac tgcttgtaaa tttgttcctc caccagatat taaaactcct atatttagca
    21001 taattctaca ccttcatgac tatctactac ttcaccaatt atataagctt tatcttctaa
    21061 taattcacta tattttttat ctacataagc agcattgtca ttttctctat tatttataaa
    21121 gtttacaaca tcatcagcat tttctttatc tactattaaa actagcccaa tacccatgtt
    21181 aaaactctta tgaagttctc tctcatcaac agcattaaaa ccttctatca ttttaaatat
    21241 aggaggcttc tcccaagatt ttttattgat atctagtcct aaccctttag gtataactct
    21301 tgttatattt tctataagcc ctccaccagt tatgtgagct atagctttta tgtcatgttt
    21361 agctaataca tctaaagcta atttaacata tattttagtt ggtgttaaaa gtgcttctcc
    21421 aactgtcatt cctagttctt caacatattg ttccatttta taattgtaag tttctaaaaa
    21481 tatctttctt ataaaagaaa atccattgct gtgtactcca cttgaagata taccaaccaa
    21541 tacatctcct gatttaacat tgtttccaga aacaatcttt tctttgtctg ctattcctac
    21601 agaaaatcct gctaaatcat agtcatcttc tccatacatt ccaggcattt cagcagtttc
    21661 tccaccaatt agtgcacaac cagacatttt acaaccatct gcaattccac caactatttt
    21721 ttcgatatgc tcaggcacta atttcccaag agctatatag tcaaggaaga ataatggttt
    21781 tgctccttga caaattaaat cattcacgca cattgcaact aaatctattc caacagtatc
    21841 atgcttgtcc atcatctgag ctatttttaa cttagtccca acaccatctg tagatgcaag
    21901 caaaactggt tctttcatac ctacaaaatc ttttaaactg tataatccac tgaagttacc
    21961 taaatctcca actacatttc catcataagt tccttttatt ttacctttta taaggtctac
    22021 tgctctattt ccttcatcta tatctacacc tgattcttta tatgttaaca tgaaagtagt
    22081 tcctccttat caattttctt aactgggtat tctccatcaa aacaagcttt acagaaagta
    22141 gacttatgtt ctgttgcatc taacattcct tctatatcta ggaattttaa actatcacat
    22201 ccaatatatt ctctcatttc ttctacgttg ttacttgatg caattaattt agagcgattt
    22261 ggagtatcta ttccatagta acaagagtaa gctactggag gagaagttat tctaagatga
    22321 atttcttttg ctcctgcttc tcttaaagat tttactaatt gtttagatgt agttcctctt
    22381 acaattgagt cgtctactaa aattatagat tttcctttta ttattgttga aagaggattt
    22441 aattttattt ttactgctat ttctctttct tcttgtgtag gttttataaa tgttcttcct
    22501 acatatctat ttttaactaa gccttcactt atttttaatc cactagcatt tgcataacca
    22561 atagctcctg gccaacctga atctggaact ggaactacca tatctgcctt tacagtttca
    22621 ttctgagcta atctctcacc acatttaatt ctaaattcat atgcatttac attgtctata
    22681 gttgcatcat ttcttgcaaa gtatatgtgt tcaaatatac aacttttctt aactggttta
    22741 taattttctg aataaaaata tgattttagt tctccatctt tgactactat tatctctcct
    22801 ggctctacat ctcttataac ttctccgcca agtatatcta ttgcacaatt ttctgatgca
    22861 aatatatatt catcaccctt tttaccaagt acaagaggtc tgaatccatg tgggtctcta
    22921 actgcaacca attcttcttg actcattatt acaagagcat atgcaccttt tatataatcc
    22981 attgtcactt ttaaactttc tactatatcc cctttgtaat atctagcaag tatatataaa
    23041 atcacttctg tatctgaatt agtttggaac atatatccat cttcttctag catatctttt
    23101 aaataatttg cattaactag atttccatta tgagctatgg ctaattgtct ttttctacaa
    23161 cttccaacta aaggttgaca gttagaaaca tgacttcctc cagctgttga atatctaaca
    23221 tgtccaatcc ctatatttcc ttttaactta gataattcat tttccttaaa aacatctcca
    23281 actaatccca tatctttttt gtacttaatt tctccatcat ctaaaagagc taaaccacag
    23341 ctttcttgcc ccctgtgttg catagaatat aaagaataat atagttcttt agttacatct
    23401 ttattagagt aaatccctaa aactccacac atacatttgc ctcctattta ttattcattc
    23461 ttgaaagtac ttccgtataa ccttctacaa ggtctccaag gtcttttcta aatctgtcct
    23521 tatctaactt ttcatttgta tttacatccc aaagtctaca agtgtctggt gatacttcat
    23581 ctgccaatat tatatttcct tcagaatctt taccaaattc tattttaaag tcaactaact
    23641 ttaggtttaa ttttaagaaa aatgctttta gtaactcgtt tatttttaaa gtttcttctc
    23701 ttaagaattt taactcttct ctagttgcta acttcattgc cactgcataa tcatcattta
    23761 gcattgggtc accataagca tcatttttgt agcttatttc aaaaattggc tcatcgaaaa
    23821 ctacaccttc ttcaagacca actcttttac agatagaacc agcagcaata tttcttacta
    23881 ttacttctaa tggtaaaatc tctacttttt taactaacat ttctctatct gataaacttt
    23941 ttataaagtg agtatttata ttattttctt ttaacatttc aaagattatt gttgatatct
    24001 tattatttag tatacctttt gaagatattt ctgctttttt ttctccatta aatgctgttg
    24061 catcatcttt ataataaaca acatattcat tttcattatc tgtagaatat acttgttttg
    24121 cttttccttc atataataac atttctgcta cccccttaaa ttcttgtcgt cttctaacac
    24181 cttttgtgcc atctcttctc tatatgattt caaatcctct tttaattttg ggtattttaa
    24241 agtcattatt tgtaatgcca ataaagctgc gtttaaacct aagtctattg taactgttgc
    24301 aactgggatt ccttttggca tttgaactat tgataaaagt gagtctagtc catccatagt
    24361 tgatgattta ataggtaatc ctattacagg aattaatgtt tgtgaagcta ttacacctgg
    24421 taagtgtgct gcttttcctg ctgctgctat tattacatca gtatcatctt ctatttcttt
    24481 taggaatatt gaaagttgtt ctggtgttct atgagctgat aatgctcttg ctacaacttc
    24541 tattccatat ttttcaagca gttttatccc ttcttctaat tttggataat ctgattttga
    24601 acccataact actgctactt tcattagact tcccctctcc gtttataagt tattttacac
    24661 tacaaatcat tcgtattatt atacgaatta tacaccttaa attttatatt aatatacata
    24721 tattatcaac tcttttttga aattttttaa aaaaaatatt tcttgtaatt ttttcattcg
    24781 ctatgtatcg aactttattt atgttttttt gttaaaataa aaaacagatt atctcaaaat
    24841 tggttttcta attaaatttt aagcccaata ctctttttta aatcttgtat attatttttt
    24901 attacttctt ttttaattaa tactttggat ttatagtctt gttttttagt tctgaatgtt
    24961 acttttactt agataaaacc aacacaaagt ttattcttta tataagtaat agctaaaatt
    25021 taaagtaatg atattatata aatagaaata attttattta aaggagaatc tgtatgttag
    25081 aagttataaa aaatagacat tcaataagaa catatataga taaaaatata gaagaagata
    25141 aaataactga aatactaaaa tcagctatgc aagcaccttc atcaaaaaat gctcaacctt
    25201 gggaatttat tatagtagat gataaggaac tgctgaaaca gctctcaaag tcacagcata
    25261 gagcaaagca tatagaattt gcaccattat gtatagtagt gcttggaaat agagataaat
    25321 tcttaaaacc tggtaaatgg atacaagatt taggtgcttg cacacaaaat ttattattag
    25381 aagttactaa tcaaggtcta gctgcttgtt gggcaggagt atttcctaaa aataaagtgg
    25441 taaataaagt tagacaaact ttagacttac cagtaaaatt agttccttat gcacttatat
    25501 ctattggata tagtgaagaa aaaaatgagt ttatagaccg atttgatgaa aataaaatac
    25561 atagaaatgt atataaaaat agataatgtt tagtaatata aatgaaaata catagtaaaa
    25621 atgcctttag aatacatttg tattctaaag gcattaactt tgattaaaca cctaattagc
    25681 ctctaaggta aaatttaact atcatttatt atttatatac caattgaata ttatatcctt
    25741 caatattttt atttatttca aatatattaa gaatatccta caattaaagt tagctatttt
    25801 tcattaaagt attttattga cacactcccc atagctaaag caaggggatt cttggtggct
    25861 aacacactta tttatactta aacagtacaa
//
LOCUS       DAELNY010000040        25679 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.040, whole genome shotgun sequence.
ACCESSION   DAELNY010000040 DAELNY010000000
VERSION     DAELNY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.040"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(27..1412)
                     /locus_tag="KPE55_001417"
     CDS             complement(27..1412)
                     /locus_tag="KPE55_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0492507.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(1585..2328)
                     /locus_tag="KPE55_001418"
     CDS             complement(1585..2328)
                     /locus_tag="KPE55_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG0492508.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(2348..3610)
                     /locus_tag="KPE55_001419"
     CDS             complement(2348..3610)
                     /locus_tag="KPE55_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0492509.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(3794..4792)
                     /locus_tag="KPE55_001420"
     CDS             complement(3794..4792)
                     /locus_tag="KPE55_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG0492510.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(4776..6287)
                     /locus_tag="KPE55_001421"
     CDS             complement(4776..6287)
                     /locus_tag="KPE55_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0492511.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(6341..7303)
                     /locus_tag="KPE55_001422"
     CDS             complement(6341..7303)
                     /locus_tag="KPE55_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0492512.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(7351..8241)
                     /gene="rbsK"
                     /locus_tag="KPE55_001423"
     CDS             complement(7351..8241)
                     /gene="rbsK"
                     /locus_tag="KPE55_001423"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG0492513.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(8283..9296)
                     /locus_tag="KPE55_001424"
     CDS             complement(8283..9296)
                     /locus_tag="KPE55_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0492514.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(9525..10502)
                     /gene="bioB"
                     /locus_tag="KPE55_001425"
     CDS             complement(9525..10502)
                     /gene="bioB"
                     /locus_tag="KPE55_001425"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG0492515.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
     gene            complement(10708..10971)
                     /locus_tag="KPE55_001426"
     CDS             complement(10708..10971)
                     /locus_tag="KPE55_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492516.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(11041..12114)
                     /locus_tag="KPE55_001427"
     CDS             complement(11041..12114)
                     /locus_tag="KPE55_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0492517.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(12373..13131)
                     /locus_tag="KPE55_001428"
     CDS             complement(12373..13131)
                     /locus_tag="KPE55_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492518.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(13128..14045)
                     /locus_tag="KPE55_001429"
     CDS             complement(13128..14045)
                     /locus_tag="KPE55_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492519.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDGGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(14045..14245)
                     /locus_tag="KPE55_001430"
     CDS             complement(14045..14245)
                     /locus_tag="KPE55_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG0492520.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(14250..15344)
                     /locus_tag="KPE55_001431"
     CDS             complement(14250..15344)
                     /locus_tag="KPE55_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492521.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(15854..15994)
                     /locus_tag="KPE55_001432"
     CDS             complement(15854..15994)
                     /locus_tag="KPE55_001432"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG0492522.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(16304..17611)
                     /locus_tag="KPE55_001433"
     CDS             complement(16304..17611)
                     /locus_tag="KPE55_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0492523.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(17676..18155)
                     /locus_tag="KPE55_001434"
     CDS             complement(17676..18155)
                     /locus_tag="KPE55_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG0492524.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(18202..19065)
                     /locus_tag="KPE55_001435"
     CDS             complement(18202..19065)
                     /locus_tag="KPE55_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0492525.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(19065..19820)
                     /locus_tag="KPE55_001436"
     CDS             complement(19065..19820)
                     /locus_tag="KPE55_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0492526.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(19847..20335)
                     /locus_tag="KPE55_001437"
     CDS             complement(19847..20335)
                     /locus_tag="KPE55_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0492527.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(20353..20754)
                     /locus_tag="KPE55_001438"
     CDS             complement(20353..20754)
                     /locus_tag="KPE55_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG0492528.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(20757..21254)
                     /locus_tag="KPE55_001439"
     CDS             complement(20757..21254)
                     /locus_tag="KPE55_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0492529.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(21278..21682)
                     /locus_tag="KPE55_001440"
     CDS             complement(21278..21682)
                     /locus_tag="KPE55_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0492530.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(21912..24479)
                     /locus_tag="KPE55_001441"
     CDS             complement(21912..24479)
                     /locus_tag="KPE55_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0492531.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            24645..25388
                     /locus_tag="KPE55_001442"
     CDS             24645..25388
                     /locus_tag="KPE55_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0492532.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNSNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         8042 a         4869 c         2667 g        10101 t
ORIGIN      
        1 tagttatgct tatatcaatt taagttttaa ataattggta tatatttaaa actacctaca
       61 gtatccttca attctttttt tgctttgttt ataaccaatt catcaagcat tacatcattt
      121 attgaacatg atatagattt agatgccaat aacattgctt taaatcccat atcagaacct
      181 gcactggcac aagattgcca tgtatgggca gaaataccaa taggccaaac tgaacaagaa
      241 cactgtgctg taggaattat gtagctaaca tctccaatat ctaatgataa tgatatacag
      301 ttattatgag taaatgtatc atccgtaact tcatcatgca gattcatctt agtaatatcc
      361 ttatttccac ttaatacact agaaacacta cttcttttat ctatagacac tgtctcagct
      421 aatttatctg caaaatagta gtcatcttca ttatagtttt gcacacctac taatttcata
      481 ttttgtgata gaatatctgt taatattgta tttggaatat agtcatatac tccacttata
      541 atgtcatatt ccataatagt accagtcatt aatgctgctc cttttgctat atcaacaatc
      601 ctaccaaata catctcttac atgctccatt ttaactcctc ttatgtaata ccacacttct
      661 gcaaaatcag gaactatatt tggcattttt ccaccattag tagttacata gtgtactcta
      721 caattatcaa atatatgttc tctcaaataa ttacaaccta tattcataag ttctactgca
      781 tcaagtgcac ttcttccgtt ataaggagct tgtgctgcat gggcactaat cccattatat
      841 ctaaatttca cagagtaatt agcaagtgtt ggtattctta ttggagtatt tatatcaaat
      901 ggatgccaac taaaagcaca atcaattcca tcaaaaaaac ctttttttat catcaatact
      961 tttccagata aatcttcttc tgctggacat ccatagtatt ttatagtccc tttcagcttt
     1021 tttagtttga ttaattcctt tactgcaatt acagctccta ttgaacctgt accaagtaag
     1081 ttatgtccac atccatgacc tgcaccacca tctacaatag gttctttagt agtggatact
     1141 ttttgagaca aaccatctaa agcatcatat tctcccaaaa ctgctataac tggtctacct
     1201 tctccataac tagccacaaa tgctgtatcc ataccttcta atccctctat actaaaacca
     1261 tgttttttta aacattcttt ttgtaaattt gaagcatagt attctttata ctgaagttct
     1321 ggatttttcc atatcttatc tgatatatct cttaattctc cctgtatctc atctatatat
     1381 tccatcaaaa actttttatt taaattatcc atattaatta tataaacctc cctgtttcta
     1441 ataacttatc gattatcagc caaaatgact aattttaact gatttaaaaa tattttctac
     1501 catacaacca ttttatacaa taaaacctct tgacttgtaa gtttcatttt atcaagtaag
     1561 aggttttcaa gttttataaa atttttagca tgttaaccaa tcaagcatat taacccataa
     1621 tttattatag tatttccaat cagtaaattc ttttggaccc cagtgaggtg cacagtcaga
     1681 actaaagata gcagattttc cttttccaaa ctcgccaaca gctacaaatg ggtctcctcc
     1741 aatagttgct aaaacaggac aattatctct agctactgtt ttgttatatc ctaaaaactt
     1801 tggccattct gttggtatac cttttaatac tggatgttca gagtcaataa cttctggaat
     1861 tattcctgct ggtttttcaa ctctatcatc tttatctatc attgttattg gtaatacatc
     1921 tttgatagca gtttctccaa atctagcttt tgcatctatt cctgtaaatg acatatatcc
     1981 acctaccatg attaacgccc caccattatt tacatactct ttgattagtt caagtctatc
     2041 tggattacta ttgcaatcta taaaagtact attagataat aagaacgtat tagaacctat
     2101 atcagataat attatacagt catactcatt taactcttct aatttatacg gaaaactatc
     2161 tgctgctaca tgtgcaggca tatgaactgt ctcatatcct ccagattcta ttgcttcttt
     2221 caaccacttt acagcttctt cataatgagt tgtaacaaac gtatcaaacc cttttatatg
     2281 agttatattt tttacccaag attcccctac tattaatact tttttcattt atatattcct
     2341 cctactttta taagtttaat attagacttg catacataag tatactatct aaatactcct
     2401 taatttcaac atattcatca attgaatgac attgttctat tgaacctggc cctaatataa
     2461 gtgttggtat ttcagctaaa tttttcatta ctctcgcatc attgccagct gttgaacctt
     2521 ttatctctaa tttttttcct agagtttctt ctagtatttt atgtgcattc actacaaaat
     2581 cagaatcctt gtccatctca aaacctaacc ctgattgata tatgtttata tttggtttat
     2641 tatctttaag ccataaatct ccattagccc ttgtcattat gacatttgtt acatcattta
     2701 caacttgttc ataactcatt atatttggca agaagtgtac acataaatta aatacacact
     2761 tatctggcac agtagaacct gctgttccac catttattac acctaaattt attgtaggtg
     2821 aaggtaatag tggatgttta tatatcataa gccagttatg ttccaactct tttatatctt
     2881 gaagcaacaa catagccttc tcaattgcat ttacaccctc ccacttacta ccacagtgaa
     2941 gagacacacc tttaacctca acttcaaaga atacaaatcc catatgtgca actattaagt
     3001 tttgttctga tggttcacaa attatgcaac aatctccatc aataccattc ataactgcat
     3061 ttatggtacc atttccgcca ccttcttcat ctactactga cattattttt acattaccag
     3121 gtacatttag accactatct tttataagct ttacagctaa tattgatgct ataagtccag
     3181 atttcatatc agcagttcca agaccataaa gttttccatt atcttctgta gcacgatatg
     3241 gattatattt ccacttactt atatcacccg gtggcatagt gtctacatga ccattaaata
     3301 caattgtctt accaggtaaa tcatcactat acttacaaat taaattatat ctatcttgat
     3361 aattgtgacc tagatttcct tctttatata tattttttgc ttttttaatt aattcttcgc
     3421 tcatttcttg tctatccaca ctaaaaccta tgtaatttgc aagtttttca atatattcct
     3481 gtccttcttt ttcaaatcca cctagtattc catgaccaac atcttcagtc tttatactta
     3541 caagttcttt taaataatct atgtattctt gttttttact atttaatgta tctaagagaa
     3601 tttttttcat atttcctcct aagatttatt tttacattta aaatttcaag tctaatctca
     3661 tctatttaag ccatagtaaa ctttttcact tagcatattc tcattcaatg tataaagatg
     3721 tatgcaaatt aaagtataaa aatatataca aaaacttaat aatttttata gtttataagc
     3781 taaaatttaa ttactattta gatttagctg atttttttaa ttttatatta tttatccata
     3841 tagcaaataa cattaccaaa ccttttataa tcatttgatt atatgaagat acacttaata
     3901 agtttaatcc atttcctaat attcctatta ttccgacacc tatacatgta cctactatag
     3961 aaccagaacc acccatcata ttagtaccac caagcacaac ggctgctata gcatccaatt
     4021 ctgctccatc tccaacagtt ggtgaacctg atgccaaacg tgatgatagt attatcccag
     4081 ctaatgctgt tagtagtcca gctagtgcgt atacttttac tataacttta tttactttta
     4141 ctccacataa tctagcggcc tcttcatttc cacctactgc atgtacatga cgtccaaaat
     4201 tagttctttt taatagaaaa tgtcctgcta ttacaactat aatcataatt ataactggta
     4261 ttggtattgg tccaatatat cctctaccga gcatcttaaa tgcatctgta tttactgata
     4321 taggtcttcc tcctgtaaat acaaagcttg ccccacttat tatcgttaac atggctaaag
     4381 ttacaatgaa tggaggtagt ttttgtgttg aaataattgc accttttatg actcctaata
     4441 ttgtccctat tgctaatgca agtatcatac ctacccatat aggtgcatta taattcacta
     4501 ctgttgtagc ccaaaatgta cctactaaag caattactgc tcctacactt aaatctattc
     4561 ctcctgtaat tattacaaat gtcataccta ctgcaagtat tgcatttatt gatatttgtc
     4621 ttccaacatt aattaaatta tctaaagtta aaaagttaga tgaagctaca ctcattatta
     4681 taaaaattat tacaagtcct acagctgcac tcccttggtc tctaaataca cttgttaatt
     4741 ttgtcaataa tggagaagat ttttcttctt tatgcttagt tattgaactc attaatttta
     4801 cctccgaaag ccaattttaa tacattattt tcaaccattt cttcaccttc taaaaatcca
     4861 cttatatgac cttctctcat aaccattatt ctgtcactca ttgatattaa ttctggtatt
     4921 tcagaagata tcattattat acttacacca ctctccacta aatctcccat tagattgtat
     4981 atctctgcct tagcatttac atctatacct ctggttggtt catccataat caatatatct
     5041 ggttgtaata aaagccatct tgctattata gctttttgtt ggtttccacc acttaaactt
     5101 tgtataactt gattcatatg tggtgttttt atctttaaag cttctatgta tttttctaaa
     5161 atctcttttt ccttttttct atctataatt gatttaaact tagatatctt ttttaaccca
     5221 gccatagttg tattttcttt gacactcatt tgtaaaaaca atccagtacc ttttctatct
     5281 tctgttatta atcctagtcc attttctatt gcatctgatg gagttttaaa ctttacttct
     5341 tcattgttta aatatatttt tccagaatca tatgttctaa gtccaaatat agcttctact
     5401 atttcacttc tcttacttcc aactagtcca cctattccca atatttcacc ttttcttata
     5461 ttaaagctaa tatcattaaa aacattgttc ttagttagat tttccactcg cataatttct
     5521 tcttttattt ctacttttct ttttgggaaa aattggtcta gctttctatc tatcattaaa
     5581 ttaactaatt cttcctcaga cgatacatct ttggtattta aagtcgaaat cgttttccca
     5641 tctcgcatta tagttattct gtcacaaatc tcaaagagtt cttccatcct atgggatata
     5701 tatattatac taactccact ctcttttaaa tcatttataa gtctaaaaag tatttcagtc
     5761 tcttttttac ttattgaaga agtaggttca tccattatta atactttagt ttctgatgat
     5821 aaaactcttg ctatttctac catttgtttt tgagctatac taagctcttt gactaaacta
     5881 tttggattta tatctattcc catattatcg aaaatctttt tagcctcttc attcattttt
     5941 ttgtcattgg ttaagacttt gccatttatt tcttttccca taaaaatatt ttgtgctact
     6001 gttaattcgt caaaaactgt tagttcttga tatatggcat ttatacctat ctttttagag
     6061 atatctgggg atatattctc tatttctttg ccttcaaaaa taaattttcc agaagttttc
     6121 gaatgtgcac cagttaaaat ctttatcatt gttgatttac cagcaccatt ttcacccagt
     6181 agtgcatgaa cttcgccatg aaataactca aaattaacat tatctaatgc tctgacacca
     6241 ggaaattctt tagcaatatt acttaacttc agaattatat tactcataat gacactcctt
     6301 tttaaatagg atttctttca aataatctca ttttatttta ctaattctta aagtctttta
     6361 tattttcctt atcataaagt aaagctggta ctggtatatt cttttctacc ttttcccctt
     6421 ttatagcttt tactactgct tctacagcta aacgtcccat ctctttaggt tgttgttgaa
     6481 tcatagcaag aatagttcca tctgttattc cacttgatgc ttcttcagtt aagtcaaatc
     6541 ctacaatctt aacatcctta ttttttgcag attcaagtgc agctttagct cctagtacac
     6601 tattttcagc tgttgcatat attatatcta catctggatt agattgaatt aagttttcta
     6661 ctgtattaag agattcttct ctgtcatatc ctggttgtga atttaatatc ttaacatttg
     6721 ctgaaccttt taatgcatct ttaaatccat ctattctctg catttgtatt tgagatttta
     6781 aatctgtcac tattgctatt tctgactttc cacctaactt ttcatctatg tattttttag
     6841 tgtattcccc taatttttta ccagcatcat aactaactgt tcctacataa cttgtaatat
     6901 tttcagtatt taatacagca tctactgtaa ctactggcat tttttcaaat gctccttcta
     6961 ctgctggtac tatagccgaa ttatcagttc caagtactat cattccatct acattttgtt
     7021 gtataaaatc ttctattgct gatgtttgct tagctacatc ttggtctggg tcatttacta
     7081 taactttcac acctaattct ttagcttttt cttttattcc ctcatcaata tctataaaaa
     7141 attgatgttg aagagttatt gtagatactc caatagttaa atctttcttg tctgaaccac
     7201 ctttattatc tgatttactt ggtccttcca ttgaacaccc tgttaacatt cctattgcta
     7261 gtattccagc aattcctaaa gataaaattt ttctcactct cataattaac ctccattttt
     7321 aatagtttta aatttgaaca aattagattt ttatttatta tttaaatact tttcaacatc
     7381 ttctttatag ggcataccag attgtgcacc tagtttagtt acagaaagac ctgatactac
     7441 tactgcatat tctactgctg tatataaatt tttcccttct gttatagcca ctgccaatgc
     7501 tccattaaat gtatctcctg ctcctgtggt atcttgaaca tcaacactta tacaagatac
     7561 atgcttgatt tcctcaccat cataaaatct agctccattt tttccttctg ttattacaag
     7621 tttatttgga tattttttta atacttcttc tatcccttca tttgtgtcaa aaactatctt
     7681 atattcatgt tcatttggtg ttaagtaagt tactttttct attatatctt catttagttt
     7741 tactgctggt gctggattta ataaaaccct aatcctattt tcaaaacaga aattcactac
     7801 ataatttatt gtttttaatg gaatctctaa ttgtagcaat actatatctg cattctttat
     7861 ctcttcttca ttctttttaa ttaattctat atctactagc tcattagtcc ctggtacaac
     7921 aacaatacta ttatcatttt cacataatgt tataacagca acaccagaac ttttgtgatt
     7981 atttctggat actaagctat aatcaacttt attcgttcta aaatttctta ttaactcctc
     8041 tcctaagcca tcttccccta tgcatgatat catttttaca tttgctccta gtttagaagc
     8101 tgagatagct tgattagccc cttttcctcc aggtgatgta aaaaaactat tacctaatac
     8161 tgtttctcct ttttctggtc ttttatctac agaacatacc atgtccatgt ttacacttcc
     8221 tatgacaact atgtttccca ttacattact cctttacata tctatttatg tctctcgtgc
     8281 tgttattcac tttcttttgt tgaatctctt atgaccaatt ctggtttgaa aatcaattca
     8341 tcaaacgatt cttttttatc tcctcttaaa caatttatca atatttcact ggctttatat
     8401 cctatatcat aagacggctg ttttactgtg gtaagagaag ggcttattag acttgatgtt
     8461 tgaatatcat caaatcccac gacacttata tcatttggta tacttaatcc tctttcttta
     8521 agacctctta tgacacctat tgcaatcaaa tcatttgcac aaaatattgc atcaaaatct
     8581 atcttttcta aattttttat aaattcttgt ccccattcaa tcttatattt tccttcaaat
     8641 acataagcag gattgtagct taaattgcac tcgattagag cctttttata tccttctaat
     8701 ctatctactg ctatttcatt ttgaagtgga ccacttaggt atattatttt cttatttcca
     8761 atatctaata aatgttttgt agctatatag gccccttcaa agttatttat tttaacaatt
     8821 ccttttaagg tttctttgtt catacttatc ttcttatcaa tcaagacagt tggtattttt
     8881 atatctttat aatttgcttc tttccagttt gtatttgaag atgctgcaaa tataattcca
     8941 tctatcatcc tctctgatag tgttcttatt ccttcatatt cttttttagc atcttcatct
     9001 gtattacata aaattatatt gtagccttct tcattagctt tatcttctac acctcttgat
     9061 acatctgtaa agaatggatt tcgtatatct ggtataagaa gccctatagt attagttttt
     9121 ttagttacta aactttgtgc taatttattt ggtacataat ttttttcctt cattattttc
     9181 aaaatttttt ctcttgttgc ctgacttata ttttcatctt tattattaat gacttttgac
     9241 acagttgttt tagaaacacc agccaattcg ccaatttctt ttatagttat actcaagaag
     9301 ttcaccccca aaatttagga aaccgtttta cttaaccggt tacctagata ctatcacatc
     9361 tttttttcat ttgcaaccat tttatataaa ttttgatatt tttccctttt ttcaaaatat
     9421 tgcaacttat aattcaattt taatatttaa attaatctgc aaaaaaaaat taatatactt
     9481 ttataattat ctaaaagtat attaattaat ttaagcagta tttattaaaa ttcaaatcct
     9541 tgctctataa tcattttttt atcattttca atcccacttc caacagttgt caaataatca
     9601 ccaacaattg cagaatttac tcccccatta aatccaactt tgtcatatcc ctttaatgat
     9661 agccttccac cagcatacct tatgtacaca ttaggaatta taaacctaaa tactgctgtt
     9721 gtttttagta tttccattgg ttctaataca tcataatttt ccattggtgt attcttaata
     9781 ggatttaaaa tgtttattgg aaatgatttt acatttaatt ctcgtatttc aaaagccatt
     9841 tttagtctct gttcttcact ctcattcatt ccaattatac cgcctacaca tacatctagc
     9901 ccagcttttt tagcatcttt tatagttttt atcctatctt tatatgtgtg tgttgtacat
     9961 atattatgaa aattatctct acacgtctca acattgtggt gatatgtagt cacacctgct
    10021 gacttcaaca ttttagcttg ctcataatct ataataccta aagatgcaca taattttaaa
    10081 ttagtatttt ccctcaatcc ttcatagata tttaaaatat tattaaactc ctttcctgac
    10141 atacctttac cacttgtaac caatgagaat ctatgcactc ctttactctc catctcttta
    10201 gctctattta agatttcatt gtaatctaat aatgaatatt cttctacacc agttttataa
    10261 taagaagact gagcacaaaa tttacagtct tcactacatt tgccagattt agcattcatt
    10321 atcgtacata aatctgcttt tcttcccata aaatattctc ttatttcatt tgcagatttt
    10381 aatagaatat caaaatcgtt tttattattt gtatcaaggc ttattaaatt aagagcttct
    10441 tcataactta tttctttttc tctcaatact ttatttttta attttattat gtatttttcc
    10501 atatatcctc ctatttgtca acttaatatt tttatgtagt ttacttatta tatatagtat
    10561 tgcatatata acatttattg gtcaacttta ttttagacac aagtttacta tacacaaaaa
    10621 aatactgctt caaaacaata ttagtatata gattatacat attattatta aagcagtatt
    10681 ttttattaga atataattct tactggttta taaaactatc cttgtcttaa ctccatccaa
    10741 gctttccatt gtaataactt ctaagccttc atattctcta aatagccatt ttaaatcctt
    10801 catggcttga ttaacctgac tttttgttag attatttata aaatattctt ttctagtttc
    10861 ctcatactct ttgctagtca aaaagtttct tagaaaatta aacgcaaata cacttggaat
    10921 attgtttaca ttagccttct tgctgttttc cttctcatag taaattttca taaatgcata
    10981 ctcctttgat acttttagat ttctcttaca ataattatat gcatttaagt gctactattc
    11041 ttataatttt attaattcat attctttact tcccataccc atttcacatg catagtctat
    11101 tattctagtt ccatttacat gaggatgttc atgtttgaaa acatcatgac ccatttcctt
    11161 acaaatcaaa tcatagcatg cttgttcaat agctacaggg tctgttgatg ctaatattcc
    11221 tatatcatgg gcaattggtc tacctgacca aggtacacaa tcacaaagtg gagttacatt
    11281 cataacaaaa gttatatacc ctaccttgtc ttttttattt gatactgctc cataagcgta
    11341 ttctgccatc ttttcgacaa acacatctga gtcactttcc cattgtatag ttactgctct
    11401 agttggacag acagttggac attctccaca tccataacat acatcagaat ctataactgc
    11461 ttttttatcc actatactta tagcttttgt aggacaagaa ttaacacact ttccacaacc
    11521 tacacacttc ttttccttta ctactggagt aacatcagaa tgttgcattt gttttcctgc
    11581 tgcacttgca catcccatag ctaagttttt taatgctcct ccaaaacctg ctgcttcatg
    11641 acctttaaaa tgactcatta ctatcattgc agaagaatta taaatttctc cacctatttt
    11701 tacactttca aaatgttttt tgtctatttt tacattctca taatttctac tatataatcc
    11761 atctgcaatt attactggtg catttacaac tgcataagca aatccatttt ctatagcagt
    11821 tgtaagatgg tctacagaat ttgttctact ccctgtatac agtgtatttg tatctgttaa
    11881 aaatggcttt ccttcgcagt ctttaacctt atcaaccact tgtctaactg ccacaggact
    11941 catatatgta gtatttccct tttctccaaa gtgaagttta attgcgactt ggtcattttt
    12001 ttctattaaa tctttgaatc ctgcttcatc aaataatctt cttacattgt ttggtacgtt
    12061 tttattttgc gaatttgagt ataaatcaca aaaatatact tttgactttt ccatatctac
    12121 acccctttaa aatatttact tatactaatt ttatcacttt taaagtaatt tttagtatat
    12181 tttaacataa ttaaatattc ttatatttat ttgatacaaa taatattatt tgtattattt
    12241 tctacactta attctatata ttaaactaca cctaaaaatc aaaaagactt gaaacttaag
    12301 tccaagtctt tttgattttt aggtgtagtt tttaacaaca taagattact ttaccttgtg
    12361 ataaaggtat tattaaactg gttttttatt aaataaaata acagctgcta gtaaacatac
    12421 tatagaaata agaactgtta taataattgg aataataaat tctggcattg ccactttccc
    12481 tgaaatcaag tctatatttt ttgacatcaa gaaattagga gtataaggtg caatctgttt
    12541 aggaatagaa attaaactca ataagagagt tgctaccaat acaagtaaaa cactggcaaa
    12601 tgctggtcta aatagtacac acccaagaat taatacacta atatacataa ttcctataag
    12661 ccataaatta aatgctgaaa atataatatg attaagattt gcagttggcc aaaaatacat
    12721 tgtatagcca taagcaaata aaaaactcaa ccaaaaacca attgtcataa taatcaccgt
    12781 aacagagaat ttagacaata ctatagatga acgtgataaa cctttagtca gcataataac
    12841 aagtgtccct ttagaatatt catttgataa aatattacaa aaaataatta gagttaaact
    12901 cataccaaga ccagagatat tgctaaaaaa ttgtgtccaa gaatctaaag cagttggtgc
    12961 agcagtaact tttaaagtcg gtgcaaaatg agcaatcaag tctggcataa attttgcaga
    13021 aataggacta cttataccaa atattagaaa taaaataatt aaactaaata atttataatt
    13081 tcttaaattc tctttaaatt ctttttttgt aaatgctatg tattctctca ctttactacc
    13141 tccaaaaata aattttcaag tgataattct attagttcca tctttactgg acaaagattc
    13201 atttctgcga gtgtagatat aactgttttt tgtattgctt ttatatcttt accatgcaaa
    13261 acaatttcca tattagtttc ttctgaatct tttagtaagg atttaatacc atcactagac
    13321 ttaaactttt gaatttcatc attacttgca aactccagta aaagtctatc ctttccgtgc
    13381 atacttttta tttctgaaag tgtaccacta agggcaatgc ttcctttgtt taatatagca
    13441 acatggtcac agattctttc aacatctgat agtatatgtg ttgaaaatat tacagttgtt
    13501 gagtctttga ttttaagcat aatatctaat atttcttttc gaccaacagg gtcaagtgca
    13561 cttgttggtt catcacaaat aagtaatttg gggcgtgaaa gcaaagcttg agctatacca
    13621 agtcgttgct tcattccacg agaaaaccca cctattcgtc tcttttcatt tccaagaccc
    13681 acaagtgata acaactcttc actccttatc tgtatttctt tttttgaaag tcccgtaatt
    13741 tcaccacaaa gagaaagata ttcaagtggt ctcatatagt tgtaaaactc tggaacatca
    13801 ggcagataac ctacatgacg attagaactt gttttcccat aggtcacttt ctcaccacaa
    13861 acatctatag ttccaccatc tggtttaagt aacccaagta ccattttcat tgttgtagtc
    13921 ttacctgcac cattcttacc tacaaaccca aagactgacc cttctggtac agtaaaattt
    13981 aaattatcta ttatctttcg attaccaaaa ccttttgata atccatttat tgagacaata
    14041 ttcatcattc ttcacctctt cctattgtaa aatacaaaat tggtcctatt agactaataa
    14101 acagtactac aataatccat attgccttat ttccaatttt ataatttgga tgtctcaata
    14161 catgaacaag tgcagtaatt ataagtatta aatctaaaat aataacagga accaaaagtg
    14221 gtaagtattc catcaaattt tccatactct tattcctcca ataaagataa tttttctata
    14281 agaaatttgt attctttgtt ttcacttaat acagaaaaca ttggattatt tttaatagca
    14341 gcaacaatac ctgcttttac gattgtatca tcacgtgtaa tacctgttcc aatttcagaa
    14401 atccatctgt caagtttatt aaaaaagtca tccccatgta ttataattgg gtatatatca
    14461 cgtgttgcta aatctacata ttttcgtaat gatttaatag ctccttcatt atcaccatgt
    14521 attaaattaa tttgtgcctg tgttaaatat gaagaaagca taatagcagg agataatgta
    14581 tcaagctcaa atatatctga aattgctaga atacgtcttt ctatttcctt catttgtaat
    14641 ttatctgcat tttgcaaaat acccactaga ttttggacta atgatataag gttctgatat
    14701 gcaccaagtt gaaatgtttc tcttgcttca tccatttgac cattcatcat ttgcccttgt
    14761 gcaagaagta tagattcatt tatcatggga aaattgctgt tttgcaataa atcaatcact
    14821 tgtatgggtt gatttaacaa tatataacaa gttgcctcca tactttttgc ttgacggcaa
    14881 atatctatgt cattacaagt ttcctgtata cgaataaata tttctaatgc ttcactaatt
    14941 aatagttcac gctttttttc atctacaata tcatagtgat taactattaa aagacccatc
    15001 cgaaaaatca aaggaaaaca tgaataatat ttttttatga tttctcttat ttcatccatg
    15061 acttcatcaa atggtttaac cgtaaagtct ttgcacagtt tattatacag tttattaata
    15121 tcttccttca tcatttgagg ttcataacat attaactcat caacggtaat gttgaaatat
    15181 gtcgcgagtt gcggtaaaaa ggttatgtca ggatagcttt gtccagtttc ccatttagat
    15241 acagaagctt tagaaacgcc tatataattt gcaagttcat cttgtgtaat tcctttttct
    15301 ttacgcttta aaatcagcgt ttttgcgatg tttaattcat tcatgtcaat ccctccttat
    15361 gtataattat atccttaaac atgtaaagct aacaagcgat ttgaagttga cttttgacga
    15421 aaaaatcaac cacagagaaa caaataaaca agtatataaa atatatattt aaatttatct
    15481 ttaatctcct ttgtaaaata tgtaaacttt gtaaataaaa tccatattat ataaaactaa
    15541 tagttaatct atctaaatac agtttttatt tatcacgttt ttaaatattt tgtgtaaaac
    15601 ctcttttaat ataaacacct tattttcatt tctcatttca tattcttttt aaaatactga
    15661 atataactct tcgattttcc atttaatcct tttataaagt tattattcgt atttattcct
    15721 ctaattataa caggcaacta tcatgtattg gtatttgtct attatataaa aatctataaa
    15781 agaaaagcag tcaatcattt agattaactg cttgtacata tatcttatta aactgtataa
    15841 actataattt ttattattct ccattttcat cccaagtcat aaatccccat ccactatctg
    15901 gataaatatc gatagacttt tctctaatca tataaccaac tttataacac tcttttgtta
    15961 ttttattaga tagtatacat ccttctccat ttatctaatt ccatttaatt aacttttctt
    16021 ttcaacctta acttttataa aatcatcttt tttaataatt tcatacttat tttctcttca
    16081 acatcatcaa atgagggtgt ctcaaaatta actttttact tcataaggca ctcttttttg
    16141 tatgaagtaa aatatatttt catcatttca acaataagta agaggcttat ctctattttg
    16201 agacagcccc atttgatggt gttgaacatt taaataaggt tgaactcgtt ttatctctct
    16261 ttttaagata tctgtaaatt tgcacataaa gtctcttctt cttttacaat tctaatagtt
    16321 ttattaaagc caaagtatat atctccatat tctttcgtat atgatttatt ggtactcttt
    16381 catctactcc atgaccacca gttttatacg ctaagaactt aggtccaaac gccaccatat
    16441 ctttaaacca acttgcatat gtgccaccac ccatagcaat tgtatatcca ttttcatctg
    16501 ttacttcttc ataggcttct aataattttt taagtactaa attactctca tccacataat
    16561 gtaagtcttt atgagcaata acctctattt tccaaccatt acaaaaaacc tttttgaatc
    16621 tatctattat attgtttaaa actatatttt taccatacct tatatctaac tcacatacga
    16681 ttttttcctc atttactttt aatattccta aatttaaggt agtgtttttg aatacctcat
    16741 catattgatt aatccccaat gcatctccat aataatcact acaaaggtat tttgaaacta
    16801 attctatatc ttctctatgc tgaaaatcca tattatcctc aactatcttt attaccattt
    16861 tagatatagc attttctccc ttttcaggat tacttgcatg tacagctact cctttagtag
    16921 ttaactctga aataattcca ttaataattt ttgcagtaca atgagatgga acaacatttt
    16981 ctttagttcc tccacttact attatgtaag gtttactttt gtcaatttta atttcttttt
    17041 ctatccttat atgaaccctt cctttttctc cattaaccac tggatacata gcatctggag
    17101 taaatcccat acttggaatt tcttcacctt tatctaagta atatttgata catttcatac
    17161 ctgtctcctc atttgtacca aaaattaatc ttattctttt attaattgta ggatacatat
    17221 cctctatgtt tttcaataca tgtataagca tagatacagc acccttatta tctatgacac
    17281 ctcttccata aataatattg tcaattactg ctcccgaaaa tgggtctaca ctccacccct
    17341 ctcctggtgg aactatatct aaatgcattg gaatccctat taattcctct ccctctccaa
    17401 tttctgcata tccaatataa ttatcaatat ttttaacact aaaacctaaa ctagcacagt
    17461 aatctaaagt tttatctaaa gattttttag gtcctatacc aaaaggacat tgtgaaatag
    17521 gttcaccttg ttgtgaatca atttttatca attcttttag aaattctatg gttctattta
    17581 aactattatc tacacttctg cttatatcca taaatcaacc tactttctat ctttttaaag
    17641 cctgtccttg aagaacaggc ttttagcatt aaaaattatt tttttgtaaa tactaattca
    17701 caaacttcat catcatgagc acatttttga gcaaaatcta atttaacacc ttgtgcacag
    17761 tcaactcttc cataatcccc ataacaagct aatctacaaa tttcttttct ttcttcttta
    17821 gttaatccat attctttcca agcagcatct aatggacagt gatggaattt taaaacgcca
    17881 ttctcatctg tattttctac taattccatt tcaaacactt tttgtccttt actgttatat
    17941 aacatttctg ccatttttcc tggagtatct gctacgttat atttttgacc tctcatttta
    18001 ccaaatccat atattgcttc tttacatata tcatctggat tagctcctaa tttttcagct
    18061 tctttcataa gaagatagaa ccatactgct ctttctcctg ttgctcctct aattccttct
    18121 aaatattcct tttctgtata cattttttct gacatttctt tctcctccta aaattaaatt
    18181 ctatatttta atcactgtga attagaatat tccaatgaat gaccctacca atgctaatac
    18241 aactaaaatt actaataact tagttattgt ccacttcttg tatttcattc cccaatatac
    18301 gaagaataca acaccaagtg gtaataaacc tggagcaatc atatttaatg tatcttgaac
    18361 aacaattgct ttaccaccaa tatcaaatga tagcggagtt gaaagcttaa ctgtagttgc
    18421 agataatgct cccatcataa acattcctag aattgaagca ccatcaatta tcttattgac
    18481 aataccacct gatagaattt tcttaattga atctctacct aaagaatatc ctaaatgaaa
    18541 taagtaataa ccttcgcaaa atagtaatat actaaacatt attgggaaaa atgcacctat
    18601 aacacttcct tctgctccaa atgaagcacc taaagctaag aatattgttt gaaatgtacc
    18661 aaaatctaaa gtatctccaa ttccagccaa aggtcccata agaccattct taacacctga
    18721 aataacttct cctgggattt ttccttcagt agctttctct tcttccatag ctaaaactgt
    18781 accgtgaatt aatcctcccc atatagcttg agtattaaaa aattgtaagt gtctctttaa
    18841 cgcatctact aaatcttctt ttctcttata taacttttct aaagccggag tgaaacttgc
    18901 acaaactgct aatgcttgca ttctttcaaa agagttggat aattctgctg tccaccacca
    18961 tcttagatat gtttttgtta catctttctt tgtaagtact cttgtcttat taacttcagt
    19021 tggaccttgt ccatttttaa cttctttatt ttttatttct tgcattatac ttcacctacc
    19081 cttttgtctt ctttcataaa ggtaattaaa agagctatac aaacaccaaa tattgcagct
    19141 gcagtaattg atatttcaaa atattgaacc ataaaaaatc ctataataaa cattggtaaa
    19201 aatttatttt tacctatagt aaatattgtt gttgcaaaac ctaatgcagg taatataccg
    19261 cccattactg ttaatccatt cattaaccat tgtggtataa catctaataa tgattgaact
    19321 aaatctgctc caaaaaagtt acatacaaaa actattggaa accttaaaac aaacccaaat
    19381 attaaagaat aaagtgttgc acatctccag atgccttttg tattaccttc ttcagcatac
    19441 ttatctgcct tatgaaccaa tacagcatta ccagttcttc ttaagttatt aactaatact
    19501 cctataacac ctaaaggtac cgcaatagat actgcaactt ctgcattaac accagtttga
    19561 agtgctactg gtattgcaat aagagcagct agacatttat ctgatggaat atttccccct
    19621 gctgcgacca ttcctagata aaccatttct atacttgcac caacaatcat accagttgtt
    19681 acatcacccc aaagtaatcc aaggctaaaa cctataaata aaggagactt tagtgttgag
    19741 aaaagggtat accctgcaag acctgctgca aaccaatacc ataatcctgt tacaattgcc
    19801 aacatcaaaa cagattccat ttataattcc tcctaattta tattttttat ttaaatttct
    19861 ttaacgcatt ttcatatgtc atagatgatt cctctggtat aacttgtagg actatttcaa
    19921 ccccattttc attaagttca ttaagttgtt ctacatcctc atttcctaat gaaacagctg
    19981 taaacaccat tttctttcct gcttcatttg gaatacctcc taattgaatc ttttcatatt
    20041 taagtccagc tttataagcc ttatttgcca tatcaacatt tttaaataga ataaatatat
    20101 ttttatctcc taaagtgttg ttttggaatg cttgtcccgc atcttctgct gttaaaataa
    20161 ctacttctac tcctgatggt gctgccattt tatatatgtc agccataaat tcatcttgac
    20221 ctaagaagtc atcaactatt attatttttt gagcttgagc gatttttacc cattttgtta
    20281 taacttgccc atgaattaat ctgtggtcta ctcttactaa tgttattttt gccattatat
    20341 tcacctcata atttatattt ttttaaattc tttaattaaa tccacacaaa ggttattatt
    20401 ttttttgacg attctttctg gtaatgtatg taatttatct tctttgctta attcttcatc
    20461 tacgattaaa attgtctgta agctaagtcc atttattgca tctattcctc ttcttatagc
    20521 aacagctatt gcaacacttg atgtagatcc tccatataaa tctgatatta taattttcac
    20581 atcttcttta tctactactt cttcaacctc tttcataaat acagaaacat ctttttcaga
    20641 acttagtgat aaaacttcat attgatttga caatccaaag tttttatttg tttcttctac
    20701 taagactttt ccccaagttc catggcacag tatgattacc ttatatttat tcattgctaa
    20761 ataaactctc ctttatagcg attaatattt tggtttaatt ctacttcttc ttcatcaggt
    20821 accatttgaa ttgatacatt aatattcttt ttaataaatt catctattaa aagaaactct
    20881 tttttattaa tgtatacatt ttgagatatt ttcttttttc ctattcctgt aggtattctt
    20941 ccaacaataa gttttttgat attaatccct tcttctatca ttctatttgc agtatctaaa
    21001 tttttaatta atacaaaaac gttatcatca gcttgattat tataaaaata tctccttgat
    21061 tcacttacag ttaatattga taaatcaata ttctttggtg ctgccatttt tatcaaacct
    21121 ttagcaataa catcattggt tgtttcatca tctactacta ctatcttttt tacacctaga
    21181 tgcttaaccc atttatatat aacttgacca tgtattaatc tatcatcaca tctgcaatat
    21241 tttactaaag acatttttaa tcaaactcct ttttctatta cattgagcta agaactttat
    21301 taatattgtt tataccagtt tgtcctgctt caatacatgc ttctgctaac tcttctaaac
    21361 tttttccctc tctattaatt aaagcttcaa gtagcattgg aagatttact ccagtaagtg
    21421 catgaaactt gccacctttt ttaaggttta aacttgcaac attacttggc gaacctccta
    21481 ataggtcagc taatactaaa actccgtcac cttcatctaa ttcatttaac ttttctacaa
    21541 aatttttatt taagtcttca atattatcat tcctatttag tttaattgct gctgttcttt
    21601 ctgctggtcc tgcaataagt tcacagcttt ttataatttc ttcactaaaa ctcccatgag
    21661 tcataactat tattcctatc accaatttca cctcactttt cttgtcactt tttatataag
    21721 caattgccgt gccaacttat gtgtatcaat aacaaatggc tattttacag gatttaatca
    21781 aaacttttta ctcaattttt tttaaaaact tattatttcg atatgtatca aaagattttt
    21841 tcaacctgta tcaaatctgt atcaaaaaaa gtaaaaaaaa gatagccttt tggctatttt
    21901 taaattttgt atcaactttt gaatttatct gtatcatatt gtgattcaaa tatatctaat
    21961 atatttgcat attccatatc tgaaataatt atatcgaatg tctcttctac tagcttaaaa
    22021 ctttctttta taactaaata taattcttta tttctactaa ttctttcttc atatgattta
    22081 taaactaaag actcttttat tattactctt tcaatcatac atgaacaatg aaatataaac
    22141 tttactaaaa gttcatcttg aattactaaa gaatagtgtt cactaattct attaattact
    22201 tcttttaata taaaataagc cttttcccca tttaaaaaag tcaaagtttt actaatatta
    22261 tcaattagta atctatcaaa atatctacca ccattatctt tttcagcata tgtaacttcc
    22321 atatctccat aatgtttctt tatagattct ataatattaa aatataattt ctctaatgtc
    22381 atttctggag ttagagcctt tctagttgct tctattacaa taggtgtact taccatatct
    22441 attgctttta cttcttttcc tgtagcatca cgaataactt tagcaaatgt taaaatagac
    22501 cccatatcag ttaaaattaa aattcctttt cctttatcaa ttttattggc tatttctaca
    22561 gcttgtacta aaatatcctg aactctatct tctaatccca tattaattgc cactgcatgg
    22621 ttacaatcca gtaaagtatt ggctacattc accatgctag atgctgtaga ttcaccatgc
    22681 attatgacta atattgctat actctcctca tttaatccca catacgataa ataaagaaat
    22741 gttgctaaaa aaattgcttc atcttctgga aactctactc catacattaa tgacatgtta
    22801 gataatattt cttttgctat gttatattca tatgctcttt ccttcataat ttcatcttta
    22861 ttaggatgct ttactatatt tccaatcttt attctttcaa ttaaattctg aatatgtaaa
    22921 gctatagctt ttattaattt atctaatcct tctcttttgc tccatttatc atgcttttgt
    22981 aaaatatatt ttacagtagt tgcaattgaa tcattaacaa tgttgttata agctgtttga
    23041 ttactactat tatatataaa agtattcatt aaattatagg aatacttctg tatgtctcca
    23101 tctataacac ttctaatttg tgactcttta ataccttctt gttgaagttt attccaagtt
    23161 tcttttatca ttccataaaa atctaaatca tattttttat tttctattaa taaattttca
    23221 ctttcaaaat ccttttcttc tggtaaaaaa ataatatcat cttttccaat aaaactgaaa
    23281 ttttgtacaa actcctcttt tctattattt ccatataatg attctctaat attatttggt
    23341 agaagagaga gccttacata tacacattcc cttttatagg tcatataatc taaaaaagct
    23401 cttgcacaaa ttaattgtat atcgcttttt aattggccta tgtttccctt acacttatat
    23461 aaaataaatg ccttaataac ttcttttgaa acctttaact taacaccaat cctaacactc
    23521 tcttctttaa aaaaataaca tataagcttc attctctctt gaaagcctct ttcctctaaa
    23581 gacggaatct ttatagttac aggtattctt cttaaaaacg tttccaacat tgctgtttct
    23641 gggtcttctg tagtggctgc aattatcata actctacact cgtgtacctt attagcctct
    23701 cctaaccttc tatatatccc tttatccata agcagaaata gcatttcttg tccctctgca
    23761 gataatctat gaacttcatc taaaaacaat actccattat tagcttcatg tacaatacca
    23821 tacttatctt tatcagctcc agtaaatgct ccttttacat atccaaaaag ctgcgctaat
    23881 aataattgct gattatcaga ataatcagca cagttaaata tgataaatgg tgcattatca
    23941 ctatcttctt ttatagttaa actatactta tacatatatt ctgcaaatat agatttacca
    24001 actcctgtag gtcctattat taatgtactc aaccccttag gtggatatat tactgctgct
    24061 ttagcctgtt ctatttggga ccttaaactt ccatcacatc ctactaaatt aaaaatattg
    24121 ttctcttttt ttatactctc taaaatatac tcatctttga tagaatcatt ttcactatat
    24181 tcgtttatag agttaatatt atcaaatatc cctttattta gtttctttcc taaaatactc
    24241 tcaatacttt ctttatataa gtactgaaca ggcttaccct ttatctttat aataatactt
    24301 tcttttatta attgatttaa ttccttacta gcattacttc tatttatacc tacttcagta
    24361 gctatatatt ctgcatcaaa tccaaatcta atgccattat taaagttcaa gtgttcttct
    24421 gttaaccttt tcaagcaaat taaaactttt tctttccttg atagcttctt ttgtgtcata
    24481 gttaacctcc cctataagca gtaatactgt taaacattta ttattaagct taattctaca
    24541 atattttaat atttttgtct aattatgtaa ctattgattt tctttcctat tcgttttata
    24601 atataaatta cgttaccctc atcttttata gaggaggaat ttatatgaat tttttatcag
    24661 atttacacac ccactctata gtcagtggtc atggctatag cactttacta gaaaatatta
    24721 attattgtaa agaaaatgga attaagatac taggtacatc tgaacatggt cctaagatgc
    24781 ctggttctcc ccacaaatgg tactttcaca acattcaaaa tatcccaaga atcataaata
    24841 acattataat tttaagaggc tgtgaagcaa atatactaga tattaaagga aacatagacc
    24901 tagagccttt tgtaattcct cggttagatt atcttatctt gtcatttcac gaagctgtgt
    24961 tttctccaaa tacattagag aataatacta aagctttaat aaatgcaata aataagcatg
    25021 ataatattga aatattagga caccttggaa actcaaacta cccaatcgac tatgaattaa
    25081 tcattaaatt agctatagaa aagaatatct taatagaaat aaataattgt tctattaaag
    25141 gcgtgtcaag aaatggtagc tcggataact gtaagtatat tgctacactt tgcaaaaaat
    25201 atggtgctaa aatcatactg acttcagatg ctcacatttg ttttgacata ggtaactatg
    25261 agtattctga aaatatctta aaggaaatta attttccaga tgaattaata atgaattatc
    25321 ctaaaaaact tattaatcat tttcaccaaa aaggtaaatt actagatgtt gattatacaa
    25381 atatctaagt aataatacat gcttacttta actcccataa aaattttaat actatttata
    25441 ttatatgaaa taaagaaatt ataataaaga agctatttaa taaagcttaa ttacttttat
    25501 gaacttgatg gattgatatg gtctatgata gcaatagaaa ttataacatt tttaataaac
    25561 ttagttttat ataccttata gtataaatct aaaaaagcat catctaaaaa ggagctgtca
    25621 catgagcaaa ttaaatttgt tctatgatag ctcttttttt acttatttta attaggtat
//
LOCUS       DAELNY010000041        25420 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.041, whole genome shotgun sequence.
ACCESSION   DAELNY010000041 DAELNY010000000
VERSION     DAELNY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.041"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            224..1375
                     /locus_tag="KPE55_001443"
     CDS             224..1375
                     /locus_tag="KPE55_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492533.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1493..2131
                     /locus_tag="KPE55_001444"
     CDS             1493..2131
                     /locus_tag="KPE55_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0492534.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2132..3154
                     /locus_tag="KPE55_001445"
     CDS             2132..3154
                     /locus_tag="KPE55_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG0492535.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3144..3764
                     /locus_tag="KPE55_001446"
     CDS             3144..3764
                     /locus_tag="KPE55_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492536.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3888..4574)
                     /locus_tag="KPE55_001447"
     CDS             complement(3888..4574)
                     /locus_tag="KPE55_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG0492537.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4588..5133)
                     /locus_tag="KPE55_001448"
     CDS             complement(4588..5133)
                     /locus_tag="KPE55_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0492538.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5361..6608
                     /locus_tag="KPE55_001449"
     CDS             5361..6608
                     /locus_tag="KPE55_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0492539.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6695..8689
                     /locus_tag="KPE55_001450"
     CDS             6695..8689
                     /locus_tag="KPE55_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0492540.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8689..10011
                     /locus_tag="KPE55_001451"
     CDS             8689..10011
                     /locus_tag="KPE55_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0492541.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10290..11576
                     /locus_tag="KPE55_001452"
     CDS             10290..11576
                     /locus_tag="KPE55_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0492542.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11662..12969
                     /gene="grdB"
                     /locus_tag="KPE55_001453"
     CDS             11662..12969
                     /gene="grdB"
                     /locus_tag="KPE55_001453"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12709..12711,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0492543.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13247..13978
                     /locus_tag="KPE55_001454"
     CDS             13247..13978
                     /locus_tag="KPE55_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0492544.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            13980..15338
                     /locus_tag="KPE55_001455"
     CDS             13980..15338
                     /locus_tag="KPE55_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0492545.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            15377..15862
                     /locus_tag="KPE55_001456"
     CDS             15377..15862
                     /locus_tag="KPE55_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492546.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(16125..16361)
                     /locus_tag="KPE55_001457"
     CDS             complement(16125..16361)
                     /locus_tag="KPE55_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492547.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(16516..16740)
                     /locus_tag="KPE55_001458"
     CDS             complement(16516..16740)
                     /locus_tag="KPE55_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0492548.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(16837..18090)
                     /locus_tag="KPE55_001459"
     CDS             complement(16837..18090)
                     /locus_tag="KPE55_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG0492549.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            18350..19339
                     /locus_tag="KPE55_001460"
     CDS             18350..19339
                     /locus_tag="KPE55_001460"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG0492550.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            19411..20151
                     /locus_tag="KPE55_001461"
     CDS             19411..20151
                     /locus_tag="KPE55_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG0492551.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            20367..21518
                     /locus_tag="KPE55_001462"
     CDS             20367..21518
                     /locus_tag="KPE55_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0492552.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            21543..22298
                     /locus_tag="KPE55_001463"
     CDS             21543..22298
                     /locus_tag="KPE55_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG0492553.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            22490..24877
                     /locus_tag="KPE55_001464"
     CDS             22490..24877
                     /locus_tag="KPE55_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG0492554.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(25108..25341)
                     /locus_tag="KPE55_001465"
     CDS             complement(25108..25341)
                     /locus_tag="KPE55_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG0492555.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT        10175 a         2657 c         4280 g         8308 t
ORIGIN      
        1 agaacttagt catataaata tatagaaaat attttaatat attatacata acgggttatt
       61 ttataagttt aactataaat taggtttata ttttataaaa atttgctaaa aaaaacaatt
      121 aaaaattgta aagaaaaaag atataattag aactgtagta ataaatgttt tattatagat
      181 aatagagttt taagtatctt aaataatatt tggaggaata tgaatggaat taagaccaag
      241 aagtgcaaaa ttagaagaat ttgataaagt aatagaatta ataaactatg tatttagaat
      301 atcaagaaat cataaaccaa ctatgatgga agaatttcct cttttactaa gtaaaaataa
      361 tatagaaaat atgattataa tatcagaaga tgataaagtg gtttctgatg taaactatct
      421 aattcaggat gtatctattc aaggaaacag attgaaggtt gcagctatag gtggagtatg
      481 tactcatcca gattatgaaa agagaggcta ttcatctaag atattggata aagtagaaga
      541 aaaaatgttt tatgatggtg tagatattgt aattatatca ggaacaagaa gtctatacag
      601 tagaagaaat tgtagtttgg taaagagttt ttataaatat acaataaaac ctgaagatgt
      661 aaaaatagca tatgaaatag ttgaatttga tgaaactaat tttgaaaaag ataatgattt
      721 agataaaatg attgaactat ataatcaaaa tagtactaga tttataagaa cgagagatga
      781 gtttcaaaaa ctattgcatg cagcaactat tgcctgggga ccaattggtt acaagaaggt
      841 atttataaag gaaaataata atataatagg atatttgata ataagaacaa taaaaaaaga
      901 agattctact gtaggagaag ttgctgaaat agggttaaac tctgtaaatg ttgaaaatat
      961 attgaaatat gttgcaaata aatttggatt agaatcttta aattataaag tacatgtgaa
     1021 aaatcttaaa gaccagttga aatgtaatgg gacaaaatct ttggattatc aacaaggaac
     1081 tatgaaaata ataaatttta ctaagttgtg tgatagttta agaagttatt ttagtcaata
     1141 tgtagatttt gaattactaa aatacatgga atttaaacaa gttgaaaata aatatattat
     1201 taaatacaaa gaagaagaac ttgtaataga aaatttggat aagcttaata aactgttttt
     1261 tgaaaaaaat gaggaacagt acaatgaatt taaacatcta aaaaatatat atgaatttgc
     1321 tacaaaggca tttcctgtag attttccatg gactgcaaat ttaaattatc aatagtttta
     1381 gatgatatta aataaaattg attgatacct agagttagtc cttgttaaat aagagaaatt
     1441 attggatatt gaaaaagcaa ttttgtaagt aattagaaag gatgagtatt atatggacat
     1501 acttgatgat atagcagaat gtatattaaa tatgggtgta gataatatag agaatttagt
     1561 aaaaattgct atagataaaa atattgatgt ggaagatatt tatgaatatg gtttaaacaa
     1621 aggtatgata ggagctttag ataagtttga gaataaggaa tactatcttt cagaagttat
     1681 agtatgcaca gatgcattaa ataaaggtat taatcttttg aagggaactg gaaaagtaaa
     1741 gaaaaaatca aagggagtaa ttctgatgtc tgtagtagaa ggagatactc atgagatagg
     1801 aaaaaatatt gtaaaagtaa tggtagaagc cactggatat aaggttattg acttaggtgt
     1861 aaatagaaaa agtgaagata taatagaaga agctataaaa aacaatgtag atataatagg
     1921 cttatcttca atgatgacaa ctacaatgga aaatatgaaa tcggttatag acgaacttaa
     1981 tatgattgag atagataaaa gaccaaaagt aataattggt ggaggacctg taagtatgga
     2041 gtttgcagaa gaaataggag cagatggata cagttcaaat gctccaaaag cagttaaatt
     2101 aataaataaa cttataggag gtgaagttta aatgttttca gatatgactt tacttcagta
     2161 tataaatagt gaagatagag gtttttttct gcctgatatg ggtacaaatg gattgttctt
     2221 aataaataaa aaggcatatg aagtatatga tagtgtagat ttgcaattag aattagcaaa
     2281 gactatggac aagtattttg aatcagattt tatatactca ttctgtgatg ggattacatt
     2341 ttgtgaaact ctgggtttag atacattaag accagattat gattttccaa gtgtattgac
     2401 acatactata actgacagag ataaattaag aaaattagat gttccagacc cttatacgag
     2461 tggacgtatg ccactcaata tagagagctt atcagttata tcaaaaagta ttaaaaaacc
     2521 actatatgaa tcgatacaag gtccatttac acttgcaggt caattagctg gagctactca
     2581 acttttaaga tgtataataa ctgataaaaa atttgtagaa gaattattgg aatttacaac
     2641 tgaattggta agaagatatg ctgtagctgc aaataaagca ggagcaaaat atatttcaat
     2701 agcagagcca acatcagtaa ccctaagtaa agatagattt gatgaattta tagtaaaaaa
     2761 tttaaataaa atctacaatg aacttgattg ttggaaaggt atgcatatat gtggagacac
     2821 cagagaatta ttagataata tgctaagttg taatatagat gcagtaagtt tagaccaaat
     2881 tttagactat gaagaaattg ctccaatgat tcctagagat atagttttga ttggtaattt
     2941 agacccaata aaacttcttg gacgttctaa gccagataag attagaagag agacactaaa
     3001 attgttaaag aagatgagaa gatatgataa ctttttatgt gattttggct gtaactgctt
     3061 aaataccact ccagtagaaa atttacaagc agctataaag gcaggaagaa ttagttataa
     3121 agaattagat aggattgata ttgatgaatt ataatagtaa taaaaataaa tatcttttaa
     3181 aggatataaa tttagatgat atttttatag ataagaatat tgtgctaaag tttttggggt
     3241 atggaaatag aaaagctcca gatacaatta gtaaaatcgt tgaaagagaa ataagaaata
     3301 taaatgatat tttagatatc aagatatata taagtgaagt tgatattaat aaaacaccta
     3361 aagaatgtaa aaaagcattt gctgtactat atacaatagg ggataaaatt gattttaaga
     3421 tgaatgacta tatggaaaat tgcaatatga tggcagggct ggcattggat aaggtaggta
     3481 tagtatgttt agattgtatt aatgagaaaa taaaaatgta tttaaaagga aagtatgata
     3541 gtctcaaaat atcgcatgaa atatatccag gagataaaga ttttgaagta gagagacaaa
     3601 aggatattta taactatgta aaaaataaat ataatagtat agaaatagaa attaatgatt
     3661 atcatcaact atcccccata aaaagtgttg caatgcttat tttaatgggg gatgaagaaa
     3721 atttagaatc aagatgtagt aagtgtccta gaaaatgctt ttagttaatt ttcaagacta
     3781 agatttgttt tgtgtataaa ttgcaataat ttttttggtc attttatgta taaactaaaa
     3841 aaattattgc aaagtaatat tctatttaat ttgaacagac ttacaaacta tagtagtttc
     3901 tttaattcaa taagtgtatt tatttcataa gttggtatat gattagtata atttatactg
     3961 ttattggaat ttaaccaaca agtatctatt cctgaattta ttccacctaa gatatcagag
     4021 gttaagctat ccccaattat taaggcagaa gatttatcat gacaattaaa ctttttgaga
     4081 gcgtatttaa atatttcagg atttggtttt gatattttca actcttcaga tacaaccatt
     4141 ccatcaatat attcttttat ttttgaattt tcaagtcttt tcttttgaac tgcagaaatt
     4201 ccatttgtta ttataacaat agtatacttt tttttaagat attgtaatat atcgattgca
     4261 cctggaatta aaaatgtaca ttcacctaaa aaatttaagt acattttgct aagagtttca
     4321 gaatcaacat ccaaagatat tttatttgca aaaagttcaa atctaagtaa tttaagttca
     4381 ttcaaagtta taatattttt ttcaaggtcg agccaaagct tatcacttat atttctatat
     4441 gactctatat aattctcaaa attaaattct aaatcaaact cagatagaag ttttttaaaa
     4501 gcatgtgatt cacttttctt aaaatcaaat aaagtatcat ctgcatcaaa aaatataaat
     4561 ttatatgtct tcatggtttt tactccttta caagatgact gcaaaaatta ctttactttc
     4621 actatttgag tcattttgcc atttatgttt tgttcctaaa ggaactttta cactatctcc
     4681 tttggacaaa gtaaattcat catcatccat aaaaagttta acttctccct ccaaaacata
     4741 tgcaatttca tctccattat gagtaatcct atcaacacaa gaagaagtgt ttggagctaa
     4801 atccataatt gcaaattcta aattaccact agagtctgga attaaaatct catatataac
     4861 ttctttgctt ccaggtaaaa taactttttt tctattatca tgtcttacta tcaagttttt
     4921 tttatctacc tcagatacaa aaaatgtaaa taaaggaacg tttagagaag aggctataga
     4981 ttttaatgaa tttacagatg gattagcaag acctcgttct atttggctca ataatgatgg
     5041 tgtaacatca gctagttttg ctaaatctcg aatacttaag ttttgttttt ttcgaacttc
     5101 agctattttt tcccctaaat ttaaatcatt catataaata tcctctcttt ttaatttaaa
     5161 aaatattaac ttaaaataat caagtaaaat gattatgtag tatgaaatta ttataacata
     5221 tagaattagg ttttgaaaaa actatgttca atataattaa ataaattaaa atataattga
     5281 caaagtggat acagaatact taaaatatat ttaaataaaa ttaagcaaaa gatagttaat
     5341 ttatgatagg aggggatatt atgcaaataa cgacttttgg agctatattt ggtttattaa
     5401 tagcaattat tttaattata aaaaaatttc aagctgtata tagtttaatg ctaggagcat
     5461 ttattggagg attagttgga ggagcaaata taacacaaac tgtagatttt atggcaaatg
     5521 gagctatgaa tatatcacca tcaattctta gagctttggc atcaggtgta ttagctggaa
     5581 gtttaataaa aacaggggca gtagataaaa tatcagaaca gattgtaaaa atatttggtg
     5641 aaaagagggc tttattttcg attgccattt caactatggt acttgctgga gtaggcgtaa
     5701 atttggatgt atctataata actgtagcac ctataggatt atatataggt agaaaattaa
     5761 attattctaa attgtcaata ctattagcta tgttgggagg gggtaaagca ggtaatataa
     5821 tatcaccaaa tccaaatact atagcagtag cagataattt ttctgtcaat ctctctagtg
     5881 ttatgatggc aaatataatt ccagcaatta ttggagttgt tattacagtt attttagcaa
     5941 gtatattaat aaataaagga aataaagtac aatcatatga aattttagag caaagagaag
     6001 atttaccaag tttatttaaa tcattatgtg gtccaattat agctatattt ctattgtttt
     6061 taggaaatgt atctcctata gttatagacc caatgatagc ccttccaata ggaggaatcg
     6121 taactctaat tgtcacagga aacttgaaca attcaagaga atatttagct tttggattat
     6181 ctaagatgca aggggtatgc atattattac ttggaacagg gactatcgca ggaattatac
     6241 agatgtctga attacaacag agtacaatag gtgctttaca atttttaaat atgccacaat
     6301 ttttattggc accagtttcg ggtatactta tgtcattagc aacagcatca tcaacagctg
     6361 gtgctactat agcatcatct acttttcatg atgctataat aaatggtggg ctatcaccga
     6421 tttcaggtgc ctcaatagta aatgctgggt ctagtgtatt cgagcaatta ccacatggct
     6481 cactatttca tactagtgca ggtagtataa acatggatat aggtgaaaga tttaagttaa
     6541 ttccatatga agcattgata ggaatagtaa tgactataat ttcaacatct attcaattag
     6601 ttttataata aatataaata tttaatgaaa aaagtaggat tttgttatta aatattgaat
     6661 ttattatagt aaaaaagaag attggagttt taaagtgaat aaaaaaaaga ttgttattat
     6721 agggattatt tattcatttt tagtagtatt ttcacttaca aatatgtatg taaatatgga
     6781 gtataatcta aatgtatttg aatatattaa gaagtcactt ccatttacag aagaagaaaa
     6841 aaagtggtta gaaaaacaca agaatttgat ttacagctct gaccaaagtt cacctccttt
     6901 gagatataaa ggtaaggaag atggacagta taagggaatt attgttgatt taataaattc
     6961 tctgtcaatt caaattggta gagattttta ttttaaacca aataattggt ggaaagaatc
     7021 tttcgtaaat tcaatagatg atagtataaa gttttttgac ttgattccat caaaagaaag
     7081 ggcaaataaa tttatattta ctgacccaat atacactttg agtgcaaata tattgaaaga
     7141 taaaaagtcc caagatataa atagttacat ggatttaaag gggaaaacag tagcaattcc
     7201 agaaggtgat tattctataa attttttaaa gcaaaaaatt caagatataa acattttgtt
     7261 gactccagat ataaagactg gagtcaacca tttaatgtct ggaaaagtag acgcagttgt
     7321 tggtgatgaa ccagtattgc gatactatat aaataattat ggactttcaa ataaatacag
     7381 tgttttaagt aatcctatat atactaaaaa agcagttctt gctgtaccta aacaatatga
     7441 agagctggtt agtattttaa ataaaggcat ttttaaactt cagaaaaatg gagtgtataa
     7501 agatttaaag aaaaaatggt attctacgta taatgaagtt gatgatatat tgtatgagcg
     7561 aggaattgtg cctagtattt atctttttat aggtataatt ttaatatcaa tttatgtttt
     7621 ttatagctat acatatttgt taaagataga aatcaaaagg aaaacagaac aggtaataga
     7681 aaataaaaaa actttagagg caacttttaa tagtattaca gatataataa tgcttgtaga
     7741 tgaaaataac aatatagtag aatcgaacaa agtactatat gactttatgg gtgaaatgag
     7801 ttataagata gcagatttga ttagcatgat aaaaggtgtt attgaaaata ccttctctga
     7861 aaatactaat aaaactagtg aaatagaaat acacaataaa attttaaaga taaacacttt
     7921 tcctgtagag tataaaaaaa ataatactga gtatatagta gttcttatta aagatataac
     7981 taatgataaa atagtagagg cgaaattact tagagaaaac aaaatgatat ctataggaca
     8041 attagcttct ggggtagcac atgagataag aaatcctttg ggtataataa gaaataattg
     8101 ctatctctta aaagataacg ttacaatgga agaagtaaat gattgtgtta aatcaataga
     8161 aagtaatgta gatagggcaa gtaatataat aaccaacttg ttaaatttcg caagaatatc
     8221 agatgataat ttagaacata taaatataag aaattttatt gaaaatatag tgaaacttca
     8281 atataaaatg ttacagttaa agaatgttga aataaaaatt gactgtgaac ataatttagt
     8341 atgctatata aatggagaat cgttaaaaca tgtatttata aatttaattt ctaattcaat
     8401 agatgcaata catcaagatg gaaaaattat tatatactgt tatgaaaaaa attattgttt
     8461 atttatagat tttaaagata atggtgaagg aataaaagaa gatgccctaa aggacatatt
     8521 taatcccttc tatacaacaa aaccaattgg tgagggaact ggtttaggtt tatacattac
     8581 ttataatgag attaaaaaga ataatggaga tataagtgtt gaaagtaaat tgggggttgg
     8641 aacttgtttt catataaaaa ttccattaaa taaagaggtg actatataat gaaaatacta
     8701 ttagttgatg atgagctaga atatggagtg gtaatgaaaa aaattctaca aaagaagggc
     8761 tatcttgtag atatagcact aagtggtgaa gaagctataa atataattaa aaaagataaa
     8821 aattatgatt tggttttgtc tgatgttatg atgaaaaata tggatggagt acagctatta
     8881 gatagaatta aatctattaa tagagatata gaggttatat tagttacagg atatggaagt
     8941 attgaaaatg cagttgatgc tatgaaaaga ggagctttat cttattttat aaaaagtaat
     9001 cccattgaaa atttattaga agaagttgaa aaggtaaagg caagtaaaat atctgtttct
     9061 gtacaaaaaa ataatctgga atttactttg gaatctaaaa atagagattt taataatatc
     9121 ataaaaatag caaaaaaagc tgcttgtaaa gatgttaata tattaatctt gggggaatct
     9181 ggtgtaggaa aagatatatt agctagatat atacatagca taagtccaag aaagaatgag
     9241 atatttgtac ctgtaaattg ttgttctttt tcagaaaatc tattggagtc tgagttgttt
     9301 ggtcatgaaa aaggttcttt tacaggagca gtagatagta gaaaaggtag atttgaactt
     9361 tcgaataaag gaactctatt tttggatgaa ataggagata tacctctaaa tgttcaggta
     9421 aaacttttga ggacacttga ggacaaatct atagagagga taggaagtaa taaatctatt
     9481 aaagttgact ttaggttaat ttgtgctatg aataaagaac caaaagtgga aatatcaaat
     9541 ggaaatataa gagaagattt tttttataga ataagtacaa taacaataac tataccaccg
     9601 ctaagaaaga gaagagaaga cttaactact cttatagaat ttttcttaaa taagtaccaa
     9661 attgaacatg ataaaaagat acactcgatt gataaggaag taaaggattt tttattaaat
     9721 tacaattatc caggaaatat aagagaatta aaaaatataa taaatagact tgtagtttta
     9781 agtgaagaag gcaatttatc aaaagacaat ttaaatttaa tatcaaataa tgtatacata
     9841 gatgataaaa taagtattag acctttaaga gaaataagaa aagagtttga atgtgagtat
     9901 attgaaaaag ttttatcttt gtgtggaaat aatatttcaa atacagctaa aaaattggag
     9961 attagtagaa gacagttaac caataaaata tcagagtata atattaagtg agaaaaaaac
    10021 ttctcattaa gtgagaaaaa aatttctcaa actgtattaa aagataaaca gaaaaaatct
    10081 gtttatcttt ttttattata attttaaaaa ttaatattta caataattat tgctaaattc
    10141 aatattggtg taaattaaaa atgaaaattt ctatgacatt aatacataga atatatattt
    10201 taaataattg gcatagaaaa tgctttatat attaataaac gtaataaata cttttaattg
    10261 cgattaaata tattaagggg gattttcata tgaagctaga gctagggaat atatttataa
    10321 aaaatgttga atttggagaa aagacagaag taaaagatgg agtactatat attagtagcg
    10381 aagaaataga aaagatagta ttagaagatg aaaggattgt aagtgtaaat gttgaacttg
    10441 caagacctgg cgaatcaata agaatagctc cagttaaaga tgtaatagag cctagagtta
    10501 aaattggaga tgaaagcaaa atatttcctg gaattataaa taaagtgaag actgtaggaa
    10561 gtggaagaac ccatgtactt ttaggagctt gtgttgtaac ttgtggaaat atagttggat
    10621 ttcaagaagg cgtaattgat atgagtggtc ctactgctaa atatacacct ttttcaaaaa
    10681 caaataatat atgtattgtt ataaaagcaa aggatggtat agatactcat gattatgaag
    10741 aagcagctag aattgcagga ttaaaaatag gtgcatatgt aggtgaggca ggaagagaag
    10801 ttgagccaga tgaagtcatt gtatatgaga caaagccatt attaaaacaa gtaaaagaat
    10861 atccagacct gccaacagta gcatatgttc acatgttaca atcacaagga cttttacatg
    10921 acacttatta ctatggggtg gatgctaaac agatagtgcc tacatttatg tatccaactg
    10981 aaataatgga tggagctata atatctggaa actgtgtagc accatgtgat aaggttacaa
    11041 cattccatca tttaaataat ccagttatac atgacttata taaaagacat ggaaaagatt
    11101 taaactttat aggagttatt ttgactaatg agaacgtatt cttagtcgat aaagagagaa
    11161 gctctgatat ggtagctaaa ttaatagaat ttttaggagt tgatggagtt ctagtgacag
    11221 aagaaggtta tgggaatcca gatactgacc taatgatgaa ttgtagaaaa tgtagtgaag
    11281 ttggagcaaa tgtagttttg ataacggatg aatttcctgg aaaagatggc aagagtcaat
    11341 ctatagcaga cgctactaag gaagcagatg cagttgtttc ttgtggtcaa ggaaatctag
    11401 tggttcattt cccagcaatg gaaaaaatta taggaacttt agattatgtt gaaatgatga
    11461 taggtgggta taaaggatgc ttaaatgaag acggaagcat ggatgcagag ctacaaatta
    11521 taatagcatc aactatagca aatggctata atcatttgac agcaagatat tattaaataa
    11581 gtatttgaat ataaagttct gttgatataa tcattggtct aattgatgta acaactataa
    11641 agtgatttgg aggattaaga gatgagtaaa atcagagtgg ttcattatat aaacaatttc
    11701 tttgctggta taggtggaga ggaaaaagca gatattccgc cagaaaaaag ggctggtgta
    11761 gtaggacctg gaatagcatt tcagagtcaa tttaatgatg atgcagagat aatctcaact
    11821 gtaatttgcg gagatacgta ttttggtgaa aatattgaga gtgcaactca agaactttta
    11881 aaaatgataa aagaagaaag ccctgattta tttattgcag gcccagcatt taatgcaggt
    11941 agatatggag tagcttgtgg gactatttgt aaggcagttg aagaagagtt aaatattcct
    12001 gtaattacag gcatgtataa agaaaatcca ggtgtagata tgtttaaatt agatttgcat
    12061 atagtgtcta caggaaattc agcagcaagt cttagaaagg tagttccaat aatgacaaaa
    12121 ctaggtctta aattggttag aggtgaggaa ataggacctc ctgaagagga aggatatata
    12181 atgagaggaa taagaaaaaa tttcttccat gaacttagag ggtctgaaag agcaattgat
    12241 atgttggtta aaaagatgaa aggggaaagc tttgaaactg agtatccaat gcctgaattt
    12301 gatagagtta cacctatgaa acctgttaaa gatttatcta agataaaact tgccctagta
    12361 acttctggag ggatagttcc aattgataat ccagacaaaa ttgaatcttc aagtgctact
    12421 aaatatggaa tttacgattt aacagatatg gattctatgt caaataaaga atttacaact
    12481 atacatgggg ggtatgacag agcatatgtc cttgaaaatc caaatttagt tgtaccacta
    12541 gatgtagtta gagagcttga aaaagaaggt gtaattggag aattagtaga ttattttata
    12601 actactacag gaacaggaac gagtgttggc aattctaaga gatttggaga agagttttct
    12661 aaaaaattgt tagaagatga tgtagatgct gtaattttaa cttctacatg aggtacttgt
    12721 actcgttgtg gagcaacgat ggtaaaagag attgagagag ctggtatagc agttgttcat
    12781 atatgtacag tagtaccaat atcacttaca ataggagcta atagaattgt tccagcagta
    12841 gggataccat atccacttgg aaatcctatg ttaggagaag aggaaagtaa aaaaattaga
    12901 aagaatatag ttttaaaagc tttaaatgca ctaacagaag atatagaaga acaaacagta
    12961 tttgaataat aaaaatacaa cacaacactg aaataaaaaa agatatcaaa aaataatttt
    13021 ttaaaattat tttacgatat ctttttttta ttaggtaaat tcgaaatttg cattttttaa
    13081 aattaaattt ataaaagtga taaattagtt tattataata ttgatacaca aattcaaatt
    13141 agatatacta tgaaaagcga gattattaca ttttggaaca aatattcaac atattagatc
    13201 atattaattg aacagaaaaa attaacttta tataataatt agaataatga taagaaagaa
    13261 ggtgtatata ttgatttcaa ttgccatttg tgatgatgaa tcctatatgc taaatcattt
    13321 aaaggaaaat atagaattat acatgaagca gaagaattta ttatataata tagagctttt
    13381 taataaagta gaagatttac tatgcaatca tgaaaagttt aatattgtat ttttagatat
    13441 acagatggaa gagattaacg gaatgcaggc agctagaaaa ttaaggtcat atggtaatga
    13501 gtgctttata attttcgtta cagttttaaa tgaatttgta tttgatgctt ttgaagtaga
    13561 tgctataaat tatctgataa aacctctatc taatgagaaa ctatataaaa ctttggataa
    13621 aatagttaca aaacttgatg ataatgaaag taattatatc acaatacaaa aaggttattc
    13681 atattggaaa ttaaaacttg ctgatatttt atattgtgaa gttataaaaa gaaagattta
    13741 tgttcattta aagaaagaga ctgtagatta ttatgagact attgaaaatt tagagagaag
    13801 tttaccaaca aattttttta ggtgtcatcg tagttatata atcaatttac aatatgtata
    13861 tggatatgaa aatggtaatg cagttatgga aagtggagat agaatacctg tttctagact
    13921 acgtcaacaa atattttcaa aagttatgct tgagttcatg aaaaatagga ggatataaga
    13981 tgattaattt attttcttcc gatttatttt taaagtacag ttttttattt tcttttattg
    14041 tatatagaat tttaatggta atagtgcaat actattttct aatcagtttt acaaatgtta
    14101 attcaaaatt ttggtgtttt attttatatt cgataatttc aattttttta atcattataa
    14161 atacatattt taatttatat aatttatttt cttcacagat tctatttttt cttatgactt
    14221 ttttatttgt aaagtacata ataaaacaat ctactgtttt atctatagta tcaactttgt
    14281 tagtatattt aattgaacaa ttagtatatg gatttacagc tccattaaat tacttgatat
    14341 ttactagtga tataaataca gatatttata aatttgatat aaatatggga ctatcttctt
    14401 taattactat tataatagct ggatttatat attggtatag ttctaaaaag tttaatatta
    14461 aggtcatgaa ctttgataaa tatatagcaa ttttgatgat tccattgttg cttataatat
    14521 tatttatgca atcatttgaa tatagttcaa atataaatat agatacaagt cttgggattg
    14581 taaagctgct attaaatact tctataactg aagaaattca agcttttctt ttttctataa
    14641 ttggaactat atgtgtattt ttttctctat ttacctttaa gaaactaatt caagctctcg
    14701 aagatgacaa ggagagggca attatgaatc aacagattca tgctcaaaaa aactatattg
    14761 aagaagcgaa atcacgatta tcacaaacta tatcctttag acatgatttt aataatcatt
    14821 tagctattgt caatggattg ttgaaaaaag accaaatttt aaaagcacaa gattatttaa
    14881 ataagcttga aaaagtaaca agtgacctat cattttcttg taatacacag aatattgtta
    14941 ttgatgctct tttcaacaat aaattaagta tagcaaaaca attaggcata gaggtagatt
    15001 gtgaagtaat tataccccaa agcactaaca tgaacgattt tgacctatgt atagtgtttg
    15061 ctaatgctat tgacaatgca attaaagctt gtagagttgt agataaacaa aataggtatt
    15121 taaagctttc cactaagtta gagggtggat tttttatgat agaaataaaa aatagctata
    15181 atccaataaa taattataca agtggctcag gtataggtct tttaaatata aaagaaattg
    15241 ctaaaaaata tcaaggggct ataagtatag ataagacatc agattatttt caaataaatg
    15301 tattgctggt tatttcatta cattgcgcta gtatttgata acaatttcat tgaaaatagt
    15361 atttgtaagc tatattatgt acttgaatag agaaagtttg atatatagaa atgaggaggt
    15421 tactaaaatg aaagttggaa atataaatgg tcaagcacca attacaaatg gtttagatag
    15481 ttgtcaaagt gaggtacaag gcttagaaca aaagaaaaaa tcattacagg atgaaataga
    15541 gaaagtaaga agtgataaaa gtattgatgg tgaaacagcc aaattaaaaa tagaagcttt
    15601 aaaaaaagaa atggaagaga ttgaaaataa aattcaagag ttaaaaggaa ataaagcaaa
    15661 aaaaacaaat aaagaagaaa ataatgtttc agaagtaaat aataatctat tagagagaaa
    15721 aaaagaacct tctaataatg aaaatgtaaa acaattatat gatgtttttg aaagaagcaa
    15781 tgtagacgaa caatgggata atacttatcg tgttctccaa gatgataaaa acttaaaagt
    15841 aaaatttaat agacctatat agtttattta taaaaatagt taatatttta attatcacca
    15901 ctttttagaa gaatacacac tttattttaa agacccacat atttatttac agttagcaca
    15961 tttatatgtg gttttataac tattatttac aagtctatat tgataacttt tacatagtat
    16021 ataatgttta aatacttatt caaatttatc tatacataaa caaaaaaaga gattttaaaa
    16081 tctcttttta gattgttgac ttatggcttt ttgatttaat aaaatcaaga agccgtttga
    16141 attttatctg ttggctgata cccttctgat attgaaacac catttttgtt tccaaaatcg
    16201 tttaaaatta agaacttatt atgaatgact atttcattat gaacaataag cagataacat
    16261 gaatcttcaa tcaatgtttt aacagtagca ttaaatgctt gcttatacag ttctatcgtt
    16321 tgtttgaact gtccattaaa gtaaatatga gtcaataaca taactatatc tacacaaatt
    16381 aatataaatt ttttatactg tacattatct aactgttgat tatctgtatt acaacatgat
    16441 tgaatttaaa aagccatcaa cacatagaat gttgatggcc tgaaaataga tttaaaatct
    16501 cttttttatt atatattaat tattttttaa atttatattt tttgcatcag attttctaat
    16561 agctaagtca aaccctctaa atctaatcac tatagggtca ttaagaggag ctatcttttt
    16621 cacttcaact tcagtgccat ctattagtcc taaggctaga agtctcttca taagtttttc
    16681 atttccagct atattgtcta taattccctt ttgtccaagt tttaagttgt aaacagtcat
    16741 tattaacacc tcctaatgaa aatcattatc aaactttata tttataatat ataacaaaaa
    16801 gagcaaattg tcaatatata acaaaaaata aacatactaa gttagtatgt ttataaaaaa
    16861 tagtatacat gggatactaa tagcatctag caaaaaacca cttactaatg gtacaactaa
    16921 aaaggctttt ggagaatcac catatttttg tgtcaaagaa ctcatatttg ccagagcgtt
    16981 tggagtagct cctattccat gtccaatcaa accacttatt atgatggcag aatcaaaatc
    17041 ttttccaagt actctaaagc aaataaatat tgaaaatagg attataaaga ttacttgaaa
    17101 agttactatt aagagaatag gaggtaataa tgtagataat tcaaataaat ctatattcat
    17161 aagtgataaa gttaaaaaaa ttcccaatga aagttctttt aaaaaatcta taagtttaaa
    17221 atttaattct agtatattta tcttatcatt taaatttcta aaaataacag ccgaaaacat
    17281 acaagttaca acagagggta atagtatatt aaacgttata tagaagcatc tacttataat
    17341 ttctcctgta tttatacaaa taaatatgag aagaacctgt tccaaaaata cagatggtgt
    17401 tattgtttcg gagaagttat tattttttat cctattctta tttctaaaaa atttacttcc
    17461 aaatctcttt agatttctat tatatctagg aatcgacaag tctatatttg atgatggttt
    17521 taatttatat ttatttatta gaaatttagc tacaggacat cccaatatac cacccataat
    17581 aagtcctatt gttgcagcag ataatccaac cccaactgct ccttctatac cgagattttc
    17641 tattgtaagt ccaaatgcag cagaataacc atgaccacct tccatagata catttccaca
    17701 cataagccct aaaagaggtt caagttttat aatctttgat attgtaatag ctaatatatt
    17761 ttgacaaaat gccaatactc cacacaaaat ccaatatcta aataatacac taccaccttt
    17821 ctttataaga tttatgctta tttcaagtcc tatagttgta aaaaagaagg ttatgaaatt
    17881 tggcattaaa tttccactta tagatatatc tgctatatta aaatatttaa aaaataaatt
    17941 tagaaaacaa aataatagac ctcctattac tggtgcaggt atacagaaat tatttaaaaa
    18001 gttaacttta ttcttcaata aattaccaat taaaaagaag aatatagata gtgtcaaggt
    18061 ttgcactata tcaaaactta aggtaatcaa aataagtcct cctttgtaat tcaaagtttt
    18121 aattatataa cttaaattct tatgtattga aattttatta tcaaatgtat attaataaat
    18181 gtatattaaa aagaatcagt agatataatt agatagtcat aaattaaatt aaaatatttt
    18241 aatgagatat gactttttta gaattaaaaa ccgtagtata ttgatataat agaaataaga
    18301 cttaaaaatc tgttgatagt attatttata agtacagtag gaggataaaa tgtttgaaaa
    18361 tttacctaag atagatttac attgccactt agatggaagt gttagagtag aaaccatgct
    18421 tgatatagct attaaagaga aaatagattt accttcaaat aatatggatg aaataaaaaa
    18481 attagcaaag gtttctttta attgtacttc actagatgaa tatttagaaa aatttgattt
    18541 acccttaaaa gtaatgcaat ctaaggaaaa tttaaaaaga attacttttg aacttttgga
    18601 agatgcaagt agagaaaatg taaaatatat tgaaattaga tttgcaccat tacttcacac
    18661 ccaaaaaggt atgagtgtta aaaatataat tgagggtata attgaaggaa ttagagaagc
    18721 tgaaagtatt tatgatataa aaggcaattt aatattaggt tgtatgagaa caatgacctc
    18781 aaaagaggca ttattagtaa ttgaagaagg gaaatcattt gtaaataaag gtgttgtagc
    18841 agtagacttg tgtggtccag agaaagaggg attttgtaag gaatataaag atgtttttaa
    18901 attagcaaga gaatatggat ataaagttac aatacatgca ggagaagctg caagtggaga
    18961 aaatgtattg gatgctataa atattttaaa ggcggatagg attggtcatg gggtaaaaat
    19021 taaagaccat aaaaaggcgt ataatttagt taaagataaa aaaatattgc ttgaattatg
    19081 tccaactagt aatgtacaaa ctaagacagt agattcttat gaagcacatc cattttatac
    19141 tttttataaa gataatttac atgtaagtat aaatactgat aatagaactg tatcagatat
    19201 aaatttaagt tcagaactaa atgttatatt tgatactttt aaactaggat tagaagacta
    19261 taaaataatt tatagaaatg cagtagaagc tagttttgca gataaagaaa ctaaagagta
    19321 tttgaatagt ttaatttaat agataaaaat atttaaactt taaattaagc tattaaattg
    19381 ataaaaatac attgtattag gagaaaaaat gtgaaagatt atgaagatgt tgtttatatt
    19441 aaactagtca aaaaagaact tgaagaatgg aagaaaaata tattaaagaa accttctata
    19501 tttgacaaag catcaaaggg tatacagaat aaatttaatg gagtattgcc agataaatat
    19561 catgaaataa ttacttcttc tataaaagta atgacaaaaa ccgtactgtt tggatataaa
    19621 tatgctacaa agccaccact tagaaatatg tcattagaag aaagagaaaa attagtttta
    19681 gaaaaagcta aaacttacaa gactacagca atgattgaag gggcaggaac aggggcagga
    19741 ggaatcctct taggtttatc tgattttcct ttgcttttag gtattaaagt gaaattttta
    19801 tatgatgtag cttcaatata tggatatgat gttaaagatt atagagaaag gatttatata
    19861 ttaaatatat tacagttggc tttttctagt caacagcata taaacaaaac ttttaaattg
    19921 atgaataact gggatgaata tgtaaatagt ctaccagaag acatcaatga ttttgattgg
    19981 agaacatttc aacaagagta tagggattat ttagatttag ctaagttttt acaactaatg
    20041 cctggaatag gagcatttgt aggattttat gtgaatggaa aattaattga taaattatgt
    20101 gaaactgctg tgtctgctta tagattgagg attcgagagc ttaagttata agaaaaatta
    20161 taacataaaa aattagttat acaatatatc tatatgtatc taattttgca ttgataaaaa
    20221 caattatgga taaaaggtaa agcatgatag aatctaatgt agaggtttgt attttaatgg
    20281 aaaaatttag tttttattgc ttaagataca aattgaatta cgaagagatt gaattatgaa
    20341 gagaagtaat tgggaggaat attttcatga caaatttgcc aaaacaattt gatagcttca
    20401 atgaagctag acaaaaagga tttataaatg caaaggaact taaggaaagt gggaaaaaga
    20461 tggttggagt tttctgtaca tttactccag ttgaaattcc aatggcagct ggagcaactg
    20521 tagtaggtgt atgcggcgtt agtgaagagc caatacctga tgcagaaagg gtgcttccaa
    20581 gaaatctttg tcctttaatt aaatcaagct atggacatgc aataacagat acctgtcctt
    20641 atttctattt ttctgatttg ttgataggag aaaccacttg tgatggtaag aaaaaaatgt
    20701 atgaagaact tgcaaaagtt aagccaacat atgtaatgca tttgccaaat acttcaaaag
    20761 gagaatttgc ctataaactt tggaaagatg aaatgataag attaaaagaa gaggttgaga
    20821 aatctttagg tgtaactata actgaagaag atataagaac tgctataaaa gataaaaatg
    20881 aagaaagaga acttttaaaa gagttttatg cacttggtaa attacaacca tctgcactta
    20941 caggccttga gttacataat gttttatatc aagcaggttt taaatttgac cgtgcagaac
    21001 ttaaacattc tttaagaaaa gttatagatg atatgaaaga aagatatgaa aaaggtgaat
    21061 gtcctgttca aaaagataag ccaagaatat tgataacagg ttctcctatt ggtggaataa
    21121 gcgaaaaaat agttaaaact ttagaagatg caggggcatc agtcgttgca tatgaacttt
    21181 gtggagctat tagatcaaac gatttattgg ttgatgaaga aatagaagat gtatatgatg
    21241 ctttaacaca aaaatatata aatataggtt gttcttgtat gatgaataat gacaatcgta
    21301 ttgaattatt agatagaata atagatgaat ataatgtaga tgctgtaata gatgtagttt
    21361 tacaagcatg tcatacattt aatatagaaa gttatagaat aagagaattt gttacaaaag
    21421 aaaaaaataa gccatttatg tctcttgaaa ctgattattc aaaatcagat actgaacaat
    21481 taagaactag atttgaagcg tttgtagaga tgttgtagga ataaataaaa gtaggaggaa
    21541 ctatgtttag tataggagtt gactcagggt cagtggcaac aaagggtgtg ctatttgatg
    21601 gtgaaaaaat tattaagaaa ataattatac caactggatg gagccctaaa agtacatcta
    21661 aacaggtcta tgagttgcta agtagtgaaa ttgacaaaaa agatataaaa aaggtggtag
    21721 gtacaggtta tggaagggga gtaatggatt ttgctgataa gaaagtaaca gaaataactt
    21781 gtcatactag aggtatttac tttttaaaca agaacataag gactatttta gatgtaggtg
    21841 gtcaagatag taaagttata aatttagata gagatggaaa tgtatttaac tttataatga
    21901 atgataagtg tgctgcagga actggtcgat ttttagagat tacttctaac cttttaggaa
    21961 gcgatattga aagtatagat acattagcta aaggatatga accagttaat atatctagta
    22021 tgtgtacagt ttttgctgag tctgaaattg taagtcttct agctcaaaac atttctactg
    22081 gagaggtagc agcagggata cttaaatcta ttgcaaataa atccacctca atgttagcaa
    22141 gaggtgaggt aatagatgaa gttgcattta ctggagggct tgcaaaaagt aaagaactag
    22201 taaaaatgat agaagaaata ctgggtaaaa aaatttttat tgcagaagat actcaaataa
    22261 taggagcttt aggagctgct gtaataggat ttagataaaa acatataaaa agaaaatgag
    22321 aagctgaatt tattataaat aaattcagtt ttttatttta ttttatttat ttataaagga
    22381 aaactttggt ataattgatt taaatgattg tatatttgta aatacttgat atttagtcat
    22441 atataacatt ataattttaa aaattagctc aaaataaggg ggagaaagcg tgaaaaaatt
    22501 tacttcaaaa aaagtaacaa ggtttgttgc gctgttttta atatctattt tagtaatgtc
    22561 aacaattcct gtatcagctg acaatagcaa tacattgaat cagataaaaa aagcagaata
    22621 cagtgagacg tataaacaat atctaaatga tgctaaaaat ggatgtacag aaaaatataa
    22681 tgggattata ccaaatccat atcagttgga aggtactcaa atccaaagta aaggaagaac
    22741 agctccaaca tcttatgacc ctagaaaatt aggtcttatg acatcaataa aaaatcaaga
    22801 ggatttagga atctgttggg attttgctgc aatggcaacc ttagaaagtt tcttaaagtt
    22861 aaataattat ggagactatg atttatcaga agaacactta agatggtggg cttcagatgg
    22921 tgaatatgga tggtctgtaa atgatatgaa tggtgcttta aattatgaag ctatgggata
    22981 tttaacatct tggtcaggtc ctaaattaga aaaagatatc ccatacaatg gtagagtatc
    23041 taaagcacaa ggagcaaaaa aaccaactaa tatgaataca gcacctactg tttttaatgt
    23101 gacagatgct gtgtgtgtaa gcaatgacat aaattctaca aaaaatgcta tattacagta
    23161 tggagctgta acatctggtt attatgaaga tattaaatat caaagtgatg accaaaattc
    23221 atattattgt ccaacaaaaa gttttaatac aaatcatgct atttcaattg taggttggga
    23281 tgataactat tctaaagata attttaatgc aaatattaga ccatcaaaaa atggtgcttg
    23341 gttaataaaa aatagctggg gagattataa ttctgagggt ggatatttct ggatatctta
    23401 tgaagacaag acacttatgt ctgatataga taatttttca atcaaaggtg gtaaaaaacc
    23461 aaatgatgat gagaagatgt atcaacatga ctatgcaagt attgtacctt taatttcaaa
    23521 aaagataaca gctgcaaatg tatttgattt caatagaggc gatgaaactc taaaatctgt
    23581 aatgttctta actgaaagta taggagctaa gtatgaagta tattatgctc cagtagtaaa
    23641 tggtatacct caagaaaaca atatgaaaaa attaaaagaa ggtactgccc aatattcagg
    23701 atacataaca gttccaattg attcttttga tataccagaa ggaaagggtg caattgtagt
    23761 cagtatagag gggaaaaatg gagagtcaac tataggttct gagtcaaatg taccaggata
    23821 tgatacattt aaagcaaaag ctaatttagg tgaaagttat atcatagata atactggaaa
    23881 attctttgat ataaatagag atagtaactt ctatccgtgt aattttacta taaaagctgt
    23941 tacagaaaag tcttctggtg agagtatccc aaatgagtct ttaattggta gtgatagata
    24001 tgaaactgct atcaaagtta gtcaaaatgg tttcaattct tcagaaaatg tagttttagt
    24061 aaatggcagt tcaatagtag atgcattggc agcaactcct tttacatctg ctataaattc
    24121 accaatatta ttgacacaaa aagaagcttt aaattcaaaa actaaagctg agatacagag
    24181 attaggtgct aagaaagtat acctaatagg tggagaaaat tctataagta aagaaattga
    24241 acaacaatta aaaagcttaa atatatcgat agagagaata tcaggaagtg atagatacaa
    24301 gacaagttta ttattagctc aaaagttaaa tggtataaaa aatgtatctc aaatagcagt
    24361 tgtaaatgga gtgaaaggat tagctgatgc aattagtgta ggcgctgcag cagcagaaaa
    24421 taacatacca atcatacttg caaatgaaaa gagcgaattg caaggagctg atgaatttct
    24481 aaattcatta aatatagaga aatcttatat tattggagga acagcatccc tatcaaacaa
    24541 tctagaaagt aagttaaaaa atccaactag attatctggt agtagtagag acgaaactaa
    24601 ttcaaagata atagataatt tttataaaaa agatacttta aaaaatgcat ttgtagttaa
    24661 aaatggaatc aagaatcaaa atgatttaat agatggattg gcagttggtc ctttaggagc
    24721 aaaaactggg tcaccagtaa ttttagttgg tgataaatta gctgatagcc aaaaagaagt
    24781 tcttaaaaat aagactttag aaaaagtaac tcaagttggt ggaggagcaa ataaaaatgc
    24841 atttaaagag ttagtaaaat taaaaagtgc taaatagagt acttaagtat taacatatat
    24901 tagtgtgtat tagtaactta tcttaataaa agctgaaaat aattacttca aattattgat
    24961 aaataagata atcaatgatt gtaaagtttt gttttcagct ttaaatttat gtagtataag
    25021 tttaaaaaca taaaaaaggg ttactttata tagaaatata gaaaatctta cattttatat
    25081 ataaataacc catatttaat taacttttta tagtgttcta atagatatta ttgaacttaa
    25141 tggtaaatat aatgtgtaag tttcatttaa agttggatta gattcatcta tatatcttga
    25201 gcaaagtgta attgataagc tatctgcttc tataatttca ccatatagag gacaactgct
    25261 aaagtaagaa tgtacttcaa catatttatt taagttttct tgaacataat cgtaaaaacg
    25321 gttttttgta gtagtagcca taaaatcaac tcctttattt tttattaaag ttgaaacttt
    25381 taaaaagata aatatgatta cttctattag aaactaagtt
//
LOCUS       DAELNY010000042        25142 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.042, whole genome shotgun sequence.
ACCESSION   DAELNY010000042 DAELNY010000000
VERSION     DAELNY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.042"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            4..1860
                     /locus_tag="KPE55_001466"
     CDS             4..1860
                     /locus_tag="KPE55_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0492556.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1943..2183
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2249..2484
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2569..3786
                     /locus_tag="KPE55_001467"
     CDS             2569..3786
                     /locus_tag="KPE55_001467"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0492557.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3903..4385
                     /gene="cdeM"
                     /locus_tag="KPE55_001468"
     CDS             3903..4385
                     /gene="cdeM"
                     /locus_tag="KPE55_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG0492558.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4650..5915
                     /gene="hisD"
                     /locus_tag="KPE55_001469"
     CDS             4650..5915
                     /gene="hisD"
                     /locus_tag="KPE55_001469"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG0492559.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6118..6870)
                     /locus_tag="KPE55_001470"
     CDS             complement(6118..6870)
                     /locus_tag="KPE55_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0492560.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(6988..7257)
                     /locus_tag="KPE55_001471"
     CDS             complement(6988..7257)
                     /locus_tag="KPE55_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492561.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7732..8826
                     /locus_tag="KPE55_001472"
     CDS             7732..8826
                     /locus_tag="KPE55_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0492562.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8828..9778
                     /locus_tag="KPE55_001473"
     CDS             8828..9778
                     /locus_tag="KPE55_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG0492563.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9771..10955
                     /locus_tag="KPE55_001474"
     CDS             9771..10955
                     /locus_tag="KPE55_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492564.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10968..12464
                     /locus_tag="KPE55_001475"
     CDS             10968..12464
                     /locus_tag="KPE55_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0492565.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12487..13437
                     /locus_tag="KPE55_001476"
     CDS             12487..13437
                     /locus_tag="KPE55_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492566.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13458..14420
                     /locus_tag="KPE55_001477"
     CDS             13458..14420
                     /locus_tag="KPE55_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0492567.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14792..15424
                     /locus_tag="KPE55_001478"
     CDS             14792..15424
                     /locus_tag="KPE55_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0492568.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDSLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15835..15927
                     /gene="kdpF"
                     /locus_tag="KPE55_001479"
     CDS             15835..15927
                     /gene="kdpF"
                     /locus_tag="KPE55_001479"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG0492569.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15968..17650
                     /gene="kdpA"
                     /locus_tag="KPE55_001480"
     CDS             15968..17650
                     /gene="kdpA"
                     /locus_tag="KPE55_001480"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG0492570.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17675..19732
                     /gene="kdpB"
                     /locus_tag="KPE55_001481"
     CDS             17675..19732
                     /gene="kdpB"
                     /locus_tag="KPE55_001481"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG0492571.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19751..20377
                     /locus_tag="KPE55_001482"
     CDS             19751..20377
                     /locus_tag="KPE55_001482"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG0492572.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDVRFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20614..21522
                     /gene="cysK"
                     /locus_tag="KPE55_001483"
     CDS             20614..21522
                     /gene="cysK"
                     /locus_tag="KPE55_001483"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG0492573.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21549..22139
                     /gene="cysE"
                     /locus_tag="KPE55_001484"
     CDS             21549..22139
                     /gene="cysE"
                     /locus_tag="KPE55_001484"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG0492574.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22299..22487
                     /locus_tag="KPE55_001485"
     CDS             22299..22487
                     /locus_tag="KPE55_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0492575.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22541..23410)
                     /locus_tag="KPE55_001486"
     CDS             complement(22541..23410)
                     /locus_tag="KPE55_001486"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG0492576.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTKMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23607..24851)
                     /locus_tag="KPE55_001487"
     CDS             complement(23607..24851)
                     /locus_tag="KPE55_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492577.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFNEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9674 a         2683 c         4523 g         8262 t
ORIGIN      
        1 aatatggata cccataataa atatgtaaat tttataaaaa atatacctgt acctttttta
       61 tattgtagaa ttgtaaagag acaagaagat atagaatatc gagtagaata tataagtaag
      121 ggtatgggca aagtattaca attagaagaa ggtatttgtg ataaaaatat attagatgta
      181 ttgcctgtat ttaagtctaa aaaatatttc aaagagttgt tctcaaatga agtggattgt
      241 ataaaaaggt acattccgac acttaaaaac tggataaata taaaaaaaca gataataggg
      301 gattcataca taattttgta ttttggtaaa attgtatttg attatagaca gataatcgat
      361 tcctttgata aaaaggaaaa agtagcttat attaaagacg aagaaggcat atatatagat
      421 tgtagtgaaa atttaatacc aatattaaat aacaacatta aaacaacaaa agatatcttt
      481 ggtaaaaatg atatagaagt atggggagag aatacaggaa aattatttag agatgattat
      541 agagaaggag tatctagtaa aaaaagattt ttacaaaatc tttttgaata tgaagaaaca
      601 ttttttatgg ttgaaaaata ttttttgtat gatgaagacg aacttttagg tactataggg
      661 atagtagaca atataatata tagtggttac agtaatagaa attataactc aaaagattta
      721 atgaagatga tagaacactc aattccagag aatatgtttt ataaagatgt gtatggaaat
      781 tatattggat ttaattcagg ttttttgaat ttagcttgta tgaataaaga agaattatta
      841 gggaagaata gttataaaat atcagaagaa gaagctttaa tagataagat atttgaaagt
      901 gataagggtg tagttgaaaa taaaaaagta gttacatttg agttaaatat aagtatgaat
      961 gatgaaaaca agtgtattga gattacaaaa aggccgtttt ttgatagcta tggaagtgtt
     1021 attggaataa ttggaacagc aagggatata agtagaagaa aaagattaga agaggaaatg
     1081 gataaaacta ggatggaatt ttttgcaaat ttatcccatg aacttagaac gcctataaat
     1141 ttaatatcat cttcattaca agtgatagaa aaaaaagaag cagatttaat agaatcaaat
     1201 gatactttaa aaagaaatct aggtataata aaacaaaatg gaaatagaat attgaggtta
     1261 gtaaataatg ttatagactt tacaaaaatg caatcaggat atttagattt taaaccagaa
     1321 gaatctgata taattgcatt tatagaggag atatgtatgt ctgtagctga ttttgcaagc
     1381 caaaataata tacaacttac atttgataca gaaatagaag aattttcaat gttatttgat
     1441 tcagaaaaat tggaaagaat aatattaaat ttactttcta atggaattaa gtacaataag
     1501 aaagatggaa aaataaatat atttttatat gtgaaagata atgtatttaa tatgaaaata
     1561 tctgatagtg gaataggaat accaaaagaa aaaatagata aaatatttaa taggtttgaa
     1621 cagattgaca atgaactttc ttacagagtt aaaggaagtg gtattggact ttcattagtg
     1681 aaatctttgg ttgagttgca tgagggaagt atatctttaa aaagtcaact tggtattgga
     1741 agtgaattta tagtttcatt gcctgttaga agtaaaaata atattgaaaa atataatcat
     1801 aaaagagaaa tcagcaatga actaagtaaa aaattagaaa tagagttttc ggatttataa
     1861 gtttaaatat aaaaaataaa attttaaaaa agtattgaca attgatttag aagggtttaa
     1921 aataaattac aacataataa ataaaaaggc gatgaatagg aagagtaaat ctaattcgat
     1981 taccagagag aagagtccat tggctggaag actttttaaa ttagagggat tgaaaactac
     2041 ctaggagcta ctttctgaag tcatctgcat tagtagattt tagtaagaat agtcgttagc
     2101 tgcgttaaag tgtaaagtga gttttcatga catatgaaaa ctaaattagg tggaaccgcg
     2161 ggaataatac tctcgtcctt atgttataag gatgagagtt ttttgttatt taaaaatata
     2221 aacagtaatc ataataatta tagaaaatat aaaagcgatg aataggaaga gtaaatttaa
     2281 ttcgactatc agagaggaaa gttcacaggc tgaaagactt tctaagtttg agaaattgaa
     2341 aactacctag gagctgtggt ctgaagttac tttaaaaagt tttagtaaga ctaaccgtta
     2401 actgcgttaa agtgtaaagt ggattttatc aataaatgat aaaatcaaat tgggtggaac
     2461 cgcgagtatg gactcgtccc tttatatagg gatgagtcta tttttattgc aataaaagat
     2521 tgaaaataaa aaaaaatcgg aaaattataa atttaggagg aaaataaaat ggagaataaa
     2581 gtgaaaattg gagtactagg atatggagtt gtaggaagtg gacttataga tataatagat
     2641 aataataaag aaaaaagaaa tatagaaata gttggaattt tagttaataa tttggaaaaa
     2701 cataaggata aaaaatattc taatataata actaataata tagatgatat atttaataaa
     2761 gatatagata tacttgttga agtcatgggt ggtttagaac catcattatc ttatataaaa
     2821 aaagcattaa acaataaaat tcatgttgtt acagcaaata aagatttgtt agctgaatgt
     2881 ggagatgagc ttgcaaaatt agcaagtgaa aataaagtaa gtattaaatt tgaagcttca
     2941 gttgcaggag gtataccagt attaaaacca ataatagaat cactagaagg aaataatata
     3001 gatagtataa atgcaatttt aaatggaact actaatttta tattatctaa aatgtatgat
     3061 gagaatctat cttatgatat ggcattaaga caagcacaag aattaggttt tgctgaagca
     3121 aatccagagt cagatgtatt agggtatgat gctgctagaa aactatcaat attatcaact
     3181 ctagcttatg acaatagagt atattggaaa gatgtgtatt tagaaggaat tacagatata
     3241 gatgaaaaag atattgaata tgcaaaaaaa ttaaattgta aaataaaatt gattggtcaa
     3301 agtaagtatg aaaatgataa agtaagtgct tttgttagac ctgttttagt agaaaaagat
     3361 aatatccttg ctagaataga caatgagttc aatgctgtca tagtgaatgg agattcagta
     3421 ggagaagttt cttttgtagg taaaggagct ggaagtttag caacaggaag tgcggtttat
     3481 tcagatgtta tagatataat agataataga gtttcaagta tagattcttt tactaaagat
     3541 aaaatacaag ttaataaaat agttagagaa aagtgtggag cacttcttag atttaaaaaa
     3601 tgtaataaag atgaaattct aaatatagtt gagaattgtt tagtaaaatt cgatatttta
     3661 aatgatgatg atgaacttgc tattatggtt tatgctgatt ctgaatatga aataaacaac
     3721 agtttatgtc taataaaaga taaaggatac tgcgaaaaaa tgaataaaat gttgaaaata
     3781 agttaaaaaa gaaaaaatat aagtttatat gtaacatatt ttgacgattt taatactata
     3841 tagtatgaga tgaaacaaaa catctcataa aaaaattaaa attaaaggga gagatatgtt
     3901 atatggaaaa taaaaaatgt tattcagaag attggtatga aagaggagaa tctacagcta
     3961 aatggttcca aaatgataga gaagaatatg aaagagaagc atatgatgaa gatagagaaa
     4021 gaagaggttc aaactgtgga tgttcagatt caggagaaaa tagacctaga aactgtgaaa
     4081 gatttagaag agaagctgag ataagagaaa gagaagcaag agaagcattt tgtgaatctt
     4141 cagagaaaaa gaaagaggca ttagcatatg aatgtgaagc tagaaaatta tgggaagaag
     4201 cagaaaaata ctgggatgaa tattcaaaat acaactataa aggaatcgaa tatttagcag
     4261 aagctgctag attatttgat gaaggtatgg aatgtgaagc tagaagaaat ggaaataatg
     4321 gaggaaacaa taataattgt tgccataaat gccataaatg taattgtaac tgctgtagaa
     4381 aataaaaaaa ttaattgaca aatcaatagt taaaatatat catattatgt ataataaaaa
     4441 gaaaattcag aaaaaaataa tgattagaag aaatataagt atatttatta gacaaatagt
     4501 gctcagttag tgaggtgagt acaatatgat taaactccca tcctctagct tttgatagag
     4561 ggtgggagtt tttatttatt gatatatctt aaatgaggat aatattgtaa aataaatgag
     4621 tatgagattg taaaattagg aggtaaaaaa tgaaaagtat taaaacatca agtatgaaag
     4681 aaaaggtaga tgaaaagata ttaagagagc aagtaagttc tataatagaa gatataagaa
     4741 ataataagga tgttgcactt aaaaaatata atgagaagtt tgatagaaat acaagagatg
     4801 aatttagaat tactaaggaa gaaataaagg aagcttataa gcatgtagat gacgaattta
     4861 taaataactt aaagattgct gccaaaaata taagagaatt tgctaaagct caaaaactaa
     4921 gttttgaaaa cccttttgaa aaagaaattt atccaggtgt aatcttaggt caaactaata
     4981 ttccaataga gtcatgtctt gcatatgttc caggaggtca gtatccattg ttttcaacag
     5041 ctcttatgct tataatacct gcaaaagtag caggagtaaa aagaatagtg gcatgttcac
     5101 caactatgaa aaatactgaa aagataaatc ctaagacatt ggtagctatg gatatagcag
     5161 gtgctgatga aatatatgca actggaggag ttcaagctat agcagcattt acatatggaa
     5221 cagaaaagat taatccagtt gacataattg taggaccagg aaataagttt gtaactgaag
     5281 ctaaaagaca atgttatgga caagtaggga ttgattttgt cgctgggcca agtgaagttc
     5341 ttataatagc ggatgaaact tcaaatccag tttatattgc agctgattta ttagcacaat
     5401 gtgagcatga tttaaatgca agaggaatat tattgacaaa tagcttggaa gtagcacaaa
     5461 aagttgaaaa aaatatagaa agtatgctaa aagatttgcc aacaaaggac atagcatata
     5521 gttcatggga aaataatgga gagataatat tggttgatga tatggaagaa gccattaaaa
     5581 taagtaattt ttatgcacca gaacatttag aagttgcagt aaatgagtat aatgatattt
     5641 gtgatagatt aactaactat ggttctttat ttattggaaa tctttctgct gaggtgtttg
     5701 gtgactatgt ttcaggtacc aatcatacat taccaacact taaagcatca agatatactg
     5761 gtggtgtttg ggttggaact tttatcaaaa cttgtacaaa gcagatattt aatgaaaaag
     5821 ctatacaatc attagcacct gtagctgaaa agctggcaaa agaagaggga ttatatgcac
     5881 atgcaaaggc tgctgaggtt aggtttaaaa agtagtaaaa aattttattt tggcagtcga
     5941 gacttagtat tagactgaga taaggaaaac gctgagaacc tgaataaata ctatattttg
     6001 tgggtctcgg cgcttctttt atatctgtat ataatagaat aatactatct gtgtgtttaa
     6061 atactaactt aatatttctt ttctaaataa tgtttatgaa atagttaata tttttagtta
     6121 atacttgaaa aaaacttttg ctaactgtgc aaattcatct gcatttgtca atatagcagg
     6181 gtgtcctccc tgactaaaaa tgtaaatact tgcttttgaa ataaaagttg ccatttgctt
     6241 gtactcttct tcaagattat gacgacacat ttcgtcttcc ttacttcctg taaataaaat
     6301 tggcatttct aatttacaca attctttatg aaacaaaggg cgtttctcgt ttgcacattg
     6361 caatagagct tttgtatcaa gattaacaac attctcccaa tcttttccat gacaccattc
     6421 atagaatttt cgtgcttgaa tatcttgctt tgatttttcc cttccagtga ttagattatc
     6481 tatgaagtta tcattaagag tacgtccatc aaagctatct gctataattg cttcaactaa
     6541 atcgggacgt tctaatccag tattaacagc tgcccatgct cctccacttg ttccaattaa
     6601 gccaacttta ccacaattca aatgttcaat cagtgctatt gtctgtaagg cttcatcatg
     6661 ccatatgtca ggagaaaatt gagaaactct atccgactgt ccattaccta aaaaatcaat
     6721 taagatacat ctaaaatttt ctgcatataa tggcacaagt gctttaaaca tattagaaga
     6781 accagtatta ccatgcagga aaattatcgg tttaccttgt ccatattctt tataaaaaca
     6841 agaattacct tgatactgaa aatatgacat atattttcct cctttttatt tgttaagtta
     6901 attataacaa tataggtatg aatgaaaaac ctcatgttct ttcttagaat aaaaatgtgc
     6961 cacgcataaa acgtgacaca tttctaatta taaattttgc tgtttaagta ttctttgtat
     7021 gcttttctct gtcaaaaagt atttctctga taactgcttg atactcattc cattttggaa
     7081 atttatacag atttgagaat tacgagaagc taagacttgt ttggtgtttg tgttttcacc
     7141 ccaatgcttt ttgtgctctt gctttttggg aatataaatc actttaccat ctatatattc
     7201 ttgcacttgc tcaatcagtt ctttaggcag taagtcaatc gaattttgat agctcatttt
     7261 gcacctccta aataattatt ctttaggatt agcaaaggct actgccactc acaattaaaa
     7321 ataaaatttt ttattatgta caatagcctt tgctatgcac aagttccaat tcccataaac
     7381 taaatactcc tttctggttt gaaaaattca tctgaacatt gatttcgctt ttaagataga
     7441 gttcaatgac aaatttacat ttttattata tatattcaga tagataattg caactgtctt
     7501 tgtatgagga tattttatta tagtcaatac tataatatgt atttatatca tcaaactaat
     7561 atgaacagtt gaaaggaata tgaacaataa aacttagtca ggaagaataa ttatgaagca
     7621 gacaatagag aaatttgatt ttaaagtttt attgaaaatt tattgacaaa tttaacttgt
     7681 aaatattata ctaaacttaa taaataaagt ttataaagta aggtgttgtc tatgaaaaat
     7741 atagttggaa aaccgcaaac gataaaaaaa gtaaatgaag atttaataaa aaacattata
     7801 aaaaatgaag gtcctataac aaaaccagaa atttcaagca ttactgaatt gagtttagct
     7861 acagttaata agattgtaga gcaattagct ttgaaaaatg aagttaaagt aagtggacta
     7921 agtgaatcta ctggaggaag aagagctcaa ttatttgaaa taaatgcaaa cttagaacac
     7981 ataatagctt tatattatta tagaaattgt tgtatagggg tagttgccaa tttattaggt
     8041 gaaataattt atcaagaaga atttaatata agaatggata cgtatgaaaa cgttaaccaa
     8101 gatacttttt ttgttataga tactctaata aaaaaggaaa aaggaaacaa tataagggct
     8161 attggaattg gtgtcccagg agttgtaaaa aaaggtgtag ttaaaaatat accaactata
     8221 aaaagttggg aaggtataaa tttagaaaat ttaataaaga gtaaatacaa tactaaagtg
     8281 tttatagaaa atgatacaaa tattactact attgggattt atcaaaaaca gtataaagac
     8341 aaatataaaa atatggctct tatgtattta gaagatggta ttggttcagg tatagttata
     8401 aatggtgaat tgtatttagg ttctacaaat tttgctggag aattaagtta tctaaatata
     8461 gaatttggta agtataactc taacaactta aatcttgaag attatattat gagtttaaga
     8521 aaaagatatt tagaaagtaa agataataaa tttaagaaag aaattttatc tataatatca
     8581 aactttataa ggactcttgt atgtgtccta aatccacaag ctatgattat acagtgtagt
     8641 atattaacca agagtgacat ggagtttata gaaaaagatg taagcaataa tgttggagaa
     8701 gaaaattttc ctaaattaat aaaagtagac tcattgagag aaggaagtat aaatggagta
     8761 attagtatgt gtttaaaaga aactgactat caatattcct tatctaataa aaaaggagga
     8821 tattaatgtg aaatatttgg tttcaataga tggaggggga acaaaaacta aattttgtgt
     8881 aagtgactta gatggaaaca tacttaaaga acataccact ggttcaacta attataagtc
     8941 agttggaatt aaaaaaacat atgaaaatat taataatgga tttaaaaaaa tattaaaaga
     9001 tttatatatc gattatgacg atattgagta cactgtattt ggtatatctg gttgtgattc
     9061 tcctaatgat tataagatta taatggatga aatattaaaa attgggatta acaaagaaaa
     9121 aatatatctg gcaaatgatg cagttttagc tttttatgca caagctgatt cccctggctt
     9181 agtaattgtg gctgggacag gttcaattat actgggaatt aaagaagatg gagaaatata
     9241 tagagttggt ggatgggggt ataattttag tgatttgggt tctggatatg atatagggag
     9301 aaaattatta aagaaagtat tgctttattg tgatgaatgt catgagtata gtgatttatt
     9361 tagttgtgtc ttggacttct ttggagcaaa tagttttgaa caacttgcat atatgattac
     9421 tgatataaat gataatgttg aaatagcaaa cttagcttca ttagtgattg attgtgccga
     9481 acaaggtgat aaattggcta ttgaaatatt aagagaatca tcaactgaac tatctaaatt
     9541 agctcaagta atattgagaa aaatatctaa tccaatgaaa atggataaaa gcgaaataaa
     9601 tatagttttg tctggtggta cattaaataa aactgtatat gctcaaatgt taatgaataa
     9661 tttaaaaaca gaaaatacag ataaaaattt aaaatttata tctcaagaaa atcagccagt
     9721 atatgggggg ataagacttg ctatggcatt agctaatagg agggtcaagc atggatagaa
     9781 aaaaggtaat agtacaaatt tttactggag gttttaaggc taaagaaata aagtttaatg
     9841 atataaaatg taaactacta gaattattgg actgtattga tatagaaaag gttatcatgg
     9901 gatggagtgt agatgaagaa ttatatgtag aaactaaaaa actattaaat aaatataatg
     9961 ttgaactata tctttggttg ccatgttttt ctgaaatagg attacttgaa aaaagtaatt
    10021 tactaattga ttatatgggg aaggaagtca agggctatgc tttacaagag gatgaaaatt
    10081 ttgagtttta ttgcccaaat aataaggaaa atataaaaaa tataaagaaa atatatagta
    10141 cttattttag tgatattaaa tttgatggaa tttttttaga taaaataagg tatggttctt
    10201 tttcgaataa actaaatggt gtttttaatt gtttttgtaa agattgttta aatatatata
    10261 aaaaaaataa tattgataaa gagtcgttaa tgagagaaat gaataatgtg tctaagggag
    10321 ttatagataa tgaagatact ccatttggta taacagaata cattaatgga aaatacaaat
    10381 ttaaaaataa aatatgggaa gatttttttg aaacaaaaaa tcaaaatata tacaaggcat
    10441 tattagagat aagtagttat tttaaaagta agggattgaa aataggtata gatgcttttt
    10501 ctccttttat ttcttacttt acaggtcaag atttagaaaa attacaagaa gtagcagatt
    10561 ttataaaacc aatgatgtat agaactacaa aagctccagc tggtttacca tttgaatttg
    10621 aaaggtttat tgatgaatcc atacataaga ataaagagat tggaaaaaga aatttcttag
    10681 atattttagg aattaaggag tatgaaggag atgtatatcc tatagaattt gtactagagg
    10741 aaatcgattt tatgacaaga aatcttaata aagatatata tgtaggagtt gaaataaata
    10801 aaaaggaaat tatatctcct gttacaccta agtacataag tgaaaatatt tcaaatataa
    10861 ataagacaac agtaaaagga tatgttttat cttgggattt gttgtcagca ccaaaagaaa
    10921 atattgatga aattataaaa tggtttcgga attagtgagg tgataaagtg agtaagatat
    10981 tggaaataca acaaatatat aaaagttttg gaaaggtaga tgttttaaaa gggataaatt
    11041 ttgagttaga aaagggagaa gtaagagctc ttttgggagc taatggagca ggaaaatcta
    11101 cacttattaa aattttagga ggagttcatt ctcaaactaa aggtaaagta attttgactg
    11161 gtaatgaaat aatgtttaaa aatccacatc attctaaaga aaatggtatt agtataattt
    11221 atcaagaatt aagcttgata ccaacgctta cagtacttga aaatattttt cttggtagag
    11281 aaattttaaa aggaagtgtg tttttaaaca agaaagacat gcttgaggaa tatatgaaaa
    11341 tttgtgagga ttttgatttt aatatagacc cacatatagt agtttctaat ttaagtgtag
    11401 caaaccaaca aatggttgag ataatgaaag ctgtttcatg taattctgaa attatcataa
    11461 tggatgaacc tacaacatca cttactaata atgaaaaaga tgggctattt aaaataataa
    11521 gtagactaaa gtctaaaggt aaaacaatag tctatatttc tcacatatta gaggaagttt
    11581 ttaaagtatg tgatagtgca agtataatga aaaatggaat tatgattgga acttatgatg
    11641 tagaggattt aactaaatta aagataactc aacttatgac aggaaatgat aatcacaaac
    11701 ttattgagaa gaaggattat acatttgcaa aatatgaaga gccaccagta ctagaagtaa
    11761 agaatataag ctggtctaac aaaataaaaa atgtttcatt taaagtttat agaggcgaag
    11821 ttgtaggtct tgcaggtctt gtaggttcaa gaagaactga aattataaat ttgatatttg
    11881 gtatagataa taaagatagt ggagatattt ttatcaatgg taaaaaagta gacataactt
    11941 ctcctaaggt tgctataaaa aacaaaatag gattgattcc tgaagataga aaaaatctag
    12001 gattaatttt agaacaagct atatacacaa actcatcatc agtacaaatt gataaattca
    12061 agaaattttt gtttatagat agaaataaag aaattgagtt ttctaaaaaa ggtgttaagg
    12121 aattaggaat aaaagttagt gatgtaaagc aagaagtaaa agagcttagt ggtggaaatc
    12181 aacaaaaagt agtagtttct aagtggttag agaaagattt agatttaata atatatgatg
    12241 agccgacaaa gggaatagat attgctgcaa aagaagatat tttcaataca atcaaagaat
    12301 tttcaagtaa aggaatagga gtaatattta tatcttctga tttagaagaa gtaataagag
    12361 taagtgatag aattttagta actagagaag gttctattgt agatgattta aaaaatgaga
    12421 atatacaggt tcaagatata atgaacagta tatttaatgt ataaaaaatg aaaatggagg
    12481 actgagatga agaaagcttt tgagaagtta aatttaagag attatggagt attattaggt
    12541 tttttagctt tatgtatagc tataagtatt gcaactccat catttttagg taaacaaaat
    12601 atacttaatc ttttacgaca atcttctata attggaataa tttcagcagg tatgactttt
    12661 gttataatat ctggaaactt tgatatatca gttggagcag tagcagctct tgcaggtgct
    12721 ataacaatga aatttgcgac tggtggaact aatttgtttg catccatgtt cttaggtgta
    12781 gcgatttgtg cagtaatagg attaatcaat ggtatagtag ttgcaaagat aaatgtacca
    12841 tcattaatag ccactatggc tatggtcact attgtaagag gattgctgtt gatgcttaca
    12901 ggaggatatc caattactga aaatatacca atacttgatt acattggaaa tggatatttg
    12961 tttaatatac caataccagt agtaattttc tttatagtgg tgttagcttc gtttattgta
    13021 cttaataaaa ctaagtttgg tagatatgtt tattctgtag gagggaatca agaagcaagt
    13081 aaacttaatg gtataaatgt agattctcac aaagtgaaag tgtttattat taatgctgta
    13141 cttgcaggaa tagctggaat cgtactaaca ggaagacttg ggactgcaac tgcgattgca
    13201 ggtgaaggat atgatatgga tgcaattgca tctgtagtaa taggaggtac atcagtggca
    13261 ggaggttctg gttctgtact taaaactgtt ataggagtat tacttatgag tgttataaac
    13321 aacagtttta atcttcttgg aatagatgta tatttccagt acatatttaa aggattaata
    13381 attttagcag cagttggatt tgattcttat agcaagaaaa aacttgcttc aagataaaaa
    13441 ataaggggga tttttttatg aaaaaattaa tgaaacattt agcattgttg ctatcacttg
    13501 ttatgatatt tggtttagtt ggttgttcaa atggtggtga caaagaaaaa aatggagaaa
    13561 agaaaatagc agtattacta ccgggttcaa caggttactt tgtagcaact aaacaaggta
    13621 tagatgcaaa atctaaagaa ttaggtgtaa gcgtggaata tgcagatgct caatgggatg
    13681 ctagtaaaca attatcacaa gctgaagatt ttatggctaa aggggtagac atgattgcat
    13741 tatgtggagt tgattctgct gtaagtgaga gaatagttaa agctgctaat gattcagacg
    13801 ttcctatagt agcatttaca aattctatag gaagtaaccc aactggagaa tttaaaggtt
    13861 tagttacata tataggacaa aatgaggaag aaacaggagc attaactgga aaaattgcta
    13921 aaaacttatt aggagaaact ggtgggaaag ctgtattaat agaaggagta ccaggaacta
    13981 ctccacaagt attaagaaaa aaaggtcttg aaaaagaatt aaaagatagt aatatagaaa
    14041 tagtttataa tcaaacttca aattgggaga aagagcaagc tttaaaggta acagaagatt
    14101 taattcaaaa gaaaactgat tttaatgtta taatatgtca agatgataat tctgctacag
    14161 gtgcaggtca agctttaaaa gatgctggtc ttaaggataa agttaaagtc ataggtttgg
    14221 gtggaagtaa agatggatta aaagctataa ctgatgggtt aatagatggt acaacttata
    14281 tgtctgctgt agaagaaggt ggattagtca tagaaaaggc atctaaattc cttaaaggtg
    14341 aaaaaataga acctgttaca caaattaaac aagttgaagt taacaaagat aatatttcag
    14401 agttcaaggg tgaatggtaa attgtcatag ataataaata agatttttag agttacttta
    14461 gaaactatat aaagtaactc tttttttaat ttaatttagt gataaattat tgaatagtat
    14521 ttctaaacaa ataaaaaatt aatataaaca aagacttaat tatactaaat tataagtaaa
    14581 actaatataa acttatggaa aaaaggaaaa cgtctaaagt attggaatta ctatagtgta
    14641 ggtgtggtat taaaaaaaca taaaaaaaca taaaaaaatt gttgaaaaaa atatagaaat
    14701 tttgtataat taaagatttg aaaagtgcgg tatacaaagc caattctttt gtatataata
    14761 tatataaatt cagaaaaagg aagggatatg tatggccagg cggttatttt tagaaaggat
    14821 tggacttata tttttaggtt cagcaatatt agcatttgga gtttataatt tttattattt
    14881 aaacaacata acagaaggtg gagtgttagg tattttacta ctgttaaaaa atttattcaa
    14941 tatacaacca gcaatagcaa atgttgtaat agatagttta ctacttttag taggatataa
    15001 atttttcggt aaaaaattcc ttatatattc aatagtagct tctataactt tttcagtgtt
    15061 atatgactta tttgaagcta taggacctct agtaccacaa tcacaaaata tgcttttatc
    15121 aactattttg gcaggtgtaa ctgttggaac tggtgtaggt atagtagtaa aagcaggttg
    15181 tgcatcagga ggagacgatg ctttagcatt agtaatttct aagactacat ctttgaacat
    15241 agggcaagtt tatttagcaa cagatgttat tgtattgttg ttatcattat tttatctatc
    15301 agcgtttgat atcttttatt ctttgattgc tgttacaatt agtggcaagg taattgattt
    15361 tatttattat catggtaaaa gtttggatat ggatataagt aatgatatag ttccagaatg
    15421 ttaatataaa ttaaataact ttagatttta ttaggggtgg cagatttgcc accttttttt
    15481 tgtctaaatc tttatagaaa gtataatccc atctaggttt ttgcaaatct caaatataga
    15541 attatgttgg aatgactggg aatacgattg ctagatgtgc attttaaaaa cccaatttta
    15601 tatttatgaa atactaatca ttaatggatg tttcttaatc ctatctttat acctaaaatg
    15661 ataagataaa tgaagtagca atacaaagct aaaatttgta aaagagatta aattaaatgg
    15721 aggagctaaa atgttggata tagttatgat agcattgtta ttcataggat ttgtttccat
    15781 gaagttactt gctgattggt gtgggaaaca aactgaaaaa aaataatgta atgtatggag
    15841 ggattaccta tgtggtttct ggcagttgta attataatgt taataatata tttggtatat
    15901 gcgcttatta atcctgaaaa attttaaaca gtataaggct aataagtaag ttgaattggg
    15961 gggaacaatt tttatggtgc agatagtaat agtgttagcg atttttatga ttttagtaat
    16021 acctatgggc aagtatttat atcatatagc tactaatcaa aaaacatttg gagatagact
    16081 atttgataaa gtggacaatt ttatatataa ggtatgtagt atagataaga aaaaagaaat
    16141 gaactggaaa cagtatgcat tagctttatt atttacaaat gcagttatgg tatttatagg
    16201 atatatcata cttaggactc aaagtatgca tatttttaat ccaagtggaa ttaaaagtat
    16261 ggaacaagga ctatcattta atacaataat aagttttatg acaaatacaa atcttcaaca
    16321 ttattcaggt gaatcaggac tttcatactt tagtcagatg acagtgataa tatatatgat
    16381 gtttacatca gctgcaacag gatatgcggc tgctatggca tttgtaagag gtctagtcgg
    16441 taaaaagaaa acattgggaa atttttatgt agatttaatt aggataacaa caagagtact
    16501 tttaccagga gctttaatta ttgggcttat attagttaca caaggagtac cacaaacttt
    16561 tgctgggaca gaaacagtga ctactataga aggaaagctt caagatatag ctagaggtcc
    16621 tgtggcagct cttgaatcaa taaaacattt aggaacaaat ggtggtggat tctttggttc
    16681 aaattcatca cacccatttg aaaatccaac aataatatca aatatagtag aaatattatc
    16741 tatgatgata ttaccaggag cttgtgttgt tgcattcggt cacatgataa agaataaaaa
    16801 acaaggttgg gttgtgtttg gcgcaatgtc aatcatattt ttaataggtc tagtagtgtg
    16861 ctttaaagca gaaagtgcag gaaatcctat tttatcacaa ttagggctta atcaaagtat
    16921 gggaagtatg gaaggtaagg aagttagatt tggtatagca caatcatcat tattcacaac
    16981 agtgactact tcctttacga caggtacagt taataatatg catgatacac tgactccttt
    17041 aggaggatta gtaccacttt taaatatgat gctaaatgta gtatttggtg gtaaaggcgt
    17101 aggtctgatg aatatgttga tgtacgctat aatagcagta ttcttatgtg gattaatggt
    17161 aggaagaaca ccagagtttt taactaagaa aatcgaagga aaagaaatga agcttattgc
    17221 tttacttatt atattacatc cacttttaat actaatgttc tctggtcttt cagtagcaat
    17281 accagcagga cttgaaggaa tttcaaatcc tggattccat ggattatcac aggtattgta
    17341 tgagtttgca tcttcagcag ccaataatgg ttcaggattt gaaggtctag gagataacac
    17401 tatgttttgg aatataacaa caggtatagt tatgttcttt ggaagatatg tgtctattat
    17461 agtactacta gcaatatcaa gtttgttagc atctaaaaaa gctgtaaatg aaagtatagg
    17521 aacacttaga acggataatt ttacatttac aatagtttta gtacttgttg ttttaattgt
    17581 tggtgcttta acatttttcc cagccttagc gcttggacct atttcggagc atctagtgtt
    17641 gtggcactaa aaaattggga gggagtaaag ttttatggaa actaaaaaaa caaagtttat
    17701 tacaaaagat attttcaaaa cttccattat agaagctttt aaaaaattaa atcctaaata
    17761 tatgatgaaa aatccagtta tgtttgttgt agaaattgga tttatagtaa cattgatact
    17821 tacaatagta cctagtttgt ttggtgatga aggcaataac ttaagagctt ataatgggat
    17881 tgtaacaata atcttattta taacagtcct ttttgcaaat tttgctgaag ctgtagctga
    17941 aggaagaggt aaagctcaag cagatacact taagaaaact agaaaagata cagtagcaag
    18001 ggttatagat acgttaggaa atgaaaaaat gataagtgca agtgagctaa aacttggtga
    18061 tattgtttta gtaaatgctg gagaagttat cccaaatgat ggagaagtta tagagggtat
    18121 agcttctgtg gatgagtcag caataactgg agaatctgca cctgtcatga gagaatcagg
    18181 tggagatttc gcatctgtta caggtggtac tacagttgta agtgactggt taaagataag
    18241 aataacaaca aaaccaggag aatcgttctt agataaaatg atagctcttg tagaaggtgc
    18301 ttcaagacaa aaaacgccaa atgagattgc tttaaatact ttgttagtag ggttaacact
    18361 tatatttatg gttgttttaa tatctcttta tccaatggca aactatgcag gtgtaaagat
    18421 tccaatttct actatgatag ctcttttggt atgtttaata ccaactacta taggtggatt
    18481 gttgtcagct attggtattg caggtatgga tagagtaact agatttaatg taattgcaat
    18541 gtcaggaaaa gcagtagaag cttgtggaga cgttgatact atgattttgg ataagacagg
    18601 tacaattact tatgggaata gacttgcatc agaatttata gcagttggaa atgcagattc
    18661 aaaagattta ataaattatt ctgttatgtg ttcattaaaa gatgatacac cagaaggaaa
    18721 atctatagtt gatttaggta tgaaattagg ttcaacaggt aagacaaaag aggcagagga
    18781 agctgagttt gtagaatttt ctgcacaaac tagaatgagt ggtattaact tagctgatgg
    18841 tagagctata agaaaaggtg cttatgatag cataataaaa agagtaaaag aatctggtgg
    18901 tacagtgcca gaagatttag aagaaaatgt aaatcgagta gctaaactag gagggacacc
    18961 tttagtagtg tgtgtagata atgagatata tggaattatc tacttaaaag atacagtaaa
    19021 atcagggctt gtagaaagat ttgctagact tcgagagata ggaataaaga caataatgtg
    19081 tacaggggat aatcccttga cagcagctac aattgcaaga gaagctggag tagatggatt
    19141 tattgcagaa tgtaaaccag aagataaaat agatgcaata aaaaaagaac aattagaggg
    19201 taaaatagtt gcaatgacag gtgatggaac aaatgatgct cctgcacttg ctcaggcaga
    19261 tgtaggtctt gcaatgaata gtggtacaac atctgcaaaa gaagcagcaa atatggtaga
    19321 tttagattca gacccaacta agatattgga agttgttgaa ataggaaaac agcttttaat
    19381 aactcgtggg gctttgacaa catttagtat agcaaatgat gttgcaaaat attttgcgat
    19441 tataccagct atatttactc ttgcaatacc tcaaatggag atattaaatg taatgaaact
    19501 ttctacatca tttagtgcga tattatcagc tttaatattt aatgctataa ttatcccatg
    19561 tttaatacct cttgcaatga agggagtaaa atatagacct atgaaatcac aagctatgtt
    19621 aatgagaaat atgcttatat ttggtcttgg tggagttata gtaccatttt taggaataaa
    19681 attaattgat ttattgatta cacctttagt aatggttctt aatttaggtt aataaatgga
    19741 ggatataaaa atgaaaacat taaagccagc attacttgta agtattgtat tgttagttgt
    19801 ttgtggactt gtatatccac ttgttttgac aggtgtaagc caagttgcat ttaaagataa
    19861 agccaatgga agtatgatag aagtaaatgg tgtaaaagta ggttcagaac ttattggtca
    19921 aagctttaca gatgtgagat tctttaaggg aagagtatca tcagtaaact ataatactta
    19981 tacaaaagaa gatttagtac cagataaaga tggaaacaca tcttatggag gtgtatcatc
    20041 agggtctttt aattatggag ctacaaatcc agaacttcat gatagagtac aaaaagatat
    20101 agaacagttt ttaaaggata atccaactgt caaaagagag gatataccaa cagatttact
    20161 tacagcatca ggttcaggac ttgaccctaa tataagtcca gcctctgcaa aaattcagat
    20221 accagctata gcaaaagcgt ctggaatatc agaaagtaaa ttacaaaaaa ttgtagatga
    20281 taatacttcc aaaaaacttt ttggagtttt aggtgaagat agagttaatg tattaaaggt
    20341 taatgtagag gtcgcaaaaa tattaggtct tatatagtat tttgtggaat tttagcaaca
    20401 aaatattacg caagatttaa tttaaaggtt aaattttaaa gagtgaaaat cgataatgat
    20461 tttcactttt ttatttgaat attactaata tttggaataa agtgattgta agtataaaat
    20521 ccaaaaaaag gttgcattat caaaatgata atactataat atatttatcg ttatcaattt
    20581 gataatagat ttggacatat aggaggaaat agtatgttat ataataacgc attagagttg
    20641 ataggaaaca cacctgtagt aaggctgaat aatttaggct gtactaatgg gtgttctaac
    20701 atatatgtaa aattagaaaa agtaaatcca gcaggaagta taaaagatag agctgtttat
    20761 gaaatgttag agggcttaga gcaaagagga gaattaaaaa agggagatgt actagttgaa
    20821 gcaactagtg gaaatactgg tattgcatta tctatgatag gaaaattaaa aggatatgaa
    20881 gtagtaatcg taatgcccga aacaatgagc gttgagagaa gagatttaat gaaggcttat
    20941 ggagcaaatt tggtgcttac agatggtaaa gctggaatgg caggttccat agaaaaagca
    21001 aatgaacttt taaaagaaaa tccaaactat aaaagcctaa aacaatttga aaataaagat
    21061 aatccaaatg cacattataa aacaacagca gttgaaatta tgaatgatgt caaagattta
    21121 gatatattcg tatgtggagt tggaacaggt ggtactttaa taggaactgc aagatattta
    21181 aaagaacaaa atccaaatat aagagtaatt gcacttgaac caaaaaaatc tccagctata
    21241 tcagaaaaca agactggacc tcataaaata caaggaatag gtgctaactt tgttcctgaa
    21301 aattatgatt ctagtgtagt tgatgaagta atattagtag atgatgaaga tgcatttgat
    21361 acagttaaat tacttgcagc aaaagaggga attttagtag ggatatcttc tggagcaaat
    21421 atatttggtg ctatagagat ggcaaaaaag tatccagata aaaagatagt tacagtagct
    21481 ccagatggag ttgataagta tatgtctatg ggaatatttt aataatttta tttgagggag
    21541 tgaatacttt gtttaagaaa ataaataaag atattgaata tataatgaag aatgatccag
    21601 cagctaggtc taagatagaa gtttttttac tttatccatc agttcatgca atgataatgc
    21661 ataggatggc tcatgctctt tataagaaga agaagctttt tacagcaaga ttaatatccc
    21721 agatatccag atttatgact ggcatagaaa tacatcctgg tgctaaaatg ggggaaggta
    21781 tattgataga ccatggaatg ggtgttgtaa ttggagagac agcggaagta gggaataggg
    21841 tgacaatata tcaaggagct actttaggag ctacaggaaa agatactgga aagagacatc
    21901 caactgttgg tgatgatgta ttaattggtg ctggaacaaa aatactagga cctttaaata
    21961 taggttcaaa ttcaaaaatt ggtgcaaatt ctgttgtcgt taaggatgta cctaatggtg
    22021 caacagttgt tggtatacca gcaaaaatag taaaaataag aaacctagaa cctgtaaaaa
    22081 agaataaaaa agaagtgtcc tatgagtatg atgaattaga taatgtatat tatatatagg
    22141 atttatataa ctataaaata aactttataa aaattatttt aaaaggtatt tttttcaaat
    22201 ttgaggacat aataaaaaag aactgaatgt tacagttttg gttcatcaat ctttgataaa
    22261 caagatttta aatttgtagc gtctaggagg attttattat gaaagcaaat gttaatcaag
    22321 atacttgtat aggttgtgga ttatgtccat caatatgtcc agaagtattt gatatgaagg
    22381 atgatgggaa atcacatgtt atagttgatg aagtaccaag tgatgctgaa gaatcagcag
    22441 cagaagctag agaaagctgt ccagttgatg caatagatgt gcattaataa aataaatgcc
    22501 tataaaaatt gaattttcaa tttttatagg catttgtctt ttattcagtt agtatttcaa
    22561 cacctgtttc tgtaactaaa accatacatt cccattgagc agataattta ccatcttttg
    22621 tataaatagt ccaattattt tcttcatcaa caaaaatttc gtgagttcct tcatttacca
    22681 taggttctat tgtgaatatc atcccaggaa ccaatagcat cttagtccct tttttagata
    22741 cataacttac aaaagggtct tcatgaaagc ttaatccaac tccatgacca cctatttctc
    22801 taacaacaga gtatccattt ttctttgcat aagagttgat agcatcacct atatctccta
    22861 aaaatcccca tggtttggca acttcaagac ctttttggac acattccttt gtaacttgaa
    22921 ctaatttttt tgcattttca ttaacattac ctatcataaa cattcttgaa gcatctgaat
    22981 agtatccatt aaaaatagta gaaacatcta catttataat atctccatct tttaagataa
    23041 tgtccttact tggaatacca tgacaaactt cattatttac tgatgtacat acacttttag
    23101 gaaatccatt atagtctagt ggagcaggga tagctccatt ttgtagtgta aagtcatgaa
    23161 ccaacttatt tatatcttct gtagacattc caatttcgat tttatctgaa atataatcca
    23221 aaatttttgt gtttattaca gcactttttt ttatttcctc aatttgactt tcatttttta
    23281 taatatcatg agttggtaca atatgaccta acttagcaaa tgaatctatt ttattatcca
    23341 tatccatatg acatttttta tattttttag tacttccaca ccaacagcta tcatttctat
    23401 ttaacttcat aacttttcat ccttctttta ttatttattt aaattatggg ttacataaac
    23461 tattttaaaa cataaccatt ataaatatta taacataaat actaagaaat cctttataga
    23521 tagtgattta ttcaaagtat aaattattat atggttattg ttgatgaaaa gaggatagtt
    23581 aaatgtatta ttggatatta gcgcacttaa tagtttaatt caagtttttc aagaaaatca
    23641 tatgttagag gacaattttc ctttaaagta ttttttgaat cttcatcaag ataataatgc
    23701 acaaatgatt ctgcaaaaaa ttcttctata aacttgctta tatatgcatg tgcaacttgc
    23761 ttagggtcat aaagcttata tctttcctga gcaaatactt ccataaattc ttcagaattt
    23821 gattttttat ttaaaacatt tttatctata gcatgtgcag tttcatgtaa ttctaaattt
    23881 tttgatgaat gtacatttgc atagctaggg ttactataac ctattttagc cactatagaa
    23941 ttatttctat atataccagg tacatctttc caagtcttgc ctgatcttac ccaacatgga
    24001 ggtaattgat attttaaatc actaaattct ggttcatcag ttagatttga atttattaat
    24061 tttattttta tatccttttc tttaagtgtc tgtaaatatt taatatttat ttttgcaagt
    24121 ctgtttttca tattttcaac ttcatcttta tcataattca agtcatcagg aagtataagc
    24181 atttgttcaa ttatatctat actactgtct tttatatttt cagattcttc attaaattta
    24241 tattgaagag tattattttc atagatagtt atatttgtat tattttctat agaataagag
    24301 tgaatatcct ctaaatctaa aatattcatt tgttgtaaaa tatgttttgt ttcgccactc
    24361 catactacta aagaaagtga attaaaatta attagtggat tttcttttgc ttttatatca
    24421 ttgcttatat ctacatcaaa tacatattca attgagtctt ctatgttaag actatctgta
    24481 ttctttttaa tatataaata cgcttgtttt aagtctatat aaatctcatc taaagattcg
    24541 tcataaaatt ctgataaatt attagtaaag agttcttcac ttaaaagttc atttttatca
    24601 gttatacttt caattaaaat attaatttct tttatcatat cgtaaatact tatttttcta
    24661 tttgcttttt cactagatac attgcatggc gcaagagcaa tgcaataaaa atctttttct
    24721 tttttataaa aggctttaag tgattttgat aaaagtgtaa agtctacacc tatctgtaag
    24781 ttgaccttaa gtgagtcatt ttgtaataat tcagtattat ttcttttagc tgtatttaaa
    24841 ttgttttcca taatcattgc ccccttttta ttagggtatc ataatttttt tatatgtgtc
    24901 aatttgtttt tttgaaaatt ataatttttt tgaaaatttt attttttata atttgtgaat
    24961 tttttattat agaaataaat atgttattat tatgtcaaaa tttagcatta gattcatgta
    25021 gagtatgttt tatacatata ttataattaa ttctttgaag attgcatatt caaaatacac
    25081 accatttttt ttaattctat aatataatat ttatatattt aatgttagga tgtgatttaa
    25141 gg
//
LOCUS       DAELNY010000043        24429 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.043, whole genome shotgun sequence.
ACCESSION   DAELNY010000043 DAELNY010000000
VERSION     DAELNY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.043"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="KPE55_001488"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="KPE55_001488"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="KPE55_001489"
     CDS             275..466
                     /locus_tag="KPE55_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG0492578.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="KPE55_001490"
     CDS             621..2030
                     /locus_tag="KPE55_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0492579.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="KPE55_001491"
     CDS             2161..2850
                     /locus_tag="KPE55_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG0492580.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="KPE55_001492"
     CDS             2853..3788
                     /locus_tag="KPE55_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG0492581.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="KPE55_001493"
     CDS             3785..4678
                     /locus_tag="KPE55_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG0492582.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="KPE55_001494"
     CDS             4756..5502
                     /locus_tag="KPE55_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG0492583.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="KPE55_001495"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="KPE55_001495"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG0492584.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="KPE55_001496"
     CDS             6359..7063
                     /locus_tag="KPE55_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG0492585.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="KPE55_001497"
     CDS             complement(7251..7679)
                     /locus_tag="KPE55_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0492586.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="KPE55_001498"
     CDS             complement(7716..9146)
                     /locus_tag="KPE55_001498"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG0492587.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="KPE55_001499"
     CDS             9278..9862
                     /locus_tag="KPE55_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG0492588.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="KPE55_001500"
     CDS             9878..10399
                     /locus_tag="KPE55_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG0492589.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="KPE55_001501"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="KPE55_001501"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG0492590.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="KPE55_001502"
     CDS             12762..13532
                     /locus_tag="KPE55_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG0492591.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13869..14300
                     /gene="phnG"
                     /locus_tag="KPE55_001503"
     CDS             13869..14300
                     /gene="phnG"
                     /locus_tag="KPE55_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG0492592.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14315..14890
                     /gene="phnH"
                     /locus_tag="KPE55_001504"
     CDS             14315..14890
                     /gene="phnH"
                     /locus_tag="KPE55_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG0492593.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14893..15978
                     /locus_tag="KPE55_001505"
     CDS             14893..15978
                     /locus_tag="KPE55_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG0492594.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15998..16867
                     /locus_tag="KPE55_001506"
     CDS             15998..16867
                     /locus_tag="KPE55_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG0492595.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16860..17726
                     /locus_tag="KPE55_001507"
     CDS             16860..17726
                     /locus_tag="KPE55_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0492596.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17767..18480
                     /locus_tag="KPE55_001508"
     CDS             17767..18480
                     /locus_tag="KPE55_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0492597.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18481..19650
                     /locus_tag="KPE55_001509"
     CDS             18481..19650
                     /locus_tag="KPE55_001509"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG0492598.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19654..20490
                     /locus_tag="KPE55_001510"
     CDS             19654..20490
                     /locus_tag="KPE55_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0492599.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20491..21486
                     /locus_tag="KPE55_001511"
     CDS             20491..21486
                     /locus_tag="KPE55_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0492600.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21504..23192
                     /locus_tag="KPE55_001512"
     CDS             21504..23192
                     /locus_tag="KPE55_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0492601.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23204..24271
                     /locus_tag="KPE55_001513"
     CDS             23204..24271
                     /locus_tag="KPE55_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0492602.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9742 a         2741 c         4368 g         7578 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggtcttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagcgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaagtaag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggatacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gaattttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc tagctcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttact ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtagt ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg gttaaaagca gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aaaggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactaatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaactaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcga ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgt
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gtacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttctcaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gtttatgagg
    13621 cactcttttt gtatgaaatc aaatatattt tcatcatttc aacaatgaaa aagaagttta
    13681 tcatattttg agatagtttg tttttattta tttaattcat attttaaaat atattttctt
    13741 aacattgtta aaaaagaatt tacaagatat tcattagaat taactcaact ctaatattca
    13801 cttaacttaa gttgtttatt atattattaa agagaagtaa acgaaaagta gattaaggag
    13861 gttgaatttt gtatacagtt gatagtacta agatattgat aaatatgaac ttagagaaac
    13921 tagaaaagat atactcaatg ataaaagata catcaagtat ttctataatt aaagaaccta
    13981 acttggctac agtaatggta agagctaatg aaagtgtaaa aaacacaaca tttaatttag
    14041 gagagatatt agttactgaa tgtagtgtaa aggtagatga aagcttgggt tacggaatag
    14101 tttcagaaaa caataataag aaagccattt atttagcagt tatagatgca gttttacata
    14161 gcaataacag taaatttgat gagttaaaga attacataaa taaaagtgta tatgaagaga
    14221 acttaaggta tgaacaagag attattgatg aatttagctt gattaataaa acgaaagttc
    14281 aattcaatgc tatggattaa ataggaggag aatcatggta aattatacac aagaaatata
    14341 tagaaagatg ctagatagtg cttcatttcc tgggaaatta aataatatag ataatataaa
    14401 tatagaaaat aatacaaaac tattgaatgg agcaattggt atagcaatca ctttattaga
    14461 ccaagaagtt accttctata tagaaaatta tactcaaaaa gatataaaaa atataagagc
    14521 acttacaatg tcaaatcaaa tagattatag aaaatcagat tatatatttt tagatattaa
    14581 ttcagaagtg gatatattac agattaaaat aggttctttt gaatatcctg atgaaagtgc
    14641 aactatagta catcaagtaa atgatttatc aacgtactct caaactaagt atataaagct
    14701 tgatttgagt ggtcctggta taaaatctaa aaatagtata tatataaatg gaattagaga
    14761 agaatttata gaaagattat ctagcataaa taaagattat ccaataggaa tagatttgat
    14821 tttagtagat aaaaaaggag aaattgcatt tataccaagg tcatcaaaat taagttggga
    14881 ggtattatag aaatggctta tgtagcagtt aaaggtggag aagaagccat attacaggct
    14941 aaaaatatac ttgagtttta tagggtaaaa ggagatagct tgcctataga tgtaaagcag
    15001 attgaggaac aaatgtctta tgctgtagac aaagtaatgt ctgaaggaag tttatacaac
    15061 aaggaactat cagcattggc tataaaacaa gctcaaggtg atatattaga agcctctttc
    15121 tatgtaagag ctttaaggtc aacaatgcct agaattacat attcagaacc cataaataca
    15181 gaagaaatga gaataaaaag aagaatatca gctactttta aagatatacc aggaggtcag
    15241 tatcttgggg caactagaga ctatgaaaat agaatattga atgtagaatt attagaagaa
    15301 gatgaaaaaa agaatagaca ttttagagat agttttataa atgaattaga aacagtagat
    15361 gaaatccctg aatttacaaa aatatctaat atatttagaa aagaaaattt attacaagaa
    15421 gaagtttgtg aaaacaaatt ggaagaagtt attgatgata ttacaagaaa tagtttaaga
    15481 tttccagttt ctaggtcagc aaagttacag gcattaacta gaggtgaaag tggagtagtg
    15541 tcaggttttg cttatgctat tgtaagaggt tttggtgatg aacatccata tataagtgaa
    15601 cttagaactg gtaatgtatt tataaaagta aaacatccca tctatgaaga tgaagaaata
    15661 ataatagggg atatgctggt tactgaatgt gaaatagttt caaaagcatc acaaaataag
    15721 actgaaaatt ctaaaactaa ttcggaagat aaaaataaga tgtctttagg atatggtctt
    15781 tgtatgggga ataatgagaa taaggctata tctatggcca tactagacaa atctttggag
    15841 ccaaatagtg aaaaactagc acaagatgaa gaatttgtat tgcttcatat agatggaata
    15901 gattcattag gatttatatc tcactttaag cttcctcact atgtagattt taaagcggat
    15961 gtagaaagaa taaaatagtt tgaggaggta agtttaggtg atatataact atggtttttt
    16021 agacgaaaac acaaaaaaag aaataagaag aaagatatta aaagcaatat caatacctgg
    16081 ttatcaagtt ccctttggtt caagagagtt gcctatagct aagggatggg gtactggagg
    16141 tttacaatta acactatcat taattggtaa aaatgatgta gtaaaagtaa ttgaccaagg
    16201 aagtgatgat tctacaaatg cttgtaatat aaggagcttt attgcaagtg taagtaatgt
    16261 agaaaccaca aaagatacat tagaagctac attgattcaa acgagacata gaataccaga
    16321 agaaaaactt aaatctaatc aaatactaat atttcaagta ccaataccag aaacccttag
    16381 aatagtagaa cctagtgaag tagaaacaag aagaatgcat tcagaagaag attatagtag
    16441 gatgtgggta tatctttatg aagatatagt tagatttaat gatatatcta tagcagttga
    16501 atatccatgt aaagtaaatg atagatattt aatgaatcca tcacctatac ctagatttga
    16561 tataaaaaaa ttaaatatgt cagacaattt attcttgttt ggtgcaggta gagaaaaaag
    16621 aatttatgct attcctccat atacaaaggt agaaccttta gagtttgagg attataagtt
    16681 tgaagaggaa aagtttgaag gaaaatactg tagtttatgt aacagtacaa atacattttt
    16741 agatgaagta tatgacagtg acactaatga aaagtactat agttgctcag atacaagtta
    16801 ttgtgaaaaa gttagattaa aaaataacag tatagatgtg acaataggag gtacttggaa
    16861 tgagtaaaat aaatgactct aaaagattag aaaatataga caagcaagta ttaataggaa
    16921 gaaatatcaa tcttatttat ggagatgggt gtgaaaagtg ttttgaaagc acaggatatg
    16981 aaagcaataa tatatgtaag tattgtggtt ctatagttgc atgtaataat gtaaatttag
    17041 aactttatga aggagaagtc cttggaattg taggtgaatc tggttcagga aaaagcactt
    17101 tattaaaaat actgtttttc caagaacatg caaatagtgg agaagtatat atatcccatt
    17161 acaataaaga cacaaatata ctagagcttt cagaccaaaa aaagagatac ataaagaatc
    17221 attttatggg tattgtgtat caaaatcccc accttggttt aaatttagac tttagtagtg
    17281 gtggtaatat agcagaaaaa ttattgatgg caaatttata taatgttgaa aaaataagag
    17341 atagaggcaa agaactttta ataaaaacta atatacctgt agaaagaata gaccataaac
    17401 caaaatactt tagtggggga atgcaacaac gagttcaaat agcaaaagct ctttcaaata
    17461 accctccaat attgctatta gatgaggtta caacaggttt agatgtttct gtgcaagcgg
    17521 aagtattgga tttaataaga gaaatccaaa gagaacttaa gatttctatg atagttgtat
    17581 cccatgactt tgatgttata aagatgcttg ctgataggac aattgtaatg aaaaatggaa
    17641 gtgtaattga gtctggtctt acagaccaaa taatggagga cccacaacac ccatatactc
    17701 agcaacttat aaattcattg ctataaatat cgtttgtaaa taagcaaaat gggggttttt
    17761 aataaaatga aagaaactat acttgaagta aataatttta gtaaagactt tgagttacat
    17821 gcattgaaca agactataac ggcatgtagt aatataagtt ttgaagtttc aaaaggtgag
    17881 tttttaggaa taataggtaa atctggagca ggtaagtcta ccatattaaa aagtatctat
    17941 aaaacatata taccaacaac tggagaaata atttttaatt cagaaatata tggtaatgta
    18001 gacctggcta aaattggaga ccgagaaatg ataaatctta gaaagaaaga aataggatat
    18061 gtatcacagt ttttaaagac acttccacga ataactgcaa ttgaactggt agtacattct
    18121 ttaattgaga gtgggtttga aaaagattgt tcgtatgata tggcaaaaga tatacttaac
    18181 caatttgaaa taaaggaaaa tttatgggat gcgtatccaa ataatttttc tggtggagaa
    18241 aaattaagat taaatttagc tcaagctatg gttaaaaagc ctagactttt gttacttgat
    18301 gaaccaactg catcacttga taatcaatct aaaatatatg taaaagaaaa aatgttggag
    18361 cttaaaagtc aaggaactac aatgatagga atttttcatg atatagagtt tatggaaact
    18421 gttatagata agacctttac tatgacaaaa gggactataa gtgaaagtgg ggtagcctag
    18481 atgataaaac taattcataa tgcaaatatt gtattagaag atatgatttt agaaaatggt
    18541 catttaatca tagagggaga tacaataaaa aaagtatcaa atgataagat tgattacgca
    18601 tttgacttta atgagataga tgaaataata gatgcaaaaa acttatatgt aatacctggt
    18661 attatagata ttcacagtga tgccattgaa aaggaaatag agccgagacc aagtacatta
    18721 ttaccattta atatggcatt ttatgaacta gataaaaaac tacctgcaaa tggaataact
    18781 acagtttatc attctatatc tttaggggat ggagtaggag ttagaagtat tgataactct
    18841 ttaaagatga ttaaaaatat tgattcatat aaaaatatag atagtaagag cataaatcat
    18901 aaagttcatt taagatatga agtgttgtat tatgaaggtt tagaaaaggt tttagaacta
    18961 ttagatgaaa ataaaataga ctacttatca ataatggacc attctccagg acaaggtcag
    19021 tatacaaatc caacatttta taaagagtat gctactaaag tgtggggagt tacagaaaat
    19081 tatgtagata catggttaga tgatttagta aatctgcatg ataatttaga ttggaataaa
    19141 atagctaata ttataggtat agccaaaact aaaaatataa atgttgcatc acatgatgat
    19201 gatacattag aaaaaatgga tttcataaaa aatataggca taaatgtatc tgaatttcct
    19261 ataactcttg aagttgcaaa acattcaaag aaattgggtg ttctaacttg tttaggtgca
    19321 ccgaatattg taagaggtaa atctcataat aacaatttaa aagcaatgga tgctatattg
    19381 gaagattgtt gtgacattgt gtgttcggat tatcttccat cagctatgat aaagtctatg
    19441 tgtatagttg cagaaagaat aaataattta aataaagcag tcagtttatt tacctcaaat
    19501 cctgctaaag cagttggtat atatgatgaa agaggtagta taaaagaaaa taaaaaagca
    19561 gatttagttt tagtagatat tgatagtgaa tatccaaagg ttgtaaatac tattgtaaat
    19621 ggaaaaacag tttataaaag ggaggtataa agattgctaa cagagttaca ttgtcataca
    19681 agtatatcag ataataattt tactactgaa gaaataataa aaaaagcagc agaaaagaat
    19741 ataaatatct tagcaataac aaatcatgac acattatctg gattggatga agctcttgtg
    19801 cttggagagc attatggaat aacaataata ccaggtatag aaatttcagc ctatgattat
    19861 aaaaatagaa aaagagttca tattcttgga tataacatag atttaaacag tcaagaaata
    19921 aaaaatttgt gcaatccaat ggtatgtgac agacataaag catctgttaa gatggtaaat
    19981 aaaattatag atttaggata caaaatatct ctggaagatg taaagaaata ttcatcaaag
    20041 actggaatat ttaaacaaca tataatgaaa gctcttatag ataaaggata tacagataat
    20101 atatattcaa atctatataa agaattgttt catagaggaa acggcaaggt atatgtatct
    20161 ttaaaatatg tggattacag agatgcaata aaagctataa aaaattctgg tggaatttgt
    20221 gtgttagctc acccgggtca aatggataat tttagcgcta ttgaagagat ggtaaaagta
    20281 ggattagatg gaatagaagt atatcatcca tctcacaaca aagaactaga aaaagagtcg
    20341 ctttcttatg ctaagaagta taatctagtg gtcacaggag gttcagatta ccacggattt
    20401 tattctcaac ataattatga acttggaagc aagagcttaa ataaagaaga tttatttaag
    20461 tttaggttag ctttaggagg ggtattataa atgtcttttt tacaagtaaa aaatgtaggt
    20521 aaaagttatg gacaagttaa ggtgttaaaa gacatatcta ttgatattga aaaaggagag
    20581 tttatttgtt tactaggtcc aagtggttgt ggaaagagta cactacttag aataattgca
    20641 gggcttgagg ataaacatgg tggaaaaata ataataaatg ataaggatat gactaattca
    20701 ccacctgaaa gtagaaattt tggtatagtt tttcaatcat atgcattatt tccaaatatg
    20761 aatgtttaca aaaacatagc ctttggacta gaaaataaaa acataagtaa atctaatatt
    20821 gataaaaaag ttaaagaagt tctagaagtt gtagaattaa gtggatatga gaaaaaatat
    20881 ccatcacaat tatcaggtgg acaacaacaa agggttgcat tagctcgtgc gattgcttta
    20941 gagccagact ttttacttct tgatgaacct ttatctgcat tagatgcaaa agtaagactt
    21001 aaacttaggg agcagattag aagtttacat agaaaactag gcattacaac tataatggta
    21061 acgcatgacc aagaagaagc tctttgttta gctgataaga tggttgttat gaatagagga
    21121 gaaataatac aagttggaac gccaaaggag gtatataaaa atccagaaac accttttgta
    21181 gctgatttta taggaacaat aaattttata gatgatggta ttaataagat agctattaga
    21241 ccagaagata taaaagtaga gtctaataga gattctaaag ataaggatat aaaagtggga
    21301 gaaattttag atatcgaatt tagaggattt aactatagaa ttacagttga gtatcgttct
    21361 aagcaaatga aattagatgt agtttccaaa gttgcagaac aaatgaaact gtgcataggt
    21421 tctaaaataa actttaagat acctaaagaa ggaattgtac aatataaatc agagggatgt
    21481 gcttaatagc taggggtgaa taaatgataa catcaaaaaa taaaagaaga ataagtgata
    21541 gcacaataca aaaatatttc ttgatttttt cggtaattgt actaatagta tttatattat
    21601 ttccattagt atcattatta aaaaatgcat ttatggatac aagtggaaat tttataggaa
    21661 tttcaaactt tagtaaatat gttgaaaatc caagtcttat agcatcattt aaaaattcaa
    21721 tttttgtatc tacagtctca tctttaatat cactatgttt agcatttata tattcatacg
    21781 caataacaag aacaaacata aagtttaaag aattttttaa aactatggga atgtttccac
    21841 tttttgcacc aactatgtta tatggtattt cacttatata cctttttgga aataagggaa
    21901 tctttacaac catgggtctg gaagtaccac tatatggtcc attaggaata attatttctc
    21961 agattctatt tacatttcca caagcttttt taatattatc tgtagcactt tcgatggctg
    22021 attacagact ttatgaagca gcagatagct tgggtgctag tgaatttaag aaattttgca
    22081 aaataacaat tcctggtata aagtactcat tacttagttc attctttgta tcctttatac
    22141 ttgcatttac agattttgga gcagctaagg tagttggagg aaactacaat gtactggcta
    22201 ctgacatata taaacaagta gttggacaat ttaacatacc aatgggagct actgtttcta
    22261 tggtaatgtt aattcctgta ctaatagcat ttacattaga taagatggca agtaagaaac
    22321 aaggtatgac cataactgca aagtctatgc catataagat taaggaaaat aagtttagag
    22381 atagagtatt tttaatagta tgtagtttta taacattgct aatttttatt ttaatattcg
    22441 tttcagttat aggttcagtt ataaagttat ggccatataa tttaagcttt acaatggaac
    22501 actatacatt taaaggtatt ttgggaagtg gaataaaaac atatttaagc agtttgaaaa
    22561 tatcaacttt aacagcaata tttggaacta tatttgtatt ttttagtgct tatatgatag
    22621 aaaaggtgga taagtttcct aaattaagac aaacagctta ccttttatca atgataccaa
    22681 tggccttacc tggcttagtt ttaggtatat catatataac tttttttaac tcagcaagta
    22741 atccattaaa ttttttatat ggaagtacag caatattagt tatagtaaat gtagttcatt
    22801 tttattcagt ttgttttata acctcaaatt catctttaaa aatgttggat aaagagtatg
    22861 aattagttgc aaaatccata aatattccat tctataaagt attttttaat ataacagttc
    22921 caatgtctat aacatcaata cttgaaatag tagtatatta ttttgtaaat tcaatggtta
    22981 ctgtttcagc attaatattt ttatatacac ctcaaactca gactgcatca atatcaatac
    23041 taaagttaga tgagatagga tatataggac catcagctgc tatggcagta ttagtgcttt
    23101 taacaaatat aatagtaaga cttttgtacg aatttgtaac aaagaaatta aaaaataaca
    23161 cacaaaaatg gcagcaaaaa gatgttgcat aggggaggta aaaatgaaat ttaagaagct
    23221 agtttcttta ggagtattat caacactttt attaagtgta ataacaggat gtagtactga
    23281 ttcatcaggt tcagattcaa agtctgatag taagacaaaa ggggatttaa cagtttacac
    23341 agcaattgaa gaagatagta tagagccata tttagctaca tttaaagaga aatatccaga
    23401 tattaaatta aatattgtta gggcatcaac aggagatata acagcaagat tattagcaga
    23461 aaaggataat cctcaagcag atgttgtatg gggagttgca gccacaagct tattggttgc
    23521 agatgaccag gggatgttag aaccatatgc tccaaaaggt tctgaagaaa tagagtctgc
    23581 atttaaagat gataaagaag ttccttcttg ggttgggata gatgcttgga tgacaggtat
    23641 aacagtaaac actaaagaat taagtgataa gaacatccca gttcctagtt cttacgaaga
    23701 tttaataaag ccagaatata aaggtcttat atctatgcct aatccatctt cttctggtac
    23761 agggtactta acagtatcag cccttataca aattatgggg gaagaaaaag catggcagta
    23821 tatggataag ttacatgaga atattggtgt atacactcaa tctggttcag ctccagctac
    23881 atcagcagct tcaggagaat atcctattgg tatatctttt ggatatagag gtataaagtt
    23941 aaaagaggaa ggctatccag tagaagtagt atttccaaaa gaaggttctg gatgggatat
    24001 agaagcaaat gctcttgtga aaaaagataa tataaaagaa gcatcaaaag tatttttaga
    24061 ttgggctata agtaaagatg caatgaacga atactctaaa aactatgcag ttactactat
    24121 ttctacagga aatccaatac cagaaggatt ccctaaaaag ccattagagc aaatgataga
    24181 taatgattta aaatcagcag caaaaaatag agaagatata ctaaataaat ggatatcaaa
    24241 atatgatggt aagacagaaa aagagagtta agtaaaacta tacaaatgga gtaaaaagaa
    24301 aaattttttt taagtagatt ttattattat aatataagtt gagaataata taggtcaaga
    24361 aaagtgaata ttctataaaa atgtatagag taagtatacc attcagtttg aatggtatat
    24421 tttttattt
//
LOCUS       DAELNY010000044        23920 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.044, whole genome shotgun sequence.
ACCESSION   DAELNY010000044 DAELNY010000000
VERSION     DAELNY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.044"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..1648
                     /locus_tag="KPE55_001514"
     CDS             98..1648
                     /locus_tag="KPE55_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0492603.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            1674..4376
                     /locus_tag="KPE55_001515"
     CDS             1674..4376
                     /locus_tag="KPE55_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0492604.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDIKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(4882..5547)
                     /locus_tag="KPE55_001516"
     CDS             complement(4882..5547)
                     /locus_tag="KPE55_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492605.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFSAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            5891..7321
                     /locus_tag="KPE55_001517"
     CDS             5891..7321
                     /locus_tag="KPE55_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0492606.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEICLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            7689..8267
                     /locus_tag="KPE55_001518"
     CDS             7689..8267
                     /locus_tag="KPE55_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0492607.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            8297..8443
                     /locus_tag="KPE55_001519"
     CDS             8297..8443
                     /locus_tag="KPE55_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0492608.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            8748..10127
                     /gene="scfB"
                     /locus_tag="KPE55_001520"
     CDS             8748..10127
                     /gene="scfB"
                     /locus_tag="KPE55_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG0492609.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            10156..10653
                     /locus_tag="KPE55_001521"
     CDS             10156..10653
                     /locus_tag="KPE55_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG0492610.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            10951..12615
                     /locus_tag="KPE55_001522"
     CDS             10951..12615
                     /locus_tag="KPE55_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG0492611.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVIAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            12628..15078
                     /gene="recJ"
                     /locus_tag="KPE55_001523"
     CDS             12628..15078
                     /gene="recJ"
                     /locus_tag="KPE55_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG0492612.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            15091..15603
                     /locus_tag="KPE55_001524"
     CDS             15091..15603
                     /locus_tag="KPE55_001524"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG0492613.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            15677..17884
                     /locus_tag="KPE55_001525"
     CDS             15677..17884
                     /locus_tag="KPE55_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG0492614.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            17909..18358
                     /locus_tag="KPE55_001526"
     CDS             17909..18358
                     /locus_tag="KPE55_001526"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG0492615.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            18373..18987
                     /locus_tag="KPE55_001527"
     CDS             18373..18987
                     /locus_tag="KPE55_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0492616.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            19078..20634
                     /locus_tag="KPE55_001528"
     CDS             19078..20634
                     /locus_tag="KPE55_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG0492617.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            20624..21886
                     /locus_tag="KPE55_001529"
     CDS             20624..21886
                     /locus_tag="KPE55_001529"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG0492618.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            21960..23747
                     /gene="aspS"
                     /locus_tag="KPE55_001530"
     CDS             21960..23747
                     /gene="aspS"
                     /locus_tag="KPE55_001530"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG0492619.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         9598 a         2366 c         4304 g         7652 t
ORIGIN      
        1 atttaacgtt ttacttgaaa ttatgtgttt tgtaatgtat tattaaggtt ctaataggaa
       61 cattttcatc aatttttcta tagtgggggt aggaattatg aggtacaaaa aaagaaatat
      121 agataagtca agtgaacttt catttcaatc tctagcaaaa tattttataa ctttctatat
      181 agtaattata ttaattattc tttctatgtt ttgtataaat acaatatggg ttatgaaaaa
      241 agataatcaa gatttattat ctacaacgtc aaatcgtatt gaagataggg taggagaaac
      301 aattaagcta ttgaactctt tgagtaaaga taattacata atagataata gtgtgaatgg
      361 catagataaa agtaaagaac tttcaatgta tagtgatgaa tttgggtata tgatgatatg
      421 ttttgttgat aagaatataa ttgtatggga tgaagaaggt ggtaatatta gtctagctgg
      481 tcgtgattat atgcagaaac tttttgcaac aaaaaaatat caagttacag atagttttgc
      541 agcaggggct gatggaaaaa ctttaaacta cactattgca tcacctataa taaagaatgg
      601 aaatgtagat ggttgtgtat ttgcagcaat ttactttgat gagataaagg atatactcaa
      661 acaaggaaaa ggtcaacagc aacagaattt catattattt ggaagtgaaa atcaaataat
      721 gaatttaaca agtgatgaaa ctgcatatgg agagaagtat atgaagttat cgcagaaaac
      781 aaaatatctt ggaaccaatg caaaaaaagt agaaaaggat atgtttaata ataaatcagg
      841 tgattattgg gcttttgaaa atggaaaatt atactttatg tcctataaac caattaagaa
      901 cacaaattgg agtttatttt gtaaagttaa tttcttagat atttttgcat taaatttaat
      961 tcctatggga gctattctat ctgttataac tttagtattt attattttga tttgtatgga
     1021 gaaaagcttc atgaaaaaac gaatggaagt tattgatatg cttttaggtt ctgttcaaga
     1081 attagaaaaa cgtatatatc aaacagaaag acctagtgaa gtagacttta aagaaattat
     1141 tcagctcaca agtaaaggtc tgactgatgg attaactggt gttgtaacac gtactgtatt
     1201 ttttgaccaa atttataatc gtgttgaaat ggaggaaaat gataaattat ctgtaatttc
     1261 atttgttgat ttagatgatt tgaaatgtat taacgactta tatggacatg aaattgggga
     1321 tatggttttg aaaaatgttg gatatgtact tcgtgaatat gagataaaat ataatggttt
     1381 aattgggcgc tatggtggag atgaatttgt tatgttgtta tctggtttta catcgagaga
     1441 agaggtggag gatttattgg atgagattgc tattcgctta catactgaag tactaacaga
     1501 tgatggtatc attcaaactc attgtagtgt tggagctgta gtatgtagtg taaaagacaa
     1561 taaagatata gataatttat taaaacaggc tgatgaagct ttatactatg ttaaacgtaa
     1621 tggaaaaggt aattatcatt tagtttaatg tatgtggaag gagtttatat catatgaaaa
     1681 atgtatctaa aaaatttttt gtatgtacta ttattgtatt aataacattt ttatgtttta
     1741 gtaaatttgc tatttatgtt tttgacaaac aagaaaagaa agaaacaaaa gtagtagaaa
     1801 acatgttaaa tgtctacaag aataacatta ttttaaatct gactgaaaaa tttaattata
     1861 gcaaagactt gacatcagtt ttagtttcaa acaattattc tatagataaa ttaaaaccta
     1921 ttgcagatga aattcttaaa aacaattctt gcatatatat ttcagttgta gaaaaatata
     1981 atgtagctgc atttttttct aattctgtca aaaaagatat tatcaatact ccaatacagg
     2041 aactgaatta ttcttattca ttagcaaatt ctatcaaagg attagtcgta gaaggtcctt
     2101 ctaaagacca gttttcaaat ggagatgtgt ttttaattac taatcctatt tataaaaatg
     2161 gagagtactg gggagaaatt acagtagctt tagatgccaa aaaagttgta gattctttta
     2221 acttaaataa attaaaagaa tctggatttg agtatgaact ttggcgtgta catctagata
     2281 atggttcaaa atctacaata gaaatttcta ctaaagattt taatttttcc aaatcacatg
     2341 aaattaagtt taaacttccg actgtttgga ctcttagcat tgtgtacaaa aatggatgga
     2401 ataataagaa tatagatttt attgtatttg ttatattgtt aattatttcc atattaatag
     2461 gtgtgatgac atttatttac attgaaaaga agtcgattag taaggaatta aatcaacaga
     2521 gtcttaaaaa tttagaaact ggtattttga atagagaagg ttttttaatt aagttacaag
     2581 agcatctgaa tagaaaaaat aatgaaatag tgttgctgta tatatgtatt actaattata
     2641 gtaaatttgc tccagttatg agctttgaaa aaagacaaga ttatcttaca catatagtaa
     2701 aaaatctaga taattacata aaaagttctc attttattgc tcgtataagt gaggaaggtt
     2761 ttgttctagc tttagaagaa aattgtgata aaaatgaatt gcttaacatt gaaaaaggga
     2821 tttccttaga gcttttacga aaaattaaag tagaaaataa acaagtattt ttaacgattg
     2881 gttatggctc atctaggtgt gttgatgata gaaaagatgc agaatctctg ttactttcag
     2941 ctattgaaga ttatgagaga agatttccag caatgtctcc tattcatgat tttactaaaa
     3001 agtgtgaaga tatgcttgat gaaaagaaac agattgtatt tggtgaatat ccagatttag
     3061 aaataaacaa gttgtctaaa gtaatataca aatattataa aaaagtacag cgtattatgt
     3121 ataaagatta taatattgat attggaaata gacgagggta tatgcgtgat gtcgaagtat
     3181 taattgatta taattctgag agaatgttat acttattttt aatagacata tgcaagtttg
     3241 gaaaatttaa tgaactgttt tctattgaaa taggagatgt tattttaaaa gaagttagta
     3301 acaaattaaa aacaattttt tctaattatc tatatcgtat taatggagat gtatttttag
     3361 gcatttcaat agataaagaa tcttacttag atactttaca taaggttaat gaagtattta
     3421 atagttcttt aagtgtagaa gatgtacttt ttgatataaa atgtaatatt ggtatttgtc
     3481 aatatccata tcatggtgaa aatccatatg accttctgga aaaggtacaa attgcattgg
     3541 agtatgcaaa aaaggatgtc aaaagtaatg ttaaagttta tgataaggaa ttgacagaag
     3601 agatttacca tgaaaataat attattaagg atttaaaaaa aggtctggaa gaacaaacac
     3661 tagaagtttg gtatcagcca atttttaatg ttagtaaaaa ctgttatata aaagctgagg
     3721 cactaattag attgagaaat aaagagggcg gttattattc tgcctatgaa gttattactt
     3781 atgcagaaaa gcatggactt attaaggatg taggtctata tgtactaaaa aaggcttgtg
     3841 atgtaactac aaaaataaaa gataaaaata tagattttga acaaatgcaa ttaaatttat
     3901 ctgttcagca gattgtacat aagcagtgtg caaatgacat attatcatgt attgagaatt
     3961 gtggtctaga taaaaagtat atttgtgttg aagttacaga aacagtactt atggaaactt
     4021 ttgaaattgt aaatgttact cttgataaac taaaaaaaga aggcattagc atagctttag
     4081 atgagtttgg ttctggattt agtagtataa attatctggc aaatttacct attgaaaatt
     4141 taaaaataga taaggaatta gtagaacaaa ttacctattc taaaaaacaa tttgaattat
     4201 taaaaactat agttcaaatt gctaagatta atggcatgga tattgtagca gaaggtgttg
     4261 aaacaaaaga aactctggat ttagttgtaa cttcaggtgt gaatttgatt caaggtcatt
     4321 atttttcaga acctttaagt gaagataaat ttattgaact tatgattgaa gaataaaggt
     4381 taataaaata aagatttaag gtattgaaaa ataattaaaa gtatttaata atagaattag
     4441 ggaagaataa gtttataagt aaaaataatt gattagtagt taataaaata aatgtgatat
     4501 ccaaataaat cctaaatcta atttgagttt taatatagat agtctaaagt ttgattatta
     4561 aagatttaat tgtaagttgt tacagttgat gatattgatt agagattaaa gtttagagta
     4621 gaaagttacc ttaaatttaa aaatgcaaga taacccaatc taaaaattat aatcactcga
     4681 taggtttaag gatttatttg tttattataa ataggagggt gtctcaaaat gaacttttta
     4741 gtttatgagg cactctcttt tatatagaat taaatatatt ttattatttc aacaatgaaa
     4801 aagaagctta tctctatttt gagacagcct ctttgtatac tattattaat ttaatcaata
     4861 acataattta aaattatatt attagttaag atttcctgct ttattagtaa ataattcttt
     4921 aatagcattg tttctggaaa ctaaatcgtt atcagagttg tttgtttttt tcataatcac
     4981 tgaataacgt tttttattta taaaaaatat taaaatctta tcatcatcaa aattgtattc
     5041 tttatatctg tcaatagact cataacctag atttagaatg tttttgtctt ttatactatc
     5101 tacatctaaa atatatgatg ggtcagcctt tagtaatata ttgattaaac atttttcttc
     5161 accagcagag aataactcca tcttacttga tatagattta gattgctctg aagttatttt
     5221 tgcaggaata aggttgtcat tatcatctct tagaagataa ccatgtgtgt cttgttgata
     5281 tactaaatta acctttttct taggaacact aatcatttta tctaaatgta cataatgatg
     5341 attttcaaca aaaccttcat ttaatatttc aacaaagtta ttaattttac ctaggcaaaa
     5401 agattctata gattctttac agaatttatt tgtaaatggt attatgttta cgttttcctc
     5461 gtctgtattc catgctatag cataatccaa cgaatatact tcttcctcta tatctgcaaa
     5521 attatattgt actatgtttc ctttcaaaat tactcgacct ccatcttata atatatttaa
     5581 tatactttat aaaacttata ttatatttaa ataatatcat aaattggata aatatgatat
     5641 agtaaataag aaaaaatttg aaaaaaatca ctataaatga gtaataaata taaatagtgt
     5701 atactaatgg gatggaaaat gtccactatt aactttatta ccccacatat ataagataaa
     5761 attattaata taaaatataa tctatcgaca atgaatagac ttttaaataa acttaatata
     5821 aaattaatta aataagtgct taaaaacgat ttactttaat gatagaatca tattaaagga
     5881 ggataatttt atggatataa agaataaaat taaatctata tttttaccag aaaaaaatga
     5941 ctatttagat gatgaagaat ttgagtatga tatagaaaat aatgaagaag atttaaagga
     6001 agataacaaa gtacatttaa atgatttaag tagagtaaaa tatgattaca gagagttaaa
     6061 agacatagaa aaccaaacag aagagatatg tctgctagca gtaaagcagg atggtactat
     6121 aattgagttt gtaaaggaca agacttataa agtatgtatg gaagcagtaa aacagacata
     6181 taaatctctt aagtatataa caaatcaaaa tgaggatatt tgtatagaag cagtaaaaca
     6241 gaactacaga gctttgtact acataaataa taagacagaa aatgtactaa ttgaggctat
     6301 aaaaaatgca tcaacttatg atgtcatgga agtatttaaa tttgtggaag agcaaacaga
     6361 agatgtatgt ctagcattta tagaaagggc atctaaaaat gatgttgcag agatattaaa
     6421 aggaataaaa gagcaaactc cagccatatg tttagaagct gtaaaaaaag atggaaaatc
     6481 tttagcttat gtaaaggaac aaagtaatag tatatgttta gaagctgtaa aagaaaacta
     6541 cagtgcacta tcttgtgtaa aagagcaaac tgaagaaata tgtatagaag cagtaaaaca
     6601 gaatgacttt gcattgtatt atgtaaatga acagacagaa aaaatatgta tggaagcagt
     6661 aaaaagaagt tatatggctc tacagtatgt aaataagcaa acagaagaga tttgtttgga
     6721 agcagtaaga attgatggaa gagcattaca gtacgtaaaa gagcaaacag aagaaatttg
     6781 cttggagtca gttagacaaa atggaaaagt actgcaatat gtaaaaaaac agactgaaaa
     6841 tatatgtata gaagcagtaa gaggttcatt tgaagaactt gaaataaaag aaatattaag
     6901 ttatgtaaag ataccaacag aaagaatatt tgtagaagca gtaaagcaaa atggaaaaat
     6961 tcttaaatat gtagaaaatc agacagaatt aatatgttta gaggcagtaa gagaaaacta
     7021 taatgcttta gcttatgtaa aggaacaaac agaaaagatt tgtttagaag cagttaatca
     7081 aagttatgaa gcgcttaaat atgtgaaaga acaaacagaa gaagtttgtc taaaagcagt
     7141 aaaacaagat tatagaatgt tgaaatatgt aaataatcaa acagaaaaaa tatgtttaga
     7201 agcagtaaag caaaattata gagctttaga atttgttgat aatcaaacag aaaaagtttg
     7261 tcttgaagca gtaaaacaga atagaaaagc actacagtat gtaaaacaaa aacaaagtta
     7321 aaaggctaaa atatatagaa aatatatgca aataaataaa atttgatatt ttgtaataaa
     7381 taaaatgggt atctcaaaag gaacttttta gtttgtgagg tactcttttt atatgaaata
     7441 aaatatattt ttagactttt aacaatgggg aaaaggttta tttctatttt gacataggct
     7501 ttttatgcat ataaagcttg ataaaattgt tttattataa ttgttttgtt atataaataa
     7561 taagaagaaa attatatagg tctttttttg accagtttta catttttaac aactagtttt
     7621 gtaaagtata ttgaattttc tcaaaaaaga atgtactcta tatatgtaaa atttttttgg
     7681 aggtaaaaat gtttaaaaga tatgctgaaa aaatgacatc tgttttaatt tgtaataata
     7741 tgatagataa taatgaatct aaagtctatt cttatggatt tgaaatatta atagctttca
     7801 tagttaatat aaccaccatg ctgtttatag gttttttatt tggtaaattt acatatgtat
     7861 tatttttttt aatgtgttat tgtccaataa gacaattttc aggagggtat catgcagaca
     7921 attattttag atgcttatta acctttattt ttataatttt atccacaatc ttaattatag
     7981 aaaatataaa tatagactta tttaaaaata ttattatgat tattgcaagt gttagttggg
     8041 ttggaatttg tgttctgtgc ccaatagaac ataggagtaa ccctataagt gatagggaaa
     8101 agttggttta taagaaaact gctatattta tatctacagt agtgttattg ataacaattt
     8161 taagtctttc tttgagtata tttgtagatt atttcacata tagcgcattt gccatgtttt
     8221 ggatttttgt gatgttagta ttaggtaagt taaaagctaa agtataagta aaatatgtat
     8281 gagggagagt taagttatga aaaagtttat tgtaagattt atgaagtttg ctagctcatt
     8341 ggcacttagc actgctattt tgtcagctaa tagtacctgt ccttggatta ttcatcagcc
     8401 taaagtaccc aaagaaatca gcaatctaaa gaagacaaat taaatacaag cacttattta
     8461 tacaaaatta tcaggaaaaa agaaattatt atattttaat aagattaaaa caatataaaa
     8521 tcagatacaa aatttatttt atttatttta taggagccta atacctattt ggtaatatat
     8581 tagtgcgcca atttaggggg atatagtgta tttaatgaaa tgacttacgg agagtaagtc
     8641 atttttgtat tgtatgtatt ttatgtaaac ttatgatata atgaagggta aagtttaatt
     8701 aaaataataa aaaactttag tatagatagt aatttaggag gtagagtatg agtatgatac
     8761 ataagttttc tatgaatgga tataatatcg ttttagatgt gaatggtgga gctgttcatg
     8821 tgttagatga tgttgcatac gatttgttag atttttataa agaaaaaagt aaagaagaaa
     8881 tattagagat attaaaatca aaatatcaag aagaaaaaat aaatgaagca tatgaagaaa
     8941 tattaaattt ggaaaaagag ggtcttttat atacagaaga tacatatcaa tatcatccaa
     9001 gctttgtaca tagagagcct gtagtaaaag ctttatgttt aaatgtagct catgattgta
     9061 acttaaaatg taaatactgt tttgcagctc aaggtgattt tggaggagaa aaagaactta
     9121 tgagttttga agttggtaag gcagcaattg actatttgat tgctaattca ggaagtagaa
     9181 agaacctaga gatagatttc tttggtggag agcctttgat gaactttgaa gttgtaaaac
     9241 aactggttga ctatggaaga agtgtagaaa aagattataa taaaaatata agatttacaa
     9301 taactacaaa tggtgtactg ttaaatgatg agattataga ttatataaat gaaaatatgc
     9361 ataatgtagt tttaagttta gatggaagaa aagaagttaa tgataatatg agaccaactt
     9421 taaatgataa aggaagttat gatattacat taccacgatt taagaaactt gtggaaaaaa
     9481 gagcaaaaga taagtattat tatataagag gtacttttac aagggataat ttagattttt
     9541 caaaagatgt tatgcatttt gcagatttag gatttaaatt aacctctgta gagccagtcg
     9601 ttggagatga gagtaatcca tatgcactta gagaagaaga tttacctaag atatttgaag
     9661 agtacgaaaa atttgctgta gagtatgcag atagacaatt acaaggagat ggatttaaat
     9721 ttttccattt tatgatagac ctaaatcaag gtccatgtgt aataaagaga ataactggat
     9781 gtggagctgg aaatgagtat ctttctgtaa ctccaaatgg agatatctat ccatgtcatc
     9841 aatttgttgg aaatgaagaa tttaaaatgg caaatatatt tgatgaagaa atagtacttc
     9901 cagaaaattt aaaaaatatg tttagagaag ctcatgtata tactaaagaa gagtgtaaac
     9961 aatgttggaa taagttttac tgttcaggtg gatgtcatgc aaatgcgata aactttaata
    10021 atgacatatc aaaaccatat gaattaggct gtgaaatgca aagaaaacgt acagagtgct
    10081 ctataatgat acaagctaag ttaatgttag aaggagcaac caactaaatt tatattttaa
    10141 aggagttgtt tatatatgat tagagattat ttagaagata aacctttaat tgacgaaagt
    10201 gtatttgtgg caaaatcagc agatgtaatt ggaaatgtta aaataggcaa agattcaagt
    10261 atatggtaca atgcagtagt taggggtgat gaaggaccaa taactattgg agaaaacact
    10321 aatatacaag attgttctat agtgcatgga gatacagaaa ctataattgg taacaatgta
    10381 acagttgggc ataggtctat agttcatggt tgtaaaatta gtgataatgt gttaattggt
    10441 atgggttcaa taatattgga taatgctgaa ataggagaat atactctgat aggagcaggt
    10501 acactaatta catctaataa gaagtttcca ccaggtgttt tgataatggg ttctcctggt
    10561 aaggttgtta gagagcttac agaagaagat aaaaaatata ttgatgaatc ttatgaatgg
    10621 tatttagagg cagcacaaaa tcaaaaatat tagtttaaag agtggttgtt tcaaaacata
    10681 tttaaatctg ttttgaggca accactaatt tattttatta gattttgttg atattttagt
    10741 tttcaaaaag tatttgatta aaccagatat aaaatgtcaa aaataaaaat taactgagtt
    10801 aaataatttt aataaaattg gtagtatatg tgataaaatt aaatattaaa gaggatatag
    10861 gatattagaa tatcaattgg tgagataatg tcatatacga taaattaagg tgtaagtttt
    10921 tgggtatacc tatcttagga gtgaaaagat ttgagaataa gttatagaaa tttaaagcga
    10981 tatagagaaa ttggatatgt tttaataaag tatggtttta gttttattgt agaaagacta
    11041 aatatagaag gaattgccta taaaatacca ctatttgacc caccagaaga aataaaaaat
    11101 atgacaactg gtgagagaat gaaaagagtt ttagaagaac taggacctac atatataaaa
    11161 ataggacaga tattgagtac aagaaaagac ttgttagacc aggatataat tgatgaaata
    11221 tcaaaactca gagatgatgt agagaaattt gattcaaata tagctattga tatatttaaa
    11281 gaagaagtgg gtttaagtat agaagaaata ttcttagaat ttaaagaaga accaatagca
    11341 gcagcttcaa ttggtcaagt ttatgaaggt gtacttaaaa cgggagaaga agtaatcgta
    11401 aaaattcaaa gaccaaatat agagaaaata ataaaatctg atttagagat tcttagaact
    11461 attgcaaata ctttaaaaga tttaaaaaag gattttaatt tagatttagt acagatgatt
    11521 gaagaatttc aaactcaatt aatgagggaa cttgattata catttgaagc tataaatgca
    11581 actaaattct ctaggatatt taaaaatagt gatgaagtat atatacctaa agtatattca
    11641 gaatataata ctaagaaaat tttagtaatg gaaaaagtta atggtacaaa gttaagtgat
    11701 gtagaaaaaa taaggagact aggttataac acaaaaacta ttgttgaaat aggcgttagg
    11761 tcatttttta cacaggtatt atctcatgga ttttttcatg cagacccaca tccaggtaat
    11821 atattcgtaa ttgctaaaaa taagatagca tatatagatt ttggaatgat tggaattatt
    11881 gataataaaa ctttaaacca attaaatgaa attgcattag caggtgtaga aaagaatgtt
    11941 gacaagataa tatatctatt gatagaaatg gatgcattaa atggagaggc tgatattaag
    12001 ggacttagac aggatttact ttatctaata cattattact atgatatttc tatagaaaaa
    12061 ataaatgtaa cagatatatt aaatgaacta tttaggtttt ttagacagta taaaatagtc
    12121 atgcctgcac aatttgtaac tcttgcaaag acagttataa cattagaagg aacaagtaga
    12181 actttaaaca cagatttttc ttttggttct atgggaaaag agtttatgaa acatcattat
    12241 aagagtaaat ttaaccctaa aaatgttgtt ttaagttcaa gacaaaatgt agaagaaata
    12301 ttactggaca taaaaactat tccaaagcaa ttaaaagcaa ttttaaaaaa tatagaaaga
    12361 aataatataa aaatgcaaat tgaagatgtt aaaatgacga ggttagaaaa ttgtataata
    12421 gagctaacat ctcaaatatc tttgagttta gtgttggcat ctattatagt aggttcttct
    12481 cttataattg catctccaaa tatagaaaat aatatatgga taaaatttac tgcaatagcg
    12541 ggtttcttta tatcctttat aataggcctt tgcttagtta taagaagtat taggtcaaaa
    12601 tacaaaaaag attaggggga attaaatttg aaaaaatgga tattgaagta taaaggtaag
    12661 atagaggaaa gtgaatttag caaaaaactt aatatttccc cggaaatctg ccaaatttta
    12721 aaaaatagag ggatatgtac tgaaaaagat tcagagatat ttatgaatcc atctttagat
    12781 tatttaagag accctttttt aatgaaagat atgcaaaaag ctgttgatag aataaagtta
    12841 gcagttgaga agaacgaaaa aatttggatt tacggagatt atgatgttga tggtgtatca
    12901 tctacatcta tcttgtgctt atattttaag agtataggtt acaatgttga ttactatatt
    12961 cctaatagat tagaagaagg gtatggtata aatgaagatg ctattaagct tataagctct
    13021 aggggatgtg acttgattat aagtgttgac tgtggaatta catcagtgtc tgaggtcaat
    13081 acagcgaatg atttgggcat agatgttata attacagacc atcatgaatg tcaaagtgaa
    13141 attccatcag cctttgcagt aataaatcca aaacaagaag attgtaatta tccatttgat
    13201 tcactatgtg gatgtggtgt agcatttaaa atgattcaag ccttgactcc aaaagaagaa
    13261 tttaaaacta gtatgtataa ttatttggaa atagtaacac tagcaactat ttgtgatatt
    13321 gttccactta tagatgaaaa tagaataatt gtaaaaaatg gtttaaaatc aatgaaagaa
    13381 ggcaaaaata ttggattaag ggagcttata aaagtttgtg gtgtagaaag tgataagata
    13441 ggttcttctc atataggttt tgcaataggt cctaggataa atgcttcagg tcgattggga
    13501 tattcttact taggagtaga gctatttact actcaatcac aagaagaggc agtagaaata
    13561 gctagtatat tggaagaaaa aaataatgaa agacagatga tagaagctaa gatgtatcat
    13621 gaagctgagg aaatgctaaa gtctaatagc agatacaatg atgacaaggt gttggtttta
    13681 gctaaagagg gttggcaaca tggaataata ggaattgtag catcaaagtt aactgaaaaa
    13741 tactacaaac caacaattct tctaggtata gaaaatgggg aggctactgg ttcagcgagg
    13801 tcaataaaag gatttaatat atttgaagct ctcataaagt gcaaagactt gatgactaag
    13861 tttggtggtc atgaacaagc agcagggtta tccttggatt cagacaatgt agaaatttta
    13921 gcaaatgaaa taaataaatt tgcagattat aatttaactg aagatgatat gatagagaat
    13981 gtaaatgtcg agtttgaatt acaagaaaat gtaataaatt taaatcttgt agaagaatta
    14041 cataagttag aaccctttgg attaaataat cctaatccta gatttatagt tagaaattat
    14101 attttaaaag atttgaaagt tataggtaag aatcaacaac atctgaaatt aagtatagaa
    14161 aaagagaaaa gctatgaatg tataggattt aatatgagtc atttaaaatc aatgtataaa
    14221 gtaggagaca aagtagatgt actattccaa ttagatgaaa ataactatat gggaaatagg
    14281 aaagtacaat ttttacttaa agatatacga ttagctcgac ctaaaagtgc aagtaatgac
    14341 aaactttcat tgaaattaat gtcaaaaata ataccaaaag atactcaaag cctgtataat
    14401 atatctgtgt ctgattttga attatttgat ggaaatacag atataaatat atttgattat
    14461 tttgaaaaag atacactaat aatatctaat tctataaatg gattttatag agcaatgtca
    14521 gatatatcac ttatagattt ggattttaac ataaattaca atattattga agatgttagc
    14581 aaaaatactg ataaattaga gcttatattt tctcctaata ttgataaaat agacttaaaa
    14641 agatataata atattattct ttatgattat ttatacaata aaggcgaata ttcgtatata
    14701 tatgagaata aaagagaaga aagtgaaatt ataaaatatt ataataaaac agacctatta
    14761 tatttaaaga atgtagttag taatatagtt ccatcaagag atgaatttat aacaatttat
    14821 aaacaagcac tcataaaaaa agaaattgat ttggatatgg taaatataag ggaaactttt
    14881 aatgtaattc cattaaagtt ttttacgata ttaaatgtat ttagagaatt aaatttactt
    14941 gactttaatt taaattatga aaaaaattct gtactcataa gaatattgcc aaaaccacag
    15001 aaaaaattag acttaaatga gagtttaata ctcaataatc taaaaaattt agaaaaacaa
    15061 tacaatagca gttattagga ggcgtttccc atggatttaa aaaactttat aaggaatatt
    15121 gatgatttcc caaagccagg catagatttt aaagatgtga caactctatt taaagatgga
    15181 gatgcattta aatatgcagt ggattctata gttgaagaat tgaaagataa agatgtagat
    15241 ttagtaatag gaccagaagc aagaggattt ttgatgggta cacctgtagc atatgcttta
    15301 ggagtaggat ttgttccaat tagaaaacct ggaaagttac caggtgaagt tgagagttat
    15361 gagtatggat tagagtatgg tacagatact ttagagatac ataaggatgc aattaaaaag
    15421 ggtcaaaaag tagcaatagt agatgattta ttggctactg gtggaactat ggaagccgct
    15481 gccaagcttg tagaaaaatt aggtggagaa gtagtttcta tgcaattttt gattgagcta
    15541 aaattcttaa atgggagaga aaagttgtct aactatgatg ttaattctct tataaaatac
    15601 tagtactttt ataatagttg gtataacacc aactattttc atatatatta aaattttata
    15661 caaaataggt ggttatatgc atgataaaga attacaagaa ataattgaaa aaataaaagt
    15721 ttatgcacct aatgcaaatt ttgaaatggt tgaaaaagcc tataatcttg caaaaaaagc
    15781 gcatgaagga caatatagaa aatctgggga accatacttt attcatccat tagcagttgc
    15841 aaatatttta atagagatgg aattagatat tgaaactata acagcaggtt tacttcatga
    15901 tgttgtagaa gatacagaat atacctatga agacataaaa aaagaatttg gcaaagaagt
    15961 agcggatttg gttgatggag taactaaact tggtcaaata aaatatcgtt cgaaagaaga
    16021 aactcaatct gaaaatttaa gaaaaatgtt tttggcaatg gcgaaggata taagggttat
    16081 acttataaaa cttgctgata gattgcacaa catgagaaca ttaaaataca tgcctccaga
    16141 aaaagcaaag tataaagcaa aagagacact tgaaatttat ggtggtatag ctcatagatt
    16201 aggaatttcc aagataaagt gggaactaga agacagagca ttaagattta tggacccaga
    16261 agggtattat gatttagtta gtagagtatc aatgaagaga agtcaaagag aggattatat
    16321 tcaaggaatt gttgaacttc ttaaagataa gtttaaagat ttaagtatac catgtgatgt
    16381 atatggtaga ccaaagcatt tttacagtat atatagaaag atgcaaaaaa aacataagac
    16441 ttttgaagag atttttgatt taacagcagt aaggatattg gttgataatg taaaggattg
    16501 ttatgcagta cttggtatag ttcatacatt atggagacct attccaggaa gatttaaaga
    16561 ttatatagca atgccaaaac ccaatatgta tcaatcatta cacaccactg ttgtaggtcc
    16621 tgatggagaa cctttagaga tacaaataag aactcatgag atgcataata tagctgaaaa
    16681 tggtatagca gcccattgga aatataaaga aggtatctct agcaaagaat caaaggttga
    16741 agaaaaactt caatggttga gacagatgat ggagtgggaa aaagatttaa aagacccaca
    16801 agagtttatg gatgcactta aagaagatgt atttaatagt caagtttatg tatttacgcc
    16861 aaaaggagac gtaattgaat taccagcaga atcaacacct atagattttg cctatagggt
    16921 tcatacaaat gtaggtaata aatgtgtagg agctaaaatt gatggaagaa tagttcctat
    16981 tgattataag cttcaaaatg gtaatattgt cgaggtactt acatcatcta attctaatgg
    17041 accaagtagg gattggctta atatagtgaa gaccccaaat gcaaaaagta gaattagaca
    17101 atttttcaag aaggaaagaa gagaagaaaa tgttgaccgt ggaaatacaa tattagaaag
    17161 agaatttaag aagcatgata taccacaaaa agaccctctt gttgaaaaat atatggcatt
    17221 agttgctaag aaatttaatc agccaagtgt tgaggactta attgccactg taggttatgg
    17281 tggtataatg tcatctcaag tggtatctaa ggtaaaagac ctatatttaa aagaggttaa
    17341 aaaagctaaa aaggaacaac agatacaaga agaagattta aataaacaca atataagtga
    17401 aacagaatac agtaaaaaga gaaagaaaaa cgcatcacaa ggaattatag taaaagggct
    17461 tgacaatatc ttagtaagat ttgctaagtg ttgtaatcct cttccaggag atgaaattgt
    17521 aggatatatt actaagggta ggggagtagc catacacaga gctgattgcc caaatgcaaa
    17581 caataatggt gatttcttcc aaaatagatt ggtggaggtt tcatggagtt cagcaggaaa
    17641 aggtaagttt gaagcggaaa ttcaaataaa agctattgac agaagaggaa ttgttaatga
    17701 aataactcat attgttgcaa tggataaact tagtttaaac ggaataaatg caagaaaagg
    17761 taaggatagt attgtaagta taaacttact tgtagaggtt aatgacacag aggaattaaa
    17821 aattctaatg aaaaaattaa aagcaattcc tggtgtagaa agtatttata gaatgacaaa
    17881 ttagagttaa aataataggg aggaaactat gagagcagta gtgcaaagag tatcttcatc
    17941 taaagttaca gtagatgaaa acactatagg tcaaataaat aaggggttac tagtattgtt
    18001 aggagtgact catgatgata aatcaagtga tgtagattat atgattgata aaatattgaa
    18061 tctgagaatt tttgaagatg aaaatgacaa gatgaattta tctctaatgg atataggtgg
    18121 agagctttta gtagtatctc aatttacttt atatggagat tgcagaaaag gaagaagacc
    18181 tggatttagt aatgcagcta gaccagaact tgcaaataac ctttatgaag aatttgtaaa
    18241 aaaagcaaaa gataaaggtg tgacagttgg tacaggtcaa tttgcagctc atatgatggt
    18301 agagttaact aatgatggtc cagtaacaat actattagac tcaagtaaat ctttttaagg
    18361 gggaaaactt taatgattat aaaaaaattt gtagatagtt attttggagt aaatacttat
    18421 gtattaggtg atgaaaagac taaaaaatgt gcagttatag acccaggtgg aagtttagta
    18481 gagacattaa gttttgtaaa agaaaatgaa ttaaatattg aatatattat ccttactcat
    18541 gggcatggag accatatagg atatgttaag gatattaaag aaaaaactgg tgctaaagta
    18601 gttgcacatg ttgatgaaaa agaattatta aatgacaaaa ataaaaatct aagttataca
    18661 atgagatgtg gagcacaaga gtttgatgca gatatctatg taaatgataa agacaagctt
    18721 gaacttggtg aattaaaaat gactttcttg catactccag gacatactca aggatgtatg
    18781 tgtataagag ttgaagatga aatgttcaca ggagatactt tgtttgctgg aagtattggt
    18841 agaactgatt tatatagtgg agactttaac caaatggaaa aatcacttaa gaaattatgt
    18901 aaatatgaag atgctgttag agtatatcct ggacatggac caaccagtac tctaggggtt
    18961 gaaaagaaga caaatcctta catgtaattt tatattagta tttaaaaaag tataaaatat
    19021 ttaataacta tattagtgag ggggttcttc tactgttaaa tagaaggagt taagttgatg
    19081 ttgtatgttt ttttaaaagg acatgattat aagtatgaag tagcagaact tataaaattg
    19141 tttactagtg aatttaagtt taaagactct aatgctaaca ataatataga gtcaaagcat
    19201 ttaataaata gattgattta tgagaatggt gtattattct caagtactga atactatgaa
    19261 aatggaaact taaagtacga gtcaatacag aatatagaaa atatgaatct tgaattttta
    19321 gatggatttg atagcagcaa aacgattgaa gaatttctta atagcctaga tagtgaaagt
    19381 aagaggaact ttaaaaagct gtgtaaagaa aatatcaaaa aaagcatgtt tgtagtgctt
    19441 aaacaagtat ttaattctta tgtaccatgg ggaattttga ctggtattag accagtcaaa
    19501 attgtccaca atttaatgga taaaaagcta gatgataata gcataagaac cattctaaaa
    19561 gataattatt ttataataga tgagaaaata gatttagctc ttgaaatagc taaaagagag
    19621 cgattattta tgtatcctat agataaaaat aaaatttctc tttatgtaag tattccattt
    19681 tgtccaacta gatgtgtata ttgttcattt ccttcaaatt cattaaaaca atttggacat
    19741 ttaaaaagag aatatgttta taaattgatt gaagaaatta aggggtttgc caaagttatt
    19801 aaggagacta agaaagaagt tgaaactcta tatattggtg gtggaactcc aactacactt
    19861 gatgaggaag aattagattt gcttataaat tcattgttca atgaattaga tttaagtaaa
    19921 attaaagaat ttactgtaga agcaggtaga cctgacacta ttacagaaca aaaactaaga
    19981 gtgttaaaaa aacacaatgt tagtagaatt agtataaatc ctcaaactat gaatgatgat
    20041 accttggtaa agattggaag agagcataga gtatctgatt tagtagattg ctttaatatg
    20101 gctcgtagaa tgggatttga taatataaat atggatataa ttttaggtct tatagatgaa
    20161 gatttaaata tggtaaagaa tacattggag gagataaaaa aactttctcc tgaaagcctt
    20221 acagttcata cattagccat aaagagagca tccaacttaa atataaatat ggataggtat
    20281 aaagaacatc taacacaata tgaagaaatg gtaaaaatga tagatttatc aatgaaatat
    20341 gcaaaagata tgggattaaa tccatattat atgtatagac aaaaacatat gttgggaaat
    20401 ttagaaaata taggttatgc taaagaagga tatgagtgca tttataatat acagattatg
    20461 gaagaaaaac aaagtaacta tgcattaggt gcaggtgcta tatcgaaatt tgtttatgta
    20521 gatgaagata gaattgaaag agtagaaaat gtaaaaaatg ttgaacaata cattgaaaga
    20581 gtagatgaaa tgataaaaag gaagaaagaa gaggtatata aaaatgttga ctaaagctcc
    20641 tagaggaact aaagacataa ctccaaaaga ggcttacaaa tggcgttatg tagaaaataa
    20701 atttagagaa atatgtgctt tatatggata tgaagaaatg gtaacaccta tatttgaaca
    20761 tacagaactt ttcaagagaa gtgttggaga tactacagat atagttcaaa aagaaatgta
    20821 ttcttttaaa gataaaggtg atagagaaat taccctaaag ccagaaggta cagcaggtgt
    20881 agtaagagca tttatagaaa ataaattata tgcagatact caaccaacaa aattatttta
    20941 tgtaacacct tgttttagat atgaaagacc acaagctggt agacaaagac aatttcacca
    21001 atttggaata gaagcacttg gaagtgatac tccttcaatg gatgctgaaa taatcgcact
    21061 agctgttcaa ttttttaatg aagttggatt aaatgatttg gtagtgagta taaattcagt
    21121 tggctgtcca gtttgtagaa aagagtacaa tgctttatta aaagagtatc ttgactcaaa
    21181 agctgatata ttgtgtgata cttgcaatga gagaagagaa aaaaatccaa tgagggttat
    21241 agactgtaaa aatcctacat gtaaagaaaa tatcaaagat ataccattta ttgcagacca
    21301 tttgtgtgat gattgtaaat ctcactttga taaacttcaa gaatacttaa aggaaatgaa
    21361 tataaacttt gtaattgata aaacaatagt tagaggctta gattactata gaaaaactgc
    21421 ttttgaaatt atatctaatg atataggagc tcaaagtact gtatgtggtg gaggaagata
    21481 tgatggtctt gttgaacaat taggaggacc aaaaggtata agtggaattg ggtttggact
    21541 aggaattgaa agacttttac ttacacttga aggtaatgga attgaaatag aaaaccctca
    21601 atctacggat atatttatag taactatagg tgaaaaagca aatacaagaa gctttaaatt
    21661 gttaaaagac ttgagacaaa atcatataag tgcagataaa gaccatatag agagaagtgt
    21721 taaagcacaa tttaaatatt cagataaaat aaattcaaaa tttacaatag taattggaga
    21781 tgatgagctt aaaaatgaca ctgcgacatt aaaaaatatg aaaacatcag agcaaactac
    21841 tgtaaaatta agtacattag ttgaagaatt aaagcaaaag ctgtaaaata agaaagtatg
    21901 atgttttgtg tattagataa atatatagca ttaaaattaa ataaaatgga ggtagtatct
    21961 tggaaacttt aaagggttta aagagaactc actactgtgg agagttgaga gaaaaaaaca
    22021 taaatgaaga agtcgtactt atgggatggg tacagaaaaa aagaaattta ggtggtcttg
    22081 tatttgttga tttaagagat acaagtggat tatgtcaaat agtttttgat acagatgtag
    22141 ataaagaagc ttttgaaaaa gctgaaaaat taggggctga atttgtaata gctgtaaaag
    22201 gaaaagtatg cgaaagacaa tctaaaaacc ctaatatgcc aactggtgat atagaaatat
    22261 ttgcaactga acttagactt ttaaacaagt cagaaactcc gcctatttat ataaaagatg
    22321 atgatgatgt ttctgaagca ctaagattaa aatatagata cttagattta agaaaaccat
    22381 ctatgcaaag aaatttaaaa ttaagacata aagtaatgaa tattacaaga aattacttat
    22441 caaacaatag attttgcgaa atagaaacac catttttaat agctccaaca ccagaaggag
    22501 caagagatta tctagtacca agtagagtta atccaggaaa attttacgca ttaccacaat
    22561 caccacaatt atataaacaa ttattgatgg taagtggtat ggatagatat ttccaaatag
    22621 ttaaatgctt tagagatgaa gatttaagag cagataggca accagagttt acacaaatag
    22681 actgtgaaat gtcttttgtt gagcaagaag atgttatgag tatgattgaa ggtttgcttg
    22741 aagcgatatt taaagaagtt ttagatgtag aattggcact tccacttcct aagatgactt
    22801 atgcagaagc tatgtctaaa tatggttcag ataaaccaga tactagattt ggatatgagt
    22861 taacagatat atctgatgtt gtttgtaact gtggatttaa agtgtttgct gatgctacac
    22921 aaccaggaaa aagtgttaga ggtataaacg taaaaggcaa agctgacgac tttactagaa
    22981 aacaaatatc atcactagaa gaacatgcta agacatatag agcaaaaggt cttgcttgga
    23041 tgaaagtagg acaagaagga gtaacttctc caatagcaaa attctttaat gaagaagaaa
    23101 tgaacgctat acttacaaga atgaatgctg aagtaggaga tttactttta tttgtagctg
    23161 ataaaaactc aatagtattt gatgctttag gacaagttag acttgaagta gcaaataggc
    23221 ttaatctatt ggataaaaat gtatataact tactatgggt aactgagttc ccagtttttg
    23281 aagaagatga agaaacaggc acattctcag caatgcatca cccatttaca tcacctatgg
    23341 atgaagattt agacaagtta gaagaaggtg ataagtcatc tttaagagcg aaagcttatg
    23401 atattgtatt gaatggttat gaaataggtg gaggaagtgt tagaatatca aattctgatg
    23461 ttcaatcaag aatgtttaaa gcacttggtt ttacagagga aagagctaat gaaaagtttg
    23521 gttatttatt agaagcattt aaatatggta ctcctcctca tgctggtcta gcttttggtc
    23581 ttgatagact tgttatgtta cttgcaggaa ctgataatat aagagaagtt attgcattcc
    23641 ctaaaaatca aaatgctgtt tgccctatga caaatgctcc tacattggca gaagatgagc
    23701 aattagaaga attaagtata aaagttgata taaaagataa tgaataatat gtagttgcaa
    23761 gaaactaaaa tgtaaaaagt ctggatataa agtatattta tattcagact ttttttatta
    23821 gaattatttt atattttttc tctatatatt ttcataaatt atctttgtat tctcagccaa
    23881 tataattaaa atactatgtt taaaaatttt acataaactt
//
LOCUS       DAELNY010000045        22723 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.045, whole genome shotgun sequence.
ACCESSION   DAELNY010000045 DAELNY010000000
VERSION     DAELNY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.045"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            85..921
                     /locus_tag="KPE55_001531"
     CDS             85..921
                     /locus_tag="KPE55_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0492620.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1274..2851
                     /locus_tag="KPE55_001532"
     CDS             1274..2851
                     /locus_tag="KPE55_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0492621.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2844..4028
                     /locus_tag="KPE55_001533"
     CDS             2844..4028
                     /locus_tag="KPE55_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0492622.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4046..4645
                     /locus_tag="KPE55_001534"
     CDS             4046..4645
                     /locus_tag="KPE55_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0492623.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4669..5139
                     /locus_tag="KPE55_001535"
     CDS             4669..5139
                     /locus_tag="KPE55_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0492624.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5498..6244
                     /locus_tag="KPE55_001536"
     CDS             5498..6244
                     /locus_tag="KPE55_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG0492625.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6305..6979
                     /locus_tag="KPE55_001537"
     CDS             6305..6979
                     /locus_tag="KPE55_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0492626.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7348..8694
                     /locus_tag="KPE55_001538"
     CDS             7348..8694
                     /locus_tag="KPE55_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0492627.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGRGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8721..10121
                     /locus_tag="KPE55_001539"
     CDS             8721..10121
                     /locus_tag="KPE55_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492628.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10331..11767
                     /locus_tag="KPE55_001540"
     CDS             10331..11767
                     /locus_tag="KPE55_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0492629.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIQ"
     gene            12111..13055
                     /locus_tag="KPE55_001541"
     CDS             12111..13055
                     /locus_tag="KPE55_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG0492630.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13224..14156
                     /locus_tag="KPE55_001542"
     CDS             13224..14156
                     /locus_tag="KPE55_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG0492631.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKVK"
     gene            14261..14728
                     /locus_tag="KPE55_001543"
     CDS             14261..14728
                     /locus_tag="KPE55_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG0492632.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(14972..15826)
                     /locus_tag="KPE55_001544"
     CDS             complement(14972..15826)
                     /locus_tag="KPE55_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBG0492633.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYMENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            15992..17956
                     /locus_tag="KPE55_001545"
     CDS             15992..17956
                     /locus_tag="KPE55_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0492634.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            17956..18417
                     /locus_tag="KPE55_001546"
     CDS             17956..18417
                     /locus_tag="KPE55_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0492635.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18420..18740
                     /locus_tag="KPE55_001547"
     CDS             18420..18740
                     /locus_tag="KPE55_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0492636.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18756..19856
                     /locus_tag="KPE55_001548"
     CDS             18756..19856
                     /locus_tag="KPE55_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0492637.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            19976..22576
                     /locus_tag="KPE55_001549"
     CDS             19976..22576
                     /locus_tag="KPE55_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG0492638.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLKGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         8915 a         2348 c         4022 g         7438 t
ORIGIN      
        1 agtaaaattt taaatattga cattaaatta aatatactat actatgtaag tgtgatgaga
       61 gaattttttt agaggggatt tatagtgaat ataaaaaaga tatttaataa caatgtagta
      121 gtaagctccc tagaagatgg cacagaaata attgtcactg gtgctggagt aggatttaag
      181 aaaaaagtag gcgatttgat agatgaaaat ttgatttcta agaaatattt tgttcaagac
      241 gatcaaaggg acaaatacaa tcaaatactt aataagacct ctattgaata ctttaaaatt
      301 tcagaagaaa ttatagaaaa ggctaatgaa gttttaaata ctcaagtgaa tgattcaata
      361 atattagcct taactagtca catagaattt gctgtccaaa gagaaaaaca aggtataaaa
      421 ttaccaaact taatattgaa tgagacaaaa caactttata gggaagaatt tgaatttgga
      481 ttatgggcaa ttgatgaaat tgaaaaaagg ataggaataa aattgcctga agatgaagca
      541 ggatatattg ctattcacat aatcaatggt ttggaaaatt ctcaaaaaga tgaaggtatt
      601 aatatactag agttttcaaa acaagttatt cagataattg aagaagtaca tggatttaaa
      661 ttggatgtaa attcattaaa ttatactaga cttataactc acttaaagtt ttttgttcaa
      721 aggattctcc gaaaagaaac ttacaatgat agtgatattg aagatatgta taagttagtg
      781 aataaaaatt acaataaacc taaagtgtgt actgaagaaa ttgcagcatt ggtattagac
      841 agatttgaat ataagattag taaagaagaa gaattatatt taatgattca cataaataaa
      901 ataacaaaca gtggttgtta ggattgtaac tgataaagca ggcaaaacct aagtcattga
      961 gcttaactat tagattaagt tttttgattt aggttttttt attagataca atcaaaatac
     1021 agacagtgac ttttaggatt gtaactggtt aagcaggcaa aacctaaatc attgaattta
     1081 actattaggt taagtttttt gatttaggtt tttttattag atacaatcaa atacagacag
     1141 tggttgttag gattgtaact ggttgagcag gcaaaaccta aatcattaaa cttaattatt
     1201 aaattaagtt ttttgattta ggtttttcta taaatttgat tatgttttta gataaaaata
     1261 ggaggggaaa attatgaaaa aaaataagtt aggtttatgg aacttcttcc aaatgttagg
     1321 aaaaacgttt atgttcccaa tagcactact tagtgtttct gggatgatgc taggacttgg
     1381 ggcaggtttt acagacccta agatgattga aatggttcca tttttaggga atgaatatgt
     1441 taacttagtt ttaaacttca tgttaacaat aggattattt gcatttaaca acttgggagc
     1501 attatttgca atggcaattc cactaggatt attaaaaaaa gaaaaagagt ttggtgcttt
     1561 ttcaggatta gtaggattta tagcaatgca tataggtaca aatttttatt taacaagagc
     1621 tgatttgtta gtaccagcag aacagatgtc aactaatggt caagctatga ttatgggtat
     1681 acaaacatat aatacaagtg ttttaggtgg tattgtagca ggattaattg tttatgcaat
     1741 atatgataaa gttagtaatt taaagatacc agaatcatta ggattctaca gtggaccaag
     1801 attagttcca attgttagtt taatagttat gagtatagtt ggattattaa tacctattgt
     1861 ttggccacca tttttcaatg cattccaaag tttaggaaga tggatttctt cagcaggacc
     1921 attaggatat ttttcatatg cagtggcaga aagagttaca attccatttg gactaaatca
     1981 tcttgtaaca tctgtattcc gttttacacc aataggtgga actgctgtta ttgatggaga
     2041 aacgtacttt ggtacattga acatgtttat ggcatatgta gaaaataatc agattatacc
     2101 tcttgattta gctggtaaaa tggaacaagg taaattgatg attcaatatg gattagctgg
     2161 tgctgcattg gctatctacc gtacagcaaa accagaaaat agaaaggcac ttaaaggact
     2221 tttaatatca ggagtgttga cagttattat aggtggtata agtgaaccaa tagagtttct
     2281 attcttattc atatcaccag cattatttgc tttccataca tttatgaatg gtctagcaaa
     2341 catggtactt ccatacttag gcgtgttgat gggattcaca ggagatttaa ttgcatttat
     2401 aagttttggt gtgttaagag gaacagctac aggatggcca atagcagtat tagtagcagt
     2461 tttatatttt gcaatatact attttgtatt taagtgggca attattaagt ttaatataaa
     2521 gaccccagga cgtgaggata aagaaataaa agtatctgaa ggaaatggtt caaatttcaa
     2581 aaatttgtct acatataaag gacaacaaat gatatctgct ttaggtggtc aatctaatat
     2641 attatcacta gataattgtg taactagatt acgtttaaaa ttacaagatg tatcattaat
     2701 aaatgaggat gcaattaaag aggctggtgg tatagcagtt gttaaattgg atgagcatac
     2761 aatacaagta attataggaa cccaagtgta ttctttaaga aagcagatgg ataaagctat
     2821 ggaaggtcat ttggaggcat gtaatgatta attttgataa aaaagtagac cgattgggta
     2881 catattgtac ccagtgggac tatgtagaag atagatttgg taagaaggat ttgcttcctt
     2941 ttacaatttc agatatggat ttagagtttc catttgaaat aacagaggtg ttgcaggaaa
     3001 ggttaaatca tagagttttg ggatacagta gatggaaaca tgatgatttt agaaattcag
     3061 ttacgaattg gtatattaaa agatttaatt gtagcgttga tagtaactgg atatattata
     3121 gtccaagtgt aatgtattca attagcaaac ttattgagat gttaagtaat gaaggagatg
     3181 gaatccttat aaatactcct gcatacaatg ctttttatga tgtgataata aataacaatc
     3241 gtaatattat aaagtcacat cttgtaaata ggaatggatt ctattcaatt gattttaatg
     3301 attttgagaa aaagtgtaaa aaatctaaga tatttatttt atgtaatcct cacaatccaa
     3361 ctggtagagt atggagtgag ttagaacttg ttaagatgat aggaatttgt aagaataatg
     3421 atgtcaagat aatatcagat gatatacaca tggatattgt atatgacaat agaatgacac
     3481 ctatcttaaa tgttgcaaag gactatttag attctgtatt catttgtaca tcagcgtcta
     3541 aatctttcaa tataccagca ttgactgggt catatgtgat tataccaaat gaaggtatta
     3601 gaaataaatt tgaaaatatc actagatacc gtgattttgt aaattcgcca gcaattctag
     3661 caatattagc cacaatggtc tgttatgatg actgcgaata ttggttagat gaactattaa
     3721 aataccttaa agagaatctt aaatatacga ttgattatat aaaagataat ttagaaccat
     3781 tgaaattggt catgccagaa ggctgttact ttgcttggat agatttttcc gaacttaaaa
     3841 taagtggtga gaaattccaa aaattattga tggatattgg agaagttgct attatgtcgg
     3901 ggtctgtata tggtgaagaa ggtgagtttt atttaaggtt aaatgtggca tgttcaagag
     3961 aaaaacttga agatggttta aataggatta aaaaaactat ggattatatt gaaaaaagaa
     4021 ttttatagta ggaagggaga tagttatggg atatttaaaa acaatggttg atgttatgac
     4081 tactgaaatg aatgccataa gatgtttaat agatgaagca ggaatagaat atgaaagtat
     4141 agttagtgaa attgcaaatt gtgaaggcaa agtaattttt atgggtgtag gaaaatctgc
     4201 acatataggt aagaaattgg cagctacatt tgctagtact ggtacaccta gcttttttgt
     4261 acatgcaaca gaggcagtgc atggagattt aggaatgatt gaaagtaagg atattacaat
     4321 tttaatatca aatagtggaa attctatgga agtggtaaac tgtataaaat atattaaagc
     4381 aataggttct aagacaattg catttacttc aaacagaaat tcagtactgg caaaagagtg
     4441 tgattatgct ttaatatatc ctgcaaagga tgaagcagac catttaaatc tagcaccaac
     4501 aacatcatct acaattactt tggttttagg agattctatt gcatgtgcat tatctaagag
     4561 tagtaatttt ggttcttcag atttttataa gtaccatcca ggaggaagtt taggtgagaa
     4621 gttaaaaaca gctaacaatg gataaaagca aaaaggagag tagtttttat gttcaaaatg
     4681 tttaaaaaaa aatcaataaa atcacccata tcaggaaaag taatagagtt atctaaaata
     4741 caagatggtg tattttctca aaaaattatg ggagagggtg tagctataga ttctacagga
     4801 gacattgtat atgctccaat caatggaaag atagtagtag tagcagagac taaacatgca
     4861 tttgtaatag aattagaaaa tgggatggaa cttcttattc atgttggttt agatactgta
     4921 aatttaaaag gcgaaggttt tgaggtattg gtgagtatgg gagaacaagt gaaggaagga
     4981 acaccaatgt tgaaaattaa ccgtcctttg attgaatcaa agggtatatc acttattact
     5041 ccagttacaa taactaatca ttcagagtac aatatgaata catgtaatgt aggcaatagt
     5101 gttgaaggtg ggaaagatac tgtaattgag tttaaataaa gaactaaaat acaaggttaa
     5161 tatttataaa tacctataaa cttatgtttt ataagattga ttgtatagat ttgttgtata
     5221 aaaaagaact ctttaattat aaattaagga gttctttttt attttgtaaa tattgtttag
     5281 atttatatag aaaaacactt agtaataaat agaataaata agtatttata tagctaaaaa
     5341 tctaaatagt tttgtttaaa tatattttta tattttgtaa tttcaaatta ataaataatt
     5401 agatttgata aacaaaaaat aaaaatgttg attacattaa taaactttaa tataatatat
     5461 ttatgaaaaa tgtaaactgt gaaatggagg tagtaacatg aaaattgaaa atctttctat
     5521 tgatgatatt aaacgaggct atatatttaa tgatgattta aattgttata gttgcattaa
     5581 atgtacaaaa acatatgagg taggagaaat atataaaata ggtaatagat tctttgaagc
     5641 ttcaaaagct attgaacttc atatggaatt agaacatagt gattatttag agcaattgct
     5701 taatagtgat tcaaagtaca acacattgac tgaaaatcaa aaacaattat ttcaattatt
     5761 tattagtgat ttaccagata aggagatagc aaaacaggtt ggagtatcca catctactat
     5821 acgccatcaa aaatttattt tcagagaaaa agcaaaacag gctaagttct atttagcact
     5881 atatgaatct gtatttgaag ataaatctaa taaaagtagt actataatac caatccatga
     5941 acatgctcga atggtagatg aaagatatgt agtaactgaa gaagagagaa aacacatttt
     6001 ggatacatca tttgaaagta tgaatccttt aaaattaaaa gtattttctt caaaagaaaa
     6061 gaaaaaggtc gtaattttat ctaagatttt ggaacaattt gaacgtggga agaaatattc
     6121 agaaaaggaa gttaatcaga ttttagaatc tatatatgat gattttgcaa caattagaag
     6181 atatctaatt gagtatggtt ttatgagtag aaataaagaa tgtacaaaat attggttaac
     6241 ataattaaaa taataaattt tactaatgtg taatgttatt tttatctagg gggaggagtc
     6301 aaatatgaat gatgaattaa aatctatttt atacaataaa ggtgtagaca ttgttcgctt
     6361 tgttgatata tctgaatttc caataaatca aacacaaggt ttttctaaag caattttatt
     6421 ttgtataggg ttatcaaaaa aatttattac agatatatac aataacttac ctacagatag
     6481 tgatgagttt ttggaaaaag aggagaaagt agaagaatta gctgactgga tttcaaaata
     6541 tattcaaaat aaaggatata gagcgtattc tcaatcggag aaaaacaatc tagaacatgg
     6601 atattttgaa aaagcctata ttaatccaga aatgcaatca gggattagtc ctttaccaca
     6661 taagacaata gcaaatatta gtggcattgg atttatggga aaaaataatt tgtttgtaac
     6721 agaggaatat ggatgtgctt ttagcatgtg tacagtactt acagatgctc caatttctgt
     6781 tgaaagatat cctttgattg attcaaaatg tatggattgc aatgtttgtg tagagaactg
     6841 tcctgcaaaa gctatacatg gaaatgaatg gactttacct ggaaaaagag aatctattat
     6901 tgatgtttca aagtgttttt gtgtattgag gtgtatgatg agttgcccat ggtcattaag
     6961 atatgcaaat caaaaataac tttactagag aaggtattaa attgaatatt ataaaatttt
     7021 taaaatgtaa gattatataa gatttttcaa aataaaaatt tgtatagaac actcagaaat
     7081 gagtgttttt ctattgggat taaattatat tgatacaaag cttaactatt ttaatcatta
     7141 tataaaattt gtattaaaaa attaagaata acttggtaat agtgaaaata taatgagatt
     7201 ataattgatt tataatataa ctataatgat attgtaaaaa tgaaaattgt cctctcaaaa
     7261 ttcccaaaga gagtaaaagt cacttcagtt atagtcaaaa aaatttttct gtgaaaagaa
     7321 tttagatttg aaaaggagga attaatcatg gaaattgaaa ctaaaactcc agattggatt
     7381 aatgtactag aattaaacag taaaatagat attactggga aatatgatgt aagtaatatg
     7441 atacaaaata ttgtctctga taagtcacta gaaggttcca ttatatattt tccaaaaggt
     7501 aattacttat ttgagaaagg tattaaaatt agtcaacaaa taacacttca aggagattct
     7561 tattatggaa gaggtaatca agtatcaaat ttagataaaa aacaatcaat cataggaaca
     7621 accaatttta taacccgagg agtttctaat atgagtatta ttacattgtc aggaacaagt
     7681 caatgtatca aaaatattaa tttttattat gatagtcatg atatagagaa aataccgcct
     7741 aaaaatgtat ctgctattac agaatatgga gaaacacaag gactatctca ttttgaacat
     7801 ctgtttattt ctggattttc agggatagga attgaaattc catactattc aactggtaat
     7861 gatattactg ttagttcatg tggtcttggt atgaggcttg gagagaagtc aatgctttct
     7921 agtagcaaga tttacgaatg taaaaatgga atggagatta ctactggtgt tagcttaaat
     7981 aatgtgagaa tagaagaaat aaaagaaata ggtattaata ataaaggatt tggttttaac
     8041 ctgattatga atcttatggt tgatcaatgt ggatattgtg gatttatgtt tgaaaaaatg
     8101 tgctatagcc agataactgc cagaattact agatgcggtc agtattataa aagtgttgat
     8161 tataatgctt atgagaatat ggaagatagg aaagaagaag cttattccat tttttatgga
     8221 gatattttag aaaattgcaa tattgtattg atgaatagaa atattgatac cttagaaagt
     8281 gattcactta atgaacataa gatatatgta atcaaggcaa atgaaacaaa caatgtaatg
     8341 ctaacatgtt atgctgaagc agataacttt atacaatgtg caaagggcaa cttgctgtta
     8401 gaaaatggtg aaaatactta taaattttat aatggtgaga tttgttctgt aggaggtgtt
     8461 caaatttctg atatagatga aaaagattta atcaaaataa tagagaacac tttgtatata
     8521 gatgataaag gtaagtattt aatggttcct agattaaaaa agaatactgt aatatattca
     8581 gtactagatt ctacagaaga aataaataaa ttacatggtg gaatatggga acagattgat
     8641 gtaaaagtat tagctgggga atccatgtac tattataaaa agataaaaga ttaaattatc
     8701 aaactaggag gtagtgatta atggaagagt ctataataat taaagatata agtggctgga
     8761 ttaatgtact tgattatgga atttgtaatg atgaaacaaa agatgtaagt tttaagttgc
     8821 aagagattat ggatgctgta ccagaagatt ctattatata ttttccaaaa ggaaaatact
     8881 tatttgaaag tggtgttaag attaagaaac gattaacctt atgtggagat tcttatattt
     8941 tagcgaatgt tcctaaaaat actgctggag taacacagtt tacttttaaa ggaaagaaaa
     9001 atcctaaaag tgaaaataga gcaatcataa cagcaaaaag tgtaagacac tgtattaaga
     9061 acatttgctt ttattcagat agttgtgata gagatatgac atctaatcat gaacctccaa
     9121 cagatggaaa acctagattt catcataatg taattataaa ttatagaaat atatctgctg
     9181 ttatatgtga agataaacag ggagaaccag gtcattatga aaatctatct tttagtggtt
     9241 tttctggaat tgcacttgat atgcctaata attctacagc aaatgatatt acaattttta
     9301 catccggact tggaattagt acaggggaaa attcaacaat tgctaacagt aaggtttggg
     9361 gctgtgttga tggaatgata atttctactg gaacttttat aaatggtatg agggtagaag
     9421 aaataaaaaa agttggaatt aaaaatattg gtagaggttt aaattttatt acaaatatca
     9481 caattgacca atgtggatat tgtggagttt ggtttgattc tatatccaat gttcagatat
     9541 caggaaatat cacaaggtgt gggcagtact attatgatgt aagctatgat aattatttaa
     9601 tgttggataa tagagttaaa gaagcatatt ctttattcta tggtaattta ttggaatcaa
     9661 gtaatatagt tttatcaaat accaataaag ataattggga agatttcaaa gaagataagc
     9721 ataaggtgta tcttgtagaa gcacttgaaa ccaagaatgt actattaaca tgcaatatag
     9781 atgctactga ctggattgtt aagtcagaaa aaggaaattt aacgtacaat aatatgcgac
     9841 aaactgttat attttacgat ggaaaactaa gtagtatcaa tggaattggt atatctgatg
     9901 aagattatgg ggataaagtc aagataaaaa aaggaaatat atatataaat agtaaggata
     9961 aggatattaa gatgcataaa ccagaggtag gtgacattat gtcaacaatt attaaagata
    10021 aaagtattct ttctgctcat tatgggggaa catgggagaa aattggtgaa aaaatagagt
    10081 ttggaataac tgtacactat tataaattga tagagaaata agtaaatacg tagtatttga
    10141 atagatgtat tttattggag agctaggact atagttttaa tgtaaatggt ttggtaatag
    10201 catggattga ataatataaa aataatataa aaaattaatt tgattatgta aaattctatg
    10261 aagtagtaaa actgtgactt tagtcatagg aggttcaaac taagtaaaag ggaaaggaat
    10321 atataatatt atggaaggaa gaactgggat taaagatgga agatggatta atgtagtaga
    10381 aagaggtatt aaaaatgatg gttcgtacaa cataggtcct gctttgcagg atattattga
    10441 tgaaatagaa acagattttt gtgtcttata ttttccaaaa ggtaaatacc tatttacaag
    10501 tggtgttaaa attaataaag aaattgtatt gcaaggtgat tctaattcat ctacaggtac
    10561 tacacaattt ataactcgtg gagtagaaaa tatgagtatt attacattga cagggaagaa
    10621 acaatgtatt aaaaatataa atttctattc tgatagttgt gagatacagg taaataaaga
    10681 accaccgaca aaaggtaatc ctaaatatca ttatgagatg attatcaata agaatgaaaa
    10741 gaatgttgag atgaacaatg tatcagcaat cctgtatgat aacagtaaga atatgattaa
    10801 aggtctaggc cattatgaaa atatttatat aagtggtttt tcgggtacag gaattaggat
    10861 accatattac tcaattgtaa atgatattac agtttcatct tgtgggctgg gtattgatac
    10921 aggaatagat acaattattt ctaatagtag aatatctaag tgtcaaaatg gaatgaggat
    10981 taggactgga actagcataa ataatgtaag aattgaaaaa atacagaaag ttggtcttat
    11041 aagtagtcaa gttaaggatt atgagggata tggaagttat aaaattaata atattacgat
    11101 tgaccaatgt gggtattgtg gttttttatt tgactcagtg aaaggtagtt atttttctgg
    11161 tattataaga agatgtgggc agtactatta taatacagat tatgacacat atttaagtat
    11221 taaagataga gtagaagaag catattcttt attttatgga aattatttta cagaatgtaa
    11281 tattgattta gttagtgata acaaggataa ctgggatgat ggcttagaat ataaacataa
    11341 agtttatgta tttaaaaccc ttcaaatacc aaaattaaca ttgagatgca atatagatgc
    11401 ctatgattat atcgttaaat cggtacatgg aggaggtaat ttaatgttaa aaaataactt
    11461 taacaattat atatttattg attcatctaa tccagaagtt attaatggaa ttaattttaa
    11521 ttatcaaaag aatggaaact tgttaaggtt aaataatggt aaattaagtt ataaaaatgg
    11581 aaaggaagaa acagctatat tcagacctaa ttatggtctt attgtagcat caacagattc
    11641 agaatgtgaa caaatagaga ctagctatgg aggaaaatgg gagaagcttg gttctgaagt
    11701 aattggaaaa gaaacggtat attattacaa acatcatgaa ccagataaaa caaaccaaat
    11761 acaataaaat ttgtattata taatgatata aagttttaat atatagtgtt tatctttaat
    11821 gtaatattgg tatatttgta tagtattaaa tttgttaaaa agtaaaattg tagggtgctt
    11881 gaaaatagca ccttttttag tttcattata aaattagttt tgaaagttta ggaataatgt
    11941 atatttattt ttaaagaatt ataaccttat taaggatatt ttaaatgcta gaaatgaatc
    12001 agtaaatcat actggaatta tcttaaatgt tttgattaaa ttaagtattt agagctataa
    12061 ttatattaaa aatatattag aacataatta tattactatg gaggttaaat atgatagtaa
    12121 gtgtgagtag aagaactgat atacctgctt tctatagtaa gtggtttttt aacagactta
    12181 aagaaggttt tgtttatgta attaatccaa tgaatccaaa acaggttagt aagatagagt
    12241 taaatccaca tactgttgat tgttttgtat tttggacaaa ggatgtaact ccaatgttat
    12301 atgatttaaa taaattaaaa gactataaat attattttca ctatactata acatcatatg
    12361 gaaaagaagt agagacatgt attttagata aaagaaaggt aatagattca tttaaggaat
    12421 tgtctagaaa aataggcaaa gaaagagtaa ttttaagata cgacccgatt ttcttaagtg
    12481 aaaagtacac agtagattat catatcaaag catttgcgag tttatgtaat cagcttgatg
    12541 gatttacaga aaaatgtata ataagtttta tcgatttgta taagaaaaca aaatttaata
    12601 ctaaagcatt acatataaag ccaatagaaa ttacagaaat agaaacatta tcaaaagaga
    12661 tttctaagat tgctcataag catgggataa tacttgaaat gtgttctgaa gaatataatt
    12721 taagtaaatt tgggattgga aaaagtaagt gtattgatga tagattaata tcaaaaatta
    12781 taggatgtga agtagaggta aaaaaagata gtacacaaag agatatttgt ggatgtgtta
    12841 aaagtgtcga tataggacaa tacaatactt gcagacatta ttgtctgtat tgttatgcta
    12901 actttaatta tgcaaaagta gaagaaaact gtaggcttta taaggaagat aataaattac
    12961 tcatagatac tttaagagat gatgcaaaaa taagcattag agatatgaag actattaaag
    13021 tagataagaa taaacaaatt agtatatttg attaaaataa atatactaat taccttaata
    13081 tattaagaat atagatactt ttgataataa aattaaactt aatagatatt ttctatataa
    13141 gaaagatggt cataatatgt caatttgacc atctttttga tataattaaa acacaaataa
    13201 tttagtaaca aaggaggcag aacttgaatt tattaaaaaa atatcctata cctattgcaa
    13261 gtttgatttt gtcaatcttt actttaggta acttattaca atcttatagt gaatcattac
    13321 gtaatgtaat tggatttata ggatttattt tgtatgcaat ttatatagtc aaaataatat
    13381 tacttagaaa gggtgttaaa gagcaattag agaacccttt aatatcaagt acatttccaa
    13441 ctataacaat ggcaacaatg atatttgcta catatataaa accattatct ttagaattaa
    13501 gtattatgat ttggagcata ggaattgtgg gtcatcttgt gttaatagtc ttatttagta
    13561 agaagttctt agttaaattc tcaataaaaa cggtatttcc atcttggtat atagtatatg
    13621 ttgggatagt tgcagctagt gttacggcac cagcatttaa tcaacttttg attggcaaaa
    13681 ttgctttttg gataggattt gtatcttata tagttcttat accagtaatt ttaaagagag
    13741 tatggataat caaggaaatg cctgaaccat ctttaccaag tatagctata ttagctgcac
    13801 cagggtcact attattgtct ggatatatta attctttttc agataaaaat aatgtagtat
    13861 tatacttgtt attcatatta tcaattgtat tttatattat agttttggga tacttaccga
    13921 aattattaag attgcctttt tatccgagtt ttgctgcatt tacattccct atggcaataa
    13981 gtgcaatggg tatgaaacta atgaatggtt atttaactaa atcaggtcat tttataagtt
    14041 ggatttcata tgcttctaag ttagaagaga taattgctac agtgattatt ttatatgttt
    14101 tagttttgtt tattatatat cttagtaaat ctgaaactac atctaaagtt aaataagaat
    14161 tattaaatat gtgtatttaa taggaatatt gatataaata tatgaagata agataaacca
    14221 tagaaactta atttagctta attttattgg aggaaaacaa atgaaaacaa aagaagaata
    14281 tttaaaagaa gttagaaaca tatcagaaga atactttaga aaaggagagt ttttttgctc
    14341 tgaagcagtt ctccagacaa taaatgatgc tttaggccaa cctttaagtc cagagataac
    14401 taagttagca tcaggatttc cgataggact tggaaaggca caatgtcttt gcggagcaat
    14461 atctggggga gagatggctt taggtatagt atatggaaga gttcatggtg aagctatgaa
    14521 tccaaagatg tttgagcatg ctaaaggttt gcatgattat attaaaaaag agtatggtgc
    14581 aacatgttgt agagctatta caaagaagtg ggatggtgat aactttatgt ctcccgaaag
    14641 aaaagagcac tgtattaaaa taacaggtca agttacagag tgggttgcag ataagcttat
    14701 agaagatgaa aaacttaata tcaaatagtg caatattagt tggtgaattt ataaagttaa
    14761 atttacttat agtataagat acttgatatt ctcgtattga tagaaaaatt tatagcatat
    14821 gtatttaatg aaatatactt atatttagag tgtggtttaa tttcataaat acatatgcta
    14881 ttattttaag tatataagca aatttattta aataaataga aacagttaat ttatatgtac
    14941 aaaactatat atgattagtg taaataaata cctattttat aatactcaac ataaagacac
    15001 cagacatgat tagtattata ccaataaaaa tagcaagcca ttcttttttg ttcttctttt
    15061 ctttaaaaaa tataatccct cctaaagtgg ctactatcac acaactctga gagatagaaa
    15121 aacttattgt aactcccaat gtattagtcg ttatgaacat acctaaattt gctattgacc
    15181 acaatatacc agtaattaaa ttgaataaaa cattttccat atatacaatc tctttagaaa
    15241 aatacttgac aataatagat gaagttatca acataccaac agattgaggt aatataattc
    15301 tatatccttc aattaaaaat atttgattag ttacaacata taatgataaa aatagagatg
    15361 ataataatac aactccaaca cattttaaca tatttttatc tgtagatttt ttagtttgat
    15421 aatttgtgag aagtatacca agtattatac ataatattga taaagaacca atcagtaaca
    15481 ttttagaact ggtccattca ttaaacaaga ttacagctaa taatgtcgta aaagttaatt
    15541 gtgaaccatt tgaaattggc attactttag atacactagc aatttgaatt gctttaaact
    15601 gcattaattg tcccagactc cagaatatgc cagaaatcat acttatacaa aatgaaaaaa
    15661 atgtaatttc aggatgtata accagagtta ttatagtgga aaatataaac gcacttatgg
    15721 atgtacctaa aagctgttca tgaggagaac ttttctttag atttgcaact atgggcatca
    15781 atccccatcc cattacagga agtaaagcaa taattattgt cagcattaaa ctatttcctt
    15841 tcatattaaa tttagacagc ttttaattta tcatctaaaa ttaaagtgtt caatacataa
    15901 aaattaccgt atttaatcag gaaatattag tagtttttta gaaaaacatt ttccattaaa
    15961 ataaagacaa agggaaggag taaaattgaa aatgttaact gatatgattc aaaatcgtga
    16021 aattaaaata ataaagtttt tattaaaaga aggtcataca actgtaaaag caatagcaga
    16081 tgatttagat gtttcagaaa aaacagtttc aaaatctctt aaagaagtag aagcagtgct
    16141 taaaaagata ggtctttctt taataagaaa acctaagata ggtgtttata ttgatggaga
    16201 tttaaaaaaa atacttcctt acctagataa taaaggaagt caaattccta caaccagaga
    16261 ggagagagtt atctatatat attcagtact tttaaagagt aatgattata ttactataca
    16321 acaactttct gatgaactat atataagtcg tggaactatt gaaagggatt tagcagaagt
    16381 tgaaaaactg ctaaaaaacg aagggatttc catatataag aaacctagta agggaataaa
    16441 gttaaatata agtgaaagag ataaaagaca acttactgct aagtttatac ataaattttg
    16501 gagtagaaac tggtatatta agcaagagca agaaagcttt tatcaagcat ttgaaaagat
    16561 acaagcagat gtaaatggta ttttttcgga agatgattta tcaataatta ttgatattct
    16621 taggaatttc agcagagaaa gaaaatttga gttctctgat tatgaatttc agtcattagt
    16681 tattcatttg gcaatagctg tagagagaat tagaaaaggc gaatatatag aagaaagttt
    16741 gtatactggt aatatagaaa ataatcaaat gtcaaatacc aattatttag caaaacaatt
    16801 agaagaaaaa ttttcaatat tattaccaaa atctgaaatt ggatatataa atttacattt
    16861 agtagcgtct gaccaacata gagatactaa tgaactaaaa aatacttcaa ctaatccttg
    16921 tgagacaagt acaaataata tatcaaatct tcgggaaatt ataaaaaata cattgtatga
    16981 gacagcttat gacaaggatt taattgatgg attaacttca catgtacatt catcaataaa
    17041 tagactgaaa tatggactta gtattaaaaa tccatatgta aatacaatca aacaaaactt
    17101 ttctttatca tttgagcaag ctttagattt aaagaaagta attgaattta tatatgatat
    17161 taggataaat gatgatgaat gtgcttatat agcattacat tttgaagctt ttagagaaag
    17221 aatgaaagaa cttccacaaa atattagagt aattctagta tgcagtacag gattaggttc
    17281 atctaggtta ttgtctgcaa gaataaaaaa atactttcca atgataaaga ttgaaaaagt
    17341 actttccgta caagaattag tatccagtaa aattgatgca gatttagtaa taagtactat
    17401 ttatttagag ttagaaacaa ttccaaccat agtagttagt cctattttaa atgaaagtga
    17461 tataagaacc ttggaaagaa atatacaaaa tgtttcaagt tatacttcta agaagtataa
    17521 atctttcaga aacttaatct ttgaagaaaa tatattgttg aatattgaag cttcaaacta
    17581 tgaagatgca attaagtata taacagataa cctaataaaa aaaggttttg caaaagaagg
    17641 tatagctaaa agtgcaattg agagagaaaa tttatcttac acttctttcc aatcaatagc
    17701 tacacctcat gctaatccta gttatattaa aaaatctaat atatctgttg caacgttaaa
    17761 aaatcctatt acatggggaa atgaaaaggt ggaagttatt ttctttattt cattagaaaa
    17821 tgacaaaaac ctagagcttg atgaaatata tgaagttttt tatgacttca ttgacaataa
    17881 aaaaaatata aatgacatat taagtgcaac ttccacaaag gaagtgtacg atttgctagt
    17941 ggaggagagt atatagtgaa aatatcagat gtattgaaaa aagagcaaat tgtttttaat
    18001 cttgatacaa atacaaagga agaaacaata aaaaaattaa gtagtatatt tgttgaaaat
    18061 gggattgtgg acaatgaaga agaatatgtt aagtctgttt tggaaagaga agaacattca
    18121 actacaggta ttggtaatgg tattgctatt cctcatggaa aaagtaatgc tgtcaaaaaa
    18181 tcttctataa tatttgcaaa gactaagcat aagattgaat gggatgcttt agatgaaaaa
    18241 ccagtagata gtattttctt attggctata agcaatattg ataaggatag caatcatctt
    18301 gtactattat ctaaattagc aactaaactt atggatgatg acaatgtaga tgcactaaaa
    18361 ttagccaatt cagaacaaga aataatcaat atatttagtg aagaaggaga gatgtagaaa
    18421 tgaaaagaaa aattatagca gttacagcat gtgcaacagg agttgcacat acatatatgg
    18481 cagctcaagc gttaaagaaa gcttcaaaaa agatgggtca ccttataaaa gttgaaacac
    18541 aaggagctac aggaatcgat aatgagttaa ctacaaagga tgcacaaatt gcagaagtag
    18601 taatatttgc agtagatact aaagtcagaa atgctgaacg ttttgaggga aaagaaatac
    18661 tagaagtacc agttaatgca cctataaagg atgcagagaa agtaataaat gatgcactaa
    18721 agttaattga cactaaataa atggagggaa atataatgtt gaaaaagata gtctctgagt
    18781 taaaaaagca tgtcttaact ggaatctcat atatgattcc attagtaatt gcaggtgcta
    18841 tgataatggc gatatctcgt gtaggtggtt ctttctataa tataccagat atatgggatg
    18901 ctaaatatgc agaaagtgct agtagtattg ttagattatt acatgattta gatgggtttg
    18961 gaggtactgc attaggatta atgtttcctg taattgctgc atttatagga ttttctatag
    19021 ctgataaatt agctattgta ccaggtcttg ttggaggtat gattgctaaa gatataggtg
    19081 caggattttt aggagctttg gcagttggtc ttatagcagg atatacatgt ttgtttatta
    19141 aaaatcatgt caaattacca aaatcagcag cttctatagt accaatattt atagtaccag
    19201 tatttgggac attaatcact gttgtattaa tcaattatgt gataggtata ccttttgcca
    19261 cattaaatac tggattagaa aattggttaa atggattatc tggtacaaat cagatattaa
    19321 tggcagctat tataggggct atgatggcag ttgacttagg tggaccagtt aataaagcag
    19381 ctttaacaac atcacttgca ttattaacaa gtggaattta tgcgcctaac actgctgcaa
    19441 tggtaggaat agttatacca ccactaggat tgggacttgc tactatacta gcaaaacaaa
    19501 aatataataa acagttacgt gaagctggga aatcatctct tataatgggt ttaattggag
    19561 taagtgaagg agcaattcct tttgcagtag aatctccatt aaaagtaata ccttcatgtg
    19621 taattggaac agcaattgct tcagctatgg cagttggttt aggttcagta aatgcaactc
    19681 caattagtgg attttatgga tggtttacag tagagaattg gccaatgtat gtgttatcaa
    19741 tagcagttgg aacagtaatt gtagctggat tagttatagt attgcgtgga aatccagtga
    19801 ttgaggatga tgaatttgaa gatgatgatt ttgatgaagc agagtgggaa tcttaaaatt
    19861 taataactat ttaagacgtg ttataagcta tattcaatat gaatcttata ctaaataaag
    19921 tgttaaatat tcagtttaga cttaaatata aaagataaag aacagaggag aataaatgat
    19981 aaaagcacat attgttaatc atacacactg ggatagagaa tggtatttta cttctggaga
    20041 tgcattagta cttagtgaac aactatttac agatgtaatt gatgaattag aacgtaatcc
    20101 agatgtaagt tttgtacttg atggacaact ttctattttg gatgattatg tacaattaca
    20161 caaagaaaaa atagaaatca ttaaaaagtt aataaaagaa gatagactac atattggacc
    20221 atggtttact caaactgatg cattctttgc tagaggagag tctattttaa gaaacttaat
    20281 gattggaatt tttgaaagta aaaagtatgg taagtatatg aaaattgggt atttgccaga
    20341 tacatttgga tttaatgcac agatgcctat actattaaag catgtaggtc ttgataatat
    20401 attcttttgg agaggtatac atcttggtaa acaggttcaa tcaccatatt ttaagtggaa
    20461 aagcttaaat ggagaaagtt atatatatgc agtaaatatg ccacatggat atggaactgg
    20521 tatgctttta gagccttcat taaagtatgt taatggacgc ttagacccag ctattgattt
    20581 tattaaatca tatactgatg tagatgaggt gttaatacct tctgggaatg accagttaaa
    20641 tataataggt gatttcaaaa ataaaattaa agcgattaat gatatcggaa agtattcata
    20701 tataacgagt tcatatcaag agtttttaaa atatataaag tccatagaat cactagaaag
    20761 ctatagaggt gaatttagag aacctgtatt agcaagaata cataaatcaa ttggttctag
    20821 tagaatggat ataaaattag catgtgataa actagaaaac aagctgatta aaagaataga
    20881 gccattatta gtaattgcta aaaagagtaa aattgaaata agcaatcagt tacttataaa
    20941 tacatggaaa aaacttttgg aaggacaagc acacgatagt ttagctggat gtgtaactga
    21001 tactgtaaca gatgatattc ttcaccgtat aagagaagca aatgaaatat gtgacagtat
    21061 tgaaaatact attgtaaaaa agatttcaga agggcttaaa ttatctaaaa atgatatttt
    21121 agtatttaat acagaagcta agcgttttaa tggatataaa gagatacaag ttgtaagtga
    21181 tagtaaaaat atttatttta aagataattt agatgctact ataattgaag aaacttatgt
    21241 aaaaccgaga gaaaatgtat tagaagaaac acctgctggt aatatattta ttgaagaacc
    21301 aggatattat atattaaaag taagaataaa tatacagctt cctgcacttg gttataaagt
    21361 tgttagtttt gaattaagtg ataaagaaat ggtgtcactt gataaatcta atgacacttt
    21421 tatatctaat gataattata agataatata tgaagatgga agcttaaatt tacaacttaa
    21481 agatggaact tacatatcaa actttttaac attaaaagat agtggtaatg caggtgatac
    21541 ctatgacttt tctccactaa aaaatgatga ggatataaaa ctttcgtttg ataaagtgta
    21601 tacagaaaaa gctttaggat atgaaaaaat ggtggttaaa ggtactactt tactaccact
    21661 tacattagaa gatagaaaaa acaaatatct taaagggaaa ttaaatgtaa aagtaagtat
    21721 tgtcttatct aaagaaaacc cattgatgga tgtaaaactt tgtgttgata acacgatata
    21781 taatcataga ctacgtgttc atataaaaac tgatataaaa gataataaaa acatagcatc
    21841 attgccattt ggatatataa caagaaaaaa tggagtttta gacaactggg aagatattta
    21901 cagtgaaatg cctatagatt tagaaccttt ggaaagtaat attactctaa caaaccaaaa
    21961 tagaagttgt actgtattta ctagaggaat aaaagagtat caacatattg aagatgaaat
    22021 tgcattgacc ttacttgcta ctacagatga actaggaaag cctgatttat tatatagacc
    22081 aggacgtgct tctggtgata caactaaaaa aggtcatata agaattaaaa cagataaagc
    22141 tcaaatgctt aaggaattag aattttcatt tgcaatatat atggattcta aatcatttga
    22201 tgaattggaa attgcgaatt taacccacaa ttatctaagg gaatgtgtaa attatcaatt
    22261 acaggattac aatttctttt tatatcgtat agataataag attcaaaaat caattgtcaa
    22321 taaaactgta agtagagaat tagaaataat atctttacca gaaaactatc ttatatcagc
    22381 atgttatcct tcttattaca gtaaagataa atttataata agagtggaaa atccaagtag
    22441 taatagaatg atattggatg agcaaatatt taaaaataga aatgggaaaa ttgtaaatgc
    22501 aatagaagat gtagaagagg aacaggtata tgaaatagca ccttttgacg ttatttcaat
    22561 attattggat ttataagtgt aaaagtaagt ttattgttta aagtctgaca gatattaatc
    22621 tgccagactt tttaatttgt ataaatttgg gtaaatatta atgaatggtt ttagtattta
    22681 agtatgaatt tagtatttaa tatagaattt attgaagtag aat
//
LOCUS       DAELNY010000046        22475 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.046, whole genome shotgun sequence.
ACCESSION   DAELNY010000046 DAELNY010000000
VERSION     DAELNY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.046"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            292..1125
                     /locus_tag="KPE55_001550"
     CDS             292..1125
                     /locus_tag="KPE55_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0492639.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1218..2150
                     /locus_tag="KPE55_001551"
     CDS             1218..2150
                     /locus_tag="KPE55_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0492640.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2202..2684
                     /locus_tag="KPE55_001552"
     CDS             2202..2684
                     /locus_tag="KPE55_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0492641.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2696..3565
                     /locus_tag="KPE55_001553"
     CDS             2696..3565
                     /locus_tag="KPE55_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0492642.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3608..4363
                     /locus_tag="KPE55_001554"
     CDS             3608..4363
                     /locus_tag="KPE55_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0492643.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNTKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4405..5226
                     /locus_tag="KPE55_001555"
     CDS             4405..5226
                     /locus_tag="KPE55_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBG0492644.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDKNSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5231..5992
                     /locus_tag="KPE55_001556"
     CDS             5231..5992
                     /locus_tag="KPE55_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG0492645.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6153..6410
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6523..6762
                     /locus_tag="KPE55_001557"
     CDS             6523..6762
                     /locus_tag="KPE55_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0492646.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7066..8061
                     /gene="ilvC"
                     /locus_tag="KPE55_001558"
     CDS             7066..8061
                     /gene="ilvC"
                     /locus_tag="KPE55_001558"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG0492647.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8114..9802
                     /gene="ilvB"
                     /locus_tag="KPE55_001559"
     CDS             8114..9802
                     /gene="ilvB"
                     /locus_tag="KPE55_001559"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG0492648.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10013..10690
                     /locus_tag="KPE55_001560"
     CDS             10013..10690
                     /locus_tag="KPE55_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0492649.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10864..12900
                     /locus_tag="KPE55_001561"
     CDS             10864..12900
                     /locus_tag="KPE55_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG0492650.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13091..13657)
                     /locus_tag="KPE55_001562"
     CDS             complement(13091..13657)
                     /locus_tag="KPE55_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0492651.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            13945..14880
                     /locus_tag="KPE55_001563"
     CDS             13945..14880
                     /locus_tag="KPE55_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0492652.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15351..16565
                     /locus_tag="KPE55_001564"
     CDS             15351..16565
                     /locus_tag="KPE55_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492653.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(16985..17506)
                     /locus_tag="KPE55_001565"
     CDS             complement(16985..17506)
                     /locus_tag="KPE55_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492654.1"
                     /translation="MKFEWLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            17862..18659
                     /locus_tag="KPE55_001566"
     CDS             17862..18659
                     /locus_tag="KPE55_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0492655.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            18769..19359
                     /locus_tag="KPE55_001567"
     CDS             18769..19359
                     /locus_tag="KPE55_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG0492656.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19425..21122
                     /locus_tag="KPE55_001568"
     CDS             19425..21122
                     /locus_tag="KPE55_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0492657.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21336..22175
                     /locus_tag="KPE55_001569"
     CDS             21336..22175
                     /locus_tag="KPE55_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG0492658.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         9177 a         2207 c         3861 g         7230 t
ORIGIN      
        1 tatttgttta atatttctaa tttataaaaa aaaaattata aattttaaaa attataaatg
       61 acattttaac tagaattaat tgtaaaaaac actatataat actcaatttt caacattcta
      121 tacaattata cagaaattat acagcataat aatcaccgaa ataacaaatt ttaaaaatta
      181 agcaaaactt tttgtcgaag ttcaacgtct atatatatgt agtgtaaatt tttgggataa
      241 aatactacaa aaatataaat gtaaaaatat agtaatattg gaggtcgaac gatgagaaat
      301 aaaaagaaaa aaataaatag aaagaagttg tatttgcttt taagtgctat tgtagtgtgt
      361 ctggctttta tagtagttgg tgttcgcgtc agctctttaa atatgaagga aaatgaaatt
      421 agtagaaatg caaaactgtc aagttctact attacagata tagtatctaa tacatcaata
      481 gaagttagta aagggccaag taaaagttct ggaaaaatag catacataac tatagatgat
      541 ggtccatcta aatttactga cgagatgata aaaactttaa ataaatacaa tgttaaagca
      601 acttttttta tgatagatgg gaatatgaag gaatatccac agcaagttaa aaatataatt
      661 aaaaatggga acacagcagg ttttcatagt gtatcacatg atattcacaa gctttatgtt
      721 actagtacat cagcaaaaga ggaatttgat acaaatgatc aaacttttta taagataact
      781 ggtaaacatt caaaagtaat aagaatacct tatggaagca aaccatatac accacaagcc
      841 tcttatcagg ctttagttga tgctggatat aaaatttggg actgggactt agatacagaa
      901 gactggagat ctaattcttc tcaaattgta caaaatgtaa aaaatcatat taagaataga
      961 aaaggtgaag ataaagacca acttgtagtt ttaatgcatg agaaaaaaca gagtgcagaa
     1021 gctttagatt cagttttaaa attcttatca gatgaaggtt atgaatttgc gccagtagat
     1081 caaaaccaaa taccaaaaaa ttattggtta cgtaatttag agtagttaaa atataagaat
     1141 attgattgat atttttagaa attgagaata aaaatgagtg aggtgtaggt atattgaata
     1201 aaaaacttta tataggaatg gtcggaatat tgtctttgat gatggttggt tgtaataaaa
     1261 gtttagcaaa agtaaatgat gtggaaatca caaaagaaca gtacaaaaaa acaaaagcag
     1321 ttttatctgc tactaataat tatataaatg ggcagtcttt agatgaatta gaaaaaactt
     1381 tggataaaaa aggtagaaat aaattagaaa atgttataat atcgtttatg gtggataatg
     1441 aacttttata tcaagaggct aaggataaag gactaactcc aagtaaaagt gaagtagact
     1501 caaaatatca agaactagaa gataagatga atttgaatac aagctataag gaaaaaatgg
     1561 acaaagcagg tgttgataaa gattatttaa aacaagaaat atctagagat ttagcaatag
     1621 ataaaaataa aaaggccttt gaagatagaa taaacataag tgataatgat atggaagcct
     1681 actatacaag tcataaaaaa gattttaatg tagaagaagt aagtgcttct cagatactta
     1741 tatcgacttt agataaaaat aaaaaagaag taagcaaaga taaaaaagaa gctttgaaga
     1801 agaaggcaga taatatattg actaaaatca aaaatggaga aagttttgaa agtttggcta
     1861 aaaaatattc ggatgataaa gcaactggaa aaaatggagg acagctagga tattttacaa
     1921 aggatgataa aaatgctgag tttacaaagg aagtgttcaa attaaagaaa aatgaagttt
     1981 caaatgtgtt tgaaactagt tatggatatc atatagtcaa agttactgat aaaagagaaa
     2041 gacaaaaaag ttttaatgag tgtcagagtt taataagaga atctatttta aatgaaaagt
     2101 atattgagca tataaaaaaa ctgaatgaag atgctaaaat agacagatag aatttctaac
     2161 agctcaatcc tagcctgtaa tcggagggag attttacagg aatgtttaag agtttaaaga
     2221 gaacgaaagt tgagaaatac attatcgata ataaagactc tttttataga atagcatata
     2281 gttatacgaa gaatgaagaa gatgccttag atgtggttca agaagccatg tataaggcac
     2341 tatactcagt ggaaaatatt aaagaagtaa attatataaa gacatggttt tataaaattt
     2401 tagttagaac gagtatagat tttattagaa aaaaccgtaa atataataat atgacagata
     2461 ttgaccttat agatgagact ggagagtatg ataaatatac tgatttggat ttaagaagag
     2521 ctttggaaga attacctatt gaatacaaat ctattataat attaagattt tttgaagatt
     2581 taaagattga agaagtagca ataatattgg atgaaaatgt gaatactgtt aaaactaggt
     2641 tatatactgc tttaaaaaaa ctaaaattaa aaattgaaga gtagggataa aaagaatgac
     2701 aagcaaagaa aggttaaata agttaaaaga tgagtatcat aaaattccaa tacccaagaa
     2761 attagatact ataataaatc atgaaaaaat agaaaacata tatcgagaga aaaataaaaa
     2821 agtaaataga ttaagattta aagttgcaat agcctttgca tgtatattta cagtattagt
     2881 aaatataagt cctgtatttg cagacaattt ttctaagata cctgtaattg gagcaatagt
     2941 agaagtaata actataaaaa attatagttt aaaaagtgaa aattatgaag ctgagattga
     3001 tattcctaaa atacgaggtt taaaagataa aaacttagaa caaagattaa acagtagttt
     3061 tatggaagat ggaaaaaggc tttatcatca attccaagag agaatggaga agatacaatc
     3121 atcaaagaat aaaggatata aatctttaag tttgagttat agtgtaaaaa ataattctaa
     3181 aaaatttcta tcaatagaga tgacaaaaaa tgagatagag gcttcatcat atgtgagtaa
     3241 ggtacattat actattgata aaaaaagaca aatagttttg actttgccaa tgctttttaa
     3301 agatgataag tatataaaag ttataagtga taatataaaa gaacaaatga gagaacaaat
     3361 gaaaaaggat agtacaaaat cctattttat agatcaaaaa aaagatttgc ctgttgaaga
     3421 ttttaaaaca attaataagt atcaggattt ttattttaat aaaaatgaag accttgtaat
     3481 ttgttttgat gaatatgaag tagcacctgg ttatatgggt gcagttgagt ttgtaatacc
     3541 atataaagta ataaaagatt tgtaaattga atagtagttt atatataaat attttaagaa
     3601 ggacaatatg actacatata aaaagcttaa aaaatctata tttaaaattg ctatattttc
     3661 aatatgctta ttagtttcat tagtagatac aaaccctata tttgcaggta gttcctttaa
     3721 tgaaacttca aatagtgata cgataaaggt gttaaatact aagagtatca actttaaaca
     3781 agacaattat gaagtgaatg tgagtattcc taaaataaaa ggtttaaaag ataaaacttt
     3841 agagaataaa ttaaacaatg agtttttgca agatgggaaa aaaatttaca gtgaatttca
     3901 aaataaaatg aaaaaggaag ataataaggg tcatagatac ctaaatgtaa tccacaatgt
     3961 caaaaataat tctaaggact ttttatcaat agaaatgaca aaagaagaga tagaagcttc
     4021 ctcatctata actaagaagc attacactgt tgataaaaat agaggaatac tattaaattt
     4081 accaatattt tttaaagata gtagatatat tagagctata agtgataata taaaagagca
     4141 gatgagaaat caaattaaga aagatgatac aaaatcttat tttatagacc aagataaaga
     4201 ctcgccagtg aaagattttg aatctataaa taagtatcaa gatttttatt ttaataaaaa
     4261 tggaaatctt gtgattagtt ttgatgaata tgaagtagca cctggttata tgggagcagt
     4321 agaatttgtg ataccaaata aagtaataaa aagactaaga tagatatata gaagattttt
     4381 agatagactg gtaaggagaa ttatatgaat atatatgaac aagcaataag attaatagaa
     4441 aataatgagg atttcgcatt tgctacaata acgtctcatt caggttcaac accacgtgaa
     4501 actggagcaa tgatgattgt gaaaaatgat tctactatat ttggctcagt aggaggaggt
     4561 agtgtagaag cagaatgtat aaaacatgca attaatgtta taaaaaacag agaatcaatg
     4621 ttgtataaat ttactctaaa caaatctgat gtggcaaaac taggaatgat atgtggaggt
     4681 actggagaaa tacaaataga ttttatagat agtaaattaa aaagtaatat agaaaaattt
     4741 aataagagac taaaggaaaa tacaagtaaa gcatatatat ttggtgcagg tcatatttca
     4801 agagatgttg ctgtgatact atctctttta gaatttagaa cagtagtaat agatgataga
     4861 gaagaatttg ctaatcatga aaggtttcca gaatcagaag tagtagtttt agattctttt
     4921 gaaaacatac cagattttcc aactgacaaa aatagttata taattatttt aacaagaggt
     4981 catctatatg attcaagtgc tttagaatgg gctcttaaaa gagaggctgg ttatataggt
     5041 atgattggaa gtagaacaaa aattggattg acttacgaaa aactcatgaa aaaaggtttt
     5101 aagaaggaag aattatcaaa agtacatgca ccaataggca taaaattgaa tgctcaaaca
     5161 ccagctgaaa tagctgtatg tatagctgct gaacttataa attgtagagc taataaggaa
     5221 aaataaacta atgaacacag ggagtaaaga tattgatact ttaaaggtta attttagagg
     5281 gattgaaata aaagcagttt tagtatataa aaatagaaaa aatatttcta taaaaataga
     5341 tccatttggc aaaataactg taatgagtcc tcctaaaata tccaaaaaaa taataaaaga
     5401 tataattatt gaaaaaggag actggatact taaaaagtca gaaaaatgta aaggaagaga
     5461 ggaagtatat aaacaaagaa tgtttataac tggcgaaaaa tttctttatt taggtgaaga
     5521 gtaccattta gttataaaag aactgttgag agattctgtc aaaaaaagta actgtagaat
     5581 aactattaac gaatatcaaa tagttgttca gacaaatgat acaagtacag attttattaa
     5641 aaaatcttta aaatcttggt ataagatgga aagtgaaaga atagtattag agagaataga
     5701 ttttcttaaa ttaaattgtg aaataatgaa gcaattagtt ccagcgagtg ttaaggtaaa
     5761 agaacaaaat aagagatggg gaagttgtac agctcaaaaa aatatttata taaactcaaa
     5821 aatatctatg gcaagattag atgtaattga ttatgtaata atacatgagt ttagccattt
     5881 agtacatatg aatcactcta aaaaattcta tgatttagta aaatccatta tgcccgatta
     5941 taaagataaa gaaaattggc taaaaataaa tggatataaa ataataatct gaaaacgatt
     6001 attccaaaat ataggcgtaa agtataaaag aagtattatt gtgaaaaaaa taaataatct
     6061 gaaaatttta aaaaaagtca ttgacattgt aaatgatagg gaatataatc tatattaaca
     6121 acaaaacaga attaaaatat aaaattaaaa aattaaaagc aatgacagag acaacaatac
     6181 taatagtgtt atccacagag aaccaatgtt gctgagaatt ggtgttaaca gtagtataat
     6241 gatcactctg gagcttttgt actgaatatt tttagtaagt acaaacgtat gtcagcgtta
     6301 catgactaag gttaaggtaa ttaattaccc gacctgaaaa aagaggggta gatgtatttt
     6361 ttatatctac aaataaggtg gtaccgcgga atataacttt cgtccttata agtaatattt
     6421 atattgctta taaagatgaa agttttttta ttttaagcga tataaataaa tttaatccat
     6481 ataaaattaa taaaaaaata ataaaaataa ggagggattt ttatggaaaa tactgtatat
     6541 gctaaattaa accaaggaat agattctttt ttaagagtgg ctatgactct aagaagaaga
     6601 gaggttgata tacaatcaat aagtatgact gtagataata tgaataattc aggtataaaa
     6661 ttaatagtaa atgaagaaaa gacatcctta gattctgtct taaaccatat gaaaaaacta
     6721 catgatataa gagagataac tgttgaaaga atgagacatt aattaaatat attttataaa
     6781 gctaaagata aatatgctta ttttaaaata aaaaactaaa gataaatatt aaataagaaa
     6841 aatcttagat taaaatataa gtgtcattga atttattagt ttatcacagt taataaattt
     6901 attagtttgt tgtagctaat aaaaactgaa aattctaaaa ttataattgt aagattatat
     6961 aaatttagaa tttaaatctg ttgaataata ttaaaacaat gataatgagt atgaatttct
     7021 aataaaaata aatatcataa aaataagaaa aagtattggg aggaaatgaa aatggcaaga
     7081 atgtattatg aaaaagatgt ggatttagaa gttttaaaaa ataaaaaggt tgcagtttta
     7141 ggttatggaa gtcaaggaca tgcacatgca caaaatctta gagataacgg tgtacatgta
     7201 gtgataggtc tttatgacgg aagtaaatca gcacaaaaag caaaagaaga tggatttgaa
     7261 gttaagagtg tagcagaagc tacaaaagaa agtgatttga caatgatgtt aatgcctgat
     7321 gaaaaacaaa agaaagttta tgaagagagt gtaaaggata acttaaaaga aggccagaca
     7381 ttagcttttg ctcatggatt caatatacat tataatcaag tacaaccacc agaatttgtt
     7441 gatgttgtaa tggtagctcc taaaggacct ggtcacttag ttagaaatgt atttacaaag
     7501 ggaagtggag ttcctgcact atttgcagta tatcaagact acactaaaaa agctacagag
     7561 acagttttag catatgcaaa agggatagga gcaacaagag caggtgtttt agaaactact
     7621 tttaaagaag aaacagaaac agatttattt ggagaacaat cagtactttg tggtggaata
     7681 agtgaactta taaaattggg atataaaacg cttgtagatg caggttatca aaaagaagta
     7741 gcatattttg aatgtttaca tgaaatgaaa ctaatagtcg accttatata tgaaggtgga
     7801 tttgaaagaa tgagatatag tataagtgac acagcagaat atggagatta tgtatctggt
     7861 aaaagggtaa taactgatgt tgcaaaacaa ggtatgcaaa atgttttaga ggatatacaa
     7921 aatggaaaat ttgcaaaagc gtggattaaa gaaaatgaag aaggacgtga aaacttccta
     7981 aaaactagag aagaagaata taatacagaa attgccgaag ttggtagaaa tctaagaagt
     8041 atgatgtcat tcttaaaata aatatatatt aaatggatta gtttgaggga aagagtggtg
     8101 tatagtagtg cgtatgaatg gagcaaaggt aattttagaa tgtttaaaga aagagggaat
     8161 tgacacaata tttggatatc ctggtggagc cgtgatacct ctttatgatg ctctatacga
     8221 ttattcagat gattttaaac atataaggac atcccatgaa caaggtttag ttcatgctgc
     8281 agatgggtac gcaagaagta ctaatacagt aggagtttgt tttacaacat ctggtccagg
     8341 agcaacaaat gccataactg gaatagcaac agcttttatg gattcttcac ctatggttgt
     8401 aatttcaggg caggtgccaa ctagtttatt aggaaaagat tcttttcaag aaattgacat
     8461 aactggagca acattatcta tgacaaaaca taattattta gttagaaaca ctaaagaatt
     8521 agttcctaca ataaaagaag cttttagagt tgcaaattca ggtagaaaag gacctgtact
     8581 tgtagatgtt ccaaaagatt tatttttagc agaaatggat tttagtggtg aggactatga
     8641 tttatgtcaa atagatgatt atatggatta taagagtgat tttgatctag atgatgaaac
     8701 taatattaag cttttaaatg aagcaataga tatcataaaa gaatctaaaa agcctataat
     8761 atatgcaggt ggaggtgtta aatcttcaga cagtgaggaa attcttgaaa aatttgcaac
     8821 taagatagat acacccgtac taaatacact tatggggctt ggaaatatag atagaaagaa
     8881 tgaattatct cttggaatgg ttggaatgca tggaagtaga gaaagtaatt tagcactttc
     8941 taattcagat ttagtaatag cgataggtgc gagatttagt gatagagtga taagtaaaag
     9001 ttctgaattt gctaaaaatg caaaaataat acacatagac atagacccat ctgaaataag
     9061 taaaaatata gaatctaatg tatctttggt tggagatgta aagctagtcc ttagtttatt
     9121 aattgaaagg gttgaaagta agaataatag caattggaaa gaagagataa agagatttag
     9181 aaagagtgag ggtgtacaaa catatgaatt tcatccacaa aatatactta aaaaaataaa
     9241 tgaaaaatat gaaacattaa agaaacccac tgtggtagta actgatgtcg gtcaacatca
     9301 aatgtgggct gctaagtact ggaattttaa aggtaataaa agttttataa catcagcagg
     9361 gttaggaact atgggatttg gtttaggtgc agctattgga actaaagttg gtaatgtaga
     9421 taaaaatgtg gttttagtca ctggtgatgg aagttttaga atgaactgta atgaactagc
     9481 tacagttgca aattataatg tacctatgct tattttacta ctcaataata gaacattagg
     9541 aatggtaagg caatggcaaa agttattttc caatcaaaga tactctcaaa ctgacattaa
     9601 tgaaaatgtg gattatgtta aactcgtaaa tgcatataac attgatggat ataaggtatc
     9661 cagtatggaa gaattaggaa aggcgctaga tatgatagat tttaataaac cagtattttt
     9721 gcaatgtgat atagataagg attatgatgt ttatcctatt gtagcaccta atgatgcatt
     9781 ggaaaatttg atatgtaact aaaacttata ctgaatattg ataaaatttt ttattaaatt
     9841 tttattagta ttcagttttt gttgtttaga gatagattgg cattttttat ttttgcaaat
     9901 aaatcaatga ctatataatt aagaacttat taaattttaa ttatacaatt tagaatagta
     9961 ttacaactta agtaaataat aattttgaat aataatttac ggaggtattc atatgtttaa
    10021 acacgataaa gcattattaa aagaagtaaa agtagaaaga ccaaatcctc aatatgctgt
    10081 tttaatgcaa gaacagttgg gtggtgcaaa tggagagtta aaagcagcta tgcaatactt
    10141 gtcccaaagt tttagaataa aagaccctca aataaaagat ttatttcttg atatagcagc
    10201 agaagagctt agccatatgg aaatggtagc acaaactata aatttattaa atggacatga
    10261 tgttgattat aatgcagtta atacaggcga aatagaaaca catgtattaa ctggtctatc
    10321 accagtctta ataaactcat caggagctcc ttggactgct aattacgtta cagttacagg
    10381 ggatttagta gctgacttac tttcaaatat agcttctgaa caaagagcta aggtagttta
    10441 tgagtacttg tatagacaaa tagatgataa atatgtaaaa gaaacaatag atttcttact
    10501 taatagagaa gaagcacata atgctctatt tagagatgct ttaaataaag ttaaagatac
    10561 aggttcaaat agagattttg gagttacaga agattctaaa ttatatttcg atttgtctac
    10621 tccagggcca aatcacgata ctaagataga tattaatcct ccttcttttg aaaaaccttt
    10681 aaaaaaataa tttgatattt tttatgttgt tttgttttag tagttataaa aattatttac
    10741 tagaagaatt aattatatga aaaattaaat atgtatagtt aaaaatgggt atacacttat
    10801 tataagtgta tatctatttg tttattgcaa aacatataat aaaataacaa atgaggtggt
    10861 attttggaca gtaatagaaa acctaataac ctaataaatg aaaaatctcc atatctttta
    10921 cagcatgctt ataatcctat aaactggtat agttggaatg atgaggcttt taaaaaagca
    10981 aaagaagagg ataaaccaat atttttaagt gtaggatatt caacatgtca ttggtgccat
    11041 gttatggaaa aagaatcttt tgaagatgaa gaagttgctg aaataatgaa tagaaacttt
    11101 gtggcaataa aagtagataa agaggaaaga cctgatgtag atagcgtgta tatgacagtt
    11161 tgccaagcta tgacaggtag tggtggatgg ccaatgacca taataatgac accagataaa
    11221 aaaccttttt ttgcgggaac atatttccca aaatattcaa ggtataatag gcctggagtt
    11281 atagatttat tagagaatgt atctgaaaag tggaatacaa gcagggatat attaataaaa
    11341 tctggagatg aaataataga agctcttaag gatgattttg gtgtaaaaaa tactgagggt
    11401 gatttatcaa aagagatgtt gagttcatca gttagagtat ttaaggctat ttatgatgaa
    11461 aagtatggtg gttttggtaa tgctcctaaa tttccaagtc ctcagaatct tatgttttta
    11521 atgaaatatt atagtataga aaaagacaaa gatgtgttaa aaatggtgga aaaaacttta
    11581 gatggaatgt atagaggagg cttgtttgat catattggat ttgggttttc aagatactct
    11641 actgataaaa agtggttggc tcctcatttt gaaaaaatgc tatatgataa tgctatgctt
    11701 actatagctt ttttagatgc gtataaaata acaaagaaag agttgtataa agaaattgct
    11761 ataaaaacta tagattatgt agttagagag atgaaggata aagaaggtgg gttttattca
    11821 gcacaggatg ctgatagtga aggtgaagaa ggtaaatttt atacatttaa tcctcttgag
    11881 ataattgaag ttttgggtga agaagatgga actttcttta ataattattt tgatataact
    11941 agtagtggaa attttgaagg caaaagtatt cctaatctta taaaaaataa agaatatgag
    12001 agacataatg aaaagatagc tgaccttagt aaaaaggtat ttgaatatag aaaagaaaga
    12061 acatctttgc ataaagatga caagatactt acatcatgga atgcattgat gatagttgct
    12121 ttaacaaagg catatagtac tttaaaaaat gacatatatt tagagtattc aaataagtgt
    12181 cttaacttta taaataataa tcttgtaaat gagtcaggta gattattagc tagatataga
    12241 gatggaagtt ccgactattt agcatattta gatgattatg cttttttaat ctgggcgtat
    12301 atagagcttt atgagtcaac atttaatatg aaatatttag aaaaggcttt aaatttgaat
    12361 gaaagctgta tcaacctttt ttgggattat gaaaaaagtg gattttatat atatggtaaa
    12421 gatagtgaaa atttaatagc aagaccaaaa gatttgtatg atggagctat tccatcagga
    12481 aattctgttc aattatacaa tcttataaga cttgcgaaaa ttactggaga taatagactt
    12541 gaagaaatgt catataaaca attaaaactg tatgtggata atgtaaagag ttctccaact
    12601 ggctacagct tttatatgct ttcattgatg tttgaacttt attctactaa ggaaattata
    12661 tgtattttta aggaagattc tgatttaatt gcatttaaag aattgataag tgaaaatttt
    12721 ataccaaatg caactttttt agcaaagaaa tacaatgaag aaaacactat tataggtttt
    12781 ttaaataact atagattgaa ggatgataag attagttatt acgtgtgtca aagtaatagt
    12841 tgtagtcaac caattaatga tttacaaaaa ttaaaagata tgattttagg agtagaataa
    12901 tcataatagc gaaaaaggag atgctttaaa ataaaagtct aatcatctcc tttttttgta
    12961 caaaaaacag aaaaagagtt cctaagaaca cctaatctat gtaatgattg ttttgttaaa
    13021 aaataaaaat gacattatag ttatataaaa tattgtacta taaaaaagtg gtattataga
    13081 tgcgatagta ttaatctttt ttatttactc tatttcttct ttttgcaaat tcttcaagtg
    13141 cttcagattt aattttatct atttcttcat attccataaa attagcatct tcttcaaagc
    13201 catcttgttc ttcaccatta aaacgataca ttttttttag taaactccat aaggtctcta
    13261 attcatcttt ggtaaattcg tgaaatacat ctgccataaa gtttattcca gtcctactac
    13321 atgtcaccat aacctttttt cctaagtctg taactttaac attaattgct ctcttatcat
    13381 gaggacttgg aattacatca acatatccat ttttttcaag attagcaact agtctattta
    13441 tattttgttt acttgtaccc atttttcttg caatattatt taatgttgtt tcttcctctg
    13501 gtaaatgaag aattgataaa atagtcatat actgtcttga agtaagtata ccaaaatatt
    13561 tatctccttc tatttgtatt ttgtttgtaa gggtaaaaag agttgcatat gtttgattca
    13621 ttaaaaagag ctcttttagt tcatttgaat aatccatgaa atatatctcc tttattttta
    13681 gatgttgttt tgtcgaaaac agaatatcat gaattaattc taatgtcaat atggtgtcaa
    13741 aatatattaa agtattactt ttttatgtaa ctattaatta attaaattat tgtataattt
    13801 tatgtaaaaa cttctatgta ccaatgataa cttctatgag gaattaataa ttcttaaatt
    13861 ttttagaaag attttgactt gaaaatactt gtaatataat atataaatta atatactgaa
    13921 taaaatcaat ctggagggat aattatgaag tgtacaaact ttactttcaa aggagaggaa
    13981 ggtttagata tatatacata taaatgggaa gatgaaaata taaaaaaacc aaaggcagtt
    14041 atccaaattg cacatggaat ggctgaaact gcacaaaggt atgagacttt tgccaaggta
    14101 cttactaaaa atggttatat agtatatatc aatgaccata gaggtcatgg gaaaactgca
    14161 aagataatag aaaatgttgg acatttagct gaaaaagaag gatttagatg tcttgtagag
    14221 gacatgtata ctttaacaaa tattataaag aaagaaaatg aagatctgcc aatttatcta
    14281 tttggacaca gcatgggttc atttgcaagt caaagatata taatggatta cagtaataat
    14341 ttatctggtc ttatactatg tggttcaaat ggaaagcaag gaatcattct aaatcttgct
    14401 catctgataa tcaatcgtga gattaaaaag tatggaagac gttctaaaag taataaaata
    14461 aataatttga tatttggcgg cgaaataata agaagaaatg aaaagactaa gtttgactgg
    14521 ttaagtagag ataaagaaca agttgaaaaa tatataaatg acccattttg tggagtagtg
    14581 tgtagttgtg gcttttttta tgatttagtt caagggttaa aagaaattga ggataaagaa
    14641 aacttaaaaa aagtgccact tgatatacct atttacataa tatctggaga taaagacccc
    14701 ataggaaaaa atggtaaagg agtcttaaga ctaagggata gatatataaa attaggagta
    14761 aaagatgtaa cttgcaaact ttacaaagat ggaaggcatg aattgttgaa tgaaataaat
    14821 agagaagaag tttttgagga tataatttgt tggttaaata ataaaataga aatattgtaa
    14881 taaaaaaata taatgatata tttgctttaa atttatttat atataatatt tgaaaagtgg
    14941 attatgtata tgcaaagtag aaaagagagc ccagaagtga ttttttgcta taatgccatt
    15001 tattgaatag gtgggtgtta ttataaataa attattaaca ctcagtatta ttatcattgt
    15061 atatggaatt tattttattt aaaattcctt gaaagtttaa aagtttaaat atagtaagtt
    15121 gtaataaaga gaaaatctaa atatttaaat gctatttaat gtgaatctat taattaggtt
    15181 atacttaaac ttaattaata gattttatat ttcaagccaa tgagataggg ttttagatta
    15241 aaatataacc aatggttaaa ttacagacat tttgttaact ttccctcgat ttactagtaa
    15301 atattttttt tatatactta aattacaaca gatgtactag ggaggtatat atgcaaatta
    15361 aaataggtaa agtcatacag tgtcttagaa aagaacgaaa tttaactcaa gaacaattag
    15421 caaagtttat aggtgtgtca acaccagcag tatctaagtg ggagagtgga aattcttacc
    15481 cagatataga attattgcct ttattagcag acttttttaa tgtgtctatt gataagcttt
    15541 taaattacaa gattgattta agtgaagaag aagtaatgaa aatttataaa gaattagaat
    15601 caggctttgc tagaattgaa attgatttat caacagaaga accaaaagaa gaatttagac
    15661 aagatttaga atctgtaaaa aagctttcca atatgtatat agagaagtat cctaaaagct
    15721 atttattgaa gctaaggata tgttcgctgt atcagatgta ttcatataaa tttggtaaga
    15781 gtgaacttaa tgacagagta aaagaaacaa cgaatattct tgaagatata gtgagaaata
    15841 cagatgatat ccaaataaaa gaaacagctc ttattatatt atcaaacgca tattgtatgt
    15901 tggaggatta tgaaaaggca gagttgtatt taaatatgat acataagtca ataggggata
    15961 caagtgttaa tttagctatg atatatttaa agcaaaatag acttgaagaa gcagaaatat
    16021 tgcttcaaaa taaattgttt agtaatgtat ttaatataag catggattgt aagggaataa
    16081 ttaatgtata caaaaatcaa tataaggaat taaagaaaaa attggagaat agaaatttta
    16141 ataaaaatgc aattgagaga gaaatggagt atataaaaaa taagttgctg gggtatgcaa
    16201 atctttcttt agaaataaaa aaaatgctca gtgaagataa aggtgcattt tttagtatgt
    16261 atatggatta tatggaatta tctctaattt ttttattttt taacatgaaa gaagaagcaa
    16321 aaaaagcttt atatagtcta aaagaaatat tagaaaaata tcctatacat gaaagtttgg
    16381 atgtaagtca aatgagattt tttgataagg tagagtctaa aaatttatat acttttaata
    16441 tatatacaaa cttgttaatt gtacttaatg atgacagtta taatgagctt agagaagaac
    16501 caatttttaa ggatgtaatt gaaaagattt tagatatgga aaaaatgttg aaaaataaag
    16561 aataagtaaa aatttaatat aggacttaaa gattaatcaa attaaaagaa agtgtttcaa
    16621 aataaaaatt tgaatcacct tttttgtcgg tcttaaacag ttatttagaa aaatagaggt
    16681 ttcaaaatag aaatctgaat tatctgtatt attacacaaa aatagaaacc taaatttata
    16741 aaaatagaat aaagtttgtt atataaaatg taattttgtc tataaaatct cacctgtttt
    16801 cttaacttta agcgtgaaaa agaggatgta tcatgaattt tttagttcat gatgcatcct
    16861 gctttgtatg aaatccaata tatctttatt atttaaacaa tggaaaagag tcttatttat
    16921 atttggagac atctctttta tttaattatg aagttttata cagttcaata aataggaatt
    16981 atatttagaa actcatgaca ttatttctta gatagtgatt aaatttatca tattccattt
    17041 tagataactt ttctgggtaa gacattacaa tcaggttatg cccattaaat tttttatggt
    17101 atggtggatg aatgtgttca taattatttg tcttatattt tactctttca tagaacgcct
    17161 tgcgataaat tgaaatctca tcaattggaa ggtcaatttc taagataata gtttttcctt
    17221 tcttatttaa aagctctaac gctctttgcc catatctatt attgcgcatt tctggaaaaa
    17281 tgcagaaatg ttctacatag ataaagtcat ctgtttccca acataaaatt atgccgacaa
    17341 actggttttt atcataaatt aaattgaatt gatattcttt atttttcaaa atttctgctt
    17401 gcaaagaaga ttttctttgt tcatgaaatg ggaaactaat tttatataat tccatagcct
    17461 tgtaatataa tttattttca ctggttctta accattcaaa tttcacttta ctcctcctaa
    17521 tcacgaaaat tccaatttat ttatgagttc attttaccat agtcagtatt tgcatacaat
    17581 gtgttttaag ttgagtgtca tttctttctt ttatttatgc ttatatctca tagtctaaga
    17641 gataattgct taagacataa ctaaaaacta ttatattaag ctttgataat ctaaaaacag
    17701 tatatttatg gacttttcta ggtctaaaag taatcttaat aattttagaa gttacaaaaa
    17761 agttacataa atttggaaag ctataatata catatataat ataaggttaa aactgtaatg
    17821 aataatatta atttaaaatt tgaacatgga aggagattat tatggagaaa agagaaaaga
    17881 ggataataat atcatcttta ttaagtgtat caattttaat ggggttggta agtatatatt
    17941 ctatattaaa taaagaagac ataattttaa ctgttaaggg tcaagagcaa aaagtatctt
    18001 cctttaagaa aacagttgag gaacttttgg atgaacaggg tgtaaagtat aattctgaag
    18061 ataagattaa tccaagttta gatacagaac taaaagatga tatgaaaata aaagttgtta
    18121 aggtaactaa aagtaaaaaa gaagagattg aaaaaattcc atttgataca aagcatgtaa
    18181 atgatagtaa tttgttaaaa ggaaaatcta aagtttatca agaaggtcaa gaaggagaaa
    18241 aaaaactagt ctataattta acttaccatg atggaaagtt agtcaaaaaa gtcttatcaa
    18301 aagaagtaat atctaaggag ccaactacaa aaattataaa atatggaact aaagaaaaag
    18361 tactaatagc atcaagagga gcaaatataa gaggaggcaa acatatgaaa gtggttgcca
    18421 ctgcatatgc aggagataca ataacatcta ctggtacaac tccaagatgg ggtgttattg
    18481 cagttgaccc acgtgtaata ccatatggaa caaaagtata tatacctaaa ctgggtatga
    18541 cttttgtagc agaagattgt ggaggtgcaa ttaaaggtaa tagaatagac atttttatga
    18601 acagtgaagg aaaagcttca aattggggaa ggaaaagtat agatatatat ctacattaaa
    18661 ttagtgaata gaataacttt acttgaagga caaaaaagta taaaatatga attaactaaa
    18721 ctgttagatt attcttaaaa cataaaaata tatctaaggg gggataagat gaaaatagtc
    18781 tgcttaggag acagtttaac gtatggattt ggagtatcac gaagtaattc atggacaaac
    18841 attgtaaata aagagactcg attagaaata gtaaataagg gtataaatgg tgatacaaca
    18901 agtggtatgt tagctaggtt taatgaagat gttgtaaaaa attctcctga tatagttttt
    18961 ataatgggtg gcaccaatga ttttattgct ggtgcgggaa atgaaattat taattctaat
    19021 ataatggcta tggtgcatca gtcatattca aaaaatatta tacctataat aggtatacca
    19081 ttaaaaccag atattccaaa tgttagggaa gattggagtt gttttactga ttttaatata
    19141 gtatctcaaa acttagaatc atatagttat tggataaaaa aattttgtat gacatttaat
    19201 actaatttcg tagattttta ttcagaatat aataaaaata tggaactaga aggatataaa
    19261 aaattatatt ttgatgggtt acatccaacc aaagagggtc atagaataat ggcagacatt
    19321 tttattaatt caattaataa gtatatagaa gagttataga ttacattaaa atgcttgttt
    19381 atgcgtcagt tgcaataaat acatacagta gagaggggtt tattatggat tatactaaaa
    19441 cttatgaaga gtggataaag ggttcatatt ttgatgaaga tacaaagcta gagttggaga
    19501 atataaaaaa taatgaaaaa gaaatagagg atagattcta taaggattta gaatttggga
    19561 ctgcgggtct tagaggaata attgaagcag gaacgaatag aataaataag tacacagtta
    19621 gaagagctac ttttggattg gcaaactata tattagaaaa cacaacaaaa gaagaaacaa
    19681 gtagaggagt agtaatagct catgataata gacacaagtc tagacaattc tgtatagaat
    19741 ctgcaaatac tttggcagca tgtggtataa aagcatatat atttgatagt ttgagaacta
    19801 ctccagaatt atcttttgct gttagaagct taaatgcaat cgctggtata gtcataacag
    19861 caagccataa tcctccagaa tataatggat ataaggtata ctgggaagat ggagcacaag
    19921 ttatgccaga gattgcaaat gcaataacag agaaagtaaa tagtatacat gattatagta
    19981 caataccaac cttaacggaa gctaataaaa atttggtagt tttgttggac gaatcacaag
    20041 atactaaatt tatagaagca gtaaaaagcc aaattataag aaaagattta gttaaaaatg
    20101 taggtaaaag ctttaagata gtttatacac ctctttgtgg aacagggaat gttccaataa
    20161 gaagagcatt gaaagaagtt ggttttgaaa atataattgt agttccagaa gaagaaaatc
    20221 cagacccaaa ttttgcaggg cttgattatc caaatccaga agagaaaaaa gctttaaata
    20281 gaggtatatt actcgcaaaa gaaaaaggag cagaccttgt aatagcaact gacccagatt
    20341 gtgatagagt aggtgtagca gtaaaaacta ctacaggtga gtatgcttta cttacaggaa
    20401 atcaaattgg tggtatgctg acacattata taatagaagg attaaaagaa aataataaat
    20461 taaaagaaaa tcctacaata ataaaaacca tagttacatc agaatttgga gcagatatag
    20521 ctaaagcaaa taatgttgat gtattaaatg ttcttacagg ttttaaattt attggtgaaa
    20581 aaataaaatt atttgaacaa aataaaaata gaagttatgt atttggatat gaagaaagtt
    20641 atggttactt agttgggact catgctagag ataaagatgg tgtagtttct tcattgttaa
    20701 tatctgaaat ggcagcattt tactattcaa aaggtatgag tttatatgat ggtttaatag
    20761 aactttataa aaagtatgga tttttcaaag aacagactat atctttgact ttaaaaggta
    20821 tagaaggtgt agaaaagata aaagaaataa tatcttattt tagagaaaat caaattgatt
    20881 atataaatag tattaaagta gttgataaaa aagattataa aaatggtata gataatcttc
    20941 ctaaatctga tgttttaaaa tactttttgg aagatgaatc ttgggtagct ataagaccat
    21001 ctggaacaga accaaaactt aaattttaca tagctgtaaa aggagctagt gatattgaag
    21061 cagataaaaa gattcaaggt ttgaaaaagt atatagatga catggtcgaa aaattaaaat
    21121 aaaatattat tattttataa gataattttg taataatatt tatttagctg aaaattataa
    21181 tttactttca ttgagtagaa tatggaaatg ttagtagaat tgttattttc agcttttatt
    21241 tgtgttattt ttacttaaat aattatatta tagaatgaat ctgagtataa ttaatattgt
    21301 ataatgaaat taatttgata gaaaagggga tatttatgtt tggtatagat aagaaattga
    21361 ttgaatatat aattgcaaaa tccaattaca gtgaattaag ttcaaagtca gcagaagtat
    21421 ctttcttctt aatgttgtcc atatttcctt ttttaatatt tacaataagt tctatagcct
    21481 atataccgat tcttcattta aataagtata tagccttgtt tagaaatatg atgccagaag
    21541 gtgcttttgc tgtgttatct tcaatcatag tttctgcaat agacaataga aacctgaaat
    21601 ttttagcagt cagttttgta cttactatgt ggacattttc aagagcagta aaagctctta
    21661 ttaaaggaat gaatagagcc tataaagtta aagaaactag gtcttttttt aaaattttat
    21721 ctatatcatt tttatttaca ataatgcttt tggtccttat ttttttatct atgatatttt
    21781 tagtttacgg ggaaaaaata ggttatttta tttttaatct tgtaggattg gatgagatat
    21841 ttataaaaat atgggatatt ctaagataca ctgtaggtat aataacaata atagtgatat
    21901 ttacactttt atataaatac actccaaata aaaagttaac tattaaagaa tctgcgccag
    21961 gtgctatttt tgctactttt gcttggtttt tagtatcatt tttatattct tattatacaa
    22021 attactatgc taactatgaa gtaatctatg gaagtatagc tgaaataatt gtacttatga
    22081 cctggatgta ttttagtagt tggtctatcg ttattggcta tgaggtaaat tcaagattgt
    22141 attttagaaa gataagacat gaaatgttaa agtaaagtat ttaaaataaa agagagtatt
    22201 tagtattata aatgtaattg aattaataaa gaaatattga catttaagtt aaaaagtcta
    22261 gaatttttct gggctttttt tatttttagt ataacttgac aaattttatt taatgggata
    22321 gttttaattt ataatcatct ttaaaaagat gactattgat aagtaagaga taaaattggt
    22381 aagtatcaaa aaatatacta aataaaaatt atagtcctaa tgagttaatt tctgtatttt
    22441 caaataatgc agtatttagt catgtgtttt tatat
//
LOCUS       DAELNY010000047        22303 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.047, whole genome shotgun sequence.
ACCESSION   DAELNY010000047 DAELNY010000000
VERSION     DAELNY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.047"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1254
                     /locus_tag="KPE55_001570"
     CDS             <1..1254
                     /locus_tag="KPE55_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0492659.1"
                     /translation="TIDKFVIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGN
                     EILRNLTKIIKRNIFEEEMFARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQ
                     EEKYKLVLSCGIYFILPEDRDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKE
                     KEMENTMFSSLENKEFIIYLQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFE
                     RNGFVINLDLYVLEEVCIYLRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKY
                     NIPAKYIELELTESIIFDNFDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMD
                     ILKLDQKFIMETYNSKRSKIIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMA
                     QGYLFGKPMPIEEFEHLIVSNLIRG"
     gene            1430..1798
                     /locus_tag="KPE55_001571"
     CDS             1430..1798
                     /locus_tag="KPE55_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0492660.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKITLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1800..2525
                     /locus_tag="KPE55_001572"
     CDS             1800..2525
                     /locus_tag="KPE55_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492661.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2519..3283
                     /locus_tag="KPE55_001573"
     CDS             2519..3283
                     /locus_tag="KPE55_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492662.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3505..4755
                     /locus_tag="KPE55_001574"
     CDS             3505..4755
                     /locus_tag="KPE55_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0492663.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYENSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVNGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            4946..5830
                     /locus_tag="KPE55_001575"
     CDS             4946..5830
                     /locus_tag="KPE55_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0492664.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6191..6808
                     /locus_tag="KPE55_001576"
     CDS             6191..6808
                     /locus_tag="KPE55_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG0492665.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6916..9447
                     /locus_tag="KPE55_001577"
     CDS             6916..9447
                     /locus_tag="KPE55_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0492666.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            9881..10582
                     /locus_tag="KPE55_001578"
     CDS             9881..10582
                     /locus_tag="KPE55_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0492667.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10632..11714
                     /locus_tag="KPE55_001579"
     CDS             10632..11714
                     /locus_tag="KPE55_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0492668.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            11975..13075
                     /gene="vanG"
                     /locus_tag="KPE55_001580"
     CDS             11975..13075
                     /gene="vanG"
                     /locus_tag="KPE55_001580"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0492669.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13072..13878
                     /locus_tag="KPE55_001581"
     CDS             13072..13878
                     /locus_tag="KPE55_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0492670.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            13896..16034
                     /gene="vanT"
                     /locus_tag="KPE55_001582"
     CDS             13896..16034
                     /gene="vanT"
                     /locus_tag="KPE55_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0492671.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            16348..16968
                     /locus_tag="KPE55_001583"
     CDS             16348..16968
                     /locus_tag="KPE55_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG0492672.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(17134..17670)
                     /locus_tag="KPE55_001584"
     CDS             complement(17134..17670)
                     /locus_tag="KPE55_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0492673.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(17783..18457)
                     /locus_tag="KPE55_001585"
     CDS             complement(17783..18457)
                     /locus_tag="KPE55_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG0492674.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLIATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            18660..20534
                     /gene="asnB"
                     /locus_tag="KPE55_001586"
     CDS             18660..20534
                     /gene="asnB"
                     /locus_tag="KPE55_001586"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0492675.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(20626..22062)
                     /locus_tag="KPE55_001587"
     CDS             complement(20626..22062)
                     /locus_tag="KPE55_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0492676.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         8517 a         2336 c         3774 g         7676 t
ORIGIN      
        1 actatagata aatttgtaat tgatgctaac aaaatactat gtaaaaatac tcaagtaaag
       61 tatgctttat tatatataga tattgataaa tttaaatata taaatgattt atttggatat
      121 gaagttggga atgaaatttt gcgtaattta acaaagatta ttaagaggaa tatatttgaa
      181 gaggaaatgt ttgcaagaat atctgcggat aattttatta taattatgaa atatatagaa
      241 gaagaagata taacaaagag gttaaaaact atatttgaag aattagactt gtttaataat
      301 aatcaagaag aaaaatataa gttagtttta agttgtggaa tctattttat acttccagaa
      361 gatagagata tcaactcaat catagatagg gctaatatac ctcataaaat ggcaaaggga
      421 ggacataaaa gttcatatgc attttatgat aataagattc atgaccaaga gataaaagaa
      481 aaagagatgg aaaatacaat gttttcaagt ctagaaaata aagaatttat tatatactta
      541 caacctaaga tagaacttaa tacaggagag atacaaggtt cagaagcact tgttaggtgg
      601 aaacgtcctg ataaagggct aatacctcca aatgaattta tcccattttt tgaaagaaac
      661 ggttttgtta taaatttaga tttatatgtg cttgaagaag tatgtatata ccttagaaaa
      721 tggattgatg caggaataaa tccagttact gtttcagtta atgtttcaag gatacattta
      781 tattgcaata attttataga aacatacaaa aatataattg ataaatacaa tatacctgca
      841 aagtatatag aacttgaatt aacggagagt atcatatttg ataattttga tattttaatt
      901 gatataatga ataatttaaa aaaaatagga tttttaatat ctatggatga ttttggttca
      961 gggtactcat cattaaatat gttaaaagaa attcctatgg atatcttaaa acttgaccaa
     1021 aaatttatta tggaaactta taatagtaaa agaagcaaaa taattgttac taaagtaata
     1081 gaaatggcaa aagagttggg tatgaaagtc atatcagaag gagttgagac agaagaacag
     1141 tttaaacttt taaaagaagt aaagtgtgat atggcacaag gatatttatt tggaaagcca
     1201 atgccaattg aagaatttga acatttaatt gtatccaact tgataagagg gtaattaatc
     1261 tgtaatataa agaatttaat aaattgtgtt aaaaatggac tctaatgtag tgttcatttt
     1321 ttttgtaaaa ttttttatac ttttactaaa aagtaattga catatacttt gtattattat
     1381 attattgtat taacaactta atacaataat ataataagga ggcatgaaca tggaatggga
     1441 acttgataat aacaaaccta tatatataca attagtagaa catttaaagt taaaaataat
     1501 ttcaggggaa attaaaatag gatctaaatt agaaacagtg agagcactag cagaagatgc
     1561 ggaagttaat ccaaatacaa tgcaaaaggc tctaacagaa cttgaaagac aaggtttagt
     1621 ttatagtcag agaactaaag gaagatttgt aacagatgat aaggaaaaaa taaaagctat
     1681 gaaagaggaa atagctaatg tggagataaa tacattaaaa ataaccttag aaaagcttgg
     1741 atatgataga gatgaaatgc taaaacttat tacagaaaat ctgaaaggag aattataaaa
     1801 tggataaaaa tataagcaat gtttctcaag atggaatagt tgaatttaaa ggtgtaaaca
     1861 agagttatgg aactaagaat gttttaaaaa atattgattt aaatatacca aagggaaaaa
     1921 tagtcgggct gttaggacct aatggaagtg gcaaaagtac aatgattaag ctaatgaatg
     1981 gattattaca gcctgacaat ggtgaaatta tgataaatgg aatgaaacca tccatagata
     2041 ctaaaaaaat agtttcatat ttaccagaaa gaacttattt aaatgattgg atgaaggtat
     2101 cagatttact taagtttttc tatgattttt atagtgattt tgatgttaga agagcaaatg
     2161 agatgattaa aagcttagat attgatgtaa atgaaaagct aaagacaatg tctaaaggaa
     2221 ctaaagaaaa agttcaactt attttagtaa tgtcaagaaa tgcgagcata tatatattgg
     2281 atgaacctat aggtggtgtt gacccagctg caaggtcata tatacttaag actatattaa
     2341 aaaattattc tgaagatagt actttactga ttgctaccca cttaataagt gaaatagaaa
     2401 acatatgtga tgagataata ttcatatcaa aaggagaaat agtgttacaa ggagacgttg
     2461 aagcaataag agaagaaaaa ggaaaatcta tagatgcatt atttagggag gaatttaaat
     2521 gttaggtaaa ttattaaaat atgagttaaa agcaagtgga agaatattta taccgttata
     2581 tatagccata ttgatagtag ctgtatttaa tggaatattt atgaatacaa atatacttca
     2641 ggtacaggga ataggaatac tagtattaac aagtcttttt atggcattag gagttttaac
     2701 tattgtagtt acaattcaaa ggtttagaaa aaatcttttg ggggatgaag gttatttaat
     2761 gtttactctt cctgttagca ctagttcact tatattatct aaatgtataa cagcacttat
     2821 atacgctgta cttagtttta tagttgcagt attcactttt ggagttctta tgctttttgg
     2881 tacttctgga attttactac ctgaaatttt agatttgttt aacactagtt ttaaatggat
     2941 ttctgaaaac tttttagata ttttattatt agtagtagta atgtttattt catattcatc
     3001 atttattttg ttattatata catcaatctc aatgggacaa ttaccaaaat tcaataaaca
     3061 tagaaacata gtggcatttg cttcttttat agctataaat atagtaatat caattgttgg
     3121 agatgcagtt ggaagcatct taccaaatga agatacaaat atggtatatc atctttatca
     3181 gagtcctagt tttatgcttg caatacttgg tagtcttgta gttgcaatag cgttattttt
     3241 cgctacaaaa tttatattag ataaaaaatt aaatttagaa taaatgtttt tatatggagc
     3301 catcttttga gtaatgttaa aagatggctc ttattattta tgtagttaat tcatgtatat
     3361 aatatgaata gtgttttatt tctagttaaa tatatacata tgttgtataa tgatattgcg
     3421 tgtaaaaatt tgtaagaata actatacact ttatataagt tatatcaaga aaatttaaaa
     3481 atatatggat ttgagggaga agatatgaaa agttatacaa aggctaagtg gagtgtttgg
     3541 ggtataatta cattttcatt tgttttagta ttatttttaa ggatgtcaac tgctgttgtt
     3601 tcagacaatt tagcaaatga gttaggtttt aattctatac aaatttcaaa tatagcatcc
     3661 ttttgcctat atgcatatgc atttatgcaa atacctgcag gaattttgat tgataaatat
     3721 ggagcaagaa aaattagcag tcttggaata ataatggcaa gtttagggtc aattttattt
     3781 ggtttgattc aaagtataga attagcatat atttctcgtg taattgtagg ggcaggaaca
     3841 tcagtaatat tactttgtat tttaaaaatt caagggagat ggtttaataa gtcagaattt
     3901 gcttctgcaa cagctaaatt ttcatttgta ggaaatttag gtggagttct tgctactttt
     3961 ccacttgtgt ttttgtctga acttgttggt tggagaaatt catttttatt aatcggtata
     4021 attggtgttg taattggatg ttttatgtat attattgtta gagatacacc aaaagagtat
     4081 ggattcaatg tagatactga accctatgaa aattcagaga aggttaatat tgttgatggt
     4141 attaagtctg ttataaagaa caaatcaaca tggtataatt caatgataat gttctctttt
     4201 gtgggtctta caagtgcatt tataagcttg tggggagtaa gatacataat ggacgtttat
     4261 ggtgtgagta agagtttttc tgcgtttata gtatcctttt ttacatatgg atttatattt
     4321 ggttcaataa ttatggattt tgtatttgct aaaataaggt ctagcaaatt taatataata
     4381 aaatttgggg caatgataga tttgtttata tggattgtaa ttgtagttgt ataccaggta
     4441 aaaccaccaa ttatagtttt accaatatca ttctttatta tgggatgtat agttatgtca
     4501 catctacagg tttttaatga tgccaagtat aaaaataaag aaatttattc tggactggca
     4561 actagtgtta ttaatacttt tgaatttata ggaagtggaa ttattaattt gattatagct
     4621 atctctttac aagtaaattc atataatata gttaatggat ataaaaaagg ttttgtagta
     4681 tttatagtac taagtattat aactatagta tcatctcata taggggtaaa aaatgatgat
     4741 tttaaggcta tatagtgttt tattaatagt ttaatttagt attatatttt gcatatatat
     4801 aacaaatagt ttgcattttc ctaagatgta taattggaat ataaatcttg tttactatgt
     4861 acaaatggta ttgtattata ttaaatagaa aataagaact ttaaactata atattggttc
     4921 taaaatataa ggtaaggagt tttttatgga tacgacgaaa atagatgata taagcaagtt
     4981 agatttaaat aatttagaca ttaataagat ggatatggga ggtataatgg aaagtctgct
     5041 agaatgggct actacaagcg gagtaaaact tataataggt atacttatac tttctattgg
     5101 atttaaaata ataaaaaaat ttgtaaacca tgttatgtta gtacttagta aaagagatat
     5161 tgatttaact ttaagaaggt ttttgaagtc tcttcttttg agtgtactta aagttgccgt
     5221 tataatcatt gttctggaat attggggaat gagtttgtcc agttttgctg ctgtgatagc
     5281 atcagcaggg gtagcaatag gtttggcttt acaaggtagt ctatcaaatt ttgctggagg
     5341 atttattata ctattgatta gaccttttaa agtaggagat tacgtagaag cagcaggcca
     5401 tggaggaacg gttgaacaaa taggactttt ttatacacaa ttggttactc cagataataa
     5461 acagatactt atacctaatg gttctgtatc aaatgatagt ctaataaact attcagcaaa
     5521 gaatactaga cgagttgatt tgacttttag tgtagggtat gaagatgata tactacatgt
     5581 tagaagagta cttaaagata ttgttaataa tcataaatta ataataaatg aaccagaacc
     5641 atttatagga gtagtagaac atggagataa tgctattaaa tttgcaacta gagtttggtg
     5701 caagacagaa gattattgga caatttatct tgatttacta gaagaggtta aagttaggtt
     5761 tgacgaagaa ggaattacga ttccatatcc taaaatggat ttaacagtaa aagaattaaa
     5821 caaaatttaa ttttttaata tagtgtttaa atttaagaat atcatataga tatacttata
     5881 aggtttgtaa aaaattaata agtactgtta ttttgtaagt aaaaatataa aaaatgggcg
     5941 tgtctcaaaa tgaacttttt agttcatgag gcactctttt tgtacgaaat aaaatatatt
     6001 tttattattt caacaatgaa aaagatgctt gtttctattt tgagacagta taatttttat
     6061 gtaactttaa ttttagtaaa tataaagttt taaaattgat aaaataggag aaatttttaa
     6121 tataaaaggg taaataaagg gttcagaggg cataatatag aataaattaa tatatttaag
     6181 gaggattttt atgtgtaaaa aaacgtattt attagtggta agtatactta tagcaggttt
     6241 attaactgca tgtagtggga gtccaactac ggattcagga caagcacaag ataatacaaa
     6301 aaaggaggca aatgcatcaa ataacgcttt aaaagatgaa aaaaataatg aaaatttaat
     6361 ggaacaagat tttaaagtgc catacacaga tgcaataaac atatttaaag ataaatataa
     6421 ggatgctgat atagtagatt taagtcttga gagagattta aataaatttg tttatactgt
     6481 ggaaggtgta gatgataata atgaatataa gatgaaaatt gatgctaata caaaagatgt
     6541 attagaagat aaaacagaaa agttggattc agaagattta aatggagttg ctagaaaaga
     6601 aaaattagat ttaaatgaca taatgacacc tcaacaagct atggaaattg cattaaaaga
     6661 acaaaatgga atagttaaag aatggagttt agacaaagat ttagatgtta ctttttataa
     6721 aataagaata gataaagata aaaatgaata tgacataaaa gtagattcta aaaagggaac
     6781 tgtattgaag gtagaaaaag aagattagaa gatttaaact atctttaaaa taatttcttt
     6841 tttgaataaa ttttgtttaa aatataacaa gcagggtata aagagactaa ataactattt
     6901 taggaggagt acggtatgtc attgaaatca ctaaaaataa aagaaaattt atattgggta
     6961 ggttcacttg accctgattt aagagtattt gacataatta tgtatacacc ttatgggact
     7021 acttataatt catatgtatt aaaaggaaca gaaaaaacag ttttatttga gacagttaaa
     7081 gataagcatt ttgacaatta tattgaaaga ttaaatgatt taaatataga ttttgaaaaa
     7141 atagactata ttgttgtaag tcatactgaa ccagaccatg caggaagtgt tgaaaagctt
     7201 ttggatttag ctaaaaatgc aaaagtagta gcttcagaaa cagcaattaa atatcttaag
     7261 gaaatcgtaa acaaagactt tgaatatgtt gcagttacag atggagatac attatcaatt
     7321 ggggacaaaa ctttagaatt tttctctgta cctatgcttc attggccaga taccatatac
     7381 acatatataa aagaggataa aactcttgta acatgtgatt catttggtag tcactatagt
     7441 aatgataaga tagtaaatac acttgatgaa aatgaagaaa aggattactt ggatgcttta
     7501 agatactatt atgattgtat aatgggtcca tttaaaccat ctatggtaac tgctatagaa
     7561 aagataaaag atttagatat agatactgta tgtccaggtc atggtccagt attaactgaa
     7621 aatcctagaa aaattataga cctttattac aactggagtg taaatgaaca aataaagtta
     7681 gaaaaagaag ttacaatttg ttatgtttct gcacatggat atacaaagat tatggcagaa
     7741 gctataaaag catatattga aaaaaatagc aactataaag taaatttatt tgatgtaata
     7801 gagcataagc aagaagatat tttggcaaaa atagcagtat cacaaggtgt attatttgga
     7861 acaccaacaa tattaggaga tgcgttaaag cctatatggg atatattaat atcgttaaac
     7921 ccagttcttc atggtggaaa agttgcttca gtatttggtt catatggatg gagtggagaa
     7981 ggtatagaaa atgctatgga gagaataagt cagcttagaa tgacagcagt aaaacctttt
     8041 gctgttaact ttaaaccatc aaatgaagag attgataaat tacattctta tacaggtaaa
     8101 ttcctagata agttaaattc tacttttgga agtaaaaaga aaactaagaa atttaaatgt
     8161 gtaatttgta atgaagtttt tgaaggtgat agtgctccta gtgtatgccc tgtatgtgga
     8221 gctaaggaag accaatttat agaagttgaa gaagatgaag ttacttttag aaaagatact
     8281 gatgaatatt tcgtaatagt aggtaatgga gcagctggtt tttatgcagc agatgctata
     8341 agaaaaagaa ataaaacttg taagataact atgatttcta atgaagatga gttgacttac
     8401 tatagacctg cactttctga tggtataaat gaagaacttg gttcagattt ctacatggaa
     8461 gataaagatt ggtatgataa aaataatata gtagtaatat taggaacgaa tgtagataaa
     8521 ttagatgaag tgaataaaac tattatagtg aatgatggag ctattaaatt tgataagctt
     8581 gtaatagcta caggaagtag aaactttata ccaccaatta agggccatga tttagaaaat
     8641 gtgtttactc ttagaaatat aaaagattta tatagtgtaa aagaagcact agaaaaatct
     8701 aagaaagttg tagtgattgg tggcggttta ttgggacttg aagctgcatg ggaatttaga
     8761 ctaaaaggat tagaagtagt agtagttgaa gccatggata gcatactatc aaaacaactt
     8821 gataaagaag gtagtaaaat attagaacaa tgtgtaagag atacaggaat agatgtaaga
     8881 ttgggtgtag cagttgatgg tattgaaggt gatggaaaag ctcaaaaagt tgtatttaaa
     8941 gatggagata gtgtcgactg tgatatggta gtattttcaa ttggtgtaag agcaaacact
     9001 caaatggttc aggatacatc tgtaaaaatt gatagaggta ttgttgtaga taagacatta
     9061 cagacaaatg ttaaagatat atatgcttgt ggagatgttg cacaagtggg aaatattagt
     9121 ttagcaatat ggccatcttc agtagaaatg ggaaaaatag caggggcaaa tgcaagtgga
     9181 gataatttga catttgaaag tgaagtatat ccagtttcat tagatgcaat gaatgtaaaa
     9241 gtatttagta ttggtaatat acaaaatttt gataaagaaa tttcatcaaa agatgaaggt
     9301 caaagaatat ataaaaaatt atttatgaaa gatggttcat tagttggagc tatacttata
     9361 aatgatttaa gctgtactgt taaattgata agattaatat ctgaaaaagg tgattttgag
     9421 gatattatga aggctgatat actataattt aaatacatta aaataaaaag gagttgtctt
     9481 aaaattgatt tttttctaat caaattttaa attcaactcc ttttttgaat ttttatataa
     9541 aataacatgg aaattttagt aatgtctgtg gttacataca taggtttaat tgttttttat
     9601 atggattgtc tctggtataa taaattgaat tgtgtttgac tgatagatat ttttattgaa
     9661 agttaatacc tgttatgcga taataaggac gtgttagggg agtttttata ataacaatta
     9721 atctcagttt ttgtgttcca atagtagcat caacacattc tgatgtagta gttcttttct
     9781 tataaaaatt agttacaaat atgcttgtaa ttatttgata tgtaaattta ccaatattat
     9841 aaatgatgac ctcgataata aggagagtga aaaataatct atgaacgaaa atattttgat
     9901 tgtggatgat gaaaaagaaa tagcagattt aattgaagta tatttaaaaa atgaaggttt
     9961 tacagtatac aagttttata caggtaagga agctctagaa tgtattgaat ctaaaaattt
    10021 ggatatggct attttagata ttatgcttcc tgatattgat ggatttcata tttgccagaa
    10081 aattcgagaa aaatactatt atcctatcat tatgttaaca gctaaaattg aagatataga
    10141 caagataatg ggtttaacca taggtgctga tgattatata acaaagccat ttaacccttt
    10201 ggaagttgtt gcaagagtaa agacacaatt aagaagatat atgcgatata ataattctta
    10261 tgaacaacag tcgataattg ttaatgagta tgatattaaa ggattgatta tcaataaaga
    10321 aacacataaa tgtagtttat ttgggaaaga agtagcatta acaccgattg aattttctat
    10381 tctttggtat ttatgtgaac atcaaggaaa agtggttcct tctgaagaat tgtttgaagc
    10441 tgtatgggga gaaaaatatc ttgataataa taacacagtt atggctcata tagggcgatt
    10501 acgtgaaaaa attcatgaac cttctaaaaa cccgaaattt ataaaaactg tatggggagt
    10561 aggttataca attgaaaagt aaaaatatta aagtcaatcc tgactttact caagtgagaa
    10621 ataaaatttt tatgagaaca gctttaatgg catttatagc cattattttc atatggattt
    10681 tatattcttt tatattgtat ggtaagtttt ctatctggat agtatcattc tttgaaaatg
    10741 cttttcgtat ggaatataaa gctgcattga atttatatca gcagattttc cgtaatagca
    10801 tggagctctt catgatacta gctatggtga ttgtgttttt tataatattc cgtgtttatt
    10861 taaattggtt tacaaaatat tttagtgaaa taaatcaagg tattgactct cttatcaaag
    10921 aagatgttgg agaagtggct ttatctccag agttattggc aatagaaaag aaaataaact
    10981 caattaaaca tatacttgaa caacgtaaat ttgagacaca aatggcagaa caacgtaaga
    11041 atgaacttat tgtttatctt gcacatgatt taaaaacacc acttacatca gttatagggt
    11101 atctgacact acttcgagat gaaagtcaga tttctgaaga actgcgaaaa aaatatttat
    11161 ctatttcgtt ggataaggca gagcgtcttg aagatttaat taatgaattc tttgaaatta
    11221 caaggtttaa tttatcaaat atatcacttg aatacagtac agtaagctta actcgtatgc
    11281 ttgaacaact aacatatgag tttaaaccta ctttgatgag taagaattta aaatatacac
    11341 tgaatgttac accaaatatg atgataaggt gtgatgttaa taaaatgcaa cgtgtatttg
    11401 ataacttatt acgaaatgct gtttattata gttttgaaga tactactatt gaaataacag
    11461 ctgtacagga ggatgattat ataaagctta aatttatgaa tcatagcaat acgattccag
    11521 aggaaaaact tgagcgtatt tttgaacaat tttacaggtt ggatacatct cgtaattcaa
    11581 acagtggtag agctgggttg ggacttgcta tagctaaaga aatagtggag cttcataacg
    11641 ggacaataac agctcatagc gagaacaata taattacctt taaggttatt attccgattg
    11701 taggaaattt gtaagaattt aagtataaaa aaataggaaa ttctttagga taaataaata
    11761 aagagcactc ttgttattga tataattact gtatcaaaac aagagtgctt ttattttgat
    11821 acagtaatta tatcaataga aaaatgtgaa aatatattat gtggaacaga tgcaattgta
    11881 agtgaaggtt gattggattt gaaatagaaa aagtatttta acatgtttct gtagattgta
    11941 taaaaataaa taaatcaaca aaggagaatc aaagatgaat aataaaaaaa ttgcagttat
    12001 atttggtgga aactcaacag aatatgatgt atcattacaa tcagcttttg cagtacttga
    12061 gaatttgaat acggaaaaat atgatattat accaattgga attacaaggg agggtaattg
    12121 gtatcattat attggagatt ataataatat ccagaataac acttggtttg aaagggatac
    12181 tgatataatt ccagttattg tttcgcagaa ccgtgtcaaa aatggtatta ttgaattttt
    12241 aattcctgat gctgtttctc agaatcatat tccaaatagt gctattgaat catgtgaaaa
    12301 tgatattaaa tacactaaac ttgattttgt gtttcctgta ctgcatggaa aaaatggaga
    12361 agatggaaca gtgcaggggt tatttgaatt ggcaggtata caggttattg gatgtggtac
    12421 tctttcttca gcattatgta tggacaaaga caaatcacat aaacttgttc attcagaagg
    12481 tattaaagta ccaaaatcaa tattactaaa atactcagat aaatttaatg tggctgaact
    12541 gtcaaaagac ttaacttatc cattatttat aaaacctgtg cgtgcaggtt catcatttgg
    12601 tattagtaaa atttataaaa aagaagagtt acaagtagca gtagacttgg cttttgaaca
    12661 tgataatgaa gtaataattg aggaaaatat tgatggtttt gaggtagggt gtgcagtttt
    12721 aggaaacgat gacttacttg ttggaagagt agatgaaata gaattgtcaa aaggattttt
    12781 tgattataca gaaaaatata cattgaaaag ttcaaaaatt catatgccag ctagaattga
    12841 tttggattgt gagaaaagaa tacaagagac agcaaaaatt atttatagaa ctcttggatg
    12901 ttctggattt gctagagtag atatgttttt aactccatct ggtgaaatta tttttaatga
    12961 agtcaataca attcctggct ttacttctca tagtcgttat ccaaatatga tgaaaggtat
    13021 tggactgtct tttgaagaaa tattggacaa gataattgag gtgtacataa aatgatgaat
    13081 aatattttat tagataaaaa taaagaagat aaaatgacaa atactatctt attgactaaa
    13141 gataaagttt atgatgggaa tctaattcta gtaaatgctt ttttaccagt taaaacatca
    13201 gaagatatag atttaattcc tgtagataca aggtttccaa gtattcttat gaaacgtgaa
    13261 gctacaaaca ttttacaaaa tattttaaaa agtatatgtg gaataaatga aatagtacct
    13321 gtaagtggat atcgtactgc agaggaacaa caagatatat attcaagttc tctgcgtgac
    13381 aatggaaaag actttactaa aaagtttgta gcacttccaa atcacagtga acatcagaca
    13441 gggcttgcta ttgatttagg gttaaagaaa gaagttatag attttatctg ccctgaattt
    13501 ccttataatg gtatttgtaa tgagtttcgt aaggcagcac tacattatgg ttttatagag
    13561 cgttatcagt gtaaaaaaga agaaataaca ggaatatctc aagaaccatg gcattttcgt
    13621 tatgtgggat atccgcattc tgagattatc aataaaatga atctcgctct tgaagaatat
    13681 atagaatata ttaagagatt ttcgtataca aattgtttaa aaatagaaga taaagaaaaa
    13741 attatagaaa tcttttatgt gcctatccat actaaagaag aagtgagaat ctctatacct
    13801 gaacaatgtg tatatcaaat atcgggtaac aataaggatg gttttattgt gacattttgg
    13861 agaaatagaa aggactaaga aatggaggaa caagaatgat taggaataaa tcttatgcag
    13921 ggcttgatta ttttaggatt atagctgcat tacttataat tgcaattcat acatctccat
    13981 tattatcata tagtaaaatg gctgactttg tgttaactag tataattgct cgtattgctg
    14041 tgccattctt ttttatgcta tcaggctttt ttctaatttc aacatataat tataattgta
    14101 ataaactgaa atcttttcta aaaaatgtat tatttattta cgggatagct attgtaattt
    14161 atattccctt aaatatttac actggatatt tttccatgaa taatttagtg cccaatatta
    14221 ttaaggacat tgtatttgat gggactttat atcatttatg gtatctaccc gcatcaatta
    14281 ttggtgcaag tattgcatac tttcttgtaa aaaaactagg tttaaagtac tcactagtaa
    14341 tcactattgt tatttatgta attggtatgt ttggtgatag ttattatggt tttttagaaa
    14401 aaataccact attaaagaat ttatatgggt atatttttga agtatcagat tatacacgaa
    14461 atggtatatt ttttgcacct gtatttttca tacttggtgg aataatatct aataaatcta
    14521 attatatgtc aactaaaaat agcttgattg gatttcttat atcattctta cttatgttat
    14581 gtgagggaat gtttttacat aaactagggg tacagcgtca tgatagcatg tatattatgt
    14641 tattgccatg tatgtatttc ttatttacag ccttaacatt tttgaaaggt tctcgagcta
    14701 ggttattgcg aacatcatca ttaattattt atattataca tcctatgatg attgttgtta
    14761 ttcgtatgtt ttctaaaata ttcggaatac aaactgtctt ggttgacaat agccttattc
    14821 attatatagt ggtaagtata gcatctttta tagcttctat aattattata ttgattctta
    14881 aacatctaaa aaaagacttg aaaggagaac ataaggtaaa taccaataga gcttggattg
    14941 aaattgattt aaataatttg gaacataatg taaatatgat acaaaaagct atgccagaag
    15001 attgtgaatt gatggcagtt gtaaaagcta atgcctatgg tcatagtgct tttgaagtag
    15061 cgacttgtgc aaaccaaata ggtgtaaaag catttgcagt agcaacaata gatgagggga
    15121 ttgatttacg tcgatatgga atactaggag aaatattgat tcttggatat actaacccaa
    15181 taagggcaaa ggaacttcat aaatacaatc ttacacagag tgttattgac cttaatcatg
    15241 ctatctcttt aaacaaacag aaatacaaca taaaatcaca cattaaaatt gatacaggta
    15301 tgcacagact gggttttgat gttaatgatg tgaggtcaat tttaaaggca tttaaattaa
    15361 aatacttaga tatatgtggc atatttacac acttgtgtgt ttcagatagt ctgaaagatg
    15421 aagatgtaga ctttacaaat aaacaaattg aatgttttta taagttaatt gattttctgt
    15481 taaaaaaagg tataaaaatt cctaaagtac atattcaaag tagttatggt ttttttaatt
    15541 atcccaatct taagtgtaat tatgtaagaa ttggaattgc attatacggg atattaagtt
    15601 ctccaaatga tacgactaaa ttgcaacttg atttacgacc tgttttatcc ttaaaatcac
    15661 aagtaattct tattagaaaa atacaaaaag atgatagttt tggatatgga agagtcttta
    15721 ttgcaagcag agatagcgta attgctatat tatctattgg ctatgccgat ggtataccta
    15781 gaaatttatc ctgtggaaaa agttacgtaa taataaatga ttgtagagca gcaatagtgg
    15841 gtcgaatttg catggaccaa cttgctgttg atattacaga tatacctaat gtagaagttg
    15901 gtaatacggc aataataata ggaagagata atttagctga attatctgct tctgaagttg
    15961 caaataactc atgtagtata tcaaatgaat tattgagcag agtaggaaga aggttaaatg
    16021 ttataaagaa gtaactcaat taaatagtaa tatttgttat actgtgtaat ttattttact
    16081 ctgactagac atttttagta atattatgtc taatatatat tttttataac ttacatgaca
    16141 atttaagtta taaaaaggtg atgtttcaga tttttatttt gaaacatcac cttttttgtt
    16201 ataatatata ataaaataat tataccttaa ctgtaaactt atatagatta tttacttttt
    16261 aatattagat tcattttttg agattggtat ataataaaca tatagagtat ataataatag
    16321 ttaaagttaa aaaaggggaa aagcaatatg cagtatatac aagaactact tgatttgact
    16381 caaaattatt gggtgttagc cacaataatc ggcttattaa gtgcatttat agaaagcttt
    16441 ataccagcct taccacttgt tgctatagtt acagcaaatg ctgcaataca aggattattt
    16501 attggatgtt tgttatcatg gattggctca ggacttggga caacttcatt attcttatta
    16561 ataagcagat ttaatgatag taagcttttt aataagttga gaaatagtaa aactgaaaaa
    16621 gcaataagtt ggatggataa acagggattt aagttattat ttatagcgta tgcctgtcca
    16681 tttatgcctg gttgtttagt tactatcgca tcagcttttg gtaaaaaaga tttaaaagat
    16741 tttgtgccag caatgttggc aggaaaattt gtaatgttta tagttataag ttacgtggca
    16801 agtgatatag aaggttttat aacaagtcct ttaaagatag catcatttat attattggtg
    16861 tttttgtcat ggaagattgg aaatagagta aataaaaatc ttgaaaatca taattatgat
    16921 ttccatcata aaaaacatca caatgataaa gatgataaaa tgatttagct tgtacgaaat
    16981 aaaacgtatc ttaaaatgat ttagtactaa attattttaa gatacgtttt attttttgta
    17041 ttaaggtaca aaatttttag ttcttttgta aactaaaata tataaaactt atttatactt
    17101 agttcaaaat attgttattt attttttcta caactataat aaaatataaa attgatatcc
    17161 aatcaaaaaa ataactatga aatttaatag cttagatatg aatttttctg tttttgagtt
    17221 agtttttata ggtagtaaag aaatgtttat ggtaatggga taaaacaatt caataccttc
    17281 tttagtaata aggtcaagac acaagtgaga tagatatcct attataaacc cactggacaa
    17341 acatgagaaa actatattat tgttagtgaa gattgtcaga gatttaaaaa aactataaat
    17401 aagtaataag gcaattagac tgtgagtaaa ccctctatgt ctaaacctac ttccaaataa
    17461 tttgtatatt aacataaatt ttttacttat gtaagaacct ctcatatcta tatctggaaa
    17521 taaagaacct aaatatgcaa aataaatata aattataatt aatactattt tatatgcaat
    17581 atcgtattta actaggtaca gattgtttat aaaggggagt atcaaaagtg cgaatatata
    17641 acctcccctt tcatgtgttt cttttatcat ataaaaatcc tcctgaatcg tcactataaa
    17701 cattatattg gtaaatttag actatctttt attatattat atagcaaaca taagtttttt
    17761 tcaaaaggat tttaatattt aattaatctt gagattttaa atttttgtaa ttttctaaag
    17821 caccattcca atttactaca ttaaaccagt tgtcaatgta ttcatttctt ctattttgat
    17881 attttaaata gtaagcatgc tcccaaacat caagtcctat tataggagtt aggtttttac
    17941 ttacagggct atcctgattt ggagtagtca taatagataa tttgccatct ttagtagcta
    18001 taagccaagc ccaaccagaa ccaaagacat ctaaagcaga tttttggaac tcttgcttaa
    18061 atttttcaaa agaaccaaag tctctatcaa tagcttcttt taaagattca gaaggtatgg
    18121 ttttttctgg tgtcattata tcaaaaaaga atttatgatt ataagctcca cctgcattat
    18181 ttcttacagt tgtagcaata tctttaggta aagaatctaa attttgcaat aattcacata
    18241 aagaataatt ataaagctca ggatattttt caagagcagc atttaattta tcaacataag
    18301 cttgataatg cttatcatga tgcagtttca ttgtttcttt atctatataa ggttcaagtg
    18361 catcatatgc ataaggtaat ggtttaacct taaatttgtt attttcaggt gtaaaagcaa
    18421 atgaagttat gcactgtgag attataaata aagacataat aactggtatt aatatttttt
    18481 tcttcataaa aatcacctcc taatatagta tttctagaaa taaaaaaaat atgaaatatt
    18541 aacagataat ttataagaat ttaatagcaa tttcatttta aattgaataa gttttaaagc
    18601 caaagacaaa actatgaaat atagaataag actaaaggac agaaaggaat tttgattata
    18661 tggacggcaa tatttcaatt tattataaag gtgatatatc taaaaaagaa gatattaaat
    18721 ttaaaataaa agaatctgta aataatacaa gactttctag tagaatagag tataaggatg
    18781 aggaaaaaac ttttgatttt gaaagattta atgtaattga tgctatttat ggaaataaac
    18841 catttgaaaa agatggaaaa gttattgttt ttaatggaaa tatatataac tatcatgaaa
    18901 taaaagaaga attaattaaa aaaggacaca attttactac aaatgatgat attgaagtct
    18961 tattagcatc ttatatagaa tttggtaaga attgtgtaca taagattaaa ggaatgttca
    19021 actttataat ctatgataga gaaaatgaaa gtatatttgg agcaagagat ttatttggga
    19081 taaaaccact ttattatata aataaggaaa atgcaataat tttttcctca gagtataagt
    19141 ttttacttga atatatgaaa aatttaaata taaatgaaag aagcttacaa agttattttt
    19201 catttcaata tgtacttcct gaagatacta tgatacaagg aataaggctg ataccagcag
    19261 gtcactattt tagggtagaa aatggtatac tatctttaaa gagatataat aaacttgagt
    19321 ttagaagtag tacaaaattt ttttatacta aaaatcactt aggcaataga gatgtgaatg
    19381 aggatgatat aagaaatata gtagtagatt caatagtaac tcatatggaa gaggagaaag
    19441 aaattggaac atttttgtct ggtggaatag attcatctat tataactact gtggcttcac
    19501 aaattaatcc aaatataaaa tctttttctg caggctttag tgtcaaagga tatagtgaat
    19561 tagaagtagc aaagaaaaca gctgataagt taggtataga aaatatccag attaatataa
    19621 cacaagatga atatataaaa tcattaccga atgtaattta ttctttggat gaccctatag
    19681 cagacccttc agaagtaggt ctttatttct taattaaaga ggcaggtaaa catgttaaag
    19741 tagcattgtc tggagaagga gcagatgaat tatttggagg atataatata tataaagagt
    19801 atagtactat gaaatcagtt gtcaattcac ctacttatat taaaagtata ttaggaagag
    19861 tttcagaact tatgccaaac ataaaaggaa gaaattatct ttatagagct acaacaccat
    19921 tagaaaaaag atatatagga aatgcaaaag tttttgaaaa cagcgaagta aaaagattct
    19981 tttttaagta taaggaaaag aatatatatg aatacctgtt gtctaatttg tatagagatg
    20041 cacaaaaaaa taattacgat tatataagta aaatgcaaca tatagatgtg aatacatggc
    20101 ttcaaggcga tatacttcaa aaaattagca aactttctac agctgagcag gttgaactta
    20161 gagtaccatt tttatataaa gatgtatttg atgtcgcaaa aaatttaaga atggagcaaa
    20221 aaataaataa gaataataca aaagttttac ttagagaggc gtttagagaa atagtcccag
    20281 aacatgttgt tcaaaggaaa aaattaggat ttccaactcc aataagagtt tggttaaaag
    20341 attctctagg ggatattgta aaggaaacta tttataattc aaatgtagat gaatttatag
    20401 ataaaaaata tgcaataaaa cttttagata cgcatcttaa aggtcataga gataattcga
    20461 gaaaaatatg gactatattt acattttgtc tatggcatca attatttatt gagcataaaa
    20521 atgtcgagta ttaatacttt tttcaatatt aatcagaggg caaaagcatg caatgttttc
    20581 accctcttga ttattatgga ataggataag tagaattttg attgattagt ttactatctc
    20641 aataaaatta ttggcaaata ttttgacaag actactttca acagctaaat ttaagttttg
    20701 tccatcatca tatccagcgg aaatcaatcc tagtaagttt ccaaacatat ctaaaagagc
    20761 tcctcctgaa ctaccactag agattggagc tgtaaattgt atcatttgaa ccgtttcaaa
    20821 atcacgaaaa gctgaaataa ttccatctga aactgtatta aaaagaccta gagggcttcc
    20881 aattgcaact attttttgac ctctcacagg ttttttactg actttaacag gtattggctt
    20941 gcattttcta tcaaccttta tcacagctaa atcataatct gaatggtatt ttataatttg
    21001 gtaagatgta taaactttat catcattttc aaatctaact aaaaatgaat acccatcatt
    21061 gactacatga agattcgtaa gtatataacc ttcattattt attactactc cagaaccgct
    21121 cttaaacggt ttattttcgt tattgcacac aacaatcata acaacagaag ctgataactt
    21181 tgcaaggtct tcataagaaa gttctacttc ctcatcttgt acaatttttt tagatgttag
    21241 atttgtgtta ttaagtgtat ttttagagtt aggtttattc gtagtaggag aagtcttatt
    21301 ggtattttca ctgtaattta tatttgtaat atgcctttca aaaggtattt ctaatatatt
    21361 tggagtcttc ctagaaagtt caaatagagt agaaaatttt gaacattcag gatataaatc
    21421 aagaatatct ttatttggat ttatatataa aatatcacac ccaagttgtg ataaaaaata
    21481 taagaaaaaa agttcttgcc ttttaatatt tccaaagtat actatctttg gtgaaatagt
    21541 agtcttcacc ttgttataaa ataatttagg tagaaaatag tctatccaac acaaaagttt
    21601 aactgcaaag tttttgatga tagaggatgt acaatttggc atacttgtgg aaaataaatc
    21661 taaagtattt ttaaatgcaa tctttttagt ccaatttaag gtgtcatttt ctattttaca
    21721 tggaaagtcg atattatata aatcccaact gttaaactta gaactattat aaatattaaa
    21781 aattttaagc atcttatctg tttgttcttt gtcaaaattt atttttagat tgtttaatct
    21841 aatataggga attgtaggat tattaaaaaa ataagtatct atatttttaa ttttttctat
    21901 gtatttatca attgaatcat caacacctat atatcttgta aagtagactt ctttaatata
    21961 tgaagaactc ttatttttat aggtagtatc agatataaat tcaattagag gttcagtaga
    22021 cttttttatt gaaatcttta atggtttaat agtacaaatc atattttttc tcctataata
    22081 agatgatata atctattata aaacaactta aaacaatata gtacaataat tttgtagttt
    22141 caaaataaaa tatgtacttg tttatataaa tgatatataa ttacaataaa attgccgaaa
    22201 agccctctcc tttaggtgta gggatgaaag gtaaggtctt aatataaaaa aagttagaat
    22261 aatgcttgta atctaatacc gctactgata ccctatattt agg
//
LOCUS       DAELNY010000048        22059 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.048, whole genome shotgun sequence.
ACCESSION   DAELNY010000048 DAELNY010000000
VERSION     DAELNY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.048"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            693..2363
                     /locus_tag="KPE55_001588"
     CDS             693..2363
                     /locus_tag="KPE55_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG0492677.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2485..3219)
                     /gene="pflA"
                     /locus_tag="KPE55_001589"
     CDS             complement(2485..3219)
                     /gene="pflA"
                     /locus_tag="KPE55_001589"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG0492678.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3379..5610)
                     /gene="pflB"
                     /locus_tag="KPE55_001590"
     CDS             complement(3379..5610)
                     /gene="pflB"
                     /locus_tag="KPE55_001590"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG0492679.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5903..6964
                     /locus_tag="KPE55_001591"
     CDS             5903..6964
                     /locus_tag="KPE55_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG0492680.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7055..8668
                     /locus_tag="KPE55_001592"
     CDS             7055..8668
                     /locus_tag="KPE55_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0492681.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8943..10928
                     /locus_tag="KPE55_001593"
     CDS             8943..10928
                     /locus_tag="KPE55_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0492682.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            10933..11367
                     /locus_tag="KPE55_001594"
     CDS             10933..11367
                     /locus_tag="KPE55_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG0492683.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11396..11956
                     /gene="srlA"
                     /locus_tag="KPE55_001595"
     CDS             11396..11956
                     /gene="srlA"
                     /locus_tag="KPE55_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0492684.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12020..12388
                     /locus_tag="KPE55_001596"
     CDS             12020..12388
                     /locus_tag="KPE55_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG0492685.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12413..12979
                     /locus_tag="KPE55_001597"
     CDS             12413..12979
                     /locus_tag="KPE55_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0492686.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13020..13385
                     /locus_tag="KPE55_001598"
     CDS             13020..13385
                     /locus_tag="KPE55_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0492687.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13486..14259
                     /gene="srlD"
                     /locus_tag="KPE55_001599"
     CDS             13486..14259
                     /gene="srlD"
                     /locus_tag="KPE55_001599"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG0492688.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14607..16877
                     /locus_tag="KPE55_001600"
     CDS             14607..16877
                     /locus_tag="KPE55_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0492689.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDITSNKVNYVSDELDMTSNKVNYVLDELDITSNKIDYISNELYNL
                     SNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMF
                     IPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVE
                     KRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKN
                     LGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
                     VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFG
                     GQVTYNESMVEIIRQN"
     gene            16965..17300
                     /gene="spoIIAA"
                     /locus_tag="KPE55_001601"
     CDS             16965..17300
                     /gene="spoIIAA"
                     /locus_tag="KPE55_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG0492690.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17314..17742
                     /locus_tag="KPE55_001602"
     CDS             17314..17742
                     /locus_tag="KPE55_001602"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG0492691.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17765..18529
                     /gene="sigF"
                     /locus_tag="KPE55_001603"
     CDS             17765..18529
                     /gene="sigF"
                     /locus_tag="KPE55_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG0492692.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18809..19246
                     /gene="spoVAC"
                     /locus_tag="KPE55_001604"
     CDS             18809..19246
                     /gene="spoVAC"
                     /locus_tag="KPE55_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG0492693.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19400..20413
                     /gene="spoVAD"
                     /locus_tag="KPE55_001605"
     CDS             19400..20413
                     /gene="spoVAD"
                     /locus_tag="KPE55_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG0492694.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20427..20789
                     /gene="spoVAE"
                     /locus_tag="KPE55_001606"
     CDS             20427..20789
                     /gene="spoVAE"
                     /locus_tag="KPE55_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG0492695.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            20972..21988
                     /locus_tag="KPE55_001607"
     CDS             20972..21988
                     /locus_tag="KPE55_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0492696.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         8604 a         2479 c         3842 g         7134 t
ORIGIN      
        1 aaaatccaga aaaatatact taaattatgt aagtaaaaga ggggttgaat cagttgaaaa
       61 tgtaatatta gtaaatagta catcaatcct atataccctg taagcaactc tattttcatg
      121 tactaaaaat gtacctattt tattaactga aaagaataaa ttaaatgtaa gtacataaga
      181 agatagaaaa aattaaaaac aaaaaatatt tatataaata aatataaaaa atgatataat
      241 ccctattaaa aaatttatag atgatgaaga aatatcaaaa tcttatatta taggaagaga
      301 cacttctttg ccaaaatcta tagaaaagca ataccaaatc caaagagaat aggcgaagaa
      361 aaaaataaaa taaattcaag agtaatagaa aagttttata gaataaagaa ctgataactc
      421 tttatataac gaaaaataga attttaagag aaaatgattt aataaatgat ttaacatggt
      481 gttttagcag gtaaaaataa atcaccagta atgattatag aaaaattact ttatgaatct
      541 caaaaaactt agttaagagt aagagatttg ataagataac taaagttggg gaaatgtgtt
      601 tgatgagaca aaaaattagt aaaatagtat aatctaaata aggatatttt gttgattaaa
      661 ctacactaat agtaatattg tagggggaaa agatgagaga agaatcgaac tctttaagtt
      721 tagaccatat agaaattttc tttgaacttt tatctcaaag tacagaagat tatatatttt
      781 tttgggatat aaataggaat aaatttaaaa tttcttcagc aatttttgat gaatttaact
      841 taagtaaaga gatagaatct gatttggtca attgttggag taaaattgtt tatccggatg
      901 atgttcaaat atggaaagat gatatacaag aattactaca tggaaaaaaa ggtgagcaca
      961 atttagaata taggttgatt aataaatata aagaaatagt atggatttct tgtagaggta
     1021 aagtttatgt gtcggatgac ccaaaaacta tatttctagt cgggagaata aagaatattg
     1081 gtgaaaaaaa taaatttgat tctatcacag gaacgtggaa tagagaacaa tttgaacata
     1141 gaatgaatta tcttataaaa gagaaaattt ataaaaatgg tgctatgttt attatggata
     1201 ttgataattt taaaaatata aatgaaaaat atggacattc ttatggtgac aaagtattgc
     1261 gtgcaattgc tactgaagta ttagaatatc ttccaaaaga tgtaagattg tataggttag
     1321 atggagatga atttgcattt ttttacccta tgtgtactaa agaaactata gagaaaatat
     1381 atgaaaagat acagatgtat acaaatactc agcatgaaat tgaatcaaat aagtactatt
     1441 gcacagtgac tgcaggcgtt gctatgtatc cagaagatgg agataattat ttagatttat
     1501 ttaaacatgc tgatattgca ttggatatag caaaaattag tggtaaaaat agaattaagt
     1561 ttttttcaca agagctctat gaaaataaac taaaggtaat ttcaatgcaa caaaaactaa
     1621 gagagtgcgt tgaaaataat tttaatgact ttgagctgtt ttttcaaccc caggtaaatg
     1681 ctgttacaaa agaggtaata ggggcagagg tacttttaag atggcattcg agtacttatg
     1741 gagaagtatc tcctgttgaa tttataccaa tactagaaca gagtaattta attataccag
     1801 ttgggaagtg gattataaaa gaagctgtaa aacagtgtaa agaatggcat aaaataaatc
     1861 cagactttaa aatatctgta aatgtatcgt atattcagtt aaaggaagat ttttttagag
     1921 attttatagt agaatgttta gtagagtatc aattaagacc tgaattttta attttggaat
     1981 taacagagaa ttgttggata ccagatataa atttactaaa tgataaattt attagtttaa
     2041 agggtatagg agtatatatt gcaatagatg actttggtac aggatattca tctttaaatt
     2101 accttaaaga actgtctgtt aatataataa aaattgaaag aagttttgtt aagaatatta
     2161 catataatag ttatgaatat acttttcttg aatatattat taaattagct catattataa
     2221 atttaaaagt ttgtgttgag ggaatagagt cttatgaaga gtatgatata gtaaaaagct
     2281 taggggtaga tattattcag ggttttttat ttggaagacc tgtaagtgcc tctgaatttt
     2341 ataaatttca actatcaaag tgatgtatat aaaatgagat atatagtata taaaaatagg
     2401 gcaaagtgga agttttgaag tttattaaaa atttacactt taccctattt tgttatgtct
     2461 tctataaagg attaatatac tacactaact attatatact ttcactccaa atttagagat
     2521 tatttcacta gctttttgga catcttcctt agtggcatca ggaacatctt ttagttcata
     2581 atcgatacct aaactttccc atttgtgtat tcccatagaa tggtatggta gcaattcaaa
     2641 tctttctaca tttttcaatg tagctacaaa ttgacctagt ttttctaaat tatctatatc
     2701 atcagtaatg ccaggaacta aaacatgtct tatccataca ggaatatttc ttgattctaa
     2761 gtgtctagca agttttaatg ccttatccat accaacacca gtcaaatcaa tagatttttc
     2821 ctcaaccata tgttttaaat caagtaaaac caaatcagta ttatctaaaa ctgggtctat
     2881 agtttctata tctacaaatc cagatgtatc caagcaagtg tgtatatcat tttcttttgc
     2941 ttttttaaat aattcactta aaaattctgg ttgaagagtg gattcaccac cagaggcagt
     3001 tatacctcca ccagaaaact tcataaaaga agtatactta agagcatccg ttattatttc
     3061 atcaactgtg tattcttttc ctgatttaat atcccatgta tccctgttat gacagtattt
     3121 gcatctaaga ggacacccct gaaaaaataa tatatatctt attcctggtc catctacagt
     3181 accaaacgtt tctatagaat gtatctttcc cttaatcata gagttcacct cacaatatat
     3241 tttaataacc ttatcaatag ttataatatt tctctaaaaa aggccatctt ataccagaaa
     3301 taattcaaat ataagatagc cttttatttt tattttttac acagtagtaa tcttagttaa
     3361 agtataaaaa tttaaagact aagccatttt accgtggaat gttctgttta taacatctaa
     3421 ttgttgctct ttagttaatt ttatgaagtt aactgcatat ccagatactc ttatagttaa
     3481 ttgtggatat tcttctggat gttccatagc atcttctaaa gtagctctat caaatacatt
     3541 tacatttaag tgatgagcat tttgagcgaa gtatccatcc atcattgcag aaagattgtt
     3601 tattctttca gtcatatctt ttcctaaagc agcaggtact atagagaaag tattagatat
     3661 accatcttga gcatgttcat atggtaattt agcaactgaa gataatgaag ctaaagcacc
     3721 attagtatct ctaccatgca ttgggttagc accaggagca aatggagcac cagctcttct
     3781 accatctggt gtattaccag ttttcttacc atagacaaca tttgaagtta ttgtaagtat
     3841 agattgagta ggataagaat ttctataagt tttattcttt cttattttat tcataaagct
     3901 ttcaactaac tctacagcta tactatcaac tctatcatca ttatttccgt attttggata
     3961 atctccttct atttcaaaat caacagctat tccttgttca tttctaatag ttttaacctt
     4021 agcatgtttt atagcagata atgagtctgc acaaactgat aatccagcca taccacaagc
     4081 catagttcta aatatatctc tatcatgtaa tgccatttct aatgcttcat aagaatattt
     4141 atcatgcata taatgtatta catttaaagt atttacatat aatgttgcta accaatcagt
     4201 gaatggttca aatttactca taacttcatc ataatctaag tactcagaag ttataggttc
     4261 aaatcttggt ccaacttgaa caccagattt ctcatctaca ccaccattta tagcgtataa
     4321 taaagtttta gctaagttaa ctctagctcc aaagaactgc atttgtttac caattctcat
     4381 tgcagataca caacaagcta taccatagtc atctccccag tatgctctca ttaaatcatc
     4441 attttcatac tgtactgaac ttgtatctat agatacttta gaacagaaat ctttgaatcc
     4501 ttgaggtaat ttagttgacc atagaactgt taagtttggt tctggagaag gccctaatgt
     4561 atatagagta tttaacattc taaatgagtt tttagtaact aatgttctac catctattcc
     4621 catacctcct atacactcag ttacccaagt tgggtctcca gagaataagt tattgtaatc
     4681 aggagttctt aggaacttaa ccattcttaa tttcataaca aaatggtcca tatactcttg
     4741 taattcttct tctgttacga ttccagcttt taaatctctt tcaaagtata tatctaagaa
     4801 agtagatgtt ctacctaaag acattgcagc accattttgg tctttaacag cacctaaata
     4861 accaaagtat aaccattgta cagcttcttt tgagttagtt gctggttttg atatatcaaa
     4921 tccataagat tcagccattt ttttaagttc atttaaagct gatatttgct cagttatttc
     4981 ttctcttaat ctgataactt cctcatccat gcaagaaact tctaatgatt tcttttgctc
     5041 atttttatct tctattaaag catcaacacc atatagagca actcttctgt agtcaccaat
     5101 tattctacct ctaccataag catctggaag acctgttatt ataccagatt ttctagcagc
     5161 tctcatttct ggagtataaa catcaaaaac tccttggttg tgtgtttttc tgtatttagt
     5221 aaatatatct tttatttctg ggtctaattc atatccaaaa gcttcgcatg aattttcaac
     5281 catctttata ccaccatatg gcatgacagc tctttttaaa ggggcatccg tttgtaaccc
     5341 aactattgtt tctaattctt tatctaaata tcctggagca tatgcagcta ttccagaaac
     5401 agtttttgta tcaacatcta aagtaccacc attttctctt tcttttttaa ataggaccat
     5461 tgcttcgtcc cataattttt tagtattttc agtggcacca gctaagaaag agtcatttcc
     5521 ctcatatgga gaatagttta attgtataaa ctctctaaca tttatttctt tagtccatct
     5581 tcctgtttta aatccttgcc atgcattcat taagtatttc ctccttagat ttaaatgata
     5641 aataatacta ttaaaattaa tttttatata cttaatttat tttatatatt tttttaaaaa
     5701 tatattttct aatataataa tagcatatta ttcgtatcct gtatacataa aaaataaaaa
     5761 attgtcgctt taaagtataa aacagattat atgttaataa tgtggtgaaa ttatacccat
     5821 atataaataa aataaaacaa ataaatagct aattaaggag aaaatatttt ttattttttg
     5881 tttaggataa ataaatttta ttatggaaaa aagaaaaagc atacatcttc acaaactgaa
     5941 tatttattta atagggggag gtgatagatt gtttatcaca gtggtattat ttttagtagg
     6001 tttcctatta attactaaag gcgcagatat ctttataaat tgtactgtag agataggtaa
     6061 aaaaacgaat atatcagaaa ttatacttgg agctactata gttagttttg caacaacatt
     6121 acctgaattt acagtgtctc tacttgcttc tattgatggt catactacta tgagtttagg
     6181 aaatgcagtt ggttctataa tatgtaatac agggttagca ttagggttgg ttgtttttat
     6241 aagtccattt aatgtagata aaaaaatgtt tttttctaag tctttactat taatagtatc
     6301 cgttatagtt ttaattttat tgagtttaga tggagttata acaagaggtg attcattatt
     6361 acttataata atattaatct tttatatggt taataattat agaagtgtcg ttggaaaatc
     6421 tgatactaaa aatagaaata ttaaaaataa cactattaaa gacatatcta ctaaaaaagg
     6481 gaaaaattat agaggttttt ccgtattaga aattgtaaaa atactattac tttttgctac
     6541 aggattgata atgatgataa taggttcaca aatccttata gagagtggag taataatagc
     6601 tagtttttta aatatacctc aaggtatagt aagtttaact ataatagcac ttggaacttc
     6661 tctgccagag atagtttcgt cgattactgc tataagaaaa aatcatcatg aaatatcagt
     6721 aggaaatatt ttgggtgcaa acatattgaa tatagtatca gtaatagctg tatcagcaat
     6781 accaaataac ataccaatac tatctcaaaa tagacaattg gatataccat tcatgatatt
     6841 attattatta atagtaataa taccaacact taaatctaat aaacttagta gaatacaagg
     6901 tatattgatg ttattcacat attttctgta catatcaatt ttgtatttca tgtatattat
     6961 ttaactatac tgtttaaaat attaattgca gaatatccta aaaaagtata taatggatag
     7021 gttaagatta attgataacg gagaggaata atatatgaat ataacaaagt ttgaagattt
     7081 accaataagt gagggaataa aaaaagctat agctgaaatg gggtttgaag aaccatctcc
     7141 tatacaagca cagtctatac ctgcgatttt gtcaggtaaa gacgttatag gacaagctca
     7201 aactggtacg ggaaaaacag ctgcttttag tatccctata ctagaaacaa tagacccaaa
     7261 taatagaagt ttacaagctg tagtgctttg tccaacaaga gaactggcaa tacaagtatc
     7321 aacagaaata agaaagttag ctaagtattc gcacggaata aagacattac ctatatatgg
     7381 tggtcagcca atagatagac aaataaaatc tctaaagagt ggtgtgcaag ttgtaatagg
     7441 tacaccagga cgtacaatag accatatcaa tcgtaaaaca ttaaagatgg ataatgtaaa
     7501 aatgattata ttagatgaag cagatgaaat gctagatatg ggatttagag aagatataga
     7561 aatgatttta agtaagattc cagaagaaag acaaacaact ttcttttcag ctactatgcc
     7621 aagaggaatt ttggaattaa ctaagagata tcaaaaagac ccagagcata taaaagttgt
     7681 tagaaaagaa cttacagttt caaatacaaa gcaatactat atagaaacta ggtcatcaaa
     7741 taaattagaa gtattatgta gattagtaga tgtatatgac cctaaactat ctgttgtttt
     7801 ctgtaatact aagagaaaag cagacgaatt ggtaggagat ttacaagcaa gaggatattt
     7861 tgcagatgca ttacatggtg acttaaaaca gactcaaaga gatatagtaa tggataagtt
     7921 tagaaatggt acaatagata tacttgttgc tactgatgtt gcagctagag gtatagatgt
     7981 tgatgatgtt gaatgtgtat tcaactatga cttaccacaa gatgaagagt attacgttca
     8041 tagaattggt agaactggtc gtgctggtag agaaggtatg tcatttactt ttgtatttgg
     8101 aaaagaaatg agaaagatga aagatataga aagatatact aaatctaaac taataaagca
     8161 taatatacca acaataactg atgttgaaga gaaaaaagta ggtactttct ttgcacaagt
     8221 taaacaaact atagaagaag gtcacttaac taaacaatta caatggttag aaggtttctg
     8281 taatgatgaa gattatgcaa tggttgatat agctgctgca cttgtaaaac tatctctagg
     8341 tgaagaaatg aaagaagaaa taatagaaga aaaacctaga agagagcgtg gagatagaaa
     8401 aggtggtact ggagctaaag atggtatgat tagattattt atcaatattg gtagaaatca
     8461 aagagtacaa gcaaaagata tagttggtgc catagctgga gaagttggaa taccaggtaa
     8521 agtagtaggt actatagata tatatgataa gtatactttt gttgaaatac ctaaaaaaga
     8581 tgcaaaaaca gtaattgaaa agatgaaaga tattaagata aaaggtaata agattaatat
     8641 agaaaaagca aataagaaga aaaaataacg tatagtatac aaaactataa ttagaaaata
     8701 tataaaataa tatattggat atttaaaaag cctgatgtgt tttcaaaaca gatagtagat
     8761 aatctgtaag agttaacagc ataaggcttt tcttgtttta tatgatgtta taattttcca
     8821 gattgttgtg aaaaatgtat attagttcct ttttttcaaa tctgatttga aaaagagaat
     8881 attaattatc tatatagttt agattataat aaaaaatgta agataacaaa ggggagaaaa
     8941 agatgtcaat gacttatatg gaccctaggg tttttttgat tttagaaatg gtgttagaag
     9001 aagacacaaa tactctagaa caattttcaa atgttttggg tgttagtact aggactatca
     9061 gaaactatat aaaccagatt aatcgagaat taaaaggtgg tattgctaaa atagttaaaa
     9121 atgaaaaagg tacatatagt ataagaattg aggatgaaag taaattaatt gagattgtaa
     9181 attttaatag aggaaagcta tcaaatttta taaatttaaa ttcacctgat gaaagaataa
     9241 attatgttct tgatatttta actatgacca acaatccaat taccatagat gatttggctg
     9301 aagagatatg tataggaaga acaacattaa taaaggattt aaaaaaagca gacagaattt
     9361 tagctgaata tgatttagaa ttaaaaagga agccaaatat aggtatgatt ttagttggtg
     9421 aagaaatgga tataagatta ttaatccttg atagattgta tgaaaattat attgatgtct
     9481 tggaaaacgt taatcagata aatttagtta aagattatga tattgaatgt cttagagatg
     9541 aattaaaaaa gttatttaaa aaagaagaac tctacataac agaacaggtc ttgagagatg
     9601 ttgaaagata tataatcatg tctgttttaa gaaatctaaa tgggtacaaa tttgaaaaga
     9661 tagataagag atttgaagtg ataagatgtt cagatgaata tactttagga ttaaagctca
     9721 aagctttatt ggaaaagata tttaatatag ttttaaatga aaaagaaacc atatttttga
     9781 caatgccatt aataggtaga aaagctccta gtacaaaatt agcacttagc agtataaaga
     9841 taaatgatag cgtaaaggaa gttgtagatg aagtaactag ttttttatta gagacctcag
     9901 ggattgattt taaagaagat aaaaagctta tagaaaattt ggaatatcac ttgtattttg
     9961 cacttaatag aatgagattc aacataaggg taaaaaatcc tcttttacaa gaaataaagg
    10021 aaagatatac atttccatat agcgttgcaa agatagctgc aatgataatt gaggaaaagt
    10081 ttgacttgaa aatatgtgac cacgaaatag gatatatagc attacatttt ggaagttatt
    10141 ttgaaaaaaa ttcaagaaaa gttctaaatg taaatagggt agctgttgtt tgtggaacag
    10201 gtcttgggac tgcacaactt attagaataa agataggaaa aatacttgga gaaagtgtag
    10261 aagtaaatac tttttctgat atagaagcta aagctaattt agatttatta aagtattatg
    10321 atttagtatt tacaacagta gatttcaaaa cagacttaga tgttccaatt tttagagtaa
    10381 atgcgttatt taatgaagaa agtataaaga aagagataga aaatgcaata cttttgaaaa
    10441 atagtataaa tcttaagtct ataaaaaaga ttgaaaaaaa tatccctttt ataggtaaat
    10501 taattgaaga ggataagttc tttatattga ataagagtac gtttatggaa aatttagaag
    10561 atatgttaga aaaattagtt cataaaggaa ttattgatat agagttcaaa aaaaaggtat
    10621 taaaaagaga aaagaaatct tcaacagcat tagataatta tatagcattg cctcactcag
    10681 ttaatactaa tggggaaaat ctttatctgg catttggaat attagaaaaa ccagtgattt
    10741 gggatacaaa ggaaattaga ataataatat tgatgataat tccagataag aatgtagata
    10801 gtacagacct gataataaaa agttatgaag aagtacttga agtagggaga gataaaaaga
    10861 tggtagaaga acttgctaag gtaaaatcat ttgatgaatt ttataaaata attaccaaga
    10921 ggaggtaata atatggaccc agtaattttt attatagcat cacttattac agtgtattta
    10981 ttaaatattt ttcttggata cttacagctt aaagatttta ataaaaatta tattgaacta
    11041 aaaagaaaag gaagagtggc aattggcaga aaaaaaggaa gaatttcatc gggtacaatt
    11101 gtgttgatac ttataaatga tgatggtgtg attgtcgaga ctagaaaaat gcaaggtgtt
    11161 acagtattgg caagagtaaa gtcatttgaa ggtttggtag gaaagagctt agagtatata
    11221 gaaaaatcag atttaactgt ttacaataaa ctacttaaaa aagcaatact agatgcagtc
    11281 aaacagtaca taacatttga gaaaaataaa tgtgaagata caaataatct gagtactgat
    11341 gaagagtgta ggatggaagt agtttaaaaa aatatattta taaggaggag ttaatatgac
    11401 gcaatttttt caagtattag caagtggtgc agatagtttt atgaatttat ttagggcagg
    11461 tgggcaacaa tttgtaggtt ttgtaacaga tatagtacca cttttagtag cattattagt
    11521 tacaatgaat gcaattatca actttattgg tacagataga gtcgagagac ttgcaaaaaa
    11581 atgttctgca aatatattta ctagatattt gatattacca gtccttggta catttgtatt
    11641 tgcaaaccca atgacactat cattaggaag attcttgcca gaaaagttta aacctagtta
    11701 ctttgcagca gcaagctttt catgccatac aatgaatgga ttattcccac atatcaatcc
    11761 aggtgaacta tttatatatc taggaattgc aaatggtata acaactttgg gattctcaac
    11821 agcagattta gcagttagat atttgttagt gggtatagtt gcaaacttta ttaaaggtgt
    11881 agttactgac tatacaacaa aatttgtaga gaaacaacaa ggaatagcat taaattcaga
    11941 tataaagatt ggataattac tttaaggtat aaataaattt ttcaaataaa aagatagtgt
    12001 atagaggagg ataaattata tggcatttaa caaagttaaa atagtaaaag gttctggagg
    12061 atggggtgga ccattgataa tagaaccaac agaaaagaag aataaggtag tatatgtgac
    12121 tgggggagca aagccagaaa ctgcagtaaa aatagctgaa cttacaggat gtgagttaat
    12181 agatggattt tcacagggtg ttaaagatga tgaaatcgca tgtgtaataa taaattgtgg
    12241 tggaacattg agatgtggta tatatcctca aaagaaaata ccgactataa atataatgaa
    12301 aacaggaaaa agtggaccat tagcaatgtt tataaaagaa gatatatatg tttcaggggt
    12361 aaaaccaaca aatgtagagc agttataatt gaggtagtag gagggaagaa atatggctta
    12421 tgatactaaa aagaagataa gtgagcagag tcaaggaatt gttgcaaaaa taggtatagc
    12481 actaggaaat gtgacaagta ctctttttca agctggtagg gatactatag atacattaat
    12541 acatactata ctgcctttta tggctttcgt tgcagtccta ataggtataa taaatcaatc
    12601 gggtatagga aattgggtag ccaatttact tactccatta atcggaaatg ttggaggttt
    12661 agttgtctta tcaataatat gttcatttcc gttgttgtct ccgtttcttg gaccaggagc
    12721 tgttatagct cagatagtgg gagtattggt tggtacagaa ataggaaaag gaaatattcc
    12781 accaagttta gcattaccag cattgtttgc aataaactca caagcagctt gtgactttat
    12841 accagtaggt cttggtcttg cagaagctga acctgataca gttcaagtag gagttccgtc
    12901 agttttatat tctagatttt taacaggtgc accaactgtt ctattagcat ggttagcaag
    12961 ctttgggtta tataaataga agaatacttt ataaataatt agagagaggt tcagttgata
    13021 tggaattaat atacagtact caaataaata aaataggaga taatgcaaat gaatttttac
    13081 aacataatat gtttgtaact ttcaaagata atgctcctca agagttagaa aattactgtt
    13141 atatacattc tgaaaataat ttagtaaaag aaatacttac ggatgatgtg ctatcaataa
    13201 ataatgttga ttacaaaatt acttctgttg gtgagttagt aaaccaaaat ctttcagaat
    13261 taggtcatat aacatttaag tttacaggtg aaaaagatgg agcaattgga ggaaccttat
    13321 atcttgaaga aaaagaaata gcacctattg gcgtaggtac tatactaaaa ataattagaa
    13381 aataaaattt aaaacgaatc taatcatatt ataagtaaat tatagataaa agtcaattta
    13441 actaatcaag ttaactttta atgtgataaa aaaggggaaa tgaaaatgag aaaagtggct
    13501 ttagtcgttg gaggaggaag aagtttaggt gaatatttat ctaaacattt agctaaagta
    13561 ggttatgatg ttgctattgc agatttaaat gaagacaacg caaaaaaagt agctgaagaa
    13621 ataaaaactg aatttggttg taaatctgta ggatatagtt gtaatgccat agatgaaaga
    13681 caggttataa gtactgtcaa agctattgaa aatgattttg atagaataga tttattagtg
    13741 tacaatgcag gagtggctgt aagtaaaaaa ataactgatt ttggatacaa agattttaaa
    13801 tttattgtag acgtaaattt aaatgggtac tttctatttg caagagaagt atctaaagtc
    13861 atgatagcaa aaggtattga gggtagaata atacagataa attcaaaatc aggtagagtg
    13921 ggaagtaagc ataattcagg atattcagca ggtaaatttg ctggagtagg acttactcaa
    13981 agtttagcgc tagatttggc agaacataag ataactgtaa atgcactgat gctcggtaat
    14041 ttacttgatt ctgatatgtt tgaaagttta attcctcaat atgcagtaaa attgggaata
    14101 cctgaatcag aagtcaagca agtttatata gataaagtac ctttaaaaag agggtgtaga
    14161 tttatagatg ttgcaaatat gcttacattt tatgcttcaa aggaagctga atactgcact
    14221 ggtcaatcta taaatattac tggtggacaa gttatgtaag gttttatgga gtgtctcaag
    14281 atgaactctt tagttcatga ggcacttttt tgtataaaat taaatatatt ttcagcattt
    14341 aaacaatgaa aaagaggttt atctttattt tgagatagcc tcatttttgt aattaaaaaa
    14401 catagtgttt aagtaaacat ataagattgt tagtttttta aaagagaatt ttttaataat
    14461 attatattta tgaagtattt ttaaacttaa tttgataaaa tatagctatg ttttatatac
    14521 tgtagttttg agcatagaaa gtttaataaa ttatagtata tatgttaatt tttaataagg
    14581 taagaaagta gaaagaagga tattttatgg atataataaa aaaattaagt catgggtgta
    14641 cattagattg tcatgactgt tgtaaattca atgtttatac taaaggaaat aatgtagtaa
    14701 aaattgaagg agataaaaat catccttata caaaaggttt tatatgcaaa aaagggatgg
    14761 cccatttaga tagattaaat cataaagata gaataaagac tcctatgctt aaagttgatg
    14821 gagtatggaa agagatttct tttgataaag ctatagaaat aatggcagaa aagcttacat
    14881 actataaaga aaagtataca tcaaagtctg tcatgcatta tgaccaatat ggaagtgggt
    14941 ctgtgctaaa atacatagga gatatatttt ttaattttta tggaggtgtt agtagacata
    15001 aggggggtcc ctgttggagt gcagggatgc atgctcaaaa atatgatttt ggagtagcta
    15061 agagtcatgc tatagaagat atgttaaata gtaaaagtat atttgtgtgg ggaaaaaatc
    15121 cagcatatac tacaattcat actatgcaaa taattaaaaa agctaaagaa aaagggataa
    15181 agatagtagt gattgacccc atatatacaa aaacagctca aatagcagat aagtatgtac
    15241 aagtaaatcc tggaacagat ggagctttag ctatagctat ggcaaaaatt atagtagaag
    15301 ataaattata tgatgaagaa tatataaact cgtatgttat tggttttgaa gaatataaaa
    15361 aatatttatc ttcattggaa ttaagttttt taattgatga atgtggtgtt aaagagaatg
    15421 atataagaga gttagtggat ttatatacta acaaatattc aagtataaat gttggatatg
    15481 ggcttcaaaa atataaaaat ggtggaaaca caataagagc aatagatgct ttaggtgcta
    15541 taactggtca aataggattt agtggtggag gggtaaacta tgctaataaa gtttatccga
    15601 gtgttataaa ttctgaccct tacaatagcc aatcatatgg tgaagatagg gagttttatg
    15661 taagtaatat cagtaaattt atagaagaat ctttaaaaaa tacatcaaat aaagtaaatt
    15721 atgcatcaga tgaacttgat atggcattac ataaagtaaa ttacatatca gatgaacttg
    15781 atacaacatc aaataaagta aattatgtat cagatgaact tgatataaca tcaaataaag
    15841 taaattatgt atcagatgaa cttgatatga catcaaataa agtaaattat gtattagatg
    15901 aacttgatat aacatcaaat aaaatagatt atatatcaaa tgaattgtat aatttatcta
    15961 ataagtcaat caaagataat attcctataa agatggctgt gataactaaa agtaatatgc
    16021 tgaatcaact gccagattta gtagaattgg agagggtgtt ttcaaaaatt gaatttaaag
    16081 tatgttttga tatgtttata actgatacag ctactttatg tgacatgttt ataccatgta
    16141 caaatacact agagagtgaa gatataattt atagttcaat gaccaatcca tatataacct
    16201 ataatgaaag agctgtaaag ccagcacata aattgatgga tgaatattac ttctttatgg
    16261 agctagctaa aaagatggga ttaaatgatt atccttttgt agaaaagaga acttatttag
    16321 aaaaagtgat tgaaccactt aagcgttttg acaaaaatct ggatatagaa aaacttaaaa
    16381 acaattactt tactatacat aaccctgttg cttgggaaga taagaagttt gagactccat
    16441 ctggaaaata cgagctttat tcagaaagta taaaaaactt gggaataagt ccaacacctg
    16501 tatatataag caataaatat aaagaaatag aagataaaaa tatttctttt agattattaa
    16561 ctaatcatca tgcagatact ttatttagtc agcattttat ggacaaaaaa tctatagctc
    16621 aagcatatat aaatcggaga atggctaaaa aagtaggtat tgaggacaaa gatattgtga
    16681 tattgaggtc aaaaaaagca aaaataaatg tacaaataaa tatagatgat ggagtaggta
    16741 attatatagt aaaaatgtat gttggttggt ggaagaaaca tggaaatcct aatagtttaa
    16801 cagacactgg tatatcagat tttgggggtc aggtaacata taatgaaagt atggtagaaa
    16861 taatacgaca aaactagtta aatttcattg aaggaataaa aatataatta tagaattgat
    16921 taaaagttac tatttttaca aaactattga aggagggatt tttgatggta aattattcct
    16981 tagaacataa gaatctatac atagaattta tgtgctcaga actagaccac catgtagcca
    17041 atgaaataag ggaagaaata gataacttat taagtgtaaa tcaagtaaaa aatgttgtat
    17101 ttaattttga aaatattaat tttatggatt cttcaggtat aggcgtcatc ataggtagat
    17161 ataaaaagat ttctaatgaa gggggacgag tttcagtaat taatataagt tcacgagtta
    17221 agaagatttt tgatttatct gggttaaata aaattattgg catttatgat acgtatgaag
    17281 aagctctaag ttctttgtag ggaggagtat gtgatgaata atattatgga agttaaattt
    17341 tcggcaatat ctgaaaatga aagtttagca agagttatag tagcatcctt tgcagctaag
    17401 ttagacccaa cagtagatga gttagtagat ataaaaactg ctgtctctga ggctgttacg
    17461 aatgctataa tacatggata tgaagaagac agttctaagt ttgttttctt aagatgtgag
    17521 atagaaggaa atactataaa gattatagtt gaagatgaag ggtatggaat agaaaatgtg
    17581 gagaaggcaa tggaaccact ttatacatca aaaccagaac ttgatagatc aggaatggga
    17641 ttcacagtaa tgaaaagctt tatggacgat gtagaggtaa gttctgtaaa agataatggg
    17701 actagaattg aaatgactaa aaaaataaat gtgcctaaat aaagtccaaa agtgaggttt
    17761 tattatggaa gtaactgttg ccagagaaga aaaaaaacct cttcttagtc atgaagagac
    17821 tctagaactt attgaaaaag ttcaaaatgg agatgaagag gcaaaagaaa tattaatatc
    17881 aagcaattta ggtctagtta ggagcgttgt atctagattt ttaaatatag gatatgatag
    17941 agaagattta tttcagttag gttctatagg cttaataaaa tcaatatata aatttgaccc
    18001 aaaatttaat gtaaaattct ctacttatgc agtaccaatg attttaggtg aaataaagag
    18061 atacttaaga gatgatggta tgataaaagt atcaaggtca ttaaagcaaa tagctgtaaa
    18121 ggcaaagatg gaaagtgaag ctttaactaa aaaattaggt agagagccga gtatagaaga
    18181 attagctaaa gcaatagatg tagaaaaaga agatttagta atggctatgg aagctaattt
    18241 caatgttgag tatttgcaag gagttattca tgaagaggaa ggttctccaa tatgccttat
    18301 tgataaaata agcatgaaag gagaaagtga agaagaaaaa gtagtggata atatattgtt
    18361 aaaagacata cttggaagac tggataagag agagcgtcaa ataatagttc tcagatactt
    18421 tgaagacatg actcaaagtg aaataggaga gatgctcaat atatcacaag ttcaagtatc
    18481 taggatagag aagaaagtat tgtctaagtt aaaagaatat atatcgtgat aatacttatg
    18541 atatgtagaa taacaaaata taataaatat attactaaaa taaaatgtaa taacttttta
    18601 ttttaagttt ttaaattatt aaaagatatt aaaataataa aaagcattaa aaaagtaaaa
    18661 atataaaaac aaggtgtctt tatggaaaaa tagatttttt ctttattagg catctttttt
    18721 attttttgac caaactttag atagtcaaaa taaataatag gtttattttt ttaaaattaa
    18781 caaataatac tttagagagg tgtttagtat ggataaaaat tataaaaaat atgtagacca
    18841 aataagccca aaacctacat atttaaaaaa ctatacactt gcatttattg taggtggaat
    18901 tatttgtatg atagggcaag caataaatga tttgtatatg aaagttggag gtcttgataa
    18961 actaggtgct agttctgcaa catcaataac attaatattt ataggggcat ttttaactgg
    19021 attaggagtc tacgacctaa taggaaaaag agctggagct ggttctataa ttccaataac
    19081 aggttttgct aactcaatag tatctccagc aatggagtat aaaagagaag gctatgtatt
    19141 aggtgtaggt gcaaacttat ttaaaattgc aggaccagtt ttagtttatg gtataggttc
    19201 ttctatttta tgtggaatta tttattatat ttttaagatg ttttagatga tttttattta
    19261 atttaaattt tattaattat gaagaaggta taaaatctga aaatagctac taaagtaact
    19321 aattgaaaaa taattgtgca gatttaatta atattaaaaa tttgaatagt aaaatgcaaa
    19381 attaaaaagg tgtgaaaata tgaaaaataa aagaattgga aaaagaacag tcaagttaga
    19441 aaataaacca accataatat caactggaac tatagtaggt cctaaggaag gtgaaggacc
    19501 actaaaggac tattttgata tgattatgac tgacgattta tatggtgaaa agacttggga
    19561 attagctgaa agtaaaatgg tagagactgc atctcaacaa gctatccaaa aggcaggtaa
    19621 aaaattgtca gatgtaaatt atatgctagg tggcgattta ataaatcaaa tagttccagc
    19681 atcatttgca gcaagagaac tggctattcc atttttaggt atatatggag cttgttctac
    19741 aatggcagaa ggtctttgta taggttctat gcttatagat ggtggttttg ctgatttagt
    19801 actgtcaggt acatcaagtc actattgtac agcagaaaga caatttagat tcccacttga
    19861 acttggaaat caaaaaccaa tgacagctca gtggacagtt acaggagcag gaagtgtatt
    19921 attagctcct aatggagacg gtccaaaagt aaagtatgtt actgttggta aagtaataga
    19981 tgagggtatt gatgatggta ataatatggg accagctatg gctccagcag caatagatac
    20041 tatctactct tactttgaag atactaatga tgacccaaat agctttgata taattgctac
    20101 tggagattta ggtactcttg gaaaacagat agcagaagac tttctaaaag aaaagggtgt
    20161 agatatatca aaagtttata ctgactgtgg tatagagatg tttaacctta aagaacaaga
    20221 tgtccactgt ggtggaagtg gatgtgggtg ttcagctaca gtgtttgcag gatatatata
    20281 tgataagttg agaaaaaaag aatttaacaa agtaatgctt gtatcaacag gagcactact
    20341 ttcaccgaca agtacactac aaaagcagac aataccatgt gttgcacatg cagtagtaat
    20401 agtaaatgag taggaagtga tataatatgt taatggacta tgtaagagta tttattgtag
    20461 gtggaattat atgtttaata gcccaagtaa tgatggatta tttcaaaatg caaactccat
    20521 atgttatggt aacgtatgta acaactggtg tagttttaac ttttctagga ctttatgagc
    20581 cattagttaa gtttgcagca gcaggtgcaa cagttcctat aataggattt ggatattcac
    20641 ttgctacagg agtaataaaa tcaattcaaa gtgatggttt tttaggtatt tttttaggag
    20701 gtacaactgc tacagctggt ggaatagcag cagctatatt ctttggatat attatgtcag
    20761 tagtatttac tccaaaagca aaaccataaa tctttattaa aagaataata agtttttgaa
    20821 ataataactt tttataaagc aggtggatat cttttagatt ccacctattt tttatataaa
    20881 gaagcgttta cataattgaa gttgacaatt atttacaaca tagtgtagta ttatattatc
    20941 aaaataaaat gaagtgaagt aggggataaa gatggtaact attaaagata ttgcaaggaa
    21001 tctaggtatc agttattcaa ctgtttctag gtgcttaaac aataatccta atgtatctga
    21061 aaaaacaaaa aagaaagtgg tagaagaggc aaatagacta gggttccatt ttaatgtaaa
    21121 tgcaagaaat ttagcaaaaa aagaaactaa caggataggg gtaatctttt caaataattt
    21181 taatcatcaa gatactagaa agttttttag tgatataatg gatagttcta taaattctat
    21241 tgagactaat aaatacgatt ttataataca gcctaataat aacattagtg gtgatagtaa
    21301 tgtatataaa atggtaaatg gacaaatggt tgacggatta gtaattgtgt ctcaatcaat
    21361 aaaaaaagaa gagtatgatt ttttaaaaga caataatttt ccacatgtat ttatatattt
    21421 taaaccatct tttataggag aagtagataa tttcttttgg gacgataacg tgtatggagg
    21481 ttatatagct accaagcatt taattgacca tggtcataaa gatataataa cagtaacttc
    21541 agatgataag agttcaaaaa tgcatgaaga tagaaccaag ggatatttga atgctatgga
    21601 agaagcaaat ttaaagacag aagtcattaa atctaaaatg gattttgaat ctcaagttga
    21661 atttttagaa aaatacattg ataaaataaa aaaagcttca gctatatttg tacaacaaga
    21721 tgtaccagcg atttctataa tacaagagtt aaaaactact tatggtataa atgtaccaga
    21781 agatatttcc ataatagggt ataataatat tgaattaata tcatatttta ggtcgcattt
    21841 atcaacaata gatgacccaa gagagcaagt tataaaaaat ggagtagata gtttagtaaa
    21901 tataataaat aaaaagagta cagaacacat tccgagaaaa ttatatccaa ggttaataat
    21961 acgaaatagc gttaaaagaa taaagtagta agcttttaag tatcaaacgt ttattaaaaa
    22021 tattatagat tatgtgaagt tttttaacaa ataatataa
//
LOCUS       DAELNY010000049        21629 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.049, whole genome shotgun sequence.
ACCESSION   DAELNY010000049 DAELNY010000000
VERSION     DAELNY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.049"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..721
                     /locus_tag="KPE55_001608"
     CDS             92..721
                     /locus_tag="KPE55_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492697.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            722..1504
                     /locus_tag="KPE55_001609"
     CDS             722..1504
                     /locus_tag="KPE55_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492698.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1497..2375
                     /locus_tag="KPE55_001610"
     CDS             1497..2375
                     /locus_tag="KPE55_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492699.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2408..3367
                     /locus_tag="KPE55_001611"
     CDS             2408..3367
                     /locus_tag="KPE55_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0492700.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3681..4373
                     /locus_tag="KPE55_001612"
     CDS             3681..4373
                     /locus_tag="KPE55_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0492701.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4383..5387
                     /locus_tag="KPE55_001613"
     CDS             4383..5387
                     /locus_tag="KPE55_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0492702.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5635..6486
                     /locus_tag="KPE55_001614"
     CDS             5635..6486
                     /locus_tag="KPE55_001614"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0492703.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6590..7336
                     /locus_tag="KPE55_001615"
     CDS             6590..7336
                     /locus_tag="KPE55_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492704.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7336..9252
                     /locus_tag="KPE55_001616"
     CDS             7336..9252
                     /locus_tag="KPE55_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492705.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNIKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICRKFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9531..10448
                     /locus_tag="KPE55_001617"
     CDS             9531..10448
                     /locus_tag="KPE55_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0492706.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQCNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEISVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            10526..11263
                     /gene="cas6"
                     /locus_tag="KPE55_001618"
     CDS             10526..11263
                     /gene="cas6"
                     /locus_tag="KPE55_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0492707.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11269..13146
                     /gene="cas8a1"
                     /locus_tag="KPE55_001619"
     CDS             11269..13146
                     /gene="cas8a1"
                     /locus_tag="KPE55_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG0492708.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKEENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLINNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIGGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQMPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            13148..14062
                     /gene="cas7i"
                     /locus_tag="KPE55_001620"
     CDS             13148..14062
                     /gene="cas7i"
                     /locus_tag="KPE55_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG0492709.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFFSVDKFFGQLVKEVKEYYGVN"
     gene            14064..14765
                     /gene="cas5b"
                     /locus_tag="KPE55_001621"
     CDS             14064..14765
                     /gene="cas5b"
                     /locus_tag="KPE55_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG0492710.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14776..17097
                     /locus_tag="KPE55_001622"
     CDS             14776..17097
                     /locus_tag="KPE55_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0492711.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDLVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            17112..17663
                     /gene="cas4"
                     /locus_tag="KPE55_001623"
     CDS             17112..17663
                     /gene="cas4"
                     /locus_tag="KPE55_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG0492712.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     SKIEKKHYCTKCAYYEFCFVKEE"
     gene            17666..18646
                     /gene="cas1b"
                     /locus_tag="KPE55_001624"
     CDS             17666..18646
                     /gene="cas1b"
                     /locus_tag="KPE55_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG0492713.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18649..18915
                     /gene="cas2"
                     /locus_tag="KPE55_001625"
     CDS             18649..18915
                     /gene="cas2"
                     /locus_tag="KPE55_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG0492714.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19099..20835
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19099..19127
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            21024..21452
                     /locus_tag="KPE55_001626"
     CDS             21024..21452
                     /locus_tag="KPE55_001626"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG0492715.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT         8828 a         1952 c         3722 g         7127 t
ORIGIN      
        1 ttaagaaaaa attaatatgt tgacaaaatt taagtgctca tttaaactat taattgataa
       61 taatattcaa tattaaataa taggaggcgt tatgaataaa tatcttctaa taaatccagt
      121 ggcagaaaga atgtatggag aaaagtttca ccttattaaa gcaaatttga taaaaaaggg
      181 gtacataata gcagaatgtg aaccacagtt agaatatgta aaaaagcagt acaaagagta
      241 tacaaaaaag actaaaaata caatgttgga ttgtagatgt ccagaaagta taagtctttt
      301 aaaaagaaat aatttgataa atgattttga agtacctatg atagaaccaa tattagtaag
      361 gacttgtagg gttttatacg ataaatatat aaaaagtaaa gatgatatgt tggtagtaac
      421 ctgtccatgt acacaattaa gagactttgc aagtgaaaag tttaaggata agggtaatgc
      481 tatgttttat acatggaaag agtttgctga acaagaaggt gtaaaatcac ttggaaaaat
      541 agatgaatca ccaattccat taggatattt caaagatact tttgaaaaag ttcttgaagt
      601 atcaagtgaa gacgaaatat taagtgaaat acaaaaaata agaaatggtt ttgaagataa
      661 gtatgacata gtagaaatgt tttattgtaa agatggttgt aataatggag atggtttgta
      721 aatggtcaat aaatttaata ataagttttt aaggctagta aagagtgttc tgatattttt
      781 tataattctt ttattatgga aaattacaaa ttatcttggt atttggagtg attatatatt
      841 accatcaccc gaaaaggtat atagtacatt tttaaatatg ataagtgatg gttcaatatt
      901 tattaatgta tatgcaagta tgaaaagagt tttgataggt tttgcaataa gtactgctat
      961 aggtgtgcct ttagggatat tctttggaat ttatagtgga gtatacgaat actttaaatc
     1021 tctaataaat tttttaagga atacacctcc ccttgcttta ataccaatgc ttatattatg
     1081 gtttggtata ggagaggaat caaaaattat aattatagtt ttagcatcct ttttcccaat
     1141 ctttacaagc acattgaaag ggattaaaaa ttgtgactca aaactgattg aagtgggtag
     1201 agtatttgag ttttcaaagc ttcagataat atttaaaata ataataccaa atgccattct
     1261 tgatattgct gttgggttaa agttagctct tggatatagc tttagagcaa ttattggggc
     1321 agaacttgta gcagcttcat ctggtcttgg atacttgata tctgatggaa aagaaatgtc
     1381 tagaacagat gttgttattg taggtataat cgtaattgga cttttaggaa taattacaga
     1441 ttacatattt tcgattattg tgaagaaggt aagtaaggga aagatggtgg aagcatatga
     1501 ataatttagt atgtgactct ggatatataa ttagaaattt atataaaagt tataaagttg
     1561 acaatgaaga acatgtagtt cttagtgata tctctttaaa catagataga aaccatataa
     1621 cagtaatttt aggtgaaagt ggttgtggaa agactacctt gcttagagca attgcatcac
     1681 ttgaaggtat aaatagtggt gaaattaggt ttattaatga taataaagag tatagaccta
     1741 atgttggttt tgtttttcag gagagcagat taatgccttg gttaaacgta agtgaaaata
     1801 tagcttttca taatcaaaga aaaaatacta ttttaaacaa ggtgcttaga aaatttaaac
     1861 acaataaaag aattagcagt aaaattataa atgataattt tagaagctta gaaacaaatg
     1921 ataatttgaa caaatcaaat aaatataata ggaaaagtaa tattgatgta gaaaaatatc
     1981 ttaagatgat gaacttgcag aaatttaaaa actcttatcc aaatgaattg tcaggaggta
     2041 tggcacagag agtaagcata gctagagctc tttctttcaa tccagatatg ttgcttatgg
     2101 acgagccttt ttcggcatta gattatttta caaggattga tatgcaaaat gaagttataa
     2161 gaatacataa gtcaactaat aaaggcgtga tatttgtaac tcatgatatt gatgaagcgt
     2221 taaagatagg caagaaaata gttgttttca cagacgaaag aaaaataaaa gaatttaata
     2281 tagaagatgg ttataataga gatttaactt cagattacta tattaaatta aaaaaagata
     2341 ttttaatgac aatgaaatca aataaaggca tttaaagaaa ttaataatat tttaaggagt
     2401 aaagtaaatg aaattaaaaa agctattgat aggtataacg atattaacac ttgcaacagc
     2461 atctttagct ggatgtgcta aaaaaaataa tggagaaaaa ctttcagaaa taaatttaac
     2521 ttatgtaaaa tcaccattaa atgttccttc tataattcag aaacaggatg atttatttgg
     2581 aaaggaattt aaaaaggata atataaaagt taacttccac gaaattacta caggtccaga
     2641 gcaaacacaa gctttagctg ctggagaaat agacttctta catgcactag gtggtacatc
     2701 agctttaata gctgcatcta atggagtaga gttaaagata ttaaacactt atagcaggtc
     2761 accaaaagga tttatgattt tgactaataa taattcaata aaatcagcag cagatttagt
     2821 aggtaagaag gtggcaggcc caaaagggac tatattacat caggtattga tttcagcact
     2881 tgataaagaa ggattaagta tggatgatgt ggaatttgtt aatatgggta ttccagaggc
     2941 atcagcagct ctttctgatg gcagcgtaga tgctgcattg attgctggtc cagcagctct
     3001 taaagctatg aagtcaggta gtaaacttgt agcaaatggt gaaggtttag tagatggtat
     3061 aatagtaact gctgttagca cagactttgc agagaaacat ccagaaatag ttgaaagatt
     3121 tatgaaggtt gaaaaggaaa ctttagaata tgtaaataat aattttgatg aagctatgga
     3181 aaaagtagct aaagaagttg atttaagtct tgaagaaact aaagaattat atgcttggta
     3241 tgattttagt ttagatataa cagataaaga catatcttcg cttgaagata cacaagactt
     3301 cttaataaaa aataagttac aagagaaaaa agtgaacata aaagaattaa tctataatgc
     3361 taagtagaaa ctatgataaa ataatactaa gtaagaaata taataaaagt ttaagttacc
     3421 ttattaaggt aactttttct attttgaatt agtaatttca atttatttta gaatgattag
     3481 ttgataataa tgattaatta atcataaaaa gatacttttt tatagagttt tattaagatg
     3541 tctaataata tggttatact tatgaagtca gtaattgtga agttagtaat atgattaaat
     3601 ttatatattg caaaaagatt ataatatatt aaaattggac tgttatatga tgagtaaata
     3661 ggtatattag gaggaattat atgagttata aagtatatat tgtggaagat gatatatcaa
     3721 taagtacatt acttaaagat tatattgaga aatatggatt tgaagtgatt gttgaagaga
     3781 actttgatga tataatgatt acatttgaaa gttttaagcc acacttagtt ctattagatg
     3841 taaatttacc taaatttgat ggtttctact ggtgtagatg tattaggcaa cagtcgaatt
     3901 tacctataat ttttatatca gctagagatg gtaatgtaga ccaaattaga gctttagaaa
     3961 gtggagcaga tgattatata acaaaacctt tttactatga tgtagtaatg gcaaaaataa
     4021 agagtcatat aagaagggta tatggtgaat attcacctaa aatagacgaa agaatagtgg
     4081 agctagatgg attaaagttt tatccagaaa ggttagaact agaattaaat ggtgagaatc
     4141 taattataac taaaaaagaa ggtatactga ttgaatgtct tatcaagaga tacccaaaag
     4201 tagtaagtag agattatctt ttagaaaaga tatgggatga tattgagttt gtagaggaaa
     4261 atactttaaa tgtaaatgtt agcaggataa ggaaaagatt tgaggaatta ggtatagata
     4321 atgctgttca gactgtccga gggttaggat ataaattaaa taagaattgg taaggagaaa
     4381 gtatgaagtt atttttaaaa gattataaag gttatatttt tatatattat ataggcatag
     4441 ttatgacaat tatttactgt aatcttatga agtttattag ttttggagag agtttatata
     4501 ttcttttatt tagttctttt atattgttat gttttttaat atataagtat tataaaaata
     4561 gagaagtata taatctgttt gaaaaaggag tacattcttt ggaggagtca tttttgtatt
     4621 taggaagctc attcttgggg agaaatatat cagttatttt aaaacaacaa cataaattgt
     4681 atcaatcaat gatacaagag cataaaaaga tacatagtga acatttaact ttcatcaacc
     4741 aatggattca tcagatgaaa acaccaatat cagttataag ccttcagctt caagattatg
     4801 agggagaaga agtaactgaa aatataagaa aagaaataga tagattaaat aaagggttag
     4861 atatggctat gcattttgca agggcagata attttcagaa agactttata gttgaaaaag
     4921 ccaaattaaa tgaaattgta tctagtattg taaatgaaga aaaaaggtta tttataaaaa
     4981 gtggaatgat tcctaagatt caagtagaca aatttactta tgtttataca gatgtaaaat
     5041 ggatgaaatt tataataggt caattaatta caaatggagt gaaatattct aaagattata
     5101 gtaactacct aactataaaa tctgaagaaa ataataaata tgtaatttta agtataatag
     5161 atgaaggaat tggaattgtt tctaaagatt taaaaagggt atttgaccca ttttttacag
     5221 gagaaaatgg tcgtaagttt ggagaatcta caggtatggg tctttatcta gtaaaaaagg
     5281 tatgtgatga tttaggtcat aaagtaagta taaaatcaga agtaggaaaa ggtacagaag
     5341 tttcaatcta ctttaataaa gatgtaaata gtttaataaa ggtgtaaatt atctgttaca
     5401 atagggtttt tagttaattg tactttttta ttatacaatt tattaaatga aatattcata
     5461 gttaaataat caaaatttaa gagagtttat ttgcaaacct tacatagatg taaggtttgt
     5521 gtaatgtttg cagatagtta aattttctaa tctaaattac aattaatata agaaataaca
     5581 taagagaaat aacataaaaa aagaaatttt gtaaaaaagg aaggatgttt attgatgagt
     5641 ttagatgtta tttttatatt gaaatcagtc ataatagcta tagtagaggg gcttacagaa
     5701 tttattccag tttcatctac aggtcatatg attttggttg gtaatttaat agattttaag
     5761 gggcaatttg ctgaaatgtt tgaggttgta atacaacttg gggctatatt agcagtagta
     5821 gtactttatt ggaaaaagat aaaagatagc gttatagaat tttttaaatt tatatttact
     5881 ggcggaaaag aaggaaagat tggttttaga tttggaatga atgtaattat aggttgtata
     5941 ccatttgcga ttataggagt tctattttac gataatataa aatcattgtt taatttacaa
     6001 tctgtaatta taggatttat agtgggtggg attttattat tagtagtaga aactttgttt
     6061 agaaaaaaga atcattccac agataatata gataaaatta ctccaataca agctttaaag
     6121 gttgggacat tacaagtttt gtctgcatgg ccaggtatgt caaggagtgc atctactatt
     6181 atgggtggat ggatagcagg acttaattct cctacagcag ctgagttttc tttcttttta
     6241 gcagtaccag ctatggtggc ttcttcagga aaagatttat ttgaatttga ttattcaata
     6301 atgacaccaa cattgtggat tgctttggta gttgggttta tagtagcttt tattgtatca
     6361 ataatagtta tggaaaaatt cgtgaatttt ttaaagaaaa aaccaatgag agtgtttgct
     6421 gtatacagaa taatcatggg tgtagtactt gcagtattag cttttactaa cataattagt
     6481 gtatagaaaa aatagattta ttatctataa aaattaaact aagataaatt ataattaaca
     6541 attatatata tgttatttca aaaattaaat taggaaggat agttaaataa tgaaaatttt
     6601 aactgtaaat aatttaagta aagtatatgg taaaaaaata attttcaacg cattaaatga
     6661 cataaatttt agtatagaag atggagaatt tgtaggaatt atgggtccat ctggtagtgg
     6721 aaaaactact cttttaaata tgatttctac tattgacaag ccaactacag gaactatgga
     6781 attaaagggc aagaatcctt tattgttaag aggtgaggaa cttgcattat ttagaagaag
     6841 agagttaggt tttgtatttc aggattttaa tttattggat acattaacta taggagaaaa
     6901 tatagtattg ccattgacat tagataaggt cagtgtaaaa gagcaagatg agagattgaa
     6961 tgaagtatct acaatattag gtataaaaga tttattagga aaaagaactt ttgaagtatc
     7021 aggtggtcaa gctcagagga ctgctattgc tagagcattg attaacaatc catcaatact
     7081 tttagcagat gaaccaacag gtaatttaga ttcaaagtca tcaaaggttg taatggagct
     7141 atttcaaaag ataaataaag aaaataaagt tactactatg atggtaactc atgacccatt
     7201 agcagcgagc tactgtagta gaatcttatt cataaaagat gggtctatat ataatgaaat
     7261 atataagggt agtagtagag agcagtttta tcaagagata atggatgtac ttacattatt
     7321 gggaggcgat aactaatgaa tataaaacag tttgctatta acaatatttc aagaaatatt
     7381 aaagcgtatt taggatactt agcaagtatt gttatatcat cttcactgct attttctttt
     7441 attatgttta caagtcatcc agatttagat gtatctcaat ttcctgatta tttaaaagaa
     7501 ggtctcaaga tgagtaagat aattgcatat ctattcttat tcttctttgt gttttattca
     7561 gttagtgtgt ttcttaaaag tagatataaa gaatttggaa tactttatat tacaggtata
     7621 tcaaaacgcc aagtaatgaa attaatcttt atagaaaata tgataataaa tatagtatct
     7681 tctgtaattg gaataataat aggcttaatc tttgcaaaga tatttttagt ggttatgtct
     7741 acttttttag aattatcacc attaaaattc tatataccat ttaatgcgat attgtctact
     7801 ttaatctatt ttatgatttt agctgtatta acatcaatat ttacttcttt tatagttaaa
     7861 gaaaatcagg tgttgaagtt acttaaaggt acaaaaactc caaaaccaga gcctaaaaca
     7921 tcactaatgc ttgctatatt aagtatttgt ttgtttataa tagcgtatta taatgctatt
     7981 acctcaaccg accaatatac aacatatctg cgcttagttc cagttacatc ccttactata
     8041 gtagcaactt acttgttctt ttctcaattt agtgtatttt ttatgaagaa gctaaaatta
     8101 aacaagagat tttatagaaa aaatacaaat atgctttgga tttcgaactt gatttataag
     8161 gtaaaggata atgctagaat atttttcttg ataactataa cttctgcagt agcatttact
     8221 gctataggta cagtttattc cttttggaaa gatgtagagc gtcaaattaa tttaatatat
     8281 cctaacacaa tatattattc aacaatgaca ttgcataatg accctaaaaa gcctgatagc
     8341 aaagaaaaag acaaggaaag aatgatgttt atagaaaata atctaaaaaa agagaatgta
     8401 gactatacaa ggactaatgg tgaatttaaa actgtatttc ccaagaaaag tgattttaat
     8461 gtcagaatta tgaaagaatc taagtactta gaaattacag aaactatagg ggttaagcct
     8521 ataagttttg aagataatga atgtatatca ttattatcat caagtttacc aggaaataag
     8581 aacatcaaag aaaatattat tgtagggaat agaggtttaa aggtggcaaa acaagtggaa
     8641 gaatgtgtta tgccagcata ttacaaaaat atgtatgtgg taaaggataa tttttatgat
     8701 agtatagaat caaaatttat agtggataga tttactgcat ttgaagtaga ggattcaagt
     8761 aaaactatag agatatgcag aaaatttgaa aataaattta ataatgagtc agggatgcaa
     8821 ccatatttgt ttttctctaa agaattaatg ataggaactg ggaaattaat gtattctaca
     8881 tttatatttt tagcaatatt tataggttta attttctttg ttactacaag tagtttttta
     8941 tataataaat tttatatgga ttgccaagtt gataagaaaa agtatgaaca gttaaataaa
     9001 ttgggtatga catataatga gattaaaaaa gcttcaacaa ttgagattgg aatagtattt
     9061 ttgtttcctt atgtagtggc tgttatacat tcaatatttg cacttactgc acttaaaaat
     9121 tcttttgata tagaagtagc atcatcagct gttttggtaa tgggaagctt gttcttggtt
     9181 caaatagttt attttttaat tgtaagaaat aattatttaa aagagataag gttaaattta
     9241 gtcaattttt agttactatt attaaatgat aataaaaagg gtttagaatt aattaatatt
     9301 actggaatta tagatgttta ttttttcagt aatatttttt attgtattaa ctattaaaat
     9361 tttgtacaaa tataaaatat aatatggtaa atgtcaaaat tataaaaaaa ttctacttat
     9421 ataaaatata aagaggttat atgtacatat gtgtagaaat aaataataaa aatatcaatt
     9481 aatgttatta gggggggtta ctcattagta aattagctaa tacacttaga atgatgatat
     9541 taattcaaag cagaggcaaa atgaaatgca aagaattatc agaagagttg gaagtaagtg
     9601 aaaggcaaat aaaaagttac aagatgtatt tagagcaagc tggtatattt ataaattcaa
     9661 cacctgggat ttatggtggt tatgaaatag ataaatgtaa tagcatctca ttaattaaat
     9721 tattagatag tgaagtttca attctagata tgataaattc acagttagag tataataatg
     9781 atatttacaa aaatgaattt aataatattg tagaaaaaat aaaagcagta ttaaatacag
     9841 gagaaaaaag cgatacatat atggattatt ttacagttca agcacaatgt aattgtgatt
     9901 atgagtctga aaaaaataag tgtaatgaaa taattagagc atataccaca aaacacaaat
     9961 tttggataga atattattca ttaaattcag gaaatagtga aagaattgtt catccatatg
    10021 gtttatttaa ttataaatca gatacttata tggtagcttt ttgtgaaaaa agatttaagt
    10081 ttatagactt taaattatgt agaattaaag attataaggt tttagaagaa aaatataatg
    10141 tagataaaag ttttagttgg gatgaatata gtaaaaatag tataggaatt tataagggag
    10201 aagaaattag tgttgttata aaaattagtc atccattttc aacaattata aaggaaaaag
    10261 tttgggttaa taatcaacaa atcatagaat atgatgataa atctataatg tttaaagcta
    10321 aaatgagagg ttatgaggag ataaaaagtt ggattttaag tatgggagct tatgttgagg
    10381 ttgtagaacc agatagactt agaaatgata tattgttaga gattgaaaaa atgaaaaaaa
    10441 tttactgatt ttttcaaata atttatactt ggaccaatat acttcacggt ctagtgttaa
    10501 tatattaatt gagagaggtg atattatgaa aatgaaaatt gaatttagta caggttgtat
    10561 accaatatct tataattcgc tctttatgag tattataaaa gaagcaataa gaaaatctaa
    10621 tgaagattac tataaaaaca tgtactatta taaagaaaag aataataaaa aaactaaaaa
    10681 ttttacattt tcggtttata taaaaaagta tagtattgaa ggtgacaatt ttattataga
    10741 agataaagta atattaaata taagtactcc agatttagaa ttgggttttc atatatacaa
    10801 tgggttaatg acttctaaaa aatgtttata taaagactat gaacttacaa gaattagaat
    10861 cgatttatca agagaaaaga aggtaacaga agaaagggta ctatttaatg cattgtctcc
    10921 aatttgtgta aagtctaaag aaggaaaatt cttggagata actgatgata gatacataga
    10981 agaactaaac tatataacta atgaagtggt taaaaattat agggggaatg gattaaaaag
    11041 aaagttagaa tttgaaaata taggtctcaa aaaggtagta gttaaagaga gtttaagaga
    11101 atttaaaaaa ataacaggta aagagtatca atatattaat ggttacaaag gtaaatttat
    11161 attgaaaggt gatattgatg atttaaatct tatctacaat ctaggaattg gttttagacg
    11221 tgctcaggga tttggagatg tagatatttt agaatggagg tgagataaat gaagttaaga
    11281 gtttatagag gagattggtt ttttaatatg ggtatagttg gatttttaaa tataataaag
    11341 aaagcagata aacaagctga aatatttatt atggaagatt atatcgaatt tgatagctta
    11401 tttcttgaaa actttcatga gtattatttt aattacttta tggatgaata tgatgtatca
    11461 aaaagaatta aaaaaaatat tgattatagt ataaatttta ttaaatctaa gcctgataga
    11521 atcaaagatg gaattaagaa aattaaagat agtgtgaaac aacaaaatga taagattaaa
    11581 aaatttgatg aagaaaaatt taacctgata aaagagaagc tagatagtat gtctaagata
    11641 aaaagttatg atgaatttga cagtttagaa gctttagtag atgagactat agatatattt
    11701 aaaataaaat caataaatga caggcttaca gcaaatcttt ataaatatgt agttcaagat
    11761 aattattttg gacaagttag tttttttaat gttgctaagg cgaaattaga tttagatgga
    11821 ttaaaacagg taatgtttaa tgattattta agacaaatta tatattttgg agagttagaa
    11881 gatttattag aagagaatga ctatgataag ttgaaaaact atttaaatga tagattaaat
    11941 agtatagcta aggatataaa tgaaaaaaaa gttagtaagt ctagtataaa tactatagaa
    12001 aaaattatga aggaaataaa taaaaacttt ataaagaaaa ataaaagcat agaagatata
    12061 aaaaagtata tggatagttt agaagtatgt gaaatgtgtg gtttatataa gggaatatta
    12121 gatgaatact cagaaagtaa ttttgctcca cttggagtaa gcaccaataa tgctaggaat
    12181 atgttttgga atcaggcgta tgtaccatca atatgtgata tttgtaagct aatattgttc
    12241 tgtactccag caggggctac ttatacaaga aagaattatt taattaatga agaaaatgag
    12301 ttttatttat ttgttaacat ggatacttgt ataaatgagc tttttgaaag aaataatagt
    12361 ttaaaagctc aaaaaagtga gtattcagat tccaaagagg aaaatccttt taatcagctt
    12421 ataaaaagta tagtagaaga aaatacattg aaaagtgaat ggcaacttcg taatatactt
    12481 tttgtggaat ttaaagcaag tatagattct aaaaaatgta aaataaatta ttttaatatt
    12541 ccaacttatt tagcaaaatt ttttacagat aagtatgcaa ataaaaaaat acaaagtatc
    12601 tatgattaca agcttaaatc aaatgtacta gatttactct tgaaaaatag ggacttgaag
    12661 catctaatta ataacatttt aagaaataaa gttaaaaatg atatggaaag caacaacaaa
    12721 tcaaatatat caggaataga ttgttttaga gtagtccaat taagagcatt aataaatagc
    12781 tacaaaaagg gggtatataa aatggatagt aaaaatttgg agaagaatga tgaaaagctg
    12841 agaataattt attatttagg ttgtgatata catgactatt ttgttaataa aaattctaaa
    12901 aataaaatag gtggagtaag ctataaactt ttaaattcag ttaaagtcgg aaataaaagt
    12961 gattttatgg atacaattat aagagttttt atgtcagctg aaaaacagat gccagcattt
    13021 attttagata tagaaattga aaaagattta gactttgaat ctataggtca tgcttttata
    13081 tctggattaa tttctgggaa atatgaagga aaagataaac taccaaacga aaaggaggaa
    13141 aaataagatg aaaagagggt taacatttac agtaatagca caagctcaaa gtttaaacta
    13201 tggtgaaggg ataggtaata tatcagaatt aaaaaaatta agtagagatg atggaaatat
    13261 atatacattt gcatctcgcc agtgtttaag atatgacata gtacgattag cagcagaatt
    13321 gtttaattgg aatttacagg ttgtcgataa agaaaaaggt actattcaat ttaaagataa
    13381 tataagtata agagaatcag aagaaatgga tttatttgga tatatgaaaa caaataaaaa
    13441 agatgagaaa gataaaaaag gaggttcttt gactagagaa gctactgtaa gattaagtaa
    13501 tgcgatttct cttgagccat atagaagtga tatggatttt ttaaataata aaggatttgc
    13561 agatagaata ggagaacacc ctaatcttgc taacatagag caacatttaa gttactatac
    13621 atatacagtg actatagatt tatctaaaat aggcaaggat ggagagatag aacttgataa
    13681 taaagagaaa tgtagaagag tagtagaatt tttagaaata ataaaagtat tgaatagaaa
    13741 tattagaggt agacaagaaa atttatctcc tctatttgta gtaggaggag tatatgaaat
    13801 agcaaatcca tttttcttgg gtagaattaa attaaaagga gataaaaatg gatttaaaat
    13861 aaataaacaa gctatagaag aagtaatcca aggtactttt ttaggaaaag atttaaaaga
    13921 atttacctat gttggaatgg tagatggggt atttataaat aaagaagaat ttaagggatt
    13981 gtttgaagat aattttttta gtgtagataa attttttgga caactagtta aagaagtaaa
    14041 ggaatattat ggggtgaatt aatatgaagg ttttgaaatt agatttattt caagaaactg
    14101 catgctacaa aaaaccattt gctatgaaga ttacagagac atatccactg cctccatatt
    14161 ctacaataaa tggattaatt cacaaaatat taaatgctac agaatatata ccatttaata
    14221 taagtgtaca gggaacatat gaaagtatat ttaataatta ccagacaaca tatttttata
    14281 aaaaagatag tattacatct atgccaatga atagtcatat gctattgaat gtaaatttga
    14341 taattcatat aggtggggat tttgatttat tggaagaaat atatgatagt ttattaaatt
    14401 atgacgagca cttatcatta ggtagaaaag aagatttagt aagaattaat gatattagat
    14461 ttgtagaagt caataaattt gaagtagatg aggatataca attagatgat tatgaaaatt
    14521 ataagttatc agaatgtaat ataagaatgc ctatatatat accaaaaaca agtttacaag
    14581 atacagatat agatggtata agttacagac tcaataatta ctatgataat aatgctgtgg
    14641 aagataaaaa aagggtttgg aataaggtag atagttatta tgttgaggat ggaaatacta
    14701 tatcaattgg agatatatta ttagataatg aaggagattt attatatttt aataatataa
    14761 attaggaggg agattatgtt atatgctaaa tctaatccag tggaaacact tagagaacat
    14821 actgatgaac ttttaaaaca aatgaatgta ttaagagaaa gttatggaaa aaatataaat
    14881 agcttagatt ttctagaaga ggagatattt tggcaattac ttgaccttgt tatagaattt
    14941 catgatattg gaaaggcatt ttcaccgttt caagagttaa taaaaagcag aatggatact
    15001 aaaataaatg aacctaaaat agtaactcat ttagaaaata atgtaggcca taattatctt
    15061 tctcctgcat ttattgatta tagttatata gacaggaaaa aaaataaaga acttagagca
    15121 gtattaaatc aagttattgt atatcatcat gaaagagata tctttataga taaagatttt
    15181 aaaaatttaa tacaaaaaat attagatgaa gatttaataa ataaagttta tgaactacag
    15241 catgaattta aagttagata tccaataaaa acagaaagat tgagtaaagt atatctacaa
    15301 agtgtagaaa aaagaataga taaaaatcac aagtactata atttatatat tatgttaaaa
    15361 ggaatactac atagactaga ccatagtgct tctgctcatg aagttgtgga atgcaataat
    15421 gttataaata taggagaaca gacagaaaat tatttaatga aagagtttgg tagtttgaga
    15481 gaggctcaat catttgctaa atctaataga aataagaata taatactgat tgcatctaca
    15541 ggaatgggaa aaacagaaac tgccttaatt tggatagata aagacaaggc attttttaca
    15601 cttccattaa gagtaagtat aaatgcatta tttgatagag caaaaaatat agttggagtt
    15661 ggagaatcaa atgatacatt cttaggtttg ttacattcaa cagcaataga ttatctagaa
    15721 gaaagtaatc aagaaaattc aagtgagatt gtagatttag caaagttatt atcttgcaaa
    15781 ctgacatttt ctacaataga ccaaatattt aaatttcctt ttttgtatag agggtatgaa
    15841 aaagtgtact caacattagc atattcaaaa gtagtaatag atgagataca agcatattca
    15901 ccagaaatag cagctgtatt ggtaaaaggt atagaaatga tacataagat aggtggcaga
    15961 tttatgataa tgacagctac tatgccaacc atatatatag atgaattaaa aaaaagaggt
    16021 gtaatgaata gtaacttagc tgaattgaca tgtaatacag aaaaaataag acattgtgtt
    16081 tctattgtag aaaattcaat agatgaaaac ttaggtaaaa taatacaaag tggaatgaat
    16141 aaaaaagttc ttgtaatagt aaatacagtt aaaagtgctg tagaaaaata tgagttaata
    16201 gaagaaatag ttaaaccaaa aggaatagat gtcaacttaa atcttttaca ttctatgtat
    16261 atacaagaag atagagcaaa attagaaaaa tatataaaag aatttgccaa tagtgataga
    16321 aatggaattt ggattacaac acaattagta gaggcatctc ttgatattga ttttgatgag
    16381 cttcatacag aaaattcaac acttgacagt ctatttcaac gatttggtag atgttataga
    16441 agtcgtgagt atgaagaaaa tagcccaaat atatttatat atacagaaaa agctacagga
    16501 ataggtagta tttatgataa ggatattgta gaaaaaggac ttgagctact gaaaactttt
    16561 ataaatggaa aagaatgtgt gaagatgaaa gaaaaatata aggtagaaat ggttaagata
    16621 ctatactcta aagaaagtct agaaggtaca gcttttgaaa agagatttac cagtgcttta
    16681 aatattttag atactataac gccatatagt ttaggttcta aagatgcaca aaatatactt
    16741 agaaatattg aaggatatac agtaattcca gaggatatat ataataaaat tgaagaaact
    16801 ttaatcaaag attatgaaga gttgggacaa gagcttataa atgcatatag taatgatgat
    16861 agacaattga taaataaaat aaagtctaag agaaaaaaag caagaagaga gataattaaa
    16921 aaaactgtaa atttaccaat atataaagct aaacaaaatg taattgacat acaggtgaaa
    16981 ggtcttgaag atttaaaaat acttttatat aaatatgata tatacgaaaa taagcataca
    17041 aaacagatat ttggtaaagg tgtgcttata agtaatgaaa tagaccaatt tatttgattt
    17101 ttatattaac aatgtggcta aaatttacta aagaaggtga ttttgtgcca atagatttag
    17161 aagtattaaa agttcaaggt gtaaaaataa attattatta tgtatgtaaa agaaaactat
    17221 ggctattttc taaaggaata accatggaac aaaatagtag tagagtattg tctgggaaga
    17281 tagtacatga agattcatat aaaagaatga aaaataaaga aatattgatt gatgatatct
    17341 tgaagctaga tataataaag gatgagtatg taaaagaagt aaaaataagt tcaaagatga
    17401 aagaagcaga tgaaatgcag ttatactatt acttatacta tttaaagaat agtatgggta
    17461 tagagaaaaa aggaactata aattatgtta aggaaaagaa acaagaagaa ttgatattga
    17521 cagaagaaaa agaaagaatt attgaaaaca cacttataaa tataaatgaa ataaatatgc
    17581 agttatctcc ttccaaaatt gagaaaaaac attattgtac aaaatgtgct tattatgaat
    17641 tttgttttgt gaaggaggag taacaatgta taaagactat tatattataa gtaatgggga
    17701 tataaaaagg aaagaaaata cgatttacta ttacaattta gaaggtgaga aaaaaatatt
    17761 accagtagaa caagttgatg atattcatat atatggagaa gtagatttaa acacaaaact
    17821 tataaattac ataagtaaat atggggtgat gcttcatttc tacaattact atggatatta
    17881 ttcaggtaca tattatccaa ggaagaaaaa tgtatcagga ttcagtttag tatgtcaatc
    17941 agcctgctat ttagataaaa atgaaagatt atacttagca aaatcctttg tggaaagtgc
    18001 atcataccat attttaagaa atatgagata ttacaaagtt gatgaaagtt taattgatac
    18061 tataaaggat gaactaaatg atttgaatga tgtcaaagaa atatcagaac ttatgggtaa
    18121 agaaggcaga ataagaaaaa catactataa atcatttaat caaatattaa aagatgattt
    18181 tgaattttat aaaagagaaa aaagacctcc caaagaccca gtaaatgctc ttatgtcttt
    18241 tggaaatagc ataatgtata cgaatgtttt aagtgagata tataagacac aacttgaccc
    18301 tacaataagc tttttacatg aaccatcaac aaagagattt tctttaagtt tagatattgc
    18361 agaaattttt aagccaatta tagttgacac aataattttt agcctgatta ataataaaag
    18421 aattaataag aatgattttg aatatcaaga caaaatttgt tatttaagtg aaaaaggaaa
    18481 gaagaaattt ttgcaagaat ttgaaaataa gatgcagaca actataaaac atagaaatct
    18541 aaatagaaaa gtttcatata gaatgtttat aaggttagag tgttataaat taattaaaca
    18601 ttttataaat gatgaaaggt acaaagtttt aaaggtgtgg tggtaagtat gtttgttatt
    18661 gttacttatg atattgttga agcaaggtcg ttaaatagaa ttagaaggat acttagaaaa
    18721 tatttgactt ggacacaaaa ttctgttttt gaaggcaata ttactgaagg aaagttacat
    18781 aaatgtattt ctgaaataga aaatattatt gataatagcg aggattcaat ctatgtttat
    18841 gagataaaaa atcctaattc aattaaaaag aaatgttatg ggattgataa gtattctgat
    18901 gaaatgttta tataggtttg cagtgagcga tatttatgct aaaataggtg ttaacagttg
    18961 gaatataagg gattgaaggt gtatgataac tgttatcaat tgcactactg ctcgctcact
    19021 gcaaattttg atgtttttat tgaattataa ttgcttgatt gaagtgtttt caatgtattc
    19081 aaatatacct attttggggt tttatattaa ctaagtggta tgtaaattgg aaaacttgtc
    19141 aaaaatagca tcaaagcatt tatagtttta tattaactaa gtggtatgta aatggggctt
    19201 ttggggacat gagtgttaca gatgcactaa gttttatatt aactaagtgg tatgtaaata
    19261 gccccctaat gctggaacag tgaggacaca caaacgtttt atattaacta agtggtatgt
    19321 aaatgaactt ttaaaagatt atgctaggaa aattcatgtt gctggtttta tattaactaa
    19381 gtggtatgta aataagccaa aagctacgaa tttgaactaa aatgcgtggg ttttatatta
    19441 actaagtggt atgtaaattg cctagggaac ccctccagcc cctaaccatg caacagtttt
    19501 atattaacta agtggtatgt aaataacaag aaaaaccaac aacaaaacca acaacaaaaa
    19561 ggttttatat taactaagtg gtatgtaaat ctttccataa aataccccct acttcatttt
    19621 tatttagttt tatattaact aagtggtatg taaataagac caagccactt ccgcaattgc
    19681 atcttctaac atgttttata ttaactaagt ggtatgtaaa tagtgtattt ttatcattct
    19741 atctactata ccaaaaaagt tttatattaa ctaagtggta tgtaaatgca tacctgtaac
    19801 ctgcatcatt ttgtatctat cttgttttat attaactaag tggtatgtaa ataaaagatt
    19861 gtaatgagta cgagacaaga tttattgtgt tttatattaa ctaagtggta tgtaaattat
    19921 ataaattttc tttttcttct atttctaagt tcagttttat attaactaag tggtatgtaa
    19981 atgttgtata gctagtactt cctgctttaa atccttttcg ttttatatta actaagtggt
    20041 atgtaaataa gacaaggttc ttcttttaag tggcaagcta taaaagtttt atattaacta
    20101 agtggtatgt aaatcgagta aaataacaat tatctggaat gtgagaagtt gttttatatt
    20161 aactaagtgg tatgtaaatt acacataatt acaacccagt ttactttttg ctacacgttt
    20221 tatattaact aagtggtatg taaattatga atatattaca taatcataaa gatttacaaa
    20281 tggttttata ttaactaagt ggtatgtaaa ttctctaaat ccatctataa cgttaccagc
    20341 aaaatcaatt ttatattaac taagtggtat gtaaataaga acttttcatg aacgtggaaa
    20401 atattgagtt cagttttata ttaactaagt ggtatgtaaa tttgtcattt acgactacca
    20461 tttctctagc atttatatgt tttatattaa ctaagtggta tgtaaatggg aaaatatggg
    20521 aaaagttaac taatataatg caaagtttta tattaactaa gtggtatgta aatgaaattt
    20581 ttagtgctat tgcaactact cttgtttgtg ttttatatta actaagtggt atgtaaatgc
    20641 ataaccaaaa acttcaaaat caagacctcg aatttgtttt atattaacta agtggtatgt
    20701 aaattgcgca ataacatgct gaaaactgca atttcgaagt gttttatatt aactaagtgg
    20761 tatgtaaata ggataaagaa aagactcaca caaggcacag tgtcaggttt tatatcaact
    20821 aagggtagat aagtagaata ttagcaatat caacgagtat ttagaaactt attttatatt
    20881 aataaagcag tatataaaat tgtagaatca acaatagcat atactaaaac atccacctta
    20941 tattaactaa atcatatcta aataaaatac acttacctat aaacattata aaatcaatac
    21001 aaaaatgagg tgaaataaaa tttatgataa agaaattaaa caataaagac ataaataaaa
    21061 tcatggaaat atgggaaaaa agtacaatca aagcacatga ctttataagt aaagaatact
    21121 ggcaaaataa ctataatact gttaaaaacg aatatatacc tatatcagat acatttgtat
    21181 atgatgatgg agatgaaata aaaggattta taagcataat agataaaagc tttataggag
    21241 ctttatttat aaagcccaaa taccaaaatc taggtatcgg aggtaaactt ttagattatg
    21301 caactaaaaa atataaaagt ctaagcttag cagtatataa agataataaa aaagcagttg
    21361 tgttttataa taaaaaaggt tttaatatag taaaagaaca agtaaatgaa gattcaggat
    21421 ttaaagagta cataatggaa tatagtaaat aatatgatta cattctatta aaaggataat
    21481 taatttattt gccatttgat tttttaagag taatttttaa aatagactat atgaagataa
    21541 agatatttat tatagatgta agttaatcag aattgatata gctacaaaag aggaaaataa
    21601 aattgtatgg tgtatttcag atttaacaa
//
LOCUS       DAELNY010000050        21424 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.050, whole genome shotgun sequence.
ACCESSION   DAELNY010000050 DAELNY010000000
VERSION     DAELNY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.050"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(221..1186)
                     /locus_tag="KPE55_001627"
     CDS             complement(221..1186)
                     /locus_tag="KPE55_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492716.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEITVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEINIMKEDMSEVRQ
                     EINTMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEM
                     TVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNG
                     IKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIE
                     HNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1416..2501)
                     /locus_tag="KPE55_001628"
     CDS             complement(1416..2501)
                     /locus_tag="KPE55_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0492717.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2537..3850)
                     /locus_tag="KPE55_001629"
     CDS             complement(2537..3850)
                     /locus_tag="KPE55_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0492718.1"
                     /translation="MIAIKSKYLISSADEIYANSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNSP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETILASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3851..6142)
                     /locus_tag="KPE55_001630"
     CDS             complement(3851..6142)
                     /locus_tag="KPE55_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0492719.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6132..6926)
                     /locus_tag="KPE55_001631"
     CDS             complement(6132..6926)
                     /locus_tag="KPE55_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0492720.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(6965..7411)
                     /locus_tag="KPE55_001632"
     CDS             complement(6965..7411)
                     /locus_tag="KPE55_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0492721.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7483..8889)
                     /locus_tag="KPE55_001633"
     CDS             complement(7483..8889)
                     /locus_tag="KPE55_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0492722.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(8907..10277)
                     /gene="hydA"
                     /locus_tag="KPE55_001634"
     CDS             complement(8907..10277)
                     /gene="hydA"
                     /locus_tag="KPE55_001634"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0492723.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGEDLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10410..11597)
                     /locus_tag="KPE55_001635"
     CDS             complement(10410..11597)
                     /locus_tag="KPE55_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG0492724.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11724..12938)
                     /gene="dpaL"
                     /locus_tag="KPE55_001636"
     CDS             complement(11724..12938)
                     /gene="dpaL"
                     /locus_tag="KPE55_001636"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0492725.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13301..13972)
                     /locus_tag="KPE55_001637"
     CDS             complement(13301..13972)
                     /locus_tag="KPE55_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0492726.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14055..16271)
                     /locus_tag="KPE55_001638"
     CDS             complement(14055..16271)
                     /locus_tag="KPE55_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0492727.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16274..16744)
                     /locus_tag="KPE55_001639"
     CDS             complement(16274..16744)
                     /locus_tag="KPE55_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG0492728.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(16806..18200)
                     /locus_tag="KPE55_001640"
     CDS             complement(16806..18200)
                     /locus_tag="KPE55_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0492729.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18273..19634)
                     /locus_tag="KPE55_001641"
     CDS             complement(18273..19634)
                     /locus_tag="KPE55_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0492730.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(19931..21277)
                     /locus_tag="KPE55_001642"
     CDS             complement(19931..21277)
                     /locus_tag="KPE55_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0492731.1"
                     /translation="MSKYQKNVSMFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVV
                     GGAVGVSPTQITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVG
                     GNYGLASILGAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMA
                     GGVNSPTYASMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIN
                     FDPIREATWFAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNIT
                     DKELSSGVIGSGICTMIGALFGGLPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGF
                     VPKFGALMTTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGI
                     TSVPAAQEAFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
BASE COUNT         6362 a         4363 c         2383 g         8316 t
ORIGIN      
        1 tatttcaagc gttctattag attttatcat ctaaatttta ggttttattt ttataaatta
       61 attgtattta cataaaaaaa aagacctaaa acaaattttt aacggtcttt tctcaatttt
      121 ttattttcat ttctaaaatt tataataaat agtaagaaca ctataaattc ttttatataa
      181 aattcttagt ttgtggatga taaattagtg aattaaaata ctactttact gattttagtt
      241 tgacaatatc actccaattt ttactagtgg ctaactctac taaatttaag tctcttctta
      301 tttcagctat atcctctttt atagaattag tgtcaccttt tatatatgct atgttatgtt
      361 ctatattatc atgagtagct ttatttattt cagaattatg taataaactt cctaaaatat
      421 tattctgttc tatctgtatc tctttcattg atttatcttc acattctaga ctatcaatct
      481 taatttcaat tccatataat ttttcatttg ctatggatac ctcgtcctta attccattca
      541 tatcttcatt aatgttattt attttatttt gcataaagtt tacttctgat ttaacttcgg
      601 aaacatcttc tttcattaca gttatttctt gtctcacttc tgacatgtct tttttcatta
      661 catttatctc ttgttttacc tctgatgtat cttctttcat tacggtcatt tcttgtctta
      721 cttcggacat atcttctttc attatattta tctcttgtct tacttcggac atatcttctt
      781 ttaatacatt tatctcctgt tttacttccg acatatcttc tttcattaca gtcatttctt
      841 gtcttacttc cgacatatcc tctttcattg tatttatctc ttgtcttacc tcggacatat
      901 cctctttcat tatatttatc tcttgtctta cctcggacat atcttctttc attacaatca
      961 tttcctgttt tacttctgac atatttcctt ttaatacatt tatttcctgt tttacttccg
     1021 acatatcttc tttcattaca gttatttctt gttttacttc cgacatatct tcttttaaca
     1081 catttatttc ttgttttact tccgacatat cattttttat gctaactaca tcttcattta
     1141 tagtatataa taactctaaa atcttctctt gcacttgcat caccatccct attaattatt
     1201 ataacatttt acacaaatgc tatacaatgt taacatatat atcagaatta ttaaaatttt
     1261 aattaaacaa aattgattca ctttattact tcaaaactaa ttaatatcta aaataactac
     1321 aaataaacaa aaataaggtc aacaacaaaa aaggcttatc tctttgagat agcctttctt
     1381 atttttattt aaaactttgt tttttataaa ataatctata cattcagctc tgaacatgaa
     1441 atttttcttc caagtgcttt tggtttacaa actgatatac aaagtccaca atatctacac
     1501 tcttcttcat tcaattccat cttatcattc aattttctcg cttgatatgc acaaactctc
     1561 tcgcacatct tacatttagt acatttttct tcatcataag taaactcaaa gctaagtctt
     1621 tcattaacat ctcccatatg attatgtact ataccacaag cattttctat agtattatat
     1681 cctaatctat taagattaga ttttaaatca tcatgtattt tagatattac ttgtgcgcct
     1741 tttaaaatta ctactgaaca tataccaaca aagtcagcac ctgccataat catttctaag
     1801 gcatcatctc cacttacaca tccacctaag ccaattattg gtatattaac ttgttttttt
     1861 atttcatata ttctctgtaa agttattggc ttgatagttt ctccacttaa atatcctatt
     1921 ccatttccac ctaataaagg ttgccctgtt tctatgtcta ttctaaatgc tggtcctaca
     1981 gagtcacaag cagttattgc atctgctcct gcttcttcta attttttagc aaaatcgcct
     2041 atctcatcta tcataggagg aagtttaact attacaggta tatttactct ttcctttgca
     2101 tctttaagca ttggtatcaa atctctatag tcataagata ctaattcaat aaagtcagct
     2161 cctgcatttg caactttctc tactaattcg ctactttctt caattgtatg acctattgaa
     2221 gctatacaga cagtcccatc tcttttcatt tgtggtattt caaaatctat ccattgctct
     2281 ggctcaaaat ctgtccattt ttcattattt aaaagagcat tagctgtatt tctaaccata
     2341 tgtggtgctg gatttatagc tctttctttt cttatagtct tagtcacaac tgcccctgct
     2401 cctgcatcac ttaatatctt gcatccagct gcactatatg ttaatggtcc tgaacctatt
     2461 ataagtggtg attttaattc tttacctaaa aaattataca ttatctgctt ccccttttca
     2521 tatatattta tattcgttaa aatttccaaa attcacaagc aacttctttc atttctttaa
     2581 caatagcttt tttatctaaa tcaactaact gtttttcttt taaaatatgt ctaccatcta
     2641 tgtatacatt tgatataaat tcttttttac cctgaactac tatctggtca aatatattat
     2701 caagagtcac aggagtttta aattcatctt ccataactat tatatcagcc ttattaccaa
     2761 ctgctatttt accagaattt tgaagtccta atacataagc accatgctct gttgccattc
     2821 taaatactaa atttgctggc attactgatg catcttcttc aactgattta tgcattaaaa
     2881 atgcacctct cataacttca aaaaaatcat ttatatatcc atcagtaccc agtgccactt
     2941 ttatgccttt tttaatcatt tttggtactg gagaaaaacc tccacctact tcacagttgc
     3001 taagaggcat atgaactact tttacacctt tttcttttaa tatatctatt tcttcatcat
     3061 ttacttttac acactgagat gctaatatag tttcatccaa tatatctaaa tcattatagt
     3121 agtcaactgc ttttttgcca aaatgttttt cagcatagtt tggctcatat atactctcac
     3181 ataaatgaaa ttgcattggt gaatttaatt tttttgcatc ttctttagct ttcttaataa
     3241 atctatcaga acatgtgaag cttgtatgtg tacacattat tccatttatt aatttactat
     3301 tttctctact ccactttatt aaattagaat tttcatctaa acatctaagt ccattttcaa
     3361 agcttattct ttcacaactt tctaaggata aaatcgcttt catgcctatt tcttctagta
     3421 tttttccttg ctcaattaaa gttccttctt ctgtatttgg agcttctagg gtatcacaaa
     3481 aagctaccac accagattct ataagttcta ttgctgatgc tttagttgtt gccttcactt
     3541 ctttcaatcc aattctattt tctataaatg gccaccaata gtcatttaaa aaaccttcaa
     3601 agtcagtaaa atgtacattt gccggtattc ctctagaaag aactccatat tgatgcatat
     3661 gaccattgac gaatcctggc attattatag catctgattt atctattatt tcttcaatat
     3721 ttttatattt tatttcaact tcttcatttg gcaatatatc ttttataatg tcattttcta
     3781 ctacaacagc agaatttgca tatatttcat cagcagaaga tattaagtat ttacttttaa
     3841 tagcaatcat ttatttacct ccctgattta attttttcat ggccatataa actttctcag
     3901 aagttatagg aagttctctt atacgaactc ctgtggcatt gtatattgca tttgctattg
     3961 ctggtgcagg tgtatgaata actatctctc ctaaagattt agctccatat ggacctgttg
     4021 gctcataatt atctacaaaa tcaacaatta tgtttcctat atcttttttt gttggaactt
     4081 tgtattgtaa tagattagaa gtttgaagct ttccatcaga accatgtcta gggtcttcat
     4141 acattgcaag ccctatacct tgagttacac caccttcaac ttgtattcta gcaagtgcag
     4201 gattcataac tgtaccacaa tctgtagaac aagcataatt tattacttta aactcccctg
     4261 ttgttttatc taactctatt tcagcaaatc ctgctaagaa tggtttagca ctctcttgta
     4321 taccataaga ttcagatgaa tataaaactt cttgatttcc accaccaaca gattcttttc
     4381 ctaattcact taacaaaaca aacttctcta catcttcact ataacaaact ctgtcttcat
     4441 atagtattaa atcatctatt ggtaaatcga gtttcttctg tgctactttt aatatctttt
     4501 gttttaaact ttcagcagat tttattgtag catttccagt tacataagta gtacaagaag
     4561 cgtatgcacc agtatcatat ggacacatat ctgtatctcc tgtataaacg gaaattctat
     4621 caatagatgt attcaatgct tttgcagcta tttgtgcaag tattgtgtca gaaccagttc
     4681 ctaagtctga agacccagaa aacaacttat aagttccatc ttcatccatc ctcatattta
     4741 ctattgccat gtctatgttg gctataccag aaccatgagt tcccacagac attccaacag
     4801 ccctcacctt attgttgcca atatccctaa cagggtactt ttcatcccat ccaataagtt
     4861 ttttacctct aattatacat tcatcaagtg cacaacttct tattggaaaa ttcattattc
     4921 cgccttcagt ttgttttttt ataatattct ttaactttat ttctgttggg tccatattaa
     4981 gtttatgcgc taattcatca attgcacaat caagtgcaaa tgtaccttgt gttgctccat
     5041 atcctcttaa tgctcctcct ggaactaaat tagtatatac agttctccca tcaaatttta
     5101 ttgcaggtac attattatat gttggaagta cattatgacc tgattcagag caaactgaag
     5161 gtccattatc accataagcg cctgtattgt tcaatgcttt catatctata gcttttatgt
     5221 ttccttcttt atctgcccca atttttatat caaaaaacat ctgatgtcta gtattagtca
     5281 ttgcaaaagt ttcttctcta gtataaatta gttttgctgg tctttttgtc atccaagtta
     5341 caaatgatgc atatatctct gttacagcta tgttttttcc accaaaacct ccacctattc
     5401 ttggttttat tactctcact tttgaaagag gtattcccag tgctttagaa acctgtcttc
     5461 tcatatgata agctgattgg tttgcagaag ttactactaa tctgccattc gtatctatat
     5521 acgtataagc tctatgagtt tccatcatac aatgggcttg tgattgagtc atataagaat
     5581 gttctactat tacatcactt tctttaaact ctttttctac atccccttta cccatttttt
     5641 ctctcgatac aatatttttt gtagcatcca atccaaaatc aaatggacaa aacaaatcat
     5701 cttcttcatg gattaaaatt ttatttcctt ccgattctct tgcatccaat ataggagtca
     5761 ttacttcata ttcaactttt ataagtttca tagctttaat agcagccctt tcatcttcag
     5821 cggctattat agcaacttca tctcctacat atctaactac gtcttcaagt attaattggt
     5881 catgaggtga tgcttctgga tatgactctc caaccattga atatctataa tttggaacat
     5941 ctttgtaagt gaatatgttt actacacctt ttacctttaa tgcatttttt atatctatgt
     6001 tttttatctt cgcaaaagca tgtggacttc taagaagctt aattgttaaa acatctttat
     6061 gaaaaattaa atcttctgta tatattggtt ttccagtaac aatggcttca ctatctattt
     6121 ttcttacctc tttatttacc atcacactca cctccaatac tattatacat tctcactact
     6181 aaggcttttg ccatatcttt tctgtattct ttacttcctc ttatattact tccaaatgaa
     6241 agttcttcac ttgatacttc accagctttt tctatatcat tttctaaact taatatttca
     6301 cttgctttat aggcaatttt tgctcttctt ggtctagcgc ctattgcaat tttataaata
     6361 cttccttctt ttaacattgc tccatttaat actgcaaaat cacctgtaca ttttcttata
     6421 gaatcaaata cacctatagc atctttttta ggtaatataa cttctattaa tatatctttt
     6481 tcaagctcac tttctaaaaa atcagctaag ttcataaccc cttttttata taacttaacc
     6541 tttgcatcta ccactagtaa tgaaggtatt aaatctgaaa atccatactt agcaaatacg
     6601 ctagctccta ctctagctcc acttctaaat tgaactccta ctatattaga aacagcgttt
     6661 gaaattaccc cattacagta atttttaata accttattta attctaaagt tcttaaactt
     6721 gtgtctgctc caattaatat gctatcatca gtttcattta tataatctaa agctaagtct
     6781 tttaaatcta ttgctgatcc aatattttta ctacctagtt ttaaaaatcc acatcctcca
     6841 agtattatgt tgttttttct tgatattagc agttcataag cttcatctaa actttttgga
     6901 actacatatt cctttatagt tatcatactc tctccccata tcaacttaat tttcacttaa
     6961 aagtctaagt gtttaaatat ttatgtattg ctcttaattg acctttatat cctgtacatc
     7021 tacaaaggtt accatttaca taatctataa cttcttcttc tgttggattt tgaagctctc
     7081 ttttcattgc caaaaccatc attataaatc caggagcaca aaatccacac tggtcagccc
     7141 cttcgtctgc aagaaaatct ccaaacttct ttgcttcatc caagacaccc tcaagtgttg
     7201 taatatgctg accgtctact ctagcagtaa gataattaca tgaaagcacg gccttgtcat
     7261 ttaccatcac tgtacataac ccacatgagc ctgtatcaca ccctttttta acacttacat
     7321 atccattttc tcttaatgta tctgctaaaa aatcatctgg tcttatactt aattcttttt
     7381 ctttattatt tatggtacat ttgataatca aataaatcac ctcaaaactc atatttttaa
     7441 gctaaagttt tactatactt ttctatgaat aaattacatt tattaataaa ggctattcca
     7501 tttatgacct tccattctat tttttatagt tcctcttaca caaagtctct cagctgtatc
     7561 ttgagcatct tttaatgctt tctcttcatt agctgtcaat atttttgaat cttccattat
     7621 tatatttcca tctactataa cactctctat atttttcata ctagaagaat atactaatgt
     7681 agatactgga ttatgcatag gtatcgcttt tggtgaaagc attggattaa atattagtaa
     7741 gtctgctttt ttacctatct ccaatgaacc tatctcatct tccattccaa tagctcttgc
     7801 tccatctata gttgccaact caagaacttt ttctgctgac attgctaatg ggtcacactt
     7861 attaacttta tgttgaagtg ctgttaattt cataagttca agcatatctt gagagttatt
     7921 actagcggct ccatctactc ctaaactaac tgttattcct tttttaagca tttctggcac
     7981 tggtgcaaca cctgaagata agtacatatt acttgctgta ttatgagaca ctttcatatc
     8041 gtatttttta gtaagttcca tatctttttc agttagatac acacaatgta ccataagcac
     8101 atttggtcca agtattccta atttttctaa aacatctatg tcatattgcc catgcaattc
     8161 ttttgctgct tctctatcaa aaggagtctc tgatatatgt actgtaaata aagcatcatc
     8221 atattctttt actactctcc atagcatttc aagcatttct tgagaatttg accatattgc
     8281 tgcaggagct actcctattt tgattcttcc attttctgta ttatggtgtt tttcaaacaa
     8341 tcttcttaca tctttttcaa ccgtttctac atcttgcatt attccaggat gaactccaaa
     8401 ttgagctcct gtattcatac atcctcttcc aagtattcct cttattccta attctttata
     8461 agcatctatt attccatcac aaagacctgg cttactatga ggatacatgt agtcaaccat
     8521 tgttgttata ccacttctaa gtccttcaat acatccaagc attgcagcat cataagtatc
     8581 tttgggctct aaatagttag ctgctggaaa tgtcatagtt tctaaccagt ctttaagtac
     8641 catatcatct cccaatcctt ttaataaagt ttggaataaa tgattatgtg tatttataaa
     8701 acctgggaat aaaactttac ctttagcatc tataattttt ttaacatcag tatatttact
     8761 ttctatctct ttagaatttc caatatcagc aatcttgtta tcttttacta ctaatgcccc
     8821 atcaaaaata acttctctct ctttattcac agttactatt atggcattct taataagtat
     8881 atccatcatt cttatttccc cccgcattat ttagtaattt ttcttttaat atattgacct
     8941 gttggatgtt ctttaacatt acaactttgt actacatgat ttcctctcaa aaatacatct
     9001 tcaactttac atttaacctt aaatccttca tatggagtat aatctacatt ttgtttttga
     9061 gttttataac taattgtttc ttctttattg atatttaaaa tgactatatc tgcatcactt
     9121 cctacagcta tctcaccttt tttaggatac attccaaata tttttgcagc attcgtagaa
     9181 gtaacttcca caaacttatt tacacttatt ctattttcta agaccccata tgtatataaa
     9241 agtgctaacc tatgttcaac accaggtgct ccatttggaa tcttactaaa atcatcaatt
     9301 cccaagtctt tttgcccctt aaaattaaat gaacagtggt ctgtgccaac tgtttgaata
     9361 tctccactag acaatccttc ccaaaggtaa tttatgtctt ctttttttct taaaggagga
     9421 gacattacat acttagcacc ctcaaattta tccttattat ataaattgtc ttctaataat
     9481 agatactgtg ggcatgtttc tactatcata tctatatttt tttctctagc catttttaca
     9541 gtttcaagtg actccctaca acttaagtgg actacatagg attttgaatt agataattta
     9601 gttatatcag caagtcttga aacagactct ttctcaacta tgttaggtct tgtaagtgga
     9661 tgatattttg gctctaaatt accattttgt atattttctt ctatcaatac atctagtaaa
     9721 tccccatttt cacaatgaaa tccaactata caacctaaat cccttgcttt ctttaaaacc
     9781 ctgtatattg ttccatcatc tactttcatg ccatcatatg ccatatacat cttaaaagat
     9841 actatcccat ccgaaataag tttttccata tgattaaatg tatctttatt taaacatgta
     9901 attgtcatat gaaaaccata atcacaataa caatttccac ttgctttttt atgatatgct
     9961 tctacacctt gcagtaggtc ttccccctca ttggcttctg caaaatctag tattgtagta
    10021 gttcctccag ctatagctgc tcttgttcct gtttcaaaat tatctgcagt tgtaatagaa
    10081 cctgcattca tatcaaaatg agtatgtgtg tctattccac caggaattac aattttacct
    10141 gtagcatcaa taaccttatc accatttatt tctaagttat ttcctatttc aactattttt
    10201 tcatcttcta tccttaaatc acttatataa gtcttatcag aggtaattat agttccacct
    10261 cttagtattg ttcccatata tacctcctaa cttttaagct ttaaagtatt ttctaagtat
    10321 tgtaaaactt tatttaaaat tgtattttct aaaggtgcta tattcagcac ctctagacta
    10381 tatttaaata atttattgtg aactaaatat tattttccgt atacagattt taatattcca
    10441 tagtaacctt ctgttcctat aaataactgt tcttgctcta tatactcatc tattgtgtga
    10501 gcaagatttt ccttagaagg accaaatcct atagttttta ttccagcttc acctgcatag
    10561 tgacttccat tcgtacaaaa tgagtattga gttatttctg aatctattcc agcttctttt
    10621 aatcccttat atgctgcttg aacaaattca tcttcttcgt catataacca acctgggaag
    10681 aatctttcac tttctatagt atttcctgta tagcaatcag ctttttcaac cgcataagaa
    10741 acttttgcat ttaattctgg gtcttctttc atcatttcat ctaataaagc ttgtattgga
    10801 gctaatacgc cttctcttgt ttctccaact aataatcttc tatcaaaagt agctttgcaa
    10861 taatctggca ctactgatgc tcctggatat ggagaagatt ttatatctgt taaaactaat
    10921 attccatcac ctaaaactgg atgagttgga ggtactaatt gttgtatctt ttggattaca
    10981 ttagccattt tataaactgc atttacacct ttttctggat tagcagagtg agctggttta
    11041 ccaaaagttt ctactactat ttctcctcta cctctctgac caatctttaa gtttaactct
    11101 gaagactcac ctattacaac atagtcaggt tgaacagcct tacttatctc tctagccgaa
    11161 acaccttcaa atatttcttc atgaacaaca cctgctacat atagttctcc agcaaaatct
    11221 ctattagtgt ctttagcaaa atacgctaca gcagacatca ttgcagtata ttgacctttc
    11281 atgtcagaag ttcctctacc atatatcttt ccatcaacta tttttccttc aaaaggagga
    11341 acactccatt tacttgcatc tggaactgga actgtatcta tatgtccatc aaataatatt
    11401 ttcttacctg gcttgttacc ttttatacat cctactatat ttccatatct atctcttgac
    11461 caactatcaa aacctaattt ttcaaaagac tcttctattg cttttacaac accttcttca
    11521 tttccagaag aactaggatt ttgaattaat ttttggcata tttcagttac ttcttgtttt
    11581 ctttgttcgt ttaacatttc attatccccc tagatttttt ataatgtatg tattttatat
    11641 acttatatat tttctaaatt ttagaactta tatatcttct aaatatgtaa accaatatct
    11701 aataattgca ttttagtaat tccttatata cttggatatt ctccatccca aactatactt
    11761 ctatatttgt cagggtcagt atctccttct gtgcttatta aaagtattct tgaattttca
    11821 tctaatttta aatcttttct taaatctgct aaatcttctc tttccatagc tgctactaaa
    11881 agaccagttg taactgctcc agattctcca gaaattacct tttcatcacc atttaaagga
    11941 ttacctaatg ttctcatacc tttagcagaa acccagtctg gtgctgaaac aaatgctgct
    12001 gcatgatttt ttaatacttc aaatcctata gtatttggtt ctccacaagc aagccctgcc
    12061 attatagttg gcatatctcc tccaacaaat cttggttttc catctccagc ttctgctgat
    12121 ttatagtaac aatcagcttc atcagcctct actactactg ttattgggca ttcatcatat
    12181 attgaagcaa catacccttg aactgcacca gcaagtgaac caactcctgc ttgaacaaat
    12241 acgtgtgttg gtctatctac tccatattct tttaattgct ctatagcttc ttgagccata
    12301 gttccatatc cttgcattat ccatgctggt atttcttcat atccgtccca tgctgtatct
    12361 tgaaccatta ctccatggtc atcagcttct gcataatcat tagctaatct tactgcatca
    12421 tcataattta agtctgttat actagcatct gcaccttctc ctttaatttt atttaatcta
    12481 aattcagaag aaccttttgg cattaataca acagattttt gacctaattt attagctgtc
    12541 caagcaactc ctctaccgtg atttccatct gtagctgtaa agaaagttat ttctcctaat
    12601 ttttctttta tttctttcga agtcaatttg tcatacccta actcagatat atctgtatct
    12661 aatctttgag ctaagtatct acccatagaa taagaaccac ctaatacttt aaatgcattt
    12721 aatccaaatc tataagattc atcttttaca tatactcctg caacacctaa tttttttgct
    12781 aaaccttcta ggtttgctaa tggagtttct ttatattgag gaaagctttt gtgaaaattt
    12841 ctaaccttag ttatttcttc ttcatttaaa aatttgatac aattttcctt atcgcccttt
    12901 ggcaaattat ttactttcca tttaatttct tttaacatct aaaatacctc caaattattg
    12961 ttaaattttt atactttaat atataagcaa ttaacatgcc aatactaaat atatactaga
    13021 tatttttcca tgactcttta gtatagaaat tgcaatgctt ttattatttt ttcttttcta
    13081 tctttagttc atacaatgta tgtagaattt aataactaaa aaaccttttc tatcttctca
    13141 ctttttaatt ctcattttta aaatttaaaa ataaatctca aaacactttg tttctatatt
    13201 ttttaatata aaatttataa aatctaaatt aaaacataaa atagtgatgc aataatctat
    13261 tacatcacta tttgtatata ttcaataata taataaatct ttagtggtct ctaagaggtg
    13321 atttttcatc ttttttaaca tctaattctt cttttacgac attccatttt tcaagaccaa
    13381 acttagaaac cataactgcc tctgctgcac cttttccaag tctttttact gtgtccatac
    13441 ctgtctcatc ctcttgggca ccttttgctt ttccatcttt actccatatt gtaccaccta
    13501 tatagcatcc acctaaacct ccagacacta acatttcatg cattaggaag aaatttatta
    13561 taggaagttt tgtcaattct tggccaccat atctagttcc accagtagtt atagctgcac
    13621 caaccttatt ttgaagtttt cctggatgaa ctaaatacat tggacgaagt ctattaaata
    13681 ctgctgtaag ttgagcagtt atattcatat cataaactgg agaacctact ataattccat
    13741 ctgcctccaa tattttttgc tctaattctt ggatatcatc ttttataata cacatagttt
    13801 cttttcttat acatgcatca caatgtacac aaggacttat ttttttccct ctaactgacc
    13861 aatactcagt ttctatacca ggtatagttt ctgcttcttt caaagcttct aacactgcat
    13921 attctgttgc tcctttacgt ggacttccac aaatacctaa tatctttgcc ataataatat
    13981 ctcctcacca aataaatcta tttttattat ctatttacaa atacaataaa ttgttatttc
    14041 tgctttaatt tctactattt taattttatt ggtaatgaat gtatctctac atcagttgca
    14101 ttatatactg catttgcaac agctggcgca actggtacaa cggcacattc tccaatactc
    14161 ttaccaccaa aaggcccagc tttttcataa ccatctacaa atgcaacttg acattttggc
    14221 atatctacag ttctaaataa tttatatgtt ttaaaatcag tagctttagg tctaccttgt
    14281 tcatcaaatg ccaattcttc actcaatgcg tatcctagac ccatatgaat cccaccttct
    14341 aattgtcctt ctaaatttag aggatttata actctaccaa catcatgtac agctacaaaa
    14401 tcaactactt ttatatcctt agtttcttta tctactaaaa cttcagcaaa atgtacacca
    14461 taagaagtac gtccagctgg tgaagaataa tcttcaacaa cagttaactc tcttaatgat
    14521 ttactttgac tatatactgc gacatcagaa agtgatgctt ttacattttc atcaagttta
    14581 ctaattacac atccattgtc taggtataaa tcttcttttg atgtttcaag taatttttct
    14641 gcttcttcta atataagatt tttcaatttc tctgctgtct ttttagctcc tccaccttct
    14701 acaaataccc ctctacttgc atagtcacca aggttgaatg tacatgcatc cgtatcagtt
    14761 tcaaatgtat caactttatc aggtgttatt cctaaaactt cagctaccat cattgtttgc
    14821 atagttacaa caccattacc catatcatga gctcctgtta gtagtgtcaa cgtaccatct
    14881 tcatttaatt taagagtcaa tgtaacaaca tctctatgtg caccaaaaac tccattacca
    14941 tgtgcaccta ttgccatacc tatacctctt aaatattttc cttcttcttt tggaagtttt
    15001 ttcttttcat cccacttaaa catcttctta ccttgctcta tacaatccaa tactctagga
    15061 tttccaagag aaccgtggaa gattatatcg acatcatctg gctctactgc atttttattt
    15121 tgaaaatcga ctaaatccat acctatttcc tttgctattt tcgcaaattg agcttgttga
    15181 gccataaata tttgaggtga cccatagcct ctcatagctc ctgctattgg tgtatttgta
    15241 tatacaggca tcccagtaaa ctttatattt ggtattttat aaactttaaa tactttatga
    15301 ctaagtgctc ctataacatt taaagcactt gatgcatatg ctcctgtatt tgtatatatt
    15361 tttatgtctt gtgctattac ttcaccatct ttatttacac cagtttttaa ttttacaaaa
    15421 gacccatgac gagttcttgt tgatatcata gattcctttc ttgtaagaac cattttaact
    15481 ggtcttcttg tcatttttga taatgcggct gctactggtt caattgtaag ttctaatttt
    15541 ccaccaaatg agcctccaat agcaggtctt ataactctta ctttatttaa tgacatccca
    15601 aaaatttgag atagtattat tctaaatcca aatgtatttt ggcaaggtgt atgtacagtt
    15661 aatttatcat tataatcata gtgtgcaatt acactatgag tctctattgc actatgatgt
    15721 attgctggag tagagtattc atcttcaaat atataatcac attccttgaa gcctttttct
    15781 acatctcccc catttatgtt gttttgagaa actatatttg aatctccatg tatgcagata
    15841 gcatcttctt ttattgcatc ttgtatagta aatacagctg gcaattcttc atattcaact
    15901 tttataagtt ttacagcttt ctctgctatt tctggagtct ctgcagctac tgctgcaaca
    15961 cggtcaccaa caaatctaac tgtatctgaa aatataactt cattttcagg tattttgtgc
    16021 tcataaaatc ttaatgaacc attgtatttt ctttgtgatg tattgagata agtagctata
    16081 gctctaacac ctactacttt ctcagcttca cttgtatcta tactttttat ttttgcatgt
    16141 gctacagggc ttaaaagcat cttagcgtaa agtacatttt gtggtttcat atcagccacg
    16201 tatttcattt gaccagtaac ttttaaagct gcatctttta gagggtatga ttttcctatt
    16261 accttttcca taattatcta ccccctaatc tagctgcaga taattttaca gcttctataa
    16321 tctttacata tcctgtgcat ctacagagat tttctttaaa agcttctcta atttcatctt
    16381 cattaggaga tgcatttcta tttaaaagag cttgtgttgt tataatcata ccaggagtac
    16441 aatatccaca ttgtacagct ccgacttcta taaaagcttc ttgtattgga tgaagatttt
    16501 ctccattagc aaaactttct atagtgtata taactttacc ttcgcagttt tttgcaagaa
    16561 gtttacaaga tttcatgtca actccatcta tcaaaacttt acatgctcca caactgccac
    16621 tattacaacc ttgtttcgct ccagttagat ttaatttttc acgaagtaca tgcaaaagag
    16681 tttcattctt ttcaacttct atttcataca tgtaatcatt aatatttaat tttatttttt
    16741 ccattttctc ctcctacaga taaactaatg ttgtaataga atattattac aatcttattt
    16801 taatattagt ataagctatt ccacttatgt ccttccattc tattagttac attagctctc
    16861 ttacataatt cttctgctgc atgttgtgca ttaagcaaca cttgttcttc actttcaatt
    16921 gtagttactt ttccattttc aagtattaca tttccatcaa ctaaaacact ctctacattg
    16981 tgcatactag ctgagtatac caaagttgat acaggattgt gaagaggtat tgattttgga
    17041 gaaagcattg agttaaatat tactaggtca gcttttttcc caatctccaa agaacctatt
    17101 tcatcttcca ttctaagagt tcttgctccc tcaattgtag ccatttcaag aactttctct
    17161 gcagaaattg ccagagggtc acaattattt actttgtgtt gtaaagctgt taatttcata
    17221 agctctacca tatcttgtgc gttattactt gctgcaccgt caagacctaa actcacattt
    17281 ataccttttt ttaacatttc tggcacaggt gcaacaccag aagcaagata catgttactt
    17341 gctacattat gagaaacttt tatgtcatac tttttaagca tttccatgtc ttcatcagtt
    17401 atacatacac aatgtactgc tacaacttct ggacctacta taccccattt ttcaagtaat
    17461 tttatatcta tttctccatg tatatccttt gttgctgttc ttgcaaattc tgtttcagat
    17521 atatgaactg taaaatatga atcatattct ttaactatat tccaaagcat cttacccatt
    17581 tcttcagata tagcccacat tgaagaagga gctacgcaga tttttattct tccattttct
    17641 gaattatgat atttttcgaa tacccttctc aaatcttttt caaccgtttc cacatcttct
    17701 ataagctctt tgtgaatacc taaatctata caacctctac ctacaacccc tcttatacct
    17761 aaatctttat atgcctttac tattccatct gtaagtcctg gtctattgtg agtatgcata
    17821 taatcaacca ttgtagttat accacttttt aaaccttcca tacatccaag cattgcagca
    17881 tcataagtat cttgttctgt taagaatttt gctgatggaa acatcattgt atttaaccac
    17941 tcatgaagtg ccatatcatc acctaaacct tttaataaca cttggaacag gtgattatga
    18001 gtatttataa agccagggaa tattactttt ccattagcat ctataacttt tttagcatca
    18061 agatacttag gctcaatatc aactgtattt cctatgtcca agattctgtc accttgaata
    18121 gctattgctc catcaaatat aacatctctg tctttattaa ctgtaacaat tattgcattt
    18181 tttattagta tatcaatcat cttaaatccc cctttgaata ctttcattac aacattttac
    18241 catatggttt tctcatatta tatctaatac atttatccaa tattttcctc aagatttgaa
    18301 gtaacgtttt cagcactttt acgtgacttt aatttaaata atgtttgaag taatatagct
    18361 attattgctc cagaaactat acttgaccct ccaaggacag ttccaattgt ttctgggaat
    18421 tggtctaaaa cttgtggagc agttgtaaga cccatacttg ttgctataga tacacctgct
    18481 ataagattgt tttcttcagt gaatccatcc atactcataa tagagaaacc agacattgct
    18541 attgatgaga aaactactaa agttgctcca ccaacaacac aagatggtat agttgtcatt
    18601 attgcaccca atgctggaga tatccctgct agtaataata caagtgcccc tagtgctatt
    18661 acaaatctac ttgtaacttt gttcatagat acaattactg tattttggct aaatactcca
    18721 gttggaagag ctgcaaatag tgatgaaatt atagaagtta tagaatttcc taatacaact
    18781 gatgctaatt cttcgtcagt tacctctcta tccattgctc ctactgtagc tattgtacag
    18841 gcacccatca tatcagctat acctattatg taaataatag taaacattac tattaactct
    18901 ggtctaaatt ctaatccaaa agctgctggt gttggtaaag atactatagc tgctccttga
    18961 acggctgaga aatcaaccaa tcctaaaact aaagatatag cataacctac taatattcct
    19021 attaatattg aagaattttt tactaaacct ttaccgtatt tatttatcat tattattaca
    19081 aaagcaacta taactcctac tgtgaagttt attgggcttc caaatgtttg tgtgccttct
    19141 cctccagcta aattctttat agctgttgcg aatagtccaa gtcccataca tgctattata
    19201 gttccagaaa ctattggagt gaatatatgt ctaattttct taactccaaa tcctacaaag
    19261 aatacgatta ccccagcagc aatctgtgct ccaaatagag ctgctaaacc atctttagct
    19321 gctactgaaa gacaagctcc taaaaataca taactcattc ccatcattac aggtaatctt
    19381 gaacctaact taaatccttt aaataaaggt attggatata actgtaaaaa cgttgctaaa
    19441 cctgctgcta acatagaacc ctgaatcaac attgttgctt gcccctggtt aagtcctacc
    19501 acgtttgcta ttaatatcgc aggtaccata tttcctacta tcattgctaa cagatgttgt
    19561 aaggccaaag gtattgattc ctttagtggt ggtatcccat cgacatcata ttttgaagta
    19621 ttacgtgttt tcataaaata aatcctccca tacataaata tcttgactaa atatttttat
    19681 aattattttc tataaatatt tttatgacca tgaatttatt gctcaaccga taaacacagc
    19741 taaactgagc tatttttata aaaaattaac aaatttctaa atattgcctc cccgattttc
    19801 aacttaaatg aacattaatt gcctggtata tatttatttg ttaaattttt tattaaaatg
    19861 aatagctatc aaaaataatc taacaatctc atactaaata atttaaacaa ttcttttaaa
    19921 ttattatatc ttaattagtc tcttccataa cagctctttc tttagcctca tcctctagac
    19981 ttttatttgg tattattaga tttaaaacga atgcaaccaa tgttgcaatt actattggag
    20041 attctccaaa tactaacttt acaaatgaag gaaatgcctc ttgggctgct gggactgatg
    20101 ttattcccat gcctaaggca attgccaaac caacaatggt aatatttctt gatgacaact
    20161 cttctgtaat aattaacttt atacctgtca ttgttatcat actaaatata gatattgtag
    20221 ctcctcctaa aacagaatat ggtatagtag tcattaatgc accaaattta ggcacaaacc
    20281 ccgacaataa cataaatata ccagctattc caagcacaaa tctacttatt actttgttca
    20341 tagctaccat ccccacgttt tggctatatg atgatggtaa acctccaaac aaagctccta
    20401 tcatagtaca aattccactt cctattactc cacttgataa ttccttatct gtgatattcc
    20461 tatccatgcc tcccattgta gtagctgata agtctccaat tgcttgaaca gcattgatta
    20521 tacttacaat tactactgag attattacag gcatatcaaa ttccatccca aattcaagcg
    20581 gtttaggtat cgcaaaccaa gttgcctctc taatagggtc aaaatttatt atacccagaa
    20641 ctaaagatac acaatatcca actataattc ccaaaaatat tgctgccaat tttgcatatc
    20701 cttttgcaaa ttgactaaga cctataacta ccactaatgt tatagccgct actgcccagt
    20761 tttgcataga ggcataagta ggactattta caccacctgc catataattt attgctatag
    20821 gatacaaaga taatccaatt gtaaaaacca ctgttcctgc aactatattc ggaaaaaatt
    20881 ttcttatctt ttttatgaat atccctatta atactgtagc cactccccct attaattgtg
    20941 cacccaaaat gcttgctaat ccataatttc ctccgactgc tacaagtgtt ggtacataag
    21001 taaaaccaac tccaaaaatt ataggcaatc ttgaaccaat atttcctatt ggatatagtt
    21061 gtataagtgt tgatatacca gctattatta atgcatattg cactaaaagt gttatttgtg
    21121 tagggctaac acccacagct cctcctacga ctataggtac tgtaacagtt ccaactatca
    21181 ttgctagtat atgttgcatt gatagtggca aacactttaa aattggtggc cttccatcaa
    21241 tatcaaacat agaaacattc ttttgatatt tactcaaggt tatcactccc aactattttt
    21301 aatgacagtt attattatct cacacaacat cctttctttt tttattttgt catctcaact
    21361 taatatgaag caattataat gccaattttt taacataatt gttttacata tataattttt
    21421 atta
//
LOCUS       DAELNY010000051        21417 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.051, whole genome shotgun sequence.
ACCESSION   DAELNY010000051 DAELNY010000000
VERSION     DAELNY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.051"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(464..2068)
                     /locus_tag="KPE55_001643"
     CDS             complement(464..2068)
                     /locus_tag="KPE55_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0492732.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2454..3380
                     /locus_tag="KPE55_001644"
     CDS             2454..3380
                     /locus_tag="KPE55_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0492733.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3500..4498)
                     /locus_tag="KPE55_001645"
     CDS             complement(3500..4498)
                     /locus_tag="KPE55_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG0492734.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4602..5039
                     /locus_tag="KPE55_001646"
     CDS             4602..5039
                     /locus_tag="KPE55_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0492735.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5125..5712
                     /locus_tag="KPE55_001647"
     CDS             5125..5712
                     /locus_tag="KPE55_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0492736.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6012..6422)
                     /locus_tag="KPE55_001648"
                     /pseudo
     CDS             complement(6012..6422)
                     /locus_tag="KPE55_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(6540..7658)
                     /locus_tag="KPE55_001649"
     CDS             complement(6540..7658)
                     /locus_tag="KPE55_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0492737.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKVRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTNVDIEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            complement(7660..8067)
                     /gene="tnpA"
                     /locus_tag="KPE55_001650"
     CDS             complement(7660..8067)
                     /gene="tnpA"
                     /locus_tag="KPE55_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0492738.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(8636..8845)
                     /locus_tag="KPE55_001651"
                     /pseudo
     CDS             complement(8636..8845)
                     /locus_tag="KPE55_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(8947..9495)
                     /locus_tag="KPE55_001652"
                     /pseudo
     CDS             complement(8947..9495)
                     /locus_tag="KPE55_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9854..11764)
                     /gene="phnW"
                     /locus_tag="KPE55_001653"
     CDS             complement(9854..11764)
                     /gene="phnW"
                     /locus_tag="KPE55_001653"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG0492739.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(12043..12828)
                     /locus_tag="KPE55_001654"
     CDS             complement(12043..12828)
                     /locus_tag="KPE55_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0492740.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(13045..13275)
                     /gene="dltC"
                     /locus_tag="KPE55_001655"
     CDS             complement(13045..13275)
                     /gene="dltC"
                     /locus_tag="KPE55_001655"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG0492741.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(13294..14442)
                     /gene="dltB"
                     /locus_tag="KPE55_001656"
     CDS             complement(13294..14442)
                     /gene="dltB"
                     /locus_tag="KPE55_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG0492742.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWCYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(14442..15956)
                     /locus_tag="KPE55_001657"
     CDS             complement(14442..15956)
                     /locus_tag="KPE55_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG0492743.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYRELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(16077..17267)
                     /gene="dltD"
                     /locus_tag="KPE55_001658"
     CDS             complement(16077..17267)
                     /gene="dltD"
                     /locus_tag="KPE55_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG0492744.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(17555..18574)
                     /locus_tag="KPE55_001659"
     CDS             complement(17555..18574)
                     /locus_tag="KPE55_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0492745.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(18750..19289)
                     /locus_tag="KPE55_001660"
     CDS             complement(18750..19289)
                     /locus_tag="KPE55_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG0492746.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(19390..20070)
                     /locus_tag="KPE55_001661"
     CDS             complement(19390..20070)
                     /locus_tag="KPE55_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG0492747.1"
                     /translation="MQNVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(20099..21388)
                     /locus_tag="KPE55_001662"
     CDS             complement(20099..21388)
                     /locus_tag="KPE55_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0492748.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         7009 a         3676 c         2271 g         8461 t
ORIGIN      
        1 ttttttaaat tagaattatt ttaatgaatg attcaagtaa taggtatgaa attgaatctt
       61 ttagaactgc ttacaaaaaa tactcaaaga aaaatagtaa ttttctcaga ttcacaatat
      121 attttatatg aaatgttata aggtaatcta tttaataaat ttaaatttgc tattatagat
      181 ttagttccaa agtcttactg caaagtaata tacatagttt tttaataaac taataagtgc
      241 aattaataac tacttaattg tttaatatag tacatagata actttaataa atctacagtt
      301 atttaaaaag gtagaatcaa taaaattata cttataaatc ttcaatacaa taattaaaat
      361 ttaataaaaa tataattgtt gatttaactg taatcttatt taattctacc taacttatta
      421 tataaaacta ttttttatag attataaaat atctaacttt catctacttc aaagaataca
      481 atacttctcc ttctcttata gtctgcaata ctttaatatc ttttattttc atagggtcga
      541 ttgtaagtgg gttttcatct aatattatca aattagctaa tttcccttct ttaattgaac
      601 cttttttatc ttcttcaaaa tattgatatg ctgcatttat agtaacaccc tttaaagcat
      661 ctaatgggct tatcttttca ttttctccta tttgtatacc tttttttgta attctattga
      721 cagcacacca tactgtttcc agcatattag gtgctattac tggtgtatcc tgatgaagag
      781 tgtaaattaa tcccttttct atagttgttt taagtgggct aatcttaaac gctctatctt
      841 ctccaaggtt tttgatatga atatcacccc aataatatgt gtgagctaca aagtatgaag
      901 gtatcatatt tattactttc atatcatcaa tctggtcttc tcttactgtt tgagcatgta
      961 tcataactgg tcttatatga ttttcactac tttgctcttt taagtttaaa actttctcaa
     1021 acgcatctat caactggtct gctgctgcat ctccattaca atgagttaag agttgcatct
     1081 tttcttttag tgaaatatct ataaactttt ctacttcttc atctttgtat ataggatatc
     1141 cacaataacc atcatctgaa ttttcatatg gtctactcaa ccatgctgtc ttaccttgtg
     1201 gagaaccatc taagaaaatc ttataacctc ctattttaaa tctatttata tattttttta
     1261 taaactccct attattatct gctacagact tacttttttt caaatttaca tatcctacaa
     1321 catccatttt taatttattt tgatttgcca ttgcttttaa aatgttaaat tgattttctt
     1381 ccattaaacc ttcttgagct gttgtaatac cataacttag atatatgttc tgtgcttttt
     1441 caattgaatt aaaaatctca tttgatgatg gttgttttat tttagatgct acattaaaaa
     1501 atgcattttc ttctaaatat ccatttggtt cttcactgcc ttctactcta cctatatgtc
     1561 caccttctgg gtcacgtgtt tcattggtaa caccaagctg ttttaacccc aatgtatttg
     1621 caactcccat atgaccagaa gcatgtgaaa ttagtattgg attttctgaa gatgcactat
     1681 cgagtacact cttatctgga tgcttatttt cttctaaaaa attattatca tatgaaaatc
     1741 caattatcca gtctcctttt tttatattat tcgactcttt gaaatcctgt atttttttta
     1801 ctatgtcttt aaaactggtt gcatcttcta aaggaactag ctttagagta gtagcaaaag
     1861 ctatcaagtg actatgtgaa tctataaaag atggcatcaa tgtcttacct tgtaaatcta
     1921 taatttctgt atctttattt ttcaaagcaa atacttcttc ttttgttcct atctttttta
     1981 ttattttgtc ttttataaga atagcatctc cacatattga atcttccatt gtgatgatat
     2041 ttccattgta ataaattttt tctttcataa aattctcctt acgttgtcta ctttaataaa
     2101 tttttttcat tattttctgc ttaattatat tatcttcttt ataaactttt tctttagagt
     2161 ttaatcatat aaaagtttta tttttaatta ttgtttgcca agaagtaaat actattcatt
     2221 gtaaataaga caaattaata ggctttatac taattgaaaa aatattataa gttgttaaat
     2281 ttaaatatct aaactcaatt atatctgtac aattgtaatt ttttaaatca tgatgtatta
     2341 ttttaaatat acagttacat tattattcac aatataagta tcttagttct caaataacat
     2401 ctcatgtaat ctattcaatg caaaaaattt aaatattttt taggaggttt tttatgagca
     2461 aggaaaataa aaatgtaaga attgctgttg tccaggcgtc accagtcata atggatttag
     2521 aaaaaactgt tgaaaaagca ttaggattaa ttaaagaagc tggtagaaaa ggggcaaata
     2581 tcgtagtttt ccctgaagca ttcatacctg catatccaag aggtctttca tttggatttg
     2641 ttgttggaag tagaacaatg gaaggtagag aagattggaa aagatactat gataattctg
     2701 taccagttcc tagtgctaca acagatttat taggaaaagc agcacaagaa gcaggagtat
     2761 atctatcaat gggtataaca gaaagagatg gtaatgatat aaattgtacc ctttattgca
     2821 caaacttatt tttctctcca gaaggtaagc taattggtaa acacagaaaa cttaaaccta
     2881 caggaacaga aagatgtatt tggggagaag gagatggaag tactcttaca gtagttgata
     2941 ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa ttatatgcca ttagctagaa
     3001 cagctcttta tgctaagggt gtaaaacttt acatagcccc tactgcagat tcaagagaag
     3061 agtggcaagc aactatgaag catattgctt tagaaggaag atgttttgta attggatgca
     3121 atcaatatgt tgaaaaaaat atgtatccaa ctgaccttaa ctactataaa gagctagatg
     3181 cagaaccaga aattatgtgt ccaggaggaa gttgtatagt tgaccctttt ggaaaatatg
     3241 tagcaggtcc tattttcaat aaagaagaaa tgctaatagc tgatttagac ctagaaaaaa
     3301 tagtacttag tagactagat tttgattcag aaggtcacta ttcaagacca gatgtatttg
     3361 aattaattgt tcatgaataa tctttttaca caaacatcta cataaatctt tttacataaa
     3421 aaaatgagat actcataagt aaatttcaag tatctcattt ttgcttaaaa cctataatcc
     3481 taattataaa tttattcacc tatatttttt ctaaatcaac tactgttctt ccaacatgtt
     3541 ttcctgccat tattgaatct atactatcat ttaactcttc caatgatact acatttacca
     3601 tatcatctaa atcatctaat ttccattcat ttgataataa atcccatatt tttaatctta
     3661 agttcattgg gcaatccaca gaatctatac catataaagt aattcccttt aagataaatg
     3721 gatatactga acttgtaaaa tcaactcctc ctgcattacc acaagttgtt actgctccac
     3781 aataattagt tgtctttatt gctgttgcta gagtatttcc tcctactgta tctattactc
     3841 cagcccatct tggtctaagc agaggtcttc ctgagttatc atccacttct gacctatgta
     3901 taacatcttt aacacctaag tttagtaata cttctttttg ttcttctaat tttcctgttg
     3961 aaactacaac tgaataacct aatttaatca atattttagt tgcaattaca gcaactcctc
     4021 cagtaccacc agtgactaat acatctccca tattcttgtc tacagaatct ataaatttat
     4081 acacagataa agctgctgta aaccctgctg taccataatc cattgcttct tttagtgaaa
     4141 gatttttagg aagtttaact acccaactag aaggaactct tatgtattct gaaaaaccac
     4201 cagaagtatt cattcccaag tcataacctg ttactaaaac ttcttctcca attttaaaat
     4261 catcacaact actagattct actataccag atgcatcaat tcctggtgta tgaggatact
     4321 ttctcgtaac acccttatta ccatttgcag aaagtgcatc cttgtaattt aaagaagaat
     4381 attttacttt tattaaaact tcgccttctg gcaatgtatc cacttctctt tcaactatat
     4441 ttcttctaaa atttccttct tcttctgtaa tatatagtga tttaaatttt ttattcataa
     4501 atttcctccc aatacattta gttttatgtt aaactaatta tagtttataa atagttttat
     4561 gtcaaactaa tttaaataga ttaaagattg gagaataatt tatggaagat aaatatattg
     4621 tacatttcat aagtaaaaca aaggctagta tgattaaatt cattgaaaat aaactcagta
     4681 aaaatggttt aggtgagtta atacctactc atggaaatat tttaactgca ctttatgaaa
     4741 ataatggaat acttacaatg aaagaaattg cagctaaaat aggaaaagat aaatccactg
     4801 ttacagtgtt ggttaataaa cttataaacc taggatattt agaaagacaa aaatgtacaa
     4861 atgacaaacg tatcacgtat ataaagctta caaaaaaggc actgcttata gaagatactt
     4921 ttaatagcat ctcaacacaa gttaaagaga cggcatacca taatattaca gaaaatgaaa
     4981 aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa ttttaaaaat gctgactaaa
     5041 ttgagcataa ttatatctcc tattggaata ataaataaaa agaatcaaat attgtaaaaa
     5101 tttattgtga tagagaggta gaatatgaat ttaaaagata gtaaaactaa agaaaattta
     5161 ttaagagctt ttgcaggtga gagtcaagct agaaatagat acaatatttc tgcatctgta
     5221 gcaaaaaaag aaggattata tataatagag tctttattta attacactgc tgaccaagaa
     5281 aaatcacatg caaaacaatt cttttctaaa ttaaaagaat ttgctggtga aaatataagt
     5341 atatcaggag cataccctgt agatttatat gacaagacag cacaacacct taaagctgca
     5401 caacataatg agcttcaaga gtgggatgat gtatataaaa actttggaaa aacagcacgt
     5461 gaagaaggat ttgaatctat agctaagtta tttgaaaata tagctgaaat agaaaaagtt
     5521 catggtgata gatttggtag atatgctact gatttagaag aaggtacttt attcagaaga
     5581 aatgaagata tacaatggat atgtacacat tgtggccata tacatatagg taaagaagct
     5641 cctaaagctt gtcctgtatg tcttcatcca caaggatatt tcttagattt taaaaactca
     5701 ttatttgaat aaactaaggt aatcattgta tttaaacaat ttactatatt tatatcaagt
     5761 atttttataa atactgtatt atcttaataa caataaaatg aaccattgat attactataa
     5821 gtagaaaatg tagtttaaat acaatttata gtagttcatc atggttcatt ttattgttta
     5881 ctaatcccta taacttactt aaatctatct ttaactttta catactcaca taaatcctca
     5941 tctctaaact aaatatttaa ataaagtgta ttttaagtag attattataa tacattatat
     6001 aagataatac actatgataa ctttatactt atatcattta tttcttttgt caagtcaacc
     6061 tcaataatct taactacatc tttttcaaac tttacttttt tataatccag acatcctact
     6121 acgaatggaa ctccaaactt tctaaacaaa taaatctttc cactttcatt gtatattgat
     6181 gtaaaatttt cttctattat attcactatc aattttaact cttcaaaagt tattatagat
     6241 ttaactaaaa acttctctat tatgctcaat ataagcagtt cttttttacc tatgctttgt
     6301 tcttttttat ataaattttg aaatatttct ttggtataat tacatataac ttctttactg
     6361 agaataaaat caatgtcaaa ttctttatta atatcctttt tcataaacat atttgcaata
     6421 tcctgaacct cccatgacta aagtcacagg gttcctgctt catagaattt tgcatactaa
     6481 aatatgtctt actaattctc cacaggctat ccccgtagtt cctacggttc ttacataagt
     6541 tatttatttt gaattgttat tagtcttaat ccttctttta atatatttat acttgcattt
     6601 atatctctat cgtgagtttc attacaacta ggacaaatcc attctcttat atttaagtcc
     6661 ttcatttcct cgtttttata cccacattta ttgcatattt gtgaacttgc aaagaattta
     6721 cctaccttta caatttgtct accatgccaa ttagccttgt attttaattg acgaacaaat
     6781 tctgaccatg acacatcaga aattaagcga gatagtttac gatttctaat catattcttt
     6841 acttgtaaat cttctatgca aataatatca ttctctttta ttaatttagt agatagtttt
     6901 tgtagaaaat catttctttg attagctata tgttcttgaa gtcttgcaac ttttaatctt
     6961 gctttatttc tatttaaact accgattgtc tttctcgata attctctttg taatttagca
     7021 agtttgttta atgacttctt aagatactta ggattttcta taaagtctcc acaacttgag
     7081 atacaaaatt ctttaattcc taaatctaaa cctatctgat tattagtatt ttcaaatact
     7141 tcaatatcta cattagtaca gcataatgat acataatatc taccacttgg ttctttagat
     7201 atagtagcat taagtattct accttgaggt acttgtttat cccttacctt aaccatacca
     7261 agtttaggta gttttatata ttgaccacaa tacataatgt ttccatttgt aaagttagtt
     7321 ttatatgaaa atctattaat tttctttgat ttaaatttag gaaatcctgc tttttcttta
     7381 aagaattttt tatatgcatt atctaaatct tttaaggcgt tttgtagtga aaatttgtca
     7441 ggttccttaa gccattttaa ttcttttttt aaattggtta aatcagaact acactgttta
     7501 taagtaaaag tttctttatt gtttttataa acttctattc ttttagcaag atacttatta
     7561 tatacaaatc tacaacatcc aaaagttttg ttaattaact cttgttgttt tttatttgga
     7621 tacattctaa acttataagc tttttccact actgccacct cactttactt gttttttctg
     7681 attattaata tattttctta tctgttcttc tgtattttct gatacagtag caacgaaata
     7741 actaggattc caaagatgtc ctccccataa tttctttttc aactgttctc catgtttttt
     7801 cattaacagc cttgctgata caccttttaa tgcttttatt atatctggta tatagtgttg
     7861 tggtgaacaa tttattagta aatgtatgtg atctttatca ctattacatt ctaatatttg
     7921 aaatttatta tcttcagcta ttttgtttaa tatattaatt aaatcttctt caatttcatt
     7981 tgttaatact ggatgtctat attttacaca ccatactata tggtattgta ctaaatacac
     8041 ataccctcta ccatgagtca cttccatttt tcatcaccca acataatatt aacatagtaa
     8101 ttacttaaaa caagtattct taaggttttt atatttattt ttaaataaca tacgtaagaa
     8161 acgcaaatct taacttttat catttttgag gttgtcgttc acataagttc gctactacct
     8221 atgcagttct cttatgaact tcttacactt tcatgcaagc acagactata tcttatcctt
     8281 cggcattatc cgttaaggtc tacccacttc cactaccgat agcttgtagt gtacttccct
     8341 caagaggaat agtcgttgaa gtttttccta ttcggagctt acctgctgat tgcccatttt
     8401 aaagtactta ggatttaacc ttataccatc taaccaattt tttctacttt cgtaactttc
     8461 acacttaggt ttatttcatc cttatgtttt agtttggtta gctttagggg tttccagcaa
     8521 ttcaagtagt attggatagg ttctatccta tctctacata taagtttccc tatatgctga
     8581 ctattttagc tactaactca tgactaaagt cacaagtgtg catagctatt ttttaatcaa
     8641 gagaagtctc ttctttcata gacaatatta actctattct ttctagtatt ttatctcttg
     8701 gaaagaaagt ctcttgacct gtaaaagctg atttttttat aaaccattcc tctggtatta
     8761 tgttttttct tttccatcta tataattgtc cataagatat attagttttg ctcaataaat
     8821 ctttcttaga tattaattct tccatagctc ttctagccca cctttatact gttttttaca
     8881 gtagaattat ctgaaactga taaattatca ctgtattcaa tatttccaac atcataatta
     8941 tctactagag atatgctttt tttctagcat acgaaatttt ttgcaataat ctccatgccc
     9001 cttttttata tttatactac ttgctccaac ttcattaaaa ttagaagttc catttaaact
     9061 aatattaact tcttcacaat tcaaaaatcc attacactcc aagattccac ttaaattgat
     9121 attttcacaa tctatattct catttgcttt taaatatcct tctccatgaa taatatccga
     9181 ttttatactt ccttctacat taataattcc actagtatca atttcaacag aattaatatt
     9241 tcctgtacat ctaagaagtc ccgatatctc aacttttgat gccagtatgt ttccttctac
     9301 tttacttaaa ccagatgtaa aaaattcatt acattctgtt gtcccaaaaa cttttgctga
     9361 accagaagaa tcaaatttac tacattttac acttccttct attttgcctg ttcctgatat
     9421 attaacatta tcaaaaattc cacctttaca ttttccaaca ccatcaaatc ttagtgaact
     9481 taattcttca ttcataatga tatcctttct ttaccgtatt ttatctttaa acctaatata
     9541 acaaaacatt gttacgttgt caagtatgat aataatactt aataaagcaa ctatcattta
     9601 ttttctataa ataggcataa aaataatagg gaaatgcttt atttaaacta taaatctaat
     9661 ttcttagatt gtggtttaaa taaaatatat ccctattact caatgtttat atattcattg
     9721 tataaacttc taaaatataa gtctttaaat tctactgtca atacttttta tacactgttt
     9781 gtgctatcat ctcacacacc taaaggagta ggctttgcgc actattctgt aacctacaag
     9841 acactatctt cttctatcta ttgataaatt tttctattac atcagctagt ctctccatat
     9901 cagttggata gacttcacct atgctaccta ttctaaatgt atctatatca gtcaatttac
     9961 ctggatagat aacaaatcca ttatctttta aataagtata aaattccata aattcaaact
    10021 ttgcattttt aggatataag aaagttgtta ttacaggtga ttgagcatta tcattcacca
    10081 aagtatcaaa gcctaaagat tttaatcttg aagctattgt aaactgattt tctttatatc
    10141 tagcatatct tttttcaaca gaaccttctt cttccaattc taataaagct tgataaaaag
    10201 ctcttacaac atgtgtaggt gaagtaaatc tccatttacc attatttttc tccatagtct
    10261 cccattgcgc atacacatca agagataatg atttagctat acctttacat ttagataatt
    10321 tctctttatt agctattata aaaccaaatc caggtactcc ttgtatacat ttgttagaac
    10381 tactaactaa aaaatctata ttaaaatctt caacatcaat ctcaattccc ccaaaactac
    10441 tcatagcatc aactatataa attttgttat attcttttgc aagtttccct acctcttgta
    10501 taggatttaa tctacctgta gtagtttcac agtgaaccat tgatatatgt gttatatcct
    10561 tgttttcttt taataaattt tctactacat ttaaatccac tgcttctata tctttaaatg
    10621 tgcagtcaac aaattgtatg ttaagataat tacatatatc tttcattctc tttccatatg
    10681 ctccatttgc aattacaaga agtttaccat cttttgaaat agtacttcct attatagctt
    10741 ctactgaaaa agtcccactt ccttgcatta atacacttgt gtatttatct gtattttggg
    10801 ttgctagaga tactaatctt cttctaacat cttgtactag gttattatat tcaacatccc
    10861 aagtacacca atcttttaac atagatgctc ttactgattt agtagtagat agtggtcctg
    10921 gtgtaagtaa tatgtaatca ttttctggta caaaatctga tttaatagtt tcattctcta
    10981 tttttattag tatatcttct aactcagcca tagtttctat tacaaaatgt gcacctgcct
    11041 ctttaaattt ctttgaaact atactcatct tagcttttaa ttcttcctta tccatgttct
    11101 ctacttcttc ttgtgtaagc cctaactcac ttgaaccttt tattacagca acactccaca
    11161 ttcctgcatt tacgccttct tttacatcac ttattgtatc tcctactttt accatagaac
    11221 tcataggaga cacccctaaa gcttcagcat ttttatagca catccaagga tacggtcttc
    11281 cttgagatac ttctgatggt gttacaagaa aatcgggtga gtatcccttc tttgctgcat
    11341 ttggttcaac tatgttcatc atctctcttg tataacccgt tgtagaacct atttttaatc
    11401 cattttttct caacttttca atagtttcaa caacatgagg aattggagtc gtatactctt
    11461 ctaatgtctc aaataacatt ggctcaaatt cagcataaag ttcatttaca tcatcttcag
    11521 ttggcacttt accaaactta tctgaccaaa tattctttat tctatccatt tcacacattt
    11581 ctcttatatg gtctattttt aattttccca taggttttct tgcttcttcc attgttacat
    11641 ctatacctct tcttttaaat atctctataa atacattaag aggtgcaaaa catccataat
    11701 ctactgttgt tccagcccaa tcgaatacta ctgcttttat tttttctcca tatatctttt
    11761 tcatatttat agcctccata gattcttttt agaatttttg tgtttttaat tgtttttact
    11821 tttaaagtat aactttcata tgttaattat acatcatatt taggttaaat atatattaaa
    11881 tttctgtttt tagttgtatt tagttgtttt tattttgtat catattccca taaaatccta
    11941 agttacaatc taaaatttac tttctaatct taatctatga actgactata ttttatgaat
    12001 ttagttgtat tgagcatagt tgtattatat aaaactaagg gtttattttg cttctatcca
    12061 tgttatattt atctcattta acttatcctt ccattcattc ggaggttcta catcacaaat
    12121 aacaaaatct aattcctcta aatctgctat cttatacata ctagcaattc ctattttaga
    12181 actatcacat aatacaaaac tttcctttga attttttata tattcttttg acaacaatgc
    12241 cttattttgg tctatactac taattccaaa actatcaagt ataccttcac tagatataaa
    12301 agctttatct acgtataagt ttttcatagt atctattgac attggacatg ctgtcctttg
    12361 gtgttttaaa tttacttttc caccaataaa tataacttct ccatcaaaca aaccttgatt
    12421 ctcataagaa atcaattttg atagaactgg ataagagttt gtaataattt ttaaattgtt
    12481 tacagatatt atataatcta caatctgcaa attggtagta ccttcatcta tgataattga
    12541 ctcattgtca tttacaaact tagctgcata tttagcaata ctttgctttt tatctaggtt
    12601 agtattgttt ctctttatat gttctggttc aatcactcca taatttattt ttatagcacc
    12661 accatatacc ttttttaatt tattttcatt ttctaattct tccaaatatc ttcttattgt
    12721 ttctgatgat acttctaatc tttttactaa ttctttagtg tttaccttcc cttttgaatc
    12781 taataattta agtatttctt tttttctatc tatcgaattt aaagacattc attttcctcc
    12841 taaatataat atatttttct cctataatat tacaatgata cctaagattt atgaaatcat
    12901 atttatgtat taaatttttt acttaattaa agttattttc aaaagacttt gtattatcat
    12961 taaaaaagtc catcaaatat tagtatcaga tggactttgt aaaattttat tttataaatt
    13021 aatatttaaa gtctaactac taatttattt tcttgtctcc aagaatttta ctaaattatt
    13081 tacagtagac atatcttttc tctctaaatc agttggttga agctctatcc ctaatctatt
    13141 ttcaatttca agtaaaactt caattatagc taatgagtct aaaagttccg tctcaaataa
    13201 attaaggtct aaatcttctc ttatctcatc agtccctaat acatcttcaa atatttctat
    13261 aactgtttct tgcataaatt ttcctcctta aaattaagct aaataacctg agaatatcag
    13321 caatccaaaa cacacaatat ggaatgtaat gaaaatctgc actctctcga accatttatc
    13381 ttttttatgt tttttataaa atttagattt tcgctgatat atatcagtta acacaagtgc
    13441 caatccttgg tatacaccat aagcaacata acaccaagtt agaccatgcc aaaatcccat
    13501 tgttatcatg gttatcatct gagccacatg agcagcagta gtccttttct taaatctttt
    13561 ctttctcatt gatgacataa caaatctaga aaaaatataa tctccaaacc atcttgaaag
    13621 acttatatgc catcttgtcc agaactcttt catatctttg ctgataaatg gcttgtcaaa
    13681 gtttataggt acttgtattc caaatatgta ccctgtacct atagcaaata aactatatcc
    13741 cgcaaaatca aagaacaggt ataaactata tgcgtacata taacttaaaa tatttataaa
    13801 actcatatct ttaggtattc ttgataccca atatgtattt atcaaaaatg ctataacaaa
    13861 cttataacct actcccatga caatattttt tattcctggc agtaagtatt cttctatata
    13921 ctcttttcta gagatttgtt tttccaagtc ctgttcaaat cttcttgacc tatcaatagg
    13981 tccagaactt aaagttggaa aaaataacat aaaataaagc atctttgaaa ttttaacttc
    14041 ttttatagct ccatcataaa tttcaataac catttgtata gttctgaaat ttaagtatga
    14101 tattccaata aatccaatta ttccaaaaga agtaactgga gatattttat ttattattat
    14161 tgggagcatt gatgcaaaca agaatcccca atatatgtat ttatttttag tttttcttct
    14221 tacatattca tatcctttaa ctattattac ttcaagaata ataaatatta ccaagtactt
    14281 tagttggaca tcaattccta ctattaagaa tatcataaac aaacttgcca acataccata
    14341 atactttata ttcttgccca ttagacctaa aattactgct ggtattgatg ttaataataa
    14401 tatataaaga taaaaataat ctccatattg tgaaaaaatc attaaatttc ctccataagt
    14461 ttctttctgt ctattttacc attgatattt gttggaaact cagagattat tgaaatattt
    14521 cttggaatca tatatgaagg tatatacttt ccaagttcct tttttattat catcccattt
    14581 ttaatattac ttaaatcatt cttttcattt aactcaacaa taccttttaa gtaagcaatt
    14641 ttttcgtcct tatatactgg taaaactact gcatttttta cattatgtac ttttctcaaa
    14701 ttattttcta tatcttctat ttcaattcta aatccattta atttaatctg aaaatctttt
    14761 cttccacaat aatatatatt tccatctaaa agatatccca tatctccagt tttataagct
    14821 ctccacttta caccatctat ttcatcatag aaaaatactt catcagtctt ttctttatta
    14881 ttgaagtaac ctttagaaac tgatggtcca attattataa tttcaccctt ttcattttct
    14941 ttaagctcat ttccatcttc atcaagtatt tttattttac aatttgacat agggtatcca
    15001 actggaagac ttttttcatc atctatagcc ttttgagtca tatcatttac acttacacca
    15061 acagtagcct cggttggtcc atatccattg ataattcttg tgtttggaaa tctactcata
    15121 agttctttag taagattttt tgaaagtgct tctcctatga atatcattga ctcaaggtta
    15181 ggaagcattt tactattaaa ctctttttca gttatacata ctcctgcaaa agaaggagtt
    15241 gagacccata cagctatatc tgagatacta aattgtctaa atagttctct atagtctgct
    15301 aatacatctt ttgatattga aaataatgta gctccatgga taagaccagg gtatattgtt
    15361 gtaactgaaa ggtcaaaaga atatgctggt tggttcatta ttactttttc acttccatct
    15421 atgtttagat atgggcttat ccaatctgaa aagctatcta aattgtttga acttatttga
    15481 accccttttg gctttcctgt acttcctgaa gtaaatagta tatatgcatt ttcatcatct
    15541 tttacccagt tttctttatc taatgatttt ccctgatatt catttattat atcatttaat
    15601 ttatccatat caattacatt gatatctcca aaatttcttt catcactaaa gttaaataat
    15661 acctttggtt ttatctcttt agtgacttca aaaactctat caattggaaa actaatatca
    15721 agaggcacat acgctctacc tgattttaaa gctcctatca tacatgccat tatcatgttt
    15781 tctttatttc cataaattac tataggagta tcttcttctt tgtatacatc ctttagaaat
    15841 actgatatag catctgaata ttcatttaaa tctttataag aaagtttatc tccattacac
    15901 attagtgctg ttctatctgt atttgaatat ttttttattc cttcaataat tttcatataa
    15961 gaatgctcct ttaaaatccg ttataaataa atggcaattc tttaaatgga gtaaatgtta
    16021 gaactcctat cgccaatatt ataaccatag ttaaacaaat actagttaac aaatctttat
    16081 tgttctttaa atattttaaa taatctctca cagacatcta cccatccttt cgtaccaaga
    16141 tgcattacat cccttaaata atacttatct gaccctttat ctttaaggtt tagaacttca
    16201 aagcctttgc tttctgctat tttttgcgct ttatcgtagt actgatttct ttctttttca
    16261 gaaataccag tcaaattata gaatttatcc atacttggga ttaatacaac aactggctta
    16321 atacctaagt cagtacatac atttagcatc agtttataat tttcaaactc cttagatgtt
    16381 aataaattta catctttgtc tcttcctttg tattgctcta taccatcttt aaaatgctct
    16441 ttataatagt acttatcaat atttagtgga tttttaccaa ctcttttttt agcatcttca
    16501 atggctttct ttctctcata tgaccaattt attggtcttt ttctttttgt tgctctcttt
    16561 ttatctagct taattaatcg tttataaaga ataccctttt ctttaagagc tatacaatac
    16621 tttcttcctt gaaaataagg ctctaaaagc accttttcag ctttttcagg aaatgtctta
    16681 ggctcatata gcttagcata taatgcttct gctttatact cgtcacttcc ccataataac
    16741 ttacttgatt tttttgcata ttcaatctta ttttgtttag atatttttgg attatctaaa
    16801 aatcgataaa attgtattgg tgaaaatcta gactgataat ggtgacttgt gactccgtct
    16861 ttttccataa accattgcat agaaattaac aagacaactt tcttattatc aatattagga
    16921 ttcatacttc ctaatattgc agcatcttgt aatgtctgag tataggctct cccaattgca
    16981 aacaccttat ttttactcct atttgtatta aaatagtact ttggatgttg ttttgttgaa
    17041 ttactaagtt ctgatgaccc caaaatcata atatcctttt ctcttaaaaa atgattattt
    17101 gctgtaaccc ctttatcttt tatatcactt aaagtatcat ccataattgg aagtagattt
    17161 ttctccctca aaagttcatc agttttagaa tcaagaaatt tgtctaaccc aaataaaaat
    17221 actacgccta ttataaaagg tgtaataaag tatatcaatt ttctcatatt ttctctcctc
    17281 taaaaatatt caaatttatg ttattattaa tcatattagt tttgatatgt atattcaact
    17341 acaaaattgt aataaaatcg tcaaaacttt ttttgatact gttacaaaat tgtaaaaaaa
    17401 aagaaaaaaa cgccaaaaat aaatttcttt ttagcacttt ttcatactta taatttgaca
    17461 ttttaacact tggttaagta tacaccttac cttaacttaa aattaaattt atttaagaat
    17521 atatattata aattcttata ttttctaata taaactatat actatcagct ggataaacaa
    17581 tactactttg tcttcttatc tcatccataa tttccattac tattattgaa ttttcatgag
    17641 aatttatatt tgattctatt ttattgttat ttataagact tataaactct acaagttcat
    17701 aatacattcc ttctatttct ctaggctttt cctctgcttt ttcttttgga atagatatat
    17761 cttcttctct tccatctctg tatttaataa tagctttttc aaatagatta agcttttcaa
    17821 ttattatact tccctcttct ccttgtattt cagatggtat ataagaatca gctactttag
    17881 aatactgaat tatcgcatcc atatcatcat attgaaatac agctgaacct tctccatcca
    17941 ctccagtttg aagtatatga gaaactgcct ttacagtttt tggagcacca aataaattca
    18001 ccataggatg tatacaatac actcctatat ccattaaagc tccatttgac aactcttttt
    18061 taaaggcatt ttctataatt ccatttttat atttatcata tcttgaagaa tactgacaat
    18121 agcttccaaa aaatcttctt attttcccta ttttatataa gttttcttta actgctttaa
    18181 agtttggaac acatgtaatt ctcatagctt ccataagcaa tacattatta tctttggctg
    18241 tttttatcat tgatttgact tcttttaaat tagatgctaa cgacttttca caaagcacat
    18301 gttttttgtg ttttaaacaa gtaatggttt gctttgaatg taatgcattt ggagaagcta
    18361 tgtatacagc atctatatcc ttcgatttag ccatttgttc taaatcatca aaaaaaatac
    18421 ttgctccata tagagttcca aattctctag ctttttctaa atttctagaa tatacagcaa
    18481 ccaattcaaa gtctttaacc ctagatgcag ctcttagaaa tatatttgct atattgcttg
    18541 tcccaacaat accaaatctt attttaccca tcataaatca ctccccattt tctacacttt
    18601 attttaattt taataaaaaa ctttacaact tatagatata ttataaaata attttatcaa
    18661 ataaaaaaga aagctaattt taaatgattt caccatttta acatagcttt cttttaacta
    18721 caatctaaat tcaacttata tttttaaagt tatctttcta ctatatcttt atttcccctc
    18781 aaaaatttta tagtaagaat ttcatccaca aacaatgtta ctaaaagtat agttacaaca
    18841 attggccaat aaggtattat aagactatat aaactatcaa ctatatacac ttgaaaatat
    18901 ctaactccaa taaatgtcaa tatagtccaa tatacagaaa actttagaca tactattcct
    18961 ctactattgt acttataatc tgaataatcc caatagacct tattaaaata tactttcata
    19021 agtattcctg taatatattc tactactgtc ggcactacta gacataataa aagcaatgca
    19081 aatatattat tggggtcaat aaatcttgag acctctatta taatcgacat tgcaattgca
    19141 tacattggtt taaatggacc atttaaaaat ccatcttctt gaaaatgccc ttgtttataa
    19201 taactatata agttttcaat tatccaacct ataaaaccat acaagaaaaa attaaataaa
    19261 aaatataata gagaacctac attatccatg aatattcctc ctagctgttt atacttattt
    19321 tttctcatat tcatggattt attctaaatc aaagaaatta tatttaattt atttaattaa
    19381 tactttatat tatagattgt gacattcttt agagttcaaa ctttttaggt attctctaac
    19441 ttccagtaaa ttttcaagta tcttatgtga ttgtgattta atttgctcat ctggctcttt
    19501 taaatctggt acatttatac atcttatacc accattgtat gcagcttcaa ctcccatagg
    19561 cgaatcctct atgacaatac aatttttggg attaacattt atctttttag ctgcttttaa
    19621 gaatatctca gggtttggtt tagagtttac aacatcatct ccacaaacta tagcatcaaa
    19681 gtaatctttt aaatttgcct tagctaatct ctttactgct ctttctctct ttgttgatgt
    19741 tgcaacagcc attttgtatc cattttcctt taaaaaagag attagttcat tgactcctaa
    19801 tttgatagga gctcccttcc tctccataaa ttcaatcata tttttagttt tttcgtcata
    19861 taaatctatt attggtacag aactatcgta tatatctgta agaccttcta ttattccttt
    19921 acgatttctt cccataactg atgtataaat ttccttagtc atagtatatc catacttttc
    19981 aaacgtctcc atccaaaatt caagagaaat tctctctgag tcaaacaaaa ctccatccat
    20041 gtcaaaaata attccttcta cattttgcat acaattcctc caatattttt tgatataatt
    20101 atattacact ttttatgatt aattttacaa ccctcaattt atccaattct aatattgtaa
    20161 aattgtaatt ttcaaaagtt acactttggt ttactttaat atttgaacca agcatatagg
    20221 atatccatcc acctatactg tcaaatccat catgctctat atcaagtcca aaatacttat
    20281 taatatagtt gatagattca gttccatcaa ctaagtaaga acctgctcct attttcttaa
    20341 tatgagtctc ttctttgtca aattcatctt gaatttctcc aactatctct tctagtatat
    20401 cttctattgt taccacacca gatgtaccac catattcatc cacaactata gcaagttgaa
    20461 gtttttcctt tcttattctc tcaagagctt tatctatagt taaattttca gatatatata
    20521 taatatctct caatatttct tctagttcaa ttttattttc atttatcttt tgattgtata
    20581 aatctcttat atgtacaaaa cctaatatat catctttatt ttttctgcat actggatatc
    20641 ttgtaacacc ttcctctttt aaaatagcta atatcttttc ttcagaatca ctttcatata
    20701 tacataccat atcagttcta ggaaccatta tctctctaat tttcttttct tcaaattcaa
    20761 aaatattatc aactaatctt tgctctgatt catctataag tccactttta taactttctt
    20821 ctactaatag tcttatttcg tcacctgtat ggggttcatc aacctcatca gcttgtgaat
    20881 atccaaatgg tttaagaaat aaatcagtac ttaagttgaa aaaatatatt attggatatg
    20941 ttaacttata aaatcctact aataaaaaag atgtactcaa cattattttt tctgtattat
    21001 aaagtgctaa agctttagga accaactctc ctattacaac ctccatcatt gttataatta
    21061 aaaaagaaat ggaaattgat atagtatata taatactttc actaagatta aatacactta
    21121 ctattggtaa aattaactct ttaactgtag attctcccat ccaacctaat gcaagtgaac
    21181 aaagtgttat accaagttga catgcagaaa gataggaatt taagttgtct tttattattt
    21241 tacatctttt tgcattatta ttaccttcag ttatcatggt atcaatccta gaactacgaa
    21301 gttttaccat agcaaattca caagctacaa aaaaagcatt tatcaaaaca agcattacta
    21361 taagtgttac ttttaataac tcccgcataa agtattggtt cttataatgt aagaaat
//
LOCUS       DAELNY010000052        21266 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.052, whole genome shotgun sequence.
ACCESSION   DAELNY010000052 DAELNY010000000
VERSION     DAELNY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.052"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(213..1604)
                     /locus_tag="KPE55_001663"
     CDS             complement(213..1604)
                     /locus_tag="KPE55_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0492749.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1615..2835)
                     /locus_tag="KPE55_001664"
     CDS             complement(1615..2835)
                     /locus_tag="KPE55_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG0492750.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2849..3637)
                     /locus_tag="KPE55_001665"
     CDS             complement(2849..3637)
                     /locus_tag="KPE55_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0492751.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3654..4928)
                     /gene="larA"
                     /locus_tag="KPE55_001666"
     CDS             complement(3654..4928)
                     /gene="larA"
                     /locus_tag="KPE55_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG0492752.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5046..5759)
                     /locus_tag="KPE55_001667"
     CDS             complement(5046..5759)
                     /locus_tag="KPE55_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0492753.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5884..7143
                     /locus_tag="KPE55_001668"
     CDS             5884..7143
                     /locus_tag="KPE55_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG0492754.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7423..7989
                     /locus_tag="KPE55_001669"
     CDS             7423..7989
                     /locus_tag="KPE55_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0492755.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8190..9395)
                     /locus_tag="KPE55_001670"
     CDS             complement(8190..9395)
                     /locus_tag="KPE55_001670"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0492756.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9498..9604)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9696..10064)
                     /locus_tag="KPE55_001671"
     CDS             complement(9696..10064)
                     /locus_tag="KPE55_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG0492757.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10116..11393)
                     /gene="obgE"
                     /locus_tag="KPE55_001672"
     CDS             complement(10116..11393)
                     /gene="obgE"
                     /locus_tag="KPE55_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG0492758.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11504..11794)
                     /gene="rpmA"
                     /locus_tag="KPE55_001673"
     CDS             complement(11504..11794)
                     /gene="rpmA"
                     /locus_tag="KPE55_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG0492759.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11808..12125)
                     /locus_tag="KPE55_001674"
     CDS             complement(11808..12125)
                     /locus_tag="KPE55_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG0492760.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12140..12451)
                     /gene="rplU"
                     /locus_tag="KPE55_001675"
     CDS             complement(12140..12451)
                     /gene="rplU"
                     /locus_tag="KPE55_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG0492761.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12552..13973)
                     /locus_tag="KPE55_001676"
     CDS             complement(12552..13973)
                     /locus_tag="KPE55_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG0492762.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13991..14692)
                     /locus_tag="KPE55_001677"
     CDS             complement(13991..14692)
                     /locus_tag="KPE55_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG0492763.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14694..16538)
                     /locus_tag="KPE55_001678"
     CDS             complement(14694..16538)
                     /locus_tag="KPE55_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG0492764.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16857..18050
                     /locus_tag="KPE55_001679"
     CDS             16857..18050
                     /locus_tag="KPE55_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG0492765.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18262..19344)
                     /locus_tag="KPE55_001680"
     CDS             complement(18262..19344)
                     /locus_tag="KPE55_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0492766.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(19908..20699)
                     /locus_tag="KPE55_001681"
     CDS             complement(19908..20699)
                     /locus_tag="KPE55_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492767.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20703..21032)
                     /locus_tag="KPE55_001682"
     CDS             complement(20703..21032)
                     /locus_tag="KPE55_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492768.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6715 a         3828 c         2361 g         8362 t
ORIGIN      
        1 tataagatac aatagtctta ctttatttat gtacacatgc ttttaggatg acaacatcaa
       61 cttaaatatt atgttacttt ctaaatttaa gtaaatattg ccatcctaaa aattttacaa
      121 tttagcttca gatttaaaag taaagtttaa aatattatat ttaaatctaa tttaatattt
      181 tttgttttat aaatattatt aaattaatat ttctaatata ttttccctgg atttaaaata
      241 tttttagggt caaaagctaa ctttatattt ttaattaaca tcatataagc atctgaattt
      301 gattcatgta agtactcttt tttagcatat cctatgccat gttcaccaga aacttgacca
      361 ctaagttctc tagatttttt atacatatag tcaaaagttt cttttaatct tattttccat
      421 gtattatcat ccataccatc ttttaaaata tatatgtgta gatttccatc tcctgcatga
      481 ccaaaacttt ttattcttat ttttaatttg tcttgaagct cgtgagtgta tcttataaac
      541 tctgctatct tatctcttgg aacaacgaca tcacattcat ccatttgtgt cgtagatgcc
      601 tttatagctt ctaaaaatgc acctctggct gaccatatag aatcgtttct ttcttgtgta
      661 tctgatataa atacatctaa tgcaccattt tctaaacata aattagctac tttttcatat
      721 tctttttcta tatcttcagt gctatttcca tcaaatgtaa gcaaaagata agcatctgat
      781 gtattatctg gaaacttttt acctaaaaat tcttctgctg ccaatatcac atctctttcc
      841 ataaattcta tagccgttgg tattgatttt gattttatta ttttaggaac agtttctatt
      901 gccattgata aatctggaaa tggtataagc aaacttatac ttttctttgg taaaggaagc
      961 aattttaata tagctttagt aactattcct aaagtacctt ctgagcccac taataaatcc
     1021 tttatactat atccagaact atttttaacc acctttccac ctacatttat aatttctcca
     1081 tttggtagta caacttcaag tcctcttaca taatcccttg taacgccata ttttactgct
     1141 ctcataccac ctgcattagt gtttatatta cctgcaattg tagcactttt ttcacctggg
     1201 tcaggtgggt aaaataaatc tctatcttga acatattgac ctattgtcat caataaaaca
     1261 cctggttcta ctgtcaatgt taaattttca taatcaactt caagtatttt attcatttta
     1321 catagattaa tcattattcc accatttatg gcaacagcag cacctacaag accagtacct
     1381 tgacctcttg gtgtgactgg tatattattt ttatatgcgt atttcattat tttagatact
     1441 tgttcagttt caagtacatt tactaaaact tcagggtact tctcaatccc tccaagttca
     1501 tcatgtgaaa aatcttcatt tatatcactt ccaactaata cattttcttc tccacaagta
     1561 tctattaaaa aatctatatc ttttttatcg attaatttat acatttctat ccccctattt
     1621 agctgctgat gccatatatt tttgattatt atctttgtta ttttctattt tttcaatcaa
     1681 tttaggaata atatcataaa tatcacctat taaagctaaa tgtgctacat caagtatagg
     1741 cgctttatca tctttattta ttgctactat atagtcagca ccctgcatcc ctgcaacaaa
     1801 ttgaactgct ccagatacac cacaagttat tatcaattta ggttttacag ttctaccact
     1861 aagacctatt tgttttttag catctatcca acctgcttct attacaggtc ttgttcctgc
     1921 cacttgagcg tttaacttat cagctaattt atatatcatc tccatatctt cttgtttttt
     1981 tattgctcta cttgccacaa ctatcacttc agcttcttcc agaccaactt ctttattttt
     2041 ttcttttaca ctcagtacct taattttaga ttttagttct tttttttcta ctttacataa
     2101 agttatcttt cctgtagtat tctctatttt ttctggtgct gaaaatattt tatatcttac
     2161 agtggcaaat tgtggtctat tattaggtgt gtttatatga gccattatat ttccaccaaa
     2221 tgctggtctt atttggtcta aatcagtatt tgattgaata tcaagtattg tacaatctgc
     2281 tgtaagacct gttctaaatc tagcggcaag tcttggagct aatgacctac ctaatgtagt
     2341 tccaccaact aatactatcg ttggttttat tttatctata aagttttcta ttgcttttga
     2401 atatggttct atcctaaaat ctttaaattc atcttcatca tatacaaata cttcatccac
     2461 cccataagaa atcaaattac tgcacttatc ttttatgtct tttcctacaa atacacaata
     2521 cacaggttgt tttatttttg atgccatctc acgagcttta cctataagct cgtacgttac
     2581 aggatgtata tttccattat gatgttcaac atatactgca attcccctcc attcatcttt
     2641 attaatttgt actttactac tctctataaa ttcaaatgcg ccttttggtc cacttttgac
     2701 acacaacttg cacatcttac agccagcact tatttctact atacctgact tttcttctat
     2761 agccccaaaa ggacatattt ctaaaattct ctttaagtca gttattttat cttgatttac
     2821 aactatcttt gacatttatt actccccctt acaccaactt taattcctct aatttattta
     2881 caagtgcaaa acttagttca tctgcatttc ctcttacaat atttttatct gtatttggct
     2941 ttggaggaaa tattcttaca acttgagtag gagaaccatt aagtccatag ttattttcat
     3001 ctttatcttc aaagtcatct aagctatata caggtatttc tctatcttta gtattaagtt
     3061 ttaatttaaa tgaaggtagt cttggttgaa aaatgtcttt ttctacagtt attaaacatg
     3121 gatattcaat ctcacatact tgtaaatcat ttggcatatc tatttcaaca actatacttt
     3181 catctttaac ttcaattaat tttgtaacgt tagtaacatg aggtatatct aaaaattcag
     3241 ctacttcagg tcccacctgt gctgtatcac catcagtagt ttgttttcca caaattatta
     3301 ggtctacctt tcccatcttc ttaatacctt gtgatatggt ataggaagta gccaatacat
     3361 ctgctcctcc aaatctccta tcacttatta atgctccttc atcagctccc atcataaaac
     3421 tttcttcaat taccttcttt gcctgaggag gacccatgct aagtactttt agattcgctt
     3481 tcttatcttc ctttattcta agagctgttt ctaaagcata taggtcatag ggattcattt
     3541 ttgaatctac tccatctctt tttagtactc ctgtagtttc atcaacttct actgaagtcg
     3601 ttccaggaac ttgtttaata catgtcaaaa tttccataaa gtacactcct tttttattta
     3661 actataactg atactccatc aggtataact actactttgc tctccttacc aactatacta
     3721 ttcgccattt ctaaagcttc tttaaagtta aatgcatgtt tcatatgcat tttttttatc
     3781 atttcaggat tacacttatc tgttaccatt atcacagtat atttagataa tattctcgct
     3841 agtatctgaa attcccattg gtctggaatc gtatctactc tatcgacact tctaatttta
     3901 tctaaaagtt gttttggaga atctgcattt gctacatttt cataaaaaga ttgacctcca
     3961 tgaccatcat tacatgctga aatcattatt attactccac cgtctttaca agtagcttca
     4021 gctgctgtca ttcctttaac agattgataa atattttggt ctagtggata tccaccattt
     4081 gtagttacaa caatatcaga ttttatagaa tttacttttg ataactctgt tacaaatcta
     4141 catcctttag tatgtgcttt ttcgacatca ccagcaaagg aagcaattat ttttttatct
     4201 ttatctatta caacattcaa tatgaatgct aattttgcaa ctttagctgc atataccata
     4261 tcctcatgaa taggattatt attcaatttt ccagttcttg aattatcact atctataaag
     4321 tcactacaat gattgtacat tatagtcttt gcagatgcga ttcctggaag tacacttttt
     4381 ctgccacctg aaaaacctgc aaagaaatga ggttctataa aaccttctgc tatcaaaagt
     4441 tctgcctcat atgctagttt gtttatatat aaatctccac ctgaaggcaa tatccctact
     4501 ttacacatag atttatcatc agtagatata tgcataacta tatcttcatt atctactatt
     4561 tcttctccca ttttataaat tagctcttcc cttgtacttg gcctatgaaa tccagttgcc
     4621 actataatct tcacatcaat ttctggatta acattcctaa tccttcttaa tataattggc
     4681 atagttattc tacttggtac aggtcttgta tggtcacttg taattatgac tacattcttt
     4741 ttccccttaa ccagttcttc cagagaatta gagcctatag gattattcat agattcttca
     4801 actatctctt cttgagtctt atgttctata taattatccg attttgactc caaaacaccg
     4861 agaaaacgtt catcaggtat ttctaaaatc attccttgtt ttgaatacgg tacttttaac
     4921 tttaccattt ttaatctccc attataataa actataaaat tggtatgtgg tcataccagt
     4981 tttacattta tatgatagta ccattatcta ccatttgcaa gaataattta ttttataaca
     5041 aacttttaat tttcattttt tctataattt ttacttataa aatccatatg ttcctttgct
     5101 ttctcacatg caagttctac atcattttct acaactgcat tgtatatagc ttcatgctgt
     5161 ttaagcaata tattttcatt aaaaggttct gcgattattt tttctcttgc atcttctata
     5221 aatccatcaa ataattttga tgctgtcata aaaagacttt gaattaaata atttttactt
     5281 aatgttgcta gtttactgtg aaatttttgg tctaataaca ccagtactcg attattatct
     5341 tttttattag tttctaccct catttcatct attatatgtt tcaattctat aacttcagaa
     5401 gaagttattc tctttgaagc aagttttgca atctcaattt ctaggattat tctaagctct
     5461 aatatatctt caagtgtacc attattaagt ttaaaaatca tagataaagg ttctattaaa
     5521 gttttttcta tatttgtaca tataaaattt ccttcaccct gtctgctttc tatcactccc
     5581 atagtctcta ataccctaat agcttccctt atggaagttc tactcacatt catttgttca
     5641 gacagttctc tttctgatag taatttatcc ccctttttta actctccatt tagtatttta
     5701 tattgtattt gctcaattac ttgttcataa acttttttac tagctatact attaaacatt
     5761 ttttcctcca atatgataat cgttgttgta atatatatct attatatatc ttaagccatt
     5821 acattcatat atttttatta cttttaatat aatatcttat aactcaaatt tacaggtgat
     5881 attatgaaaa aaaataaaat tattgctttt tttgctccat ctatcatagt tggagttcta
     5941 gttttaggga ttatcctata cccaaatgaa gctataaaat ctgcaagaga aggcttttct
     6001 atatggtcta atgttttagt tccatcacta ctaccattta taattggagc aaacttaatt
     6061 gttgatttaa aaatagtgga tattataggg tttatcatca atcccataac tagatttgta
     6121 tttaatgttt caggaaagag cgctttagct tttgctatat ctacagtatc tggttatcca
     6181 gtaggagtaa gtttagcttc tgaatttagg agtaatggac aaatttctaa gtatgaagca
     6241 caaagacttg tgtctttttg ttctacatca ggacctttat tcataatagg ttcggttggt
     6301 acaggtatgt tccaaaattc aaacttaggt tatttaatga ttctctgcca ttatctagga
     6361 actatattgg ttggtctttt ctttagaaat tatggtaatg aaaatcttcc aaagacaaaa
     6421 ttggatttaa aaacaaatat aaaaaatgta atcaacatta gaaattcaag tgggaatggt
     6481 ttttttgttt tatttggaaa tgctgtattt aatggagtaa atacattact tacagttggt
     6541 ggatttgtaa ttgttttttc agtagtattt aaaatactat ctctttttaa tgtaatctca
     6601 ttaatagcat cagttatata tttgcctctt tcatttttag gtgttagtaa agaactctgt
     6661 caagcatttg taagtggtct atttgaaatt actataggat gcaataaagt atctagtata
     6721 acaagttctc cagaaatttt aagagcatct ttagctagtt ttttgatagg atttagtgga
     6781 ttatccatcc tagctcaatg ttgtacattt ctatcaaaaa cggacataga tttaaaatta
     6841 tacatactaa gtaaatttgc gcatggagtt ttagctgcta tctttatatt tatactttat
     6901 ccaattgcaa actctgctgt gttggtttct agctttgctg atacatacaa cgttatatat
     6961 aataatttaa tctggtttta ttatcttagc tattatgata caattcttca aattgtagta
     7021 ataatttatc taatctcagc cgtatttatt gcaaaaaaac atatgaaaac tttatctaca
     7081 aatgggaaac taatgcgata taaaaatact tttttcacta agaaattttc acgaaaaaaa
     7141 taaaactaaa aataaaacta ataaaactta actaaatact tccatttttt tattatattt
     7201 gtcataattt taataacctt tttaattagt ttaacagcat agttatttca tcacaattta
     7261 ataattttat tagaaatatt tttcaaaaaa ttctccttaa tcacatatat cacacttgta
     7321 taagtttact gggaaatttt tccattatat aatatttcat ttatattata taataaatac
     7381 cgacctaaac ttaccaaatc aaactaagag gagtggttga aaatggcaac aagacctata
     7441 gaaatagacg aatataaaaa gataatggaa ctattacata ctggctttac ttatagtgaa
     7501 aatggcgttg aaaaaagatt tagaaaaaat cctaaagttg cattggcact aatgttagaa
     7561 gctaatttag gtctaagaat atcagatatt ttaagattaa agataggtaa ttttaaaggt
     7621 aatatgttgg aaataaatga aaaaaagacg ggcaaattac agtatagacc agttaataaa
     7681 aatataattg agtccataaa tgaacatgca agaaagtata atcttaaatc aaatgactac
     7741 ttagtaaata taaagacaaa ggctattcaa aaacagttga ggataatttg taaatactta
     7801 aatctgtata atataagtac acatagcttt agaaagctct atgctactac tcaatttgaa
     7861 aagagtaaca ataatttaga gttggttaaa gaacttttaa atcacagctc tgtagccact
     7921 acacagcgat atataagggt aactcagcaa gctattaatg aagctagtga aaatttcttt
     7981 attggttaat tataacgtat acatttttct attatcttat taattcacca gatattaaaa
     8041 aagagtgtct caaaatgaac tttttagttc atgagacact ctttttttat aaaatcaaat
     8101 atatttttat tatttcaaca ctggaaaaaa ggcttatctc tctattttga gacaccctat
     8161 taaattttca taattcttgc ataaacatat taatctatct tcaataattt tttagctgat
     8221 tcactgtaat tatttctttt tacaatttcc aaaactctat caatctcatg ttctgacttt
     8281 gcacttacaa aaatcgccaa ttcttcatca tctataatat cataactatc tacatattta
     8341 tctaaacatt ctaaaattcc atctttatta ttatacccac caaatctaac tactgcacta
     8401 cactcatctt ctataagtcc acttaaatcc aatttccctt ttgaaaaaga ctttatattt
     8461 gaacttctat tatctattat atctataata tctgcataaa cagcacttcc tgttgcacct
     8521 cttccagctc ccttacctac aaaagaaagt tcacctactg aatcacctac taaaataact
     8581 acgttgaact cattatctat ctttgaaagc atattattat tgtccactaa tgctggtcta
     8641 acaaatccac ttacttctcc tgattcatac ttacttctag caaccaattt tatcttacaa
     8701 tttaattttt ttgcatattc tatatctttt tcatctattg aagttattcc ttctaataat
     8761 aaatctttcc aatatactat ttttccataa gataaagtag acaatataga taactttcta
     8821 gcactatcat atcctaaaac atctgcatct ggattaactt ctgcaaatcc caactcttgt
     8881 gcctgtttta aagcgacatc atacggtaaa ttttcatcat acatctttga taatataaaa
     8941 ttacaagtcc catttaaaat agcgtttatg ctctttatat tgttgccttc cagtgattct
     9001 atcaatggtt ttaaaactgg aataccacct gccacagatg cttcaaactt gatgctaaca
     9061 ttatttaagt tagctaattc tataaggtca ctaccttttt cagccagtaa atctttatta
     9121 gctgtcacta catgtatttt tttatttaac gctcttgtta cataatcaaa agctggattt
     9181 aatcctccaa ttacttcaac tagtatatca atatccttgt caaatatatc ttctatatca
     9241 gtagttatct tatcaaagta cttcttatac ttgtgttttt ctttattttt aactaaaatt
     9301 ccaacaactt ctatatctct ttttgaagta ttattttcta ttaaatcaat taatccactt
     9361 cctactgttc cataccctaa tattgctatc ttcactgtaa cataccccca tcattttgat
     9421 tatattaaat tatttatata tataaatata ccaataaaaa aagcttgagt ataaactcaa
     9481 gccatatgac gggtttatac tcatctttca gcataaatgc tgcaggattt agcaccaatg
     9541 tataattaaa tatactggtt gccgggcttc acagggccag tccctctgcc actcttgata
     9601 agattgctaa tttaaattta tgtttaatag tataatacaa tttcttccaa ttgtcactat
     9661 ttttttaact tatttcccta aaaggatata aactattatc taacagctct tttagttggt
     9721 ttaccctttt ttgtaacatt attattttta gtctttgctt tcgcctgttc atgtcctgga
     9781 aaaagtattt taggctcttc aatattcctc ttgtaaatag taaatttaaa accaatagcc
     9841 tgcacaaatt cagctcttaa agcttcacat actgcattag cagtttcttt agtatcaaga
     9901 cctgcatttt ctaatatagt aacttttact aattctctag ttcttaacat accatctaat
     9961 tgctctaaga aactctctgt aacaccttct ttaccaattt gtgtagtagg ctttaaagta
    10021 tttgccaaag atcttaaata cgccctttgt tttcctttta acatattctc acctctataa
    10081 ttctatactt aaattatcta taatctatat ttaaattaat tgtaaaactc aaattctaat
    10141 tcataaattt taaccacatc tccatcttct attcccattt ctctaagcct atcaaaaaca
    10201 ccttgactct ccatagcttt ttggaaatat tgaagggatt ccatatcatc aaaatttaca
    10261 gaatacatta ttcttctaag tgctttacct gtaactacat acacaccatc ttcaatatct
    10321 atacttaact cttcttcagt accaatatca agttctggtc tatacatttc ttcttctgaa
    10381 actagctcca catcttctac ttcttttagc attttagaaa cataggctat tacttcatct
    10441 atgccttgac gagttgctgc tgacatttta aatactgtat atcctctgcc ttctaattca
    10501 ctcttaaact tctcaaactt actctcatct tctaatatat caaatttatt tgcaactaca
    10561 acttgtggtc tctttgaaag cttttcattg tacaatttaa gctcatcatt tatcttgtca
    10621 aaatcttcaa tagggtctct tccctctaat ccagatatat ctacaatatg tatcaatacc
    10681 tttgttctct caacatgtct taagaactca tgacctaatc ctatgccttc agaagcaccc
    10741 tctataattc caggtatatc tgcaagtaca aaactatctc caaacttagt ttgaacaaca
    10801 cctagatttg gtgtaagtgt tgtaaaatga tagtttgcaa ttttgggttt agctttagtt
    10861 acaactgata gaaatgttga tttacctaca tttggaaatc ctaaaagacc aacatctgct
    10921 atcattttaa gttcaagagt tatccatctt tcttctccat ctgtaccaga ttttgcaaaa
    10981 gctggagctt gtcttactgc atttgcaaag tgttggttac ctttaccacc tataccacct
    11041 tttgcaacta tagctgtatc tccttctttt tttaaatctg ctaatacaag acctgtagcc
    11101 tcatctctta ttactgtacc tgctggtact tttaatacta aattttctcc atttttacca
    11161 gctctttttc ctttgcttcc gtcgccacca ttttgagctt gatatttttt ctggtactta
    11221 aagtccatta gagttctaag ccccaaatca acttcaaata tgatgcttgc tccacgacca
    11281 ccatctccac cgtctggacc accagctgga acgtattttt ctcttctaaa tgctaccgaa
    11341 ccatttccac catttccagc cttaacaaat attcttgcct tatctataaa caaattcttc
    11401 acctacttaa ttaaaaactt ttaattagca ttaccaaaaa taccattata aattcatcag
    11461 tattataaga aaaaggagtg cctcaacact ccttttatgt atattactca gctatagcta
    11521 ctggatatat actaactttt tttctcttct tgtcttttct ttcgaattta acagtaccat
    11581 caactaatgc aaataaagta tcatcactac cttttccaac attagttcct ggatgtattt
    11641 tagttcctct ttgtctaact attatacttc cagcagttac taattgacca tcgaatctct
    11701 taacacctaa tcttttagat atagaatctc taccattttt agaagaacct accccttttt
    11761 tagatgctaa taattgtaaa ttcatgttta acatatggag tcacccccta cttttttaaa
    11821 atttttatat tttttgggta ttcccgttta atttctttaa ccgctaattg aaagtgattt
    11881 agtaataatt gtgcgtcttt tacatgtgta tctttgacta tacactctat atgaccttct
    11941 tctcctacta ctttttcaaa ttcaatcttt tgtaatttgt ctaaagaatt tataactgca
    12001 atagcattag atgtaactgc agcacatact atatcatagc ctatttcagc aaaatcagca
    12061 tgtcccttta aacaaaaacc ttgcaacaag aaatcatcat tataataata tttaactttt
    12121 atcataactt aaccaataac taagcgttta ttttttctac aactatctta gtgtatgatt
    12181 gacgatgtcc ttgttttctt ctgtaatctt tttttgcttt atacttgaaa actataactt
    12241 tcttagcttt accttgttca acaactttag cttgaacaga tgctccttct actactggag
    12301 aacctaattt taattctcca tctttagagc aagctaatac ttcgtttaaa gtaacaacat
    12361 cacctgcgtt agcttctaat ttttcaacga ataatacatc accttcagaa actttatatt
    12421 gctttccacc agtctttact atagcgtaca ttaaatacac ctcctcggcc tcaaactcgc
    12481 cagtacaagg tacttcattt gaagatttaa acctttactg tgcggcatta caattattaa
    12541 ttataaaata tttacatttt attgtcaact attttatcga aaataacatt ggttttattg
    12601 tgctttaatg tactatttgc gtttaaagaa atctttatat tatatttttc accaatttta
    12661 tcaattatat ccatataata ttcttttatt tgttcaaaaa taaaatcatt aaattctacc
    12721 attatgtttt tgtaaacagt atgttcactt attctcataa tttctttttc aatactatct
    12781 agtatataat gtacagattt tattgtttgt tcaccatcac aacatggaca agacattaaa
    12841 taatacttat caatggattc tttttctcta cgcctagcaa cttctactaa accaagtttt
    12901 gtcataccta atacttcagc ttttctttta tctttattaa attcttcttc taggatgtgt
    12961 aaaatctcct tttgatgctt ctttttatgc atatcaataa aatcgattat tattatccca
    13021 gctatgtctc taattctaag ctgtcttaca atttcttttg cagcttcaag attagtttta
    13081 taaacggttt catcaagttt accagttcca ataaacttac ccgtatttac atctataacc
    13141 gtaagagctt ctgttttttc aattattaaa taacctccac tctttaacca tactttttta
    13201 tttaataatt tttctatttt agattctatt ctgtataaat caaatacatc tctattttcc
    13261 tcaagacaca gtttatcaac ataagtctta tctatacctc ttaaaataga cttaagttca
    13321 gagtacttat cataactatt ggtaattata gattcaatat catcattcac attatctttt
    13381 acatatttag ttgcaaaatc taaagactta tataataatt tcggacctat accaagttta
    13441 tattctttta aaatgttttc atactttaat ttcaactctt ctatatcttt ctttatttca
    13501 tcatgagtgc aaccttgagc ctcagtcctt attattaatc ctagattttc cttatttact
    13561 gccttagtaa ttttttttaa tctaaatcgt tctttttcat cagtaatttt atttgatatt
    13621 gtagttctat cattggaagg tatatagaca atataccttc cagaaatact aatctcagtg
    13681 tttaattttg cgcctttagt tcctatttct tctttattta tctgaaccaa aatatcctga
    13741 ccagatttta tagtacttcc cttttttaat tgtaaataag caagtttctt aaatccaata
    13801 tctacaaaac aagcttctat tcctggtata acttttttta cgattccacg atatatattg
    13861 gatacagtct tcttatttaa attgtcttca acaaaaagtt ctgttaatct ttcatcttca
    13921 agtactgctg ttttctggga ccctattagg gactcaataa ctatcttttt cacaacttca
    13981 cctctcgagc ttacatagga gtcactaatt ccccatcttc taatacatat aaatctcttc
    14041 tcaatatatc aacatctaat gggtcaatat caagttcaaa catctctaat atctttggta
    14101 taagtatatt agtatttaaa ttagtttttg agcctgtagc tatagtagca gtaagagtaa
    14161 tatgttcatc ttctaaattt aaaacatcaa aggttcttat cataggtctt atatctactt
    14221 caaccatctt acctttttta ttcttttttg ttatgattat ttctttatta ttcataaagt
    14281 caattacttt tctttttaca aactctttcg tcataggtct tttcaaatct atattaaaca
    14341 aatattctcc atagtctatc acagaagaaa gagaaggtgt ttgtctatca atttcttttg
    14401 ctttgataaa atctatccca tctggaagtt gaattgatac tttatttaaa aattcctcta
    14461 ctgataaatc atcttcttct atttcaatat ctacatactc accttgactt tctgtcccta
    14521 aagccaaagc attgccataa ctcattttag ggtgaggatt aaatccttga gaatgtgata
    14581 agtttatttc agctcttcta aacgctcttt gtaacaatct ctgaaggtca agatgtgata
    14641 tatatatcat gtcgccttcc tttttaaatt ttactcttat tatcttactc atattagcaa
    14701 agcccccttc ctatatcatg tatatttatt ccacaaccat tgcagttatg tctacaatca
    14761 gaagtgattt tttctttttt agcattttcg tcttctctaa ttaagaattt tttactaata
    14821 cctacatcta tatggtccca aggaaatacc tcttcatagt ttctatttct atgtgcataa
    14881 aaatctatag aaagattatt cttttccata gcttctttcc atatatctaa attaaaatgt
    14941 tctgcccatc catcaaattt agcacctagt ctaaatgcat catatataac ttttcctatt
    15001 cttctatcgc ctctagctac tactgcttcc atcaaactag tctttgaatc atgataattg
    15061 tattttatat tgttatttct taatttattt actaaatgtc tctgcttatt aataacttct
    15121 tctgtagtat cttgaccatg ccattggaaa ggtgtaaatg gctttggtac aaatgtagaa
    15181 gtactcactg taacactaaa acttctcttt agctttcctc catttacttt tctataaata
    15241 tcaataactt tataagctaa tttagctata ccatctaaat cttcataagt ctcagttggt
    15301 aaaccaatca taaaatacaa cttaacacta ttccacccca tctcaaaagc tctttctgtt
    15361 gcattttcca aatcttcttc tgtaacacct ttatttataa catctctaag tctttgagtt
    15421 cctgcttctg gagcaaaagt aagtccagat tttcttactt gttgtatttt atcagcaatt
    15481 tccattgaaa aattatctaa tctaagggat ggaagcgaaa tcccaatatt attagatgca
    15541 tattcatcta ccaaataatc agtcaactct gataacttac tataatcact cgtactaagt
    15601 gatgataatg atatttcatc atatccagta ttcttaacta atttatctaa tatttcttta
    15661 agtcttttaa cactcttttc tcttattggt ctgtatatca tacctgcttg acagaatcta
    15721 catcctctag tacatcctct aaataattct agtactattc tatcatgaac agtatctata
    15781 taaggtacta ttagtttttc tggataatct acagtctcaa catcttttat tatcctttta
    15841 gtaggatttt tagtaatccc ttctctatta ggagttacac tttgaactgt accatcttca
    15901 ttgtacttga catcataaaa acttggtatg tatactcctt ctatgctaga tattttatat
    15961 aaaaaatctt ctctagtcgt tttatttttc ttccattctt tatactcatt tactacttct
    16021 aaattaacct cttcaccttc acctaatatt actacatcta caaaatctgc taatggttcg
    16081 gaattataag cacaaggacc accaaccatt ataaatgggt cttccaaagt tctttcttct
    16141 tttaatattg gtatattggc taaatctaat atatttaaga tattagtgta tgaaagttca
    16201 tattgaagtg taaaagtgac aaaatcaaag tttgttatag gctctcttga ctctaatgca
    16261 aataatggta tacttttttc tctcattata ttttccatat caactgcagg agaatacact
    16321 ctctcacaaa atacatcttc atccttattt attacatcat ataatatatg actccctaag
    16381 tgactcatac caacttcata taaatctgga aagcaatgtg catatcttat caattcacta
    16441 ttactagtat ctttatgaat tgaatttatt tcatttccaa gatatctagc aggcttttct
    16501 acttttttta agattttttt caagtctact ttattcattt attaaaattc ctccaaacaa
    16561 gctctttatt tttattctta tttataagta atattataca cgataaaata ttttctatca
    16621 aaaatataac taaaatattg aatttaaaat acatattatt cacaaaatag aatagctaaa
    16681 aatatttaag aatattttct attaaatttt tattgaaata caattttttt acaaagtatg
    16741 atatattata aacataaaac tattgtttta catgacttat catgacatct tttgtttcat
    16801 tcacctcaaa atggggtatg ttacttatat aatgataaat tttaggaggg ttaaaaatga
    16861 gtaaagtttt tgaagtaaaa aaagatatct actttactgg agtagtagat gaaggtttaa
    16921 aagtattcga cataataatg gaaactgagt ttgggacaac ttacaactct tatttaatca
    16981 aggacgaaaa aacggtttta tttgacactg taaaggcaaa ttttaaagat gagtttttaa
    17041 gtaatttatc agaggttact gatatagcta aaattgacta tgttgtaatt catcacacag
    17101 aaccagacca tgcaggtagt ttaaaatact tattagatat aaatcctaat atagaagttt
    17161 attgtactaa agctgctaaa ctatacttag atggtcaaat aaatagacct tttaactgtc
    17221 atgtaataaa agatggtgaa attttaaata tcggtaaaag aaatcttaga tttataactg
    17281 ctccattctt acattgggtt gatacaatgt ttacttacat tgaggaggac aagactcttt
    17341 taacttgtga tgcatttggt tgtcactttg caagtgttga tgcagaggta gtgaatagtg
    17401 aagattatct aaaatcagct aaacactact atgattgtat agttaagcca tttgctaaac
    17461 atgttttaag tgctgtagat aaggtggtag gactaaacat agaatttgat actatattga
    17521 cttctcatgg tccaatgctt actaaagacc caatggctgc tgttaaaaga tatgttgagt
    17581 ggtctacaga agctgttaat acaactaatc aaaatcaagt ttctatattc tacttatcag
    17641 cttatagtaa tactttagag atggctaaaa agataaaaga aggacttgat aaagaaggtg
    17701 caaaggctga attatatgat ttagaagata tgacattaac agaaatgcac gatacattag
    17761 tagtgtcaaa agtaatatta ctaggttctc caactataaa caaaactatg gttaaaccta
    17821 tgtgggattt attctctgtt atagacccaa tggctaacca aggcaagata gcaggagtat
    17881 ttggttcatt tggttggagt ggtgaaggta taactatggc tgagacttta cttaaatcta
    17941 tgtcattcaa aatgcctgta gaatctctta agaaaaaatt cttccctagt gaagagacat
    18001 taaaagagtg tatggcattt ggtgcagaat ttgcaaaatt agttaaatag ccattaatca
    18061 aacataatat tatttaaaat aaatgatttt atatttaaaa ataaattaaa atggagttgt
    18121 ctcaaaatta atttttctaa tctaatttta agttcaactc cttttttatt ttctaaaatg
    18181 actgatataa tgataaatgc accctttgtg cgagggtgca ttatttataa tatgttctat
    18241 ttgtacacta gtatatttgt attaattcac atatctagcg tatgctacca tccctcttcc
    18301 agaattagat gccgtttcac caatttccac tttaataatt acattatttt ctttacactt
    18361 ttttgataat tctgctgcag aaaggttctt accagtaaaa tctaaagaaa atggcttatc
    18421 atattcaaaa ggatactcat caggtacacc atctggagct aatgcaacca taccatatgg
    18481 tgtattcata aaccctatta cagtaactgt atgctttgat gggtctaaat ttgaattttt
    18541 gcttgtaaac ttaattgtat ttattgatga gttgattaaa ctacacggaa ctccctcata
    18601 catttcaatt tttggacgta gattatttcc ataaacttca tcaagtattg ccccttgttg
    18661 atctgctatc atagtgtgat tcccaggtcc aactggcgca gaaggcttag ctggcgtagg
    18721 tttcttaaca gttggcgttg gattctgtgt ctttactact tcgccaacag ctggatatgc
    18781 tttcttgtca aatggtaatg ggtgagtaag actcttgtat atgatgtctg ctcctgctga
    18841 tttgttggca tcaatatcta catgatacaa acgtccatcc tcaaaacgaa taagattcca
    18901 ctgatggtca tttgttgtac tatgtacata ataacattca attcccatag catcacataa
    18961 agctttcaag ctcctacaca ttccagaaca agcacctttt ttagtaacca atgtataagc
    19021 agtattggct ccctctgctg cgttccagtt aacatatgtt gtgttgtttt taatgtgatt
    19081 ataagcaacc ctaatcttag catctgtggt catgttcttt gtaattttct tgttcataaa
    19141 gtctgctgtt gcttgtgcta ccgaattaag ttgagcttgt gataactttg gaccatatat
    19201 actgttggtt ccagctacat aacttttcgg tgtctttgca gtaacatctt tagctgtcat
    19261 accataaact gttacactca tacatgaggt taaaaccatc attgcaatca atagtttagt
    19321 agctaatttc ctgatttttt tcatattagc attctccttt ataatatatt ttactttcaa
    19381 ttccctacca ttaattaagt tcattttaga tttaaaatat ggattatcaa catgtatgca
    19441 tagcaattca cgtggtcata gaatatttag atttcttgga aaatctgttc tatatttcct
    19501 atatagttcg tatacaatcc aactcacaaa cctgcatttt tgatatcatc acaataaaat
    19561 gataataatt aatatattca aaaatgttgg tctgtaacag gtatgtcaat tttccaatta
    19621 ttggtaatgg cgagagtgag gagaattgtc aataatacat ttaaaattaa aattgtatac
    19681 acttcataca ttctccccta acaacattat acagattata atttgtatat tcaatacatt
    19741 ttttggaaag tatagttata tcaaaatatg tgattatgta tagatacaat ccactatgga
    19801 gaaatataaa agaaaagagt ataacaaaca ataaaaaata agatgattca aattttattt
    19861 tgaaacatct tatttactct acaaattaag attatttggt gaattattta attattattt
    19921 tttattaaag aaattttaaa tcttaatata aaatacgcta ttatacataa taatccaaat
    19981 gcaaatgcta ctggaattaa catctccgat tcaaaataaa agaaatattc taagaaaata
    20041 tctggattta ctatattccc attcttgtct aaaaaatctt ttccattata tcctaaatgc
    20101 tgtataaaat tacttatgtt attataatct gcttccctct catataccat attagcattc
    20161 aatccagaag aacttaaatt agtgtttcca tctttcatat ctattacata tagtctaaga
    20221 gctttgactt tactatactt atctttatca agtatttttt caagttctaa cttatattca
    20281 ccaatctttg tcatatcatg tctttctgcc aattgcttta tttctccatc aagttctttt
    20341 cctaaattat acatattatg ttgtaaacta ttattgtaat accacataaa tccagaaatt
    20401 aaaaatgcaa ttatacttat gtaacctaat acttttttaa accccattac aattgattta
    20461 tgcctatcca atgataaaga tagttctttt attatattac ataattcata ctgcatttca
    20521 tcaccatcat caaatctttc aattgctttt ttacatgctt cttcttcatc taatccttct
    20581 aatttaaatt catttgcaga ttctataaga tggcatctca tttcctgttt taattctata
    20641 gtacttttat tgtcacattt tttatatagt ttatctaaat attcatctat tagtttcata
    20701 aattaatcca cctctcccaa aaattgattc ataacctttt taataaatct ccattcttgt
    20761 ttctttattt ctaaaaaatc tttaccttca ttagttaaat tataatattt tcttcttcca
    20821 cctgaacttt gttcattgct ataataagat ttaattaaat ttttagattc cattctttta
    20881 agtgcaagat acattgttcc ttcttttaat tcaaactcac ttctttctct tacttgcttt
    20941 gcaatttcat atccataaca atctttcttt tctaatatag aaacaattaa tatatcgata
    21001 taacctttta acacttcttt atttaactgc attatgtacc accgcctttt cacctactac
    21061 tctataatgt atagtactta tatttactat aataatacat agtactctat attgcaaggt
    21121 ttatattcta caatagactt aaaaataata aatagctgta tattaatatt taatctataa
    21181 atactaatat acagctattt atcaaatttt aaaaataaat ttttgtttta ttaatgttgt
    21241 cataacaaca tttacttggt tgaaat
//
LOCUS       DAELNY010000053        21235 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.053, whole genome shotgun sequence.
ACCESSION   DAELNY010000053 DAELNY010000000
VERSION     DAELNY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.053"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..133
                     /locus_tag="KPE55_001683"
     CDS             <1..133
                     /locus_tag="KPE55_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0492769.1"
                     /translation="YFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            1181..1942
                     /locus_tag="KPE55_001684"
     CDS             1181..1942
                     /locus_tag="KPE55_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0492770.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2007..2789
                     /locus_tag="KPE55_001685"
     CDS             2007..2789
                     /locus_tag="KPE55_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0492771.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            3056..3475
                     /locus_tag="KPE55_001686"
     CDS             3056..3475
                     /locus_tag="KPE55_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG0492772.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(3578..3844)
                     /locus_tag="KPE55_001687"
     CDS             complement(3578..3844)
                     /locus_tag="KPE55_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG0492773.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            4071..4772
                     /locus_tag="KPE55_001688"
     CDS             4071..4772
                     /locus_tag="KPE55_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492774.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4854..5462
                     /locus_tag="KPE55_001689"
     CDS             4854..5462
                     /locus_tag="KPE55_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0492775.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5786..6250
                     /locus_tag="KPE55_001690"
     CDS             5786..6250
                     /locus_tag="KPE55_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBG0492776.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            6887..7903
                     /gene="gap"
                     /locus_tag="KPE55_001691"
     CDS             6887..7903
                     /gene="gap"
                     /locus_tag="KPE55_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0492777.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            8298..9467
                     /locus_tag="KPE55_001692"
     CDS             8298..9467
                     /locus_tag="KPE55_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492778.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            9847..14100
                     /locus_tag="KPE55_001693"
     CDS             9847..14100
                     /locus_tag="KPE55_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0492779.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            14249..14743
                     /locus_tag="KPE55_001694"
     CDS             14249..14743
                     /locus_tag="KPE55_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0492780.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            14859..15236
                     /locus_tag="KPE55_001695"
     CDS             14859..15236
                     /locus_tag="KPE55_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG0492781.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            15442..15882
                     /locus_tag="KPE55_001696"
     CDS             15442..15882
                     /locus_tag="KPE55_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG0492782.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            16541..17848
                     /locus_tag="KPE55_001697"
     CDS             16541..17848
                     /locus_tag="KPE55_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492783.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    17934..18196
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18322..19125
                     /locus_tag="KPE55_001698"
     CDS             18322..19125
                     /locus_tag="KPE55_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0492784.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            19163..19774
                     /locus_tag="KPE55_001699"
     CDS             19163..19774
                     /locus_tag="KPE55_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0492785.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            19806..20477
                     /locus_tag="KPE55_001700"
     CDS             19806..20477
                     /locus_tag="KPE55_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0492786.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            20775..21137
                     /locus_tag="KPE55_001701"
     CDS             20775..21137
                     /locus_tag="KPE55_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG0492787.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         8651 a         2018 c         3700 g         6866 t
ORIGIN      
        1 ctatttttca aaagaaagtt tcttattaga agttgaaaat attggattta agtctgttga
       61 gttttttagt aatgttaagg gtgaagcata ttctgacgat agtatgacta tgggattagt
      121 tttacaaaaa taataatttt tttaaaatga ataaataaaa acttgaaacc tttaacaata
      181 aaataacata ataaaagctt acatattgaa aaatggaaac ttttatgtta tttttagtac
      241 attacaaatt tgcttatata aaaatcaata cttgaattat tatttttatg tgaaatttat
      301 ctatagtata tttttataaa caagtaatgt tattaaaatt aagtatgtag ttaaaagtgt
      361 atagcaaaaa ggctgttata ttaatgttag aggttcaggg aaaatatata aagcaaagta
      421 tttacagata atgtagattg cacagctata agttagataa tagaattatg taatcaagaa
      481 tttacaacaa agtataagat aaggataatg ctaaattgcc atacaggtgc tagatgtgta
      541 aatggattta attaatacta ttgataaagt gcaacaagca cataaaccaa ttgatgagat
      601 tattaaaact ataagagata tagttgacat aattaaaata ttggaacttg tttataattt
      661 taaatcaaat caataatgat taagattaaa ataatattaa ggcatcaatg ttataagagt
      721 acaatataag gagtactata tagtactcct tacttataca tattaggcaa taggaaattt
      781 tttcatagtt aaattttcaa gtatttttta ggaaacttta taattgagaa ttaagggtat
      841 agtatgtgat gaacttactg aatttataat taggttatat aatggaataa tcatcaatag
      901 ttttacttta gggaaaatat atgcttaagg aagtatagct tactaatatt attaaaatgt
      961 atatttaaaa tgataaaaaa agaattagac aagtatttag taaattgact atgcaaaatt
     1021 ctatcaagca ggaaccctat gactttagtc atgggaggtt cagtaggtaa cttgttgaat
     1081 taagtttata ttatttaaat aaactactta atttagataa tataaaaagt cagaatttta
     1141 agaatagagc tatataatct tagctattag gagaattact atgataggaa tatattttag
     1201 tggaacagga aattcaagat actgtgtaga aaaattttta caagaatatg atataacagc
     1261 aaattcttat tctattgaag ataatgaact tttacagcac atcaataatc atgagaatat
     1321 tatttttagt tatccggtgc aatatagtaa tgtgccaaaa atattgaaag attttataat
     1381 aaatcattca actttgtgga atggtaaacg tgtttttatt attgcaacta tggctctatt
     1441 tagtggagat ggctctggtg tgttatctag aatattaaaa aaatacggtg ctataactgt
     1501 tggtggatta catgtgaaga tgccagatag tataggagat gaaaaggtat taaaacattc
     1561 tttagagtat aatagaaagc ttgtaataaa agcagaaaag aaagtaacta aagctgctaa
     1621 aaaactgaaa catgggtcac caccaaaaga agggttaggt tttttgagtc atattgtagg
     1681 gttatttgga caacgattgt attttatcaa caaaacaaat atgtatacag ataaactaaa
     1741 aattaatact gaggaatgta ttgtatgtgg taaatgtgtt aatttatgtc caatgaagaa
     1801 tctagtaatt aaaaattgta tgattgttgc aaatgggcaa tgtacaatgt gttatcgttg
     1861 tgtaaataat tgtgctaaaa aagcaattac attgttaggt aaaaaagttg ttgaacagag
     1921 taatattaaa aaatatatgt aagaaattta aagttttatt gaatgtaaat tgatgtatat
     1981 tttatgttag aaagagaggt taaataatgg aatactacat agttgattca tttgcaacta
     2041 aactatttaa aggaaatcct gctggagttt gtgttcttga cagaagaata cctttagagt
     2101 taatgcagaa aatagcagaa gaaaacaatt taccagaaac agcatttgta gttaaaggta
     2161 aaggaaatta tgaattacga tggtttactc ctaaagcaga aattgattta tgtggtcatg
     2221 caactttagc agcagcttat gtaatatcaa attttattga tgttaatgta aaaaggatag
     2281 atttctttac tcaaagtgga aagcttgaag ttacacgaaa tggaaatcta tatgaaatga
     2341 tatttccaga aataatgcct attgaaatag agttatcacc acaacaagct aatttgatag
     2401 gctgtgtacc aagtgctgtt tattcttcaa gagatttaat cttattgcta aacagtgaac
     2461 aagaagttat aaattataaa cctaattacg cacaattgcg taaattgaca gactggttag
     2521 gtataataat cacagcacaa ggaagtaata cagattttgt atcaagatat ttttgtccag
     2581 aattagattc agaagaccct gttactggtt catcacattg taatcttatt ccatactggt
     2641 ctgaaaagtt gggaaaacac aaaatggtag ctgctcaact ttctaatcgt ggtggtataa
     2701 ttcagtgtga agtgttgaag gataatactg tgaaaatttc tggtgaagca gtcttgttta
     2761 tgcagggaac aataaagatt gatatttaaa taagtataat aactaattta ttatgaaaaa
     2821 taattaattt tcttagtgtt aacaataata aagtagagtt aaaaaacttg atttcactta
     2881 aatcaatatg atagatgttg atactaagat tgacaagcat gattaaaaaa gataatattt
     2941 aatctataaa gataaaaaca cagtctggtt ggtagtccag acgcagcaat agctgtcagt
     3001 aacctgcctc cttggttgtc cgttctttag aaagtaattt aaggaggaac tagcaatgga
     3061 caaaattagt ggaaaattaa cagtcttatt tgaagaacca ttttgggtag gtatatttga
     3121 aagacaagat ggtaaaaaat atgaagcatg tagggtagtt tttggagcag aacctaaaga
     3181 agtagaagtg tatgaattta tacttgaaag atttttctca ttagattttg gtagcattaa
     3241 attagaaaaa aatgtgacca aagacaacat tggatacaaa agaatgcaga gaaaagtaaa
     3301 aaaggaacaa gaaaaagaaa ctatagggac taaggctcaa aatgctctta agcttcaata
     3361 tgaagaaaga aaacaggata gaaaaaacct agctaaaaat agaaaagaag aagaaaaaga
     3421 aagactattt aatttgaaac aagaaaagag aaaagctaaa cataagggtc attaatttta
     3481 attaaatatc tttttaataa ttgctagaaa aaggtgtata aaatttttaa ttttatacac
     3541 cttaatttat tgaaaagaat aaggttttat atttcaatta ctttaaaata taaaaatata
     3601 ctaataaaaa ttgtaaaaat agtataaatg gtataccaaa tgtaaattta atgtgtttag
     3661 ttttatgtct aaaagaatac attccaatta tagaacctat actacctcca attactgcta
     3721 tagacataag agtagcttct tttattctcc attcgtttct aattgctctt ttcttatcta
     3781 tgtacattga aaagaaagca atagaattaa tcactataaa atatattaga ataaaatttt
     3841 tcacagtctt gcctccataa aaatatatta tacattataa aacaagatat ttgttttgga
     3901 aagttaaatt tgtaattagt acatataaaa tatttgataa gaacaaatat aatattaatt
     3961 ggaaaattta aaaaaattaa acaattagca aatagggggt atatttataa gacacaaagt
     4021 gataaaatct atttagaaaa taatgtaaaa taacaggagg ataaagcaaa atgaaattta
     4081 tagtagaaaa agaagttttt gataaattag aaaatgtatg ctttggtgta gtagttgcaa
     4141 aaggaataga taatactaaa gaaatagaaa gaataagcaa ccttttagat ataagtatag
     4201 atagagtaga agattacttc aaagataaaa aagtaaagga atcagaagaa attataccat
     4261 atagagaggc atttagaagt ctaggcatga acccaaataa atttatgagt tcgatagaag
     4321 caatgacaac aagggttgct aagaataaga aacttcctca tataaatcct attgtagatt
     4381 taggaaactc tatttcttta aaatatttac ttccaatggg tgctcatgat atggatttta
     4441 gaaatgatga tgtatatgtt agattttcta aaaaagggga taaatttgta ccatttggag
     4501 aaactgatgt tgaattaatg gaagaaggag aattaattta ttcagttgga gacatggtaa
     4561 aaactagaag atggatatgg agacaaggag aagaaggaaa aattactaat agctcaaaaa
     4621 atatattttt tcctatagat ggttttactg atgctaattt agataaggta atgagtgcaa
     4681 gagaagaatt agcaaaattg ttaaaggaaa tatttaattg tgaaattaaa gtaggttttg
     4741 tagataaaga taatcctgaa atggaaatat aataagttgt tgaatacttg ttaagatatt
     4801 atatttactt aatgatgtta aaaaggtatt aaaaaagaaa ggtcgtagta aatttgaaaa
     4861 aaactataat aaagaatgta aatccaatga ttgaaaataa gtttatagga ctttttgaaa
     4921 tagagtataa aaacaaatta gatgaagata aggtttggat ggtagcatca agaaaaagta
     4981 gtgagcaatt aaaaaatatt tatttgaaga atgaagaaga tagtgtagat gcagttgtaa
     5041 tagttgggct tcataaaagt agtaaaaaat taattttaat aagacaattt agagtaccta
     5101 ttaatggata tatatacgag ttaccagcag gtcttgttga tgaaggagag tctatagata
     5161 tttccgttga aagagaactt agagaagaaa caggacttac tcttttagaa attaaaaaaa
     5221 ataaaagtat tgataaagtt tatttatctc ctggaatgag tgatgaatct gttgcctttg
     5281 tttattgtat atgtgatggt gatataacag atgaattttt agaaccagat gaagagattg
     5341 aagcgctttt aatttcgcaa gaagatgcaa aagagatttt acaaagtaat cataagatag
     5401 acacaaaagc atttttgatt ttacaaatgt ttgtgagttt aggagctaaa ttatttgaat
     5461 agatatatag aaaagaggtt atatagaaga gtaaaataat aaagtttatt ttacttgtgt
     5521 aaattataat ctcttttttt atacaataaa gtataatttt gtgtattttt aaataagtga
     5581 tttgactctt aaattttcat aatatgttat aaaccaattt aagatactac aataattaaa
     5641 attccaaaat tatttaacat atttgcaatt aaaatgagaa aatgtaaagg ctgaatgatt
     5701 gaaaaatttg atgtaaaata gtagcttagt ttaatactat agtttgtagg gttaaaaatt
     5761 agaaattgta agggagataa attctatgaa aaaattagtt attattttaa ttagtatcat
     5821 tagtatggtt agcttgacag cttgtaaaaa aagcaatgaa agtaaatata ataatttaaa
     5881 caacaaagaa tccggacaaa caaccattga aatggactta gataaaaatt atgatacttc
     5941 agacccattt gttaatgcac gtttgttttg tgtgtctaat gatatagatg ttttagacac
     6001 agagatttct tttaaaatgg atggagatag tggtattgtg gaaataaagg acaataaaac
     6061 agatgaaact ttatggagta atacatggca tggaagcgtt gataatgata cattcacaat
     6121 ttctctttct aatatacaaa aagagaaaga gtatgctata tgctttactg gaattaaaat
     6181 aaatcatgca gttgtaaagg ttacttttga aagcaattta gttaaggaga aagaaaaacc
     6241 ctcaaaataa attccagctt ttctataact tgatattcag tacataaaag cagttgatat
     6301 ttatgattga ctgctttttc ttatcaaaaa ggataggttg tgcattttta cttgaaatag
     6361 tttatgcaca tcaaaagttg gtgagcatga tattggaaat ggtaagcatg aaatcttgat
     6421 aatacaagga aataaattta agttttcaat taagtatatt tccataattt agttaacaat
     6481 aagctaatta ttatttatat tttgatatat aaataataat tgtagcaaat tttaaaaaat
     6541 catatatttt ttgaactaaa taaaaattta tttttattat tttaaattag aggagattct
     6601 tgaagatata ttatttttaa aaataagtat agtttaaaaa ttcaaaaaaa agatggcaaa
     6661 atatgtccca agggacattg agtaaaataa atgatttcag cttatttaca ctaaaaagta
     6721 catactaaat gaggggtaaa ataaagaaaa atgatatact aattgtcttt tgataataaa
     6781 aaaacatgga atttgattat ttaatcaaga aaaagtattg aacaaatgat ttatttagtg
     6841 tacaataatc atgtaaaaac atagtaatgg agggagacat ttcataatga aaattaaggt
     6901 aggaatgaac ggatttggaa gaataggaag agctgtatta agaatagctc aagaagaatt
     6961 aggagataat atagaaattg tagccataaa tgcaagagca acaacagaat ctttggcaca
     7021 tttatttaca tatgattcat gctatggaac ttttagaggt gaagtagagg caaaggatga
     7081 agacactcta gtagtaaact ctaaagagat aaaaatatta agatataatg acccagaaga
     7141 attaccttgg aaagaattgg gggttgatat agtaatagaa tcaacaggat tatttacaca
     7201 aagagaaaag gcagaaaaac atattaaagc aggagcaaaa aaagtaatta taacagctcc
     7261 aggaaaaaat gaagatataa ctatagttat cggagttaat gagaaagaat atgataatga
     7321 aaagcacaat ataatttcaa atgcatcttg tactacaaat tgtctagctc cttttgctaa
     7381 agtattggac gaaaaatttg gtatagtaaa agggttaatg actacagtac attcatatac
     7441 taacgatcaa agaatattag ataaaagcca taaagatttg agaagagcaa gagcagcggc
     7501 tgaatctata atacctacca ctacaggagc agcaaaagct gtgtcaagag tactaccaca
     7561 attagaagga aaattaaatg gtttttcact tagagtacca acaccaacag tttcacttgt
     7621 tgatttagtg tgcgaactta aagaaaacgt tacatctgag caagttaact ctgtgttaaa
     7681 agatgctgct gaaggagaat taaaaggtgt tttaggatat tgtgacaagc cacttgtttc
     7741 tatagattat aaaggagatt caagatcatc tattatagac gctctatcca caatggttat
     7801 agaagacaat atggttaagg tagtttcttg gtatgataat gaatggggat attcttcaag
     7861 aactgttgac ttagtaaaat atgtagctga aaaattaaaa taatatatat ttcttaaagc
     7921 aaattttaaa aaaatttttt aaaaaaatta tataaaacta aaaaaaaaga ttatgaaaaa
     7981 tattgaagga aaaatttttt cataatcttt tttctttcat gcaaaaagat acaagttaca
     8041 acttatattt gcatattagg tcgttagaga agtatatata tatttagtaa ggaatcatac
     8101 tttttataaa atgaaagata agatgtcgaa aacttgcata aatctgaaaa atttttatta
     8161 ttaaaatact gatttccatt ttaaaaatga taaaaaaatg acaaataaag ttgcaaatag
     8221 taatacttat aagtataatt aaaaaagaga aaaggttttg ttaaacttta taacaaaaaa
     8281 agaagggatg attcaaaatg gagaagttaa cagacaggag acaggttaag gcagtaagca
     8341 tggaaacctt cattttccta atattactag tagttggttt tgggtatgtt ggtagtatta
     8401 tgggagcagg aatgatgttc aaagtaataa tgagtacagc acatgcttta ctattggaga
     8461 cagttttctt aattatggct atggcagttc ttgcaggcgc attaagcgca ctactttcag
     8521 agtttggagt aatagcgtta attaataaaa ttttcgctgt atttatgaag cctttatatg
     8581 gactacctgg tgctagtata gcaggagcta taacaactta tttatcagat aatccagcta
     8641 ttataccttt tgctaaagat aaaacattca cacagtattt taaacaatac caagttccag
     8701 cattatgtaa cttaggaaca gcctttggta tgggacttat cttaacaaca tttatgattt
     8761 ctcaaggtac agaatatgta cttccagcat taattggtaa tttaggtgca attataggta
     8821 gtgtcataag tgttagaata atgttgacat ttactaagaa gttttataaa tacaatccgg
     8881 aagaagataa agcaacagga acattagaga aaaaggatga atttagagaa atcagagaag
     8941 gaaatgtatt tcaaagagct ttagatgcaa ttcttgaagg tggtaaaatg ggggtagata
     9001 tgggaatggc tataattcct ggagtcctag tagtatgtac attggtgatg ttattaactt
     9061 ttggaccatc tacagatcct gttacaggtc aagaagttta tacaggagct gcatatgaag
     9121 gaataaaatt acttccagtt attggagata aacttggatt tatattagaa ccattatttg
     9181 gatttacatc accagaggca atagcattcc ctataacttc attgggagct gttggcgcag
     9241 caatgtctct agttccagag tttataaagt caggagctat aactcctaat gatatagcag
     9301 tatttacagc tatgggtatg tgttggagtg gatatttaag tacacatatt ggtatgatgg
     9361 atgcattaaa tgctagacag ttggcaggta aagctattct ttctcataca ataggtggtt
     9421 tatgtgcagg agcagctgct cactttatat ttacactcgt aggataaatg taatcagcta
     9481 agataaaaaa taatatacaa aaaatggttg ttgatatgta ataagaattt acatatcaac
     9541 aaccattttt ttctgactaa agataattta taatacataa acattaagtg gatattagag
     9601 ttaagttata tttatgtaaa aagtaacaaa aatctgtaga aatttatgac aaaatttagt
     9661 acactaacta tatgttctta tttaaaattt aaggttagaa ttaggaatta cctagtagag
     9721 taagctttga ttaataaaag aatcataaaa taaaaatatt agtcataaag attataaata
     9781 acttacttaa aataaagcca tagaacttac aaaaattaaa aaagcatata aatatggagg
     9841 ggtacaatgg atgaattatt tcatatagga attgatgtag gttctacaac ggtgaaggtt
     9901 gtggtactta ataatagtaa taatatagtt cataaagaat atagaagaca ttattctgat
     9961 gtaaaaaaat cagttaaaga agtactaaat ggtatatatg aaaaattggg agatataaat
    10021 actaccatta taataactgg ttcaggtgga attggaatat caaaaaaatt aggtgtaaaa
    10081 tttgttcagg aagttatatc tagtacaaaa tcaatagagt attttcatcc tgaaactgat
    10141 gttgtcatag aacttggggg tgaagatgca aagataacat atctaagtgg aggaatagac
    10201 caaagaatga atggaatatg tgcaggtggc actggagcat tcatagacca gatggcatca
    10261 cttttaaaga cagatgccag tggattaaat gaacttgcta aagggtataa tgttatatat
    10321 cccatagctt caagatgtgg tgtttttgct aaaacagata ttcaacctct tataaatgat
    10381 ggagccaatc agactgatat agcaatgagt attttcaatg cagttgtcgt acaaactgtg
    10441 agtgtccttt cttgtggaag aaaaatagaa ggaaatgttg cgtttttggg tggtccattg
    10501 tactttttat ctgaacttag agaagcattt aaaagagtat tagatttaaa agatgaaaac
    10561 ataatcttcc cagaaaatgc acaactttat attgctattg gagcaggatt attatcagta
    10621 gatgaagata atatacaact aaaatctcta atagaaaaat tggatagtat aaagaatatt
    10681 gaagatggag aagtaaattt attagaacct ttatttaaag ataaaaatga gtatgaagaa
    10741 ttttcaagaa gacatgaaaa agaaaaaata aattatgtgg acataaattc aataaaaagc
    10801 aattgttatt taggaattga tgcaggctca actacaacta aagcagcact tattgatgag
    10861 gatggaagac ttgtatattc ttattataat agcaatgaag gaaatccatt aaaaacaacc
    10921 attaaagtta taaatgaagt atatgatata ttaccaaaaa atattaaaat attgagttca
    10981 actgtaactg gatatggaga aggtcttatt aaaaaagctc ttaaaatcga taatggagag
    11041 atagagacta tagcacatta caaagctgct aaatttttca acaaagatgt agattttata
    11101 cttgatattg gtggtcaaga tatgaagtgc ctaaaaataa aagatggagt aattgataat
    11161 ataatattaa atgaggcttg ttcatcaggt tgtggctcat ttttagagac atttgcaagt
    11221 tctttatcta tgacaataga agaatttgcg catgaaggta tttattctca aaatcctgta
    11281 gatttaggtt caagatgtac tgtatttatg aattctagag taaaacaagc tcaaaaagaa
    11341 ggtgcaagtg ttggagatat atcagcaggg ctatcttatt cagtaataaa aaatgcttta
    11401 tttaaagtaa taaagattag agatttaaat gagattggta ataatattgt tgtacaaggt
    11461 ggaacctttt ataatgattt ggtgcttaga agctttgaaa aactaattgg aaaaaatgta
    11521 attagaccta atatttcagg aattatggga gcttttggtt cagcacttat agctaaagaa
    11581 aaatatgaaa gtggatacaa aacatccttg ttatctaagg aagagttgaa taatatcaag
    11641 ctagaagcaa gtgtaactag atgtaaaggt tgttcaaatc attgtcttct tacaataaat
    11701 aaattttctg ataatgaaat ttttatttca ggtaatagat gtgagaaagg tgaagctatt
    11761 tatggtgata aaaaaatagg attagaaaag aaaaaaccaa taaatttatt taagtacaaa
    11821 tacaatagaa tttttagata caaaccattg gaaaaagaag aagcaaaaaa tggggaaata
    11881 gggataccaa gagtattaaa tatgtatgaa gattatccat tttggtttac ttttttcaat
    11941 gctatgggct ttagagttgt actttcagat aggtcatcaa aacagttata tgaaactggt
    12001 ataactagta ttgcatctga aacagtttgt tatccaggaa aattagttca tggacatata
    12061 gaaaatttaa ttgcaaaagg tataaaaaat atattttatc caagtgtaac aaatgaaaat
    12121 aaagaggatt caaatgcaga taattattat aattgtcctg ttgtaatatc atattctgaa
    12181 gtaataaaaa acaatgtaga gaatataaga aataagaaca taaattatat aaatccattt
    12241 ataagtctaa atgacaagga aaaattgaag aaaagactct atgatgaatt atcaagacat
    12301 ttttctgata taaaaataac taaagaagaa gttaatcatg cagttgataa agctactgat
    12361 gaacagaatt tatttaaaat ggagatacaa gcagcaggtg aaagagcact taaagaaata
    12421 aaagaaagaa atatgaaggg gattgtgtta tgtggaagac catatcatat agacccagaa
    12481 ataaatcatg gaatgccaga gcttataaat tcattagata tggcagtgct tactgaagac
    12541 tctatatgtc atttagccaa tgtacaaaga ccacttagag ttgtagacca atgggtatat
    12601 cattcaagat tgtataaagc agctagtttt gtaagagata aaaactattt ggaacttgtt
    12661 caacttaatt catttggctg tggattagat gcagtaacta cagaccaggt tcaagaaata
    12721 cttaatgaaa aatcgaagat atatactatt ataaagattg acgaaggcaa caatttagga
    12781 gcagctaaga taaggatgag gtccttaaaa gcagctatgt ttgagagaga aagcaaaaat
    12841 atagatgtaa aaaatataaa ggtaaacagg atacagtatg ctaaaaataa tcagataacg
    12901 aacaaacata ctatattagc accacaaatg tctccaatac atttccaatt ccttgaaaaa
    12961 gctgttaact taagtggtta taatatagaa attctaaaag atactgatag tagtgtaatt
    13021 gaagaagggt taagatatgt aaataatgat gcatgttatc cagctataat agttgtaggt
    13081 cagctaataa cagcattaaa atctggcaaa tatgatttaa ataacacttc tgttactata
    13141 actcagactg gtggaggttg tagagctaca aattatattg gattcttaag aaaggctata
    13201 tacgatgctg gatttaaaga tattcctgtc attgcactta gtgtaaatgg aatagaagat
    13261 agtggtataa tggataatat atcactaaaa ttaatcaata gactctttat gtctgttgtt
    13321 tatggtgatt tgctgatgaa agtactatat agagtaagac catatgaaaa agttactgga
    13381 agtgcaaatg ctctatatga aaaatgggtt gatatatgca aatcttctct tgttaaagca
    13441 aaaatttctg tatttactag aaatataaaa gatatagtaa aagaatttga taatttagag
    13501 atactagata tcaaaaaacc aaaagtaggt ttggttggag aaatattggt gaaattccat
    13561 ccaattgcaa ataataattt agtagatata ttagagcgag aaggtgctga agcagtcgtg
    13621 ccagacctta caaatttctt cttaagttgt gcctttaata ctatatacaa acacactcat
    13681 ttagaaggaa gtagaaagag tagaatgata ggagaagcat ttatatacat aactggaata
    13741 tatcaaagag tatataaaaa agcacttgat aagagtgaga gattttatgc tccagccaat
    13801 ataaaaaatg ttgctaaaag tacagaacca gtagtgtcat taggtaatca aactggagaa
    13861 ggttggcttt taacaggtga gatggttgaa ttattaaatg aaggtgtaga aaacattatt
    13921 tgtatgcaac cttttggatg tttacctaat cacataattg gtaagggttc tataaaagaa
    13981 ttaaagaggt tgtataaaaa tgcaaatata atacctattg attatgaccc ttctgcaagt
    14041 gaggtaaatc aacttaacag gataaaactt atgctttcta aggcttttaa aaatatataa
    14101 gatttatatt ttgatatgtt aaaagttaat gaatttaaaa atatactcta tattaagtat
    14161 ctataaaaaa gagttatcct attttttagg gtaactcttt attagttaaa aaattatatg
    14221 tatactgata ttaaaggggg gaagtaatat gagtttagta tatgatgttt ataaatcaaa
    14281 attaggaaat ttgtacatat taagtgaaga tggttttata gttagtatat acataggaga
    14341 tgaaaaattt aataatttaa aaaatgaatg tattaaaaaa ggaattgata taaagagaag
    14401 taaggaagat ttaaaggaag caataagcca gatagatgat tactttaatg gaaaaagaaa
    14461 agtctttaat ttaaatattc gtattgaagg gactgaattt caaaaatcag tttataggga
    14521 aatgttaaaa ataccttatg gagaaacatt gtgttattca gatatagcaa gaaatataaa
    14581 caatccaaaa tcagtaagag caataggaca agcaagtaaa agtaataaaa ttccaataat
    14641 aataccttgt catagagtag ttggcaaaaa agatattggt ggatatatgg ggaatcattc
    14701 tgatttgaaa gagatactat taaatttaga aagacagagt taacttagaa agaaatgttt
    14761 ttattgttaa tatttgatat aataatagta aaaagaagta ttttttacaa aaacaatgaa
    14821 gaatttattt taatttataa aaatgagggg gaattttaat gtgtgataaa gttatagcat
    14881 caaaatatca tgagaaggga tttaattgtg cagaatcagt tataaaagca tataatgagg
    14941 aatttaatac agatatacca gtttgtttag gaagtggact gggttcaggg tgtggggttg
    15001 ctagtttatg tggtgcagta aatgcatcaa atataataat tggatatgta aaaggcagaa
    15061 accacgaaga tgaaagtact aaagctaaag tatatgctaa agatttaact acaacagtaa
    15121 gaaaagagta tggttcagag ttatgtatag acctaaaaaa agatttagtt gcttgtagag
    15181 aaataatgga tttcgcatat gatagtctaa aggaaacttt aaaaaaagaa ttataagaaa
    15241 ataagctatc aagtataaat tatttgatag cttattttta tagtcgatac taaataaatc
    15301 aaatatattt tagaattatt atttgtaaat aaacagcaaa taattttagg atttttattt
    15361 aatatagttt ttatttatat ttatttttga atatgttata ctttgtttaa ataaatattt
    15421 gtaatgaggg agatgagaag attgaagaat ctcaatttac ttattaataa attatattca
    15481 aaaaatcata atgaagcata taaaactttt ttattcttag aaaatgaaag tttaaaatca
    15541 aatattacat attgtttttt tgactcattc ctagaaatga ttgataacga aaactcatat
    15601 attcgaacaa ggggattgtt attaatctct gcaaatgctc agtgggatat tgataataaa
    15661 attgaaataa atatagatag tatactaagt catatcgtag ataaaaaacc ttctgtatca
    15721 agaatgttta ttaaatccat acctaatatt acaaagtata aagaaaatct tattaatcgc
    15781 ataaaaatgg aattaagtaa tgcagatata agtatttata ataataatat gaaaccatta
    15841 gtagaaaagg atataaacga tacattatct aatattagtt aaatattaat atagaaattg
    15901 aatattatct agttacaatt atttataact aagataataa aacaacaatg gtgctaattt
    15961 atatgtataa attaattatg aaaaatttat ataagtaaaa tatattaaca taaaggtaaa
    16021 attttgtata ctaaaaggga atatttacag tttaatggca gttttaaaat aataaattaa
    16081 agctgtcatt tttatttaaa aatttttaga aaaagtacaa tagtgttaat gtgtgaatta
    16141 gataaggtgt atataatagt ttagatgata tgatataaaa tatgttgtaa attaaatgtt
    16201 ttctttgaca atatatacac aaggatgata atatctatat gaataaaaga tagtgtgtaa
    16261 tttgataata tgtaattata acattatgat ataattacaa taaagtatgt aataatctgt
    16321 agaaatttca agctagttat ctaagttttc ggataactaa atctaaacgg aatacataat
    16381 gtattaaata aatgatgata ctaaaatgat actattttta tagaaattta ttgaggtata
    16441 taattaatga aaataatata ttaaattata taaattaaat tatataaatt aaattatata
    16501 atatattgag ttttacataa aagcaaaggt gggaataaaa atgaaagaaa ttgctgatgc
    16561 tataacatca gagtatttaa gctatggtat caatgaagat attagtatct atgaaggtag
    16621 attttgcata tatttagata aaaaatatag atgtaatggg aaaatttatt ataaaatgac
    16681 acctccaata tctataagtt ttaaagcaga cataggttgt gttgaagaga tagacaatga
    16741 agatgacaat ttagctttag attatgataa tgctattctt gaagttcatg gatataaaat
    16801 tatttctata acaataaata ctttaagtga atttagtgta gaaggttata tcaatgatga
    16861 ctgcataaaa tctaagaact cctatgttga atatgtagat tttaatataa taaacttaga
    16921 taaaatacct ggaaaactca ttaaatacaa tgataaggta tacgcaggta gaattgaatt
    16981 tgatataaat gattatgttg taactataga caagcgatat gattatagaa aagaattaaa
    17041 atcagaatta aagtctaaat ctggagcaat aataactcat ataggtagga ttagaagaaa
    17101 agatggtaga atttttagaa caaataatac aataaatctt ttagatagga tatcaacagc
    17161 tttgagtttt atgtgtggaa gatatgtagg gttttgtttg gccaaagggt atagaagtgg
    17221 taatgaagtt tatagaatat ggaatgaaaa tcaaatatct ccttttagat atgtaccaac
    17281 atggtcagat acactttcaa attattataa tatggaaaaa tatataagcc ttatgtgtaa
    17341 aaagctagaa gatttttatt atggttctgc tataaagagt gttgttgatt ggtacataga
    17401 atctcttgga agtgctacca tggaaaacaa tataatatct gtacaaattg cacttgaaac
    17461 tttgtcatat gtaattctag tggaacaaaa taagatactt acagatgaag tatttgactg
    17521 taatttggca tctaaaaata taagactctt acttgataca tgtaagatac catatggtaa
    17581 acatgaatta aatatatttg ataatataat aaaaaataaa tttgatgatg gagtggattt
    17641 agttatttat ttaagaaata gcatagttca tccaagtaga aagactcata gagctgtgtt
    17701 agaggttgaa gatatttgga atataatttc aataggaacg agatatattg aacttgtttt
    17761 actctttatt ttaggttata gaggagaata ttcaaacaga ttagtagaaa gatgttatgg
    17821 tgaagtggaa gttgtacctt ggaactagat ttttatattt attttaaaat atatattgac
    17881 atatattgcc ttatgctata aaatcaatat cataataata attcagaata acattaaatg
    17941 ctaagaaaag aaatagtaaa agtagttaat cttaaacaga gagtttccga ttggtgagag
    18001 gagcaataga ttttactttg aacacatctt tgagcaggac tctgaaagag tagaaatact
    18061 ttattaggag atttccggaa tccttgcacc cgttatagtg ctagagtata ataattttat
    18121 tgtacttaaa gaggttagtg tcgtgaggca ttaatgaaca gaggtggaac cacgagagtt
    18181 aactcccgtc ctctagcaga agctagaggc gggagttttt taatataatt ttttaagcgc
    18241 gcagagaatt atattgcaat agatacactt tttaaaaaat aatcaaaata ttttaaaaat
    18301 taaaaggcga gggagaatat tatgaaaaat atattaaaaa aagtaggaat atttacaatt
    18361 atgttaggtt tattaggtgg agttgtaggt tgttcaaaac cagataatga aaaagataag
    18421 gatgcttcaa aggaatctaa aaaagaagta gttgtagggt ttgataatac gtttgtacca
    18481 atgggatttt tagatgaaaa gggagataca gtaggatttg acgtagactt agctaaagaa
    18541 acatttaaaa gacttggaat ggaagttaag tttcagccta tagattggtc aatgaaagaa
    18601 actgaattaa atgattcaaa aactgtagat gtattatgga atggatattc tataacagac
    18661 gaaagaaaga aaatagtatc atatacagaa ccatatttac aaaataagca gataatagtt
    18721 actctaagtg actcaaaaat aaattcaaaa gctgatttaa aagataaaga agttggaaca
    18781 caacaaggtt caacagcact tgatgcagtg gaaaaggata aagattttat gaatagctta
    18841 aaaggtggag caccagttct ttatgatact tatgataaag cacttagaga tttagagata
    18901 ggtagaacta gtgcagttgt tggtgatgaa gttttaatta gatactatat gggacaaaag
    18961 ggtgaagaca aatacaaggt attaaaagac gattttggat tagaagatta tgttgtagca
    19021 acttctaaag aaaatccaga actttgtgag aaaataaatg aaacattaaa agaaatgaaa
    19081 aaagatggaa cttttgacaa gatttatgat aagtggttta aatagaagat aagttagctg
    19141 ttaagaaggg ggcactaaag gcatgttaca aggtttagag atagttatag caatgttttg
    19201 tataacattg atagtatcaa ttccattggg gattggagtt gcatttttaa gattatcaaa
    19261 gaataaatta gtaagtggta ttacacaatg ttatatattg ataatgagag gtacaccttt
    19321 acttttgcaa atgatagtca tattttacgg attacctctt ttaggaattg tatttgacag
    19381 atttacagct ggagtagtag ctttcttctt aaattatgca gcatattttg cagaaatatt
    19441 tagaggtgga atacagtcaa tcgatagagg acaatatgaa gcttcaaaag tactaggatt
    19501 tgataagttt actatgtata agagagtaat ctttccacaa gtttttaaaa gaatactagc
    19561 acctatttca aatgaagtaa tcacattagt aaaagataca tctttagtat atatattagg
    19621 attaaatgat attttaagaa tatctcaaat agcaatgaac agggaagcaa gtctgttacc
    19681 attatttgag gcaggagcga tatatcttat atttgtagct atacttacta agggatttga
    19741 attacttgaa aaaaaatatt cttattatag ataaagttac aatattatag gaggaggttt
    19801 tttatatgtt gaaaatcaaa aacttaaata aatcatttaa aaaaaataga gtacttaaag
    19861 atatttcttt tgagttagaa gaaggtcaga ttggagtttt acttggtaag tctggtgcag
    19921 gaaagaccac aatattaagg tgtataaatg gtcttgaaga atttgatagt ggagagatta
    19981 taatagataa tgaggtaata aaaaataaga gagatatggc aaaaatacga ggaaaaatag
    20041 gtatggtttt tcaaaatttc aacttatttc ctcatatgac agtccttgaa aatataattg
    20101 aatccccagt taatgttttt aaagttccta gaaaagaggc tgaagagagg gctagagaac
    20161 ttttaagatt agttgattta gaagataaat taaattctta tccatttgaa ttgtcaggag
    20221 gacagcaaca gagagttgct atagctaggt catgtgcatt gatgccaaaa gtcctttgtt
    20281 ttgatgaacc tacttctgca cttgatattg atactattca aagagtagtc aatataatga
    20341 acagacttaa agacaaaggt atgacaatat taataattac acatgatgtt gtcttctcaa
    20401 ataatgttgc agataaaatt ataagtataa aagatggtat agtagaaaat gttcaaataa
    20461 aagaaaaaat tgtttagagt tttataaata taaactaggg tagaatttta taatataaat
    20521 aaagggcaag taaaatcaaa tatactatgc ccttttgtta tctcaaaatt taatcttata
    20581 ttttatgttt aagttacatt ataatttata ataaaaataa aaggactatt taaatatata
    20641 atgtgaataa tgtttaataa tttgaaatat gataattttt tatgttgaaa gcttataatg
    20701 attatgatat aaattggtta tagtatacaa tttatgggta taacaataaa aaattagaaa
    20761 ggaattttta taaaatgatt aaattatatg gatatacaaa atgctctact gttaaaaaag
    20821 ctaaaaattg gcttaaggaa aataatttag aatttgaaga tatagatatg gttcaaaacc
    20881 ctcctagtaa agaggagtta aaatcaattt ataagactag tggatatgat ataaagaaat
    20941 tttttaatac aagtggtatg aaatatagag aattgggtct taaagatatt gtaaaaactg
    21001 aatctgatga taaactttta gaaatattgg taagtgatgg tatgcttatt aaaagaccat
    21061 tacttttgga tggtaaaaat gtattattgg gttttaaaga agatgtatgg aaaagtactt
    21121 tgctaaaaga agattaggaa gagataggtg tgttgattaa aattattaat taatacacct
    21181 ataaatttat gtattaaatc taatctttat aaatattaat tagcaaattc aaatc
//
LOCUS       DAELNY010000054        21231 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.054, whole genome shotgun sequence.
ACCESSION   DAELNY010000054 DAELNY010000000
VERSION     DAELNY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.054"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(47..1384)
                     /gene="xylA"
                     /locus_tag="KPE55_001702"
     CDS             complement(47..1384)
                     /gene="xylA"
                     /locus_tag="KPE55_001702"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG0492788.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFSGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(1480..3030)
                     /gene="xylB"
                     /locus_tag="KPE55_001703"
     CDS             complement(1480..3030)
                     /gene="xylB"
                     /locus_tag="KPE55_001703"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG0492789.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVILDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            3238..4398
                     /locus_tag="KPE55_001704"
     CDS             3238..4398
                     /locus_tag="KPE55_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0492790.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(4554..4958)
                     /locus_tag="KPE55_001705"
     CDS             complement(4554..4958)
                     /locus_tag="KPE55_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0492791.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(4979..5461)
                     /locus_tag="KPE55_001706"
     CDS             complement(4979..5461)
                     /locus_tag="KPE55_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0492792.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(5481..6290)
                     /locus_tag="KPE55_001707"
     CDS             complement(5481..6290)
                     /locus_tag="KPE55_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0492793.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(6283..7062)
                     /locus_tag="KPE55_001708"
     CDS             complement(6283..7062)
                     /locus_tag="KPE55_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0492794.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(7062..9140)
                     /locus_tag="KPE55_001709"
     CDS             complement(7062..9140)
                     /locus_tag="KPE55_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG0492795.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(9939..10460)
                     /locus_tag="KPE55_001710"
     CDS             complement(9939..10460)
                     /locus_tag="KPE55_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0492796.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            10626..11192
                     /locus_tag="KPE55_001711"
     CDS             10626..11192
                     /locus_tag="KPE55_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0492797.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(11409..13472)
                     /locus_tag="KPE55_001712"
     CDS             complement(11409..13472)
                     /locus_tag="KPE55_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG0492798.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(13853..17188)
                     /locus_tag="KPE55_001713"
     CDS             complement(13853..17188)
                     /locus_tag="KPE55_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0492799.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGQEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(17237..17665)
                     /locus_tag="KPE55_001714"
     CDS             complement(17237..17665)
                     /locus_tag="KPE55_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492800.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(17655..18035)
                     /locus_tag="KPE55_001715"
     CDS             complement(17655..18035)
                     /locus_tag="KPE55_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG0492801.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(18068..18376)
                     /locus_tag="KPE55_001716"
     CDS             complement(18068..18376)
                     /locus_tag="KPE55_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492802.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(18409..19593)
                     /locus_tag="KPE55_001717"
     CDS             complement(18409..19593)
                     /locus_tag="KPE55_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG0492803.1"
                     /translation="MVDEAKILDVVSISGENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(19597..21060)
                     /locus_tag="KPE55_001718"
     CDS             complement(19597..21060)
                     /locus_tag="KPE55_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG0492804.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFNDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIMGRVKALEKEIVDGLEELERMLEV"
BASE COUNT         6704 a         3864 c         2206 g         8457 t
ORIGIN      
        1 atatcctgtt attttattca aacatccaag tttaattact acacaattac ttattagaaa
       61 ttgtatctac ttcatatata tatctgttaa gtatagcttc tagcatttct tgtcttccag
      121 acttattctt tataactggc atttgcagtg cataagattc taactcttta aatccaacct
      181 taccttctac tatatcctta cctatacctt cactaaaact tgcatatctt tctttagtaa
      241 agttctcaaa tacctcatct tctaataatt tatgagctat taataatccc tttgcaaatg
      301 tatccattcc tgcaatataa gctaggaata agtcttctac ctcaaaagat gctcttctta
      361 ctttagagtc aaagttaaat cctcctggtg ctattccacc ttgctttaac acttcataca
      421 tagcaagtgt tgcatcatat atatttgttg gaaattggtc tgtatcccaa cctaataata
      481 ggtctccttg atttgcatct atactaccta aaacattgtt tattcttgct atatttaatt
      541 catgttggaa agtatgtcct gcaagtgttg catggtttgc ttctatattc attttgaagt
      601 atttatccag attatacttt cttaaaaatc ctaaaacagt agcagtatca aaatcgtatt
      661 gatgtttagt aggctctttt ggcttaggct ctattaagaa ttgtccactg aatcctattt
      721 ccttagcata atctacagcc atttgtaaga atcttgcaaa gttatccatt tcaagttctg
      781 tattagtgtt aagtaaagtt tcataacctt ctcttccacc ccagaataca aaattttctc
      841 ctcctaagta cttagttatt tccatagctt ttttaacctg agctgctgca tatgcatata
      901 catctgcatt acatgttgta gatgcaccat gaacaaatct tggatttcca aacatattgg
      961 cagttcccca taataacttt atacctgtct tttccatatt aacttttata tactcaacta
     1021 tttcatctaa tatcttattt gtctcttcta aatctcttcc ttctggtgct atatctctgt
     1081 catgaaaaca gaaatactca attcccatct tagacataaa ttcaaatcct gcatcaactt
     1141 taatctttgc tatctcaatt gggtctgtta tattattcca aggtctctct actgtcccta
     1201 ctccaaatgg gtcattaccc tctccacata aagtatgcca atagctcatt gcaaatctta
     1261 agtgttcttt catagtctta ttacctacca cttgttcagg attgtagtat ctaaatgcta
     1321 attcattgtc agattttact ccttcaaatt ttatctgtcc tattccttta aatatttcac
     1381 tcatttcatg tcctcctaat attttacaca tataatatat gtagtatgtt ttcttaattt
     1441 aaaactattt tctatcttta tttttctttt taagctaatt taattattta tttttgcatt
     1501 aactacttca tctaataact taaagtcatc tttcaaagat ttgtatattc ttttatacaa
     1561 tctatgatat atattgtatg tatctacatt ttcacttatt ggttctaatt tagttgaagt
     1621 ttttattata gcatcacaag ctttttgtat attttcatat attccagctc ctactaatgc
     1681 aagaattgca actccaagtg ctggtccttc acttgaattt atagtatata catcttgatt
     1741 aaacatatct gcttgcatct gtctccatac tttacttttt ccacctccac ctgaggctct
     1801 tacttcatta acgtctatat taagattctc tattatatcc atacagtctt taagactata
     1861 tgaaacacct tccattattg accttgcgat ttctttttta gaatggattg gagataatcc
     1921 aaagaacaca cctcttgcat aagggtcaag atgaggagtt ctttctccca tcatatatgg
     1981 taagtaaagc aatccattac atcctgttgg tactttactt acttgattat ttataatctc
     2041 atatacatct tcacctaaag aatttgaaac ttccacttca ctttgacaga aattatcttt
     2101 aaaccatttt aatgacaagc cagctccttg agtaactccc ataacatgcc atgtatttgg
     2161 tattgcatga cagaaagtat gaactctacc ttctttatca atttttggct cttttgtata
     2221 agcaaatact acacctgatg tacctatagt agatgaaact actccatctt taactattcc
     2281 atttcctata gctcctgctg cttggtctcc agctcctcct accactattg ttccttcttt
     2341 aagccctgtc aaacttgcta catctctagt cacataacct gtaatttctt gtgattcata
     2401 aactttacct aaaagatttt tatctatatt taacttttct agtacttcat cgctccaatc
     2461 tctttttctt atatcgagca actgcatacc acttgcatct gatacctctg tagcatattc
     2521 acctgtcaat ttgtatctta tataatcttt tggaagaagt attttattag tatttttata
     2581 tatatctggt tcattgtttc taacccataa tatctttgat gctgtaaatc ctgtaagtgc
     2641 tggattagca gttatttcaa tcagtctttc ttcacctact aaatcagtta tctcctcaca
     2701 ctcttttact gttcgttggt cacaccatat tatagagttt ctcaatgttt taccttcatt
     2761 gtccagaata actagaccat gcatctgtcc tgataatcca attcctttta tacattcact
     2821 gcttatattt gaactttgaa ctacttctct tatagattcg actacagctt tccaccaatc
     2881 atttgcatcc tgctctgccc aacctgattt ctcttgtata agaggatatt catatgtaca
     2941 actttttata gtatttccta gtttatcaaa taaaactgtt ttcgtacctg atgtaccaat
     3001 atccacacct aatacatact ccaacttcat ccctccttga atgccacttc ataactatcg
     3061 ttttcctatc ctaatttttt tatattttag gtatatttta cttgctttat ttttgttagt
     3121 atattaaact aacttatgaa ctaataataa aatatttaat tatctttgtc aatgcaaaat
     3181 aaccttttat ttttgtgtaa aaagtattac aatattattt taaggaggaa tatcaaattg
     3241 gttactgata aatacactat tcgagaaatg aatgaaagat tggtacttga acaaattata
     3301 aaaaatggtc ctatttctag ggcaagtata gcttctacca taggattgaa taaagcaact
     3361 atatccgcta ttaccaaaaa acttattgat gaatctctag ttcatgaaat cggaattgga
     3421 aatagtactc atagtggcgg aagaaaaccc atattattag tatttaataa atgtgcaggg
     3481 atttctttaa gtatggacat tggatacgac tatatatttt cttctctatc ttacttagat
     3541 ggaactatta taaattctaa gaaattaaca gatatacaag taagtaaaga caatgttata
     3601 caattgatag atgaaattat aaatagttat aacatatcca aaatagacac tccttataag
     3661 gtaattggtc ttactttggc tatacatggg attacttgtg aaaataaagt attgtttact
     3721 ccatattaca atcttaatga gattgactta tatagcatcc ttagcaaaaa gtatgacttt
     3781 cctattcaca tagaaaatga agcaaactta acagctttag ctgagaatac tttttcaact
     3841 gtacataact cactacttag tctcagtatt cacagtggat ttggttcagg cattataata
     3901 aataataaat tatatagtgg cagaaatggt atgagtggag aaattggtca cactatcatc
     3961 atgcctaacg gtaaactatg tccttgtgga aatcgtggct gtttagaaca gtattgttca
     4021 gaaaaaaagg tatttgaaca gctttcttct ttagaaaata ttcctaaaat agattctgat
     4081 atagtaaagc aactatatta tgaagataac cagaatgcca aaaaagttat tcatgagttt
     4141 tgtagttact taacaatagc tataaataat gctattacaa cttatgctcc tgaaataata
     4201 tatttaaata gccaaataat tagtgatatc cctgagatat tacagattac aaaagatatg
     4261 ttagttagct cttttaacaa aggaataaat atagaaattt catcattagg gtctgaggca
     4321 tctctatatg gtggctctgc cgtaaatatc aagagctttt tgaatataca aaatttaact
     4381 ttaattaatg aaatataagg gttatgacta tatacagcca taacccttta aattatacat
     4441 tctaaaatta gatttattat atagtttatg caagattagc tatatttttt aaaaattgta
     4501 tatcaattaa atattaacat aagtatttcg ttttaaatat taatataatc ttcctactca
     4561 tcatcagata ataaacttgc attttgttca accatagaaa tattatcttt acctattttc
     4621 tctaattttg ctgccaattc ctttgtatct aaaaaagctg attcaataac catacctaaa
     4681 tttagccctg aaataatttt aattcgactg tcattattgg ctactaataa agcagcattg
     4741 catggagttc cacctagtaa atctgccatt ataattattt ctgaactttc cttgtacact
     4801 tctaaagctt tttttaattt ttctattgtt ccttctattc catcatcttg tttcatgttt
     4861 acagtaggat aatctataga atcccctatt atcatttttg cagaatttaa gacttcttca
     4921 gccatattgc catgactcat caaaataata ctattcaatt taatacacct ctttaaatct
     4981 ataagatgtc tacaagataa gttttcttat catcaggcgt cattatagtt gtaatttcaa
     5041 ttccttcatc taaaagtctt ttaaatgctt ctttctcttc ttcagtaaca ctgatattag
     5101 gtcttagaac tgttgtatct tttctacttg acatatttcc tacatttata ttatgtattt
     5161 taaatcctgc ctctacaaat ggaataaggt ccacaggaga cttaacaatt aataatacat
     5221 tttgtccttc atactttcct gcagtaatat tttttatagc agtctcttct gtaattatag
     5281 atgacgctat acctgcaggt gtagccattc ttaaaacttg tttttgaaca gaattgttag
     5341 ctactgcatc atttactacc attaatcttg taacacccaa tttattactc cacatagttg
     5401 caacttgacc atggattaaa cggtcatcta tacgaatatt tttaatacct ttcatttcca
     5461 tatctaatat catcctttcc ttattttaaa atacctaatg catataaaat tattgaaaca
     5521 accattactc ctaaaattaa gtatataggt tttatattct ttttaagagc ccaataaaca
     5581 atcacaacta aaattgcagg aactaaccca ggcataattt ggtctaatat ttcttgacct
     5641 ttcattgtaa aatcaccttg ttgaaagttc aaagcaacat tagcttttac aactgaaggt
     5701 attaatgcac ctacaaccat taaacctaaa atagatgtac tctcagttaa aatctttaaa
     5761 cttttttcaa ttgaagatat tagcttagta cctgatgtat aaccagcatt aaatagtggt
     5821 atacttaata atttcaatgc tacactcact acaatccaca gtaagattcc aaatggactt
     5881 ccatctatcg ccatattagc tgctatagct ccaaaaattg tccacggaat aacgacaaat
     5941 aaactatctc ctaaacctgc tactggtccc atcaagcctg ttttaattga agcaacagcc
     6001 tcttcagtct cttcacttga taactcctcc attgctacat taatacccaa aatataaggg
     6061 gcaacccaag gattagtgtt aaaaaattgt aaatgattta atacagcact ttgtaacttt
     6121 tctggattat ctttgtatac tacttttaaa aaaggtagta ctgcataaca gtatgccaag
     6181 gcctgcatct tctcataatt tgaagaaaca gaactagtaa aaaaccagcg ccataagaca
     6241 ctttttttca ccttttcatt tgatttattt aatgatacct tatcactcat cttcgtccat
     6301 acctcctact acagtatatt gttgttcttg attttccaaa ttctttttgt aaattataag
     6361 tgcaattgct ccaccaatta aagctacacc taataatggt actttcatat atactgctaa
     6421 aacaaaacca attactaagt aactaaaata ctccttagca ggaagatatc taagtaataa
     6481 tcctatccct actgctggaa gtagtcctcc agctactgtt aaaccacctg ttaaccattc
     6541 aggtgtatat ttcaaaatgg tattaattaa agaaggtcca aatattacag atagagcaac
     6601 tggaatacct gttgtagctg ctgttaatag tactcctaac atttgcatat aggttatatt
     6661 tttataattt ccttttttag ctccatcttc tgctttatga actagccata taccaatagt
     6721 atttctaaga acatctaatt gtaccatcaa taaagcaact ggaataccta tagagattcc
     6781 tacagatgct tcttgcttag tcgtaatagt tataaatgtg gctactatac ttgctgtttg
     6841 ataatcaggt attgatgctc caccaaagtt actaatacct aatgataata gctgtaatgt
     6901 acctccaata tataaccctg tatttatatc tcctaagata agaccagtaa taaaacctgc
     6961 tgttactggt tgatatgttc caaacattgt tgaatttttt tcatagttta taaaaaatcc
     7021 atatagtacg attaataata attgccataa ttgtggctcc attatgattt cacctctccc
     7081 aaaatatttt ttagttcttt aaaattataa ctggaatctt gctttaggaa aataggaatt
     7141 tcttccagtg ggcattcaac ttcataagtt ttcccacctt cataactaac tccactaaat
     7201 atattttccc atgtatgtcc ttttggtaaa tatacttttc tacttctatc tttataattc
     7261 attattggag ctactaaaat ttcatctcca agcatatatg tattatccac ctcccaagtt
     7321 ttatcatctt caggaaattc ataaaataaa gttctcataa ctggtgttcc atcttcatgc
     7381 gcttctttca tcagtttttt taaataagtt tttaagctct ctctaatttt tatgaatttt
     7441 gtaagaatta cttcaacttc ttcaccataa gaccatattt cattaggagc tccagttacc
     7501 attgagcctc cacctttatt tgataatggt ttactatgag gcaatctatc tccatgcatt
     7561 ctcaaaattg gtgaaaaagt agcatattgg aaccatctta ccataagttc tctaaattct
     7621 gggtctttag gatttcctcc atggaaacct ccaatatcag tagtccacca tggtatacct
     7681 gccagtccca tatttaaccc tgtatttact tgattattaa aagcctcaaa actagaatca
     7741 atgtcaccag accatactaa agctccatac ttttgagctc ctgcccaagc tcctcttaca
     7801 agagttacaa cagactctat gtcattatgt aaaccatcat aaatcatttt taaataatca
     7861 ataggataaa tattaccaca acttaaaaca tttccttttt tatatctata gttatcaaaa
     7921 tcatacacag aatagccagg ttcagctaca tctaaccaat aatagtcaat cccaagttgt
     7981 ttataatttt tatctattct atcccaaaca tattctcttg cattttcatt tgtcatatct
     8041 acaaagactg tatttccttg aatttgcatt gtcattctaa caccacgatt aacattgact
     8101 aaatagccat tttctaaata atcattgtag ttttctgcat cactttgaac tgtaggccat
     8161 acagatacta ttggttctac tgaatactct tctttcaatt ttttaactaa ttctttagga
     8221 tttttccaat aatctaagtc aaatttatat tcaccttgct taggccaatg aaaatagtca
     8281 attgcaattg atgaaagctt aattcctctt ttagaatatt ccttcacaac atctaaaact
     8341 tcttctgaag ttctatatct tagtttactt tgccatagtc ctaaaaggtt ttcaggcatc
     8401 ataggaacag ttccagtaac ttgagaataa tttttattta actcttttgg gctttctcca
     8461 caagtaatcc aataatctat aaaatttgta gaaaacatct tccattctgt catattttta
     8521 gcaaaagata cttttccaat tcccggatta ttccataaaa gtccataacc tagactagaa
     8581 atataaaaag gaactgatat ttgagaattt ctttgggcta actccaaaac agtattcttt
     8641 aaatctaaaa atttatgttg atattgtccc attccaaaaa tcttttcaga aggctcagat
     8701 tcaaatctag tagttacttc aaattctcca ctatagcttg gtcgatattc tcttgatttt
     8761 aactttagag tagagttaaa gtctttggta atctcaattg tccctacatc ttctccacca
     8821 tcatcatgtt ttacagctct taatcgaata tattctttca ataatatttc atttttatca
     8881 ttataaaaag taattctatc tctatggtct aagacagctt ttatctttcc atttttaatg
     8941 attgcacttc catcttcatt tttattaatt gtaatatcat ctttatttaa cttaggtttt
     9001 tcgttaagag catagttttc atcaacaaaa ttttggtcta caaacgacct aatacgtaaa
     9061 gagttttttc cccatgcttc tattcgcaat agttcattgt caaatctttt ttcaatatag
     9121 tcttcagata tttttatcac ggctacctcc taaatcaatg gtataatatt ctatctttgt
     9181 gtagcttaag ttaaataatg tcgtttagta tcgttattta taaattaggt tttatatata
     9241 attcatttgt acattaaact aacttaccaa taatatactt tatttgactt tgtttgtcaa
     9301 tcgctttccg gattttagta aaattttcta atatgttgct tgaaagtcgt tcttaatatc
     9361 ttttatttat acttttaata tctgtgatga tacctatata catctagttt ttttaaaaca
     9421 catacagaaa taaagtttgc ctattatgta gataatatcg gacttgaaaa tatgtatagt
     9481 gaccttataa atacagataa taatatatta gatattgttc aaaacaatgg attttataat
     9541 tataaataat ttatgaaatt atttaataaa atatataatt gtataccaag tcaaaaacga
     9601 caacaaataa ataaaactaa aaaagatgtt gtctcaaaat atagataaat ttatttttca
     9661 ttgctaaaat gattaaaata tatttgattt aatatacttg atttaatata aaaaaagagt
     9721 gtctcataaa ctaaaaagtt cacttttaga cactccttta tatatattat ctacttctta
     9781 attcatttat tacctttcta cattttcttt cagctagtgg atacatcttt ataagtacta
     9841 attactccac ttaaaaatgg ttcctgtact gtccatcagt catatttttc tgccaaatat
     9901 aatggtgttt cataatgaga caataaatat ttatatctct acttatcttc tctaagctcg
     9961 tcaaatccaa ctataggtat ctctaaatta tattctttaa gtagcttttc tagctcttct
    10021 ataaaaatac ctttcccatc caccatttgt atatcattta ttttatccat caattcattg
    10081 caaccactta actcaccaaa ccaacgttta gaactacatg tcttattata accacaactt
    10141 ggactgccat ctattgcaat gatacttttt atatcataat cattttttaa atattcatca
    10201 aactgcctta catgaggtat taacatttgc ctacattgtt ctctaaaaaa caaattgtca
    10261 aactgttctt ttacatgtcc ccacctattt attccataca taatcatttc tggacatggt
    10321 agttgaatta ttccataccc tttatttatt aaatctattg ctatattttg tgctccctta
    10381 aattctgaca atccctctac tttagaatta caatttaata tacaatgaca tactactaca
    10441 atctctctat ttctcttcat aataatctcc ttagttattc ttaattttaa tgatactaca
    10501 tattttgata attatattat ttatttgttc tatatttcca atattctata tagaaatata
    10561 caattagtaa tcttttataa tctatactat gataaatgta tatatttttt taggaggtat
    10621 tataattgga tactaaaaaa gtcacatttt cttcaatgtg catagttatt aatattgtct
    10681 taggtatggt tatatctatg ataaatatac cattattatt tttagataca gttggtactg
    10741 tacttggggc tgtcgtatta ggtccttttt ggggtgctct tattggtgga tgtacaaact
    10801 tagttttagg tgttatatca ggtcctacaa atataccttt tgcccttgta aatattgttc
    10861 ttggtcttat tgttggatat atttctaaga aaaaaggttt tggttataaa gaagctatta
    10921 ttacaggagt aattttatct attgtttgtc ctttaattgg tactcctatt tcagttttat
    10981 tgtttggtgg tttaagtgga agtggtgctg atttattggt tggtttttta gttcagtctg
    11041 gtcagaagat atttactgct gcgttctttc caaggattct tagtaatata gtagacaaac
    11101 ctttgtcttg tattatggtg gtatatttta taagtaaaat gcctaaaggt tttataaaac
    11161 aatatagtaa aaatacttct agttcatatt aataaatcat ttattatgta aggtttatta
    11221 ctctttaaaa tgctatatac tcatttttat ataacatttt tattaataat ttaattagat
    11281 agtatttgta ttactttaat taaacgtaac ctcttgatat aaatttgagc tataaatcta
    11341 tatcaagagg tgctatttta tttttaatat aattaatatt ctattacgca aaaaatgcat
    11401 atatttcatt attttatgga aaatattact tcatttaact ctatatcaaa ctcattaagt
    11461 aaaaatctaa ttatatttaa tttagcctct gtatttaaat ttgtttctaa atatatattt
    11521 tctgatattt taataccttt tcttaattta tatgcatctc tagacttaat attatctata
    11581 aaatctcttt tataagtttt gtctacaata ctgttaaacc ttacagaatc caaatcatat
    11641 aaagtaagag caacttgttc ataaaattct gtccaatttt tagccttata tgactctcct
    11701 aatatactaa atgatactat actttcattt gtaaaattaa catcatcatc ctctaacgta
    11761 aatatatttt cactgttact tggaggttca tatgtaacac ttggatattt ccaaatttct
    11821 acagctctat ctagtaatat cttagttcta ttttttattt cttcttcatt ccacttatct
    11881 atagatgaaa tatatttatt tagataaagt ctactatctt taaatccatc ttccatttct
    11941 ttcttttctt caaatgtttt attacttagt tttgaattat atcctgtcaa agttaaattt
    12001 ccaattgtat gtaaatattt atcatgtatc tccttatagt tatctcctag tgattccttc
    12061 catttatttg ttaatgtctg tggcattata tgctctatat ttagcatatt attattgatt
    12121 aaattttcta aatcaacaat ttctttatta ttataattct ctaacctctc aagtatatat
    12181 aacttattct ttgacttagt attatatata tctttagttt caaaagcatt tgaaaattca
    12241 ctgtcagtag gaaatctttg agatgatttc ttatttataa aaatatatct taatatatta
    12301 gaatagtttt ctttataatc actaaatttt ttaatttccc ttcctaagtt cataaatact
    12361 ttattcagtg cattaggagg tacatcacat actattcttc ttaatataaa acattctact
    12421 aaattaaggg attctacaag tactttttta tttattatac cttcattata atcatcaaat
    12481 aattctaata taaacggata tgatacaaat atttcaagtt tgtttatcct tttcaaaact
    12541 ttgtttacat cattatcatc agtacatgaa taagatatct ctctataata gttagaaaat
    12601 ttaagtaaat cttttaataa aatttctata tctataccat tatctgatac atacctttta
    12661 aaactaaaat atactttatt tttattcggt atacttctct cctttatagt caaatagtct
    12721 cttataaaac tacttacgtt gctacttgta tttttttcaa ttttatacca ataattatta
    12781 taaaattcct cttgtaactt agagcttcta cccattaaca caaagtttct tactctatca
    12841 gcttcactta aatctaagcc agttgaattt agactttcaa agattaattg agggtcatct
    12901 tcaccatttt ttaactctat ttctactata gttaatttct gtatagcatc aaacaaatca
    12961 tctattgata tattatcttc ttttattcta ttataaaaat ataaataatt tgaaactata
    13021 ttagaatttc tatctgtatt catttcttct tcaaatagtt catagtacgc ttttctatct
    13081 tctgctattg gttttaactt tatttttcca tctttatctc ttgagtgctt gtttataaga
    13141 taacactcct taatctgttc ttctgttgta ataattcctt ttgattcccc aaactctttt
    13201 attatttgac acatggcaat caataatatt gacacagtag tcactctttg ttgtccatct
    13261 attattaaat attcttttcc catactatat atagatacaa ctgaccctaa gaaatgagtt
    13321 ctaaatccag aatctattat atcctctaaa tcatcaaaca atcttttaca ttgttctttc
    13381 ttccagttat aatttctttg atatacaggt actacaaaac ttttgtttga cccctctaaa
    13441 taactaagaa attcaatttc tcctgctttc atattaactc cccctataag ttaaataaat
    13501 tccattcata actccatgta tctatatatt atatcaaaat tttgactcat aaatgtatat
    13561 gaaagcctta ttatatacct ttttatacct gtttttattt tattaaagta aatacatgat
    13621 tattctataa aatatacaaa tacttaattt taaaaattat tatttaatct tcttcatgag
    13681 taattcatag ttatttatca attaaaaaga gatattgcat aattattcaa tacctctctt
    13741 tgtatattta cattatccat atctagttat ctatttcaac ccaatattag actacctact
    13801 aataaattta ttctctacat cattaacaat aattttgcac taaataacat cattagccta
    13861 tctctgatgc attttcttta attttattta tagttttaat caatttattt ctctcttcaa
    13921 tatgattttc aaataaatca attatacttc caacagttct aaatgggtct tccatcaaca
    13981 ctttgttatc ctctataaat ccattcttga ctacataatc gactataagt ttaacaaaat
    14041 gtatttgctt agtattaaag ctcttattat ttaaaaactc tgaaaaagct tcatttgcag
    14101 cttctctatc caatcccact agtcttctca ctaattggct tactggagta tctccaaact
    14161 ccttcttata atcatcacaa ttacctaact gttttaagag tatttcctcc aacgtttcaa
    14221 catcttgctt tgttatcttt ttattatttc ttaatttaaa tattgctaat tcatctttat
    14281 ggctatttaa ataatactct accttctttt tataattctt aagattgttt gcattatata
    14341 cagaatcatt tctatcttct gctacaatca tatcttcaaa acttgtataa tatatctttt
    14401 gactagactt ttcaagatac ttaataagct ctcttaatga tatacgtact tcttctacct
    14461 caaatatatc tgaatcttcc caaaaatcct ctgtcattgc tttttcaata gtatattttt
    14521 gttgctgaat ctgtggtata gtcccaagtt ttgaaagttt ttctgctgta gccattacat
    14581 gccttatacc tttacttgca ttattatttt gtaagtatgc aagttgtata ttatacataa
    14641 ctacatcaaa tcttttagcc aattcatcat cactatatga agttatgagt ggtgatatat
    14701 attctcttat ctcattttgt gctactgctc caatattgct ccaaacattt tcatttttat
    14761 acttatgtac ataacttaaa ttcatcctaa ctatataact atcctcattt aaagctttta
    14821 cagaagttat aagttcttct aacaattctt ttctaagttc tgcatactca ccttctatat
    14881 atttaatatc ttgaagttct ttaaccatac ttacctttat attaaataat ttttcagtaa
    14941 gtgtttctgc tttatttccc tcaaatcctt taggattaaa tttaaaaaat tcaaaattat
    15001 tacaaaaatc aaatattaag aatctctcct tatccatatc aacacctaat aaatctggac
    15061 ataatctagt tcctctacct atcatttgcc aaaacttagt cttacttctc actttcttga
    15121 aaaaaacaag atttagtatt tcaggtatgt caattcctgt atcaagcatg tctactgaaa
    15181 ctgctatttg aggtaattta ttcttatcag aaaaatcatc tattatagaa tctacataat
    15241 ttatactata gtcaactgct tttacaaagt tcccaccata ctttggatac agcttattaa
    15301 atctttcaac tattgcccta gcatgctttg tattctttgc aaaaattata gtcttaccaa
    15361 tcttttcttg tccttctatt ttcaacccct ctgtcattag cttatttaaa actaaatcta
    15421 ttgtgtcaga attaaatagc cactcattta ctgcattatt ccctatttcg tctccaatac
    15481 tctcatcatc attaaatgta ttttcatatt cttccttctc ttcatcagaa agctcatcat
    15541 acttaattcc atcttccata atcttagtct taaactcaat actagtataa tctactaaat
    15601 atccatcctc gactgcctta ttatactcat aagcatatgt aggtactcca ttttccatgt
    15661 caaaaactga ataagtattt ttatctatct catcctttgg tgtagcagta agacccatta
    15721 agtaagaatc aaaataatca aagattgact tatacttttt ataaatactt ctatgcgatt
    15781 catctattat aattaaatca aaatgaccag gtgtaaacaa tctctttcca tccttagact
    15841 tcgtatcatc aattgcattc atcattgttg gatatgtaga aaatatcatc ctagcatctt
    15901 ctgggctatc tttgctatcc aataaattac ataacgccaa gttaggtaat agcttagtaa
    15961 aacttttttt agcttgcttt accaatgctt ttctatctgc tagaaataat atatttttaa
    16021 tccaattatg tcttgaaagt acatcaacta tagatatagc aattcttgtt tttcctgtac
    16081 cagtagcaca taccaaaaga gttttgcgtt gcttattttc aagagcctca catacagccc
    16141 taatagcttc tttttgataa tatctattgg atatttcatc atttattgtt acagaagtta
    16201 aagacttctt actctttctt ctatctatca taagttgtaa ttctgatttt ttataaaatc
    16261 cataaacttt cctatctgaa tagtcatccc aaatatacat atcaaaacca ttagtcaaaa
    16321 atattaccgg tctttgacta tatctctttt ccaaacaatc tgcatataat ttagcttgtt
    16381 gttgaccaat tttcgggtct ttactcgttc ttttcgcctc aactactgct aatggcttac
    16441 cattttcacc ataaagaaca taatctacat atcctacacc actttcatta ggcatacctt
    16501 gtacttctat ttcttcccat atatcttttc caaattccca acctgcaagc tttaattcta
    16561 tatttatata tctttttctt gtctcaaatt cgcttacttc gtctatcttg aaatcatagt
    16621 ttgcagtatt aacttttctc ttttcagtta gtgactttct taattcctca ttttctttta
    16681 ttacttcttc aagcttttta tcttttgaac ttaatctctc ataaagattt ttaagttcgt
    16741 ctggtctcgt ccttttctct tccccatgaa gtaataaatc ttcattaaag tcttctgctg
    16801 tatattcatc tgaataacaa tagtctatcc aactaacaaa ttgatgcaaa ttgttaagtg
    16861 aaagtattgc ttctcctctc tctatgttcg cactagtatg tacagataaa ttacctaatt
    16921 taactatata ttttaataaa ggtaacatat catagtctat aagttctata aaactattat
    16981 tatgaatcaa cgtactgaga ttatcttgat aaggcaatac taaatcacta tcattagcat
    17041 atagccattt tactgcaagc tctaaagctc ttcttgttat agttgcacaa gtagatggac
    17101 taactagtat acttttttct gcttctaagc aagcagtatt aaaactattg aaatttttct
    17161 tcttttgtaa aaaatcaaaa ttacccaagc ttttatcccc cttttgtccc ttagttaaaa
    17221 taaatctctc taaaaactat ttcttacagc tgtttcttat actaacaaat acttcttcat
    17281 tttctgtaaa caattcgtac atatcttcaa actgtggtat atcatatcca tgagaatcat
    17341 catttctcaa ttttctaagt gtttctataa ctatagaaga atttttaggt aataaattgt
    17401 gatactcaca agctaaaagg tagtcataaa agtttttaga ttttatgttt aaatcaattg
    17461 atttataata caacattgct tttgttaatt ctagaaatga taccatatag cttttaaagc
    17521 cttcttttat gtatctagcc cataactcag gcatttcttc cttatcatat aagattttga
    17581 agttttgcag gtcatacaag caagaaccaa aatccctatt tacttcttct attgagttcc
    17641 aagataattt gtttctaatt accatacatg tccctccaat ctaagatatc tattacatca
    17701 ctgttttcaa aatagaattt atttatataa tccttaaaat ctttagattc ataaataact
    17761 tcaccttcta aaatagcttt tttaagtgct attgtataat tatctgtaac aatttttagg
    17821 tctacatcaa tgtctaatat atttcttaga gcttctgtct tagttgcaag ttctgtaatt
    17881 ggtacttttt tgataggaat ccaagctatg tctatatcac tttctgatgt atgtttatct
    17941 gttccataac taccaaatat tatgattgct agagtatttt cttttggaaa tacatttatt
    18001 attgagtttt taatctcatt tggtatattt ctcatttaat cacctcttgt ttattaggta
    18061 atattactta attaaattta tatccataat cttttaatct atttatacat atttctcttt
    18121 cttcatcagt aaacaactct ttatactttt cttttaatac taatacttct aatgataaat
    18181 ctaatctctt acactcccat agtcttgtaa atccttcagt agctccttct ttattgatta
    18241 attctttagc agctaataag gctcctttgt tatttatcag ttgtaaaaat cttgttggct
    18301 tatagttaca ttgtaaatca gcttctttat agatatttac catgttatgg tataaatcta
    18361 gctctatttg attcatagat tttattcccc cattttaata attgtatttt agttgaataa
    18421 ctctccttta aaagcttttt gcattaatga gttaaagttg tcttctagtt cttttaagct
    18481 agtttccatt tcaaacttca aagagtttac ttgtttaacg aagttggcaa attgattttg
    18541 aagtctaata ggaggtacaa taatatttat ttttctaaca gcatctttag ttatcgtttt
    18601 tgttacagct cctcttactt tctcttttat tttactttcc atataggatg ttagtaaaag
    18661 gttatataag aatatatgat tcactttatc ttcattaaat ctaagcataa attgattcat
    18721 agctaaagat actggtttat ttaaaaatgg cattaaataa actttacctg cactacctat
    18781 cttatttatt ataacctcac caccaaacac ctttgatttt tcaagataat tatatgcatg
    18841 ttcatcgata tacttaacac ctttttcaaa gttattattt tctaaatctg tagttcttac
    18901 cattaaggca taaccttttg tatctaaaag agttacatta tctcttaagg tttcataact
    18961 accatttgaa tgataatcag ttaaaacatc taaatatttc cctatttctg aaatttccca
    19021 gtttttttca ttctttgatg gagtaccaaa catctcaata aatcttgatt taactaattc
    19081 atccaatact tctatttgct cttttctttt atctattaac tcttgagcct tgtctaaaac
    19141 acgcactacc ttttcttgca cttttaaatt atacatgcgt atttttatat tagctaatcc
    19201 ttcattattt aaagctgatt gtgtagcacc tttacttaac ctattttttt gatttagaaa
    19261 ataactacta tttaagtaat ggtataaata ttgaggcaat atttcttttg atggttttaa
    19321 aacataaaat ccagtcgata ctattatatt tttattagtt ttattaataa ctaatgtttt
    19381 ctttgtgtcc ttcatttttg caaatataac ttctcctata tttacagttt gatttgctct
    19441 agaaggttta ttttcataag taactatttc tagattgctt atcttgttaa aatctaagtt
    19501 tccagtacta agatatgagc gttctccatc aaactttttt acattttccc cgcttatact
    19561 aacaacatct aaaatttttg cttcatcaac catactctac acctctaaca ttctttctag
    19621 ttcctctaat ccatccacta tctctttctc taacgcctta accctaccca taataacact
    19681 aggagcatca taaactactt cttcatacac tacctcttta tacttattaa tagataaatc
    19741 atacccattc tctcttatct cttccacatc aacaaaaaaa ctttgctctg ttctttttct
    19801 atctacttca ccatcaagat tatcaaatct cttaataata tcctctatat cactatcctt
    19861 tatctcattt ctcttatcat ccaaagaata accatcagct ttcatatcat agaaccatac
    19921 cttatcagtt ccacctgttc ctgttttagt aaatatcatt atagctgtag atactcctgc
    19981 ataaggctta aacactccac ttggcattga tattatagct tccaatttat gattatcaac
    20041 tatttctttt cttatcgatt tatgtccacc tgttgaacca aacaatactc catctggcac
    20101 tatacatgcg catcttccac caatttttaa aactcttaaa aaaagtgcca agaataataa
    20161 ctctgttttc tttgtcttag tcacttttaa taaatctgct gacactgctt cataatctaa
    20221 acttccctta aaaggaggat ttgctaacac caatgtaaac ttatctttat ctgtattaac
    20281 ctccgataaa gaatccttat actctatatt aggattgtca acgccatgca acatcatatt
    20341 catagcacct attctaagca tagttctatc catatcaaat ccatagaaca taccattatt
    20401 aaaatgctcc ttcagccctt gcactaagaa aagacttgaa tgattatccc ttaagtattg
    20461 ttgtgatgat actaaaaaac ctgctgaacc tgctgccggg tctactataa tatcttctgg
    20521 tgttggttta ataagtctta ctatcatatc tattatgtgt cttggagtcc taaattgacc
    20581 attagtacct gctgtagcca ctttggataa taaatactca tacaaatctc cctttgtatc
    20641 tctatcctta cttaattcta aattactaat accatcaact attttagata acataagagg
    20701 cgtaggaatt ttaaatattg catctcccat gtactttgca taagctgagt ctttatttcc
    20761 atgtaatttt tttatgaaag gaaacacttc attttgtact atttcataca ttttttctga
    20821 atttccttca ttagaaaact tactccatct taaatgttga tgttcatcat taaatatcct
    20881 ttcaaaagaa actcccaaaa gagttgcttc tgcttcattt tttgtttcta cttcatccaa
    20941 tcctttaata aataaaagat atgtaaattg ttctataact tctaatggat tagttaatcc
    21001 accagtccaa aatgtctccc aaatcttgtc tactttgctc cttaattctc ctgtaatcat
    21061 atatgacttt ccctccagtg ttcataaaat aatttaaata tatattttag tcgtacgcct
    21121 taatagttag tgtcttatgt taaatccaag tttttactac acagttatta aattattttt
    21181 cctatttaca tgttttagtt aaaaaactaa tgcaatactc ttattttatg a
//
LOCUS       DAELNY010000055        21218 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.055, whole genome shotgun sequence.
ACCESSION   DAELNY010000055 DAELNY010000000
VERSION     DAELNY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.055"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            420..578
                     /locus_tag="KPE55_001719"
     CDS             420..578
                     /locus_tag="KPE55_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492805.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(865..1569)
                     /locus_tag="KPE55_001720"
     CDS             complement(865..1569)
                     /locus_tag="KPE55_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0492806.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1560..3167)
                     /locus_tag="KPE55_001721"
     CDS             complement(1560..3167)
                     /locus_tag="KPE55_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG0492807.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3421..4599)
                     /locus_tag="KPE55_001722"
     CDS             complement(3421..4599)
                     /locus_tag="KPE55_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492808.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4825..5319)
                     /locus_tag="KPE55_001723"
     CDS             complement(4825..5319)
                     /locus_tag="KPE55_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0492809.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5320..6081)
                     /locus_tag="KPE55_001724"
     CDS             complement(5320..6081)
                     /locus_tag="KPE55_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0492810.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6783..9617
                     /locus_tag="KPE55_001725"
     CDS             6783..9617
                     /locus_tag="KPE55_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0492811.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSIVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9660..10904
                     /locus_tag="KPE55_001726"
     CDS             9660..10904
                     /locus_tag="KPE55_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0492812.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(11319..13061)
                     /locus_tag="KPE55_001727"
     CDS             complement(11319..13061)
                     /locus_tag="KPE55_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492813.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(13045..13719)
                     /locus_tag="KPE55_001728"
     CDS             complement(13045..13719)
                     /locus_tag="KPE55_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492814.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(13756..14745)
                     /locus_tag="KPE55_001729"
     CDS             complement(13756..14745)
                     /locus_tag="KPE55_001729"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0492815.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(14793..16037)
                     /locus_tag="KPE55_001730"
     CDS             complement(14793..16037)
                     /locus_tag="KPE55_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0492816.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(16079..18625)
                     /locus_tag="KPE55_001731"
     CDS             complement(16079..18625)
                     /locus_tag="KPE55_001731"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492817.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(18833..18994)
                     /locus_tag="KPE55_001732"
     CDS             complement(18833..18994)
                     /locus_tag="KPE55_001732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492818.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(19050..20066)
                     /locus_tag="KPE55_001733"
     CDS             complement(19050..20066)
                     /locus_tag="KPE55_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0492819.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPEID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(20063..>21218)
                     /locus_tag="KPE55_001734"
     CDS             complement(20063..>21218)
                     /locus_tag="KPE55_001734"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0492820.1"
                     /translation="ENKIKIYDFVNYFDDESDKKYIINLIDIMNKNQIFKSNNLNFDM
                     LSNGVAIYFCLTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITG
                     GEPLIREDFFEIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDE
                     NTCSETRGKGVFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEF
                     VERYFIPTGRGLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNS
                     QIFINYDGDVYPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFE
                     KCKDCDVNIFCWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
BASE COUNT         7350 a         3423 c         2231 g         8214 t
ORIGIN      
        1 aaatataaaa ctattatata ttaaaatttc ttcaataata aattatctag attgttactt
       61 tgatatatta tataatatca caaaggctga tataaaaaga caaaaagggc aattttttta
      121 tatcaaaaaa aaatgttaaa ttgatattat ccatatataa attgatatta tccatataga
      181 tgctatattt aacaattcta cttacttccc ttacacttat tagcatatag ttattaagag
      241 tttgttgcaa aacaacactt agcacacttt aaaaagtgtg gtctaataag taatcaacga
      301 agaagacata ataaagcata aaatatgata tacttttcca cgccatcaaa actgaggatt
      361 ttattattaa tatatgctaa aatatgctta ataaattaaa ttttaatggg agaatagtta
      421 tgaaaatatt tagtttcttg ttgtttgtga taggaatatg tttatgtatt gctggattta
      481 taggacatta cccagtattt gttggtacaa cattgagttt tcttgcaatt gcaatgatag
      541 ctgtaagaca agtcaaaact aagaaaaaaa tgaaataggc aaatcctaac tttacaagtt
      601 aatattacat aagtaattag tctaaaattg gatacttttt ctattacaga aatacatcat
      661 ccactttgta ttttcttaaa aaaataaaat agctatgtaa attcaagatt agatagatta
      721 actctctatc catttcattg taaaatatac atagctattt gtattcgatt taaaatactt
      781 agattttctt acataacaaa taatttctat ctgattatta ctcatattca tttagtaatc
      841 actcagcaac taatcattta gtatctattc atccgccata cggtaaccta ccccaacctc
      901 agtcaaaata tattctggct gagcaggatt cttttcaatt ttccttctta tacttgccat
      961 aaaaactcta agcgactgat tttcactttc atagtaattt ccccaaactt tatctattat
     1021 aaacttatgg gttaagactc ttcctgaata cttagacatc aactcaataa tcttatattc
     1081 gattggagtt aaatgtattt cttcaccatt aattgacaca atatgatttt cataatctat
     1141 ctttaggttt ttaacttcaa aagactttgg agcatcatta tttatattat cctgttgagc
     1201 tttatttcta agagccacac gtactctagc caaaagctct gttacactaa aaggtttagt
     1261 taaataatcg tctgctcctc catctaaagc ttccacctta tccctctcat gctctctcgc
     1321 tgaaacaaca attattttat tagtctttgc acatgcccta actcgtccta ttacctcaat
     1381 cccatcaata tctggtagtc ctaaatccaa aattatcaaa tctgggtcaa gagaatgtat
     1441 aagtgatata gctgttcctc cattatctgt ctctacacac ctataatttt gagtttcaag
     1501 agatactttt ataaatttac aaattggctt atcatcttct acaattagaa ctaatggctt
     1561 tatttccact tgtctttccc ctcctcatca tacataggta tgctaaatct aaatgtagct
     1621 cctccagaag gattattaaa ggcgacaatt tctccaccat gagcttctac tatagattta
     1681 catatctcca atccaagacc aatacctctt ctttcaagat ttctagactt attcttagta
     1741 taaaacctat taaaaatatt cccgatatct tcatttggta ttccttttcc attgtctgca
     1801 acttcaaata gtacttgatt tgactctttt tttacagtta gttctatctt agaattttta
     1861 ggtgtatgcc ttatagcatt atcaattaga tttactaaca cttgttctat taataataca
     1921 tccacatgaa cgattattat ctcatccgga atatttgttt ttatgtctct actattggca
     1981 aactttttga ctcttaatat agattcagct attatttcat caacaacctc tggtcgtttt
     2041 tcaatatcca attttccttc atctattctg gtcatactaa gtatattctc aacagaatga
     2101 gttagccaac tcgtatcttc atatatattt ttcaataatt ctactctagt ttctttatct
     2161 atcacatcat cattttcaag tattgttgaa gatgaaccca atatacttgt aagtggagtt
     2221 cttaaatcat gagaaattga cctaagcaag tttcctctta acctctctct ctctgcctca
     2281 agatttgctt ttttattttt ctcaaataaa ttttctctat caattgccaa cgcaacctgt
     2341 gttgaaactg cttctaatag tatcttttca ttttctgtaa taatatctat cttaaaacac
     2401 gcaacaccta taacaccaag aacatttgat tcttgacctt ttatagggaa ataatatgca
     2461 ctgctgtttt tacaatattt tgtcccaatt ccaactgggt tacctgtcct aaaagcttct
     2521 tttatagctt ccttttctaa tactgaatgg aatatattct cacttccatc attgttacaa
     2581 atataaatac ttggcttacc tagattatta ttctcatctg caatagtcac tattacagtt
     2641 ctatttaata tatttactaa attagaccca caaaattcaa taatttgatt tttatttttt
     2701 gctttaagca acgttttgtt attctcatag agcaatctta tcattttttc tctcttatga
     2761 gaaagtctca cttctctttt tatcctagaa gttaaagtac ttgttataat tgctgccatt
     2821 agcataatac aaaaggtcat aggataatct tttctataag ctaaaaaagt atataatgga
     2881 tgcgtaaaga aaaaattaaa tgatagcact cctattactg aagaaagtat tccaaaaaaa
     2941 tatccatagg ttatacttgt agcaaatagt acacctaata tgtatactag tataatatta
     3001 gattcattaa aacctacctt ttcaaaaact actgatattc cagtagcaat tgtcataacc
     3061 aatatagttt ttatgaagtc cttaataatt atctttttac tgagatagcc atcttcatca
     3121 taatcatact cttttaagtc ttcatgttgt agtcgttcct ttttcattta ctacaattcc
     3181 tccttattgt tactgtttaa attggctcta ttttatataa tacaatttta tcataataaa
     3241 ttcaatcaat acatttaatt aatgtactta aattactaaa aggtgctgtc ttaaaatgaa
     3301 aataaatctc tatttcaaaa cagcacctat atatttaaat tactaattta gtttttacat
     3361 acttaaatca atagttttat tcaatttcat aaattataat acttcaatta taaaacattt
     3421 ctatccaaca agcataaata taaagtgggc acatatacct gcacataaac cacctatagt
     3481 atgcgaaagt atcgctttac cagccaatgg tcttgcatct aaagcatcca tcatacctat
     3541 gtgtgtactt aaatatccac tccaacacat acccattgct gtgaatactg ctatatcatt
     3601 aggagttata gcgcctgatt ttataaactc tggtactaaa gatattgctg ctccaacagc
     3661 tcctaatgca gttacaggga atgctattgc ctctggtgat gtgaatccaa ataatggttc
     3721 tattatgaag ctaatcttat caccaatagc aggtaataat tttatacctt catatgcagc
     3781 tcctgtgtaa acagcttgac ctgtagcagg gtctgtagat ggtccaaaag ttaataacat
     3841 aactaatgta caaactacta aaactcctgg tattatagcc atacccattt caacacctaa
     3901 cttaccacct tcaagtatag catccaatgt tctttggaat acattaccat ctcttatttc
     3961 tctaaactct tcaagttttg cacctttatc atttatttgc ttttcagtat catttttagg
     4021 gtcataatta taatactttt tagtaaaagt caacataatt ctaacactaa taatactacc
     4081 tattattgcc gcaacattac ctattatagc tggtagtaca tattctttac cttgagctat
     4141 cataaaagtt gttacaataa gtcccatacc aaaggctgtt cctaaattac ataaagcagg
     4201 tacttgatac ttcttaaaat attgtgtaaa tgttttgtct ttagcaaatg gtataatagc
     4261 tggattatca gataaatatg ttgcaactac tcctgctata ctagcaccag gtaagcccca
     4321 tataggtctc ataagaccct taaatatctt attaactaaa gatattactc caaactctga
     4381 aagcaatgca ctaagtgcac ctgcaagaac tgccatagcc attattaaaa atactgtgtc
     4441 taataaaagc gcatgtgctg tactcattat tactttaaac atcattcctg cacccatgat
     4501 gcttcctaca tatccaaatc ctaccgccaa tagtacaata aatacgaagg tttccatact
     4561 tactgccttg actcttctct tatccgacag cttttccatc ttgaaatctt ccccctttta
     4621 attttaaatt aaggttccta acattggtac aaattttttg gtacaaactg agttctaaaa
     4681 cttacactcg taatgcaagt tgtcagtttt ttgaaataaa aaaaaattac gtttatagaa
     4741 taagaatatc ctctttaatt tggatactct tattctattt tatgttatta tattatcatc
     4801 cttttgttta aaagtcaatt ttttctattt ttgttgttta attttattta tactcttttt
     4861 atatttttca ttttctttta tctttgtaag tactccatat aagttgactt ttctaaatac
     4921 agtagttata aataccaatc ctaatgcaat tgaacttgca cacgataatg tactcacccc
     4981 atattctaca gcttttatat aattattggt caaaacagaa tacatagtat tatatattcc
     5041 ataacctggt actaatatta ttaaacagca aacacttaat gtagttgctg gagtcatata
     5101 aattcttgca cataactcac aatatatcgc aaaacaaact gtagctataa aaaaacataa
     5161 gttttctgtt actcccatat ttaaaagaaa tttatacgaa aaccaagcta tacttccacc
     5221 tattcctgct agtattaagt tttttccttt catattaaat agcactccaa agcttaaggc
     5281 agtaaaaaaa gctgctatta cttctataaa caaatgcact tatatccccc ctaacttgaa
     5341 gtacaatcca agtaccaaga acattcctac tgctatagat acacctacaa aaaatacttc
     5401 tgctaatttt gcaagaccag aaatcaactg tccatctaat aaatctctta atgcattagt
     5461 caaagcaagc ccgggaacta agagcattat tgctcctgct attaaatggt ctaacgtctt
     5521 tatgaatccg agctttaaaa ttaaaattga acaagaagct ataataactg ctccaattgc
     5581 atttacaaaa aagtcattaa gggaagattt acttaaatac ataataacaa acttagtcag
     5641 aatcccaaca gtaaatgcag ctataaattc ttccacattt cctccaaata atatcgaaaa
     5701 agttgctgct gcaatacctg caaaaaatat tgttattaaa tccgagtaat agttatcttc
     5761 acttatttca tctaaaattt tctcacataa ttcaatatct aaattattta tacttattgc
     5821 tcttgataat gagttaactt catgaaccaa gtttaggtct actcttcttg agcttattct
     5881 tttaattgct gaatgtaatt tcccatctac aaatacagat gccattacag ctgttggagt
     5941 tgcaaataca tctacgtgat ctattccaaa agattgacat attctagcta tcgtttcttc
     6001 aactctatag gtttctcctc cactttgcaa catagcttta cctgcatttg aggaaaagtt
     6061 taaaattcta tctatatcca caaaacatat ctcccattct tttatagtat tattcatatt
     6121 ttaatacaag atattattta tttaaactat tttaaataat tcatatccaa tatttacatt
     6181 ttatgaaaaa acttgttttt tatgcttgtc aaaatcaaat tttaagcttg tttacaccat
     6241 aaaatggctg aattaatatt tgacaaacac tttcaaccat ttatataacc ttctaaattg
     6301 tataactttt tactacaaat aaaattatat ctcttttaat tctttctctt gagtatttag
     6361 cttatagtaa cttcatattt atatatgtac aatataaaac attattttta tgatacaaaa
     6421 tataatttgt aaatgtatat atgtattaac tgtgtatata taaattattc ttaaccttat
     6481 cagttttttc atatatcttc atctataccg accatatatt aataaatgat attacataga
     6541 cttaagtaca ttaaaaaact taaatttagt tatttaacaa cataatactc taatttaatc
     6601 tataaaaatc catacctaat ataatgcaac tcttatttta acaccatcta ttacacaaaa
     6661 caacatatta aaaagtaaaa tacaaaatat ccactataaa ccttatttta atactgaata
     6721 tatattataa tatatttaat gtagcttaaa tatagtttaa acataaagaa aggaaacggt
     6781 atatgctaag aaaagataaa gttctattat ctctacataa attatgtgaa aatatgactt
     6841 taaaagacct tcaatcagat aaaacaggat tcactacaat agaaattgct aaccattcat
     6901 ctcttgatag aagtaatacc agtaaagaat taaataacct atacaatgaa aaaaaagttc
     6961 tgaaaatatc tggtaagcca attttatttc tacatgctag tattatagaa aatttaattg
     7021 gccgtacttt gaacagtgat gaattttcta taaataattg taatgagcta cttaaatcaa
     7081 gtaaaaatga ctcagaagat attttttcaa cattacttgg acatgattct agtcttaaac
     7141 tacctataga aaaagctaaa tctgctatac tctatcctcc aaaaggtctt aatacgttac
     7201 taataggacc tactggtgtt ggaaaaagta catttgctga aacaatgtac aaatatgctg
     7261 tagaaagtaa aatcttttct tctgaaagca aattcgtagt atttaactgt gctgaatatg
     7321 cagaaaatgc taacttatta ttaagtaatt tatttggtta tgtcaaaggt tcttttactg
     7381 gtgctaataa ggataaattc ggcatagttg cacaggctga tggaggcata ttattcttag
     7441 atgaaataca cagattacca ccagaaggtc aagagatgct ttttcttttg atggacaaag
     7501 gcatatacag acgattagga gaatctgaca ttgtgcataa agctaatgtc tttattgtgt
     7561 gtgccacaac agaagatata cattcttctc tgcttgggac ttttttaaga agaatccctg
     7621 ttacaatcaa cctacctgct ttacaggata ggtctttaaa agaaagatta tctcttatta
     7681 aatacttctt tattatagaa tcaaaataca ccaatgcaac tataaaagtt cacaaagatg
     7741 taattaaatc attactcctt tatgattgtt ttggtaatat aggtcagctt aaatctgaca
     7801 tacaacttat gagtgcaaga gcattcttaa attacaaaac tggacttaag gataatatag
     7861 aaatagattt atccattgtt cctgattaca tctatattgg tcttttaaaa ttgaatgaaa
     7921 atcgtagtaa tctaaataaa ttatcagaac ttgattatat gcttttttat gaatttaatg
     7981 gtaataactt agaagttgaa tatacaaaag aactatctaa atacaatata gatacatcaa
     8041 acaatggatt taaatactct acagagattc cttatctgga aaatgatata aggagtatgt
     8101 ttataaatta catggaaaag tactctacaa aattactttc gatatcttca gtaactcatt
     8161 ctgattatac aagtaatgag atttttaaag taatcaatcc aaagatttac tatgctgttg
     8221 aacattcttt aaaagaggct gaaaaaatat cgagaaacaa atacacaaaa caaacttata
     8281 ttgctctttc aatgcatata agtgcactta tagaaaatat aaatcaagac acatataatc
     8341 ctaagtacaa tttatataat gataaaattt tcttagataa agatttaaat atagctaaaa
     8401 tagttttaag tataattgaa aatgacttac aaacatcttt acctaaagat gaagtaaaat
     8461 ttatatccat gtttctaaat agcatgtatc ttgaaccaaa caatcaatct gaaagtatag
     8521 gagtcataat acttgctcat ggaaattcaa cagcaagcag tatgtgtgat gtagcaaatt
     8581 cattgcttgg tacaaaccat tgtaatgcaa ttgacatgcc tcttgatgta aaagtaagta
     8641 ccatacttga taaaactata gagttggtaa aagaatgtga cgaaggtaag ggtgtacttc
     8701 ttttaacaga tatgggctct cttatagctt ttggagaact tatttcagaa aaaacaaaaa
     8761 tcaaaactcg ctcaattgaa atggtatcta ctccaatagt tttagaagca gtaagaaaaa
     8821 gtacactacc agaaatgact ttggatgaat tagctaattc cttaagctct ttaaatccat
     8881 atattggacg agcagcaatc gattcaacag aaataaacta tcctgaaaag tcaagagagt
     8941 atgtaataat aactacttgt ataacaggat atggttcagc tatgaaagta gcagacttta
     9001 taaattcttc acttaaaaat attagtaaat acaatattaa tttagttcct cataatatag
     9061 aaagttttaa aatgtataag aataaattta gtaatactaa aattctggca gttgttggaa
     9121 gtgtagattt taatgtagcc aacacccctt ttatacacat agaggaacta ctaaatggat
     9181 atggcctaga tatgctaaac aaaataatag aaggtacatt caatgccaat agtattacaa
     9241 atataaaaga aacaaatcta tcatacaaca gtttaataat aaactttatg acccaaaact
     9301 tagagctttt ggactctgtt aaattattca actatgtcaa ttcttcctta tttacaataa
     9361 tagaattaat agatgaaaac ttaaagagca aatttaagct aggatatata ctacattgct
     9421 cttttatgat agaaagatgt tttaaaaata caacttttcc atataataac attactgaat
     9481 taattagtaa aaatcatgaa ctatatagtt taatacgtga agcctttaag ccaacagaag
     9541 cttattttaa tattttgata ccagacgaag aaattgctta tataatagac atgattaatg
     9601 agtatctaaa taaataaaat ttatagcact tattttgtgc atattaatta atatatgaaa
     9661 tggaggacga gattttgaaa ggaaaaataa aacagttaac aatacttgca ttaattttta
     9721 tatttataac tcctgtattt gcatttgcag atactccacc agtaccaaat tcttcaagag
     9781 cagcactact tatagaccag gaaactaaaa gaatattatt tgaaaaaaac attgatgaaa
     9841 aaatgccatt agcgagttta agtaaaatga tgaccttttt attggcaata gaagcagttg
     9901 ataaaaatca agttaaagag actgatatgg tgaaaataga caagtctaca gcttcagtag
     9961 gaggttctac ttgtaaactt aaagatggtg atgaaatatc tctaggagag cttatgcagg
    10021 gtctaatgct agtatctgga aatgatgctg ctatagccat agctaaacat attggtaaaa
    10081 ctgaaaagaa ttttgtaaat atgatgaaca agaaagcaga agaaatagga atgattgata
    10141 cgtattattt taatccaaat gggcttccta tatatactga cccagaacat aaagaacctc
    10201 ctattgaaaa tatgtcaact gctcacgata tagttacact tggaaagtat atgtacgacc
    10261 attatgaaaa tcaagttaca agaataacca ctatgcaagt atataatgat actaaaaagg
    10321 attttacaca ttataacaca aatcctctgc ttgtttcagt acctggtgtt gatggaataa
    10381 agacaggata tacagataat gctggatatt gccttgcatt ttctatgatg gtacctaaag
    10441 atgctaaaaa cgaaagaaat catagattga taggtgttgt acttggtgat ggtaacaaga
    10501 aaaatagaat ttcttcttcc gctactttat tgaaatatgg taaagataat ttccattcta
    10561 aaaagatagc acataagggt gatattatag agactccttg tgttgatggt attgatgatt
    10621 ttaagataac agttaaggtt gataaagatt tgtatggtgt tgtttcagat aacgagaata
    10681 tcaatcctaa ggtagtgttt aaaaatatga attatcctat ccataaggga gatattgttg
    10741 gtgttgcaaa gtattataat gactctggta aatttgttgg aagtgttgat gtaaaaagtg
    10801 aaagtaatat aggatgtatt cctttgaagg ataagattaa gattaaggtt gctaagataa
    10861 ataaaaaact tgagattaaa aattctgttt gttttaaagc atagtaaagc atattattaa
    10921 aagggtatgt taaatttata aagactccac tttgtggagt ctttttgttt tttacaaaaa
    10981 attaaaatag tggcaacaat tgaggatgtg agggagagtt gccactattt tgtatagggt
    11041 attatattat gtttttaagc tagttttact atcttctatg tttatataat aactcttgtg
    11101 tgtgaattta attgataatt catatgaaaa ttatgtgaag ttttgattta tttgtaatat
    11161 attctactac tattataatt tttctactgg atatactaat catttgttta acattatctg
    11221 agattgattt gcaaactttt atataactaa attagttata taacttatag attccattaa
    11281 ataccctaat aatcatatat gtacatacaa agtcatgctt atgcaaaatc tgcagtttca
    11341 actaacttat tatataaacc atctttatgt agaagttcag catcatttcc agattctatt
    11401 actctacctt tatctaatac atatatatta tcagcactct taattgttga aagtctatga
    11461 gctattataa taactatctt atcttgtatg tactctttca agttgttcaa tatctctttt
    11521 tgtgaaatat tatctagagc tgaagttgct tcgtccagaa taagtacctt tttattactt
    11581 actaaagctc tagctattga tatacgttgt ttttgacctc ctgatatttt gacacctttt
    11641 tctcctacta cttcgtccaa tcccttatct agatttttta taaaatcatc taaacaagta
    11701 acttctaata tctcatctaa ttctttatca cttattttat tatttaatac tatattattt
    11761 tttattgtgt catcaaatac aaattgatct tgagttacta tatcaatatt atttcttagt
    11821 gattttaaat taatatcctt tatattaaca ccatctatta atatatcacc ttcatttata
    11881 tcccaaagtc ggtatattag atttgttatt gttgatttgc cacatccact tccaccaaca
    11941 attcctgtta cttccccttt ctcaaattgt aaatttatat catttaaaac aaggtttaaa
    12001 tcctcaccat atctaaaaga tacattttta aaatctattt tcttaatttc actgttatct
    12061 attctatatc cattattatc aactttaata tctatctttt catctattac tgaaaatact
    12121 ctattgatag acacttttga ttgtgctatt ctagtattcg cccttactat agctatacat
    12181 ggtccaatta acattcctgt atattgttga aatgctatta actcccccat cgtcatctta
    12241 ttatttatta ttttatatcc cccaattcca tatattaata ttgttatcaa tcctgttagt
    12301 atttgagata ttgctatatt actactcata atgatgttca aattcaaatc tttttttatc
    12361 aaatctctct ctctctttaa atacttatta aagaaattct tttttgaatt taatataaca
    12421 atatccataa tattagatac gtactcttga atcaaatttg ataaactgcc tgaatcactt
    12481 cttatttcag aaattttctt agatataagt tttgtcattt tattttgaga aaatgttaat
    12541 atcaactgta aaaataaaac tactaaaaat aaatcaaact gcaaattaat taagaaaatt
    12601 aacgatatta tagctgtaaa cacatttaca ataatatcaa atataatctc tgctccaaag
    12661 ttttctatca taaatatatc actttccata atggataaaa tattacctgt tttcttactt
    12721 gaataatatc taccagaaag ttttgatata tggttaagca actttacttt tagatttata
    12781 gtaactttat tttttattga agtatataaa tatgataata taacatcaaa tgtagctgat
    12841 aatatcctta taaaaatata tatcaatatt aaatttataa gcagaaaaat atttttattt
    12901 cctattccat catctattat acatctaatt agttgaggta ttacaatatt tacgccagca
    12961 attataaaaa ccattataaa tgatactatc aacttagtct tattcttata tactattata
    13021 tttagtattc taaataaatt attattaata gtttttttca tctatttttc catcttttat
    13081 agttataatt ctatcagctg tctttgcaat attatcatta tgagttatca taactagtgt
    13141 ttgtttgtac tctttaacac accattcaag catattcata acttcatttg tagtcttact
    13201 atctaagttg ccagtaggtt catctgcaaa tattactgac ggtctattgg ctaaagctct
    13261 agctattgca actctttgtt gttgacctcc tgatagttca ttcggatatt tctttatttg
    13321 tttttcaatc cccaacttat taattaatct cataacatat tctttatctt cttttttccc
    13381 atcaagcaac acaggaagta ctatattatc atatacattt ataactggta ttaaattgta
    13441 actttgaaat atgaagccaa attcctgtct tcttatttca gacagcttat catcatttaa
    13501 tgtatataaa tctacatcat ctaagtacac attccctaaa gttggcttgt ctaagcctgc
    13561 aacacagtga agtagtgtac ttttaccaga accacttgca cctacaatag ctgtaaattt
    13621 atgtggctct atatctaaat caataccatc aagagctcta acttcattct catattttcc
    13681 atatattttt tttaaatctt ttaccttaag tattttcata acgtatctcc attcccattt
    13741 aaaaaacata tatttttaaa ttaaattttc gtaaataaga tctactgcca tatctaaatt
    13801 ctctaattga tataagtata cttctttgta gtcaatacta atattaaact ctttatttaa
    13861 aatagttaaa aaattatatg cattataatt tccaatacat gtaaaattgt taaataaaga
    13921 atattcactt acaaattcaa agtctatttt tattttagaa aagtttctat ttatcacatt
    13981 tattattttc cttgctatat ccaatcttat caaattagat ttataacttg gagtgttcaa
    14041 gttttctatt tttaaatctt ctttactttt gcttttttga ataagtattt cttccaattc
    14101 attaaactta atatatggat tgttttttaa tatatttgca acaatgccag ttgccactgc
    14161 acaagcctta ctatttttac caaaatgagt tactttatct ttaaatttaa taaaattatc
    14221 ttttgaattt gcatacattt gaatcttatt ttctggttcg tatatatatt gattattttt
    14281 atatatattt tcattaccta ctacaccaat aacactttca aatatagtag gaatactttc
    14341 tttatcttca tatttattat gttttgaaca aataataaat ctaccttgac tatttaaaat
    14401 attacaaacc ttttctaatt ctttttcata catactatta atagatgcag ctgaaatgtt
    14461 tattatctgt atatccgaca tagtcaactt gctcagtgta ctaagaagat ttaaagaact
    14521 tgtttttcca gaatcattaa atatattaat aggataaatt tgggtatttt tacttatatt
    14581 taatatggtt ttcgatacaa aagttccatg tccatttatg tcatttattt ccttcccccc
    14641 caaatctttc aattgaaaat ttgaatcata aacataattt ccattaatat catcatcatt
    14701 tacatctaat cctgtgtcta agactgctat tcttacttta gtcaattaat aatcacctct
    14761 aaaataaatt accttgaata taaaactaat ccttaccata atataggttc caagatgttt
    14821 ttcatttttt tacaatttgc atcaaacatt tttttgttat tttttaaagt atatattttc
    14881 gatggacaag ttgtacaaaa taaatttact ttacattctt tacaactctc aatatttact
    14941 ggttttaatt tatcaaatat attaaatata tccatttctc tattaagaat cctctctaat
    15001 atgttttcat taaattctaa tatattgaat atctttaaat ccttatactc taagtttgga
    15061 cacggatata catctccttt atgatctata cttatctgat taacacctgc tttacacata
    15121 tttgaacata aactactttc atccacataa tcatctttgc aaatgtaatc aatatcaagt
    15181 ttatcattaa gatatttgct atagttctca ttacctctac caatattcat aaaatttctt
    15241 attataggcg ttgtacctaa atcttcattt aatttattga atttctctat gtctttctca
    15301 ttattctttc ctaccaccat agacaaacct acgttctcga atttttttag ttttataagt
    15361 ctaatcacat ttaatacttt acagaatact cctttacctc ttacttcact acaacttaat
    15421 tcatcataac catctaaact aatttctatt gcatataaac cattaattat atcatcaatc
    15481 tgtttttctt taattaaagt tgcatttgta gacagaatta ttttaccttt aaatttttct
    15541 tttatataat ccaacagctc taagaaatca tttctcatca atggctctcc acctgtaatg
    15601 gttaatatcc ttggatttaa tttaactata ttatctatta cttttcttac ttgagatgta
    15661 tctaaataat cgttattatc tattttagaa tctacacagc aataatcaca acttagatta
    15721 catctattag ttggagaaaa tgaaattttg tttattctct ctagtctaaa ttcacttaga
    15781 atagaatcac ttagaagtcc tatatgctct aacttatcta taactcgttc ataatatttt
    15841 ttatcttcat tttcttggaa tacatcatac aaattaccaa gttgtaatgc attatctact
    15901 gaatacttta gaacttcata acattctatt ggaattttaa cccatcttcc atttacttta
    15961 ttactaagaa taacttgatt attatttatt attttttcac atatacaatc taaaaaatat
    16021 ttgtttgagc tattcattta ttttatttcc ttcctatata tttaaattag aaatttcatt
    16081 aattttcttt tataccactt actatactaa gtttatttat cattttatta gaaaaataaa
    16141 ctgcaatgac acaaacaaaa acagatatag atccaaatat tagaatttca tatattggaa
    16201 tttcaaattt aatagagtta tttataccat atacctgttg attaagtttg ttagtttcag
    16261 aaactatttt ataataattg aaactagata taattccagc tacaatcata cttaataccc
    16321 catatatcat attttctttt attaacattc tttttacatt ttttttggac atccctattg
    16381 ctctaaatgt acatatctca ctcaacctca caattatatt tgatctaact ataaaaataa
    16441 tgtttataga tgatattaat aaaactccaa atacacaaat cattgctaat ttatatgagg
    16501 atttattata atggtctcca aaatgattac gtacatatct tccctctata tctgaaaatc
    16561 cacttttttc tattatatta tttatttctt ttattacttt tttatctgtt tcttttttta
    16621 tcttcacaaa aactttatta taatcactta tccctgttaa ttcttttaaa tccttttcat
    16681 taaatataat ttgaattcca cctgattgag attctccatc ttgctctgat atatagctat
    16741 ttttcattat tccagcgatt cttacttttg tatttatata tttatactct ccatctttat
    16801 atacaggaat ttttatatct attaagtctc ctattttagg tgattttata atctgagtat
    16861 tatttgacaa tttaattcta gaataaaaat tatttaccaa taaaacattt ttataatccc
    16921 tgtctgtata actataaaca ttttcgcctt tatctatata tttatctata ttatttaata
    16981 gctctttatt gtaacctcga attaaaactg tagactcatt attattattt atttcagttc
    17041 tttttagctc atctaaataa tcttcttcta agtcagcatt tcttagatat ccttttaaat
    17101 aaatattggg ttcaacatct gttacatctt caatagcttt tatttcattt aataaagaat
    17161 tatcatattt agaaaatgta taatttgcat ttattggtga atatcccaaa taaatatcaa
    17221 aattgccata tgatttactt gttatattag ctgaatctga ctcttttcca tatatgaatt
    17281 ttgtagaagt tagtctgcct ataaataaaa ccccacatag tgatatcgta attattatta
    17341 ttattgatgt aaccttattc ctaaatatat ttttaaaagc tatctcattt ataattccaa
    17401 caactttttt atgtgatttt aacaacaaaa gcctaggaat ataatttaac ttatctgtct
    17461 tttttatagc atcaattata gataaatttc cagctttata tattggtact acagaggcta
    17521 atataactgc tataattgac atgatgatag attcaattat agagtttgta tttatagtta
    17581 attctgcact tttatcatat actatcatga gacctaagta agaaaaaact atacctccaa
    17641 gtaaaccaat tactatacct acagataaaa ctacaatact ctgaataaaa aatacctgtc
    17701 taatatttct cttgctagct ccaacagctc taagtaaacc aatttgcttg gtcatatcta
    17761 gtattattat gttaaacata ttgtatatta ctaatgttgc acataataaa gggagtaagc
    17821 tttgctttat tgaataagtt gttttttttg aaatattttt catatacata gacatactta
    17881 cttctgtatt tggataaaat tgattacttg ttaacttata ttttctccct agattcacta
    17941 ctttttgaga atctaaatct tttgtattca attttaatgt acccatatga gttattaatt
    18001 catatggaac aatatctttg ccattgtttg aatatgtaaa agctcttacc ttatatttat
    18061 ttaaattttc atagtattca tcagatttac ttaaaatacc aactatttta aattgcttat
    18121 ctttactata aatagaattt tcattatttt tatttatatg ctcattaaca atctgaaaat
    18181 caatagtttg atttaatttg acatctaatt ccattttgtc taaaactttt tgttctatta
    18241 ctaattcatt agaagttcta ggctctctac ctttaactaa cttatatcta gtagaatcta
    18301 aaaaatcttt attgtaagag cataaattta gagatgctcc attatcttta cttataattt
    18361 ctccaaattt aagtacatca ttgaattctt ttatatcttc ttcaccatta agttctttta
    18421 ctatatcact atttatatct tcataaaaag catgataatc tcccgagttt ttgtatgcat
    18481 gatttatatc tgattgtcct tgagattcat taataacact taatgaaaaa actagcataa
    18541 cagcaatccc tatactaagt attaatgata aagtttttcc tttttgtctc tttaaatatg
    18601 acaccgcaag tttaatattt atcataatca gaatcctcct tattcatatt tacaagaaaa
    18661 actctataac ctaaaatagt aggttataga gcactattca ttaaatacta actaactcgt
    18721 tgtgctagtt aaatttaatt attaaaaata ctagagttga gaaaatcaca tttcatttgt
    18781 aaaaaactac atctagtact ttcaacatat ttaatagact gattagtatt ttttaataag
    18841 ctactgttcc ttttggagtt gatcctgcac aatccaccat acagtctatt tgtgaaatta
    18901 ttccatgctt atcataatac catttacatg gatctgcttg cacctcaaca tcttgaagtt
    18961 ctattttttc ttcgattttt tttatatgta tcataataca tatccccctt tacttttgtt
    19021 ttatatttat tataactagt taaactagat tataaactac atagtttttt tgtcgtttcc
    19081 acataaactc tttctaatcc acaataaatt ggtactgcac tttggaattt acctgttacc
    19141 tttttattga taagtttaca tctggttgac atacaataat caagtctagt gcatccatca
    19201 cactcttcat tcttagatat ataaattttt ataaggttct taattttttt atcatctaat
    19261 ccatcatata tatttcctag taaaatatct ttatctccta cacatactgt acatggatat
    19321 acatctcctc tagtatctat tgcataacta tttatccctc caaaacaatc tccttttttc
    19381 ttatttataa attgtttatc taaaatagat atatctatct caggataatc ctttttaata
    19441 ttcataattt tactaatttg ttcatttaat attttaatat gagaattatc ccaattttta
    19501 tccataaaat ctgaaattgg tgcgatattt ttaaaccctt gttttgctat atcatataca
    19561 ctctcataaa gatacttaac agtatttgag ttatatgtca ttcttgccct aacatatata
    19621 cctttatcta taagtctttt tccattttcc attacttttt cataagtacc tactccgtta
    19681 gcatctattc tatttatatc atgtatattc ttttttccat caatacttat cgatatgttt
    19741 tttacattct ttgctataaa ctctattatt tcatcattta taatagttcc atttgtagtt
    19801 atatcaaata atatcttttt gtcactcaat ctatctacta aatccctatg tatatattta
    19861 atcaaatcaa aatttataaa tggttctcct ccatgtaaaa caattgttgt ataatcacta
    19921 ttactttcta attctttatt acaaataaaa tctattgttt tatcagcagt ttcataactc
    19981 atattttctt tttttttatc attaccttca taacaatatg tacatctcaa attgcagtct
    20041 tcagttacaa atatagtaaa tttcaattaa ctttttcatc tccccataca gctgtattta
    20101 aattgtgttt catcatacta caccatctat ccaattccaa ttcattatcc ttaactgaat
    20161 ctaatatcgc tgggcaatgc caacaaaaaa tatttacatc acaatcttta catttttcaa
    20221 agttatatgg ataaatactt tctaatttaa tcaaagactt tagatctctt gttttatttt
    20281 cttttataac ttttacaaat tcattgtcaa atatatttcc cattttatag tcatccttta
    20341 tcagtgaagg acaaggatat acatctccat cataatttat aaatatttga gagtttcctg
    20401 cattacatgc acaacatccc atttttttat tttttttatc tttacacatt tttaattcat
    20461 tatctaaaat atacggaatt gtaattggaa gaactgtatc ttcaattaaa tagctttctt
    20521 tattttctag gcctcttcct gttggaataa aatatctttc aacaaattct gttccaagct
    20581 ttttatttaa atctttaaat tgtttattta aatgagaatt tttttgtcca aataccatag
    20641 acagctttat attactgaat ccattgtctt ttagcagatt aacagagctt attactttat
    20701 taaatacacc ttttcctcgt gtttcactac aagtattttc atctactcca tcaatactaa
    20761 tatctatttt ttcaaaatta gttataataa aatcaacatt atgttcatct attaaagttc
    20821 catttgtgga taatattaat ttggtatcag gtaatttttg ttttatatat ttaattattt
    20881 caaaaaaatc ttctcttatc aatggttctc caccagttat aattatatga ggagtattta
    20941 aaactgatat tatatctacg atatttttta aatcatatgt actaagtaaa tctttcttat
    21001 tagaagaaga ttcaacacaa caatgtttac aaaataaatt gcatctattt gtcaaacaaa
    21061 aataaattgc aacaccattg cttaacatat caaagtttaa attgttactt ttaaaaattt
    21121 gatttttgtt cataatatct ataagattaa taatatattt tttgtcactt tcatcatcaa
    21181 aataatttac aaaatcataa atttttattt tattttct
//
LOCUS       DAELNY010000056        20764 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.056, whole genome shotgun sequence.
ACCESSION   DAELNY010000056 DAELNY010000000
VERSION     DAELNY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.056"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="KPE55_001735"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="KPE55_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG0492821.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="KPE55_001736"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="KPE55_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG0492822.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="KPE55_001737"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="KPE55_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG0492823.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="KPE55_001738"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="KPE55_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG0492824.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="KPE55_001739"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="KPE55_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG0492825.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="KPE55_001740"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="KPE55_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG0492826.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="KPE55_001741"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="KPE55_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG0492827.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4711
                     /gene="rpsC"
                     /locus_tag="KPE55_001742"
     CDS             3911..4711
                     /gene="rpsC"
                     /locus_tag="KPE55_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG0492828.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4747..5178
                     /gene="rplP"
                     /locus_tag="KPE55_001743"
     CDS             4747..5178
                     /gene="rplP"
                     /locus_tag="KPE55_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG0492829.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5180..5383
                     /gene="rpmC"
                     /locus_tag="KPE55_001744"
     CDS             5180..5383
                     /gene="rpmC"
                     /locus_tag="KPE55_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG0492830.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5407..5661
                     /gene="rpsQ"
                     /locus_tag="KPE55_001745"
     CDS             5407..5661
                     /gene="rpsQ"
                     /locus_tag="KPE55_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG0492831.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5687..6055
                     /gene="rplN"
                     /locus_tag="KPE55_001746"
     CDS             5687..6055
                     /gene="rplN"
                     /locus_tag="KPE55_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG0492832.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6079..6384
                     /gene="rplX"
                     /locus_tag="KPE55_001747"
     CDS             6079..6384
                     /gene="rplX"
                     /locus_tag="KPE55_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG0492833.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6414..6956
                     /gene="rplE"
                     /locus_tag="KPE55_001748"
     CDS             6414..6956
                     /gene="rplE"
                     /locus_tag="KPE55_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG0492834.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6974..7159
                     /locus_tag="KPE55_001749"
     CDS             6974..7159
                     /locus_tag="KPE55_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG0492835.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7192..7590
                     /gene="rpsH"
                     /locus_tag="KPE55_001750"
     CDS             7192..7590
                     /gene="rpsH"
                     /locus_tag="KPE55_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG0492836.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7621..8163
                     /gene="rplF"
                     /locus_tag="KPE55_001751"
     CDS             7621..8163
                     /gene="rplF"
                     /locus_tag="KPE55_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG0492837.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8199..8567
                     /gene="rplR"
                     /locus_tag="KPE55_001752"
     CDS             8199..8567
                     /gene="rplR"
                     /locus_tag="KPE55_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG0492838.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8588..9097
                     /gene="rpsE"
                     /locus_tag="KPE55_001753"
     CDS             8588..9097
                     /gene="rpsE"
                     /locus_tag="KPE55_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG0492839.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9112..9297
                     /gene="rpmD"
                     /locus_tag="KPE55_001754"
     CDS             9112..9297
                     /gene="rpmD"
                     /locus_tag="KPE55_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG0492840.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9330..9773
                     /gene="rplO"
                     /locus_tag="KPE55_001755"
     CDS             9330..9773
                     /gene="rplO"
                     /locus_tag="KPE55_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG0492841.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9817..11085
                     /gene="secY"
                     /locus_tag="KPE55_001756"
     CDS             9817..11085
                     /gene="secY"
                     /locus_tag="KPE55_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG0492842.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11102..11752
                     /locus_tag="KPE55_001757"
     CDS             11102..11752
                     /locus_tag="KPE55_001757"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG0492843.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11753..12499
                     /gene="map"
                     /locus_tag="KPE55_001758"
     CDS             11753..12499
                     /gene="map"
                     /locus_tag="KPE55_001758"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG0492844.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12521..12790
                     /locus_tag="KPE55_001759"
     CDS             12521..12790
                     /locus_tag="KPE55_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG0492845.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12828..13046
                     /gene="infA"
                     /locus_tag="KPE55_001760"
     CDS             12828..13046
                     /gene="infA"
                     /locus_tag="KPE55_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG0492846.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13082..13195
                     /gene="rpmJ"
                     /locus_tag="KPE55_001761"
     CDS             13082..13195
                     /gene="rpmJ"
                     /locus_tag="KPE55_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG0492847.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13348..13719
                     /gene="rpsM"
                     /locus_tag="KPE55_001762"
     CDS             13348..13719
                     /gene="rpsM"
                     /locus_tag="KPE55_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG0492848.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13755..14153
                     /gene="rpsK"
                     /locus_tag="KPE55_001763"
     CDS             13755..14153
                     /gene="rpsK"
                     /locus_tag="KPE55_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG0492849.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14185..14808
                     /gene="rpsD"
                     /locus_tag="KPE55_001764"
     CDS             14185..14808
                     /gene="rpsD"
                     /locus_tag="KPE55_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG0492850.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14886..15833
                     /locus_tag="KPE55_001765"
     CDS             14886..15833
                     /locus_tag="KPE55_001765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG0492851.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15854..16195
                     /gene="rplQ"
                     /locus_tag="KPE55_001766"
     CDS             15854..16195
                     /gene="rplQ"
                     /locus_tag="KPE55_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG0492852.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16315..17148
                     /locus_tag="KPE55_001767"
     CDS             16315..17148
                     /locus_tag="KPE55_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0492853.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17136..18002
                     /locus_tag="KPE55_001768"
     CDS             17136..18002
                     /locus_tag="KPE55_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0492854.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17996..18799
                     /locus_tag="KPE55_001769"
     CDS             17996..18799
                     /locus_tag="KPE55_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG0492855.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18831..19562
                     /gene="truA"
                     /locus_tag="KPE55_001770"
     CDS             18831..19562
                     /gene="truA"
                     /locus_tag="KPE55_001770"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0492856.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19682..20113
                     /gene="rplM"
                     /locus_tag="KPE55_001771"
     CDS             19682..20113
                     /gene="rplM"
                     /locus_tag="KPE55_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG0492857.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVIVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20142..20534
                     /gene="rpsI"
                     /locus_tag="KPE55_001772"
     CDS             20142..20534
                     /gene="rpsI"
                     /locus_tag="KPE55_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG0492858.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8292 a         2450 c         4317 g         5705 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgaaaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgataaca gaggaaacta cagaggacaa agacctcaag gtggatcaag
     4681 acctcaaaga actgaaaaca aaggaaatta ataatagtta ggttcaagga aggaggaaaa
     4741 atactcatgt taatgccaaa aagagtaaag cgtcgtagag ttcatagagg aagcatggct
     4801 gggcaagctc ataagggtaa taaagttact tatggtgagt ttggattagt agcattagaa
     4861 gcttcttgga taacttctaa tcaaatagaa gctgccagaa ttgcgatgac tagatatata
     4921 aaaagagggg gaaaggtttg gataaaaata ttccctcata aaccagtaac aagaaaacca
     4981 gcagagactc gtatgggtgc agggaaaggt tctccagaat attgggtagc tgtagttaag
     5041 ccaggaagag ttatgtttga attagcaggt gtttctgaag ataaagcaag agaagctatg
     5101 agacttgctg cacataaatt accaatcaaa tgtaaatttg ttaaaaaaga agatttagaa
     5161 gtaaagggtg gtgaatagga tgaaagctaa agaattaaga gatttaacaa gcgaagagct
     5221 aatgaacaaa ttaaacgact tcaaaagtga attatttagc ttaagattcc aattagctac
     5281 tggtcaatta gaaaatacag ctagaataaa gtttgttaag aaggatatag caaaagttaa
     5341 aactgttctt gctgaaagaa agttatacga aactagagct taatttggaa aggaggcttt
     5401 ttaaacatgg aaagaggaag aagaaaagtt agaataggcc gtgttgttag tgataagatg
     5461 gataaaacaa tagtagtagc tgttgaagaa ttcgtacgtc atccactata taataaacgt
     5521 gttaagagaa ctaagaaatt caaggctcat gatgaaaaga atatatgtaa catcggagat
     5581 agagttaaaa taatggaaac tagaccatta tctaaagata agagattcag actagttgaa
     5641 gttgttgaga aagttaagta gtttttgaag aaggagggat tacgatatga tacaacaaga
     5701 atcacgtcta agagttgctg ataactcagg agctaaagaa cttttatgta tacgtgttct
     5761 aggcggaagt aaaagaagat atggtaacat aggcgacgtt atagttgcta ctgttaaaag
     5821 tgcaacacct ggtggagttg taaaaaaagg taaggttgtt aaagctgtta tagtaagaag
     5881 caaacaaggc gtaagacgta atgacggaag ctatatatct tttgatgaaa atgctgctgt
     5941 tataataaag gacgataaaa ctccagtagg aactcgtata ttcgggcctg ttgctagaga
     6001 gttaagagac aatgaattca tgaaaatagt ttctcttgct ccagaagtac tataattaag
     6061 gaggtgcaat aggacatgat gcgtgttaaa aaaggtgaca ctgttgtagt tatagcaggt
     6121 aaagataaag gtaaaaaagg ttcagtttta aaggtatatc ctaaaactag caaagtgtta
     6181 gttgaaggtg taaatgtaat aactaaacat caaaaaccaa gtgctatgaa ccaacaaggt
     6241 ggaataataa ataaagaagc cccaatacac atatctaatg taatgccatt tgatcctgaa
     6301 acaggaaaag gcgttagagt aagatatgaa gtaaaagatg ggaacaaagt aagagtatca
     6361 gcaaagagcg gaaaagaatt ataataaata ttgcttgaaa ggagggacct taaatggctt
     6421 ctagattaca agaaaaatac atgaaagaag ttgctcctgc tttaatggag aaatttggat
     6481 acaaaaacgt aatggagata cctaagttaa ataaaatagt tattaacatg ggtataggtg
     6541 acgctagaga aaatccaaaa ggattagaaa aaggcgttga agaattagaa atgatatcag
     6601 gacaaaagcc tgttataact aaagctagaa aatctgttgc taacttcaaa ttaagagaag
     6661 gaatgccaat aggaacaaaa gttacattaa gagctgacaa aatgttctac tttatggaca
     6721 aattagtttc agtttcttta ccaagagtta gagactttag aggagttaac cctaatgctt
     6781 ttgatggtag aggaaattat gctttaggag ttaaagaaca attaatattc cctgaaatag
     6841 aatatgataa aatagataaa gtaagaggaa tggatataat atttgttact acagcaaaaa
     6901 ctgacgaaga agctcgtgaa ttattaaaat tattaggaat gccattttct aagtaagcaa
     6961 aggagggatt cccgtggcta gaaaagcgat ggttgttaag caacaaagaa agcaaaagta
     7021 cgctactaga gaatacacta gatgtactat atgcggaaga ccgcattcag ttttgaaaaa
     7081 atttggtata tgccgtatat gctttagaga attagcttat aaaggtcaaa tacctggtgt
     7141 tagaaaagca agctggtaga agcttgatta gaatggaagg agggcaacaa aatgacaatg
     7201 acagatccaa ttgcagatat gcttactcgt ataagaaatg ctaatgttgt taagcatgaa
     7261 actgtagatg ttccagcttc taatatgaag aaagaattag ctagaatctt attagaagaa
     7321 ggtttcataa gaggttatga tgttatagaa gatggaaaac aaggtatcat aagaatacaa
     7381 ttaaagtacg gacaagaagg cgaaagagtt ataacaggtc ttaagaaaat atctaagcct
     7441 ggaatgagag tttacgctgc taaccatgag ataccaaaag tattaaacgg attaggaata
     7501 tcagttatat ctacttcaaa aggaatatta actgacaagc aagctagaaa agaaaatgtt
     7561 ggtggagaag taatctgcta cgtttggtaa ttaatatgaa caatgtaagg aggtgcgacc
     7621 atgtcaagaa taggtgttaa gccaataata ataccagcag gtgttgaagt tactatagct
     7681 gaaggaaatt tagttacagt aaaaggtcca aaaggaactt taactaaaca attaagtgct
     7741 gagttaaaca taaaaaaaga agaaaataca ataatggttg aaagaccaac tgataataaa
     7801 aaacatagat ctttacacgg actaactaga actttattag ataatatggt tgtaggtgta
     7861 aatactggtt tcgaaaagaa attagaatta aaaggtgttg gatatagagc tcaaaaacaa
     7921 ggaaagaaat tagttatgaa cttaggtttc tctcatccag ttgagatgga agatccagaa
     7981 ggaataactg ttgaagctcc aaaccaaaca gagttaatag taaaaggtat agacaagcaa
     8041 ttagtaggga attatgctgc taagataaga gcttggagaa agccagagcc atacaaaggt
     8101 aaaggtataa aatacgttga tgaagtaatc agacgtaaag aaggaaaaac tggtaaaaaa
     8161 taataccaaa acttaaacgc tagaaaggag tgagctaggt gttaaaaaag gctgataaaa
     8221 atgctaatag acttcaaaga cataagagag ttcgtagaaa aataagcggt acttctcaaa
     8281 gaccaagatt atgtgtattt agaagtgcta acaatatata tgctcaaata atagatgaca
     8341 caaaaagagt aactctagtt gctgcatctt ctttagaagc agaagttaag agtgctgtca
     8401 atcatacagg aaacaaggaa gcagctaaga aagttggaga attagttgct aaaaaagctg
     8461 ttgaaaaagg aatcactgaa gttgtatttg acagaggtgg atacttatat catggaagaa
     8521 ttcaagaatt agctgaaggt gctagagaag ctggtcttaa gttctaatac gaaggaggga
     8581 aaaatacatg ctacgtcgta agccgataga tgcaggacaa cttgacttac aagaaaaagt
     8641 tgttgaagta agacgtgtta ctaaggttgt aaaaggtggt agaaatttca gatttgcagc
     8701 attagtagtt gttggagatg aaaacggaca cgttggtata ggtgctggga aagcaatgga
     8761 agtaccagac gctataaaaa aagcagttga agatgctaag aaaaatctaa tagtagtgcc
     8821 aatagttggg actacaattc ctcacgaagt acgcggacac tttggtgctg gaaatatatt
     8881 aataatgcct gctgtagaag gtacaggagt aatagctggg ggacctgcta gagccgtact
     8941 tgagctagct ggactaaaag acgttagagc taaatcttta gggtctaaca atccaagaaa
     9001 tatggtaaat gctacaatag aaggacttaa ttctctaaaa acagttgaag atatagctaa
     9061 acttagaggt aaaaaagttg aagaacttct agggtaagga ggtagtgtaa aatggctaaa
     9121 ttacaaataa agttagttag aagtgttata ggaactactc ctaaccaaaa aaagaatgtt
     9181 gaagcgttag gattaagaaa aagagaacaa gttgttgtaa aagaagacaa tgctcaaaca
     9241 agaggtatga taaataaagt aagtcactta ttagaagtaa ctgaaatagc tgaataattt
     9301 ttataaagta gtgcaaggag gtgcaatcca tgaagttaca tgagttaaaa cctgctgaag
     9361 gtgcagtaag agctaagaga agattaggta gaggtactgc aactggacaa ggtaaaactg
     9421 caggacgtgg acaaaaaggt caatggtctc gttctggtgg tggagtaaga gtaggatttg
     9481 aaggtggaca aatgccacta gctagaagac ttcctaagag aggtttcaat aacatattta
     9541 agaaagttta cacagaagtt aatgttgaag ttttaaacag atttgaaaat ggaacagaaa
     9601 taactgcaga attattaaaa tctactaaaa ctataagcaa aataggtaaa gacggaatta
     9661 aaatcttagg tgaaggtaat ttagagaaag ctttaactgt taaagctgct aaatttacag
     9721 cttcagctca agaaaaaata gaaaaagctg gtggaaaagc agagttagta taataacctg
     9781 ctaatctcct agccagtaca ggcggggtga attagcgtgc tgtcaaaatt aaaacaagct
     9841 tggaagatta aagcagtaag aaaaaaagta atgtatacac ttatgatgat cgtgatattc
     9901 agaataggta ctactatacc agttccaggc atagacacta gtattataca aaagatggta
     9961 ggcggcaata gtctactttc tctgtataat atgtttactg gtggagcatt tagtaacttt
    10021 tcactatttg ctttaggtat aagtccatat atcactgcat caatcataat acaacttcta
    10081 acagtaggtt ttgaaagttt agcagaactt caaaaatctg gtgaagaagg aaagaaaaaa
    10141 atcaataagt acactaaata tacagcacta gctctagcag ttgtacaagc tttgggaatt
    10201 acattaggaa ttgtaagaag tgctttaata tcaaatagtg tgttttttat cacaactgtt
    10261 gtaataacat tagtttcagc aagtatgtta gtaatgtgga ttggggacaa aattactgaa
    10321 aaaggaatcg gtaatggtag ttcagtaata atatttgcag gtataatatc aagaatacct
    10381 acagatgtta ttaaaatatc gcaacaagtt aaatctggcg aagttgcacc ttgggttata
    10441 gtgattctag ctgtagttat attacttaca gtaacaggag ttacatttat acaagaagca
    10501 actagaaaga tacctgtaca atatgctaaa agagttgttg gaagaaaaat gtatggagga
    10561 caaagttctc atataccaat gaaagttaac cagtcaggag ttatgcctat aatttttgct
    10621 agttcacttt tagctttccc tcaaacaata gctatgttta tgggtccaaa tgctcaggct
    10681 tttgttcaaa aatacttaag tatggcaaca gaacaaggat tttggacata tagatcaatt
    10741 gagattctac ttataatttt cttctcatat ttttatacaa ctgtatcatt taatacagaa
    10801 gacatatcta aaaatatgaa aaataatggt ggatttatac caggtataag accaggtgag
    10861 cctactatgg attatttaaa tagaatttta actagattaa ctttagctgg agctactttc
    10921 ttagcaataa ttgctatggt gccagctctt acaacacatt atatgaaagt aaatatgagt
    10981 ctagctggga cttcattgct aatcgttgtt ggagttgctt tggagcttaa gagacagtta
    11041 gagtcaaact tagttatgag aagctatcaa ggtttcttaa aataaaatgg agatggataa
    11101 tatgagaata atattacttg gacctccagg tgctggtaaa ggtactcagg cggcaggaat
    11161 agtagaaaaa tacaatatac ctcatatatc aacaggagat atattcagaa agaatataaa
    11221 agagggaaca gaacttggaa aaaaagctaa agaatacatg gaccaaggtt tattagtacc
    11281 agatgagtta actgtaggtt tagttactga tagaatatct caagaagatt gtaaaaatgg
    11341 atttatgtta gatggatttc caagaaatgt agcacaagga gaacatttag atatcttctt
    11401 aaaaaatgct ggtatatcac tagataaagt tgtcaatatt gaagttgata agagtatatt
    11461 agtgtctaga gcagttggta gaagaatatg taagtcttgt ggagctactt accatgttga
    11521 gtttaatcct cctaaagtag aaggtgtatg tgatgtatgc caaggagaat tatatcaaag
    11581 agctgatgat aatgaagaaa ctgtatctaa gagaatacaa gtttatctag atgaaactaa
    11641 gcctttagta gattattata gcaaacaagg tataatagca gatataaaag gtgatcaagc
    11701 aatagataaa gtatttgaag atattgtcgc agccttagga agtggaaaat aaatgattat
    11761 cttgaaatca aaaaaagaaa tagaactttt aagagaagca ggtaaaatag ttgctgacac
    11821 tcatgaagtt ttaagaaaag ctatttctcc gggaatatct actttagaat tagataaaat
    11881 agctgaagag aatataagaa aatacaatgc agaaccttct tttaaaggat atggaggttt
    11941 tccaggaagt atatgtgctt ctatcaatag agaggtagta catggtattc caggagaaac
    12001 tatattacaa gaaggtgaca ttgtaagttt ggatatagga gcctattata aaggatatca
    12061 tggtgattct gcgaaaactc acggagtagg catgatatct gaagaagata ggaaattgat
    12121 agaagtaact agagaaagct tctatgaagg aataaaattt gctaaattag gatatagact
    12181 ttcagatatc tcacacgcag tacaaacaca cgttgagaaa catggtttct cggtagttag
    12241 agatttagtg ggtcatggag ttggagcaaa tcttcatgaa gacccacaag tacctaatta
    12301 tggactccca ggaaaaggac caagacttag agaaggaatg gttattgcta tagagccaat
    12361 ggttaatgca ggtcgttatc atgtaaagac tctatcagat gggtggacga ctgttacaat
    12421 agatggaaaa aaatcagctc attatgagca tacgatagca attactgaac atgaaccttt
    12481 gatattgaca aagttgtaat ttaaaaagta ggtgaaaaaa gtgctatcag ataatttaag
    12541 tataggtcaa gttgttaaag tttcattagg acgagataaa ggaaatttat tctttgtagt
    12601 caaaataatt aataatgaat atgtattaat agctgatgga aaaaagagaa aacttgacaa
    12661 acctaagtta aagaaagtga agcacttaaa aaaatataat tttattaatg atgaagttag
    12721 aaaaagagta gtatctggac aagagattac agattctttt ttaagagctg aacttactaa
    12781 gttaaattag ataatttaac ttattgaaat ggagggctgg ttagttaatg gccaaaaaag
    12841 atgttataga attagaaggt acagtttcag aagctttacc taatgctatg ttcaaagtta
    12901 aactagaaaa tggacatgag atattatgcc acatttctgg aaagctaaga atgaacttca
    12961 taagaattct tgaaggtgac aaggtgaatg ttgaactttc tccatatgac cttacaagag
    13021 gaagaattac ttggcgtaag aagtagacta tattaatatt tagtctaagg agggattaat
    13081 aatgaaggtt agaccatcag taaaaccaat atgtgaaaaa tgtaaagtaa taaaaagaaa
    13141 aggaaaagta atggttatct gtgaaaatcc aaagcataag caaaagcaag gataagaaat
    13201 tattactagc attttgttaa attttatagt ataatactat attgtgatgt taacaaaaga
    13261 acgttaaaat cgttgaacaa tctggcgtag gattggcata tgatgtgaag ttttaaacac
    13321 taaaaactat gtgtaggagg tgcgatcatg gcaagaatag ctggggtaga tttacctaga
    13381 gaaaaaagag cggagatagg cttaacttat atatatggta taggtaaagc aactgctaac
    13441 gaaatattag ctaaggctga gataaatcca gatactagaa taaaagattt atcagaagat
    13501 caagttaatg agttaagaaa agttatagat gacgatttct tagttgaagg tgacttaaga
    13561 agagaaattg ctttaaacat aaagagatta agagatataa aatgttatag aggaataaga
    13621 cacgctaaag gtcttccatt aagaggacaa agaactaaga ctaatgctag aactagaaaa
    13681 ggtcctagaa aaacagtatc tcgtaaaaag aaaaaataaa taaataataa ctagatgaag
    13741 gagggaaaag gaaaatggct aaaccaaaaa agaaagttac acgtattaga agaagagaac
    13801 gtaaaaatat agaacgtggt catgctcata tacaatcaac tttcaataat acaataataa
    13861 ctttaactga cgttcacgga aatgctatat cttgggcaag ttctggacaa ttaggattca
    13921 aaggatcaag aaaatcaact ccatttgcat ctcaaatggc tgctgaaaca gctgcaaaag
    13981 ctgcgatgga acacggacta aaaagtgttg aggtattcgt aaaggggcca ggttcaggaa
    14041 gagaagctgc aataagagct ttacaagcaa ctggactaga agtaactatg ataaaagacg
    14101 ttactccaat cccacataac ggatgcagac caccaaagag aagaagagtg taattaataa
    14161 gagtacaata ggaggtgtag ataaatggca agatatacag gtgcatcatg tagacaatgc
    14221 cgtagagagg gaatgaaatt attccttaaa ggtgatagat gttatacaga caaatgtgct
    14281 atagtaaaaa gaaactatgc tccaggtcaa catggccaag gaagaaagaa agtttctaac
    14341 tatggattac aattaagaga aaaacaaaaa gttaaaagaa tatatggagt tttagagact
    14401 caatttagaa atttatatga acgtgctgaa aatatgcctg gtaaagcagg tgagaactta
    14461 ttaagtttat tagagagaag attagataat gtagtttaca gaatgggact agcatcttct
    14521 agaaaagaag ctagacaatt agtgactcat ggtcatttca ctttaaatgg aaataaagta
    14581 gacatacctt cattaatagt taaagttgga gatgttatag aagttaaaga gaaatcaaga
    14641 tcttctgcaa aattcaaaaa tttagtagaa gttaactcaa gaatagctcc taagtggtta
    14701 gaagctaatg tagaaggaat gacagcaaaa gtagttggtg ttccaacaag agaagatata
    14761 gatcttgaga tagcagagca cttaatcata gaactttact ctaaataata agcacacttt
    14821 taaaaattat tttaaaattt tgtttaccct cagtggatta ctgaatttta aggagggttt
    14881 tgtccatgat agaaatagaa aagccaaaag tagatatagt tgagcttagt gaagactata
    14941 gatatggtaa gtttgttata gaacctctag aaagaggata tggaataact ataggtaacg
    15001 cattaagaag aatattatta tcatcattac caggtgtagc agtgaatgct ataaaaatag
    15061 atggagttct tcatgaattt tcaacaatac ctggtgttaa agaagatgtt actgagataa
    15121 tattaacttt aaaagagctt tcagcaacta tagatggtga aggaagtaga acacttaaaa
    15181 tagaagctca aggaccatgc tctatcacag gtgcagatat aatctgtcct ccagatgttg
    15241 aaatattaag taaagattta gcaatagcta cattagatga taatgctaaa cttaatatgg
    15301 agatatttgt agataaaggt agaggttatg tttctgctga agaaaataaa acagagaatg
    15361 ttccaatagg tgttttacct gtggattcaa tatatactcc tgttgagaag gttagttatc
    15421 atgtggaaaa cacaagagtt ggtcaaaaaa cagattatga taaattagta ctagaagttt
    15481 ggactaatgg tagtataaat cctcaagagg gaatatcatt agctgcaaag gtattagttg
    15541 agcatttaaa cttatttata gacttaactg aacatgtaag tagtgttgaa atcatggtag
    15601 aaaaagaaga agatcaaaaa gaaaaagttc ttgaaatgac tatagaagaa ttagatttat
    15661 cagttagatc atataactgt ttaaagagag cgggaattaa tacagttgaa gaattagcta
    15721 ataaatctga ggatgatatg atgaaggtta gaaacctagg taagaagtca ttagaagagg
    15781 taatacaaaa actagaagaa cttggattgg gattaaaacc aagtgaagaa tagcgagcaa
    15841 aggagggata agaatggcta agtaccgtaa attaggacgt gaaacagctc acagaaacct
    15901 tatgttaaga aacttagtaa cttgcttact aagaagtgga agaatagaaa ctacagtgac
    15961 tagagcgaaa gaaactcgta gaatggctga aaagatgata actcttgcta agagaggaga
    16021 tcttcatgct agaagacaag ttttagctta tgttatggat gaaacagtag ttaataactt
    16081 attcacagat ttagctccaa aatatgctga gagaaatggt ggatacacta gaataataaa
    16141 aatagggcca agaaaaggcg atgctgctga aatggctttt atagaattag tatagtaagg
    16201 aaaggattag gcttgatggc ttaatctttt ttttattttt ttgactaagg tatgatataa
    16261 taatgtgtaa aatgattttt ataagttatt gcttactaaa aggagtatga gttaatggat
    16321 aatatagtaa aggtaaataa tatttcgttt gagtatatta cagatgaagc aaaacttaaa
    16381 gcaatagata atttaagcct agatgttaaa aaaggagaat ttgttgcgat aataggacat
    16441 aatggttctg gtaagtctac tttatctaaa aacttaaatg ctattcttat gcccactgaa
    16501 ggaaatattc ttattgatga tatggatact aaagaagaag aaagattatg ggatataaga
    16561 caaactgcag gcatggtatt tcaaaatcca gataatcaaa tagttgctac tatagtagaa
    16621 gaagacgttg catttggacc tgagaactta ggtatagaac caaaagaaat aagacgaatt
    16681 gtagaggagt ctttaaaaag tgtaggtatg tatgatttaa gagatagaca gcctcatttg
    16741 ttatcaggag gtcaaaagca aagagttgca atagcaggaa ttattgctat gaggcctaaa
    16801 tgtataatct ttgatgaagc aacagcaatg ttagatccat ctggaagaaa agaagttatg
    16861 aaaacaataa aaagacttaa taaggaagaa aatataactg ttatacatat aacgcacttt
    16921 atggaagaag cagtagaagc tgatagagtt gtagttatgg aaaaaggaaa gaaaatctta
    16981 gagggaactc ctagagaagt attcagtaaa ataaagatgt taaaggaaat cggtttagat
    17041 gtaccttgta tgacagagtt atctagttta ttaatagaag aaggtataaa tattagtagt
    17101 gatatattaa ctgtggatga gatggtgatg gaattatgtc aattatagta aagaatttaa
    17161 cacatatata taatgaaggg atgccttttg caagtaaggc acttgatgat gtttcttttg
    17221 agattaaaga tagagatttt gttggtctta taggtcatac aggctcagga aaatctacat
    17281 taatacaaca tttaaatgga ttactaaagc cttcctcagg agaaatattt ataaatgatt
    17341 ttaatataac agataaaaac ttgaatttaa ctgagattag aaaaagagta ggggttgtat
    17401 ttcaatatcc agaataccaa ttgtttgaag aaacgattga taaagacata gcttttggac
    17461 cctctaattt aggcttagag gagtctgaga tacataacag ggtaaaagca tcaatggaag
    17521 ctgtaggact tgattatgag ggatttaaag acaaatctcc ttttgaatta tctgggggac
    17581 aaaagcgtag agtagcaatt gctggagtta tagccatgaa cccagaagtt ctcatattgg
    17641 atgaaccaac tgctggtctt gaccctggtg gaagagatga aatttttaac ttaataaaag
    17701 acttacatga aaaaaagaat atgactataa tactatcatc acatagtatg gatgatatgg
    17761 caaagttagc aaaaacttta attgttatga atcatggtag cgtagaattt atgggaactc
    17821 ctagagaagt atttaaatca aatgctagta aattaaaaga tataggatta gatattccac
    17881 aagttttaga gcttgcattg aaattgagag aaaaaggctt tgacatcagt gaggatatat
    17941 tgactttaga agaagcaaaa caggaaatat taaaagttgt gagaggacga ggattatgtt
    18001 aaaagatata actatagggc aatactaccc aacaagttct gctattcata aattagatcc
    18061 aaggataaag cttgtagcga caattgtatt tatggtatct atatttgtag taaataaatt
    18121 ttggccttat atagtagttt tattatgttt attagctatg ataaaattgg ctaatatacc
    18181 agtcaaatat attgttaagg gtgttaaacc tcttaaatgg attattttgt ttacatttct
    18241 tataaatatt tttttcttac caggtgatga aatatggtca tttggatttt tagctattac
    18301 aaaacagggt ctaagacaag caatttttat ggcaataaga ttaatatttt tagtagttgg
    18361 tacatcattg cttactttaa caacttcacc aatagaatta actgatggaa tagagagatt
    18421 gttgaatcca tttaagaaaa ttggtttgcc agttcatgaa cttgcaatga tgatgacaat
    18481 tgctttacgt tttattccta cattgttaga tgagacagac aaaataatga aggctcagat
    18541 gtctagaggt gcagattttg aaagtaagaa tcttataaat agagcaaaga atttagtacc
    18601 acttttggta cctttatttg taagtgcatt tagaagagct gatgaattgg ctatggccat
    18661 ggaggccaga tgttacagag gtggacataa taggactaag atgagagagt ctgttatatc
    18721 aagaagggat tatatggcat gtgtatttca ggttgtttat ctgggagcaa ttatagctac
    18781 tagatttata gcaatttaat taataagtta tagatataat gaggtaaagt atgagaaata
    18841 taaaaattaa aatccaatac aatggtaaga attattgtgg atggcaaaag caacctgatt
    18901 ctttagggat acaaggtaca attgaaaggg ctatatatga tataacaaaa gaagaaacca
    18961 gtcttatagg gtctggtaga actgattcag gagttcatgc tattggtcag attgcaaatt
    19021 ttaaaattaa ttcaggtata agtatagaaa gtataccaat ggcactaaat gccaagttgc
    19081 caaaagacat atcagttata gaagcatgtg aagtaaatga tgattttcac tccagataca
    19141 gtgcaaaagg taaaacatat aaatatttag tatataatag caagtttaga aatccaatac
    19201 ttagtgagat atcatatcaa gtaaaatatg aacttgattt tgataaaatg tgttcagaag
    19261 caaaaagttt attaggaaca catgatttta aaggttttat gagttctggt tcatctgtaa
    19321 aagatacagt tagaacgata tatgatattg atataagtaa aaaagatgac ttgattactt
    19381 ttgagattag tggtaatggt tttttatata acatggtgag gattattgtt ggaacacttg
    19441 ttgatatggg tcgtggcaga attaacgaac catttttaga tataatacaa tcaaaaacta
    19501 ggtcaagatg tggacataca gctccagcac aaggattatt tctcaaaaaa gttcattatt
    19561 aacttgacac accaggctac atgtattaaa atatatgaga atgtgttaat aagtccactt
    19621 gccccggact tgacataaac ggtaaaaaaa ttttatgaaa agtagttaag gagggacagt
    19681 tatgaaaagt tatatagcta agccagctga tgtacaaaga aaatggtact tagttgatgc
    19741 tgaaggaaaa acattaggtc gtttagcaac agaaattgcg acagtattga gaggtaaaca
    19801 taaaccaaca tttactcctc acgttgatgg tggagatttt gttattgtag taaatgcaga
    19861 aaaaatagtt ttaagtggta agaaattaga tcaaaaatat tacagatacc atactggtta
    19921 tgtaggtgga ttaaaagaaa tatcttacag agatatgatg gataagaaac cagaagaagt
    19981 tatatcacat gctgtaagcg gtatgttacc taaaaataaa ttaagaagca gaatgatgac
    20041 tagattaaga gtatttgcag gtgctgagca cactcatgca gctcaaaatc cagaagtttt
    20101 aaactttaaa taagattaat aggtgaaagg aggagtttat aatggctaac gttcaatatt
    20161 atggaactgg aagaagaaaa agttctgttg ctagagtaag actagttgca ggtgaaggaa
    20221 atatattagt aaatggaaga gcattagaaa attattttaa ttatgagaca ttaataagag
    20281 atgttaaaca accattagtt ttaactggta atgaaaataa atatgatgtt atagtaaaag
    20341 ttgaaggtgg aggattcact ggacaagctg gagctataag acatggaata tctagagctt
    20401 tattaaaagc tgatttagat ttaagacctg ctttaaagaa agaaggtttc ttaactagag
    20461 atgcaagaat gaaggaaaga aagaaatacg gattaaaagc agcaagaaga gctccacaat
    20521 tctcaaaaag ataattactt atttttttgg aaaagcaaaa tcctttattt actgttattc
    20581 agaaagtatt ggtattttaa aaatgttggg ttaagttaat tattgatata taataaaata
    20641 ataagatatc ttattaggat gtttttcatt aagaagaaca tcctttttat tttgactatt
    20701 taataaaaaa catatgctaa taacatttaa attttatttt tataatattg taatttaaaa
    20761 gttt
//
LOCUS       DAELNY010000057        20656 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.057, whole genome shotgun sequence.
ACCESSION   DAELNY010000057 DAELNY010000000
VERSION     DAELNY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.057"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(9..737)
                     /locus_tag="KPE55_001773"
     CDS             complement(9..737)
                     /locus_tag="KPE55_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0492859.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            878..1603
                     /locus_tag="KPE55_001774"
     CDS             878..1603
                     /locus_tag="KPE55_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0492860.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            1663..3255
                     /gene="hcp"
                     /locus_tag="KPE55_001775"
                     /gene_synonym="priS"
     CDS             1663..3255
                     /gene="hcp"
                     /locus_tag="KPE55_001775"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG0492861.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            3626..4531
                     /locus_tag="KPE55_001776"
     CDS             3626..4531
                     /locus_tag="KPE55_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0492862.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            4655..5608
                     /gene="msrA"
                     /locus_tag="KPE55_001777"
     CDS             4655..5608
                     /gene="msrA"
                     /locus_tag="KPE55_001777"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG0492863.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            5761..6891
                     /locus_tag="KPE55_001778"
     CDS             5761..6891
                     /locus_tag="KPE55_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0492864.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFRINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(7008..7976)
                     /locus_tag="KPE55_001779"
     CDS             complement(7008..7976)
                     /locus_tag="KPE55_001779"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG0492865.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            8310..8498
                     /locus_tag="KPE55_001780"
     CDS             8310..8498
                     /locus_tag="KPE55_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492866.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            8758..9957
                     /locus_tag="KPE55_001781"
     CDS             8758..9957
                     /locus_tag="KPE55_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG0492867.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            10009..10512
                     /locus_tag="KPE55_001782"
     CDS             10009..10512
                     /locus_tag="KPE55_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492868.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            10619..10936
                     /locus_tag="KPE55_001783"
     CDS             10619..10936
                     /locus_tag="KPE55_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492869.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            11094..12053
                     /locus_tag="KPE55_001784"
     CDS             11094..12053
                     /locus_tag="KPE55_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0492870.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPSDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            12168..12953
                     /locus_tag="KPE55_001785"
     CDS             12168..12953
                     /locus_tag="KPE55_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492871.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            13245..14564
                     /locus_tag="KPE55_001786"
     CDS             13245..14564
                     /locus_tag="KPE55_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0492872.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(14639..15067)
                     /locus_tag="KPE55_001787"
     CDS             complement(14639..15067)
                     /locus_tag="KPE55_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492873.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(15158..16534)
                     /gene="hydG"
                     /locus_tag="KPE55_001788"
     CDS             complement(15158..16534)
                     /gene="hydG"
                     /locus_tag="KPE55_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG0492874.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(16535..17620)
                     /gene="hydE"
                     /locus_tag="KPE55_001789"
     CDS             complement(16535..17620)
                     /gene="hydE"
                     /locus_tag="KPE55_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG0492875.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(17656..18864)
                     /gene="hydF"
                     /locus_tag="KPE55_001790"
     CDS             complement(17656..18864)
                     /gene="hydF"
                     /locus_tag="KPE55_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG0492876.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(18879..19130)
                     /locus_tag="KPE55_001791"
     CDS             complement(18879..19130)
                     /locus_tag="KPE55_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG0492877.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(19511..20551)
                     /locus_tag="KPE55_001792"
     CDS             complement(19511..20551)
                     /locus_tag="KPE55_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG0492878.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         7735 a         2497 c         3270 g         7154 t
ORIGIN      
        1 tatctatttt atttttgaaa ataaaagatt tcaaatttat ttttgttaaa agataacaag
       61 ccttcatctc gcatcttgcc aagctctctt gacatagaac ttctgtcaac acataaaaaa
      121 tcagccattt catttcttga aaagggaatt gtaaactggt ggctacctgt ttgttctatt
      181 tgttttgaaa aatacataag caatttctcg cgagttgttt tacgagaaag taattctatt
      241 ttattgtgaa gtaaaatgtt tttttcagct attaattgta gcatattttc aacaagccta
      301 ttatgaaata cacatgcaga tgaacaaata ccagttacac ttttaaactt tataaataaa
      361 acttcgcaag aagatgttgt ggttactgta acattacttt tatgtagtct agtacatgcg
      421 aatacctctc caaataaatc tccaggcaag agttcagcta caatattcct attcccttcg
      481 atatcttcct ttattatatg agcacttcct ttaagcataa ttccaataga agtaacttca
      541 cttccaacag agaatattat actatctttt gggtaagtct tatctacaac acctaaacat
      601 tccaacattt cttcaagttc atcactttta attccttcaa atagaggaat ttttttaagt
      661 aggtctatat gcttttctaa aaattttttc aaataatcac ttccttgttg catatgcaac
      721 cgataatttg ttttcattgt actatactaa gtttataaaa gcaaggggat ataaaaatcc
      781 tactacaaga aatttgaaat aaagaaatat aggtattaaa aataaatata taagaaatca
      841 atactttaaa ttaaaaaaat tttatgaggt gatataaatg atgagaaaaa taattaaaat
      901 tgatgaggaa aagtgtaatg gttgtggact atgtgttgaa gcttgtcatg aagaagctat
      961 aggaatggta aatggaaaag caaaactttt aagagatgac tattgtgatg ggctgggaga
     1021 ttgtctgcca acttgtccaa caaatgcgat ttcatttgag tatagagaag cagcagaata
     1081 tgatgaagct gcagtaaaag caaatatgga agcaaagaaa gcacaaaaga aaactttagc
     1141 atgtggatgt ccaggttctc agtctaaatc aataaataga gaggtttcaa atagtacgtc
     1201 tataagcaat gatatagtag agataaaagg ttctcaactt aatcaatggc cagtacaaat
     1261 aaaattagta ccaactaatg catcatattt aaagaatgca agtcttttaa tagcagcaga
     1321 ctgtacagcg tatgcatatg gaaattttca taataaattt atgaaaaata aggttacatt
     1381 aatcggatgc cctaaacttg atgaagttga ctatgctgaa aaacttacag agattttaaa
     1441 agaaaatgat attaaaaata tagtagtaac acgtatggaa gttccttgct gtggtggtat
     1501 agtaaatgct gttaagacag ctctacaaaa tagtggaaaa atgataccat ggcaaatagt
     1561 tactatatct gtagatggaa aaattgtaga tggagaaatt taattaagtg aattatagaa
     1621 aaatataata aattttaata aataaaaagg gaggaataaa aaatggaaaa aatgttttgt
     1681 tttcaatgtg aacaaacagc aggaggtaaa ggttgtacta taaatggtgt ctgtggtaaa
     1741 aaggggggta cagctaactt acaagacgaa cttacaggag tgttgattgg tctttcaaga
     1801 gcaactattg gaaataagaa tagaccaaca agtgaaacag ataaaattat gatagagggg
     1861 ctattcacta ctataacgaa tgtaagtttt gatgatgaag ctataaaaag acaaattgaa
     1921 aaaactgagg tagaaaaaag taaacttgta cctagatgtt cagattgttc aacaacttgt
     1981 aatagaaatg ataactatga tatgaaggat ttatggaatg ataatgagga catacgttca
     2041 ttaaaatcat taatcttatt tggattaaga ggtatggctg cctatgcata tcatgcaatg
     2101 gtacttggtt atacagacaa agacgtaaat gaatttttct atgaaggatt aatagcagtt
     2161 ggaggagact tgtctattga cgaactttta ggtctagtta tgaaaactgg agaaataaac
     2221 ttaaaatgta tggaattgct tgatagagca aatactgaaa cttatggaac accagaaccg
     2281 actcaggtat ctatgaaaat cgagaaaggg ccatttattg tagttacagg acatgacctt
     2341 tatgatttac acaaattatt agaacaaacg aaagataagg gtataaatat atatactcat
     2401 ggtgagttat taccagcaca tgcatatcca aaactaaaag aatataaaca tctaaaaggc
     2461 aattttggta cagcatggca aaaccagcaa aaagaatttg ataatattcc tgctccaata
     2521 ttatttacaa caaactgttt aatgcctgtt aaagatagtt atagagatag agtatttact
     2581 acagaagtag ttgcttatcc aggtatggtt catattgatg aagataaaga tttttctagt
     2641 gtaatagaaa aagctcttga acttggagga tatagagaag atgttcatat gactggtatt
     2701 aatggaggag atacattaac tactggtttt gcaagaggta cagtttcatc tgtacaagat
     2761 aaagtagtag atgctgtaaa gagtggagct ataagacact ttttattggt tggaggttgt
     2821 gatggtgcaa aacctggaag aaattattac actgaatttg taaaacaagc tcctaaagac
     2881 actgttatat tgacacttgc atgtggaaaa tatagattta atgatttaga ccttggggat
     2941 ataggtggct tacctcgttt aatggatatg ggacaatgta atgatgctta tagtgcaatt
     3001 aaagtggcag tttcattggc aaatgctttt gaatgtggag taaatgattt gcctcttagc
     3061 atggtactgt catggtatga gcagaaagct gtttgtatac ttcttacact acttcattta
     3121 ggtatcaaaa atatctatat agggccttct ttaccagcat tcttatctaa gaatgtatta
     3181 gatatattgg ttgataaatt tgcattgaca cctatatcta ctcctgaaga agatatgaag
     3241 aagattttag gataaataaa tatatctata cttaaagata tttatagtta aaatattttt
     3301 aagtatgcca caattaagat aaggggttaa acttaaaatt tgattaaaaa atcaattttg
     3361 agatgaccct ttatttattt tttgtaataa ttgaaaaata aacctgcttt cagtatattc
     3421 ttttaagaat ttttgtatta aatttataat acttagatat ataatatctt atatatttta
     3481 ttttaagtat taaaggtttg aatgtaactt aaatattaaa agaggaacat tgtaatatgt
     3541 aacttgcata aaatatatat actatagtga aattattttg attaaaattt aaaatataag
     3601 gtgttttatg tgggggtata tgtatatgaa aaattctaat tttaatatat tggcatttga
     3661 tggtggtgga ttaaaagggg cattgagtat atccatactt gagaggatag taaaagagta
     3721 tccaaatcta ttaaacaata taaatatgtt tggaggtaca tctacaggga gtctaattgc
     3781 actagggctt gcgtatggtg taagccctaa agagataaaa gaactttact caattgaaaa
     3841 ttcaaagtat atttttaata aaagttatgc agaaatactc agaccaaagt atgaaaacaa
     3901 aaacttaaaa gaagttttat taagtatatt tccagaggaa ctagaattaa aagatttaaa
     3961 taaattagta atgataccaa gtttttatat aggaaatgag gaaaatgcat ggaaaccagt
     4021 gttttataac aatatgccaa attcttttac aaagactagc aaagttgtag atgtagcaat
     4081 ggcaagtagt gctgctccgg tattttttcc aacatataat cgtcatgttg atggaggaat
     4141 aatagcaact gacccgagtt tagcgtgtat aattcatgcc attgacagtg gatttaaact
     4201 taagaataca agactttttt caatagggac tggatatgta tataacagta taaaggcaga
     4261 tactactgaa tggggagcta ttgactggat aattaataaa gaacccgatt taccaattat
     4321 atctattacc ttagaaggaa attctcaaat gagtcagtta ttttctcaaa aacttcttgg
     4381 agataattac tatagattaa atcctaagat ggaaaaagat gtagctatgg atgattgtga
     4441 tgcattagaa tatctaatgt ctttaggaga aaattgtgat ataaaagatg cttttggatg
     4501 gataaagaaa aagagctttt atagaaatta gattcaattt gcaattgtat ttttacacat
     4561 gatttcattg agtaggtaaa aataataaaa tgcagattat taagttactt cttagggaat
     4621 aaataaagta taatttattt aaggagtgat gaaaatgata aaacttgcta cttttgcagg
     4681 tggatgtttt tggtgtatgg taaaaccttt tgataagtat gatggagtta ttaaagtagt
     4741 ttctggatat acaggtggtc atgttgaaaa ccctacatat caagaggtat gctcaggaac
     4801 tactggtcat ttagaagcaa ttcaaataac atatgatgat gatataataa gttatgatga
     4861 acttctaaat atcttctgga aacagattaa tcctacagat gatggtggac aatttggtga
     4921 taggggaact caatataaga ctgctatatt ttatcatgat gaagaacaaa aaaatatagc
     4981 tttagagtca agagaacatc tggaaaatag taaaatattt gatagtaaaa tagtgactga
     5041 tataattcca gcaactaaat tctacgaagc agaagaaaat catcaggatt attataagaa
     5101 aagaccatta aactacaata tgtattataa agggtccgga agatataatt ttgtaaaaag
     5161 tcattgggat ggaaacaata taaataggga agagttaaaa aagactttaa ctcctataca
     5221 atttgaagtt actcaaaatg atatgacaga agtacctttt gaaaatgaat attatgacaa
     5281 taacaaagag ggaatttatg ttgatattgt aagtggagaa gtgttatttt cttcaaaaga
     5341 taaatttgat tctggatgtg ggtggccaag tttttcaaag ccagttatag ataaatctat
     5401 cattgagaga agtgatttct cacaaggtat gtacagagtt gaagtaagaa gtgctaaagg
     5461 aaattctcat ttaggtcatg tatttgatga tggtccaaaa gaacttggag gtttaagata
     5521 ttgtataaat tctgcttctc ttaaatttat accaagagaa aatatggaag ctttaggata
     5581 tggagaatat ttaaaacttt ttgagtaatt aggaatattt attataaact tatctaaaag
     5641 gtgaaaatct aaaaaaatat tttcaccttt tgtttttttg tcaatagtat gataaattat
     5701 taattgtaat cttatttata ccaatagatt aagaatttaa ttttgaaaga gagaaaacaa
     5761 atgaatttaa aagactttaa tggaaagaga ttaaaatcag ctagaatttt tagagcaaag
     5821 acgatagaac agttgtctaa agaaactaaa ataaataaaa aagatttaaa agcttttgag
     5881 gaaaataagt atgtaccaaa tatagaaaat acattaaaat tatctaatat attaaatttt
     5941 ccaaaagagt atttttataa aaatgaaaat ataaatatcg ttgtagagga tagccacttt
     6001 aaccctcaat ctaaactacc tagagtagaa gagatttctt atagagaaaa aataattata
     6061 atacataaaa tatattcatt tatggagaat tatattaagt ttccaaaaga aaacttgcca
     6121 aatagaaaag taatgtcaga tattaatata aatgatatag aatctcttgc atataagact
     6181 agagagtttt gggatttaaa tagtactcca atagtaaata tggtaagttt gttagagtct
     6241 aaagggatta taatatcagg tatgaatgta gatagaaaag gggcaactat ctttactcaa
     6301 aaacagagaa ttagtaaaga atctaaatat ctaatatctc tgggtaatga caaaaagtca
     6361 gcatcaatta gaaattatac tttggcatgt gaacttggat atataatatc aagtgaatta
     6421 agaataccaa gtaagcaatt ttcagaagat gagtatgcct gtgcattttt attgccaaaa
     6481 gagagttttt taaaagattt gactcatcca gaagatttag attattatgt agaactaaag
     6541 aaaaaatgga ttgtacctat atctgctatg atttttagag catataatct agaaaagata
     6601 aattacaaaa aatacaatta tcttatgaat gaaatggata aaaaaggctg gttgatagaa
     6661 gaaccactag acaaaatgaa aggttcaagc ccaacgtatt taaaaagagc agttgaatta
     6721 ttgatagaaa ataaaataat gagtgtaaat agtatagtat caagtttaga ggaatttcga
     6781 ataaatcttt atccagaaga cttagaatta ttgatgggat tgaaaaaagg attgttatct
     6841 caagaggtaa ataaaaaaag taaagttata aaatttgatg gtaaaaaata ggtttatatt
     6901 ttagtattaa aattaaaagc aggtacaaat gatttaaaca ttaacttagt taaaagtttt
     6961 ttcaataagt catttacctg cttttagtgt aagaaagaat attgaatcta aagtgttttt
     7021 gaaagtgcaa tatattccct taaaacagaa caagatttat taaattcgtt ttgctcatat
     7081 tcagtcatat tgatttcaat aatttcttca ataccatctt ttcctataac acatggaaca
     7141 cctgcaaata catttctttc gttatattga ccttctagta atgtagaaac tggaagaact
     7201 tttttctcat tgtgtagtat agcttttaca atttctacta aagcagtacc tataccaaat
     7261 tctgtacaat ctttaccatt tattatactt attcctgttt ttttattttt ttcaactatt
     7321 gatggtaagt ctaaattgct ataagtactt gggttgtctt ttatcatgtc taagattggt
     7381 ttaccaccaa ttgaaacatg agaccaagga accatttgag aatctccatg ttcacccata
     7441 gaataagctt gaattgattg ttgagcgatt ccagtttctt cagataaaat ccttctaagt
     7501 cttgttgaat ctaaagctgt tccagttcca atgactttgt ttttaggaaa gccagatttt
     7561 tcccaaatat aatttgtaac tacatcaaca ggattagata tgactacaaa tataccatca
     7621 aagccagaat caactatggg ttttactata gattttatca ttccaatagt tggtcttagt
     7681 aaatctaatc tagttgtgtt ttgtctacgt aaaggccctt ctgaagagtc tgcaacactt
     7741 atcactacta tgtcagaatc tttacagtca gaaaaagttc ctttttctat atgtacttta
     7801 tgtggcagat atgaaactgc atcagctaaa tctaaagctt gagcttttga tttagattca
     7861 tctatatcaa tcataaagag ttcatcacaa actccttgag ttattaaact aaatccacaa
     7921 tgagaaccaa catgccctga acctacaata gatatttttc ttggctttat tgccatttta
     7981 aatcactccc cctaaaataa cgatacattg aaaattatag atgttattaa aagctgtttt
     8041 tggatatttt aatattctat tatttcagaa aatttattta atatgatttt atactaaaat
     8101 ttttaaaaaa gcaatgttaa tatactctct attagtgaat attaaagtat tatatgtata
     8161 ttaataatta ttaaaatatg taatgtcact gatagttaga atatttaagt gctaaaagat
     8221 gtgtataaat aataatgatg tcagtttata atgatattaa attgagatat actgtttctc
     8281 taaattaaaa aataaaggag taaatggtta tgaattatca tacaaaagaa gaattggtgg
     8341 aagcactgag agtagtatct tcaagtatta ttaattgtgg aaaaggacag aagaaatttt
     8401 ctgaggagac atctcatcat acacgtttta agaatattat agaagtaatg tatatttcaa
     8461 aatcattaat aatggatgaa attagtaaaa gagattgaag cttattttgc aatttaacat
     8521 gttttaatat acattttatt atataagaaa ataagtaaac atacatgtta taaaatattg
     8581 aaataatata caattataaa tttgttaaca ttaatatcta tatgttaaaa tatagtagaa
     8641 gatgttagta aataatagga tgagttctat tttaatacaa ttaaattaag cttagtttga
     8701 aatttgtatc taaaaagtaa agtaatctag aattaagaaa atggagatag aattataatg
     8761 gaaaaattat attatacaaa tcagtatata agagatttta cagcagaaat aattgaaata
     8821 aaagaagtag aaaataagta tcatgtttta ttagataaga cagctttttt tcctggtgga
     8881 ggaggtcagt ttggagacct gggaaaaata gagaatgatg atgtaataaa tgtgtatgaa
     8941 gatggaaaaa aaatatacca tgtattagat aaaaaaccta taaagataca taaggtaaag
     9001 tgtagtattg attgggatag aagagaagat ggaatgcatc aacattttgg acaacatgta
     9061 ttgtctggat gtttttttaa aacttttaat gcaaatacga caggttttca tttgggaaat
     9121 gagtatagta cagtagatat agaaggtaat ttagaagagg ataaaattag agagatagag
     9181 ttgtttgcca atgaaattat tcgtcaaaat atacaagtag agacattact tccaacgaaa
     9241 aaagaactaa aaaagatttg gttaagacgt gatttgccaa atactgatga agagataaga
     9301 gtaataaaaa taggtgattt agatacgaat gcatgttgtg gagtgcatcc aaaatcaact
     9361 ggtgaattga gaatgataaa aataaagaaa tgggaaaaac ataagtcaag tacaagaata
     9421 gagtttctag caggtaaaag ggcagtggat gaagtattga agagggatat atatttgact
     9481 aaaatatgta gatatttaag ctgtagtgag gaagatgcta taaatggtat tgtaaatcta
     9541 aatagtaaag tggaagaggc tttaattaaa aaaagaaggc ttgaagaagt agtatctaca
     9601 tatcaggtca aagaaatgat tgaaaaagca aataaggttg gcaatatttc ggtcattaaa
     9661 aaaatttatg atgatgaaga tttgaaatat gtaaataaga tagcaaataa aattgcagaa
     9721 tatgaaaata gtattgcatt attagctgtt aaatttaatg ataaaataaa tttagttttt
     9781 gcatgttcta aaaatttaaa agatattaat atgggagttt tattaaagga tgcaataagt
     9841 cttatagatg gcaagggtgg tggaagtaaa gtattagctc aaggaggagg taagaataat
     9901 ggaaatttag aggctacact tgattatgca tttatgaaga tagaaaaaac tctataaata
     9961 tattatataa aaaaccttag tagataaaat agatatggag gaataagtat ggagcaacaa
    10021 aaggactaca tagagttata tgaaaatgaa gaactaatag agataagaga agcacgtatg
    10081 gatgatttgg atacaatagc aaaatttaat tataatttgg ctaaagaaac tgaaggaaaa
    10141 gaacttgata tggatgtttt aactaaaggt gtaaaagcat tgttattaga tgagagaaaa
    10201 ggaaaatatc atgtatatac agtatttgat aaagttgttg cacagattat gtatacatat
    10261 gagtggagtg attggagaaa tggaaatttc ctttggattc agagtgtata tgtagataag
    10321 gaatatagaa gaaaaggtat ctttaattat ttatttaatt atataaaaaa tatatgtgat
    10381 aaagatgaaa atatagttgg aatgagatta tatgtagaaa aagaaaacat aaatgcaaaa
    10441 gcaacatatg agtctttaaa tatgtatgag tgtgattata atatgtatga atacgaagta
    10501 gtacattcgt aatataaatt tgtaatactg ttatacttat gttataaagt attgattata
    10561 ttgacgatta tatgaaataa actggatatt ataattagaa tttttagggg gtataaaaat
    10621 gggcgtttta aaaggaatgt tttatgtatt tttattttta gatttagtgt caatattctg
    10681 tttctttttc aataaaggaa aaatagcatc taataaaatt gtgtttaatg caataggagt
    10741 acttacattt gtactttgtt ttatgctttt tagttattat cccaataata atcttattgg
    10801 aaaatttatt gcttctctat tttttatatt tggagttgca ggagttatgt tgaaagaaaa
    10861 aaatttttta tatgctagac ttcttttaac tgtagttata gttttttcta cattaagatt
    10921 gttcgttatt caataaaatg tatttgtatg aaaatttatt aaagtctaca catttaagtg
    10981 taggcttttt tgattttttg tttgaaaatt aaggaaattt taaggaaatt ttaaaatcgt
    11041 cttaaatatt tattgatata cttaaaatat taatagcgaa aggagactta tcagtgatag
    11101 aaagtaaaaa gtcagaaaaa ttgttactgt caattatata cataatattt ttggtatata
    11161 ttttaatgat tataagaata attttattta gagatgtacc aatttacgct attttcaagg
    11221 gaacgtttag gaatgtaaat ttaattccat tttatacaat atatcaattt atagtagata
    11281 gtaatcttga ctttatgaaa gctactgtaa atattataga aaatataggt atatttattc
    11341 ctatgggaat ttttttacct atagtatgta aaaatctaaa taaaaaaact ataattatag
    11401 taattatact tgttagccta gcatttgaat taacacaata tatttttgct cttggaagta
    11461 gtgatataga tgatgttatt ttaaattcat taggtggcat aattggaatt aatatttaca
    11521 taaacatgaa taaattattt cccagtgata ttaacagatt taaggctata ataggtacta
    11581 gtttagtttt aggagttata ggattaggag ttatttctaa aaattatcat aacttactaa
    11641 cttttaaatt tagacctgat agaaaaatat caaaaatatt aattgaagaa aatagggaaa
    11701 taattaaaga tataaataaa gatacagtag atatagttgg gacatttgag agctttaaaa
    11761 aaggaataat aactattaaa gcaggttcta acaacaaagt aaagagacca gagaatttaa
    11821 ttgattctga tggaaatata agaatttatt taaatgaaaa tacaaaatta gtatcttata
    11881 taattaccaa agaatcaaaa atggatatag ttaaatatga ggaatttgat gtgaagaatt
    11941 tagatttatt aaggagatat gatactatta atgtttggat tgataaagaa aatcagccaa
    12001 aggataaaca tggtattatg gctagtaaat tattaattgg attgtatgaa taaaatatta
    12061 aacaagaata aaagaataac aaatataaaa aacgaaaaaa ctatttctat aattattttt
    12121 acgttgtgca taaaataaga acattagtga aggagtagag ataaattatg gagataataa
    12181 aatatatttt gtactttttt atatattcat ttttaggatg gacagtagaa agtataggat
    12241 gttctatagc aagcaaaaga ataatcaaca gaggtttttt aaatggtcca atatgccctg
    12301 tttatggttt tggagcggta attgtgatat cactattggg aagatttaat aatgtagtaa
    12361 tagtatttct attaggaatg attttaacta cgatactaga atattttaca ggatttattt
    12421 tagaaacctt atttcatgct aaatggtggg attattctga cagaaaattc aatataaagg
    12481 gtagagtatg tttaaaaaat gcaatatact ttggtgtaat gtctgtttta ataataagat
    12541 ttatacatcc atttataaaa tattttgtaa gtataattcc atatagaata ttaatttcaa
    12601 tggctataat tacaacactt tggactatac ttgatttgat tgttactatt ataacactta
    12661 aaaaattaga tataaagtta aacttattag acgatataat tacagacctt aatgatataa
    12721 atgtgaaatt ggataaattt gatagaggag aaatacaagc tttatttaaa acaataaata
    12781 acgacgaatt agaagttaga gaaaaaataa acaaaataaa tagtaagttg gaccgtatag
    12841 agtctaatat tattttacaa aaaagagtta taaaagcttt tccacatata aaacataaaa
    12901 aacaacaaga gcaactagaa cattttaaaa aaattattaa tgagaaaagg taattataca
    12961 atttaatgaa gacgaataga ataatggtat ttataagatg attttcagag gactctataa
    13021 aaatagaata gagtccttta ttttttgaaa aataatgttt atttgataaa aataaaatat
    13081 attgtttctg aataacaaat attttgaaaa ttataaaaac atatattgac aatttgtgtc
    13141 aaaaatacta acattaagta tataaagaat tttatatgag aatatcttta taaatcgcaa
    13201 taattttaaa acataaattt atttaaagag gggatgattt gccaatgagc gagataacta
    13261 gtcaaatgat aagtacagaa gaaaaaaaat atgttgctaa aactcaaaag ataccttatt
    13321 atccagtagc atttaaatca ggtgatggtg caatgctata tgattatgaa ggtaatgaat
    13381 atgtagattt tttagcaagt gcaggctctg caaatgtagg tcatggtaat aaggaaatct
    13441 cacaagcagt taaagaacaa atggatgata taacacaata tacacttgca tatttccata
    13501 gtgatcctcc tgtaaaatta gctgagaagc ttgtggaaat agcccctgga gataatgata
    13561 aaaaagtact atacagtgca actggttctg cttgtataga tgctgctata aaattagcaa
    13621 gagggtatac tggaagaaca aagataatat ctatgtgtga atcttatcat ggaagtactt
    13681 atggagctat atctatatct gctttaagta caaatatgag aagaaaaatg ggtccattgt
    13741 taccagaagt ttatcacttc cattatcctg ataaaaatag aacagctaaa gaatgtttag
    13801 atgagataga atatgctttt gcacactatc taccagcaga agaggtagct gcaatattta
    13861 tagagccaat tgcaggagat gcaggaataa tagtgcctcc agtagagtgg gtacaaggtt
    13921 taagtaaaat atgtaaagaa aatggaatat tacttgtaag tgatgaaata caacaaggta
    13981 tgggaagaac aggtaagtgg tttggtatag aaaattttgg tgttgaagca gaccttatag
    14041 ttcttggaaa atctgttgga ggtggattac ctctaggagc agttgttgga agaacagaaa
    14101 taatgcaaag tcttgatgca ccagcacatt tatttacttt agctggaaac acaacagtat
    14161 gtgtggctgc tttaaaatct atagagataa ttgaaaaaga aaacttactt caaaagagta
    14221 tagaaatggg agattatatt aaagcaggat ttgaaaaact aaaagaaaaa tacgatataa
    14281 ttggagaaat aagaggcata ggattatcta taggagttga tatagttaaa ggaaaaggtt
    14341 ctaatgaaaa acatccagat gctacagcta agatatgtta tagatgtata caaactggat
    14401 taataatgat attcttagga caatctactt taagagtgca accacctcta gtaataacta
    14461 aggagcaagt agataaagca atgaacatca tagattctgc aatagatgat tatttaaatg
    14521 gtagaattgg tgatgaagta tatgaagtaa ctcaaggttg gtaaaataaa tattaaatga
    14581 aaataggagt tatctcaatt ttagaaaatt atttctgaaa tgagataact cttttaagtt
    14641 acccacaaat aagaaaattg tttgattcat taaagactat atttcggatg tcgtttttgt
    14701 gagtatcaga gacagtatta cgagaataat tacctttatt cgtactatct gttaatttag
    14761 ttataattaa gatagctaaa agaataagaa ttactgtgta taaagcaata acatatggtt
    14821 ttcttttata tttgtatttg ttattgaaaa aagaatggcc ataattattt ttatttactt
    14881 tagatgtatt tgtattcttt ttcttgttac ctttcttttt atcatccttt tttctatttt
    14941 tagggtaagg aatgactttg tgtgtattat caagtttgat atttttattt ttttgaatat
    15001 tgttttccag gctagccttt ttttctccaa attgaatagt tttattggta ttgttgttat
    15061 ctttcaatgt taatctccct cccttaaact ttccctttag ataatattac tactaaccta
    15121 tggaatatac aataaataat atataagtaa taaaatttta taggtataaa tctctttctc
    15181 cacgctctaa ggcagtcata ttgttagata ctatgtttct tatatcttct ctttctatct
    15241 tacctatttc ttctgcgata agttttttac ccataatttt aagttcttca tctccataat
    15301 cgattaaaaa ttcatttaat gtagtaagag catttggtgt acatacatta tggatttgtc
    15361 gacttttagc taagctcatg aatctatcgc cagttctacc tttacgataa caagctgtac
    15421 aataactagg tatatatcca tcagtaatca gttctttaag cacctcaaca gggcttctat
    15481 ggtctccaac ttcgaattgt tctgtattgt caccatcttc atatgcttta tatcctccaa
    15541 cacctgttag agaacctgca ctaatttgtg atacaccata ttttagtaac tcatttctca
    15601 tttcagcagt ttctctagtg gacattatga ttccagtaaa aggaacagct aatctagtta
    15661 tagctactat ttttttgaac atatcgtcag aaactaagtg aggaaaatct tctaagctca
    15721 taccttcagc ttttttcaat ctagggaaag atatagtatg gaaacctaca ccaaattttt
    15781 cctctaagtg ttcgttgtgc atcataagtc ctagaacttc aaatttaggg tcagataaac
    15841 caaataaaac tcctgcacca acatcatcaa caccagcttc catagctcta tcaaaagctg
    15901 ttaaatggta ataatagtca ttttttatag attgaccatg catttttata aatgttggtt
    15961 tgtgataagt ttcttggaat aatatataag ttcctattcc tttttctttt agtaatttat
    16021 actcatctac acttgtagct gctatgttaa cgtttaccct tcttatattt ccgttttcat
    16081 tatatgttga ataaattgca tctaagcaat ctaagatgta atttatatca caatttttag
    16141 ggtcttcacc agcttctaga gcaagtcttt tatgacccat tttttctaat attttaacct
    16201 cttcttttat ctcttccatg gtaagttttt ttcttttaaa tttgttgcat ttactaaaac
    16261 cgcaataaac acattcattt acacagtagt ttgatacata aagaggggca aataaaacca
    16321 ctctattacc atatatttca ttttttattt gcccagctat actaaataat ctatctagtt
    16381 gttttttatc ttccacttca agaagtgtag ctatatcttt ataactaagt ttttcccttc
    16441 tatcagcctt atcaagtacc ttttcaatat catcagaagt tgaatttttt gcatcttcta
    16501 ataaagaatt aataagttca tggtttatga acatttacaa acgcctccat tcaatattat
    16561 caccgcgaga atagtcaact tcaagtccta atgacttgat gttttcttct aaactttggt
    16621 gatattcaca agcttcttga ccagtaaagg ctttgttatc gtaaagagaa tattgttttc
    16681 taaattcttg aggagaaagg tttggcataa ttacattgca accagctaaa agacctgcat
    16741 ttctaccact agggtttata gatgccaagg ctgttgtagc gggtaataaa acttttggaa
    16801 gtagaagtct tgtaattgaa agcataagta atgtcttctc taaatctcca tgtttataat
    16861 ccttaaaaat tgtatcatgg tgtggaatga aaggtcctat tccaaccata tgaggattta
    16921 actcttttag gtataataaa tcacgaacaa ggtcttcgtt gttctgaaat ggtgattcaa
    16981 ccataaatcc agcacctact tgataaccaa tttctttaag gttttttagg cattcttttc
    17041 tagttctaag ctcaatgttt ggcggatgta attttttata atgactgtct gtagcagttt
    17101 catgtcttaa aagatatcta tctgcaccac ataaaaagta ttttttatac gaatcataac
    17161 tcttttctcc tagtgataga gttatggcac aatcctcata tttatttttt atacttgaaa
    17221 caatttcaca tattttatca tcagtaaaat atgcatcttc accaccttgc aaaacgaatg
    17281 ttctgtatcc tattttataa cctatatcac aacattcaag tatttcatca agtgaaagcc
    17341 tgtatcttat gatattttta ttagaagatt gtattccaca atagtagcaa ttatttttac
    17401 aatagtttgt aagctctata agtcctctca agtagactct atttccataa tgtttatttc
    17461 tcatgtcgct agcttgttta taaagatagt ttcttaaatt ttgttcgaag ttatattcct
    17521 cattgtcgat attttctatt aaatatagta gttcatcaaa acttaatgaa ttaatgctgt
    17581 aaagtttatc tataaagtat ttaatcttat ttttagtcat attcaaatat gtaattatta
    17641 agtatagtta aatttttaat aattgaaaag ttcaagtgat ctttccaaaa taccttttac
    17701 atatgctatt aagacaccat agtttacaat aggtacattg aaagattttg ccttttctat
    17761 tctagataac attccagctc tattcatcat acaagctcca cagtgaacta caagggcata
    17821 tttatttata tcttcagtga aggatactcc agaactaaat tcaaaagtaa tgtttttgcc
    17881 agttttcttt ctaatcatat taggaatttt tactgttcca atatcatcag tttgtctatg
    17941 atgggtacat ccttcggcca tcaagatttt atcaccatct tttaaatttt ctaaagcata
    18001 agctccatta atcaattctt taaggtctcc cttttgtcta gcaaacaata tagaaaaaga
    18061 tgtaagagga atatcttttg gtgtatcttt atctacttgt ggaaatactt gagagtcagt
    18121 aataacaagt tttggttttt tgcctaaatt agatagagtt tcttttaaac tatcttcttt
    18181 tgtaactata gaaattgcac cattatccaa aatatctctt ataacttgct gttgaggaag
    18241 tataagtcta ccttttggag ctgctttatc tattggaaca actaaaacaa ctaaatcatt
    18301 tggttcaatt aaatctgata ccaatttgaa ctcagtgcta tccttaggca aaacttttat
    18361 tatttcattt tttaaatttt caattccaat tttatctgtt gatgagacag aaacaactgg
    18421 acatttaact ttatttttag ttaagtttaa aatttctgat tgattcttaa tagtatcaat
    18481 tttgttaaga attagaatat gagggatatt cttatcatta aatttttcaa ttagtgacaa
    18541 atcttcttgt gatataccaa tttggcaatc cactactagt aaagctatat ctgtcttttc
    18601 taaaacatct agtgatttac ctattctaag ttcaccaagt tcaccaacat catcaagacc
    18661 agcagtatct ataagaacac aaggtcctat aggaaggatt tccattggtc taaatactgg
    18721 gtctgtagta gttccagcaa tatctgagac tattgcagca ctttgatttg ttatagcatt
    18781 tattatgcta gatttacctg catttctttt accaaagaga cctatatgta ctcttaccga
    18841 ttgtggagta gaatttagac tcatttgtac tcaccacctt aaacttcttt ttttgatata
    18901 ctagttttta cagatacatg aggtatgttg cctaatttac cagtcaaact attaatagta
    18961 tccatttcgc caacaactgc tatacatacg attgagacac cttcttcttc gcaaggaatg
    19021 cccattcttc ctttaataat acctttaaaa cctgatacaa cctcattgaa tttaaattga
    19081 cattcttttg gctcttccaa aatagcactt ataactgcaa cctttttcat aaatatcctc
    19141 ctaactcaga aaatatgtct taaacaaatc caaaatcaaa aaacttttct ttgtaaagaa
    19201 aagtttctac aaaagaaaag ttattaatta cacaagtata atgtgctata ttaaactatt
    19261 tccttacaga ttagataact cttttctggt agggttagct tgtaaaaatt taatttaaca
    19321 acaagtaact gaagaaaatt tttatacaat ttatataaat tataataaaa attaactaaa
    19381 gaaaattgaa gttacttata taatagtagt aaattgaaat taagtctata gaaaaaccaa
    19441 tttttgtata aaaataaaaa ttaaagttaa ttatttgtta agaattgact ttggtaaaat
    19501 atataattaa ttattcttta agcatataat ttataagtaa ttttagtgta ttttcaatag
    19561 cttctatatg tgttctttca taatggtgac tattgtttgt tccaggacca acacatgcta
    19621 tttgaacatc ttctccccaa cgaatagctt ctgaagcatc tgaaccatag aaagtaaata
    19681 catcgacagc gtagtctaaa ttattgtctt ctgcaacatc gacaagcttg tttcttaaat
    19741 taaaatcata taatgataaa ttatcttttg caactatagt tacaccatgt tcactagatg
    19801 tttgacttgg tccagtaggt gctatatcta ttgctacaaa ttcttttgtt ttttcaggta
    19861 aacatttaga aacaccatgt ccaatttctt cataattact tatataaaag ttagtagtat
    19921 gcttaggtgt taaattgttt tctttaaaat atctacatat ttcaagaacc attgctacag
    19981 ctgatttatt atctatatat ctagatttta taaaaccaga ttctgtagcc tcaaatctat
    20041 ggtctaggca tacaaaatcc ccaacactta ttcctaaatc taatacatct tgctttgtaa
    20101 atactttttc atcaagtcta ataatcattg tttcttcatt tctttcagtg gcagcttttg
    20161 tttgaccata tatatgagta gaagcatact taaatacaac acttcctctg atttttttac
    20221 cttttctagt tataacagta cagttttctc cctctatagc cgcccaacat ccaccaccaa
    20281 ctttatggta tttaagagta ccatcagatg atatttcttt tactatagct ccaagtgtat
    20341 ccatatgtgc agatattgtt atttgatttg aatcatcttc accaggaaga gtagctataa
    20401 gtgcaccttt ttttgtaaga atagtgtcaa gacctaaatt ttcaaaatct tttttgattt
    20461 ctaaaactgc attttcagta tatccagctg ggcttggaat gtccaaatat tttttcatta
    20521 aatttatagt attagttaaa tttaaattca tattgtttcc tcctaaataa atatatttat
    20581 acaaattata acataaatac aaatgttctg aaaactaaaa ttaaaagaat agaatctaaa
    20641 catatttaaa aaaaaa
//
LOCUS       DAELNY010000058        20480 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.058, whole genome shotgun sequence.
ACCESSION   DAELNY010000058 DAELNY010000000
VERSION     DAELNY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.058"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            324..1517
                     /gene="nifV"
                     /locus_tag="KPE55_001793"
     CDS             324..1517
                     /gene="nifV"
                     /locus_tag="KPE55_001793"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG0492879.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1542..3467
                     /locus_tag="KPE55_001794"
     CDS             1542..3467
                     /locus_tag="KPE55_001794"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG0492880.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3489..4484
                     /locus_tag="KPE55_001795"
     CDS             3489..4484
                     /locus_tag="KPE55_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG0492881.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4810..5283
                     /locus_tag="KPE55_001796"
     CDS             4810..5283
                     /locus_tag="KPE55_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0492882.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5317..5811
                     /locus_tag="KPE55_001797"
     CDS             5317..5811
                     /locus_tag="KPE55_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492883.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5908..6429
                     /locus_tag="KPE55_001798"
     CDS             5908..6429
                     /locus_tag="KPE55_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG0492884.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            6618..6929
                     /locus_tag="KPE55_001799"
     CDS             6618..6929
                     /locus_tag="KPE55_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0492885.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6957..7643
                     /locus_tag="KPE55_001800"
     CDS             6957..7643
                     /locus_tag="KPE55_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0492886.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7875..8759
                     /locus_tag="KPE55_001801"
     CDS             7875..8759
                     /locus_tag="KPE55_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0492887.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNNFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPYIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8858..10033
                     /locus_tag="KPE55_001802"
     CDS             8858..10033
                     /locus_tag="KPE55_001802"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG0492888.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10161..11015
                     /locus_tag="KPE55_001803"
     CDS             10161..11015
                     /locus_tag="KPE55_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492889.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            11059..12294
                     /locus_tag="KPE55_001804"
     CDS             11059..12294
                     /locus_tag="KPE55_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG0492890.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12395..13336
                     /locus_tag="KPE55_001805"
     CDS             12395..13336
                     /locus_tag="KPE55_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG0492891.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13387..13746)
                     /locus_tag="KPE55_001806"
     CDS             complement(13387..13746)
                     /locus_tag="KPE55_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492892.1"
                     /translation="MDLFEVNIVLNSGTAGGMNPKLKIFNMVISTEVCHHDVTQDTLT
                     EFHSWLKTVFFEANCELIKISEMAVKKTNQEETVFWGRILAGEAFITNKGRKKINEKE
                     AYLHLIASSHIFYTIIK"
     gene            complement(13772..14236)
                     /locus_tag="KPE55_001807"
     CDS             complement(13772..14236)
                     /locus_tag="KPE55_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492893.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDIDMEEIQEAINKTSEETEEKINWTNAWGKKYPILLKY
                     QTDVNISNYAYKVNMMLDELKQEYQYNELEAMLILKDILYNVWKNRKSKRKQ"
     gene            complement(14404..14612)
                     /locus_tag="KPE55_001808"
                     /pseudo
     CDS             complement(14404..14612)
                     /locus_tag="KPE55_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            complement(14656..15009)
                     /locus_tag="KPE55_001809"
     CDS             complement(14656..15009)
                     /locus_tag="KPE55_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0492894.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            15305..16216
                     /locus_tag="KPE55_001810"
     CDS             15305..16216
                     /locus_tag="KPE55_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG0492895.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEILEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            16477..16911
                     /locus_tag="KPE55_001811"
     CDS             16477..16911
                     /locus_tag="KPE55_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492896.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGDFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            16934..18469
                     /locus_tag="KPE55_001812"
     CDS             16934..18469
                     /locus_tag="KPE55_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492897.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            18553..19920
                     /locus_tag="KPE55_001813"
     CDS             18553..19920
                     /locus_tag="KPE55_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0492898.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            19955..20179
                     /locus_tag="KPE55_001814"
     CDS             19955..20179
                     /locus_tag="KPE55_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG0492899.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         8086 a         2050 c         3756 g         6588 t
ORIGIN      
        1 acttaaatta aattttagta ggaattttat aggtttattt ttaaaggttt tttgaaaata
       61 aatctattta aaaattaaat taaaatgcaa gaaaaacata ataactttca tttttaagaa
      121 gttttcacaa aaaagaaaaa atatagtgca ataaatgata caaagtaata taatagatta
      181 caagatatga aaagctaaaa aaataaattg caaatgaaat taaaatgaat aaaaaataaa
      241 aaaataatca taagttagag gtatcaatga aaaataagac ttaatgtttt taaaattaaa
      301 agtatactaa ggggggcatt aatatgtgcg ttatatcaaa agatagagca aaggaaataa
      361 aaatagtaga tactacttta agagatggag agcaaacagc tggggttgta tttgcaaata
      421 gagaaaaaat aatgattgct gaaatgttaa gtgacctagg tgttgaccaa attgaagttg
      481 gtatacctac tatgggtgga gatgaaaaaa atgtaataaa gcacatatgt agtagaaatt
      541 taaaatcaga tataatggct tggaatagag cagtaataaa agatgttgaa gaatctattt
      601 cttgtggtgt tgatgcagta gctatatcaa tatcagtatc tgacattcat atagaaaata
      661 aattgagaac ttctagaggc tgggttttag aaaatatggc taaaacagtt gaatttgcaa
      721 agaaaaatgg attatatgtt tcagtaaatg gagaagatgc ttcaagagca gatatagact
      781 tcttaactga atttataaat gttggaaaac aagcaggggc agatagattt agatactgtg
      841 atacagtagg agttatgaat cctttttcta taaagaatgc aatagaaact ctttatgaaa
      901 gaactaattt tgatatagaa atgcatacac ataatgactt tgggatggca actgcaaatg
      961 cacttgcagg aatagcagca ggagcaaatt atgttggtgt tacagtaaat ggattaggtg
     1021 aaagagcagg aaatgcagca ttagaagaag ttttaatggc acttaagtgt gtttataaat
     1081 gtgatttaaa taatatagat actagaaaat ttagaggaat atgtgagtat gtagcacagg
     1141 catcaggaag aatacttcct acatggaaac cagtagttgg ggataatatg tttatacatg
     1201 aatctggtat acatgcagat ggagctttaa aagacccaca taactatgaa ccatttgacc
     1261 caagtgaagt aaatcttgaa agaaaaatag taataggtaa acattcggga agagctgcag
     1321 ttgtaaataa gttgagtgaa tatgaaatgt atatttctcc agaaaacgct accaagctat
     1381 taaatgctat aagagctaca tctataagat taaaaagaag tttaatggat aaagaaattc
     1441 ttcaattata ttgtgatata ctagcacatg agaaaggcac aacagaagaa gaagcagtta
     1501 gaggttcata tatttaagat ataattgttt aggaggaaaa tatgggagat aatatagttt
     1561 ataaaataat taaaaaacat atagtagatg gagaagctgt tgctggaagt tcaattggta
     1621 taaaaataga ccaaacttta acacaagatt ctactggaac aatgacatat cttcaactag
     1681 aagctatggg aatagataaa gtaaagacta aaagaagtgt ggcatttgta gaccataata
     1741 tgttacaaca aggatttgaa aatgcagacg accataagta tatacaaaca gtggcagata
     1801 aatatggagt ttatttctct aagccaggaa atggtatttg tcatcaagta tttttagaaa
     1861 gattttcaac accaggagat acacttttag gttctgatag ccacacacct actgctggag
     1921 gtgtgggcat gatggctatt ggagctggtg gattagatgt tgctttagcc atggcaggag
     1981 gagcatacta tataaaagca ccaaaagtct gtaaggtgaa tctagttgga aaattaaata
     2041 atatggtatc atcaaaagat ataatattag aagtgttaag aaaacaaact gtaaaaggtg
     2101 gagtaggtaa agtttatgaa tatggtggag aaggtgtcaa aagcctatct gttccacaaa
     2161 gagctacaat aacaaatatg ggggctgaac ttggagcaac tacttcaatc ttcccaagtg
     2221 atgaaaaaac actagaattt tttaagagtc aaggaagaga agacgcatgg gtagagctaa
     2281 aaccagatgc agatgcagtt tatgatgaag agattactat aaacttggat gaattaaaac
     2341 cattagctgc aaaacctcat agcccagaca atgtagatga agttgaaaat ataggcaaga
     2401 ttaaaataga ccaagtggca ataggaagtt gtacaaattc ttcttatgaa gacttaatga
     2461 aagtagcaca aatcctgaaa ggaaataaag tacataagga tgtgagtttg gtaatagcac
     2521 caggttcaag acaagttatg gagatgattg ctagaaatgg ggctttggca gatataataa
     2581 gtgctggagc tagaatacta gaaaattctt gtggtccatg tattggtatg ggtcaatctc
     2641 caggtactga ctctgtgtct ttaagaactt tcaatagaaa tttctatggt agaagtggga
     2701 ctttgtcagc acaagtatat ttagtaagtc cagaagtggc agcagtaagt gcaatcaagg
     2761 gagtgcttac agacccaaga gaatttgata ttaaatttac aaatttagat gtaaatgaat
     2821 ttttgataga tgatagcatg ataattaaac ctgctgatgt aggaagtgat gtagaagtag
     2881 ttagaggacc taatataaaa cctttccctc ttaatacaga gctaagtcag tctataggtg
     2941 gaaaagttat attaaagaca gaagataata taactactga ccatataatg ccatcaaatg
     3001 ctaagttact tccttttaga agtaatatac cgtatcttgc aaactattgt ttcaatactg
     3061 tagatacaga atttcctcag agagcaaaag acaataatgg tggatttata gttggtggag
     3121 ataattatgg tcaaggttca agtagagaac atgcagcttt agcaccatta tatctaggtg
     3181 ttaaaggggt aatagttaag agctttgcaa gaatccacaa ggctaacttg ataaatagtg
     3241 gtataattcc aatggagttt tgtgatgaga aagattatga aaatatatct cttttagata
     3301 atctggagat accaaatatt ttagacaatc taggtagtgg aattttagaa gttaagaata
     3361 caactaaagg aacttctttt aaggtcaaag tagaattgtc agcaaaagaa gttgatgtat
     3421 taaaagctgg tggaaaatta aactacacta aaaatcaagc taattaatat gaaaagaggg
     3481 aatattgaat gtataaagtg acacttatac caggagatgg aataggacca gaagttgcga
     3541 aagctatgaa aaaggtagta gaagctactg gagtagaaat agaatgggaa gaagttaatg
     3601 caggagaggc agttattgaa gagtatggta ctccacttcc tgaatatatt atagatagta
     3661 taaaaaagaa caaaatagct ataaaaggcc caattactac tcctgtaggt aaaggattta
     3721 ggagtgtaaa tgtaacttta agacaagcat tagatttgta tgtcaattta agacctatta
     3781 aaagttttaa ggggataaaa tcaagatatg aagatgttga tttagtggtt gtaagagaaa
     3841 atacagaaga tttatatgct ggaatagaac ataaaatagg tgattatgca gctgaaagta
     3901 taaagataat aacaagaagt gcaagtgaaa gaatcgttga ttttgcatgt aactatgtta
     3961 aagataataa gagaaaaaaa gttacagcta tacataaggc aaatataatg aaaatgtctg
     4021 atggattgtt tttagatgtg tttagagaag ttgcatcaaa acatggtgta gaatatgatg
     4081 atttgattgt agatgcagca gctatgaact tagttttaaa tccagaaaat tatgatgtaa
     4141 tggtaatgcc aaatctttat ggagatatac tatcagattt aggtgctggg cttgttggag
     4201 gtttaggtat aattccaagt gcaaatattg gtaaagattg tgcgatattt gaagctgttc
     4261 atggaagtgc tccacaaata gcaggtcaaa ataaggctaa tccaacagca cttattcaat
     4321 catcagtaat gatgcttaga tacttaggtg aatatgaaaa tgcacaaaaa atagaaacag
     4381 ctttagaaaa agtatttctt gaaggaagta aattgactgt agatttaggt ggctcagctt
     4441 ctactacaga gtttgcggat gaggtgtgta agtatattgt atagatttta tttagggaaa
     4501 aagtaaaata tagattgaag aaaagaggct ttctcaaaat agagataagc cttttttatt
     4561 ggaatgataa aatatatttg attttataca aaagagagtt cctcataaac gaaaaagtta
     4621 attttgggat atccatttta attttaaaat taattgatta atatgcaaag aaagattaaa
     4681 taaatttatt atttttaatt gaactttcaa ccaaaatatg gtaaaattta aaaaagttga
     4741 actttcaact aaaaatgaat atattgatta tgtgaaattc tataaaacag aaactatttg
     4801 actttagcca tagtagtttc agtggaggtg tgtttgatta agactttaga cagtaatata
     4861 ttaagagaag taggtacttt atctagagca gtaaattcaa taaatgatat taagtacaag
     4921 gaattaaagt tacaaaaagg acaatttaca tttttgacaa gaatctgtga aaatcctggt
     4981 ataaatcttg tggaattatc aaatatgtta aaggtagaca aggctacaac aaccaaggct
     5041 atacaaaaat taataaaggc aggatatgta gataaaaagc aggatgagtt tgataaaaga
     5101 ggatataatt taactccaac tgataaatct ttggaagttt atgagttgat aattgaagaa
     5161 gagaatagaa gtatagagat atgttttgat aattttacag atgaagaaaa acaagtggta
     5221 actaagttat tagaaaaaat gagcaaaaac gtagaaaatg aatggtttaa agtaaagaga
     5281 tagtaaataa atacattaac ttaaggagat atttttatga ttagaaaagc gaatatgaat
     5341 gatttagaaa gcattatgaa aattataaaa tctacagtgg aagaaatgaa gacttataat
     5401 aatacacaat gggatgaaaa ctatccatta gaaaaagact ttgtttcaga tattaagaaa
     5461 caagatttat atatttatga agtggatgga gaagtagcag ggtttatatg tcttaattat
     5521 gaagagccag aagaatattt aggcttaaat tggtcatcta ataaaaaagc aatggtaata
     5581 catagaatgg ctgtaaatcc aaactttaga aagacaggga tagcatctaa gttagttgat
     5641 tttgctgaaa aattagctgt agaaaacaat gtatcatact taaagagcga tacatattca
     5701 ataaactcaa aaatgaattt attacttaca aagtgtggtt ttataaaaat cggagaaatg
     5761 agttttttag gaaaggagaa atctttctac tgttatgata aaatattata aagagcattt
     5821 acaatacaag gggtaaataa tagattttca gcattagtaa gggcataata taacttagta
     5881 tatgctatta tgaaagggat gatttatatg attggatttc taaagaaaag aagaagtatt
     5941 agaaaataca aggatgttga agtagaaaaa gaaaaattag ataaaatatt aaaagctgct
     6001 cttttagctc catcatcaaa ggggcttaga acatgggaat ttatagtggt agatgataaa
     6061 gaaaagctaa ttaatttatc tcaatgtaga actaaaggtg gtggattttt cttaaaaaat
     6121 gcacctttag ctattgtaat tattgcagat aaagaaaaaa atgatgtttg gatagaggat
     6181 gcaagtatag ctgcttcata catacaactt caagctcatg aactaggttt aggttcttgt
     6241 tggatacaag taagaaatag aatgcatgat gataatatag aagctgataa gtacattaga
     6301 gaagaattaa aagtacctag caagtacagt gtagaatgta taatttctat aggatatcct
     6361 gatgaagaaa agaaagcata cagtgactct gatttagact ataaaaaggt tcattttaac
     6421 aacttttagt atataatatg ttgtattaag acattatatt tttttctatt gttaatatat
     6481 acttaaaatt gtaaaaatat atctgaattt tactaactta tctaggggtt atgtacaagg
     6541 attttaaaga taaatataga aattaatgtt tataatcgat acataacttg agtaacaaaa
     6601 tttaaggagg ttaaagtatg ggtatattta aaacaaaaat ggatgaggac tggaaagtaa
     6661 actacattaa agagtttaat gaaatgagag attcttatga gagtaaactt caaaaaaaac
     6721 agtttgaggt agatagtttg aaatcagaat tggatagact aagaagctat aaaaatagcc
     6781 ttaagccaaa agaaaaacaa attactgatg atgatataaa taatataaag agtttaagaa
     6841 gagatggctt aagctataaa gaaatttcaa atcaaacaag ttggagcaaa gcaactgtaa
     6901 gtagggtatt aaatggactt tacgattagc atatacatgg ggtgatttaa attgggatga
     6961 agtatagtta caaaaaatgc ataatagatt ctattttatt gatgtttata gttcaaatat
     7021 taaggatgat tttgaattat gtacttctaa gtcaatttga atttactctt gaaaatttta
     7081 atattataaa cttaatttct tttacattag ttggtttatc gttaatttta tttttaaaag
     7141 ataattcttt gtataacaag gttagaaata gaaaaatcac agaagctttt gaagaaaata
     7201 aaaacaatat acttattgaa aagtgtaagc tgatactttt tgttgtggtt ttatctttag
     7261 caataattgt aacttattgt actaagggat atgtactttt taatgtaact atgatgactt
     7321 tgtctgtatt gatagttcca atatttgaag agttattttt tagagaatat atatggaact
     7381 atctaagtaa ttttataaag tcaaagagta aaattatctg tataacatct atactatctg
     7441 gaatttataa tataggatac atagatgtta taagaaatta tgtaatattg tataacaatt
     7501 cgagctacac ctttgaagtt attatttcaa aaattatgat aggtactgtt tttggtattg
     7561 tacttggatt agttaagtat aggtttagag atgtagggtt ttgtatatta ttgaggagct
     7621 tatttgctat atttattagg tagatagtat aatcggttta taaggttgtt ttaaatttaa
     7681 ttttaaatca accttatttt atttgtgttt tataaagcac ttatatatta ataactaata
     7741 aatttaataa aaattatttg aatattttta aaatatataa atatgtattg acatataaat
     7801 attatgaatt acaatttata taaactaata aaaattaaat aatattaatc aacttattga
     7861 atggaggaat aaaaatgaaa tttgtgactt tttgtagtag caatgaagaa aaaataggtg
     7921 tatttaattc agaaacaaat agtatatatg aaattaatag tttaggatta agtaaattat
     7981 atactgatat gaataatttt attgaaaacg tatctacagg agatttagaa aaaataaaaa
     8041 acaattcatt tgaaaacgca aagtgttata agttagaaga agttaaatta tgttccccaa
     8101 ttgtaagacc taaaaaagat attatatgtt taggtctaaa ctataaagac catgtcaatg
     8161 aaatcccaga tggagttata aaaaacgtag ttatgccaga ttatccaatt tatttttcta
     8221 agagagcaga caaaataatt ggagtggatg ataagataag cttacatgga gatttggtag
     8281 agaagttgga ctatgaatca gaacttgcag taatcatagg taaagaaggt ataaatatat
     8341 caaaagaaga tgcttatgaa tatatatttg gatatacaat agtaaatgat ataagtgaaa
     8401 gagcacttca agataaacat gttcaatggt ttagaggaaa aagcttagac acacatacat
     8461 ctatgggacc ttatatagta cataaagaag aatttgaaca tcctttaaaa ttagatataa
     8521 gtagtgtagt aaatggagaa gtaagacaaa attctaatac agagtacttt atatttgata
     8581 tacctactat aataaatgat ttatcaagag gaatgactct aaagccagga gatataattt
     8641 caactggaac tccagcaggg gttgctatgg ggatgaatcc tcaagtatat ttaaagcatg
     8701 gcgatatagt tgaatgtaaa gtcgaaggaa taggagtttt aaaaaatata gttgattaat
     8761 ggataaatat gccatataat tttattagta cgaataattt aattaatata ggattaatca
     8821 aacttatgat taatttatta tagagaggga tgagtatatg gcaagagaac tagaattaaa
     8881 gtatggctgt aatccaaatc aaaaaccatc aaagatttat atgaagaatg gagaacttcc
     8941 tatagaagta ttaaatggaa aacctggata tataaatttt ttagatgcat ttaatagttg
     9001 gcaacttgtg aaggaattaa aagaagctac aggtctccca gcagcaactt catttaaaca
     9061 tgtaagtcca gcaggagcag cagtaggggt acctttatca gatactttaa agcaaatata
     9121 ttttgttgat gatttagagc tttctccatt agcttgtgct tatgcaatgg caagaggagc
     9181 agatagaatg tcttcttatg gcgattttat agcactttca gatgtatgtg ataaggagac
     9241 tgcaactatt atagcaagag aggtttctga tggtataata gctccaggat atacagatga
     9301 agcccttgaa atattaaaag gaaagcgtaa aggcaactat aatattgtaa aaatagatga
     9361 aaattatact cctgaaccta ttgaaacaaa ggatgtttat ggaattactt ttgaacaagg
     9421 tagaaatgaa atattaataa atgaagacct attaaaagat attccaactg ataataaaat
     9481 ttttacagat agtgcaaaac gtgatttaat tatagctctt attacattaa aatatacaca
     9541 atcaaattct gtttgttatg ctaaagatgg acaagtaata ggggtaggtg caggtcaaca
     9601 atcacgtatt cattgtacta gacttgcagg taacaaagct gatacttggt atcttcgtca
     9661 acatcctaaa gtattaaact taaaatttaa aaaggatata ggtcgtccag atagagataa
     9721 cactattgat gtatatcttt ctgatgatta tatggatgtg cttgctgatg gcatatggca
     9781 aaactttttt gaagaaaaac cagagcctct aactggagaa ggaaaaagag catggcttaa
     9841 aactttaaca ggagtagcac ttggttcaga tgcatttttc ccatttggag ataatataga
     9901 acgtgcaaaa agaagtgggg taagttttat agcacaacca ggtggttcta ttcgtgatga
     9961 taatgttata ttaacttgta ataagtataa catagtcatg gcatttacta aaaatagatt
    10021 attccatcat taaaatactg aaagtataaa gataatatag attaggtaaa aatctacaac
    10081 aagttttgtt gtagattttt ttatttgctg tataatttta aatagaactt tatttttaga
    10141 tattcaattt ggaggttgat atggctttaa atataagaaa ctatagccta ttatgtaatc
    10201 aaggaacagc taaatttaat gaagatgtag tcggtataaa tccatttggg gcatgggttt
    10261 tagatggtgc tacaggactt aatggaaaaa atttagtgtc aactgaaagt gacgcaaggt
    10321 ggtatgttca gtggtggaat aggtatttat ataaaaatat aaataaagaa gaatcattaa
    10381 agagtattat taatactgga ataatagaag taaaaaaaga gtatgaacta agattaaatg
    10441 ggtttaaggt tgaaaaatta gatttaccat ctagttctat agttgttgta aaatttcatg
    10501 aaaataaagt agagtatttt ttattaggag attgtgcttt gtttattaaa aatggagatt
    10561 ccaaagttat aaaagatagg agtatttgta aatttgataa tactgtattt gatgaaatga
    10621 gtaaactttc taacttggaa gagatggctt ttgatgagat tagaactagt gttatggata
    10681 caatagtaag caatagattg aagaaaaata caaaagaggg ctattggata ttggattttg
    10741 atgaggaagc tgtggaaaat tctatatatg gatacattga aataaaaaaa gattttcaat
    10801 tgatgctaac gagtgatggt tttacaagtg catgtgatag atataatcta ataaaagaag
    10861 aagaattgat acgtatagca gaaaaatttg gtataggata tatacataat aaagtaaggg
    10921 attttgaaga taatgactat aaagcggtta aagtgccaag atttaaagta aaagatgatt
    10981 catcttgttt atatttagat atatataatg attagactgt ataaaactat ttaattttag
    11041 aaaggaggca agtgaaaagt gaaaattctt catataataa cacaaaaacc aaatagtaca
    11101 ggtagtggta tatatttgtc tggtatgata aaagggttcg aaaaaatcgg tcataagcaa
    11161 gctgtaattg caggtataga tgtaaatgat gatgtaaatt gttttccaag tgaagtaagt
    11221 ttttatccag taaagtataa ttgtggggag ttaaattttc ctgttgtagg tatgagtgat
    11281 tctatgccat atgaaagtac aagatataaa gatttaaata tagacatgat aaatagattg
    11341 aaatatcaat ttaaagtaaa tatagataag gctatgaatg attttaaacc agaacttata
    11401 atatgtcatc atttgtacct cttaactgca tttgttaggg aaatggtaaa agatataaag
    11461 gttatgtcta tttgtcatgg aacttgttta agacagttaa ataccattga tttagagaaa
    11521 gaatacataa tagcaaatat aaggaaatta gatttaatat ttgctcttca tgaaaatcag
    11581 aaatatgata ttataaagac ttttggagta agtgaaagta aagtagttgt aattggtagt
    11641 ggttataatg atgatatatt ttataataaa aattataaaa taaaggatga taaaataaaa
    11701 atagtatttg caggtaagat atgtaaatca aagggactga ttccatttat aaaagctata
    11761 tctaaattaa aatatagtaa agatttaata gaagtcaatt ttgcaggtac tggaagcgat
    11821 atcgaatcat ataatgagat agttaaactg gcaagtaagt ctccatttaa aatgaatttc
    11881 ctaggaaagt tagaacagag agatttagct gagctattca acaggtcaca aatatttgtg
    11941 ttaccatcat tttatgaggg attaccagtt gtagttttag aagctttatc ttgtggcaca
    12001 gatgtaatca ctactgatat tcttggtgtt aaagagtgga ttggtagtga gattaataat
    12061 tctggaaaga tagaatatat ttctctacct tttatggaga aagaaggtat tccaaaagat
    12121 gaagagttat atgattttga aaataatttg tataatgcaa tagattctaa gatacaatct
    12181 ttattgaata actcaaataa gaaaacatct gtagatatgt caaaaaaaac gtgggatggc
    12241 ttagcatata gaataaatga agttatttta atggatgaat tgtgtttagt ctaaaattat
    12301 ttaaattata aaaaagtgta actattttta cactattttt aaatgatttt atattatact
    12361 aatactaaat atatttgttt tagggaggta atttatgagg attagtaaaa aggtattggc
    12421 gttaggtatt tcagcattac tgttaagtgt tagttttcca acatcaataa acgcactaga
    12481 taagattgaa aatattcaag gagtagataa atatgaaaca gctgggctta tttcagataa
    12541 gcaagatttt acaacagcag ttcttatcaa tgcagattct acaatggtag atggaattgc
    12601 tgcaagtggt cttgcaggag taaataatgc agctatttta ttaactaata aggatgatat
    12661 accagaagca acgcttcaaa gactgaatag agttactaag atatatgtaa taggtggaga
    12721 aaattctata agtaaagatg tagaaaaaat gttacttatg agaagaatgc aagttataag
    12781 aattgatggt gtagatagag tagatacaag ttataaaatt gcaggcgaaa tagaaaaaat
    12841 taaaaacagt gataagatgt ttttagtaaa tggatttaag gatgaagctg atgcagtaag
    12901 tgttgcatct gttgcttata gagatggagc tcctataata ttgacaaaag atataccttc
    12961 agcagaagaa gacacagact tagacccatg gtttggagtt tctcctgttc cagtatatgc
    13021 tattggagga gagtctacat taagtgatta catagtgagc agatatagag caactagaat
    13081 tggcggtgtt gatagatatc aaactaataa aaatgttatt gaaaagttct ataatggagc
    13141 aaaagagttc tatataacaa gtggagacga tttagtttat gctttagttg catcaccact
    13201 tgcaaaaaat gctcctgttg ttttagtttc aaataaaagt gataagagta tattaagtgg
    13261 agctagtaaa gtaacagcaa taggtataag tgataagagt attatagagc aatgtttgga
    13321 tgctgtaaag aagtaatgga gtgttattag taaaatgaat atacacattg aaaattagta
    13381 gattgttcat tttataatgg tataaaatat atgggaagag gctatcaaat ggagataagc
    13441 ctctttttca tttatttttt tgcgtccctt attggtaata aaagcttccc ctgctaaaat
    13501 tcttccccaa aatacggttt cttcttggtt ggtttttttt acagccattt cagatatttt
    13561 tatcaattca cagtttgctt caaaaaatac ggttttcaac catgagtgaa attctgtcag
    13621 agtatcttgt gtaacatcat gatgacaaac ttccgtagat attaccatat taaatatttt
    13681 caattttggg ttcattccac ctgctgtccc tgaattgaga acgatattta cttcaaaaag
    13741 gtcaatactt tagtttccaa aaaacgggga tttattgctt tcgtttactt tttcgatttt
    13801 tccagacatt gtataaaata tctttaagaa ttaacattgc ttccagttca ttatattggt
    13861 attcctgttt taattcgtcc aacatcatgt taaccttata agcataatta gaaatattaa
    13921 catctgtctg atatttaaga agaatagggt atttttttcc ccaggcattt gtccaattga
    13981 ttttttcttc ggtttcctca cttgttttat taatcgcttc ctgaatttct tccatgtcaa
    14041 tatcaaaatc aatcaattca tctaatgaca cattgtaaag tattgctaat ttttttgact
    14101 gacgaatgtc tgggactgtt tcatccgttt cccattttga aacagtctgc ctgcttaccc
    14161 ccagtttttc agctacatct tcctgtgaaa gcccatgttt cttccttgcc tgaaataaac
    14221 tatttcctaa attcattgaa attcctcctt atctgtttat gggattatta tattatttga
    14281 taagaacaaa ctctaccaac taaaattatc atatttgcta tttttattaa cataattcaa
    14341 agacgtattt attctgcaaa ttaaaatctg ctaacctctg cgaatatcaa tcattaacca
    14401 cttttatcca ttccctgttt ttgtttgtac gtaaatatct cttaattctg gtgtaaactc
    14461 ctgtaaaatc gcttcgacaa cctctttctc tttctttcaa acaacaacta aaacgtaaaa
    14521 taattttctt tcggatatac tgtgcataaa ttttttctag gttttttaaa tttgatattc
    14581 cagccaaatt cccaactgca agaactaaat atctattttg agacactatc tccttttctt
    14641 atattatatt attttttata tggaataaat ttcataagta ttatcttcct ttaatttttc
    14701 taaccaaacc atttctaaaa tatctagttc ttctaaatag tctttctctg ttttgttatc
    14761 atcatgtttt ataagctcta aaacttcaaa tgtaaatgct ttttcaccat attttttcca
    14821 ttcattattt aattctttta tcatacatga gccagcacca agctgaaatt tgagagagtt
    14881 aaatttgctt tttatatcat tactttttcc aaggtaaatc tttttagtaa tatcattttt
    14941 tattatgaat acacctgtgt caaatttcat ttctttgtat aattgtttta attcacgttt
    15001 tctatccatg attttagata cctctcttta taattgatat gtaatatgta agtgtaaaaa
    15061 tatattacat atcaattata aagagattaa ttttatatgt aaatagtttt tataaattta
    15121 attatgaatt agtcatcaat aaacaagaaa tttagctgta gaaatccatt ataaaggcta
    15181 ttataaattt taatatgcta aaaatataaa aagaatagtt gacagtgtag aaaatatgga
    15241 ataaactaaa agtgtaatag taatattaaa ttatatatta cattgaaagg tgtgaatgat
    15301 tattatgaat aaaaaaatag aattaatagg gaattttgca agtgattgga ttaggtatag
    15361 tgattatgaa tataaaatta ctgaagaaga tgaattatat atagttccaa cagaagatgc
    15421 tgtgtttacc atgtataacc catttgatgt agcagatgat ataatagtag atataattcg
    15481 cataggacaa gaggcattac aagatatatc aaaagagaca aaagaaatga aactaaagaa
    15541 agaaatactt gaatttgtaa aaaagtatgg attattgggt ttaatatatt caagtgtata
    15601 taatagagat atagttggag aagaaagagt actgatgatt gaaaagaatt atattacaaa
    15661 agagaatata ttgagtacag atgaatatgt aaacatgttt ataccatttg caagtgatga
    15721 agatgtgtat tttaaaaagt atagaagggg tatagatgta tcaaagagag aagaatctcc
    15781 aaagtttttt ggaaagagac cattgatttt agatttagta ttttcaaagt tctacactga
    15841 acaactagat tggattgtaa gttttgctaa agatatgtgt aagcatttta atcaaatttg
    15901 tgtatataaa aatacatcta atctattaac tgaaagtgtt actattatgt ctggagggtt
    15961 taatcctcaa aagataggat tttctataag ccaattagat aaaccaatta ttgcatggga
    16021 gtttgattct ttgaaaacag ctattgagac tatctatgcc tttgcaatta caagtgaacc
    16081 ttcttcttta aataaatgca agtactgtgg taaaatttat attcctacaa attctaaatc
    16141 tcaatactgt tcaccttctt gtagaaattg ctcaaatgta aagaaaagta gaagtagagc
    16201 caaaaaagat aaataaaatt aataaatata tcattgttta gattttagta aataactact
    16261 gaattaaaag ttgttatata aaattatata tctaaatgat ggtatttttt ttgttgagaa
    16321 aattgaaaag tttaattgag atagtaattt tgagttgaca aaaaccactt ttaacactac
    16381 tattataagt ggttggtaaa ataatatata ctaattcaaa tatagcataa aaaacaaaaa
    16441 aagtcaatgg aaaaaataaa aaaggagaat ttaaaaatga tatcacctat tattttgagt
    16501 tctataaatc agaatttaaa ggaaatagaa agaaatgaac tgcttgaaac taatatagaa
    16561 tctggagatt atggtctagc tctttctgaa tctgatgtaa aagatataat aaattcgaga
    16621 gataatactt taaaggggta tggaagaata gaattagata taaaagttac aaaacagtta
    16681 atagaaaata tatatacatc acaatataca aatgtggaca attatttaga ggctattaat
    16741 gatatgcaag aaatttttta ttatttaaaa aatgaaactg atgacaagat atgtgatgat
    16801 gagattatag aaatactggg tgatttttat gaaaaatttt caggcaatat ggacaatgta
    16861 agaggagaag cagacgaatt tgcaaagaaa tttaaatttg gagaagttta atatatgata
    16921 ggagagattt tgtatgttaa ataatttaag taatattaat ataaacgaaa ataatttaat
    16981 aaagaaccaa tattccatat ctctacttaa ggaatgtcta aattgtaaag tgatagatga
    17041 aagagaagta tataatatac aacaagaaat atctctaata cttatggatt tgattaaaaa
    17101 atatacaaat ggtcaaagca catcagtaaa aacagaagtt gccgaaaaac tgttgatttc
    17161 aatatggtat gctattgatg catatataaa taagcttgga gatatagaaa aaagagttgt
    17221 ggagttgact gctaagaatg ttaaaaaagt gtaccttgga ggaattgaaa tattagagga
    17281 agagttagtt aggctaaaag atttttataa actaatgatg agtagaaaat tggatactaa
    17341 ttcaatagca tataatgata ccttgatgga gttatcaagt ttctttaaaa gttatattgt
    17401 aaaatttgaa gctcaggacg ttccagtaag tatagattat ccattggcac tagatgatat
    17461 ggatgttaag ggtatttatt atgtgaagaa ttatattgag agtatagata tagaaaatag
    17521 attttgtaac ctatttaaga aaaaagatag agaaaaatta ttttatgact atggggtgac
    17581 ttataaaata gattgtgatg ttatacttgt aaatttattt gaacttatta tgaataattg
    17641 tattttttct actattttag gaaatgaagt gactaattta gaaatttcaa aatatgagta
    17701 tgaattttta gaaaaacaat ttaaaaatat gtacaataat attactgaag ataaagttat
    17761 agactctgaa gaaagtaata taattgaatt gattgaagta gatgatagtt ttaacactga
    17821 agtagatagt aatttggatt ataatgattt tgaagaaatt aacagagaaa ttagaataaa
    17881 taaaaaaata actttactac ttatggaagc aattgaagta ttaataaaaa gattaagcat
    17941 agaagatgaa gatgtaataa gatatattaa aaaatatgaa aagatattta ttgaatctgt
    18001 gctaagctca ataaaaagta acacttttaa aagtatggta actgtgagta aaaataaaaa
    18061 tgaacactta gaagattata ttgttgatga tgaaagtaaa ttggatgatg aatcgtttag
    18121 agagatattc aatgagatat tagatagtac atctataatt gaaaaaataa gaataataaa
    18181 ggaaaatatc aatgctaaaa aagactttat cgatattctt gaatctgaat gtttatttgg
    18241 agaagagtat ttaatgttgt ttgcatcatt aagtgaatta gaattggcaa tattgggtag
    18301 tgtagtattt tatgaagata ttagaatgag agaaattaat atattagaat ttatgttaaa
    18361 tgagaaaact gaaactactt tatggaagat agagtacatt aaatttatga agaggcaaag
    18421 tgaagataag atatactcca tagaagagca tatgaataaa attaattaac aaaaaattgt
    18481 gaataaatag aatgttagta tataatataa tctgtaaagc atatcaaaaa tacatatatt
    18541 tggaggaaaa ttatgaaaga gtatatcata aataatgttg aagaaataag agaagaatta
    18601 attgatttat ctaagaaaat atgggaaaat ccagaattag cctttgaaga aaagtatgca
    18661 agtagcattc aaaaagaata cctaaagtca aaaggattta aaattgaaga agtagaaaat
    18721 ttacctactg gatttatagc aagttttgga gagggtaaac ctgtgattgg catacttgga
    18781 gaatatgatg ctttaccaga actatcacaa tgtgtaagtg cagaaagaaa gccattggta
    18841 gaaggaaaag ctggacatgg atgtgggcat aacctgttgg gtgtagcagg tgtaggagca
    18901 gttgtttcta taaaaaaatt aatagaagaa aataaattta atggaacaat aaaatatttt
    18961 ggttgtcctg ctgaagaaga aggtggagga aagacagtta tgtgtataaa tggctgtttt
    19021 gatgatgtag attgtgcatt tacatggcat ccttttgata taaatgcacc ttggagagga
    19081 ggaagtctgg caaacttatc tgtaaaattt aaatttaaag ggataacagc ccatgcagct
    19141 caagctcctc ataatggtag aagcgcactt gatgctgttg aaattatgaa tgttggagct
    19201 aattatttga gagaacatgt tattgactct ataagaatgc actatgttat aactaatggt
    19261 ggtggaagac ctaatgtagt tcctggattt gctgaaagtt ggtactttat tagaggtaaa
    19321 aaagcaaaag atgcagaaca cgttcttgat agattaataa aagtagctca aggagcagca
    19381 atgatgacag aaactgaaat ggagtataag gttacagatg gaatatatga ctatatacca
    19441 aaccaatgtt tgactgattt agtttatgac aatatggttt ttgttggttg ccctaaaaat
    19501 actcctgaag aagaagaatt tgcaaaaaag ttgtgtgata ctcttactag agaagaaaga
    19561 ttaggtgtag caacatcatt acaaaatgat aaatctattg tagaaagcta tatacacaag
    19621 gaaatagttg acggtgacaa agataaagga ctagctggtt ctactgatgt tggagatgta
    19681 agttatgtaa taccagtggc tcaatttgca atggcagctt ggcctgttgg aattgcaagt
    19741 catacatggc agtcatgttc atcagcaggt tcaaatatag gattttctgc tatgataaat
    19801 tcagctaaag tactggcttg tagtgcttat gatgtattta tggatactaa aattattgat
    19861 gaagcaaaaa ttgaatttga taaatcatta gatggacaaa aatttaaacc attggtataa
    19921 ttttggtaat aaaatatttc aggggggaat agctatgaga gaaaaagttg aaaaagtgct
    19981 tgaggaaaag ataaaaccag tattacaaag agatggtgga gatgtagagc ttatagatgt
    20041 aaatgaaaat ggtgtggttt tagttagact acaaggggca tgtagtgggt gtccaggagc
    20101 gactatgaca atcaaagcta ttattgaaaa tgttttggtg agtgaagttc caggagtaac
    20161 tcaagtatta ggtgtttaat agttagcgtt tattaaatgt atgttataca taagtattta
    20221 ataaagaagt aaaaaaaata gtgaaacaat atatatgaaa aagtatgaga aagataataa
    20281 aacattatag aaataatgaa atatttcata tgtatatgaa gaaataagaa aaatagtgaa
    20341 gcaatatata tatgaaaaag tatgagaaag ataataaaac atttatagaa ataatggaat
    20401 atttcatata tacatatgaa gaaatagtaa aacaatatat atatgaaaaa gtgtgagaaa
    20461 ataataaaat actatagaaa
//
LOCUS       DAELNY010000059        19686 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.059, whole genome shotgun sequence.
ACCESSION   DAELNY010000059 DAELNY010000000
VERSION     DAELNY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.059"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(53..2095)
                     /locus_tag="KPE55_001815"
     CDS             complement(53..2095)
                     /locus_tag="KPE55_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG0492900.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(2247..3218)
                     /locus_tag="KPE55_001816"
     CDS             complement(2247..3218)
                     /locus_tag="KPE55_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG0492901.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(3219..4196)
                     /locus_tag="KPE55_001817"
     CDS             complement(3219..4196)
                     /locus_tag="KPE55_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492902.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            4415..5974
                     /locus_tag="KPE55_001818"
     CDS             4415..5974
                     /locus_tag="KPE55_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0492903.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            6080..7039
                     /locus_tag="KPE55_001819"
     CDS             6080..7039
                     /locus_tag="KPE55_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492904.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            7042..7854
                     /locus_tag="KPE55_001820"
     CDS             7042..7854
                     /locus_tag="KPE55_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492905.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(8070..8972)
                     /locus_tag="KPE55_001821"
     CDS             complement(8070..8972)
                     /locus_tag="KPE55_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0492906.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            9327..10505
                     /locus_tag="KPE55_001822"
     CDS             9327..10505
                     /locus_tag="KPE55_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0492907.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDILIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            10514..11170
                     /locus_tag="KPE55_001823"
     CDS             10514..11170
                     /locus_tag="KPE55_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG0492908.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            11170..11835
                     /locus_tag="KPE55_001824"
     CDS             11170..11835
                     /locus_tag="KPE55_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG0492909.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKKINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            11940..12716
                     /locus_tag="KPE55_001825"
     CDS             11940..12716
                     /locus_tag="KPE55_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0492910.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQESLNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            12778..14112
                     /locus_tag="KPE55_001826"
     CDS             12778..14112
                     /locus_tag="KPE55_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0492911.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            14609..15535
                     /locus_tag="KPE55_001827"
     CDS             14609..15535
                     /locus_tag="KPE55_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG0492912.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(15928..19467)
                     /gene="nifJ"
                     /locus_tag="KPE55_001828"
     CDS             complement(15928..19467)
                     /gene="nifJ"
                     /locus_tag="KPE55_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG0492913.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         6831 a         3088 c         2831 g         6936 t
ORIGIN      
        1 tattttttag tattaatttt aacatcaaaa atcaaatttt attccattat atttatgatt
       61 ctatcatcat catttcatca tagagaatat tttccaataa ctctttagcc acttctcttg
      121 atatttgctt agtttcaact aattgttgaa cactgtctct ttgactttgt atcgccatta
      181 ctctgtagta ttctactctt gcctcaaact tatcatcatt ttgaatcttt ctagtaatcc
      241 aattcaaata acgattatag aatctgattg ctattaaggc ctcatgtctg ttatcttcat
      301 cagtattttt atttataaat gatatagcag ttttagtaga aagtgcatat gcatggtgaa
      361 actctttgat tatttcttta ttctcagata ctatttttgt aattttttct tttacttctt
      421 tctttacttt tctatttata atccacatgc ttatttttaa ttttacaatt ttaaatatag
      481 atttttgaac agattttctt gattttgtaa tataaaatct atacaattta aaggctctat
      541 cagaaatttt accatcttct aatagtttca aaacagctaa taatccctgt tggtctgctt
      601 ctgcaatcat catacttata agtttattat ctggagtatt tagtattcct ttttctgagt
      661 ctaccaactg ttcatttaaa tcatgtataa ctggttgtac ttcttgtgct ttttcattac
      721 taagttttaa aatggcttct ttcaaaatta gtttatatgc ctctttatca gataataaag
      781 gttctgactc ttgtacgtta ttttttaata atcttggcag taaaactgtt gccaatacta
      841 aacttataag aataacttct gatgatataa ataaaacagt atctctcatt ggaaatacat
      901 ctttatcagc caaataaaaa ggtatagata aggcagttgc tagtgtaaat gtaccatgaa
      961 caccacaagt agcaactatt agtgagtatt tacatttaga aatactctct tttacaactt
     1021 cttcatcctt aaaacctaaa aaacctacaa aaaaattatt taaaggattc tttttaggtt
     1081 ttataaaaga attatgccat aaatataccc atataaatct tataacaaat aaaataagta
     1141 caattaaagt agaaataaaa gtcaattcca atgccatatt ttcattttta ctactaagtc
     1201 ctgtatatat acttggtaac aaaaaaccaa gcagtgtaaa gacaaatcca ttcaatacat
     1261 actccaaaac ataccaagta ttgtctgata ttatccttaa tttagtagtt gtattttgta
     1321 aatgctcttt ttcaattcca tgagctatcc ctgccattac aacagctagg attccagata
     1381 ctcctatttc ttctgctaca aaatatacaa aaagtggagt cattatttgt ataactatta
     1441 ccattggaat ttcttccaaa ttccacttgt gtaaactaag tcttattttt ataattatgt
     1501 atcctaaaat acttcctact attattcctc caaaagctgt aatcataaat tgaatccctg
     1561 cctcttctat tgaaaaaact cctgtaactg tggcaagtac tgcaacctta aatgctacta
     1621 ctccagcagc atcatttaat aaagattcac cttcaagtat actcatcata tttttaggaa
     1681 aatttaatcc ttgtgtaatt gattttactg ctactaaatc agttggagat attactgctg
     1741 ccaaagcaaa tgaaacagat aatggcatcc caggaataag aaaatgtata aagattccac
     1801 ctgcaaacac ggttattaaa actaaaccaa atgcaagaag caaaattggt tttctgagct
     1861 ctaatagctc ttttctcgat acattttgtc cttcactgaa tagtaatgga gctatgatac
     1921 atattacaaa tatttctgga ttaaatgaaa atgagaattt aaatggcaaa atagacaata
     1981 taatccctag tacaatttga tatattgcaa gaggaatatt actccacttt ttgctaaata
     2041 cagttgaaat cgttgtacaa accaataatc ccaacaatgt tactaaaatt gacataacta
     2101 cctctctttt caataataat attatagatt aaacttatat atcaactaac ttaatattaa
     2161 cagcatgaaa tcccatctta atcaaaaaaa ttttcagttt attctattat tcttattata
     2221 tacatcaaat ttaatttttg aaatatctat actagatgac atgcaacaaa atgatttctt
     2281 gatatttctt ttaaaactgg aatctctttt ttgcatattt ccttacaata agggcatctt
     2341 gtatgaaact tacatccact tggtggattt gttggagatg gtatgtctcc ttttaatata
     2401 attctatttt tcttaattgt agggtcttgt attggtactg cgctcataag cgcttttgta
     2461 tatggatgtt gagaatgtaa gtaaagttct cctttagttg caatttctat tatagaacct
     2521 agatacataa cacaaactct atgtgcaata tgatttacta cactcaaatc atgagatata
     2581 aataaatatg aaagtttata tttatcctgt aaatccataa gtaaatttat aatttgagat
     2641 tgaatagaaa catctagtgc agaaacaggt tcatcacaaa ctataaattc tggatttaat
     2701 gctaatgctc ttgctatacc tattctctgt ctttgacctc cagaaaactc acctggtttt
     2761 ctatttaaat ggtacttaga aagaccacaa ctttctacaa catttaatat attatctttt
     2821 atttcattct ttggcactaa attatgttct ataactgcct cggatattat ttgttccaca
     2881 tttaatcttg gatttaatga actatatggg tcttgaaata tcatttgcat tttgggtctt
     2941 aactttctaa gttcttcctt ctctaactta tatatatcta caccattaaa ttcaacttta
     3001 ccttcagttt tttcaattaa tctaagaatg gttcttcctg cagttgattt tccacatccc
     3061 gattctccaa ctatagccaa agtttctcct ttttttaaat caaaagaaat tccatctact
     3121 gcctttacac atttaatttc cttatcaaat aatccagatt taacttcaaa gtacttttta
     3181 agaccttcta cttttaataa actctcttct tttttcaatt atatctcctc ctttttatat
     3241 aaccaacatg caatactatg accactattc aattctatta aaggaggtat ctgagttcta
     3301 catttatcac atactttttc acatctatca ctaaaataac atgaatctgg catcccaatt
     3361 ggatttggta cttgtcctgg aatcgaatac aatcttttat ctttgttttt tccaagagca
     3421 ggctttgatt ttagaagtcc aatagtataa ggatgttttg gatttttaaa taattcaaca
     3481 acctctgcca cttctataat tttaccagca tacatcacta atactttgtc acacatttct
     3541 gcaactactc ccaaatcatg tgtaatcatc attatagatg tgtttaattt ttcttttata
     3601 tttttcatta tatctaaaat ctgagcttga atagtaacat ctaaagctgt tgttggttca
     3661 tcagctataa gtaattttgg attacatgat aaagccatag ctatcattat tcgttgtctc
     3721 ataccacctg ataattcatg tggatatgaa ttatatattt cttcagctct tggtatttct
     3781 accagtttta tcatatcgat tacctttttc ttagcttctt cctttgataa atcttgatgt
     3841 atcataatac cttcaatgat ttgacttcca acagtaaacg ctgggtttaa agatgtcatt
     3901 ggttcttgaa aaatcattga tatctcattc cctcttattc ccataagttc atcttcattc
     3961 atctttaaaa tgtctttgcc tcgaaagatt atttctccac cttctatttt tgatgttgga
     4021 agtaacttca tcaaagacat ggctgttaca gacttaccac atcctgattc tccaactatt
     4081 ccaatagttt caccttcatt tatattaaaa cttacttcat ttacactctt tacaatccct
     4141 ttatctgtat gaaaataaac ttttaaatct ttaacctcaa ttagtgcttt actcattatt
     4201 ttctcctctc agcaaaatta cttaagataa catacacctt ttgattgtat tttataaaaa
     4261 tttaaattac aacattttct tacattttat tatttttttc tattaattat aataatataa
     4321 tagttaattt ctatatatat tgatttaata atgattacta ttatacaata aaaaatatgt
     4381 gttaaatatt attttatgga ggttttataa gaatatgaaa tttaaaaaat tggcatcatt
     4441 aatcttagtt tcatcattaa tgcttacatt tacagcctgt gcttccaatg ataaagacaa
     4501 gtctagtgga agcaaatctg gtggaactgt tgtaatacct atggcaagtg acccagatat
     4561 aatcaatgga gcttttgcaa acgttaaaga agactctctt gcatcaaata tagtttatgc
     4621 tcctctttat acttatgaaa aaggaaattt agtaaattat ttagcagaaa aagtagattt
     4681 taaagatagt aaggaattaa ctataaaatt aaaatccaat ttaaaatggc atgatggaaa
     4741 gccaataact gctgaagatg tcttatttac atttaataca gttttagacg aaaaacaaaa
     4801 ttcaccatca agacaatact tattggtggg tgagaaacca gttaaagtag aaaaaattga
     4861 tgatttaaca gttaagatta ctttaccaac tgcaagtgaa tctttcttat atggtatatc
     4921 taaaatttct ccaataccaa aacatgtgtt tgaaggagaa tcaaatatag ctaagtcaga
     4981 aaaaaataat aatccagttg gttctggtgc attcaagttt aaggaatgga aaaaaggtga
     5041 aagcatagta tttgaaaaga atgcagatta ttttggtgga gaacctaagg cagattctat
     5101 agcgctaaaa ataattccta atgaagcatc tcaagaagca gctttaaata atggcgaaat
     5161 ttcacttatg aagacatctg ctgaaggata tgaaaaggca aaatcaaact caaacttaca
     5221 aacttatact tatagtgaag aaagattaaa ctacattgta tttaaccaaa atatatcaaa
     5281 catggctaac aaagaagtaa gacaagcctt atcatatgcc ttaaatagaa atgaaatgat
     5341 agaatcagca tatggaaaag aaggttctgt acctgctaaa tctatattag ttccagaagc
     5401 agacttctat acagaagaag gtgttgaagg atatgaccaa gatacaaata aggctaaaga
     5461 tttattggat aaaagtggtg ttaaaattga taaattaaaa ataggttata acacaggtag
     5521 atttgggcat aaaaactatg ccttagtagc tcaacaagag ttgaaaaaaa ttggtataga
     5581 agctgaaata gttccttatg aaagtaaggc attcttcaat atactattta gtaatagcac
     5641 tgaatgtgat atgtatgtaa atggttatgc ttggggatta gaaccaaacc catatagagg
     5701 tatgtttgaa actggtcaat attgcaacca aactaaatat tctaatgctg aaatagatgc
     5761 tttatgggaa aaaggattta ctgagttaaa caaggaaaaa cgtgaagaaa tatataagca
     5821 aatacaacag gatatatcta aggatgcgcc tatttacact atagattatg aacaaaattt
     5881 aatggcagct caaaaaaatc taaaaggaat aaaagatgct aaaccatctc ctgcaatatt
     5941 atttgaagat tggtctaaac tgtatgtaga ataattgatg taaaatttta tattattaat
     6001 tagaaaagga gatgtctaaa aataatattg ttttattatt ttaaaacgag acatctcttt
     6061 taattaggag aatttactta tgttaaaatt aatattgaat agagtaaaag ttttaatacc
     6121 tatgttaatc ttaatatcta tattatcatt tggactttta gaattagctc ctggtgaccc
     6181 agcagatgca tatataaatc ctttaatgtc ttcacaggat attgaaaata taagggttaa
     6241 tatggggctt gataaaccag tatatataag atatttaaac tggttgaaaa acactttaag
     6301 tggaaactta ggtatttctt atataaacca tatgcccgtt acagaacaaa ttatggaaaa
     6361 aatgggaaat acatttattc ttatgggtac ttctttgata ttttcaatat tggtagctat
     6421 acctcttgga atatttttag ctgtaaataa aaattcaata actagtaaag tatcatctgt
     6481 ttttaattat ataggtgttt ctataccttc tttttggata ggtatgatac tcatttcaat
     6541 tttttcagtt aaacttaaca ttttcccatc aggaggtatg catactattg gaaatgatag
     6601 tattggagat ttagtaaaac atttagtttt gcctgtaata actttaggtc tatataatac
     6661 tgctattttt acaaattatg tagaagctag tgtaaatgaa caattaaaaa aacaatatgt
     6721 tattacagca agagcaaaag gattatcaga aaaagtcata ctatttaaac atgtcttgaa
     6781 aaattcactg acatcattgg ttacaatcct aggcatgagt atacaaaaat tagtcactgg
     6841 tgcatttgtt acagaagtag ttttctcttg gccaggtatg ggaagattaa tgattgattc
     6901 aattttttca agagattata ctgtaattat ggcaataact atgttatcag cactattttt
     6961 aattttaggt aatttagttg ctgatatact atacttatta attgacccta aaataaaatc
     7021 tagcaaagga ggattctagt tgtgtttaca agtatcttaa aaaaattaaa aaaagataaa
     7081 tcctttgctt ttgcatgtat tactcttagc ataataatca tattaattat aacttctttt
     7141 atattaaata tagactctaa tgcaattgat actacttcta aacttcaaga acctagctta
     7201 aagcatatct ttggtacaga cgaacttggt agagattact tctctagagc tctttatggt
     7261 ggaaggattt cacttagtgt tggtatttta tcaatgctta tttctgtagt atttggtact
     7321 gctgtaggtg ttattagtgg atatattgga ggtaagctag accaattctt gatgagatta
     7381 gtggatatgc ttatgagtat accaagtttc ttagttttga ttgtattaaa tacttatcta
     7441 acacctaaag tttccactat tatactaatt attggattct taagttggat ggaagtttca
     7501 agaattgtac gtggcgaaac attaaaaata aaggaaaatg aatactgtct agctgctaaa
     7561 gctactggta tcaaaactag aaatatacta ttaagacata tagttttaaa cttaaaagag
     7621 actattgtag ttgctggttc gttaaatatt gctaatgcta tcttaactga atctgctctt
     7681 agttttttag gtcttggaat ccaattgcct cttgcatcat ggggtaatat gcttcaagat
     7741 gctcaaagac atatttttga caagttatat ttagctgtat ttccaggatt acttatattt
     7801 ttaacagtca tgagtttgac tataataagc aaaaaatttt ctagtaatac ttaacaaatt
     7861 ttatatagaa aataaaaaca gtgattatct aaagatatac actgttttta tttttcgttt
     7921 aaaaaaatac atattttgta tattataact ttaaatacta ctctaccatg ttatgtaaag
     7981 cctattattg ctctcaaaca tatagatgaa aaactaatag tttattataa taaaaattga
     8041 tttatttatt tgttatttta ataacatttt taagagttct ttttactaaa gaaatctata
     8101 ataaatttaa atacatgttg gtctacatgg ctataacgtt cttttttagg tctacatagt
     8161 ataactttcc atgtaatagg atgattaaaa ggcttttcta taattttgct attttcaaaa
     8221 aaatcatgaa ctggtgatgg tagaattgtt ataaaatcag aatttgtggt tgatagcaac
     8281 aaaaaatccc atgaaccaga cattatatat ctttttaact taacgttttc atcagtaaat
     8341 ttttgcatta gcttatgatg tatcataaaa gtaggattaa atattgcaag taactgctca
     8401 ttcaaatcgt tccaatctat cttatccttc ttagcaagtg gatgatttat atttaaaaat
     8461 gctgtaagtt catcttcttg aagcacatgt tcagttacta cacttttatc aattttatgt
     8521 ggatgtaaca atactgcata atctaattcc tgtaagataa gcattctatt taagtcatat
     8581 gcacccttct ctactatatc aaattctata tctggattat ttgcaactaa ctgtgcaact
     8641 acatctgaaa atgctattcc aagtataagt ggaggtattc ctattctaac ttttccttta
     8701 aattgaactg aatcttctct taaatcttcc atcatatttt tgtattcatt tatgatatgt
     8761 tctgcattta cataaaatct ttctcctgct ggtgttaacc catttaacct gcctttatac
     8821 ctttcaaata aatatacatc ttcactttct tcaaaatttt ttataatttg acttaatgct
     8881 ggttgagata tgtgaagtgt cttagatgct attgataaat taaaaccaga tttcactatt
     8941 tcaacaaaat atttcaactg ttttatatcc attttaaacc cccttcccca aaagtataat
     9001 ttagattata ttttttcttc taatgatatt ttataggaaa atcaacataa tttcattaat
     9061 attttaacaa aatatttttc taaatctact ataaattttt atcctataaa tttttcttat
     9121 acactatata aaaaattaga cttagacaaa acgctttttt cgccgtaatc tataattaga
     9181 aacaggaaaa cctaaagcat taggaataaa agtaattgaa atacttaaaa acaagcttgt
     9241 attaaaaaag tactttatta tagtgttccc taatttcttt attcagaaaa tttttaatta
     9301 tcattcaaga aaggtaacta ttagatatga aagatgtagt aatcgtatca gcagtcagaa
     9361 cacctatagg aagttttgga ggtgtattta aaaatacttc agctgttcaa cttggaacta
     9421 tcgctgttaa agaagcgata tcacgtgtaa gacttaattt atctgaaata gatgaagtaa
     9481 taattggaaa tgttcttcaa acagggcttg gacagaatgt tgctagacaa attgctataa
     9541 atgcaggtat accaaattct gttccaagct acacagtaaa taaattatgt ggttcagggc
     9601 ttaaaagtgt ccagctagct gcccaatcaa tcacatctgg tgaaaatgat gtggtaattg
     9661 caggtggtac tgaaaatatg agccaagctc cttatatcgt tccaactgct cgctttggta
     9721 gtaaaatggg tgacataacc atggtagata gtatgctaac agatggcctt attgatgctt
     9781 tcaatcagta tcatatggga ataactgcag aaaatattgc aactaaattt gaatttacta
     9841 gagaaatgca agataaattg gcattagaaa gtcaaaataa ggctgagaat gctataaaaa
     9901 acaaccgctt taaagaagaa atcgttccag tagatatttt aatacgtcga ggaaaaatag
     9961 aaactattga taaagatgaa tatcctaaat taggtatgac atttgaaggt ttagctaaac
    10021 ttaaacctgc attcaaaaaa gatggtactg taacagctgg aaatgcctca ggtattaatg
    10081 atggtgcggc tatgttaata ctaatgagcc aacaaaaagc agacgaacta ggtattagac
    10141 cacttgctaa aataaaatcc tatgcttcag ctggtgttga acctgaagtc atgggtactg
    10201 gtccaatccc tgcaactaga aaagcactga aaaaagctgg acttagcatc aatgatatag
    10261 acttaataga agcaaacgaa gcgtttgctg cccaagcatt agctgttaaa aatgaacttc
    10321 aaatagattc atcaaagtta aatgttaatg gtggtgcaat agcattagga catccaatag
    10381 gagcaagtgg agctagaata cttgttacct taatttatga aatgcaaaaa cgtaaagttg
    10441 aaactggact tgcaacactt tgtattggtg gtggtcaagg aatatcaatg gtggtttcac
    10501 gataggagga aatatgaata aaatagttag cattgatgaa gctctatctc atgtaaaaga
    10561 tggagataca ataatggttg gtggattcat ggccaatggt tcaccagaaa atttaattga
    10621 ttatttatgt agtaaaaata taaaagattt gacactaatt tgcaatgata ctggctttat
    10681 tgacaaagga gttggcaaaa tggttgtaaa taagcaattt aaaaagataa ttgcttctca
    10741 tgtaggatta aataaagaaa ctggaagaca aatgaacaac aatgaaacag atgttgagtt
    10801 agttcctcag ggaactcttg ctgaacaaat acgtgcagct ggttatggtc taggaggtat
    10861 actcacaccg actggtattg gtactcttgt tgaggaggga aagcaaaaaa ttgttgtcga
    10921 tagcaaagaa taccttcttg aaaaaccaat ttatgcagat gttgctcttc tatttgcatc
    10981 aaaggtcgat aaagcaggta atctagttta taaaggctct atgaacaatt ttaataattt
    11041 aatggcatct gctgccaaaa ttacaatagt tgaagcagat gaaatcgttg aaattggtga
    11101 tattgaccca aatgaagtta atacaccagg tatttttgtg aattacatcg ttgaaggagg
    11161 taatttataa tggataaatt agaaatgcaa gaatatattg caaatagagt atctaaagaa
    11221 ttaaaagacg gagccgttgt aaaccttgga ataggtctac caaccaaagt agcaaattat
    11281 attccggata atgtaaacgt tatcttacaa tctgaaaatg gatttttagg tttaggttca
    11341 gccgaagatg gaaattcaag tgatgaaact attgtaaatg caggtggtca accagttact
    11401 atacttcctg gaggatgttt ctttgatagt gctacttctt ttggaataat cagaggaggt
    11461 catgttgata taactgttct tggcgcactt caggttgaca aatatggcaa tatagcaaac
    11521 tatatgattc caggtaagat ggttccaggt atgggtggag ctatggacct agtaactgga
    11581 gctaaaaaag ttattgtagc aatggaacat acttcaaaag gttctgcaaa aatattaaat
    11641 aattgtactc ttccacttac tgcaacaaat gctgtagatt tgatagttac tgaaatggga
    11701 gttatggaag taacatcaga tggaatttta cttaaaaaaa taaatccagc tttcacatta
    11761 gatgatgtta tttctgctac tgaagctcct ttaatattgt cagattcatt aaatggaaaa
    11821 atatctgttg tttaaaataa ttaaaactag aaaataaaaa tctcttagac gtattacata
    11881 taaatttgta ttgcttaaaa tattaatata aaaataaaat aaaattggag gaaatgaaaa
    11941 tggttaaaga taaagttgta ttcgtaacag gagctgcaag tggaataggt aaacaaatag
    12001 ctgaatcgtt cttaaaaaat ggtgctaaag taatgttctc tgatataaat caagaatctt
    12061 taaataatac cataacagag ttacaaaaaa aaggttatga ttgcatgagt gtaaagtgtg
    12121 atgtaactaa ggaagaagaa attaatcacg ctatagacaa aactgttgaa aagtatggca
    12181 gattagatat attaataaac aatgctggac ttcaacatgt ttctatgata gaagatttcc
    12241 caactgataa atttgaattt atgataaaaa taatgctgac agcacctttt atagctacta
    12301 aaaaagcatt tcctataatg aaaaaacaag gctttggtcg tataataaat atggcctcaa
    12361 tcaatggtgt aataggattt gctggaaagg ctgcttataa ctcagcaaaa catggactta
    12421 ttggactaac aaaagttgct gctcttgaag ctgccgattc tggtataaca gttaatgctt
    12481 tatgccctgg atatgtagat actccacttg tcagaggtca gttcaatgac ctttcaaaaa
    12541 ctcgtaatat accattggaa aatgtcttag aggaagtttt atacccactt gtaccacaaa
    12601 aacgcctatt agatgttcaa gaaattgccg actatgctat gttcttagca agtgataatg
    12661 ctaagggtat tacaggacaa gcttgcttac tagatggagg atatactgca caatagttat
    12721 tatatttagg gcttgaagaa agtattcttc aggtccttct actaaggagg gatatttatg
    12781 agtattatcg caccaattgg tataattatt ggtatcatag ctattatata tttttcagtt
    12841 aaagaaattc atataactat agctgctcca atagcaactt taatagttgt tattttaaat
    12901 aaaatggatg ttgtaacgtc aatgctaggt gctgacaaaa acaattatat gggtgcactt
    12961 ggaaactata taatgagtta ttttgcaata ttcttattag gctcaatact tgctaaattt
    13021 atggaagaaa gtggagctac tgtatcaata gctgatttta ttttaaataa atttggctct
    13081 aatcatcctt atagagtttt agttgctata tttatagtaa gttttatatt aacgtatggt
    13141 ggtataagcc tatttgtagt tatgtttgcg gtcattccac ttgcaagaac tctatttaaa
    13201 aaacttgata tttcttggaa ccttattcag ataccacttt ggcttggtat tgcaacaatt
    13261 actatgacta taattcctgg aacaccagcc attcaaaatg ttatacctat tcaatatcta
    13321 aatacgtctc ttactgcagc tactatacca agtattgtag gtagtatagg ttgtgctaca
    13381 tttggacttg tctatatgaa atatgctcta aacaaaagta ttagaaaagg tgaaacttat
    13441 gcaacttaca cttcagaatt agaagaagaa tctttagata gagaattacc aggatttttt
    13501 gcaagtattt ttcctctatt agcactcgta attatagcta taactggaag tacttttggt
    13561 tcagaattta tgaaaaaaaa tgtaatttac atagctttaa tagttgcaat tcttttatct
    13621 gcttttttat tccataaatt tctatcatct aagatacaga cattgagcat aggtgctagt
    13681 ggttctatag gtcctatatt tgctacatct tctgcagttg catttggagc agtaataatg
    13741 gctgcacctg gttttgatgt atttagcaaa cttataatgt caataccagg tagtccactt
    13801 ataagtctta ctgttttgac ttcaacaatg tcagcaataa cagggtcctc ttctggtgca
    13861 ttaggaatag taatgccaaa ctttgcagat tattatttaa gtactggtct aaatccagaa
    13921 ttaattcacc gtgttgctac aatagcctca aatatactaa ctatagttcc acaaagtggt
    13981 gtttttctta cattcttaag cttatctaag ttaacacata aaactggatt taaagaaact
    14041 ttcattgtag ttgcagttgg ttcactaata gcagaaatta ttgtaattac attaggatta
    14101 ctaatgtatt aatataaaac aaattaaaat tcataatatg aaaggtggtg tctcaaagtg
    14161 aactttttac ttaatgagac actcttttta tatgaaataa cataagccta tcatttcaac
    14221 aataaaaaag aaccttatct ctattttgag acagaccctt tttatttctt aaagatttaa
    14281 ataattataa tttgttgcta atacgaaagc atctactgaa taaatttaac caaataaaaa
    14341 aaaatttatc ataactataa atttacgtta tggaattatt ataaaaatat tattttctct
    14401 aactaagtaa ttccatacta tacaggcttt taatacaaat tgtcaaacac actgaaatat
    14461 acctatttat tccatatata tcttttttac atatctttac agtaaaaaaa gtcttaatct
    14521 tatgttagta tagattcacg ggtaaccccc tacactaaag gttacaaatc agtaacattt
    14581 ttagaatttt tagattaaga ggtgtttaat gatgagaaga aatataccaa caaaattaaa
    14641 aactatatta gctttaggag tgttaacttt agcaacagct tcaagtgctg ttactgcaag
    14701 tgcttcatca cttgaaaata atgtaaatat aagtaacaaa agtttagtat atagtaatac
    14761 taaatacaag ttttctgcaa aaccaaattg ttctgtaaaa ccagaactta agcctgataa
    14821 agataactct tgtaaggata agaatcataa tgacaagaat tgcaacaaca ctaataaacc
    14881 tgaggacaac aataattcag actctactaa taagcctgat aacaataaac ctgagtctaa
    14941 taaacctgaa gacaacaata attcaggctc tactaataaa cctgataata ataaacctga
    15001 gtctaataaa cctgaagaca acaataattc aggctctact gataaacctg ataacaataa
    15061 acctgagtct aataaacctg aagacaacaa taattcagac tctactaaca aacctgatga
    15121 taataacaac tcaggctcta caagtgaaag tttttcagct taccaaaaag aagttgttga
    15181 cttagtaaat atagaaagag ctaaagctgg attaaatcct cttactcttg attcttctat
    15241 atcaaatgtt gctacaaaaa aatctcaaga tatgattgat aacaactatt tttcacacaa
    15301 ttctccaact tatggttctc catttgacat gttgaaaaaa tttggaataa gctataagac
    15361 agcaggtgaa aatatagcta tgggtcaaaa aactcctaaa gaagttgtaa acgcatggat
    15421 gaattctgaa ggtcacagaa aaaatataat gaatccaaat ttcagtaaaa taggtgttgg
    15481 agtagcacaa aaaagtggtg gttcaattta ttggacacaa atctttgtag gataaaatac
    15541 tctttttcca tacatatcaa aagagtatct ctaagtgata aaaaacacaa atgagatact
    15601 cttttgtatc tattttaaaa ttatcaaatc caaatataca tactataaat attatttgtt
    15661 ctcttaattt tagtgtgtag agcaatatat aaaaagtatt ggcaagatga aaatggaaaa
    15721 cttaaaaaag atatttaata ggtaaaaaca aaatcttaat atcttcataa aaaaaggctg
    15781 tctcaaaata gagataaggc ctttttcatt gttgaaatga ttaaaatata tttgatttca
    15841 tacaaaaaga gtgcctcatg aactaaaaag ctcactttga gacactctct tttatcaatt
    15901 ctatataagc attataatac tttaatatta ttcagctaat tttttgtatc cttcatatct
    15961 ttctttagca tcagcttcag ctttagcaaa taatccatca gcttcctctg ggaattgttt
    16021 agctatagca gaatatctta cttgtgcaag tatgaaatct ttgaagcttg cagttggttc
    16081 ttttgaatct aaagtgaatg ggtttttacc ttcagctttt acttctggat tgaatctgta
    16141 taaatgccag tatccgcaag caacagcttg tgcttcatta gctacagttc ttcccatacc
    16201 ttcttttaat ccatggttta tacatggagc ataagctatt attagagatg gcccatcatg
    16261 tttttcagct tctaaaacgg ctttgataaa ttgattttta tcagcaccca tagcaacttg
    16321 agctacatat acgtttccat atgtcatagc catcattcct aaatctttct ttctagattt
    16381 tttacctgaa gcagcaaact tagccatagc agcagctgga gtagctttag aagcttgacc
    16441 tcctgtattt gaatatactt ctgtatcaaa tacaagtacg tttacatctt caccagatgc
    16501 aagaacatgg tctaatccac cataaccgat atcatatgcc caaccgtctc cacctagtat
    16561 ccattgagat ctcttaacta gataatcttt tctatctttt atagctttta ctaattcttt
    16621 agctttttca tctgtacaat cacaagctgg tttttctaat aattcaacaa ccttagcact
    16681 tgctacttta gaagcttctc catcatttct agaatctaac cactctgtaa atactgcttt
    16741 agcatcttca cagatatcca tagaaactaa ctctgtcata gcatctacta ttttatttct
    16801 tatttgctta acagctgtgt acataccaaa tccatattca gcattatcct cgaataaaga
    16861 atttgcccaa gatggacctt taccttcatg gttacaagta taaggaattg atggtgcaga
    16921 agctccccat atagaagaac atccagtagc attagctatc atcattctat ctccatataa
    16981 ttgtgtaact actttaatgt acgcagtttc tccacatcca gcacatgctc ctgaaaactc
    17041 cattaatggt tgtctaaatt gacttccttt aacagtattt gcaggcataa catcaacttt
    17101 tggagctact tcatcaggat ttactgcaaa tgcccaatta tctaactctg tatcaagttg
    17161 agtatctatt ggcttcataa ctaaagcttt ttctttagct ggacatatgt cagcacagtt
    17221 tccacatcct gtacagtcaa gtggactaac ttgaattcta tattttaatc cttctaatcc
    17281 tttacctaat gcttttttag cttcaaatcc ttctggagca ttagctaatt cttcttcagt
    17341 aactaatact ggtcttatac atgcatgagg acatataaaa gaacattggt tacattgaat
    17401 acagttatct attatccatt caggaacatc aacagctata ccacgttttt cataagctgc
    17461 agtaccacaa gggaatgttc catcttctat accattgaat gtacttactg gtaagttgtt
    17521 tccttcttgt gcagtcattg gtcttagtat attttttata aattctggtt ctttaacttc
    17581 tcctttatct gcatcaacag cattttccca agaagctgga acttcaactt taactaaagc
    17641 atttattcct gcatcaacag cagcatagtt catattaact attttttcac cttttttacc
    17701 ataagcttta actatagaat cttttaagta tttaacagca tcttcttctg gtattatttc
    17761 agctaattta aagaatgcag attgcattat catgtttatt ctgtttccaa gacctatttc
    17821 ttgtcctagt ttaacagcgt taactgtata gaattttata tcatttttag ctatatattg
    17881 tttcatatga gctggtaaat gttcttcaag tcctgcttgg tcccatatag tgttaagtac
    17941 aaatgtacca ccttttttaa gaccttttaa taaatcatat tgataaacat atgattgttt
    18001 atgacaggca atataatcag cttcatctat taaataagta gctcttattg gagtatcacc
    18061 aaatcttaag tgagacatag ttataccacc agatttttta gagtcataat caaagtaagc
    18121 ttgagcatat ttttcagtat ggtcaccgat tattttgata gcttgtttat tagctccaac
    18181 agtaccgtca gaacctaatc cccagaactt acatcttata gttcctttag aagctatttt
    18241 tattggctca gatggagtta aagaagtatt agttacatca tcaactatac ctactgtaaa
    18301 tccattttta ggttgttctg aaactaagtt atcaaaaaca gtttttatat ctgaaggagt
    18361 agtatctttt gaacctaatc cgtatcttcc acctattatc ataggagcat tttcttttcc
    18421 atagaataca tcacgtacat ctaagtataa tggttcacca gttgaacctg gctctttagt
    18481 tctatcaagt acacatattc tttcaacagt agatggcata gcatctaaga aatgtttcat
    18541 tgagaatggt ctatataaat gaactttaac taaaccatat ttttctccct tagcatttaa
    18601 gtaatctata gtttcttcta ttgtttctgt tactgaaccc atagctatta atacgtattt
    18661 agcatcttct gctccatagt aatcaaataa actatgtttt ctacctgtta aattactcat
    18721 tttttccatg tatttttcaa ctatacctac tatattttga tagtatttat tagaagcttc
    18781 tctagtttgg aaatatatat ctgggttttg agcagtacca cgagcaactg gatgatttgg
    18841 agataaagca ttatctctaa atgcttgaac tgcttcaaag tttaataaac ttgcataatc
    18901 ttcattttct agtaattcaa ctttttggat ttcatgtgaa gttctgaatc catcaaagaa
    18961 atgtataaat ggtaatctac cttctattgc agctaaatgt gcaacaggtg ctatatcagc
    19021 tacttcttga actgaaccag aagctaataa tacacatcca gtttgtctag cagccataac
    19081 gtcttggtga tccccaaata ttgataacgc ttgagatgct aaagcacgag cacttacatg
    19141 gaatactcct ggtaataact ctcctgcaac tttatacatg ttaggtatca ttaataataa
    19201 accttgagat gctgtaaaag tagaagttaa agctcctcct tgtaaagaac cgtggaatgt
    19261 tcctgatgca cctgcttctg actgcatttc aacaacatta actgtttgtc caaatatatt
    19321 ttttcttcct tgtgatgccc actcatcaac tacttcagcc atagttgaag atggtgtgat
    19381 tggatatata gctgctacat ctgtaaacgc ataggcaaca tgagctgcag ctgtatttcc
    19441 atcaagtgtt ttcataaact tagccataat atgtatcctc cttgatagct acaattgtac
    19501 ttgtttataa caagtctatt tttttatatt tacatataat ttacacaaca aaaatataaa
    19561 cacacatata gtcgcgcaaa tactctatct tctaatataa tactacagat tagataaaat
    19621 tcctttgaaa attgtaaatt aattacatat ttaagtagat tttttttaaa attaaatatg
    19681 ttaagt
//
LOCUS       DAELNY010000060        19558 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.060, whole genome shotgun sequence.
ACCESSION   DAELNY010000060 DAELNY010000000
VERSION     DAELNY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.060"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   37..787
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=37..65
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1618..2490)
                     /locus_tag="KPE55_001829"
     CDS             complement(1618..2490)
                     /locus_tag="KPE55_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0492914.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(2884..3462)
                     /locus_tag="KPE55_001830"
     CDS             complement(2884..3462)
                     /locus_tag="KPE55_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0492915.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3520..4236)
                     /locus_tag="KPE55_001831"
     CDS             complement(3520..4236)
                     /locus_tag="KPE55_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0492916.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            4427..5512
                     /locus_tag="KPE55_001832"
     CDS             4427..5512
                     /locus_tag="KPE55_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0492917.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(5667..6326)
                     /locus_tag="KPE55_001833"
     CDS             complement(5667..6326)
                     /locus_tag="KPE55_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0492918.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6543..7835)
                     /gene="lysA"
                     /locus_tag="KPE55_001834"
     CDS             complement(6543..7835)
                     /gene="lysA"
                     /locus_tag="KPE55_001834"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG0492919.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(7853..9046)
                     /locus_tag="KPE55_001835"
     CDS             complement(7853..9046)
                     /locus_tag="KPE55_001835"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0492920.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(9407..10024)
                     /locus_tag="KPE55_001836"
     CDS             complement(9407..10024)
                     /locus_tag="KPE55_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492921.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(10194..11894)
                     /locus_tag="KPE55_001837"
     CDS             complement(10194..11894)
                     /locus_tag="KPE55_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492922.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(12291..12854)
                     /locus_tag="KPE55_001838"
     CDS             complement(12291..12854)
                     /locus_tag="KPE55_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492923.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(13013..13726)
                     /locus_tag="KPE55_001839"
     CDS             complement(13013..13726)
                     /locus_tag="KPE55_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG0492924.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            14018..15682
                     /locus_tag="KPE55_001840"
     CDS             14018..15682
                     /locus_tag="KPE55_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG0492925.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(15789..16688)
                     /locus_tag="KPE55_001841"
     CDS             complement(15789..16688)
                     /locus_tag="KPE55_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG0492926.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(16715..17353)
                     /locus_tag="KPE55_001842"
     CDS             complement(16715..17353)
                     /locus_tag="KPE55_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0492927.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(17694..18692)
                     /locus_tag="KPE55_001843"
     CDS             complement(17694..18692)
                     /locus_tag="KPE55_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG0492928.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(18790..19365)
                     /locus_tag="KPE55_001844"
     CDS             complement(18790..19365)
                     /locus_tag="KPE55_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492929.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         6405 a         3248 c         2042 g         7863 t
ORIGIN      
        1 agtagaaaga aataaacttt ttttagggaa taatttattt acattccata tagttaatat
       61 aaaacgattt gtaacattaa aaaagaataa aactttctca gatttacatt ccatatagtt
      121 aatataaaac gaataaccaa cccctaattt tttagcaatt tgttcctatt tacattccat
      181 atagttaata taaaacgatg taataaatga gtattcagtg catttcaaaa attatttaca
      241 ttccatatag ttaatataaa acattgtatg atttattttt atagcggtac tcctgggaga
      301 tttacattcc atatagttaa tataaaactt attccaagaa ggacgagttt tagcattcca
      361 agaacgattt acattccata tagttaatat aaaacggaga ttctggtgtt attaatattc
      421 agattcctta tcatttacat tccatatagt taatataaaa cttagatttt tagagtgcaa
      481 catgaaaaaa ggtctttaat ttacattcca tatagttaat ataaaactat ttagacaagt
      541 aaaaaaccac tctgcgcaaa agagatttac attccatata gttaatataa aaccctacta
      601 aacacgcttt agagggaaac aagttagatt tacattccat atagttaata taaaactaaa
      661 gttactctta ataatataga tagtcttgat gtatttacat tccatatagt taatataaaa
      721 cgctgatgaa aaagagcgtc aacaacttca aaaagaaaat ttacattcca tatagttaat
      781 ataaaacccc aaaataaatt aagtatttcc aatacttata catatacaat tctcctaaac
      841 ttgcagtgag cgagcaatag tgcaattgat aacagttatc atacactctc aacgctttat
      901 atttcaacta ttaaacccta ctttatcaca aaaatcgctc actgcaaaac ttttatattt
      961 ttattatatc ataaaactca tactataaaa taaaaatggg ataattttac aaactaaaag
     1021 catctacatt cttataagta tttattatat tctctattcc aatgattcct ttttcttttt
     1081 attgcatcct tcctatgtaa tcgcacctta gctcaataag tcttttattt tcattaacaa
     1141 ttttttaaat tattcaaaat catcaaattt ttcatcttat acagttaaat caaaatacgt
     1201 gttatatctc tttatctata tacttttctt ccttccattt cttttcctct ttatacattt
     1261 tcactttgtt actttacaat ctcaaggtta ttatctgcta ttaaagtttt atctttccag
     1321 gtttcacaaa aataaattga ataagtgcaa aaataacaga tacaaaacca taactatttt
     1381 cttttctata aaaaaatata taatcaacaa gaaatataat aaatgaaaat aactatgaat
     1441 aaatactctc atttagaaga aatagacaaa gatattcctt aaaatttaag caaaatattg
     1501 tttaaataaa aaaactgact gtcagttaaa tgccagtcag ctattttata tattttatcc
     1561 aaacatcact atatttgaac attttaataa ttaataatac ttattaatat acaaacttta
     1621 ctcatacaca gaatatcttt tccaaatttg ctctaaatca tcaaaatgct gtttaatatc
     1681 ttctttttcc ttcatcccaa cagatatagc aagggcattt gaaatactaa gagctggaac
     1741 tagagaatct acaaatgaag ccatattact cttaactaga agagtgttat ctgcaagtga
     1801 tgctactggc gcaaataagc tatctgttaa tgatattaca tgggctcctt tttccttagc
     1861 ataattgaca atttgataag atttcttaga ataacgaggg aaactaatag caactattac
     1921 atcttcttca tttattctta caatctgctc aaaagcatct cccatatcca ttctaattat
     1981 atgaacattg tcaagtatta tatctaagta aaaacctaaa tattgagcta ctacaaaaga
     2041 actcctcatt ccaagtatat atatttttct cgctcttaaa agtttgttag aagcttcttt
     2101 aaacgctctc tcgtcaattt cttctagtgt agaccttata ttatctatat cactttttaa
     2161 aactttgttt agtattgcaa aatcatcgga ataatcatgt gccatttcaa ctctttgtac
     2221 tgtagttagt ttatttttta tgagttcttg aagtgcagct tgtaatttag gatatccact
     2281 ataacctaat gcatttgcaa atcttacaac tgtagactca cttactccta cagtttcacc
     2341 caatttacat gcagtcataa atgcaacttt atcataatta tttaaaatgt attgtgctat
     2401 aagtttttga cccttactca atctcgtata ttgagattgt atatttgata tcaaatgttt
     2461 actgtccttc atttcatttg tttcttccat ttttttctcc ttttagttat ataaactatt
     2521 ttgttttatt aacactgtct ttttctctaa aaattttatt ttttatctca atttcatctt
     2581 agtttttatc atagcttctt aaactcataa aattctaact tttctgtacc ttatatttca
     2641 catagtattt tcgattaaat ttattatacc atctttcctt gcatttttta atatttatcc
     2701 aatatttctt ttaaattttt caatttttac tcatttaaaa gttatctatc ttattttgca
     2761 tatttagatg tatattttgt tttgagaata agctgttgta tacttattat ttaaagtatt
     2821 tttaataggt cacactatat atattaactc ttaatacaat cttgtcaagt tttttagtat
     2881 ttattatgct tttttctttt caattaaaat caattgtggt gggttattga cttgatttaa
     2941 aaacttagat tctaaaacat taaattcatc ttgatttaaa ttattgacaa ggttatatat
     3001 gcaatctttt tcttcttgac ctccatcatg ccctgtatat atggcaatgc ttattactcc
     3061 attttctttt aacatttcta aacttaactt tatagccttt aaagttgtat ctggtttggt
     3121 tataattgaa tgtttcgctc ttggaagata acccaaatta aataaaacac atgaaacttc
     3181 ttcttttata tattgattca ttttttcatg cccatcaaat atcaatttta ctctatctgt
     3241 atatccttct ttctctagtt tctttcttgt agactttatt gcttcttctt gtatatcaaa
     3301 agcatataca aagccctttt ctccaacctt ctcagctaga taccttgtat catacccatt
     3361 tcccatagta gcatcaatta ctatgtcacc ttcatttata atattttcta aataaaattt
     3421 atttatatct gtaattttag ttaaatactt tgcatatgcc ataatactat ttctccctac
     3481 tattaacttt atttcatttt ttctcaattt atttttatct tattttattg attttaaata
     3541 ttttatttca tcttctgtta ggtatctata ttctccaact ttggtgtctt ttaatactat
     3601 tttccccaca gaaatccttc ttaaatttaa aactggatga tttatagctt tgcacatttt
     3661 tcttacttgt ctatttctac cctcatgtat agttatttca cacactgaat aatttttttc
     3721 tttattttct ttagttaccc ttattttagc aggtgccgtt gtgtaatctt ctatttttag
     3781 tcctgtttcg aattgtctta tctcatcatc acttataatt cccttcacac ttgccacata
     3841 cgttttatca atttcatgtt ttggatgggt aagcttatat gttaaatcgc catcattagt
     3901 aagtattaat aatccactag tttcataatc taatctccct attggatata ctctttccct
     3961 tacatcttta agtatgtcta ttacactggg tctattgaat tggtctttta ctgtagttat
     4021 atatccctct ggtttattta atactatata tatataattt tcacttggtt ttactttttt
     4081 attatcaaat tctactatgt ctttttcttc atcaatatta aaagataact cttttactac
     4141 acttccattt acctttattc ttcctccaag aattatttct tctgcttttc ttcttgatgc
     4201 tattccacaa gaagctatat acttattaat tctcatactt aatccttcct tatcttaaat
     4261 tatgtgtgtt taatattttt atcaatgtta attttgctac attattatac tatactagac
     4321 cttaattaaa aacaagcaaa aaaattattt aattttccta aataattgta atttcttttc
     4381 acatactatt aatatgtaat agctgactaa acactatttt aaggaagtga ttattatttc
     4441 aaaggaaaat ataaaatatt atttaatagt cgtgtttatt ggaattttat tttttaaatt
     4501 tattaatact ccatcagact ttatatctag tattgaagga ttcttaaagt tttttagccc
     4561 atttttatta gctattttac ttgctttact tttaaatcca cttgtgatgc tttttgaggt
     4621 aaaattcaaa gcacatagac ttttagccat ttttttatct tatatattta taggttttat
     4681 tttagccttt gccattaggt tattaattcc atcaatagct aatacattaa atagattgat
     4741 aaatgaaatg cctatgtata ctgattatat agatagtttt atagaaaaaa atatgtcaaa
     4801 catagatttt ctaaaaactt taattcctca tatacaacat agtttagata atttattaaa
     4861 agaagcaagt aattttgttg gtaaaattcc taaaaatttc ttaatctata cacttagtat
     4921 atcttctatg ctatttaata tgacaatggg atttatacta tctatataca ttatatatga
     4981 taaagaaaaa attgcattgg gatttaagag atttctatat tcttctactg ctagaaacaa
     5041 ggcagataat attatagaat tttttaggac tactcatgat attttctatg attatttact
     5101 tggtagaata ttagactctc ttattattgg tataattgca tttttaggat ttcaatttgt
     5161 tatcagaata gaaaatgctt tatttttagc ttcaataata ttcttaggta atataattcc
     5221 ctattttggt ccttttattg gtgcaatccc tccaatagcc atgactatat tatatagtcc
     5281 acaaaaaact atatgggtca ttgcatttct atttatattg caacaattag atggaaattt
     5341 tatagaacca aaagttatgg gtaatcaagt tggtatagga gctatatggg taatatctgc
     5401 tattttggtg ggacaatctc tatttggttt cataggagtt tttctttcag taccaatagc
     5461 tgctgttgta aaaacttatg ttgataaata tatagataat cgtttacaat agcttatctc
     5521 tactgtgact ttaattttta gtttaaaatt taactgttat ttattaaatt atttctcaaa
     5581 aaatatctct tagtaagaaa aaattgctaa gagatatttt ttataatata taatttgttc
     5641 attaaattta tgcatatatt ttatacttat tgattatttg agttttgaga atttccacca
     5701 ctatctgaac caccagtagt atcttgtgga gttggtgctg gtgttggatt actattattt
     5761 ggcttttctt gattttgaga agaactgtta ttattgtcag acttattact atctgatttt
     5821 ttatcattat ttttagaact agatttatta tttttactac ttccactata agaagatttc
     5881 cttgcatctg attcaactct atacctatct tctccagccg tatcttttgc ctttttcttt
     5941 ctttgttcct ctgcttcgtt ttgttctttt tctaatttag ttaactcttc tttcttttta
     6001 gttgcctctt ctttaataac agaggaacca tttttgattt tctctataag tttaagttcc
     6061 ttgattgcac cctcataatt ttttaattct tcaaactccg tcatatttga cattctcata
     6121 gcttgcatat acatagctct tgcattctca ttagtactat cttcttccag tgcagaggat
     6181 aatgattcca tagctttttt atagttatgt ttttctaaat atgtttttcc ttgctctact
     6241 aaatttattt catctttttt attaaatgaa catcctacaa gaaatataga taatatcgat
     6301 attaataaaa ttactcttcc tctcattaaa tccctccttg tcatatactt taaattttat
     6361 cataattata aagtttttaa aatattttat atttattaca agaaatttgt tagtaaaaca
     6421 aaaaataagc agatatgcta cgaacgatac aataaaccaa aaattaattt agagatacat
     6481 atcctagcaa atctgctcat aataaacttt aatatttaat ttttattata tacttctcca
     6541 ttttatatct ctaaagctaa taagtcctca tagctttgtc tcttacaaat tatcttatca
     6601 tttccatcaa atactgatac tactgctgct cttggtattt tattataatt tgaagacatt
     6661 gaatatccat atgcaccagt tgatgttgtt ataagtatat ctccactttt aatatccatt
     6721 atgtttatat ctccaattaa tatatctcca ctctcacaac attttccagc tattgttaca
     6781 tgattcatag cctcatgatt aattttattc acaatactac actcataact agcttggtac
     6841 aacgaaggtc ttacattatc tgtcatacct ccatctacac ttacataaac cctaacatct
     6901 tttatatctt ttatagaacc tactgtatac aatgtacttc ctgcatttgc aactattgat
     6961 cttcctggtt ctattaccaa tgtgggtact tctattccaa gctctttaca tgaagtttct
     7021 gctttagtga ttatagtttc acaaaactct tttatagttt taggtttatc tccttcgcta
     7081 taataaacgc caactcctcc acctaggtca atttctgtta gttgtatgtt gaatttttct
     7141 tttatttctt ttaccaagtt catcattata tccactgtat ctatgtatgg ttctacatca
     7201 aatatttgtg aacctatatg agcgtgaaga cccacaagtt taatattttt atattcactt
     7261 aatttttcta ttgctctaaa taaatctcca tttactaaag caaatccaaa tttagaatct
     7321 atctgtccag tttttatata atcatgtgta tgagcttcta taccaggagt tattctaaag
     7381 tatatctctt gagttttacc tttatcttca cataatgctt caagcaaatc taattcataa
     7441 aaattatcca ccacaaattt acctacacct aaatctaatc ccatgtttat ttcttcttta
     7501 gtcttattgt ttccatgaaa gaaaactttt tccataggaa aatttgattt ataagctgta
     7561 tataactcgc ctcctgaaac tacatctaaa cacatttgtt cttcatttat aagattacac
     7621 atgtaaagag gtaaaaaagc tttccctgca taagcaacct tattttttcc ctctctagcc
     7681 ttaaaatatt ctacatattc tctacaattt tgtctaacta aagcttcatc aaatacataa
     7741 agtggagttt gatatttttt actaagttct aaacaactca ctcctccaat atataattca
     7801 tcattattta cagtcatagt tccatgaagt ttcataatag cctccccaaa ttttatatat
     7861 taaatacttc tgctattttt tctatagcta cttgcttatc atcagaattt atagcacaag
     7921 ttatacgaat ctctgatgta gttataagtt ttatcattat accattttca ttgaatatct
     7981 taaatacttt agcagcaact ccagaagtat ttttcatacc aagacctact aaagaaaatt
     8041 tagtgatatt attatcaatc actacatgtt cttcatctgt atatttagat actatctttt
     8101 tacattctcc caactcttct tttggaactg taaatgatac acttatctta tctaaaatag
     8161 gtgcagtttg gcttatcata tctacactta tacctatagt tgctatatct tcaaataagc
     8221 tagatatatt ttcagcttta aaatctttta ttgttataga actgtcatca tcactagtag
     8281 ctaatcctgt tattacttta tcctctaatt tcatgtcttt tccccctctt atatatgttc
     8341 ctttttctgt accacatgta cgtccgacat agatttcaac accatatttt tgtgcaagct
     8401 ctatacttct tgaatgcatt acttgtgctc ctaaactcgc taactcaagc atttcttcat
     8461 attcaatctc atcaagttta ctagcctttg aatattttct tgggtctgta aaataaattc
     8521 catcaacatc tgtatatatc tcacattttc catttaattt aaccgcaaga gctacagcag
     8581 atgtatctga accacctctt ccaagagtgg ttacatcacc atcttcgttg attccctgaa
     8641 atccagttac tatcacgaca tttccttcat ctaaactatt tttaattctg tttacgttta
     8701 tatcatcaat ttgagacttt ccatgaagac cactcgtttt tatatttaac tggtaagcat
     8761 tataacttat agctttacat ccaagtgcat ttaatgctat tgataataaa gatgctgaaa
     8821 tcatttcacc agtagacatt aaagcatcta attccctctt actaggttca ttactcaatt
     8881 cctttgctaa agttatatat tcatcagtgg attttcccat ggcagacaca actattacca
     8941 tttgtggatt ttcttttctt cgttctatta tattttctgc tattgatttt attttttcag
     9001 tatctgctac tgaacttcca ccatattttt gaacaattat actcatgctt tctccccctc
     9061 aagctaaatt ttgataaata aaaacgccca aaggtatact ttggacgtgt aaatttcata
     9121 gaccaagtaa ccttgtagct caccacttta aaagtgacag ccttatgcat attctgcata
     9181 agtccagaaa atttgtccgt caacaaaatt tcttcggcta tatacccttt catcaattat
     9241 caacgcttac cagctcctat tgaatactat tgaatacagc tacctctacc ctaggctcat
     9301 tatttttaat taatttatta atatagtttt atcatattct atataatatt gcaacaatta
     9361 ttttacaaaa tgtggcaaat cgatagtttt cttttctaaa gttagcttat atacaattaa
     9421 atagtttatt taatcctaaa tctataaaat ttatgctata ctcattatca tatctctcta
     9481 aatcatttaa ttcggataga ttatctggtg taactaccaa aacgtttggt actttaaaaa
     9541 atggaattgt agatttatct atttttttag aaagattttc gtatttagaa atgtctattt
     9601 cattatttaa gtcaacatca actaaaactt gctttatatt accaaattgg tctgtatatt
     9661 caataaataa atcgtatttc acatcatcta cactaacatt tcggtaaaat ctttttacat
     9721 cacaacctgc agtattgatt ctaacaacta attctgatcc tactagagcc tttattaaat
     9781 catctccctt aaaaagttta ctaccgttta aatagtatac ataagagagg tctttataat
     9841 tataataatc cacatcacaa ggatattttt ttagtgtata ttcactacac atttttctca
     9901 aagttttttt aaaatttttt ttattgtatc ttttttggtc tccataaatt tttctaaatt
     9961 gagactctgt aacacatcta accctactaa ttaaaagtaa tatctctttt tccatattcg
    10021 tcatagtaca ttctccttga agtattattt gcaaagttgt actaatatta tattaatttt
    10081 atgacattaa atttacaaag aattaaaata aaatagctaa tttttataga attaaattct
    10141 ataaaaatta gctatcacta tgttaaatac tattatcaat ctttttattt aaattataca
    10201 ttaccttgat tttcagaatc tttggaatct gtattttcat cagttcctgt tttttctttt
    10261 gcttttttat cttcaaagtt tgcaatattt aggttatcaa attctgacat ttctattaca
    10321 ccagattctt cttttgaata cgatattttg actttatcac caacctgagt caatggcaat
    10381 tcgctagata cttttgaagt agccctaaat attatatctt tatttgaatc taaagttaca
    10441 taataattag tgttaccacc tcgcacatct gggcttattc ttgtcactgt gccttctaat
    10501 acctcatctt ttaaatcatt atcaagtttt aaatcattac ccttagattc taatgcatcc
    10561 ttatagtttc ttaatgcttc attcttatct tcacctattc caagtacatt ataatcttca
    10621 acagatacaa atgcaactct cttaacaaga ccagccttat cttttaatga tgatacatat
    10681 gttggctgac ccaatatatt atacattaca gggaacgtcg cttcataatt tttttcttga
    10741 acattacctt cagctgattt cattgcagcc gtttcagtag ctcctggttg tttatataat
    10801 ctagcttctt ttgttctaga atcaactaac ataaatccaa ttgtagattc atctccacct
    10861 gaagatgtta tacctgtata ccaataagac ctgttatcat tgccataaac taatgatgta
    10921 ccttctgttg gtactaaaac tcccttttct gatataacag agtttaaaaa tccattaaca
    10981 taaacacccc agtctttaat ctggtctgtt acaaagcttt gtggttgtat tctatccacc
    11041 catttaggcg tgtttttaac atcataaaca tttatttttc cagtttctgc atcaacagtg
    11101 gctattcctg ttgcatttgc tcctccatag ccaattttat gttcatataa acttacaacc
    11161 caataaggtc ttccttcatc atttatttca agagtgaaat ctgtcatacc aacatttaca
    11221 ataccatgta tgtataaatg tctttgtaaa tcctgatgta agtaggcttc tggttggtaa
    11281 actatcttta ttggctttcc atctatttct tgaactaatt ggacgtcttg aggattacta
    11341 gctgatactt ttacataacc acttgtacca cttagagatg ttatccattt aattatgtct
    11401 ctgtgaacaa gaggcgctac ccagtacaac tctccatcta cattttgtat atgaaatttt
    11461 cctaatttgg atacactccc tattgcagga acttctccaa gctttttgtc acctaatttc
    11521 attgccatat cttcatctac aagacgaatg tcatttacac tgacagggct aatgtcatca
    11581 gtaaatttac tttcagttac ttttcccaat agttctctat atgattttgc atgaagtaca
    11641 ggtgtagaag ttataaaggg aactactaca atatacacaa gcaaagctat tgctattgaa
    11701 aaattatatt tagccatttt agatgctctt tcatttctat ctaacatcat atctagtatt
    11761 cctgctacaa caaagaaaat tattcctact gaaaataaag atgtaaaatc taatcttagt
    11821 acaggtaatt taataaatgc ataaattatt acaattatta atgcaactcc atatgtttta
    11881 agatatcgtt tcatttaata ctcctctcta aaaaatgtta ttattactat tatatccata
    11941 tatttacaaa aaataaatat ttatcatatt tattatatat taagatatct ttatatattt
    12001 atagttctgc ttattcaact atatttaaca ttatacgtgt atgtataaat actttttata
    12061 cactgtttgt actatccatc ccgcacctaa aggagtggga tttttgcact attctgtatt
    12121 atatatatcc aaaaatatta taaaaataca aatattagca gaaatttaaa aactgaagaa
    12181 agtattctaa ctaagattta caaattctta atcctatcga agttaaacta ctatcttcag
    12241 cctaattatc tagcaatatt tttttatatg aaaaactcta aatcaagttt ttaaatgttt
    12301 gcgatttttg atacataaga atgttgtcta gaattatatt ctgataataa accttcatac
    12361 tctaaggtta agccagataa attataaaaa tcatttggcg ttacttcaaa tgcttgttta
    12421 tttaaaatgt ctatctcact tttttctaac atgtcagcaa caaaatttga acatacatat
    12481 ttatattcag attgtctctg aatattcatt agtaaatata ttaatgccat tgtatcgtaa
    12541 tagtaatctt ctctataatc actaattaat tttatatttt tatatagatt ttcatattgt
    12601 aaattatcta cttctaacga atatacccta cacacagtgt tttttcttat agcatataat
    12661 ccttgattta tattttcttc aacaaatcct ccaataaatg gattccttgg atttagcctt
    12721 ccaaaagaat acattggttt tagattttca tttaatgata ttgacacatg tgtatacagc
    12781 tttttactta tattccttat taagtaagat aatatagttc ctgtatatgt tgttacaata
    12841 tagactttat tcatcatttc cccccaatct agtaactaaa taatattata cacttacaat
    12901 ttaatatcat aagatattct aacataataa atgtggtttt acaaattttg attttaaggt
    12961 actttaatat tatataatat aataaaaata taaatataag ttttctatat ttttaaccaa
    13021 cagatgtttt cttcaaatta atcagatgat ttagtatatc ttgaaccttt ccagagtcct
    13081 ttaaatatcc taatgcccca tttatttgtg attttaaatc tatttctact tccatatcca
    13141 aatcaacatt tccttttttt atagatatat ttttaagtgt tattggataa agataacttt
    13201 tttccaaagt aatcttattt ccattaacct caaatggaac ttttttattg tagttactta
    13261 atattttttc tagcatttta tcacttagtt taaattttcc aattttcaca ttctcaagac
    13321 ttacaactaa gtttccatcc tttaaagtag gtctcaattc aagttcatat tgactattta
    13381 ccaaaccaaa taccttatat ggtcctgcta cctttatttt accatttttc atttcaacaa
    13441 aattattttc aagttcatta attccatgct tctgcatcaa tgtatatatt aaatttttaa
    13501 actcttcctc tgaaacagat atttttccaa ccattctaag tggatttttt actacctcca
    13561 tagaatttat atatgagcct tttgataaat attcttcatc tatgatttta ttattttgtg
    13621 aactaacatc atatctttct tttggtgtca agatatatac aacaattcct acaagtacta
    13681 aaacaataac aattaaactt aacaaaatct ttgcaacctt tcccataatc gtctccttta
    13741 caaatctgtc atcagattta aaataaattt agataactaa cttcacataa tattcttaac
    13801 aatatacata ctataataca gtcttttaaa aattaattca accaatctaa tgttttgtaa
    13861 ataaactgta aaaacatgtt tttagtataa aagttaatat tttgcataat actcttaatt
    13921 acacctttat ttttttctaa tcttggatta aaattaatat tatgcaatac agtgtagtgt
    13981 tctgtattat aatcaaatta aataggaggt atagaatatg tcaaatgaaa cgaactcatc
    14041 taactttata aagaatatta ttataaacga cttagaaaca ggaaaacatg acagtatcat
    14101 aactcgtttt ccacctgagc caaacggata tttacatatt ggtcatgcaa aaagtatatg
    14161 tcttaatttt ggattagcta aggagtttaa tggaaaagct aatttaagat ttgatgatac
    14221 aaacccatta aaagaggatg ttgaatatgt agagtccata aaagaagatg taaagtggtt
    14281 aggatttgat tggaacgagt taaattttgc atctaactac tttgatgaaa tgtataagag
    14341 agctttaata ctaataaaaa agggtaaagc ttatgtatgt gatttgacac aagaggaaat
    14401 gagagaatat cgtggtactc taactgaacc aggaaaagaa agtcctcata gaaacagaac
    14461 tattgaagaa aaccttgatt tatttgaaag aatgaaaaat ggtgaattta aagatggtga
    14521 aaaaacatta agagccaaaa tagatatgtc ttctccaaat ataaacctta gagacccaat
    14581 tatatataga atatctcatt caacacacca taatacagga gataaatggt gcatatatcc
    14641 tatgtatgct tttgctcatc caattgaaga tgcaattgaa ggaataactc actctatatg
    14701 tactttagag tttgaagacc aaagacctct ttatgattgg tttgttaaag aatgtgagat
    14761 ggaaaatata cctagacaaa tagaatttgc tagacttaat ataaataata ctgtcatgag
    14821 taaaaggaaa ttaaaacaac ttgtagatga gggtattgtt gatggttggg atgaccctcg
    14881 tgttcctaca atatccggaa tcagacgtaa gggatatact gcagaagcat tgcgcaattt
    14941 ctgtagtgaa ataggggtat caaaagttaa ctctacggta gatagtcaaa tgcttgatta
    15001 tttcctaaga gaaaacttac aaccaaaggc tcctcttact atgggagttt taagaccttt
    15061 aaaacttatt ataactaatt acccagaaga taagattgag atgcttgaaa tagagaataa
    15121 tgctaaagat gaatctcaag gaaagagatt agttcctttc tctagagaat tgtatataga
    15181 acaagatgat tttatggaag aacctgttaa gaaatatttt agatttttcc caggaaatga
    15241 agtccgttta aaaggtgctt attttgtaaa atgtactgat gtaattaagg atgaaaatgg
    15301 aaatgtagtt gaaatacatg gtacttatga ccctgaaact aagagtggct ctggctttac
    15361 aggacgtaag gtaaaatcta caatacattg ggttgatgct aaatcagcta tcccatgtga
    15421 atttagatta tttgaaccat taattcttga tgatatacct gaaaatgaag gtaaacattt
    15481 cttagaacaa ataaatccta attcattgga aatattacaa ggttttgttg aacctacaca
    15541 gataaaagat gctaaacctt ttgataaatt ccaatttgtt agaaatggat tctttagtat
    15601 agataataaa tatacaactg atgaaaaatt tgtatttaat agaatagttc ctctaaaaag
    15661 ttcttttaaa ccaggtaaat aatcttacaa tcaaaaaatg aagacttaaa atataaatta
    15721 cttctactat aaataaactt agttagaagt aattttttat ttgagtcttc atcatttact
    15781 ttaaatgatt aacttttttc actacagatt cccatgactt ctattccatc ttttatgcat
    15841 tctatgttaa gaggaaaatc tggtcctttt tctccgtcaa tatctgttat aagtgaatct
    15901 tcacattcta tatgcacctt tttagtttta aagtatagta tttcatttcc attatattga
    15961 tctaaataat cacctctaag cacacttatc aaaactggta ttgattttgg tattggcatt
    16021 cctttaaaaa taattacatc caaatatcca tcatctattt cagccttata tgctagattt
    16081 atatttcctg ctgttttgcc attaaaaatc aacataagat acatatctcc atcataatac
    16141 atttcctcag aagatacttt tatcttaaat ttcctcatat acaatgcttc ttctattccc
    16201 tttatataat aagaaattct tccaaatgaa ttttttaagt ctgtgtttat cctttgtgat
    16261 acatccgtaa acataccagc acttgcaaca tttataaagt acttattatt gactttccct
    16321 atgtctattt tctttggttt tgaatttatg atttcattta tagcttcttt tatatcaaat
    16381 gaaatttgaa gagcatttga aaaatcattt gcagttcctg taggcaaaat tccaattgga
    16441 atttttatgt ctaattcttt cattgcattt actactaaat ctacagtacc atcaccacca
    16501 gctatcaata tgtgttcata agaactatca acttccttaa atgcattctt tacaggttct
    16561 ttaatagtta atctataggg tatcaataaa taattttttt cttgatataa ttctatgata
    16621 gtgtctaaat ttgataatat ctttttttca cctgaattag gattgtatat aagttttact
    16681 tttttcatta tttctcctta ctcattttat tcttttagta tccaaaatct tcatttaaaa
    16741 aaatcacatg tattctttct ggtgttaatt gtctttttat aagtgtataa ctacagcata
    16801 ttcttcctgg acttgaacag tctatacata taccagtagt cttacatcca gtatttgtac
    16861 ttaatctttt acaattggct ggagcagcta ttgtttttgt tctttttata gcttcatcta
    16921 catctttaac tattttattt actcctgcta taactattac tttatcaggt ccatatatca
    16981 tagcagcaac cctattccca ttcccatcta aattgtaaag ttctccatcc tctgttatcg
    17041 cattagtact tgtaaaataa gcatctgcag aaaatgactt tctatatatt tctttcattt
    17101 cttctggttt gatatctttt gcatatctat caagaaaatt atatttttct cttataaaat
    17161 caatcactcc tgtttcaaat aaggtcatag aacctccaca tcctactaga gaacctgctt
    17221 taactatttc ttcaattttt ttaagaagct cttctttgtt acttactaaa taccctttca
    17281 tattattgtt ttccaaagat tttattgttt tttctattct tttttcatta agccaattta
    17341 aattttgatc cattttaact tcccccttat ttttaatatg tacttaatta tttataaact
    17401 ttattttctt tataagttta ttttagtaca tctaaaaaaa aatacaatat aaaacttaaa
    17461 tagttttaca taattttttc caaaataact tactcaatca aaaaaggaaa gtagatgtac
    17521 tattctactt tccctatatt gtatgtagta ttatatattg cctaaaatct atatattaat
    17581 tatatgtttc atattatatg aatatcacag ttaattaaaa tgtaatacta tcttcttttt
    17641 aaacatataa attgtattat taaatttaat atatccaact tcaataaatc ttattaattt
    17701 gatgaagaag atgctatagc tgcacttgtt gctgccatta cacatgcaat ttgaatagca
    17761 acaacaatat ttaaaaatat attattagtt atagagccta tattattctc atttcgtatc
    17821 aaatctgcat aaattgaagc tacaatagca gaactaatca ttactctatt actttttcct
    17881 aaagaccaat ttccaaagcc tttttcttct ttaagtttat ttgaaacatt ttttatctct
    17941 tctattattt catcttctct ataatctaat aatgaaattg ctcctattat tggatatcca
    18001 taataattca ttttacaatc attttcttta aatgcttttt taagttcaat agtcttttta
    18061 catttgagca tagttctatc atcactaaat aaaagaattt gggataatgc ttgtaaatca
    18121 tttcctttat aaaaaccatt ttcatttaga aattcataac atttttctat gtattctaaa
    18181 tcttcatcta aattttttga atgaattgca ataagtgcta tattacaata gtcatcacta
    18241 gaagttagaa atggatgttt tttcctcatg taatcatata ttattttcat ttttgatata
    18301 catgtaagat aatccatttt ctctctattt tcataaacta ttgatgctgt aagtggtaaa
    18361 tatgtatcgc cccaaaactt ttcactttta agttctttgt atatatctaa aatcatctta
    18421 aaactacttt ctggatttga ttcaaacgaa agtagtgttg ccatataaaa catattatat
    18481 cctctaaaat tagagaataa tccagtattt tttttaataa gctctattcg ttcttccact
    18541 aatctaggac ttattctctt gttcttaagg gtaaatgcaa gtgcacaact atgttgtatc
    18601 aatcccattt gccaagaacc tttaacttca tttaatgctt tgtaattttc tattaataag
    18661 tttacttttc catttataaa cgattgttcc atattgccct cctagtaata tatctcattg
    18721 tactaatagt tttatattta ttatatatat attaaatact acaatcaata catattactt
    18781 caaagaacat cattcttcaa caataatgta gccttttttg taatgtcttc ccatattatt
    18841 gactctatca tagtactctg attcactttc attactccat ttgggcacaa atacatctat
    18901 tgcattagaa ttactaaaat atttactaga ccccctatcc ataacagttt ttaattttcc
    18961 atctacaata atcttagttc caaaacttaa gtgattgttt gctacaaaac catcttctaa
    19021 atcatctcct gtagcagtta tcgctccata tccattttct tgattaagac ttgtgtaata
    19081 actaacttca aaagttatag gtactaactt ctttatttta atacctaact gattttgtag
    19141 tttctccttt tgtactttca acttatactc atcactattt ttataatctt ccataccttg
    19201 attatacttg gaattaaagc ttttacctga ctttactata gtatctatat ttactataga
    19261 ttctttacaa tgatctgcat ctatttttgt ttctccattt accaataatg gagttaaaca
    19321 tacactacat acaacacatg taatcaatgc ttctttaagc ttcataaatt ccccccttat
    19381 tattttgata tattagcata tttaatatta tatgtctaca tttagattta aataccacac
    19441 ccatttgctt taagaagttg tcttatattt aattcacctt aaacacttat aaatcctcta
    19501 tcttagaatc atatagaata aagcttttat ctttaaatat tttttattaa tcttttaa
//
LOCUS       DAELNY010000061        19460 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.061, whole genome shotgun sequence.
ACCESSION   DAELNY010000061 DAELNY010000000
VERSION     DAELNY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.061"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(50..658)
                     /locus_tag="KPE55_001845"
     CDS             complement(50..658)
                     /locus_tag="KPE55_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG0492930.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(767..3505)
                     /locus_tag="KPE55_001846"
     CDS             complement(767..3505)
                     /locus_tag="KPE55_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0492931.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3653..4444)
                     /locus_tag="KPE55_001847"
     CDS             complement(3653..4444)
                     /locus_tag="KPE55_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0492932.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4480..5136)
                     /locus_tag="KPE55_001848"
     CDS             complement(4480..5136)
                     /locus_tag="KPE55_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492933.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5129..6094)
                     /locus_tag="KPE55_001849"
     CDS             complement(5129..6094)
                     /locus_tag="KPE55_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG0492934.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6200..6306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6596..7540)
                     /locus_tag="KPE55_001850"
     CDS             complement(6596..7540)
                     /locus_tag="KPE55_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0492935.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7651..8118
                     /locus_tag="KPE55_001851"
     CDS             7651..8118
                     /locus_tag="KPE55_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492936.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8288..8887)
                     /locus_tag="KPE55_001852"
     CDS             complement(8288..8887)
                     /locus_tag="KPE55_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492937.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9133..9729
                     /locus_tag="KPE55_001853"
     CDS             9133..9729
                     /locus_tag="KPE55_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG0492938.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9827..10786)
                     /locus_tag="KPE55_001854"
     CDS             complement(9827..10786)
                     /locus_tag="KPE55_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0492939.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10820..11551)
                     /locus_tag="KPE55_001855"
     CDS             complement(10820..11551)
                     /locus_tag="KPE55_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0492940.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11544..12314)
                     /locus_tag="KPE55_001856"
     CDS             complement(11544..12314)
                     /locus_tag="KPE55_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0492941.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(12399..13109)
                     /locus_tag="KPE55_001857"
     CDS             complement(12399..13109)
                     /locus_tag="KPE55_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0492942.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(13289..13441)
                     /locus_tag="KPE55_001858"
     CDS             complement(13289..13441)
                     /locus_tag="KPE55_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG0492943.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(13467..15623)
                     /gene="feoB"
                     /locus_tag="KPE55_001859"
     CDS             complement(13467..15623)
                     /gene="feoB"
                     /locus_tag="KPE55_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0492944.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(15723..15944)
                     /locus_tag="KPE55_001860"
     CDS             complement(15723..15944)
                     /locus_tag="KPE55_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0492945.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(15957..16169)
                     /locus_tag="KPE55_001861"
     CDS             complement(15957..16169)
                     /locus_tag="KPE55_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0492946.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(16490..19387)
                     /locus_tag="KPE55_001862"
     CDS             complement(16490..19387)
                     /locus_tag="KPE55_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0492947.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
BASE COUNT         6362 a         3373 c         1972 g         7753 t
ORIGIN      
        1 tacctataaa ataccttcct atagatacat atcttaccta tataaaaact tattctatat
       61 aaaaacgcca taaaaaatct tttgcttctt ctgcataatc tatatttata cgtttactag
      121 tttttatatc taactcatct ttactaaata ctttttttcc tttatcatca taataaaaat
      181 cacttatata taattcttca cccattatac tcttactatt tagacttcta tctattttta
      241 aagctttgca aagttttcct ggtccgttag taatattttt tacttgatat ttactcaact
      301 cagtgtaagc tttgttgtat ctattcatag atatctcatc taaagaagtt ataggctcta
      361 cccctcttat taatacacac tctggcacat tttctatatt tgcagataaa ttcaagcaat
      421 tatacatacc ataaattaaa taaacatata tatgtccacc atctaaatat aatggcttag
      481 ttctatctgt ctttttattg ccatatacat gtgctccttt atcatttaca cccatgtatg
      541 cttctgtctc tatgattttg gtaacaatta ctttattttc atatttcctt attagatatt
      601 tacctaatat tgatttggct aaatcaattc cattttttct aaaaaaatcc ttttccaaaa
      661 tttcacctca taaaatttat ttaaaacgca tcaaatacaa ctaaagtaat aaataaaatt
      721 aactcttatt aatgtgatat atataaacaa taaatttaga catttattat ttatatatat
      781 caaaaaattc tatctcaaac ttttcaatat tatcattgta atttgaatta tcatatactt
      841 cttcaacaac ctctttaatt gggaatataa cttttacatt agttccttct cctaccttac
      901 tatctatgta aatcttccca ttttgaattt caatcaatga ttttgtaagg cataaaccta
      961 taccacttcc aaattcattt gatataccac tgttttcaat ttgtttaaat cgattaaata
     1021 tatcctttaa tttttctttg tctatgccca ctccagtatc ttttataatt atctctactg
     1081 tttcgtcttt tttatatata cctacaagga tttctcctcc ttctaaagta aattttatag
     1141 catttgataa tatatttagt attattcttt ctatcgattc tgggtcaagc cctaccataa
     1201 cagtttcttc atctgtatca aaaattaaat ctattccttt agacttagca taactactaa
     1261 tagatgtaac tattctttct gttatgtcaa taatatcaaa attaactgct ttaatcttat
     1321 atatgtctga cttaacttga gaaatattta ttatattatc tattaatctc aataatctaa
     1381 acatattttg tttagttatt tttatatgtt cttttagtat atcctgaaaa tcaattattt
     1441 ttcctttttt ataaaggttt tcaataagtt gaactgaact atatatatta tttaaaggtg
     1501 tcttaagttc atgagatata ttagcataaa actccatctt catttggtca tactgtcttt
     1561 gttttattaa tgctttttta agtctttcag actctctttg cacacttaaa tctttaataa
     1621 ttctcactct acttcttttc tgtccaaagt ataatgttct tgatatcatt tttacttctt
     1681 taatgtgatt attttcattt actatatgga tactcttctc tctttctcct atatttaaat
     1741 tgtttatgta tttttttact aatatatttc tatatttttg tgttgtaact cctaatacat
     1801 cactagaaat tccgaacaac tctctaaagg ctctatttgt atatagtact cttgatgtac
     1861 ttaaatcctc aagaaaaata gcctctggta taacatccag taatttttca tatattattt
     1921 tttgttcatt tactgttcta agaatattta tctctttagt tatatctcta aatgtaagca
     1981 atttaaagag tttattatct gaccaatagt ctccaactct aacttcttca acatgttctt
     2041 caccttctga gtcagacatc acaaaattaa ctatacagtt tgctgtgtca ttgtaactta
     2101 ctatttcagc tttcttttct tgtgttagat ttaatctttt aaattcccta ttgtcaaaga
     2161 aatctatgtt aaaagtttca aaatattgtt ctaatgtcat atcgactaaa tcacttgaaa
     2221 cacatttaaa actcttttca tattttttat ttatacttat taccttattc ttctttatgc
     2281 caccttcagc acttcttaca agtataggag atggaatatt tagtataatg ttttcatagt
     2341 ctttatttat atttcttatt tcttcatcta ttttgatttc atttgtaata tcatttaaac
     2401 ttattaagta attgctttcg tctttattat tacttaaact taacaaataa attttatcat
     2461 ttaactctac cagtttatca attatactta tatcgttatc aaatttcttc aatttttcag
     2521 caactttttc atcttccttt ataaaagtta atattttata ataattattc atatcctttg
     2581 aaaattcatc tttaaattta ttatttgtgt aacttatatt aaaatcatag tctgttacta
     2641 tccatacatt tggtattgta tccagagctt gtctaagtaa ttcttttctc tggtctatgt
     2701 agttttttaa tgttctttgt ataatcatat ccttttctat tgctaaattt atctttccta
     2761 attgctctga ctttttactt aattttttat tgattgaatc aagttcataa tatggataca
     2821 ctactctatt attaagactg tatacatata cataataagt atggttacat aatagtaaat
     2881 atattactcc tattaatcta tctgaatcta ctatattaat cttagaaatt gctacaaaac
     2941 atataatagt tgtaaaaaaa acaaaatatg ttaaatcttt tattaactta tagtgctcaa
     3001 tgttactatc aagtttattc aacaaatata gtaaattaaa catagatata caaatattaa
     3061 ttaatataat aatcatattc cagttaatat cactaaaaat atttcttata aagtaatcta
     3121 atatcacaaa agcactataa aaaattagta ttgggcttat tcttagtttt tttactttag
     3181 aaatggttaa gtatataaaa gtagacatat taattatgct taagttaagc aatacaattt
     3241 ggaatttttg aatttttaaa tttactactc ctaaaatact tacaaaaact aatacaagac
     3301 accctacttt caatatattt ataaattcat ctgttttaaa gctacttata taaactatag
     3361 aatatccaaa tatccaaagt aaaattattc tatacatatt tatagaaact gtatacaaat
     3421 ttatatttaa aagtgcaata accaaaaata acccattagc tagtatatag accttattct
     3481 tacttaaagt tcctttagat tccatattcc ccaccacttc ttcattttta tatagtttta
     3541 cctataaata tgttaccaca attcttccaa aaaatttgtc actttctagt tataaaaaaa
     3601 aatgagtaaa tatttttaat tttactcatt ttgtagattt tgtatattca gtttaaaaag
     3661 ctggaactat acttccttta tattcatctt gtataaattt tttaacttct ttactattca
     3721 tagcctctga taaagcctta atcttatcac tatctttatt attttctcta caagctatta
     3781 tatttacata tggagaatca cttgattcta ttactattgc atccttagta ggatttaagt
     3841 tagctgttaa agcataatta gaatttatta ctgctccgtc aacatctttt aatactgttg
     3901 gtaattgctc tgcattcatt tcttttatta ctatattttt aggattttta gttatatctt
     3961 ttttagttat aagctctcca tcttttactt ctatcaattt attttttgct aataatttaa
     4021 gtgctctagc tccattagtt gcatcatttg gcacagctat aacagctcca tcttttaatt
     4081 catctaatga cttaactttt tctgaataga atcccattgg ctctatatga actttagatg
     4141 tataagtaag tttatatcct ttctccttca cagtttcctc taaaaatgga atatgttgaa
     4201 agaagttagc atccaaagac ccttcatcta aagctgtatt tggaagtaca taatcatcaa
     4261 atatttttac ttctaaatca tatccttttt cttttaacaa aggttttgca acttccaata
     4321 tctttgcatg tggatttgat gaagctccta ctagtatttt tttatcctct ttgtttgaac
     4381 aacctactgc tgatattgct attgctgaca ccaatgctac tgataataat ttctttaatt
     4441 tcatttttac tccccctctt taaaatgttt ataattaatt tattttagtt tcttataagc
     4501 cagatttcct actccctgga ttatctgaac taatattata agtgctatta cagtatatac
     4561 cattactgct gtatcaaatc tttgatatcc ataaataagt gctatattac ctaatcctcc
     4621 agctcctact gcacctgcca tagctgtata accaagtata gatattattg ataatgtaat
     4681 tccagatact attgaaggca tagcttcctt tatcataact ttaaatacta tttgtctttt
     4741 tgtagctcca aaagattttg ctgcttctat caatccttta tcaacttcat ttaatgagct
     4801 ttctataatt ctagcgataa acggtgctgc cccaatagtt attggcacta ttgcagcagt
     4861 ttcccctata cttgttccaa cgattaatct agttattgga atcattgcaa ctataagtat
     4921 cataaatggg aaacttctaa atatatttac tataaatcct aatgcactat ttattgtgct
     4981 atttggtttt aatccatctg gttttgtaac tactaatatg attgctaata taaatcctaa
     5041 tattgttgcc actatagttg gtactataac catatacaaa gtttgtaaca atgcatttgg
     5101 aaatagtgtt gttaaaaagt caattaaact attcatttat catttcctcc catcttatgc
     5161 ccttactagt tagatattgt tttacttgct ctccactttt atctgaaatg tttataataa
     5221 gtgaacctaa aatatcatct ttaaattgtt ctaatttccc aaatattata gacacatcta
     5281 tatttagttc tcttgccata gtcgtaataa tagcttcatt agaaatatct ttagggaata
     5341 gtatctttat atttgttcct tttggcaata ttatactttc ttcacctatt aattttctaa
     5401 gtcctttagt atttcttaag aatatttctt ctgtatctcc tacctctaat acttctccat
     5461 tttccattat tgctactctt ccacaaatct gctttataac ttccatttga tgagttacaa
     5521 ctattattgt tattcccaat tttttattta tatcttcaag caatgaaaga attgactttg
     5581 tagtatttgg gtctaatgct gaagtcgctt catcacaaag aagtacttgt ggatttagtg
     5641 ctaaagctct agcaattgca accctttgtt tttgtccgcc acttagattt ctgggtttat
     5701 catttttctt ttcagatatt ccaactactt ctaataactc taaaactctt tttttaattt
     5761 ctgcctttga gtatccaaaa cattctaatg gcaatgctac attatcaaat acagtttttc
     5821 tttcaagcaa tgaaaagtgt tgaaaaatca ttcctaactc ttttcttaaa tctctcattt
     5881 gcttttcatt caatgacttt acttctttat cagaaacaag tacactgcct tcttggtatt
     5941 cttctaatcc gtttatacat ctcagtagtg ttgatttccc tgcaccacta tgacctatta
     6001 ttccaaaaat ttctccagat tctatttcaa tacttacatc tttaagcact tgaatctttc
     6061 cataatactt attaacattt tttatgctaa tcataaaatt tcctcccata tattctgctc
     6121 agtttttatg tttatcctct cttatgtatt acaacaaaat aaaagcccga gtataaactc
     6181 aggcttagaa agctatttat actcatctat caactatatt agttgcagga tttagcacca
     6241 atgtatataa aatatactgg ttgccgggtt tcaaagggcc agtccctcca ccactcttga
     6301 taagacgtta tttaatttaa ttctattatc atttgttgct atgttatata tgatatattc
     6361 attaatagta tttgtcaata aatttattta aattttttat tttttattat cgttaattaa
     6421 ctatttttaa ttagttttct agctcctaac actttatatt ttaatgtcta tataactata
     6481 gaacatcatt ataagaaata aattaaataa catcaatcta tctaaatcat ctgtaaagta
     6541 aattaagaaa attaacattt tatccaaact ctatcctact attaaataaa ataatttaaa
     6601 tacaatgatg attatatagc tctagtactt tagactttat caaatgttta agttctatag
     6661 gttctattat tgtagcattt ttcccaaaag ttaaaaaata ttcagccacc cagttaagtg
     6721 tagattttgg tatatacata tctatgtatc cacctttaga caagattttc aaccctcttg
     6781 ccaacaaaaa ttctgtttca caacgtttta ctccctcatc tgtcaaatta attttaatat
     6841 gataatctgt acctacttcc atattcttaa gataatcatg tatagaagat aaaaccttat
     6901 atttcttact tgataagttt tctatactca aaatcttcac aattctgtct acacgaaatt
     6961 ccttaataga ttctgatttt atacagtaag ctggacaata ccaaagtcca ttcatggcat
     7021 acaaaccaat aggaacaatg gtacgtacac tatttttttg ctttgatgaa tattcaattt
     7081 taattgtaaa ttcattcatt actatattaa acaattcttt caataatggt gttgaacagt
     7141 gcctatctgg tgtccaaaaa acaaatttac gccttatatt ctcaatatta tattgaatat
     7201 catttggtat acaatttaaa aattttttta aaacagaaat agtttcttgc tcaaatggca
     7261 aatcattata atattctagt gactgatatg caaagaatat agattttacc tcactttctg
     7321 aaaatgcaat cggtggaagt atacgctctt taagtacatg atatccacca gccgctccaa
     7381 cttttgaata taatggaaat ccaatactct ccagcacctg taaatctctt agtatagtac
     7441 gttttgatac agaaaattct tctgctaatt ctcctgctgt aaaacttctt tttgcattta
     7501 ttgttatcat cagttcaatt aaacgctcag ctcttgacat atttatactc ctaatttaaa
     7561 tatagtgaca ataattgtca ctattatatg ataagatatt cttgcatcta aagcaataat
     7621 aatagtaaag aaagaaggga ttgatacttt atggaaaatg atactattaa ggctgatgat
     7681 attctcaatt attgtctatc aaacttagat gatgttgtac taatggatag ttggggggaa
     7741 cgagcaattt actacaatcc taatggtgtt ttaaagcgag gggtatatgt tcttaccatt
     7801 aaggaaaaag acagtaataa tgataaaggt tcgttagtta gtcgtccaaa tgtataccgt
     7861 gtgaatatag gattaaaaaa agaaactttt attgaaatgt ttggatatat tccaaagcgt
     7921 ccaggtgtag gtcaaatagt tgatatggat tttgatttta caaaattgga cacaatcatg
     7981 ccacatccta tctactcatg gatgggatgg atatgtgtcc taagccctac tgaaaagaca
     8041 tttgagaact ttaaaacttt aataggagaa tcttacaatt ttgcaaaaca aaaatttaaa
     8101 aagagaaaaa acaagtaaaa acgtgtgaaa ataacctcta aataaattat aataaaaata
     8161 ttaaggttac aagataaaaa ttaatatctt aataacatgc aaaaaagcga agccaaaaaa
     8221 tgtattattt ataaggcttc gctatatctc aaataattta tttcaaattt caaaactatt
     8281 tattctatta tttcttatta tttcctgtta ttcctaatct atcattcata tcttttatca
     8341 tttttctaaa accactacct atatcatttc caacatcttc agttccatga actaatttta
     8401 taaagtcatt ttgacctttt ttcatatctt catcagatac cttatctaat ttttctattc
     8461 tcttatctat atcttttact aatctttccc cttcagcgat taggtcatca ttcattttct
     8521 ttaactctgt atcttctgtt ttaccatctt ttttaagaga tgttacaaat gattgtattt
     8581 tatctctact atctttatat gcagccttta catcacttag atatttttca tttcctggat
     8641 attctttatc tttaaactga ttatttatac tttctggagt atcataaata gaatatctat
     8701 ttagacctgc tatataatca ttatagcttg ttgaataata attcatatat ccttcattat
     8761 tatttacaga ttcattagga ttttttcctg tattttcact tggtttttca ttatttttag
     8821 tacatgcagt tatacctata gctaatacta gtgctaatcc tacaaataaa gttcttttta
     8881 atttcatttt tattaccctc ctagatgttt tatactataa aaaattttaa taattttctt
     8941 tcttaattta gtatttctat atttttatat tttatttact aaaattaaac aagttataat
     9001 ttattttttt ggaaatcata atgaataaat aattaaataa aaaaattata attttatcat
     9061 tgatattgat tatcattcat agtataatct aattacttga tattaatttt catttgaagg
     9121 aggttatctt ttatgtcttg ttgtaattca caattttgtt gtaaaaataa aattgaatca
     9181 tcatatataa aatggctttt agaagtttta ggtccagtct ttttaggctc taaacctgct
     9241 gaaataataa atatttcgtt tactgatgta aatagagagg aaaaaattaa tgatatttat
     9301 aaatatctat ataagtgtaa gaaaatagat tttatagtta ttgataaaga aaaaaaggga
     9361 ttaaaaatct tatttgtaaa taaaaaagca ttatcagaaa aattaaaatg taaaaaaaca
     9421 gtaaactttt taaagttttt aggatacaaa caaaatacga atgttaatgc ttatttagag
     9481 catttagtag ataaacttaa gagtgatgtt tttcctgatg aaataggtat ttttttagga
     9541 tatcctctta aagatgtgat tggatttatg ggatatagta attatgaagt ttctatgatt
     9601 aaatattgga aagtttatgg tgacacaaaa caatccgagg acacatattc gaagttttta
     9661 cttcaccgta agaaaatgag aaaattacta gactacataa gtgtagataa aatagtaagc
     9721 tgtttttaat tgtgtttttg tataataata aaaatacacc ctcttttacc atacagtttc
     9781 cataataaat aagggtgtat tttccacttt aatattttaa agtattctaa ttttgattgt
     9841 acaacatatc tttaatattt attttctttt gttgcattcc attagacatc aaaaattcct
     9901 gtgtatcttc taattctttt atatccttgt ccgttatttt agaactaaag tcataccaag
     9961 aatacatttc ttttgtttca tctaaagata aaccaacttc tttagataca acatccatca
    10021 cttcatcctt gttttcattc atatatttta aagtttcttc atgaaccttc ataaatcttt
    10081 ttactaattc aggatatttt tcagcaaagt catcacttac agcagtaact attattccac
    10141 ttactaatcc ttctccattt acaatcattt tggcacctga atttatcgtt ttcaatgcca
    10201 ctggacctgc aagcattgct acatcagcac ttttactttc taatgcagaa gcagcactag
    10261 gaatatccat atttacatat tcaacatcat ctatagtata gccttcttta gcaagtgcag
    10321 caatcaacag ttgatgaagt atagtccctt ttggacctac aattttttta ccttttaagt
    10381 cttttataga tttaatatta tcattattag ttataagcat atatcctttt ggtgaacggc
    10441 tataaatatt cgtaatttta agaccaacac catttgatgc agctattatt gcagacgttc
    10501 ctcctaatgc atgtaaaaag tctagctcac ctgctgctaa tgcttgagtt tgctctggtc
    10561 ctgttgtcaa ttcatggaaa ttgactttta tattgtcctt tttaaattcc tttccaaata
    10621 aatcatcctt tttttctaat atactaggaa cattaagtgg tgattttaca taagttaaat
    10681 ttatttcctt aggtaaacta gcctcttttt ttgcaggttc atctttctta tcatttgttg
    10741 ttgaacatcc tacagtccca accaacacca ttattaaaga tgtcattact gctaaaatct
    10801 ttttgtttct tttaatcaat taaaactccc ccaattccct taatatttct tttttcaagt
    10861 ttatataata gtttttagtt aaatctctat tatattcatc ttcgacacta aattgtttaa
    10921 tccttttatt tttactaaaa acaattattt tctttgctat ttccatagct tcttcaatat
    10981 tgtgagttac aaatactaca ccttttttag tatttttatg gatatttaca acttcttttt
    11041 gcatttttct tcttgtgaag taatctagtg ctgcaaatgg ttcgtccatc agcaaaatat
    11101 cgggattgaa ggaaagtgct cttgctatac ttactctgtg agccattcct cctgataatt
    11161 catttggata tgagttttta aacttttcaa gcttcatcat ttttaaatac ttgtctaaat
    11221 caactttatt tctattatct ttttcagtat gtaaaagtat attttcactt acattcagcc
    11281 atggcataag tctactttct tgaaatacca ttcctacttt tggagtacac tttttatcat
    11341 tattaaaaaa atatacgttt ccactagtag cattttcaag ccctgctaaa attctaagta
    11401 gcgttgtttt cccacatcca ctttcaccta atataacagt tatctcttca ctagatatat
    11461 ttaaagatat attatcaaga actaaatgct ctttattatc aactaaatat ttcttatata
    11521 tattctctag caaaaatcca cttctaacca tttaaatcaa cctgcttccc tttgctaaat
    11581 tttgttatta tctttgaaaa tagataatct gaaagtatac ctaaaaatcc aataacaaaa
    11641 attcctacca atacaacatc agttctagac atatcttttc catcagaaat caaatatcct
    11701 agtcctgaag aagctgcaat aagctctgct ccaattatag ccctaaaact atacccaagc
    11761 cctaaactaa gtccaacaac aatatccagc atagaattag ggattattat tttataaaaa
    11821 attctctttt tagataaatt aaaagattta cctacttcta ttaatttatt gtcacaattt
    11881 tttatacctt tcaatgtatt taaaaatatt ggaaaaaagg atgccaaaat aataattata
    11941 attttagatt cttcacctat accaaaccat aaaataagca ttggcaccaa agctagtgga
    12001 ggtgtacttc gcataaattc aaatagtggt ttgaaatatt catacatttt tttatttgct
    12061 ccaaataaaa ctccaagtgg tatagccatc aatgaactta ttaaaaaacc tattaatatc
    12121 cttcttacac tggcataaac atttaagaaa atagaaccgt catatatcat ttttataaaa
    12181 gtatctaaga ctatttcagg aggaggcaga atatagctac tccatatacc caaaacacat
    12241 actatcttcc ataaaattaa aattattaaa aaaatataat atcctttaag ccttttatga
    12301 attttctcaa acatataaaa ctccttttaa catatctatc cctgctaccc tttttatttt
    12361 ttaatatctt aaataataat taagctatat cataagattt aatctatgcc aagtcttaat
    12421 ccacctgaac tcattctatc agaaggtgta gaatctgttt ttaagccaga caatctttca
    12481 gatggttgta taatgactat ttgtccaggt tcaacaaatt ccatatgata agattctcct
    12541 gaagtctgac caataaaagt cttccatgat agttttgata ctttaggata tggttctctt
    12601 ccagtccaag ctactaatgc atctgggtca acacaacaag gtccttcaag aattagagga
    12661 ttaccatctg ttattatagc aacatcttga ttttgacctg ttttatttac taaatgtgta
    12721 gtaaaaagac ctttttgaga tattacccct gaaccaatca attttacact gtaatttaat
    12781 tgttcagaaa atgccaataa gttttcactc tcaacataaa tggaatcacc tgggtcaatt
    12841 gatattacat ctacatggta agcattttga gctaggtaaa tttcaccttc accttcaacc
    12901 accattattg gcatttgttc tcctgtcaat gacctctgaa catgacctaa taatgctctt
    12961 cccaatccac cgccattact tggacctaac agcaattttt caaaattaaa atttccttta
    13021 aaggctacca tagcaccttt ttttgcaaaa aatttaccac ttccccttgc tatacacata
    13081 agctcgtttg taacatcaaa tttaaacata atattcactt atattgctta atttagcaat
    13141 aatccctcct aatcatatat ttatatataa ttatacagta aaattacagc atagtctaac
    13201 ctacttcaaa tatgaaattt gttatttata caaaaaataa gaacctgata ataaagcaat
    13261 ttaacttttt atcaggctct tatcctattt atctcttagc gctatgacaa ctcttactac
    13321 ttgagcatcc agaacaacca catccacaac tgctaccatt cttttttgaa tttttaacca
    13381 tatacattac agctagtgcc attaatacta ctacaatagc tgctataata aatgtagcca
    13441 tattcatcac tccttattta tatgaattaa tttttcatat aacttaattt acccattttt
    13501 tcttttttca tatttttatt tgaataagtt aaaactacaa agactactgc tactgctata
    13561 aatatagata ttattgcccc agcaatactt cctccagtac ctaaaactaa acttccaact
    13621 tgatttataa gaagagcaat tatatacgct gttaaagttt ggaatcctaa tgtaatccat
    13681 gtccatttcc atgaacccat ttctcttttt atggctccaa tagctgcaaa acaaggtgca
    13741 caaagcatat taaatgccat aaatgcaaac gcactagcta ctgtaaacat acttgcaact
    13801 gaagcaagaa gtgttgggtc ttgctctgtt gcatctgata taccaaataa aactccaaat
    13861 gttccaacta cattttcctt agctactaga cctgtaactg ttgctactga tgattgccaa
    13921 tttccaaatc caagaggagc aaatattggt gcaactatat ttcctaagct agctaatata
    13981 ctatctccag catctaccat ttgtaatgac caattaaatg attgtaagaa ccagattact
    14041 ccacaagcaa caaatataat tgttcctgct ttgattataa atgcttttcc tctatcccac
    14101 atatgtatta aaacaccttt tgcacttgga atatgatatt gaggtaattc cataacaaat
    14161 ggagatggtt ctcctgcaaa taaactagtt ttctttaata taattccaca aatgattatc
    14221 attgcaatac ctaagaaata cattgatgga gctacccatg aagcacctcc aaataatgcc
    14281 cctgcaaata atgctattat tggaatctta gctccacatg gaatgaatgt agttaacata
    14341 atagtcattt ttctatctct atcattctct atagtacgag ttgacataac tcctggaact
    14401 ccacaacctg aacttataag cattggtata aatgattttc ctgaaagacc aaacttacgg
    14461 aaaattctat ccattataaa agctacacgt gacatgtacc cacaatcttc taatattgat
    14521 aataataaga ataaaagcat gatttgtggt acaaatccta gtactgctcc aactccacca
    14581 attagaccat caactacaag tccttgtaac cactctgcta catttaaaga agctagaaaa
    14641 ttacttacat tgccttgaat aatttctcca aacagtacat catttgtcca atcagttgct
    14701 atagtaccca atgaacttac tgctatatag taaactcccc acattataag tgcaaaaata
    14761 ggtaatgcta gtatacgatt tgttacaatc ttatctatct tatctgatac agtttcctta
    14821 ccttttcgat tcttctttat tatacttgaa attattgaac taataaattc ataacgattg
    14881 gctgttatta tactttcact atcatcatcc aattcatctt cacaatttct agttatttct
    14941 tcaatactat tcaatattgt tttagaaata tcaagttttt gtataacatt ttcatctctc
    15001 tcaaatagct ttatagctaa ccagcgtgtt tctacatcta tatatggagt cttttcttct
    15061 attatttttt caattttttc tattgcatcc ttactttcat caacaaaggg taatttgaaa
    15121 tttaatttat tgttactact tgcaagttca actgcctttt cagcagcttc cataattcct
    15181 tgacctttaa ctgctgttac ttctaccact gggcaaccta taacttcaga tagttcttta
    15241 atatttatct tatcaccatt cttatttaca atatccatca tattaagagc tataacggtc
    15301 ggtataccta gctctaaaac ttgagttgta agatataaat ttctctctat atttgatgca
    15361 tctacaatat ttattactgc atccggctta tcatctagca taaaattacg tgtaactact
    15421 tcttctaatg tatatggaga taaagaataa atacctggta aatctactat ttccacatct
    15481 ttatttcctt ttaattttcc accttttttt tcaactgtta ctcctggcca gttaccaaca
    15541 tattgtgatg agcctgtaag cccattgaac attgttgtct taccacagtt aggatttcct
    15601 atgagaccta tttttattga catttccatt ccctccatta ttatttaaat acatcactgt
    15661 ttgtgataca gaaaatcatt ctcattatat gattacatta accctactct aattaatctt
    15721 tattattcca caataatttt ttctgcatct gacttacgaa ttgatagttc ataatctctg
    15781 actgttacct caattgggtc accaagagga gctacttttc ttataaatat attaacacct
    15841 cttgtaatcc ccatatccat tatacgtctt cgtgtagctc cttctccttc taactttttg
    15901 acttttacag tctctccaca cttaatatcc ttcagtctgt tcatgtcatt acctccctat
    15961 attattattc tacttgccat ttttttatct aatgcaactc tagtatcttt tacgtctatt
    16021 attaagttac catctatctt agaaactatt ttaacatttg tacccactac aaaacctata
    16081 ctatttaaaa aagtcctcgt cttatcactt cccgaaattt tctttataac tacttcttca
    16141 cctgtatttg ataatatcaa tggcatcata taatatccct cctcttaaag taatttaaat
    16201 ttacaagcaa tcgttatctt atatataaat tacaatacct taaatcttaa tctattaaaa
    16261 taatatccta ttttttcctt ctttttacaa ttcctaattt tgatttaagt ctatcacaat
    16321 agtatatttt ttgcaagtga ttattgtatt cttttgattt tttttctcat ttgatttatt
    16381 ttattaataa aatcgtaaaa attaatacaa aaataaagat gctctaaaat agtatactca
    16441 actaatatta aagcaccttt attatatatg tcattattta cacattgctt tattcttttt
    16501 taaaagcgat tttttcaaac tcttttacag gcattggctt agaaaacaaa tatccttgta
    16561 ccatatcaca cccaatctgc tttagaaatt ctacttgcga aatagtctct acaccttctg
    16621 atattacttt cattttcagt tcctttgcca ttttaatgac attagatata acaatttttt
    16681 cattattact ctcatctttt tctgtaaaaa atgctctatc tagttttaat atatcaacag
    16741 gcaattcttt taaaagatta agagaggaat atcctgtacc aaaatcgtcc atagagatta
    16801 caaacccctt ttctttcaaa ttgttcattg tatcaagtaa tccttctata ttatctaata
    16861 ctgctgtttc tgttagctct aattctataa gtcttgtcgg aattttgtat ttatctacaa
    16921 ttaatttaaa cttctctata aaattactat ttacaaaatg cattcgagac aagtttacag
    16981 atataggtac tactttttgt ccttcatcca tccattctct aatcttttta cacacttcct
    17041 caaatacata gaaatcaata tttacaataa aaccattcct ctcaaataca ggaataaatt
    17101 ctattggtgg tattagacct ttttgtggat tatcccaacg aacaagagcc tctgccccta
    17161 taatttgata atctgaaaga ctatatttag gttgaaaata aacaataaat tctccattat
    17221 taagagcatc taccattgaa ttttcaattt ctttctcttt taatattttt ttgtgcattt
    17281 ccttatcgta aaaagcaaat gaatttttat gaccaccttt tatagttttt cttgcagtat
    17341 tagctctatc cataatagta tttatgtcat tatcactttt acttatttta tatataccac
    17401 aatctaaaat caatttataa tagtttattt taggattact aagtatttga accttattgt
    17461 aaatattgtt taaccttgtt ttaatatcat catctgtctt atacttaata agaagaacaa
    17521 aatggtcagc agaaactctt gcaaaaattt catcttcctt taattcactt gcaatagtat
    17581 ttgatatatg tatcaaagta tcatttccca tatcatatcc aaacatatca tttatatatt
    17641 taaatttgtc aacatctata taaaacaacg catattcttc tggattattt ttagcaaaca
    17701 gtttattaga atttatttta aatttatcta tactatctgc tcctgtcaat gggtctgtat
    17761 atgctatctt ccttagataa tcctctttct tctttttatt ataaattata tatttaatag
    17821 caattaagaa taaaatacat acaaaagaaa taatttgtat tgaatattct ttaaaaatca
    17881 caccaaatgg tactttataa ggtttgtcaa tagtattaga atatatacaa tcacttaatt
    17941 cttcttcagt aattttgtct attgatttat ttaaaataga cactaatata gggtcagttt
    18001 tatttgatac ccctatacgc atcccatagc ttgtatctaa aacagatata acagataaat
    18061 tctcactctt tccttctctt aaaagttcat caaactgata agagctaagt atcattaagt
    18121 ttgctttacc ttcatttata gcgtttagac aactttctac actcttataa gatgttgtct
    18181 tagcattttc aaaactcttt tctatataag aatcaataga cttataacta ctagttaaag
    18241 caatatttaa atcattattg agattggtat ccaattttcc taccataatc attggaatat
    18301 ttatgtatgg atttgtcaat ttcatattaa atcgttttgc cttatcattt tcattgattg
    18361 agccacatag catagttgcc ttaccacttt ttatgtaatc aagactctca ttataatttt
    18421 ttgttttaat atattcaaat tttataccac atttttttga tataattgac attaaatcgg
    18481 aacttatacc tttaaatgaa ttgctttctt tatcataata ctctacagga gaccactctg
    18541 aatcatatac catagtaagt tttgggtttg ctttaataaa attcatttct tgccgagtaa
    18601 atgctatgga attattgacc tctcttttga aatatttttt atataattcc aactcataat
    18661 acatatcatt tatttttaat tcttttaaag cataatctaa ttcttttact aaattaggtt
    18721 tatcttttgc agtagcaaaa taaaaaggtt ctactgaaaa tttgctaact attttcattc
    18781 ctttagcatt tataatactt ccacaaacaa atgcatctgc ctttttttca ttgacggact
    18841 caattaattc atcaatagaa ttacagtata tatattcaac tgaaaaatta ttttcttctc
    18901 tatacttctc aaatgcagta ttatgaaagt ttcctctaag tacagctact ctcattccct
    18961 taaaggaact tatatcttca taaaacatat tattgttact atcctttgta tatagtgcac
    19021 tatattcata tcctgaactt agtttaggaa aattaaatag tttatttctt tcatcattct
    19081 tctgtaaagg tccgagtaag tctatttcac ccttttctag tctgtctaaa cattcttccc
    19141 aagacccttt tacaaattca tattcccatc ttgtatattt tgagatttct cttagataat
    19201 cataaccata tcctgtataa gaatgctctt ttgatgagga tattattccg tagtcatcac
    19261 aatatccaac tcttattttt tttgaatttt cagactcatc agcaaagcat agtatattgt
    19321 taaaacatga tataataagt aaaaatatca aagaaagtat caagaagtgc tttttaaatg
    19381 ctttcatatt taaaaacctc caatttgcca acttacttta acttatttgg ttttctagtt
    19441 gtaactactt ttattgtaat
//
LOCUS       DAELNY010000062        19418 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.062, whole genome shotgun sequence.
ACCESSION   DAELNY010000062 DAELNY010000000
VERSION     DAELNY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.062"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(196..918)
                     /locus_tag="KPE55_001863"
     CDS             complement(196..918)
                     /locus_tag="KPE55_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0492948.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(977..1654)
                     /locus_tag="KPE55_001864"
     CDS             complement(977..1654)
                     /locus_tag="KPE55_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0492949.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1682..2497)
                     /locus_tag="KPE55_001865"
     CDS             complement(1682..2497)
                     /locus_tag="KPE55_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0492950.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2723..2933)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3610..5676)
                     /locus_tag="KPE55_001866"
     CDS             complement(3610..5676)
                     /locus_tag="KPE55_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0492951.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5753..8782)
                     /locus_tag="KPE55_001867"
     CDS             complement(5753..8782)
                     /locus_tag="KPE55_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0492952.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(8835..10295)
                     /locus_tag="KPE55_001868"
     CDS             complement(8835..10295)
                     /locus_tag="KPE55_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG0492953.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10387..10761)
                     /locus_tag="KPE55_001869"
     CDS             complement(10387..10761)
                     /locus_tag="KPE55_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492954.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(10937..11677)
                     /locus_tag="KPE55_001870"
     CDS             complement(10937..11677)
                     /locus_tag="KPE55_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0492955.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11698..12486)
                     /locus_tag="KPE55_001871"
     CDS             complement(11698..12486)
                     /locus_tag="KPE55_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG0492956.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12574..12664)
                     /locus_tag="KPE55_001872"
     tRNA            complement(12574..12664)
                     /locus_tag="KPE55_001872"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12628..12630),aa:Ser,seq:gct)
     gene            12923..13687
                     /locus_tag="KPE55_001873"
     CDS             12923..13687
                     /locus_tag="KPE55_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0492957.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(13968..15083)
                     /locus_tag="KPE55_001874"
     CDS             complement(13968..15083)
                     /locus_tag="KPE55_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0492958.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15124..16650)
                     /gene="glpK"
                     /locus_tag="KPE55_001875"
     CDS             complement(15124..16650)
                     /gene="glpK"
                     /locus_tag="KPE55_001875"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0492959.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGIPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            16943..18040
                     /locus_tag="KPE55_001876"
     CDS             16943..18040
                     /locus_tag="KPE55_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0492960.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPDDCYKFLDILKSKLEIKKVI"
     gene            complement(18531..19043)
                     /locus_tag="KPE55_001877"
     CDS             complement(18531..19043)
                     /locus_tag="KPE55_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492961.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT         6238 a         3462 c         2019 g         7699 t
ORIGIN      
        1 atttattaaa aatcggatta gtaacggaaa ttaattcggt ggaaaaaaag agcttacccc
       61 gagctctttt ttttctgcaa aaaatcgtaa aaataaagtt aatattaata taactttaat
      121 tttaaaatta atattaacca tcactaaaaa aggatgtatt gaatttctaa tatcaataac
      181 atccttttta tataattata gcacttttgc taaaaatgct tttgttcttt catgttttgg
      241 atttccgaaa acttcttctg gagtattatc ttcaagtata cttcctccat ctataaaaat
      301 aactctatct gcaacttcct tagcaaatcc catctcatga gttacaattg ccattgtcat
      361 accttcttta gcaagttctt ttataacatc caaaacttcg tttaccattt ctgggtcaag
      421 agcagatgtt ggctcatcaa acaacatcat atctggttcc atagcaagtg ctctagctat
      481 tgctattctt tgcttttgtc ctccagataa ttgtgatgga tatgaatctt ttttatctaa
      541 aagaccaact ctactcaaca aaatttctgc ttttttttct gcttcttcct tagtcatctt
      601 ttttaattta attggtgcta atgttatatt atctaaaata gtcttatgtg ggaataaatt
      661 aaagttctga aacaccatac ctatattttg tctaacctca tcaatattag tttttttatc
      721 gaccaaattt tttccttcaa atattatttc tccacctgta ggtatttcta gtaaattcat
      781 acatctaaga agtgtacttt taccagaacc acttggacct actataacca ttatttcgcc
      841 tttagcgatt tctaagtcta tattttttaa tacatttaaa tctccaaaag atttacttaa
      901 attttttatt gtaatcatat tatcactttc ctttttacta ttctatgttt tccctaaatg
      961 ctaaacatgc tctcatctag ttagacacag atagtttctt ttctaataat ccaactaatc
     1021 ttgatagagt aaatgttata acaaagtaaa ttgctgctgc tactattaat ggctctaatg
     1081 ctctaaatgc tgcatttttt actatatcag cagtacgcat tatctcaaca actcctatta
     1141 cagatattat tgaagattct tttacaagtg taataaactc atttgctaat gaaggtaata
     1201 tattttttat agcttgaggt attattatat atctcatagt tgaccaataa tttaatccta
     1261 atgatctact tgcttccatt tgtccattat caactgattg tatacctgat ctaagtatct
     1321 ctgccacgta tgcaccagag tttactgata atgcaaatgc actagctata aattcacttg
     1381 gtataccaaa caacataggg aatcttattc ctaattgagg taaaccaaac catactatat
     1441 atatctgtac taataatggt gtccctctta aaacctcaat atagattgat gatattgctc
     1501 ttaatacttt gctctttgat atttttgcca tacagataat tatacctatt ataaatccaa
     1561 agcataatgc cacaagagaa attgatactg tcacacctgt tccttccaaa aaagaagtcc
     1621 caaatctact taaaaaacta aaatctaaac tcataatttc atctcccaca taactttctt
     1681 actaatttga agcagcttca gtagatgctt catttaaaaa ttcttctatc ttcttgccat
     1741 cttgtaattc ttttatcttt ttgtttaata attctataaa atctttgtta ttgtcagatt
     1801 ttttgattgc tgctgccata ccttctgtta catctttccc aacttcagta tttgccattt
     1861 ttattccatc atatttttta acatttatta aagaaactgc ttcattagta acaactgcat
     1921 ctatgtttcc attttttaat tctgtaatta aatcaggtat tgctttcaaa gattttgtag
     1981 ttgtcatttt taaagtgtta actacatatt cttcttggat acttcctgct tgaactccaa
     2041 ctctaagttt ctttaaatca tcttcagttt ttactttgtc tatatctgca tctttaacta
     2101 taactgcatt tctacttaaa taatataatt ctgaaaaatc aacactcttt tctctctctg
     2161 gtgttggaga catacctgca agaacaatat caacttggtc tgcatttaaa gcacctatta
     2221 atccatcaaa tgacatatcc ttaatttcaa cctttacacc aagctctttt gcaaattctt
     2281 cagccaacat tatatcgaat ccttttattg aatcttttcc atctactacc ttatgaaatt
     2341 caaatggagg aaattctgca ctagtaccaa caactaattt tccattgtct tttatttgtt
     2401 ctaactttgt tttttctcct ccaccactac atcctactag cgataatgtc aatcctaata
     2461 ctaatcctaa acttaataat tttttaaata ctttcatttt aactacccct ctcttttaat
     2521 tataatttcc ctcagatttt aatacttaaa tgtttcgagt tacaaaaaaa gtctctcagt
     2581 ctttaatgac taagagacga ctttaccgcg ttaccactct tattgataaa tacttatatt
     2641 actttcaaaa tttgtgactg ttagcattta agctgtatgg atatttttat ttttaaatca
     2701 aaaaagtccc ttagctttac agctaagaga cgaattatat ccgcgttacc actctttttg
     2761 ataagtaagt acttatcctc tcaatccctt aaggcgggta aaccaattat catactaaag
     2821 tttctgataa tctcctccag agctctcttc acatagactt cattgctagg cttacaccaa
     2881 cccctaactc gctgtaaatc catatatgct actttctcta tcattggatt tgtttcctta
     2941 aattgttaat acaattgtac ttccttataa tatttttgtc aataagtttt ttattttttt
     3001 aataattttt acttgtaaat tatctaaata ctactgttta tatttattga tattagtttt
     3061 gtaattataa atagtatatt tcatattttt agctatttct taaataatac taaataatat
     3121 ctttcacatt gttgcttttt cttaaattac actaaataat atctttcaca ttgttgcttt
     3181 ttcttaaaca atactaaata atatctttca cattgttgct tttcttaaat tacactaaat
     3241 aatatctttc acactgttgc tttttcttta ataatactaa atagtatctt tcacactgtt
     3301 gccctttctt taataatact aaataatatc tttcacattg ttgctttttc ttaaattaca
     3361 ctaaataata tctttcacac tgttgctttt tctttaataa tactaaatag tatctttcac
     3421 actgtttctc tttctttaat aatactaaat aatattttcc acattgttgc tttttctaaa
     3481 caactataaa taatatatta aacccttatt tctttttcta ataagttaaa taatacattt
     3541 tacacatatc tatttgtctt gcacataata aataatacat tatatttcta tctaaattta
     3601 gattctaaat taaatagtat aaattatatt cttcaataaa caagttttca aatccaagat
     3661 tcgctctcta tctttaaact ttattgtaaa cataattcca tcttttttaa ttatttctcc
     3721 ttcatgaaat tttttatggt ataccttatc ccctacacca atactgtcaa tttctttttt
     3781 cgttggagat tttatatcat taataaataa tgatttatca actttcttac catacttatt
     3841 taaagtagag cttatacata acttttcttc agctcttgta atagcaacat acataagtct
     3901 tctttcttct tctaattgtt cttctttttt ctcttcctta caaatatcat atgatttttc
     3961 atgaggtatt gttccttcat ttacacctat aatataaaca tttctaaatt ctaatccttt
     4021 tgcactatgc atagttgtaa atatgacacc ctcagtctgc ttattcttgt tattttccgt
     4081 taactcagtc tttactctat caatatgctc taaaaactct gtaacagtat taaaattagt
     4141 tgctgaactc tcaagttcat ttaatatctc tacaagtcca ttagtcttta tctttctgtt
     4201 gctacaataa tctaaaacat atctatcata atcaagacta gttctaatat aagatattgc
     4261 atttttagga tttaaagtat ttaaataact taaatctatt tccaaatcat ttattgtttt
     4321 aacttgttta ggatgaagat tacacttatt tattaatgct gttataaagt ctttttcatc
     4381 cttaaccata tttatactgt ctttggatat atatctaaaa ggcttgttta taattctcaa
     4441 ccaatcttta tttgattttg gattaatacc tattcttaaa taggctagta tgtcttgact
     4501 tgcccaatgg tcatatattg taattactga gtcttttact acaaatggaa tcctcatatc
     4561 cataaataca tctactaatg ctcttgactg tatattagtt ctataaatta ctgcaaaatc
     4621 tgaatactct acataatctt tttgtatttc gtcaattatt tccctggcaa tatataaggc
     4681 ttcttcctct gaatcatgcg gagaaatata ggtaacactt cctccatccc cctgactaca
     4741 cttaatgatt ttttcatatc tatttttatt tttgtcgata agtctatttg cagtatgcac
     4801 tatctcactt ttagacctgt aatttatatc taatattatt tttttagtat tattaaagta
     4861 ttgttcaaat tcaagcaaaa aatctggtct agcacctcta aatccatata tactttggtc
     4921 ttcatctcca actgcaaata tattgtttaa tgggctacaa attaacttta aaacttcaaa
     4981 ctgtacttta tttatatctt gaaattcatc tatcaatata tatttataca catttctaac
     5041 catttctagt gcagacttat tgttcaaaag taaataataa gttcttataa gcatatcatc
     5101 aaaatctatt ttattaactt tagatttttg ttcttcgtat aagttgtata cttttaaaaa
     5161 ctcatctttt gttaatactt ctgaattaaa ttcattttta tccataagtt cattttttac
     5221 ataggaaatt tcatttataa cttgtcctat attctcatca tcatcagcat tctctatatt
     5281 taaacttttt aggattgcct tgattgtcat tcgctttgac ttttcatcaa atatactgtc
     5341 taagttgtat ctttcaaagt atcttagaat tttaaaaaat acagagtgaa aagtaccata
     5401 ggttacctta ttaagtctta tgtcatcact taaattcaaa gctctatttt tcatttctat
     5461 tgaagatgcc ttggtaaaac ttattgccaa gattcttgtt ggtgctatgt ttttgtttac
     5521 aaccatattg gctattctat atgtaattac tctggtctta cctgaaccag gccctgcaag
     5581 tatcatgcaa ggcccatcta tatgctcaac tgcttctaat tgattttcat ttaactttct
     5641 tatatctata tttgttgtga ttattagctc caccaccttg atttataatt atattatttt
     5701 ctttttaatt ataaactaaa agttcataaa aataaattta atttttcctc tattaaacca
     5761 catcattcct attttcatga catatcttga gttctttttt aattttgtcg cttatatctt
     5821 caggtataaa tgatggttta ctaatatagt agccttgcat ataatctact ccaagattaa
     5881 ttagtgtttc catttcacct tttgtttcta caccttctgc tatcacttta atatttcttt
     5941 cgttagaata tgagataaga ttttttaata ttttctgtct gttttcatct ttatcaatac
     6001 ctcttacaat agacatatca atctttacat agtctggcat tatatataat agtacagcat
     6061 ctccgttgta acctgtccca aaatcatcta aagcaatttc tatattccaa tggtctatat
     6121 atgctttctt tttctcaata aaatcttcat taatcttatc attctctgtt atttctagta
     6181 ctactctatt taaatattca ccatataaat cttcaatttc ctcaaaatct acttttgaca
     6241 aattattatt aggaatagaa tttaagaaca gtttgcaatc gccaaatttt tctttgtatt
     6301 ttttaaatga tttaagtgct ttaaaccatg taatacgctc aatttgatat aatttagact
     6361 gtgaacgagc taatctaatg acatctattg gcgaactaaa agtttttatt ttaggtctca
     6421 taagtgcttc atatgcaaat actttacctt tacaatctac aataggctga aatgcatact
     6481 caactaattg accatcaatt aacttgttta attcttctct attactcaat aagaatgaat
     6541 ccttattata tatattaata tcaaattcac taacttcacc cttgattgta tttttaattt
     6601 gatacatagc aaaatctgcg tatctcatta attcatcata gttggttgaa tcatcagggt
     6661 accaggaaat tcctgctgat gcccttattt taaattctgt tccatcatga agttttaata
     6721 aggtattatt catctgtttc ttaacagaat taataatatc tcttatacta tctttacttt
     6781 catatccata tataaataca taaaactcat cacctgaaag tctagatact ataccattcc
     6841 atgatataaa cttcttaagt atgtctgcag cacacctaat atattcatca ccagcatcat
     6901 gtccatatgt atcattaata tattttagat tatctaaatc ccacataata aatgctgcta
     6961 tcttaagttc atctttattc ttaaattttt cttgtatctg tgaatgaaaa gctctcctat
     7021 ttaaaagatt cgttaaaata tcaaaatctc tttcatattc tatcttcctt ttctcaataa
     7081 attcttttgt tacatcaaca acaacaccaa gaacctttag ctcatcttca aagatattca
     7141 ttcttatcca cttaagtttt ttatctttag attcaaactt atatatatat gtattttctg
     7201 tttcataact ttcttgcaaa tatttatcta gtccccttaa ttttttcagg aacttatttt
     7261 tttccatata tccatattct tcaaatttga tttcagtagt aaacacgcta aaaaattctt
     7321 ttgtacaaaa aacattctct gcgtttttgt ataactcaaa agctccaatt ggcatatcta
     7381 ccatttcaat aatctgtgat aattttgacg aagaatctgc aacatttgca ctgagcaact
     7441 ctatagctga agataattca tctatttcag atatatttat cttatcaaga tttactggtt
     7501 ttgatggatt actgtttcta actttgttgg ctaaaagaac aattggtttt gtaaataatc
     7561 ttgatgcaat gtaaatacct attagaccta ttaataaaga aattgctata gatattataa
     7621 ctaatttctc tacccttttt gaaaatgaca aaagactttt ttcttcaata atacccaaaa
     7681 gagcccaacg ttcattttca aatggagtat tggagttata taaatctaag tattgtacac
     7741 tagcataaat gttgccatta ccacattcat tatctttatt tttaatctta tatatatttt
     7801 tatacgattc cttctgctct aaattagaat gtaaaccatc ccaaaatttt tcttttaatg
     7861 agtatccatt tgaaattaca ttttcaaagg ccatatcatt atttctatct acacctaaaa
     7921 tataattacc ctttttattc ccatttatct ctgagtatgg aagtaattct cttaagtaat
     7981 ttacacttaa atctattcca agtactccat atacagttcc atcttcataa ataaggggaa
     8041 ctgtatatgt cataagccta tcacctgcat ttgtcaataa aaacttactt ccccagtatc
     8101 ctaaattaga acttgataca tttggattat caattgcagc tctaaatggt ttataaaaaa
     8161 aatcatctcc atcttgctca ttttcacttt taaatgaaaa cttaggttcc caataactgt
     8221 ccatagatat tccaaaactt cttgctatat ctgatgaacc tctctcaatt aacaaatcag
     8281 aattatcatt tggattaaac ttagggtcta aatcccttat atacaatcct gtcctactta
     8341 tttcaccttt actatctatt gacttatcgt taatattttt gtcacttagg attataaaag
     8401 ctcctgtaac agaatttttt ctcaaaagat atattaaatc atttgatatg ctttttataa
     8461 tatctttgtt aatttcttca ttgttgccta catcctcaat taaggcattt ttttcattta
     8521 atactttttt tacagaggaa ttaattgtag cttccgtttc acttgtattt gaccatcttt
     8581 gtaacatttc attttgtata taatttttac gattaattac tctttcattt aagatgtcaa
     8641 acgaattatc atttaatttc ttaatagttc ctccccataa aattgtggca caaaataaac
     8701 ttgtttgaat tagcataaca ataattaggg gaaatatcat ctttttcatt attgaattgt
     8761 tttttcttag attgttttcc atacccattc cacctatacc tttgtgctcg tcaacacact
     8821 aactgccaat tcatctattt ttttatagca ttttcaagct cagccttaaa actttcaaac
     8881 caagttttaa aatttttatc attattaaat tgtgctactg catccttctt agaagttcca
     8941 ctttttacta attcatttat tttatttgaa tcttctttag ctttatcaag cattgaatat
     9001 tcaagtattt gtctagcttt agttccacca tcaaatgctt tattagtata taaattacaa
     9061 ctgttaatct gttcaataga tgaggctatt gaaccttcaa ctttatcaga taaactcaaa
     9121 ttatctttct tcataacttc ttttataaca tccaaatcat tcgttttttt ctttactggt
     9181 aagtatccag aagcaataga gaattttata ttaggttctt tatctgtaaa ccattttaaa
     9241 aattctactg atgcatattc ttcttcttta gtagattttg ttacagccat tcctgcacct
     9301 tgttgtacag catattttcc ttctccttta aaacgtggtg cttgaaaaac agatgtttca
     9361 atattataac tctcattatc atttagcatt actctatctg gaaagtatgc tgctcctgaa
     9421 ctagaaccta caagagctaa tatatcacct gtttttgcat catcagaacg aaacttacca
     9481 taagaagcaa aatatccatt tatatatggt acatagaaat tatcccatag ttttttcata
     9541 actttttcat caatatttag tgtaacttta ccatctttaa cagaaaaaat ttctactcct
     9601 agttgtttac ttcctataat aatataattt gctaaagcat ctcttccaaa aaatgcttta
     9661 ccatcattag gctctttcgt taaactatct gtccattcat aatatttttt tgatgtctct
     9721 actaatcctt caattgtttt tatatcattt atatttgaat ttgtagcttt tgcaaattta
     9781 tcccaatcag ttttatttaa catcaaaatt tcagttgatt ttgcaattgg gaaaagttta
     9841 aactcatcct tagaatttat tctgccttct tctacatatt cgtctatgta ttcatttaat
     9901 tcttctttac ttatgtactt atccatatct actagtaacc ctaatttatc catttcatat
     9961 gcagtatctg agtatgctgc aaatatattt ggtacatctt cagaaccaac ttttttattt
    10021 gcagaatcta aaactttctc tgataaatca actacattac cttgactgaa tgcttcaaca
    10081 ataattcctt tttcagcacc tacagtgtca ttaaattcct taacaagctt atcaaaagcc
    10141 attttctgct gtccattata ataatgccaa atttgtattg atgtcggatt ttctgggtct
    10201 aacttataat tatcttcctt ctttaaacta catcctgtaa gtaataaaac aatcattgat
    10261 gtgataatta attttgcaaa cttacaccta ttcataaaat ctactccttc aaattataaa
    10321 atttataata gcattaaaac tgtaacttca ctatttatat taattataga acgtttattg
    10381 atatatttaa acaggattta caacatattt aaaagcaatt aagaaacata ctattacaaa
    10441 tatagaaaat aaagctttta cataattttt atttctaaat gcgccaaatg acaccaatcc
    10501 ttgttgaata gctactgtaa ttactaaaag tatcattatt atattaagtt gaaccagttt
    10561 aaataagtaa agcaatgaaa acactactag aagtacacat aatacaatac tagatttatt
    10621 aataatatca tcaatcttat ctaccttctt agattgctta ttaactttat ttaatttttc
    10681 tttatttaaa ttctcaactt ttttattata cctttctctt gttttatctt tatatttagt
    10741 atttttattt ttatttccca tttattttcc atcctctata aattttttaa ttgagcttat
    10801 acattattta aatattatat atttaagata ccatatattc ctacaaaaag gtaacttact
    10861 atgtatctat ttttgatttt cgcataaaaa agccatctcc tttagaatta attctgtatt
    10921 gagacagcta atttaactat ttatcctttg aaacattttc tacattatca tatttactat
    10981 caactaaaac tatatcaact cttctatttt ttgacctacc ttcttcagat gaattttctg
    11041 ctattggtct aaactcacca tatcctacag cagataattt atctggtgct atacctgcat
    11101 tgtcaataag aagttgaaca acattggttg ctctcataac tgataaatcc caatttgact
    11161 taaactctga attttttata gacatattgt ctgtatggcc ttctactctc atgtaactat
    11221 tcatttcatt taacatctta cctatttgaa taatcttatc tctagaatta tccttcacaa
    11281 tcgctttccc tgtatcaaat agtattgtat ctttaaggct tattactaat cctctatctt
    11341 gaactcttaa tgatactgaa ttagccatac cattttcatt taaatacttc tggatttctt
    11401 caccaacttt tttaaactta gcattttgta aagtatctaa atcattactt cctggttcaa
    11461 ctactggttc tatcttgcta ttaccacctt caataactcc agaagaacca ccaaaagcag
    11521 aatttagaga ttgagataat tgtttatatt tctccgcatc aacattactc atagaataca
    11581 ttattacaaa gaaaatcata agtaatgtta tgaggtctga ataagttaaa agccatcgct
    11641 cattgttttc ttctttttca tcttcatcat acaacataaa cattccatcc ttttatatta
    11701 ttcagctcta ccaccaattt cttcaagttc aattagttct tttgaattta aaaatatttt
    11761 taatttttct gtcattaaat tagaattatc accttcaagt atggataata ttgcttctat
    11821 caccaaaagt ctttcactta tttcctgttg atttatagcc ttaagttttg atgctattgg
    11881 taaccatatt aaattggcag aaccaacacc atacaaagta gccaagaatg caacagaaat
    11941 ttgaggtcct aatgcatctg gattacttga aagattacct aaaatatgta ctaaccccat
    12001 aactgtacct attatcccca gtgtaggtgc atatccccct gctgaatcaa aaattgctat
    12061 tccttcttta tgtcgttttg atgttatctg tatctgtgat tccaatatat ttcttatagt
    12121 aactgcatcc actccatcta ccataagttc aagccctttt ctaataaaag ggtctaattc
    12181 atcattctca cttatttctt tgtcaatact aagaagcccc tctttcctag cctttgttgc
    12241 aatttctttg aaatagatta tattttctat gttattaggc tttttatttt taaaagctaa
    12301 taacattatc tttatagact ttttaaacac agggaatgga gtcgaagctc caacagctcc
    12361 tatagttcca ccaaacacaa caagtgctga tgaaaataca aataatgctc caacatgtcc
    12421 cccatcaata acaaaagcaa ctaccaaaga tgctatcccc actatgaaaa aaattaaagt
    12481 tgtcatctat tccctccttt ctttagttat acatatctaa tcatttatga cattataaaa
    12541 agactatctt taataaaaat agcctttaat aattggcgga gaaggaggga ttcgaaccct
    12601 cgcaccggtt acccagccta atcccttagc aggggatcct cttgagccac ttgagtactt
    12661 ctccatatct ctatactagt aaattatata ataaaatcat attttagtca attgttttat
    12721 aaaatttgag ttattttgaa ttattaaaca tctattttta cttttttgct taaatgtaag
    12781 ttttaaatat ggtaaaatta tcttgcataa cacttataat attatttgat aaatatacat
    12841 acattaacaa atatacctaa actctgactt aattgtaagt tatgccaaaa ctaaaataat
    12901 actagtccaa ggaggatatt ttatgttacc acaggaaaga tatgagaaga ttttaagtat
    12961 tctcaaatca gataatataa ttaaaataaa tgacatagtt caaatgtttg atatatcaat
    13021 agaaactgca cgaagagatt tagcccatat ggagagtatc ggtcttttaa aaagagttta
    13081 tggtggtgct ataccagtta tagcaagtag cgtagaactt aactatgtta aaagaaaaaa
    13141 aataaactca aacgataaaa aacagattgc tattaaatgt agtgagttta taaatacaaa
    13201 tgatactatt tttatggata ctggaactac tgtacttgaa gtttcaaaaa atttgaaaga
    13261 taaaaagaat ctaactgtaa taaccaactc aattttagtt gcaaacgaac ttatgaatac
    13321 taatataaaa gtatatgtgc ttgggggcta tcttagagaa actgaaggtt ctttttctgg
    13381 tccgcttaca ctgtcagcta ttgaacaatt taatgttgat aaagctattt taggcgctgg
    13441 aggtatcact cttgaaaatg gaatctctga ctatcactta gaagaagcac ttgttagaaa
    13501 aaaaatgatt gaaaggtcta gaaaatctat agtagttgtc gaacctccta agtttggtat
    13561 aaattcattt gcatcagtag ctcctataga acatatagat actattatta cctctgctag
    13621 tatagaccat aatataataa tagagttttc tcaaaaagga atacctttag tcatagctga
    13681 taattagctt atatgattga tttttagttc cttgatgtaa taaattaaaa ttaaggagcc
    13741 aaataattat ttatttagct ccatttatgt ctaaactata ctttatattt taattatgta
    13801 actactttca gttagcttat catttttagt tatttgattg ccattttatt taatttataa
    13861 gttttagtta ttcgattatt ttttaattgt ctgtcttttt aatttatttt tagttattta
    13921 aactcctact ttaaaataat ttaaatttga cttaagtatt gtttattcta cactgcatct
    13981 ttcttcattt taatactttg ttcttcttcc aaggttaaat cttttgtcat aagtatagca
    14041 agtgcaattg ccaatactcc tccaaccaat tttccaatta caacaggtgt aatcatctct
    14101 ggaactactc ctgctgtaaa tcctaaatgg tcccccaaag ctgctgtagc acaaaccagc
    14161 caagcagtat ttactacttt cccctttgga ctcatatctt tcatcatttt atatactgga
    14221 atactattgg ctaaagtaaa aactaatcct gctgcactag tggcatccat acctaatttt
    14281 ccacccacta ttgtaagtgg tttattaaga actttaacta ataaatgtaa tattggaaaa
    14341 gtacctaata acacgattgc aatacttcca attacttgaa taccttctct tataggtgtc
    14401 atgcctttta taagtactat acctgttatt tcttgaaatg ctgctgctcc caaacctatc
    14461 gttatcaata tagatataaa ttttccaaaa attaatgacc catttatcat aagcgtagaa
    14521 ttaaatttta aacctactgc caatatcact gccaatatta tcactggtat catatttata
    14581 attaccattg ataagttaaa tcctgctatt aatcctccta taatacctcc aaaaggtatt
    14641 gttatcaagc ctatcagtaa cccttttgca aagaatggtc tgtcttcata ttctataaga
    14701 cctagtccta ctggaattga aaatactaat gtacatccaa gcatggatga cacaaccaat
    14761 cctgcaagca ttcctgcctc tttattgtgt gcaagttcca tagccaatgg ataccctccc
    14821 atatcattcg ctaaaatagt tgcaaacata gatgggtcag ctccacagag attgaagatt
    14881 ggtattatta taggacctag tacatctgaa attactggag ctaaacaaac tataccaacc
    14941 attcctaaag ccaatggacc cattgaatta aacccttctt caaactgctc tccaagtcca
    15001 aatttatttc caattattct atctaacgct cctaaaagca ctcccacagc cattatgtat
    15061 aatataattt tatcaaaggg catttttaat tcctcctata catgtaaaat actctattgt
    15121 aacttaatta gcacacacct ttttggtatc gttttcccaa ttcatagctc tttttactgc
    15181 ttgattccat ccaagtaata tttcttctct tttttgatta gtcatatttg gaatatattt
    15241 tttgcttatt gtaaattcac ttctaatttc atcaatactg ttccagacac cacaagaaag
    15301 acctgccata tatcctactc caagtactgt cgtctcagga ttttctgcaa tttcaattgg
    15361 tatacctaat atgtcggatt gaaattgcat tgtaaagtta tttttagtaa ttccaccatc
    15421 tactctcaac atttttattt ttttccctga ggctaagttc atagaatcaa caacatcttt
    15481 gacttgaagt gcaacagact caagagtcgc tcttactata tgctcttttt tagtacttcc
    15541 tgttatccca accattgtac ctctagcata ttggtcccaa taaggtgctg ctatacctgc
    15601 aaatgctggt acaaaatata cacctccagt acttgatatg gaatttgcca tgtcatcagt
    15661 ttctgaataa ttttttataa ctccaatgcc atctctaagc cattgtattg cagcacctgc
    15721 tatatacact ccaccatcat aagcatatgt aactttatct cctattttcc aagcaacagt
    15781 tgtcaaaata ccattacctg ctatatttag ttcctcacct atattcatat ataggaaaca
    15841 tcctgtacca taagtatttt taaccattcc tggttcaaaa catccattgc caaataaagc
    15901 tgctggttgg tctaccattg aagcagtaat ttttatagaa accccacaaa atgcctcctt
    15961 atctgtagtt ccaaaatcac cactcgtagg cattatatct gctaaaatac ttctaggtat
    16021 tcctataatg tcaagcaact ctctatccca atccatatct cttatattaa aaagcattgt
    16081 tcttgctgct gtagatacat cagttaaaaa agattttcct cctgtcattt tccatattaa
    16141 ccaactatct aatgttcctg caagtacttc tccattttta attttttctt ttacaccttt
    16201 tacattatct attacccatt taatttttgt acctgagaaa tatgggtcta taattaaccc
    16261 agtcttatct attatcttat tttctaaacc tggtatattc tttaaatttt ccacataatc
    16321 ataagttctc ctgcactgcc acacaatagc attgtaaagt ggtttacctg tatttttatc
    16381 ccataacatt actgtttcac cttgattatc tataccaatt ccttttatat cactttcatt
    16441 gattccattt tcaaaagcct gttgtaatac attattactc acagtttttg tattattcca
    16501 aatttctata gggtcatgtt caacatatcc agattgtgga tatatttgag tatgttccat
    16561 ataatcgcta aaaacaaact ctgatttttc gtttatcaat actgctcttg ttcctgttgt
    16621 tccttggtca attgctaata catatttcac tttaaatgtc cccctttatt ttcagaatta
    16681 acaaattctt ttttattttt tgaatatata atctattcaa gttatttata ttttatatca
    16741 tcatgtggat ttatgcaata ttcattacaa tattttttgt tatttttaat tatagtgatt
    16801 aaaaaagtat ttttattgaa atattattgc aatatttatt attttattct ataatctagt
    16861 taaaataaca cataacattt taatatttac acataatcag tcattcaaaa atttaaattt
    16921 aactttttaa ggaggcttta caatgaaaaa aacatatcta ttcagtcctg gaccagtaat
    16981 ggttacagat aaagtcagaa attcacttct tcactatgat atatgccata gaggaaatga
    17041 gtttatgaat ttattcaaag atacacaaca aaaaatcaat aaactatata atgcaacttc
    17101 taattactac tcattaattg tatctggctc tggaacatct gtaaatgaat gtgtactatc
    17161 ttctatcttt gacaaagaag atgcagttct gctagttagt aatggtgttt ttggagaaag
    17221 gctagaagaa atattatcta cttataatgt aaaaacatat aaacctaatt ttgaatgggc
    17281 agaatacccg gacctagaca ttttagaaaa atacataatt gaaaattctg atataaaagt
    17341 aattgctatg gtgttccatg aaactagttc aggaatgata aatcccgtcc ctgaactagg
    17401 aaaacttgct aagaagtata acaaaatatt ttttgtagat gcaataagtg cctcagcagg
    17461 tgaatatatt gatgtggaag agtttaatat agatataata acaggtgttg gtggcaaagc
    17521 tcttggagca tttccaggtt ctgcatacct ttgtgctaaa gagagtattt tacaaaatat
    17581 taaagaaaat caatgtaaga atgtgtatct taatctttat aaacactata aagttgctaa
    17641 atcatcttct caaacaccaa atacacctaa tgttacttta atatttgccc ttaatgaagc
    17701 tttaactgaa tttttggaag atgacagtaa aatagaaaga tacaaagaat gttctgctat
    17761 tttaaggaac ggtatggaag aattgggtct aacattttta cttccagata agtatatgtc
    17821 taatacagta acatctgtat ttctacctaa agaaatcaat gtaaatgagt ttatattagg
    17881 gctagaaagg gaaagtggct atgttgtcta tccaggaaaa ggcaaattcc tagatgctaa
    17941 catattccaa gtagcaaata tgggagaaat atatccagac gattgttata aattcttaga
    18001 tatcttaaaa tcaaaattgg agataaaaaa agttatataa cagtttgttt gtaaaatcct
    18061 acaatatatc agtataaatt aaatagactg atttttttaa attttcctat aataaattat
    18121 attttatatt tttgtataag ggtgaactat aatatgtaaa gttcacccct atttcaaatc
    18181 tttttccttc aatataatct ttttctttta atttatttta tatttttgac gcaatcgttt
    18241 tttattcaat cattttatat tcttgataca atcattctta cacttaatta ttttttgttt
    18301 ttgacacaat cgttttttgt tgaatcattt tatattcttg atacaatcat tcttacactt
    18361 aattattttt tgtttttgac acaatcgttt tttattcagt catttaatat tttcaacaca
    18421 atcattctta cattctatta ttgtttgttt ttgatacaac tgtttttatt cagtcattta
    18481 atattctcaa cacaattacc ttttcattca atcaaagtaa tctttaagca ttatttttca
    18541 agctcatcaa agtaattata tacgataacc tcacctgtct ctttatctgt agtctttata
    18601 atgcccttag ctccatttac ctcttctttt gtaaattcat catctaacac aactttgaat
    18661 ttatcttcct ttccataacc ttgttgaaat tcatctctca aaattgcatt tttttctgct
    18721 tttggcttca actcaaattc aattccagat ttcatttcat caaaataatt taatactaca
    18781 acctcattat tcattttatt agttgtcttt ataatccctt tagctccatt tacttcttct
    18841 tttgtaaatt catcatccaa tgttatatta aatttgtctt cttttccata ttcctcttga
    18901 aaatccctct tcatttcatt gtatttagtt tcaaactctt tactcttgtt taattccata
    18961 gctgatacat ttgtcaatga tgctccaatt gccattactg ttaatgccat tactatacca
    19021 cttttaaatt tttttacttt catatcttac ctcctaaatt aaatgttctc aatgaaatta
    19081 tataaagaaa atcctaagtt ttacagtttc attcttgtaa ctaaaattac tattatgtaa
    19141 ccaaactttt tgcttcaact attccttaaa agttgctttt ttgaggaata gttgaagtta
    19201 aataattaga ttttatctat atttatagat ttaaaatcaa atttatccat ataaataaat
    19261 acttattttt gcaaaataaa aatttttaat tactccaatc cttcttattt acttaaaatt
    19321 ctatgataag aaatttaaaa gtctttacct tctttaacaa taatttattt acttttaaca
    19381 tacaaaatag agagagtata tcaaaatcat tttactga
//
LOCUS       DAELNY010000063        19353 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.063, whole genome shotgun sequence.
ACCESSION   DAELNY010000063 DAELNY010000000
VERSION     DAELNY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.063"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..441
                     /locus_tag="KPE55_001878"
     CDS             28..441
                     /locus_tag="KPE55_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492962.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            632..1849
                     /locus_tag="KPE55_001879"
     CDS             632..1849
                     /locus_tag="KPE55_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG0492963.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            2011..2709
                     /locus_tag="KPE55_001880"
     CDS             2011..2709
                     /locus_tag="KPE55_001880"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG0492964.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(2929..3546)
                     /locus_tag="KPE55_001881"
     CDS             complement(2929..3546)
                     /locus_tag="KPE55_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG0492965.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIESFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(3673..4695)
                     /gene="argF"
                     /locus_tag="KPE55_001882"
     CDS             complement(3673..4695)
                     /gene="argF"
                     /locus_tag="KPE55_001882"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG0492966.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            complement(4799..5986)
                     /locus_tag="KPE55_001883"
     CDS             complement(4799..5986)
                     /locus_tag="KPE55_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG0492967.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLCSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKNI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(6016..6876)
                     /gene="argB"
                     /locus_tag="KPE55_001884"
     CDS             complement(6016..6876)
                     /gene="argB"
                     /locus_tag="KPE55_001884"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG0492968.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(6891..8108)
                     /gene="argJ"
                     /locus_tag="KPE55_001885"
     CDS             complement(6891..8108)
                     /gene="argJ"
                     /locus_tag="KPE55_001885"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG0492969.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(8149..9183)
                     /locus_tag="KPE55_001886"
     CDS             complement(8149..9183)
                     /locus_tag="KPE55_001886"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG0492970.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGSAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(9437..9650)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9953..11044)
                     /gene="ytvI"
                     /locus_tag="KPE55_001887"
     CDS             complement(9953..11044)
                     /gene="ytvI"
                     /locus_tag="KPE55_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0492971.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFVIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     gene            complement(11059..12450)
                     /locus_tag="KPE55_001888"
     CDS             complement(11059..12450)
                     /locus_tag="KPE55_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBG0492972.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFANYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVESRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            complement(12469..13008)
                     /locus_tag="KPE55_001889"
     CDS             complement(12469..13008)
                     /locus_tag="KPE55_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0492973.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLMIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            complement(13029..14354)
                     /locus_tag="KPE55_001890"
     CDS             complement(13029..14354)
                     /locus_tag="KPE55_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0492974.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            complement(14351..14974)
                     /locus_tag="KPE55_001891"
     CDS             complement(14351..14974)
                     /locus_tag="KPE55_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBG0492975.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            15066..15875
                     /locus_tag="KPE55_001892"
     CDS             15066..15875
                     /locus_tag="KPE55_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0492976.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            16309..16671
                     /locus_tag="KPE55_001893"
     CDS             16309..16671
                     /locus_tag="KPE55_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0492977.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(17021..17578)
                     /locus_tag="KPE55_001894"
     CDS             complement(17021..17578)
                     /locus_tag="KPE55_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0492978.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            17836..18534
                     /locus_tag="KPE55_001895"
     CDS             17836..18534
                     /locus_tag="KPE55_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0492979.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         6745 a         3193 c         2262 g         7153 t
ORIGIN      
        1 aagattatac aaggagaaag ttcaaatatg gaattagaaa ttacagaaaa aatagaaaaa
       61 caagatgaag atactatttt tcaaggatta ttaaaatata atcttgctag acttgaagat
      121 aaaaacccag tggatttagg aatatatatt cgagataaag caggtaaaat tgttgcaggt
      181 tttataggtg ttactcatgg taattggctt tctataaaat acttatgggt tagtgagaag
      241 cttagatata aaggtactgg aagtcaactt ttatataagg ctgaaaaaat tgctaaagaa
      301 cgaggttgta aatatgtttt tttagataca ttcagttttc aagcacctaa attttatgag
      361 aaatttggat accataaagt attttcactt gaaaattatc ctctaactgg aaagagatat
      421 tattatagaa aaatactata ataataaaaa tcatatttca tatttttaaa ttaaaacttc
      481 tgctcttcgt ctacagactt tatgttttta tatcattatt aatactaaaa tagtatttta
      541 ctatacatac aaaattattt gaaagttcaa aaattataag tataaaagct aatcttagct
      601 taaataaaaa ttatattttt aggaggtaaa tatgttagga aaaaaatgta tggattatct
      661 acaaacatta ggaaaaataa gttcaacaac aaatggtctt acaagactta tcttgactca
      721 agaacataaa aaatctattg atttaattag ttcatggatg gaaggtttaa atcttgatat
      781 tgaaattgat gacattggaa acgttatagg tacatataaa tcaagctttc caaatgctcc
      841 cacattagta gttgcatctc atcaagatag tgtaaagtgt ggaggtatat ttgatggtat
      901 gttaggtata atagtgcctc tagttggtct tgaagaagca aaacacaata ataggtctta
      961 tcctttcaat attaagctaa ttgctttcgc tgaagaggaa ggaactagat ttgaaacttc
     1021 tttaatggga agtaaagttt ttgctggtac ttttaaagag gaacttctta aatctgttga
     1081 tgaaaatgga attacacttg aagaagctgt aacaaagttt ggatttaata caaaaaactt
     1141 aacaaatttg catcctagaa aagatgtaga tgcatattta gaatttcata tcgagcaggg
     1201 tcctgtttta gaaaatgaat ctcttcctgc tggaattgta tcaagtatta caggatttaa
     1261 aagttttaag atatcagtaa atggtaagtc tggacatgct ggtacacttc caatgaatat
     1321 gagacttgat gctggatgtt gtgcctgtga atgtgtatta gctatcgaaa aagtagctaa
     1381 aacgactact gatttggttg ctactgtagg aaagatgaat ttctatccat cttcatcaaa
     1441 tgtggtacct gaaagagctg aatttactct tgatgtacgc tcttgttcac aagaaattct
     1501 tgataattct gtagaaaaga tatttaatga aatatcacat atatgtgaaa atagaaaact
     1561 taattataca agtgaacttg cttttgaaaa tgttcctgta ccatgttcta ataaaataac
     1621 aaaaatcata gaaaaaagtt ttatagattt aaatcttaat cctttttata tttatagtgg
     1681 tgctggccat gatgctcagg aaatggataa tataactgat attggtatgg tatttataag
     1741 atgcgctggg ggcgtaagtc ataatcctaa tgaaagtgtt tctgtagatg acttggatac
     1801 tgctgtaaaa atatttttaa aaatactaga taatcttgat ttaaaataat tcttatataa
     1861 gaaagtatat taaaatatat aatctaaaaa atcactttat atttatatta tataagtgta
     1921 caatataatc tattataaaa tataatttta tgcttttata gatttttatt ttatacataa
     1981 atatatttat atataaagga gataattttt atgaatgaaa aattaattgg tattttggct
     2041 ggaatgggtc caaggtcaac agcccctttt gttgatttaa ttattgatga gtgtcaatct
     2101 caatatggag caaaatatga tgatgaattt ccaaagatga tgatatattc attacctacc
     2161 ccattttata ttgaccgtcc aataaatcat gaacttatga aagaaactat aattgatgga
     2221 ttacaaaagc ttgaatcaac tggtgtaaat tttattgcaa tgccatgcaa ttcagcacat
     2281 atatacttta aagaattaaa agagtctatt aatataccaa tattaaatat tgtagaagaa
     2341 acagttaaaa aattacctac tatctcacaa aaagtaactc ttttttcgac tagttcaact
     2401 tttgagtcta caatttacca gaatggtata atacataatg gacatgaatt tatatttaaa
     2461 gatgaatggc aaataaaatt aaacaatctt attcaaaata taaaaatgga taaagaaaat
     2521 ccacacaata ttgatatttg gaataaactt atcgaagatg ttaaaaatga atctatagaa
     2581 aatatagtaa ttgcttgtac tgatttaaat gtagttttag aaaaatcata tccttcaata
     2641 aatattgttg attcatcaaa atgcttggct gaatcagttg tcaataaata tctaaaatta
     2701 gttaaataaa tttttcatgc actatattat aaaaaatata tgttataagt ttatcttaaa
     2761 tagtataggc ttataacata tatcatagtt tcttgacaat atagaaatta accatgtatt
     2821 tcattatatt aataaaaagg ataccaaaaa tggtatcctt cataaatatt tatatgttta
     2881 tttataaaaa taatcatata tttattactt tactacacct aaacaatatt attcaagtgt
     2941 attcatagag ttagaaaatt cttgtcctgc tgtaacaaga ttagataaca atacatcagc
     3001 tactgcatca tttactgaac ttattatcca tttattatcc tttttagaac attcaaactc
     3061 tacatttttt gtaacataag tctctttaac gcttttttgt ttttctttca taattgatac
     3121 taacatttca gttgttttct catcagttaa gtcttgtcct gaaaaactca taccaatcat
     3181 ctttgtaaat gcctcagcta ctatttcttg aagtaatggt gttgaatcta taaatttaca
     3241 ttctactttg attgtagcct tatcaccttt aacctcgctg tcttttattg tatatgtgat
     3301 tttagatgca ttctgtttta gataatctaa aacatattgg gcatttgggt cttttgtatc
     3361 taattccttt aatatatcct tatatttttc gtcattattt tccattacct tattcatgcc
     3421 ttcaaagtca aactttttcg cactgttaaa aaaactctca attgtatctt ctggttttgc
     3481 acttgaacat gctgttaaag atattgctaa taaaaaagtt gttaaagcta atatgatttt
     3541 tttcatatct caaatcctcc ctcttaattt gttgatacat aagttatttt aacatataat
     3601 gagagttttt gaataatata tttaaaatat tttataaatt ttcattaaag taaagtctat
     3661 attaaaacat atctatacta aattattgtt catatttaca tcggtcatag tagaaaccat
     3721 aacagctttt attgtatgca ttctattttc agcttcttca aatacctttg aatattttga
     3781 attaaatacc tcgtttgtaa cctctaattc tgataatccg aacttttcat aaatctcctg
     3841 agcaacctta gtttctaaat catgaaatgc tggaagacag tgtaaaaata taacattttc
     3901 attttcagtt ttctttatca tatccatatt tacttgataa tctttaagtt gatttattct
     3961 tgcttcatat tgctcctctt ctcccataga aacccataca tctgtatata taacatctgc
     4021 acctttaaca tcatcaatat tttcagtcaa cattatatct cctttggatt tcttggctat
     4081 ttccttcatt tccttaacca attcttcatt tggccaaagt tccttaggtg ctagtgctac
     4141 gaaatgtatt cccattttag cacatcctat catcagtgag tttcccatat tatttctagc
     4201 atcaccaaca tatacaaatt taactttatt caaatcttta tctacatttt caattactgt
     4261 taaaaaatct gccagtattt gtgttggatg atattcatct gtaagtccat tccaaacagg
     4321 tacaccagaa tattttgcta gactttcaac tgtttcttgt ttaaagcctc taaattctat
     4381 tccatcatag aatcttccta gaacttttgc tgtatctgat acagactctt ttttacccat
     4441 atgacaatca tttggaccta gataagttac atgtgctcct tcatcatgag ctgctacttc
     4501 aaatgaacat ctagttcttg ttgatgtttt ctcaaataat aaagctatat tttttccttg
     4561 tagcaaatcg ccttttatac caactttttt cttagttttt aaataacttg ataaagttaa
     4621 taaatatcta atctcttctt ttgtaaaatc ctttaatgtc aaaaaactct taccttttaa
     4681 acttatatta ttcatattaa ttcgcccctt cttataaact ttttaaattc aattaaactt
     4741 gttagcactt tactttttta tttacttcta attttttaat taactatttt ttgaatattt
     4801 agttaatttc tagtaatata tcttttagga tttcaagagc ctcatctatt tccttataac
     4861 tgattgttaa tggtggtaaa aatctcaata cattttttcc agcagtcaaa ataagcaatc
     4921 ctttttttct agctttctct tctataatac tagattcaat attagtttca attcctatca
     4981 ttagaccaat accttttgtc tttaatacaa ctttgttatt aaaactttca atagattttc
     5041 ttatataatt tcctttttct aatacttcat tataaaaacc atctttagat atctcatcta
     5101 atacaactga agctgccacc atagctaata aattacctcc aaaagttgta ccttggtctc
     5161 cttttcctag tacttttgaa agttttttat ttaccaatac tcctcctact ggtattcctg
     5221 ctcccaaccc ttttgctacg gtaactatat ctggttttac atcaaaatat tcatagccaa
     5281 aaagtttacc agttcttcct ataccgcatt ggacttcatc aaaaatcact acaatattct
     5341 tttcttgaca tatttttact atttccaaaa cataatcttt ttcaagaaca ttaactccac
     5401 cttcgccttg aatcgcttca agaattattg cacaaactgt ggaatcaaga ttatttttta
     5461 aatcttcaat attatttctt tctatatatt taaaaccttc gggaagagga taaaaatact
     5521 tatggtattt gtccatacca gttgccatta aagacatcat tgttctacca tgaaaagagt
     5581 cttttaaact tatgatagtt cctctaccat taccatattt gtcactacta tactttcttg
     5641 ctattttgaa tgctgtttca tttgcttctg ttccagaatt acaaaaatat actttagcca
     5701 ttccacttga attaattatt ttctctcctg aacacaacac tggctcatta aaatataaat
     5761 ttgaagtatg taataattta tctacttgct cttttagagc attctttaaa ttactattac
     5821 tatacccaag agaagatact ccatatccac ttgtaaaatc tatgtattta ttgccattag
     5881 tatcaaaaaa acaacttcct tcaccataat ctatgacaaa attgggttgg ttatatgtat
     5941 ctataaaata ttcattccat tttgaaattg tattgtcgtt attcattctt attctcccct
     6001 cttaaactta taattttatt cattttcttt tcttattaat gtacctattc cactatcagt
     6061 aaatagctct gttattatag aatgtggaac tcttccatcc aaaatatgta ccctatttac
     6121 tccgttattt aaggcatcta cgcatccctc tattttaggt atcatacctc cagtaattat
     6181 accttcttca aataatttat caacatcctc taatataact tctgttatta atgatttttc
     6241 ttcatctggc tctcttaaca aaccagggac atctgtaaga agtatcaatt tatctgcatt
     6301 taactcttta gctatagcag aagctgctgt gtcaccattt atattataag tctctccatc
     6361 atctccaaca cctatagttg caattactga aatataacct gactttagac aagattctat
     6421 aagctcaaca tttacttttt tgatttctcc tacaaatcca agttcataat ttttatatgg
     6481 gtcacataaa atcatattat tatctattcc acaaagccct actgcttttc caccttttgt
     6541 atgtatttta tttactaggt ctttattaac ctttccagca agtaccattt ttacaatttc
     6601 catggtctct tcatctgtat atctaagacc atttacaaat ttactttcta tatccacttt
     6661 tgctagcatc ttattgattt ctgcccctcc accatgtact aaaactatat tgattccaac
     6721 ataactcatt aacacaaggt cttccataac agattctttt agaccatctt ttttcattgc
     6781 acttccacca tacttaacta caatagtctt tccctgatgt ttttctatat atggtaaagc
     6841 ttctataagt gtatttgctt tttctatatt aatcattaaa gtatatcctc ctaatttcta
     6901 taacttccat ttattcttac ataatcatag cttaaatcac atccccatgc atttcctctg
     6961 gcattgccca tatttaaatc tactaatata cttatttcgt catcttcaag gatttttttt
     7021 gctttttcct cattaaaatc taatccattt ccatttttac atacttcaat atatcctttc
     7081 atactctcaa aaataatatc aactttttcc atatctattt cttcacctgc ataacctaaa
     7141 gcacacagta ttctacccca atttgcgtca gctccaaata cagctgtctt tactaatgaa
     7201 gaagatataa ctgtttttgc taattttact gcatgttctt cacttaatgc tccatttata
     7261 tagcattcaa ttagttttgt acatccttct ccatcttttg caacaagttt agcaagttct
     7321 atacagacat atgtaagtgc actcaaaaat acttgataat gttcatcttt tttgtttatt
     7381 ctatcatttt ttgcttttcc atttgctaga atcattacca tatcattagt acttgtatct
     7441 ccatctacac taactctatt aaaacttttg tcaacagcaa tttttaaagc ttcttttagc
     7501 aaaactccat caatatttgc atctgtagtt ataaatccta acgttgttgc catattagga
     7561 tgtatcatgc cagaaccttt tgccatacct ccaatagtta tttttttatc tccatacatt
     7621 attgctactg ctatttcttt ttttataata tcagttgtca taatagcttc tcttgctcca
     7681 atatgacctt gtttacttag attattcact aattcatcca tattttcttc tattaaatct
     7741 atatttaatg gctttcctat aacacctgta gatgcaacca aaacatcact ttctttaata
     7801 tttaatttat cactggtgaa tttagccatt ttataagcat ttataagtcc ttcttttcca
     7861 ttaaaggtat tagcattagc actatttact atgatagctt gtgcctttcc atttttaatg
     7921 tgttcctgtg taacataaac aggattgccc tttactttat tttgagttac tactgctgct
     7981 gcatcacata aaacatctga ataaaccaaa gctaaatctc ttttttcttt attttttctc
     8041 aagccacagt gaatccctga agcaaaaaat ccttctgaaa ctgtaactcc accctttata
     8101 atctccatat ttatcctcct aattatatga gtttagtatt ttttatttct aaaatgatgg
     8161 tgcaatattt tttaaacctg tattttcttc aataccaaat attatattca tgttttgtat
     8221 agcttgacct gctgatccct taatcatatt atctatagct gaacagatta tcaatgtatc
     8281 tccattttca tgtaatgata ttacacattt atttgagaat ctaacattct ttaaagaagc
     8341 taccttatct aatggaagaa tttctataaa ctctttaaat tcataataat cagttaattt
     8401 cctatgtata tcatttatag aaatattatc ttcttttgta caatatatag ttgataagat
     8461 tcccctattt actgggatta agtttggtgt aaaaactaca cttacttttt ctttgcagga
     8521 ttcacttaaa ttttgttcta tttctggagt atgtctgtgt ttccctattt tatatgcagt
     8581 gatattttca ttaacctccg taaagtgact actaatcgat aattctctac cagcacctgt
     8641 aagacctgat ttagaatcaa ttattatatt attatttttt atcaatttag aacttagtaa
     8701 tggcatcaat ggtaaagaaa tactagttgg ataacatcca ggatttgcaa ttatatctgc
     8761 atcttttata tcttctttgt atatttcact taatccatat acagcttttt tatgtaagat
     8821 tttatctata aaagatactc cataccattt actatatact tcttcatctt ttattctaaa
     8881 gtcagcacct aaatctataa ctttcttttt cgatttaatt gcctttatta caaacttttc
     8941 actaactcca tgtggaaggg ctgtaaacac aacatcacac atatctataa ctttttcatt
     9001 ttctatacat atcatgttat ttttatatcc tatacttgga tatatatcaa ctatatcttt
     9061 tccaacatac gaatttgaac ctatcgctgt cacttcgact ttatcatgat tcattaataa
     9121 tctaatcaac tctgccccga catatccagt tgcaccaata ataccaactt ttattttatt
     9181 cattttgttc cctccatttt tatattcaaa tagtcttaaa atcctatctt tccactttac
     9241 tatatgataa tcatttgata atacgtagat taaaatactc taattaaaac aaaaaacccc
     9301 ttagcattat aaatactaag aggcgatttt ccgcggtacc actcttattg gtaaaattac
     9361 aataatcatt aaaactcaaa ttaaaaagta tctacaaaat gtacatttaa aattaaaaaa
     9421 gcctcttggt caaagaccaa gagacgaata tacacccgcg ttaccactct aattgataag
     9481 ataatcttac ccactctaat ctcttaaggc gagattcacc aattaccata ctattacttc
     9541 tggtaatctc ctccaaagct ctattcacaa taaaattcgt tactaggctt ccaccattcc
     9601 cagctctcta tcaactagct ttatgctact ctctttttca caggatttca atttattttc
     9661 ttattgtttt atatgatagt ttattttttt aactttgtca atataatttt taagtttttg
     9721 gaaatatttt ctatataatt aatttccagc attttgtatc taaattgagt tataaatatc
     9781 tacagtaata ggacaccatg aatttcctgc tgtaatatat tttaaaaaag ttctccctct
     9841 tgaatcaagt cattcaaaag ctataccttt gtcaaaatta tttttcatcc agcgtttttt
     9901 agaaaaaaca caattttctc cataaaagag acatacatac aactattctt tcctaatgta
     9961 atatatgtat gatttctttg tcatttaact ttttcaaaaa cgcaattaac aatggaatac
    10021 cgaacaaccc aataaatcca aagaaatgta atcctgttag catactggct aaggccaata
    10081 caggatgtaa ctccatatgt ttaccaacta attttggttc aataatattc ctgataatag
    10141 taatcacaat atatagtagt gcaattccta atgccattga ataatcccct aaaaataaac
    10201 tgataataca ccaaggtatt agaatacttc cagttcctaa aacaggtagt atatctaaaa
    10261 gagcaatgaa taaagcaatt attccagcat aagaaatatt aataataaca aaaccgacat
    10321 ataattcaga aaaagtgata gcaaaaatta aaagataaga aaaaccacat ttaataatgg
    10381 tatcaatagt aaacgctttt gcttctgcta aaagataact tttattttta ggtatctgta
    10441 gcactataaa tctctttatc gttggataat caattgctat aaaaatagaa gcaacgattg
    10501 taataattgt tttcataaat agtaatggaa cagacgaaac aatatcagta ataccagata
    10561 cgatattatt tgaaagagaa actatgaaaa cactcatttc attcatcaat acaggcatat
    10621 ttgcttctat taattcaata aacgaaaaat tgaatgaacc agaaaatagt tcgacttctt
    10681 ttataactgt ttcagtcaaa ggtattacca attcctcaaa catggttggt aggaaatgaa
    10741 aaatttcctt tattccatat attagatatg tacctattac acttactatt attcctgcaa
    10801 ttaaaaagaa taaaaaaaca gatatggttg ctacaatacc tcgctttatt agtaattttt
    10861 cacttatgta taagactggt ttattcaata aacttgctat taaaaatgca aaaacaaaag
    10921 gaagtaatac agaaaacgca tatttcccta ataacattag taagcctaat atcactacca
    10981 aataacagaa atttattaaa aatctctgct tattttggta tgatgtatga tcttgttcat
    11041 tcaaataaaa tgcacctcct actttatagt aactttatat gtgccttttg gagcaacagg
    11101 gggcattgtt tcacacattc cagaacaact ttttgcttgc acaaaatatt caatcatatc
    11161 acctgacttt cctatcatat tggcaaaaaa tatttctgct tctgtagttt cttctaacct
    11221 aatactttcc catgtttgaa aaccatagta tcgataaaac agtctacaat tttcataatc
    11281 taagccagct ttactataat caactatttg aatagtaact tctaaagcat cattctctga
    11341 aacgatttct tctaatcttt tcacggaaat atagagcata ttctcatccc aaattgctct
    11401 tgtcctgcaa tgaataacat cttcatttcc ccagccagtt ctcacatatc ctgtacgatt
    11461 ttttataata tctggctctt tttcaaaatc aaactcatat cctttaattt catatccagg
    11521 caaagcgttc tgccattgtt ttattgcaat tttatcctgt tcaattccat acattggaac
    11581 atagacacga ctatttaaaa tcacagaatt aacataagca gtaagttcat cactgtcctt
    11641 ttgaaacgga acaatatcta tacgaaagat tttgaatgga cgtccatatc tgctttctac
    11701 cttttttact tcttctaaaa ttttttcata tctttcatac aaatagtgtt cttttggcgt
    11761 tctgctcaca agtaatgttt cttcatcaat tatttttaaa taacagtcaa tatgttgtaa
    11821 accataatta gcaaaattag aaaatatata atactcattc attccagtat ctcttgccac
    11881 catagaaaaa aattcttcgt cagaaattcc tttcgcacga ttttctgtct gtaaaatgtg
    11941 catagacatc aatttattga aaccatctga cataagattt ccgcctgtta gtgcataagg
    12001 taatttcata aatggcaaac caatctgttt cgcaatcaac tcttgtgatt gatcttcttt
    12061 ttgttcataa gagttagtag ttaataggtt atgttcgcaa tcaaacatga tttctggttt
    12121 atttatggat acaaatggtg ttgacatctt atattctgca ccagccaaaa agagttttcc
    12181 gttttcatca aaaatagggt gcatacccca atcacgaacc catggtgtat caaatccctg
    12241 tggagcaatg cagaaaatca aattttttaa atttccaccc cacttactta aataatattt
    12301 tgcaatttca atttcctctt tatttggtac acacatgtat acagaataat tttgcgttaa
    12361 atctacaata tacttatggg gaagaaaagg tggccatgca agcatgacac ctttaatcgg
    12421 ttcccattcc ccagctaaac gtcttttcat ttctaatacc tcactttctt actttttcca
    12481 caaatgaaat ggaatttctc taattttaag atttttcaac aatgtttcaa attcattttt
    12541 ttgaaaggtt ttctgattac ataaaacttc catttctagt cccttatact ccatgagata
    12601 ttctgtttca taaaacccat tctgaagata aaattgcttc cttaaaattc tttgctgtaa
    12661 attaggagca ctgtcctcta tagtttccat atcaagaaca atttgacaat tgggatattg
    12721 ttttttcata agtgacagtg cttttccacc atatccttta gagcgttgtt ctggggaaat
    12781 tgcaagaaat aatatatatc ctatatgatt ttcaatcatc aaaacataaa aaccaataaa
    12841 ttgttgctta tcatataaag cagatacttc tattttttta ctttgaacta aattaagcat
    12901 ttcttgtata gacatacgtt cttcatctgg aaatgcctcg ttattaagtt cttcaaattt
    12961 ttctaaatct ttaaaatatg aagttacttt ttgtattgtt aagctcatat agatacctcc
    13021 aaattgattt aataatgata ttttttctgt tttcttatta taaagaaagc cgtcataata
    13081 gttgccaata tttcagcacc aacaatagaa aaccatatcc catttactcc ccagaatata
    13141 gggaatataa gtactgatac aacttgaaat actaaaatac gcataaaagc aataagcgca
    13201 gaagttaaac cgtcatttaa tgcagtaaag aaactagaac caaaaacacc aaaaccagaa
    13261 aataaaaagg aaaaagagta aatcataaat gctcttaatg tcatttccat taaattatta
    13321 tcgtaaccca caaaaataaa agcaatcggt tttgataaaa attcgcctat tataaacata
    13381 aatacagcaa aacaaataat tacaatcaaa cttctttttc ttaaattttt taattcgtca
    13441 taattttttg ctccataatg ataactgatt ataggggcag taccaacaga atacccaata
    13501 aaaacagctt gaaatagtaa cgctacataa agaagaacac cataggatgc aacgccatct
    13561 tcgccagcat attttagtaa ttgagcatta tacagcatac tcacaacaga tgtggaaaca
    13621 ttagtcatta gttctgaaga accatttgca acagtctttt tcaaaacatc acaatcaaat
    13681 ttaggtttct taaatttaag caaactgttg ttttgtcggc taaaataaag taatggaatg
    13741 ataccaccaa catattgacc gattgcagtt gccagtgctg cacctaccaa tccccattta
    13801 aaaacagtga taaataatgc gtctaaaaca atatttgtaa ttccagccat aattgcaaca
    13861 tataatccaa gttttggctt ttctgctgtt gcaaataaac actgaaattc gcactgcaaa
    13921 atataggcag gaatagcgat aattaccaca catccataaa gaatactatc tgtcaataat
    13981 tgaccatctg ctcccatagc agaagcaatc catggcatag ttactatacc taaaacagct
    14041 agaataatac cacaaccagc agaagtaaca atgagtaacg aaaactgttc gtttgcttct
    14101 tctttttttc cttcacccat tgttttagca attaaagctc caccacctgt tccaaaaaga
    14161 aagccaacac accctaagat tagcaaaaat ggcataataa aattgacagc agtaaaagca
    14221 gttttcccta caaaatttga aacaaaatat ccatcaacaa ccccataaat agaacttatt
    14281 accagcataa taattgtagg aaatgtaaat tttaataagc gtttataatt aaaattatct
    14341 gacaactgta tcattgttca tacctcctaa accaatactc tgaaaagtta gcaattgcat
    14401 aagaaagatg ttttatattc cattcagatg tattaactat aatatgatag gaagtttgat
    14461 ttccccataa ggagctcgta aaaaattctc ttgatcttgc tcgattttta tcaatctctc
    14521 ttatctttct ttctaattct ttttttgaaa tatgttcatt gtttaacttt ttttcaatac
    14581 agcgttgtat tttaaagttc atatttgcac aaacaaaaac gttaaatggc tggtacttct
    14641 ttaataatat atctgcattt ctaccaacaa taacacaatc cttacctgta ctggcaatct
    14701 gattgataac atttgattgt tctattaaca aatctgtctt catggattgc atagacaaaa
    14761 tattagtaaa agaagtacac attgttaaag gaacgtttct ccaaccatga ttttctaata
    14821 tgtaggaaac atagttttcg tcaatttttt tctttctagc tatagcagtg ataatttctt
    14881 tatcgtagta gtcaaaattt aaaatatcag caagttgttt tccaagttct cgtccaccac
    14941 ttccaaactc tcggctaatt gtaataattt tcataatatc ttttcctcac atttcattga
    15001 atataaaagc tttctatgtt atcctaaagt atctggtaac cctaaagtca ataggagatt
    15061 taaaaatgat taaaaaatta tataagacaa gtgaatttgc agaattgtgt ggggtaaaaa
    15121 aacatacact ttaccattat gatgaaattg aattattaaa accgagtatt acccatgaaa
    15181 atggctatcg gtattataca attgaccaat ttgaaaaatt taatattatt tctgtgctaa
    15241 aaaaagcagg tacaccttta gaagaaataa aacaatatct caatgattta gataccaata
    15301 cctttcttaa tgtacttcac agaaagctaa tggatcttca agaagaagtg aagaagattg
    15361 aaaaaatgtg ttctatgttg agaaatgtca tttcaacaat ggaaatacat ctcaatgtaa
    15421 acacagaaaa aatcgaaatt gtaaattgtg aggaagaata tctgattgca actaaaattc
    15481 ctgaagctgt actaacagat gaaaaaacaa tgttacaagg tattggaaat cacttacaat
    15541 attgtttatt acatgattat aattttcatg taggagaaat tgttcttgaa aaaaatataa
    15601 aacagggaat ttttagagag agctattatt atagccttat taaaaataaa gtagatgatg
    15661 aacgcctttt tattaaacca aagggaaatt atgcagttat gtatcaccag gggagttatg
    15721 acagtttata taaaacttat caaaatatga aaaatcagtt agaagaacaa gggtatcata
    15781 ttgttggaaa tatttatgaa gaagatttaa ttgattattt atcagaacat aacgccgaaa
    15841 actacattca aaaaatttct atgcaggtta tttgaaaata taaaaagcgt gtatatctca
    15901 aatcacatga gaaatacacg ccttttttaa tatctcgtat tgtctataat aatttgctta
    15961 aaatgtcctt ttttatcttc taaagttggt atgttatttc caaatttttg tagaaattca
    16021 ggttcattgc tattatttgt ttcctcaatg caaactgcat ttcctcatga atttcagata
    16081 cagaaatagc tttttatttt cgttaaccat ttaatcagtt ttttactatc tttttataaa
    16141 atcttaacat ggaggcttta tgcctccatt tcttttgtag ttgtcttaaa agtagtgcaa
    16201 atttttttct taaatatata ttatataaat tattcattta tatagagtat tatattatgc
    16261 tctgtaaata gttacaatat ttaaaagaat gaaaggagaa caaaaactat ggaaattgat
    16321 tataaagcaa tcggacagcg aattaaaatt gcaagaatta aaaaaggtat aacacaagaa
    16381 tccgttgcaa acattatcaa catgacacca tcccatatga gcaacgtgga aacaggaaaa
    16441 acgaaagtca gcctgccaac cttgattgcg attgccaatg cactttctgt atcagtcgat
    16501 actctcttat gtgataatgt actttcttcc aaagtaattt ttgaaaagga agccaaagat
    16561 attttctcag attgtgatga atatgaaatc cgttctttag ttgaactgct aaaatcagca
    16621 aaaactgtaa tccgtaaaga cagagaaatc cgcaaacaat ttgaaaaata aaaccacagc
    16681 ttcaaggtat aaattgatat gtgttgttag ctaaggtctt ttcttttttt atataatcat
    16741 ttttatataa tcaaataacc tatccaataa ttttccaatt actgtcctta tgaaatatca
    16801 atttatacta tgatacctgt gtcgcttttg tctgattatc aaggaatttt acagctacct
    16861 tttcattatc aacgacaaca gtaaaaatag ggtttaccaa ttcagaaagt aaatattctt
    16921 gaaatatact attttccttt cataaaaatg ataaaatttt attttacatc ccacgtaatg
    16981 aagtgttcaa taaataagtt aaaataattt atttacctcc ttaatcatat tttttatgaa
    17041 tacctaataa cacagtatca atagaatcga ctagaccttt tatagcttct tctttactca
    17101 aaagtgattt cccaagttgc aaagtcgaat aaccatgaag tacactcatt attaagttta
    17161 atacttcacc tgtattcttt gtatttagat tacaagaaag aataagtttt tctaataagg
    17221 atttatattc atcaaagatt tctaatgttt cattatttcc atgccaagta gcccactgaa
    17281 ttgtttcata aacaccagga ttagaaatta taaaattaaa gtatgctata cttatacact
    17341 ttatagctgt atctccaaaa tttccaatag ccgcctttat catttgacta ttcatctctc
    17401 tcattccttt atgagctact tcaagtaata aatcatctag actttctata tgattgtaca
    17461 aagatggtgt acgtattcct agcttttctg caataacttt taaagaaact ttgttaagcc
    17521 catctttatt aactatatct gatgtagctt gtataactat ttctctacta acacgcatga
    17581 ttttccctct ttcatttaaa attaattact ttattagttt aactaatcta attagtttcg
    17641 tcaattattg taatttataa aattctttat actattataa aatcttgaca gtttaattat
    17701 aatttaatat aatactaatt gaattagttt taatactaat ataattagta ttatattaaa
    17761 ttctatatta caacattatt ttatagtttg attaatttaa caatcactaa tatatcaaac
    17821 tgaaaggaga tttttatgga aaaaaaatat ttcgaaattc atggagttcc ttcaatcctt
    17881 tggggaaatc cttcaagtaa attatatatt tacatacatg gacaatgtgg caataaagag
    17941 gaggctgaag cttttgctaa tattgcagtt ccacatgagt gtcaagtttt aagtgttgat
    18001 ttgcctgaac atggagaacg taaaagagaa aaaaattcat ttaacccatg gcatgttgtt
    18061 ccagaattag tatctattat ggaatatgca aagtgccact ggaatcaaat ttcgttatat
    18121 gcaaatagca ttggagcatg gtttagtatg ttaagctttg aaaaagagaa cttaagagag
    18181 tgtatttttg tatctccaat tctagatatg caacagctca tttcaaatat gatgctttgg
    18241 gcaaatgtat cagaagaaca gcttaaacat gaactaatta tcccaacttc atttgggcat
    18301 actctttcat gggaatatct acaatatgta aaacaacatc ctatcactca atggaatgta
    18361 ccaacaaaaa tattatatgg taaacatgat gaacttactg aaatcggtgt tgtagagaaa
    18421 tttgtagaaa agtttaattg caaccttact attatagaaa atggcaaaca ttggtttaac
    18481 acaccacaag aattaaattc attatatgaa tggttaaata ctttagttaa ataaaaaata
    18541 attatattat taatatgatt aaattattta taaatgttat ttaattaatt ttattttagc
    18601 attattaaaa taactacata aagaataaat tagttattat taaagaaatt aaaagtatat
    18661 agagtattaa taatttttta ataattagtt attatagaaa aaagagggat ataatattgt
    18721 ctccctctct ttcttctgta atattatata atttttattt atcactctca ataaattata
    18781 gttatctaca aaattcaatc tttaatacac tattaatata ttgtgatatt aatattacag
    18841 tattaaataa aatctaataa aattaactat aaaatatcta ttatataaat atccactctt
    18901 atgtttgaaa tccgtcatat agttttatcc ttcttatctt ttatatcaat aacttttttt
    18961 aataaaattg acaaaactaa agtaaaaaac cttattgata atgaaacaaa tgatactata
    19021 agttctgata ttatagtaat tgcatataaa tagacatcat aaaaataata taatattgtc
    19081 actataaaat taaatgtaaa aatctcaaat ctaactaaat acacatatag gtatttttta
    19141 agtttttagg tatttcttct gaaaatgatt tattgattga taccaattta cataatcttt
    19201 ttaatataaa caatgataca atatatggga cagcacatat gtatatagct acttatatta
    19261 catattacag ttggcatatt cgtatttgta atgcttatac caattttaaa atacaccatc
    19321 agtactaatg gtattaaaat aatctataat aaa
//
LOCUS       DAELNY010000064        19251 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.064, whole genome shotgun sequence.
ACCESSION   DAELNY010000064 DAELNY010000000
VERSION     DAELNY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.064"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..620
                     /locus_tag="KPE55_001896"
     CDS             99..620
                     /locus_tag="KPE55_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0492980.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            654..1430
                     /locus_tag="KPE55_001897"
     CDS             654..1430
                     /locus_tag="KPE55_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0492981.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1772..2725
                     /locus_tag="KPE55_001898"
     CDS             1772..2725
                     /locus_tag="KPE55_001898"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG0492982.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2743..3762
                     /locus_tag="KPE55_001899"
     CDS             2743..3762
                     /locus_tag="KPE55_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG0492983.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3793..4044
                     /locus_tag="KPE55_001900"
     CDS             3793..4044
                     /locus_tag="KPE55_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492984.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4326..6131
                     /gene="lepA"
                     /locus_tag="KPE55_001901"
     CDS             4326..6131
                     /gene="lepA"
                     /locus_tag="KPE55_001901"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG0492985.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6280..7617
                     /locus_tag="KPE55_001902"
     CDS             6280..7617
                     /locus_tag="KPE55_001902"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG0492986.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7850..9163
                     /locus_tag="KPE55_001903"
     CDS             7850..9163
                     /locus_tag="KPE55_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0492987.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAVGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9335..10510
                     /locus_tag="KPE55_001904"
     CDS             9335..10510
                     /locus_tag="KPE55_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG0492988.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10613..11632
                     /gene="hrcA"
                     /locus_tag="KPE55_001905"
     CDS             10613..11632
                     /gene="hrcA"
                     /locus_tag="KPE55_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG0492989.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11662..12282
                     /gene="grpE"
                     /locus_tag="KPE55_001906"
     CDS             11662..12282
                     /gene="grpE"
                     /locus_tag="KPE55_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG0492990.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12374..14221
                     /gene="dnaK"
                     /locus_tag="KPE55_001907"
     CDS             12374..14221
                     /gene="dnaK"
                     /locus_tag="KPE55_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG0492991.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14432..15586
                     /gene="dnaJ"
                     /locus_tag="KPE55_001908"
     CDS             14432..15586
                     /gene="dnaJ"
                     /locus_tag="KPE55_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG0492992.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16552..17487
                     /locus_tag="KPE55_001909"
     CDS             16552..17487
                     /locus_tag="KPE55_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0492993.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17523..18896
                     /locus_tag="KPE55_001910"
     CDS             17523..18896
                     /locus_tag="KPE55_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0492994.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLIFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19084..>19251)
                     /locus_tag="KPE55_001911"
     CDS             complement(19084..>19251)
                     /locus_tag="KPE55_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0492995.1"
                     /translation="DLNISKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYL
                     SVKSYFLYKKI"
BASE COUNT         7632 a         2125 c         3510 g         5984 t
ORIGIN      
        1 catttattat agttgtaatt actatttata tcatagactt gaatagtcta aaaaaataga
       61 ctataatatt tttatagact aaagaaaggg ctgataagat ggacatagca aaactaattt
      121 taaatcctgc aaggcttcgt attttacaat atattcgact ttatggcagt gttcgtacat
      181 ctgatattgt aaagtattta aatgatattc ctcgtgcaac agtttatcac catgtaaaaa
      241 ttttagaaga gaacaatatg attgaagtaa ttaaagaaaa tcgtgtgcgt ggtactattg
      301 aaaaggttta tgcactaaag gaatatacaa catctatgga aggagagacc ttcgttgcac
      361 tttctacagc attttatgta ggtctgatgc aggaaatgaa tgaatacttt agcagaaaag
      421 agcaagacca taaaaaggat aatgtatttt tctcttcagc attactatat atgagtgata
      481 atgaatatga aaatcttctt aaaagtatag ctgatttatt gaaaccatat atagaacaaa
      541 aacctaaatc aaatctaaaa cttagaaaac tttcaattat atcttctcca cctgtagctg
      601 ataatgataa aacaaaataa catgcaaccc atttttataa aaggaggtta tatatgaaat
      661 ttcatgagtt tggaaataaa aacagaccac atattatgtt gattcatggg ggagggaatg
      721 catggtggaa ctatttgcga caagcagaag ttttgtctaa caaatatcat gtgatacttc
      781 ctacattgga tggtcatgga gaagaatatg aaacaacata catttctaca gaggatacag
      841 cagataagtt gatatcatat atagatgaaa attgcagtgg acatcttttc gctttgtgtg
      901 gggtatcact tggtggtcaa attgtcatgg aacttttatc tcgaaaacct aatattacta
      961 aaaaagcaat tattgatggt agtatttgct acccaagacc tcttatggca aggttttgta
     1021 ttgctggtat tcgatttttt ggaggacttt tgtttagtga gaaggcttgt cgctttcaaa
     1081 ttgctatgat gccaaaattg ttacctgaaa atatgcaata tccagatgaa ataaaagcgt
     1141 attatatgac agatatgcca catgtaagaa aagaaacatt ctataatatg tatcgtactt
     1201 acatgatgaa ctatacactt aaagagaatg ttaaaaaaac cacagcacaa gtaatgtact
     1261 ggtatggaga aaaagaaatg aagtgtgtca aaaaatctgc aaaactgttt cagtcttatg
     1321 tgccctcttg tgagatttat gaagctaaga gctataatca tgggtattta tcactttatc
     1381 ttccaaatga atggttggaa attgcagaac catttttcca aaaggaataa aggtgttagg
     1441 tgattattgt ataattattg ctgttttttt atatagtaaa taaattaggt ataaatataa
     1501 aaaattattt tagttaatat attagtgaaa gcttaatagg aaaattataa atagattaaa
     1561 ggtagcacct atataaatag ttgctacctt tttctgtttg taaataaatg atagataaga
     1621 aggtttactg acaaatcact gactaaaccc aattttctta cattgaaagc cttaatttta
     1681 cttgttttaa ataaatttac aatttcttgt tataaaatac taaattttgt taacaattat
     1741 aattagcatt taatttatac tggaggttta tatgattagt gtaagaacag atttagcttt
     1801 agaagctagt gaaatgtgtg aaaagtctca agaaggaagt agtattcctg gtgtaaaaat
     1861 agaaactaaa gaattagaaa attgcattgt aactaaagtt gaagtaatag acaggcaagg
     1921 cgcagagata atgaataaag atataggaaa gtatataact ctggaaagta atcttatgaa
     1981 gtttgatgat gatgagtcaa gagaagaaat gataagttat ttaaaagagg agttagtaga
     2041 tatatttggc caagacaaaa ataaaaaaac cctggtaatt ggtcttggaa atagaaatat
     2101 tacttctgat gcgttaggac caaagagtgt atcaaaaaca cttgtaacta gacatctttt
     2161 taaaaattat aataaagact atgatgatga tttcactgag gtatcagctt taagcccagg
     2221 agttatgggt gttactggaa tagaaactag tgaaatagta aaatcattgg ttgagaaggt
     2281 taagccagat agagtagtag caatagatgc attggcttca agaaaaatgg agagagtaaa
     2341 ctctactata cagatatcaa cagcaggaat atcacctggt ggaggtgttg gcaatactag
     2401 aaaatctcta actaaagaaa ctttaggcgt ggatgttatt gcaataggcg ttccaacggt
     2461 ggttgatgca gcaactctta caatagatgt cttagatatg gcaatagaca atttaatagc
     2521 tcaatcagaa gaaacagaaa gtttttatga aatgttgaaa aaacttaaag aagaagaaaa
     2581 atatcatcta ataaaagatt cgttagaccc atatgacaag aacttaatag taacaccaaa
     2641 ggatatagat gatacaatcg aaaatctatc tataataata agtgaaggct taaataggtc
     2701 tttacatcca ggtagattgg tataaaataa ggaggagcaa ttatgtttaa aaaatgcatt
     2761 aaagtagtaa ccttgacttt tatattagta tgcattttac caggaaaatc attagctttg
     2821 aatcaagatg attttttaaa gtttttagta aattcatctt atccagaagc taaagtcgaa
     2881 ggaaatgata cagaaaataa aaagaataat aaaaataaag aaacaagtaa ggaaaataag
     2941 gaagaaagta aagaaggaaa cactaaatct aaagatgcaa gtaaagttga taataaaaaa
     3001 gagagtgaaa aagaatatat aaaactttat gttggtaaag aaaatgttcc agatattgag
     3061 tcaaaaaatt cagacacaac tgaaactaac acaacatcta gttcagatta taaggatgat
     3121 ttaagagtaa ccaaagaaaa tccaagaatt ttgatatatc atactcatgg atgtgagact
     3181 tattcaaatt cacctgatgg aaattatcat tcaagagata agaaaaattc agtaatggaa
     3241 gtaggaagtg cattaactag tgctttagat agcaaaggat ggggtgtagt ccatactact
     3301 aaataccatg actatccatc ttataataac tcttatgcaa gtagcttaaa aacaatacaa
     3361 agtatattac ctaaatataa ctcagtggat atagcgatag acttacatag agatgctagg
     3421 gatttaacta atccagccac taaggaaaaa gaccatttaa aatatacaac tatgataaat
     3481 ggagagagag tatcaaagtt cttctttgtt gtaggaggta aaaatacaaa tagaaaacaa
     3541 cttagagctt tagcagaaga tataactgct tttgctgaga aaaagtatcc tggtctagta
     3601 tctccaatag tagaaaaaga ctatgctaga ttcaatcagt ttgcagttaa gaatcatatg
     3661 ttagtagaaa tagggaataa cgcaacaagt gtagaagaat ctaaggctac tacaaagtat
     3721 ttagccgaga ttttggatga atattttaaa caaaaaaatt agagctttaa gttataatta
     3781 ggagcatgct aaatgagccg attagaaaaa aatatagaaa gtaaaagtaa actaagaaaa
     3841 tacaagtata ttatgagaat cttatttttg attactatga ttatatgtac atcagtttgt
     3901 gtatttatag tggacagtaa tgcaaaaagt atgctaggaa tgaaacaata tgattttcca
     3961 agtattaaat ttgatataac aaaatttgat tttgacatac caaattttga tatacatgaa
     4021 atcataaaaa aatttaataa gtagtaataa gtgaataagg tttattgatt aaaataagcc
     4081 ttattttagt tatatatttt taatgtataa aataaaaatt cattaactgt attttatagg
     4141 atttacttta agtttatgat attatataat atttagtgtt agacttgata atccacatat
     4201 aatgtggata attttgttta gtgccaatta gtttatattc aaaaatttta aatttggtat
     4261 ataaagagtt tttagataat gtaaaataat aactataaga ataatcaaga ggagggaatt
     4321 ttaaggtgga caataaacaa agtagaacta gaaattttag tataattgca catatagacc
     4381 atggaaagtc taccttagct gatagattaa tacaacaaac tgggcttgtt agtgaaagag
     4441 acatgaaaag tcaattactt gacaatatgg accttgaaag agaaagagga atcactataa
     4501 agcttcaaaa cataagatta atgtataaag cgaaggatgg aaatgaatat tatttaaatc
     4561 ttatagatac acctggacat gtagacttta actatgaggt atcaagaagt ttagctgcat
     4621 gtgaaggagc actattagta gttgatgcag ctcaaggtgt tgaggcacaa actttagcca
     4681 atgtttatct tgctatagac caagatttag aaatattacc aataataaat aaaatagatt
     4741 taccaagtgc aagaccagag gaagtaaaaa atgaaataga agatttaata gggcttgatt
     4801 caagtgaagc tcctcttatt tcagctaaaa ctggtttaaa catagaagat gttttagaag
     4861 atattgtaaa aaatgttcct ccacctaaag gagataatga agctccatta aaggcattaa
     4921 tatttgattc ttactatgat gcttacaaag gtgttgtagc ttatgttaga gtatttgaag
     4981 gaactgttaa aaagggtatg acaataaaaa tgatgaatac caataaaaag tttgaggtaa
     5041 ctgaagttgg tgttatggct ccaggtcaaa ctgaacttag tgaattatca gcaggtgatg
     5101 ttggttatat tgctgctagt ataaaagata taagaagttg tcgtgttggt gatacaataa
     5161 cagattcaaa taatcctaca gaagaacctt taccaggata caaaaaagct actccaatgg
     5221 tatattgtgg tatatatcca ggagaaggag aaaagtatga gaatgttaga gatgctcttg
     5281 aaaagcttca agtaaatgat gctgcacttg aatatgaagc tgagacttca gcagcactag
     5341 gatttggttt tagatgtggt ttcttaggtc ttttacatat ggagataatg caagaaagac
     5401 ttgagagaga atttaacctt gatataatta ctacagctcc atctgttata tatagagtta
     5461 caaaaatgga tggagaggtt gtaatgatac aaaatcctgc aaacttacca gaaccatctg
     5521 agataaagat gatagaagag cctatagtaa aaggagatat aatagttcct aaagactatg
     5581 tgggtgttgt tatggagctt tgccaggaaa gacgtggaaa tatgttaaat atggaatata
     5641 tagatgatag aagagtaatg cttcactatg accttcctct caatgaagtt gtttacgatt
     5701 tctttgatgc tttaaaatca agaactagag gttatggttc tcttgattat gaggttaaag
     5761 gttatgtagc atctacactt gtaaaacttg atatattaat caataaagag caagtggatg
     5821 cacttagttt tattgtccat gagacacgtg ctttccctag aggaaaggca atgtgtgaga
     5881 aattaaaagg agaaattcca agacatcagt ttgctatacc aatacaagct gctgttggta
     5941 ataaggttat tgcaagagaa acaataagcg ctctaagaaa agatgttctt gctaaatgtt
     6001 atggtggaga tatatctcgt aagaagaaac ttcttgaaaa acaaaaagaa ggtaagaagc
     6061 gtatgagaca aattggttct gttgaagttc ctcaaaaagc atttatgtct gtgcttaaat
     6121 tagatgagta aatccttata aatttagata aagatattta aaagctagag tgattttaga
     6181 attatttcta aattagtcta gcttttttat ggtatatttt aagtaaactc atagagaata
     6241 taagtaagaa tattaaagaa gaataaaaag gtgatattta tgattaatat agaaattcct
     6301 aaaaaagtag actatataat taaagaatta gaaaagaatg gttatgaagc ttatatagtt
     6361 ggaggatgtg ttagagattg tttattagaa agagttccaa acgattggga tataacaact
     6421 agtgctagac cagaagtggt agtagaatta tttgaaaaaa caattccaac tggtatacaa
     6481 catgggactg taacagttat gattgaacat gaaccatttg aagtgacgac ttatagaatt
     6541 gatggaaatt atagtgatgg tagacatcca gattcaatag aatttacaaa taacatagtt
     6601 aaggacttgt caagaagaga ttttactatc aattcaattg catacaattc aaagacagga
     6661 ttagtcgacc catttaatgg ctatgaagat atacaaaata agtatatacg ttgtgttgga
     6721 aatccagtag atagatttga agaagatgca cttagaatgc ttagagcagt tagattttca
     6781 gcacaactca attttaaaat agctgaaggt actaagcaaa gtattcataa aaaagcagat
     6841 ttgataaaaa atgtttctat tgaaagaatc cagacagagt ttaataaaat attagtatct
     6901 gattcatcta aattaaactt attaaaatca acaggtttgt tgaaatttat aattcctgaa
     6961 atatgtgaat tagaagatgt tactcaacac aatccatatc acatctatga tgtacaaaaa
     7021 catactttaa tagctacaga agcaattgaa gatgaactgt atttaaaact tacaatgttg
     7081 tttcatgatt taggtaaaaa agtaactaaa acaactgata aaaatggtgt tgaccatttt
     7141 tatactcata gtagagaatc tgtaaagatt gcaaagaaga ttttgaaaag acttaagtat
     7201 gataattata ctattaataa agttttaata ttaatacaat atcatgatta tagaatagaa
     7261 cctaaaagga agataattaa aaagttatta aataaacttg aagatgttga attatttgaa
     7321 gatttaataa aagttaattg ggctgataca cttgccaaaa atccaaagta tgcaaaacaa
     7381 aagatattaa atcttattga gtgtgaaaaa gaatttaaac atataataaa tcaaaaggag
     7441 tgctttaatc ttaaagattt ggctattaat ggaaaagatt tgatatctat aggtataaaa
     7501 ccaggtaaag atataggaca tattttaaat aagatgcttg aaatagtcat aaataatcca
     7561 gagttaaatg aaaaagaaat actaaaagaa aaagcattaa atatatatac attttagatt
     7621 caaatataaa aaatttaatt actttaaaaa acatagaata aaatacttat ttagataaaa
     7681 taaataattt ttagagaata ttttagaagt tacgattgta tgatttttaa atgcataggc
     7741 atttactcta ggactataga ctaatataaa aaaaaacttt gggaatacta aaaccataaa
     7801 tttataaaat taatatttta tatactgtaa aacagtgagg agggacagta tgcatactca
     7861 actacaaaag actattggtc tttcagcagc tctttctact gttgtaggta tggttatagg
     7921 ttcgggagtt ttttttaagc cacaagcaat atacactaca actaatggag ctccaggtct
     7981 aggtattata gcgtggcttt taggtggttt tataacaata actgctgggc ttacagctac
     8041 agaaatttca gctgctatac caaaaactgg tggtatgatg atttatatcg aagaaatata
     8101 tggagaaaag ttaggttttt taactggatg gatgcaaact gtattatttt tcccaggtac
     8161 atcagcagca ttaggagtaa tatttgctca acaagcatct gaacttttag gaatgagccc
     8221 aaataatatg gcaaatgtac ttcctatagc agttggagtt atactatttt tagcattatt
     8281 aaatataata ggttcatcct taggaggaaa agttcagaca gtagccacta ttggaaagat
     8341 gataccttta attttaataa tagtctttgg atttattaaa ggtcaaagta gtgaagtatt
     8401 aaatccattt gtaggagatg gtgtaaatgc atcaaatgca cttggacaag tattaatagc
     8461 tactttgttt gcatatgatg gttggataaa tgttggagcc atatcaggag aaatgaagtc
     8521 tccagagaaa gatttaccaa gagcaatagt tggtggctta tcattagtta tggcagttta
     8581 tattataata aatatagctt atctttgggt agtaccagca agtgaattag caacagttac
     8641 ttccccagct acattagtag caacaagatt gtttggaaat ataggtggaa aagtaataac
     8701 tgtgggtata ttaatatcag tatttgggac attgaatgga tatttattaa caggttcaag
     8761 gataccttat acattagctg aaatgggaac tttaccagca tctaaaactc ttttaaaagt
     8821 aaattctgga ggttctcctg taaattcaat actacttata actgtgttag cctgtgtata
     8881 tgcattatct ggtcaattta atcttttgac agacttaaca atattttcaa tatgggtatt
     8941 ttatgtattg acttttatag gtgttatgag gcttagaaga caaaagccag atttacacag
     9001 accatataaa gtccctttat atccaataat accaattata gctatattag gtggactgtt
     9061 tgtaataata aatcaaatat tgacttcaac agtcatatct ttaggtggga tatttataac
     9121 actattaggt ttaccagttt attattatat gaaaaaaaga taactactta atttaagtta
     9181 gataaatttg ggtaaaggtg aaaattaact tttatccaaa ttttttatac tgttatacaa
     9241 tataagtatt atatattact tgatgtgcta ttttgtttga tttataattt agtaattgat
     9301 gttttaagat aatttaagaa atgaggagtg aaagatgcta ggactttatg tacatattcc
     9361 attttgtgtg aaaaaatgta agtattgtga ttttaattct tataaaatgg acatagattc
     9421 aaaaaaaaga tatatagaag acttaaaaat agaaatggaa ttatatagta ataaattata
     9481 taaagataat aaatataaaa ataaagaatg ctgtagtcta aataaaaatg acaaaataac
     9541 gagtatattt gtagggggag gtacacctag tatactaacc tctgatgaaa taagagaagt
     9601 atttataagt ataaaagaaa tgtttgatat agatgagaat gcagaaataa ctatagagtg
     9661 taatcctggg acattaacat tagaaaaatt aaaaactatg aaagagattg gcataaatag
     9721 acttagtata gggctacaag ctattcaaga aaagcatttg aactttatag gaagaataca
     9781 tacttatgaa gaatttgaga aaaactataa agatgcatta agtgtaggtt ttaaaaatat
     9841 taatatagat ttaatgtaca gtcttccaaa tcaaactcta tgtgattgga aggaaactct
     9901 tgaaaaagtt gtacacttaa atccaacaca tatatcagct tattccctta tactagaaga
     9961 aggaactgaa ctatacaata tgtatgaaag taataaattt gaacttatag atgaaaatgt
    10021 agatatagaa atgtatgagt atacaataaa ctacttaaag tcaaaaggat ataatcaata
    10081 tgaaatatca aactactcaa aagaaggcta taattgtgag cataatattt tatattggga
    10141 atgtgaacat tatataggga ttggagcagg agcttctggc tatattaatg agaacagata
    10201 caataatgta gaatctttag aagattatca tttaagtttg gtaaaaagag aaaaaccaat
    10261 acaagaaaat gaaattcttt ctgagaaaga tatgatagaa gaaaagatat ttatggggct
    10321 tagaatgaat aaaggcataa aatttgaaga ttttaaaaaa aaatttggaa tagattttag
    10381 agagaagtat aataagcaaa tagaaatgct attagctaga aagcttataa atcagagctt
    10441 tgaaggtata caattaactc aaaaaggaag agaaatatct aatagtgtat ttatagaatt
    10501 tatggaataa tttttaaggt gttgacaaaa taaatttata atggtatata atatatataa
    10561 tctttttagc actcgaactt agtgagtgct aacagctagg aggtataata atatggaatt
    10621 aaatgaaaga aaattaaata tcctcaaagc tatagttaaa gattatatag aaacagctga
    10681 agctataggt tctagaacaa tatctaaaag acatgattta ggtgttagtg ctgctaccat
    10741 aagaaatgaa atggctgacc ttgaagaatt agggtattta attcagcctc atacttctgc
    10801 gggtagagtt ccatctgaaa aaggctataa gttatatgta aactctctta tgagtaaaag
    10861 cgaattggat gataatgata agattcttat tgaacaatgt atgaatcaca atataaatca
    10921 tataaaagaa ttgattcatg aaacttctaa gctattatcg caactaacaa actatacaac
    10981 tgtagcagtt actaagagtt taataaatca aagtgttata aagcacatac agttggttgc
    11041 tatgaatgac aataatattt tacttatagt tgtaacagat aaaggggact taaaaaaagc
    11101 taaccttaca actaatgtat atttagatca atctaagtta aatttgattt ctgataatct
    11161 tacaaggaag ctcttaggta aaagtataac agatttggat gataatctaa ttgcttttat
    11221 taaatacgaa ataagtgagt attctgggct tatagatgag cttttaaatg ctttgaactc
    11281 aaatatgaaa gaagaagatt tttctctatc attaaatgga gctactaaca tatttagtta
    11341 tccagaattt aatgatgtat taaaagcaaa gtcattttta aatatgttgg aaaaaaagga
    11401 aacaatagct gacataatta aatcaaaggg aatacaaaaa gacaatctaa atataataat
    11461 tggtagtgat aatgattgtg aactggcaca ggattgtagt atagttacag caacttataa
    11521 tgtagataga gacttggttg ggcgaataag ctttataggc cctaccagaa tggattatgc
    11581 tagaatttac tctataataa attatatgag tttattaatt aatagaaagt aaagaaattt
    11641 taaattgcga ggtgtaaagt cttggataaa aaaaatgagc aacaggaagt aagagaagaa
    11701 aatgatactt ctattaacca agaatcggaa actcaagtag agttagaaga ggaagttgtt
    11761 aatgaagaat gtgaaacatc aagtgaaaaa actgatgaaa aagaagttga tgatgaaaat
    11821 gtaacagata taaattctaa gttagcagaa aaaaaattgc aggatgagtt ggatgaatta
    11881 aatgataaat atcaaagact tcaagccgaa tatgcaaact acagaagaag aactcaacaa
    11941 gagaaagaga caataggcgt atttgcaaat gaaaaaataa taactgaatt gataccagtt
    12001 atagatagta tggaaagagc cttagatgca tgtgaagata aggaagatac tatgtataag
    12061 ggtataagcc ttgtacataa acaattaata gacactttag taaaatttgg agtagaagaa
    12121 atagaagcag aatctaagga atttgaccct aatctacatc tagcagttat gcaagaaagt
    12181 gtagatggag ttgaagcaaa tcaaatagta atggtacttc aaaaaggata taagctagga
    12241 actaaagttg taagaccttc aatggttaaa gtttcttgct aagtataaat taaaggttaa
    12301 attatttaac ctaatataaa atattaaaat aagtaaaact taattatata attactcgag
    12361 gaggatttta gtcatgggaa aaataatagg aatagattta ggaacaacaa attcatgtgt
    12421 tgcagtgtta gaaggtggag aagcacaaat aatagcaaat agtgaaggta tgagaactac
    12481 tccatcagta gtagcattta caaaagatgg tgaaagaata gttggagaac ctgcaaaaag
    12541 acaagcagtt acaaatgcag ataaaacaat aacttctata aaaactcata tgggaactga
    12601 ttataaagta aatatagatg gaaaatcata tactccacaa gaaatatcag caataatatt
    12661 acaaaaatta aaatcagatg cagaaagtta cttaggacaa acagtaacag aagcagttat
    12721 aacagtacca gcttacttta cagatgctca aagacaagca actaaagatg ctggtagaat
    12781 agcagggctt gatgttaaga gaataataaa tgagccaaca gcagcagctc ttgcttatgg
    12841 tatggataaa ttagaccaag aaaagaaaat attagtattt gacttaggtg gaggaacttt
    12901 tgacgtatct atacttgaaa taggagatgg aacttttgaa gttttagcta cagctggtaa
    12961 caatagatta ggtggagatg actttgacca aatagtaata gattatttag cagaggaatt
    13021 taaaaaagct gaaggtgtag atttaagaaa tgataaaatg gctcttcaaa gattaaaaga
    13081 agcagcagaa aaggctaaga aagaattatc atcaacaatg agttcaaaca taaacttacc
    13141 tttcataact gctacagcag aaggaccaaa acacttaaat atagatttat caagagctaa
    13201 atttgaagaa ttaactagag gtttagttga aaaaactatg gaaccaacta agacagcttt
    13261 acaagatgca ggactttcta caggtgatat agatgatgta ttattagtag gtggttctac
    13321 aagaatacca gcagtacaag aagctgttaa gaagtttata ggaaaagaac ctcataaagg
    13381 tataaaccca gatgagtgtg ttgcagcagg tgcttctata caagctggag ttttagcagg
    13441 ggatgttaaa gacttattat tactagatgt tactccatta tctcttggta tagaaactat
    13501 gggtaatgta atgactaaga taatagaaag aaatactaca ataccaacta agaagtctca
    13561 aatattctca actgctgctg ataaccaaac agctgttgat atacatgttc ttcaaggtga
    13621 aagaagtatg gcttatgaca atacaacttt aggaagattc caattaacag atataccacc
    13681 agcacaaaga ggaatacctc aaatagaagt tacttttgat atagatgcaa atggtatagt
    13741 taatgtttca gctaaagatt taggtacagg aaaagagcaa aaaataacta taacatcaaa
    13801 cactaattta tcagaagcag aaatagagca aaagataaaa gaagcagaaa tgaatgcaga
    13861 agctgacaaa caaaagaaag aaaaaataga agcattcaat caagctgaat ctactatata
    13921 tcaaactgaa aaaactttaa atgaacttgg agataaaata agttcaggtg aaaaagagga
    13981 tatagaaaaa gctatagcag atttaaaagc tgtaaaagat aatcaagatg ctacagcaga
    14041 agaacttaag aaagctacag atgaagtaat gactaagttc caaaaagtat ctcaagaaat
    14101 gtaccaaaaa gcagctcaag aacaacaagc ggcacaaggt gcagaacaag cacaagacaa
    14161 tggacctaaa gatgacaatg tagtagatgc tgactttaaa gaagtagatg aagataaata
    14221 agctatataa tatgtaacac taaatgataa ttggaacata tatgatttgt gaaaatcata
    14281 tagatgttaa agatatatgg acaatataca tcataaagaa tataatatat catagattat
    14341 ataacttaaa gtagaatata agaattaatg atagcttgta gtttattatt ctacaagcta
    14401 tcgttttgta aaatgaaggt ggtgtcaatt tttgagtaca aaaagggatt attatgaagt
    14461 cttaggtatc agtaaaggtg cagaagctca agagataaaa aaagcttata gaaaattagc
    14521 aatgaagtac catccagata gaaatcctgg agataaagag gcggaagaaa aatttaaaga
    14581 gataaatgaa gcttatgaag ttctatctga tgatacaaag agaaaaactt atgaccagtt
    14641 tggacatgat gggctaaatg gtcaaggcgg ttttggtggt caaggaggat ttggcggtca
    14701 aggctttggt ggttttgaag atatgttcgg agatatattt ggtgacatgt ttggaggaag
    14761 cttcggaggt ggaagagcaa gaagaagagg acctcaacgt ggtgcagata taagacagag
    14821 tgttactata tcttttgaag aagctgcatt tggtaaaaag atgtctataa aagtaaatag
    14881 aagtgaagag tgtgaagaat gtaatggaac tggagcaaaa ccaggaacat caaagaaaac
    14941 ttgttcaact tgtaatggaa ctggtcaagt tagaactgtt caaagaactc catttggtaa
    15001 tatagcaagt tcaagaccat gtagtgcttg taatggaact ggagaagtga ttgaatctcc
    15061 atgtagtaaa tgtcatggaa ctggaaatac aagaaaagtt aagactatag aagtagatat
    15121 acctgctggt atagatgatg gtcaaatgat aaaactttct ggtcaaggtg aagtaggaga
    15181 aaaaggtgct ccaagaggag acctatatat tgtagttaat gttaagtcac atccactatt
    15241 tactagagat ggaaatgata tttattttga aatgccaatt acatttgttc aagcaacttt
    15301 aggtgatgag atagaagtac ctactttgga tggaaaagtt aaatatagtg tgccagaggg
    15361 gacacaaact ggaactgtat ttagacttaa agagaaagga atccctagaa ttagaggtaa
    15421 ttcaagagga gaccaatatg ttaaggttgt agttgaaatt cctaaaaaat taaacgataa
    15481 gcaaaaagaa ctattgagag aatttgcgaa ggagtgtggg tcgaacgtcc acgaaaagaa
    15541 gaaaaccttc ggacaaaaga tagaagatat gtttaagaaa aagtagttaa agtagccaat
    15601 atataatcat atttttaaaa tcttagttat ttataagatt gtcaaaggag atattatgat
    15661 ttttatattg gctatttttg tataaaaaat aaatgtgcat aaaataaaac tgaatataaa
    15721 taattttctg aaaaatactt gtcctaaaat atataatgtg ctataatata atcaaataaa
    15781 agaaaggggt gattccagtg ggaacggatg tggcactgcc aaatgatgaa gaaaacttta
    15841 ttgtaatata caaaataaaa ttagatactt ttagtgctag attaagtact ggaaaaactc
    15901 atgagctggg ataattattg taattaccta gactcaaact tttagtagta aaatagtaat
    15961 attatataga caaggtatct aatttttaaa tcttttgtat gaattaaaat ttttataaag
    16021 ggtatatctg attagaatgt aattagggaa acctagttgt gaattacttt tagtatatac
    16081 atgtgtaaag aaatatgtat atggtattgg ttattgtgct agatgtgtat gtaaagaaat
    16141 atgtgtctag ttgggtatat gtaaagaaat atacttataa tctatgaaaa atttttagta
    16201 taaattgcta aaacattgag attgtttttt atagaaagtt agtttatacc aactaaaaat
    16261 gtgcttaaga tttgtttgat gtttaattat aaataaggtt gtagataaaa aaataagtaa
    16321 agacaaaggt ttaaaatact tgctttaaat atagtttgtt ttgaacactt gccaaattaa
    16381 aataattaat aaaatagaca tcttctcata gtaaaatata ctgtgagagg atgtttttgc
    16441 tttgttcata aacttttatg aaaaatttgc atagtgtcac attttaatat actatatata
    16501 taataaaata tagataaaat taaatgattc aaaagagaaa ggtgaaaaga gatgtattat
    16561 ataggtgtag acataggagg aacaggtata caagcaggag tagttgataa ctatgggaaa
    16621 ataattttta ggtcagagtg taaaacagta attgaaaaag gatttgaagg aatacttaat
    16681 gacataaaaa taatgattta taagttgttg gaagataata aacttactat gagtgacata
    16741 aaatctattg gatttggtgt tccaggtttt ataaataaag aaggattagt aacttgtgta
    16801 aatttaaaat ggaataaaaa agcttttaat aaagagttaa aaagaagatt tccagatgtt
    16861 gaaattcatg gagaaaatga tgcaacagta gcagcacttg gagaggcaaa gtttggaagt
    16921 atgaaaggtg ctaatgttgg agtattatat actttaggaa ctggtgtagg tggaggaata
    16981 gtaattaatc aaaaagtctt ttctggagca catggattgg gttcagaaat aggtcatcaa
    17041 attataggag aaaactattt taattgtaat tgtggaaata atggttgtgt agaaactttt
    17101 tgttcagcaa ctgcaattat aaagtattct caaaaactga tagaagaagg agaaaagagt
    17161 aggattttag atttggcaga gggaaattta gaaaatgtaa atgcaaagat ggtgtttgat
    17221 gcatatagag aaaatgattt agtggcaatt aaagtaataa atagatttaa agagtattta
    17281 gcaaaaactt ttgcaaacac aataaattct ttagacccag aaattatatc tataggagga
    17341 ggcatttcta aatctagcga tataatactt gatggaatag aagatttggt aagaaaattt
    17401 gtattataca aaacagaaga tatagctaca attacatgtg caactttagg ttcagacgca
    17461 ggaattatag gagcagcttt tttataatgt aaatgaactg cttattggag gcgtgatgat
    17521 atatgggtaa aacaaatgct agaagaacgc tttttttgat agcaataggt tcaggaacta
    17581 tgctgaatcc tcttaattct tctatgattt cacttgcttt acatagtata caaaatgatt
    17641 ttcatattag ttttacaact gtatcttgga ttgtttcagc attttaccta gctagtgcta
    17701 ttgcacaacc agtttcaggt aagataggag atttaatagg tagaaaggtc ttatttttga
    17761 gtggtttgat gcttgtaatt ttatcagcat ccatagtacc acttgttcaa tcattcttta
    17821 tattgatttt tataagagta attcaagcaa ttggaacaag taccctatat ccatctggag
    17881 ttgctcttat acaaaataat ataaaagaaa gacaatcttc tgcattagca gttctaacca
    17941 tttttgcttc aactacagca ggattaggtc cgacacttgg aggattattg ttggacttag
    18001 gaggatggca tgctatattt cttgtaaata tacctgttgt tttggtttct atatgcttag
    18061 ggtatttttt attcccaaaa gaagtaaaag aaaagaaatc tataaaagaa actttaaaaa
    18121 atgttttgtc aagattagat attattggca tacttctatt ttcaattgca atgattttta
    18181 tattactatt tttgctatct atgaaggagt cttttaactt agaacaactt atatttggta
    18241 taatactgat gtgtcttttt atttggcatg aattgagaac aaaattgcca tttatagata
    18301 ttaagctttt tgtaagtaat cctaaattgt caaaagttta cttgcaattt attatattaa
    18361 atttctttaa ttatattttg ttttttggtc ttcctagtta ttttcaagat gccttacatt
    18421 atagtgcaaa gtcgactgga ttatttatgc tatttatgtc tggtataggt atatttatat
    18481 ctccattaac tggaaaatgg atagataaat ctggaactag atttcctctt gttacatcat
    18541 caatctttat gtttattagt gcagtacttc tttcagtagt ttttatccat atacctgtaa
    18601 taggaaaggg tattatatta tcattagcag gtattagtta tggagttggt aatgtagccc
    18661 tgcaatcctc tatgtttgaa gaaagtcctc gtgacagtat agggacagct tctggtttgt
    18721 ttcaaactag tcgttattta ggttctattt tagcaactgt tttacttgga atggtatttg
    18781 aagtaaatat aacatcagaa caatttcaga tattaggtta tgttatggtg gtcttaggta
    18841 caataagttt tttattaaat tttatattaa agaaagaatt aagaaaagta gaatagtgga
    18901 tttatggatt taataagtct aataactgat aagtttaaaa atattgtttg taaaacataa
    18961 ctggtttggt atttattaat tctaagtttt gaagcataga gtactcgata agtatacaat
    19021 attttgaatt aaaattaagc agaaatttga actagtcttt caaatctctg cttaatttag
    19081 tagttaaatt tttttataga gaaaataaga tttgacactt agataaaaaa tcattgcatt
    19141 ttcaaaattc tttaaatcaa acccaagtat ctcattaatc ttttccaatc tatatactag
    19201 cgtgtttcta tgtaaaaaca tactagtaga tgtcttagaa atatttaaat c
//
LOCUS       DAELNY010000065        18854 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.065, whole genome shotgun sequence.
ACCESSION   DAELNY010000065 DAELNY010000000
VERSION     DAELNY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.065"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPE55_001912"
     tRNA            27..101
                     /locus_tag="KPE55_001912"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPE55_001913"
     tRNA            116..200
                     /locus_tag="KPE55_001913"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPE55_001914"
     tRNA            210..293
                     /locus_tag="KPE55_001914"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPE55_001915"
     tRNA            317..391
                     /locus_tag="KPE55_001915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPE55_001916"
     tRNA            416..492
                     /locus_tag="KPE55_001916"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KPE55_001917"
     tRNA            502..577
                     /locus_tag="KPE55_001917"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KPE55_001918"
     tRNA            586..661
                     /locus_tag="KPE55_001918"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KPE55_001919"
     tRNA            664..752
                     /locus_tag="KPE55_001919"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KPE55_001920"
     tRNA            756..831
                     /locus_tag="KPE55_001920"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KPE55_001921"
     tRNA            838..911
                     /locus_tag="KPE55_001921"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KPE55_001922"
     tRNA            926..1002
                     /locus_tag="KPE55_001922"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KPE55_001923"
     tRNA            1020..1096
                     /locus_tag="KPE55_001923"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KPE55_001924"
     tRNA            1105..1180
                     /locus_tag="KPE55_001924"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KPE55_001925"
     tRNA            1188..1261
                     /locus_tag="KPE55_001925"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KPE55_001926"
     tRNA            1269..1345
                     /locus_tag="KPE55_001926"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KPE55_001927"
     tRNA            1358..1433
                     /locus_tag="KPE55_001927"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KPE55_001928"
     CDS             1509..1922
                     /locus_tag="KPE55_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0492996.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2230..5661
                     /locus_tag="KPE55_001929"
     CDS             2230..5661
                     /locus_tag="KPE55_001929"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG0492997.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5903..7831
                     /locus_tag="KPE55_001930"
     CDS             5903..7831
                     /locus_tag="KPE55_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0492998.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8284..8748
                     /locus_tag="KPE55_001931"
     CDS             8284..8748
                     /locus_tag="KPE55_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG0492999.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8755..9249
                     /locus_tag="KPE55_001932"
     CDS             8755..9249
                     /locus_tag="KPE55_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG0493000.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9265..10290
                     /locus_tag="KPE55_001933"
     CDS             9265..10290
                     /locus_tag="KPE55_001933"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG0493001.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10277..12724
                     /locus_tag="KPE55_001934"
     CDS             10277..12724
                     /locus_tag="KPE55_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG0493002.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12904..14277
                     /gene="radA"
                     /locus_tag="KPE55_001935"
     CDS             12904..14277
                     /gene="radA"
                     /locus_tag="KPE55_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG0493003.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14280..15350
                     /gene="disA"
                     /locus_tag="KPE55_001936"
     CDS             14280..15350
                     /gene="disA"
                     /locus_tag="KPE55_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG0493004.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15439..16533
                     /locus_tag="KPE55_001937"
     CDS             15439..16533
                     /locus_tag="KPE55_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG0493005.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16662..18839
                     /locus_tag="KPE55_001938"
     CDS             16662..18839
                     /locus_tag="KPE55_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493006.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDSIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         7670 a         2115 c         3610 g         5459 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa acagtataaa atcaagtcaa ttgtagaaat tttcacataa
     2041 attcatctga aattctataa aaaatgcctt aattttaatg aggtaaatcg agtttacaca
     2101 gtctaaaaat tacttatatt tataaattta tcttattaat taaggaactt tcttaaacat
     2161 cttcaaaaat aaagtatttt agtctataat ctttaatatt acacaaacaa taaaggagaa
     2221 gaatagagta tgcttaaaaa gtttaataaa atactagtgg ctaatagagg cgaaatagct
     2281 ataagaatat ttagagcctg ttcagaattg gggataaaaa gtgttggtat atacagtaag
     2341 gaagataagt atggattgtt tagaacaaaa gcggatgaat catatttgat aggagaaggt
     2401 aaagggccaa tagatgcata tttagatatg gatggaataa tagacctagc aaagagaaaa
     2461 aaagttgatg ctatacatcc aggatatggt tttctagcgg agaatgcaga atttgctcgt
     2521 aaatgtgaag agaatggaat tacttttata ggaccatcat ctaaggtaat gaatatgatg
     2581 ggggataaga ttaactctaa aaaaatcgca aaagaagtta atgttcaaac tataccagga
     2641 gtagaaaaag caataagaag tactgaagaa gcaaaggaag ttgcaaacaa aataggatat
     2701 cctgttatga taaaagcatc taatggcggt ggcggtagag gtatgagaat tgtacatcgc
     2761 gaagaagact tagagcttga atatgaaact gcttgtagtg aatcaagaaa agcttttgga
     2821 gaagatatta tatttataga aaaatatata gctgatccaa aacatataga agttcaaata
     2881 ttaggtgata attatggaaa tatagttcac ttatatgaaa gagattgttc agttcaaaga
     2941 agacatcaaa aaataataga atatgcacca gctttttcgt tagatgataa agtaagaaaa
     3001 gagatatgtg aagatgcagt taagttatca aaacatgtgg gttattctaa tgcgggaaca
     3061 ttagaatttt tagtagatgc aaatggtggg cattatttta tagagatgaa cacaagagta
     3121 caggttgaac atactgtaac agagatggtt acaggaatag atatagttca aagtcaaata
     3181 ctaatagccc aaggttacag cttagatagt gaagaaatta atataaaatc acaagatgat
     3241 gtggaaataa gaggttactc tatccaatgt agaataacta cagaagaccc taagaataaa
     3301 ttcatgcctg atacaggtaa aattcaagtt tatagaactg gttcaggatt tggtataaga
     3361 ctagatggag gaaatggatt tacaggggca aacataagtc ctcattatga tagtttattg
     3421 gttaagacaa tatcttggga tagaacattc caaggagcaa taaataaaac tataagatct
     3481 ataaaagaac ttagagttag aggtgttaaa actaatgtag gattcctagt taatgtttta
     3541 aataatccta tattctcaaa tggtaaatgt agtactaagt ttatagatga aaatccagat
     3601 ttatttgaaa taacagaaag taaagacaga ggaacaaaat tacttcaatt tataggagat
     3661 gtaattgtaa atgataatgc ttgtaaagaa aaaccattat ttgatgcatt acatgaccca
     3721 agaatggata aggatggttc aaaatcagaa ggaagtaaaa tacttttcga taagttaggt
     3781 aaatctgcat atatagaaaa gataaaaaat gataagaaat tactattaac agatacaaca
     3841 atgagagatg cacatcaatc attattagca acaagaataa gaacatatga cttattaaaa
     3901 gctgctaagc caacagaaaa atatcaaaag gatttattct cacttgaaat gtggggagga
     3961 gctacttatg atgttgcata tagattttta aaagaatctc cttggcgtag attacaaaag
     4021 ctaagagaag aaataccaag cataatgttc caaatgttgc ttagagcatc aaatggagtt
     4081 ggatataaaa actatccaga taatgtaata gaagaattta caaaagaatc tgctagacaa
     4141 ggtatagatg tatttagaat atttgattca ttaaactggg ttgaaaatat gaaaccatct
     4201 attaatacag ctttagaaac tggaaaaatt gttgaagcaa ctatgtgtta tacaggtgat
     4261 atactagata agactaaaac taaatacaat ctagaatatt atataaaaat ggcacaagaa
     4321 ttagaaagct tgggagcaga cataatagca ataaaagata tgtcaggtct tcttaaacca
     4381 tattcagctt acacattagt taaagagtta aagaaaaatg taaaagcacc aatacattta
     4441 cacactcatg atacaagtgg aaatggtgta gcaacttgtt taatggcgtc agaagctgga
     4501 gtagacataa ttgatgctgc attagaatct atggcaggac ttacaagtca gccatcactt
     4561 aatgctatag tagaggcact taagaataca gagagagata caggaataga tttatttgga
     4621 tatgatgagt taggaaaata ttataaggat ttaagaaaag tttataataa atttgaaagt
     4681 gatttaacta attcttgtgc agaaatatat aattttgaaa taccaggagg ccaatataca
     4741 aacttaaaac ctcaagctga tagtttagga ttagttaata gatttgatga agttaaagaa
     4801 aaatataaag aagctaatga agttgtagga gatataataa aagtaactcc atcttcaaaa
     4861 gttgtaggag acttagctat ctttatgact aaaaataaat tagataaaga caatataata
     4921 gaagaaggta aaaatttatc attcccggat tctgttgtgg attactgtaa aggtatgatt
     4981 ggtcaaccag aaggtggaat accaaaagat ttacaagagg tagttctaaa aggtgaagaa
     5041 gctataacag taagaccagg ttcattatta ccagcagaag attttgatga aatagctaaa
     5101 tatctaaatg aaaagtatga tataaatgct aatataagaa atgttataag ttatgcttta
     5161 tatccaaaag tatatgaaga ttatatcaaa catctacaac attataatga tatatctaaa
     5221 cttgaaagtg atgtattctt ctatggatta aataaaaatg aagaatgtga agtagaaata
     5281 gaagaaggta aggtattaac aataagatta gttgaaattg gtgaagttaa ggaaaatgga
     5341 tttagaacta taggatttga gttaaatgga atggttagag aagtagaaat aaaagataag
     5401 aatttctctg gaaaaattaa taatgttgaa aaagctgata tgaatgaccc tcttcaaatt
     5461 ggagcaagca taccaggaaa agttataaaa ataatggtta aagaagaaga tgaagtaaaa
     5521 gcaaatcaac cattaatcgt tatagaggct atgaagatgg aaactataat agttgctaag
     5581 actgatggag taattaagtc aatcaaagta aaagaagatg atatggttga agataaacaa
     5641 ttattaatga taatgaagta atttaattaa aataaattaa aaagcactct gaaatgttta
     5701 ataaaaaatg ttttagagtg tttttattta ttttaattaa atatttgtta aaaaaaagag
     5761 gacaattacc tccagatgta gaaataaagc tagtaaggaa aatgttatct aaaaattcta
     5821 aatatagtaa atattttcca taatatttct taggaaataa cagcaaagat aaaatacata
     5881 aatttaaaag gtgggtgctt atatgaaggt ttatgatagt aaaatgttga gaaatgtagc
     5941 ggtattagga catagcggat gtggaaaaac aaatctaatc gaaactatag cttacacagc
     6001 aaacactaat aagataccta aattaacaga caaggtaaat atgacttata gcatgggatt
     6061 aatacctata gagtataatg attataagtt taacttattg gataccccag gatattttga
     6121 ctttagcgga gacgttgtgt catctcttag agcaagtgat gcagcaataa tagtaataga
     6181 tgcaacagcg cctattcaag ttggtacaga gaaatcatta gaactaactg agagtattcc
     6241 taagataatg tttataaata aaatagataa tgaaaaggca agatacaaag atgcaatagc
     6301 aatgttaaga gaaaaataca ataataagat agttcctatg ataagtccta tatacaaaga
     6361 taaaaacttt gtaaagttac ataatgtttt tgaaaacata gatgatttag aaggtgaatt
     6421 taaggaacaa gctatgagtg ttaaagaagc attaatggaa cttatagcag aaactgatga
     6481 ccaaatatta gataaatatt ttaatggaga agaactaact acagaagaga tacaaaaagg
     6541 tattattata ggtatacaaa gaggtgatat tatacctgta atatgtggtt caactataaa
     6601 taatataggc acaaaggaaa tattagatac aatatcaagt tatttagaac ctatctttac
     6661 agaggaaagc aaaccattta ggggattagt atttaaaact atggttgacc catttgttgg
     6721 aaagatgtca tatataaaaa ttactgaggg tgtactttct aaggataaag atgtttttaa
     6781 tataaataaa aatgtaaaag aaaaaatagc taatatatat acattgagaa atagtgaatt
     6841 agtagaaata gaaaaagcta aagctggtga tatagtagtt ataactaaag ttaattctct
     6901 aaaaacagga gacactatat ctgcagataa agatgcagaa gctttagaaa agatagattt
     6961 tcctaaacca caaatatatt atgcagtaac tcctaagaat aaaggtgatg aagaaaaagt
     7021 agcatctgta ttaaataaac tagtagaaga ggaccctaca ttacattggt atagaaatac
     7081 agagacaaaa caagcacttt taggaggtca aggagaactt catataaaaa caattaaaaa
     7141 taaaatgaaa gataagtttg gtgttgatgt tgaattgaat gaccttaaag ttccttatag
     7201 agaaactata aaaggaacag ctgatgtaca aggaaaacat aaaaagcaat caggtggtca
     7261 tggtcaatat ggagacgtaa aaataagatt tgaaagatgt gaatctgatt ttgaatttac
     7321 agaagaaata tttggtggtt ctgtaccaaa acaatatata ccagcagttg aaaaaggttt
     7381 aaaagactct atgcaaaaag gtatattagc aggatatcct gttacaaaca taaaagctac
     7441 gctttatgat ggttcatacc atgatgtaga ctcttctgaa atggccttta agatggcagc
     7501 aagtgcagcc tttaaaaaag gtatggaaga agcacaccca atactactag aacctataat
     7561 gaaacttaag ataactgttc cagaagaata tatgggagat gtcatgggtg atataaataa
     7621 gagaagagga aaaatatttg gaatggaacc agatgacaaa gggaaacaaa taatatttgc
     7681 agaagctcca caagcagaga catttaaata tgcaatagat ttaagagcaa tgacacaagg
     7741 tagaggatac tttgaaatgg aattagagag atatggagag gttccatcac agtttgcaga
     7801 aaaaataatt gggttagcta ctgcaaagta atgaacaaaa taaatagaaa aataattaat
     7861 atatattatt aaaaatattt ttagggtttt aactaggtat aggtttatgt taggtaaact
     7921 tatacctagt ttttatacat ttttgatgaa taaaaaaata tataatactt tcttaaatat
     7981 tttaataaaa agaaagtatt tttttattat tatttttaca aatgtttagt aataaagatg
     8041 agaaggtata tttaaaatat aaagtattta aagttctgaa atctaatcat gaagcaaaaa
     8101 attagcattt ggttatcaat aaatataata aataaaaact cataattaat gaaagaataa
     8161 attcatatct aaatttctat aaaaaattta aatttataca atttagggct ttaaaaattt
     8221 aaataatagg tgtaaaataa tagtcaaaga aagtcaaaaa taaaaaggtg tgtggtgata
     8281 ttaatggcaa ctatgacaga tataattgaa aaatttatta aagatttgat ggaagaagat
     8341 aatagcatac aaattcaaag aaatgaacta gcaaacttgt ttagttgtgc accatcacaa
     8401 ataaattatg tcttaacaac aagatttaca atagatagag gatattacat tgaaagtaaa
     8461 aaaggcggcg gaggatatgt tcaaatagaa aaaatacgaa aaagtaaaga tgggcatatt
     8521 agagaactac taaatgaaaa aataggaagt cagatatcct ataaaaaagc aaaagaatta
     8581 ttagaaggtt taaaagagtc ggatttgatt aacgaaagag aattaaaatt aatattatat
     8641 gcaatagatg ataaatcatt gtgtatgcca atatatgaat taaaagagaa ggtaaggtct
     8701 aatattttaa agaatataat aataggatta tttagtatag aggagtgata atttatgcta
     8761 tgtcaaaaat gcaataagaa taaagcatct gtatattata ataagatagt caatggagaa
     8821 aaaacagaga tgtatttatg tagtgagtgt gcaaaagaga atacagaaat gaattttaac
     8881 ttagatatgc ctttttcaat gatggatata ttttctaatt taggttttca acctaaaaaa
     8941 gaacttgagg aaaagctagt ttgtcctaaa tgcaatacaa catatagtga gtttaagaat
     9001 aatgggagat ttggatgtag tgaatgttat aatgcattta gtagtcaagt aaatccaatg
     9061 cttcaaaata tacatgggca tatagaacat acaggaaaag cacctaaaaa atctttttat
     9121 aagataagtg tagaaaatga gattaaagaa ctaaaagaag atttagatag agctataaaa
     9181 aatgaagagt atgagttagc agctcaattt agagataaaa ttaaatattt aaaaggttct
     9241 attgactaag attggagtga tattatgaaa gagaacatag tcatgaaatc aagagttagg
     9301 ctagctagaa atttaaataa ctatccattt ccaaataaat tagataaaga atgtgccatg
     9361 gaaataatag aaaaagtaaa aaatgcgttt attaattcta acttagaaca aaaagaagaa
     9421 tttgatttct ataaaattga agatttagac caatctaaaa aaatgcttat ggttgaagaa
     9481 catataataa gcccagattt agcagaaaat gataaaagtg cagttatagt aaaaaaagat
     9541 aaaactataa gtataatgat aaatgaagaa gaccatataa ggatacaaac tatatgtgat
     9601 gatttaaact tagaatatgc atatagtgta gcaaatgaaa tagatgattt gttagaatca
     9661 agtttagaat atgcctttaa cacaaagcta ggttatctaa catcttgtcc aactaataca
     9721 ggaacaggaa tgagagcttc tgtaatgatg catttaccag cattaagtca actaggctat
     9781 atggatgaac tttataaaat ttcatcacaa ataggcatag caattagagg gatatatgga
     9841 gaacgaacag aagcattggg aaatatatat caaatatcta atcaacttac acttggaaga
     9901 acagagtcta atattataga aaatgtaagt ggacttacaa aagatgcaat ttcaaaagaa
     9961 ataaaagcta gagaaatatt gcaaaaaaaa ttaggtataa aactagaaga taaaatattt
    10021 agatcgatag ggacacttga aaattcaaga gtaatgagta gcgcagaggc aatgagccat
    10081 ctatcaaata taaaaatggg aatagaaatg aactatatag ataaactaga tttaagggct
    10141 atagaacaac ttatgatagg tatacaacca gcgcatcagt caataatgta taagagtgat
    10201 gatgttgaga atagagatat aaatagagca acatatatta gagaaacttt ggaaaaatta
    10261 agaggaggga tgaattgtga acttcaatag atttacacaa agagctaaaa aggcaattga
    10321 tttagcgttt gaatctgcta aaagtctagg acacaatatt gtcggaagtg aacatatact
    10381 tttaggtctt ttaagagaag aagaaggtat agctgctaaa gttctaagta aagttggatt
    10441 tacagaagct tatttagaag gcaaaatagt tgatatggaa ggtaagggag aagaaatctc
    10501 agaagatata gtattaagtc caaggagtaa acaaatactt gaattatcag gaatgtttgc
    10561 aaataaatta aaaacaaatt atattgggac tgagcatata ttattagcaa ttattcaaga
    10621 aggtgaagga atagctaata agattttaaa ttatgctgga gtaaatgata ggactttagc
    10681 ccaattaaca attgatatga tgggtattag tgataaaaat caatacaaag cagagaatag
    10741 ttacacgggc aaccagaatc aagcagaatc taaagtactt gataagtatg gaagaaatct
    10801 tacactatat gcaaaacaga ataaaataga ccctgtaata ggtagagaaa aagaaataca
    10861 aagagtaata caaatattaa gtagaagaac taaaaacaat ccagtgttga taggagaccc
    10921 aggtgtaggg aaaacagcca tagctgaagg tctggctaca aatatagcct taggaaatgt
    10981 tccagaaaca ctaaaaaaca aaactttata ctctttagag atgggttcat tattggctgg
    11041 agctaagtat agaggagaat ttgaagaaag aattaaagaa gttgtagatg aagttgttaa
    11101 aaatggaaac ataatcttat ttatagatga gatgcacaca ataatagggg ctgggtctac
    11161 tggagaaggc tctatagatg cttcaaatat attaaagcca gccttagcaa gaggagaaat
    11221 acaagttata ggtgcaacta caattgatga atatagaaag catgttgaaa aagattctgc
    11281 tctagaaaga agattccaac cagttatggt agatgaacca actaaagagg actcaataaa
    11341 aatattagaa ggattaagag ataaatatga agctcatcat aaagttaaaa taactgatga
    11401 tgctataaag gcagctgtag aattatcaac tagatatata tcagacagat atctgccaga
    11461 taaagctata gacttgattg atgaagctgc atcaaaagta agattaaaag aaaatacacc
    11521 tccttcagaa ataaaaaaat tagaactaga aatagaaaat atagataaag aaaaagaaga
    11581 agcagtaaga tgtcaagact ttgagaaagc agccaaaata agagatgaac aaggtctact
    11641 taaaaaacaa ttagaagatg ttagagaaag atggaacaag tcatctaaac attcagattt
    11701 agttgatgga gaagttattg ctgaagtagt gggcttatgg acaggaatac cagttaataa
    11761 aattcttgaa gaagaggctg atagactttt gagacttgag gaaatactgc acaatagagt
    11821 tataggtcaa gaacaagcag taaaatctat ttcaaaggca atcagaaggt caagagcagg
    11881 tcttaaagac cctaatagac caattggttc atttttattt ttaggtccaa caggagtagg
    11941 taaaacagaa ttatctaagg cattagcaga agtgcaattt ggagatgaaa atcaaataat
    12001 tagaatagat atgtctgaat atatggaaaa acatgctgtg tcaagaatga taggttcacc
    12061 tccaggttat gtaggtcatg atgaaggtgg tcaattaact gaaaaagtaa gaagaaatcc
    12121 atattcagtt atcttatttg atgagataga aaaagctcat ccagatgtat ttaatattct
    12181 gttacaaatt ctagatgatg gtagactaac tgattcaaaa ggaagaactg tagattttaa
    12241 gaatactatt gtaataatga catcaaatgt tggagcatct acaattggta gacaaaaaac
    12301 tttaggattt agtatagcta aaggagacga agaagaaaaa tctcaatatg aaaaaatgaa
    12361 agaaaatata atgggtgaat taaaacaaag attcagacca gagtttttaa acagaataga
    12421 tgatataatt gttttccact cactaaatga aaatcatata tcaaaaatag tacttttaat
    12481 ggcagcgaaa ctacaagaga gattaaaaga aatggatata aaattagaaa tgagtgatga
    12541 ggctgttaaa ttaatttcta aatctggatt tgacttagaa tatggagcaa gacctcttaa
    12601 aagagcttta caaaaagagt tggaagatga gctatctgaa gcaatcttaa aaggtaatgt
    12661 gaaaaaggga agtaatgtag tagcaaaagt caaagatgaa aaaatagttt ttgaaactaa
    12721 ataatttaat agagttgcat tttaaaatat tgcaaatata agttttataa tctaaacaaa
    12781 attttaaggg caatgaatct ataataattc attgtcctta attattttat ttaatcttta
    12841 catattgtct ttcagatatg ctaaaattat agtatagaat aaaaaattaa ggatgataaa
    12901 agtatggcaa aaataaaaac aaaatatgta tgtcaatctt gcggatatga aacagctaaa
    12961 tggcttggta aatgtccaga atgtacaaaa tggaatacat ttgtagaaga aatagaccaa
    13021 aaaagcacca aaaaagaggt ctttataata gataaatctt catctaaacc agtcagtata
    13081 aactcaatag agagtaaaga agaagagcgg tttacaacag atataaatga attagataga
    13141 gtgcttggag gaggtatagt taaaggctct ttagtgcttg ttggtggtga ccctggtata
    13201 ggtaaatcaa cacttttaat ccaagtatct agtaatgttg ccaatttagg aaaaactgtt
    13261 ttatatataa ctggagaaga atcagaatct caaataaaaa tgagagctaa aagattaggt
    13321 attaactcag aaaatttata tatatttgct gaaaataatt taagtataat tgaatcttat
    13381 ttagaaagtg taaatccaga attaataatt ctagactcaa ttcaaactgt cttcagtcca
    13441 gaaatatcct cggcaccagg aactgtcagc caaattaaag aaggtacttc aaaattcatg
    13501 aaaatttcta aaaaaatggg aatttcaacg ttcatagttg ggcatgttac aaaagaaggt
    13561 tcactagcag gaccaaaatt attagagcat atggttgata ctgttctata ttttgaagga
    13621 gaaagatata atacttatag attagtaaga gctgttaaaa atcgatttgg ttcaactaat
    13681 gaacttggag tgtttgaaat gagggattta ggattagtag aacttgataa tccatctaaa
    13741 attttaatct ctgaaaaacc aaaagatgta gcaggttcag taataatatc tactgtagaa
    13801 ggtacgagac caatgttact tgaattgcaa gcattagttt ctcctacaag ttttggtata
    13861 ccaaaaagaa catctacagg agtcgattac aatagagtag gtatgttatt agcagtatta
    13921 gaaaaacgtg ttggtttgca aatacaaaat caagatgtct atataaacat agttggagga
    13981 ataaagataa atgaaccctc aattgattta gggatagcca tttctgtagc atctagtttt
    14041 agaaatatac cgatagatga agatatagct gttacaggag aagtgggact tacaggagaa
    14101 gttagagcag taagctttat agaaaaaaga atagctgagt gtaaaaaact tggatttaaa
    14161 aagatagtag ttcctagaag taattatgat gttgtaaaag agaccaaagg catagaaata
    14221 tggccagtag ataacttaag acaagctata aatatagtac ttgggaggaa tcaataaata
    14281 tggagaattt tctagataat aaaaatatgc tatatgcatt aaaaatgata tctcctggaa
    14341 ctccacttag attaggtcta aacaatgtac taagagctaa gactggtgga ttaattgtaa
    14401 ttgcaacaaa cgaagatgta atgaaaatag tagatggagg atttgctata aatgcagaat
    14461 attcaccatc atatctatat gaattagcta aaatggatgg agctatagtt ttaagtggtg
    14521 atgtaaagaa aatattattt gctaatgcac aacttatacc tgactatttt atagaaacat
    14581 cagagacagg aacaagacat agaacagcag aaagagtagc aaaacaaact ggtgctatag
    14641 tcataggaat ttcacaaaga agaaatgtta taacagttta tagaggaaat gagaagtatg
    14701 tagtcgaaga tatatctaag atatttacta aggcaaatca ggctatacaa actctggaaa
    14761 aatataagac agtattggac caagctgtaa caaatttaaa tgccttagag tttaatgatt
    14821 tggtaactat ttatgatgtt gcattagtca tgcaaaagat ggaaatggta atgagagtta
    14881 caagtataat tgaaaaatat gtgatagaat tgggtgatga aggaacttta gtaagtatgc
    14941 aattagaaga attaatgggt acaaccagaa tagaccagaa attaatattc aaagattata
    15001 ataaagaaaa cacagaaata aaagaactta tgaaaaaggt caaaaattta aattcagaag
    15061 aactaataga attggttaat atggcaaaac tattagggta tagtggtttt tcagaaagta
    15121 tggatatgcc tataaaaaca agaggttata ggattcttag caaaatacat agactaccaa
    15181 cagcaataat agaaaactta gtaaattatt ttgaaaactt tcaacaaatt ttagatgcat
    15241 ctattgaaga attagatgag gttgaaggaa taggtgaaat aagagcaaca tatataaaaa
    15301 atggactcat aaaaatgaaa caattagtct tattagatag acacatatga tgaattttaa
    15361 atgaatttta ctactttttt cttgaatata atatacactt tggtctataa tagataagta
    15421 taactagggg gtgaagcatt gataaggaaa gtaacaagaa tattgtttat tttacttgga
    15481 tttacaatag gtataacaac ctatttaaca ttaatgaaag attttgaaat attaacattt
    15541 ggaaaagaaa catatggtta tatcgcagct gtagtagcag gtataataat agccatttta
    15601 ggatatctta tagaaccatg ggttgtaaat aaagtaaaag aaattgctaa aatagtggat
    15661 aaagaattgt caaaatatcc tcagactgat atattgttag ggtctatggg tcttatagta
    15721 ggatttgtta ttgcgtattt attaagtgga ttagtaaata gaataccaat agtaggtgga
    15781 atactatcct tactattata tttattccta ggatatcttg gaatgaaagt tgcgcttaaa
    15841 agtaaaaatg atttatttaa tattggaaaa ctaggaagat tagcaaatcc tattaaggat
    15901 aaagataagg ataaagaaaa taaaaaagaa gtaaaagcca ttcctccaaa agtattagat
    15961 acaagtgtaa taattgatgg aagaatagct gatatttgta agactgggtt tatagaagga
    16021 aaacttatta ttcctgcttt tgttttggag gaactaagac atattgcaga ttcttcagat
    16081 gatttaaaaa gagtaagagg tagaagaggt ctggatatat taaatataat tcagaaagaa
    16141 ttaaatatag aagttgaaat aagtgagaga gactttgatg atatagcaga agttgatagt
    16201 aaattattaa agcttgctca ggttttaaat ggaaaagttg ttacaaatga ttataactta
    16261 aataaagttg ctcaattcca aggtgttgaa gtattaaata taaatgagtt agctaatgct
    16321 ataaaaccag ttgctatacc aggtgaagat atggttgttc aagtggttaa agaaggaaaa
    16381 gaggctcaac aaggtgttgc atatctagat gatggaacta tgatagttgt tgatggagga
    16441 agaaaatata tgaatgagac tataaaagta ctcgttactt ctgttcttca aactcctgca
    16501 ggtagaatga tatttgcaaa acctaaaaat taatatgttc aaagaaacct ataatttggg
    16561 ctgtttattt taaatagatg gtccaaattt atttatttat aacgataatg tttaatgtat
    16621 tcttaaagat ggggatttaa atcagaaagc agggagaatt tatgagaaaa aagattatat
    16681 ctttcttagc aacatttatc ataatattaa catcagcaag tcagtatagt tttgcagatg
    16741 atatatcaac acgtggaaaa gtaatattta tagatatgaa tagaactagt atgagtaata
    16801 tgttaagaat aaaatcatta agagaagaac tagacaatag agggtatatt ggcttgatga
    16861 atataagagg cgataaagga agtgatgata gaaggtcata tgctagtatg ggtgctggtg
    16921 gtagagctaa tgtggctaat gaagaagata ttaattttga aagctcgagt aaggatagaa
    16981 atatagtttt tgaatcagct acaggaaaaa gtgcaaaagg tataaataat ttaactataa
    17041 ataagtctat aaatgaaaat ttaaactttg gagagtatgg ttctgtatta gggtcattag
    17101 gacaaagttt atctgataat ggattaaaag catctgtatt gggtaattca gatataattg
    17161 aaaatggtca acttataaaa aatagaaact tatgtctaac tgcaatggat gaatatggaa
    17221 gaatacctaa tggaaatgta gatactataa ataaaaaaga cttatctatg ccttatggaa
    17281 tatcaactga ttatgataaa ttgattgtag agactaaaga agcttacaaa aataatgatg
    17341 tgatttttgt ggagcttgga gacacatata gattagattt atataaacca aatttaaatg
    17401 aaaaaacata tgagagtatg aaagatagca tagaaaaaaa tatagatata tatttaaaaa
    17461 atatattttc tatggttgaa gaaaatgaca ctgtctacat agcaagtgca tttccaagtg
    17521 atttagatta taaaaataaa agaagacttt ctccaataat aaaattaaat ggagaaggga
    17581 aaggtatatt atcatcttca actactagaa gagaaggtat tgtaacaaat ttagatgttg
    17641 gggttgaaat attagacaac tttaatataa aaaatcaaaa tatggttgga agaaaatatg
    17701 aacttattaa cagagatgat aataaagaat ttttgatgga tgaatatcaa aagatagtat
    17761 ctatttcaag cataaggtct acaatactga atggttttgt aagtatagtt ttcttgtcat
    17821 ggatagttgc tatgattgca attttattta gaaagcatat atcaaaaaat tataaagaaa
    17881 caacattttt tatattaaag gaacttttaa aaataggaat agtaatgcca ctgtcattta
    17941 tgataacacc aataatgaat ttcaaaacac cattagcaat atctttagga ataattataa
    18001 taacacttac tatatatctt ataagtaaag ttttaataaa gaacgattta aaaaacatgc
    18061 tgttttttac tggattaacc atagtaatta tggttattga tgctgggttt ggctcatatt
    18121 taatgaaaag caatgttatg agttatgatt gtataattgg agctagatat tatggagtag
    18181 gaaatgagta tcaaggtgtg gcaatcggta gtgcaatatt tacatttgca attttactta
    18241 catataaaaa tataccaaag tggtctataa tagttttttc tttggttatc ttaattacaa
    18301 gtgcatctcc aattatggga gcaaatgttg gaagtgctat ttctgaatgt gttgcatttt
    18361 tactatttat actattgata tataatgtaa agatagattt caaaaaaatc gtattattag
    18421 gtatagcagt agtatttgta ttaggagttt ttgtagcaat agatatgatt ttgggaagta
    18481 attcacattt aggcatgttt gtaaaagaaa tttactttaa tggaccagga gaaataatac
    18541 aaacatttag tagaaagata gagatgaatt taaagctagc tcaaaccagt gcatgggtaa
    18601 atatactgtt aactggaata ggtgtaattt tagttcttat gattaatcag attagatact
    18661 ttaagcaact gatggatgag tatccaattg tatttaaagg atttatagca tcaatagcag
    18721 gatgtttagt aactctttta gtgaatgact ctgggattgt gtcatcagct acagccttta
    18781 tttatgtaat tgtacctatg ataacattaa gtataaatct cacagcatta aaagaataag
    18841 tactgtatga ataa
//
LOCUS       DAELNY010000066        18273 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.066, whole genome shotgun sequence.
ACCESSION   DAELNY010000066 DAELNY010000000
VERSION     DAELNY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.066"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(202..882)
                     /locus_tag="KPE55_001939"
     CDS             complement(202..882)
                     /locus_tag="KPE55_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493007.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(932..2947)
                     /locus_tag="KPE55_001940"
     CDS             complement(932..2947)
                     /locus_tag="KPE55_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0493008.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGQLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(3019..3696)
                     /locus_tag="KPE55_001941"
     CDS             complement(3019..3696)
                     /locus_tag="KPE55_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493009.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4276..4359)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4592..5434)
                     /locus_tag="KPE55_001942"
     CDS             complement(4592..5434)
                     /locus_tag="KPE55_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0493010.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5686..7470)
                     /gene="pepF"
                     /locus_tag="KPE55_001943"
     CDS             complement(5686..7470)
                     /gene="pepF"
                     /locus_tag="KPE55_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0493011.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7536..8201)
                     /locus_tag="KPE55_001944"
     CDS             complement(7536..8201)
                     /locus_tag="KPE55_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0493012.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8352..8513)
                     /locus_tag="KPE55_001945"
     CDS             complement(8352..8513)
                     /locus_tag="KPE55_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG0493013.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8591..9100)
                     /locus_tag="KPE55_001946"
     CDS             complement(8591..9100)
                     /locus_tag="KPE55_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG0493014.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9306..9524
                     /locus_tag="KPE55_001947"
     CDS             9306..9524
                     /locus_tag="KPE55_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0493015.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9542..11488
                     /gene="feoB"
                     /locus_tag="KPE55_001948"
     CDS             9542..11488
                     /gene="feoB"
                     /locus_tag="KPE55_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0493016.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11667..12722)
                     /locus_tag="KPE55_001949"
     CDS             complement(11667..12722)
                     /locus_tag="KPE55_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0493017.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12770..13570)
                     /locus_tag="KPE55_001950"
     CDS             complement(12770..13570)
                     /locus_tag="KPE55_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0493018.1"
                     /translation="MNKLTKKELRSMFWRSFVLQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13563..14354)
                     /locus_tag="KPE55_001951"
     CDS             complement(13563..14354)
                     /locus_tag="KPE55_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0493019.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14394..14816)
                     /locus_tag="KPE55_001952"
     CDS             complement(14394..14816)
                     /locus_tag="KPE55_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG0493020.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14832..15311)
                     /locus_tag="KPE55_001953"
     CDS             complement(14832..15311)
                     /locus_tag="KPE55_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493021.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15443..18199)
                     /locus_tag="KPE55_001954"
     CDS             complement(15443..18199)
                     /locus_tag="KPE55_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0493022.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYENSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIFEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         5971 a         3137 c         2016 g         7149 t
ORIGIN      
        1 tatcttttca aatataatct ataaaataat agttccgtta cgcatttttt tatataataa
       61 aactgactgt aatttaactg gcaaagtcta gaagaatagg ctttatcaat taaattacag
      121 tcagctattt tataataatt tagtttagat atcgttagca tttcacattt ataacaatta
      181 ttaatgatat taatatattt attattcctt attaaaataa tatcctgctc ctcttattgt
      241 tataattttc tgaggatttt tataatcttt ctctattttc tttcttaatc cattcactgc
      301 aactaccaca ctatagttat cctctaagta ttcattcatc catacatgtt catatatctc
      361 tttagtagtc aaagtctttt caggattctc tataaaaaag tttaacaact tattttctgt
      421 tggagtcaat tttattattt tatcttctaa ctttattact ccactagttc tattatattc
      481 ctctaatttt gtattatttt cattattatt tacatatgag ttcattcttc ttaaaatagc
      541 ttttactttt gcatataata tttctaaatt tagaggcttt attaagtaat cgtctccacc
      601 agaatttaaa gcatttaaaa tgctttggtc ttcatttata taagtcatat aaacaattgg
      661 acaagtagtt acatttctaa tttttttaca aagagtccat ccatcaccat cacttagaat
      721 tatttctaaa aatattaaat catatttatt gctaaatatt tttccaatag cttcttcaca
      781 attatatgct aaatctatac ttattccttt ttcctccaga aatcctttta gcttctggca
      841 attatccttg tctccatcta ttatcaatat cttagtttgc attataatca ctcccaaata
      901 tcattattct tttatattaa ataagctatt attataaaaa acatagactt aattttattg
      961 taaaaacagt tccttttcct agttctgaat ctacaaatat ttctccacca tgctctctta
     1021 taatggattt gacaatatat agccctaacc cttgcccttt tggctcatta ttgtctcttt
     1081 ctgtaaagaa tctgtcaaat acctttggta tattttctgg aggtatacct tttccattat
     1141 cagaaatttt aataattgca aatccatctt ctttatccat tgataaaatt atctttccat
     1201 caaatggagt aaaggtcaaa gcattataaa ttaaattctc gaatgctctt gtaagcatct
     1261 ctttatttat ctttgcatat atttttttct ccaatacgta agcttcaaag tttacaccac
     1321 atgcttgtgt atctggcaaa ttattttcat atatctcttt aataaaatta ttcagacata
     1381 ttttttcact aataccaatg cttttatctt gtgatgtaaa ttcttgcaaa ctacgtactc
     1441 tattttgtac ttcatttgac ttttgattta tagcataaag ataggttctt gtctcttcat
     1501 ctacatgaat attaccaacc tttatcaaat ctataaatgc ttgtatagcc gaaacaggtg
     1561 cctttaaatc atgtgctaca tctgccaaga atcttttacg ttcatttaac aatgttgtaa
     1621 gttcattagt tctcacttca acttcatgtt caagattcct tgtaagtttt tcattctctt
     1681 ttattattat tctattcctg tttatcatca taacagaaaa taaaataaca attacaaacc
     1741 cacaatattc tgtttgccac cctgtaaata taggctcaaa tctattactc gtaactgcat
     1801 caaataatat gctaattcca aaaacagtgt ttactgaaac taaaatataa gaacttatgt
     1861 gctcagagca tgtcttttta tatattgaaa ccagcgaaga cattaatata tatataaata
     1921 caatatattt atatatgtct attaacttcc catatatgtt gacaaagtac atatcatatg
     1981 gaagaagaat aattggtatc actatagata tgatactcat tgtaattcct aatggtaaaa
     2041 ttaaaaatct atacacttta ttttcttcta ttttggatat acttccattt atacaaataa
     2101 tacataaaac taccataaaa tatgacaaat cttcaaccgc atatgtaaag cttactaaag
     2161 gcagtcctaa caaatgtaca aagggatagg atacatggat ggcaaagaaa acacacatac
     2221 atccaaaata taagtaaatt ttacttttgt ttgaaagaaa ccatacagac aatgaaaata
     2281 tagctattgc aaaagttgtg aagcaaatta tactataaaa aagtaatcga taaaacatca
     2341 tattacttat gtcctttgtt tctcctaatg caggcggata atacaaacca ctataataat
     2401 gtgaaaaatt cgatacattg attataattt ctgtattttt ctttgcaata aaacttacta
     2461 ctgaattttt aattttgggt ctatattttt ctgttcctac atctcctaat ttagaaacca
     2521 tttctccatt tatccatact tcacttgcgg aaaaaatctc aggaagctct aaacttagta
     2581 gcttatcctt gccttcattt tttattctca tacggtaagt agcattccca tatggtgact
     2641 gattcccttt tctcatagaa aaatctggat attgacctat aaaaacatat tctggtttta
     2701 tatcttgctc tgtaaaatca ttcggtttaa atattttatg attataaaac tcccagtcat
     2761 ctatcaaaaa gacaagttgc cctttagtga aagtatcatt atctatagtt aaaattccat
     2821 tacttgctct aatagactta tatgtatatt tattatcata tttatacaga agatttaatc
     2881 ctgttattgc caacagagag actatcaata caatgagagt tccttttagt atattatact
     2941 ttttcattgc atcaaccaca tctctttcat agtcgtatta aataaaacaa gaactattta
     3001 ttaattacag tatctcactt atggtacaaa cttatatcct tttccccata cggtttgaat
     3061 gtattgatgc tccctgctta aagaattaat tttttttctc atctgtgcaa tatgaacctg
     3121 cactgtatgt tgatctccaa tatcaggcat tttccaaact tggttatata tctctatctt
     3181 tgaaaatact tgttctttgt tctcagctag aaacaataat atttcaaatt ccatagtagt
     3241 taaatctata ctttctccat caaaagttac ttttcttgaa tttacattta ttactaacgc
     3301 tgaaaacttt aacacattag gaactgatgt aatatttttg tactgcctca tacgagcata
     3361 tattctaagt tcaagttctt ttaaactata aggttttgtt atataatcat cccccccagc
     3421 taaaaatccc cgaacttttt cttcttcatg ctcataatta gataaaaata ttataggtat
     3481 gttaaccttt tcttttatat cattgcacat tttatatcca tctatatctg gcaatgaaat
     3541 atccaaaatt atacagtcta aaactatact ctctactata tccatagcat ctttagcagt
     3601 ctctgatgta tatacattaa aacctatatt agaaaaataa atttgattta tgtttaaaat
     3661 atcaacatca tcatctacaa ccagtaaatt atacattaca ccactccatt caaaggtatt
     3721 ttaattataa ttccaagttt tatagttatt acaaattata atgttttatt ttcatatata
     3781 actggtatta ttattttata atatctttac aaactagact ataatactta agtatttttg
     3841 aataattctt gaatattgta ttaaacatta agattcatta ttaaatacta attaaagtct
     3901 ccgattgttg gagaatggag cttaaagtca ttttcacgta taaatttatt atttttctag
     3961 aaacttgtct attggctagt ttgagtattg atttatatga tttatatggt tataatataa
     4021 aataaattgt attattttgg taaatatatt gttacaaaag gtttatattc tgcatctcaa
     4081 tgtttataaa aactttattt tcttattata atactattat tcttttaaaa aaatttccct
     4141 tttccaacaa atggctactt taaaagccaa aattttacct atcaataaaa atcttaacga
     4201 ctaatactct tattttcaaa ttttgcaaca tttttgttaa ttttaaaaca aaaaaagact
     4261 atgcaatata aaaattatat tcatagccgg ttgcaccact aactccatat atctccagat
     4321 gcccaataat aagatatact tctcagtttc taaaatattt ttattaaaaa tcttttatat
     4381 attttcataa atttcttttt tattctaaca ttgtaatatt attttgtcaa tatttccata
     4441 ttcttagata taagatacta aaatacctat tttatctaaa gatatttata tttctacatt
     4501 caataataga caataccctg caagttaaag catcattttt atgagcttat ctcacagggt
     4561 attatatgtt tactttatat tataactctt gttattgatt aactttaata aatccttttt
     4621 ctgaaacaag tgcttgtcct tcatcactta atatataatc tattaaagct ttaccttttt
     4681 ctgacaaacc atcttcttta ttaactaata taaatggtct tgatattgga tatttattag
     4741 ctattacatt tgcatcagtt gcttcaacat catttatttt taaactattt actttctcat
     4801 caacatatcc aaatgatata tatcctatag cattttcatt tccttcaact gtagttttta
     4861 tatttcctga accatcactt atttgtgcat ctttagttag ttcttctgaa tcaaacccta
     4921 caatttcttg gaatccatct ctagttcctg aaccttcttc tcttgatact attactattg
     4981 gtgcatcatt tcctcctact tctttccagt tagtaatctt tcctgtatat atacctttaa
     5041 cttggtccat tgtcagattt tttactgagt ttgcagtatt agttatcaat gctataccat
     5101 ctattgctat tttagtttcc tttaatccag cacttttttc ttcatctttt aaatctcttg
     5161 aactcattcc tatttcagat gttccttcta tagcattttt tatacctcca gaagaaccaa
     5221 tctgttgtac ttctacacta acaccttgat ttttttcttg aaacttttct ccgatagttt
     5281 ccataagagg acctattgaa gttgaacctg aaacagaaac tttgcttgaa tctcctgaat
     5341 cacctgaacc tcctgagtta gaactacatc ctactgctaa agctccaact aacattgtac
     5401 tagctaaaag aacgcctatt ttctttttaa acattttact tacccccgct tatattttaa
     5461 ttttttgttg tcatttttct atattttaat aatacagatt ctttgttaaa tgaatattag
     5521 agcagggtta agtaaatgta aatattgtta aatacatgta aaaacttaat tttatatgat
     5581 ttattcttaa ttagattaac tttttaaagt tactttaggc agatataaac ataatacaac
     5641 tcatattatt agaagtcttc attgaagcta tcaacctatc acatcctata attctttttc
     5701 tagtttatta actaattcag caaatacatt taaggcttcc tctatagatt cttttttcgt
     5761 catatctaca ccagcagatt ttaattgttc tagtgggtat tctgaccctc cactttttaa
     5821 gaagtttata tatctatcta ctgcagtact tccttctgaa agtatctgtt gacttaaagc
     5881 acttgcagca gaaaaccctg tggcatactt atatacatag aagtttgaat aaaaatgagg
     5941 tattctcgcc cattctattc ctatttgttt atctactatt gcagattttc cgtaatactt
     6001 ttcatttaat ttgtagtata tattagtaaa ttcatcagca gtaagtggtt ctccactctc
     6061 taccctttga tgtacaattt tttcaaattc agcaaacata gtttgtctat gaactgtagt
     6121 tctaaattgt tctaaataat aatttaataa gtatatagtt tcatccttag acttagaatt
     6181 ttctaataaa taatttataa gaagtaattc atttaatgta gatgcaactt ctgcaacgaa
     6241 tatcttatag tccgaatata aaaatggttg acttgttctt gaataatggc tgtgaacaga
     6301 gtgccctaat tcatgaatta aagtgaatag agaattaagg tcatttttat agctcattaa
     6361 tatataagga tgtgaatcat aacttcccca agaataagct cctccttttt taccttcatt
     6421 atcatataca tcaatccacc cttcatcaaa agcacttttt atgaccttca agtattcttc
     6481 tcctaaaggt ttaagtgcct ctaatatgat atcctgtgct ttatcatatg gtatatccat
     6541 gtcaaattta tctgtcaaag gaacatacaa atcatacatg tgtatttcat tcaatcctaa
     6601 aaatttcttt ttaagctcaa catatttatt tagattagga atacttttat ctatagctga
     6661 tataagatta ttgtaaacat ctaaacttac atcatctgaa aataaagacg cttctattgc
     6721 tgaattatac tttctagttt ttgcgtaaaa tatctctgat tttattccac cataaagcgt
     6781 tgaggcaaat gtatttttat atttctcata tgttgaaaat tcaccttcaa aagcgtcttt
     6841 tctaactcta ctattcttac tttttataaa taaagagtag tttgaatgag aaagcttaac
     6901 cttattcccc tcctcatctt ctatctcagg aaattccata tctgcatatg ctaacatatc
     6961 gtatacattt tctggaattg tcgataattc tgatgtagct gcaagtattt cttcctctcg
     7021 ttcactcaaa gtatgtggtt tatctctcaa tatatccttt atatattttg tataaaagga
     7081 tagcttctca tcttttaagt attcacttaa cttattctct tctatcgcca gtatttctgg
     7141 aactatataa gaagtagcca cacctaaatc agtagataac atttctgtct tagtggctct
     7201 tccttgatta tcattaattc tagtatcttc atgttgtttc atatgagtat atacatataa
     7261 attttgaaca atcctagatg cattttcaga gatatttaat actttatata agttctcctt
     7321 actctcagaa agcttccctt tatattcttt tacttcttgg ataagatttt ttactttact
     7381 tatgtctttt tcaatttcct ctttactgga gtacatctta tctatcttcc atttgtactt
     7441 ttcttctatt gtacttctat cagttcccat atcatacacc tctattttaa tttgttttat
     7501 attttattca ctttacaaaa tatattgtat atattttagt ccaaataatc tacatttatt
     7561 actccttcta tcatatttat ttctttcata acgtcgtgta agtgtattct cttagcataa
     7621 gatacaaaaa aattaacctt ccagtattct tcattacgac attctatttc catatttctt
     7681 acgataagtt gatttttttc aaatttatca tataaaaaag gtatagaatc caaattgctt
     7741 aattttaata tcaatacatt cttttcttgg ctaaataaaa acgtattgat tatcctaagc
     7801 acaaataatg ctatcattac aaataatcca cctagaattg ccaattcata ataaccatat
     7861 ccaactgcta taccaataca tgcagttgcc cacatcccag cagctgtagt aagccctttt
     7921 actccacctt tattttttaa aattgctcct gcacctaaaa aaccaattcc agataatact
     7981 tgagcaggca ttcttgctgg gtcagcattt gtatacaaat tataatcaaa gaataattga
     8041 atcgaagtta ttgaaacaat acaagaacct attgcaatta gtatatgtgt cctaaatcct
     8101 gcaaactgtc ttattttttc tctctctaag cctattattc ctcctattat tagtgctaat
     8161 agtaacctta ttgctatctc cttacttcct atatcaatca taatataaaa ccctcctgac
     8221 ttaattacaa cttatgtcct ccagataatt tacacatcag aaatactatt ttatatctta
     8281 atattattat taatatatgt aaaatatagc catctgttaa tttttgatac aaaaaaaccc
     8341 acaactgtgg gctatttgct taggtacatg tatttattta ttaatatatc tattttctta
     8401 cttgattcta ctaactcttc ttgaaatatt tctggatatt ctatgtatgt atttatctct
     8461 tccctaactg cttcaatctc attttttaac gcttctaatt cattgttcgc cataataatc
     8521 accactatta caaatttgta tatttttata tacattttat caaacaatat tatattttgt
     8581 caatattatg tcaataaaac ttactataaa aatgctttaa tttaggtatt gtatacctgg
     8641 atactaagcc tacaaacata ccagttacta atgatgccat taacataaat ggtaagtacc
     8701 ctatagcaag tatattttca atgattaaac ttgcaactat taattgacct atattatgcc
     8761 ctattgcacc tataatgctt atccctatta gtgataaatc ttttatttta ctcgctaaat
     8821 acataaataa aagacttaga taagctcctc ctatactaaa tagtagatat gataaagggc
     8881 ctgcaaatat tgatgacaaa attaccctga tagtaacaat tataacagta tctttgacac
     8941 ctaacaacat taatgaaatt aaagttataa tattgctcaa acccaatttt gcccctggaa
     9001 atataactat aagaggattt ggtatatatg tctctaatac atataaagct aaactatacc
     9061 ctatcataag acctaaaaat gtcatttttc tagttttcat taagttaact cctccaaatt
     9121 ttatctatca tcttacatta taatacaaaa tatataaaaa cacctaattt agactaaaat
     9181 atttttaatc tatttagaat aaatattagt tatttcaatg tgcaattacc tctataaatc
     9241 ttgacttttt gtatcatact tgttacaatt ttaatgataa tgaatttcat taggaggtgt
     9301 taataatgac tgtttacgat ttaaaaatag gagaaaaagg atttattaat agtatagatg
     9361 gagatattaa attatctaaa agattgcttg cacttggatg tataaatagt actgaaatag
     9421 aagttaaaaa ggttgcccca cttggagacc caattataat tagatttaga ggctttgacc
     9481 ttgcgattag aaaatctgat gctaaaaata tttctttaca ataatagagg aggagctacc
     9541 cgtgattaat gtagcattat taggaaaccc aaatgtcggt aaaactactg tttttaatct
     9601 acttacaggt tcaaatcaat atgtaggtaa ctggcctggt gttactatag aaaaaaaaga
     9661 aggatttctt ggaaaagaga taaaggttgt cgatttacct ggtatatatg caatggatac
     9721 tttctcaaat gaagaaaagg tttctaaatc atatttagaa aatgaagatg ttgatgtaat
     9781 agtaaatgta gttgatgctt ctaatttatc aagaaacctt tacttaacaa ctcaacttat
     9841 gcaatttaac aagcctatag ttatattgct gaatatgtta gacattgcag aatctaaagg
     9901 agttaatata gatgccaaaa aattaagtga agaacttgga gtaattgttg ttccaattat
     9961 agcaaaaaag aaaaatggtg ttgataggat agaagaagag attaagggag ctacgcaaaa
    10021 agcttttata tatcagaaaa actttggaag tgaaacagaa acatataaaa aattagaatc
    10081 cattttatca agatgtacaa aaactacata tacacaaaag aaatctatca gtgataagat
    10141 agacaatata gtcttgaatc ctatattagc atatccaata tttattggtg tattgttcct
    10201 cttatttaaa tttacctttg actgggtcgg aggtccacta caagagggat ttgctagtct
    10261 tatagaggct tatatttctg cacctgttag tgatatgtta tctaactcta gcccatggtt
    10321 taaatcactt atagttgatg gaataattgg aggtgttggt ggtactttac cattcttccc
    10381 tttaatattt acattattct ttggtatatc gttgtttgaa gatagtggat atatgtcaag
    10441 aacagcattt ttaatggata aagttatgag aaaggttggt ctatctggta aagcttttat
    10501 acctatagtt atgggtatgg gatgttcttc tccagctata atggcaacta gaacattgga
    10561 aagcgaaaaa gatagaagag ttacagctct tattgcacca cttatgactt gtggagcaaa
    10621 gctacctata tatgcactat ttgtagctat attctttcca cataatgctg ctttagtaac
    10681 tacatcactg tatttggtgg gtatagttat ggcaattgta gttgcattat tcttaaataa
    10741 aacagccttt aaaacagagg ctgaaccatt tatattagaa ttaccagaat atagaatccc
    10801 tactatagat gcacttatta aaaatacttg gaataaatca aaaggtttct tgataagagt
    10861 tgttactgtc atgtttgcaa tgtcagtcgt aatttgggga ctttcttcat tcaatgtttt
    10921 tggatataca gaagatataa atgtaagctt cttggctact ctaggtcata ttatatctcc
    10981 tatattcaaa cctcttggtt ttgatgattg gagaacttca gttgctattt taagtggtct
    11041 tggagcaaaa gaaatcgtag taaactcttt aaatatacta tatggtaatc taactgttgt
    11101 cttaccaaca atatttaatg gagttactgc atatacattc ttgatattta ctgctttata
    11161 tactccatgt atagctgcac ttgcaactat gaaaaaagaa tatggcaata aaatgatgtt
    11221 tacatcattt gcatatcaat ttatactagc atggataatg gcttttatag ttaaaagtat
    11281 aggtggagta ctatttatgg gtaattcaat aatagaagct ttgataggtg gaataattat
    11341 agtccttgca ctctttattc tgtttaataa attcaagtct acaaaaaacg gaaatgtctg
    11401 tagtggatgt tctatttgtt ctggatgtcc aagtgctaat tcttgctcaa gtaaggaaga
    11461 cagcaataaa aaaaataatg cacactaata tataaatatt ttagtttaaa ataagataaa
    11521 agtgggtgtc tcaaaattaa ctttttagtt catgaggcac tcttttttat agaaaatcaa
    11581 atatattttc atcatttcaa cagtaaaaaa gagcttatct agtaaaaaag aggcttatct
    11641 ctattttgag acaacctctt tttttgttac ttattcagtt tcttgctacc taacttcatg
    11701 tggaacctag tatcttttgg aacacttggg tctacacctc ttaatgtagg tatttcccca
    11761 catattagtt gtataggtac tataaaatta aatgtttgat agtaaggatt atctgtgata
    11821 tcaatcttta aatttttatc attttctgta acatctctac ctatagcaaa tacattttcc
    11881 gtccaaccag aaagtacatc aatcatctta gttactctag gttcttttcc tgtatctagt
    11941 ataaatatag tagaatcact atttatagca ttatatatac catgtataaa ctcttcaaac
    12001 tcatacccag ttacaggtat tctcattgtt tccaataatt ttaatgctgc ttctaatgta
    12061 tctccatata tatcagagtg acctattatt cttatttctt tagaattaac taacttttct
    12121 ttgtttcttt caatccattg tttagagagc ttatacactg cttcaaatct attaattgcg
    12181 tctaatatct tatttatttc ctcattgtac ttttcactag atattatacc tttttctcta
    12241 gctatttgaa gacctaataa cattaagttt aactttgtac aataatatcc ttttgtttta
    12301 gctccagatt tttcttcacc acaatttact gttaatatat aatcagaaat ctcatctatt
    12361 aaagcattct tacaacctgc catagatgct attttacagc ccttatcttc agctaacttc
    12421 atagcgttgt atgtagaata actactccca ccttgagata cacctactac tagggtattt
    12481 tcattatcaa acttgaatgt atcttcagtt atcataaatg gatacatttt cacaacatct
    12541 atatctaata aattttgtaa atatggttgc acttgcactc ctgagtgata gctagtacct
    12601 gaacctgtta ttataatctt ttttagattt gttctcttaa cttcattaaa taagctatct
    12661 gcattagata taatttctct cattctaact ggtgtttcta acatataatc ttgtattgtc
    12721 attttataca ccttccttat ctatatattc aataaattat aattaatttc tataacaaac
    12781 ctatagcatg taaagcgatt cctgttacta tagtgcctat agttaatttt gttacactta
    12841 cctttctctt taacatgtag tatattacaa atgcaaaggt taaaggtaac atattaggta
    12901 ttattttatc tagtatatct tgtaaaatta cttctgcacc atttaagttg aatttcagag
    12961 gagtagttat actcaacata gttgcaacca ttcctcctac aacgaataaa ccaacagttg
    13021 tagccataga cattattttc tccataagtc cctctctttg tattctttca agagaattta
    13081 cccctacttg ataaccatac ttaagtccaa atattctaag ggcaaatgct ggtatattgt
    13141 ataatagtaa gaataatagt ggtccaaaga tattaccttc ttttgcaagt gatactccta
    13201 ctcctgctgc tataatccta aatgttcccc agaataatga atcaccaatt cctgcaagag
    13261 gtcccataag agctgtttta acagcactaa ttgaactttc atcaaattct ggattattag
    13321 cattctcttc ttccatagct gctgttatac ctaatatagc aggaactact actgtcgttg
    13381 tattaaaaat ctccaaatgc ctttctattg cttttgcttg attttctttc ttattgtata
    13441 gcttctttat tgcaggtaac attgaataac agtaacctaa gttttgcatt ctttcgtaat
    13501 taaatgcacc ttgaagaaca aatgaacgcc aaaacatact tcttaattct ttttttgtta
    13561 atttattcaa agtcaatctc tccttccgtt aaagtattga ttgctaattg aggattttga
    13621 ttattttctt tgttattgat ataaaagttt agtacaaatg ctacacatac tccaaataaa
    13681 gctatagcag ttatatctaa tccaccataa gatgctaaca aaaaccctat aaagaaaaat
    13741 acagacatct tttttgaaag taacatatcc ataagcaatg caaaacctac tgctggcaac
    13801 aatgttcctg caacacccag tccgtctaat ataactttag gtattgcatt tatcaatgct
    13861 gtaactttat cagcaccaat cataattgct aaaaaggttg gaactgatgt acttaaaaag
    13921 aataatatta ctggcaacca catcattatg gtaactttct taaaatcagc ttgtttagca
    13981 tagaaaactg ccttttgaga aaagtacgaa tttattattc taactaatac tccaagagat
    14041 tgtgctaaga ctgctactgg tactgctact gctaaagcaa cttctgcacc attacctgat
    14101 aatatagcaa aagctgtacc taatacgcct cctgttacta catctggagg agtagctgca
    14161 cctattcctg caattcccat ccaaattaat tcaagctgtg caccaatcat aattcctgat
    14221 tttatgtctc ctaaaactaa tcctactaac atacctgtaa ctattggcct atcaagcata
    14281 gattgaccaa atatacgccc atctagtata ccaactcctg ctataatagc taacatagta
    14341 gcttgtacta acatattgaa cctccatttt aacttagttt aatttacatt aaattacatt
    14401 acatttttga ttttgttttg attttctaat aactcattta cataaacaat tccatttttt
    14461 gcttcctcta atcctgattt aactattagt tttgtatcct cctcaactaa taaaagtata
    14521 gataaaagca ttggtaaatt tactcctgtc aataattcaa atttgaaccc ttgtaaaagt
    14581 aagtttgtac atatgtttgc cacacttcca ccaaaaacat ctgttaagac aacaaattct
    14641 tcactatctt tattatttga taatatactt ctaatttctt tctcagcatc atctgctgat
    14701 ttatccgttg tcatacaaaa atactcaata ttagttaatg aaccaattat taatttactt
    14761 gtgtcaacaa gctctctgga caattctcca tgtgttgcaa ttagaaattt tttcattctc
    14821 tttctcctaa attattttat taaatctgat agttgtttct ttttgtctct aggtaacatt
    14881 ctaaattcta tatcaatccc taaatcacct atttgtttaa agttatttat atcatcatca
    14941 tcaacagcaa ctaaatctgt aagtttcttc ttcccttcct catatcttaa tcctcctaca
    15001 tttatttctt ttaatactcc tcctgaattt ttagctacaa ctaaagcatc tttggtattt
    15061 tttactaaca ctaatacatt gcttttcttt gaagaagcaa atttttctac tatttctcca
    15121 gattcctcta ccccccttat atataaagtt acattgctag gtttcgctaa gttcagtgac
    15181 atagctttca tttggtcatt ttttgcttca tcatttgcaa ctagtattac atttatacct
    15241 agtgatgttg cccatccata tgcaacctga ccatgtatca atctgtcatc aattctcatc
    15301 aaagatacca ttttccccaa atcctccatc atacattaaa atttttattg tttatactct
    15361 catttatcta taaaagcaaa aagtatgcca aatactggca tactttttta aatttcattc
    15421 acttattttg taatcttgta ttttactgtg gtagaccata gataatatta taaatatatc
    15481 ctatctctga aattggtatt tgcacactat aactcttttc tagcacacta aatgcatttt
    15541 ttattagttt tacttcacta tgatggtttt tgattaacaa atccatatca gtatactctt
    15601 caatttcagt ttgtctaact aatctttcaa tcatacaact taagtgaaca tataaagaaa
    15661 tctttttatc atttgaaagt tttttctttt gaatattttc tatagcatta attccttctt
    15721 ctattctctc tattatcttt tttgaatcta aaatagttag actatctatt actctaatta
    15781 atgaaaaatt ctttacaata gaatcattta tgttctttat tttttcattt tctactactc
    15841 tgctaaatat tctaaaaaca tcatcttctc cttttcctga tattatatcc tctaataata
    15901 taaaatctac cccattcaca tttggattgg cagttcctac tatagccatc acatcataca
    15961 tttcaaaaac tggttcttta tctcccatag aaacaagact atcataatca taaggtatta
    16021 ttttgacatc aactattcca attaaacttt cggaaagtag atgttggatt tttatagcag
    16081 tacctatccc tgtaaaacaa gatgatatta tagcatattc tttatttttt tgaggataat
    16141 acaattgtat attaggttta gtttcttcat ccataatttc tgcaatctct tcaatgtttt
    16201 taccttgcat taataaattt cctacttcta gtgcagaaat agtggatatt tggtctataa
    16261 ataacattgg gctatttatc ttattcttta aatcttcaaa tataagatta agagagccca
    16321 tatcaaataa cacaattaaa ccattcttta tcctatctcc ctctaggtat ctagacattt
    16381 gttttgatac atcagcaata gtagcatcta taggcatatc aatagctgta aatatatttt
    16441 tacccaacat ccgattacat acattcgcca tactactggc agtagcatat ccatgtgcta
    16501 taatagtcgc attaacttga ttaaatttca ttcttataat tgttgattta ataaatataa
    16561 tcattatgat tgtatctaca ttattaagat acatatccaa tctattcgtt attaatgttg
    16621 atattttact tattatagag tattctttgt aactctcttt ttttaaatat attagtaatt
    16681 tattttctaa agctttatac tcatcagaca attgataaga ttcgtccttc aaataaagat
    16741 aagacgaaaa tgccaataca ttattcccat catattttat attgttatta acttctaaat
    16801 ttctaaatat atcctgcatt atcgatatta taagctccat aagtacgtta ttattttcat
    16861 cattatttga aaagactaaa ttgtccatta gtctaatcat tagattagaa ctctttttac
    16921 taaatttttc ctttgttatt tcattttttt ctagtctatc aaagtaatct aaacatttta
    16981 tgaaactttg atagattata tttcttttat ttcttccatt tgacatttca atactaaatt
    17041 gtttaggaga aaagataatg cttttgcttt taccactttg gaattgaaat atatctacat
    17101 tttctatata aagatttcta ggaaggtctt gaagagtaac aaaaatattt ccatttcttc
    17161 tttgattttt tgcataagct cgtccacatg catattttat tacattttta cattcaccta
    17221 tattcccctt gaattttatc tttaaaatcg catttaatgt aatttgagtt atctctatag
    17281 gcaaaccaag taccttactt tcttcataaa taaaatgttc tacaatcttt cttttttcaa
    17341 tagtccctct ttcttctata ttgggaacat aaatatttat gggtattctt cttaaaaagg
    17401 tttgtaaaaa ttcagataga ctttctgttg tagcaaatat taatcttacc ctagcttttc
    17461 taattactcc attctctcca atcctagaaa acgtacctgt atccataaat gtaaataact
    17521 tttcctgacc ttcactattt aatctatgta cttcatctaa aaataaaaca ccaccatctg
    17581 attgttctat tagacctgct ctagattcat ttgctccagt atatgctcct tttacatgcc
    17641 caaataatag actagacaat aactccaaat tattataata ctgcgcacag tttaacacat
    17701 tgtatggaga atttttttct aatacattac tgcttacact atatttgtat attatcttgg
    17761 ctaaaaaagt ttttcctact ccactttcac ctgtcagcat tattgtaagt ccgccatttg
    17821 gataaagtac agcggttttt atttgttcga ttacttcttt taagctattc tcatatccta
    17881 tgatactatt aaacacagtc tcttgcttta ataatttaca ctcattttta agttcatcta
    17941 aattttcata aatacttttt tttgtaggtc ctactacatc atttatttta tttttaggta
    18001 aaaaaataac aggtctactg tttatcttta taagattatc agattttact aaatcattta
    18061 ataatctact tgattgagtt ctacttaaat taaacttttt tactatatct tctgttatta
    18121 tgccactttc ttttttaagt tcatcaaagt tgttaaataa tacacattca tttacaaaac
    18181 ttaaaatttc atccctcatg ttcaacctcc aatacaaata tcaataatac tataatatta
    18241 gtatatcatt tatatattta tgaaaaagat aat
//
LOCUS       DAELNY010000067        18159 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.067, whole genome shotgun sequence.
ACCESSION   DAELNY010000067 DAELNY010000000
VERSION     DAELNY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.067"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..817
                     /locus_tag="KPE55_001955"
     CDS             <1..817
                     /locus_tag="KPE55_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG0493023.1"
                     /translation="KYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSREYYETL
                     KTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYLPEYEV
                     RMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLAKEVIP
                     KVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTNYEEHT
                     YEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT"
     gene            807..4274
                     /gene="addB"
                     /locus_tag="KPE55_001956"
     CDS             807..4274
                     /gene="addB"
                     /locus_tag="KPE55_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG0493024.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            4274..8101
                     /gene="addA"
                     /locus_tag="KPE55_001957"
     CDS             4274..8101
                     /gene="addA"
                     /locus_tag="KPE55_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG0493025.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEN
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            8147..9358
                     /locus_tag="KPE55_001958"
     CDS             8147..9358
                     /locus_tag="KPE55_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG0493026.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            9345..12872
                     /locus_tag="KPE55_001959"
     CDS             9345..12872
                     /locus_tag="KPE55_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0493027.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNKIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKLDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKIKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            13003..14256
                     /locus_tag="KPE55_001960"
     CDS             13003..14256
                     /locus_tag="KPE55_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0493028.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            14405..15457
                     /gene="ytvI"
                     /locus_tag="KPE55_001961"
     CDS             14405..15457
                     /gene="ytvI"
                     /locus_tag="KPE55_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0493029.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            15548..16774
                     /gene="pepT"
                     /locus_tag="KPE55_001962"
     CDS             15548..16774
                     /gene="pepT"
                     /locus_tag="KPE55_001962"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG0493030.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            17000..18052
                     /locus_tag="KPE55_001963"
     CDS             17000..18052
                     /locus_tag="KPE55_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG0493031.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         7722 a         1721 c         3301 g         5415 t
ORIGIN      
        1 aaaatatttt tcttatagtc aaaattcaat taacacatat aaatcatgtc cattgaaatt
       61 taaatataaa tatattgata gaataaattg gaaaaatgat gatgttggta gtagagagta
      121 ttatgaaact ttaaaaacag gaagagattt tcatttgatt tgtgaaagat attttagcaa
      181 tatacctctt ggaatctatt ttaatgagga tgataaaaat agtaaaaagt ttttaaagtg
      241 gatggaaaat ataaaaaaag ttgttcctat tgaaaaagga aaaacgtatt tgccagaata
      301 tgaagtaagg atgacattaa atggggatat aatacaggca aagtatgatt tagttgtaat
      361 tggaaataac aatatagaaa tatgggattg gaaaaccgag tctaaaaaga ttgactataa
      421 acacgtagaa aatagaatac aaactattgt ttatatgttt ttagcaaaag aagtaatacc
      481 taaagtttta aaaatggaca taaatgttaa agatataaat atgagatatt atcaacctga
      541 atttgatgac ttaccaatta ccattttata tgatgaagaa aaacatgaag caaatagaaa
      601 taaaattcaa aattatataa gtatgataaa aaacactaat tatgaagaac atacatacga
      661 agacaatcta tacaatgata tagaaaaggt atataataaa gaagaacgta tgtgctatag
      721 aaataaaaag cattgcaagt attgtgaatt taataagtta tgtaatggca aagaaataga
      781 ttatagtatt ttggaggcgg aaatttatgg gacttaggtt tgtattaggt agaagtggaa
      841 gtggaaaatc cacctatata ctagatgaga ttaaaaaaga agctcaaaaa aatgaaacga
      901 catcaattat acttttagtt ccagaacagt acacatttga ggctgaaaat agagtaagta
      961 aactattttt aggaaaagaa aaagataagt atctaagagt tagggtactt agctttaaga
     1021 cactaagtaa tatagttttt tctcaagtag gaggattgac agatgtaaat ataaattcta
     1081 gtggaaaagc aatgatggta tatagagcta ttgaagatgt aagtgaagaa ttaaatgtgt
     1141 tttcaaaatc aaaatcgcag tctggttttg taagttcaat tacagatatg atatcagaaa
     1201 tgaaacaata caatataagc cctgaaatgt tagaaaatat atctggagaa ttggataatg
     1261 aaactttaag tttaaaatta aaagatataa gtaaaatata taattctttt gaagggaaat
     1321 tgcatgaaaa ttatgtagat gctcaagata tgttaacttc attggcaagt aaaattgaat
     1381 taagcagtta tttagatgga gcttgtgttt atatagatga atttacaggt tttacaccaa
     1441 atcagtacaa tgtcataaag agtatattaa ataagtctaa aagtgttaat atctctttga
     1501 ctgtagatga tatcaactat attgggtaca gtaaatctga tatgttttct agaacgaaat
     1561 ttacttattc aaaattgact caattgtgca acgaagaagg cataaaaata ttgccacaag
     1621 taaatttaaa cacaggtgtt ataaaaagat ttgaaaaagt taaggaatta cagcacttag
     1681 aaagatttta taatgcttat ccatataaaa tatattcaaa tccaactgaa aatataaaga
     1741 taaaagagtt taacaattta tattcagagg tggaagagat agccagagaa attgtacatt
     1801 tagttagaga taagaatgtc agatatagag atataaccat tgctacaaga gatttaaata
     1861 gatatgattt tttagtacac tctatattta atgaatataa tataccaaac tttatagata
     1921 agaagagaga ggcaaaaagt aatccaatag ttattttaat tatttcagct ttagaaatga
     1981 agaatagacg atatgggtat gaaactatgt ttaggtattt aaaaagtgga cttataggaa
     2041 ttgataatga tgatataaat ttactagaaa attatgtgtt ggctaatggg attaaaggta
     2101 aaaaatggtt tgatgaaaaa tgggattata gaataactca aagtctttca ggtcaagaaa
     2161 gtgaatttga actggaatta aaagagaaaa taaatgaaat aaaaaataga gtattagagc
     2221 ctatagtaat tttacaagaa aaattaagag gaaaaaatag agtaaaagaa atctgtagat
     2281 atatatatga atttttatta gatataaata tgccagaaac tatagaaagt ttaatagtaa
     2341 attttaagga taaaggggaa cttgatgttg caaatcaata ctcacaagtt tgggacatag
     2401 tagtggacat attagaccaa atggtggaac ttatgggtga tgaaataatt tctttagaaa
     2461 aatttataaa attaataacc ttaggttttg atgaatatga attaggactg gttcctccta
     2521 gtatagacca ggtacttgta agtagtgtgg atagaatgaa aaatccagac acgaaatatt
     2581 tatacctagt tggaactact gatggtgtgt ttccgctgat tacaaaggat agtggaatat
     2641 tgagtgataa tgatagggaa agtcttggaa acaaaggaat cgaagttgat attgatagta
     2701 agactaggag ttttgaagaa cagtttcttg tatataaagc tcttacttcg acatctaaga
     2761 atttaaccat aacatatcca atatcagacc atgaaggtaa aacgcttaga ccatcaataa
     2821 ttatatcaag acttaagaag atatttccaa atatagaaaa taaaagttat ttagtggaag
     2881 aaaataaaag cactgataaa gatatactta aaaaaataac ggttaaatcc cctacattta
     2941 atgaacttat aaacataata aaaaattatg acagtgatgg ttacaataca gaagagatta
     3001 atagtatttg gttagatata tataggtact atttaaaaga tgaagtctat agttctataa
     3061 caaaaaaagt tataaaaggt cttagttata ctaatcaagt tcataagatt gaagagaaaa
     3121 aaataaggtc attgtataaa agtaattctc ttagcatatc taggcttgaa aaatatgctg
     3181 aatgtccatt tgcttatttt atacaatatg gtctaaaagc aaaaaagcgt aaagagtatt
     3241 catttacacc accagattta ggaactttta ttcataatat tttagatagg ttttcaaaag
     3301 aacttttaca agataattta acttggagag atattgatga aaaatatata gaattaaaga
     3361 taggtataat agttgatgaa ataattttaa agataccagg atatatatta aatagttctg
     3421 agaggtataa ataccttgca tatagattaa agaatatgtt gacaactgct ataactataa
     3481 ttagtcagca aataaaacaa ggttcatttg aaccaataga ttatgaagtt aaatttggca
     3541 acaatggaaa atatccacct ataaaaatgg tgttagaaaa tggacaagaa gtaagtttaa
     3601 ttggacaaat agatagagtt gatgaatttg aagaaggaga aaataaatat ataagaataa
     3661 tagattataa gtcaggcaat aagtccataa gtctaacaga aatatattat ggtctacaat
     3721 tacaattact tgtgtattta gatgcaatcc ttgaaagtgc aaaggatgaa gatataaata
     3781 taaatccagc tgcaatacta tattgtagaa taaataatcc aatagcaaaa tttaatgaag
     3841 ataaagatga tgaagaaata caagaagcaa tattaaaaga acttagaatg aaggggcttg
     3901 tagtaaaaga ctctcacata gttaaagaga tggataaatc attaatagat ggagaaagga
     3961 aaaactcttt agtaatacca gtaggtctta ctaaggatgg aaatgtaggt aaaagtactt
     4021 cagcaataag ctatgaggat ttcaaactgc ttagaaaata tgtaaggcat gctataaagg
     4081 atttatgtga agaaatgtta agtggagaaa tacgtatagc tccatataaa cataaagatg
     4141 gaacatcatg tgacttttgt gattactctg ctatatgtca gtttgattca actataaaag
     4201 ataacaagta taaaaattta aataataaga gtaatgaaga gataataaaa atgatgaaag
     4261 gagatgttaa ttagtgagtt ctcctaaatg gacaaaagag caattagaag ttatagaaag
     4321 tagagaatgc aatttattgg ttgctgctgc tgctggttca ggaaaaacag ccgttctagt
     4381 tgagcgtata attcaaatga taacaagtag agaaaatcca attgatatag ataagttatt
     4441 agttgttaca tttactaatg ctgctgcgtc agaaatgaga gagaggatag gggatgctat
     4501 aggaaaagcc ttagatgaaa acccagaaaa taaacattta caaaaccaac ttgttttatt
     4561 aaataaatct agtattacaa caatacactc attctgtttg gatgttataa aatcaaactt
     4621 tcatagaata aacttagacc ctaatttcag aataggagac caaactgaat gtgctatttt
     4681 gaaacaagaa gcaatagagg aagtatttga agatttatat gaagaaagag atgaggggtt
     4741 tttaaattta gttgaaagtt atgcagaaag aggaggagat aaagaagtac aagatattat
     4801 cttgggaata tattcatttg caatggcttc tcctgaacct aaaaaatggt tgattgactc
     4861 agcagaacga tttaatattg atgaaaattt tgatttttca cagtctattt gggctagagc
     4921 tatattagac actgtaaaaa tagagattaa tggtctttgt ctaaatatgg aaagggcatt
     4981 aaaagaagtg gaatcaatag aagaattaga aacttttgca gaaaagttat ctgttgaata
     5041 taaaaaaatt gctgacattt cacaagcatg taataaatct tgggatgagg catataaaaa
     5101 gatggcaagt atgtcctttg aaaattatgt aaaaggagta aaaagaattt caaaagatgc
     5161 tccttcgtat ataaaagaat caaaagaaaa ggcaaaaact ataagagata agactaagaa
     5221 gtctttagaa agtatagtaa gtgctacatt caataaagat aatgattcta ttagagaaga
     5281 aataaaatat ctttataaca tagttaaacc aatatctagt gttgtactga gatttgaaga
     5341 ggaatatagc aataagaaaa gagaaaaagg aataatagat tttaacgata tagagcattt
     5401 tgcattaaat atacttacag atgtagatga aaaaggtaat attgtacctt ctgatatagc
     5461 agttggatat agaaacaagt tttatgaaat atttatagat gaatatcagg atagtaatct
     5521 agtgcaggaa gtactgctta aagctgttgc aaatacagaa actccaaata gatttatggt
     5581 tggtgatgta aaacagagta tatatagatt tagacaagca aagccagaat tatttttgca
     5641 aaaatataat aactataatg ataaaaaggg aagttcccat agaaaaataa tgctttacaa
     5701 aaattttaga agtagagaag aagttgtaga tgctgtaaat tatatatttg aaaatataat
     5761 gaatgagaat ataggagaaa ttgaatatac agaaaaagaa agactaaatt taggtgcaaa
     5821 ttttaatgtg gatacagacg agaaatctat tattggtgga gcaacggaga tacacctaat
     5881 acaaaaagat aataaacttg atgatgatat tataaatgat aaagacgata gaattaataa
     5941 caaagaaaat gaaatagagg aagaagaaaa tttagataat atacaacttg aggctagaat
     6001 ggttggaaac attatcaaag atttaatgaa agtcaatgaa gatggaaaaa ttcaaaaggt
     6061 ctatgataag ggaatagatg gatatagacc tgtggaattt agagatatag ttattctttt
     6121 aagagctaca tctgcatggg ctccagtgtt tgcagatgaa ttgatgaata tggatatacc
     6181 aacgtatgct gatgttggag tggggtattt tgatacgatt gaaataaaaa caatattgtc
     6241 attacttcaa ataatagata atccaatgca agatatacct cttatatctg tattaaaatc
     6301 acctatattt ggatttacac cagaggattt gattgatatt agagttcaga gtaaagataa
     6361 aattttttat gaggttttaa aaagtacagc agagtatgat ggatttacag attctcaaaa
     6421 tgaaaatgag tctgagttta taccaagtga agagtgcata aacaaaagta aagatttttt
     6481 aattaagcta aaggaattta aagaaaagtc aatgtacatg agtacagatg aatttatatg
     6541 gtacttgtac acaaggactg gatactatgc ctatgttgga gcattgccag gtggaagtca
     6601 aagacaagct aatttaaaag tattgtttga gagagcaaaa caatttgaag aaaccagtct
     6661 taaaggaata ttcaactttg taaattttat agagaaatta aaaaaatcta gttctgatat
     6721 gggaagtgca aaaacacttg gtgagaatgc aaatgttgtt agaataatga gtattcataa
     6781 aagtaaaggt ctagaatttc cagtagtcat atgctctgca atgggtaaaa atttcaatac
     6841 tcaagatttt aaaaagagta ttttatatca tcataattta gggtatggac ctcaatttgt
     6901 agactatgag agaaggattt cttttccaag tatagccaaa gaagcattaa aaagcaaaat
     6961 aaatatagaa aatttatcag aagagatgag agttttatat gttgcattta caagagctaa
     7021 agaaaaattg attataactg gttctactag aaatatacaa gatagtataa agagatggtc
     7081 aaatggaatt gaaagtttag atacaatatc acaatacgag atattaaaag gtaaaaattt
     7141 tttagattgg ataatgcctt gtgtattaag acatagggat ttatctaatc tactagaaga
     7201 agtaggatta gacgcagttt ttaatgttga acataattca aaatggtatg gaaagttgtg
     7261 gaataagaat gatattttag tggaaaagaa gagtgatgaa gaaaaagaaa gtattgaaga
     7321 aattcttgaa aaaatagatg tagataatcc tgacagtgat tattacagtg aaatagaaga
     7381 aaaattaaac tatatatatc catatgaatt tagtacaaga aaacctgcta ctatatctgt
     7441 tacagaaata aaaaagatac aaaataatta tgaagaagag ttaataaata ctatatttga
     7501 acaaaaagta atattaaaaa agcctctatt tattcaaaat gaagaagaaa gagaaaaaat
     7561 aagtggaaca gaaagaggta caatagtcca tttagttatg gaggttttag acttaaaaaa
     7621 tgtaagtagt gtaaatgata taaaatcaca aataagaggt tttgtttcaa aaggtataat
     7681 aacagaaaaa caagctagta tagtaaatcc atataaaata tataaatttt ttgcctctaa
     7741 cataggaaaa agaatgttga atgctgaaat tataaataga gaaaaatcaa tttatgcaca
     7801 agtaaatatg aaagacatat atatttatga aaagcttata aacaatgacg ataaaaaact
     7861 ttatgataat gaaagtgtca tgttaagagg tatagttgat gcttattttg aagaagataa
     7921 tcaaatagtt ttggtggatt ataaaacaga ttttgtaaat gaggaaaata taaatcaaat
     7981 aatagagaaa tataaaaaac agttggattt atatgcagat attattgaaa ctttaactgg
     8041 taagtcagta aaagagaaat gtatatattt atttggagtt gatgaagctg tttgttatta
     8101 gtttttatat ataaattaaa cctaaaagat agaggaaggg gaaaaaatga gatttattca
     8161 tacatcagat tggcatctag ggaagtccct agaaggccat tctagaattg aggaacaagc
     8221 aaagttttgt gaggaattta taaaaatagt agaatcaaat gaaatagata tggttataat
     8281 tgctggagat gtatatgata cttctaatcc accagcacaa gcagaaaaac tattttatca
     8341 gactgtatca agacttgcca ataatggtca gaggtgtgta ttaataattt caggaaatca
     8401 tgataatcca gagagactct ctgcaataac acctctagca catgaacagg ggatacttat
     8461 atatggttat ccattaagtg ctacgataga agctaagtat aaagggtttg aaataacata
     8521 tgcaactcag ggatgtacaa aactgaatat aaatggagaa aatattgtgg ttgctacatt
     8581 accttatcca agtgaaaaaa gattaaatga agttttttca agtgaagatg agtttgagaa
     8641 acaaaaaaat tattctgaaa aggtaggaga tatatttaga agtttagaag aaaattttag
     8701 aagtgatact ataaatatag ccgtatcaca tatatttgta ataggtggag agagtactga
     8761 atcagaaaga cctatacagt taggtggaag ttttttagtt gaaagaaaag atttgccaga
     8821 aaaggctcaa tatactgcac taggtcattt acataaacaa caaaaagctt ccgaacgttt
     8881 aaatgcatac tattctggtt ctccacttca atatagcaag gatgaaagag cgtataccaa
     8941 aggggcatac atagtggata taaaagctgg tgaaaaacca ataattgaag atgtatattt
     9001 taataattac aaacctatag aagtatttaa atgtaatgga atagaagaag cactagatat
     9061 atgtgagcaa aatcaagata gagagatttg gtcatatttt gaaataaata ctgatgagat
     9121 aatatcacag aatgaaatta aaaaaatgaa ggaactatta aaagacataa tagaaataaa
     9181 accaataata acatcttgtt atgaacaaga aagtgtagat ataaaagaaa agtctatggc
     9241 agagttgttt agagagttct attcttttag taaaggtgta gagccaaaag gagaacttat
     9301 ggatttattt ctagatataa taagtgaaga aggtgaatca gcagatgaga ccaattagat
     9361 tagaacttac agggcttaat agctatattg ataaacaagt tatagacttt gaaaaattaa
     9421 ttgaaagagg tttgtttggg atatttggaa ctactggaag tggaaaatct acaatcttag
     9481 atgcaataac tattgctatg tatggaaata tatccagaaa tacaaaagaa tatataaaca
     9541 gtgtgtgcga taaggcaatt atatcatacg agtttgagat aggaagcaaa aacactagaa
     9601 gaagatacat tgtagataga accattgcta gaagcaaaac aggaacaaag acctctaatg
     9661 ctagattaat agaagtattg aatgacaata cccaaaatgt tttagctgat aaagttgtgg
     9721 aagtaaatga aaaagtagca caggttgtag gattaacggc aaatgatttt actaggtcag
     9781 tagttttacc acaagacaaa tttaatgagt ttctaagatt atcacgtgca gatagaagag
     9841 acatgttaga gagaattttt aatcttgaga aatatggaag aagtcttgga gaaaaagtga
     9901 aaaagagaaa aaatatacag ttacaaaatt taaaagattt gaaatcaaaa ttaagtcaat
     9961 atgatggaat tacagaggaa gtatacaaca aaataaacca agaattaata gagttaaaaa
    10021 atttggaaaa agataagaat aatgctttag acctagctca aaaatcttat gaagaaagta
    10081 aaacagtata tgaagaacaa ttaaagttag aaaaaaatga acttagaaaa aatgagcttg
    10141 atttaaaaaa tagtgagata aaagaaaaga ttgctcttgt agaaaatgat agtaacgcta
    10201 aaaaggtaga cccatacata agttcagttc aaaatctaga aaaaaagcta gatgaagata
    10261 gttttgcagt aagtaattta gaaaagaaat tagcaatatt aaatcaggag cttgaagtta
    10321 caaaaaatag atatgaaaaa ataaacaaaa ttaagaatga agaagtccct aaattgagtg
    10381 aagaaaaaat aagattgcaa caagcaataa aattagaaga agagttagtt ttattagata
    10441 gagagttaaa agagctaaaa gaaagtggaa ttaatttaaa taagactaaa gttgaattag
    10501 aaaaagtaaa gcaggtgtca gagtccagaa aagatgcagt tactaaatct ataaaagaag
    10561 ttgaaggaaa gattgataaa gtaaatataa gtgcagaatt aaaacaaaaa atatttctag
    10621 catatgaata cgagaaagat tacaacaacg tattggaaga aaaaaatcaa aagctaaata
    10681 aactagaaga aatactcaaa gagacagaaa atataaattt aaaagttaga tatattgata
    10741 aagataagaa tgatgtaaat cgtaatttag aaaatttatc acttcacttg gatgttcttc
    10801 ttaaaaaatg tcctggtaag agtgctgatt tacttttaaa atctgaatat gttactgaat
    10861 taagaaataa agcaaataat actaaagaaa atgaaattaa gaaaagcagt atacaagatg
    10921 aattaaagat aatacttgaa agtaagttta atactgaaag ggaagtaaat ttactaaatg
    10981 aaaaacttga aaataataga aagaacagag atgacttaga aaaagaattg gaagaactaa
    11041 agtatttaaa tttagcctct gaacttagac gtgaattaaa agaaaatatg ccttgtccag
    11101 tttgtgggtc aaaacatcat gaaaatcatg atataactaa gtatgatgaa aatatatctt
    11161 ttgtcaaaga aaaactggag aaattagaaa aagaaaaaat aagtataaga aacaatatag
    11221 aggaattaaa tgctaaagta agtgggtatt tatctataga aaaaatgaag actaaagaac
    11281 ttgaagatgt aaaaggaaaa ctagggggga ttccatctag tcaattatta aaaaaacttg
    11341 atgaagaaca aagaaaactt gcattgttaa aatctaatat tcaggagtgg gagaaagaaa
    11401 aagagagtac tgaaaataaa gtaacattag ctaaagaaga aaaaaataaa attgaaaaag
    11461 aagaattaaa aataagagag agtctgaata attataagaa attaacaaga gatttaaaca
    11521 tagaaataga aggtttagaa aataaatgta aaaaaattaa acaggaatac ctaggattaa
    11581 agacgattac taaagtctct aatctattgt caaaagtaaa agaaataaga gagaatgaaa
    11641 aagaattaga attactaagt actgggcatt caaatttatt aaaaaataga gactcactag
    11701 atattaaaat aagagaaaat gagagtaaac tacatgaaat agagatagaa ttaataaagg
    11761 ctagagaatt atatgtagaa aagaaattga gtagggataa taagtataaa gaagttatta
    11821 gcataacgaa aggtgattta gctaaaaatc ttttacataa tgttgaagaa aatatatgta
    11881 agatattaga acaagaagaa tcaagtaaga aaaaattgga agagcaaaga ttagaatatg
    11941 aaaaaaacat tgctgaaaag cacaatatag atggtagact taaaactgct aaggaacaat
    12001 ataaagacca aaaatcaaca ctaaataaac tattagcaga aaataaattt gaaagtatat
    12061 atgcagtaaa aagagcttta ttagatactg atgcagtaaa aaaattgatt gaagaaatac
    12121 tagagcatga agaagaacaa aagttattat catttaaaat aaaatcatca aaagaaaaat
    12181 taaatggaag aagcataaag aaggagtact ttgaacagtt aaaagatgaa atttataatt
    12241 tgaaagtaga aataggaaat atatccaaag atattggagc taatcaaaat cagttaataa
    12301 cattaaaaga ctctttagat aagataaatg attttaataa acaacttaag gtagttgaac
    12361 acaatgtgga cttattggaa gaattagata aatgtgtaca aggaaataaa tttgtagaat
    12421 atgtttctac aaaccaatta aaatatatag cattggaagc atcaaaacga ttggatggaa
    12481 taacaaaagg aagatatgca cttgaaattg actcaacact aaactttgta atgagagata
    12541 actttaatgg aggagaaaga agaagcattg atactctatc tggtggagag acattcttga
    12601 catctctatc attagcactt gctctttcat ctcaaataca attaaaggga agtgcaccat
    12661 tagaattttt ctttttagat gaaggttttg gttcattaga tagtgaactt ttagaaattg
    12721 ttatagaatc cttggaaaga ttgcatagta ataatctaag tgtgggtata ataagtcatg
    12781 tcgaggaact taaaaataga gttcctgtta agctattagt ttcatctagt gaggctggaa
    12841 taggttcaaa agtaaaaatt gaatatagtt agaaaatata tgttaattga atatattatc
    12901 taatatttcc aaataatatg taattgtgat ataataaaaa ctaatattaa agttatttat
    12961 aattaatatt aatataaaaa catgtaagta ggagtgtggt aagtgttgga atcgcccaat
    13021 aatttgattc agattatttt tttaatagta ttacttatag ggtctgcatt tttctcagca
    13081 tctgaaacgg ccttaatgtc gttgagtaaa atcagaatca gatatatgca agatgaagga
    13141 gttaaaggag ctaagttagt aagttcatta atagaaaatc caaataagct tttaagttct
    13201 atattggttg gaaataacgt cgtaaatata gcagcaacat ccatatcaac atctttgttt
    13261 ataggattaa tgggagaaaa aggagtggca cttgctacag ctgttatgac agtattagta
    13321 ttaatatttg gtgagattac tccaaaaaca atagcagcaa ataattcaga aaaggtttct
    13381 ctattagtat ctaaacctat aaaagcaatt atatttatat taagaccaat agtatggata
    13441 tttaatataa ttacaaatat tatatttaaa ttatttggta taacaaataa aggagctaaa
    13501 tcattcataa ctgaagaaga gctaaaaact atggtaaatg taagtcatga agaaggcgtc
    13561 cttgaaatgg aagagagaga aatcataaat aatgtttttg aatttggaga tatgcaagct
    13621 aaaaatgcaa tggtacaaag aatagatatg gtagctatag atatggaaga tagttatgat
    13681 gagataatac aggtatttaa aactgaaaaa ttaagtagaa tgcctgtgta tgaagaaact
    13741 atagatgata tcgtaggaat acttaatata aaagatatta tatttttatc tgatgaagaa
    13801 atagagtcat ttgatattaa aaattatatg agagaacctt ttttcacgta tgagttcaaa
    13861 aagataacac aacttcttga agagatgaaa cttgaaaaaa gtcagatggc tatagttgta
    13921 gatgagtatg gtggaacatc tggactttta actatagaag atttagtaga agtcattgtt
    13981 ggagatatag aggatgaata tgatgaagaa gaggatgaaa tacaagttat aaaagaagat
    14041 gagtatattg tagatggaag tactaaaatt ggtgatgtta atgaacttat tggtgtaaac
    14101 ttggaatctg aggagtttga ttccatagga ggttttataa ttggacacct aagcagattg
    14161 ccagaagaaa atgaagtaat tgaagttgat aatataagat tttgtataga aagtatagaa
    14221 aaaaatagaa ttaaaaaaat aagaatatat acataatgta ataatgttct taaatttatt
    14281 agaagtgtat aaatataaaa gatatctttt atatttatac acttcttttt attttgtatt
    14341 caaataaaac tagtatgaaa tggacaaaat aaaaatataa attagaagaa aggagatttt
    14401 taagatgagc atactagaaa ataatttttt atacaaatta aaaaacaaca ctatcttttt
    14461 ttgtatatat acattgttat ttatttttat ttacaaagcg tttccataca tagctccatt
    14521 ttttctaggt gctataatag cacttatgat taatcctata tcacaaaagt tagaaaataa
    14581 gtttcgtata aataaaggta tctctactct tgttctaagt tttcttgcag tggctatagt
    14641 cagtacagtt actacaataa tagtaataaa ttcaatgaaa gaattaatgg gatttttaaa
    14701 taatatatct gctaatcctg aggatataag taatactatt atgtattttt taaataaaat
    14761 aaatgacttt atgaaatcat ttcaagaaat agccaatttt gatgtagaac aattagtaaa
    14821 caaattcagt ggtgaagtaa tgcaaattac aaaaaatctt cttactagta tacttggatt
    14881 ggctacttct attccatata ttataatatt tataattacc ctgtttattg ctacctactt
    14941 tatagcaaaa gaccttgata agattgaaaa tagtttttat aatatgttta ctgttgatgt
    15001 tagaaagaag gtaaaaaacg taaagaaaga ggcagggctt tcacttgttg gatatataag
    15061 ggcctataca atacttatgg ctataacatt ttttgcaatc tggggaagtt ttgctttatt
    15121 tggattgaaa tacggtctaa tagtagggtt tgtaggagct ttaatggatt taattccatt
    15181 cctaggtata ataacaatat atcttccagt aattgtttat tatttcctta taaaaaatta
    15241 ttttatagct ataagtatga ctgtgatatt ttttgttttg tctttgatta gagaaatact
    15301 tgaacctaaa ctagtatcag taaatgttgg actaaatcca ttagctaccc tagcagcgat
    15361 ttttattgga atccaagtaa aaggaataat tggagtaatt ttttgtttgg gtttggtatg
    15421 tatgcatgat atacttaaga aggtggatat tttttaatta taataattga tagatttttt
    15481 taaaaataaa attttattca caggtaaata aaaaaatatt aatcttatta agaaagaggg
    15541 tatatacgtg aaacaaaaag tagtggaaag atttttaaaa tatgtatcat ttgatacaac
    15601 atctaattct cagtgtgaga actgtccatc ttcagaaggt caaagagttt tagctaagta
    15661 tatagttgaa gagctaaaaa caatgggtgt tgatgatgtg agtttagatg aaaatagtta
    15721 tataatggct actttaaaag gaaatactga tggtgtggat actataggat tcatatcaca
    15781 tttagataca attgaagatg ttagtggaaa agatattaaa ccaagaataa tagaaaatta
    15841 tgatggaaaa gacatagttt taaatgaagc gttaaatgtt attacgtatg taaaggattc
    15901 tccagagttg gaagaattta aaggtgacga tttaatagtc actgatggaa caactctact
    15961 tgggtctgat gataaagctg gtatagctga gatagtgact gcaatagagt atctaataaa
    16021 tcatccagaa attaagcatg gagatataaa aataggattt acaccagatg aagaaattgg
    16081 tagaggtgct gatttatttg atgttgaaaa atttggagca aaatatgcat atactttaga
    16141 tggaggaata gttggagaac ttgaatgtga aaattttaat gctgcaaatg caaccattac
    16201 tatacatggt agaaatgttc atccaggttc agctaaaaat aaaatggtaa atgctataca
    16261 tatagctgct gaaatatcag aaatgttccc agcagatgaa agaccagaaa caacagaagg
    16321 atatgaagga ttttggcatt taaattctat aggtggaaat gtcgagaatg taagtatggc
    16381 ttatattatt agagaccatt gtaaagaaaa atttgaaaat agaaaatcta ttatgataga
    16441 gaatatagaa aaaataaata agaaatacga taatagagtg gaattggatt taaaagactc
    16501 ttattacaat atgaaagaaa agattgagcc agttatgttt atagttgata tagctaaaga
    16561 agctatggag gaattaggta taaaaccacg tttagttcct gtaagaggtg gtactgatgg
    16621 agcaagatta tcttttaatg gattaccttg tccaaatata tttactggtg gattaaactt
    16681 ccatgggaaa aatgagtgta taccagttag ttctatggag aaagctacta aactaattgt
    16741 aagaatagct gaaaaatatg ctgaaagagt ttagttaaaa agtatttaaa aattaagcat
    16801 ttaaagttat aatagctata tcattcttta taagtcaagc tgattataag cttgtgttgt
    16861 acttaaaatg gtatagctat ttttgtaatt atttttataa aagataatta tttttataaa
    16921 agataattat ttttagttta gggtatcaat aaaaagtatg aatatcaata ggtattaaca
    16981 tttaaaaggg ggaattgtat tgaaaattaa atctaaaaaa ataatagcat ttgcactttc
    17041 acttgggatt atgttgccaa gttgtgtata tgcagacact attagtaaaa atcaaaatgc
    17101 acaggtagat atgaacaatt atcttcaagg tagaaaatct ccaaaggagg aaaaacttat
    17161 tggtgaagat agatttgata cagcgataaa aatatctcaa agtggttgga ataatggttc
    17221 agaaagggtg tttctagtta actcaaattc attgccagat gcattggctt caactccttt
    17281 ggcaagtaaa ttggatgcgc caatactgct tacaaataaa aatagtatac catataatgt
    17341 aacagaggaa ataaaaaggc ttaatccagc agaagttatt ttaattggaa gtgaaggagc
    17401 tatttcatca agtgtaaaag aatctcttga agatatggaa gtattagtca gtagaatcgg
    17461 tggagcagat agagaagaaa catctttact tcttactaga cagctagatg atactggtga
    17521 tttaggtgtc tcaaaagtgg cagtggttaa tgggtataat ggactggcag atgcaacttc
    17581 aatatcaagc ccagctgcaa gtgataatac agctataata tatacaggta aggatagcat
    17641 aagaagtgag gcaaagaatt ttattacaca aaactcaact aagacataca tagtaggtgg
    17701 ggagtacagt ataagtaaaa aacttgaagg acagttagta aactcagaaa gactagcagg
    17761 gacagataga aaagatacta atgcgaaagt tttagaaaag ttttatggaa aatcaagtaa
    17821 ggttaataat atgtattttg caaaagatgg aagtggtaga gaagctgatt tagttgatgg
    17881 gcttgctgct ggagtattag catcaaaaac aaagtcacct gtagtacttg caagtggaag
    17941 tttaagtagt gcacagaaaa gttttataaa aaaagtaaag gctgataaat ttgtacaagt
    18001 tggtggaggt aaaaatagta aaccatatgc agaagcatta gccttacaat aatattgtgt
    18061 aaatcaaaat taatatataa taatagcaag taaaccaagt tgcacttcaa tatatataat
    18121 aaaataagta aactaagtta tatttcagta taatatata
//
LOCUS       DAELNY010000068        18099 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.068, whole genome shotgun sequence.
ACCESSION   DAELNY010000068 DAELNY010000000
VERSION     DAELNY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.068"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            189..914
                     /locus_tag="KPE55_001964"
     CDS             189..914
                     /locus_tag="KPE55_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0493032.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            1044..2969
                     /gene="mngA"
                     /locus_tag="KPE55_001965"
     CDS             1044..2969
                     /gene="mngA"
                     /locus_tag="KPE55_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG0493033.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYYEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEASFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLTGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            3113..5728
                     /gene="mngB"
                     /locus_tag="KPE55_001966"
     CDS             3113..5728
                     /gene="mngB"
                     /locus_tag="KPE55_001966"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0493034.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSTQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            6007..7161
                     /locus_tag="KPE55_001967"
     CDS             6007..7161
                     /locus_tag="KPE55_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG0493035.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYEVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(7284..9197)
                     /locus_tag="KPE55_001968"
     CDS             complement(7284..9197)
                     /locus_tag="KPE55_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0493036.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     NRCPIPFVYVNPYLNRKDYDNIQSKFTQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            9439..9756
                     /locus_tag="KPE55_001969"
     CDS             9439..9756
                     /locus_tag="KPE55_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493037.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            9764..11011
                     /locus_tag="KPE55_001970"
     CDS             9764..11011
                     /locus_tag="KPE55_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0493038.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            11040..11393
                     /locus_tag="KPE55_001971"
     CDS             11040..11393
                     /locus_tag="KPE55_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0493039.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            11415..12860
                     /locus_tag="KPE55_001972"
     CDS             11415..12860
                     /locus_tag="KPE55_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0493040.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDKNGYYPKRSGIDFYHTYKEDLKLLKALGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYENVIRTNGEELREK"
     gene            13252..14004
                     /locus_tag="KPE55_001973"
     CDS             13252..14004
                     /locus_tag="KPE55_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0493041.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            14106..15026
                     /locus_tag="KPE55_001974"
     CDS             14106..15026
                     /locus_tag="KPE55_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG0493042.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            15029..16945
                     /locus_tag="KPE55_001975"
     CDS             15029..16945
                     /locus_tag="KPE55_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG0493043.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            16965..17834
                     /gene="gatY"
                     /locus_tag="KPE55_001976"
     CDS             16965..17834
                     /gene="gatY"
                     /locus_tag="KPE55_001976"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0493044.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
BASE COUNT         7054 a         1976 c         3309 g         5760 t
ORIGIN      
        1 agagatgtat caaaataaaa acttgaatca tctctatcaa tataggaagt acacattttt
       61 tatgtataat aaaaagagta gtatgcatta ataaattaaa aatattaaat tataattata
      121 ttaacgaaaa ttttagctaa tatttttgaa ttttacttat gaataataaa atagatggag
      181 gagaaactat ggaattttat gaaagagcaa aaaaatatga gtataaattt acagatacag
      241 aagacatgat aattgaatat atgagaaaaa acaaagaaga aatagtaaaa ttatcaatac
      301 aatctttagc agataaattt tttactgtcc caaataccat agttaggcta tgtaaaaaaa
      361 tggagtatga tggtttctct caactaaaaa ataatttaaa agaagagtta aaaaatgagg
      421 ataaagagcc aaaaaatggt tttgaatcag atataaaaaa gacattagag ctaatagatt
      481 atgaaaaaat aaataaagta gcagatataa ttcaaaaatc taggagtatt atagtttatg
      541 gaataggtca aaacattcct ttatgtgaga tattagtaaa agaaattaga ggtgggcatg
      601 ataatataac ttactatagt caaagacatg aagtacttca tgatataggg aaattaggtg
      661 aacaagatat gttttttgca cttagtttaa gtggagaaac gaaagaagtt atagaagcag
      721 caaagacagc agttaataag ggttgtaagg tagtagctct aacacatata tgtgaaaatc
      781 aattgcaaca aattgcagat ataagtttat attgctattc aacaattaaa caagtaaatg
      841 ggtacaatat atcagataag gttccaatta tgatgattat gagagcaata atagagcaat
      901 actggttaag atagagattt gtgtataaat acacaaatct ttatttttta agaagaaatt
      961 gtgtgtataa tttcaaaaaa acattttcat aatttatttt atgctatatt catagcatta
     1021 aatgatttaa ggagggcgta attttggaat ttaagaaaat cacaaatgaa aaccttgttt
     1081 ttttaaaaca aaaaataaaa acaaaggaag acgcaataaa gttcttagca agaaagctat
     1141 attatgaagg caagttaaca tcttatgagg aatatttaga agctgtaatg tacagagaaa
     1201 ctttatctcc aacaggtttt gaaagagggc ttgcaattcc tcatggaaaa tctaagtcag
     1261 ttaaagaagc ttcatttgca gctatgacct tagagacacc aatagaaaat tgggaaagcg
     1321 tagacccaaa taataaagta gagctaatat ttttaatagc agtaccagaa aatgaaggtg
     1381 gaaatacaca catacaaata ctttctgagt tagttactag actgtcaaat gaagattata
     1441 tgaatatgtt gttaaattca aaaactaaga aagagttatt taataactta gatataaaag
     1501 aaaagaaaga acaaataaaa gaagagatta ggattcaaaa tgaatcaaaa aaaattatat
     1561 tagcaataac agcatgccct gcaggtatag cacatacata tatgtcagca gaagcattaa
     1621 ttaaagctgg taaagaaatc ggtgtggatg tgtatgtaga aaagcaagga gctaatggta
     1681 tggtggaccc tcatacacca gatataataa aaagggcaga tgcaataata tatgcaacag
     1741 atgtagcacc taaaaataca gaaagatttg aacatttgcc aaacataaaa acgtcagtag
     1801 cagctccact aaaaagagca aaagaaataa tatacgaagc tttagaagta gcacaaaaac
     1861 aaggtaaagg tgattatata gaaaaatcat ctgatatatc atattgtgaa aaatcatcat
     1921 ggaaaaaaga tgtaaaatca gcaatactta caggagtatc tcatataata ccacttattg
     1981 tggcaggagg tatgatactt gcatttgcag tattagcatc tcaagcattt ggtctacaag
     2041 aactatacaa ccaagaaggt tcttggttat ggcaattaag aaagctttct ggaggtatgt
     2101 taggtacatt gatggttcca gtattatcag catatatgtc ttactcacta gctgaaaaac
     2161 caggtcttgg accaggattt gcagcaggtc tagcagctga tttaataggt agtggatttc
     2221 ttggaggtat gttaggaggt ttacttactg gctatatagt aaaattttta aagaagaata
     2281 taccagccaa aggcacatta gctggattcg taagtttttg ggtatatcca gtagttggtt
     2341 ctttggtagt aggtttattg atgttatttg tagtaggaaa gcctgtagca gcacttaaca
     2401 atggtcttat aaattggtta aatgggatga ctggagtaaa tgcaatacta ctaggtgcaa
     2461 taataggagc tatggtttca tttgacttag gtggaccaat aaataaagca gcctatacat
     2521 ttgcaatagg agcaatagca agtggaaatt ttatacctta tgcaatattt gcatcagtta
     2581 agatggtttc agcttttgga gttacaatag ctactgtttt aggcagagat ttattttcag
     2641 aatcagaaaa ggaaatagga aagcaaactt ggatattagg attggctgga ataactgaag
     2701 gtgcaatacc atttatgata gaggacccaa tccgtgttat accatcattg gtagctggtt
     2761 cagccataac tgggtctata gtagcattct tcaatatagg tcttcaagtg cctggagcgg
     2821 ggatattctc attggcgctt ttagagggaa gtatgtcagg atttattata gcatcaatat
     2881 ggttctttgc ggcagtactt ggagctataa tatcagcaat attattaata ataactagga
     2941 aacaaaaaaa tcaaagaaag atacattaaa agatacgtta ttagatattt aaatcaaagg
     3001 tttaattata gtgataaaat aaatattaaa aaattttgta aactcagtag tatttttatt
     3061 ggatttttaa ttagacagaa aatattatca gataagtttt ggaggataag caatgaaaaa
     3121 aaatgtacac atagttcctc atatgcattg ggatagagaa tggtacttct caactgagga
     3181 atctagaata ttacttgtaa ataatatgga agaaataatg gatatgttag aaaataatca
     3241 taactatcca tattatgtga tggatggtca aactgctata ttagaagatt atttagcagt
     3301 taagcctgaa tgtaaggaga gaataaaaaa attagttcaa gaaggaaggc taataatagg
     3361 tccttggtat actcaaactg atgaaatggt agttggtgga gaatccatac ttagaaattt
     3421 actatatgga ataaaagatt gtgatgaatt tggagaatat atgaaaatag gatatttacc
     3481 agattcattt ggtcaaagtg ctcaaatgcc acaaatacta aatgggtttg atataaaata
     3541 ctcaatgttt tggagaggat gttctgaaag aaaaggaact aataaaacag agtttaattg
     3601 gaagagtgat gatgggtctt ctgttttggt tcaaatactt ccattaggtt atgcaatagg
     3661 aaaatatcta ccaatgaatg aagatgagct aagaacaaga atggataaat atttaccggt
     3721 tcttgataag ggtgcaacta ctgaccatat aatacttcca aatggacatg accaaatgcc
     3781 aattcaaaag aatatatttg aagttatata taaattaaaa gaatgttatc cagagagaaa
     3841 atttttctta agtagatatg aaaacatatt taaagagcta gaaaaaaatg aggatataga
     3901 tacaataaaa ggggaatttt tagatgggaa atatatgagg gttcatagaa gtatattctc
     3961 aacaagaatg gatataaaat cagcaaatgc tagaatagaa tcaaagataa ctaacatatt
     4021 ggagccacta gcttctattg catattctct aggatttgaa tatcatcatg gattaataga
     4081 gttaatttgg aaagaaataa tgaaaaatca tgctcatgac agcattggat gttgttgttc
     4141 agataaagtt caccaggaaa taatgaatag atttttctta gctgaggaaa aagtagacca
     4201 attaataaca ttttacaaaa gaagaatagt tgatgcaatg agttgtgaaa tggctttaga
     4261 taagttagta gcatttaact taatgcctta cgataggaat gaaatagtga gcacccaagt
     4321 tataactaaa atgaaagcat ttgaaatatt tgataaagat aataataagt tggattttga
     4381 agtggtacat aaagaagtaa tagatgcagg attaatagac cgtcaaattg ttcattatgg
     4441 aaactatgat tcatttatgg tatatacaat aaactttaaa gataaaatac ctgcaatggg
     4501 gtataaggcg tatatgataa aagaagttga acatatgtta gaaaaagctt atgaagtatg
     4561 tgacatggta gataatgact tttatactat tcaagttaat gaaaacggga cattaaagat
     4621 atttgataaa aaattaaata aaacttttga aaatgtatta ttgatggaaa atggaggaga
     4681 tgaaggagat gagtatgatt tttcaccact tccaaatgag aaattaatat ttaacgataa
     4741 tatagtagca aattccacta taaagaaaaa tagatttaat aatgagataa agataaatta
     4801 tagattaaaa gtaccaaaag atatacaagc tagaaaaaat aatagagcag attcatttat
     4861 tgactttgat atagttataa atgttccaaa tgataaagct ataatagatg tagtattcaa
     4921 cataaataac caagcaagag attgtagggt tagaacttat ataccaacaa atatagcttc
     4981 aaagttttct gtgtctgata atcagtttgg acatataaag agaagtgtgt atgatgatgc
     5041 tatggaagtt tggcaacagg aagaatggag tgaaagacca gattctatat atccaatgct
     5101 aacattcgtt ggattgtctg acaaagagca tggagtagct gttcttacaa acagtacaag
     5161 agagtttgaa atagtagggg aaaaatttga cacaatagct ataacattat ttagaagtgt
     5221 aggattccta ggtaaagaag aaatggcaag acgcccaggt cgtccatcag gaataaagtt
     5281 gcctactcca gattctcaaa tgataggaga tataaaaata gacttagcaa tagttactca
     5341 tgaaaaatct acactagaag caaatgttgc aaatatggct aaagagtatt taacaaaaat
     5401 acaaacttat aataaaatgc catataatgc tatgaaattg aatcaatcag aaatagaagt
     5461 agattattca tatagtttac ttaaggaaac taataaggac cttgtgttaa gtgttgtaaa
     5521 aaaggctgaa aaagaagagg cattagtagt tagattttat aattctaaag aagaaacaaa
     5581 atcagctaac atagtattta acaaaaatat taaaacagct aaatggatta acctaaatga
     5641 aaaagaatta gaatctatat caatagataa taactctgta aatgtagagt gcttaatgaa
     5701 ccaagttaaa actatactgg ttaaatagtg ctaaaaatga ttagatagtg ctaaaaagga
     5761 attatcttaa aatagagata atcctcctta tcattgttga aataataaac atatatttga
     5821 ttttatacaa aagagtgtct catgaactaa gaaatttatt ttgagacact ccctttttat
     5881 aagataaaag aatattaatg gtataagttg tatacaaaga gagatgtaga gtataaaata
     5941 aaagataaat taagcttata aaattatatg tttaaaataa ggagtaataa aaaggagtag
     6001 ataagtatga aaatattaat ttctatagat tctttgaaag gaagcttatc ttcaatagag
     6061 gcaggaaatg caataaaaaa ggggatattg aaggttaaag aagatgcgca agtaaagata
     6121 ttgccacttg ctgatggtgg tgaagggaca gtagatgctt tagttcaagg gatgaatggt
     6181 aaaaaagaga ctatagaagt tactggacca atagctaaaa aagtggatgc aacatatgga
     6241 ttattaaaaa atacaagtac agcaataata gaaatagctc aagcatcagg attaacattg
     6301 gttccgactg aacttagaaa tcctttatac actacaactt atggtgttgg ggaaataata
     6361 aaggaagcaa taaataaagg ctatagaaat tttatagttg ggataggtgg aagtgctact
     6421 aatgatgctg ggataggtat gcttcaagcc ttagggtttg agttttatga taaaaataac
     6481 aagttagtag gattaggtgg aaaagtttta aatgaaataa gacatataaa aatagaaaat
     6541 agattaaagg aattagatga atgtaagttt aaaatagcct gtgatgtaaa taatccgctg
     6601 tttgggaaaa atggagctgc atacatatac gggtctcaaa aaggtgcaac atcagaaata
     6661 attgaggagt tagataatgg gcttagaaat ttttccaaag tagttaaaaa ttacttaagt
     6721 aaagatgtag ctaatgtaga aggagcagga gcagcaggag gattaggttt tgctttttta
     6781 gcatttttaa attcaaaatt agaatctgga ataaaaataa tattagaaga aattaaatta
     6841 gaggaagagt taaaagatgc agattttgtc ataactggtg aaggtagatt ggacaatcaa
     6901 actgctatgg gcaaggctcc aataggagtt gcaaaactgg caaaaaaata tgaggtaaaa
     6961 gtaataggtt tggctggagc cacaacagaa gatgcagtta aatgtaatga agagggtata
     7021 gatgcttatt tttctatagt aaatagagct atgacaatag aagaagcaat ggataaagca
     7081 actgctagtg agaatatgac agctacaaca acacaaatat tcaatcttat aactagtata
     7141 caacagtcaa aaaatatgta gtattaaaac aaggtatatt aggtaaaaag ctgtcacata
     7201 ttaatttaga ctttttagtg tgacagctta tctattttat gttaataact taagaaacat
     7261 atattggaaa taaattaatt gatttagata ttttgaataa gataagctat tctattcata
     7321 aaactactat agttatctgc cttagtaagt tctttaacat tattccactc agataatatt
     7381 tcaataaagc tttcaaatat gtaattgaat ttttcttttt ctttttcatt tataggtatc
     7441 atagctataa tctgaacttt ttcctcaccc catttaactg gtttatcgtt aacaattaaa
     7501 catactccag tttttaaagc atccattttc atgctatgag gaattgctac attgttatta
     7561 aatgcagttg aagacatttt ttctcttata agaacatcat ctatataatt gggtttgaca
     7621 tatttatttt cgtacagaat atttcccata aattttatat aatctttagc actatctaaa
     7681 tatatatttt tcataaacaa agattcacta aagtacattt ctaaatgatt ttgtacagta
     7741 agtatatttt ttctatcttt aatttgagta aatttacttt gaatattatc ataatccttt
     7801 ctatttaggt aaggatttac ataaacaaaa ggtatagggc atctattgtt aattggtaaa
     7861 gtagtaataa ccatgtctac cttattgtca aaatcaagat tatgagtatc tgaaaaaatc
     7921 tgaataattt ctatggattc attaaatctc ttctcgaggt ctcttactat tttgaattgt
     7981 aaatcatagt aatttggagt aattaatgca catagaactt tatcttctaa taatttttgt
     8041 ctatcaaaga atgaacctac atgaagagat atataagata tttcatcttc gtctatatct
     8101 acattttcta aagtttgaat ctgattgcaa atataaacag atatatcata tatcaaaggg
     8161 taggaatctt ttaacttttg aggtatttga tttcttaaaa cttgattatt tttggctcta
     8221 ctgattaaat tttttacatg aagagtaaat tttacaacaa attcctcatc agataaatct
     8281 ataagatata agtcatatac atttttaatt atttttttag ttaatttaac aaaatgttca
     8341 tctatataat ttggcaatga cttagtatcc atttcatgat aatttaagac tgtggttttg
     8401 tttgttaact gaaatactaa gtagtataat tctgcactag ttaaagttat attgtattca
     8461 gactctaaaa aatttgatat atctcttgaa atacttatat cattattatt atctatgtag
     8521 tttactactt cgacagattc tatgtagtta ttctttttta ttctgtctat agttatcatt
     8581 aaatgaagaa gtatgttgtt tatagcatac tcatttataa acaaattgta tttttttaat
     8641 atatatatta tattttcttt tagtttagtc aaattatact cttgatatat tttttgtaca
     8701 tcaaataagc ttaaaaaatc acttcccgtt tctttaaata ttatagagct cattaatttg
     8761 cgctttgcag actcatttcc aattagtttc aataaaaatt tcgattgctt tatctttaaa
     8821 ttataacttc ctataaattt attagctttg actatatctc cttcaatagt agctgggcta
     8881 acaaacaatt cttcacttaa atcaaatata ttaattccat tactattaac tataagttct
     8941 tttaaaatta aatttaacct gttatcaggt gatatatttt ctgaactatc aaccttggtt
     9001 tgacaattat tattgagatt tactttatat ccttttttag aggataatat aaattcacca
     9061 ttggaattaa tttcgtttac atattttcta attgaacgcg ttgagacatt aagataatta
     9121 gccaatgtag atgaatctat ccaatcatgt tgtgttttta tgaaatttaa aaatagttct
     9181 tttttagatg ttttcattag attccctcgc atagttttaa aataaaatct ggatgatatg
     9241 ataattatat aattgaatgt acgtattata agatacatta tactatatta atatgtttaa
     9301 ttaagagtaa ctgatttcct attattagga aatatattta tgtgaaatga acttatttgg
     9361 taagtacaat ataaacataa taaaataaaa agttcattaa gaaataaatt aatggataaa
     9421 aatattggag gtattattat gaaaaatata agagtgcttt tggcgtgtgg ttcaggagca
     9481 agtacaggtt ttatggcaac tagtatgaga aagagtgcaa agaaaaaagg tatagaagca
     9541 gatataaaag cgagaagtga gtcagagata gataagtatt tatcagaaat agattgttta
     9601 ttactaggac cacatttaga ttatatgaag gaagaaattc aacaaaaggc taatgaatat
     9661 aacgttccag tagcatgtat atctaaaact gcatatggaa gactagatgg tgcaaaggct
     9721 ttggaagaag cactagaact aatatcaaac aaataaaata tttatgggga gagtgtttat
     9781 gaataaattt atagattttt taagtgaaaa atttactcct gttgtaaata atatgactaa
     9841 gaatatatgg gtacaatcag ttcaatcaac tataatgaaa gttttgccaa tggtatttgt
     9901 aggctcatta gtaactatag tttctgtctt gaagaattac ataagctttt tacctgattt
     9961 aagtccaata aatcaatata catttggact tttaggatta tttatagcat ttttattgcc
    10021 tatggaaata atgaagaata agaaatttga aagtatgagt gtagtagctg gtcttgcagg
    10081 tgctggatta atgttaatga tgattcgtcc agagattacg aatgaaggag ctatatttaa
    10141 cttcaacagg tttggaggag aaggaatgct tgtatcgcta gttgcaggat tattctctgg
    10201 attaataatg tctttatttg gttctttttc atttttcgga gaagattcag cacttccgga
    10261 ttttgtaagc aagtggtttg ataatatgtt acctataaca gtgataatgt ttgtaggctg
    10321 gggattaatt tatggactca actttgacat gtttgaaata atagttagag tatttaaacc
    10381 agtatcagat atagcacaga cattacctgg atttgtactc ttaaacttct tagtagtatt
    10441 tatatattct ttaggaataa gtgggtgggt actatctcct ataacttatt caattcaact
    10501 tggtgcaatt gcagctaatg cagcagcagt agcatcagga ggagtagcaa ctaatatata
    10561 tgtttacgaa gttattcaaa caggttggat aactttagga gggttgggtg ctacattacc
    10621 attagttata atgatgtgtt tatccagagt tggaagaata aaagcaatag gaagagcatg
    10681 tattgtccct tctataatga atataaatga accagtaata tttggagctc cagtggcatg
    10741 gaacccattt ttaatgttac ctatgtggat aggaacagta gtaatatcaa taactactta
    10801 cttatcaatg agtatgggat ttgtgacaat tccaagtaag gtgtttaatt tatggtatgt
    10861 tccttttggt atatctactt ttatggtaaa tcaagatgtt agaggtctta ttgttttagc
    10921 aataaatata gtattgctat ttatgatatg gtatccattt tttaaagctt atgagaaaga
    10981 agagataaaa ttagagagcc aagaggttta aatttagaaa ttgaaaggaa attataaata
    11041 tgacaattga agaacatata atagatgaag aattagtaga ggtggcaatg caaataatac
    11101 ttcgtgcagg tgaggctaga acagaaatta aacatgctct taatgatttg gaaaagtttg
    11161 attatagaaa tgctgattta aaactagcta aagcaaaagg gtttatgaca gaagcacata
    11221 gagctcaaac caagattata caaggggaag ctggtggaga gaaaagagca cattcgctgt
    11281 tatttgcaca tgctcaagat actcttatga ccatattcag cgagttaaat ataactatgt
    11341 ctttagtagg aatagtaaag tctatagaat ataaggtaaa taatagtaaa tgatttaaat
    11401 aaataaaata aggagtgatt aaattgggta agtttcctaa aggtttttta tggggaggag
    11461 ctgtagcagc taatcaatta gaaggtgcat ggaatgaagg tggcaaaggt tggtgtttag
    11521 ctgatgtaca tatgtatgat aaagatagag atatatctaa accttatgag tctgatatga
    11581 ctatagaaag aataaagttt gctatgaaag ataaaaatgg gtactatcct aagcgtagtg
    11641 gaatagactt ttatcataca tacaaagaag atttaaagct tttgaaagcg ctagggttaa
    11701 agtgttttag attttcaata aattgggcta gaatatttcc aaatggagat gatttagagc
    11761 ctaatgaaga gggattaaag ttttatgata atcttataaa tgaagtaata gataatggta
    11821 tggaacctat tgtaacacta cttcactatg agactccttt gaatttagta ttaaaatata
    11881 aaggatggtc taatagaaaa gtggtagatt tctttgttag atatgcagaa ttaatactaa
    11941 atagatataa aaataaagtt aaatattgga tagttataaa tcaaataaat ttaatacatt
    12001 atgaatcatt taattcagtt gcattttgtg tagaccaagt agataatgta gaagaggcta
    12061 gatatcaagc tattcatcat caattcatag ctacagcttt gactaagaag cttggaaaag
    12121 aaataaattc agatatgatg atagggatga tgatggcaga ttgtacatct tatccagaag
    12181 attgtgaccc tgaaaatgta gtatttgctt taaaaagaaa taggctacaa tattttttca
    12241 ctgatgtaag ttttagggga gagtatccag tatatattaa gagatttttc aaagaaaata
    12301 acataaatat agttatggag gaaggtgatg ataaaatctt aaaagaaaat actatggatt
    12361 ttctagcttt atcatactat tattctacaa cagtatcagc aagtaaaaac accatgaatc
    12421 caactgacaa tataaagaat ccatatttaa aagcaaatcc ttgggggtgg gcaattgacc
    12481 caaaaggatt atacaatact ttatctcaat attatgatag ataccaaaag ccaataatga
    12541 tagctgaaaa tggatttgga atgcatgata aatttgaaga tggaaaagtt catgatagtt
    12601 acagaataga ttatttagga aaacatatag aacaaatagg tgaagctata gctgatggaa
    12661 ccgaaatatt agcttattgt atgtggtcac caatagatat agtaagttgt tcatctcaag
    12721 aaatggaaaa aagatatgga tttatatatg tggatataga taacaatgga aatggaagta
    12781 aagaacgtta taaaaaagat agtttttatt ggtatgaaaa tgtaataaga actaatggag
    12841 aagaattaag agaaaagtaa atagttttta aatataaaca acccccctta taaatactgt
    12901 atatattatt aatatagttt tataaggggg gttataattg taaataaaat gatttattaa
    12961 ttatatgatt catagcgaaa tttatattta tacttttaat cataaacata tcagtatgag
    13021 gaatatctat tgtacttcat ccaaattttg gtagaggtac aattcttttt tattttaaaa
    13081 tgctataaga taacataata aactttaagc aaaaaattat tatattataa atttacatat
    13141 atagacattg actaaatctt aattgtatat aaaaataagt agttaagagg taatatatta
    13201 tattactcga taaatttaga caaataaaac ttatataagg ggagataaca tatgataatt
    13261 gagctaaaca aaagtaattc tgaaaatcta acgaagacgg aacttgaaat aattaaattt
    13321 ataaatgaaa atgaatcaat attaccagaa ttatctatag tagagattgc tcatgaaaca
    13381 tattcatctc cagcaaccgt ttcaagagca attagaaaat gtggtttaaa tggattcaat
    13441 gaacttaggt atagattaac agtaaaagat gaaaatgatg atatacaaaa tatgggggat
    13501 ataataaata aatcctttat agaagcacag agagttatag aacaaatatc tttaactaca
    13561 ctaatcaata taataaaaac tataaataaa tctagtaaga tatatatttt ggcaagaggg
    13621 cttactgaat atgtagcaaa agagtttagt cttaaattgc aattacttga ttttaatgtt
    13681 gtttttataa atgaccccaa cataatgaaa ataaaatcta aatcaataaa aaaagacgaa
    13741 cttataatta tattttcatt gaatggaaat accatagagc ttgtagaatc agcacagaat
    13801 gccaatttag ttggagctaa ggttataact tgctgttgta gtgaaagtgc aaaacttata
    13861 aagtattcgg atttatatat tgtaggatac aaacatgaac atatatcaat ttctgaatat
    13921 gaagtatctt ccagattgcc cttgtatatt atatctagga taattataga ttatatggtc
    13981 agatataata attctgaaaa ttaaattgaa aagtctatga aaatcgattt tctatttgtg
    14041 aaaagatttt catagacttt tttattataa tgaaaacata cacagcacga gcaaggggga
    14101 agacaatgat atatacatta actacgaatc ctgcaataga tatgaatata tctactaatg
    14161 ggataaagag aaaattagta aatcgtacat caaatgctgt atattctcct aatggaaagg
    14221 gattaaatgt aacttttgta ctaggtcatt atggtataga atctaagata ttaggatttt
    14281 ttggaggatt ttcaggaaaa tatatagttg aagaaagcga aaagagagga tttgacgtat
    14341 tgcctacttg ggtagaagat acaactcgta taaatatatt tttaaatgat ggaagtgatg
    14401 aatttaaatt tgtaaactct gggtcatatg taaatgaaaa acaaaaacta gatatgctag
    14461 ataagataca gtcattagaa gatataagtt acttatcaat aagtggaagt ctacctcctg
    14521 gtattgatga taattactat gaggatatat ttaaaatttg taaaaataaa agtataaaga
    14581 caatactcga tataagttca ccaaaattaa aggagttact acagtataat ccatatttga
    14641 taaaaccaaa tgatgaagaa atcaaagata tatttggaat tattgtaaga gatgaagaag
    14701 atattaagga tgtacttaaa ttactacatt taaaaggtgc tcaaaatata ttgctaactt
    14761 taggagaaaa aggttcttat ttttataatg gaaaatcaat ttattatgct agtgctcaac
    14821 cagttacagt agtcagctct gcatgtgctg gtgattctgc attagctgct tttttaagta
    14881 tatggttgga aaatccagaa catattgaag aagcattaaa aaggtcagca gcaacaggag
    14941 caagtgtggc tgaaagtaat ggtataggta atttggagaa tgtggaagat tatataaaaa
    15001 atataagagt cagaaaggtg gtataaaaat gggaaagaaa atgattctag gggtaacagg
    15061 ttgtccaact ggaatagctc atacttttat ggcagaagaa gcattaaaaa attcagccag
    15121 ggaattaggg tgtgacataa aagttgaaac gaatggagct ataggtgtag aaaataagtt
    15181 aacagcaaaa gatatcgaaa tggcagatgc aataatagtt gcttgtgata aaaatgttga
    15241 tatggacaga ttcaatggaa agccagtaat agaagtacct gtcaaagaag gtatacataa
    15301 agctaatgaa cttatacaaa gatgtattga tggaaaagta gctattagaa aaggaaactt
    15361 atcaagcagt aaaattgcag aagaaggaaa tttatcgttt ggtcaaaagt tgtataaaga
    15421 cttaatgaat ggtgtatctc acatgttacc tttagtagta gcaggtggag tacttactgc
    15481 tatatcattt ttatggggga tttattcatt tgacccaaat tcagaacaat ataatcaaat
    15541 agcagctaca ttaaaatctg ttggtggata ttctatgaac ttgatggttc ctgtattggc
    15601 agcatttata gctcaatcta tatcgggaag agctggtatg ttggcaggac ttgttggagg
    15661 aatgatatca tttgatacag gttcggggtt tttaggtgga atcatatcag gatttttagc
    15721 aggttactta gttaaactat ttgtacattt gttaagaaaa ttaccacgac aattagaagg
    15781 tcttaaatcg atttttataa ttcctattgt cagcgtaggt cttgtgggtg tagcaatgtt
    15841 gttacttggt gggccatgtt cagcacttaa caatgctatg atgaactttc tatcggggtt
    15901 acaaaattca agtcctataa ttttgggaat agtaatagga tgtatgtcag catttgatat
    15961 gggaggtcca gtaaataaag cagcatatgt aactggaact atgcttctag gacagggaaa
    16021 ttatttattt atggcaggtg tatctgcagc ttgtataaca ccaccattgg taatagcaat
    16081 agcatctact ttatttaaaa atagatttac agaagaagat agagcagcag gattaatcaa
    16141 ttatatacta ggtagtactc atataacaga gggggcaata ccttttgcag caaaaaatcc
    16201 attaaaagtg ttgccagtac ttatgattgc atcatcaata tcatcaattt taacttatat
    16261 aatgaaaata gaagtaccag ctccacatgg agggtttttg gttctaggat tagtcaataa
    16321 gccactttta tgggtagggt gtatatttgt aggttcatta gtaggtgctg tactttatat
    16381 gatggttaca cctaaagtag taaatgagaa aaatactata gaaaatacaa atgcaacaaa
    16441 agatgaacat aaaaatgaaa gtgcagaatc ggaaaacgta tctaataata tctctttata
    16501 tagcgaagaa acagtaattt tagatgttaa gggaaaaaat aaaacagatg taattgatga
    16561 aatgattgga atacttgata aatctggtgt tcttttagat aaaaagaagt ttaaagaaga
    16621 aatccttaaa agagaagaaa tatcatctac aggttttggt atggggatag cgataccaca
    16681 tgcaaaaaca gatgcagtaa aggtaccacg tgtagcagta ggtgtatcaa aagaagggtt
    16741 tgactttgaa tcagaagatg gaagcttggc acatattata tttatgatag cagctacaga
    16801 taatggtgat aatttacact taaaaacatt atctcaacta tcttcaaaat taatgaatga
    16861 agaattttta aatgagttat taaatagcaa aacaagtaga gaaatagtat caaaattaaa
    16921 taacgaagaa attaaatcag tataaacata taggaggaga aattatggca ttagtgacaa
    16981 ctaaacaaat attattagat gcacaagaag ggcattatgc agtaggtgca tttaatgttg
    17041 aaaatatgga gatggttatg gcagtaatag aggctgctga agaacttaaa tcaccagtaa
    17101 tattgcaaac aacaccttca acaataaagt atgcaggact agattattat ttagcgaatg
    17161 taaaggtagc atcagagaag gcaagtgttc ctgtggcaat gcacttagac catggttcaa
    17221 gctttggact agctatgcag gcacttagag ttggatatac ttctattatg attgatggtt
    17281 ctcatgaaag ttatgaagat aatatttcaa tatctaaatc tgttgtagat gcatgtatgc
    17341 cttcagatat acctgttgaa gctgaattag gtaaagttgg aggtaaggaa gacgatttag
    17401 atggtggtga tgggggagca tatacagacc cattagaagc aaaagagttt gtagagagaa
    17461 caggggtatc atctttagca gtagcaatag gtacggcaca tggattatat aagggagagc
    17521 caaagcttga tttagataga ttatctgaaa ttagagatgt tgtatcagtt ccattagtat
    17581 tacatggtgg gtctggaata ccagatgaag ttattaagga atctataaaa agagggattt
    17641 gcaaggttaa ttatgcaact gaattaagga ttgcttatag caatggagtt aagaatgtgc
    17701 ttaattctga cccagaggta attgacccta agaaatatgg taaaaaaggt ttagagtcag
    17761 ttaaagattt tgttaagagt aggatggaag tttgtggatg tgtaggaaaa gcaatagaga
    17821 cagtgaagtg ttaataataa agggaaagaa taagtaagat taaattaagg ggtgtctcaa
    17881 aatgaacttt ttagttcatg aggcactctt ttttgtatga aattaaatat attttcatca
    17941 ttctgacaat aaaaaagaag attatatcta ttttgagaca gcatcttatt ttattttttg
    18001 attaatattg taattgagaa atttgaaata aggaagatga tataattaaa taaaaaagtt
    18061 ctttctaagt ttaaattaat attaaaaagt tataatatg
//
LOCUS       DAELNY010000069        17793 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.069, whole genome shotgun sequence.
ACCESSION   DAELNY010000069 DAELNY010000000
VERSION     DAELNY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.069"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..160
                     /locus_tag="KPE55_001977"
     CDS             <1..160
                     /locus_tag="KPE55_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493045.1"
                     /translation="ITNTYVFKQLTLNNSTKERRKDIIEKIYNSVDYGTNKLDENMFF
                     TGGIKYVK"
     gene            150..470
                     /locus_tag="KPE55_001978"
     CDS             150..470
                     /locus_tag="KPE55_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493046.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            668..1537
                     /gene="galU"
                     /locus_tag="KPE55_001979"
     CDS             668..1537
                     /gene="galU"
                     /locus_tag="KPE55_001979"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0493047.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            1561..2574
                     /gene="galE"
                     /locus_tag="KPE55_001980"
     CDS             1561..2574
                     /gene="galE"
                     /locus_tag="KPE55_001980"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG0493048.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            2686..4620
                     /locus_tag="KPE55_001981"
     CDS             2686..4620
                     /locus_tag="KPE55_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG0493049.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            4775..5638
                     /locus_tag="KPE55_001982"
     CDS             4775..5638
                     /locus_tag="KPE55_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG0493050.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(5846..6727)
                     /locus_tag="KPE55_001983"
     CDS             complement(5846..6727)
                     /locus_tag="KPE55_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0493051.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            7140..7988
                     /locus_tag="KPE55_001984"
     CDS             7140..7988
                     /locus_tag="KPE55_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG0493052.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            8067..9989
                     /locus_tag="KPE55_001985"
     CDS             8067..9989
                     /locus_tag="KPE55_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0493053.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            10071..10934
                     /gene="aroE"
                     /locus_tag="KPE55_001986"
     CDS             10071..10934
                     /gene="aroE"
                     /locus_tag="KPE55_001986"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0493054.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            11134..12351
                     /locus_tag="KPE55_001987"
     CDS             11134..12351
                     /locus_tag="KPE55_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0493055.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            12587..14452
                     /locus_tag="KPE55_001988"
     CDS             12587..14452
                     /locus_tag="KPE55_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG0493056.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(14630..15994)
                     /locus_tag="KPE55_001989"
     CDS             complement(14630..15994)
                     /locus_tag="KPE55_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493057.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(16173..17516)
                     /locus_tag="KPE55_001990"
     CDS             complement(16173..17516)
                     /locus_tag="KPE55_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0493058.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(17589..17690)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         6804 a         2123 c         3120 g         5746 t
ORIGIN      
        1 aataacaaat acatatgtat ttaaacaatt gacattaaat aatagtacaa aagaaagaag
       61 aaaagacata attgaaaaaa tatataactc tgttgattat ggaaccaata aactagatga
      121 aaatatgttt ttcacaggag gaataaagta tgtcaagtaa atacaatgtt tggactttta
      181 actatgagtt cttaggattg gactcagggg atgaaaaaaa taatgaagtt tattacgaga
      241 ttgatttaga tgaaatttta aatgaaaact atatagatga agatatagat agtggtttag
      301 attctgatgg aaatagtatt ttaggagaat taatatcaga aatggatata gatgaggatg
      361 tcagtgttga tatgacatat caagaactag aagttgactt agaagattta gattcttatc
      421 tagatgaaga tatagatagt gatataaaag gcatcttaga tgaaatttag tacctgtata
      481 agttttggat aaaatataaa caatacaata ttaacttata aaactaaagg tagatgaaat
      541 ttcatctacc ttatattatc ttatagcatt aaatattggg ataatataga gtttgaagaa
      601 aaagtataaa tatggtatat taatttggta caatagaaaa tttacgaaga agggtgaaaa
      661 aatgcagatg acagttaaaa aagcaataat accagcagca gggctaggaa ctagattttt
      721 accagcaaca aaatcacagc ccaaagaaat gcttcctata gtagataaac caactttaca
      781 gtatataata gaagaagcta tagaatcggg aattgaagaa attcttataa taacaggtag
      841 aaataaaaaa tctatagaag atcattttga taaatcagtt gaattagagt tagaactaga
      901 gcaaaaaggt aagacagaga tgttagaaat ggtaagagac atatcaaata tggtaaatat
      961 tcattatata agacaaaaag agccaaaagg tcttggacat gcaatttact gtgcaaaaag
     1021 ttttataggt gatgaacctt ttgccgtatt attaggtgat gatatagtag attcagatgt
     1081 tccgtgctta aaacaattaa tagatactta taatgagtat aaaacaactg tattaggtgt
     1141 tcaaaagata gctaaagaag atactaataa atatggtata ttggatgtaa aacatatcga
     1201 agatagagta tataaagtta aagatatggt tgaaaaacct gcaattgaag aagctccatc
     1261 tgatatagct atacttggaa gatatataat aactcctgca atatttagca tattagagaa
     1321 acagacacct ggtaaaggtg gagagataca actgacagat gcacttcaaa ctcttggaaa
     1381 acaagaggct atttatgctt ataactttga aggtagaaga tatgatgtgg gagataagat
     1441 tggtttttta gaggcaacaa tagactttgc tctaaagaga gaaaatttaa aagatgattt
     1501 aatggactat atgagaaaaa aagtaagtga aaaataaata ttagaaaaga ggtaattaca
     1561 atggcagttt tagtagctgg aggagcagga tatataggaa gccatacagc aatagaactt
     1621 ttagaatcag gttatgaagt tgttatagtt gataacttaa gtaatagcaa ctcaatagtg
     1681 gttgatagaa tcaaggaatt atctaaaaaa ccagtaaaat tttataatat tgatatcaga
     1741 aataaagatg aaatgcatgt tgtatttaaa gaaaataata ttgaatcaat tatacacttt
     1801 gcagctttaa aagcagttgg tgaatcagta gaaaaaccaa ttgaatatta cagcaataat
     1861 ctaataagta ccttaaattt atttgaatta atgagagaat atggtgttaa aaagtttgta
     1921 tttagttctt cagctactgt atatggagac ccacatactt gtcctatatt agaggatttt
     1981 ccactttctg taaccaatcc atatggaaga actaaactta tgatagagca gatgttagta
     2041 gatatatcaa aagcagataa gtcattggat atagctcttt taagatactt taatccagtc
     2101 ggtgctcata aaagtggtag aataggtgaa gaaccaaatg gagttccgag caatctaatg
     2161 ccttatataa caaaaatagc agtaggtaaa ttgaaggaat tgagtgttta tggtaatgac
     2221 tatccaacac atgatggaac tggagttaga gactatatac atgtattaga tttagctgct
     2281 ggtcatgtaa aagcattaca aaaattagaa gaaaatccag gacttgttgt ttataattta
     2341 ggaactggaa aggggtatag tgttcttgat ttagtaaaag cattttctaa agcaagtgga
     2401 aaagaaatac catataagat tgtaggaaga agggctggag atgtagccat gtgttatgca
     2461 gattcttcaa aggcagaaaa agagcttgga tggaaagcta agtatgaact tgaagagatg
     2521 tgtgaagatt catggagatg gcaaagtatg aatcctaatg ggtatgaaga atagaatata
     2581 tacttatatg tataaatgta acaaatttgt aaatataagg tattggtaat tttatgttat
     2641 aattagacac atgtagtaat gtttctaaaa gagggggaag ttatagtgaa aaaaagtcta
     2701 aaaaagtatt tagtactagc actgacttta gttttagtat ttgcatgtgg cgtaccagaa
     2761 tcaagcgcag ccagtaaaca tgtgattata gtaaattcaa gaaaaaatac tctaggatat
     2821 tttgtaaata ataaacttgt aaaagaattt agagttgcaa caggtaaaaa ggggtcagaa
     2881 acaccaacag ggaaaacaaa agtagttaac aagataaaaa atagaccata ttataaaggt
     2941 aatatacctg gaggaagtcc aagaaatcca ttaggagata gatggatggg attagcatta
     3001 aaaggaactt atggagatac atatggaata catggaaata ataatgagag ttctatagga
     3061 aagcatattt ctggtggatg tataagaatg cacaataaag atgttagatg gttatttgac
     3121 caagttcctg taggttcaga tgtaattata gattatagta atgacagcta tgtaaagata
     3181 gcggcaaaat ataagataaa tctaaatcaa acaggatgga aaacagaaaa tggtaagaag
     3241 tattatgtaa aatctgatgg aacataccaa aagaatagtt ggctaaaagt aaatggaaaa
     3301 atgtattact ttgatgcttc aggagtaatg caaacaggat ggaaaacaat taataacaaa
     3361 aaatattatt taggaactga tggagcaaga gtatctggat ggaaagtaat agacggaaaa
     3421 acttattact ttaactcaga tggagtaatg caaacaggat ggcaagaaaa gaatggaaag
     3481 aaatactatc taggatctga tggtttagca gtaacaggat ggcaagaaat agatggtaat
     3541 aagtattact ttgacaaaac aggaataatg cagacaggat ggcaacaaat agatggtaag
     3601 tcttactatt tagacaaaga tggaaagatg ctaactggtt ctcaaaaaat agatgggaaa
     3661 gattatactt ttaatgaaga tggaactata aatcctacat gggatacaat tataggtgca
     3721 aatagatttg atacagctaa aaagataagt tcagttggaa actggaatgc tgatagctca
     3781 gatactgtaa tattagtaaa tggtaatgca atagctgatg gtataacagc aactccttta
     3841 gcaagtagct atgactcaac aatactactt actaatacag ctaatttacc aactgaaact
     3901 gttgaagaaa tgaagttatt agcacctaaa acagtaatct tgataggtgg agaaaatgca
     3961 ataagttcta aattagagca agaaataaaa actactttca acacagaaac aaagagaata
     4021 gcaggtcaag atagatatca aactgctaca agaatagctg aagaacttgg aaatcgagaa
     4081 gaaataaaaa ctgcatatat ggtttctgga aatggggagg cagatgcttt atctgtagct
     4141 tctaaagctg gggaagaaaa acaacctata atattagtca ataaagatgg aattacagaa
     4201 gaatcttata aatggctaac agaaagaaaa ttagaaaatg catactttat aggtggacca
     4261 agtgcaatta acgattcagt aatagcaaaa atgaatgata taactactga agatatttca
     4321 ggaaatagaa tatatggaga tagtagagta gatactaatg ccaaagtaat agaaaaattc
     4381 tatggagata ctgatttaca ggctgtatta gttagtaaat cagatgcttt agtagatgct
     4441 ttatcagctg gaccactagc tgtaaaatta cattcaccaa tagttttaat ggataattca
     4501 ggattatcat cagagcaaca aagagtattt gcaaataaaa aagttgaaac tccttatcaa
     4561 ataggaggcg gagtatctta tatagtaatg gataaattaa tggatattct tgcaaaatag
     4621 taaaaatata tattgaaaaa taattataat tgagatgtga aattcaatca ataaaagctt
     4681 gattgtttta catctcaatt ttttagtata aaattaagtt taagttatat aaaaatcttg
     4741 gaatattttt aaaactcata taaaatgaat atagatgcta tcatcatatg ttaagagggt
     4801 gagtaagatt ataaagaaaa aatatataat gtttattgat gaaagtggaa gagctgatat
     4861 aaatcatgaa gacccattta cattgacagg agtaattttt gaatataaat atgcagtaag
     4921 tcaaggcgat tatgtatgtt ctttgaggaa agaaatggat gcatttaaag agtattgttt
     4981 tggaactagt aacataggaa tacatttgac agatatatca agaaaatcaa gagcatttga
     5041 gaagtttgat gattcacaga ttaagttatt ctatgatgag ttgcctttat ttctatctag
     5101 actagagtgt actataatat cagtaacagt agataaagct agattaaaga aatattatgc
     5161 accttcaaag gaaccgtatg ttgtggcttt tatacatgtt cttcagaatt tttattcatt
     5221 tgtaaataat gaaactgtag aatctgcacg aatagttata gagtcaaggg atgatgtctc
     5281 aaacttaaaa gtacaaaagg cgttttttga tgtatttaat aatggaacaa tatacttaga
     5341 tatagatgaa gaattaagag ataaagttaa gggttttata attgccaaaa aagaagatgc
     5401 tgactataga gaaggtttag aaatagcaga tattttatgt aatcctctta gcagagcaag
     5461 aagagggtta atagaagcta atccaaagtg tatgcaatat ggagaaaaaa acaaaatatt
     5521 caaatctgtc agaaacaaaa tatatactcc aacgagtgtg aatgatataa gaaattgggg
     5581 atttaaagaa gttcctattg tagagcctgt tggaccatgg cttgaaaaaa tgcgttagat
     5641 acaattgaca aattttaaaa aatctgatat aatataagta acctagttat tcaggaactg
     5701 aatccagttg ctgttgtata acgataatta ctgagactcg gatgccgact gtcagttagg
     5761 aatctatact tttaaaagta tagatttttt tattttataa aaagaataaa agaggatatt
     5821 taaaaataac ccctttatta ataaattata attgagcgtt tttaatgaca aagtttttaa
     5881 agtttttaat tgcaggaggc aaatatctat cttttataaa tgctaagtaa aagtttcttt
     5941 cccaacttgg gtgaataatc tgaattactt ttacttttgt aaagctaagc atattcatat
     6001 ttgggactac agcaattccg aaattttttg caactaatcc agcaattact tggtcttcct
     6061 ctacttcata gactatatta ggctgttgat ttattttttt aaacatatca tcaattatat
     6121 atctaagacc gcttctctga ttaaatacta tttgaggata aggtatggtt tcttttaaat
     6181 ctatagtatc ctttgcagct agtggatgac tatatggtac aattagtact aaatcttgtt
     6241 tggcaacagg tataaattca attccctttt ctttttcaag tttcgaacaa aatgcaatat
     6301 catattttct ttctttaaga ccttgaataa tgtcagtagt tacaccagta tgaaatttaa
     6361 aatctattga tttagcagga ttagatttta aaaatttgtg cactatgtct gggataaaat
     6421 cagtaccaag tgttctaaga aaagctaagt ctatttgtcc ttcaccagta ccagttattt
     6481 ttaatgattt tacacttgaa tctaaaattt ctaatgattt ttccacatct tttaaaaata
     6541 tttttccata cttagtaagg actatatttc taccttcttt ttcaaataga gccaccccaa
     6601 gttcattttc tagtaaagaa atagcatgac ttaagcttgg ttgtgttata gataaatttt
     6661 ctgccgcttt tgtatagtgt tctaaatgtg ccaatgtaac aaaatagcgt aagtgatata
     6721 aattcaattt ttgttcatcc tttcataggt atttttattt atatatttgt agaagttaac
     6781 ctttaagata tttttacatt tttattatat atgattatag agtaagaata aaagctttta
     6841 aaaaaattta tcgttttttg tctgaattat aagataaata tatttatacg acctaataga
     6901 aaaatacaag aattatctta taaataaaat ctataaacaa aatctatgaa ttaataggaa
     6961 attatcaatt tgttattagt aaagtctcgt gatacaataa atttataaaa tctagataaa
     7021 tagaaatcaa aaaatacttt tatttttaag gtaaaataat actactagta ggataaataa
     7081 aaatataaaa tcaaaaatac tattaattat aaagaaaata aagggggcaa atttaagcta
     7141 tgaataatca aaaaaatctt aatattccaa ttacaattag ttcttataca ttgggaacag
     7201 aagtttcatt ccgtgataga gttcgtattg caaaagaagc tgggtttgat ggtattggat
     7261 tgcgtgctga aaattatatt gatgcaaaaa atgcaggtgt tacagatgaa gaaatgattg
     7321 caattcttaa tgaatatgat atgaaagtta cagaagtaga atatataact caatggggaa
     7381 gagaagaaga tagaacggaa gctcaaagag aaaaagagca aaatgtttat catatggctc
     7441 acttatttaa tgttaaacat ataaattgtg gattattaga gatgatacca gaagaacaaa
     7501 ttataactgc attgggagaa ttatgtgaca gagcaggtga acttattata ggtcttgagt
     7561 ttatgccata tagtggtgta ccagatttgg ctactgcatg gcgtgttgtg aaaggatgta
     7621 atcgtgaaaa tgcaatgctt atattagata cttggcactg ggctagagca aagcagacag
     7681 cagaggacct tgcacctgtt ccagcagata agatagtatc aattcaagtg tgtgatgttt
     7741 tagaaactcc ttatgaaaaa cttagggatg aatcactaca tgaccgtttg gctccaggtg
     7801 aaggatatgg aaacacagag gaatttgtga aaataataaa agaacacaat atatcaccaa
     7861 gagtcatagg ggcagaagta atatctgatt ctctaatatc taaagggtta tccactgctg
     7921 ctgaaactgt atttaatgca actaaaaaag ttttggataa agcatggtct gaagtatctc
     7981 caaaataaac ttatataata taaaaatata cagagtatta taatataaaa cttttaaata
     8041 taaaacttga aaggaaggaa aaccttatga agattagttc tatgtttaca cctataagaa
     8101 ttggttcaat gacagttcca aatcgttttg tagttcctcc tatgggaaac aattttgcaa
     8161 acacagatgg tactttaagt gaaacttcta aggcttatta tcttgagcgt gctttaggtg
     8221 gttttggatt aataactata gaatctagtg ttgtagacaa aaaagcaaaa ggaggtcctc
     8281 gtaagccttg tttatatgat gatagcacaa tagatagttt taaaaatgta atagatgctt
     8341 gtcatgatgc aggttcgaag gtatctatac aattacaaca tgctggttct gaaggaaatg
     8401 aaaaagtagc aggacatcca ttaaaagctg cttctgcaat acctgcatca aatggtcgta
     8461 atacaccact agcaataaca acagaagaaa tatatgaact tatagaatct tatggagatg
     8521 ctgcattacg tgcacaaaaa gctggtgcag atgctgttga agttcactgc gctcatggat
     8581 atctggttag tagttttata tcacaaagaa caaataagcg tgtagatgag tttggtggat
     8641 gttttgaaaa tagaatgaga ttacctcgtc ttatcattga aaatatacgt aaaaaagtag
     8701 gaaattctct agcaatatta tgtcgtataa acagcactga tgatgttcca ggaggaattg
     8761 atgtacacga tagttcagta atagcagcat atctagaaga ttgtggaata gatggacttc
     8821 atgtttcacg tagtatacat atacatgatg aatatatgtg ggctcctacg actttacatg
     8881 ctggattcag tgcagaatta gttactgaaa taaaaaaagc tgttagtata ccagtaataa
     8941 cagttggacg ttatacagag ccacagtttg ctgagttaat ggtcagacaa ggaagatgtg
     9001 atttagtagc attcggtaga caaagcttgg ctgacccaga aatgcctaat aaagctaaaa
     9061 atggaaaact agacgaaatg ataccatgta ttgcatgctt acaaggatgt gtaccaaata
     9121 tgttccaagg caaacctata gcctgtcttg caaatcctat attaggacat gaagcagagc
     9181 ttaagccagc tgaaattagc aaagaagttc ttgttgttgg tggaggtgtt ggaggtatgc
     9241 ttgcagcatg ggtatgtgct aaaagaggac ataatgtaac tttagttgaa aaatctgaag
     9301 ttcttggtgg gcaaatgcgt ttagctgcat atcctccagg gaaaggagat attactaact
     9361 tagttagaag ttatatttca aaatgtaatc aatatggtgt aaaaatatgt actaatacag
     9421 aagctacagt agaacttata aaggagaaat cacctgatgt agttattata gctactggag
     9481 caactccact tgtacttcct ataccaggaa tcaatgattc aggattaatt catgctgtag
     9541 accttcttga tggtaaaaaa tcttgtggga aaaaagtact tgttgttgga ggtggaatgg
     9601 ttggatgtga agtggcagcc ttccttggag aacaagaaca tgaggttact gttattgagc
     9661 ttagagaaga agttggtgca gatgtaattt cagaacaccg taaattcctt atgaatgatt
     9721 ttgaaaatta taatatacat accattactg gtgcaaaagt ttctaaattt tatgatgatg
     9781 gtgtcgacta tttacttaca gatggcacag agcatgattt aaaaggtttt gattcagtag
     9841 tacttgctat gggttctcgt aattacgacc cattaagtga agttataaaa gaagttgtaa
     9901 aagaaactta tattgttggt gatgcagtaa aagctcgtag ggcattagat gcaacaaaag
     9961 aagcttttga agtagcatta aatatataaa taataaataa atttttcttt gtaaataact
    10021 gaataggtta tttgcaaaga aaatgataat attgagagga gattatagtt atggaaattt
    10081 caggtagaac aggattattt gcattaatag gtacaccggt tggtcactct aaatcaccag
    10141 tgatgtataa ttatagtttt aagaaattag atttagatta tagatattta gcctttgata
    10201 tcacagtaga taaggtaaaa gaggcacttt tagcaataaa aacttttaat ataaaaggag
    10261 ccaatgttac tatgccatgt aaatctgctg ttacagaata tatggatgaa ctttctccag
    10321 cagctcgtat aattggagca tgtaatacaa ttgtaaatga caatggaaag ttagttggtc
    10381 atataacaga tggagtaggc tatgttcgta atttaaaaga aaatggggta gaagttaaag
    10441 gtaaaaagat tacaattatg ggtgctggtg gtgcagcaac tgctatacaa gtacaatgtg
    10501 ctttagatgg agcaagagaa atatctatct ttaatcctaa ggatgatttt tataaaagag
    10561 cagagcaaac agttgagaat ataaaaaaag atgtaccaga atgtgttgtt aatctatatg
    10621 atttagaaga tacaaacaaa ctttatgagg agatacaatc aagtgatata ttgacaaatg
    10681 caactcttat tggtatgaag ccttatgaca atgaaacaaa tataaaagat actagtgttt
    10741 taagaaaaga tttagtagta acagatgtcg tttataatcc taagaaaaca aaaatgattg
    10801 aagatgctga agctaatgga tgtaaggcta ttggtggatt aggaatgtta ttataccaag
    10861 gagcagaagc ttttaatcta tatactggat tagaaatgcc tgtagaagaa gttaatgaat
    10921 tatgctttaa atagataaga gtaatatctt aaacaatgaa aaaacatacg tatttcaata
    10981 tttttaataa taaattacaa attgtcatag tgggttttgc ctcatagtcc agttaaattt
    11041 gaagttttaa ctgactatac tttaaatcca ttatggggat gagtttattg tacacaaaaa
    11101 tgttaataaa ttaacaagaa tgggagaaaa attatgaaca ataaaaaata ttatcctact
    11161 gccatagcgt tgtatttttc atatttttta cttggtattg gaatttcaat tttaggacaa
    11221 tacaaaccag aattttcatc aatgtggggg gctaaaacat tatcagatgg aacattggat
    11281 gtaagtattg ttttagctgt tattgctgca ctaggattag ggagacttat atcttatcca
    11341 tttgcaggac ctatttcaga taagtatgga agaaaagtaa gtggtcttat aggaaacttt
    11401 ctacatgcaa ttttctttgt tggaattgta ttttcaccaa atttttatat agcatatgta
    11461 tttgcaataa ttggaggggc agcaaattca ttcttagata cctgtgtaac tccttcttgt
    11521 atggagatat ttgctagttt aggaacaata gcaaatatgt ttactaaatt tacaatagct
    11581 ttagcacaat ttttgcttcc ttttataatt ggatttgttg cagcaaattc aatatcattt
    11641 aaagttattt ttatagtaac agcgattctt attgtagtag atgccttttt gatagctgtg
    11701 ttaccatttc caccagctaa taatgttatt gataacaaag gcaaaacagt taaatcagag
    11761 aagatgaaat ttacaccaac aagtatagct cttgtttgta ttggatttac atgtacatct
    11821 acatttgtat tatggttaaa ttgtaatcag gaattgggta aattgtatgg gatggctgac
    11881 cctacaaaaa ttcaatcctt ttactcaatg ggagtaatat gtgcagtatt aataacttct
    11941 ttacttatta aaaaatatat taaaccaata agaatattag ttatatatcc tataatagct
    12001 ttacttatgc tattagttgt atattttgtt caaactccaa ctatttgtat gttaggggga
    12061 tttgtaatag gttattcagc agcaggaggt gttcttcagt taacaacgtc aactgcaaat
    12121 gaaatgtttc caactaataa aggaaaaata acatctatag ttatgattgc atctagtata
    12181 gcaaactatg ttattttaaa tatagcaggt attattacaa aatcaggggg agttaatgga
    12241 ccaaaatatg ttgtattgtt taatgtggca ataacatttg ttggaatttt acttgcattg
    12301 tttgtaaaca tgagatatga aaaagaaaaa gtgtatgatt atgatgtcta atattagtat
    12361 aatatatgca tagagaagtc taaatctagg atagattttt attaaaagta attaaaatta
    12421 ataaggatat tatttacagt tataagatag ggtatttaca taattaataa taaggatatt
    12481 actagaattt caatttctag taatattttt ttgcgtataa atgtaaactt gatgaaataa
    12541 gtagtataat caatataaat tacatcatgt aaaggaggat aatttggtgg agaatgtttt
    12601 taagcgattg caggaattta atggttatga tggatacaaa gaatcatttg aaatgaatta
    12661 cctatgtata tatgaaagta taccgttaag agaacaagta gagcttgcaa ataatctagt
    12721 agatgaaatt ttaaatatgt ataaaagtga atctaatgaa atttatttat tggagggtag
    12781 taattctaag tctttaattt gttatttcga aatttttatg aaaaagataa atactttggt
    12841 aaaagaaatg attattgatg agaaatggtt atataaatta actaaagaat taatatataa
    12901 gagtaaaaaa gttgagtatg taaaactagg gttagtttta agtgagaaat atctaaatgt
    12961 agaaaactta agagaagttg tagatacatt ttctaaatct ggagaatacg tattttatct
    13021 gagtaatact attaaaaaac ttgaatttta caatacatat ttatttaatt tatctaaaaa
    13081 ggctacaggt tcaataaaag tatttgctat tgttaatatg gaaaatttag attctaaaat
    13141 aaattcctat ctaattgaag atggttataa ggatacaaag tacgaacgat tattgatgaa
    13201 ctatattatt tcaattgtag atttgaatga atatcttgaa aaacgagatt tagataaaga
    13261 aaagataaat aatttagcac gtcttatatg caattacttg ctaagtgtag aatttaaata
    13321 tataggtaat aaattagaat tagtgaatcg atttttacct actgtagtaa attatgggac
    13381 taactttgaa agtttatatt caattttcct aatagctata aacgtcttaa aagatgaaaa
    13441 tatagagtgc aataagattg aatttgaaaa agaaataaat ggtatcttgc tcagtgaaaa
    13501 atggaaaaat atatattttg aagcattaag agatgctagt ggaaaaacag aagatattat
    13561 aaaaatgtca gaaatttatg atgtaaactt atcttttgat gacttgttgc catatctaaa
    13621 tagagatatt agagattttg aggtatattg gcatatatct aaaaagggaa cgacaagttc
    13681 tagattaaaa ttattaaatt tttttgaaga gacgtttaaa atagatgatt taattggaaa
    13741 aatgaaagat atagaaaaag ataaattgac tcaagaatat tatgatgata tgttattttt
    13801 tattgtgctt aaaggaagta aatcactata tcctgaagga aagaatatat ctttaaaagg
    13861 aatatttgga aatataaatg aagtaagaaa agaatccata aatatcttaa aaagatacag
    13921 agagaaacta tctcttgaag aattaaaaat tgttaaagaa gcgtatgaaa aagaaaaaaa
    13981 tgttatattg aaggatgaat taagaagagt attatatgaa agtaataatt taaaaaaaga
    14041 atttgtaaat attgaaaaaa taaaagtaga tgaacatggt aaggatatct atttgacttc
    14101 aatagctgtt gctggttcaa ggtttagaaa tagagaatat cttgaaaaag aattggagaa
    14161 aagtaagata tactatttaa ctagagagaa agataatcta tatgatgaaa aagctattaa
    14221 aatagttggt gaaactggat atgtgatagg ctatgtccca agaaaagaaa actatatttt
    14281 gagcaattta ttagatggag gaaaactgct gtattgtaga gtaactgaat ataatttata
    14341 tgaagattgt atctacgcaa atgtgtactt gagttataaa gatgtaattg aaacagttga
    14401 aaactcttta aagatggttt tagataagag tagaataaaa cttataaact aagacgttta
    14461 tatattgctt agatgatgta atcctaactg attggttatt ttttctggaa ttatggtgta
    14521 ttttatgagc aatgctaatg aatataaaat aaaaaaacaa ttgttttgta tatatgatgt
    14581 atataaatat atttatatat tcatatttac aaaacaattg aaatttatgt tattttataa
    14641 ttttaaattt agatggaatt ttaacttctg ctttatcttt tattttagaa gcttgttttt
    14701 taactttatt taataattca gattcatcta caactaatac ttttctttcc ttcatcaacc
    14761 atttaccatc acaaatagta gagtctacat tttcactagt catagaataa actatatttc
    14821 caattggatt atgcattggt aaacaatgaa ttgtatctgg atttagaata atcatatcag
    14881 ctttttttcc tacttcaaga ctaccaattt ctttttctag taaagcacat tttgctccat
    14941 ttatagtagc catttctaag acttcttcag ctggtacgac agttgggttt aaagttcttc
    15001 ctttatgtat tagtgatgta agatacatgt ccctcataag gtccattcta ttatttgaag
    15061 gagctccatc agtaccaatt gaaacaggta ttcctttttc taacatttca ggaattgatg
    15121 caaaccctag aaccactttc atagctgaac ctgggtcatg agaaactttt acatcatgta
    15181 atctaaataa atcaatttcc ttatgagtaa gccaaactgt atgagcagct aaaagatttg
    15241 gtcctaatac acctagttta tcaaggtatt caactgttcc aactccattg ttttgcttta
    15301 caaatgaaat ttcatcagct atttcagcaa tatgcatatg tatacctgta ttgtatttat
    15361 cagctagttc tttagttcta acaagtaatt cttcagttgt attaaaaata gttcttaatg
    15421 caaaccatat tttaattctc tcattagatg tattattaaa cttatggaat aagtctaatt
    15481 gagtgttgat ttcctcatca gtagtttttt gccatgcctc aggaagacca tctcctgtat
    15541 ccataactga tttagctaaa caggctctaa gtccagtttt atcaacagct tcaaccatag
    15601 catctacata ttgaccacca gattctaaaa atgtagtgac accagatttt atcaattcaa
    15661 cacaacatgc agtagatgaa atcaaggaat cttcataatt gaagctactt tcgtatggcc
    15721 aaattctttc tctaagccaa gttaaaagta caacatcatc tgcaagacca cgtcctaatt
    15781 gttgagataa gtgaacatga gtattgacta gccctggcat tagtattttt cctttagcat
    15841 catatatctc tgcatcttta tctacaatat taatatcaaa cttaccaacc tcttttataa
    15901 tattattttc aataagtatg cttccatttt catatacctc tctatctgca ttcatagtaa
    15961 ttagatatgc attttttata agtaattgtg acataaacac cactcctttt ctatatattt
    16021 ttatatctat atattatgat aataaatttt ctatacaaag ttaatagcaa aatcaattta
    16081 catataaagt gattttgcta tttataaatt taaaataaat aagattttat tctatataat
    16141 ttaaaatatt gacctagaac tatttaaaaa tattaactta gaactaagtt tacaactgca
    16201 agtaaaccta taatccaaag tgtaatactg attttatcag tttctttagc tactacatgc
    16261 attattatat atgaaataaa tccaaagcat aaaccaatac ttatactata agttaaaggc
    16321 atcaataaaa ctgtaacaaa agcaggtaca gctattttgc tatcagcaaa atcaatctgt
    16381 tttatgcttt caaacatgaa tacaccaacc attacaagtg caggagctgc agcgtaagta
    16441 ggaactatac caagtaatgg tgtaaatata agtgtagcta agaataatgc tgctgttact
    16501 attgaagcaa gtcctgttct tgcacctgct gcaataccag aagcagattc tccaaatgtt
    16561 gtaacagtac tagttcctaa gaatgagcct attaaagtag aagaaacatc tgcatataac
    16621 attcttccta aatcttttat ttcaccattc tcatcttcaa gacccatttg tttacaacaa
    16681 gtaataagaa atgctaatga atcaaacatg tctataaaca tgaaagagaa tataggtcca
    16741 attaatgaga atttcaatgc acctacaata tctaatttaa aagcaactgg tgctatacta
    16801 ggaggtgtcg agattattgc tgttggcaac tccacaaatc caaatataac agatattatt
    16861 gttgcaacta ctatacttat caatattcca cctttaactt ttttatattc tagtattgcc
    16921 attaagataa gagcaaaaag agctattaaa actgttggtg taaaatctgc aagtgaaact
    16981 atagtatctg gattagctat tataacaccc atatttttta aaccaacaaa agcaaggaaa
    17041 agacctatac ctgctgaaga tgctactgcc aattctctag gaattgcgtc tgcaattgtc
    17101 tttcgaagac ctgttactgc gagtgtaaag aagacacatc cagatagaaa aactacccca
    17161 agacctgttt gccatgggat tcctgcacct ataaccaaag agtatgtgaa gaatgcattt
    17221 aatcccatac caggagctag agcaaatggt gcatttccta aaatcgcaaa tataagtgta
    17281 ccaatggctg tagctagtat ggtaactgtt ataagggcgg ctttatccat tccagcttca
    17341 gataatattg ctggatttac gaaaactatg tatgccattg ttaaaaatgt agtaactcca
    17401 cctattacct ctgttttaag gctagagcca cgttcgctaa atttaaacat tttgtctaaa
    17461 aagcttaatg atgaattatt tccacctggt tgtacattgt tagtgtttgg taccattttt
    17521 acctccaaaa aactttaaga tataaacaaa aaaatatata ttcatataga tagaatagaa
    17581 catatattca gagttgacat tctattcata gaaaacccat ttaaggtgag tttgtagaaa
    17641 ctattagacc gtattactaa tattatatga acattttcta tttgtttttc tgttgtatta
    17701 tatcgtaata ttgtattatt tacaataaat ataataaaat tattcgtttt tttgtcgatt
    17761 ttataaaaaa ttaaggttat ttattgaaaa aaa
//
LOCUS       DAELNY010000070        17586 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.070, whole genome shotgun sequence.
ACCESSION   DAELNY010000070 DAELNY010000000
VERSION     DAELNY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.070"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            123..2186
                     /locus_tag="KPE55_001991"
     CDS             123..2186
                     /locus_tag="KPE55_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0493059.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2183..4048
                     /locus_tag="KPE55_001992"
     CDS             2183..4048
                     /locus_tag="KPE55_001992"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0493060.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4172..4986
                     /locus_tag="KPE55_001993"
                     /pseudo
     CDS             4172..4986
                     /locus_tag="KPE55_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5011..5499
                     /locus_tag="KPE55_001994"
     CDS             5011..5499
                     /locus_tag="KPE55_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0493061.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5612..5773
                     /locus_tag="KPE55_001995"
     CDS             5612..5773
                     /locus_tag="KPE55_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493062.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            6013..6240
                     /locus_tag="KPE55_001996"
     CDS             6013..6240
                     /locus_tag="KPE55_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493063.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6237..9176
                     /locus_tag="KPE55_001997"
     CDS             6237..9176
                     /locus_tag="KPE55_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0493064.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9176..9850
                     /locus_tag="KPE55_001998"
     CDS             9176..9850
                     /locus_tag="KPE55_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493065.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            10011..10427
                     /locus_tag="KPE55_001999"
                     /pseudo
     CDS             10011..10427
                     /locus_tag="KPE55_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10387..12834
                     /locus_tag="KPE55_002000"
                     /pseudo
     CDS             10387..12834
                     /locus_tag="KPE55_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12974..13396
                     /locus_tag="KPE55_002001"
     CDS             12974..13396
                     /locus_tag="KPE55_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493066.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13934..14968
                     /locus_tag="KPE55_002002"
     CDS             13934..14968
                     /locus_tag="KPE55_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG0493067.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDERQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15055..15258
                     /locus_tag="KPE55_002003"
     CDS             15055..15258
                     /locus_tag="KPE55_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG0493068.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15429..16589
                     /locus_tag="KPE55_002004"
     CDS             15429..16589
                     /locus_tag="KPE55_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG0493069.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16848..16988
                     /locus_tag="KPE55_002005"
     CDS             16848..16988
                     /locus_tag="KPE55_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493070.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7195 a         1704 c         3024 g         5663 t
ORIGIN      
        1 ttatttttta ttcgtataat aatagaaata ttttagcata agatgtataa taaaggaaaa
       61 aatacagaat taaaaaacat aagatagata agtggactta ggggagagaa attattatgc
      121 aattgacaaa aataattctt agtaactata ggtgttttgg agaagaacct acaactattg
      181 aaattaataa tttaactgga tttataggtc ataatagcag tggtaaaact gctttattaa
      241 gtgcacttat gaaacttttt ggagaaaagt ctagtgatag aattattgaa agatcagatt
      301 tccatatacc tgaaagtatt gatccagata gtatttctaa gaatgaatta tatattgaag
      361 cacaatttga gtttcctgaa ttggagagag gtaaagacag catatctata ccagtatttt
      421 ttaaaaatat ggttgttagt gaaccaggtg ggaaacctta tttaagaatt agacttgaag
      481 caaattggga gaaaagctca aatcctgaag gaagtattga ttcaaatgta tactatataa
      541 catctacaac agaggagata aatcctgaag ataagcgtaa tatcaaaaga tatgagcttg
      601 atcaaataaa gatgatttat gtacctgcca ttaggagacc agatgaacag ttaaaaaatg
      661 tctcaggaac aataatttca agattattaa atggtataag ttggtcagaa caaacaaagg
      721 caagaattaa aacgaaagca agtgaaacgg aggaaatttt ctttgaagaa aaaggggttg
      781 aatcattaaa gaaatcactc gaaactcaat ggaatagtta tcataaggat attagatatt
      841 ctaatccaaa gataaacttc aatagtactg atttagagac aattttaaaa aaagttgaag
      901 taactttttc acctacaggg acacatcgag attatcgggt tgaagagctt ggagatgggt
      961 tacgttcttt attttatctt tcacttgtaa atacattttt agaaattgaa gaagaaattc
     1021 taaaatctat taatgatggt aaagaagaag atatatttac attaataccg cctgtattaa
     1081 caattgttgc ggttgaagaa ccagaaaatc atatttcacc tcatctttta ggaagggtag
     1141 ttgaaaaatt aaattttatt tctacaaaag aaaattcaca gacaatattg acatcacatt
     1201 caccagcaat agtaaaaaga attgaaccaa cagacataag gtattttagg atttgtaagg
     1261 aatatgaatg tacaaaagtg aagtcaatta tattaccaaa tgaagaaaag gaagcagaac
     1321 aatttaaatt tgtaaaggaa tcagtattag catatcctga actttatttt gcaaaacttg
     1381 ttgtattggg cgaaggtgat agtgaagaga tcattattaa gcagatgatt gaattaaaag
     1441 cagaatctgt agattcatca gaaatttcaa tagtaccatt aggtggtagg catgttaatc
     1501 atttttggcg tttattaaat gatttagaaa ttccatttat tactttgtta gatttagata
     1561 gagaacgaga tggaggtggt tggggacgta taaagtatgc aatacaacaa ttaataaata
     1621 ttggtcatga taagaatgta ttactttata catgtgaagg aatttcacaa gataatgaat
     1681 ttaaagagat gcatacttgg gatgtaactg atactgaaaa tatggctaaa tggattgaaa
     1741 tgctaaaagg ttatgatgtt tatttttcag ctccgttaga tatagatttt gcaatgttac
     1801 aaagttttaa agataaatat atttcagcat taacaagtgc ggaaggtcct aggataaaag
     1861 atcatggaaa aatacaagat attgtgatca atgaagatgc tgaagatgaa tcattgaaag
     1921 ctgcttataa agatagaata gatagtgata ttaaatcaac cttaaaacaa gaaggtggtg
     1981 atggatctac ttatactgag ggagagaaag aactaatgat atggtattca tatttctttt
     2041 taggaagagg taaaccaact actcatttat ctttattttc taataatact gatatagatt
     2101 ttgaaagttt tccatactgt ctaaaggagt ttgtaaatag tattattgaa aagatgatag
     2161 atgattcagg gggtggaaga aaatgaagag gaattgggtt agtaaagaag attggagtcc
     2221 agcggctgga ttcagactag aagttgaagc gatgaataca gttattaatg ataagaatac
     2281 acttgttgta gctggtcctg gcgctggaaa aacagagtta ttagctcaga gagcctgctt
     2341 tttactagag actaatacct gtagatatcc taaaaaaatc cttgctatta gttttaaaaa
     2401 ggatgctgct gaaaatttaa aagagcgttt agaattgaga tgtgggaaag aattagcttc
     2461 aagatttgaa tcaaagacct atgatgcttt tgcaaaagaa atcgtagata gatttagaaa
     2521 tagtttagat gaagcatata gacctttaaa taattacaat atagctgaaa atcgtgatgt
     2581 tagaagagca tttgagatag caggattaaa tttcagagga aatcaaactg aatttaataa
     2641 agtatatgga aataaactat cgataaataa attgccttta catacagatt ttgtagataa
     2701 gatttttata gatgcttgga atgtactact taaaggcaac aaagatacta attttgtaag
     2761 ttcacttact tttggtatga tatcacgttt agccgaatat ttgataagaa ccaataggta
     2821 tattaagaaa gcaattgaaa tgacttatag tcatgtgttt ttagatgagt ttcaagatac
     2881 taaatctatt caatatgatc ttgttaaagc ttgttttttt aaaacagaaa caataattac
     2941 tgctgtaggg gatggcaaac aacgaattat gttatgggct ggtgctagaa agtcaatatt
     3001 tgaagatttt caaaatgatt ttttaagtca aaaaagaaca ttaattatga atcatcgttc
     3061 agcgccaaga cttgtagaga ttcaaaaagc aatgtataac agtttgaatg aagaggcaat
     3121 tgatttaaaa actaatgaaa agtggaatta tgaagatgga gaagtgtatt taagactttt
     3181 tgaggattat ataaaagaag ctgatatact tagtgaggaa atagtcgaat tggtggaaac
     3241 aggtatagaa attaatgaaa tatgtatatt agtaaaacag agcgtagata tttattcaaa
     3301 agagattata aataaacttt ctgaacgtaa tattaaggcg cgtaatgaag caatgtatca
     3361 ggagatatta aaagaagata taataaagac actggtttcg ctttttaggt tgtcgttaag
     3421 tgatagaaat cccgatgatt gggattatat ttacgatatt ctaggtgaat tatatggaat
     3481 agacgaaggg tataacccta aaatattaaa tgatttcatt accaaagtag aattaatgat
     3541 taatgatctc aaggtaaatt taaatattac tgatgaaaaa caattttcag atttagttga
     3601 ttctaaggtt gaatatattt catatgaaaa atttgcatca aagtttcaaa aatataaatc
     3661 taaagattat tttgatgatt tggtagataa atttaaaaag ctggtatgga ttgaatataa
     3721 tgaaactaaa gattggatta aagcgattga taactttgaa ggtaaaaatt caattccaat
     3781 aatgacaatt cataaaagta aaggacttga atatgatact atcttttttg ttgggttaga
     3841 agattcagca ttttggaatt ttaaaaatca accagatgaa gatagatgtg cattttttgt
     3901 tgcattatca agagctaaaa gaagaattga ttttacattt agcttagata gaccagcagg
     3961 attttcgaat tcacagaagc attattgtat taatgaattc tacgaattgt ttgataatac
     4021 tgagattgta aaaaagatag agtattgatt aagatgataa aaactttagt atattatatt
     4081 ttaagatatt agttatataa gtagacattg aatttatgta tttgatgtct attttttata
     4141 taaaaatata ttaaagaaag gatgaatttg catgaagaaa aaaacaaaaa tatctaaaaa
     4201 gattgtaagt tttattatgg tttttataac tatagttact actatactta catcatcagt
     4261 ttcagcttat gctagcgagt tggatatgag tgaacagaca ggctttaatt atttaggtat
     4321 tagtccaatt acaggaaaaa aattaatcat aacatttatc gtatgaagat ggataataaa
     4381 attgtatttt gtgtagagcc aggaattaaa acttcaagtg gaggaggata tatatcggag
     4441 gagttaggag ataaatttat atctacaaca cttccaaatt atcataaaag aaaagctgaa
     4501 atacttgaga aggtaaatag acataatata ttaccttcat ggaataatga aaaagttgca
     4561 gttaaaagtg gagaaacaat tgagttagaa gataagaatg gtattattag agaaatgcaa
     4621 ttagaatcaa ataatactgg aacagatata ttacttgaag aaaatacttt gaaaattaca
     4681 gcaaataaaa attctaattc aggagttatt agttatataa agattcctaa aaatgaggtt
     4741 ggaatatcaa tagtttatcg taagcataac tatcaaagct tatcagaatt tcatatggaa
     4801 agttcgatga gtgcaaattt aaatataaat gtaatcaagt taggaattgt taaagtaaaa
     4861 aagattgatg aaggcacagg aaaggaatta gcaaatgcaa aacttagatt tgagtatgat
     4921 ggtgatagta aagaaattat aacgggttct aatgaaattg ctagtataga agatattatt
     4981 ggttgattaa aaagtgaagt atacttttgt atctatgaaa aagactataa gcaatatata
     5041 aaattagata tacctattga tgaagcaaaa ataaaaaata gatttgaaat aaggttaaaa
     5101 aatgaaagag catatcatgc agcagttgat ttgttagcaa atagagatga agaaaagaca
     5161 gcattttcaa ttattaatcg ttatatacgt tttgttgata aagatgatga aaaaattaga
     5221 agtgaatgga aaactaatga tcagtggagt tattttattg gtgaaggtag agcgaagtta
     5281 aaactaacaa caaatccaga accatataat attcaaaaaa ctttaaattg gttatcaaga
     5341 caagttgcac caacgttaaa aacaattaaa aggttagata gtttgaacaa ctcaacatta
     5401 attgagggct taataaggta tgcaagacta acagataaac ataaaaaaat aattgaacaa
     5461 aattcagtaa atgttgaaga cattatttat gatgaataaa aaaatgtaat tagtattgca
     5521 gattttaata taaaagaagg gagatatacg aatgaatttt ggtcaaaatt tatataatta
     5581 gtttttatca aatgcacaat cattggtatt aatggctata gctataattg gtgtatattt
     5641 aggatttaag agagagtttt caaaacttat aggattttta gtagttgcat taattgcagt
     5701 agggcttgtg tttaatacag caggagttaa agatatatta ttggagctat ttaataaaat
     5761 aataggtgtg taaggattaa aagagaatag aaaattatga gtatatgatg aagggttcta
     5821 ataagtttta ttaaaatata tattgaaaga caagtagagc tatttcgagt tattacatgt
     5881 aatgctaata tatataactg cattttggaa attaaataca taattagaaa tagcaaattt
     5941 tataggtgac ttattaatat gtttacttat tgtatactat ttgatatatt taatatgagg
     6001 tgattcttga aaatggacat agtgaaagtt tttgctaaca atgtgagaaa atatagaaat
     6061 aaaaaaggtc tatcacaaga agcttttgct gaaaaagctg gattgcatag aacttatatt
     6121 tgtgctattg aaagaaaaaa gagaagtatt gctttagata atgtgcagaa aattgctgat
     6181 gcgcttgaga ttgagactta cttactattt atagaaggtg aggagaaaga atgtctatga
     6241 aaaaattacc aatagaaaag cttatagaaa atttaggttt ttcagggtca aagaatcttg
     6301 tgaatataga tatgcttaca aaattagata tatctaaaca tgatgtaaaa actttaaaag
     6361 ttataaatcc ttatgcaatt tatttagtaa atagtaagcc atttatatta tttatagagt
     6421 cttcttttga agaaaaaaaa ttacaacaaa ttagtaagct tatatggaat gctcaaatac
     6481 caattgcttt tatatgcgat tctaattctg ttaaaatatt taatggaaaa gctttggatt
     6541 taaagtcata tttaattgag gaaatatata atcaatctat tgaggatttt tcggaagaat
     6601 ctgagttttc ttatttgaaa atatctgatc cattattttg ggatcaatac actaaacaat
     6661 ataccaaaac gcatttaaat gattatttgc tagataatat tacttcattg acaaataaat
     6721 taaagaaaaa atataaggtg aaatttgcaa cgaagcttgt tctaagatta atatttatta
     6781 gatatttaat cgatagaggt gtcgatatag cttataaaaa ttttaatcat gatattaaag
     6841 cttcacaaaa agaactgcta aagattacta aaaacaaagt tgaattatat gaactgttta
     6901 aatacttaaa agaaaagttt aatggtaatt tatttgagtt aaatgacgaa attaataata
     6961 cttgtttaac agatgaagtg tttattttac tatctgattt tttatcaggt aaaataagta
     7021 ttgacaatgg acaaatgtct ttattttcaa tgtatgactt taatatcata ccagttgaac
     7081 taattagtaa tatatacgaa attcttttag gaaaagaaat tcgtaataaa gataacgcat
     7141 tctatactcc gaattactta gcagaatata ttttagataa aacaacactt gaatttctga
     7201 aaaataatag gcaatacaaa attctagatc cttcttgtgg atcaggagta tttttagtaa
     7261 atagttttag acgtattatt gatatgaatt tgggtgataa acttttttgt gaagatgata
     7321 aacttttaaa agaactttta ttatctaata tttatggaat tgatatcaat gaagatgcta
     7381 tcgatgttac tattttctcg ttatatctaa ctattttaga ttacaaagac cctaagatat
     7441 tatcagaatt taaactgcct aatttaaaag ggtgtaattt aatagcgagt gatttttttg
     7501 atgataataa attggagagt ttaaagaaaa ttaactttga ttttatcatt ggaaatccgc
     7561 cttggggaag tgtctcagat ggattacatt tagattattg caataaaaat ggctataatg
     7621 atatgcaaca aaacaaagaa atcagtagaa gttttatttt tagagcaaaa gattttagct
     7681 ctagaaatac agtatgttct tttgtagtgc attctaaaat actatatact caaaaaaagc
     7741 catcaaaaaa attccgtgaa tttttgctta aaaaaagtca aatatttagt gtagttgaaa
     7801 tgtcatctgt aagaaaatta gtgtttaaaa atgcagacgc tcctgcagca gttatatcat
     7861 ttaagtataa cgaaagtgaa gaaaatttaa ataatagaat catctataca tctttaaaac
     7921 caaatgtatt ttttaaatta tttaacatta ttgtaattga aaaatatgat gttaaatata
     7981 tttcacaaaa actattattg gagaatgatt gggcttggaa aactattgtg tatggattca
     8041 gtaaagatat agatataatt atgaatatca aaaaaaaata tcaaactgta aatgaaatta
     8101 ttttgaaaaa cagttttgtt catggaacag ggatacaaat caatggtaat gaaagccatg
     8161 atgcgacaca tttaaaagga aagtggtatg ttgattcaaa gaaaggaata gattcattct
     8221 atgttgatat aaataaaggg aaagaactta ctcaagataa aatgagaaga cctaaggcta
     8281 atcagcaaga aatatttatt cctccatatg tattattaaa aaagggagtt gacacgaaaa
     8341 catatagata tagagcagca ttttcaaatg atgcatttgt atattcagat gcagttacag
     8401 gaatatgtgg aaaaaaagat aatttaacta cactttattg cttgactggt atgcttaact
     8461 cttcttttta ctcatactta aatataatgc taggtacatc aattggaata gaaagagaac
     8521 aaggattttc aacggaaatt tttacgtatc cagcaataga aagtgatgaa attgctaatc
     8581 tggtagtaaa aatacaagaa gcgactaaat tagaaaaaac aactatgttc ttgaatgaaa
     8641 aaaatagttg taagtatatc aaacaattag ataagttaat tttgaagtct tttaatttaa
     8701 atgatgataa atttattgac tatgcaataa atattcaaat tcctgagctt acaaattcta
     8761 agtcaactaa catatataga aaaataacta aggaagattt aattaattat agtgaatgtt
     8821 tcaaagaaca gttttcatcg atatatcgta gggttgggaa aagtatttca atttcggtat
     8881 atccaaaaat attaaatcgt tttacagtat ttgaactaga agtaacagat gaaaaaacag
     8941 atagagagat tatggttaaa gcagaagctg ataataatat ggagttgttt acgaaaataa
     9001 ttagatatga ttataatgat gtttttcatc aaattagaga tattattcat tttggagaaa
     9061 attcatttta tatcataaag cctaattgct ataaatattg gcatcctgct atcgcagaaa
     9121 tggatcttgc tgatgttttg gatcagattt tatctgatac agggggtgaa gaataatggg
     9181 aggatttaaa aattcttcac taaactttta tttaaagcaa gggctttatg aaataattat
     9241 agatggatta atagactgtt gtttacttat gagcaaatca tgtattgaac aatctagaaa
     9301 aatattaaat cacgaagaaa agataagaac gcatttagtt gaaaattatt tagaaaatga
     9361 tattgaaaga agaaaaacat ctttatctaa acaacatcta agatttgaag tagaagcaca
     9421 agaaaattat gacaacaaaa atgaatctta tgagggtagg gttgatataa aagttatatc
     9481 taaagacact ttagcggata gaagggctta ttatatcatt gaatgtaaaa ggatagatgg
     9541 taaggaaact ttaaataaaa aatatataac cgaaggtgta caacgattta ttactgagaa
     9601 atattcttcg cattatggca aaaatataat gcttggattt atagtaaaag atataaatat
     9661 ccagagcaat atacaaaaaa ttaattcact gaatcagaac atattatata catatataga
     9721 tacagatatg ttgacagtta gaagtgattc tgaaaataat tatgctgttt gtgaaagtaa
     9781 atataaagta aaatctaaac tactattgtt aaatcatatt ttttataact tttcgagtat
     9841 aataagataa tcttatgaat ataaaatatt actatgttaa aattatagtt tggttttgat
     9901 aatattataa aaataagttt aagaataaaa tataaaggaa aaaaggggcg tagcactatt
     9961 ttttagtgtg acagcccctt taattttaaa atcggtaagg aggagtgtat ttgaaaaaaa
    10021 ttaagtcata tacagggatt ttgaatgtgg agaaggttat atatgcaata aatgattttc
    10081 agcttccatt tccagttact tttacgcaga tgacatggtt tgtattaaca ctttttataa
    10141 ttatattatt tggtaagtta cctccattat ttatgataga aggagcattt ttaaagtatc
    10201 ttggcatacc aatttttata acttagttta tgtctcaaaa gacttttgat ggaaagaaac
    10261 catatggatt tttaaagtca ataatactat attcattaag acctaaaata acatacgcag
    10321 gaaaaaacat taaatttaaa aataaaatta ttgatgaaaa tataactata gtaaggagtg
    10381 aattatatgt tcccgataaa atatatagag aacaatcttg tatttaataa ggaaggagaa
    10441 tgttttgcat attatgaatt aataccatat aattattcat tcttataagc tgaacaaaaa
    10501 tttattgtac atgataattt taggcaatta atatcacaaa gtagaaatgg taaaattcat
    10561 gcacttcaaa tagccactga aagttctata agagttacac aagaaaggtt aaagaaaaat
    10621 attacaggaa aattaaaaga aattgcttat gaaaaaatag acgagcaaac agaggcactt
    10681 gtttctatga ttggcgataa tcaaattgat tatcgttttt ttattgggtt taagctttta
    10741 gttacagaga aagaaatgaa tataaagagt ataaaaaagt caatattagc taatctatca
    10801 gattttatat atgatgttaa taataaattg atggatgact ttgtcagtat aaataatgat
    10861 gaaattatga gatttttaaa aatggaaaag ttattagaaa ataaaatctc aagaagattt
    10921 aaagttagaa ggttagataa aaatgatttt ggatatttaa ttgaacataa ttatggaaag
    10981 aaaggcacag tttttgaaga ttatcaatac catttaccta caaagaaact taaaagagaa
    11041 acattagtaa agaaatacaa tcttataaag ccaactagat gtcttataga agagaagcaa
    11101 agatacttaa aaattataca tgaggattca gaaagttttg tagcttattt tactattgaa
    11161 attattattg gagaaatgga ttttccatct agtgagatat tctattatca gcaacaacag
    11221 tttgcatttc ctattgatac tagtatgaat gtggaaattg taacaaataa aaaggcttta
    11281 actacagtta gaaataagaa aaaagaatta aaagatttag acaatcatgc ttggcagtca
    11341 gggaatgaaa cagtaacaaa tgtagtagaa gcattagagg ctgtaaatga gcttgtaaat
    11401 aatttaaatc aaacaaaaga atcaatgtat aagttaagct atgtaattag ggtatcagca
    11461 gatagcattg atgaattaaa acgccgttgt gatgaagtta aagattttta tgacgattta
    11521 aatataaaat tagtacgcct aatgggagat atgataggat ttcacaatga atttatacct
    11581 tcaagcaaaa ggtatataaa tgattatatt caatatgtaa ctagtgattt tctagcagga
    11641 ttagggtttg gagcaactca atagcttgga gagaacgaag gaatatatat tggatatagt
    11701 ttagatacag gaagaaatgt ctatcttcaa ccatctcttg caagtcaagg agtaaaagaa
    11761 tctgtaacta atgcgttggt agcagcattc tttggatttt taggaggtgg gaaatcattt
    11821 gctaataact tacttgttta ctattcagta ttgtttggag ggcaagctgt tttggtggat
    11881 ccaaaagctg aacgtggcaa atggaaagaa aatttaccag atatatatca tgaagtaaat
    11941 attgttaatt taacaagtga tatagaaaat aagggaatgc tagatcctta tgtaattatg
    12001 aaagaagtaa aagatagtgg aagtctagct attgatatat taacttttct tacaggaata
    12061 agtagtcatg atggagagca gtttcctata cttagaaaag caatcataaa tgttactcaa
    12121 gacaaatgca gaggactttt gaaggtaata gatgaattaa ggaaagataa ttctcaaata
    12181 tcaaataata ttgcagatca tattgaaagt tttacagatt atgattttgc acaattactt
    12241 ttctcagatg gaaatattaa aggctcaatt aatctagaaa aacaaatgaa tataatacaa
    12301 gttgcagatc ttgttttacc gaataaagat actttatttg aagaatatac aacaatggaa
    12361 agtctttcag tagctatgtt gatagtaatt agtacttttg cattagattt tatccataca
    12421 gatagaagta cttttaaaat agtagattta gatgaagctt ggacatttct taatgtagca
    12481 caaggaaaaa ctttatctaa taaattagtt agagcaggat gtgctatgaa tgcaggagtg
    12541 tattttgtaa ctcaaaatgc aggtgatgta tcagatgaaa atttaaaaaa caatattgga
    12601 ttaaaatttg cattcagaag cacagatatc aatgaaatta agagaatact tgaatttttt
    12661 ggagttgata aggaagatga gaataatcaa aaaaggttaa gagagttaga gaatggacag
    12721 tgtttaatac aagatttata tggacgagtt ggggtaatac aaatacatct agtttttcaa
    12781 gaactcctta ctgcatttga tacaagacca cctataagaa aagaggtgga ttaatgaaat
    12841 cgaaagtatt tagaaattta agctggataa ctattttaat agtagtactt ttactatttt
    12901 ttatttatat aaatttatag atttagagaa acaaatagat acattagata agaaaagaaa
    12961 aaaactcata gatatgagat tagaagatat aattgataaa gaatcatatg aagaaaagta
    13021 tgatgattta acagttgaaa taaagaaact tagtaaagag aaaaaaactc ttaaagcatc
    13081 attaataaat gaaaagagta taaaagcaag acttagagaa tttagaaaga tacttgaaag
    13141 aaatgaattg ttagaaaaat ttgatagata tgtatttgca agtatagtag ataaggttat
    13201 agttggagtt attaatgaag atggtatagt tgatccttat aaaataactt ttgtttataa
    13261 aactagcttt gatgataaac aagatggaca taaatttata aataagagtc cgttagaaac
    13321 gtattcaaaa gataacaacg ataataaaaa gaagtgttca tatactaata atgagacatt
    13381 gcgagactgt agttaaactt attagagaat aacaaggttt cagactttta tattgtgtct
    13441 taaaagtaga tttgccacct atttgccacc ataattattt ttgggtggca aatttattct
    13501 acaatgattt caaaaatatt taccgtctgg tttctcatgt tgttagttac atgtaaatat
    13561 gtatccatag ttgttgaagt tttagcatgt ccgagtatat gctggatatc ttttatatta
    13621 gtaccatttt ctaataacat agtatcaggg tttttaaaat aaagtgtgta ttcttctaaa
    13681 gtagtgataa ttaaaatatc aaatatcact atacaaagga aagtgcacgc tttttatgtt
    13741 taacaaaaac gaaatattaa aaaacttatt gaagaatatg atgtcaaaac aactacagat
    13801 attcaagata tgctaaaaga tttatttaca aatacaattc atcaaatatt tacattgtcg
    13861 aattttagcg gtttaaacat aatatattga ctgatataat aaaatcatag aatattttag
    13921 ggggaaatag attatgttta agaaaaagtt aatagctgtt gcaatgagta taactatgat
    13981 tagtgtaggg agcttttcgt atgctcattc aggaagaact gattcatcag gaggtcatcg
    14041 tgataataaa aataaatctg gattaggaag ttatcactat cattgtggtg gttatccagc
    14101 tcatttacat aataatggtg gttgtcctta tactggagga ggaagcagtt ctggaacaac
    14161 aacttcggtt aataatgaag aaaagcaaaa gaggtctgtt ggagaaaaag gatataatca
    14221 agggtatgaa gatgggtata aaggaaatta tagttcatca aattatagtg gagattatag
    14281 tgatacatat gaatcaaagt actcagaagg atatgaaaaa ggtaaggcta aattagaaga
    14341 ggaagaaaag gtggctaaag agactggata taatttaggg cttacaggtg ctaaatctaa
    14401 taatacatat gaaaaagaag ttttaaaaaa tgcttatgat accggatatt caactggata
    14461 caatgaatat aagactaaaa agatagaaga atataaagct aaagggatag aggatagcaa
    14521 taaagataag gaaaaaatga catttgaaga aaacatagat tctgaattta tagatgctta
    14581 taataatgct tatgacgaaa gacaagaaca attaaaaaat gattacacta ctcaaggatt
    14641 tgagagtgct atcaaaggag aaagatttga cacatcaact ataggtaatg ttaaatatgc
    14701 taattggttt aaagaaggat atgataatgg taaggttaag ttacctaaag caaaagaaag
    14761 tgtatataat caaggttaca atgaagaaga ttttagtata ccagatgaaa tgaagtctat
    14821 tgaaactaag ttaaaaggtg tatatgatga aggtttagaa aaaagagaag aagagaaaag
    14881 tagaaatgct acttatgggg ttggagctgg aacagcagct gtagttgctg gagtagtata
    14941 taagaagaaa aaacgtaaaa agatatagat gctagatata ccaatgatga caagtgtata
    15001 acaaatataa ttttaagtat aaatatttaa aatatagaag ggaaggttat atctatggga
    15061 tttaacttta ggaaaagcat aaatctagga ggaggactca aattaaattt aggtaagaag
    15121 agtgtaggta taagtgctgg tgttaaaggt gctagagtgt cagttaattc aaaaggaaga
    15181 aaatcagcta cattgagtat acctggaact ggaatatcat atacaaaaac tagtacaaag
    15241 aaacgtaaac ctaaataaat atttgatata ctaagaaata gactgttaca aatagtctat
    15301 tttttagtat atagatttaa aaagtgacaa aattttgaaa tataaactaa tttcataaag
    15361 gaaaatatta ttttatgtag aattataata tttaagtaat attataaaat ttataggagg
    15421 atttcattat gaaattaagc aaaatactat tggcaatagg gatgtctgtt tcattattga
    15481 ttgctagttg tccaacatca aacgctctta gttctattaa ttcaataaaa ggtacaaata
    15541 agtatgaaac agcaggttta atagcagata aacaaaatta tgatattgca atacttatta
    15601 attctgaaaa tggattatct gatggattgt cagcaagtgg attgtcagga gctaaaaatg
    15661 ccccaatatt gttaactaag aaagatacaa tcccttcaga aacattaaaa agattagaaa
    15721 aggtaaaaga agtatatcta attggaggaa aaaattcgat aggagcatca gttcagaaaa
    15781 tattaagtga taaaaaaata agcataaata gaataagtgg aactgataga atagatacaa
    15841 gtcataaagt tgcaaaagaa ataggaagag taagtacggt taaaaaagta atattaacta
    15901 atgcttttaa aggcgaacca gatgctatga gtgttgcttc tatagcagtt agagataaat
    15961 ctccaatagt attaactgat ggaaatacta caagttttgc tacagatgga ttagaaactt
    16021 atgcaattgg tggaaaagtc agtatgagtg ataagttaat aaatgaaact aaagcaacaa
    16081 gaataggtgg agtagataga tacgatacaa ataaacaaat aataaataaa ttttataatg
    16141 gctcaaaaga attttacata gcaagtggga ctgatttagt ttattcttta gttggttcta
    16201 caattgcaca aaaaacacct atagtgttag ttggaaatgg aagtaataag agtatattaa
    16261 aaaatgcaag taaaataact gctattggaa atattgatga tgttgttata aaacaatgtt
    16321 taaatataac taataatgtt ggggattcaa atacaggtat tgtaaaagat actaattcaa
    16381 ataatacgaa caaaccaagt aatgagccta gctctaataa tcctggatta actgataaaa
    16441 gtacagtata tgtaactcct aatggaaaat catatcatct aacaaaaaat tgttctacac
    16501 taaaaagaag taaaacaata aataaagtaa cattaaaaca agctaagtca caaggtaaaa
    16561 tagatccttg taatgtttgt gtaaaataat gtattagcca aatatatatt aaaaaagaca
    16621 attaattaag attgtctttt ttattgaaga aatataaatt tatattatgt attattaaaa
    16681 aaaaattatt aattataaaa aataataata aagacgataa gtattaaaat tttatgtttc
    16741 caatatttat gatataatta atatggcaag gaataaatat ttgaaaaagc tatgagtggt
    16801 gtttccatgg aagtttcttc actttcttat gagaggaggt gaaaactatg gaaaatctaa
    16861 tgatgagtat tataactggt gttatagcca gttacattta cgataaatta aagagccact
    16921 ctgagcgacc aactaagagt ggctgggaac ttaatataaa gttccagaaa agtaaatctt
    16981 caaaataatt tgttgaagga aacatcactc aaagttaagt aaaatatgtt tccttgcttt
    17041 tattatatca caaattaaaa atattaacac aaaattttgt gaatatatac ttattattgt
    17101 tctaaaaaaa tatgtaaata attgatttga ttgcatggaa ttattgatgt aatatttaag
    17161 ttaaatatac acaatctcaa ttaaagaaga taagtactat aaattcatat caaaaaaata
    17221 cataaacttt taagttataa ttttataaaa agtcttttag tatcattatt tcaagaggaa
    17281 ttatcatatg aaaaaataac ttcattagaa aagtggataa aaaactggta agaagatgac
    17341 taaagattac atatactatt acttaaatta aaaatttaat ataatttata atgtatacaa
    17401 tttattataa aaaatttaat tttaattttc tatttttgat actataatat ataatcttga
    17461 taatttaatt aaaaatctag attttacata ccaaactggt ttcttatttc gctatcttta
    17521 cttatgaact catatattcc tataaaggtt attttagaaa aagttggtta tttaaacatt
    17581 aatacc
//
LOCUS       DAELNY010000071        17265 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.071, whole genome shotgun sequence.
ACCESSION   DAELNY010000071 DAELNY010000000
VERSION     DAELNY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.071"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            238..1428
                     /locus_tag="KPE55_002006"
     CDS             238..1428
                     /locus_tag="KPE55_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0493071.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1598..2560
                     /locus_tag="KPE55_002007"
     CDS             1598..2560
                     /locus_tag="KPE55_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0493072.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2682..3344)
                     /gene="zmp1"
                     /locus_tag="KPE55_002008"
     CDS             complement(2682..3344)
                     /gene="zmp1"
                     /locus_tag="KPE55_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG0493073.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3444..3529)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3714..6632)
                     /locus_tag="KPE55_002009"
     CDS             complement(3714..6632)
                     /locus_tag="KPE55_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0493074.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIQEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPIVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6750..6835)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6962..8140)
                     /locus_tag="KPE55_002010"
     CDS             complement(6962..8140)
                     /locus_tag="KPE55_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0493075.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8515..8664
                     /locus_tag="KPE55_002011"
     CDS             8515..8664
                     /locus_tag="KPE55_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0493076.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(8765..11539)
                     /locus_tag="KPE55_002012"
     CDS             complement(8765..11539)
                     /locus_tag="KPE55_002012"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0493077.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAVTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11711..12967)
                     /locus_tag="KPE55_002013"
     CDS             complement(11711..12967)
                     /locus_tag="KPE55_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0493078.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(13039..14541)
                     /locus_tag="KPE55_002014"
     CDS             complement(13039..14541)
                     /locus_tag="KPE55_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0493079.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14632..15822)
                     /locus_tag="KPE55_002015"
     CDS             complement(14632..15822)
                     /locus_tag="KPE55_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493080.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(16061..17149)
                     /locus_tag="KPE55_002016"
     CDS             complement(16061..17149)
                     /locus_tag="KPE55_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493081.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         5500 a         2983 c         2075 g         6707 t
ORIGIN      
        1 tagtcaagaa tattttattc tctaaatcta tgttttatta attctttaaa aaactattat
       61 ataaaataat catgataaaa ttagaaattg gaatttttaa tatgcactgt acataaatat
      121 attttatata aaagtttata ttttcaaaca agtaattttt cgaaaattgt ctaaattcag
      181 taaaaatatg gtaatatata cataagcaat ataaatctat aaaaaggagt gttacatatg
      241 atatctaata aaatgcaaac tttagttgca aatagttctg ttataagagc tatgtttgaa
      301 gaaggaaaga aactttctga tatttatggt gaggaaaatg tttttgattt tagtatagga
      361 aatccaagtg ttgaacctcc agagactatt aaagcagtta taaatgatat tttaaatgaa
      421 gaatctccaa atttagttca tggatatatg aacaactcag gatatgagga tgtaagagat
      481 gctattgctg aacacataaa taaaaaagat ggattaaatc ttacaagaga aaatttaatt
      541 atgacttgtg gtgctgctgg tggtctaaat attattttaa aaactctttt aaatcctggt
      601 gatgaagtta ttgcttttgc accgtatttt ggtgagtata aaaattatac agaaaactat
      661 gatggaaaat taatagaagt tcctacaaat atagagactt ttgagccaga tttagatgct
      721 ctaaaaaatg ctattactcc aaaaacaaga gctttaataa taaacactcc aaataatcct
      781 actggtgtca tatattctga agaattatta aaaaatcttg gagaattatt ggattcaaaa
      841 caaaaagaat ttaatacaag catataccta atttctgatg agccatatag agaaataatt
      901 tatgatggtg ctaaagttcc ttgtgtacta aaatactata gaaattcatt tataggatac
      961 tcttatagta aatcattatc tctacctggt gaacgtatag gatatattgt tgctaatggg
     1021 caaatggatg attttgatga tgttatgtct tcattaaatg ttgcaaatag aatacttgga
     1081 tttgtaaatg ctccttctct ttttcaaagg gttattgcaa gaagtttaga tgcagaagtt
     1141 gatgtaaata tttataaaaa gaattttgac cttctatata atagtttaat cgatatggga
     1201 tattcttgtg ttaaacctaa tggtacattc tatttgttcc caaaagctcc aattgaagat
     1261 gataaaaaat tctgcaatga tgcaaaacaa tttaatttat tattagtacc aggttcttct
     1321 tttggttgtc caggtcattt tagagtatca tattgtgtat cttatgataa agttaaaagt
     1381 tctttaccag catttgaaaa attagccaaa ttatataatt taaaataact aaatcaaagt
     1441 aattcaaata attaactacc ttagtataga atacttacat tctatattaa ggtagtttat
     1501 aattttatgt actatatttt atacatacca tattattcca atatgcttga atattcttat
     1561 ctatagtact aatataaatt acaaggaggt gtttgacttg aaaaaaggtt atgtttatat
     1621 aatactgact accttatttt ttagttcaat ggaaattagt ctaaaaactg tcactaatga
     1681 ctttaatccc ttacaaataa ctttatcaag attttttgtt ggcggtttag tcttactacc
     1741 ttttgctgta aaaagattaa gagctcttag tctttctatt acaaaatcag acttaaaata
     1801 ctttgcattt ttaggtttta tgtgtgtagt agtaagtatg tcattgtatc aacttgctgt
     1861 tctaaacaca aaggcatctg tagttgctgt tttgttcagt tgtaatccag tctttgtaat
     1921 gcttttagct tacttaattt tgaaggaaaa aatatatagg cacaatgtaa tttcacttat
     1981 attagaagtt cttggaatta tagttataat aaatccattg catacaaaac taactcttag
     2041 tggaatattc ttaacattat cttctgctat tattttttca atgtatactg tatttggtaa
     2101 aagaaaaacc ttaaaatttg gtggaatagt tgtaacctgc tttagcttta tatttggtag
     2161 tttggaaatg ttgatattgg ttttactcac taaaattaat tttatcgcag atatattaaa
     2221 taaaaataag cttcatgtat ttgcaaatat ccctattttt agtggatata ccttacacaa
     2281 tatgcccatt atgatttatg tattcgtatt tgttacaggt gttggttatg cgctttactt
     2341 tatggcaatg gaagctacat ctactagtct tacatcttta gtattcttct ttaaaccagt
     2401 aatatctcca atactagcat tattaatact taaagaaatt atccctataa acatggttat
     2461 aggaatttta ttgatagtaa ttggttcaat aatttctatt atacctacga taattactca
     2521 aatgcataat aaacacgttg aagccttgta taaggagtag taattgataa tctatcatag
     2581 ttatacataa gacaaaattt attagattta aacgcaatat aaaagagatg ctattataat
     2641 agcatctctt tatttatatt tattttaaat ttattaaaac tctatttagc taaattttgc
     2701 aaaaagctat aagtttgtgg acaagctgat tttaacttac tatttgtatc ttgatttaaa
     2761 tagtaatatg caaatgattc tgcaaaaaat tcttctggat atacacctaa gtagtttaca
     2821 ttacctaaag aacgaccttc tttagcaaat atttgtttga attgtgctga tttagatata
     2881 tcatttaata ctatatggtc tattgcatgt gctgtttcat gtaattctaa atttattgaa
     2941 tcatgccctt tacctttgtt actaaatcct attctaagag ctactgtact tccacctaaa
     3001 cctggaacat catcccaagt ttttccagta ccttcccatc ccttaggaac tacacctttt
     3061 aaataagcat attctttttc atctgttaac tttccactta ataattttat ttttaagtta
     3121 ttttgtttta atgcatttaa atactttcca tctatattag ctaatctatt taccatagca
     3181 ttagcttcgt ttttatcgta atttccagtt ggaaaaacta ctatttgact aagtgtgtct
     3241 ttattttgtt gtatagtagt actatctgca tgtgcactta ctggaactga acttattaag
     3301 aatattgata ttatagctat taataatttt ttacttggtc tcatattatt ccctcctttg
     3361 agttaggccg ggagtaataa atttcaaaat aaaaaagata cccaagagta tctttgtata
     3421 tacaaaaaga aatttttact cttttcggca actggctggc atggtttact aaactttagc
     3481 ccctatagct ttgcgtgact aggtttccct agttttgcct catttaattt tatatttata
     3541 ttatagctta tatcttttta attgtctata tccctttgga acttttttct aaaaagttat
     3601 tattttttta cataaatctt caaaaaaaaa tatcttctaa caatattttc attattaaat
     3661 attgttttag aagatatttt ttaatataac ttatattttt ataatgttag ggtctaattt
     3721 gtatttttat ttcttcttaa tacgataagt cctacaatag ctcctataac caaaagtatt
     3781 tctccaataa ttgtagtact atcccctgtt tttggtggta ctggtggatt taccaaagta
     3841 tcatctttaa ctttattatt tttttcttct gacactgatg gctttactac ttctggtatt
     3901 ggaataatct gtttaacagg agtttctatc atctcttctg atggtggtac tggcggattc
     3961 aatacctctg gtattggcgg atttattata tcttctgatg gtggtactgg tggatttata
     4021 actatagtat tctctgtatc agatgaactt gatggtttcc ttggtttatc tgtgcttggt
     4081 ggtactggtg gatttactat tggttcatct gtatctggag gtattggtgg atttactggt
     4141 ggattttctt gttttagatt ttctatattc ccaatatcta ctatcttacc aatatcttca
     4201 ttaccaattt caaccttaac tttatccttc agtttttcat atccacgtgg agccattatc
     4261 tccactaaat aatatgttcc tgggtttttt acactagatt gaattatacc atcttcatca
     4321 ctaactaaat ccttaactac tatatttcca aatgcatctt ctaaattgaa cttagcacct
     4381 gaaagtacat ttttatctaa agcatccact tttttagctt caaaagcata accttgctta
     4441 gtagtctcta ctcctggagg acttgaacca ttaatctgag ccacatttcg tatagtctcc
     4501 ccaactttta catttgtgaa gtttgcatca aatgtaaccc aactttgcac aggttcccct
     4561 tcttttatct gtttaaattc gtatttattt actaatctta ttgtattttt tccatctcca
     4621 gcttcttttt taagttcaag attatcagaa tattttatat tagatatttc tagaactgag
     4681 tttacttcat ctaaaagatt tacttgtcct gcatcataaa ctccataagt tgacatattg
     4741 attttgtatt taacaatctg gttatcacta ctgtttaaaa cttttttatc aacttctttt
     4801 tttgcgctta ttaatccaga atttttctta aagtttatct catcattttt atcaatttca
     4861 ctattatttg catcaagaac agctctattt atatagctat cttcaatact aattaccttt
     4921 aatgtataag ctataaaaaa ctttgattcc acttcttctt tactgagatt aattgtaaat
     4981 ccagttataa ctttatctac ttgcccactt tcatttcttt tttcttctac tttatatgaa
     5041 atattattgg caattttttc tttactccaa ggagtccagc tcccattcat tggaatttct
     5101 acaactctaa aatcatgccc agcttctcct gaagtagtta atctcatgcc ttctggtata
     5161 ttatctgtta taacaacatt acttattggt gatttatcat catttattga aatctgatag
     5221 tttattactt gaccaacttg agtaatattt gttgctcctg aatgtccttt agtaagaaga
     5281 taatcatctc ctctatcaaa tatacttaca gtagcatcat cttcttgtat cttcttacca
     5341 tcagattcaa tcctagctac attcttatat ttattaccat gttttgcatt tttgtcactg
     5401 attctagcct tatacttaac agtatacgat ttatctatat ttccaaaatt aactgaaata
     5461 gaatttttac tctgttcaat attaaaatca ctaacttctt ttccatcact tgttactaca
     5521 cttacagaac catttatcaa tgtcaatcct tcaggaagct catcatacaa aataatgtct
     5581 tttaactctt cctgcttttc atttacttgt atactgtaat ttatatcttt atttttaact
     5641 ggcataaata cagtagttga tttttttcct tcaacaagct ttttaagtgt tcctttagca
     5701 tctggaacat ccgttttatc tccaggtttt atttctacat ctatatctcc acttccactt
     5761 tctatttttc cagtagtttc aatatgtatt tttgcattag tatcttcatc aaccttttta
     5821 acatatgctt ccaaacctat tttaccttgc attacacttt ggccattagg taattctcta
     5881 aatgtaagtg tatactctcc attactttct gttttatcaa agcattcact tgtataatct
     5941 aaattaactt tattgaatac atctggcacc ttaaaagtta ttgaatctcc tgcttgtatt
     6001 ccttttgatg ttggagtata agttaaatta aatgatattt ttttcattga ctccgtttct
     6061 attgtattgt tattaatttt tacaacaaat ccatagtctt cattttcttc attaagttct
     6121 tcttctaaaa ctttcaataa attttcatct tctaatagat tatcttcctc tggagttttt
     6181 tctgtatttt tattgtctag tggtttttca tcttctaaag cttgttctga ctttttatta
     6241 tctgttgact tatcttcttc ttgtatctgc tctggctttt tattatctat tggcttttct
     6301 ccttctggag tttgctctgg ctttttatta tctgttgact tatcttcttc tggggtctgt
     6361 tctggttttt tattatctgt tggtttttct tcctctgggg tctgttctgg ttttttatta
     6421 tctgttgcct tatctttctc tggagtctgt tctggctttt tattgtcgct ttccttctcc
     6481 tctccagttg atgaattagt tgaaccactt tgaatctgac tattctctcc taattctgaa
     6541 gcataagata cagttggata tattgtagac accaccatac ttaaaatcat aattaaacta
     6601 attaataatg cctttctatt tcctttcttc atgtaaatct caccccttta ggttacctat
     6661 aatttataat ctaatcttcc attaatattg tatataaagt aatttttact aaaaaattat
     6721 acaaaaaaag ctatgaaaat aaataaattt atattcatag ccggttgcac cactgactca
     6781 gtatatccat aaggtgccca ataatagaat atacttcaac atctctataa tatttcataa
     6841 aaacaattaa aattgcttta tttatattaa aaatacaatt atatacttct attatattat
     6901 gcctctatac tattgtctat cctttttaca ttttttacct tatgatgaga ttaattaata
     6961 attaaataaa atccttatct acaacaatta cacaattata ttcaggattt atctccttaa
     7021 ctaattttgt tatatcttct tttaagtcat cttctgacat gtttttggaa aattcggaag
     7081 gattaactac tatatcaaat attaaattct tcttgcttcc ttttccaact attctaaaat
     7141 catgcataga ttttacaaga ctatttttct taagtatttc ttcaagttct tttttggctg
     7201 acataacttc tttatcttca agacatattg ggtccatgtg aattactaga tatatatcta
     7261 atttttcaga tatttctctc tctgcagtat ctataatttc atgtatttcc attatatcta
     7321 tgttggatgg aatttctgca tgtatagatg caatacatct accaactcca tagttatgaa
     7381 taattaaatc atgtgtacct attatatatt tataagataa caatagctct ataatagaat
     7441 ttaccatttc ttcatccggt gcttccccta aaagaggatt tatagtatct tttacaagag
     7501 agaaaccagc ataaagtatc actaatgata ctacgatacc aacatatcca tctatgggaa
     7561 aattagtaaa tctagctaca ataaatgata taactacaca acttgaagta aatacatctc
     7621 ctaaagcatc aagtgaaact gcttttaagg ctgaagaatc aattttattt cccataaatt
     7681 tattaaaacg actcaaccag attttaatca ttatagatgc aattaaaagt ataagtggta
     7741 ttacttcaaa ttttacagga attggattta ctatcctttc tatagatgac tttacaaatt
     7801 gaagtccaac caacatcacc ataaatgcta ctattaatgc tgataaatac tctattcttc
     7861 catgaccaaa agggtgttct ttatcagctg gcttacttgc taatttaaaa cctatcatag
     7921 ttattgctga agatgccata tcagaaagat tattaaatgc atcagccatt atagctatac
     7981 tagatgtgag cattcctatg aataccttta tcacaaataa aagtaaattg gataatatac
     8041 ctactacacc agctacataa ccatacttat ttctaacatc tgtattttga acgttttcgc
     8101 tatctctgat aaatgttttg actaagattt tggaaaacat gaaacttacc tccctgccct
     8161 ttattttatt aacaaatttt ataatctata tctttattta tgataacata atttatatat
     8221 aaaattctta aaaatcactc tacttcagta aaatattatt ctatatatct atttagatac
     8281 gaattttata tttagtaaag tttttatggc ttagtgtctg tttaatagat tctatataag
     8341 tttttacaaa gattatttat tatgataata tcatttttcc caagtattac ttagaatatg
     8401 atttttttgt tttgtctgta catattttaa atttatacta tatttatcta tattttataa
     8461 ccttatgata cactataact ataagttttt aaatggaaag gaatgaaata aatcatgttt
     8521 tttatcagtt atatttacac aaaatcacct attgaatttg atactccttt gcaaaaagag
     8581 gcatacaaaa tattacaaaa attagatatt gactttgaat gtgttgacac agatgaagca
     8641 attacaatgg aagattgtgt ttagattaat aaaaaattaa atatgaaaat ggtaatataa
     8701 aacaacatta aacaaacaaa aaccgcaaag ttacaatata tagtactttg cggtttcata
     8761 ggaattaatt tttactatta aagttcatag ttctactatt tttcattgtt ctaagaatta
     8821 gctttacaaa ttcatttgac aataggatag tggatgcaaa tagtattatc tttatccaca
     8881 tatcaaagtc caaagaaaca gaattaaaga aactctggaa cacttgtgta aatattattt
     8941 gaattatccc tgtaactact attatttgca atgccagttt atttttaaag aaatttggta
     9001 ttgtactatt taaaccaaac tctctacaat ttaatgcatt aaaaagggca ctgaatgcaa
     9061 ataaagaaaa tataacagta ccttgttctt ctgatgtagc tcccaatata ttaaaagctg
     9121 attgagtaaa tacaattgta attataagaa tagcatttat aataatattt acaaacatag
     9181 acctagctat tattccagaa tgtctattta ttggttttct tttcaataca taatctctaa
     9241 caggttctaa tcctaatgct agggctggag gaccatccat tattatattt acccataata
     9301 attgtattgt agtaaatggc atatccttac cagtaagttg agatattata gcaattataa
     9361 atgctactat atttacagta agctgaaact gtataaatct ttgaaagttc tcatatatac
     9421 ctcttcccca ttttattccc tctactatag tactaaagct atcatcagtc aatattatat
     9481 ctgctgcatt tttagatact tctgttcctg aaatacccat tgcaattcca acatctgctt
     9541 gactaagtgc tggggcatca tttataccat cacctgttac agctacaact tcgccacttt
     9601 tttgaagcgc tgaaactatt ctcatttttg tatctggttt acttcttgca acaatagata
     9661 ttcctttgat ttcttctctc aattctttat ctgtcaatac atctatatat gttgcttcaa
     9721 ctgccttttt cccatcattt aataatccta attcatttcc aatagctgtt gctgtattta
     9781 tattatctcc tgtaagcatt tttacatcta cgcctgcatt aaatgcttta tcaatggaat
     9841 ctttgacccc ttctctcaat gggtccacta ttcctacaaa tccactaaaa actaaattat
     9901 cttccttcat ataacttctt gaatttccta ttaaattcaa ttcaccttct gaagtcactg
     9961 caacttctgt tttagaatta ctcatttttt tataagcaaa tcctaatgtt ctcatagatt
    10021 taatttgtaa cttcttaatc tcatctaaaa tacttttttc tacttttgga gttattggaa
    10081 caatattttt tccttgttgt acataagaac atttttttaa taatacttct ggagcacctt
    10141 ttgaaagtaa tatatctgaa tcctcttgac gaattaatgt tgacatcttc ttttcttctg
    10201 aactaaatgg tatttgtgat atcaaatacg tttgttttct catttcatta tagttcttat
    10261 cattatgata taaaagtaat gcacactctg ttgcgcttcc tatatattta aatgaattat
    10321 cttctttttc tatatctgca gttgaattta ctatacagtt ttgttcaaaa tagctattac
    10381 tttgatactc tcctccactt atatatttac catcaacata tgctacctct accatcattt
    10441 tgttttgtgt taaagtccct gttttgtcgg aacatattac agaaacagaa cctatagttt
    10501 cacaggcttc tttctttgtt actaatgcat ttatctttgc catcttttgc attgttatag
    10561 ctaaggtaat atttatcata gttggcaaac cttctggaac agttgcaact attaatgcaa
    10621 tacaaaccat atatgcattt ttagcaggtt ctaatgattg taagaaagat aatacactgc
    10681 ttgtatctaa aattaacata ccttttctct gcatctgtat aatcataaac atacataata
    10741 aagtagctat tacgcctgat attcctgcta ttttagcacc caacttccct aatttaattt
    10801 gaagtggagt ttgctggtct tcatttgata aattctgtgc tatcttaccc atctctgtct
    10861 tatcaccagt atgagtgaca accattatgc ccctaccata tgctacaaga gtccctccaa
    10921 ataccatatt gacttgcttt gcaggtattg catcctgttc gattatttct gttttagaat
    10981 aaactacttc catatctaat acagcatcag cattcttagc tacatcatca gattctcctg
    11041 ttagcatatc ttctctaagt ttcaaattta tagactgtat aagtcttcca tctgctggta
    11101 tcatatcccc agtttctata tatacaatat ctcctggtac taaatcattt tttgatattt
    11161 taattatctt cccatttcta agcaccttta cttctatatt ttctgttaat tttgacaggg
    11221 catgagctgc ttttttagat tttccttcag ttattatgcc tatagaaatt ccaataaaaa
    11281 tagcccctaa aatacctaat gcgtcatgta attctcccac aaaagaactt attactgctg
    11341 ctcctatgag aattaatatc ataggttctg tcaaactttc tcccaactcg tcccaaaatg
    11401 ttctaccttc ttttatagta aattcattta atccatatct aagcttcctt tcttctacct
    11461 cattatcatc aagtccaatt tctggatttg tttttaagta ttttaatact tctttggtag
    11521 gtttattata atacctcata actttatccc ccttgttaat tatctataag gaattatatg
    11581 cttatgatag cttgtatatt acttttcaaa aaaaataagg tgttgattaa tcaacacctt
    11641 atagtggtta tatattatta aattttatgt tacaggcttt ataaattgtg agctatacaa
    11701 atcaaaatat ttaatcttca aaataccctt ctggatataa tggaaactta gatataagtt
    11761 cttgtgcctc tgctttacat tcatctaata cttctttatt atcaatattt tctgctacct
    11821 tattcattat ccctgctatc tgtataactt ctttttcttt cagacctctt tgagatattg
    11881 atgtaagacc tatacgaaca ccactagtta caaatggtga ttgtgggtcg tcaggtataa
    11941 catttttatt aactgttatt cctacatatt caagagcttt ttcaaattgt tttcctgtaa
    12001 gattcttact tcttaagtca accattacta tatgattatc agtacctcca cttacaatac
    12061 taaaaccatg tgaaatcagt tcagaagcta gagtctgagc atttactaca acttgctcca
    12121 ttatcttttt aaatttatct gtacttaaat ataatagtga aaaggctttt gctgcaactg
    12181 tattaagatg tatagaccct aatgttcctg gaaaaacacc tttatcgagt ttctttgcat
    12241 gttctgcttt acaaagtaca ataccacttc ttggtccaca gaaagtcttt gtcgtagatg
    12301 aagaaacaaa atctgcataa ggtacaggac taggtattac tttcgctgcg actaatcctg
    12361 caacatgtgc catatctacc ataaagtaag ctcctacttc ttttgcaacc tttgatattc
    12421 tttcataatc tattaatctt ggatatgaac ttgcccctgc aattataagt tttggtctac
    12481 attcttttgc ctttgcttca agagcatcat aatctatacg tccagtattt gggtctaccc
    12541 cataaaattc atatttataa atgtcatgta aaaaattaac tgcgctacca tgtgtcaagt
    12601 gtccaccttg gtcaagacgc atacttaaaa cagtgtcatt tggttttaat attgatgaat
    12661 atacacaata gttagctgta gaacctgaat acggttgtac gtttacatgt tctgctccaa
    12721 atacttcttt tgcacgtttt attgctaggg tttcaagttt atctgcttct tcagaacctg
    12781 cttgaaaacg agcccctgga tagccttctt ctgttttatt tgtaaataca catccactta
    12841 attcaagtac ttctgttggt gctgtacttt cagaagctat catctcaata ttgtgttctt
    12901 gtctaaccaa ctcatcagct acaatcttgt ataactctgg gtctgaaatt tttgttgttt
    12961 ttaacataat aatcctcctc ttaattaaag aatatatttt attagtttaa tcttcattga
    13021 taaatttatt ataatggtct ataaaacgta agatattcct ccaccaggtc ctaatggtat
    13081 gccaataccc atgaatagta acaatattat tatttctatt aatgtaaata ccactgagta
    13141 tggaatcata ttcgatatga cagtacctat acctatatct ttatcatatc ttctggcaaa
    13201 ggccaatata actggaaaat acggaaaaag tggacttatt ggatttgttg aagcatctcc
    13261 aatacgataa gctatttgag ttaatgctgg attaaagtct agtagcataa acatgggaac
    13321 aaatataggt gctaaaatcg cccattttgc agaagcactt cctacaaata aattgataaa
    13381 tccagatagc aatataaatc ctattatcaa tccatagcta ggtattcccg actctgctag
    13441 taattttgct cctgttattg ataaaattgt tcctaagtta ctaagattaa ataaattaat
    13501 aaactgggat gctataaatg ctaggactat atatccaccc atatcactca tactactacc
    13561 catcatagat actaaatctt tgtcactttt tatcttctta cttacttttc catacactaa
    13621 tcctggagtt aaaaatataa tagtgattat aggaactata cctttcatta aaggtgcatt
    13681 actagatagt atagaaccag tctctggatc tttcataaat gcattgtcac ctatacataa
    13741 ggcaactatc actactacta caactaacaa tgataataaa gcgtatttta tacccttatt
    13801 ttcaagttca ctcaattcct gtatatcctt atcatttgtt gaatcattat attttgtaaa
    13861 acgaggagct ataaattttt cagttacaaa tgctcctgca aaagttaaaa ctatcgctga
    13921 tataaaagca aaataaagat tcattgctgg attgccttgg taagatgggt ccatcatttg
    13981 agctgctggt attgtaaatc cagctaacaa tacatctgta gtactaagca taacatttgc
    14041 tgcaaatccc cctgcaacac ctgcaaaagc tgcaaacata cctacaagag gatgtcttcc
    14101 aattcctaaa aacacaagtg ctgcaagtgg tggtaatatt ataaatccta catctgctgc
    14161 tgtatgtgat aacatacctg caaatacgat tgctacagtt acaagttttt gtggaacttt
    14221 acttataccc tttttcatta acacttccat aaatccagat ttctcagcaa cacctgctcc
    14281 aagcattgta accagtacta atcctaaagg agcaaatgac tggaagtttg taactatgct
    14341 acctaaatat gattgaatct gctctttctc tagtagattt actaccttta caacttcttt
    14401 tgtagaagga tgtatttctt ctactcctaa attagatata actgctgaca atattacaac
    14461 aattatacat agtaataaaa atatggttac tgggtctgga agtttatttc ctgctctctc
    14521 tacaacattt agtgccctca tcaataatcc actatctttg tttttttcct gtgaattaat
    14581 tttcctcaaa ctaatacccc ctcataaact ttatctccag aatgctttat cttaaatttt
    14641 attttttaat ttatccatat aatttataac agactgacat agtacatttg tacctaccaa
    14701 tatagaattt ttatcagctt taaatttagg attatgccac tgatatatac aatcttgttc
    14761 tttgttgcct acccctaacc agtaaaaaaa tcctggggct ttttccatga atatagaaaa
    14821 atcttctcct cctgtagaag gtattggatc aactatagca tcttctgaat acaaatcaca
    14881 cacagactta caagctattg cgtacatctc tttactattt ataacagctg gtaaatcaaa
    14941 taaataatct agctttgcag ttgcttgata tccacgagct atctcacaaa ctatattttc
    15001 taaattttct gatattttct ttcgtacttc attactaaag cttctacatg tgcctgtcat
    15061 atctaccttt tctgaaatta cattatttgc agtccctcca tttatagtac ctattgatat
    15121 aacagctgat tctaatggac ttatgtttct gcttactatg gtttgaatac ccattataat
    15181 tgcacttgat gctactatag ggtctattgt tctgtttggt atacctccgt gacctccaaa
    15241 tccatttact tctatcttaa tagtgtctac tgctgccatt aacccaccta acttaacacc
    15301 tatttttcca caaggtatat ctggatgatt atgaagtcca aatatagcat caacttttgg
    15361 attttccatt actcctcgtt ccactagcat cttcgcacct ttattgactt cttcggctgg
    15421 ttgaaatata aactttacat ttccatgaag actatctcta atatgagaca atacatatgc
    15481 acatcctaca aggcaagacg tgtgtatatc atgcccacat gcatgcatta taccatcata
    15541 tctggattta tacttaatat caacctcttc atatattggc aatgcatcaa tatctcctct
    15601 tagtgctact gtgggaccat catacttccc ttttaataat ccaacaactc ctgtttccat
    15661 tcctaaatct aatatttcta tgtctaaact tactaataaa tcttttatag cttttgtagt
    15721 cctaaattct ttaaaactta attcggggtg catatgaata tctgtaaaaa aatctaaaat
    15781 atcttcttta tacttttctg agattaaaat aagttcatgc atactaacac ctctccaaaa
    15841 ttttattttt ttatttttta taatttaact acaaattata taagttaata atctttataa
    15901 agatattttt tataaattta attttagttt atatttacat tttactatat tattttaatt
    15961 aaatatatta tcatgttttg caatttattt aactattttg actttttaaa ttcggattat
    16021 ataaaaaatt tctttaaata aaaactatta ataagtggct ctattcctga ctttgtgttg
    16081 atttttttct acatgtaatt gctttataca ccatacagac taaaattaaa agccccatat
    16141 aacctgtata tggatataaa atgtttacta atttaccaaa cggtaaaaaa cctcctatac
    16201 aagccaaaat agctattact gttgttacca atttaaattt aggatgttca tcttcaacaa
    16261 ttctattggt tacagaccat aaaagtggaa cagctgttgt atagatacct aatattaatg
    16321 caactgaaaa acatacacct acaatcggag atatattatc tgctaaataa agtgatggta
    16381 tttctttcat atacaaattt cctatatctg aaatcatagc caaatacata actataccag
    16441 cagtcataaa cactattcca cctaataaac ctccccaaaa agcatccttt ttactttcaa
    16501 tagaagctcc cattcctgtt aaaaaaacaa tcattacaat aatatttaat gtattatata
    16561 ttacaccaga aacgattgga ctttctgatg cttttgtaac ttcaatattt ttcatagctt
    16621 ctccaaccat agataaaccg tcaaaatgct taaacaagct tattaatcca acagcaattg
    16681 taaatactgc tatcactggt ccaatacgtc caagtatgct tactaatttt tctaaaccca
    16741 tagacactgt aattaaagta gcaacagcca tcaatactct tcctaagtaa ggatttatac
    16801 catagtattc agttaaagtt gagcctgcac ctgaaatcat tactataaga atcccaaata
    16861 aaaatattgg actaaaccat tcgtaaaatt ttcctaaaat cttcccacaa taaaaagtgt
    16921 aaattcttgt tggttcttta agttttaatt ctctacctct agttataact tcagcaccaa
    16981 gaaatgaaaa caataccatt gatattaatg ctccaattaa acccatatac ccttgtgagg
    17041 caaaaaattg tagaatttct tgaccagtag caaacccaga accaattgct gttgctagat
    17101 aagcgcctgc aatacagata atatttttca tattcacttt gtctgacata ttaaattacc
    17161 tccattataa agttaccttc ctttaagaaa tataaactct cagtgaatta aatgaaaatt
    17221 ctgtattttt aaaacagtat ttaattcact ttttttattt tgtat
//
LOCUS       DAELNY010000072        17115 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.072, whole genome shotgun sequence.
ACCESSION   DAELNY010000072 DAELNY010000000
VERSION     DAELNY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.072"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..205
                     /locus_tag="KPE55_002017"
     CDS             <1..205
                     /locus_tag="KPE55_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493082.1"
                     /translation="KYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEV
                     IQMLKYGDTKVGRNFIRYSLRSI"
     gene            316..1458
                     /locus_tag="KPE55_002018"
     CDS             316..1458
                     /locus_tag="KPE55_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0493083.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            1912..3303
                     /locus_tag="KPE55_002019"
     CDS             1912..3303
                     /locus_tag="KPE55_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0493084.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            3556..5349
                     /locus_tag="KPE55_002020"
     CDS             3556..5349
                     /locus_tag="KPE55_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0493085.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(5430..6812)
                     /locus_tag="KPE55_002021"
     CDS             complement(5430..6812)
                     /locus_tag="KPE55_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0493086.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            7070..7417
                     /locus_tag="KPE55_002022"
     CDS             7070..7417
                     /locus_tag="KPE55_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0493087.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            7435..8469
                     /locus_tag="KPE55_002023"
     CDS             7435..8469
                     /locus_tag="KPE55_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493088.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(8518..9567)
                     /locus_tag="KPE55_002024"
     CDS             complement(8518..9567)
                     /locus_tag="KPE55_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0493089.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            9842..11377
                     /locus_tag="KPE55_002025"
     CDS             9842..11377
                     /locus_tag="KPE55_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493090.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(11562..12248)
                     /locus_tag="KPE55_002026"
     CDS             complement(11562..12248)
                     /locus_tag="KPE55_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG0493091.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            12373..13755
                     /locus_tag="KPE55_002027"
     CDS             12373..13755
                     /locus_tag="KPE55_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0493092.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            14157..15425
                     /gene="eam"
                     /locus_tag="KPE55_002028"
     CDS             14157..15425
                     /gene="eam"
                     /locus_tag="KPE55_002028"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG0493093.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(15518..15874)
                     /locus_tag="KPE55_002029"
     CDS             complement(15518..15874)
                     /locus_tag="KPE55_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493094.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(16025..17023)
                     /locus_tag="KPE55_002030"
     CDS             complement(16025..17023)
                     /locus_tag="KPE55_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0493095.1"
                     /translation="MISLLMSLLTPFIIAFVLAYIFNPLVKFLESKLNFKRIYSLLLT
                     YGVLIVILISFILFTVPSIVNSLADLVAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKE
                     YGDKIMSVMPKFSNLLIGSLGGIFSTTFSVGKFIVQFLLGFIICFYILLEKEKFLLFT
                     KKVVYISLGKKYGDFIIELCQSLNLNIGKYFTGKILDSFIVGVLSGIGLYFLKSEYAL
                     LFGTLMGVMNMVPYFGPVIGMAPVVIINLFSNPTIALTSLIYLIIIQQVEVTFIEPKI
                     VGGQLGLSPFFTILAVTVGGGFFGIPGMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         6739 a         2064 c         2776 g         5536 t
ORIGIN      
        1 aaaatataag tctataatca aaagaggtgg aaagaaaaaa gttgaattag taaataaaaa
       61 aggtgacatt ttagaaatat ttaagtcttt agaagaatgt gccatttatt tgcaaaagga
      121 aaagaatgaa gttatacaaa tgttaaaata tggagataca aaagtaggaa gaaactttat
      181 aaggtatagt ttgagaagta tttaatttca ttttaaattc ataagagttt catttctgat
      241 tcacacattt aatattattt taatgtaaaa taataaaggt gaagtattgc acaattaaaa
      301 ttaaggaggg tattaatgtc tgaagaaaaa aatccaaaag aaaatacatt agatgaatct
      361 aaaaattcag ataatgtaaa ttctaaaaaa gatgatgtgt cagatgaaaa aaatactgat
      421 aacaaagtta aaaaaaggga aataaagcta aacaacaaaa cacttaaagc tattgtagca
      481 attgttgcag taggagctat atgtttaggt ggaggctatg tttttggcaa agaagctgga
      541 agaaaattgc ctgctacttc aaaacactat aatagcagtg aggtagtagc tactattggg
      601 gataaaaaaa taacaggtga aatgttaaga aagagaatgg agccattatt ttatttaaat
      661 gcaaaaacta ctatgactga tgacgaaata cgttcatatg aatcaaatat gattgaaact
      721 atgattacat ctgatatatt atcctctgaa gcagcaaaag agaaaataag tataactaat
      781 gaccaactag atgctcagta taataattta atgagtagta tagaatcagc aatgggaatg
      841 actaaagaag agtttttaaa acagtttgat ttgactgaaa aatatataaa agatgatatg
      901 aaaaaagagc tagtagcatc aaaatattta aatgaaaaat caaaagttac tgataaagaa
      961 gcagagaact attacaataa aaacaagagc aattatttac aagttagagc ttcacatatt
     1021 ttaataaaaa ctgttgatga taaaggtaag caagttagta gtagcaaaaa agctgaactt
     1081 aagaaagaag ctgaagaaat acttaaaaaa gcccaagctg gggaagattt tgctactcta
     1141 gctaaaaaat actctgaaga tagtagtgca gaatcaggtg gagatttagg tttctttggt
     1201 aaaggtcaga tggtggaatc ttttgaaaaa gcagcctttg cattgaagaa gggagaggta
     1261 tctaacaaac ttgtagaaag tgattatgga tatcatataa ttaagaaaac tgatgaaaaa
     1321 tatcagccat ttgaagaggt aaaatcagat ttagtaagca gtttaacatc agaaaaacaa
     1381 aacctactta tacaaaatct aaaagaaaaa tataatgtta aaattacaga taaatatcaa
     1441 atgaaagcta aagaatagat aaagtcaatt tattaaaaaa aagaaaaatt aactgtatgg
     1501 ataaaagcta tttttactct caatatagag agtataaaat agcttttatt attttgtaat
     1561 aaaatctatt tatatatatg aagtgataaa gaaataacat gcaaaaaggt attaaaaaga
     1621 aatattttta atttgaattt tttcaataaa aatttacaaa aatgaaaaaa taaacaaaaa
     1681 agaattgaag atttaagttg ttatttaaat atatatatat taagattttc tgctgaagat
     1741 actaaaaaaa taatattttt cataatttta aaaataataa taaaaaaatg aaaatacagg
     1801 ctataatcta ttgagtaaac atagaaaaga tgatattata agtatgtgct aaaatggtta
     1861 aagcaatatt aatattaaat ttaactataa aacaaaaggg ggcgtttttt tatgtcaagt
     1921 acagaaagcg tatctaaaaa aagtggtcat ccgaagagtt tttggcttat ttgcatgaca
     1981 attttgtggg aaagatttag ttattatggt ttaacagctt tattggtatt attttttaca
     2041 gcaagtgtag ctcaaggtgg tttaggtctt tctactcaag aagcaacatc tatgtttggc
     2101 ttatttacag gtcttatata tcttacacca ttagtcggtg gctggttagc agataaagtt
     2161 ttaggtcaac aaaaatgtat ttctataggt gcacttttaa tagcaatagg agatttcgta
     2221 ctatttgctt catctacatc tattgcaatg gtttgggtag gtctatcaat aattatttta
     2281 ggaaatggtt tttttaaatc aagttgctca aatatagttg gggatatata tcctaaaact
     2341 gatgtgtcaa gaaaagatgc agcatacagt ttattctata tggcagtaaa tgtaggagca
     2401 tttatagctc caataatatg tggattgctt tctgataatg tatttgcagt aagaggagca
     2461 agtggagata tagtatctta tggatataag atggcatttt taatttgtgg tataggcatg
     2521 atgttaggta tgattatatt tacacttctt gcaccaaaat tattaaatga agtcggaaaa
     2581 tatcctgtga ctaaaggtaa taaaggtgaa aaaattgagt atggaccact tactaagttg
     2641 gagaaaaata ggataatagc aatggttatt ttatttttat ttgtggtctt attctggaca
     2701 gcatactatc aaacacaaag ttcttttatg atttatgtaa gggataatgt aaatagaact
     2761 ctatttggat ttgaaatgcc agtagcatgg cttacatcac taaatgctat attatgtgtg
     2821 gctttatgcc cagtattagc taccctatgg gttaaattat ctcataccaa aagaggtgat
     2881 ttctctattc cagttaaaat gggattagga atgataataa tgggtgttgg gtttatagtt
     2941 atggtatttg ctacattagc taatggtcca gatggaatgg taaaggctag tatattgttt
     3001 atagtattga cttatatatt tacaacagta ggtgaattat tcttatctcc tataggactt
     3061 gcaatgttca ataaattagc tccagctaag tatgctacgt tgtctatggg agcctggtat
     3121 ctaacaagtt tttttgcaag tttattatca ggaaagattg ctggttttac agccacactt
     3181 ggttttttac agatatttgg aggaatagca acagttttaa taatatttgg tttaatatta
     3241 ttgggaataa gaaatgtatt aacaagatta atggctatgg atttactaac aaaagaaaaa
     3301 taattcatga cgaattattg taaataaatg aattacttta aataaaggaa gttacattta
     3361 aatataaaat taaatgtaat ttcctctttg attatgaaaa aaagctttac acaaatagat
     3421 attataatta aaattataat tgtgataatt agagtgtata agatttatat aaattataca
     3481 aaaaatattt atgtaataaa ctttacatac ttatatattt agttattcaa actattattt
     3541 cgaggaggtc ttgttgtgaa tataaaagat agattaagtg gattaagaaa atttatggaa
     3601 gaaaaaaata tagatgccta tatgattcca tcttctgata atcaccaaag tgaatatgta
     3661 ggagattact tcaaatcaag agaatttata tcagggttta atggttcagc agggacggtt
     3721 attgtgacta aagacgaagc tgggttatgg acagatggaa gatattttat acaggcagaa
     3781 tctcaattag aaggaagtac aataaaatta ttcaaaatgg gacaagaagg ttgcccaact
     3841 acagatgaat atttatataa aaatattcct gaaggaggaa ctttaggatt tgatggtaga
     3901 gttatatctg ctagagaagg agctacatta gctgaaaaat tatctaaaaa aggtataaag
     3961 atagaatatc agtatgattt aatagatggt atatggccag atagacctgc tttatcagat
     4021 agtaaagcat ttttattaga tgttaaatat tgtggagaaa gttttagctc aaaattagct
     4081 agattaagag aaaaaatgag tgaaaaaggt acttctactc atgtaataac tactcttgat
     4141 gatatagcat ggttattcaa tataagaggt ggagatgtta aatataatcc agttgtactt
     4201 tcttatgcag taataacttt aaaagaagtt tatctatttg ttgatgaaag taagttaaat
     4261 gaagaaatac taaatgaact agctaaggaa aatgttcaaa taaagccata taatgatgtg
     4321 tatgaatttg taaaaaatat agacaaaact gaaaaagttt tattagatgg aactaagctt
     4381 agttatacaa tttacaataa tataccttgt gaagttgaga aagttgatga gttcaatcca
     4441 gtaatgtttt ttaaagctca aaaaaatgag gtagaattag aaaatataag aaattcacat
     4501 gtaaaagatg gtgtagcttt cactaaattt atgtactggt taaagaaaaa tgtaggtaag
     4561 atggaaatca ctgaaataag tgctactcaa aaattagagg acttaagaag agagcaagaa
     4621 ggtttctttg agccaagttt caatacaatt gctgcatata aagagcatgc tgcaatgatg
     4681 cactatagtg caactccaga gtcaaattat aaattagagg cagaaggatt atttttagta
     4741 gattcaggtg gacaatatta tgatggaact actgatataa ctaggactac agttttagga
     4801 cctataagtg atgaattaaa actacacttt acttctgtag caagaggaat gataaatctt
     4861 tctaaggcta agttcttgca tggttgtaga ggatataact tagacatact atctagaagt
     4921 tgcatgtgga atatgggaat tgattatcaa tgtggaactg gacatggtat aggatttgtg
     4981 ctaaatgttc acgaagctcc aaatggattt agatggagag tagtacctga aagatttgat
     5041 agtgcagttc ttgaagaagg aatggtaact actaatgaac caggtatata tatagaaggt
     5101 tctcatggta taagaacaga gaatgaaatt gtagttagaa aagctgaaaa gaatttctat
     5161 ggtcaattta tggaatttga agttgttacg ttagctccta ttgacttaga tggtatagta
     5221 ccagaattaa tgaataagga tgaaaaagat tatttaaact ggtatcataa gttagtttat
     5281 gataaaatat cacctttctt aactgatgaa gaaagagaat ggttaaaagt ttacactaga
     5341 gctatatagt ttgtactaga tacaataaaa atcgaggtat tataatgcct cgatttttta
     5401 ttgtatattt tattaacttt aaaaaatttt taatttgata attctactaa ttttttattc
     5461 attataagta gtataattcc aaatatcaat aaaaatgatg gtatcactat gaagacttgt
     5521 aacataccaa gttggtctat aatagcttgc gtatagccag ataatttatt tgctgcaaat
     5581 ttgcctaaaa accaaacacc cattaggaat gaaatatact tttcaggcga aagtttattt
     5641 accataccat acccgactgg agaaaagcaa atttctccta tactttggaa tgttaaaaat
     5701 ccaactaccc aaaatacact tgctttgtga attgaattag ggtctgttcc tgtttgtata
     5761 actgctacta tcataaacaa aaatgctatg gctaatagta taaaacctaa gctcattttt
     5821 tttggcacac ttaaatcgcc ttttttagtt tgagataatt ttacccatac taaagcagag
     5881 ataggtccaa gaataacaca aaggagacca ttgtatgaat ctatccatga agtaggaatt
     5941 tgaaaattgc caacagtaag atttatgtgt tcctttgtat agagtgcaat agacatagag
     6001 gcttggttat atgctatcca aaaaaatact gtgaaaaaag ctaacaccaa tattactatt
     6061 attctttttt tctcaatcaa agttaaaggt cttgaagcga gcattttctt atattctagt
     6121 gattttctta tagactctct cccgttaata gggtcattat tcaaaggttt ataaaacttt
     6181 ctgtttccaa atataaagaa gaataaacct attagaaccg taattgatga aaataaaaat
     6241 acatatctat atccatacat acttatagat aatccgcttt tcatagcaaa ccattcatta
     6301 gcaactaaac cgcatattaa agggccaaat aaagtaccta gattagtagc catatacgta
     6361 atagagaaag ctccatcttt tcttgggtca tcattggtat ataagtttcc aaccatagta
     6421 tgtaagtttc ctttataaaa acctattcct aatccagata aaaatatcat gatgtttata
     6481 tccatcttac tatttgctat gtatcctaga aaataaccaa ttgaaaccat tagcattcca
     6541 agtataatac agtttttggg acctaaaaat ctatcactga cccatccacc aataactgga
     6601 gagaaataag tccatgctaa aaaagatgca agcattgatg cggattctgc tggtgttaac
     6661 cctaaaccac ctttggagac ttgttcagct aaaaacaata ataataccaa tttcatacca
     6721 tagtatgcaa gtcctacaaa ggtaaatatt gaacagcaga aatataaatc tctaggttgt
     6781 ttaagttttt cttgcgtttt taaaactgac atatgtcgag aacctccaga taaatagttt
     6841 aacaaaattt taccatacta tatgagtata tgtttttttc atatagacgt caacttgtaa
     6901 gataatttaa ttaagtcgaa atatatgtaa aaatagtatc atttttgtgt attcagtaat
     6961 atttttttat gataatatat gttattatta tattaaaata caaacatatt tatggaaagg
     7021 atatgccaat ttagtagtta gattgattgt ttggcaggtg ggatgagcta tgataagaaa
     7081 gaaatctgaa ttacaagtag aagtaaaaga aaatttaaat ggtggtatag gaaaggttaa
     7141 gcttgaaaac ataatacaaa atgaagagct taaaggaaaa ggaagactat ttaaaagagt
     7201 agctttacca gtaggaagtt caattggtgt gcatgaccat acaacagatt ttgaagtata
     7261 ttatatacta aagggaaaag gtaaagtatt tgataatggg gaatttgtag aagtaaatga
     7321 gggtgatgta gtgtatacag ctgatggcga aaaacattct atagaaaata taggagaaga
     7381 agatcttgag tttgtagcac tagttttata tatgtaaaat atggaggagg agtcatgaca
     7441 ggcaagatag aagaaattaa ggacaagata aatagatttt gcaactccaa tttaaatcaa
     7501 gaatacaaag atataagctt taaaattttg caaaaccttt tagataataa taaagagata
     7561 attcattctt ctagagctga tatatggtct tcagctatac taaatatagt tttagaacaa
     7621 aatcttttat acaataagaa gcatccacta catataacaa aaaaagagtt ttcaaaacaa
     7681 ataggtgttt ctttaaatac tataaataat aaatcgtctt taataaaaga tgtttgtagt
     7741 attgactttt taaatcaaga tactatagct atttctaatg tagaattttg ggtaaaagag
     7801 actaattcca aatcaaagtc tgtagattta gatttagaaa agaagaaaat actatataaa
     7861 agatatatta agctatcaca agatgcgaat agtgatattg aatcaattag atatatggag
     7921 gaagctgtaa atataggtaa aagtatgatt actaaagaag ataaagaact tggattttgg
     7981 aaaggtttat ctaccagagc ctatatgata gcacttgaat cccttgctag aaagttagaa
     8041 agtattaata atctaaagga gtccaaaaaa gtattagaat atttaataga aattaatcct
     8101 agtgatgagc agggaataag atacaaatta ttaaatgtat taattagatt aaatgataga
     8161 acttcaataa ataaattatt tgaactttat aaagaagaaa aatctgctac atggatgtac
     8221 agtaaagctc tttattactt taaaaataaa agtatgtttt tggcaaatga ttctattaag
     8281 gttgctagaa gtaaaaataa gtacgttgga ttatatttgt tagattggcg aaatgctttt
     8341 ggtagagaat ttgttacaga ggaagaaaag gcagaagctg tctattacta tgatgaaaac
     8401 atagtgattt ggaatgaagt taaaggttct atggattggc tattaaaaaa aatgttggaa
     8461 ttttcataaa aaaaactact ccttactttt atataagtaa aaggagtagt ttttaattta
     8521 tacatctagt tttaaatctt caggttttat tagacctttt tttctcataa attctgaaat
     8581 caacaaagtt acaatagcag gaagtataaa attaagtaaa agtataccaa cccacataga
     8641 tatacctcct tttccagcac tttgcatagc agttactgtt ccaaattgtc caaccaatcc
     8701 acaagtaccc ataccagaac caataggaat attctccatt ttgaatattg ttgtagataa
     8761 tggtccaagt aatattgaag ctacagtagg tggtatccaa attttccaat tttttattat
     8821 attaggtact tgcaacatag atgtcccaag accttgtgcc acaaggccac caactccatt
     8881 ttctctaaag ctcataacag caaagcctac catttggcaa caacaaccta cagttgctgc
     8941 accaccagca agaccaccta aacttaacat catacaaata gctgcactac ttataggtaa
     9001 tgtaagagct attccaacaa gtgcagagac taaagctccc attattatag gttgcatagt
     9061 tgtagcttgc attattatat ttccaaatga agtcataaaa gcagaaacag ctggaccaat
     9121 aagttcagct acaagtactc caattagtat tgtcacagca ggtgtaacca atatatctat
     9181 ttttgtttct tttgaaacta attttccaac ctctacagca aaaagtgtag cgacaaatgc
     9241 tccaacaggt cctcctaatt cattaccaca agctccaaca agtgcacaag aaaatagtac
     9301 aagtggaggt gcttgaagtc catacgcaat agccactgct atagctggac cagtaacctg
     9361 tttagcaaga gggcttataa cttcagtaaa aagagaaata ttgaaattat ctcctaatgt
     9421 attgaaaatt gttccaatta aaagggacga aaaaagaccg agtgccatat aacttagtgc
     9481 gtcaataaga tacctttgaa cagaaatttc aatatttttt cttttagcaa atgatttgaa
     9541 atttcttttt ttataattga tttccaattt aaaatcctcc tcatataata taagtataat
     9601 ttctttttaa ttaattatat aacttttcag ctgacaagac aaatgttatt tcactaaaat
     9661 tagactattg taatagtaag aaattaacat taattttagt tttaaatttt aaatttatgg
     9721 tttataatta gtatagggat attctgaaat ataataattt tccagtttat cccaatattt
     9781 aacttaggta gattgcttac aaaacaaatg attatatatt aggctaaaag gaagtggata
     9841 gatgatattt gtaaaaaata agaaatacag aaaaattgcc ataattatat ctattcctat
     9901 attgcttgtt gcgcagtact acctttttaa atttggaata cttagaccaa tggtaaaggg
     9961 agtagaaatt gaaattgtag atggggaata tgttaaagat atagataaat atgtagtaaa
    10021 attaaatgaa acagttactt tgtctacagg tgaatatatt aaggttcctt cgtatgctaa
    10081 aaatccaaat atttggttta atgtattgga taactcaggg actttaaaaa tagatggaaa
    10141 taaaatgaca gcattaaagg ttgggtattc ttctgtgggt atcatgaaaa attcaagagt
    10201 attaaaaaag gctactataa aagtagtaga cccagaggta gaatcactag atatggagat
    10261 taatggggat ttaaaacatg ttggtgatag tgctactata gaaagtacaa tagaagttga
    10321 ttatgataag tttaaaaaat catataagcc agtttataag agtagcaatg aaaatatttt
    10381 aaaaatcaaa ggaaataaag taagtgcagt tggagttggt aaagctacaa tatatgctaa
    10441 gtgtggaaat aaagaaattg agagaacatt taggattcaa gctagagttg ctgaaataaa
    10501 tatggatgat ataaaaatag aagttgacca gaatgaaaaa ttgaaaccag atataataac
    10561 ttctccacct ggtttagaac caccaaaaat atattatgag ttagtagatt caaaattacc
    10621 tgtagaaaga gcaataagtt taagtagtaa tggaaatgtt gtagggctta gagaaggtaa
    10681 agatagggtg agaataactt gtggagataa atctaaagta ataacagtaa cagtagtcaa
    10741 agaaactata acgaatgact atatacaaaa cttggttggt tcgcataaaa tagtaaacaa
    10801 taaagttatt attaaacttg aatgggatta tatgaaagat gtctttgatt atgatatata
    10861 tcttaaagat aagttgtcag gagaatcatt taagaaaatt aaatctataa ctataaatga
    10921 aagtgattta ggtaagtcta acaaggtata tgcgactata gaagtagata tgaagggtaa
    10981 tcaaaatata gattttgata tttatgtcat aggtaaaaag gattcagata caactaagcc
    11041 aagtaatgta ataaatatta ccaatggaaa tggaaattca aataatgata ataatattaa
    11101 agatatgagg gtagaaaata tatcagctaa tgttgataga gataataatg tcattagagt
    11161 taactggagt agtatatctc ataaggactg tacttacagt atatatgtaa aaaataatac
    11221 taatggagat ggagaattta ctctatatca aagtgatata cagggaaatg agcatactat
    11281 aagtattcct gaaggagatt taaatctaga tatttatgtt gttgcaaatt ataatggaaa
    11341 tagctctcaa aatagtgatg ttataaatgt aagatagatt tgtatacaaa ttgaaagtgt
    11401 tgagtttatt taaatattaa attaagaaaa aggatagtat agggtaatta tactatcctt
    11461 ttttgttaca atatataggt ttaaaatctc tagtaatata aattataaaa agtcttattt
    11521 acacaaatct atagatattt tttttattaa tcaatattgt cttaaattga acttagtgca
    11581 gcttctattg catgtattac tgtcgtatca aatagtggaa tagaagtgtc attttgttta
    11641 attaaaagac ctatttctgt acatcctaat ataacagcct ctgcaccttg tttgccaagc
    11701 ttatctataa tagataagaa agcttttttt gaagattcag atataattcc taagcaaagt
    11761 tcattaaata ttgtgtcatt tactattata cggtcttctt catttggaat taagacttca
    11821 attccattat taataattac atttttataa aaatcttgtt ccattgtata tttagtacct
    11881 aataaggcca ctttttttat ttctttttcc cttaataccg tagcagttac atctgctatg
    11941 tgaagtaatg gaatatgtat actttcttgt attttatctg ataccttatg catagtatta
    12001 gtacagataa caataaaatc tgcccctgcc tcttgtaatt taattgcagc atcagctaaa
    12061 attttggcgc ttttttccca atttccacta gattggcatt cttcaatttc ttgaaaatct
    12121 acactgtaaa gtatacattt agaagagtgt agtccaccta atctttcttt tattactgta
    12181 ttaataactt gataatatgt aatggtactt tcccaactca taccaccaat taagcctata
    12241 gtcttcatat tatacctctc ttatttattt attttgaaat agctatttac aaatacaagt
    12301 atagcatcta aaatagaagc atacaataat aaaattgtat ggagtacaat tataattaag
    12361 aggagtaatt atatgccaat aaattctttt gaaaactatc ctatgacatg gaaaccaaag
    12421 ttagataacc gaaaaccacc aatatataaa actttagcaa tgttattaga agaagatatt
    12481 aaaagaggaa atttaaatcc tggagataaa ctgcctccac aacgggaatt agcagatttt
    12541 ctagatttaa acttaagtac aataacacga gcatttaaac tttgcgaaga aaagggacta
    12601 atatgtgcaa aagtgggaaa gggaacattc atttcttctg atgtaaatgt aagtaataca
    12661 ttattatatc aaacggaatc aaaagacata attgagttgg ggacagttca tcctccgtat
    12721 gagcaaaata catatattat tgactttatt aaaaatgtac taaaacaacc agaaatggat
    12781 aggtttttac aatacatgtc tccatctggc acatatatgc aaaaaaagtc aattgcaaaa
    12841 tggatagaaa gaaataatgt atacaccagt gaagaaaata tattattatc tacaggaggg
    12901 caaaatgcta tatgtgcaac tttactagga ttatttaaag ctggtgatag aattgctaca
    12961 gattctttaa gtttttctgg aattaagtca atagctaaaa tgataggtat tcaattagtc
    13021 ccaattcctc aagaaaataa tgaaatatcg attgaatatc ttgaaagtta ttgtaaaaat
    13081 gaaaatataa aaggcatata tcttatacca gattatcata atccaacgac acatactatg
    13141 tctgatttat ctcgtaaaaa gatagccaag atagcaaagc aatacaatat aataattatt
    13201 gaagatgcta taaatagcat ttttagagac ggtatacaaa ctcccatatt ttctttagct
    13261 agtgataata ctatatatat ttttagtaca tctaaatttt tatgtgctgg tcttagagta
    13321 gcatttgtag ttgctccaaa acgatatata gaaaatttag aaaatgcact atataatatg
    13381 aatctaatgg tttctccatt tacagcagaa atagtacacc gtttattata ttctcctatt
    13441 atagataaaa ttatagaaga gaaaagagat gcaattatag aaagaaatga agttgctgat
    13501 aaagttttgt cagattataa tttgattggt gataagaatt gcagttttcg ttggttactt
    13561 cttcctgatg aattggatgg aaaatcattt gaaatttctg caaaaaatct aggagttcaa
    13621 gtatattgtg ctgaacgatt ttcagtagga aactctacag ttccaaaagc agttcgtatt
    13681 tgtgtaacag ctccaaaaga tgtagaagaa ttagaaaaag ggcttaatat aataaagtca
    13741 cttcttaaga gttaaattta ttttactatt atttaattaa ataaataaaa aggcggagaa
    13801 atttattata ataaacctcc gcttttatta tttaatgaaa tactttactt tttattaaaa
    13861 aatttaattt taataatctt gaaaatataa tcgttttatg gtaaaataat tcttaaaaat
    13921 agaaaaaatt aatcatttgt taaaaaaaat gcataattta ttgtaattta ttgacgaagg
    13981 ttgaaaaata ttcataaatg tgcgaaataa cagaaaaaat gcaaaaatta attcaatttc
    14041 atttgttata aaatctatat ggcaaaatta aatctgccta tcagaatgat ataaacacat
    14101 cagaaaggag gacgctatca aataatacaa gtagttaact ttgatagcaa aaaattatga
    14161 atgaacaaac tagaatatcc ttagagagag ctgctgaatt aaaatcaaaa attgatgatt
    14221 atattcaggc tagaaaaacg attaacagag gtcttgaaaa agaagaagag ataaataaac
    14281 gaaaacagaa aatattaagt atcttaaatg gaactgaaga ggattggaat aactacaaat
    14341 ggcaattatc aaatagaata acagatgtag atactttatc aaaaattata actctaacta
    14401 aaaaagaaaa agaatatata aaagaggttg gtactcaatt tagatgggca atatctccat
    14461 attatttgag tcttatagac ccagaagata tatgtgaccc aataaaatta ctgtctatac
    14521 caacacatat agagttggaa gatgagcaag aagatttgga cccaatggga gaagagtata
    14581 caaacccagc aggatgtata actagaagat acccggatag attaataata aatgtaacaa
    14641 atgagtgtgc tatgtattgt agacactgtc agagaagaag aaatattgga caacaagatt
    14701 ctcataagtc aaaagctatt atccaagaat ctatagacta tatcagagaa aatgaagaaa
    14761 taagagatgt actagtaact ggtggagatg ctcttacatt aaaagatgat tatttagagt
    14821 ggattcttag ccaacttaaa gagataccac atgttgatta tgttagatta ggtactagga
    14881 ctcttgttac aatgccacaa agaattacag atgaattttg caatatgcta aaaaaatatc
    14941 accctatata tataaatact cattttaatc atccaatgga aataactaag gaatctaaag
    15001 aagcttgtga aaagttagca aatgcaggcg ttccattagg aaatcaggca gtattattaa
    15061 atggaataaa taatgataaa tttgtaatga gatgtttaaa tcaagaatta ctgaaaataa
    15121 gagtaaaacc ttattatata ttccaaagta aacatgtaaa gggaacaaaa catttcaata
    15181 catcagtaga tgatggtctt gaaatcatgg agtatttaag aggatataca tcaggaatgg
    15241 ctataccaac atatatagta aatgctccaa aaggaggagg aaagactcct ttgcttcctc
    15301 aataccttgt atcaaaagga acagattacg ttatgcttag aacatgggaa ggaaaagtta
    15361 taaaaatgga agatgaacct gctgtagata taaagaaact tataaaagaa caagcacagg
    15421 attaaaaaaa tatttaaagt ttataaaaag aaacaacttt aaaataaaaa gaaaatggat
    15481 gtcaaattta tatcaatttg catccatttt ttattagtta tttattattt tgttgttgat
    15541 taactaattc aagatatcca cttgggtgga agtaatatat ctcagaagta ttttcattta
    15601 caaagtaacc aatatcacca tctgcatctg gtaaaaatac ataaccatta agatttttag
    15661 cttttaatgc ttcaaaatca ctagcatttc cttgatatat gtaatcaact tcattgttgt
    15721 attttactaa aagttcttta gcttgttctt gagtcaattt agtattttgg cttacaggaa
    15781 cattcttttg agaatctgca taagaagatt catttactgg tgcagttaat aacaaacaag
    15841 ataaaactaa taaagatata agtgcttttt tcatactaaa accctctttc atgtttaatt
    15901 atataagatt cattacttaa taatattgta tgtttcctat aattaaatat taccattaaa
    15961 gagaataata ttacagttac acagctgtaa cttttgatta caaaatcgta attaaattgt
    16021 atttttattc catttgtatt ttatcatgtc tacgatttac aaactcacag aagtatatct
    16081 ttataacgcc catcacagga gcagatagaa tcataccagg tataccaaag aatcctccac
    16141 caacagtaac tgctaggatt gtaaagaatg ggctaagacc tagttgtcca cctacgattt
    16201 ttggctctat aaatgtaact tcaacttgtt gaattattat taagtaaatt aaagaagtta
    16261 aagcaattgt tggattggag aataaattaa ttattacaac tggtgccata cctattactg
    16321 gaccaaaata tggaaccata ttcatgacac ccataagtgt accaaataat agtgcatatt
    16381 cagattttaa aaagtaaaga ccaattccag aaagtactcc aactataaaa gaatctaaaa
    16441 tcttacctgt aaagtattta ccaatattta aatttaagct ttgacatagt tctattataa
    16501 agtctccata ttttttacct aaggaaatat aaactacttt ttttgtaaac agtaagaatt
    16561 tttctttttc taataatata tagaaacaaa ttataaaacc taaaagaaat tgtactataa
    16621 attttccaac tgaaaatgta gtgctaaata tcccaccaag tgaaccaata agcaagttac
    16681 taaatttagg cattacactc ataattttgt caccatactc ttttaatgta ttagggtcaa
    16741 ctgattgtaa actcttgcct aagtcgaata aaaactgctc tgttttatct atgtatgtag
    16801 gaatttgagc aactaaatca gctaaactat ttactatgga tggaactgta aataatataa
    16861 acgaaattag tataactatg agtacaccat aagtaagaag taacgaatat attctcttaa
    16921 aatttaattt actttctaag aactttacta gtgggttgaa tatatatgct aatacaaatg
    16981 ctattataaa tggtgttagt agtgacatta ataaacttat cactccaaaa aaatatttgt
    17041 aattatcaat aactttaatc aagatatatg aaacaactac agtgagtaca atatttagat
    17101 aaattttttt atctt
//
LOCUS       DAELNY010000073        16670 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.073, whole genome shotgun sequence.
ACCESSION   DAELNY010000073 DAELNY010000000
VERSION     DAELNY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.073"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..243
                     /locus_tag="KPE55_002031"
     CDS             67..243
                     /locus_tag="KPE55_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493096.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            417..1547
                     /locus_tag="KPE55_002032"
     CDS             417..1547
                     /locus_tag="KPE55_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0493097.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKSYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1788..2138
                     /locus_tag="KPE55_002033"
     CDS             1788..2138
                     /locus_tag="KPE55_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0493098.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2146..2595
                     /locus_tag="KPE55_002034"
     CDS             2146..2595
                     /locus_tag="KPE55_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG0493099.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2697..3272
                     /locus_tag="KPE55_002035"
     CDS             2697..3272
                     /locus_tag="KPE55_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0493100.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3265..4674
                     /locus_tag="KPE55_002036"
     CDS             3265..4674
                     /locus_tag="KPE55_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG0493101.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4858..6291
                     /gene="eutA"
                     /locus_tag="KPE55_002037"
     CDS             4858..6291
                     /gene="eutA"
                     /locus_tag="KPE55_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG0493102.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6309..7673
                     /locus_tag="KPE55_002038"
     CDS             6309..7673
                     /locus_tag="KPE55_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG0493103.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7686..8567
                     /gene="eutC"
                     /locus_tag="KPE55_002039"
     CDS             7686..8567
                     /gene="eutC"
                     /locus_tag="KPE55_002039"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG0493104.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNAYI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKGNNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8587..9240
                     /gene="eutL"
                     /locus_tag="KPE55_002040"
     CDS             8587..9240
                     /gene="eutL"
                     /locus_tag="KPE55_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG0493105.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9251..9952
                     /locus_tag="KPE55_002041"
     CDS             9251..9952
                     /locus_tag="KPE55_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0493106.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGNTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            9955..11424
                     /locus_tag="KPE55_002042"
     CDS             9955..11424
                     /locus_tag="KPE55_002042"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG0493107.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKSVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11520..11807
                     /gene="eutM"
                     /locus_tag="KPE55_002043"
     CDS             11520..11807
                     /gene="eutM"
                     /locus_tag="KPE55_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG0493108.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11934..12695
                     /locus_tag="KPE55_002044"
     CDS             11934..12695
                     /locus_tag="KPE55_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HBG0493109.1"
                     /translation="MGVLTESEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12708..13337
                     /locus_tag="KPE55_002045"
     CDS             12708..13337
                     /locus_tag="KPE55_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0493110.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13379..14104
                     /locus_tag="KPE55_002046"
     CDS             13379..14104
                     /locus_tag="KPE55_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG0493111.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLISVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            14117..14389
                     /locus_tag="KPE55_002047"
     CDS             14117..14389
                     /locus_tag="KPE55_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG0493112.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14382..14933
                     /locus_tag="KPE55_002048"
     CDS             14382..14933
                     /locus_tag="KPE55_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0493113.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14945..16036
                     /gene="eutH"
                     /locus_tag="KPE55_002049"
     CDS             14945..16036
                     /gene="eutH"
                     /locus_tag="KPE55_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0493114.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            16038..16511
                     /locus_tag="KPE55_002050"
     CDS             16038..16511
                     /locus_tag="KPE55_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG0493115.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
BASE COUNT         6874 a         1873 c         3108 g         4815 t
ORIGIN      
        1 taaatatttt aaaagataat ttttttgaac taataggtat aacaaaaatt tataggggga
       61 ttgcatatgg aaatcaatgt tatagaaatt ttccctaaag ataaagctaa acttaataaa
      121 atagaaatgg ataaagctag ttggtttgta aatataataa gtaaaaaata tcctaaagaa
      181 gctttaaatg aagcatttag tactttagaa aaagaattaa atataagtaa agctaataca
      241 taagctttga ttattgattt tatatactat tggttttata cgctatgact tgacattata
      301 ttaatcaaat agtatatttt taaatatagc acaatgaagt gttaaaagag caatggagct
      361 ccatagataa agtctatgga gcttttttgc gtatttaact taaggaggat aggactttga
      421 ataactttga gattaaaact agaataaaat ttggagaagg ctcactaaaa gctttgcaag
      481 aattaaaaaa caaaaatgta ctaataataa cagacccttt tatggtgaaa tcaaaaacaa
      541 tagataaaat aacatctaat ttaataaact caacatatca agtttttgaa gaaatagtac
      601 cagacccacc aatagaagta gtagtagcag gaatagaagc atttaaatct ataaatccag
      661 acgtaataat agcattaggt ggcggttctg caatagatgc agcaaaatct ataatggact
      721 tttctaaaaa aatattaaaa attaacgaag tagagtttat agcaatacca acaacaagtg
      781 gaacaggttc agaagtaaca tcatttgctg taataacaga tagtcaaaaa aaatgtaagt
      841 atccattagt gagtgatgat ttacttccag atatggcaat actagaccca gagcttgtaa
      901 aaactgtacc aaattttata acagcagata cagggatgga tgtattgaca catgctatag
      961 aagcttacgt atctacaaat tctaatgact tttcagatgc actagctgaa aaagctataa
     1021 agttagtatt taaatactta ataaaatcat ataaagatgg aaatgacata gaagctagag
     1081 aaaaaatgca caatgcatct tgtttagcag gtatggcatt taaccaagca tcacttggca
     1141 taaatcattc tattgcacat gtattaggtg gaaaattcca tataccacat ggaagaacaa
     1201 actctatatt acttcctcat gtaatagaat ataattcatc aatagtagga tatactaaca
     1261 caacttactc tgatacagct aaaaaatatg cacaaatagc agaattagta ggacttggga
     1321 gtgctaatat aagaatatct gttagaaatt tagtaaatga aataaagaaa ctacaaaaag
     1381 aaatgaatat gccaactaaa ttgacacaat gcaaattaag tttagaagat ataactaatg
     1441 aagaaagaga aatagcaaaa cttgctataa aagatacttg tacattaact aatcctaagg
     1501 taccaactga agatgatata ataaacatat taaagaacat taaataacta cattttatta
     1561 catgtaaata taatattata caaataggga aaatgttttt aaaaatataa attatatatt
     1621 gacaaatata aaacataaaa ctataatgaa agtatcacaa aggcgtgata ctttagtaga
     1681 aagcaatgga gcttcttagg aatattaata ttattcttat ggagctcctt ttattttaat
     1741 caaatagctt tttaataaac tatattcaaa gaaagcaggg aataaatttg actgaagaat
     1801 ctaagcaaag agttattcaa gaatatgttc ctggaaaaca ggttacatta gcacatatca
     1861 tagctaaccc taatgaagac atatataaga agttaggatt agtacttgat aaaaaagatg
     1921 caataggaat acttacaata acaccaagtg aagcgtcaat aatagcagca gatgttgcta
     1981 caaaggcttc aaatgtatct ttaggtttta tagatagatt tagtggttca gtagtaatat
     2041 caggtgatgt aagttctgta gaatcagcgt taaatgatgt tttagaagtc ttaggaaata
     2101 tgttaaactt ctcatctaca aaaatcacaa ggacataata atacaatgaa gaatataata
     2161 tttatgggaa agacaggtag tggtaaaact accttatgtc aaaaactaga ccagttagaa
     2221 ttaaagtata aaaaaactca atcagtggaa ctttataata atgcaataga cactcctggt
     2281 gagtatatgg aaaatagaaa cctatataat gccttaataa caacatcagc agatgctaaa
     2341 attatagcaa tagtgtatga tgccacacaa gaagaaaatt atatagcacc aggttttgcc
     2401 agtatgtttt gcaaagacgt aataggaatt ataactaaaa taaataaagc tactgaaaac
     2461 gaagtcaata tagcttatga aagactatca ctagcaggag caagtcaaat ctttaaaata
     2521 gacacagttg ataatattgg acttgaaaaa ttatttaaat atctagaaaa atataaaaat
     2581 atagatgaag tttaagggga aatatttttt tattcaaaaa tattattaaa gtatgataaa
     2641 ataaaaaaat gtataaacta ttttaagaag atttaatctt taagcgaggt aggaacatgg
     2701 ggaaaaaaat actattggtt gatgatgaac cactgttaag gatggataca aaagatatat
     2761 tagaaagtca tggctatgaa ataataggcg aagcaagtga tggatttgaa gctgttgaaa
     2821 tgtgtaaaaa acacaaacct gatttagtta taatggatat agagatgcca atacttgatg
     2881 gtataaaggc aggcaaaata atatgtaaag aaaatctagc tggtggagta cttttattaa
     2941 cttcatttga agataaagaa tacgtagaaa aggctaaaag tataggtgct tatggatatt
     3001 tagtaaaaac agcaagtgaa aaatcactta tcccaaccat agaaatgtgt ttaaataagg
     3061 taaatgaatt tgaaaaaatg aaaaaagatt tagaaaaagt aaattctaaa ttgaatgaaa
     3121 gaaaattagt agaaagagca aaaggtatac ttataagaga gtttaagatt tctgaggatg
     3181 aagcatacac tagaataaga aaattaagta tggataaaag aactacaatg atggaaattg
     3241 ccaaaatgat tataatagga tacgatgact aatacgataa aaaaaatatc aactgcacaa
     3301 acatacttaa ataataacga aattgaaaaa attatagaag tatcaaaatc cttagattta
     3361 atggcttctt tttatgaagc tgatgttttt atagatgtac taagtaaatt tgatgatgaa
     3421 gcaattgttg tggcacatgg tagacctaaa gaaaagtcaa tgtactgtga aaatgttata
     3481 ggtaaaaagg ctttaattga aaatgaacca ggagttataa agtgcttaaa aacaggagaa
     3541 agagtaagag atattaaagc tttaacacag gaatataaac tggtcaaaca aaatattcag
     3601 ccaataacac tagatgacaa agttttagca gtactaatag ttgaaaaaga tataagtagt
     3661 gaagttaaga atgagtttaa tgctagtgaa gacgaagaaa ataaaaattt acatcagttt
     3721 ataaaactta ttggaaataa tgacttttta gttgataatt taaattcagc catattaata
     3781 tttgataaaa atggaaaatt aaaaattaaa aatcgtattg caactgagat atacaaaaat
     3841 cttgggtttg gatatgatat acaagatatg cattacaatg atttagatat agaaaccaac
     3901 acctttgaaa atatattaat ggatgaaaat tatgatgaga caaatgaagt ttcaataggt
     3961 aatatgtttt ttaaaataaa gaccatatac atcaaagatg atgaatttaa ggtttgcaaa
     4021 atagtacaag atataagtga tttaaagaga aaagaagaag aattagtttt gaaaacagtg
     4081 gctataagag aagctcatca tagagtaaaa aataatcttc atacagtaat atctataata
     4141 aaaaaacaaa gtagattatc tcaaaatgat gaagtaaagc agtgtttaga taacataata
     4201 aatagggtat ttgcaatact ttcgactcat tatttactat ctaaagaagt tgataataat
     4261 atatctatac gagaaggtat caatttatta gtgtcaaata ttcaaggtgg ctatttattt
     4321 agtaaggata tagatatacg tataactggt gaagatttta aaattagtgg agaaaaagca
     4381 acagcaatac ttttggttat aaatgaaata ctacaaaatt gttatgacca tgcattttcg
     4441 gatagagaac atggtactat acaaatatta ataaataaag ataacgacta tagaaatatt
     4501 gctattatag atgatggaat tggatttgat agtaataata taaataaaaa tagcttaggg
     4561 acatatataa tagatagtta tataacacag atgttaaaag gaaatataga gagaaaaagt
     4621 agtgagaatg gtacagaagt attaattact atacctacac aaattaatat ttaaataata
     4681 actcatctac aaagtagtag ttttaattga gcaaagaggc ttaaggttat taatagatta
     4741 agttaattga atgtatttat aaaaattata agtatatatt caatttaaca ggtatttatt
     4801 ataactttaa gcctctttgc ttgtttaaga catacttaaa ttttaggagg tgagaaaatg
     4861 agagaagagt tattaagtgt tggtattgac attggaacaa gtactactca gttaattttt
     4921 tctaaattaa tagtagaaaa tactgaatca aatttttcgg tacctagaat aagtatagta
     4981 gacaaagaaa ttatttataa aagcaaagtt tatttcaccc ctcttatttc aaataatgaa
     5041 ataaatggtc aacaaataaa agatatagtt gaaaatgaat ataaaaaagc aaatatatct
     5101 aaagaagata tacatacagg agcagtaata ataactggtg aaacagctag aaaagaaaat
     5161 gcaaatgatg tacttcatac tttgagtgga tttgctgggg acttcgttgt tgcaacagca
     5221 ggacctgatt tagaaagtat aatatcagga aaaggagctg gagctcatat ttattcaaaa
     5281 gaacattcaa ctacaattgt aaatatagac ataggtgggg gaactagtaa catagcctca
     5341 tttaaaaggg gagaagtaac cgatactgga tgtttggata taggtggaag gcttgtaaag
     5401 atagataaaa taagtaaaaa aataacttat atatcaccta aaatagataa aatcataaaa
     5461 gataataatt taaatcttca agtaggcaca atggctactg tagaaaatac aaagccaata
     5521 gtggatataa tggttgactt attaatggaa agcataggat taaagaaaca gacacctttg
     5581 tttgatttta taataaccaa taaatcaata agtatagatg aaactattaa aaatgtttca
     5641 ttctctggtg gagtagctga ttatatttac tatgatggag aaataaaaga ttattttgaa
     5701 tatggtgact tgggaatttt acttgggcaa gcaataaaaa attgtgaata ccttaaaaaa
     5761 gtaaatgtag ttaaatctat ggaaacaata agagccacag tggttggagc aggttctcat
     5821 actactgaag taagtggaag tacaataact tacactagcg aaaagtttcc acttaagaac
     5881 ttgccagttt taaaattatc aattgatgaa gaaacaggta cttcaaaaca aatcagtgaa
     5941 gctataaaaa ataaacttaa ttggtttaaa ctagaaaatg atttacagaa agttgctata
     6001 gccatgatag gaaagaaaaa tccaaccttt ttagatatac aagagtatgc taaagggttt
     6061 gtagatggaa tgcaacaaat catagaaaat gatttacctt ttatagtaat tgttgaaaat
     6121 gatatggcaa aagtgttagg tcaagcaata tatgcattat taaattatga aaaagaaatt
     6181 gtttgtatag acagtgtcaa ggttgaaaat ggagactata tcgacattgg aaaacctata
     6241 gttgaaggta aagttttacc tgtggtaata aaaacattag tttttaatta atagaaggag
     6301 gtaagtaaat gattttaaat acaaaattat ttggacgtac ttatcagttt aaaggcttat
     6361 atgatgtaat ggctaaagct aatgaagaaa aatcaggaga taaattagca ggtcttgcag
     6421 cagaatcagc tgaagaaaga atagcagcaa aagttgtttt atcacacata aaattggagg
     6481 atataagaaa taatcctgca gtggcatatg aagaagatga agtaacaaga ataatacaag
     6541 atgctgtaaa tgaaaaagtt tatgaagaaa taaaaaattg gacagtctct gaacttagag
     6601 aatggctatt ggattcaaat actacaagtc ttgatataag aagaatatca agaggattaa
     6661 cttctgaaat gatagcagca gttgcaaaat taatgtcaaa tatggactta gtttatgcag
     6721 ctaaaaagat aaaggttact gctcactgta atacaactat aggagaacaa ggaacattat
     6781 cagcaagact tcaacctaat catccaacag acgacccaga tggaatactt gcatcacttc
     6841 ttgaaggttt aacttttggt ataggagatg cagtacttgg attaaatcca gttgatgact
     6901 ctgttgagag tgttactaaa gtattaaaaa gatttgaaga agtaaaacaa aaatatcaaa
     6961 taccaactca aacatgtgta ttggcacacg ttactacaca aatggaagca ataagaaatg
     7021 gtgctcctgc agatttaata ttccaatcta tagcaggttc tgaaaaagga aatgaagcat
     7081 ttggatttaa cggcgacact atagaagaag ctagacagtt agctttaaaa cacggtacag
     7141 ctacaggacc taacgttatg tattttgaaa ctggacaagg ttcagaatta tcttcagaag
     7201 cacatcatgg aatagaccaa gttactatgg aagctagatg ctatggattt gctaaaaagt
     7261 atgaaccatt cttagtaaat acagttgttg ggtttatagg acctgagtac ttatatgatg
     7321 ctagagaagt tgtaagagca ggacttgaag accactttat gggtaaacta catggcttac
     7381 caatgggtgt tgatgtttgt tacactaacc atatgaaggc tgaccaaaat gatatggaaa
     7441 acttagccat actattaaca acagcaggat gtacgtattt tatgggaata cctcatggag
     7501 atgatgttat gcttaactat caaacaacag gataccatga aacagcatca cttagagaaa
     7561 tgtttggact aacagcaata aaagaatttg atgcttggat ggaaaagatg ggattctcta
     7621 aagatggtaa gttaacagag ttggcgggag acgcatctgt attattagga taggaggtat
     7681 ttaatatgaa tgagaaggat ttaaaagcat tggtagaaca attagttgga caaatggttg
     7741 gagaattaga tactaatgtt gtgtctgaga ctgttaaaaa ggcaactgaa gttgtagtag
     7801 acaacaatgc ttatatagat gatataacag aagttgatat aagaaagcaa ttactagtaa
     7861 aaaatcctaa ggatgcagaa gcttatttag atatgaaagc aaaaacacct gcaagattag
     7921 gtataggaag agctggtact agatataaaa cagaaacagt tttaagattt agagcagacc
     7981 atgctgctgc acaagatgct gtattctctt atgtagatga agaatttata aaaggaaata
     8041 atatgtttgc agtggaaact ttatgtaaag ataaagatga atacttaaca agaccagatt
     8101 tgggaagaaa attttctcca gaaactataa ataatataaa atctaaattt ggtacaaatc
     8161 aaaaggtttt aatattagtt ggagatgggc ttagctcagc tgcaatagag gcaaatttaa
     8221 aagactgtgt gccagctata aaacagggtc taaaaatgta tggtatagat tcaagtgaaa
     8281 ttttatttgt taaacattgt agagttggtg ctatggacca cttaggtgaa gaattaggtt
     8341 gtgaagttat atgtatgttg gttggagaaa gaccaggatt agttactgct gaatcaatgt
     8401 cagcatatat agcttataag ccatatatag gaatggcaga agcaaaaaga acagttatat
     8461 caaatattca caaaggtgga acaactgcag ttgaagcagg tgctcatata gctgaactta
     8521 taaaaactat gttagataaa aaagcttcag gaatagacct taaataaagt cgagggagtg
     8581 tagaatatga aaaatgatgt aattcgtcct aatatactag gaattaaaat aatttcaaat
     8641 ataagtccag aaatggcaca aaaactagaa ttaaaatcac atcacaaaag cttaggttta
     8701 ataactgcag actgtgatga tgttacgtat acagctcttg acgaagcaac taaagcttca
     8761 gaagttgatg ttgtatatgc aaaatcaatg tatgcaggtg ctgcaaatgc ttcaacaaaa
     8821 cttgctggtg aagtattagg aataatagca ggtccaagcc cagcagaagt aagaagtggt
     8881 ctaaatgcag tagttgattt tctagagtat ggagcaactt ttataagtgc aaatgatgat
     8941 gattcaatag catactatgc tcactgcgta tcaagaacag gtacatacct ttcagaggta
     9001 gcaggaataa gagaaggtga agcattagct tatttagtag caccacccct tgaagcaatg
     9061 tatgcactag atgcagcaat gaaagctgca gatgttaaga tgtgtgaatt atttgcacct
     9121 ccaactgaaa caaactttgg tggagcatta cttacaggtt ctcaatcggc ttgtaaagca
     9181 gcatgtgatg catttgcaga agcagtaaaa tcagttgcag acaatccaac aggattttaa
     9241 ggatagttag atggatgcta taggcttaat agaagtaata ggatatgttg ctgcaataga
     9301 agcttctgat gcttgtttaa aatctgcaaa tgttaatatt gtaagagttg ataaggttgg
     9361 ggcaggtatt gttactttaa caatatgtgg agatgtcggt gctgtaaaag ctgctttaga
     9421 agcaggtgaa atagcagctt ccagagttgg tacattaaga acatcacata ttataccaag
     9481 aatccataat gaagtaacaa atgctttatt caaaactaaa gaacctaaag tctgtgaagt
     9541 ttcagaagat aaagaaaaaa actttgagat tacattggaa ataactgata atgtatcgaa
     9601 aaatttatca gaaaaagaaa atgtaatttt agatgtcaat gaaaatagcg gaaatactga
     9661 aaatttatat agtgatatag ataaaacaaa caaaaaaatt gaaacagttg atgatagttt
     9721 aacagaagct aatgaggaaa aaatagaaaa cactaatgat aaagaagtat tggaacaagt
     9781 taaaatagaa aattccgatt ttgatagaga tgacctatca aagaacagtg taaaaaaact
     9841 taaagctatg gctaagaaat tagattcttc aataacttat aaagatttaa atgttcttaa
     9901 aaaagaagaa ttaataaact tagttaaaaa gcttagcaga agggatagat agttatgaca
     9961 cttttagata aagatttagt atctatacaa gaagtcagaa ctcttgtaag aagatctaaa
    10021 gtggctcaag agaaactttc taaaatgagt caagagcaaa tagataaaat agtaaaatct
    10081 gttgtagatg cagcatttaa taatgctact aaattagcta aaatggcaaa tgaagaaact
    10141 ggatttggaa aatgggaaga taaagtaatt aaaaatactt ttgcatctat gggagtttat
    10201 gaaactttaa agaacacaaa aactattgga ataattggtg aagataaaga agccaatacg
    10261 atagatatag ctacaccagt tggagttgtt gcaggtctta taccttcaac aaacccaaca
    10321 tcaacagtaa tatacaaaac tataatatct ctaaaagcag gaaatactat agttttctct
    10381 ccacatccaa atgcaaaaaa ctgcatactt gaaacagtta atataataag tgaggctgct
    10441 ataaaggctg gagctccaga ggattcaata ttatgtataa ctaatccaag tatagttgga
    10501 accaatgaat tgatgaaaca taaagataca aacttaatac tagcaactgg aggagaagca
    10561 atggttagag ctgcttactc ttcaggaaca ccagctattg gagttggacc aggaaatgga
    10621 ccagcattta tagataaatc agcaaatgtt aaattagcaa tcaaaagaat aatagattct
    10681 aaaacttttg ataatggtgt tatatgtgca tcagaacaat ctatagtaat agaaaaatct
    10741 atggaagaaa ctgttttaaa agagcttaaa aatcaaggtg catatgtttt aaatgaagag
    10801 gaaagtgaaa gattagcaaa attcatatta agagctaatg gtactatgaa tccacaaata
    10861 gttggtaaaa gtgttgaggt aatagcaaat ttagcagact taacaaatgt acctaaaaat
    10921 acaaaagttc tagttgcaag agaaaataaa gttggtcaca atgtagccta ttcaagagaa
    10981 aaacttacac caatactagc gttctatgtt gaagaaaatg tagaagctat tatgaacaga
    11041 tgcagagaaa tattgttaaa tgaaggtgca ggtcatacat tctcaatgca tgtaaatgat
    11101 gaagaattgg taaaaagatt tgcccttaat atgccagtat caagaatact tgtgaatact
    11161 ccttcagcac ttggaggaat aggtgcatca acaaaccttg taccagcact aacacttggt
    11221 tgtggtgcaa taggtggaag ttcaacttca aacaatatag gaccacttga tttaataaat
    11281 gttaaaagag tagcttatgg aatcaaagaa atagaagaac ttggaagaga taaatctcaa
    11341 gttgaaaaat caatgtttga ctcaatggat aaagaagaat taataaatct tcttgttcaa
    11401 aaactagtca aagaactagc ataactaaaa taaacaatga aacttatagc tatcactata
    11461 aatttctaaa aatataaact ataaaaaact ataaaattaa atttttggag gataagatta
    11521 tggcaagtgc aaacgcatta ggaatgatag aaactaaagg attagtagga gcaatagaag
    11581 cagcagatgc aatggttaaa gctgcaaatg tacaattagt aggaaaagag caagtaggtg
    11641 gaggattagt tactgtaatg gtaagaggag atgttggagc tgttaaagct gctacagatg
    11701 caggagctgc tgctgccgag agagttggag aactaatatc agttcatgtt ataccaagac
    11761 cacactttga agtagatgct atattaccaa aggtatcagc tgagtaatat aagaataatt
    11821 ataaaattta aattttgatt gaaaaagagg ctatctcaaa aatgagtctt actaataaac
    11881 tcaatgtatt ttgaggtagt cttttttata catctaatag gagatgtgat aaaatgggtg
    11941 ttttaacaga aagcgaagta aaaagaaaaa ttggaaaaaa caaaaataat ataactgaat
    12001 tttatataga aaaaggtcaa ataataactc cttctgcaaa atcttattta gctgagagaa
    12061 gtattgagct taaatatgtt gataaacttt cagatgtaaa caatgaacct aaagaagttg
    12121 taaaagaagt agttaaagag aaaaaatata gatatactac tgtatttggc gctaaattgg
    12181 atgaaaaacc agaacatatg actcaattaa atggaaatct tttagttttt aaagaccata
    12241 aaagaataat attgagagga aaaatagatt ctcttgaatc agaaatacta aaggtacaga
    12301 tactttgtca gaaactagat atgccaaagc ttgttaagga attgcaagaa gtattagact
    12361 ttgtaagaaa tataatgaga tgtgaagttt tagatgaaaa gctaggggac tttaaacttt
    12421 taggattaac tcctcaagaa cttagagaac aatctcacta tcctaaaaaa tactttggta
    12481 taggacatga gttcccaagt tatgaaatgg gagaagtagt agtagctata aatagtataa
    12541 gaagtaatac acgagaggtt gaacttgcag cctatgaagc atttaagggt gagtatggaa
    12601 atgttcacag agaagattta ataaaggcac ttaatagatt atcgtcagta ttttggataa
    12661 tgatatataa ggttagaact aataagtata agtagttgga ggacataatg gagaatctat
    12721 taaatgaaat agtggaagaa gtaataacta gagttaaaaa agaagcattt atagaagttg
    12781 aagcatcagg aagacacgtt cacttaagta gagaagatgt cgataaatta tttggagaag
    12841 gatatactct tacaaaacta aaagacctat ctcaaccagg tcaatatgca tgtaaggaaa
    12901 gagttacaat aactggtcct aaaggaagta taaaaaatgt agttgtactt ggaccatgta
    12961 gaaatgaaac tcaggtagaa atatctttaa cagatggttc aactcttgga ctaaaagctc
    13021 ctataaaaca aagtggagac ttagaaggta ctttaagtat taaaatatca acacaatatg
    13081 gtgaagttga acttgataag gggttaatgg ttgctaaaag acatatacac atgactccta
    13141 aagatgctga aaaattcaat gtatgtgata aagagatagt tgaagctaag gtgatgggtc
    13201 aaagaccttt aacatttgat gatgtagtaa taagagtgag tgacagtttt aaaacttata
    13261 tgcatataga ctatgatgaa gccaatgcat gtggttattc aaaaggaact gttgctaaaa
    13321 taataaagaa agcgtaaatt taacctacta tataaattac ctctagggag gatttaatat
    13381 gaattatgat aaccttgtag atttaataac ggaagaaatc tataaaaaaa taaatagtaa
    13441 tgaaataaaa atatctaata aacctaaagc tgtgattgta tttgaacaag ataacaataa
    13501 atttaactta ttaaaagatg aatttgaaat agtagaattt gataaaagta taagggaatg
    13561 tgaaatagtg atagtttcaa gactttgtat gagggggcta tcgaatatag cacttggaaa
    13621 ttcaacaagt gatgaagaaa gatttatact aaaaatgatt atgaagggca aagaagtata
    13681 tgtcctagat gaaggaattg aatacaaaaa atataaggat actgcaccaa aagcattata
    13741 taagaaattt atgtcatttg aagatgaaat atgtaaattt ggtgtagaaa ttataaaaga
    13801 cttaaatgtt ataactaaaa ataagttaaa tataaagaaa gaactagaaa gtactagagg
    13861 ttctaaaaaa gaagatacaa aagttttaga taaagatgtt ttaaatttaa tcagtgtaga
    13921 aagtgtaaaa atagataatg aatttagttt agatttaagg aataaaaaac ttatatcgga
    13981 agctgactta agaaaaccaa ctataaatgg agttaaaaac atattagtta ataaaaaaag
    14041 tataataaca ccattagctg tagattttat gagaatacac catctcaaac taaagaaagt
    14101 gtaaggaggt aattttatgg atataggaaa agttgtaggt aatgtttggg ctacaagaaa
    14161 agatgaaaaa ttatgtggcc aaaaactttt agttgtaaaa attttaaaaa ctaaagacac
    14221 atacaaggaa ggcttcatgg ttgcagccga taatgcagga gcaggaaatg gagacttagt
    14281 tcttatatcc aaaggttcct cagctagaga gtcgattgaa aaatctcatg ttccggtaga
    14341 tgcaactata gttggagtag tcgattcgtt tgaggtgttt gatgaataaa agtataggag
    14401 ctatagagtt taaaagcatt gctaaaggta tagaagtatc caatgaaatg ataaagaaat
    14461 cttcagttga tgtattgtac ttaaaaagta tttgcccagg taaatttcta ataatagtag
    14521 gtggagaaac ttcttatata aatgaatgtg tagattatgg aataaaactt ggagaaggat
    14581 atattgtaga taacttcgta ataaatgcaa tttcacaaga aatattagat ggatttaaaa
    14641 ataagtatca aaagctagat agtatagtct ctataggcgt tgttgaaagt agtaaagtat
    14701 gcgctggaat aaaaatgctt gataagactt taaaatctgg cgacttagta ttagtaaaac
    14761 ttcaattatc ttttgctata ggtggcaaat tagtatatat agtagcagga gatttaagta
    14821 gtttagaata tgcattaaaa gaaagtgaaa atgtagtaag agaaaaagaa gtaatatata
    14881 agactgtaat accatcagta gacagtcaaa taataaaaag tctaataaaa taatgggggg
    14941 aattatgagt ataaacgaaa ttataatcta tgtaatggta gtcttcatgg tacttggagc
    15001 tatagataaa tgtataggta acaaatttgg tcttggagaa caatttgaag aaggtataat
    15061 ggcaatgggg tcacttgcag ttgcaatggt tggtgttata tgtttagcac cagttcttgc
    15121 tgatgtatta agaccaattg tagtaccagt atttaatata cttggagctg acccagctat
    15181 gtttgctgga agtttattag ctaatgatat gggtggtgca ccacttgcac ttgaacttgc
    15241 gaaagaccct aatgctgggt tatttggtgg attaatagta ggggctatga tgggacctac
    15301 aatagtattt ataataccag ttgcgcttgg aataattgaa aaagaagacc aaaaattctt
    15361 agcaacagga atacttgcag gtataataac aatacctatt ggagcattcg ttggtggatt
    15421 agttgcagga ttcgaggtaa tgatggttct tagaaactta gtgccaataa taatatttgc
    15481 agtagttata gcacttggat tattaaaatt cgaaaatgct atgataaaag gattcacata
    15541 ctttggaaaa ggtgtagtaa tagttataac tttaggactt gctgctgcca tagttgaagc
    15601 tttaacagga atagttgtaa tacctggaat gaaaccaatc agtgaaggta tagaaatagt
    15661 tggagatata gcaatagttt tagcaggagc attcccatta gtgtttgtta taacaaaggt
    15721 atttaataaa ccattaatgg ctttaggaaa agttcttgga atgaatgatg tatcagcagc
    15781 aggccttgtt gcaagtttag ctaactgtat accaatgttt ggtatgatga aagatatgga
    15841 caatagagga aaaataataa atgtagcatt ctctgtatca gcagcattcg tatttggaga
    15901 ccacttagga tttacggcag gatttaattc taatatgata acaccaatga tagttgctaa
    15961 attagttggt ggtataacag cagtattgtt agctatggtt atcgcaaata agacactaaa
    16021 gaaggagaat gtataagatg gatatatcaa atatagataa aaatttaatt gaaacattag
    16081 taagacaaat aatagaagaa aaaataagtg gaactaaaga cacagtagat tttgttagaa
    16141 ataaagacat tagtggaata acttctataa agttaccaac tgtaaaagtt agtgaatctg
    16201 atagattaga tacagggaat ccaagtgatg tagtttacac aaaagattta tttacattag
    16261 aggaaagccc aagattaggt tgtggtatga tggaaatgaa agaaacaact tttgattgga
    16321 ctctaaacta tgatgaaata gattatgtaa tagatggaac tttagatata ataatagatg
    16381 gaagaaaggt ttctgcttct tcaggtgaat taatatttat accaaaagga agtaagatac
    16441 aattttctgt acctgattat gctagattta tatatgtaac atatccggca gattgggcat
    16501 ctcaaaatta ataaaaatca agttaagtta ataataatca aataaataat ttaaaataca
    16561 aactcttaaa taagcacaca tatttaaaaa agctatgaat taaaattcat agcttttttt
    16621 ataaagaatg ttatatttta gacgtatttt ttctatattt actgggagac
//
LOCUS       DAELNY010000074        16664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.074, whole genome shotgun sequence.
ACCESSION   DAELNY010000074 DAELNY010000000
VERSION     DAELNY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.074"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            395..838
                     /locus_tag="KPE55_002051"
     CDS             395..838
                     /locus_tag="KPE55_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0493116.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            996..1166
                     /locus_tag="KPE55_002052"
     CDS             996..1166
                     /locus_tag="KPE55_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493117.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            1539..2636
                     /locus_tag="KPE55_002053"
     CDS             1539..2636
                     /locus_tag="KPE55_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0493118.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            2841..3329
                     /locus_tag="KPE55_002054"
     CDS             2841..3329
                     /locus_tag="KPE55_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG0493119.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            3415..3822
                     /locus_tag="KPE55_002055"
     CDS             3415..3822
                     /locus_tag="KPE55_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493120.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            3823..4041
                     /locus_tag="KPE55_002056"
     CDS             3823..4041
                     /locus_tag="KPE55_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493121.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            4256..4417
                     /locus_tag="KPE55_002057"
     CDS             4256..4417
                     /locus_tag="KPE55_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493122.1"
                     /translation="MKNQANGLKNELIIGKNQAYRLENELIIGKNQAYRLENELIIGK
                     NQAYRLKNK"
     gene            4810..5115
                     /locus_tag="KPE55_002058"
     CDS             4810..5115
                     /locus_tag="KPE55_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493123.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            5523..6212
                     /locus_tag="KPE55_002059"
     CDS             5523..6212
                     /locus_tag="KPE55_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493124.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGCAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            6212..7129
                     /locus_tag="KPE55_002060"
     CDS             6212..7129
                     /locus_tag="KPE55_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493125.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKCINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            7202..8128
                     /locus_tag="KPE55_002061"
     CDS             7202..8128
                     /locus_tag="KPE55_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493126.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVEETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            8125..8805
                     /locus_tag="KPE55_002062"
     CDS             8125..8805
                     /locus_tag="KPE55_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493127.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIIFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            8903..9238
                     /locus_tag="KPE55_002063"
                     /pseudo
     CDS             8903..9238
                     /locus_tag="KPE55_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            10222..10872
                     /locus_tag="KPE55_002064"
     CDS             10222..10872
                     /locus_tag="KPE55_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0493128.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            11221..13287
                     /locus_tag="KPE55_002065"
     CDS             11221..13287
                     /locus_tag="KPE55_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0493129.1"
                     /translation="MKEVVGSLKKITKERTENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            13304..15259
                     /locus_tag="KPE55_002066"
     CDS             13304..15259
                     /locus_tag="KPE55_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0493130.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            15275..16465
                     /locus_tag="KPE55_002067"
     CDS             15275..16465
                     /locus_tag="KPE55_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0493131.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
BASE COUNT         6456 a         1746 c         2880 g         5582 t
ORIGIN      
        1 atattataga tgaatattaa tattgatatt ctaggtaatt gctagagctg atattataga
       61 tgaatattaa tattgatatt ctaggtaatt gctagagtta gtattataga tgaatattag
      121 tattgatatt ttaggtaatt gctagagctg atattataga tgaatattaa tattgatatt
      181 ctaggtaatt gctagagtta gtattataga tgaatattag tattgatatt ctaggtaatt
      241 gctagagctg atattataat ttattattag tattaatatt ctagggaatt atcactatat
      301 tttaaaaagt aaaaatatat atcaaaatga actaaataag tactgtaaga agtttgttaa
      361 attaaggttt gaataaagtg agggtggaga acttatgata gaaataaaaa ctatagaaat
      421 aaataaaaaa agtcaaattg accttttaat gttagccgac ccatgtgaag aaatgattga
      481 taaatatctc aataaaggaa ctatgtacgc attgtatgat aataaagaac ttacttgtat
      541 agcagttgta aatgaaattt ccaaagaaat atgtgaatta aaaaacattg ctacatatga
      601 gcattttcaa aatatgggtt atgcctcaaa gatgatttat catcttttag atgtttatag
      661 taaaaaatat tcatcaatgg ttgttggtac atctgaaagc ggagttcctt tttatgagaa
      721 gtttggattt gtttattcac ataaaattaa aaactttttt gtagataatt atccagaacc
      781 aatctttgaa ggagaattac aatgtgttga catgttgtat ctatcatata acttttagat
      841 aggttaatat aattaattaa ttcatataat tatcggaatt ataatttata aattatgtat
      901 aataagagta taatttattt agaagctgtt atattgtatt atattcaaat aagatatata
      961 ataacttgtt tattttgaat tttggaggat taattatgga ttcaaataat aatactataa
     1021 aatcaactgt taaaaagggt atttcttttg gttcttgttt agcaatgatt atttcttata
     1081 ctgcatggaa atctattcca tgggctattt ttcatggctt aatgagttgg atatatgtac
     1141 tttattattg ggttaagtat gcatagatta aattaataaa taagtatgta atattgtaaa
     1201 gtttaagaaa caaaatgtaa gggagtgtct cgaagtgaac ttttagttta taaggcactc
     1261 ttttttgtat taaataaaat atattttcat tatttaaaca atgaaaaaga tgagtatctc
     1321 tattttaata cataccctta tattcattca tttatcaaca atagttttaa aaagaaagct
     1381 atctaaaatc tattgtaatc taagtgcttc tctttaaagt cttaacttta gtaatgatat
     1441 ataagtttat aaggttctta tttcaataat gcaaatttta cttagaagtt cagactttgg
     1501 tatgaagaaa tgggtaataa taaaaaagag gtgttgatat gaaagaaaaa ttagaagaaa
     1561 aatctaagat tattaaaaca tcaaaagagt ataaacgaac tataattgaa gaaaaggaca
     1621 gagaatggac caaaaaatta aatgacatac ttagtagagg tgatttttct aatgaaaaat
     1681 tagataaaat caaggattta attccaaaag aaatattgac tagtgaaaat gttggagaag
     1741 ttgtaactga agatgggtat gctaagcctg agtatgatga ggtatttaaa tcattgttta
     1801 ctttaaataa tgattacgat ttacttgcaa attttataaa tgatatcctc aaagatgctc
     1861 catatgccaa taggaatatc aaacagttca ctcaaataaa aagaataatt aaggttgaaa
     1921 ccgacccaac aattaattac attggtgaga aaagacctag acttgatatt ttggctgaag
     1981 atgaagaacg taaccatata aatattgaga tgcaaagagc tcttgaagag gattatttgg
     2041 aaagagctga atattatctt agtcgtgtac atggtagaaa gttagaagaa ggtaaagaat
     2101 ataaagaaat tggaaaaact gttggaatac atatactaaa tcatgtaaaa tataatcaca
     2161 tagaagatta tgtaaattgc ctgagattaa caatggacgg gcatccagat attttttcta
     2221 gtaaaactgc attatatttt atagaacttc caaaaattcg taaatccagt tgtatagcaa
     2281 acagagttct aatatggggt aaacttattg ataatccttc tcatatagat attagaatac
     2341 tatctaaaac ggattatgtt attaaaagag cacttgatag attaaaagaa ttaggtagta
     2401 atgaggacta tttattaaat ttaaagagag gtgcgtatat tatgaataaa agtagaaatt
     2461 ttaaagaaga aatttttcaa gaaggtatcg aaaaaggtat tgcaaaaggg aaaagagaag
     2521 agaaatttaa tatcatcata aaattaaaaa ataaaggata caatttgagt gaaatatgcg
     2581 atattataga tgatttgaat aaatcagagg ttgaaaaagt ctataatcaa aactaatact
     2641 atgattgaga tggcactaga taaatacaaa gcttcctctt ttaaatttaa attaatataa
     2701 aactttgtta tttaaatatg gtagatataa agagatatat aaataatagg tacaaagagg
     2761 tgtataaata aaggagataa ttcaaaataa aagtttgaac catctccttc ttatagaaaa
     2821 acaaaggggt tgatttatat atgaaatttt atactataca gactttagaa ttttggaata
     2881 acaataaaaa caataaatat ctatcaaatg attattcttt tatattagaa gactttgata
     2941 ttccatacaa atggatgtat agtcaaatga caaatagaat atatgaattt gatgatagta
     3001 tgatatgggt ttggacgaag agacctgatt taagatgtag tggttattta gaaaaaggaa
     3061 aaaaaggtgt tttacttgaa atagaaataa gtgaagacaa tgtgttatta tcagaatttg
     3121 atttatggca tttaccttta atggatatta ctgtagagct ttatgaagat gaacaaattg
     3181 aaaagaaggt aagttgggaa agaatatttg acttagaact gtgttcaaaa ttaaatgaag
     3241 ttaatataga agatttgaca ttgcaaggag tgacatctaa agtgcctcta tctaatgtaa
     3301 aattaataaa agaatttgtt gcaaaataat atttttattg aaatttattc aaaaaagtta
     3361 tatatttata acaagaggtg ttataaatac ataacttttt aagggggatt ttatatgtct
     3421 aaacaacaaa ctggtattaa atcaacactt atttcagaaa tattgggaat ttcttattgt
     3481 attggagcta cattagtaaa taagtatttt tttaaaggaa gtagtattga atatcttcct
     3541 ataattattt gggtattagc tatgggagct cagtgggtct ataataaaaa gtatgatgat
     3601 ttaattgatg aggcatcaaa atgtatacta tcaagagtta atgatattgc aataaaagta
     3661 ttgttttttt ctgtagctat agtatctata tttcttgtaa ctttggacat aaatgtgtca
     3721 aatttaaata taggaatgtc tttattagtc attttgttta ttcaatcctt gttgaagtta
     3781 attctattta tatattttga cagaaagggg atatacaatt aggtgtcagt attactaaag
     3841 acaaaaatta aatcctatag agaaaagaac cttatgacac aaggtgaact tgcaaagtca
     3901 gtaggagtaa gacgtgaaac tattgtacat ttagaaaatg gtcgctacaa tccttcttta
     3961 aaactggcaa tggatattgc taaggtattt gatacaactg tagaagagtt atttgaattt
     4021 attgaagaaa ataatattta aatttcatgt attacttatg cattagagaa tgaattaatt
     4081 ataggtaaaa aaccaagctt atagattaga gaataaatga cttatagatg aaaaaccaag
     4141 ctaatggatt aaagaatgaa tgatttatag atgaaaaaga aagcttatag attagagaat
     4201 aaatgattta taggtaaaaa ccaagcttat agattagaga ataaatgact tatagatgaa
     4261 aaaccaagct aatggattaa agaatgaatt aattataggt aaaaaccaag cttatagatt
     4321 agagaatgaa ttaattatag gtaaaaacca agcttataga ttagagaatg aattaattat
     4381 aggtaaaaac caagcttata gattaaaaaa taaatgattt atagatgaag aactaaactc
     4441 atagattaaa aaataaatga cttatagatg aaaaatcaag ctcatagatt aaagaataaa
     4501 taacttatta gatgaaaaaa caagcttata gataagaagt ccatgcttat aagccagaaa
     4561 atgaatactt taatagatta aaaaatacat atttgtgaat tatagaatga atgactccat
     4621 aaactagaaa atttataata atatctatat gaaaattaga attattttat cacaaaaaaa
     4681 ttaagttaaa taaatatgaa attacttcca aatatacttc caaaaacatg taatcgcata
     4741 tataatataa ttataaaaat aataaatatt tttatagtag tagttaatta ttaataagga
     4801 ggggtcaaaa tgcctagcat gattatggaa gttgtaggag tactatttat agctattgga
     4861 ttaatcttga cattcattga aataaaagga aatttccctt ctttttatgg ttttggtcaa
     4921 aattctatag ggaaaaatgc tgtaggtatt atagcagcag gaattgcttt aattttcttt
     4981 agaaatggca tagatagcat agattctata gctacttatg gtgtattttt agcaatagaa
     5041 cttgttgtaa tagttgcata tttcttaata agaaaaaaat ttactaaaga agataaacat
     5101 ttaagaacta attaactaaa ttatctacta taaaattgga aaattaaaaa caaataaaag
     5161 tttgttttta attatttaaa atccaaattt gattattttt aaagaaacta tgttctgtat
     5221 aaaactagaa tgtggtttct tttttatgta taaaatattt tttagcaaaa gttaaaaggt
     5281 gttttataat ataaaatagt taattgtaga tgttaataaa agaaaattat gtatatttca
     5341 gataaagata agagagtata ctatatttta attctttttt caataaagct tattgaaaag
     5401 aactttaact tagaagagta tcaccaaatt gcagatacaa tatcataagc agttatttat
     5461 tgacaaccct ttttaataaa tatatcattt gaagtatact gacgaaaagg agttgaataa
     5521 taatggacat cttattggtt gaagatgata agaatattcg agaaatggta tacgattttc
     5581 tgattagtga gaattactct gttaaaactg ccattgatgg aaatacggct attgaaatat
     5641 ttaaaaatta ttcatttgat attattttac tggacttgat gttaccaaag tctagtggtt
     5701 ttgaagtatt aaaatatata cgaagtgtga gtgttgttcc tgttattatt actactgcga
     5761 aagatagtga cacagataaa atgctagggc ttaatcttgg tgcagatgat tatattacaa
     5821 agcctttttc aatcgtagaa ttgcttgcaa gaattaaagc taatattaga cgtgcaacag
     5881 tgtacagcag ttcaagaaac tctcaagata tggttattca gtataaagat ttgattataa
     5941 acatatcaaa atatgtagtt tcaaagaatg gaattgactt aaacttaaca catacagaat
     6001 ttgaaattgt taaattacta gcgactaatc aaggatgtgc tttttcaaag gaacaattat
     6061 acaacgctat ttggaaagaa ccttattatg gaaatgaaaa tgtgctaaat actcatatta
     6121 acaggttaag aaataaattg tctgatggta tagaaaaaaa taattatatt aaaactcttt
     6181 ggggaattgg ctacaaaatg gaggaagaat aatggagtta tttttagttg taatttcgat
     6241 tgtactattt gctctctact taaaagaacg aaataaatca aagaaaatgg aaagtgatta
     6301 taagtgtatc aattcaagac ttcgtgacat aacaaatgat gatgaaatta attatatttt
     6361 agtaccatct gatatttcga ttgtaaaaga aacagcagta gatataaata aacttcttga
     6421 aaagttttac agcaggcaaa ttaattataa tagaagtgaa aaagcgatat tgcaaatatt
     6481 tacaaacata tcacacgatt tacgaactcc catcacagta ttaaagggtt atattgaaat
     6541 gctatatctt caaagtcaaa aagaagaatt atcttcttca atgagagcaa ctgtggaaaa
     6601 aatgcaaaca aattcaaatg agcttgttaa ttcaataaat aacctgttta atatggcaaa
     6661 aatccaatct ggagatatga tattaaatct tcaaaaatcc aatctaacac aattatgtca
     6721 tgagattatt ttagagtttt atgatttgct agataaagaa aactttaatg ttgaagttaa
     6781 tattgaagat agacctatat atgcaaatgt tgacacagaa gcattacaac gtatactcaa
     6841 aaatttaatt gataatgcta taaaatatgg ttcagatggt agatttttag ggatttcact
     6901 atatgaaaag gaaaaacatg tttttataga tattgaggat aaaggttcag gtatttctga
     6961 aactgaaaaa gagcatattt tcacaagagc ttatactgct gacagaaaaa aaggcaatgg
     7021 tttaggtttg gctatttctc aagggcttgc aaactctatg ggaggttcaa tttatgtaaa
     7081 tagtagacct aatgtaaaaa cagtttttac ggtaaaattg aaaagttaga aaaaagttag
     7141 atttaaaaga gagaaaagac aattttattt tgtatactgt ttttagaaag gagggagtat
     7201 gatgaactat atcatagaga cccatcaatt aaagaaaata tataaagata aagctgttgt
     7261 taatgctgtt aatattcatg tgaaaaaggg agaaatttat ggatttgttg gaccaaacgg
     7321 ggcaggtaaa agtacagtaa tgaaaatgtt gctaaatctt gttaagccaa attctggaga
     7381 aatagttatg ttcagtaaga aagtggaaga aactgatttt gaaatattaa aaaagattgg
     7441 tactattatt gaaaatccgt atttatatga gaatttgact gctaaacaga atcttgattt
     7501 acactgtgaa tatatgggtt attataacaa agaacatata tcagaggtat tggaatgtgt
     7561 cggattgtca aagcaatcaa acaagaaagt ttcaaaatat tctttaggga tgaaacaacg
     7621 tcttgcaata gctagagcaa ttcttactaa accagaatta ttgattcttg atgaacctat
     7681 aaatgcactc gacccagagg ggataagaga aatgcgagaa ttattccgta agttaaatac
     7741 agattatgga ataactatat ttatttctag tcatattctt tcagaggtag aacaaattgc
     7801 ggacactatt ggaattattc aagatggtaa acttattaaa gaaatttcta tgtcagatat
     7861 acacaaatat caaacagatt atgttgaagt agatgtagat aatgtggagc ttgctggata
     7921 cttgttagaa aaagaatttg gaataaaaga ctttaaaatt acttcggaaa gttgtattga
     7981 aatatatgat ttacgaaaag atgtaaaaga aatatccaaa atatttattc aaaatcaaat
     8041 tggtataaat agcattggac gtaaacaaag ttctttagaa gattacttct tccaaactac
     8101 aggtacagga ggtaaagaaa cactatgata catttaatga aactagagct aaaaaaagtt
     8161 gggctaaaaa gatatttttt attttcaatg ctggggattt tcttatgtat gttttttgtc
     8221 tttactggat tgaatgataa atctacttca atatatgatt atgatgttac atttaggaca
     8281 atcagtttat ttttctgttt ttactatatt atattatttg ctgtgttgaa tgtggcatat
     8341 ataataaatg aatatacaaa taaaacaatc ttgattttat tctcttatcc acttgaccgt
     8401 aaaaaactta ttttatcaaa gttacttttg attacgactc ttatgatatc atcaatgata
     8461 gttggatata tctgttgttt tacattcgtt gttgtgttgg ataagtattt taatttagtt
     8521 attggagatt ttcatatttc aattttatct tattggatac caggagcaat taaaagtatt
     8581 attatatttt gctcattagg tatatggaca ttcgttgttg gaatgataag aaaatcaata
     8641 cctgttaccc ttgtcagttc aattattttt atcaatttaa gacaatttat tttagctggt
     8701 acaaatacaa ctcaagacag tttgatttct gtttttatta tagttggtat aactgtgatt
     8761 ggtatatatt atatactaaa tcataaagtt actgaaattg actagatact gtggctagga
     8821 aggtagttat taattgtatc ttcttgggat aataatgatt tgctattata tcagctatta
     8881 ttaatattat tagagtttta ataatattgc acaaccattt gaagttgagc caaagcttga
     8941 tgaattaatt gaatggtact atagtcagag tgaagtatct attaaagtta tagctgaatt
     9001 tcattataga tttgaattga tacatccatt tcaagatggt aatggtcaca taggtagatt
     9061 tgttatgcta aaacagatgt tagaaaataa tcttcctata aaaattgtat cttgggatag
     9121 tgaggacttg tatagaaact ccttaaattc ttgtagttta ggtaattatg tccctttaat
     9181 tgaatattta tcaagtttag aagattttag agaagtatat aaaatgttat ggaaataatt
     9241 gtttgcagca aaaaaaaatt ttatataaat atatttttat ctatgctatt taacgctaat
     9301 ttcttagtta ttcttagctt ggactatatc cactatttta actatataaa atagtggata
     9361 tagtttaact atctctttat aactattctt atgtatctta gttgtttctt aatatgggga
     9421 cagttcataa aactaaaagt gatttttaat tttatcattt ataatttacc attacatcag
     9481 aaaaatgctt atcattagaa ttttcataaa agtcacctat ttttttcaat taactaaatc
     9541 aattctatta gtcttaatct ttcgtatttc aaattgataa tataagaaaa taaaaaataa
     9601 aaaattttaa attttttatt tattgcgaaa aaaataaaaa ggtgctatac tttttgtaac
     9661 atattattca aattattaag agatagtaaa catttatagt ttaatttgta gtacactagc
     9721 atgatttgca gaacaatgaa ttaatctata gtataatagt ttaatttaaa gtacaacgat
     9781 ttaatctata gtacactagc atgatttgaa gtacaacgat ttaatctata gtacaataac
     9841 ttgatttgca atacaatgaa ttaatctatg gtataatagt ttaatttgaa gtacaatgaa
     9901 ttaatctata gtataatagt ttaatttgaa gtacaacgat ttaatctata gtataatagt
     9961 ttaatttgaa gtacaatggt ttaatctata gtataatagt ttaatttgaa gtacaacgat
    10021 ttaatctata gtacaataac ttgatttgca atacaatgaa ttaatctata gtataagagc
    10081 atgatttaca gtacaataat ttaaattgta gtacaaaagt actatcaaat aagaatttct
    10141 actttaaaaa tattactaaa agtaattaaa gtccagtaat tcaatataat atggcaaaag
    10201 aatagaaata aggtgagagt agtgaaagta aagtcaaatt tagatgaaat tttatatcat
    10261 aagattattg aaagtcttat tcgtggtgaa tactctgtag gtcaaaaaat attactaaat
    10321 gacctttgtg aaaaatttga agtaagcaga acaccagttg tacaagcagt aaaaatgcta
    10381 aataaagatg gagtgcttac tattatgaca aatggtaaag tgtacgtacc agagtatgaa
    10441 tatgatatgg taaaacaagt ttgtgaaacg agaacactga ttgaaacata tgctttagaa
    10501 aaaatgatgc aagaagagga agaggttttt caacaaaagc tagatgtaat aaaaagatac
    10561 tctgataaat gcgaggagta ctatcgtcaa gaaaaatctg tagaacttgc actagcagat
    10621 ttggaattac ataaagccat tgtagaaggg gcaaataatg agattttaaa tgatgtatat
    10681 gttggtatac aaggaagatt tatagtagtg aactatttaa ttcgaccatt aaaaaatcgt
    10741 aattatgaag gaacagtaca agaccatttt gaaatattaa aattcattga agacagagat
    10801 acaaaaaaag cagtagataa actaagaaat catattcaag gaactattaa aagattctgt
    10861 gaagatgaat aataagtttt gaagttacta tatatactta tctattaagt tgctaaatgt
    10921 agtctaagta taatctaatt atagtttaaa cataatctaa atataatcta ataaatataa
    10981 tctaaatata gtgtaaataa gtctaagtat gaattaaata tagattaagt atggactaag
    11041 tatagattaa gtatagatta agtatagatt aagtatagat taagtatgaa ttaagcatgg
    11101 attaaatata ctttaaatta caaatattac aaggaagcca gaaggcttcc taaaaaaata
    11161 acctttataa aattttttat tttaaattta aaatttttta ttttaaattt aaaataaaaa
    11221 atgaaagagg tggtagggag tttgaaaaaa attactaaag aaaggacaga aaatactatg
    11281 aaaaagctat ttacgagtga aacatttttg gaatatcaat ttataagtga ttgccaaata
    11341 tcaccagatg gaggatatac agcatttatt gtaaaaaaag ctgatatcaa agagaatggt
    11401 tacacaagcc aagtttatat attaaacaat aaaactggcg aattaaaaca aataacgtct
    11461 ataaactctg ttggagcata tgcctgggaa gatgaaaata ctatcttgtt ccctgctttg
    11521 agaaatgaaa aagtaaaaga agctgtcaaa aacggtaagc aatgcatgag ttactatgct
    11581 ttatctttaa atggtggaga agcagaagaa ctttttagac tccctatcaa aggtggtaaa
    11641 ttgaatccta taggaaaagg tttgtatgca gttattgatt cttatgacaa tgaccgacct
    11701 attgtagagg gattgccaga agaagaacaa cagaaaaaaa taaatgctta taacaaaaga
    11761 cattatgaac atttcaaaga aattccttat gctgtcaatg gggagggata tattagcaga
    11821 aaaagaaaac gcctatatat atacgacagt aatataaatg agttgaaggc aattacagca
    11881 cctatgttta atgttgtagg tatgaaaata agtgatggaa aaatcttata tatagggcaa
    11941 gagtttaaag atgtaaaagg tttgaaaaat ggggtctatg tatttgatac aaacacaaac
    12001 acaaatgtct gtatattaga aaaagataag tatattataa aaggttttga attatatcaa
    12061 aatcaagtga tactaaacct aactgatgct ttatcttatg gaaatggaga aaatggagat
    12121 ttttatacta ttgatattga tacgaaagaa atgaaacttc tttcagggca tcaacatcat
    12181 tgtattggaa atacagtaac atctgatgtt aaaatgggtg ctggtcagac aacaaaggta
    12241 gatggagaat atatttatta cacttctaca gtagatatgg actgcattat tgaaagaata
    12301 catattccaa caggaaaaca ggaaaaagta acacagacag gttcagttga ttttatagat
    12361 gtaaaagatg gcaatattgt atgtgtgggt tgtttaggaa atggattacc agaagtttat
    12421 acagtagaga atggaacatt gtgtaagaaa acacatttaa atgaccatat tttagaggaa
    12481 tataaaattt ctgtgccaga atacatagag tcaaaaggaa gttctaaatg ggaaatacaa
    12541 ggatacgtat ataaaccagt agattatgag gtggggaaaa aatatccagc tgtattggcg
    12601 attcatggag gaccaagatt gacatatgga ccatatttta tgcatgagat acaggtattt
    12661 acaagtgcag gttactttgt attcttctgt aacccaaggg ggtctgaagg tcgtggcaat
    12721 gcttttgcag atattaggaa acagtttgga gatattgatt atatagattt tatggaattt
    12781 acagatactg tacttgaaaa atatacagac attgataaaa caaaattggc tgttgaaggt
    12841 ggaagttatg gtggatttat gacgaattgg ataattggtc atacaaaccg ttttgctgtt
    12901 gcttgtgctc agcgttctat tgcaaactgg agtggcatgg agggtacaac agacattggt
    12961 tattatttct gcaaaggaca aacaggtgct tctcatatgg aaaaccacga gcttcaatgg
    13021 aaacagtcac ctcttgctta tgctgataaa tgtgttacac cgactttatt tttacatggt
    13081 gaaaaagact atagatgcta tatgcaagaa gcattccaga tgttttcagc attgaaaatt
    13141 catgggtgtc ctacaaaact ttgtctattt gaaggagaaa accatgaact gtctcgttct
    13201 ggtagaccaa aacaaaagct acaaagatta gttgaaatgc tagattggtt ttcggtatat
    13261 ataaaaaaag ataacatagg acagtagaaa ggagaatata aaaatggaaa aaataaagat
    13321 tgaaagttca gatatttttg aatatatatt ccctcatgat atttcttgtt cacctgatgg
    13381 aaagcatatt gcttatatta tatcaaatat caacgaggaa aaagactgct atgaacatga
    13441 cttatatgtg atggacatca aaacagaaaa acaaatacat atgactcaaa caaaagatgt
    13501 aacatcattt agttggattt ctaatacaga actccttttt acatctaaaa gaaataagcc
    13561 aaaatcaggt acaacagatt tttacactat ttctatagaa ggaggagaag caaaaaaagc
    13621 attttctatt ccaaaagctt gtagcgtacc tgtatcactt ggaaataaat tgtggttact
    13681 gacaacaaaa aatccaacgg attctaaaaa atcagaacca gatagggcag tagagggtgt
    13741 agattattgg acatttacag ataaaccttt tataagagat ggagaaaatt tttctcaaag
    13801 aagacgtgta acattagaat tatatcaaga aggtgaaaat ataacaaaag ccattacacc
    13861 aaaattttgt gaagtagcag gtgtagacgt ttcttctgat aaaaacagaa ttttatatac
    13921 tggtcagatt tatgaagatt gtgcaactcc tttttctgga ctttgggagt atcatataga
    13981 cagtggtgaa accaaagaac ttgtaccaca aggaaagtac caaataagtt tggcaaaata
    14041 tattggcaaa gataaagtaa tgttgcaagc ttcaacatta gaccgttcta tcacacagaa
    14101 tcatgatatt tttatacttg atttgagtac taatgaaata aatatgattg ctagtccaga
    14161 tggtatgtat gctacattgc tggatgtaga tgcagtatat ggtggtggac gtagtaataa
    14221 agttatagga gataaattta ttggtgcaag aatttgcaga actatgacag aatttaatga
    14281 gtttgataca aagacaggta atatacgtat catcacaaaa gtagatgctt ttacatcttt
    14341 tgatatttac gacaacacca tgtacacagt aatgttgaaa gattatgagt tagcagaaat
    14401 atatagcatt gatatgacta caggtacaat gaaaaaaatg actgcttttt ctaaaccata
    14461 tttagatact cataaggttt cattacctga aaaattgacg tttgtagcaa aaaataaaga
    14521 agaagttgat ggttttgtta taccacctat agatgctaaa gaaggcgaaa aatatcctgc
    14581 tgtattgttc attcatggtg ggcctaaatg ggcatatggc tatatgttta cacacttgaa
    14641 gcagtgtgta acatcaaagg gaatgtatgt gatttattgt aaccctcatg gtggtgacgg
    14701 ttatggtgaa aagttcttag aaatggtaga aagatggggg tatgttgact atgagcatct
    14761 tatggaattt gtagatactt gtattgaaaa atatcctggg attgatgcag accgcttagg
    14821 ggttgcaggg ggaagttacg gtggttatat gacaaactgg ataattggtc atacagaccg
    14881 tttcaaagca gcagcttctc aaagaggtat tagtaacttg attacagctt ctttaattat
    14941 tgactttgga gatagaatta tgaagcagac ttgtggagat aagacgcctt ggaatcatga
    15001 ggaggttctt tggaatcatt cacctattaa atatgtgaaa aatgttaaga caccaacatt
    15061 gttcttacat agcgataggg attatagatg ttttatgggg gatactttcc aaatgtttac
    15121 agcattaaaa cagcttggtg tagatacaga aatgtatttg tttcacggag atacacatgg
    15181 attgtctaga aatggaagac catctaatag aattgcaaga gcaaatgcta ttgtagattg
    15241 gtttgaaaga tatttataat aagggggtta ttctatgagt gatatgttat tatggtttag
    15301 tgtcgtatta gttttatttg ctattgggga tttaattgca agcaaaacaa aagcaaaagt
    15361 atcagctgta tttgttacac tacttttatt tttgattcta tttgtcacaa aagcaatacc
    15421 agctgatatt attgaaaaag caggtatgac cgcagcagct agttggagtg ttccaatgat
    15481 tatgtttagt atgggaacaa tgttgaatgt taaacaattt atagacgaat ggagaacagt
    15541 attgacagca tggcttggaa ttgtagcagt tatagtctgt gtctcattat gtattccttt
    15601 gtttggaaaa tcaacagtat tgacaagcat accagttata aatggagctt tacctgctac
    15661 tactattatg acacaagcag cactagaaaa aggactgaca cttgcagcag ctacagcaac
    15721 agttgtattt gcaattcaga agtttattgg tacaccaata gcttcaagag cagcgttaca
    15781 agaggcaaac cgtcttttag tagaatatca tgaagcaaaa tcaaaaggca ttgatttagc
    15841 aaatgttgat actgataaga aagaagcaga gaatacaggc acaaaagtaa aacaagcttt
    15901 ctgtgaaaaa tacgataaat attattctac aaatgtttgt atatttttta tagcactatt
    15961 ctcttatcta gggtatgaac tttcagagat tattcatgta aattattcta ttgtatgtct
    16021 tgttgttggt gttattgtaa cacgtattgg tattgtacca aaagacattt tggaaaaagg
    16081 aaaaatcaag ggatttatca acatggttgt gtttgcagct gttattcctt ctttagcaaa
    16141 agtttctttg acagatttaa ttagtctttt tgtaccaatt gtgggtatgt ttgcagcttc
    16201 tatcattggt atattcctta tgatgaaggt gttaccaggt tggaaaatta tcggttctaa
    16261 acctcttgct tttggagtag ggttttgtca gatgttggga ttcccaacta catatttaat
    16321 ttcaaatgaa gtgtgtaatg ctgttggaga aacggaagaa gaaagagcat atttgatgtc
    16381 taagattatg cctaagctgg tagttggtgg tatggcttgt atgatttcta ttgtggtagc
    16441 tggtcttatg gtaccaatgt tgtagatagg tttcatagtt aaaaaagtgg tgttaattta
    16501 taggagaagc taaaaagtag tattaattta taaaataatt ttatattagt taatttgtta
    16561 taagaatatt ttctttttat aattataatt ttgattttat aagagagcag attttttatt
    16621 tgctatgcta atagttataa tttgttttta tttgctaaaa tgca
//
LOCUS       DAELNY010000075        16574 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.075, whole genome shotgun sequence.
ACCESSION   DAELNY010000075 DAELNY010000000
VERSION     DAELNY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.075"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..593
                     /gene="tuf"
                     /locus_tag="KPE55_002068"
     CDS             <1..593
                     /gene="tuf"
                     /locus_tag="KPE55_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG0493132.1"
                     /translation="IPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEV
                     ELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVK
                     AHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVT
                     MEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            843..992
                     /gene="rpmG"
                     /locus_tag="KPE55_002069"
     CDS             843..992
                     /gene="rpmG"
                     /locus_tag="KPE55_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG0493133.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            1018..1239
                     /gene="secE"
                     /locus_tag="KPE55_002070"
     CDS             1018..1239
                     /gene="secE"
                     /locus_tag="KPE55_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG0493134.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1259..1801
                     /gene="nusG"
                     /locus_tag="KPE55_002071"
     CDS             1259..1801
                     /gene="nusG"
                     /locus_tag="KPE55_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG0493135.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1840..2265
                     /gene="rplK"
                     /locus_tag="KPE55_002072"
     CDS             1840..2265
                     /gene="rplK"
                     /locus_tag="KPE55_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG0493136.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2336..3034
                     /gene="rplA"
                     /locus_tag="KPE55_002073"
     CDS             2336..3034
                     /gene="rplA"
                     /locus_tag="KPE55_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG0493137.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    3086..3214
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3257..3763
                     /locus_tag="KPE55_002074"
     CDS             3257..3763
                     /locus_tag="KPE55_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG0493138.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3821..4186
                     /gene="rplL"
                     /locus_tag="KPE55_002075"
     CDS             3821..4186
                     /gene="rplL"
                     /locus_tag="KPE55_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG0493139.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4578..5366
                     /locus_tag="KPE55_002076"
     CDS             4578..5366
                     /locus_tag="KPE55_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0493140.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5837..9553
                     /gene="rpoB"
                     /locus_tag="KPE55_002077"
     CDS             5837..9553
                     /gene="rpoB"
                     /locus_tag="KPE55_002077"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG0493141.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9595..13080
                     /gene="rpoC"
                     /locus_tag="KPE55_002078"
     CDS             9595..13080
                     /gene="rpoC"
                     /locus_tag="KPE55_002078"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG0493142.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13369..13791
                     /gene="rpsL"
                     /locus_tag="KPE55_002079"
     CDS             13369..13791
                     /gene="rpsL"
                     /locus_tag="KPE55_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG0493143.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13915..14385
                     /gene="rpsG"
                     /locus_tag="KPE55_002080"
     CDS             13915..14385
                     /gene="rpsG"
                     /locus_tag="KPE55_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG0493144.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14434..16500
                     /gene="fusA"
                     /locus_tag="KPE55_002081"
     CDS             14434..16500
                     /gene="fusA"
                     /locus_tag="KPE55_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0493145.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6506 a         2066 c         3395 g         4607 t
ORIGIN      
        1 atataccagc tccagagaga gatacagata aaccattctt aatgccagta gaggacgtat
       61 tctcaatcac aggaagagga acagttgcaa caggaagagt ggaaagagga gtactaaaag
      121 tacaagacga agtagaatta gtaggattaa cagaagcacc aagaaaagta gtagtaacag
      181 gagtagagat gttcagaaaa ttattagacc aagcacaagc aggtgataat ataggagcat
      241 tattaagagg agtacaaaga aacgagatag aaagaggaca agtactagca aagactggat
      301 cagtaaaggc acacacaaag tttacagcag aagtatatgt acttaaaaaa gaagagggtg
      361 gaagacatac accattcttt gatggatata gaccacaatt ctatttcaga acaacagacg
      421 taacaggagc ttgtaagtta ccagaaggaa tagagatggt aatgcctgga gataacgtaa
      481 caatggaagt agacttaata aactcaatag ttgtagaaga gggattaaga ttctcaataa
      541 gagaaggtgg aagaacagta gcttcaggag ttgttgctac aataatagag taattcttta
      601 ctaaactgat ggattaaaag gaataacaaa aagaggggtt atgctcctct ttttttaaca
      661 tagaaataca aaaacattga cacagcatag gattttgtga taacttatac aaggtaatga
      721 ttataggaag ttaataatat cttgaatact gtagattttt aataaataca tattttaaag
      781 atattatatt cacatatatt aacaatatat attattgtat tacaaagttg gaggtgtatc
      841 agatgagagt taaagtaact ttagcatgta cagagtgtaa gcaaagaaac tacaacacta
      901 ctaagaataa gaaaaacaat ccagacagaa tagaattaca aaaatattgt agattctgta
      961 aaaagcatac aactcataaa gaaacaaaat agttttttat aaggatgtga gtatggaatg
     1021 gctgcccaaa aaaatgacgg tgcaaaaact aaaaaaaggt ttagtttgtt tggatactta
     1081 aaagaaacta aacaagaatt aaaaagagtt acatggccga caaaaaagga actgtttaaa
     1141 aacacaagta tagttttaac tgtagttatt tcatgcacta tattagtatg gggtatagac
     1201 actatattat ctggtgcact agcgttatta ctaaaataga cgaaaagagg taagttaaat
     1261 gtcagaatta caagaagcaa gttggtatgt agttcatacc tattcgggac atgaaaataa
     1321 agttaaagct acaattgaga aagcagttaa aacaagaggt atggaagact gcataagaca
     1381 agtagttgtt ccaacagaag aagtagtgga aactactaaa actggaaaag aaaaaactag
     1441 acaacgtaaa gtttatccaa gttatgtttt ggttaaaatg ataataactg atgaatcttg
     1501 gtatgtagta agaaatacta agggagttac aggtttcgtt ggacctggtt caaaaccagt
     1561 gccattaagt gaagacgaag tcaaagctat ggggatagac acaacagacc ctaaagtagt
     1621 aaatagcgat attgattttg aaataggaga tacagtaaaa gtatctcaag gaccatttag
     1681 tggacaaata ggtaacatag aagaaattga cttagaaaat agagaagtca aagtatgcat
     1741 aaatgcattt ggtaaaagga ctctatttgt aatagagctt gaaggtatag aaaaaatcta
     1801 atactatatt atagaaaaat ctgaggaggt gcgctcaaaa tggctaaaaa agttataggt
     1861 caaataaaat tacaaatacc tgcaggaaag gctactccag cgccaccagt tggaccagca
     1921 ttaggacaac atggtgttaa tataatggga ttcacaaaag aatttaatgc taaaactgca
     1981 gatcaagctg gaatgataat accagttgtt ataactgtat atcaagatag atcattcagt
     2041 tttataacaa aaactccacc agctgcagtt ttaattaaaa aagcattaaa cttaaaatca
     2101 ggttctggag aaccaaacaa gaaaaaagtt gctaaaatga cttcagctca agtaagagaa
     2161 atagctgaat taaagatgcc tgacttaaat gctgcatcag tagaagctgc tatgagtatg
     2221 atagctggta ctgctagaag catgggtgtt gtaatcgaag actaattaaa agatacactt
     2281 ttttttataa agtgggaggc tagaagccga aattaaccac gaggaggaaa ataaaatggc
     2341 taaaaaaggt aaaagatatg caggtgcatt acaaaaagta gatagaacta aattttatga
     2401 tgcatcagaa gcattaacat tagtttcaga tatagctgga gctaaatttg atgagacagt
     2461 agaagcacac attaaattag gtgttgactc aagacatgct gaccaacaag taagaggtgc
     2521 agttgtatta cctcatggaa ctggtaaaac aaaaagagtt ttagtttttg ctaaaggtga
     2581 aaaagctaaa gaagctgaac aagctggtgc tgactttgta ggagctgaag aattagttca
     2641 aaaaatacaa ggtgaaaact ggtttgactt tgatatagta gttgctactc cagatatgat
     2701 gggtgtagta ggtagattag gtagagtatt aggacctaaa ggtttaatgc caaaccctaa
     2761 atcaggaaca gttacatttg atgtagctaa agctatagat gaaataaaag ctggtaaagt
     2821 tgaatacaga ttagacaaaa ctaacataat acacgttcca gtaggaaaag tatcatttgg
     2881 tggagaaaaa ttaactgaaa actttactgc attaatggat gctataataa aagctaaacc
     2941 agctgctgct aaaggacaat atttaagaag tataacagta gcttcttcaa tgggacctgg
     3001 agttaaaata aacccagcaa gaacagctga ataataaaac tgttgacaca ggttgacaaa
     3061 aatgttatta tatcacttgt caatgtaaaa aagaatagat ttgccgtaga tagtgggtgc
     3121 gagagcttaa tatcccgccg aggttttaga taaattgaga ttactaaagc ttttgatgtc
     3181 tacttttggc aacaaaagct ttttataatg ttgcggtaaa tactaagcct atttggtgaa
     3241 ggaggtgcac atctatatga gaaaagctat agaaattaaa tcagaagttg tttctgagat
     3301 agttgaaaag ttacaaaaat cttctgctgc tgttgttgta gattacaaag gattaacagt
     3361 tgaagaagta actgagttaa gaaaacaaat gagagaagct ggtgtagatt acaaagtata
     3421 caaaaatact ttagttagaa gagcagctaa agaagttggt atagagcaat ttaatgatga
     3481 attattagtt ggaactaatg ccatagcatt tggatatgat gatccagttg ctccagctag
     3541 aatattaaaa ggattcatgg attctcatcc aaaaatgaaa ttaaagatgg gtattgtgga
     3601 aggtgcattc tatgatgaat ctaagattgt tgaaatggct aacataccat caagagaagt
     3661 tcttatcgct aaattacttg gtagcttaaa agctccagta tcaaacttcg catacttaat
     3721 agatgcaata gcgaaaaagg cagaaggtca agaagaagct taattgctta actaaaaaat
     3781 tataaatatt aatttgaaat aaaattcgga ggtgctaaaa atgacaatag aacaaatatt
     3841 agaagctata gaaaatatga aagttttaga attaaatgaa ttagttaaag ctgctgaaga
     3901 aaaattcggt gtatcagctt cagctccagt aatggtagct ggtgcagctg ctggtggacc
     3961 agctgctgaa gaaaaaactg agtttgatgt agtattaact gacgttggtt cttcaaaagt
     4021 tggagttata aaagcagtta gagaaataac aggattagga ttaaaagaag ctaaagaagt
     4081 agttgacaat gctcctaaga cagttaaaga aggagcttct aaagaagaag ctgatcaaat
     4141 aaaagagaaa ttagaagctg ctggagctaa agtagaagtt aagtagtttc tttaaaaaga
     4201 tacccattgg gtatcttttt ttatttataa aaaactataa agaaagtatt tggaattata
     4261 aagaaagtat ttttataaaa taaaaaaaat ttatataata gtactaaatt aagggtatca
     4321 tatatggtgt aatggtattt taatttaaca ttattatcaa attaaaatat aaacaattgt
     4381 tgtaatgtct atatataaag tatataatta aactaaaatg taaaatttta cattttagtt
     4441 taaaaaaata cacgagtgtt ttgtaaataa aatgtaataa ataattaatt aacaaatata
     4501 aattttaaaa taagttagat atttagaaaa atcgaaattt gtaggattta aaggacatat
     4561 tcaaggagga gttaataatg gaaaaattac aaggaaaaat tgcagtagtt actgcagcaa
     4621 caaaaggtat tggattagca tcagcagaga tattagcaaa aaatggagca actgtgtact
     4681 tagcagctcg ttcagaagaa ttagctaatg aagttataaa taaaataagt gcagaaggtg
     4741 gttgtgctaa gtttgtttac tttaatgctc gtgaagaaga gacctttacc tcaatgatag
     4801 aagaggtagt taaaaaagaa ggtaagatag atatacttgt aaacaatttt ggttcaacaa
     4861 acccttctct tgacaaagac cttgtaactg gggatacaga taatttcttt gacacagtaa
     4921 atactaattt aaaaagtgtg tatttaccat gtaaagcagc aattcctcat atgataaaga
     4981 atggaaaagg aagtatagta aatatatcaa gtataggttc agtattacct gatttatcta
     5041 gaatagctta ctgtgtatca aaagcagcaa tcaactcatt aactcaaaac atagctacac
     5101 aatatgctaa agacaatgtt agatgtaatg ctgtacttcc agggcttatt gcaactaaag
     5161 cagcattaga caatatgtct ccagagttca taaaagaatt tttaaagcat gttcctttaa
     5221 atcgtatagg ggaaccagat gatatagcaa aagctgttct attctatgct agtgatgact
     5281 catcatttat aacaggggat ttacttgaag ttgcaggagg atttggttta cctactccac
     5341 aatttgcaga taatatatta ggataattaa attactaata tgcaatgaaa gcttatgcat
     5401 tagttgtagt agaaatgata tttttataaa gaccatagat ataatttata ttggaaaggt
     5461 ttgaggttca aaattccaag ttaaatttat ttatggtctt tattttgtat attttaataa
     5521 attgtatata tttgtataaa tgtaattatt tacaatcatt ttaatatgga attataatta
     5581 aatagggggg atggtacttg tataaaattt tttatagaaa attagaccta taaatgaaaa
     5641 atatggattt tatattggaa aattgtacat aatacataat atatgcttaa aataatttac
     5701 aaaaaatcaa aaataatgca tagtttacat ataagataaa aaatatattg aaaaatactt
     5761 gacattatgc taaaattatt agatgcgtta gaatgaacta ttgttttatt tttaattatt
     5821 gagaggtgaa aagtgaatgc cacatcctgt cacgataggt aaaagaacta gaatgagctt
     5881 ttctaaaatt aaggaaatag ctgatgtgcc aaatcttata gaaattcaag tagattccta
     5941 tgagtggttt ttaaaagaag gtttaaaaga agtgtttgat gatatttcac caatagaaga
     6001 ttatactggt aatcttatat tggaatttgt cgactattct ctagatgata aaccaaagta
     6061 cgatatagaa gagtgtaaag agagagatgc tacatattgt gcacctctaa aggttaaggt
     6121 aagacttata aataaagaaa caggtgagat aaaagaacaa gaagtgttta tgggtgactt
     6181 ccctttaatg acagaaagag gaactttcgt tataaatgga gcagaaagag ttatagtaag
     6241 tcaattagtt agatctcctg gtgtttacta tgcggaagaa agagataaaa caggtaagag
     6301 attgatttca tctactgtaa ttcctaatag aggagcatgg ttagaatatg aaacagattc
     6361 taatgatgta atatctgtaa gagttgatag aacaagaaaa caaccagtta cagttttact
     6421 tagagcttta ggaataggaa cagatgcaga gataattgac cttttaggag aagatgagag
     6481 attatctgct acattagaaa aagataatac taagacagta gaagaaggtc ttgtagagat
     6541 atacaaaaaa ttaagaccag gtgaacctcc tactgtagaa agtgcatcat ctttattaaa
     6601 tgctttattc tttgacccaa aaagatatga cctagcaaaa gttggtagat ataaattcaa
     6661 taagaaactt gctttatgtt atagaataat gaataaaatt tcagcagaag acataataaa
     6721 tccagaaact ggagaagtat ttgtaaaagc tggagaaaaa ataagctatg atttggctaa
     6781 agccatacaa aatgcaggta taaatgtagt aaatctacta atggatgatg ataaaaaagt
     6841 tagagttata ggaaataatt ttgtggatat taagtctcac attgattttg acatagatga
     6901 tttaaacata aaagaaaaag tacattatcc aactttaaaa gaaattttag atggatatag
     6961 tgatgaagaa gaaataaaag aggctataaa atcaagaatt aaagaactta taccaaaaca
     7021 tatattatta gatgatataa tagcttcaat aagttatgag ttcaatatat tctataatat
     7081 aggaaatatt gatgatatag atcacctagg aaacagaaga ataagatctg taggtgaatt
     7141 gttacaaaat caagttagaa ttggtctttc aagaatggaa agagttataa aagaaagaat
     7201 gacggttcaa gatatggaag ctataacgcc tcaagcgtta gttaacataa gaccagtttc
     7261 agctgcaata aaagaatttt ttggtagttc tcagttatct cagttcatgg atcaaacaaa
     7321 tcctctatct gagttaacgc ataagagaag attatcagcc cttgggcctg gaggtctttc
     7381 aagagaaaga gctggattcg aagtgcgtga cgttcaccat tcacactatg gtagaatgtg
     7441 tccaatagag actccagagg gaccaaatat cggtcttata aactctctag gaacttatgc
     7501 aaaaataaat gaatttggat ttatagaatc accatataga aaatttgata aagaaacatc
     7561 aacagttact gatgaaatac attatttaac tgctgacgaa gaagatttat tcgtaagagc
     7621 tcaggcaaat gagccattaa cagaagatgg taaattcgta aaccatagag ttgtttgtag
     7681 aactgtaaat ggtgctgttg aaatggttcc tgaaagtaga gtagattaca tggatatatc
     7741 tcctaagcag gttgtatctg ttgccactgc tatgatacct ttcttagaaa atgatgatgc
     7801 caaccgtgcc cttatgggag cgaacatgca gcgtcaggca gtgcctctag ttagaagaga
     7861 agcaccaatt ataggaactg gtatagaata cagagcagca aaagactctg gagcagttgt
     7921 tgttgctaga aattctggta tagcagaaag agtaacagca gatgaaataa ttataaaaag
     7981 agaagatgga aacagagata gatataatct acttaagttt aaacgttcaa actcaggtac
     8041 ttgtataaat caaacaccta taataaataa gggtgaccag ataataaaag gtgatgttat
     8101 agcagatggt ccagcaactg atttaggaga agttgcactt ggaagaaact gtcttatagc
     8161 attcatgact tgggaaggtt ataactacga ggatgctata ttaataaatg aaagattagt
     8221 taaagaagat agattatcaa caattcatat agaagaatat gaatgtgaag ctagagatac
     8281 aaaactaggg ccagaggaaa taactagaga tatacctaat gttggagata gtgcaattaa
     8341 gaatttagat gacagaggta taataagaat aggtgcagaa gtagactcag gtgatatatt
     8401 agttggtaaa gtaactccaa aaggagaaac tgaacttact gctgaggaaa gattacttcg
     8461 tgcaatattt ggagaaaagg ctagagaagt tagagatact tcacttaaag tgcctcatgg
     8521 tgaatctggt ataatagttg atgtaaaagt atttactaga gaaaatggag atgatttatc
     8581 tccaggagta aatgaattag taagatgcta tatagctaaa aagagaaaaa taaaagttgg
     8641 agataaaatg gctggtcgtc atggtaataa aggggttatc tcaagagtat tacctgaaga
     8701 agatatgcca ttcatggaaa atggaacacc actagacata atacttaacc cacaaggtat
     8761 accatcacgt atgaacattg gtcaggtttt agaagttcat ttaggacttg ctgctaagac
     8821 attaggatgg tatgtagcaa catcagtatt cgatggtgct aatgagtatg acataatgga
     8881 tgctcttgaa gaagctgggt atcctagaga tggtaaatta actttatatg atggtagaac
     8941 aggtgagtct tttgacaaca gaataactgt tggatatatg tactacttga aactacatca
     9001 cttagtagat gaaaaactac atgcaagaag tactggacca tactcattag ttactcaaca
     9061 gccactaggt ggtaaagcac aatttggtgg acaaagattt ggagagatgg aggtttgggc
     9121 tctggaagca tatggagctg ctcacatact tcaagaaata cttactgtta agtctgatga
     9181 cgttgtaggt cgtgttagaa catatgaagc tatagttaaa ggtgaaaata tacctgaacc
     9241 aggaatccca gaatcattta aggttcttat aaaagaactt caaagtttat gcttagatgt
     9301 aaaagtattg acagatgaag accaagaaat agaagtaaga gaatctgtgg atgaagatga
     9361 tacaataggt gaatttgagc tagatgttgt aaatcatatg ggagaagttg aagaaagtaa
     9421 tataatagaa gaaattgaag atgattttgc agaaaatgca gaagatgagg atatagagaa
     9481 tttagaagaa ttcactgaag atgatttatt tgaagaagaa atagattttg atagtgatga
     9541 ctttgatatg taaatttaat taatgtaaat ctcaaataga agggagagaa ctccttgttt
     9601 gaattaaaca atttcgagtc gataaaaata gcattggctt ctccagaaaa aataagacaa
     9661 tggtctaggg gagaagttaa aaagccagaa actataaatt accgtacttt aaaaccagaa
     9721 aaagatggtc ttttctgtga aagaatattt ggaccacaaa aagactggga gtgccactgt
     9781 ggtaaatata gaagagttag atataaaggt gtagtttgtg atagatgtgg agtagaagta
     9841 actaaatcaa aagtaagaag agagagaatg ggacatatag agctagcggc tcctatgtct
     9901 cacatctggt acttcaaagg tataccaagt agaatgggac ttttacttga tatgtcacca
     9961 agatcgttag aaaaaatatt atactttgcc tcatatgtgg tagttgatcc aggagagact
    10021 ggattaaatg aaaaacaatt gcttacagaa aaagaataca gaactgctct tgaaaagtat
    10081 ggatatactt ttactgtagg aatgggtgct gaagctgtaa agacattact acaaaatata
    10141 gatttagagc aacaaagtaa agacctaaga gcggagttaa aagatagtac agggcaaaag
    10201 aaagttagaa caataagaag attagaagtt gtagaggcat ttaaaaagtc tggaaataaa
    10261 ccagaatgga tgattttaga tgcaatacca gtaataccac cagatttaag accaatggta
    10321 caacttgatg gtggaagatt tgcgacttca gacctaaatg atttatatag aagagttata
    10381 aatagaaaca atagacttaa aagattatta gagcttggag ctccagatat aattgtaaga
    10441 aatgaaaaaa gaatgcttca agaagctgtt gatgcattga tagataatgg tagaagaggt
    10501 agacctgtaa caggacctgg aaatagacca cttaaatctt tatcagatat gttaaaaggt
    10561 aagcaaggtc gtttccgtca aaacttactt ggtaagcgtg ttgactactc aggacgttct
    10621 gttatagttg ttggaccaga acttaaattt tatcaatgtg gacttccaaa gaagatggca
    10681 ttagaattat tcaagccatt tgttatggat aaattagtta aagaaggtta tgcacataat
    10741 ataaaaagtg cgaaatctat agtagaaaaa gttaagccag aagtttggga tgttttagaa
    10801 gatgttataa aaagtcatcc agttcttctt aaccgtgcac cgactctaca tagattaggt
    10861 atacaagcat ttgaaccaat cttagttgaa ggtaaggcta taaaactaca tcctcttgta
    10921 tgtacagctt ataatgcaga ctttgatggt gaccaaatgg cagttcatgt acctttatca
    10981 gtagaagcac aagcagaagc aagattctta atgctttctg taaataatat acttgctcct
    11041 aaagatggtt cacctataac tactccatct caggatatgg ttttaggttg ctattatcta
    11101 acaatagaag cccaagatgg agctaaagga acaggtatgg tatttaaaga ctttaatgaa
    11161 ctattacttg cttattacaa taaatcagtt catctacatg cattagtgaa acttaaggta
    11221 acacttgaag atggaagaag ttcattggtt gaaagtactg ttggtagatt tatatttaat
    11281 gaaaatatac ctcaagactt aggttttgta gatagaaaag aaaatccatt tgcacttgaa
    11341 gttgatttct tagcagataa aaaatctctt ggtaaaataa tagataaatg ctttagaaaa
    11401 cacggaaata cagaaacagc tgaattacta gattatataa aagctctagg atttaaatat
    11461 tctacattag gtggcataac agttgctgtt gatgatatga gtgtcccaga agaaaagaaa
    11521 gtatttatag ctgaggcaga agctaaagtt gataagtatg aaaaagcata cagaagaggt
    11581 ctaatctctg atgaagaaag atatgaaaaa gttatagaga catggacaga aacaactgat
    11641 aaagttactg atgctcttat gggtggacta gatagattaa ataatatata tataatggca
    11701 cattcaggag ccagaggttc taaaaaccaa attagacagc tggcaggtat gcgtggtctt
    11761 atggcaaatg catctggtaa aacagttgag ataccagtta aatctaattt ccgtgaaggt
    11821 ttatcagtac tagaatactt tacatcttca catggtgcca gaaaaggtct tgccgataca
    11881 gctatacgta cagctgaatc tggatattta acaagaagac ttgttgatgt aagtcaagat
    11941 gttattgtaa gggaaataga ttgtggaaca gaagatacta cagagattta tgctataaaa
    12001 gaaggaaatg aagttataga agagatatat gatagaattg taggaagata tactatagac
    12061 cctatattaa atcctgaaac tggagaagtt atagttgagg ctgattcaat gatacaagaa
    12121 gatgaagcag aaactatagt agctttagga attgaaaaaa ttaggataag aacagttctt
    12181 aactgtaaaa ctaatcatgg agtttgttct aagtgttatg gtagaaactt ggcaacagga
    12241 aaagaagtta atataggtga agcagttggt ataatagcag ctcaatctat cggtgagccg
    12301 ggtactcagc ttacaatgcg tacattccat actggaggag ttgcaggagc tgatataact
    12361 caaggtctac caagggttga agaattattt gaagctagaa aaccaaaagg attagctgta
    12421 ataactgaag tatctggtag agttgaaatt gatgaaacag gaaagagaaa agaagtaaat
    12481 gtaataccag aagaaggcga gactcaaaca tatgtaattc catatggttc tagattaaaa
    12541 gttaagcaag gtcaaatgct agaggctgga gaccctctaa cacaaggatt tataaatcct
    12601 catgatatag taagagtaaa tggtgttaag ggagttcaag aatatatagt taaagaagtt
    12661 caaagagtgt atagacttca aggggttgat gttaatgata agcatataga agttatagta
    12721 agacaaatgc tatctaaagt aaaagttgaa gacccaggag atacagattt attgccaggt
    12781 ggatatgaag atgtattaac attcaatgaa tgtaataaag atgctataga taaaggtcta
    12841 agaccagcag ttgctaaaag agttttactt ggtataacta aagcatctct tgcaactgat
    12901 tcattcttat cagcagcttc tttccaagaa acaacaagag tattaacaga ggcagcaata
    12961 aaaggtaaag aagaccactt aataggactt aaagaaaatg ttatattagg taagttaata
    13021 ccagcaggaa caggaatgaa gaaatataga aatatagctg ttgaaaaaat tgaagattaa
    13081 tagcatatag agtaaagaaa tcttgacaac cattatttgt ggtgttaaaa tagtaaagtg
    13141 tgttgtttaa aagtacacta agggcttgaa caagttctta gtgactttta gatggcaata
    13201 aatgagaact aaaaagcatc aagctagaaa agtgctaata tcacatattt atgtgttata
    13261 gacattaaat tgtaagtaac gaatgttaca atcagtcagt atgaatattc atattgatgt
    13321 agttggcggt cactgataag atcgaaaatt tggaaggagg tgcagatgat gccaacaatt
    13381 aaccaattag ttcgtaaaag tagaaaagcg ctagagaaga aatctactgc tccagcatta
    13441 caaaaaggtt acaactcttt aaataagaaa gtaactgacg ctagtgcccc acaaaaaaga
    13501 ggggtttgta cttcagtaaa aacagttact cctaagaagc ctaactcagc tttaagaaaa
    13561 gttgccagag ttagattaac taatggtata gaagtttctg cttatatacc aggagaagga
    13621 cacaacttac aagagcatag tgttgttctt ataagaggag gaagagtaaa agaccttcca
    13681 ggggttagat accatatact aagaggtact ctagatacag caggtgttga taagagaaga
    13741 caatcaagat ctaaatacgg tgcgaagaga cctaaagaag ctaaaaagta aaaacaatta
    13801 atgattgatt atagttcggt gctttaaata tatatatatt tacggcatca cggcattaaa
    13861 tgatttatgt cgagtaccta tgatttaagt taaataatta aggagggaag cgaaatgcca
    13921 agaaaaggta atattccaaa aagagaagtt ttaccagatc ctatgtatgg gagtaaggta
    13981 gttactaaat taataaataa tctaatggta gacggaaaaa aaggaaagtc tcaaagaata
    14041 gtttatgatg ctttcgcaat tgttgctgaa aagacaggag aagaagcttt agaagtattt
    14101 aataaagcaa tggataatat aatgcctgtt ttagaagtaa aagcaagaag agttggtggg
    14161 gcaaactacc aagtgccaat cgaagttaga cctgaaagaa gacaaacatt aggtttaaga
    14221 tggctagtaa aatatactag agctcgtgga gaaaaaggaa tggttgaaaa gctagctaaa
    14281 gagataatgg acgcagctaa caacactgga gcttcagtta agaaaaaaga agatactcat
    14341 aaaatggcag aagctaataa agcatttgct cattacagat ggtaggatat atagatatct
    14401 tataaaattg aggatatcat aaggaggata accatggcta gaaagtttcc tttggaaaga
    14461 actaggaata taggaatcat ggctcatata gatgcaggaa aaactactac tacagaaaga
    14521 atcctgtttt atactgggca aactcataaa ataggagaaa ctcatgaagg agcttctcaa
    14581 atggactgga tggagcaaga aaaggaaaga ggtataacaa taacttctgc cgctactact
    14641 gcttcatgga aagaccatag aataaacata atagatactc ctggacacgt ggacttcaca
    14701 gttgaggttg aaagatctct aagagttctt gacggttcag ttgctgtatt ctgtgcaaaa
    14761 ggtggggttg agcctcaatc agaaaatgta tggagacaag ctgaaactta tggagtacct
    14821 agaatagctt ttgtaaataa aatggatata ttaggtgcag atttctacaa tgttgtaagc
    14881 atgatgaaat ctagattaaa ttctaatgca gttcctatgc aattaccaat aggtaaagaa
    14941 gattcattta taggtataat agacttatta aaaatggatg ctgttatata caaagacgac
    15001 ctaggggttg aaatggaaga aacagatata ccagaagata tgaaagaatt agctgctgag
    15061 tggagagaaa aattagttga atcagtagct gaaactgatg aagaattaat gatgaaatac
    15121 cttgaaggtg aagaacttac tatagatgaa ttaaaagttg ctataagaaa agctactata
    15181 gcttgtgaaa tgaacccagt tttctgtggt acagcttata gaaacaaagg tgttcagtta
    15241 gtaatagatg cagttttaga ttacttacca gcgccaactg atataccagc tataaaaggt
    15301 atattagcag atggagaaga agctgaaaga cattcttctg atgaagagcc attctctgca
    15361 ttagcattca aaataatgac tgacccattc gttggaaaac tagcgttctt cagagtttat
    15421 tctggaacat tagaatcagg ttcttatgtt ttaaatgcaa ctaagaataa gagagaaaga
    15481 attggtcgta tattacaaat gcatgctaat acaagagaag aaataactaa agtgtatgct
    15541 ggagatatag cagcagcagt aggattaaaa gatactacta ctggagacac attatgtgac
    15601 cctgctaatc caataatact tgaatctatg gaattccctg agccagttat atctgttgct
    15661 atagaaccaa gttctaaagc agctcaagaa aaaatgggta tagctcttca aaaattagct
    15721 gaagaagacc caactttcac agttaagact gaccaagaaa caggacaaac tataatatct
    15781 ggtatgggtg aattacactt agagataata gtagatagat tattaagaga atttaaagta
    15841 gaagctaaag ttggtgcacc acaagttgca tatagagaga ctataactca accagttgat
    15901 gttgaataca agtactctaa gcaatcaggt ggtagaggac aatacggtca cgttaagatt
    15961 agagttgctc ctcaagagcc aggtgaagga tacaaattca caaacaaaac tgttggtgga
    16021 tcagtaccaa aagaatatgt tggtccagtt gacatgggta tacaaggagc tatgcaatct
    16081 ggtatagttg ctggatatcc agttgtagac gttgctgttg agttatatga tggttcttac
    16141 catgaggttg actcttctga gatggcgttt aagatggctg gttcaatggc attcaaagat
    16201 gctatgaaga aaggtaatgc agttctttta gagccttact tcaaagtaga agttgttact
    16261 ccagaagatt acatgggaga tgttatgggt gacttaaact caagaagagg attaatccaa
    16321 ggtatggaag ctagaagtgg tgcacaagtt ataaatgcat tcgttccact ttcagaaatg
    16381 tttggatact caacagacct aagatcttca actcaaggtc gtgcaactta cacaatgata
    16441 ttcgatcact atgagcaagt tccagcttca gttgctaaga aaatagctga aggaaaataa
    16501 tttaaaaata attaataata taaatataaa aaataatcag ctagatgaga gatatctctc
    16561 atctagcaca agat
//
LOCUS       DAELNY010000076        15887 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.076, whole genome shotgun sequence.
ACCESSION   DAELNY010000076 DAELNY010000000
VERSION     DAELNY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.076"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(74..262)
                     /locus_tag="KPE55_002082"
     CDS             complement(74..262)
                     /locus_tag="KPE55_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493146.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            1203..1598
                     /locus_tag="KPE55_002083"
     CDS             1203..1598
                     /locus_tag="KPE55_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493147.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            1641..3740
                     /locus_tag="KPE55_002084"
     CDS             1641..3740
                     /locus_tag="KPE55_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG0493148.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(3965..9175)
                     /locus_tag="KPE55_002085"
     CDS             complement(3965..9175)
                     /locus_tag="KPE55_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG0493149.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKALIGNVTEEKIRNILKKAKEDLDSQEKDYDGYKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSSKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(9190..11685)
                     /locus_tag="KPE55_002086"
     CDS             complement(9190..11685)
                     /locus_tag="KPE55_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0493150.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDERDGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIDR
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFAKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(12322..12609)
                     /locus_tag="KPE55_002087"
     CDS             complement(12322..12609)
                     /locus_tag="KPE55_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493151.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(12629..12904)
                     /locus_tag="KPE55_002088"
     CDS             complement(12629..12904)
                     /locus_tag="KPE55_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493152.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(13537..13716)
                     /locus_tag="KPE55_002089"
     CDS             complement(13537..13716)
                     /locus_tag="KPE55_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493153.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(14001..15467)
                     /locus_tag="KPE55_002090"
     CDS             complement(14001..15467)
                     /locus_tag="KPE55_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0493154.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKIVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         5260 a         2792 c         1751 g         6084 t
ORIGIN      
        1 actttttaca gataattaaa aagttttatc ataaataaat cataactaga catcctagta
       61 aatcaaaatt aatttattct tttttattag aatttttgat aacagtccct atacatatgc
      121 caattatcat tccaaaaatt agaccataac acattgtgat acctacattt cctttagaaa
      181 tacctatagc acaacctata gccattccaa gtatcatgcc tataaacata catataataa
      241 ctaaattgtt gtttttattc atagtttttc tccattcaat atattttaaa ttgcctagct
      301 cgccttctta gcttaaaaca aagaaatata aattaacttt ctttcattct aatatctgct
      361 tgtataaatg tcaaacaaca catttcttgt cataacctcc tctttttgaa gtaagtacaa
      421 tctcccagac gcaataatat caatttccta aaaccattta agtaactttt atatttacat
      481 aatatttaca atcataaact atattgttat tcatttatac atcataaaca ctccctccta
      541 atcattatta tacttaaaat aatgttcatt tataataata tatctaacgc actttaattg
      601 aaaaaactaa aactttgcct ttcattccca tatataaata agtatacttt ttgatgctct
      661 attacaaatc aactaaatta attgatataa attatcattt tctgttgaaa ataccattcg
      721 aacatgctat acttaattgc ataaatggta ataaatgtca tatgatttta caaatctatt
      781 aataacaatt attactttta ctgtattcta aagtttttgg actaaatatc aagaactttc
      841 taaaatacaa agtacaaaaa atttatataa gttataaatt tatatgaact agataagctc
      901 aaacaatatg ttatgtataa actgtgtagt attcggttat aaatttatat aaattatata
      961 agctagataa agtattacct caactatata agctacatat ataaattaca tacataagct
     1021 atataagaat tatttcaact atataaacta cgcatgtaaa attcatgtaa atttactaat
     1081 ataaatcaaa taaaattact aaactctcta aactaatttt gtaaaacata ctctattttt
     1141 aacatttgtt accttataca taagtacaaa caaaatctaa ctaaatgaaa ggataatgta
     1201 aaatgaatat tttaaaaatg aaaaaaacag ggatattttt aggaggagta ctttttggta
     1261 ctgtaggtgt taagatactt tcaagtgatt gtgttaaaaa aacttgcact cgtgcaacag
     1321 caggagcatt gaaagttaaa gattctgttc ttgaaacaac tacgaaaatt caagaaaatg
     1381 tagaagatat tttagctgaa gcaaaagaaa tcaacgagca agaagactta tctacatcta
     1441 aggtaaacgt tgtacaagat actcttaaat gcgaacaaga agttcttaaa actgaaacta
     1501 taaaaactgt agaagatact aattctaata gcattaaatg tgaagaaatc aaagataacg
     1561 taattgaaga aaaaaatagt acttgtacaa cagaataaat ttaatgatta ttatgattaa
     1621 taatatcagg aggaagtaaa ttgaattttt atataaaaca cgaaataaga ggtcgtatac
     1681 gctttgactt acaacttggc aaattaaaca acaagcagtc cgatactctc ttatattatt
     1741 taacttctat aaatggtatt accaaagcaa aagtttacga tagaactggt gatgcagtca
     1801 tttattacac tggtgaaaga gaatacataa taagagaaat cactaaattc agttttaaca
     1861 atagcaaaat agaaaaccta gttcctgaac ataccagcag ggaattaaac aattattata
     1921 gagacaaatt agcaaataaa ctgctagtca aaagtatctc caaaatattt tttccaaagt
     1981 ccttaagatt ttttttaatt ggacttcatt ctataaaata ttttaaaaaa ggtctctctt
     2041 ccctgctaaa taaaaaaata gaagtttctg ttttagatgc cactgctata ggtatttcta
     2101 tttttagaaa agatatcaat acggctggtt cagttatgtt tcttcttggt atcggtgaac
     2161 tattagaaga atggactaga aaaaaatcta tagacgactt ggctcaaagt atgtcactaa
     2221 acattgaaaa agtatggtta agaaaaggtg acactgaaat cttaattcca atttcagaga
     2281 taaaggaagg agatttagta agtgtaacta tgggaaatat gattccattg gatggagtta
     2341 tagtatcagg agaaacaatg gtcaatcagg catcacttac aggtgaatcc cttgcagtta
     2401 acaaaaaaga aggctcttac atatatgctg gaactgtaat agagcaaggc aatattgtta
     2461 tgtgcgtcaa ggaaaaagct ggtactacta gattccagaa aatagtaact atgattgagg
     2521 aatctgaaaa actaaaatct tctgtagaga gtaaatttga acatctagca gatacattag
     2581 ttccatacag ctttttagga agtatattaa cctatgcaat tactagaaac cctataaaat
     2641 cactctctat attaatggta gatttctctt gtgcattaaa actctctatc cctatttcag
     2701 tactatcagc catgagagaa tgtaacaata acaacattac tgtaaaaggt ggaaaatttc
     2761 ttgaaggtgt tgcatgtgca gatactattg tatttgataa gacaggcaca ttaacaaaag
     2821 ctcaaccaac tgtttcagat ataattacat tccaaaatta taataaggag gatatgttaa
     2881 gacttgcagc ttgcttagaa gaacacttcc cacactccat agcaaatgca gtagtttatg
     2941 aagctgaaaa acaagggctt agtcacaaag aaatgcatac agaagtagaa tatattattg
     3001 ctcatggcat ttcaacaaaa gttgatgata aaaaggttgt aataggtagc aaacattttg
     3061 tatttgaaga tgagaagtgt actatcccag acggtgagga ggataagtac aataatctat
     3121 cagatgagta ttcacaccta tttatggcaa tatcaggtaa attatcagca gttatttgca
     3181 taaatgaccc tttacgaaaa gaagcaaaat atgtaatttc aaatttaaga gaatgcggaa
     3241 taaaaaagat tgtgatgatg actggagata gcgaaaaaac agctaaatct atagcaagta
     3301 aagttggtgt tgatgaatat tattcagaag tcttaccaga agacaaagcc aattttgtta
     3361 aaagtgaaaa actcaaaggt cgcaaagtaa ttatgattgg tgatggaatc aatgattctc
     3421 ctgctatttc agaatcagat gtcggaattg caatgagtga aggtgctgaa atagcaagag
     3481 agatttcaga tattaccata tcagcagata atttgaataa tttaatcgtt ttaaagcaga
     3541 ttagtaataa gcttatgaaa agagttgatt taagttctaa gtttattatc ggctttaatt
     3601 taggtttgat tttacttggt gtaggtggat ttgttagacc aagtacatct gcctttttac
     3661 acaacgcatc tactgtcgga ataagtttaa acagtatgac aaatttattg gaaaacacca
     3721 atacttataa ctaccattaa tctccattac ataaaacata tgatataaaa aatttataat
     3781 tcatcttttt aaaaaaatat gtcttaaaat tagctttttt ctaatcaaaa ttatataaag
     3841 catactttac aaattttaaa ctcaacttat ttctattttt tacaaaaaag gagatgattc
     3901 aaattttcat tttgaaacat ccccttttgt aaatttcttt aaaatttata tattaattat
     3961 attttcaacc aataattgta ttttcaacca atctttcttc atagcatttt aaatcatttt
     4021 gctttagata attaaattca gactctacat aaataccaca gcctaccaat tcaattgtca
     4081 aattaggtat attttccttt agtatactag tctgctcata aataattctt tccaataaat
     4141 caacttgtaa tatagaaaaa tgcttcataa tctccccatc actatctata tctttcttga
     4201 tttcatacca actctttgac aaaattccct tcaaatcaat ataccctaga ctcctcaata
     4261 tatctatact atcgctccat gcatacttac taggtttatc aaagtatgac tctagtttct
     4321 tcttctgctc atttgtaagt gaatcataag aaagcttcct attaaaatgt gccttcaaag
     4381 aaatcttagc catagaatat ggacttctac cagagtttaa tcctgcattt ttgaactttt
     4441 caattccttt atcttctact gaagaccata ttgagtttat tgaatctgaa tcattgtatt
     4501 taacatcaag accatgtgta atatttatat agttttgtgg aaatacatat tgataataga
     4561 catctttttt atcatcatat agagagaagt atccactatc atataaatga atatgcacct
     4621 ttacttttaa actatcctct aaaatatttc gtctaaggtt cgggatattt gcatacatgg
     4681 ttacatattt tatggcatca aagtttttat ttaatggaac ttcaaaacta atttcatctc
     4741 ctgtcaaaag ctttttcact tcttcttcat tacaatcata tagagaatat gtattttcaa
     4801 atcccacacg tgatttccca tctaaactgc ctgttaatgc taccttcaaa atatggttat
     4861 ctgttttttg ccattggtaa gcatcttggt caagaaactc tctaatctgt ctattttgct
     4921 ctttcgcctg atttataacc tcgttttcat cttctgtaat ccctacatgg catctattta
     4981 atatatttac tatattgctt ccaaatggga atgtccatag ttgaaaagct gctgcctcaa
     5041 cctgacacaa gtatctaacc tgatttaatt caatttgatg gataagtttt ttctgatatt
     5101 cattcatctg attaccacta tctgacattt gataaatcaa ttctcccatt gcaagattat
     5161 cttttttctt atcaatctta tcagtaatga taatttgagt ttccatcata tcctgtactt
     5221 ttttaaatat tatagaaatc tcctctgaat tttccatttt taagcttcca aacttcgtgc
     5281 ttaattcagt taaaacttcc ttcatcttta atttacttat ctcattttca aaaatatctt
     5341 gatttttaac tgtactatcg aatttttcta caaatggtat aacagattct ttcaaagttt
     5401 tatctttaaa ttccaccatt tgagtattca attcatctaa tttcttaata ttattttctt
     5461 tatctttaat tgcctcttta actacctcta ttttactctc aaaactagat tttacattaa
     5521 gacaattatt aacaccacta atcgcagtat ttataagaga acaagcagaa cttccaatac
     5581 tcaatgcatt tttaatttta gatgtctttt gctgtctttc aatagcttgt ttacttttct
     5641 ctagtgcagc taacttatct ttttgctgtt tattattgta atttgcctcc aacataccta
     5701 tttctttttt ctggattttt gtgtaaattt ccttatactc gctttcactt aacccctctt
     5761 ttgctctttg aatatcgtca gctttgtctg catagttatc atcatctata gacttaacta
     5821 taatctcctt aatttggtca aatttagttc ttctttcttc atcaacacct gccaaattat
     5881 aagtcatttt atctaaagca ttttttctat cttcacttag ctgttctcca agtacaaatc
     5941 tcataccatc agaatccaat ttaaaatctg aaaaattaac catcctgtta gtccatttgt
     6001 attcctcttc tttagatgaa agattttcct tcaaagattt tgcactgtca aatgccccta
     6061 gacaagcttg accaacacca ctaaatgcag tcagtaaact caatgcgctt tgtattccag
     6121 aaagaattaa ctgtgttttc atttgttcct caagttttgc ctgtttcttt tttagttcct
     6181 ctatgtcttc ttgttcatct tctttcatct ttataatttc ttgaatcaac aggttcattt
     6241 ttgcattaac agaaagcatt ttattctcaa gttcattttt taaaacctct atttgttgat
     6301 ttccagattc aatttgagtt aggatacttt ccttatatgt attgaaatat ccttctttta
     6361 actcatcttt cttcccttgt tccaattctc ttaaggtctt atccagataa tctgtcaaag
     6421 aacgaatatc tgtaacacga tttcctccaa tttcactttc ttcaatcatc tgcacataaa
     6481 atgctgtctt ctgctcaagt gcaaataaaa tcatactcca ttcgctaaaa ttctgatttt
     6541 ttcctacaaa agattctatt ctatcagact gcatcaattt atttataagg tattcataga
     6601 tttcctttgc ttctttataa aattctttct ttgtattcat atcatccaga gaaagtactg
     6661 ctttattttt cttctcaaaa ctatagtact tctgaagtat ttctctattt aattgcatct
     6721 ctattttctc aactgcatga aaaatttcat aaatatgtga aagttctaaa atatcagctt
     6781 ctaaaattcc tactgcacac aacagtctaa gatttctata acctttaatt ccagaaaaac
     6841 caaatctctc gaaaaattca tagaagaatg tatctattga caaagaatac tcctgcttta
     6901 acaattcctt ttcttttagt gcatctccag agtgaccagc ttttccctct gctccggctg
     6961 aacctgaatc ttcacgtttg ctaattgtcc agtccttatg ttcttcataa agagtattta
     7021 atttctctcc atcatctgct gcttgcttct gatttaaagc atgattaaac acatatctat
     7081 caaaataata gtagtttcta tcactattat ccattggagg agaggtacct atatatctta
     7141 ttgtttcttc atatatacct ctaattccac caagacctcc tgcaccacca gctcctccag
     7201 agcctccttt tcctgctaaa ccatgatttc catctttttt acttatagca ctttttaata
     7261 aaggagcttt tccaaggaga taaacctgtc cagacttgct tccaagacct gcatttccac
     7321 cattacctcc tgtgcctcca gttccacctt gttttccttt ttctgctttt acaacatagt
     7381 attttgtacc tttaacatta gcaaattctt ttgactcttt tttagaacct attttagcat
     7441 ttgccccagc ttctccagtt atccctgtat ctcctttttt tccatcttgt ccatttccac
     7501 cacgtccacc atctacgtaa agtttaagtt ctcctctttt ttctatttct ttaaaagata
     7561 atatgatatc acctgaagta cctgcattat tgccatttct tccattcatt ccgtcgtaaa
     7621 tagaactttc tccaaccaat ttttctgcat ccttaccaga tacgttgatt gaataagtcc
     7681 cttctggtat atgtaatttt tctgtaagta gtacaatatt cttacctgag aagacttctt
     7741 tctccatatt acagtcaatt ttcaactcac aatctccata catatatata gatgacaaaa
     7801 tattaagttg ataaccagat attattttag tcaaatccga taaattcact ttaggtatag
     7861 gaatatatat agaaagtaca ttttctttct catatgattt tatatatgta caactttgaa
     7921 tcttctctat cctatactct gcatcttcaa gctttttggc ttcttcttcc ctatattctt
     7981 cttctgctat ccaatatttg atatctttta atgtatcttc taaagtattt tttagcaatg
     8041 attttaacaa ctcacttaga ccaacaatat gagtctcact acagtaacta tttaactcta
     8101 aaagaagacg tgtttcttct aatatattca ctttctgtac gccatcacca tatgaaagtt
     8161 cttgtacaaa ctccataagc tctttcatat tattagtcct actacataaa tctgtataca
     8221 tcttatctaa ttgaatcttt ttatctttta gactatttat agaagataga atggaaatag
     8281 aatcttccat acctttttta acactttcta tataaggaac taaaagtttt tctaggtctt
     8341 tataggttac atcaacagct ttttgtattt ctattttaca agaagcagta agcatcaatc
     8401 ccaaatctgt tttttcaaat gtttcaggta gatttttcat ttcattcaac aacttttctg
     8461 gctgtttatc tagtggatta cataatttaa tagatacacc atctgataat gctacattta
     8521 ataagtacac taattcctta taaccgtcgt aatctttctc ttgactatct aggtcttctt
     8581 ttgctttttt caatatattt cttatttttt cttctgttac attaccgatt agtgctttag
     8641 gtattttatt gataagaaag aagaaatttt ctttatactt tgcataatca ccaagcatat
     8701 ctttcaaatt tttgcctgtt tctattaata atgtatgtct agagtccagt aaggattgat
     8761 agtctaaaat aactaccatc cctttcacct ttgaagatgt agaaatagtt tcccttacaa
     8821 gcatagcacc agcaatcata tactctgctc cacggttatc aaaaaaacct gggcaatcac
     8881 ataatgaaaa atctctattt gcaatatcta caacttctgg acataaagtc ttagaatata
     8941 caccatttcc aatttcgaca gctgaatctt ttgcagttat atatatttgt ccagtctctt
     9001 catccgtttt ttcttccatc acacagcctt ttaaataatt tatacaagta ctctttccaa
     9061 cgcctgaagc acctaggaat aacaaaattg acttttcttt taagtcatat tttctaagct
     9121 cgtctcttcc ttcttccaaa aggtttacga tttcactata aagtttattt gccatacatc
     9181 catccctttc tatatttcgg aacaatagcc ttgatatttt tctttaataa gattctcttc
     9241 tacctttgaa acagctgttg ataacttagt gtttggagca ttaaatcctc cattgtctcc
     9301 tctatctcca tattcagcgt attctcttgc tggtcctgtt ttaccttcat tccaacttcc
     9361 accactaaat tctatatgat atttaattat actatccaac ttataatccc aacttgtaaa
     9421 atatcctgta tagtacacag tcttatctat accacttgga gaaaatgaaa gtgtattgta
     9481 tccgtgatag ccaccacttc ctccatatcc acctgaacct ggtctaccat ttggacctgc
     9541 ttctccatct ttcactgtta tatcacaatg attaattatt ctattttttt gttcctctga
     9601 atctgaaatg aagtaaatat tacctttctt accagcattt ccacctcttc cagcatttcc
     9661 accagctcca cctttttcac cttttttgcc acttgtacct tttgcaaaat accctttaaa
     9721 catctgtccg tggaaataac ttgtataccc tgcatcagct ccattacttc cagaagctcc
     9781 tgtttcacca tttttcccat ctattccttt agaaccattt cctccatttg caatcagtgt
     9841 taaacgataa tttctaattt cttctttaat atataagtaa atattacctg aatctctacc
     9901 atcttcacca tttttaccat acatagaact atctacaagt tctccgctct ttccacttac
     9961 attaattttt acattactac ctacatcaag attgttacaa gcaagaataa tatttttctc
    10021 tgatgcaaag tccattccaa ggttatcatc catataaata gtatctttag caaagaacat
    10081 aaatgtagaa acattgtcat ctacttttct atttttgaaa tattccaata cactgctaat
    10141 tactaagttt ggaattaatg ctcttatttc aatacctttc ttatcattaa tattaattgt
    10201 ttttactata aatggattta aaatgtattt accaataaat tctctaatta tctcattacc
    10261 ttcttctcca tatgcactta atcttgttat actgtcttct aactcttcat tttgtgtaac
    10321 tgtaacattt gtcaaaattg aattaaaatc acttacacta tatacataat cctttttatt
    10381 tgcaacttcc attaattttt ctctatttgg taatgtaaat acctgttttt gcacttcata
    10441 agaaactaaa actttttcta aattctttct tattataatg aagttttgtt ccattatctt
    10501 cttacttgcc ttttctataa taaattccat atgcacagaa ggtaataaaa tttccccagt
    10561 tccatgttct gtatatttac ttaaagcatc aattatatct aagtttcctt ttatcttatt
    10621 ttgtaatttc atgcaactat cttgtaagaa taagccactt tgtttggcta atacgttgat
    10681 aaatctgtct atagtaagac ttgttgtact ttccaaccag tttttaaata cttttacaac
    10741 ctcatcatat tttgctattt cttgaatcat tgttatactt ccaataatac tgataaaatc
    10801 ttctgtatac gccttaacat aatcttctag gctagatacg atattgtttt gaatttcttt
    10861 tatcaacgta tctaaaacta atcttgcatc tgcactaatt ggatactgga atgctgatgt
    10921 cttatcttta acttcatcta attctagtac ttgctttaat aaaccgtccc tctcatctga
    10981 tgcaagtggc ttacaaagat gaatattttt tccattatct tttagcaatt cttcaaataa
    11041 ccaagctttt tcctctatat tactgtctaa attttcgcat aattgttcat attttctttt
    11101 tataacatta tatatttgtt tttctgttct tcctaagaaa ctttttgaaa taaaaaaagt
    11161 tatactaccc ttaaaatttt ctttatgaag acacatattt aactgttgga acaggtctag
    11221 tacttttgta agtctagaat ctaatatatc tgcttctggt atcaatacaa ttactgcaca
    11281 tatagatgca gaatttttaa atacttcacc taatgaaact gctgcgctaa tatcatgggc
    11341 tactccccta ttgtcgctaa aacctgcagt atcacataat tttgcatcca ttccttctat
    11401 tggttttctc atatttacaa tgttcgggta taaagtttga gaaacagctc ctgaaccaat
    11461 ctctgccaca gggtctaagc aatcaatata tgatatacca gtatcttcat cttcgacttc
    11521 catcattaca catcctgcaa gatagttaat tgcagtactt ttgccacatc ctgtcccacc
    11581 aagaattaga attaaatttt gattttcagc cttatttatt gctacttttc cttcatttaa
    11641 taagagtgtt atttcttcta aaagttgatt gttattttct ggcatttatt ttcccccttg
    11701 gttatatatt ttatttaaat aaagtttaat cccttaagta gattacaaag tttttagact
    11761 atatttaaat ttgtttttta ttttttattt ttaataaatt tgcattttca ctaaatttgt
    11821 attttcactc aataattatt cttctttgtt tattaaaata actgtgttgt ataatataat
    11881 ctttaccaac acagttattc tatttaatgc gcttatttta ttgtatttta tgtaatacag
    11941 ttgtttttta tattatatgt ccattatttt cctatggtta ttacatatag atttttaatt
    12001 atatttgtac cattaattta aacattccta tactactgct taagttttac acctatacca
    12061 gcttgaatat tttattttta tttgcttaaa tagttcaact atactggttt aaataggtta
    12121 tctttatttg cttaaatagt tcaactatac tggtttaaat aggttatctt tatttgctta
    12181 aattagttca actatactgg tttaaatagg ttatctttat ttgcttaaat tagttcaact
    12241 atactggttt aaataggttt cctttagttt aaataattca cctataccta cttgaatagt
    12301 tcacttttat ttgcttaaat tctataccaa tactggctta aacattatac taccatgatt
    12361 gtattgtcta tcttcaacta caataaaaac aggttctaaa tctttagtag atgttatttt
    12421 taattctaaa aaaacattat cagttggtaa tatttttaca tcataattat ttaaattatt
    12481 attagtgata ctattaaata catcaccttg acgttcaaaa aaaacattat caactattac
    12541 aaaataaaca tcttcttttc tactattttc aacctctata gtattttgtt tagttacatt
    12601 tagtctcatc ttccacctcc tattaatatt acttaattat attaatactt gtaattgttg
    12661 cattatgttc atcacaagga agaggtttaa aaacaactat ctctgctaaa taattctgtg
    12721 gaggatttgt aactgaagta gcattgcaaa taaatttaaa atttgttcct gctacaactt
    12781 gagtagaaac aataagtggt tcataaccaa ccccttctaa cattcctaca gcttcattaa
    12841 atacttttaa atcttcttct tcgacagctc tgaattttga ccatccacca acaaaaagac
    12901 tcatataaaa acctccttta tatatatatt tatttatata tagtagtacc aattttctca
    12961 atattcaata ttttctgtgt aactggtcaa tgaaaatgca taactggata aaaatactta
    13021 taaaataact taactttaca tataaatagc ttactttaca tgtaataaga ttattctaca
    13081 atatgaaata catcttgttt tgaatatatt ctaaatacaa taatggatat tttcaattat
    13141 tatccttaga aaatatccat tacttaactt gttttattta aaatcacata gagactttat
    13201 tatttttttt attgcatgaa tagctatttc aggtatcata aggtgatata ccccctcctt
    13261 gaaaccacaa aaaacatgac aaactttacg aatcttggct taaaaaacta caggtacatt
    13321 ccttatttgt ggattggagt agccataaca agcaaatttt acaattttta tgatgacaaa
    13381 atcaataaaa tcgcttagta tataacacga aacactactt gttgctatta agaatagtta
    13441 gataggaagt atgcattata ccaataaagg aatcaataaa gaaagttaaa acatccctac
    13501 aaatcaaaaa tctgttacat tttctatatt taaacgttat ttacttacaa ttggagaaat
    13561 aacaggtttt ccttctcggt caagtaaagg cgtaaatccg gctgcatttc cataatagtg
    13621 gaatacatag ttcacacctg tttctttatc cacccaaatc tccgttgcat ttatttttcc
    13681 ttgtgaatat actttttcaa atctatcttt tcccatttta taatccccca tatttttgat
    13741 tggtctatac cttcttcact taagtcgaaa aaatacataa cttatttttt taattctaat
    13801 atgaacacgt aaaaatgtca aacagcttat ttaatttcat aacttcatgt tactgttcgt
    13861 aaccttgttt gtttacttac actacattac agcaccattt taccctttac atgattgtat
    13921 tgcacacagc aaaaatgctt actttattgg atttttcaac taaaaaaagg tttttttctc
    13981 cacagaacat ttactatttt ttataatata tctaatttta ctctcatatc aacatttaat
    14041 gaagatataa cctttttcaa atattgtaaa gactccttaa aaatattaga aaaagattct
    14101 gcaaatctct caacatctcc tttttttaaa tacctaagta tctcaaaaca tacatcaaaa
    14161 cgtgttttca aaatttcatt atattcttca tgaaaaaatc tctctaaata gattccaaat
    14221 atcatatatt gttttaaatg caaaaatgtt tcctgtaata cataaatttg tgtataatct
    14281 actatcttac aaagtaaaac aaaatctgga taaaccttac actcttcaca attattttgt
    14341 aagtacctta aatgagtttc tattttatct acagctatca aatccatttt atttcctagt
    14401 aaaagtacta catcctcaag agtaatcgta acaaactcca tagcatccaa aagcaaactc
    14461 aaccttttag ctccttcttc tgtatttata taatcatcat catattcaga aaaaagtgtt
    14521 acatatgttc ccttgccatt aattgtttta gcaattccca attcatttag ctcattatag
    14581 gcacttctaa ctgttggaag tgatacatta tatctattaa ttatcgaagt aattgatggt
    14641 agacaatctc ctattttaat attgccttca aaaatttgac aaattacatc tgaaacaatt
    14701 aagtcatata aataacattt ttccaacgtt acagaattaa aactacttgt atctatactt
    14761 tgtggagcac gattttctac tattttaagt atggattctc tacaagcttt atacatatat
    14821 gttaatagtt ttttaaattc aattagattc ccgtccaatc catataagta agtttttctt
    14881 acctgctcta tgtatttatt acgaaaaaag tctatacaac aattatccaa gttatcgaaa
    14941 ttataggcca attgtgaaat aatgacatca tgctgaataa tctcacaaaa atggtgaatc
    15001 aggaaattat ttgttttact tattagttta tttatatatt ttagcctttg ttctttaaag
    15061 actaaataat tagaactatt tttttccata caatcgataa tctcgtttaa ttcttcaaag
    15121 tctttagatg taaatctttt aatattgtac atagcaatag aaggaaatat taaactaaaa
    15181 atcttatata tttctgatat ttgtaaaatc ttctcatata ataagtcaga ttctattgat
    15241 tgtttactcg tactctggat taaaactggt ctagaaccag cttttgtctc tataaagccc
    15301 ttttctgaga gcatttttat tacagttttt atagtatttt ttgataccat atattcttct
    15361 gacatgcttt ttatagaatc aagcctatct ccaatagtaa ttttaccagt ataaatttgt
    15421 tgaattatat gctcaaaaat aattaaatat tgtggttttc tcttcatatt tttcctcttc
    15481 gataaattag atttattgtt ttttgtaagg tatatttact tttatttaat agggcaaaat
    15541 agttgcttat tttttcctca taatctataa ttaatataaa ccatttatat atatggtcta
    15601 agttttataa tactgtaatg cagtaaaata gcatattgta gctatactac agcaaattaa
    15661 cataccattg tagtatatta attggaataa ttttaagtac ttcaaaattc cagcaaaaca
    15721 tatatgtgtt tttgatatta tgtatattcg ttaatagaat agctatactt gttttacata
    15781 ataaattata tgatacttgc tatatgtaat tactatattt aatacacata actatgatac
    15841 ttgctatatg taattactat atttaataca cataattatt atactta
//
LOCUS       DAELNY010000077        15827 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.077, whole genome shotgun sequence.
ACCESSION   DAELNY010000077 DAELNY010000000
VERSION     DAELNY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.077"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(176..1057)
                     /locus_tag="KPE55_002091"
     CDS             complement(176..1057)
                     /locus_tag="KPE55_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG0493155.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1513..2487
                     /locus_tag="KPE55_002092"
     CDS             1513..2487
                     /locus_tag="KPE55_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493156.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     ISMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2603..3664)
                     /locus_tag="KPE55_002093"
     CDS             complement(2603..3664)
                     /locus_tag="KPE55_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0493157.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVANFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(4275..5126)
                     /locus_tag="KPE55_002094"
     CDS             complement(4275..5126)
                     /locus_tag="KPE55_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0493158.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5143..6063)
                     /locus_tag="KPE55_002095"
     CDS             complement(5143..6063)
                     /locus_tag="KPE55_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG0493159.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6173..7087)
                     /gene="murQ"
                     /locus_tag="KPE55_002096"
     CDS             complement(6173..7087)
                     /gene="murQ"
                     /locus_tag="KPE55_002096"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG0493160.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7186..8283)
                     /locus_tag="KPE55_002097"
     CDS             complement(7186..8283)
                     /locus_tag="KPE55_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0493161.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(8516..9862)
                     /locus_tag="KPE55_002098"
     CDS             complement(8516..9862)
                     /locus_tag="KPE55_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0493162.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(9951..10259)
                     /locus_tag="KPE55_002099"
     CDS             complement(9951..10259)
                     /locus_tag="KPE55_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493163.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10521..10919)
                     /locus_tag="KPE55_002100"
     CDS             complement(10521..10919)
                     /locus_tag="KPE55_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493164.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(10921..12081)
                     /locus_tag="KPE55_002101"
     CDS             complement(10921..12081)
                     /locus_tag="KPE55_002101"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG0493165.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12512..13531)
                     /locus_tag="KPE55_002102"
     CDS             complement(12512..13531)
                     /locus_tag="KPE55_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493166.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(13525..14205)
                     /locus_tag="KPE55_002103"
     CDS             complement(13525..14205)
                     /locus_tag="KPE55_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493167.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(14357..15827)
                     /locus_tag="KPE55_002104"
                     /pseudo
     CDS             complement(14357..15827)
                     /locus_tag="KPE55_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT         5106 a         2770 c         1670 g         6281 t
ORIGIN      
        1 caattagatt agaatctaac gctagtaata aaaaattact ttcccaaaat agatataagc
       61 ctcattttat cgttcaaata atgaaaatat atttgatttc atgcaaaaag agtgcctaat
      121 gaactaaaaa gttcattttg agacactcat ttttttatat aaatttatat agtttttaat
      181 cgttcgcatt caaccactca gttaagactt ttatatattt ctcattttct tcaacgaatg
      241 gcatatgtct tgaatactca aataattccc acttactatt aggtattcta tcatacatag
      301 tctttgcaat atatggtgaa cataaatcta ttgctccact agttactaga caaggctctt
      361 ttatttcatg taatctatct gtaaattcat atccagaaag agttcctgta ggagaaaatt
      421 cattttgtcc ccatcctaca atatatgctt cagaacctga ttttttaggt cttctcaggc
      481 actctggaga atcttctgtc acttcacctg cacaatacat ttccataaat ctttctaatg
      541 catcattata ttctttgcta gagtaatctc cagtatttac tgcatcaagt aatgcttttt
      601 ggtcaacctc actcatatat gatattcttc ttttttgttc tttctcccaa agttttgcag
      661 aagaaagagt agatgaaagg atgtaagact taataccttt tggtttatat tcaatagcat
      721 accaaatagc ctgcatacct ccccaagatt gacctaacag atgaatttca tcaagtccta
      781 gatgttttct tagttcaata agctcctcta tccaagtatc tgcattaaat agctcaggat
      841 gcccttctac aaatgaattt ccacaaccaa tttggtcata cattattact tgtctaccac
      901 tttcagctat cttatccaat acctcaaaat agttgtgtgt agaacctgga cctccatgaa
      961 gtaatactaa tggcttcttc ccttccgtat tttctcctac tatacggtaa tacgttttaa
     1021 aacctttaaa aggcatatat ccttcagtaa ttttcatgtt tatctcctcc aatttgtaat
     1081 tataaacaat aatcaagttc attaacaata ctatttaaaa cctcttatac atatttgatt
     1141 atgtatttta atctttatac ctacttttat aaatagtata acataaaagt taaaaatctc
     1201 tatactaaac caattataaa attatattaa ctatataatt ttatttttta ataatagtat
     1261 atacatattt gattattttc atttgctatt taaaatcaat tttaagtttt cttcttataa
     1321 taaacttcct ctttgtggtc tcatgttagt ctctaaattt aaaattttac aaaaaatttt
     1381 cataaaataa aattatattg attgtatcta aacaaatagg gcaataaaat aattaagtat
     1441 agttacttac ctttcaagca atattagttt tatgttagaa ttttattata ttgatttaca
     1501 ggaggtttgc atttgaataa tattaattca cttaaagtaa taaaattatc tcttggatgt
     1561 tctcttgcta tatttattgc ttggctatta aaattagaat actcaatggt tgctggagtt
     1621 atagtactac ttacagtaaa ggacactaaa agagaaactc ttaaaggctc cattggaaag
     1681 atttatggtt ttcttttatg tactattttt tcatatttat gttttaatat tttaggatat
     1741 aatttgactt cattttcaat atttatattt ataattatac cactttgttt tttgttaaat
     1801 attcaagatg ttattgctat gtgtgtagtc atagcatccc attactacct tcaaggagaa
     1861 acttctatca aatggatttt aaatgaagct ggaatatttg cagtaggaac tggaataggt
     1921 gtcttaataa atatgtatat tccaacaaat atacataaaa tccatgaagt tcaaaaaaag
     1981 ttacaggaag aagtgagtat tgtacttata gacattgcag atattattgt aaatcctaaa
     2041 aaagaaaatg gatatagtag agatttatat acattaaatt cacttattga cagcagtatt
     2101 tctgaaactt acgataatat taataatact ttattaagtg atactagatt cttcttagag
     2161 catatggata ttataaaaag ccaacgggat atcttagaaa atttgtatag ttatgtatct
     2221 caactaaatt ctacaccacc acaagctcat attctatctg cttttataca taagataggt
     2281 tatactgaat ttgaagctga aacaggcaat cttcttcttg aagaattaaa gagactaatg
     2341 attagtatga aaaaccagcc tctacctgtt gacagaacag agtttgaaaa tagagctatt
     2401 ttattcctat gcttgactga gttaaaacag tttttagtta atcgtaaaca tgctcagatg
     2461 cttagagata ataattttta taaataaaaa ggggagaaat ctagcagttg acctgactac
     2521 aaaatatcag atataatatt aaatgcgccc ctagtaagag ggcgcaatgt ttataactta
     2581 ttctactgtc atgattaaat cattattcat atcgaacata cattatttga ccaagtccaa
     2641 tattagcttt ttcatcagtg atttctattt taatagtcac atcatttttt ttacagtact
     2701 tcgctaattc tgcaccagaa cgatttttac cactaaaatc taaagaaaat ggcttatcat
     2761 atttaaaagg atattcaaca ggtttaccct ctggtgctaa tgcaaacatc ccatatggtg
     2821 tgttcataaa tcctattaca gcaatcgtaa gatttgatgg gtctaaattt gattcactac
     2881 ctgtaaatgt aattgtattt actgatgaat taactacact acatctaaca ccttcataca
     2941 ttacatgttt ttcatttata tcttctcctt tttttccaaa atttgcaaca gcctttgccc
     3001 cttcttgatg tggtaacata gtgtgcttcc caggtggtgc tggtttctgt gtctgttctg
     3061 gttttaccgt ctgtactatt gcaccaactt ttggatatgc tgtcttatca aatggtaatg
     3121 ggtggctaag actcttgtat atgatatcct ttcctgctga tttattagca tcaatatcta
     3181 catgatacaa acgcccatca ccaaaacgga taagattcca ctggtggtca tttgatgtgc
     3241 tgtgtacata ataagattca attcccatag catcacacaa agctttcatg ctcctagcca
     3301 ttcctgagca tgcacctttt ttagtaacca atgtataggc agtatttgct ccctctactg
     3361 cattccagtc aacatatgtt gtgttatttt taatgtgatt gtaggcaact aaaatcttag
     3421 catctgtggt catattcttt gtgattttct tgttcataaa atctgctgtt gcttgtgcta
     3481 ctgagtttag ttgggcttgt gacagctttg gaccatatac gctattggtt ccagtgacat
     3541 agctttttgg tgtttttgct gttacctctt tggctgtcat accataaact gtcatactca
     3601 tacctgagat taaaactatt gttgtaatca ataacttggt agctaacttc ctaattttct
     3661 tcatacttaa tattctcctt tataaaacat tgttacttcc tattctctac cattaataaa
     3721 attaatttag gatttaaaaa ggtggattgt caacatgtat atttctccat tcatacattc
     3781 gtgaaatatt taaatttcat ggaaaatcta atagatattt tctagataat ttgtatacaa
     3841 tacaactcgc aaatcagggt gtttgctgtt agtttgagaa aatgatgatg attaatacat
     3901 tcggaatctt tgacctataa taaataccat tgtcttcaat cattaataat ggtaagagta
     3961 aagagaatta tcaataatac agttaaaatc aactttgtat acatttcata cattctctcc
     4021 caacaacatt atacttctta taatttgtat attcaatacg ttttctggga aaatatagat
     4081 atatctataa aaatataaaa aaggagttga acttaaaatt tgattaaaaa aaatcaattt
     4141 taagacaact catttgtatt ataaaaatgc tatatattat tattaagtaa gtcaatattt
     4201 tagatattta tctatttctt attgacttac gcttatagtc taacttattt gtttagttga
     4261 tatataacct agttttaagc attattaata tttaaataat caagtatcct ttttgtttct
     4321 tgtagtttct gttctgcctc atttaagttt tcttgaatta tagaaatata tattatatct
     4381 atcaacgtaa gcattgctat tcttgaagaa atagctcctt ctctaagtct tttttctata
     4441 tttggtacta ctagctttaa atcactaatc ttactaagat gattgtcact atatttagtt
     4501 atacaaatga ccttacatcc ttttaatttt gcattttcta tagactttat tacttctttt
     4561 gattctccag agtaagatat tgctatagct atatcttctt tatctacatt tgaagatacc
     4621 ataagttgtg tatgactatc taaagaggta aatgcctgtt tatttatcct taaaagtttc
     4681 atctgtaaat ctagtgcaac taaagccgaa ccacctactc caaatatata tacattttta
     4741 gcctttatta tctgttcaac tgcttctttt attatatctt tttcattcaa caaccttgtt
     4801 tggttgatag tctctatgtt tttgtttgta attttgttta taacactatc tatattacaa
     4861 tcatagtttt catctacata tgtgtattca ttttctttgc ccatcaaatc tttagctatc
     4921 tcaattttta aatccccaaa acccgaatat cccatttttc tagaaaacct aactatactt
     4981 gctgtacttg tttttgagtt tgacgccaat tctgatacag aaagcttact tatagcctct
     5041 gtattattaa gtatgtaatc agctattttt ttatctactt ccgttaaatc ttcatatatc
     5101 tgttttaatt ttaaaatgca actcatactc taccacctct acctatttac tataatattt
     5161 cttcttagca agtaaacatg ctcctaaatc tggagtcaac actggtctca ctataccaaa
     5221 tctcgtttta ttcaaatatt ttttaagtgg ctctaatatt aaatctcctg acttaaacac
     5281 tccacctgaa taagatacct ttatctttgt accttgttca aaatgatgct ctaatcctat
     5341 tataagccta cttaattcat aagctgcatc atcaaatatt ttactagcag tattatcacc
     5401 ttcaataact gcttttaaac atatcgtagc aaactttgct atttctattc tatctccttg
     5461 tatttcatca tttacatatt ttattatgtc ataatcattc tctatattaa gatgtctttt
     5521 tattaaatca tataattttg tttttgaaca ccttccatca gactgcattg tgaacatttt
     5581 taaggtagcc attcctatat aatacgctga accttcatct cctaatacat agccccatcc
     5641 tccacatctg tgaagattcc catctttatc taaagcctgt gctattgaac cagtacctgc
     5701 aattatattt ataccttttt ccccatttaa agaacctgct aatgctattt ctacatcatt
     5761 acctagagta taatttatat gactatatgc attttttgtt gctacttcta atgcatacaa
     5821 tacttctttt atttttccat atccagcaag tccaacaaat gcatacgtta tttcttctac
     5881 atttatctta gcatctttac aaacttcttc caacccagtt attaataatt tcgttagatt
     5941 atcaaatcct atttgattat aatgacaggt tcctttggtt atggttccta ctatatttaa
     6001 ttcttcatct actaatgtaa atgttgtttt cgttcctcca ccatctacac ctaaaaaata
     6061 catactatcc tcctacaata tctttaataa ttctttcaat acttaatatc taaaaataac
     6121 cttccaatgc tgttttttta atattgaaag gttatcagta ctattattta ttttacaatt
     6181 tttctattga ttttaaagct tcacttatat aaccttcttg tctatctagg atactctttg
     6241 cactatcctt atctaaacca ctttttatca taaatatagc caatttaacg ctattatctg
     6301 cttcttctaa gaatttttct gcaacatcat attttacatt tgtagctatc attattattc
     6361 tttttgctct ttcaaccaac ttttcatttg atgtcttaac atctaccatc aagtttgagt
     6421 atacttttcc catcttaacc atagaagctg ttgttaacat attacatacc attttttgag
     6481 ctgtaccact tttcattcta gttgagccca ttattacttc tgcccctact ataatgttta
     6541 ttggtaaatc cgctttttta ctcatttcac tattcggatt catagttatt gatagtactg
     6601 cacaccccaa ttccttagca tactccattg cacctataac ataaggagtc cttcctgatg
     6661 cagctatacc acatataaca tccttagatg taactcctct atctattata tcttgctttc
     6721 caagttcttt atcatcttca gcaccttcta ttgctttaaa tattgcttga tttccaccag
     6781 ctattatagc ttgaacaagc tcaaaatcca ctccgtaagt tggaggacat tctgtggcat
     6841 ctagtattcc tatcctacca ctggtacctg ctcctatata gaataatcta cctccctgtt
     6901 tcattctatc tactattata ttaacagctt ttgctatact ctccttttgt tcttcaacag
     6961 cataggctac agttttatct tcgttattta ttaattctat tatgcctaat gtatcttctt
     7021 tatctatttg caatgtattc ttatttctac cttctgtaac taaattttct aaagttttta
     7081 ttaacatatc atttcctcat tttcttaata ttaaattttt aaacacattt taattccaac
     7141 tttcataatt atatttttat aataaattaa tattttcata ataaattatt ttgttgaggg
     7201 tttaaaatta aacttttgcc atggtttaat ataatctaat ataaataatt cttcttttct
     7261 tatatatcct actacattcg atttaccact gtttttcata tcagtcaagg ctatttgtaa
     7321 ctcccctgca taatgaccat attcactact ttcaattact atatctcctc tttttattat
     7381 tttaggagta ttaaatagtt caaatttgta tcctttatat ttaacacgag attgagtaga
     7441 ccttatcaga ctgtcagata tgtcccctct attaaaatgt atttcttcaa agagtatctt
     7501 cttctcaact tctcgtatat tttcctctat tgctacatca aatgtaacca tatcttttct
     7561 catagaacct aataccttta gctcctcata tgttggataa caatttgata ttattatatc
     7621 atttatatta ttcatagcta ttatatgctt tacttgaata tctataggta gatttctgtg
     7681 catctcaatt gttggaagcc catcagttac aggccatggt ccaaatgtag tttcattttg
     7741 acttgttaca aaaactgctg ttcttaatcc atacttatta aatctttctg tacattttat
     7801 aaagtgttct aaatttaatc cactatatct atgtggataa aagttatgac atcctatcaa
     7861 attttcttta tttggttggt agtccattat tgtatctata taattagtat tattactcat
     7921 attgatttct attttaagat tttgaggatt aaaagtcatt aatgcctctt cagaacctgt
     7981 aaatcccata tcaagcctta ttccgtctgc cttcgtcttt ttaaaaaaat tcaaatcttt
     8041 gtagcttatt tctaattcac caaatactct gggatttaca tcaagtatta cctcaaaacc
     8101 tttaaactta gcgtattcat ttatactttt aaatttttta attatatttt ctttagtatc
     8161 tttaactgat aataaacatg aaaatatacg tgtaaatcca ttttcagcag ctttatctat
     8221 ataatcatat atttccttag atgttgtttt ttctggataa attgatattc ctaatcttcc
     8281 catctgtgtc atctccttat atcctatatg tgtttaaatt tatttcgaat tacacgaaac
     8341 ttttttaaaa atttgatgaa aaaaagtttc atatatattt ataaaaaaag aaaagtttac
     8401 cttttctttt tttataaaca atcaacaaaa tatcttcgtt acttatttca taactgttat
     8461 actttgaaat ttatactaat ctaatatagt ttttaatttc caatatagtt tctaattaat
     8521 aagcttcttg ctcttcaact ttttcttcat gaagcaacct cttatcgtac atcttgacaa
     8581 atggatagta aattattatt gatatagcta tacataataa gttaagaact gaagctctcc
     8641 aatctccacc agtggctaaa tatgccccta ctggtcctgg taatgtccaa ggagcagtaa
     8701 ctactactct ggatactaaa ttagcactca tagcaaacca tgaaactgtt gccattacta
     8761 ttggtgccac aacaaatggt attattaata ttgggtttaa tactattggt gctccaaata
     8821 ttattggttc atttatattg aatattgctg gtattacagt tgcttttcct aaagactttc
     8881 cataagctga ttttgctcta aatgcaagaa gtaaagctaa gcctattgta gctccagaac
     8941 ctccaatcca tataaaccat tgaaaaaagg gttcagcagc aatacctgga actgcttgac
     9001 ccgcagcata tgaagctgta ttagcatcta ataattgtat ccataaaggt cttgccaatg
     9061 aacctattat tgatacacca tgtattccaa atgaccaaaa aaacgttatt aaaaatatta
     9121 gtataattac tgatggtaat gtgtcagtgg ctgaaactaa tggagttact aatttagcta
     9181 tgaagccatg ccaatcaaac cctaggaaat aagtaacaat agccattaaa attattatta
     9241 atgcggctgg tgtcagagct tcaaaagacc ttgtgactga tgctggaact gcttctggca
     9301 tggttatctt gaatttagat ttgtcagtaa atctataaat ctcaacacct attatagatg
     9361 ttacaatagc aacaaacatt gaacctccac caagattagc cattggcatt tgccatcctt
     9421 gaggaattga attataccaa gctaaaagtt ctggactctt ttcaactatc tttaataaat
     9481 caggggtaag accctctatt gcagtaggaa ctattgtcaa aaggaatgta gctgtagcta
     9541 ataaacctcc tgtcaatcca tctaacttat atgactttgc caaactataa cctatgccaa
     9601 atgtagaata tactgatata ataaaaaatg tcattctata tggtagtaat attgtaactg
     9661 cattttcctt tagatactga gttacccccc atgaatctgg taatggagga aatgctaata
     9721 tcaagaaaaa tgacccaact attataagtg gtaatgaagc tacgattcca tctcttacag
     9781 ctcttaagtg acgttgttct gccaatctgg ccatagaagt tgataattta gtgtctaaaa
     9841 attctgtaaa tttatctagc atcttttctc tccttaatta atatttattt ttattatcat
     9901 taaaattttt ataaaatcaa ctttcttcta atctaacaac actgcaacta ctatttactc
     9961 aacagttctt gaacttgttt taataacttt ggtcctccta atggactata agcttgtgct
    10021 tgtattaatg cacatggtac actaacctca tcagcaaaag cttttaaata gtcaaatcta
    10081 tgtctgactt gtggagctac catagcaata tcccatccat ttttaacttc attctcaaac
    10141 tcttgagtac caacagccaa aacatctata tctattccat tttttgctcc ttctttttgt
    10201 aatgcattaa ctgctatagc acttgacatc cctgatgaac aaacaaataa tactctcata
    10261 ttgattcccc ctgttttttt atttcttttc aatcattata gcatgtattt ttgattacag
    10321 gaatacgttt gtatatattt tttcatattt tacattttaa attgtaattt tatttcatgt
    10381 atttatatct tgctttaatt gctttattta tctaatcatt cttttgtcta aaattacaca
    10441 ctacttaaaa tactcatatc ttttcttaaa aaagaaaaaa gactatctta ttattaacat
    10501 aaaatagtct caatactttt ttaaatatgt attcctttat atatccagaa gtaacctatt
    10561 attgcaaatg ataaagcaaa taaaatatac agtctaaaaa atcccaaaga aaaacctctc
    10621 ttagtttcaa caaagtatct tgtatatgca actgcaaaag atgtaattaa tgaatttcct
    10681 ataacaactc caaatctatt atctattcca aatatagata ataacaaagg aaaaactaca
    10741 gtacttacta gagatgaaac tattaatatt aatccacttt taaaacttat agttggaata
    10801 ttataatctg ttctttgatt tttctccatt tgtttcttag tatttttatt actttttgta
    10861 ctttttgcat ttttataatt attatttttc tttcttttct tttttttatt attagacatg
    10921 tcatttacct ccaaaaggtt taggtgttat attccctaaa ataactttat ttttagcccc
    10981 tgttgcagat attacattag aaaaagaatt atttaatgtt tcattgccca agattacaaa
    11041 tgccaaagcc tcctttgcat cagaagaata cccatattca tcttgagtta atacttcaac
    11101 ctctaataat aactctttta tatatcctat taaagttaga ttatgagctc ctccacctcc
    11161 aattaataaa gtatctacat taacctttgg aagtatgaag tttttataat tataatatat
    11221 agcttttgcc gtaaacatag ttactgttgc tatcaaatca tatttatcta catgtttata
    11281 tttgttaagc atgttatcca aataaacttg tccaaatacc tctcttccag tgcttttagg
    11341 tggcgcttga ctaagatact tatgactcat caaatctttt aacatttctt cattaatttt
    11401 accttttgat gcaaagtatc catttttatc atactttcta ttaaataatc tttgacatac
    11461 tccatctatt atcatatttc ctggtcctgt atcaaatgca aatacatcat ctatgttaca
    11521 tgatttaggt attatagtaa tatttcctat tccaccaata ttttgaagtg ctaaattttt
    11581 attgttatca ctatataata aaaattcact atatggtact aatggagcac cttctccacc
    11641 tgctgctata tccattactc taaaattaga tattaccttt gtatttgttt catatgctat
    11701 aactgctggt tcacctattt gaagcgttga agatacaaaa ttattataac ttcttggtat
    11761 atgaaagatt gtttgaccat gagatgcgat aaaatccaaa tttgctatat gaaagtttgc
    11821 tttatgacac actgatttta cagctttact aaataaatat ccaagcttaa aatttaagct
    11881 acagattaaa tcaacactcg attcctcttc tatacagcac tttcttattt cgtcttttat
    11941 atctttagga atttctagag ttataaattc tatttgctta actttagtat ttctaccatt
    12001 cccactaatt tcagctagta ctgcatcaac tccatctaag gaagttcctg acattaaacc
    12061 tacactatac atagttatca tccttttaag tttatagtta ttcctcccca aatcaaggta
    12121 ttaagtttaa taattttaga attaatctta tttcatcttt aattacgata atttactctt
    12181 atgaaattaa ttgagttaat cttatacacg ttttttacct tagctaaatc ttacattaat
    12241 ttattttcag tgtcaatgta atactatgta attttttttc acaaacaaaa catattctct
    12301 ctccaaaaac catattaaaa tagttactac aaataaaaaa cataccaggt acatatctga
    12361 tatgtttttc atatataata attattattt tataatatag atagaacatc atttaaggca
    12421 ctaattaaat cttcactatt ttcaagaact aaatttattc ttattgtata tgtcgatttg
    12481 tatatgatga tagattttat agtctaataa cctaactatc taggcttaaa taatctgata
    12541 tatcataaaa atagatatta aaaattgtat atgaaccatt ttccgaacat acctcaatct
    12601 catgccctaa actatctatc attttcttta caaaatacat ccccattcca gtagacttat
    12661 acaattgatt tcttccattt ttccccgtaa atcccttgtc aaaaacccta gatatatctt
    12721 cctcatttat acctatacca aaatctttta tagataatac cactccattt tctaactttt
    12781 ttgaattaaa ttcaatctta ccaacttcct ttgaatactt tatagcatta ttaataattt
    12841 ggtctaaaac atatgacatg catttagaat ctgtgtaaac atcattttct aattcattca
    12901 aactaacttc tacattattt tttataagca aaaatgaatt attttttata gacattttta
    12961 ccaatttttc taaattaaat ttatttataa atacatcttc aaacatagtg ttgcttctac
    13021 tactatacaa aatagagttt actaaaaaat ttatctttgc aacctgattc ttaatatcca
    13081 aactatcttc tatgctactt aacctatctg taattatact taaagaagat attggaattt
    13141 ttatttcatg aacccattta gctatgtact catccaattc ttttaatgat tgtttataag
    13201 tctctatgtc actattattt tttattttta agttttctat aatatctttt attagatttt
    13261 cttcaaaaga ttgtccatca actaaataac tgtctatttc accactattt tctatacaat
    13321 tatataaatt tgtgtaacta tttctaaaat ttatataatc tataacaaaa aataccatta
    13381 ttataaacag cactaaaaaa tctaaatata ttaactctga atatttattt ttaaaaatat
    13441 ctatagcaaa taagtagata tttattatac taactacaac taaaggtaga gcaatatact
    13501 tagacctacc tttcaaatat tttactagca ttgtattata tatcctatcc ccttcttagt
    13561 ttttataaaa tcatcaaaac ctagttcttt aactttactt ctcaatctat tcatatttac
    13621 agtaagtgca ttgtctgtca caaactcatc attatcccat agagtcatca taagactttc
    13681 ccttgataca accctattct tattttttaa taataatgtt aaaattttta tttcgttttt
    13741 agtcaactct atacttttat ccttatactt atgttttata atccctttcc ccacatcaaa
    13801 catcaaatct tcacaataca aaatactatt agaatcacta taattataga ctcttcttat
    13861 tataccattt attttggtaa ccaatatttc tatagaaaat ggtttggtaa tgtaatcatc
    13921 tgctccatta tttataccca ttattaaatc catatcagaa tctcttgatg ataaaaatat
    13981 taatggtaca tttgaaatag ctcttatttt ttcgcaccag taaaatccat tatataatgg
    14041 taaatttaca tctaaaagta ctacatctgg ttttttatct ataaattcat ctaatactac
    14101 ctcaaaatca tcaattaatt ctactttaaa tccccatttt tcaagtgcta atgcaatctc
    14161 ttttgataaa tcaatatcgt cctctactaa taaaattcta tacatttgta tattctccat
    14221 tatcctaata tttcactatg ctatgaatta ttaatatcat aacatatttt atatgaattt
    14281 attacccaaa ttatttagtt aatcaacaag gagactctta ttacaagaat ctcctttctc
    14341 tttattaata tatcaactaa tttttataca ctacactctt aaacttatta acagatactg
    14401 ctccatatat tatgaatatt atcaaacata tcactaaact tagtatatat ataccaaata
    14461 aatcggtcat caataaatct cctgctaatc tcaatgcaaa ataactagat atagttgcac
    14521 ttaacattgg caaagtataa aaaatagcta cttctttaag tatagctcta tttatatctt
    14581 tttctgtagt acctattttt aacaaaatct tatatttctc tttatcttca tatgcatcac
    14641 tatatatttt catatatatt atacttccag ctacaattgc aaagactaag aataaaaaca
    14701 cactacatat gcaaacttca tccattgact ctcaccttga gctttatatc cattttgaat
    14761 atacacattt tttaaactta aattcttttc aatattgttg aacattactt gactattttc
    14821 ttcttgaaat acctttgcac cataaaaata cacttgttga cccttatctc ttagtttaaa
    14881 atatgtatca tcatttacca ctataatact ttgattaagt cctgtcccta gtactggaac
    14941 ttttacttct ccccttgaaa tattatataa ctgaccatca agttttatct tggcttcttt
    15001 tgtagctatt gatgctatcg tttgagggct ttttaactct attacattat ttccactaac
    15061 taatctttca ccatactctt ttaattcttt atcattgcca ttcactttaa gagtattaca
    15121 aaaatcacta tatttcatta tagtatctgt tgtatcataa gcttctttta tatctgtttt
    15181 ctttaaaatt gttagttctg gatttactga gtactttatc ttattagtat cttttattat
    15241 attctctatt tgttttttat tcacttcatc tttatccatt gaaactatgc taatagtgta
    15301 cacatttctt tgttcttggg cattcttatg tatacgcttc atcgcaactg aagcaccaag
    15361 agttgatatt gttgtagtta taattatagc tattatagca taagttttat agtttttatt
    15421 taatcggtag tttatattgt ttattgcaat tatattattt ccattaaata atattttttt
    15481 atttttgatt ataatatcaa atataacagg cataactgat ttgaataatc cataagtacc
    15541 tactattatt atacctataa caggtagtac actagttata tctccagttc ttatattggt
    15601 agaaagatag tagccatatc ctattactat aattgatact atagctaata tatatatacc
    15661 tattcctacc tttggtattt tttcttgttt tttagatata ttgattagat ttattacact
    15721 acttctacat attgttataa aaccttttac agccattatt ataaatatag aaataaagat
    15781 taaagtagca tatagtatag cttctatact aagtccatta gatacct
//
LOCUS       DAELNY010000078        15584 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.078, whole genome shotgun sequence.
ACCESSION   DAELNY010000078 DAELNY010000000
VERSION     DAELNY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.078"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(29..370)
                     /locus_tag="KPE55_002105"
     CDS             complement(29..370)
                     /locus_tag="KPE55_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493168.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(397..1068)
                     /locus_tag="KPE55_002106"
     CDS             complement(397..1068)
                     /locus_tag="KPE55_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493169.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(1080..3656)
                     /locus_tag="KPE55_002107"
     CDS             complement(1080..3656)
                     /locus_tag="KPE55_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0493170.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            3864..4799
                     /locus_tag="KPE55_002108"
     CDS             3864..4799
                     /locus_tag="KPE55_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0493171.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            4818..5555
                     /locus_tag="KPE55_002109"
     CDS             4818..5555
                     /locus_tag="KPE55_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493172.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(5740..10797)
                     /locus_tag="KPE55_002110"
     CDS             complement(5740..10797)
                     /locus_tag="KPE55_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG0493173.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(10822..11241)
                     /locus_tag="KPE55_002111"
     CDS             complement(10822..11241)
                     /locus_tag="KPE55_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493174.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(11475..11726)
                     /locus_tag="KPE55_002112"
     CDS             complement(11475..11726)
                     /locus_tag="KPE55_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493175.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            11815..12546
                     /locus_tag="KPE55_002113"
     CDS             11815..12546
                     /locus_tag="KPE55_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG0493176.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            12605..13516
                     /locus_tag="KPE55_002114"
     CDS             12605..13516
                     /locus_tag="KPE55_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493177.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            13567..14379
                     /locus_tag="KPE55_002115"
     CDS             13567..14379
                     /locus_tag="KPE55_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG0493178.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            14488..15180
                     /locus_tag="KPE55_002116"
     CDS             14488..15180
                     /locus_tag="KPE55_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0493179.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            15267..>15584
                     /locus_tag="KPE55_002117"
     CDS             15267..>15584
                     /locus_tag="KPE55_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493180.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQ"
BASE COUNT         5545 a         2060 c         2165 g         5814 t
ORIGIN      
        1 aaaattgggg cctattaagg cccccttact attttttgat agatttattt aaaattactt
       61 tctcattttg attttttata tttattatag agtcagtata atccacttca aagttggcaa
      121 aagcagagaa taaatctata tctcttaaca aatgaattgt catacattga ttgttcttat
      181 ttagccttag aatcaatgct cttacaaatg gaaattgtat tctagaatta aataagttag
      241 aaccatcagt atctatatct aagctacgat aattaatatc tataaatata tctacatctt
      301 cattttcttc tcttattatt ctgtagtcaa aatccaagtt taaatttaaa ttacctaaaa
      361 tcaataacat ttaaatcctc ttttctataa atagttttag agtgttaatt taagctctcc
      421 atctattata gatataattc tatctgcttt tctagcaata ttttcattat gtgttattat
      481 tataagtgtt tggttatatt tttttataga actttttagt aaatccatga cttcttctgt
      541 agttttactg tccaaatttc ctgttggttc atctgctagg attatacttg gttttgcaga
      601 taaagcacgt gcaatagcaa ctctttgctg ttgaccacca gataattggt tgggaagatg
      661 atttttcctt tcattaagac ccaataaatt tataaggtca tcaatatatt ctttatctat
      721 ttttttattg tctaacttta gtggtaattc tatattttcc tttacattta atacaggaat
      781 taagttatac tgttgaaaga caaatcctac cttttgtctt ctatatcttg ctaattcttt
      841 atctgaaaga caacatatat ctttaccatc tacctttata gtaccattac ttggtctttc
      901 aagaccacct actaaatgta gaaatgtact tttcccactt ccacttggtc caattatagc
      961 aacaaattcg cctttattta tttttaaatt tgcattcttt agtgcatgta cagatgtttc
     1021 cttttttcca taaatcttac ctaagttaat agtttccaat ataggcataa ttattcctcc
     1081 tattctgtaa tgtttatagc ttcaactata ctaattttat ttatctttct tgatggaata
     1141 tatgtagcta gaatacctac aattatattt gctacaacaa ctagtatata tattttgtaa
     1201 tctattgaaa atcccaatcc atatgataca aagccctgca tataatacat tttaaattgt
     1261 attataattc ctgacacgat agtaattata cttgagagta caccatataa tattccttca
     1321 taaagtatca tatttttaaa gcctctctca gagataccaa tagctcttaa cattccaaat
     1381 tcactagttc ttgaagttaa gttataactt atgttgttta ttatgttaag tacactgatt
     1441 atgaacatta ttgctactat accataacta taaatcatag ttctagatgt catcttatca
     1501 gaattttctt tttctaccat catatctact gttgtagttc caggaacttc acttccaatt
     1561 ttacctaaaa ggttatttat ttttttcttg tttgcatttt cattaaggtc tgcataaact
     1621 aagttatact tatctacacc agttaatttt tttaggtatt cgctactcgt tattacatct
     1681 acaccatcat ctccagagta cataccatta tctgcaaaag gataacttat aatagctcca
     1741 actttaaatt catattctaa gtattcataa ttatctttta gactttcata aagacctgta
     1801 tccatcccac cctttggata tttgactttt atagtatcac ctacttttat atctacagct
     1861 ggtgttcctt caccaggagt aaaagtcttt gtgtatttct gtgttttaga aatttctggt
     1921 acatatacaa tagctaaatt ttcttttttc atttttttca agtttatatt tccacttact
     1981 aagtattttt ctaatgactt tatcatctca tcattataac cttttagctt ttgctttagt
     2041 gtatatttgc cagtagtctt attttttatg aacagaggat ttagcttata ataaggagtt
     2101 gaatttatag cttcgtagta atctaatcta tttattttat ttacctctat tcttgtgttt
     2161 aagatactag cagttttgac tttgtcaatt ccatctatat tttttatttt atctatctga
     2221 ttatcattaa acccttttat atctttaaaa gaagtattgt taccaatttc attgagataa
     2281 acatttgata gaataaaatc tgcattcata tatgtttgtt cttgagtaga tttgttttgc
     2341 acatctaaaa aagaatattt ataattgtct tttatgacga ttatacctcc tatactcata
     2401 gataatacaa ttataatgaa ccccttttta tttctaaaga tgtttttcgc agatatagat
     2461 tttgttactg acatatttaa agttaatttt gatattaaat aattaacttt ggttacattt
     2521 ttatcagctc caaaagtttt tctgatagca tcaataggag aaattcttct tatttttaag
     2581 tatgtaaaga aacttataat tagtattatt agtattgtac atgcaattga taagagaatt
     2641 attttatctg gaataacaaa aggtgtaaca tttatatttc cttcaaattg aatatttcct
     2701 acagacctat taaatatttg tgcacctccc atacctatac atatacctat aggcattgct
     2761 attagagcaa gtatgaaaag ttcataaagc ataagtttga aaatcctaaa gttggtggaa
     2821 ccaatagctc ttaagacgcc atattcctgt actctttggt atattgatat aacatagata
     2881 ctatatatta caataccagc aaatagactc atagcaattg caacgtattt acttgaatta
     2941 tctaaagtac cgtttttcat agtagattct ataagcattg agtttcttcc tatacttttc
     3001 tcatctaaca tagcattttt tattatattg tctatgtttg tatttatatc actgccttca
     3061 ttaaattcaa tataagttgt aaaatcattc agttctgatt cattgagatt agaaaaaatt
     3121 tcacattgtc cctttttttt ctctataaat ctatcttcaa ggattccaac aactttaaaa
     3181 gtttctggat tttctttttg atataattta aaagtaaggt tttgatttat ctctggtttt
     3241 aagcccatac tatttaaaat ccatttctcg actaccacct cattgctagc ttttggaaat
     3301 cgacctttta taagtttaga atgactagtc aaataatttt tatttgtata tagtatgttt
     3361 ataggtaatt tttcattaac atctgtagac gcatagtaag atgttatacc cagattttta
     3421 atatctttgc tagattttat ggactttaat tgatttttct ttatattttt aaagtataca
     3481 tggtcagttc caagctctct tttcaatcta tctacatctg cttgctgagc acttcttgag
     3541 agagtaccaa taccaacaac gagtgatgta gcaagaatta tactcacaac aattgctaaa
     3601 gacctagctt tataactttt tatatacctt agtactattt tcaaattatc tttcatattt
     3661 accacttcct ttcttaacta aatcaattat atatttatct aaaatatata gcaaagtaag
     3721 tattttcaaa ttttaagtag ttaattgtac aaattcatct gtatttttac aagtttaatt
     3781 aatatatatg taaattttaa ctttttacat aattttttta aaatgatata atccctatat
     3841 aaattgtaga ggggagggga atcatggata acaattttgt atttcctata gtaatactaa
     3901 ctttgtttat atttagcaat cttataatga ataagtataa attttacaaa gaattagaca
     3961 tattaaaaag aggcatatta acatttttta tatcgtgttt tgcattgatt ttatttatgg
     4021 agggcactaa atatatacaa ttaaggttag gatatagttt tttacttgaa ggagtattaa
     4081 atattatact ttttttggtt ttaattattt taagttttta tacaggcttt aaagttataa
     4141 gattgataaa taataataat gaacaggaat ataaattaca aaaactagat agtaaaataa
     4201 atcataagga taatataatt atcaaattaa gaagtcaaaa acatgactat ttgaaacatt
     4261 tgcaagtagt gtattctcta ttaaatactg ggctagatga agatgccaaa gaatatataa
     4321 tgaatatatc aaatagcttt aaatcaaaaa attcaaggta tggaaaagta tcttatttag
     4381 atgctatagc ttcattaaaa tacgaggaat gtcttgaaaa ggatatatct tttgatgtct
     4441 acattcaaga ttttatggat aatttagaga ttgaacctaa tgatatgagt tcagtactat
     4501 taaacataat agataatgca atagagtctt tgtctactat aaaaaaagaa aagaagtata
     4561 taagattaca tgcatatgaa gatgaatttc agtatattat agctataaaa aataatggtc
     4621 ctaaaataca taatgttgaa agtatatttt ggaatggatt tagtactaaa gaaggacaag
     4681 ataggggata tgggctacat ttagttaaaa atatattaga taagtatgat tatagtatac
     4741 ttgtaaatag tgatgatttt ttaactgaat ttgtaatctt aatcccaaaa tattattagt
     4801 taggaggaga aaagtctatg ataaaagttt tgatttgtga tgatgataag attgtaagag
     4861 atgatatagc aaatatagct aaagagtctg gatatgtaga ttatgttaaa aaagttaagg
     4921 atggtataga ggcaattgaa tttataaaaa atgagaaaat agatttattg attattgaca
     4981 ttgatatgcc atataaaaat gggattgatt ctgctgaaga aattctttct atagataagg
     5041 atatacatat agtatttgtg acgggtttcg ctgattatag tcttgaaagc tttaaagtgc
     5101 atcctataga ttttgtagtt aaaccattta aaaaagaaaa aatacttgat tcaattaaca
     5161 tagcaataga ccatattaac tcccataaga ttgctaataa taattttata gaagacaccc
     5221 tttttgtata taaaataagg aagcagatac atatgattaa ttttgatgat atagttatgt
     5281 ttgagaaaaa ttcaagagca attaatctac acactaaaga tgaagatata ataaagtttt
     5341 atgagaactt tgatgattta aaaaaacgcc tacctcctaa tttctttatg acccatagac
     5401 aatacatagt aaatctaaga tttatacata aggttatacc tataaataaa tcttcattag
     5461 agataaggtt tataaatttt caagaaaatg ccatattaaa caaatctcta gaaaaagatt
     5521 ttttatatag attttatagg gttaaaagat tctagtgaag atagaagaaa aatacttttt
     5581 gtgtgatata ataagtaccg taaagataat gtataagaga agtttatata aaaattattt
     5641 gtaattatga tttaatctat ataaattaca gtatagtata tattggactt tctaattttt
     5701 ttaagaaagt ccaatatatt ttagagatag tttttatact tataaattac ttaagatact
     5761 agggtcttta atcttactat cttcagcaag taatagtatc ttagacataa tctcagcagt
     5821 tttagggtct tcatcagcga atggaaggaa tatcttacct ctactttgag aatgtatagg
     5881 aagtatattc aaagcaccca tccctttttt atgtacaata ccagaaccca tatgcacaga
     5941 atactcacct aaattacctg ctataagagc atgagagcct tcaaaattga cattgttaat
     6001 ttttaataat aataaaagtt ctttagcaat tgctatacgc atctctacag tagaatggct
     6061 agtttcaggg tcaacaccac cagcatgagc gacacttaca acaaggtcta tatcacgcat
     6121 aacttctgag aatagaattg gaggtataga agctaattct aaacttttat aagtttttct
     6181 atcaaaaaat tctactgttt caatagtagg aggctctata tctgctggtg aaaaccaatc
     6241 tgcaagtgca aacatctttg ccactatatt ttctttataa aatactttct gtaatccctc
     6301 ttcataatcg acagtccaaa gctgatttct aagaagtgct acagttttct ttggctgaat
     6361 ttggtgacca gcatatctgc gagatatagt tttttcagat aattcatcac tgtttattct
     6421 ataatactca cggaatactt gcttaaatgg ttgaacaatt tgcttactaa ataaatcatg
     6481 ttggaactca ctccacttac cagattcaaa caaatgaact ggatgtgcaa gttttaattt
     6541 attttttgcc gtcaacttat aagtttcacc ttctatattg caaataaaga acttagactt
     6601 atcattataa acatatccaa tattatcatc acatacccat actagatttt taatcaaagg
     6661 gcagataact ggatttttat agatattggc aatttcttta actgtaaata ctatttcatt
     6721 tttcatagct tgttctaaac tctgttttgc acgagaatac tgactcttta aatctttttg
     6781 gacagattta agttcaagta catatggatg tttagcaata gcttttggta tactctttaa
     6841 agacttgttg cctttttgaa ctttagtaga tgcttttccg ttttcatcaa catctatcca
     6901 aattgaagta ccatcaatat cttttggttt aaatagatga gcaacttcat ttagtttttc
     6961 agtctcaaga gcccacatca tacgatttac atctccaaat ccagcacttc tagctagatt
     7021 ttctagcgct atagtacaag cttttgattc actttcacga cgttgtgcac caaattgctt
     7081 gctttcttta gcaaactttt gaataaattc ataacggaaa gttgcttctt ttaggtctcc
     7141 ttttttcata ggaagtaaag agaaacttct caatttatct tgatttctat ttgtttcaat
     7201 ttctttcatc aacatgtctt tatcaagctt accaagtgta gcatcagcat agagttgtgc
     7261 acgtctatga gagttaccac cactagttat atatttagct gacttgtaaa gtatattgaa
     7321 tctttcttcc ccaatagttt tatatgaatc taaaaaccaa tctacatcaa atgcaccatc
     7381 aatgaaacta cgtggtgaaa taggggagaa tagagcaacc tctgattctt tttgtgcaga
     7441 gaagccttca tttatatgtg catggaagta ccaaattgta cttttcagac ccttccagtt
     7501 taaatatttt tcaattatgt ttacccattg aggggcatac atagcagatt ctatcaatct
     7561 taactcagta atgtcggtat ttttaagtaa actttttaat ttttttgaat catcttcttt
     7621 agtaggatag caatttttaa gtagagaact taaaacatct ttttttgaat aattgctacc
     7681 atatgaatat cctctctgga aagtttcttt accaagagcg accaaaatat ttacaaaata
     7741 atttatacct tcaaagtatt gtatattatt agctagatta gacacttctg ttggcatatc
     7801 acctctacgt gattcaattt ctataattcg gtttgtaaca gtttttaaat tatctttaag
     7861 ccaagggtac ttttctaatg tgtcgccagc ccatctagga tttgtcaatg ttcttattat
     7921 atttgatgcg ttgtatccac ttatcatttc tctattaact tgctcttcag tcattaatcc
     7981 taattcatgt gcacgagcaa acacatttat gctagtatag aaaggaaact tatattctag
     8041 caactgataa tttgaaaacg tagtgtagaa aaacttagta aaagaaatat catcataaat
     8101 aaacgaatgt gctttatcaa gccaatagtt taaaataggg gcatttaaag cagggcagta
     8161 ttcattttgt ctgtaatagt ttttagtaaa ttcaactctt tgttttttac attgttctat
     8221 agtcaataag tttctaagtt tatagtatat ttctaaacaa tagttaaata tttctttatg
     8281 gtcaaactca tttagtaaaa ctcttaaaag ttttcttgta agtccattaa atttaagttt
     8341 ttcaagtttt gtatatattt tattttgttt tttagagtca aggacatttg aatatataga
     8401 tgaaaaccaa cttttttctg tttcattata tccacatgta cttatactat aaaataagat
     8461 atcaagtatt aatttgtaat ctaagtcaag ttttttacta gtttctctcc aaagctctgc
     8521 taatggataa ttatcaagag tatcattaga aactttatat ttaataggag tcaagttata
     8581 agtagaataa cctaaaagta ctttgtctac attacctgac catctttcaa cttcatattc
     8641 ataatctgca ttttcagtaa taagttcatc aaatttttca taaactttta ttattttatt
     8701 tatatccgta tcgagaattt tttttgcttc tttttttgtt gctacagaaa cagaatcctc
     8761 acgagtagat aaacttatat ttgagataat tttattaagc tttgatgagc tattagaatc
     8821 atctttaaaa tcaaattgtt ctggaatttg aactttgttt tcagggttat atattccaaa
     8881 accattttct ttgcagtaat cattttttgc tttacctaac aaattatcaa ttagtatttg
     8941 aacttgagat gtattctttt tagatttatc aatatcctct ataagtaaaa taagcttatt
     9001 atatagttct tgtctagcag tattgtcttt taataagttt acaatttcaa gacctccaag
     9061 gcatttttga gaatttgaag attttatcag tgaagaagca atattttcca aatttgaatc
     9121 atcttgttca agtaaaattt ttattactgt ttgtctaata gagcttgtct ttaaagacaa
     9181 tagattggtt atcttatcta attctacatc tgttagctca agctttctaa gtagactcaa
     9241 taccgtttct ctattggaaa cactcttatc acttaaacat tccaataaaa attcacgttg
     9301 tctatctgtc tttggttcat ttacaaaatt gactataagt gaagctctta aatctgaact
     9361 cataagatga caattttcca taagcacatc tataaattga gcattcatat cgtatgctgt
     9421 taaagtcatc atacatttaa gtatttcatg agaagaaatt tcaatccaag tccatggaaa
     9481 tacacttcct gaaaatgatt ttgtctttga atttaaaatt tctaaagtat gttttaaact
     9541 ttcaaattgt tcttttctag catcaaagtc tttgtcaata ggagtgtttg acaagtcaaa
     9601 attagtttct ctgttttgcc aactatagta gtagccaaca gaaccttcta catagaaatt
     9661 tgtaattacc catgctagta tctctaaatc atcaccctta atgtttttag atgctataga
     9721 atgtttaaaa tatgtacttt ctgtatgcga taaaaagtaa agagctacaa gttttttata
     9781 tttttcctga caagaaagta agttttcaat aagagagtaa gagtcatata tgttgtaagt
     9841 tgcagtagcc cataaggcaa aataaatttc aagattatca ttgctattaa ttgattctat
     9901 acggaacttt tcatcagtca atgatttata tgaaagttct aaacatttac gtattattcc
     9961 aggtttttgg tctgaaaaac caagtcctgt ccatgtatcc aaggctctta taacagaact
    10021 aaatcgctct aaattgttgt ccaaaatagt ttttaacatg tatattgatg cagaaattgt
    10081 accctcatct aaactctcta caatttgctg tctaagtcct tcttgttgtt tggctgccaa
    10141 aagaagttta gaaagcattt catagcattt atcactatga ctcttcatga taccaattat
    10201 catttgtctt gtaagtagag cagtttgatt ttctccaaat attacttctt ctaaagcact
    10261 tattactttt tgatttcctt tgtctatttc tagtgctatt ttatcactca ttatagagtc
    10321 atagtacaag accttatttg gaagagtaag ataatctata actgaaaaat cataataaat
    10381 taaatcaaca tagtttgaaa taaaccttat aatacctcta aaataatcca caagtttatt
    10441 tgagcgatac cctttacgat aatatcctcc tgaatatgta taatccatct ttcttttaat
    10501 taattggcaa agttcttcta cttgttcaga agtaaatatt aattctaatg cattttttaa
    10561 aggtccagaa aggagctcgt ccatattttt aatttcgtag tagtggcaat tgtagtattt
    10621 ctttatcata tttaagaatt tgtcttcagg ttctctataa tagtatgaac tattttctat
    10681 gcagtcccaa agagactttg ctaagtcgta gtactgtttt gaagaagatt tagtaaaatt
    10741 atttttaaaa ttttcaatta ttttaggatt tttttctact ctagtacgtt ctaacattta
    10801 tgtacctcca aattatttat tttaaaacat tcttccagca agcaaggtaa gtcttataaa
    10861 agtaaatata tctccacttt tcagatttat agaggaatcg agtttttcaa taacatcctc
    10921 atttataagc actttgtaga ttccatcttc aaatgcctgt aacgctacct ctattgcctt
    10981 ttctacagat tgtttattct cattgtaaat agagccaaat ccaacttttc caatatcagc
    11041 ttgagattca atttcttcat tagttaagaa gtttattatt gatttttctg ggattttctc
    11101 attatattca agaacatttt tagatacaat ttcagagata gcttctttta ggcaattgat
    11161 gttgtcagat acatcaaaag gggtaagttc tagcataggt ttgcgtttgc caacagcctt
    11221 aactctaaaa taaatttgca taaaatcacc tcgcaaaata ttaattatgt atttagaaat
    11281 ttaaaaattt atctatttgt tatattatat cattttgtga attattaatg tgaattatgg
    11341 aattatttta caaaaaatag aaaaaaagct atcttaaagt ctaaaaaagc aataaaagat
    11401 aacttttaac tataattata tagaattata actgtacatt tattaaattt taattttaga
    11461 attatttttg ataactatct ttttttattt ataaattttg ctcctaaata ttttttgata
    11521 tcttcttcag gagaaaagtc atcaataagt atttctttcc cagtgtcttc aaatactaca
    11581 agaacttcac agtcaccttg taagaaaaat tttctaactg tatcttcttt aacttctcta
    11641 acttttatta cattcttttt ttctttttca aagtcttttg tgtaataact tcccatcata
    11701 ttaattattt ttgtatatct attcataaaa tacctccata tgcgattaaa tttaaaaaat
    11761 atgttataat aattgtttgt attaacttaa taattataac aaggagtaat aataatgact
    11821 agtataaaaa ataaagatat tttaaattgt atagattatt ccattaaaaa taagctcttt
    11881 gacaaactaa atgatgtata taagtccttg ccagtaggaa attgttctgg atgtggaaat
    11941 tgttgtatgg agtcagtagg tataaattta attgagttct tgaatatatt ttgttatctt
    12001 gaagatagag ttgatttaaa aaaaagctgt ataagtaaaa tattagatta ttattttgag
    12061 gagtatacaa gaaagaaccc atgtccattt aaagatacta ataatagatg tttaatatat
    12121 gaagttagac cattaaattg taggatgttt ggacattgga aaaaagagga ttataataaa
    12181 aatttagata atgttattga gaaaaataat aactatagag agttaatgaa aaagcaatat
    12241 ggatttgaaa taaatgatga agtagtaaat tacaggatag attattgtga tagatttatt
    12301 ccaaataaag attatttaag taaatctaag agattaagct tttttgatga gcttatgata
    12361 ttagactcaa atatttattc aagtggaaat atagatatag actttaggga tagaggtata
    12421 gttgaatatt ttatagagtc attactttat agaaatttag catataatgt gaaaataagg
    12481 atatctaaag aatacaaaat aagaaaaaga actatagata gaataaaaag gattgttctt
    12541 gtatagtttt atattagtaa gtaataaatt gtatcataaa tattgtcaaa atgaggtgaa
    12601 gaaggtgaat cttacaaaag taaaaggaaa tacctttttt ataaaaggtg gcacgaatac
    12661 aggaatatat gtatttgaag ataacacagc acttatgata gacccagggt tatgtggttc
    12721 aagacctagg agagtaaata aagtattaga tgaaaataat atacaggtta gatatattat
    12781 aaatactcat ggtcatgatg accactatgg agcttgtaat caatttaaag aatattataa
    12841 agatgtgtgt ataatgtcat ctgaatacga taaattatat atagaaaatc cagaattgtt
    12901 ttcaaagtat attgttgggg gaaagagcaa tgtatttatg gataatatct taaaaaataa
    12961 aatgttggat gatataaaaa tagataaatc tatagaagct ggaaaaattg aattaaacaa
    13021 tgaaatgttt gatataattg aatttcaggg tcatacacct ggtagtatag ctgttttgac
    13081 gaaggataat gtgatttttg ttggcgattt attgatagga gatgaaattc tatcaaaata
    13141 cgatttcttg tttctatatg atatacaaga acaaataaat tcattagaaa aattaaaaaa
    13201 tatagatttt gagtacttag ttttagggca tggcaagcaa gttatatcga aagaagattc
    13261 ttataaatta atagaaaaac atttgaatgc tttaaaaaaa tatttatatc aaataagaga
    13321 gttgcttgta gaaccgaaat ctttagaaat tttattaaaa aacattataa ataacaataa
    13381 tttaagcaat aattataaag agtatcattt ttttaaatct tcgttagtat ctgtcataag
    13441 ttacttagtt gatttagaag aaatagggta tatattggaa aatggagaat tgctttacta
    13501 tacaaaaaca aaataaatta tgataaaata aagaaggtca taaaaaacaa ataaagggtg
    13561 agcaatatgg aatacgaaaa atggaatgag atactagcac cttatgaaca tgcagtagag
    13621 gaattgaaaa taaaatttaa aaatataaga aaagaatatt tagcaaaagg tgaatattct
    13681 cccatagagt ttgtcacagg tagaacaaaa aaaatatctt ccataatatc taagttaaaa
    13741 aggataaatg caaaagatat agaaactgaa attgatgata ttgctggtat aagaataatg
    13801 tgccagtttg ttgaggatat atatgctata gttgatttga taaaagtaag aaatgatatg
    13861 actataatag gagaaaaaga ttatattaca aattacaaag atagtggtta tagaagctat
    13921 catgttataa taaaataccc tataaactcc attgcgggtt caaaggaaat tatatgtgaa
    13981 atacaaataa gaactcttgc aatgaatttc tgggctacaa tagaacattc attaaaatat
    14041 aaatatgacc attacatacc tgagactctt gctgaaaggc ttagaagagc atctgatgct
    14101 gcctttcttt tagaccaaga aatgagtgaa ataagagagg acataatgaa agctcaggct
    14161 atgtaccaaa tgaagtctat agctattaga gatactttaa atagaataca agagttatat
    14221 gacttgggtg atacacataa agctatgcag taccaaagaa aacttgatag aactgaaaca
    14281 gataaaaata taactgaaat tttggaatta aaacaagaaa tagatatatt attagagcaa
    14341 tataaggata ctaaggtaga tgataaagta gttcagtaaa aactacttgt agaaaatttg
    14401 ctaaatatta atggggctgc ctcttaatgg aaaataattt ctaaaaagag acagcctatt
    14461 tagtgtaatt aagaaaggaa tgatattatg agtttgtatg ctataggtga tttacatttt
    14521 tcaacatcag tcaataagcc aatgaatatc tttggaagta attgggatgg tcatgaaaag
    14581 aaaattatag ataattggaa agaagttgtt aaggaagaag atatggtctt agtactagga
    14641 gatacatctt ggggaattaa tttatccgaa gctaaaaaag atttagatat aataagcaaa
    14701 cttccaggtc agaaaatact tataaaggga aatcatgatt actggtggac aaccgttaca
    14761 agtttaaata agctatatga agacatgcgt tttattcaga caaattttta tgaatataaa
    14821 gattatgcaa tttgtggagg taggggctgg atatgtccta atgatgttaa atttgatgag
    14881 actgatgaaa aagtttataa aagagaagaa catagactta gactatcatt agaatctgct
    14941 cgtaaaagtg gtcactcaaa aataattgtg attactcatt atcctcctac gaatgataaa
    15001 ttggaagaat ctttatttac aaagttattt gaagaatata atgttgaaaa ggtgatttat
    15061 ggtcacttgc atggtaaaga atcttttaaa atgggattga aggggattcg aaatggagta
    15121 gaatatacat tagcatcttg tgactataca gaatttaatt taataaaagt tcacgattaa
    15181 aaaacataaa ttggtaaata ctataagata catgattaaa aataaatatt tgagggattt
    15241 cggttttgag aaaggagaca cgacttatga ggtggatatt agtttttata tttttatatc
    15301 ttattccttt agtagtgata ttcagaaatt acaagaattt taagaggtct tgtatctaca
    15361 gtagtattta tgtggtatta gctacaacaa taatgataag taatatttat atgagtggac
    15421 ttaataaaat aaaagagtct atgtattatc acaattatat ttctgatgag acatatgatg
    15481 aaaagtatgt atcaaatttt aaggaaaaag aaagtactga tttaaaacag gataataaaa
    15541 agaatgaaga aatatcaaat aataaaaatt taaacaacaa acaa
//
LOCUS       DAELNY010000079        15534 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.079, whole genome shotgun sequence.
ACCESSION   DAELNY010000079 DAELNY010000000
VERSION     DAELNY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.079"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(96..956)
                     /locus_tag="KPE55_002118"
     CDS             complement(96..956)
                     /locus_tag="KPE55_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG0493181.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELIDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1273..2601)
                     /locus_tag="KPE55_002119"
     CDS             complement(1273..2601)
                     /locus_tag="KPE55_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0493182.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2749..3078)
                     /locus_tag="KPE55_002120"
     CDS             complement(2749..3078)
                     /locus_tag="KPE55_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0493183.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3102..4391)
                     /locus_tag="KPE55_002121"
     CDS             complement(3102..4391)
                     /locus_tag="KPE55_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0493184.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4407..4733)
                     /locus_tag="KPE55_002122"
     CDS             complement(4407..4733)
                     /locus_tag="KPE55_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493185.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4868..5497)
                     /locus_tag="KPE55_002123"
     CDS             complement(4868..5497)
                     /locus_tag="KPE55_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493186.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5712..8369)
                     /locus_tag="KPE55_002124"
     CDS             complement(5712..8369)
                     /locus_tag="KPE55_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0493187.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKGIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8728..9672)
                     /locus_tag="KPE55_002125"
     CDS             complement(8728..9672)
                     /locus_tag="KPE55_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493188.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9665..10690)
                     /locus_tag="KPE55_002126"
     CDS             complement(9665..10690)
                     /locus_tag="KPE55_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493189.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10765..12333)
                     /locus_tag="KPE55_002127"
     CDS             complement(10765..12333)
                     /locus_tag="KPE55_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0493190.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12446..13357)
                     /locus_tag="KPE55_002128"
     CDS             complement(12446..13357)
                     /locus_tag="KPE55_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493191.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13341..14327)
                     /locus_tag="KPE55_002129"
     CDS             complement(13341..14327)
                     /locus_tag="KPE55_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493192.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14687..15088
                     /locus_tag="KPE55_002130"
     CDS             14687..15088
                     /locus_tag="KPE55_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0493193.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15091..15489
                     /locus_tag="KPE55_002131"
     CDS             15091..15489
                     /locus_tag="KPE55_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG0493194.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4896 a         2705 c         1729 g         6204 t
ORIGIN      
        1 ttatatttta ttaattttta ctacttgtaa attattatct tatacaatga atttgtatta
       61 tataataagt atttttagga ttctgttaca taatattatt taaatattct tctatatttc
      121 tcttcttctt cttcagtaaa cacattaaat acaactttta aatttttttc aggattttct
      181 tttagccata gtctaactct acttacagca agattagctg cctctttttt tggataacca
      241 aatacacctg tagaaattcc acaaaaaaca atatttttta tttcatcaat ttcttttatt
      301 gtatttaaac atgatttata acaatgaagc agttgctttt cttgctcttt acttggctga
      361 ccaccagata caataggtcc aacagtatgt actacaaatt ttgcaggtaa acaataacct
      421 ctagttatct tagcatctcc agtatattct aaatgccctt gtttttttat tattttgtca
      481 caatcttctc taagtctagg acctgcgcat gaatgtatct cattatccac gcatagatgt
      541 aatggttgta aacaccctaa taacttgtta tttgcagcat ttactattgc atctgctctt
      601 aaattcgtta tattacctct ccatatggca atcccttcct ctacttcttc tatatcattt
      661 acatctatta actctctttc ttcacattca atcaacaata aattatgtaa catattaagt
      721 atctctggag caatttcttt tggtggatat acattcaaaa gtcctctaag tatatctcta
      781 ggcttaattt catcatttga taaattgtat attttagaag gaacttcttt tgaaaaatat
      841 gcaattaaag tgtttatatt ttttcttctt tgttcgtcag ttagaggctt gatagtttta
      901 tcaaaatcct caaataaatt tacatcatca gcataatctc tccacttcat agccatctct
      961 ccattcttgt attttagact taaaaataat tatgtattta tgtacatatt tatttatttc
     1021 tataaacacc tacataaatc ctgttaaaag tgtaaatttt ttattatgca acttctctgg
     1081 ttatttctat tgattgatag taaattattg tatctaaatt taatccaact aacaagtaac
     1141 taaacctact ttcctaactg caaaatacac tatgttttcc acttgtatct catttgtaga
     1201 taaataaagt ccctacaaat acattaacta agctatgtac taaactgtag gaactttaaa
     1261 aatatattat tcttaattaa gttttggcca ataattttta tttgcttcta ttaaatcatc
     1321 tagtaattct tttgcaactg ttgcactagg aactgtctta gatagcataa gtgcttgcca
     1381 caatttatta taagacttct caatccaagc atcaactact aatttttcaa ctgctaattg
     1441 ttgttctatt aaaccttttt ggaatgttgg tattttccct attgataatg gttcataccc
     1501 gtcttttcca actatacatg gaatttcaac catagcatca tcttgtatat tttctatagc
     1561 tccattattt tgaactatca ataacatacg ctcctgtgta ttaaaagcta gagctgtagc
     1621 taaatccact ataaactctg catgtattcc tatcttaagc ccactgccct tagaatgtcc
     1681 ttgcttttct atcaatgcac atgaaccaaa aacttctttt tctcttccat caataacttc
     1741 attggcacga gtataattta catcagaatg atttacaaca taatctggaa atagatagta
     1801 ctttaagtaa ctacttggta ttgtagttaa atctgttttt actatttctt tagtaaataa
     1861 attagtttca aaccaactat ctttcttgtc atttttcatt ccttcttcac catttataaa
     1921 accatattct tttatatgtt ctactaattt tggtaaaagt tcatttcctt ttttatcttt
     1981 aattgaagta taccaaccaa agtgatttaa tccaaagtat cttatatcca tatgtttacg
     2041 agatttgagt cctgcaatcc ttgctatatt atgttccata cctattggca tatcacaaat
     2101 atttattacc ctagaattag gtctcaatct tctaacagct tccgctatta ttgatgctgg
     2161 attagaataa tttaacatcc aacaatttgg agaatacttt tccatataat ctatgttttc
     2221 aagaacacct tctatagacc tcattccata agctattcca cctggtccac aagtttcttg
     2281 accaactact ccatgtttta gaggtatctt ttcatctagt tctctcattt caagttttcc
     2341 aactcttata tgagctaaac aaaaatctac atctgtataa gcctctttag ggcatgttgt
     2401 tgctaaatac tctatattag ggtccttttc ctttattagt atttcacaag ctttagctac
     2461 cttattttgt ctttcttcat cattatcata taatttgatt gctcttaatg gtaacctatc
     2521 taagttatct agcaacatta atatgatttc tggggtatat gtacttcctc ctccagctat
     2581 tactactgaa aatttttcca taataatatc tccttttcta tttaaaaatt tactgtattt
     2641 ataaataaat ttataaatac tagtacttta aagtaaaagg gagttcattt attaaatcta
     2701 ttgatttaat aaactatagc caaacttcaa gcactattta caagaaattt aattcttatt
     2761 aattcttggg tccatcatct caaatatatc tacaagttca gatactaaag aatgagcaaa
     2821 taatgcattt gaataatggt cttcagagtg cacaactaaa aaatcagtcc cactatcttc
     2881 actattcata gcatttgata gtaattctaa atgatgcttt gatgctgttt gtattttctc
     2941 tgaggctgaa tttaaaaggt gttttgcttt attgtagtca ccttttttta tttccttaag
     3001 agacatcatt acatcacttt tagcatctcc tgcttctgat attatactaa atactttttc
     3061 ttccatttca ggtaccatat ccttctcctt ctaatgactt gttaaaaatc aaagctatca
     3121 tcatctaatg ttgtagctaa ttctgcattt ttatttaatt cttccttctc cattatctta
     3181 aagaatggat aatatatcaa ggtcataagt attatacttg caaatactaa tacgattgcc
     3241 ttccaatcta atgtcatcaa aaatgctgca acccctggag gtaaaaagcc tggtggttct
     3301 gcaataggta atcctactaa tcctactttt aatgccataa aattaagtgc tccaaccaat
     3361 acaggtccaa atacaaatgg tacaaacata tatgggttca taactattgg tgttccaaat
     3421 attactggct catttattcc aaatagtgat ggtaaaatca caacttttcc catactatta
     3481 aaactcttac acttacttct tgacataagg tacactaatc ccaaagttat ccctgaacca
     3541 gttacttgca agaaaccata gtaaaatcct attgtaaaca catattttgg gtctcctcct
     3601 gctgctatca gtgttgcatt ttctgccata tatgtagctg ctattggcat ccatatactt
     3661 gaagttattg ctgctccatg aagcccaaag aaccataata attgagttat aaatagtatt
     3721 atagttactg caaaaacatt atctacactg cttatagctg gtgctaagaa attcattata
     3781 atagctggta gtggttgacc tgagacattt ttagttattg ctgctattgc tgtaaataac
     3841 attatacata ctatcattgg agttaagttt ctaaaacttt tatttactac actaggtact
     3901 tcttttggaa atctaattac gagattttta tcattcataa acttagtaat atcaactact
     3961 aaaagtgcta ctaatatact tacaaataaa ccttgtgcat ctaaataact tgtatctaat
     4021 acaccatcta ctacttgtcc acatacaaca agttgtgcaa caactgctgt catacctatt
     4081 tctacatgat taagattata ttttttagct agtgcctgag ctataaaaaa tgctgaatat
     4141 atagataata aactcatagt aaatttatta gggataagta atgtatccat attttcagat
     4201 aataatgttt ttgcacttcc ttcaggtaac atattgttta atgccataaa taacatactt
     4261 aaagaaccaa caattattat tggggtaatg cttatcatac cttcttttat agcttgaaga
     4321 tgcctttgtt tttcaatctt ttgtgctatt ggaagaagaa atcttcttaa attattttga
     4381 agtgcttcca tttttattcc ccccaattat ctatttttat ataattcgat tgccgacttc
     4441 ataacagaag gagcatccat acgaccataa tgcttagtat ctattaaacc aactgctatt
     4501 ttgtaattct tacaagcttc aactacatca tcttccttaa acctaatttg tggtgctaca
     4561 agtactacat ctgtatcatt agcttcaatg tatgatttta aattttcaaa tgatgttgcc
     4621 tctatattta tagttttagt ttttaacttt tcatgctcac tacaaaaact tttcatcttt
     4681 tccacaagta cacctgttga agctccattt gcacatacta ataaaatatt cataattacc
     4741 ctcctacaat atctaacctc ttttgttttc taatttttta tcagccaatt cttactctaa
     4801 ttcctatcag tttttacttt aattcttatt attttatttt aattcttatt aatatttact
     4861 ctaattctta gtactgccaa tgattaaact tgaaagccgt atctataact tcttcttgct
     4921 tatatttttc taatattttt aagtatcttt ctttataatc attatctact tctttttctg
     4981 gtattactat actagcttca tttaaaaagt ggaatgaatc ccttcccatt tctaatcctc
     5041 tatatgtatg cttgtcaagt gccacgtcca taacttctgg aactttaccc attgcaaatc
     5101 cttttataat tatatttttt aataaatcac ttgatctatc tttctcacat gcacaaagat
     5161 atcttattgc atgtatgaaa aataaaggtc tgtctccatc tgcatatgaa tattcctttc
     5221 ttacttgatt taaattattt atgattatta cagctgatgg gtcccccatt cctatatctt
     5281 caactgatat tgtaagtatc cttctccata gcttatcttc taattctggt gatgatatat
     5341 atagttcata agcaaattca caagcttctt ccaccatatt tcttcttata gctttttgaa
     5401 atgacgatat tatttcgtct gcactaaatc cgtttcttgt agtcattctt gcccatgggt
     5461 catttgcctt tttatctctt aatattttat cttgcatatt atttactcct ttttatttaa
     5521 attttaatta tttatattgc tcatttaata atataagcaa gtattatgcc agttttttta
     5581 gacactttaa aacactgtta tttcaatcat ttttatcatc aaaacttaga tactttgtct
     5641 aagtatattt atatgtgtct aatattaata ttattttaga ctaaaaaaaa ttaccaaata
     5701 agtttttata ctcattggta atctttttcg attatttctg catattctaa tattaataat
     5761 atttgatagc actctttttc attaatttct atattatatt ttttatctaa ttgagatact
     5821 atatctttta ttttgcttag actcttgtga taatcaagtg ttttttcttt atcaagcttt
     5881 tcaatttctc tagtatttcc aatcaaccca tctaccatga aagctaaatg catcataagt
     5941 ccaattactt tatcgtattt cttttctata ttaaatacta aaaacaattc atcagacaca
     6001 tattttatac ctttaaatat cctattataa ttagagcttt ttaaggatgt ctttaaacct
     6061 tcaactacat catcataacc tatacttatc tttataatat tttttaattt attgatattt
     6121 tcatccttat ctatatcttt tttgctaata tagtcaattc ctctaatatt aattctttgt
     6181 tcattgatta ctgcaacaat attactactt tctttaagtt tcgtaatatt taaacttaaa
     6241 tctttttctg ttttatatgg gacagatata atatctattc catctaagta cctactcatc
     6301 ttttcttcta atatttttct tgttttatta tctacacctt ctgaaacatg tgatgctata
     6361 ataattgttt tagacagatt ttctttattt tgaatttcat tagttccaaa atagtacttt
     6421 ctcgtgttag ttatagagtt gtatacgtca tttaaaggta atcccatatt ggccttctga
     6481 actgcttcta acacagttaa tgtcgtcacc ataggaatta ctcttactcc acactttttt
     6541 tctttcttta taatttcatc aaaagagttt aatgaaccca tatctgtaaa tagtaataca
     6601 tctttattgt ctatattatt aattaaattt ttaaaatcta caattacttc atcatagctt
     6661 ttatctaact tcatatcgaa agaaatagca ctgcactttc caataatatc accaactgct
     6721 tttaccatac tacttgctgt agtttctcca tgcatagcaa ctataatatt aatgttattc
     6781 actattttat tttgttcatc ttgtttagct tgtactatga ataatgtaat atatgctaca
     6841 gtttctatag ataaaaataa atcaaattca ttttcaatta ttccaactat ttttaatgct
     6901 gttttaaatt cacttctgtt atttcttcta atttgattca aatctatgtt gtttgtagag
     6961 ctacttttat ccaatacgct ttttatataa attgccattg acttaaatat tgattcactt
     7021 atgtctattt cttcatcttc tttaataatt tgtcttattg attcacacat gcttttaaac
     7081 ttaacattat taaatagaga agcatctgct atattcattt ctacagattt attaagatta
     7141 gtatttactg cattaataat atttatatca ttgtagttaa taacttcttc ttctcctaat
     7201 gacgtaaata caaaatattc tattccatgt gttataactt catctgcttt aatagacttc
     7261 tctctataat catcactcac ttcatttata gatttatcaa aatatttttt ctctatataa
     7321 actggttctt tattatctct tttatatctt agatatccat ttgcaacagc taattttata
     7381 tcattttgaa gttgacctac attattttta caatcataaa gtaatagtga tttaattatt
     7441 tctttatgta cttttattga aatagaaata ctttttgatt cttccttgaa aaaatttttg
     7501 actaattcca ttctttcaat cattgtccta tctctcaatg atggtatttc tattttcatt
     7561 ggtattcttc tattgaatgt atctaatagt gttgatgtcg gattttctgt agtagcacat
     7621 attattaata ccttagactc tctttctttc gttgcatctc ctagtctttt aaatgttccc
     7681 ttatctataa aagtaaacaa catttcttga ccttctgctg gtagtctatg aatttcatct
     7741 aaaaatagta tccctccatt tgcaatttcc aaaagacctt ttctggaaga ttctgcacct
     7801 gtataagctc cttgttctac cccaaaaata tgtcccataa gtaattgtgc attattagca
     7861 taatcagcac aattaaatgt tacaaaagga gctttatttt ttctaattcc actatctata
     7921 gaataatcgt acatatatct agcaatcata ctctttccta ctcctgtttc tccatagata
     7981 atagtatgaa gtccatttgt aggatagagc attgcgacct tgcatttttc tattataggt
     8041 cttaaactat gttctttacc tattagtgtt tcaaaactat ctttttttat tttgttttct
     8101 acttttgttc ctttttttat gccaagcaac tcactttgtg tatcaagcaa ttcattcttg
     8161 ttaaggttaa gatttgcagg gatataatac acaggtcttc cttttatctt cgtaaccata
     8221 ccctcattga ataattggtt taaatatgta cttatattgt ttcttgcaat tcctgtcttt
     8281 tctgataatt ggttagctgt aatccctttt tcattttccc tatatgatga ttctaaaact
     8341 ttcattactt tatctttttt atatatcatt acaagctccc ctacattata gtattttatt
     8401 aactaaaacc cctatacaca ctttattttt taataaactc ttattcttta actaaggtta
     8461 taatttatca aattttcctt actttaaatt atagaaaatt attgtagaaa aaataagatt
     8521 attgctcatt ttttctacat tactattttt aattttatag tactttctta acaacatact
     8581 ttacttgttt ttaattttat tatatttgaa tttattatat ttgaaaagta gaaataaaaa
     8641 accctatttt ggagtagttt taaaatttct ccaaaaatag ggttttagtt tgtatttcaa
     8701 ataaatacct gttcaagtat ttttatacta aacaactttt aaattttgtt gatatttatt
     8761 tatttctgat ttagaagaat atacaaaatg tccatctgaa acctcaatcc aacttaatga
     8821 cttttcattt gaacaatcat atccattaga attatactct attctatttc ttgacttagc
     8881 atatctaggg tcaggaacag gaattgcaga aagtagtgat tttgtatatg gatgaatagg
     8941 attattatac aattcatttg aatctgccaa ttctactaat tttccattat acataactcc
     9001 tatacggtca ctaatatgtc tcaccataga taaatcatga gctataaata aacaagttaa
     9061 attcttttct tgttgcaatt ttttaaataa atttactatt tgtgcttgta tagacacatc
     9121 caaagaagct ataggctcat cagctattat aaattctggg tctactgaaa gagctcgtgc
     9181 tatgccaata cgctgtcttt gtccacctga aaaatcagat ggatacttat ttgcataact
     9241 tctatcaagt cctactagct ttagcatctc atatacttta ttcattctat ctgttttatt
     9301 tttacagata ccttgaattt ttagtggctc tgaaattata tctgcaattg tcatacgtgg
     9361 atttaatgat gaagtagaat cttgaaaaat aatctgcata cttttgttta catctttttt
     9421 tataaatttg tacgtttttt tatctgatat gcaatttcct ttgtaaatta cttctccacc
     9481 agtagattca tgtagtctta ttatagtttt accagttgta gattttcctg aacccgactc
     9541 tcctaccaat ccaaatatct cacccttata tatatcaaaa cttatatcat caactgcttt
     9601 tacagtagta cttttgtcca aatgaaaata ttgctttaag ttttttattt ccagtatttt
     9661 ttctctattc attagaaatc acccttccac aatttactct aactggtaca tctacttcag
     9721 gtgcatctgg gtgcaataac caagttgctg cactatgtgt atcattaatt ttaaacattg
     9781 gtggctcttt ttcataatct atcttaagtg catttttatt ccttattgca aaagcatctc
     9841 cttttggtgg gttcaataaa tttggaggca ttccaggtat attatataaa tagtcatcct
     9901 gcgaatctaa agtaggtaat gaacccaaca acccccaagt atatggatgt ctagggtcat
     9961 aaaatatatc ctctacagtt cctatttcta caatctttcc tgcatacata actgctattc
    10021 tatcagctat agttgcaact actcctaaat catgagttat aaatattatt gacaaaccta
    10081 ttttatgttg taaatccttt attaaatcaa ttatttgagc ttgtatagtt acatctaaag
    10141 cagttgtagg ttcatcagca atcaatacat ctggattaca ggcaagtgct atagctatta
    10201 ctatacgttg tctcattcca cctgaaaaat gatgtggaaa ttgtttaaat cttttctcag
    10261 gattatcaat tccaaccaat tcaataagct ctatggctct cttttttgct tcacttttac
    10321 ttataccttg atgaatgatt acagcttctg caatttgctt tcctattgat atagttggat
    10381 tgagagatgt catagggtct tgaaatatca ttgatatatt ctttcctctt atatcttcca
    10441 tctctttttc agacttctta accaaatcac ttgatttaag taaaacctct ccatttttaa
    10501 tatatccatt gtatgggagt atcctcatca aactcttaca cagtacagat ttcccacaac
    10561 cagattctcc aactatagcc aatgtctcac ctttttttag attaaaagat acatttctta
    10621 cagcctgaac ttctccttct tctactttaa aacttacaga tagattatta acctccaata
    10681 aatgttccaa aaaccatcaa cccctatgct attcaaacct tagggatata tttttatccc
    10741 taaggtttct aattaatttc agtattattc aattgtccag tcagctatgt tccagaatat
    10801 acctacacca tgatgaccta aaactgtatc aggtgttaat ccttttatgt ttggttttcc
    10861 aacatatatt gcatctatat aagcaataaa tgtataaggc atatccttag tcatttctac
    10921 ttggaattgt ttatataact ttaatttttc atctttatct tctgtttctc ttgctttttg
    10981 aagtatttta tctatagtag gattagagta agcactgtaa tttgcacctt tatctgttcc
    11041 aaatacttta tatgtatggt catctgggtc aaatggactt ccccaaccta ttaaatgagc
    11101 atcttgatta gcccaatctg tttcagttac aacaacagct ttagcatcaa caccaatttc
    11161 ttttaactgt tgtgcacata ttttagccat atcaactcta acttggtcac tctcacctgc
    11221 tgttatctcg aaagctaact tagtaccttc tttttcatag attccatcag aacctaattt
    11281 ccaacctagc ttttcaattt cttgtttagc tttttctgga ttatattcaa acttttctat
    11341 atcaggatta ttatatggtc ccatttgtaa aggtgaatat gctggtacac catgtcctaa
    11401 taatacactg tcaactattg cttttctatc tattgcatag cttaaagcat ttggtaatcc
    11461 tttagctttt ttatctttaa agaatttaga attaaaattg tataatatac ctctatagtc
    11521 ggctgttttc attatattta ctttaaaatt cttttcgtct ttttcaaagt tagacatatc
    11581 tttaggagtt atttgagcta ggtctaattc tcctgatttt aattgcattg ccttagcctt
    11641 atcatctgga acaatcttaa agactacttt atctaatcct ggttctttta caaaataatc
    11701 actattctta acaagtgtta tactttgacc tttgtcccat ttttcaagtt taaatggtcc
    11761 agtacctatt ggtttttgat taaattcatc agtagctata tccttacctt ccaaagcatg
    11821 ttttggtaat actcctactg ttaaataatc aagcattgct gtatttggag cttttaaagt
    11881 aatttttata gtattatcat taaccacatc aatttttgtt atatcttcat aatttgatgc
    11941 tatctcagaa gcattatctg gattcattat agtttctatt gtaaacttaa catcatttgc
    12001 tgtaaatttc tctccatcat gccacttaac atcatctctt aaattaaaag tatatgtatt
    12061 tgttgcctca tcaaatttcc agtcctttgc tagacaagga accaccttat tattttcatc
    12121 atgagctgtt aatccattaa atattaacga atttatttct ccatgttcgt atagtgcagg
    12181 atttatactt gtatagtcat tacttccata aacaagcacc tttccatctt taggcgtatc
    12241 tgccttctta tctttgtctc ctccactaga acaacctgcc attagactaa gtatcattac
    12301 taaacttaaa acttttaact tctttaattt catgatgctc ccccttttgc tttttataaa
    12361 atatttttca aagctttgac cccatttatt taaatagata tttcaaatac ctcagtattt
    12421 gcttatttaa tactcaaatg ctgatttata aattactaga ttttttatta ttattctttc
    12481 ttatataatt acctatattt gttatacaca ccaatgtggt aaccaaaaat atacccggaa
    12541 tcagtataat ccaccatcta tttgaaagta atgcttcctc tgacaaagat aacatacttc
    12601 cccaagaaac aatttctatt ggtaaaccaa ttcctaaaaa gctaagtgtc gattcagtac
    12661 caatagctgc gcctatgttt gttacaacca tgaacattat tgatgccaca aaatttggaa
    12721 gtaaatgttg ctttaataca tagaagaatc ctcctcccat gctcttagct gctagtatgt
    12781 attcgctatt ttttatttgt ctaacttctg ttctcactat cttagatata ttcatccaac
    12841 ttgttatccc tattactatg gacatagata ctggatttga atttccaagt atagcttgta
    12901 caaatattat aattaatatt gaaggtatac taagtatgat ttctgtaaac ctcatcattg
    12961 catcatctac aagttcagat gctgaaccac ttatactacc ataaacaatt cctataacag
    13021 ttgatattac agtggaaaat agtcctataa ataaagatat ttttcctcca taccatatca
    13081 tagaatatat atctctaccc attgagtctg ttccaaataa aaaagtttta tttggagcta
    13141 aattagaact tgctaaatcc atgtatgttg gttcatgtgt cattataaaa gatgagaata
    13201 cacttccaat gactatgatt gataaaataa taattgatat atatggtaat tctttaattt
    13261 ttttagataa ggttttcttt gtttctatat gttcatcttc tgctactaaa atataatttt
    13321 ctcctacaat ttcaaaatca ctatattgtg gcttcattag tatcacctct atcatatttc
    13381 attctagggt ctattttatt gtttataatt tgagctaaca tattcccaaa tactactaaa
    13441 gcccctgtta ttaagcataa aactaataac atattataat catgatactt tgcactttca
    13501 aagcaaagtg ttcctaaccc aggataagaa aaaacttttt caactacata agttcctcct
    13561 agtatatgag gtatcgaaat ggccataata ctaatatatg atggcattat atttctcagg
    13621 caatgcttaa aaacaatagt tctattattt aaaccttttg ctttacataa aagcacataa
    13681 tcttctctta tttcctctag taatttattt cttatcatat atgtgtaata ccatagatgg
    13741 cttaatataa gtactgtaag aggtaagatt agatgtgata tcctacttaa tatactggac
    13801 tcttctccca ttgaataagc accactactt ggaagtatac ttaagtttat actaaatata
    13861 agtatgagta ctaaggcaac ccaaaatgat ggaatacagt tggttattgt tcctaatttg
    13921 catatgattc tatcaatgag cttatcttca tgtaatgtac aaaatattcc taacaataag
    13981 gctagcacaa aggtaagtat atatcctgaa ccacctaata ttatagtgtt tatatagaca
    14041 tcatttataa cagaagtaac attttgttta tatttaaatg aaattccaaa ttcccctttt
    14101 gatgcatttg aaatccattt tatatattga ctgtatattg gttcatccaa accaagtttt
    14161 ttcatagcat tttctttttc ttgtgtactc attctttcta ctccatcccc atagtaagag
    14221 attaatgggt ctcctggagc aagtcttgcc atgtaaaaaa caataaaaga taataaaaac
    14281 attactaaaa taaactgcaa caactttttc agtatatcct ttaacaaacc ccaacccccc
    14341 tttgttgcaa taaaaaaaac cacataaaag caaacgtaaa gtaacgttaa aaccttcgtg
    14401 gttccattat cattaaacaa aattaaattt tctatattta tctatgagtt aattttagca
    14461 tcatttatta actgtgtaaa caaacttttt aaaaattgtt aaaatttcat caaattattt
    14521 tatttatacc taaaataaaa tttttatatt ctattcttca ttattttcta taaaattata
    14581 aaattttagg ttgactatat aagaaaagta tattatatta gtttgtatgc aaaccattta
    14641 agaggtgatt ttattgaaaa gtaattatga ctgttttaga atagccatgc tcttaaaaga
    14701 actttattct aaaactatgt acacagtaga agaaaatttt aaagaaaatg gattaacaca
    14761 ccaacagatt atagttataa aactgatagc tcataatcaa gaacttacaa tatcacaact
    14821 atgtgatgaa atgtctttag ctaaaggtac ggtttcaggc ataatcagca gattagaaca
    14881 gattggatat atagaaaaat tcaagaaatc taatgataaa agaaatacat acgttaagtt
    14941 tacaacaaca ggatttgaat ttgccacaaa ttttaaaatt aaaatgcaag aaagttttga
    15001 tgatattttt aaaaattgtg atgaaaacga attgagtgac ttagtaaaaa atcttagaaa
    15061 tatattggca aaagtaaagg agagataatt atgaattata aattaatact tgcaatagta
    15121 tttataacat cagctttaat tttctacact ataggtgtat ttggagaaag aaaggcaaaa
    15181 attttaaaaa agaaacatgt aattatattt tggcttggtt ttatatttga tactttagga
    15241 acatttacaa tgagtaacat agccaacagc catacttttg aagtaaaatc tgctttatca
    15301 caaaaccttc atagtattac aggtcttctt gcaattgtgc ttatgctttt tcatgcaagt
    15361 tgggctactt ttgtcctata caaagatgat gaggaaaaga aaaaattctt tcataagttt
    15421 agtatagttg tatggactat atggttagtt ccatatttta taggtatgtt tattggtatg
    15481 gctggatagt aaaataaaaa gtgtgaaaga aggatgtagg taaatttgta taag
//
LOCUS       DAELNY010000080        15473 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.080, whole genome shotgun sequence.
ACCESSION   DAELNY010000080 DAELNY010000000
VERSION     DAELNY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.080"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            198..962
                     /locus_tag="KPE55_002132"
     CDS             198..962
                     /locus_tag="KPE55_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0493195.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            1351..2664
                     /gene="prdC"
                     /locus_tag="KPE55_002133"
     CDS             1351..2664
                     /gene="prdC"
                     /locus_tag="KPE55_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG0493196.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      3067..3152
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            3734..5842
                     /locus_tag="KPE55_002134"
     CDS             3734..5842
                     /locus_tag="KPE55_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG0493197.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPLDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQMPDGEKPSDSQTPDVEKPSDGQMPDGEKPLDEQIPEEEKPLEEE
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSEDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCDKLIEIAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPV
                     PPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSY
                     VFISVIAICGLAYQCKIKRN"
     gene            6249..8009
                     /gene="prdR"
                     /locus_tag="KPE55_002135"
     CDS             6249..8009
                     /gene="prdR"
                     /locus_tag="KPE55_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG0493198.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            8438..10318
                     /gene="prdA"
                     /locus_tag="KPE55_002136"
     CDS             8438..10318
                     /gene="prdA"
                     /locus_tag="KPE55_002136"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG0493199.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            10399..10683
                     /locus_tag="KPE55_002137"
     CDS             10399..10683
                     /locus_tag="KPE55_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493200.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            10721..11446
                     /gene="prdB"
                     /locus_tag="KPE55_002138"
     CDS             10721..11446
                     /gene="prdB"
                     /locus_tag="KPE55_002138"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:11171..11173,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG0493201.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            11526..12284
                     /gene="prdD"
                     /locus_tag="KPE55_002139"
     CDS             11526..12284
                     /gene="prdD"
                     /locus_tag="KPE55_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG0493202.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            12299..12766
                     /locus_tag="KPE55_002140"
     CDS             12299..12766
                     /locus_tag="KPE55_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0493203.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            12784..13266
                     /locus_tag="KPE55_002141"
     CDS             12784..13266
                     /locus_tag="KPE55_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0493204.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            13286..14293
                     /locus_tag="KPE55_002142"
     CDS             13286..14293
                     /locus_tag="KPE55_002142"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG0493205.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            14413..15294
                     /locus_tag="KPE55_002143"
     CDS             14413..15294
                     /locus_tag="KPE55_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0493206.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         6176 a         1842 c         3004 g         4451 t
ORIGIN      
        1 atatacagaa aaataaaggt aaaaatgtaa aaaatataaa aaaattaaat aaatgagtat
       61 tttagagagt ttaattaggt caatataggt tttatttttt tagttattgt cttataaatt
      121 aaatattaca gtactttttt gagctgaaaa aaaataaatt ttaaatatag aagaaattat
      181 ttcaaagggg gaatagtatg aaaagagaag ttaaaaggac taaagctttg atatatagtg
      241 ttttattttt ttcattatta ttagtgttaa tatttgtttt tgtttatgga ataaaataca
      301 tatgttctaa atctgacaat tcaaaaatgc ctatatataa agttgataca aatgagaaaa
      361 aaatagcttt gagttttgat gtagcatggg gaactgataa tatggatgat attttaaaaa
      421 ttttagataa acataatgta aaagctacct tctttttagt tggtagttgg gtggatgata
      481 atgaagaaat tgtaaaagct atagatgaaa aaggacatga aattggaaat cattcaaaca
      541 ctcatgccaa tttgaaggaa atatcaaaag aagacataac aaaagagata gaaactacat
      601 cagaaaaaat atataatata acaggaaaaa agactaattt atttagacct ccttttgggg
      661 acgtaaacaa taaagctatg gacatttgta gtgatttagg ttataaagta attaagtggg
      721 atgtagattc tatagactgg aaggagcttg gaccaaatca tgtaattgaa aaagtaataa
      781 aagaatctca acctggttca atagttttat tccatgctaa tataaatgat gtagataact
      841 atttggatac tataataagt cgactaaaaa aagatggata cagtctagtc aaaatatctg
      901 atttgttgta taaagataat tatgtagttg attccaatgg agtgcaaaaa actaagaatt
      961 aataagtaat aaatgtatat tttaagtaaa gttgtgataa attatatagt atatgttaga
     1021 aaagtgtcga taaaaagacg gtgtctattt tttgacactt tttatacatg tatgcaatat
     1081 caataattat agattaaatt gattaaaaaa gtgtctaata attgactttt ttttgatggg
     1141 tttaaaaatt tagaagaatt aatgaatgct gtaagacagt atttccaaag ggtataagtt
     1201 tgtttaaaag ttggcataga aattgctttg ttaataagcg atatgttcta tagaaataaa
     1261 caatatgtta agaccttata atagaaaaat agcattaaca ttatttgaac agcttataaa
     1321 aaaattaata tacttaaggg ggcgtactag ttgaacaaac tatattctat tttattaaaa
     1381 caacacgttg gtggtccaga taagccagta gtaagtgttg gagatgtagt aaaaaaagga
     1441 actctaatcg cagaaccaac tggattaggg gcaaatattt atgccagtgt tagtggaaaa
     1501 ataagtgaaa taaatgacca agcgatagta attgaagctg atgaagtaca agaagatact
     1561 tttgaaccat taaaaggaga aggtatactt gacttaatta aagaagctgg agtagtagga
     1621 atgggtggag caggattccc tactcatgta aaattaaaca tagatttaaa tggtggaaca
     1681 atattagcaa atgcagctga atgtgaacct ttattagctc ataatataaa agaaatagaa
     1741 gaaagaccag aaatagttta tcaaggtata aaatatgcta tggaagttac taatgctgga
     1801 aaaggtatgc tagcaataaa aagtaagcat ccaaaagcaa tagaagcttt caaaaaagta
     1861 ataaagccag gagacaacat agaagttgca gaattagttg atatgtaccc aatgggagaa
     1921 gagagagcaa tagtaagaga cgttctagga aaattacttg agccaactca attaccttca
     1981 gaggctaatg cagtagtaat aaacgtagaa acactaacta gaatagtaga ggctgttgag
     2041 caaaaaagac cagttatatc taaaaatata actgtagttg gtcaattaaa cagtggaaaa
     2101 gaatctatag tgtttgaaga tgtgccaatt ggaacaactg tcggagaatt aattgaaaga
     2161 gctggtggaa taaaaggtga atatggtgaa ataatcctag gaggaccttt cactggtaaa
     2221 gctactactt tagatgctcc aataactaaa actagtggtg gtataatagt tactatgcca
     2281 tttgttaatg aaaagagaaa aatgggtctt ttagtttgtg catgtggacc taatgaagaa
     2341 agaatgagag atatagcaac taaaatggga gttactgata tagtatcagt tcaaaaatgt
     2401 aaacaagctc aagaaattaa aggtgcatta aaatgtgaaa atccaggaca ctgtccagga
     2461 caagctcaaa aatgtattga atttaagaaa gctggagcag aagttatatt aataggaaac
     2521 tgtactgact gtagtaacac agtaatgggt tctgcaccaa aattaaaatt aggtgtatat
     2581 catattactg accatgtaat gagaacagtt aatcacccat taataagaag aataaaaggt
     2641 gacgtaagat taaaaataaa ataatagata aatagaagtt agacatcttt agaagtaaaa
     2701 cttaaaagtt acttctaaag atgtttttta atgtattttg agggcaatat atattgtaaa
     2761 ataaatggaa aaaatagaca aaatatgcta gatattgtat aatgataaca atagctatta
     2821 caattaatat tggacaaaaa taatgatact caagtatgtg gtttgtatta atatgcatat
     2881 tatattaagt caactatccg atagagtaaa aggtttttgt gatatttgaa gatactctat
     2941 agtatctttt ttattataaa aactaatttt tgtcttttat tagataagta tctaggatac
     3001 taatttatat tttattgagt tgttaaaaaa gtgcacttaa aaagttaaaa aagaaaaatt
     3061 aaaaaaccta tttatagaaa ctgtgaagta tatcttaaac ctgggcactt aaaagatata
     3121 tggagttagt agtgcaacct gctataaata taaataggag taacttttaa ttgtcaaaaa
     3181 tatataaaat aaatacataa ataagtaatt aatagaagat agatataaat atatttttga
     3241 aatgctccct gcattcataa cgagtgtaga gtggcagata tctgcccaaa actcctttaa
     3301 ttgctggaag ttcctaaagc taactaaacc acaacataat catgaaataa ggataagtgt
     3361 gatagagtgt gcatttaata agcacttgag gttacgaaag tagaaaaaat tagttagata
     3421 gcataaggtt aaatcctaag tgccttgaaa atggataatc agcaggtaag cttcgaaaag
     3481 aagaaacttc aacgactaga gagaaatctc gtacctacaa gtgtggggaa atggggagta
     3541 cctaatttat ggttaggtaa tgatatagtc tgtgcttaat agaaatatta agaagttcat
     3601 aggagaactg gttaggagta acgaccctaa ttgaacacac taaagatgta ttattgaata
     3661 agattcatat aatatttaat aattttaatt atattaagtg aatacaatat atgtttttag
     3721 tatcaggaga attttgaaaa caaaaattaa aaaatcaagt ataatcagtt tagtaattgc
     3781 ttttagtatg gtatttactg catttacacc aatagtatct tatgctgacg aagtgactag
     3841 caatgataca atacttaatg gagaagaaca atcaaatggt cagacaccag atgtagagaa
     3901 accatcagac ggtcaggtac cagatgggga gaaaccatta gatggtcaga taccagatgg
     3961 agagaaacca tcagatggtc agataccaga tggagagaaa ccatcagatg gtcagatacc
     4021 agatggggag aaaccatcag atggtcagat accagatggg gagaaaccat cagatggtca
     4081 gatgccagat ggagagaagc catcagatag tcagacgcca gatgtagaga aaccatcaga
     4141 tggtcagatg ccagatgggg agaaaccatt agatgagcaa attccagaag aagaaaaacc
     4201 attggaagaa gaaatagtta tagaggaaat gagtttaaaa caagatattg ataaaattct
     4261 tgatatgact ttatctcaaa taaataaaat agtatataac ttttgggaag atgaagaaga
     4321 tgtaaaggca gatgaacaat cagaaattaa ccaagttttt acttcagaag actcatttat
     4381 tagcttatgg tatgacaaaa aagcaaaggt gaaaaattct tgtttactaa aagaaataga
     4441 tggttcagat agaccttatc atgatttaag atttgatgat attacaaaga ctttaacttt
     4501 tgactatgta ataaagggtc ttgtaggagc aagctcaaaa gacgacaaat atgtaataga
     4561 aggtgaagat ggagagcaaa ctgcattttg ttacaataat catttaagac caccaagtag
     4621 taatggaaag agtccatatt taccagcaga agattttaat ggtcaagaaa accaaaatga
     4681 agaagcagtt aaatctatac tatatgcagg ttctgaattt gatggatttg gatataagca
     4741 acaatttaat ttaggcgaag aagaaaatga cataatgact tactcagcta ctcaaagtgc
     4801 aatttggatt attcttggac aaatagatga acaagaaaga cttaagcagt atcaaggctc
     4861 tatagacctt tgtgataaac ttatagaaat agcaaaaact gaagaggaaa gagctgagta
     4921 tcagaagagg aaagaagtag cgataaatac aaaagcttat ttagaagcat tgttaaaagc
     4981 aggtcgtgaa gagttaaagc caaatgatac aggaaaacct agtttgagta atggtttaac
     5041 taagattaac tttgaaaaaa atgaagatgg tacttatgaa acagaagctg tagccttagt
     5101 aggttatagt ggtgtagtaa agttaaaact accagatgga gtaactgctt atgatgtaga
     5161 tggtaatata ataggtacag gcgaagtaga aatttcaacg caacagaaat ttaaacttaa
     5221 gagtgttgga aaaccagatg ccaaagctaa tatttctgct gtatcttatg attatatttt
     5281 tccaaaagct attcaatatt ataaggcagt gcttgattta ggacaaaaag accattcatc
     5341 ttcattacca tctagtaagc aaaatttatt aagttataca atagagaaaa aaaatggtca
     5401 agaagttaac tttaatatag gtctaccaac agatgatgtt gtaaatccac cagtgccacc
     5461 aacagatgat acaaatggac ataagccaaa accatcacca ccaatagatg atacagtaat
     5521 aaatccacca gtaccaccaa tggatgatac aataataaat ccaccagtac caccaacgga
     5581 ggatgcagtg ttaaatccac cagtgccacc aacggaggat gcagtgctaa atccaccagt
     5641 gccaccaaca gaggatacag tgttaaatcc accagtacca tcagtaagtg atacagtgga
     5701 aaaaactcca gaactatcaa gagatgatac aataataaaa tcaccaaaaa caggtgatga
     5761 aactcagcta atgtcatatg tatttattag tgtcattgca atttgtggat tagcgtatca
     5821 atgtaaaata aaaagaaatt aatctagatt attattaagt ttaaaataaa aagtctcaaa
     5881 ttgatttata agtcattttg gggctttttt atgtaaatat aatatgcaaa tatagtttgt
     5941 ttaaaaatat atattttaat tatattctta aatttatgat tggatacagg cattcattaa
     6001 ttgtttgatg aatagaaaaa tgttttgaag gtgaaaatag tattgaaaaa ataatatact
     6061 aagggtataa ttcatttgat ttcatttgta taaatttaat tataaatttt aaagtgatta
     6121 tataaattta aatgctaatt acttaataaa ttttaataat aattatttaa taaataatta
     6181 ttataatatg ctagaataaa tatatataaa taagttttga tgaggtacta ctataaaggg
     6241 gggactttat gttttcgata ccagaagtaa aaaaagtaga agaggtaatg gatacaaaat
     6301 ttacaactat agacgaagac acaagaattg aagatgcaat aaaagaaatg ataaaaagca
     6361 acacaaaaac tctaatggta atagattcat ctgaccaatt aaaaggcatt atatccatga
     6421 cggatataca taatctttat gagatgcata aaaagtatga aggtcaacca gtaaaactta
     6481 taatgaagaa ggatgtaata tatgtcaacg aaggtttaac cttagatgaa tgtagagata
     6541 taatgatatt aaaaaatata gggattcttc ctgttcttag agataataaa attattggag
     6601 ttctaaaaca agagcacatt agggactatc tatatatgca tttagaagat tatggattaa
     6661 ctttgaagta tattataggt caaattaaag aaggaatttg tgctataaat aacgaaggtg
     6721 ttgttatctt gtggaataag tttatggagg aaaggtatga tataaagtct gaagatatag
     6781 ttggtcgacc gatgaatgaa tttttggaaa atactatttc agaaaaagta ctaaatagta
     6841 aagttggaat gagtgacttg tattttacag ataaaaaaga aaatatgtat gcattagttc
     6901 atgcaaatcc tatattttat aaagaagaat ttatcggtgt tgtatgtaca gaagttgatg
     6961 ttacagaagc taagatatta gctcttgaac ttgaaaaagt aaatgataca ttaaaatatc
     7021 taaagaacga agtcaaaaat ttatctaaag gtagttttga taagatttta ggtaaaagtt
     7081 ataaattgga aaaatcgaaa gcgattgcaa aacaagtggc tagaacaaat agtagcatat
     7141 ttatttgggg cgaaagtggt acaggtaaag aagtatttgc aagagctatt catgattata
     7201 gtgagagaaa aggtcaattt atacctgtta actgtagtgc cataccaaat gagttatttg
     7261 aaagtgagtt ttttgggtat gaatcaggtg cgtttacagg agcaagtaaa aagggtcgaa
     7321 taggtatatt tgaacttgca aaagatggaa ctgtattttt agatgaaatt gccgatttac
     7381 ctcttagtat gcaagcaaag ttgcttagag tattacagga aaaagaaata agaagagttg
     7441 gtggagatac cacaataaaa ataaatccta gaataatatc agctaccaat aaagatttga
     7501 gtaaaatggt aaaggcagaa aaatttagag aagatttata ctatagatta aatgttgttg
     7561 aaataaagat tcctccactt agagagagaa aagaagacat aggattattg gttcatagct
     7621 ttttagagga gatatgtaaa caaaataata agcctgttct tacaattagc aaagatgtaa
     7681 tagatatatt tcagaattat agatggaaag gtaatattag agaattaaaa aatacgattg
     7741 aaaatattgt tgtattgtca caaaattcta aaatagatgt ggatgatgta ccaagttata
     7801 tgatggattc tacaaataat agtactgaag aagaagagta tcctttagat ttaacgaagg
     7861 caactcaaaa aattgaaata aagaatataa ccaaggcact taaaatgtct aatggaaata
     7921 aagctaaagc agctaagata ttaaatatac ccagaacaac tttgtattat aagatagacc
     7981 aatacaaaat agatgtgtct aaaatatgac acatgactaa attttgacaa aatgataaac
     8041 tttaatttaa aacctagtaa agccaacgct tatataagtg ttgtgtatat agatgtgtta
     8101 aaaaaaatga aaaagtgttg attaattgac actttttttg tttgagaaaa tgtttacaaa
     8161 tacaaaaaat aacatatttt aaataaatat ttatataata tttggctaaa aatagcgata
     8221 gatgaagaat ctattataga aaaaatcttt ttgttaataa gttggcatag gaattgctta
     8281 taatataaag tgactttaag tgagaaaaaa ataatattaa tcataacaaa ttatgtaggt
     8341 tttaatttac ttaatttctc agttaagggg ttacctgttg aggaattgag atttaaaata
     8401 ataaataaga aaataatgta aggaggtatt ttatactatg tcaataactt tagaaacagc
     8461 tcaagcccat gcaaatgacc cagcagtttg ttgttgtaga tttgaagcgg gaacaattat
     8521 agcgccagaa aacttagaag atccagcaat atttgcagac ttagaggatt ctggattatt
     8581 aacaatacca gaaaatggat taactatagg tcaagtacta ggagctaagt taaaagaaac
     8641 tttagatgca ctttctccaa tgactacaga taacgtagaa ggatacaaag caggagaggc
     8701 taaagaagaa gtagtagaag aaacagtaga agaagcagct ccagtatcag aagcagcagt
     8761 agttccagta agcacaggag ttgcaggtga aacagttaaa atacacatag gtgaaggtaa
     8821 gaacataagc ttagagatac ctttatcagt agctggtcaa gcaggagttg ctgctccagt
     8881 agcaaacgtt gctgctccag tggcaagtgc agcagcagaa gtagctccaa aagttgaaga
     8941 aaagaaactt ttaagaagct taactaaaaa acactttaaa atagataaag ttgaatttgc
     9001 tgatgaaact aaaatagaag gaactacttt atacatcaga aacgcagaag aaatatgtaa
     9061 agaagctaat gaaactcaag agttagttgt agatatgaag ttagaaataa taactcctga
     9121 taaatatgaa acttacagtg aagctgtatt agatatacaa ccaatcgcta ctaaagaaga
     9181 aggcgaatta ggttcaggta taactagagt tatagatgga gctgtaatgg tattaactgg
     9241 tacagatgaa gatggagttc aaataggtga atttggttct tcagaaggtg agttaaatac
     9301 tactataatg tggggtagac caggtgctgc tgacaaaggt gaaatattca tcaaaggtca
     9361 agtaacaata aaagcaggaa ctaacatgga aagaccagga cctttagctg ctcaccgtgc
     9421 atttgactat gtaactcaag aaataagaga agcattaaag aaagttgaca actctttagt
     9481 agttgatgaa gaagtaatag agcaatacag aagagaaggt aaaaagaaag ttgttgttat
     9541 aaaagaaata atgggacaag gtgcaatgca tgataaccta atattaccag ttgagccagt
     9601 tggtacatta ggagctcaac caaacgttga cttaggaaac atgccagttg tattatctcc
     9661 acttgaagta ttagatggtg gtatccatgc attaacttgt ataggacctg catcaaaaga
     9721 aatgtcaaga cattactgga gagagccatt agtaataaga gctatggaag acgaagaaat
     9781 agatttagta ggtgttgtat ttgttggttc tccacaagta aatgctgaga aattctatgt
     9841 atctaagaga ttaggtatgt tagttgaagc tatggaagtt gatggagctg tagtaactac
     9901 tgaaggtttc ggaaacaacc atatagattt cgcatctcac atagagcaaa taggtatgag
     9961 aggtatacca gtagttggtg taagtttctc agctgttcaa ggtgctctag ttgttggtaa
    10021 taaatacatg actcacatgg tagacaacaa taagtctaag caaggtatag agaatgaaat
    10081 attatctaac aacactttag ctccagaaga tgctgttaga ataatggcta tgcttaaaaa
    10141 tgctatagaa ggtgtagaag ttaaagctcc tgaaagaaaa tggaatccaa atgttaaatt
    10201 aaataacata gaagctatag aaaaagttac aggagaaaaa atagtattag aagagaatga
    10261 gcaatctcta ccaatgagta agaagagaag agaaatatac gaaaaagacg aaaactaaaa
    10321 tttagatact aactaagatt tagacaataa cataaattta gatccaagtt taaaacagta
    10381 tagagaggta gaattataat ggataggtta aaatatatct catcagagac tttttatgaa
    10441 ggtattatag ttgacatcaa aggcggtgga gtgactatag acctaaaagg tagacttgga
    10501 caatttaaaa tacctaatag aatgcttata actgactacg aacttaagat aggtcaagaa
    10561 gttggattca tgctaagcta tcctgaggta ttaagtccag agcctaatca agagtatgta
    10621 gaaaatatta ggagagaaaa agaaaaacaa gcagaaatgc aatcaaaaca agaagaaaaa
    10681 taataaataa tcgtttgaaa atagaaggga gaggaaatat atgagcctta caacagtaca
    10741 aggacttcaa tctgaaatat tcgttccaat aacacctcct ccagtttgga ctcctgtaac
    10801 taaagaatta aaagatatga ctatagcttt agctacagca gctggtgttc atttaaaatc
    10861 tgacaagaga tttaatctag caggagatac tacatttaga gctataccta atacagctac
    10921 agttgatgaa atgatggtat cacatggtgg atatgacaat ggagatgtaa acaaagatat
    10981 aaactgtatg ttccctatag atagattaca tgaattagct gcagaaggat ttatcaaagg
    11041 agtagctcct atgcactatg cattcatggg tggtggagga aaccaacatg tcttcactga
    11101 agaaactggt cctgctatcg ctgctaaact taaggaagag ggagtagacg gtgtagttat
    11161 gacagctggc tgaggtactt gccatagaac tgccgtgatc gtgcagagag caatagagga
    11221 agctggaata cctacaataa taatagcagc tcttcctcca gtagttagac aaaacggaac
    11281 tcctagagca gttgctccat tagttccaat gggtgctaat gctggtgaac cacataatat
    11341 agaaatgcaa acacatatat taagagatac attagagcaa ttagttgcaa taccatctgc
    11401 tggtaagata gttccattac catacgaata taaagctcac gtttaaaatg taatttatat
    11461 ttgaaaatat agaacccttc tcctacacgg ttaggagagg ggttttaaga acaaaggtgg
    11521 atattatgga agaaaaaata atgagaagac ttgttataaa accgtttcat atgaatgaag
    11581 ttaatttcgg ttcaaaaact tctataaaga aagatgtact tacaatagat ttatcaagta
    11641 tagatgaaat aaaagaaaga gaagagttaa taacagacat aaaagtagac attataaaac
    11701 ctggagatta tgacagagaa attaatacta ttatggacat tataccaata tcaacaaaag
    11761 ttttaggcag actgggagag ggtattacac atactttaac gggtgtatat gtaatgttaa
    11821 ctggtgcaga tgaagatgga aatcaaatgc atgaatttgg ttcttcagaa ggaattttga
    11881 aagaccaaat ggtatttggg agatatggaa ctccatcagt tgaagattat ataatccatg
    11941 tagatgtaac tttaaaggga ggtttacctt ttgaaagaac acttccactt gcagcattca
    12001 gagcatgtga tgattttata caagaaataa gagcgtcttt gaaaatggaa gacggaagaa
    12061 atgctactca agttcgtgaa tattttgaca aaattcgacc aaatgcaaaa aaggttgtca
    12121 tagtaaaaca aatagcaggt caaggtgcta tgtatgacaa tcagttattc tcaaaggaac
    12181 ctagtggatt tgaaggagga acatcaatta tagatatggg aaatgtacct atgattatat
    12241 ctcctaatga atacagagat ggtgccctta gagctatgac ttaagagagg tggatattat
    12301 gggaatagga ccttcaacaa aagaaacatc tcttcatcac tttagagacc cacttttaga
    12361 catagttgaa agtgataagg atgtagattt acttggagta atagtagtag gaactccaga
    12421 tggaaatgaa aataaaacct ttgtgggaca aagaacggcc gcttggctgg aagccatgag
    12481 agtagatgga gctatagttt catcagatgg ttggggaaac tcacatgttg actacgctaa
    12541 cacatttgag gaaataggaa aaagagatat acctgtagtt ggagttacat ttaatgggac
    12601 tcaagctaag tttgttgtaa gcaatcaata tatggatacc atagttgata tgaacaaatc
    12661 aaaagaaggt attgagacag aagtagttgg agaaaacaac acaaatgaaa tagatgctaa
    12721 aaaagctttg gcatttttaa aacttaaaat gagaaaacat ggataaaaaa tttaagtaag
    12781 ggaatgagtt ttgtgggaat ttctacatca atgaaaaaca catcacttca tatatttaga
    12841 gacccgttac tagatttgat agataatgat tctgacatag acttgttagg agtggttgta
    12901 gttgggacat cagagcacaa tgaatggaaa acatttttag caactagact tggaagatgg
    12961 attgaatcat tgcgacctga tggagtgatt ataactttag atatggcagg aaaccagcat
    13021 attgatttta caaatgctat agcagagttt gtaaaatcag atatacctac agtaggtctt
    13081 actattatgg gagcagatgg attagtaatt accaatccat atttggataa agctaccata
    13141 attgattata aaaagacaac aggctacata gaaacagaag tagttggaga caatcacatg
    13201 gatgaagttg atgttaaaaa agcattggct ttcctaaaat taaaaatgcg taaagacgca
    13261 aaataaacct aagggggaat ttgatatgaa atttagcaga agtatacaag ctatagactc
    13321 tcatacagca ggtgaagcga ctagaatagt tgtaggtgga atacctaaca taaaaggaaa
    13381 ttctatgcct gagaaaaaag aatatttaga ggaaaatcta gattatttaa gaactgctat
    13441 aatgttagag ccaagaggac ataatgatat gtttggttct gtaatgactc aaccttgttg
    13501 cccagatgct gactttggaa taatcttcat ggatggtggc ggatacctta acatgtgtgg
    13561 ccatggtaca ataggagcta tgacagcagc tatagaaaca ggtgtagttc cagcagtaga
    13621 gcctgtaact catgtagtta tggaagctcc agcaggaata ataagaggag acgttacagt
    13681 tgtagatgga aaagctaaag aagtatcatt cttaaacgta ccagctttct tatataaaga
    13741 aggtgttgaa gttgacttac caggtgttgg aactgttaaa tttgacatat catttggtgg
    13801 aagcttcttt gctataatac atgcaagtca attaggttta aaaatcgaac ctcaaaatgc
    13861 tggtaaatta actgaactag ctatgaaact tagagatata ataaatgaga aaatagaaat
    13921 acaacatcca actttagctc atataaaaac tgtagactta gttgaaatat atgatgagcc
    13981 aactcatcca gaagctactt acaaaaatgt agttatattt ggtcaaggtc aagttgacag
    14041 atctccatgt ggaactggaa caagtgctaa attagctact ttacatgcta aaggtgaatt
    14101 aaaagtagga gaaaaattcg tatatgaaag tatattagga actttattca aaggtgaaat
    14161 agttgaagaa actaaagttg cagatttcaa tgctgtagta cctaaaataa ctggttctgc
    14221 ttatataact ggatttaatc attttgtaat agatgaagaa gacccactta aacatggatt
    14281 tattcttaaa taagtaataa ataaattagg aaattaaaat agaatagtag ccacttgtcg
    14341 gttgctattc tatagctttt aggctataag attcagaata tatgaaaaaa taaaaaaata
    14401 ggaggatgga ttatggtagc aatcttaaag gcattatatg tagtattcgc agcaatcttt
    14461 gcagttgtgt ttggtaaaga cgtagctaaa gcaaacaaag aggggaaact agaagaacaa
    14521 agtactccaa aagttgctgt aacaggattt ataacagact tctttgatac attaggaata
    14581 gggtcatttg ctccaacaat agcaatgtca aaggctttaa agttaaatat accagataaa
    14641 aaaatgcctg gtacattaaa cgtagcacat actattccag ttgttacaga agcattcata
    14701 tttacatcaa ttatcccagt agatggtgtt actttagtat ctatggttgt ggcagcagca
    14761 gttggttcat atataggtgc aggaataata gctaagatgg acgagagaaa aatacaactt
    14821 gttatgggtg taactttagc attaacagca atattaatgt tattaggtca tccttggatt
    14881 aacgtattac caggtggagg aaatgaatta ggtcttactg gtgctaaatt agtaataggt
    14941 gtaataggta actttatact aggagcttta atgacagctg gtataggtct atatgctcca
    15001 ggtatggcaa tggtttattt cttaggaatg tctgcaaaag ttgcattccc aatagttatg
    15061 ggttcttgtg cattattaat gccagttgca agtatgaaat tcataaaaga agatgcatat
    15121 actaaaaaag catcagttat aatagctata tgtggtttaa taggagtttt cgtagctgca
    15181 tatatagtaa aaactctacc aatggatata ttaaaagcat tagtaatagt agttatagct
    15241 tatacttctg ctacaatgat aatggctgca aataaaaaca aaaagactgc ttagttaaag
    15301 actacttagt gtttattaaa tattacagct aataataaag attataaact gctttaaaat
    15361 attgaattaa tattttaggg cagtttttta tttactaaat taatataaag caattaacat
    15421 gccaattatg taatatatat agtgatttag tagatttaat ctaaaaaacg ttg
//
LOCUS       DAELNY010000081        15419 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.081, whole genome shotgun sequence.
ACCESSION   DAELNY010000081 DAELNY010000000
VERSION     DAELNY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.081"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(375..1265)
                     /gene="metA"
                     /locus_tag="KPE55_002144"
     CDS             complement(375..1265)
                     /gene="metA"
                     /locus_tag="KPE55_002144"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG0493207.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1295..2560)
                     /locus_tag="KPE55_002145"
     CDS             complement(1295..2560)
                     /locus_tag="KPE55_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG0493208.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2670..2772)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2826..5483)
                     /locus_tag="KPE55_002146"
     CDS             complement(2826..5483)
                     /locus_tag="KPE55_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0493209.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGVFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5611..6354)
                     /gene="yaaA"
                     /locus_tag="KPE55_002147"
     CDS             complement(5611..6354)
                     /gene="yaaA"
                     /locus_tag="KPE55_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG0493210.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6364..6828)
                     /gene="bcp"
                     /locus_tag="KPE55_002148"
     CDS             complement(6364..6828)
                     /gene="bcp"
                     /locus_tag="KPE55_002148"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG0493211.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(7180..8877)
                     /gene="ade"
                     /locus_tag="KPE55_002149"
     CDS             complement(7180..8877)
                     /gene="ade"
                     /locus_tag="KPE55_002149"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG0493212.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(9183..10337)
                     /locus_tag="KPE55_002150"
     CDS             complement(9183..10337)
                     /locus_tag="KPE55_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG0493213.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(10582..11421)
                     /locus_tag="KPE55_002151"
     CDS             complement(10582..11421)
                     /locus_tag="KPE55_002151"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG0493214.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(11434..12030)
                     /locus_tag="KPE55_002152"
     CDS             complement(11434..12030)
                     /locus_tag="KPE55_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0493215.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(12081..12731)
                     /locus_tag="KPE55_002153"
     CDS             complement(12081..12731)
                     /locus_tag="KPE55_002153"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG0493216.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(12777..14030)
                     /locus_tag="KPE55_002154"
     CDS             complement(12777..14030)
                     /locus_tag="KPE55_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0493217.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(14054..14695)
                     /locus_tag="KPE55_002155"
     CDS             complement(14054..14695)
                     /locus_tag="KPE55_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0493218.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(14897..15310)
                     /locus_tag="KPE55_002156"
     CDS             complement(14897..15310)
                     /locus_tag="KPE55_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG0493219.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
BASE COUNT         4840 a         2758 c         1639 g         6182 t
ORIGIN      
        1 tgtcaattac aatattgtaa cttcaattgg aattttgtta tcttattgat atttaatttt
       61 atacaggata tttttaataa aatttaataa aaaatctata tatatatttt acactgattt
      121 ctcccttttt aatctctttt aaaagttaaa atacaaattt attgatacta taaaggttta
      181 gtctcccatt atcgcaccat atataaaatt aaagccacct ctatctacat acacctaaag
      241 tgattttctt aaaatataaa actactatta taaggtcaaa tttaaaaaaa ataaggtata
      301 aagtatcatt aagattaact ttatacctat aaatttctat atttttttag ttaacattta
      361 tatactctta aaatttattg tatcaaatag ttttttatcc aatttctaaa caacaattct
      421 gaatgttttt tccattttac agttggctct tctgaagggt catcattaat atagtaattt
      481 tgaggtatag atattttatc tcctttattt atatccctct tatattcttt atcaagagtg
      541 tatttgtcat actcgctatg acccattacg aacacatctc ttgaatttgt aattatatag
      601 ggaccagctt ctttagaatg actcacaatg ctcaactctt tattctcttt caaagcttca
      661 atatttacag tagtatgtct tgaatgtgga gcaaaaaatt cattttcaaa tccatctact
      721 attttactat ctttatctac cttatgctta aatactccaa aacatttttg acttagtgga
      781 agttttttta cattgtagta tttgtaaaga cttgcttgag ctgcccaaca tatatataat
      841 gtagatttag tttttctctt agaataatcc ataatttctt ctagttcatt ccaataatca
      901 acttcttcaa actccatttg ttcaactgga gctcctgtta ttataaagcc atcaaaatac
      961 tctcctttgg cttcatcaaa tgttttataa aattctttaa catattcatt acaacttctt
     1021 ttactttctc ttgttgaaat tttaataaat tccacagaca cgttgaatcc tgttttatct
     1081 atccttctta gtaaatctaa ttctgtatct atctttatag gcattagatt tagtatagct
     1141 atcttagtgt ctatgttttc ttcatacttt aattcctttt taaaatcttc tttataaatt
     1201 acatctattc cttcatctaa taatttatta ataacaggta atccctttgg tagtattaaa
     1261 gccatatctt ttcacttctc tttctcttag aatattagaa atttaaagct tgatttaaat
     1321 cagctattaa atcctctaca ttttctattc caacagataa tcttattagt gaaggcaata
     1381 ctccagatgc aattaattgt tcttctgtta attgtctatg agtagttgaa gctggatgta
     1441 taacacaagt tcttacatct gaaacatgtg taaccaaaga tgctatctgt aatttttcca
     1501 caaatttttt ggcattttct aacccacctc ttactccaaa tgataaaaca ccactacatc
     1561 ctcttgataa atactttttg gcattctcat aatacttgtt atcttcaagt cctgggtaat
     1621 taatccaatc tacattttca tgtttttcta aaaatctagc tattttaagt gcattttcac
     1681 tatgtctctc cattctaaga tgtagtgttt caacatttaa attagtaaga tacgcattga
     1741 atgggcttaa acaatttcca tagtctctaa gcaactgaac tcttgcttta gttgcatatg
     1801 cggcatttcc aaatttttga gtatagctta taccatgata tgttgggtct ggctcaacta
     1861 attctggaaa ttttccatta tcccagttaa agtttccacc atccactata attcctccaa
     1921 cacttgaagc atgcccatct aaatattttg tggtagaatg agtaactata tttgctccat
     1981 actttaaagg gttacaaagc actggagatg ctaatgaatt atcaacaata aatggaacat
     2041 taattctctt agctacattt gctatttttt caaaatctat gacttcaaga gttggatttg
     2101 caagtgtttc tgcaaacaca accttagtat tttcctttgc aagttctact atctcatctt
     2161 cacttgaatc taagtcaaac gatattaaat ctataccaaa ttttttaaga ctaggtccta
     2221 gtaaattgaa tgttcctcca tatacttttg aagaacaaag tatactatct cctgatttac
     2281 atatatttaa aactgccaac atatttgcag attgccctga tgatacagct acagaagata
     2341 ctccaccctc tagtaaactt attttttctt caaaagcttg aatagtagga ttgcttattc
     2401 ttgaatatat atgtccatca acttttaaat caaataattc agcaagttgg tctatactgc
     2461 tatatttaaa agttgtactt tgatataaag gtagtactct tggttctcca ttacctggtt
     2521 tataatttcc ttgcacacat attgtttctt tattatacat tcttgtatcc cccttagatt
     2581 ttagtaaaaa aaattaaact atttaccaga tgagagttaa aataaataaa gcctgagtat
     2641 ataactcagg ctctgtataa gcttaattat actcatctct cagctttcgc cgcaggattt
     2701 agcaccaatg tatataaata tactggttgc cgggtttcat agggccagtc cctccacctc
     2761 tctggataag atttatttaa tttttctatg ttttatatct tatatatttt tcctaatttt
     2821 gtcaactatt tttttaattt ttttctttta ataattttcc caacctctac ttgaattaca
     2881 gacattatgg ctaaaacaat cgttataagc cactctaagt tatttaaaac tgttaagtta
     2941 aaagcatttc taaaaacagg tacaaacagt attactgcta ttaaaaatgc agaagttaaa
     3001 aatgagatta aaagaaatgg atttttacca ttatttctat taaacattga atctgtattt
     3061 gaacgctgac taaatgctcg taacatctgt gaaaatgcta atacacaaaa tgccattgtt
     3121 tgacctacag aatgattttc tatattaaga ccaattttat atgctaaaat agtcattatt
     3181 gccacaaata caccttgggt aacaactctt tgtataagat ttttttcaaa caaagtggtt
     3241 gatttcactg gctggtgctt cattatattt ctgcttggtg ggtcaactcc aagagcaagg
     3301 gctggaagcg tagcagttgc aaggtttacc catagaatat gaacagcaag aagcggagta
     3361 tcccaattta ataatattgc tacaaacaac gtaagtatct ctgctatatt accaacaagc
     3421 aaaaattgta taactttttg tatgtttcta tatacacgtc taccttcttt tattgcataa
     3481 gcaattgttg taaaactatc gtccattaaa atcatgtcag aagaatcttt tgcaacatct
     3541 gtaccactta tacccatggc tataccaata tcagcagact ttaaggcagg agagtcattt
     3601 acaccatccc cagtcattgc tggtacttct ccaattcttt ttagagattg tataattcta
     3661 agtttatcaa atggggatac acgagcaaat actgtagtat ttttaacagc ttcatctagt
     3721 tcatcatcac tcaaattatc aagttcatca ccagatatca cagtattttc ttcactccat
     3781 attcctaact catgagcgat tgttgtggca gtaactttat ggtcacctgt tatcattatg
     3841 gttcttatac ctgcttcttt acaagtactt actgcatcaa ttacctcagt tcttggtggg
     3901 tcaatcatac caaccatacc tataaatgtt aaatcatatt ctatattttc actatcttct
     3961 tttggtatag atgaaatctc acgcttagca aaaccaagta cacgcaatgc atctttagac
     4021 atgttaagac ataattcgtg tatattcttt ttatccactt cagtaatgtt tctttcacct
     4081 tttgaagtta atattttagt acataggtca agaatttcgt ctactgctcc tttagtataa
     4141 gcaataatct tttcatctat attatgaaca gttgtcatac attttctatc tgaatcaaaa
     4201 ggctgttcaa atacacgtgg atattcttct tcaagagctt cttgatctat tccaaatttt
     4261 ttggccataa aaattaatgc gccctctgtt gggtcaccta atatttcatc tgttttatct
     4321 ggattaatac atgcatcatt acaaagtgca ccagcataaa caatctcttt atacactaat
     4381 ggatgttttt cagatgcagt atctattgat gtcacttttc cagattcaaa atctccattg
     4441 acagcaatat gtgtaacagt cattttattt aaggttaatg taccagtttt atcacaacat
     4501 attactgtag ctcctcctaa tgtttcaaca gcaggtaatt ttctaattaa agcatttctt
     4561 ttagccatac gctgtacgcc taaagccatt acaatagtag ctgttgcagg taatccctct
     4621 ggaataattg atatggcaag ggaaattgca atcataaaca ttggcaagag aggtctctga
     4681 taaaatgcac ctattgcaaa tataacaacg catacaataa tacctatgac acttaatgtc
     4741 tttccaacag tattaagttt acgttttata ggagtatcaa aatcatcttg tgaatctaaa
     4801 agatgtgcta tgttacccac ttctgtatcc ataccagttg ctactacaac acctacacct
     4861 ctaccatatg tgactattga agatgtatac gccatattgc tacggtctcc taatacacat
     4921 tctttaggta agattgtatc tgcatctttt tctgatggaa cagattctcc tgttaaagaa
     4981 gcctcttgaa tttttaaact tgatgtttct attaaacgaa ggtctgctgg aaccatagct
     5041 ccatcttcta aaaaaacaat atctcctact acaagattac ttgctggaat aacattttct
     5101 tcaccatcac gcataacacg agctgtaggt gaattcatat tttttaatgc ttctaatgat
     5161 gattctgctt ttttctcttg aacaattcca ataactgcat ttactgttac aattattagt
     5221 attacaaaag cttctgtaaa ttcgccaaat atcaaagata atattgctgc tcctattagt
     5281 atcataacca tagcatctgt aatttgactc caaagcattt gtaaagttgt tctaggtggt
     5341 ttttttctaa gttcatttaa gccattctct ttaagccgtt tttccgcctc ggtatcggtg
     5401 agcccctcct ctgatgaatc tagactttct aaaatttcat gcgttgctct tgaatgccaa
     5461 ggcatctgtt tgtgattttc cattttggaa gaccactcct ttcttttttt aatttaagaa
     5521 taattatatt ttaaaatcaa gttcaattca atacacacta agtatatttt acctaaattt
     5581 aacaatgcca tttaatattt aacaaaactt ctactctttt acaaatatgt attcactatt
     5641 gttagataag ttttctttat acttataacc ttctaaatca aactttttca gttcttcaat
     5701 atctcttatc ctatttttta caatgtattt tgtcatcata cctctagctt tctttgcttg
     5761 tgtagattcc actttcaata tgccattttt atttacttta aaagtacatg taatatatgt
     5821 atcactatct ttaataaatt tttctatgga tttgctgtat tcatttgatg aaagattaac
     5881 gatagctgtt ttttctttga ttagttcttt atatatacga tttccccaat attcgtataa
     5941 attcttaaac tcacctactt gtaattttgt ctgcatttct aatctatatt catatatact
     6001 atcatatggt tttacaacac catacaatcc acttatgatt cgaatacagc tatttgcaaa
     6061 ctcttcatct ttccttgtaa aattttctgc ttctatattt ttatattgta ttccatcata
     6121 agttaagatt gcagggtttc ctaatctatc aaatttaatg ctttcaaatc tatttttatt
     6181 taatacagct aatttttcat taattttcat tttattttta aaatcttcta taccaaatgt
     6241 ctttatattt tccactattt cttttgtgtt atctataaag caaggcaaac tcaaatctct
     6301 atcaaatact tctatattct tcatattttt agctggtgat aacattataa tcataaattc
     6361 ttattactcc tgtttttcca aatattcaag aatctcttgt gcatttgtgt ctggttttgc
     6421 tttttcaaag actttttcaa taattccatt ttcatctata atatatgtcg cacgtacaac
     6481 tcccaatgca gtttttccat acattttttt ctctttccat acatcaaaag cttttatagc
     6541 aactaagtca gggtcagaaa gtaaaataaa aggaagttca tgcttttctg caaatttttg
     6601 atgtgatttt atactatctt tacttatccc aataacttgt atatcttcct ttttaaatcc
     6661 atcatatgca tttctaaaag cacaagcttg acgtgtacat cctggtgtat tatctttagg
     6721 ataaaaatat actactactt ttttgccttt aaaatctgac atacttacct tatttccatc
     6781 tttatcttct agtgtaaatt ctggtgcttt tgttcctata ctcaacataa atatctctcc
     6841 ttttgttttt attaataatt ttatactatg cttatattat taccctataa atctatatat
     6901 aaataaaata ttttattttt tatgtataaa gtttcataaa tatataattt tcatgtattt
     6961 atatgttaaa atagactgaa aatagtttta ttaagatatc tatgcttaaa acaatgttaa
     7021 atgatagatt aattaaaata aggatatcta aaaataaaat ttttagttca tgagatgctc
     7081 ttttttgtat gaaatcaaat atattttcat catttcaaca ataaaaagag acttatctct
     7141 attttgagaa agcctcattt attattactt ttatactatc tatataaatt taaattcctc
     7201 aacatcaaat agacctaaat ctgtaagtct tatttgtgga acaactggaa gtgccataaa
     7261 agaaagtgta ataaaagggt ctacatactc tggaacttcc atttttcttg ctatctccaa
     7321 catattatca gtaatttctt gtacttcttc agctgtaaaa gtactaatta atccagctac
     7381 ttgaagactt aggtgtgcaa gaacttttcc atttgataca attacataac caccttgtat
     7441 ttcctttaag taatttatag ctactagaat atcatcatca ttatcccctg caactataat
     7501 attatgagaa tcatgggcca cagtagtagc aattgctcca ttttttatgc caaatccttt
     7561 tagtggagca attgctacat ttccagtcat tctatgtctc tcaacaacac aaagttttga
     7621 atactcttta tttggaacaa aatatccatc tgcacatggt aaagattcat ataccttatt
     7681 tgtcaaaatt tgataaggta caatctccat tacataattt ttttcactta cttttaactg
     7741 tattttttct ttattaatat atttaaactt gactgtatta agcaattctt tatctttaat
     7801 ctcataattg taattagaaa acatctcttc gtttaccaaa ttaccatctt tatatacact
     7861 atccacttcc attttgtcta aatcattcaa tacaacaata tctgccttat aacccgcacc
     7921 tattgctcca attttcctta acccatatgc ttttgcagaa ttataagttg caacttttat
     7981 agccctaaca ggttccatac ctaaatcaat cgcacatttt atattccatc ttatgtgacc
     8041 ttgtttttct atatcatcta agtgcttatc atctgtgcaa aacataaatt cttcaattgg
     8101 caagttatgc tttagcatac cacttactat agactttaaa ttttttgctg cgcttccttc
     8161 tcttattaaa atttttaatc ctgctctcaa tttttcatat acttcatcaa aagttataca
     8221 ttcatggtca ttttctattc ctgcacatac atatgtctga agtgatttac catttatatt
     8281 aggtgcatgt ccatctacaa ctttattttt aaaaagctct agtttatcca agatttcatt
     8341 ttcagaattt ataacatcat taaaacacat tacctctcct aaacctaaaa ttctaggatt
     8401 atttacataa ctctccatgt cttttgcact aaatttacca actccattag tttcaaaact
     8461 tgttgcaggt acggatgaag gcaccataat atatacatta agtggtatat cttttgtcga
     8521 ttctaataaa aaatctattg cactaactcc ttttacattg accaactcat gtgggtcggc
     8581 tatgatagtt gtcgtacctg attttaataa ttttttcgat aattcaattg gcattaccat
     8641 tgtagattca atatgcatat gtgcatcaat aaagccaggt gatatatatt tgttactaca
     8701 atcaatttct acattgccct tgtaatcgcc aattccaaca atagtgtctc ctactattgc
     8761 tacatctcct gttattaact cttctgtaaa tacatttatg attgtcccat tttttagtac
     8821 aatgtcagct ttttctcttc cacttgaaac atcacactgt ctttttaaat tacccatttt
     8881 tacaactcct cctaagtttt ttatatcaat atactgactg tattacgtat tatagtttaa
     8941 tttattaata tatattatat atgagttttg atagaaagta tcacttgaaa tttgttccat
     9001 aattccttta attaaataga gagaacatga ggaataatat tatctcccat gttctcatta
     9061 atatttaagc aataatttaa ttcatattga attttttatt tttatattta atcttcacta
     9121 ttcttataaa taaatttatg tgctttatta tttttttaat acttctttat tcataattat
     9181 atttaaccta aaaatgcata aactatcata cctacaagta ttgctggtat agtagtaata
     9241 atcatagccc ataaagaaga ttttttatcc attgctaaaa gtgggaaaag tgcatctcca
     9301 tcttgagaaa tagagttggc tatcactgca ccaagtggta tagttccctt caaataaaat
     9361 gacataagca caatttgtat cccacaacct ggaataagtc caagacctgc acctataaat
     9421 acacttatta cacctgttga taatactaat ttaactaagt attcatctcc acctaaaatt
     9481 aatattatca aatcgtaaat aagatatgca ataaaaatcc atgtaattac aaatgatatt
     9541 tctccaacag aatggataag catttctcgc aaagaaatct ttttattttc ttcctctttt
     9601 tgattagaat ttttaaatac ctttttaaaa caccacatat aaattattga aaataatatt
     9661 ccaatcactg ctattacttc ttccaatgaa tgtaacttct caattatagg aagattaagt
     9721 ccagagtgag caagtgacat aaatataaaa ccgattacta taattcctat ataaatttta
     9781 tatccctttc catgtgttat tataaatgca agtctatttg tatttccatg agatttagct
     9841 agagtgtcta aaattatttc atcatgatta tgttgttctt tattttcttt agggttattt
     9901 ttacttatat catatttatc tgattttcta cggtttttta accctaattt ttcttctagt
     9961 ttaaagtaat ccactaattg tccagttatt attcctgtaa ttgtactaac tatagtgaca
    10021 aaaaaataca ttttaatatt tgatgaaata agtacaaatg ctgcatctcc catactagct
    10081 attaaacttg caattattgc tccaaagctt aattttccat ttatatacaa tggcattatt
    10141 gccaaacttc cgccacaacc tggtatagct ccaattaaag cacctattaa gggttgaaat
    10201 tttctgtttt ttgatatgat attagtaaaa tttccactag ttttataatt aatatatcca
    10261 aataacaaaa taaaaaatcc aatcatagaa cctacatgta aaaacgcctc ttcagaagcg
    10321 gatatcaaca tttccattaa tttacctcct agactaattt tgcaacaatt atattatatt
    10381 ctattttcta ttgataatca atatcaatat ttcttttgaa tttctaatat taaaaagtgc
    10441 gcatcttata ctcttagttt ttcatcttaa gatacacact ttttgatatt aggttatcta
    10501 tttaccacat gtaatatgaa atcaaaggtg gatattatga taaattataa caatgtccac
    10561 ctatttttat tacctatcaa attataaatt ttcaaggaat gttacaactt cttctattat
    10621 ccaattaggt gtagatgccc ctgctgtaat acccaaattt ttatattttt taagcgtatt
    10681 tacatccaat tcatctttag tctctattgc aaaagttggt actaagtctt cacaaacctt
    10741 tactaacttt tgagtatttg aactgtgttt acctcctata actatcatac aatcaacttc
    10801 tttagctaac tctttagctg attcttgtct tgtttttgta gctgaacaaa ttgtatcatt
    10861 aaatactatt tcttctaatt tatctgatag tttattaact atacttatat acaactctgg
    10921 atttattgta gtctgtgcaa caagacaata tcttttggag ttatcaaatt ccatattttc
    10981 catttgttct aaggtcttta ttattatagc tgaattttca caccatccat ttatacctat
    11041 aacttctgga tgcttcatat ctccaataac tattatttca tagcctttat tatgatattc
    11101 acttactact gtatgtattt tctttacaaa tggacatgta gtatccacaa tttttaattt
    11161 tttatctttg gcttccttat atatattttc aggaactcca tgagacctta ttatcatatt
    11221 ttcatgattt ggtattgtat caatttcatt cacagtctta agaccttttt cttcatattt
    11281 tgctactgct tgcttgttat gaataagagg acctaaagca tatatgccag aatcattttt
    11341 ttcaacttca tcccatgcca ttttcattgc tctttttact ccaaaacaaa agccagcatt
    11401 ttttgctatt tttatgttca taactttacc cccttatttt gaatatttaa agtaattttg
    11461 ttttatcact tctaaaatct cttgtgatat cctttcatgg tcatcattac tcaatttttc
    11521 cttaaaatgt tcttcaaatg atattggttc tccaatatat acaattactt tactaaaaag
    11581 tttatattta gatattattg atactggaac aactttagat ttacttttta ttgtaaatac
    11641 agaaagacct gccttagcct taccaaaaga atctcctttt attctagtac cctctggaaa
    11701 tattcctaat acataaccgt ctctaactga ccttaaaata gtttttattg tagatacatc
    11761 tggtttttct ctatttatag gcataacatg caattttttt aatataaatc ccaatggttt
    11821 tactttaaac aattctttct ttgcaattgc agctatttct ctattttcta tggcagcagc
    11881 caagaatata gggtcaagat tcgatttatg atttgatgct attactatat ttcctctatc
    11941 tggaatattc tctgcaccta taacttcata tttaaaaaat attttagaaa aacccttaaa
    12001 aatatttatt acaaatctat aaaaattcac gtttatctcc cttcaacact acatttattt
    12061 tcattatgat gcataaaaaa ctaaagattt attttagata caacttcttc tacaacttct
    12121 tctatagttt ttcctgtagt atctatttct agtgcatcat tagcttttac caatggtgca
    12181 aattctctat ttgaatcaat ttcatctcgt cttattatat cctcaattac ttcttctaaa
    12241 gtagtattat atcctttatt acaaagttct ttatagcgtc tatttcctct ttcttctgga
    12301 gtagctacta aaaagaattt gtaatctgca tctgggaaaa catatgaacc tatatctcta
    12361 ccatctagta ttactgaatt tttcattcct atttttcgtt gaacttcaac catcaactgt
    12421 cttacttctt ttatttttgc tacattagaa acattattgc ttacttctat tgttcttatt
    12481 tcttcattta taacttcttt gtccaaatat atattgttat ctttaaaatc tatatccgaa
    12541 ttttcagcta tttgaataac ttctttttca ttattgacat caataccact ttttaaacac
    12601 ttataagtaa ctgctctgta catagcacct gtatctatat aatttatatt tagctttttt
    12661 gctactatct tggctattgt actttttcca gctcctgctg gaccatctac agctataact
    12721 aaattaccca tatatacacc tctatttatt catatttaat tattaaaaaa ttatatttat
    12781 acacttatac ccgcaagata tccagttgaa aatgctattt gtaaattata tccaccagtt
    12841 tctgcatcta tatcaattac ttcacctgta aaaaataagt tcttaactaa tttagattcc
    12901 atagtagaag aattaatttc tttaactgaa ataccaccag aagtaaccat accagtgttt
    12961 atacctgtag tcccattaca agttagcttc atatttttta tatactcaat aattctaatt
    13021 tcatctgcct tagacaaatc acttgccttt ttatcaacta aagataatag agaaaaaatc
    13081 tcttttaaga aattttgagg tagtatttgc ttaagattat ttagaacatt tttatttgga
    13141 aatgctctga ttatactaga gatttcatca gtacttaaat ttggtaagaa atctatattt
    13201 aattctacct tttccttttc aataatcttg ttaatataag aagacattat aagaacacaa
    13261 ggtcctgtta tcccaaaatg ggcaaatagc atgtctccag attttgatat tttccttttc
    13321 ttaatcttac aagatatttc cacattcttc atggatattc cttgtaaatc tttaatccat
    13381 ttttcctcta ttgtaagtgg aactaaagca ggatatagtt tgtttaatgt atgtccatgc
    13441 tttttaagaa tcttatacat tgaaccatct gaacccgtct ttgaataact cactcctcca
    13501 gttgatataa taaccttgtc tcctagtatt ttatctccat tatctaaaat aatccctttt
    13561 attagatact ttgacttgtc cttattagaa tcatctttta gagcaatttc ttcagttata
    13621 atatctataa ctttcttatt atacattatc tttacattgt gattgagtaa atctttattt
    13681 aatacttcta ttacttctat agatttatca tttttagtgt atactttgtg gtcattttct
    13741 tcttcaatct tatattccaa atttctactt tcaaaatacg aaataaggtc tttatttgta
    13801 aatgtataaa aactactata gagaaatttt ttattactga caactttatc aaaaaaatct
    13861 tctatctctc tattattggt aaaattgcat ctaccgccac cagttgccct aagttttctt
    13921 cctaattctc catttctctc aactaatatt acttcattat tgttttttga agctgacaaa
    13981 gctgccatca ttccagaagc tcctccacca actactataa ctttactcat aaatttctcc
    14041 ttataattga ttcctactcc agtgcagaat tatttatctt tacatgattt tttatatgac
    14101 ttgtatcatt catttttact ataacaggcc cataatcttt aaattctact atatttaaag
    14161 cagtattatc ttgcttaatt ctatatatat tttccatacc tgattctaaa aatgacaaca
    14221 acataaccct caatgtaatt gaatgtgtaa caagaattat atttttatta tcatattttt
    14281 catttagttc ctttataaaa gcatctactc tttcctttat aatcttaaga gtttcgcctt
    14341 ctggtatatt tactaagtgt ggttcatttc tccatgttgc atatatatct gaataatctt
    14401 tttgaatttc ttttattaaa aggccttccc aaactccgaa tcccatttct ctaagtgcct
    14461 cagttttttg aacttcaata ttgagcttat ctcctaaaat ttgagctgtt tgcattgctc
    14521 tcccaaggtc gctactaaaa atataatcaa ttgaatactt gccaatatct tcagaaagtt
    14581 cttttgcctg ttctatacct tgaggcgtaa ggtcagaatt accatgacct tgtgtttttc
    14641 ccaatatatt ccaatccgtt tgtccatgtc ttactatata aaaagtattt cccatatgtc
    14701 ctcctaatat atgcctattt tttattaaat ataaattatc acttctcaaa acttaattat
    14761 atcatataat actttacttt gtttctattt tataatctaa tctaacatat tatttacaat
    14821 aaatctcatt tgtatttctg caaattattg tactgttaaa gaatttttct aaatttataa
    14881 acatatttct cttatattat ttatatactt cttcaaatct gtattcttga attgaattga
    14941 catacttatc tgtatctact ttttctaaaa acatatccaa aggtcttgca aatattcctg
    15001 tatcatcata taaggtcttg tataaaacta aatcattagt atcttcttct tttttatgat
    15061 aaaagtttcc atctaaatcc ctataaacat agatatcttc ttctctttca gtatgtctaa
    15121 taactaattt atatttattt ctgttttgga ttaaattttc tttaccatat atttcacata
    15181 tgttttctat atcatcaata gccttgctaa gacccattgt agcataatat ttccctttga
    15241 aatgtttata gatacaagga tattttaact ttctttcact gatacctaag ttattcttta
    15301 attcaagcat tttattataa tctcccatat ctcgtccctt tcttttaaaa agtattttat
    15361 tctgaaataa gttacttgtt ttatatttta tcataattta aaatttatta ataatacaa
//
LOCUS       DAELNY010000082        15350 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.082, whole genome shotgun sequence.
ACCESSION   DAELNY010000082 DAELNY010000000
VERSION     DAELNY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.082"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            86..967
                     /locus_tag="KPE55_002157"
     CDS             86..967
                     /locus_tag="KPE55_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0493220.1"
                     /translation="MNTKQLEYFISVAEKLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(1155..2147)
                     /locus_tag="KPE55_002158"
     CDS             complement(1155..2147)
                     /locus_tag="KPE55_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG0493221.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKALSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(2152..2892)
                     /locus_tag="KPE55_002159"
     CDS             complement(2152..2892)
                     /locus_tag="KPE55_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG0493222.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            complement(2957..4066)
                     /locus_tag="KPE55_002160"
     CDS             complement(2957..4066)
                     /locus_tag="KPE55_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG0493223.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDVVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            complement(4084..5217)
                     /locus_tag="KPE55_002161"
     CDS             complement(4084..5217)
                     /locus_tag="KPE55_002161"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG0493224.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            complement(5219..6313)
                     /locus_tag="KPE55_002162"
     CDS             complement(5219..6313)
                     /locus_tag="KPE55_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0493225.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMDSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(6450..7751)
                     /locus_tag="KPE55_002163"
     CDS             complement(6450..7751)
                     /locus_tag="KPE55_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0493226.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(7976..8677)
                     /locus_tag="KPE55_002164"
     CDS             complement(7976..8677)
                     /locus_tag="KPE55_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0493227.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            complement(8890..9207)
                     /locus_tag="KPE55_002165"
     CDS             complement(8890..9207)
                     /locus_tag="KPE55_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0493228.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            complement(9255..9566)
                     /locus_tag="KPE55_002166"
     CDS             complement(9255..9566)
                     /locus_tag="KPE55_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493229.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIARPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            complement(10008..11180)
                     /locus_tag="KPE55_002167"
     CDS             complement(10008..11180)
                     /locus_tag="KPE55_002167"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0493230.1"
                     /translation="MLKQNTIQIDYTTYGNNYQFKLPLNIDYMIPKDAIFVDGTKIES
                     SANRYTFVWKKTINKNMVRVMNDISNFILKCGIDFGIKIIYRNKIKIYHLKKLLKKLK
                     KISKESNITFVHGKGKKKSIVQKSIAKLNNYIDRLKKYTKDLHIMGELNSYSKTDNDA
                     TFMRMKKDHMKNGQLKPAYNIQFGVDSEYIVWVSSEHKPTDTTTSIPFLKSIEEHTKF
                     KYPKIVADSGYESEENYTFLECNNQLSFIKPANYEISKTRKYKNDIGRIDNMDYDEKK
                     DYYICKNGKKLFANKIINRKSKTGYKSKITCYTCKECCDCQYKNGCIKGNNSKIPLEN
                     RTKNLQVSKLFHEKRKENLKRIISQEGCQLRMNRIIQAEGTFAQVKQNMKFRRFLS"
     gene            complement(11320..12384)
                     /locus_tag="KPE55_002168"
     CDS             complement(11320..12384)
                     /locus_tag="KPE55_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493231.1"
                     /translation="MEKNFLLNKINSIQKEYLELLVKLKDNIDTIDYIYIIDEINIFW
                     YSYRDVIKLYLGNITQKDNAYIFTGATYLDVDDLEHYPFVMMGDIHIVDDSLCKYSKT
                     VLVLSNQEFAKVLKDQIISCIDDNIKILSNYSFAMLILPMNLLYGNDMKIIHEYSNNI
                     FLNFFSRKDLNLDTYFEEFNSIEDIALALNPGIESYIVFDEGEDLTKNLEERFKSFID
                     KGKVPLPAHKSDAFKFWLIIYGFITQALDIILSCKGFNLTPYLRYRVTLQYVLLLGDN
                     VFDIEDLSNMLLKCKVAHVLYAIFDKSEIKNISFVQFYKKLSEYKFSYKVENDIKENI
                     NINQIAEILNKNITLFLNYI"
     gene            complement(12359..13831)
                     /locus_tag="KPE55_002169"
     CDS             complement(12359..13831)
                     /locus_tag="KPE55_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493232.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVKQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEIFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDTVYGIPQHLLLEVIKNGKEFFTK"
     gene            complement(14063..>15350)
                     /locus_tag="KPE55_002170"
     CDS             complement(14063..>15350)
                     /locus_tag="KPE55_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493233.1"
                     /translation="VECEKCGEAISYFIHKEEVDNIIEEEKRKKELFIRTLENVYNKI
                     NKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEIAQEQYRLWQQRAAMMANIK
                     NKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDLVDMQDRGENIFKQLFNNYV
                     RLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGITYSEAVVYELMEKTKLLPI
                     RAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESINKSQNINNQVAYLIFNKQKY
                     ICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYEDITEDEIIIDNIFCVVDERN
                     NELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNLIRAYANTLYKQTKDGFANE
                     KVFFAYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
BASE COUNT         4912 a         2697 c         1522 g         6219 t
ORIGIN      
        1 aaagtaaatt ttcttatact ataataagct ttaattatag ataaataagg tttacaaaca
       61 atcaagttta aataggggtg attatttgaa tactaaacaa ttagaatatt ttatatctgt
      121 agcagaaaag ctaagtttta ccaaaacagc agaaaaattt tatatttctc aaactgctgt
      181 tacacagcaa ataaaagctt tagaagaaca aataaatgta actctattta caagaagtaa
      241 acgtcatgtt gaactaacac cagctggaaa agttttttta tctgaggcac gtactataat
      301 aaaaaatatc aatgatgcta ttgcaaaaac acaacaattt gcacatggtt tttttggaac
      361 tttgagcatt ggaaccttaa taggatatga aaaaaacaaa cttcaacaat atttaaaaga
      421 attttctaat acatacccta atatatctat ggatatttgt actaatgaaa taaccgaact
      481 tttaaactta gtaaaaaata attctatgga cctcgctttt gttattaatc cagaaaatca
      541 accattaaaa gattttgaat ataaaactgt agaaagatat tctttggtag ctcttttacc
      601 tcctggtcat ccactttaca atgaggattc tatcgattta attgaattac aacatgataa
      661 atttattttt gtaaaggaaa caggggatga gtatggacaa aaatctatga tacaaaatag
      721 atatcgtgaa gcagggtttg tacctaatgt ggtacaacgt tcaaataatc ttaatactat
      781 tgaatcattg gtatcttcta acatgggaat atccatttta ccttcatttt gtgttacaga
      841 tacaatgata gaacgtgata ttgcaatagt acctataaaa gaagtagaaa atagaattga
      901 agttgtagtt gtatggaata aaaaaaatac aaatccagca ttagaaaagt tcatttctat
      961 aatataaaat taatcaatat taacgataaa aatagcatta accatttttt ctatagttaa
     1021 tgctattttc tactatttta ttatttaact tttaatttaa tatttataat cattaaaaat
     1081 aactctgttt aacaaatata agcaagtcaa aaatgtgtct aaaaaagata aaaattttat
     1141 tatatacaaa tgtattatct caatatatct attttttcat tgttcatcag tctaagtaca
     1201 tgacctgtgc ttactaaaga attatcccca taagttgtat gagctaacac cgaactagca
     1261 accccaaact caactatatc ttcactcttc atattttcca aatatgaata tataattcct
     1321 gctacatatc catctccggc accaaccctg tcatatattt ctaacttata ctccctactt
     1381 tcaaaataac catcatcatt tgatatatat ccctttataa atctattatt catatcatca
     1441 aatcttctca tagttccagc tacaatacct atatcatact cttttttaat caattctgaa
     1501 ctagatttta tatttagact atctatatta agtatcttat ttatatctct ataactagca
     1561 aacagcccat gacaatatgg tagtattttc ttataatcat ttactaaatc atcataagtc
     1621 ctttctggat ttaatgattg tctaaaatta aaatcaaaga atacttttat ccccttttca
     1681 taagattttc tagctagttc taaagcaatc tctttaactg aatctgtaag ttgcatagca
     1741 ataccacaaa tatggactat atccattcct tctaaacatt tatcaatatc ataatcttgt
     1801 aatttactct cacaaaatga actatttaac ctattcaaat aagtcacttc agaaggtctt
     1861 tctccatacc cattttcaag aaaatacact cctatatgat tccctccata tgtgataaag
     1921 tcatctttta ctccaagctt tcttatattt gcacttaatg ctttacctac attattatta
     1981 ggcaactttg tcaacatata ggtattgtat ccaaagtttt taagtccaga taatatattt
     2041 aatcccgtgc ctgtaattat ataatttagt tcattggttt gtgttaagaa cttgtagtca
     2101 ggtactgaaa gtctcatcat tatttcacca aaagctaata tattcataaa tctatcccaa
     2161 taattctttc attattttat aaagtactcc tatatcttcc actttcgttt cacctgtttc
     2221 tttatcaatt attgatgtat aaacatgagg tataactttt tctacaccag catcaagtgc
     2281 aatttgaact atctctttga agttatctaa gtctatacct cctgttggtt ctaaataaaa
     2341 attatgcttt gcacaagctt ttgcaacttc tatatattca tcctttgttt taagtcctcc
     2401 cattggaaaa aacttaattg agcttcctcc catatctttc atcatagcta ttgctgtttc
     2461 tataggaact ataccatctt tttcttttga acttaatgga cctgtggata tttttacaaa
     2521 tcctggttta ccagttggag aaactaatcc atttattata ctatcatttt gaccaagtaa
     2581 tgctctagac gtagccacac ctgtaaacac ttgatttata tgttgtggct gaagttcttt
     2641 agaaatctga gataccatat ttgactgatt tgggtctcca gctccaagcc ccacagatat
     2701 tgcattatct atttcctttg agtatttttt catatcttca atagctgctt ctacagtact
     2761 gtaattcttt gatagtactc ctatcaatac atgcccgtca caagcatcat atacttcttt
     2821 agcatttttt atgcttcctg caagaacatt taaacaaact ctatttttat aatattgtgg
     2881 tatacaattc atatttatcc cctccattgg taaaatttaa tttattaaat cattataatg
     2941 tcacttactc tacactctaa ctttctacta tattttttat cctattataa atagtatcta
     3001 aatcacttcc agttacactt cttatatcaa tctcaatctt gccttcatta gccttatagt
     3061 ctctagtgta tatagctatc tctccacttc taagttcatt tataatttgt tgtgttgact
     3121 tatttattat atcttcatta aagtcaattt ctccacgaag tatttctcta cctgctgaat
     3181 ccctagttat actagcactc actcccttta tagtatttat cttgtctata aatggattta
     3241 gcttgtcctc catttctttt aaagttactt tatcattact tatatatctc tcaattgcac
     3301 atgttagacc tacaatattt tcttttccta ttttcatagc tcttcctata ccctttgatt
     3361 gaagctttac attatctatg tattttttct tacctattac taaccctgag ctaggaccct
     3421 ctatagcctt agcaccactg tatatcacta ggtctgctcc caattcataa tatacattta
     3481 aatcttcttc tgctgctgca tctactatca aaggtaaatt atattcttta gcaacttgta
     3541 ctacatctaa tacacttagc atactctttt gaacacaatg atgagattta atatacataa
     3601 ttgctgctgt attttcattt atctctctta ttatatgttc cttgttacac atattagcat
     3661 aaccggcctc aacaacttct cctcctccaa gtcttatcat tacttctact ggaactccat
     3721 aatcaacatt atgtcccttt ggaattatta tttccctttt aacttgtttc ttaggattat
     3781 ttacattgta aactagactc atatcatctt ttgctatcat tgctcccaca ctctgtgcaa
     3841 tcccagcaga tgcactagaa actattagtg cactttcaca ccctaataaa tttgctatat
     3901 attcacctgt tttatttata agttcttcta ttataaagaa atttttacac ccttcaccta
     3961 ttgcttcaat aacttcctta tctactgttg atactcctag tatagtcatt ttgcctgaag
     4021 catttataac acgtcttaca gagtattttt caaatatact actcatacaa tagtttctcc
     4081 cttctactca ttctcttcta tagacttacc tatatttaaa tattctccat tgactactac
     4141 tgctcttggc tttatagacg tacttgtgat tactgactta ttcaaagaat cttgaagttc
     4201 tttttcttca ttaaccacat caaatatagt taaatcagca tcatatcctt cttttattaa
     4261 acctttattt tttaaactta ttgcatctgc tgcattttta gtaactttat ttattatatc
     4321 ttcaagtgaa tagcccatat atataaactt ttccattgtt gtagatagat tatacactgg
     4381 accatttatt ctatttttaa tatatatatc tgtacttata gtgtctggaa aaattcctgc
     4441 atccttagat ttcattgcaa tatccatact aaaactttcg gttccatgac ctacatcaag
     4501 gattatacct ctttctctag cttctatgat ttctttttta acctcattgc ctctaagtat
     4561 tccatttggc tttccattat atatatgggt taatatatct cctttttcca tatagtcaaa
     4621 tatatcctct atcgttggag gagaactccc aaagtgaacc atcataggta gattaagttc
     4681 attttttatt cttttagcta cttttaaagg ttcaacatca tttgaaatta caacactttt
     4741 actcattcta gctttgattc caacaacaaa atctttgtat tttttaactg ctcttttaag
     4801 ttcatattca tcaatattca tcatacttga aagttcatct tgagatgtta ttccctgctt
     4861 agcaatattt attaaagcat atacatttgt tttatatttt ttagtatctt cataaaattt
     4921 acctatatct aaagctcctg ttgttcctgc atctactaca gtagtaactc cactttttat
     4981 tccaacctca tctggataat ccttataaag gtctagtctt tcgtagcaat gcgtatgtat
     5041 atctatccaa ccagcactaa taaatttctc tccatctaag tttataattt gttttgcctg
     5101 atacttagaa tcatctatct gattttttac ttctattatt ttatcgtcta atatagctat
     5161 atcaattata cttttatcta ctgtttttcc acattttatt aatatgtcta tcttcacttt
     5221 actctccctc taaaataatc ttaaatttac tccaaggttc aataaagtct aaaagtatta
     5281 tttcgtcttc ctttattcta cccaccttat tttttctttc atctgtacaa tcttgaagta
     5341 tcaactgtaa ttccccttta tactttccaa agtcattatt accaatcact acatctcctc
     5401 ttttaaagag gttcttatta tcatgtttag ggaaattttc tttagcatat ttttttctta
     5461 cttctgtaga tcttgctacc tgattagtta tatctcctct tctaaagtgt tgctcattta
     5521 ccattattat cttttctact tcagaagttt tttcttcagc tactacatta aatacaactt
     5581 ggtattcatt tacttcagac attcttttta attcatcttc acttgcatat gcattcccta
     5641 caattacatc atcaatcaat cctgttgcga ataagtgctt agttgctgta gcaatatcta
     5701 agtttctatg catttcaagc gtacacagcc catcatttat attccaaggc cctattgttg
     5761 cgtattttga attaacaaaa gcagcagttc ttattccata ctttttaaat ctatttgaac
     5821 atcttacaaa aaaatcatat ggcaagcctg tacccctttg aggataaaaa ttgtgacaac
     5881 catatatatt atttacattt ggcttgtaag taattatatt atccaagtat gctacatcat
     5941 tactcatatt tagttccact tttaaatcat atggattgaa agaaatcata gcttctttac
     6001 ttccatcata tccatagtca agccttattc cactagcatt taactcacta aaaaatgtta
     6061 aatcatcata agatatttgt aatatatcaa atatgtttgg agctatatca aggattactt
     6121 caaatcctaa ttctcttgca taggttataa tttctttaaa atgtttttta acttcttcct
     6181 taggtcttgt aattgaaagc atgcacataa atattctttt aaatccgtat tttgaatcca
     6241 tttttatata tgccttatca tcttccatgt tactatgctc tggatatact gatattccta
     6301 atcttctttc catctttctc ctcctcaaat tacattattt aaaatatata aaattaaagg
     6361 caaaggtcat tcctcctacc ttcaccctta ataacttata agctttttct atattttata
     6421 tagtacaaaa cctctatatc tatgtatttt tataaatttt cactcatttc ttcttgttct
     6481 ctttgtaatt gttgtttttc aaaaactttg aagaatggat aataaacagc aaatgatatc
     6541 acaaagttga ttaatactaa gatacctgct agtatactcc agttagtact cattactgct
     6601 gctattggtg ctggcattgc aaatggtaat cttgctacca tcataggtat aacccctgta
     6661 atagtaagaa catatgatag aattgtcatt attataggtc caattataaa tggtatccct
     6721 agtattggat tcataactat tggtgctcca aatattatag gttcatttat gttgaataac
     6781 cctggtaaaa atcctaatct acctaaactt tttagatatt gtgacttaga aaacataaac
     6841 attacaacta atgcaagagt agcccctgct ccccctatcc atacatacca ctgtaaaaat
     6901 tgttctgtaa atatgtttgg cattgcatga gctgatactc cttctgtgaa tgcagttata
     6961 ttttctgcta tagacatgtc ccatataggt cttattactg gccccattat agctggacca
     7021 tgtattccaa gtacccagaa taaagttatt aggaatactg tcagtaaccc accacctaaa
     7081 ctatttcctg ataagaatcc ttttaaaggt gataacatag cgcttagtgc aacacttata
     7141 tcaaatccta aaatatgtct tattacccag aataacatta aaattactgc tgtcggtaat
     7201 aatgcagtaa atgaatttga aacctctact ggaactcctg ctggcatctt aattgtaata
     7261 ttcttttcct tcataaaacg atatatttca actgtaatta acgatgttac gatagctcca
     7321 aataatgatg gcgaattaag ttttgccata tccatccatc taccagcttc tataactcca
     7381 cctactggtt caactacttg agttggaact attgtagaaa ctaaaaatgc cataactgca
     7441 agcataccga cacctgttgt atctaaatcg tagcttttag caagtgatga tgctatccca
     7501 aaagtagcat atagagataa aaatcctaca gtatatctaa atggtatatc taatattgct
     7561 ttaaatggag ctataaaatc catataagca tcaataggta tgtttaataa tattgtgaaa
     7621 aatgacccca ctattgttag tggtaatgtt gctattatac ctgctcttac agctctcata
     7681 tgtctctgat tacttatctt cgctgctaca ggcataatct tgcgctctaa aaaacttgcg
     7741 aatttttcca tagttatccc cctgtattat tgatattatc atttatttat aatgcaataa
     7801 cgatttcata acataaacta atttttatgt aattgataat atcacttatt tattaaaaat
     7861 attataacat tataatgttt ggttgtaaac attataatgt tataatattt ttttgttttt
     7921 acaactaaac aaaaatcctg agcatattat catttcaata aactcaggat tcttattata
     7981 tttcgtattt aattacatag ttttgtactt ctgtattata tcttgcaaat gatagctcaa
     8041 ctatattatc attctcatca taggtctttc tttttcttcc taataatatg tctttagcta
     8101 gatttagagc ctttgatacc tctagttctg attcttctat aaaaaattca tcttcaaatc
     8161 tagttatatt tatatttctc ctatataagt acatatataa agaactttca ttattaaaac
     8221 tttcaacagt taaattttca tcaaaaggaa tataatgagt aaaaaatata tatggcttac
     8281 tatctaagta atacattcta gtaatcttgc tacatttttc tccaaatata ctaaatactt
     8341 gtgaatttgt atcatttatt attttttcaa ttgaaatagt ctttttagat aacttatgac
     8401 ctttagataa aagtatactt gaaaaagact ctgcttttga tagcttatta aaaagactat
     8461 tacttattac ttttgtccct tttccacttt ttttctctac atacccttct gattctaata
     8521 tctcaatagc ttttctaata gtaattttac tgactccaaa ctcttgctct aactcccttt
     8581 ctgttggaat attttcatca attttatatt cgttatttaa tattttttgc tttaatatat
     8641 ctacaacttg tatatataat ggttgatttc ttaacatgat tcacctctta aattatatgt
     8701 tataatatta taacatataa tcttatatta ttaataataa atatttatta actttaaact
     8761 aaatataaaa agagtcctca aaataatcct aatgtattac tcactaaata ttgaatcaat
     8821 aatctaacaa ttagtgtgct acacataatg tatcttttaa gaactctctt aatttattat
     8881 ctttaatttt tatttatctt gtaatttcaa atgtaaatca acgatttcct cagctaactg
     8941 ttgaaaattc atgccattca ttaagtggtc ctgtgcatgt ataagtatga cacttatttc
     9001 agtcctgttt ccactagctt cgttttgtat taattctgtt tgatatctat gtgctttatt
     9061 tataacttcc ctagactctt ttaataattc ttttgctttt tctatatttc cattcttagc
     9121 ctcttttatt gcttcaaaag ctaatccttt accttcacct gcataaccta ttatattaaa
     9181 agatatttct ataatctttt catccatcct ttgattctcc caatttatat attaaaaata
     9241 cattataatt tatattactt agtgttgtta agttctatcg ctctatttaa aacagcttct
     9301 ccattaactg ttccatagtg catcatattt ataacttcta ctggtatatt atatggccta
     9361 gcaatttctt gagccttagg taatacatat ctaacttgtg gtcctagtaa taatacatca
     9421 catttttcta tttcattttg tatagttgat tctggtattg cccaaatttg cgactctata
     9481 ccattttctt tagctgcact ttccatttta gtaactaata aacttgtaga catccctgct
     9541 gaacaggcca acattatttt tatcatatat tttcctccaa atttttatta acaactatat
     9601 aaacattata atgttataat attttattta attctacctt aaatgcaata tactttcaat
     9661 acttttttgt tattttaata ttattaagtg tcaaattaac aaaaatgggc tgtcgcatta
     9721 gcaaattaaa tttgttctgt gatagctttt tttacttatc ttaattagat atatttttaa
     9781 ttgttttata taaagcttat gttctaagta aaattatgca taacaaataa aggtataaaa
     9841 ataccatttt ttcagataat atctaactat aaataataaa tttagaatac ttcttttaaa
     9901 tgatgaagat atattccaca tttatttttt attattttgt tgtgtagtct agaaatgtta
     9961 tgagctatcg ctacaataat acattctgat ataacatttt ctttgcctca gcttaaaaat
    10021 cttctaaatt tcatattttg ttttacttga gcgaaagttc cttctgcttg tataattcta
    10081 ttcattctaa gttgacaacc ttcttggctt ataattctct ttaaattttc ttttcttttt
    10141 tcatgaaata acttagatac ttgtaaattt ttagttctat tttcaagagg aatcttacta
    10201 ttatttccct taatacaacc gtttttatat tgacaatcac agcattcttt acaagtataa
    10261 caagttattt tacttttata tcctgttttt gattttctat tgattatttt attagcaaat
    10321 agttttttgc catttttaca aatataataa tctttttttt catcataatc catattatca
    10381 atacgcccaa tatcattttt atattttcta gttttactaa tttcataatt tgcaggcttt
    10441 ataaaagata attgattatt acactctaaa aatgtatagt tttcctcact ttcataccca
    10501 gaatcagcta ctattttagg atatttaaat tttgtatgct cttcaataga ttttaaaaat
    10561 ggtattgatg ttgtagtatc agtaggttta tgctctgaac ttacccaaac tatgtattct
    10621 gaatctacgc caaactgtat attatatgct ggtttcaatt gcccattttt catatggtct
    10681 ttcttcattc tcatgaaagt tgcgtcatta tctgttttag aataactatt tagttcaccc
    10741 attatatgaa ggtctttagt atattttttc aatctatcga tataattatt taatttcgct
    10801 atagattttt gaacaataga ttttttcttt ccttttccat gaacaaatgt tatatttgat
    10861 tcttttgata tttttttaag cttctttaaa agttttttta aatgatatat tttgatttta
    10921 tttctataaa ttattttaat accaaaatca atcccacatt ttaatatgaa attagatata
    10981 tcattcatca ctctaaccat atttttgttt atagtttttt tccaaacaaa agtatatcta
    11041 ttggcagatg actctatttt tgtaccatca acaaatatag catcttttgg aatcatataa
    11101 tcaatattta gtggtaattt aaattgataa ttattaccat aagtagtata atcaatttgt
    11161 atagtatttt gttttagcat acaattatta tacaggaatc ccaggttctt ttgaacttga
    11221 gctttatttt tttgtataaa aaagggcgta gcactatttt ttagtgcgac agcctttttt
    11281 ctgttatgta ccctaaggaa tgtaattata taaataaatt tatatataat ttaaaaacaa
    11341 tgtaatattt ttatttaaaa tttcagcaat ttgatttata tttatatttt ctttgatatc
    11401 attttctact ttatagctaa atttatattc acttaatttc ttataaaatt gaacaaagga
    11461 aatatttttt atttcgcttt tatcaaatat tgcataaagt acatgagcta ctttacattt
    11521 taacaacata ttgctgagat cttcaatatc aaaaacatta tcgcctagta ataaaacata
    11581 ttgtaatgta actctatatc ttaaataagg agttaggtta aatcctttac atgacaatat
    11641 aatatctaat gcctgagtta taaatccata aattatcagc caaaatttaa aagcatcact
    11701 tttatgtgct ggtaggggta ccttcccctt atcaataaag cttttaaatc tttcttccag
    11761 atttttagtt aaatcttcac cttcgtcaaa aacaatataa ctctctatac ctggattcaa
    11821 tgctagagct atatcttcga ttgagttaaa ttcttcaaag taagtatcta agtttaagtc
    11881 ttttcttgaa aaaaagttca gaaatatatt atttgaatat tcatgtatta ttttcatgtc
    11941 attaccatat agtaaattca ttggtaatat taacatcgca aaagaataat tagacaatat
    12001 tttgatatta tcatctatac aagaaataat ttggtctttt aatactttag caaattcctg
    12061 atttgataat actagtactg tttttgaata tttacatagc gaatcatcta ctatatgtat
    12121 atctcccatc ataacaaatg gataatgttc taaatcatca acatctagat atgttgcacc
    12181 tgtaaaaata tatgcattgt ctttttgtgt aatattccct aagtaaagct ttataacatc
    12241 tctatatgaa taccaaaata tatttatttc atctattata taaatataat ctatagtatc
    12301 tatattatct tttaacttta ctaataattc taaatattcc ttctgaatac tatttatttt
    12361 atttagtaaa aaattctttt ccatttttta tcacctctaa tagcaaatgt tgtggtatac
    12421 catatacagt atctaagaaa agtaaagttt tttccattat gtttacacaa tcatgccaaa
    12481 cactttttaa attttgatta ttgtctacat ctggtataca atgatagtga tgagctggat
    12541 tatcacactt taacattgaa ctttctctta ttgatcccca taaaccatgt tcatatgcgg
    12601 aatcataatc atagtacaac ccatataact ctttttctcc aactatttca gccttactac
    12661 gtatattttg cttatcaaaa tatttagtat ccatatctat tgtttcttca attacaaatt
    12721 cattaactaa agcttcaata tatttataat caacatgact attttttaaa tcttgttctt
    12781 gttctctata tctagccaca attaatttat attgacctat tccataaaat tgatattctg
    12841 accatatatt ttcatgttga gcttctattt ttactaaata cttcatcata atataatttt
    12901 ctatcaatac ccttactgag cctctacctg aaatggaatt aaataattta tgctcaacta
    12961 cttctcgtaa tcgtttataa gaatatattg ctattccaag taatactaac attttctcgt
    13021 ctaatggatt tactttacat agtaaatctg ataaataaac aaaaatctca tgtaattgtt
    13081 ccatatacaa gtctgtatta gcagtttcat cttcaaattt agttgcgtat aatttacatt
    13141 cagtcataac actaatcctc ccccaaaaaa tatctaaata ccaatcagat tttttttcca
    13201 tatttaaaac cattaattct gctgcacgaa taaacggacg taacattctc attttttcat
    13261 cagtgtgttc taaattagga tattccagca aattattaat ctgttctttt tgcaatacga
    13321 gttttcctga catacaaaaa taatataaaa ctataaatct tatgtctgta gcaaattcac
    13381 tttgatgatg catagctttt cttaaagtct catctaaata ggtaacccta tcttcaattt
    13441 ttatacttgg ctctgcaaaa tatttaacaa aaataggata attactatat gtaaatatta
    13501 atgtcaatgg agataaagct tccttttcaa caatagttag aatataacta aagaattcat
    13561 cctgctttac atcctccata gataaaatct cagacaacct aggtgttaca atatctgatt
    13621 caattgaatg aagttttaca attattttat aaattatttc catccctttt tttctaccat
    13681 atttatttat taataatcct atccatatat actctggcaa tcgaccaagg caccatgact
    13741 cattactatt caatggtgtc ataaattcat taaaaggtgc tataaatttc ccctttttta
    13801 atttgtgatc agatagttta ctatgactca cattcttccc tcctatattt taattatata
    13861 taaaaataaa ctaatacgtt aatactcctc atcaattaca attttactta atcattattt
    13921 taatcgctaa tatttaaatc tcataacact tatgtactac cacattttgg ttaattgtat
    13981 cattatctta tatataaaaa ccgtattgct taattgcaat acagttttat ataaatttat
    14041 aattttaata tgtattatcc ctttatccat ttaaccactt ttctaattta aatttttctt
    14101 ttgctatttg tagtacactt acttgccctt tgctattttt caatattgct cttgcatatg
    14161 caaaaaatac tttttcatta gcaaacccat ctttcgtttg tttatacaat gtatttgcat
    14221 acgcacgaat taaattatct tcatcataca tatcatataa tcttgcatgc tctacttctg
    14281 caattgtatt atttctaaaa ctactatcat attgtattaa aaatttccaa gcttccccta
    14341 attcattgtt tctttcatct accacacaaa aaatattatc tataataatt tcatcttcag
    14401 taatatcttc atagcaacta tcacatattc ccactaaact tgataaatca tttatatttt
    14461 tatctattgt tagatatcct atctttaagg gtcttcctga ttcaccacaa tgataacata
    14521 tatatttttg cttattgaat attagataag ctacttgatt atttatattt tgagatttat
    14581 ttattgattc tagtttagct aaattttcaa tcaaaacagg gtgtacttgt aaattattgc
    14641 tcctttcatc acaatacaat agttttttct ccataagtct tctaataatt gctcttattg
    14701 gcaatagctt tgttttttcc attaactcat atacaactgc ttcactgtat gtaatccccc
    14761 tcatatttat taaatgttct ttgcctcttt cccatgtata agtattttca ttttgcttgc
    14821 aatctttaca cacagttacc aataaccttc tgtctaatct tcctagtcta acataattat
    14881 taaataattg cttgaaaata ttctctcctc tatcttgcat atctactaaa tcactaatta
    14941 catctatact ttcactctcc gtaattatat cttcgagttt aaatttaata tctttttcgt
    15001 ccttaattga tagtgtcttt agcaacaatt cattttcttt attttttatg ttagccatca
    15061 ttgcagctct ttgttgccat aatctatatt gttcctgtgc aatttcaacc gattcatact
    15121 gtgaattaca tctatcacac ctaatattat actccattat tttatcatta atacacacat
    15181 ataaatctga ttggcatcta tgacatttaa tcttgtttat tttattatat acattctcca
    15241 atgttcttat aaatagttct ttttttcttt tttcttcttc tattatatta tctacttctt
    15301 ccttatgaat aaaataacta attgcctcac cacacttttc acattcaact
//
LOCUS       DAELNY010000083        15234 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.083, whole genome shotgun sequence.
ACCESSION   DAELNY010000083 DAELNY010000000
VERSION     DAELNY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.083"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            108..692
                     /locus_tag="KPE55_002171"
     CDS             108..692
                     /locus_tag="KPE55_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG0493234.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(796..1575)
                     /locus_tag="KPE55_002172"
     CDS             complement(796..1575)
                     /locus_tag="KPE55_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0493235.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1633..2889)
                     /locus_tag="KPE55_002173"
     CDS             complement(1633..2889)
                     /locus_tag="KPE55_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG0493236.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3147..4046
                     /locus_tag="KPE55_002174"
     CDS             3147..4046
                     /locus_tag="KPE55_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG0493237.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            4070..5518
                     /gene="ddlR"
                     /locus_tag="KPE55_002175"
     CDS             4070..5518
                     /gene="ddlR"
                     /locus_tag="KPE55_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG0493238.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5577..6383
                     /locus_tag="KPE55_002176"
     CDS             5577..6383
                     /locus_tag="KPE55_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0493239.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6409..8328
                     /locus_tag="KPE55_002177"
     CDS             6409..8328
                     /locus_tag="KPE55_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0493240.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8570..8917)
                     /locus_tag="KPE55_002178"
     CDS             complement(8570..8917)
                     /locus_tag="KPE55_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG0493241.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8904..10832)
                     /locus_tag="KPE55_002179"
     CDS             complement(8904..10832)
                     /locus_tag="KPE55_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG0493242.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(11244..13433)
                     /locus_tag="KPE55_002180"
     CDS             complement(11244..13433)
                     /locus_tag="KPE55_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG0493243.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            13764..14495
                     /locus_tag="KPE55_002181"
     CDS             13764..14495
                     /locus_tag="KPE55_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0493244.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            14575..15210
                     /locus_tag="KPE55_002182"
     CDS             14575..15210
                     /locus_tag="KPE55_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0493245.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
BASE COUNT         5582 a         2292 c         1964 g         5396 t
ORIGIN      
        1 taaatactgt tgacttagtt tcatatattt accattaaac ttttatcata aatatcatat
       61 cataataata tatttaataa gtctaataaa ctatacgagg tgtaaatatg ttaatgaata
      121 cccattttat tatggcaaaa tcaatacttg ataatataga tgaaaataaa acattcttta
      181 taagcgaaaa aaattttata tatggcaata taaagcctga tgctttttct aaatataaat
      241 taaaaaaaca ttatttagat gagtcattta atatgattgt ttctaaaata aactatctat
      301 gcagtcttaa tatagatagt ctttctaaat acttttcagt aagtagactt agtcaggaac
      361 taggagtcat atgtcatttt ttatgtgatt ttttctgtgt agctcacagt gaacgttggg
      421 agtttaagca tagcttaaat aaacatgtta cttatgaaag agaacttgga gcatttgcaa
      481 aagatgttga tttatctaaa attaaatgtg gtactataaa tagtagtttt gagaagtttt
      541 ttactaaact ttatagcgat tacaaaaaga aaactgatta tatgaatgat ttaaaatttt
      601 cagcttatac ttgtaatagt gtcataaatt atatacttga ctctatttta tctaatacaa
      661 taaaatcata tcatatagca aattgcggtt aacttaagaa tttttaaact tatataaact
      721 tataattgaa tataattaaa atgactgctc tatagaatat aattttatac ttctctagga
      781 cagtcatttt ttatactaac tatttatctc cattataaca tctatggcac tttttacact
      841 atctactaca taatttgcac tttgctttac atcttccctt gccttagaca ttgcaaaact
      901 atatttaaat ccttctaaca tgcaaatatc atttggagaa tccccaatag tagcaacttc
      961 atctggactt acatttaacg tttttattac atgctctata cttcctcttt ttgacacact
     1021 tttaggaact atatttaaag tatatgttcc agaaaaatat atttctaaga cttcactaaa
     1081 ctcacttttt agataaagtt ctattctgga taactcttcc aaattttcag aacatacgcc
     1141 tatatttcct ataagattat caaaactact tcttttttca aagtctgggt caactataaa
     1201 gtcactagaa taatgtctaa aagcagtggt atcactaccc acaattacat cacccttata
     1261 tttaaaataa agttgtgcat cacttgactc aaatctatca atgacttttt ttactaaatt
     1321 atcatctatc gttcttttaa aaattacatt ataatcttta tcatatataa atgaaccatt
     1381 ttcacataca tagtatccat tcatatctaa actatctttg ataatattat gtgcttgttt
     1441 aaaaattcta cctgtagcaa cattaaactg tactcccttt ttttctattt cttcaattgc
     1501 tactatatct tcttttgtta tggttccatt ttcatataga gttccatcta aatcacaaaa
     1561 tatatgtttt atcataaatt aaaagcctcc aaaaacaatt ttatatttta ctaatacttt
     1621 aatcaaaata acttaaagct tttctaattg tattctcaac tcattaagca aatctctagt
     1681 agctcttaat cttatctttc ttctatcccc attaaatata tttttcttta ctattgtttt
     1741 tccatttata tatattgcag tataaaccag tcccacaggc ttctctttgg ttcctccacc
     1801 aggtccagct actccagttg tagatatacc tatattagct ttaagtcctt tagcaacgcc
     1861 ctcagccatt tctatagctg tttgctcact tactgctcca aactcttcca aagtttctct
     1921 ttttactcct aatcttttca ttttagaatc attagaatat gttacacaac cttcaagaaa
     1981 tacagaggat atacctggat agtttataag agatgaagaa accaaacctc cagtacaaga
     2041 ttcagccact gctattgtta gatttttttc tacaagtagc ttagaaacag catcttctaa
     2101 agcagtctcc ccttcagata tggaaatatc tccttcagta tatacatatt taccaactct
     2161 actttttatt ttttcttcta ctggttttat taatttaaaa gcttctcttt cattttcagc
     2221 ctttgcagtt attcttattg taacttctaa ttcttttgca tataaagcaa ctgttggatt
     2281 agtctgctca ttaattatat cgagaatttc taattctaga tttgactcac ctattccata
     2341 taatcttaaa gtcttagata ctagcatttc atttgtgaat tgttgtaagt aaggtttgac
     2401 actctcatta aacatagctt tcatttctct tggaggtcca ggcaatacta ttataaattt
     2461 accatctttt tttaaaattg cccctggagc tgtaccatta ttatttttta gtactatagc
     2521 atcttttgga aagtacactt gtttcatatt attttcggcc atttttttcc ctgttttaac
     2581 aaagaaagat tccaaaacct ctagtgaagg tttatgtagt tctaatttct gaccaaagta
     2641 ttctgcagca gtttcttttg tcatatcatc accagttggc ccaagaccac ctgtagtaat
     2701 tacaaaatca cttctcttta agctttcatc aaaacattct aaaagtcttt gtttgttatc
     2761 tccaacagta ctctgatggt atacttctat tcccaatgat gcaagttctt tagctaaaaa
     2821 ttgagaattt gtatttacta tatctcccaa taatatttct gttcctacac ttattatttc
     2881 agctttcaaa atttatcact ccttgattta tagccattaa gactagtctt aagttcataa
     2941 caataatttt agcaaaaata ataacataaa taaagttaaa aaatcattta aacatatctt
     3001 taaactagat tattattcta aaaccacaca taatataaac ttcgtatata gtggtacttt
     3061 catcctcaac tgttaattca tataattaag tatgtaaata aaaagtttac tttaaaataa
     3121 taaaaattaa aataggagtg tataatatga aaatagcagt tataatgggt ggaatatcat
     3181 cagagagaga agtttcttta aattcaggaa aagagatata taataacctt gataaaaata
     3241 aatatgaagt tgtaaaagta ataatagatg ataaaaaaga tatatttaca aagataccag
     3301 aagatataga ttttgcaata cttgctttac atggaaaatt tggtgaagat ggatgtatac
     3361 aatcaatact tgagactatg gatataccat actctggatg tggacctctt tgcagtggga
     3421 tgtgtatgga taaaaatata actaaaaaaa tgcttagaga ttcaaaccta cctactgctc
     3481 catgggtttt agtaaaatca gttgatgaaa tagactatga tgaaatagat aatataggtt
     3541 atccagtatt tataaaacca aactcaggag gttcaagtgt agccacattc tttattcatt
     3601 ctaaggatga agttgaagaa gcagttagaa aaggtctgga agttgatgaa tttgtaatga
     3661 ttgaaaaata tataccaggt ggagaatata cttcattcat attaaatgga gaagtgttcc
     3721 ctacaatatc tataaaatct gattcaggat tctttgatta tgaagctaag tactcagttg
     3781 aaaaaggtgc aaaggaagaa gttgtttact tagatgaaga acttcaaaaa agagtaaacg
     3841 aaatttctga gacttgttgg aaaattttta attgcaaagc atatgtaaga gttgatatga
     3901 ttataagtga aggtattcca tatgttcttg aactaaatac tcttcctgga atgactcaaa
     3961 ctagtttaat tcctagaagt gctgctgcta gaggtattaa gtacagtgaa ctattagata
     4021 aacttataga atactcttta aattagatga aataatttga ggtggtaatt tgatattttc
     4081 aaatctaaaa ctaaatgata atgaaccaat ttatatacaa cttaaaaatt atataagtga
     4141 tatgatatca aaaggactaa ttccagacaa tagtaagctc ccttctacaa gggagcttag
     4201 tcagctttta caagttagcc gaaattctgt agttttaact tatgaagaat taaaatcaga
     4261 aggattaatc tactctattt caggaaaagg tacatttgtt aaatcaaaga ataagtcttc
     4321 aaatactaca tggtcattaa actgggattg tttagagaat acatactcta aaaaagccaa
     4381 tgaattggac attataaaaa gtgaaattcc ttggagctct gacctgattt cctttaaaag
     4441 tatttctcca gatggagatt tattcgacat agaagaatta aaaaaatcct ttttaaatag
     4501 aatttcacta gaaggacata aattactcaa ctatggatat gcacaaggtt ataagcccct
     4561 tattgactat ttactagaat atatgacaaa taagggtgct gatataagta ataaagatat
     4621 actaataaca aatggattca cagaaggctt tgatattatt atgtcatctt ttacccaaga
     4681 aaaagactac ataatatgtg aaaatcctac tcataatact gctataaaaa ttatgaaatc
     4741 tcataacctc aatatcttag gtgtagatat aaatgaagat ggtttggact tcaatatgct
     4801 agaagacaat ttagttaagt ataaaaataa aataaagttt gcatacatta caccctctta
     4861 tcacaatcct acaggaattg taatgactcc tgaaaatagg tacaaattct ataatcttat
     4921 gaaaaaatac aatatagcaa ttatagaaga tggatttaat gaagaattac tctacaatag
     4981 ttctcatata tttcctatct gttccctaga taatatgaat aatggggtcg tatatattgg
     5041 tagtttttca aaaattttat ttcctggaat gagaattgga tggatatttg cagataaaaa
     5101 agtaatcaat aagctagaaa gtgtaaaaag gtgcagaaac atacatgtat cctttttgga
     5161 ccaaggaatt ttatttgatt atttaagtaa tggaggtttt gaaaaataca ttaaaaaaat
     5221 acgtaaattc tatggggaca aatttaactt tgcatataag tgtataaaaa aatatataaa
     5281 aacagaatat atcttgggtg atggtggttt gcatatattt ataaaactaa aaaatataga
     5341 tactagagtt ttattagaaa aatgttatga aaaaaatgtt atatttatgc ctggagattt
     5401 attttttaca gataactctg gttttgatac tttaagactc ggattttcta ggttgtcttt
     5461 tgaagacatt gaaattggaa taaaaataat tggagaaact ataaatgaaa tgttctaggt
     5521 taactaaata ttgttagcct agtttatttt tatgaggtat aatatatata gtttatatga
     5581 atctaatact gggggataat atgagtaaaa agtcaaaacg aagaaaaatt aataaattaa
     5641 aattaacgat tgtaataact gtattatttc ttgctttatt atctatatat gagatactgt
     5701 tctctaatcc caacaaaaaa ggtaatataa agagtcaaga aactatgcaa aataaaaata
     5761 atacaacaga tgattctaaa aataatcaaa gctctaccaa aaagcaggaa gataataata
     5821 tcaagcctac tttttcatat tcttcaatac cagataatat aaaaaataaa atgattggta
     5881 aatctatgcc tactgatgaa cctataagct ttgatagtct atcttattta aaacttacat
     5941 attatggatt tgatgaaaaa actcatcaag gagaaatgat agtaaacagt gaactagctc
     6001 cagaagtagt ggatattttt aaggaattat acgaaaaaaa atatccaatt gaaaaaatta
     6061 agttaataga tgactatgat gctgtagatg aaaaatctat gtctgataat aatacctcat
     6121 cattttgtta tagaactata gctggtacaa atgtagtttc aaatcatggt aaaggacgtg
     6181 ctatagatat aaacccatta cagaatcctc aagtttctgg aaatgatgtt acacctaaag
     6241 taagtacagt ttatgctgat aggtctagca caaaatttgg aatgataaaa aaaggtgatg
     6301 attgttataa tgcttttgta agcagaggtt ggagttgggg tggctattgg aaaaacccag
     6361 attatcaaca ttttgaaaaa taatagatta gaaataaagg tgaattatat gaatttaatg
     6421 acattggaaa atataagtaa aagctattct gaaaaaaaat tactagaaaa tatatctctt
     6481 ggaataaatg aaggggaaaa aataggactt attggtgtaa atggtactgg taaatctacc
     6541 ttactcaaaa ttattgcagg agttgaagaa agtgagactg gtaatataac aaaaactaat
     6601 ggtattagga tagaatatct acctcaaaat ccaacttaca atgaaaattc aactgtactt
     6661 gaacaagtat ttaatggaac ttctgaagaa ttaaagctac ttggtgaata tcaagaaatc
     6721 ctagaaaaac ttaattcaaa ctttacagag gaattaaata aaaaattatt atcagtacac
     6781 gaaaaaatag atgccttaaa tctttgggat ttagaaagtg aagctaagtc agtacttaca
     6841 aagcttggta taaatgactt taatcaaaaa atagcagaac tttctggtgg gcaaagaaag
     6901 agagtctctc tggcatcagc ccttattaca ccttgtgagt ttttgatact agatgagcct
     6961 acaaaccatc tagacaatga tactatagat tggcttgagg aatatcttaa ttccagaaaa
     7021 ggctcattgc ttatgataac tcatgataga tactttttag accgtgttac aaatagaatt
     7081 attgaactgg acaaaggtag attattcagt tatgatggga attattcaat atttttggaa
     7141 aagaaaatgg aaagactttc gctagaagca agtattgaag ataaaagaca aaatttaata
     7201 agaacagagc ttgcgtgggt aagacgagga gctcaggctc gtagcactaa acaaaaagct
     7261 cgtttacaaa gatttgatga acttataaat agagattctt ttaaacctga tgaaaatctt
     7321 gatatatctg ttgcatcaag tagacttgga aataaaataa tagaaataca caatatatct
     7381 aaatcatttg aagaaaatac tgttatagat aatttagaat acactttaac tcgtacagat
     7441 agaattggta taataggcaa aaatggtatg ggtaaatcta cccttataaa tatacttaat
     7501 ggagaaatag agcctgatag tggatatatt tcaattggag aaacggttaa aataggttgt
     7561 ttttctcaag acgactctca tatgaatatt gatatgaggg ctatagacta tgtaaaagaa
     7621 gctagtgact atatagaaac ttcagatggt actaggatta cagcttctca aatgtgtgaa
     7681 agattccttt ttaatggtac tatgcaatat actttgatag gtaaattatc tggtggtgaa
     7741 agaagaagac tacatcttct taaaatcctt atgacagctc ctaatgtgct tcttttagat
     7801 gaacctacaa atgatttaga tattgaaacg cttaaaatat tagaagaata cctagacgaa
     7861 tttaaaggtg tagtagttac agtatcacat gatagatatt tcttagatag aatttgtaat
     7921 aaaatatttg catatgaagg taatggaaaa attcatatct atacaggtaa ttacagtgat
     7981 tatcttattt atagagagat tcaaggaatt gaatttgaag aatctaaaag ggaatcagtt
     8041 aaaaaagatg actctactac cccaaaaaaa gaaaaaccta aaaatgataa aaagcttaag
     8101 ttctcatata atgaacaaag ggagtttgaa aatattgact ctgatataga aaaactcgaa
     8161 tctaaaattg caacacttga tgaaagtact gcaaaatttt caactgattt tactaaactc
     8221 caagaaattt tagatgagaa atctaaacta gaaaaagaac ttgaatacaa atatgaaaga
     8281 tgggaatatt taaataatct agctgatgaa atagctaata ataagtaata ttaaaataat
     8341 cttaagttta ataagttttg ctttaaatct gtaatttata aatattttaa aggaagtgtc
     8401 tcaaaatgaa ctttttagtt ttatgagaca ctctttttta tgagaaatca aatatatttt
     8461 catcatttca acaatacaaa agaggcttat ctctattttg agacagcctc tttcataatt
     8521 accttctaat ttatatgctc taatatatct ataataattc ttattatatc tatatatcca
     8581 gtctttcaat atagtgacaa ttaacttcaa tatgatttaa aaatactaac aacctttcat
     8641 aaagtggatg agattcttct ccatatttag cttctaggtt cttgcctatt tctctaacag
     8701 tcctttgacc atcaatttgt aaaaaaacaa aactggaata ttcatctagt tctatttttt
     8761 tgtatttagg tattctagct tttaactttc taaaaaactt ttgaatttta tggtcctgtt
     8821 tttcaagtat agtaacaaca cgattttcag ctacttcata ttcaagtcca tcagtaattt
     8881 taaatacaat atctaaaaca tcattactct tgctcattat tttttacttt agataaaaca
     8941 ataggtaatg ctgtaagcac aactaaaact actaataaag caattgccat tgaatttgtt
     9001 gcagcaaatc cacttggtac ctttggagtt acaactccag ttacttgtaa tattatacct
     9061 attaaaccaa tgatagaacc accagcaaca agaccagaag ataaacttat accattagaa
     9121 actttaactt ctttttcttt ttcagtttta gatactaatt caacaaataa acgaattaat
     9181 gctccaacta atattataga agtagttgca attggtaaat agaatcctat agctatagtc
     9241 attattggta aatttaatgc atatagaact attgccataa atactccaac aataatcata
     9301 acccaaggta aactacctga cataatacca gaagttaatg ttgacattaa gtttgcttgt
     9361 ggtaatgcaa attgtacatt atcaccagtt gctctaagtt ggtcagaaag taataaaatt
     9421 actcctacaa caactataac accaactata cttgcaattg taaagtaacg ttgcatttcg
     9481 tttttgctac caccaataat ataagttact ttttgagatt gagtataacc tccagctata
     9541 gcaatagcaa caactataaa tgaaccaaat aaaagtaatg atttattagc ttctaaatca
     9601 gtccatccca ttataacaaa cactaatgtt acaataacta gagaagctat agtcatacca
     9661 gaaactggaa gatttgatgt tccgattgtt cctgttaaac gaccagctac tatgacaaat
     9721 aataaagata ataaaagaga tatgatagct ccaataattg ccataacaat gttaccagaa
     9781 attaagaatg ctgctaaaaa tccaataact actccaccta ataaaagtat catttgtata
     9841 gatgaacctt cttctccttc tccagcactt gatttagctt taagtgtttc ttttatagaa
     9901 gcgattatag ttggtataag ttttattgca ccaataatac ctccacaaag catcatacca
     9961 gcacctatat attttacata actactagat attgctccaa catccatttg gttaagaggc
    10021 attgcaggat tattccatac catagctcca tctttagcca tatctgtaaa gtatcctatt
    10081 aaaggtgcta taccaaagtt tgataaaata cttcctgcaa acattgttaa agaaacttca
    10141 agccctacta taaatccaat tcctaaaagt agaggattta cttcaatatc aaatttccat
    10201 ttgtagaatt tgcttcctac aagactcatt acattgtttg ctacatttaa gaatgaacca
    10261 gttaatacag taataaatcc acttattaca aatccaattc ccatgtactt aattgaatct
    10321 ccaccagcct ctgaagcaac aagtgtctca gatatagcca ttgattctgg gtacataagt
    10381 ttaccatgtt cttcaactat tagatagttg tgaactatag cagcaatccc tataccaaat
    10441 aaaactccac caactccaac agctaagcct tctaagaatg taatttggct tccaattaat
    10501 ataactgctg gtaatacgaa aataaatcca cttgcaacag attctccacc acttgacata
    10561 ccttggatta agtttttacc aagaataccc tttgaacgag caaatgctcc aacaaaagca
    10621 gaacctatta tagctcctgg aatacctgcg gcaactgtaa gaccggcttt cattccagag
    10681 taagttgtag atgcggcaaa aatggcagct aaaataatac cgataatcag tacagctaca
    10741 tttccaccgg ttctggactt atctgtgata tatggaacgt aatcttttcc actaacctca
    10801 ccataagcac ccttaggtaa ttttttgttc ataaaatttt cacccctttt caatttgtat
    10861 atattataac atatatacat ataaaactta actttttatt attttacaaa tagcgagtca
    10921 aaactactaa aatttaaccc tatattatta atttctactt ttagatattt ttatgctaat
    10981 tttcttttta tttttcaatt ctactaaaaa ctattaagat ataaaataaa aaaatttttt
    11041 aatgtaaaat taattttgta aaaaaaatgc tttaaaacac ggaaaagtat aaaaaataca
    11101 ataatcattc ttataaataa attttcacca tagtaaatat atacagaaaa ttcttgtttt
    11161 tatgcaaatt gattatttta taaaaataag tgtcataaag aaatgtctga aatcacttta
    11221 tgacacctat cataataaaa aaattattct ggtaatgctc ttttatttac ttttccgatt
    11281 ggtgtaactg gtatcttatc tatcctcatt attattgttg gaatcatata tggaggtaga
    11341 tgctcattta aatattctct aatagttctt tcatttatct taccactatc ttcctcatca
    11401 attacataat atgcacatat ataaatatct ccatcatcat ttgttctcga tatacaaaca
    11461 acacttttaa tttctgtaat attatttatt atattttcaa tctcatatag ctctatccta
    11521 aactcatcaa tttttacttg attgtccatc cttccaatat aatctaaatt tccatcatct
    11581 aaccatctta ctaaatcacc agttctatac attaacttat tatgttcaca agttttgtaa
    11641 ggattctcta caaatacttg ctctgttagc ccttttttat ttaaatatcc tcttgatatt
    11701 tgaatgcctg atagacatag ctctcctggt gctccaattg gacatagttt gttcttttta
    11761 tctaatacat atgcataagt attaaatata ggttttccta ttggtatttt tccatattct
    11821 ctgtccacat gaaaagaagt cgatacaaca gtaaattcgg ttggtccata ctcattatat
    11881 agttcataat tacgctttct atatcctttt aatctttctc ctccagtctg tacaactcta
    11941 agtgaattat tattgcaatt ttgtataaat aaccttgcaa atgctgttgg caatgctagt
    12001 atagttatgt cattttcctc acagtattca tttactgtat attcatcaaa tttagcttct
    12061 tcaggcataa tatctattga tgcacctaca agcaaataag ggaatagttg tttcattaca
    12121 gcatcaaaac tgaaacttaa gtatatacca catatatcat tttctgtagc gcttatagat
    12181 tttacgctat aataacacat atttactaaa ctcttatgtt ctatagctat ggtctttggc
    12241 ctaccagttg accctgaagt agatatcgta tatgcacaat ttgatgatgt tattttttca
    12301 gttaattcat atccaaactt aaacatttct tcatcattta tatcgagtac tatggtattc
    12361 tcatttacaa ccttactttt gagttcatct gtagttatca ctactttcgc attactatct
    12421 tcaagaaggt aattcaatct ttcttctgga ttacttgtgt ctattggaaa aaatgctccc
    12481 cctgatttca taatccctat tgctgtaata attatgttaa tatctcttgg taacattaca
    12541 gctactatgt cttcactttc cactcctata tgatttaaat aacctgcaag tttatcagtt
    12601 acttcatcca attctttata tgttaacttt ctattttcat ccctagcagc tatattacta
    12661 ggtgttacct taacttgctc cctaaataaa tcaacataac ttttatcata gggtacttca
    12721 tattgattat tattaaagtc ttccaataat actttttctt cttccttaca tacaaattct
    12781 aaatcttcta ttaaaatatc attatcttct attatttgct ctattatata atcaaattga
    12841 tttaaaattc ttctaatagt ttctctttta tatatagatg gtttatactt tatttttatt
    12901 ttaagtgccc tcttagtata tactattaca gataaatcat atcctttttt ttctatgtca
    12961 taatcatatg ataaatcaaa acttttagct ataaatattg tgtttataag cttagtattt
    13021 agatgatttt tattctctat atctgataac aaatagtatc catactctaa agacctgctc
    13081 cactgccttt ttagcttcct tacaacatct ataaattttt cactttgtct aactcttact
    13141 cttattggaa ttatattcat tgttttacca aaaactacat catcttcata tatatacttt
    13201 tgaagtataa ggccatatat agtttctatg atagagtcta tgctaatatt gttcacttta
    13261 gaatattgaa ttaacctttc atgtttctca atattcatct taaaaacctc atctactact
    13321 tcatcagaac aatctttata aaaaaaatca tttggaattt ttgctatatt cgtataatca
    13381 tctaacaaat ccatccaata attcatagct cgttctaaat taacttcata catatcaaaa
    13441 ttccccctta ctactaagtc tatagaaaaa attcttttgg aattttattt tcacaatata
    13501 taaatatatt aataatacat atgcaagctc taaaaacatg agcttgcata aaattacact
    13561 tattatgact actaaattcg taatatttac aatgtttaaa atatattata acacatatat
    13621 gattttattt ttctacattt atataatata taaattacaa atagttattt aactaatata
    13681 taacaatacc ttttggaata tgatatatta tattaattaa atttttataa gataagttat
    13741 acttatagga gggaaccata taaatgaata tttatgctca tagggggttt agtggaaagt
    13801 atccagaaaa tactattcta gcttttaaaa aatgtttgga tatggatata tatggaatag
    13861 aacttgatgt ccacagaact aaggatggaa aaattgtagt tattcatgat gaaaaggttg
    13921 atagaacttt taatggacat ggttttgtaa aagactttac tttaagaaaa ttaaaaactc
    13981 taaactcatc ttttgaagga tatcaaagca ataaagaatg taaaattcca actttagaag
    14041 aagtattaat tctaatctca cctacagacc ttactttaaa tatcgaacta aaaactgaca
    14101 aaataaacta tcccaatata gaaaaaggcg ttttagagct tattttaaag tataatatga
    14161 aaaatagagt tcttatatct agtttcaaca gcaatagctt aaaaaacttt cataaattag
    14221 accctagtgt aaaaacagga ttattgtgtt atttacctat aaacaatgta gtcaattttg
    14281 ctaaatttct gggtaatagt tatcttcatc cacctctagt tttagtaaat gaatctctaa
    14341 ttgaactttg tcacaaaaat ctacttggtg tcaatgtata tactgtaaat gaagaggatg
    14401 atatactaca ctgtctaaaa ctcaatgtag atggtatttt tactaactat ccagatattg
    14461 cttcaaatct tttacactca aaacaataca gctaattata caatagtttg tactccttga
    14521 cttttatata attaatatgt acaattttat taaatttatc ttggaggtat aactatggat
    14581 aacttattaa atgaacttga agctttaaag agtaatttag ataatctacc aatagaaaaa
    14641 atagaaaatt atgatttgag taaaactgct ttatttataa ttgatgttaa taatggcttt
    14701 gcaagacaag gtgcattata ctctcctaga gtagagtctt taataaagcc aattgaaatg
    14761 tttacaaaaa aaatatcaaa caaattaaat agagtaatag catttactga ttctcatact
    14821 cctaaatcaa tagaactttt gagttatcct gttcactgtt tggaaaatga tgtagaaagt
    14881 gaattggttg atgaacttaa gtcgatagaa aacctacaaa ttcttcctaa aaattctact
    14941 aatggatttt ttgcattaga aaatttggat tttgataata tagataatat aattatagtt
    15001 ggcgattgca cagatatatg tatatatcaa tttgcaataa ctctaaaatc atattttaat
    15061 caacataata ttgaaaaaaa tatagttgta cctatgaatt tagtagatac ttatgatatt
    15121 cctaacgttc accctgctga gattttaaac ttagtatttt tcaacagtat gattcaaaat
    15181 ggagttaatg tattaaaaga gattcgttaa gcttctaata ctttacatcc cata
//
LOCUS       DAELNY010000084        15000 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.084, whole genome shotgun sequence.
ACCESSION   DAELNY010000084 DAELNY010000000
VERSION     DAELNY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.084"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..683)
                     /locus_tag="KPE55_002183"
     CDS             complement(<1..683)
                     /locus_tag="KPE55_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG0493246.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIE"
     gene            complement(676..1065)
                     /locus_tag="KPE55_002184"
     CDS             complement(676..1065)
                     /locus_tag="KPE55_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0493247.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            1257..1703
                     /locus_tag="KPE55_002185"
     CDS             1257..1703
                     /locus_tag="KPE55_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493248.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            1967..2692
                     /locus_tag="KPE55_002186"
     CDS             1967..2692
                     /locus_tag="KPE55_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493249.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            2679..3923
                     /locus_tag="KPE55_002187"
     CDS             2679..3923
                     /locus_tag="KPE55_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493250.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            4003..4680
                     /locus_tag="KPE55_002188"
     CDS             4003..4680
                     /locus_tag="KPE55_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493251.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            4681..7242
                     /locus_tag="KPE55_002189"
     CDS             4681..7242
                     /locus_tag="KPE55_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493252.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            7463..8737
                     /locus_tag="KPE55_002190"
     CDS             7463..8737
                     /locus_tag="KPE55_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0493253.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(8898..11939)
                     /locus_tag="KPE55_002191"
     CDS             complement(8898..11939)
                     /locus_tag="KPE55_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG0493254.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGET
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            12146..12445
                     /locus_tag="KPE55_002192"
     CDS             12146..12445
                     /locus_tag="KPE55_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG0493255.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(12683..14911)
                     /locus_tag="KPE55_002193"
     CDS             complement(12683..14911)
                     /locus_tag="KPE55_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG0493256.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         5712 a         1816 c         2215 g         5257 t
ORIGIN      
        1 tcttcaattc tatttgtatc tatcttaatc cttgacttta tggatccaat ggaagttata
       61 tataataaaa ccattaaaca aaagtcagta ccgattaatt taaatacatt aagaaatgat
      121 ttatctataa aaacatcagg agacccattt atgttccaat gagtggcaat tgagtctggc
      181 aattgattat aattaaatgc tataaaaata ctcattccca aagtaaatag tatcggtatt
      241 aaatagatta ttttaaattt ttttattatt ttatttttct tatttataaa atccatatct
      301 actattaatt ttgaatctat atctatatga cctaatttag aataaatttc tgatttaagc
      361 ttcttagctt ttttatgtgt atgaatatat agtatacttt catataataa aaaacctata
      421 ataccaataa aataggaaag ttccaattta ttaaatgtaa agaccagaat aaaaactaat
      481 ataaagtcta taataaaacc taaagataat agttttttaa attctttatc taactttata
      541 aagtaattat aatttttatg ctcttggtct atagaaactc catagatgtg ttttttacct
      601 acaaaatagg gcataaaata aaatgtagca tacatcaaaa ttagaactgg aaatattgta
      661 aatagacatt caatcttact cataatattt cacctcatac ttgtcaaata aacttgaact
      721 tagtgacaaa aattcttctt tactaatacc tgataaataa gattttgcca ccaataattc
      781 caagtcaagt ttattttttt gacgagttgt attatctatt ttcattggta gttgagctac
      841 aatagcacct tttcttctat caatatttaa gaatccctct tcttttaatt cattgtagga
      901 tttgttcact gtatgtaaat ttacaccaat attttcagcc atacttctta cagatggaag
      961 tgattcattt attttcaatt ctcctgaagc tatagcctta gttatttctt ctttaatttg
     1021 tacataaata gatttatcac tattaaaatc taaatttaaa accataaaaa acctccatta
     1081 tgctttataa atgaaaacga ttgttatata catagtataa cataaaaaat aataacaaac
     1141 acaaaatata agtttatatt ttgtaaaaat tgtaatttaa aaaataacat aatttttatt
     1201 aaaataatac agatactatt tatagataga aataaaaatt agaaaggaga aaaaatatgt
     1261 taacatattt aatgaaaaga caaatgcata aaaaagatga tggaatgagt aaaccaatga
     1321 tgattacagg agctgtaatt acaggtgtag gagcatatgc tgtatataaa atggtaaaaa
     1381 gccataaatc taattctcaa atggtcaata caaattatta tggaaatagc gattatgatg
     1441 attataaata tgacgaattt gattatgact gtgattgtag agacacaaaa caagattatg
     1501 aatatgaaga attaaaaaaa aaagtaaaag aatttaattc aagaagaata aattgtgaaa
     1561 attgtccaca tgtttctcaa gaagaaatga tgcaatatgt aaatagtgtt aaagatgtaa
     1621 aggaagagat tgatttacat gaagatgcta aaaaagataa tatttacaaa gaagatgaat
     1681 acaaaaaata tgaagaagaa taagtaatat tttgtataac tgaacaaata gtaatgtcaa
     1741 attatttaat atctagtttt aatatgtaag ttgtaactta acttgaaaga atcagtatca
     1801 ttctttagat agttaagtaa caacttacat tttattttta taaattttaa tagttctttg
     1861 tgactatttt gtaattttag aaatatttta gtatccaggg taattgtgta taataaaact
     1921 tgtatagata gatagtatga aattaagcag atagtttaaa attaaaatga aaagaaagga
     1981 aatagttatg ttaaagttgt tgatagttga agatgatagt actatatctt ttggaataaa
     2041 gtatgcttta gaacaagaag gatttagtat agatatatca aaggatttat ctagtggaaa
     2101 agaaatgatt tcttctaatg aatatagcat gatactgttg gatgttacat tgccagatgg
     2161 aactggttat gagctttgtc aatatataag aggattctct caggttccta taatattctt
     2221 gactgcttgt gatgaagaag taaatattgt aatgggtctc gatataggtg gagatgatta
     2281 tataacaaaa ccttttagaa ttagagaatt aatatccaga ataaaagctg ttttaagaag
     2341 aaaaggtaat acttcagaag aaaataagaa aatacttaaa tttggagatt taagtatata
     2401 tacattagaa gctagagtat ataaaaatga taaagaaata tttctgacat cagttgaata
     2461 taaacttctt ttaattctta tacagaataa aaatactgtt ttaagtagaa ctcaaatatt
     2521 ggaaaaactt tgggatgtaa cttatgattt tgtaaacgac aatactctaa ctgtttatat
     2581 aaaaaggctt agagaaaaaa taggtgatga tttatgtgaa ccaatctaca tagaaactgt
     2641 aagggggatt ggttataaat ggataggaag tgaaaatagt gtttctatat aatccagaag
     2701 tgaagaagtt tttaagtaag tatgttactt tgatgtttat tgttatcata atatctattg
     2761 gatttagcat aatcaacgtg tccttaacta aagagatggt tgtaagaaat aatcaggcta
     2821 taataggaac tttatcaagc aagtatccaa acttggaaag tcagatagta gacataataa
     2881 ctcaagggaa gtctatggag aacatagatt atggtcagaa aatattaatt aagtataatt
     2941 atgataaatc tattaggata aactctgagc ctattatttc taaattagtt ttagagacta
     3001 tcaaaataaa tataatatta gtatgtataa tatttatttt gatatttgtg ttggttgtta
     3061 gatattttaa gtcaatttac aatgatttat cagatatgac taagtatgtt tactatagtt
     3121 cagagggtaa gccatttgat atgaaaaaca agaatcaaga agggcagata ggtcttctaa
     3181 aaactgaact cttaaagatg acaacaattc ttaatgaaaa ggttgaactt ttaaaaagag
     3241 aaaagatatt tttaaacaat acaatttccg atatatcaca tcaattaaaa actcctatga
     3301 cttctttaat aatgttgaat gatttattat ataatgatgt accatacgaa gtaaaaatcg
     3361 attttttaaa taagataaaa aatcaattaa atagaatgga ttggttgatt aaaagcatgc
     3421 ttaaattatc aaaggtagaa gcaaaagtga taaattttaa aaaagataag gtaaaattta
     3481 gtgaactcat acatagagca atgctatcta tgaaaatacc tatggagatt aaaaatcaaa
     3541 aactgactat agagggaaat gataatgtat cttatattgg tgatattgat tggtccgtgg
     3601 aagcattagt gaatataatc aaaaattgtg tagaacatac tcctgaattt ggaaatataa
     3661 ttataaccta taaagaaaat ccattatttt ctgaactaat aataaaagat gagggtgaag
     3721 gtatagataa aaaagatata ccacatgtat ttaaacgatt ctatagagga agaagtagtt
     3781 caaaagaaga tagtgtagga ataggtcttg caatgtcaaa atctataata gaaagccaaa
     3841 atggcgatat atatgtaaac agcgaaaaag gtcagggtac agaatttcat ataatatttc
     3901 ataaaatgta tgatagtgac taatctgtaa tatttaattc actttatagt aatagccaaa
     3961 acctaatata tgaagtatag aatataaata ttggaggtag ttatggaaat tttaagagta
     4021 gaaaatctaa ctaaaagtta tggtaaaaat gaaacaaaag ttgatgctat aaaaaatgta
     4081 agtttatcag ttgaaaaagg aacgtttata gcaataacag gtccaagtgg aagtggtaaa
     4141 agtaccttat tacatttatt aggaggagtt gacagaccaa ctagtggtaa ggtttatata
     4201 aatgatgttg atatatataa tttaaaaact aaggatttgg cgatatttag aagaagaaat
     4261 ataggtctta tatatcaatt ttacaactta attccagtct taaatgtgaa ggaaaacata
     4321 ttgcttccag cagagcttga caatagagat attgatagag aatattttga tgatttaatg
     4381 aaaacattag gtctaattga tagagaaaag caccttccaa accaattatc aggaggtcaa
     4441 caacagagaa cctctatagg acgtgcatta attaatagac cttctataat attagcagat
     4501 gagccaacag gtaatttaga tagtaaaaat tcaaaggaaa tattagaatt gttaaaattg
     4561 tcagtaaaaa aatacaacca gacactaata atgataactc atgacaaaaa tatggcactt
     4621 caagctgata gaattataag catagaagat ggaatcataa aaagtgatga ggtgatgtag
     4681 atgaatttat atacattact aaccattaga tacttaaaac aaaataaaag aagaaccatt
     4741 gtaaccataa tcggtataat actttcaaca gctttaattt gtggtatagg aaatatattt
     4801 gaaagtttta tggattatca aatgagagaa actataaaga atgatggaag tttccatgtt
     4861 acattttatg atgtaaatag aaaaaatgtg gaatatgtaa cgaaatcagc tgaaatagaa
     4921 aaacatgcat ttaccaaaca acttggatat tctaaattag aaaatagtga aaatgctata
     4981 ttaagtataa agcaatatga taaaaatgca ataaatggat ataaagtttc tataaaagaa
     5041 ggtagatttc cagctaagga aggagaaatt gttctaagtg aaagtattat taatcttata
     5101 gatgataagt taaaaatagg agataagata actttaaaag taggggatat gtttgactca
     5161 agcaagaaaa agattgatag tatgacattt catgaaggtg attatatagc aaatgaaaaa
     5221 gatagaactt ttaaagttgt tggtataatt gaaaaacctg ggtttgaacg ttataatgga
     5281 attgcaacag ctatagccta ctttaatcct atgaataatt acaatgatac aattaatgta
     5341 tcagtagcag taagaaatcc taaagatgtg tataagataa gtaataaaat aagtaaaaat
     5401 atatattcaa ataaagataa agaagcagtt tctgatatga taaaatacaa tgaacattta
     5461 ttgaggttac aaggtgctag taagtattca aatataaata actctataaa gtctataatt
     5521 atagcagtaa ctattttagt aatcatatgt acaattgcta cagtgtacaa ttcctttagt
     5581 atttctataa atgaacgaaa aaaacagttt gggatattaa attctatagg tgctacatct
     5641 agtcaaatta aaagattagt ttttatagaa ggtattataa ttagtttaat tggtatacca
     5701 ataggactaa tatctggaac agtagctata gacctattgt ttaaaattat aaataagtat
     5761 tttactgaat cggttgttac taagatgagt ttgcagatag tgtataatcc aataataact
     5821 attgttagta taataatagt tttgcttaca atctttatat caattctact acctgcaata
     5881 tcggcttcta atatttctca ccttaatgtg attaaaaata ctggagagta taaagtgggc
     5941 aaaatcaaaa gctcaagact tattaaaatg atttttaaaa ctgaaggagt acttgcatat
     6001 aaaaatatta ggagaaatag aaaaaaattt ataataactc ttttttcttt aatggtaagt
     6061 ataataatat ttatatcttt tagtggcttt acattacttt tactaagagg tgaagaaatt
     6121 agaaattctc aaagaaaata tgatttgtat ttaactgcaa agaacacagc taggtctgta
     6181 gaggatacta taagtgaact agaagatatt gatgggataa aaaactttga attggcaaca
     6241 ggacaatatg tgtctataag agtaagtgaa aataaaataa ataaatccaa ggaagattta
     6301 atcaggaaat attatcaaaa atataagatt ggagatagtt atgaatatga tttcagtaat
     6361 aatgaattaa tttttccagg agattttgca gtaaaaaaca aaataaataa cttagtacag
     6421 ggctctttta ataaagagag agcaattgaa gaaaatggtg ttatattagt tagaaaaagt
     6481 gcatttgaag aaaatgggaa gaaaggtgta gtagaactta ccaactataa agtaggagat
     6541 acagtaaatt gtgaatatct tgatgaaaat gccttaagta aaaaagtaaa ggtcaagatt
     6601 cttgctataa ctgacgaaga acgtctaggt cttggttatc aaaatatggg gttacaattt
     6661 ataacttatg atgaagttgc taaaaattta aatttaaaat taagtaaaag ccttatattt
     6721 attgattcag gaggaagcat taagactaag aaggaaatag aagctttggc aaataaaaat
     6781 aattttaact tttatgatga aagtgcaagc aatgaaagtg aaaaacaaga tttaaaggtt
     6841 attaaaatat ttgtatatgg ttttatagta gtgatatctt tagtgagtgt tacaaatatt
     6901 ctaaatacag taagtacaag tataaatcta agaaaaagag aacttgcagt aatacaatct
     6961 ataggggtta ctcctaaagg atttaggaaa atgatatatt tagaaagttt tatatatggt
     7021 atactgtcat tgttgtttgg aataccaatt agtattggta taattctaat tatgaataaa
     7081 cttatttcag gtgtaattga attttctccg attataccgt ggacagccat agttatctgt
     7141 atagtaagtg tttttataat aacttttata gcagggtata taccaatgag taaattaaat
     7201 aaagaaaata taatagataa tataagaaga gaaagtatat agatataaaa ataattctta
     7261 actatataaa atattattaa aatcatttaa gtggtataaa atattattat atcacttaaa
     7321 tgatttttta tttatataag aaatctaata ataaattact agaggtattt aactatttag
     7381 aaagaattgg tattatataa agtaagagaa tatataataa gaaagaatat ttgattttat
     7441 tggaaaacaa aagaaaaaga aggtgattac tatttctcat ataactgcga agaatatgta
     7501 taaaagttta gaggaaagaa taaacaagtt tccacaaggt gctccaccat ctgatacatt
     7561 atacaaaata ctaaatgtac tatatacaga acaagaagcg aagttagttg ctcagcttcc
     7621 aataaagcct ttcagagtaa aaacagcagc taaaatttgg agtgtaagtg aaagtgaagc
     7681 atatagagta ttggataaac ttgctagtaa ggctctgata ctagatatag aagataataa
     7741 agggaagaaa tatataatgc ctcctccaat ggctggtttt tttgagtttg ccatgatgag
     7801 aacgagacac gatatagacc aaaaactttt ggcagagctt tactatcaat atatgaatgt
     7861 agaagaagat tttatcaaag atttatttta ttcaacagaa acgaagcttg gaagagtata
     7921 cgttcaagag gaagttttaa ctaatgataa tgaggttagt attttagatt atgaaagggc
     7981 tactcatata attgatgaat caactcatat aggtataagt atgtgttact gtagacatag
     8041 aatgcaacat gttggaaaag cttgtgatgc tcctatggat atatgtatga cgtttgacaa
     8101 tgtagcaaat tctttaataa ataataaatt tgcaagacgt gtggataaaa tagaatgtaa
     8161 agaactactt catcaagcat atgaacataa cttagttcaa tgtggagaaa atgttaggaa
     8221 aggggttacg tttatatgta attgctgtgg atgttgctgt gaagcaatgg tagcagctaa
     8281 aagatttgga aatcttcatc cagtacagac aacaagtttt ataccaaata taaaccatga
     8341 aaattgtgta aagtgtggta aatgtataac tgcatgtcca atagatgcga taagcaaggt
     8401 taaggaagat ggtaaagaat atataaaaat tgatgaggat agatgtctag gttgtggagt
     8461 ttgtgttaga aattgccata aaaatagcat tatgttatta aagagagatg aaaaaataat
     8521 aactccagca aattcagtac atagagcagt tcttatggca attgaaaaag ggcagttaca
     8581 aaatttaatt tttgataata atgctctggc tagtcataga gctatgggag ctatactgtc
     8641 agctatatta aagcttgaac cagctaaaaa gattttagcc agtaaacaat taaagtcagt
     8701 atatttagat aaattgttaa gtatgaatga taaatagaaa ctaacttaaa aaaggcaaag
     8761 ttttagactt atctatatct taataaactt atcatttaat atttaaattt aatttttaca
     8821 catataaaac ttataactta aataaaaaaa ggctatattc aattaatatt acttgaatac
     8881 agcctttttt agttatgtta agctatttct tttatttgct taaatgagtt ttcatttcca
     8941 tttccaccaa tttgtgttac agatttaaat ctcatagttt taaatagttc tttttgatta
     9001 taatctagtg agtttcctac tagcattaca ggagatttag tttttccagc caaaactcca
     9061 acagataatc cgtctattaa atcatcttgt ttattcattc catttttagc aacatataaa
     9121 ttgtctatct tagaatcctt gtaaaattct tttattactt ttgcattagt ttcatttcta
     9181 gtgctacctg atattctctg tggattttga agtttacttt ctatattttt agaaactgta
     9241 tattctccac cgattacata agatttatca atctttttgt ttttaaacaa gttatttatt
     9301 tcagttatat tactattttc attagtaagt attataggca tatcattttg agctgataca
     9361 gctccaatgc ttactgcatc ggctaatcct ttttcgccat taactactga aatctttgat
     9421 atattgctaa tattgttcaa ttctttagca atgttcatag aagtttgata tctatcactt
     9481 cctgaaattc tagatacaga gatattgttt gattttatag tatctaaaga tttttcattt
     9541 atagaagttt ctccaccaac tacatataca tttttaactt ttaatctctt taattcagcc
     9601 aaagtttttt catttatttg agagcttcca gtcaataata taggagcgtt tttcttgtat
     9661 gcaaatggtg ttgcagccaa agcatctgca attgctgaat cgtttattaa aactgcattt
     9721 tcagcatttt cccaacctgc ttgacttatc ttaatagaag tttcatatct atcagttcct
     9781 gtaagtgcac ttgcatttac ttcatcttga tatgtaacct caaatctagc atttggactt
     9841 cctaagaaaa caattacgcc tccttttggc aatctaactc tattgttctt tgtaactaaa
     9901 gaatagttta caggaacacc tttatcatca taaacagcaa aagctgaatt ttgaggaaga
     9961 ttaacttcta tttttttatt tgcgatatcg tcaccaatct tataccattt tgcataacca
    10021 ttggattcta gttcacaagt aaaagatttt tctgctggca aattagttat agaatcttca
    10081 ctaacatacg ttagtgttcc aaaagataaa tactctgtac cattttcttt atgaagttta
    10141 atgtcggata aatcacgacc tattacacca ggtatttcta taaaagcatt tgagttattc
    10201 tcatccatta ttttagtgtt aactatataa cctggagttt catcagaaag actaaaactt
    10261 gcttttacag agccaaacat atagctttga gaagtatatt tttcatccac tagatagtaa
    10321 cctttgccac ttcttttttt ccacacttct ttgacactat ttgatatatt gttgtcatct
    10381 actttttgag ctacatagta taaacttgct gtctgaccta atccagggac atcatcataa
    10441 gagctcatat ttaaatatgt gatattattt ttttctttta caaattttaa acaactatta
    10501 cctttttcag atacaaatct atcttgacct atgtatacgt atttatcttc aggtccattt
    10561 tctatataag gagaagatac tgttaaagta ccattatcat taacatctac ttttatcata
    10621 tttgaagaag cataaagacc actattttct tttaaagagc taggcatttt aacttgttgt
    10681 ggcttactaa atgttttatc aggttttatt tctttaattt tacctttttc ttttaatgca
    10741 gatagtaaga tttcttgtcc aattatttca ttaagttgac tacttccacc agaagaaaga
    10801 actgcaactg ccatattttc atctggaaga actataagat tactgtggaa taataaagaa
    10861 tctccacctt ttgtaagagc ttttaaatta tactgattaa atggataagt gttgacacaa
    10921 tcccacccta aaccatatcc aagtatagag tcttctcctt caggccatag accatttaaa
    10981 tattctttgt tttccatagc ttttactgaa gcaggagaaa gaataccatt tgaatttttc
    11041 ataaatgttt gtgcaaatgt acatagattt tctgcacttg aatataaacc acctgcacct
    11101 atagcattaa ggttatcttg tggaactgca tcttcccaat aaggtacata tgcttttgct
    11161 agttttgaac tatcaaaact attttctgga gtttttgtat tttgtaagtt aagtggatta
    11221 tttatgtatt tatcaagaaa attagtaaat gacataccag atactctttc tactaaaatt
    11281 tcagctaaag taaatccatc attacaataa acagaaaaag ctcctggttt agcttttaat
    11341 ctttgttttt gtaattcttt taagaaatta tcatgaccat aagaatcatt gtctgctaat
    11401 aaaatagtgt ttttaaaact acttcccatc aaccctgatg aatgatttaa taacattctt
    11461 ggagtgattt ccttgtatct atcatcagcc attttaaatt caggtatata ctttacaaca
    11521 ggagtatcta aatttaattt tccatcatca accaacttca taacagctgt tgtagtaaac
    11581 attttactga tagatgcgat actatacata ttatccttat ttaagttttt gttatcctgt
    11641 ttgctgtaaa cgccaccatt accagaaatc tcaattttgc cattatcaat caaagcatat
    11701 tgagcactta cagcaccata ctttgatagc aaagtttgga ttttctcttt agcaagagct
    11761 ttagtttttg tgtaagattc cgcgttttta ctataatcat gtaattctat atcagaactt
    11821 tgatattttt cggaattatc ttttgtatta tcagcaaaag taatatttgg agcagcagta
    11881 aataacatac tcaatactaa tgtactagat aataatttag agagcctttg cttaaacata
    11941 ttacattacc tctttccctt tttaataatt tataattagc ataacattct taattatttt
    12001 aatcaacaga ttattatcaa taaaaaaagc ttttataagt atagcttttt atataacaga
    12061 gaaaaatata aaaataatat ttgttgttgt ataaagaata aagggtatag gtaatatata
    12121 aataaaatgg aggaatttta gacatatgta taagaatata aataagaagc aacttaaaga
    12181 aatgatgaaa aatgaaaagg atgttttatt attagatgta agaactaaag atgaatttaa
    12241 ggaatataaa atagaaaatg ctataaatat atctttacaa gagttaatca ataatataga
    12301 tgaaatttac gattataaag ataagaaagt agtagtttat tgcagaagtg gtcatagaag
    12361 tgttactgca tgtaatttgt tagctgaaga aggttttgag catttatata atttaaactc
    12421 aggaataatt gattatatga gttaaataat gtaaattata cattaaatac tatgtatagc
    12481 tctgcttatt aagtaggaat atatctaaat atttataaga taaatttagg aaagaacgaa
    12541 ttttttcgtt tttttctatg acagtttttg tagtaaatat tagtttttaa gttggaaaaa
    12601 ggtagtagct ttttagctac taccttttaa tattaactta gaatattatt aaaaaaatat
    12661 atttaaagac agaattaatt ttttaatgac cagttaaatt actatcgcca aatttatcat
    12721 taaatggttt tataactcta tttataggtc cttttagaag tacgtttaaa caaatagaca
    12781 gaatcataaa tagtaataga actagtatat ctttagttag atttggagca tatataccac
    12841 caactgcttc ccttgtagca cctattgcat aggtaaatgg cattataggg tttattcttt
    12901 ggaagaatgt aggtgtaact tgtataggga atgtaccacc agaagaacca atctgaacga
    12961 ctaatagtac tattgcaatt gcttttccta catttccaaa tacagaaact aaagaataca
    13021 ctataaagtt aaataccata ctagtaaaca taagcagaac tatcaataaa attggatgtt
    13081 ttgctgttac tcctaacaat aatatatctc cagttcctat tataaaacct tggacaagag
    13141 ctatacttaa aaaagtaagg cctcttccaa agtaaatttc ataaggttta tagtttccat
    13201 gtacagatgt agacaatagt gctgaaataa gtaatatacc aacccatgta gaaagaacac
    13261 tataaaatgg tgtcattgca gaaccataat tttcaattgg atataatttc tctgttttca
    13321 cctcaacagg ttcttttaag aaattagact ttgtcaatat atcactttca agcaagctga
    13381 ctaatttttt catatcttca ccattactaa ttttgcttaa agtatcaatc aaatcatcca
    13441 acattccttt tgctaaagga agtctttctc ttatcaatga tatactttcc tgtgcatttc
    13501 ctgaaagttt tagagaagta gtaagtattt cttcaacctt tggaagtttt gcttctgcct
    13561 tatctaaaac agtaattata tcatttgcta cttttatact gtttgcaaat atactattta
    13621 taggttttga tatttttgag tcaaaattat ttaaaatatt taaattaatt cttccaatac
    13681 catttgatag acttaaaaca ttattcaaag cacttataga tggttgttgt ccagatataa
    13741 ctttgttttt aatatcattt aatgttgata tagatgaatc tattttattg ctagagtctt
    13801 caagattatc tatgacatca tccaatctat tatttgatgt aagttggttt aatttagtta
    13861 aaaaatccac caaagtatca tttaaagatt gcaaattaga aagtttttct gataaattat
    13921 ctatcaactt aggtacatct tcactgccac tatttactgc atcaattaaa tttaatgtaa
    13981 gagaagaagc agaagatgaa aggtcaacca ttaaatttaa gtcagactta ataagaggag
    14041 aaagttcgtc taaattatca tttgtatcct ctaagaattt ttttaagtca cttgataata
    14101 gttttgtatc attcagagtt tttttaattg taggtaaatc agatttaata tctttaactg
    14161 tatcagataa ttttgaagta gcatcagaag ctaaatcaac agtcttattt atatggtcaa
    14221 atctcttttg aaggtcaata agggaatttt caacttttgt caattttgga agactgtgtt
    14281 caattcctgc accaactccg tttgatatac caagaacgat ttcactaact gtttttacta
    14341 tagtctgatt aacctgtagt tgtattgtag aagcaccttt atctgtaatc tttggtgcaa
    14401 ctgcatttat tttttcatta acagtataga taatttcgcc ttttttaaca tcatctgtaa
    14461 ttaaagatgt caaatctttt gtaaaatttt ttggaatctt aagtgatgca taataagtac
    14521 cttttttaac accttcaaga gcagtttttt catctacaaa ttgccaacct aaactcttgt
    14581 tatctttgag ttgttcaatt aattggtctc ctacattaat cttttgccct gctatttcat
    14641 ttcctttatc ttcatttgtt actgctacaa gcatattggc agtgcttcca taagggtccc
    14701 aagatgcctt tatattgaac caagcataca gagatggtaa aaaagcaaga cctaaaacta
    14761 ttacaagtac ggcataattg ccacgtatat cttttaggtc acttttaaaa atttctaata
    14821 tattttttaa accctttgaa ggtcttaaat tcttgatatt attccagttt aaaatttctt
    14881 ttaaactatt agtttttttc aaatgtctca cctcctaaaa tattttgata aagaaaaaag
    14941 ttagatttat aatcaatttt aaattatatt actttttaca caaatagtaa agttaaaatt
//
LOCUS       DAELNY010000085        14922 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.085, whole genome shotgun sequence.
ACCESSION   DAELNY010000085 DAELNY010000000
VERSION     DAELNY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.085"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            204..359
                     /locus_tag="KPE55_002194"
     CDS             204..359
                     /locus_tag="KPE55_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493257.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            489..1502
                     /gene="aroF"
                     /locus_tag="KPE55_002195"
     CDS             489..1502
                     /gene="aroF"
                     /locus_tag="KPE55_002195"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0493258.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1524..2588
                     /locus_tag="KPE55_002196"
     CDS             1524..2588
                     /locus_tag="KPE55_002196"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG0493259.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFDKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2588..3901
                     /gene="aroA"
                     /locus_tag="KPE55_002197"
     CDS             2588..3901
                     /gene="aroA"
                     /locus_tag="KPE55_002197"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG0493260.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGIKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3879..4958
                     /gene="aroC"
                     /locus_tag="KPE55_002198"
     CDS             3879..4958
                     /gene="aroC"
                     /locus_tag="KPE55_002198"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG0493261.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4961..6157
                     /gene="pheA"
                     /locus_tag="KPE55_002199"
     CDS             4961..6157
                     /gene="pheA"
                     /locus_tag="KPE55_002199"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG0493262.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFSGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6204..7013
                     /gene="aroE"
                     /locus_tag="KPE55_002200"
     CDS             6204..7013
                     /gene="aroE"
                     /locus_tag="KPE55_002200"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0493263.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            7025..7558
                     /locus_tag="KPE55_002201"
     CDS             7025..7558
                     /locus_tag="KPE55_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG0493264.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7579..8418
                     /locus_tag="KPE55_002202"
     CDS             7579..8418
                     /locus_tag="KPE55_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG0493265.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKSK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8686..10167
                     /locus_tag="KPE55_002203"
     CDS             8686..10167
                     /locus_tag="KPE55_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0493266.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     SFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10200..13637
                     /locus_tag="KPE55_002204"
     CDS             10200..13637
                     /locus_tag="KPE55_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0493267.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFINNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13655..14401
                     /locus_tag="KPE55_002205"
     CDS             13655..14401
                     /locus_tag="KPE55_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG0493268.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPQNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14350..14658)
                     /locus_tag="KPE55_002206"
     CDS             complement(14350..14658)
                     /locus_tag="KPE55_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0493269.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         6179 a         1464 c         2486 g         4793 t
ORIGIN      
        1 atatttaaaa taaaaaaatt attgtaaatt acagatgatg tttaattgat tttataaaaa
       61 tacatcaatt gagaagctga tttaagtaaa taaaaaaata ttttaaattg aaaatattta
      121 tagaaaatat tgacaattat attttctttg ttataattat taaacaagaa gaataataaa
      181 ttataaaaat ggaggataat actatgaaag gaatattaaa agaactaaca gcaaaacttt
      241 atagcttttt ttactttatg ttctggggct tttattttag cgctggctat ttcttttaca
      301 aaaatatatg tacttccttt ctggtgaggc cttattttag tgattttgag cataagtaaa
      361 ttttataaaa atggttattt tatatattta tataagattt agtgcttgat agattatttt
      421 agagaactca ttagtgagtt ctctttttta ttttaaatat aaaattataa tattgggagg
      481 tagtaaaaat gatagtagta ttaaaaatgg gagcagacaa aaatgaagtt aaaaaattaa
      541 ttgaggctat tgggagagaa ggagtagaag ttaatcccat agatggaaca gaattaactg
      601 ttttaggttt agttggagat actagtaaaa tagatgccaa aagaatagaa gcaaataagg
      661 tagttgaaaa agttatgcat gtggtagagc cttttaaaaa ggccaataga aaatttcatc
      721 cagaaccttc aatcataaat gttaatggaa tggaaattgg ttcaaagaaa attgctatga
      781 tagctgggcc ttgttctgtt gaaacagaag accaaatagt atctatagct aaagatgtga
      841 aaaaatcagg tgcaggattt ttgagaggtg gagcattcaa accaagaaca tcaccatacg
      901 ctttccaagg attgaaatat gatggattgg atttattaaa aaaagcaaaa gaaaaaacag
      961 gacttcctat agttacagaa ataatgtcaa cacaagatat agatatattt gaagaaaatg
     1021 tagatgtaat tcaagttgga gctagaaaca tgcaaaactt tgatttatta aaagaattag
     1081 gtaaaacaaa taaaacaata cttttaaaaa gaggattatc tgctacaatt gaagagtggc
     1141 taatgtcagc agagtatata atggctggag gaaatgaaaa tgtagttctt tgtgaaagag
     1201 gtataagaac ttttgaaaca tatactagaa atactttgga tttaagtgca attttagctg
     1261 tgaagaaact tagtcattta ccagttatag ttgacccaag tcatgcagca ggtaaatcat
     1321 ggatggttga ctctttatct aaggcagcaa ttgcagttgg agcagatggt ctaataatag
     1381 aagttcataa tgacccagca catgcacttt gtgatggaaa acaatcgatt aagccaaatg
     1441 aatatgatga attgataagt gaattaaaga ctatcgcatc agcagttggt agagaaatat
     1501 aatctataaa aggatggtta aacgtggaag aaaaactatt agtaaattta aaagaaaata
     1561 gctacaatat atttattaaa aaaaatatac taaagaatat aggagaagaa ataaaaaaaa
     1621 tatattttgg aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa ttatacttgg
     1681 ataaagtaaa aaataattta agtgaatctg gatttgaaat taaatcttta atattaaaat
     1741 ctggtgaaga aacaaaatca tttaatactc ttcctaaaat atacgatagt ttacttgact
     1801 ttaagttaac tagaaaagat ttgattatta ctttaggagg aggggtaata ggtgatttag
     1861 gtggatttgc agcagcaact tttttaagag gtataagttt tattcaaatt ccaacatctt
     1921 tattagcaca agttgatagt tctgtaggtg gaaaagttgg agttgacctt gagagaggca
     1981 aaaatttagt gggaagcttt taccaaccaa aggctgtgtt tatcgaccca aatgtattat
     2041 tatcacttcc agaaaaattc tatagagatg gaatggcaga agttataaaa tatggatgta
     2101 taaaagacaa aacttttttc gataaacttt taagtttaaa aaatagagaa gaagtaatgc
     2161 aacatataga atacattata cataaatgtt gctttattaa aaaaactgta gttgaagctg
     2221 atgaaaaaga ctttggagat agaatgcttc taaactttgg ccatacatta ggtcatgcta
     2281 ttgagaaata ctacaatttt acgggttata cccatggaga agctgttgcc ataggaatgt
     2341 ataagataac tttagaaagt gaaaagatag gaattacaaa aaaagggaca tcagaactta
     2401 taaaatcaat tttaatgaat tataatttgc cttatgagat tgaattaaga gataataaat
     2461 ctatagtaga tgctatatct cttgataaaa aaaatctagg tgatacatta aaaataatac
     2521 ttttaaaaga tattggagag agtattatat ataatactac ttctaaattt tttcaggagg
     2581 taagataatg ggaagtctaa aaatatatcc aagtaaatta tctggagatg taaaaatacc
     2641 accatcaaaa agtatggcac atagggcagt tatttgttca tctctaagca atggaaaaag
     2701 tagaatttct aatatagatt tttctgatga tataatagct actataagag caatgacatc
     2761 attaggagct attatagaaa agaaagaaga tattttagaa atttcgggga ttttttctaa
     2821 agaaggtatt ttaaatagag agaatcagtt gaatcaacct aaattgacaa tagattgtaa
     2881 tgaatcaggc tctacattga gatttttagt acctatatct ttagcatttg atggtataaa
     2941 aaggtttata ggtagaggta atcttggaaa aagaccactt gatacttatt atgagatatt
     3001 tgacagacag aatataaaat atagttacaa agaaaatcag ttagatttga ttatatcagg
     3061 taagcttaaa ccagatgaat ttagagtaaa aggaaatata agttctcagt ttatcacagg
     3121 tctactgttt atattgccta cgctagagag tgattcaaag ataataatta ctactgagct
     3181 agaatctaaa ggatatttag atttaactct ttcaacgata aaagattttg gggtagaaat
     3241 tataaacaat aactataaag aatttattat aaaaggaaat caaacgtata aagcgagaga
     3301 ttataaagta gaaggagatt attctcaagg agcattttat ttatcagcag atgctatagg
     3361 agaagatatt agcatattag accttaaaga agattctctt caaggggata gtgaagtagt
     3421 tgaaatttta tctaggatgg gaatggaaat attaagagaa ggcaacaaaa taaaaggaat
     3481 aacaaatggt ttaaatggta ctttgataga tgcatctcaa tgcccagata taatacctgt
     3541 attatctgta gtagcatcct taagtatagg gaggactaca ataattaatg ctggtaggct
     3601 tagaattaaa gaatgtgata gacttcatgc aataaatgtt gagttaagta aacttggagc
     3661 aaatatagag gagaaagagg atagtttgat aatcgaagga gtaagcaagt taaatggtgg
     3721 agtagaggtg tggagtcaca aagaccatag aattgctatg acacttgcaa ttgcttcttg
     3781 tagatgtgat aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat cttatccaca
     3841 tttttttaaa gattttaaga tgttaggagg tcgaattgat gagtggaata tggggaaata
     3901 atttaaaagt atcaattttt ggagaatctc atggaaatgc tataggtata aatatagatg
     3961 gtcttccttc ggggattgaa cttgaccttg ataatattga taaagaaatg aagagaagag
     4021 cacctggaaa aaattctata tctacttcaa gaaatgaaag tgatatacca gaaattctaa
     4081 gtggatattt taatggaaga actacaggaa cgccattatg tgcaatcata agaaatagtg
     4141 atactcgttc aaaagattat ggagagctta aaaatttaat gagaccagga catgcagatt
     4201 tcacaggtaa tgtgagatat tctggattta atgactatag agggggaggt catttttcag
     4261 gtaggataac agctccatta gtattttgtg gggctatatg taaacaaatt ttatcacaaa
     4321 aaggaataga gataggagca cacataaaaa aaattaaaaa tatagaagat atgagttttg
     4381 attatgtaaa tatttcaaaa caacagttat caaatttgca aactttggaa ctacctttac
     4441 ttgatttatc aaaggaagaa gccatgaaaa atacgattat agatgcaaaa aatcaaggag
     4501 attcagttgg tggtataatt gagtgtgcag tagtaggtat taatgtaggg cttggaaatc
     4561 cattttttga ctctgttgaa tctactttat cacatttatt gttttctgtt cctgctgtaa
     4621 aaggtgtaga gtttggttta ggttttgaac ttgcagatat gtatgggtct caaagtaatg
     4681 atgaaatgta ttatgaagga aatcaagtta agagcaaaac aaataacaat ggaggaatta
     4741 taggagggat tactactgga atgccaataa ttttcaaagt ggcaattaag ccaactccat
     4801 caattagcag acaacaaaac acagttaata taaaagataa aaaggacgat attttatata
     4861 ttaaaggtag acatgaccct tgtatagttc aaagggctat acctgtaata gaagcagtaa
     4921 cagccattgg aatttttgat ttgatgaaag gacgataata atggaggatt taacgagata
     4981 tagaaatgag attgacaata tagataaaga gcttattcaa ttatttgaaa agagaatgaa
     5041 tacagttctt gaaattgcca gacataaaat gaaaaataac actactatac ttcaaaaaga
     5101 tagagaagaa aaagtactaa gtagagctgt agataattta gataataaag catattcaca
     5161 agaaacggta cagtttttta attctataat ggaaattagt agaggattac aaaaaagatt
     5221 gatagataat gatggtgagc agaaaatcga gtttagttta gattcatcaa ataagaaatt
     5281 tgatttgtca aatgtaaata gatataagtc tttaaaagat gaattaaata aaaaaaatat
     5341 cctagtaggg ttttcaggaa aatcaggttc atttacagaa gaagcactaa ataaattctt
     5401 caacaaaaaa atatcaaaaa aacaatttaa agaatttgaa gatgttttca tagcacttaa
     5461 aaataaagaa ataaactatg gaataatccc catagaaaat tcatctacag gagctatctc
     5521 tgaaacttat gaccttttga gaaagtacgg tttttatata gttggagaag aatgtataaa
     5581 aatagaccaa aatttaatag ggataaaaga cacaaagctt gaagatataa aagaaatata
     5641 ttctcatcca caaggtatag gacaatgtag tgaattttta aaacaaaata gtacatggaa
     5701 attgatacca tttaataaca cagcaacaag tgcagaactt gtaaggaaac ttcaagataa
     5761 aacaaaagca gcaattgcaa gcaaaaaagc tgcaagtatt tatggattag aaataataag
     5821 tccttgtata aatgatattg caaacaacta tacaagattt gtagtaatca gtaatcaaat
     5881 acacattgaa gaagaatcaa acaaaatgag tgtagtattt tctgttgaac atgaagcagg
     5941 aaaactttat aaagtacttg gatactttgc taaaaataat ataaatatga caaaaataga
     6001 atcaagacct atgaaaaatg cctcatggag atatttcttt tatatagatt ttgagtgttc
     6061 tatatacaat tctaaagtgt acgatttatt agaattgata gaacataata cagcatattt
     6121 taaatttatg ggagtatata aaaataaaac tgaatagata agataaattt ttatttaatt
     6181 tataattatt aggaggtgta agtatgaatt ttttcggtct agtaggtgaa aaactctctc
     6241 acagtgtttc acctcaaatc cacaagagag tattcgagat attgaatatt gaaagtgctt
     6301 ataaaaactt tgaaatttca aaagaagata tatctaagct agacggtgct ataaaattat
     6361 taggaataca aggagtaaat gttactgtac catataagga gcgtattatg aaatatcttg
     6421 attttatatc accagaggca aaacgtatag gagcagttaa tacaatttta ctcagagaaa
     6481 atatgttata tggttacaat acagattact ttggtcttga tagtatgttt aaaatggcta
     6541 atattgatgt tcaaggaaaa gtagcagtta tattaggaac tggaggtgct tctaaagctg
     6601 ctcttacata ttttattgat tctggtatag aaaagcttta tgtttcaact agaaaaaagg
     6661 atgataaaaa attattaaat agtaaagcta tattaataga ttatgaagaa cttaaacata
     6721 taaaaggaga tataatatta aatgctacac cagtaggtat gtaccctaat gtaggaattt
     6781 caccagtgtc aaaatctatt atacaaaatt ttgatatatt aatagattta atttacaatc
     6841 ctggagaaac agagttttta agaattggta atagtatggg taaaaaaact tgtgatggat
     6901 tatatatgct ggtggggcaa gcaataaaat ctcaggaaat ttggcaagat actaaaatag
     6961 ataatagtat tttagatgtt atatataatg aactaaaatt agaatttttg taagggagat
     7021 tgttatgata aataaaacaa aagaaaaatt gatattaata ggtatgccag gtagtggaaa
     7081 aactacaatt ggtaaacttt tggccaaaga atataattgt agtttctgtg atatggatga
     7141 ttatataatt caaatatctc aaaaaagtat agcagagttg ttttcagaag gagaagatat
     7201 ctttaggaac tatgagactc aagcatgtag agaactttct ataagtgata aaacagtgat
     7261 atctacaggt ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa aagaaacagg
     7321 tataataatt tttattgata gacctataca aaagattttg gaagatataa atattaattc
     7381 aagaccatta ttaaaaaacg gaaaagatag attgtataat ctttacaatg agagaataaa
     7441 tctttataaa aaattttcag atatagagat tttaaatgat aaatcattaa ataatgctgt
     7501 atataatatt acaaatgcag tcagtgaaaa ttttaaattt gactttaaag aaaaataatc
     7561 tagggggaag taaaaataat gaatattgtt attgtaggac taggagttat aggaggctct
     7621 tttgctaaag cattaaaaaa agctggatat gaaaatgtat ttggagtaga tgtagattta
     7681 gaaactttaa aaaaagcaga aaaatcaaaa atcataaaaa aaggatgtac tacaggtaaa
     7741 gagttattta aaaaagctga tttaattata ctatctatat atcctagatt agttgtagat
     7801 tttctaaata ataataaaaa cttttttaaa aaagggacta taattactga tactacagga
     7861 ataaaagaaa cacttatcaa tgatgtgttg caaattattc cagatgatat agattttata
     7921 tttggacatc caatggcagg tagggaaaaa aagggtattg attttgcaag tgaacaagtt
     7981 tttaatggcg caaattatat aattactcca actggcagaa acaacataaa aaatttagag
     8041 ttagttgaaa atttaatttt agagattgga tttaaaagag taaaaaagtt aacttcacaa
     8101 aaacatgatg agattatagc ttttacttct cagttacctc atgttatggc agttgctctt
     8161 ataaatagtg atgaagaagg tagagatacg ggtaaattta ttggagatag ttatagagat
     8221 ttaactagaa ttgcaaatat gaatgaagac ttatggagtg aacttttttt aggaaataga
     8281 gataatttac taaaagttat agaaaacttt gaatcagaag taaatttagt aaaagaagct
     8341 atatttaaca atgataaaaa taaattaata gagtatttta aaaaatcatc aataagaaga
     8401 gaagctttag aaaaataaat attaatattt aagctatata tagatgggta taaatataag
     8461 cgatagtgta tttaattgta ttaataaata tggattcaat ataattaaat atgctattga
     8521 tatatttgta cccttaacta ttgttatctt ttaaatgata atgatattta tttaaatact
     8581 ctttattaac aatttataaa acttaattgt aaaaaatact tattaatagt aaaataaagt
     8641 gaatcaatta tattataatt gctaaagaga gtgaggtgta gctatttgaa aaaaaataga
     8701 ttaatagcaa agaatatatc aatagcattt atagtactat tcttttttag tgtatttaca
     8761 tttttttatg taggtaatat taatagagtg ttagaatatg aaacaaatga cataataaca
     8821 gttacaattg ctggatggat tatactttca tttttatttt taggtattat aatttacata
     8881 ttatattcaa aagcaaatag tcaaaaaact atagaaaaag tagcatatac ggattttgta
     8941 acaggttatt ccaactggag aaagtttgaa ttagatgtta caaatttatt aaagaaaaca
     9001 tcacaaaata ataaatatgc aatggtcata ttcgatattg ataaatttaa agcaattaat
     9061 gatatatatg gtcataaaaa gggaaattta atattaaaag atattgcaga tactttaaat
     9121 gaattgacta atatcaatga aacttttgct cgtgtaagtg ctgatagttt taatatatta
     9181 ttaacatata acaaaaaaga ggatataata aatattatca aaaagattat ggcaaataat
     9241 gaacttgtaa acttatcttt tggaatttat gaaatcaaag ataaggattt atctgtaagt
     9301 gtatatagcg atagagcttc acttgcaaaa tcatcaataa aaaataatag tgatgtaaat
     9361 tttgcatttt ttaatgataa attaagagag aaattattac ttgaagataa gatagaaaaa
     9421 gaaatggaat atgctcttga aagtgggcaa ttcgtaatgt atttacaacc aaaatacaat
     9481 attaaattag ataaattttg tggaagtgaa gccctagtca gatggcaata tacagaaaaa
     9541 gaggtcatat atccaggtga ttttattcct atatttgaaa aaaatggatt tattagaaag
     9601 attgatatgt acatattaga acaagcttgt aaagaaatcc ggtctttatt tgataaagga
     9661 atttcacctt tacctatatc tgtaaacttc tcaagagttg atttttttaa aaaagatttt
     9721 attgaaaaca tagtaaatat atgtgataga tataaaatac catatagttt aatagaaatt
     9781 gaaattactg aatctagtat gtttggagat actgatactc tatttaatgt tagtagaaac
     9841 ctacaggata ttggctttat agttgcaatg gatgattttg gctcgggata ttcttctgta
     9901 aatatgctta aaaatatacc attaaatgta ataaaattag atagaggatt ttttgttgat
     9961 gataaagatg ttgataagag tcaaattgtt ataaaaagca tagtttctct cattaagcaa
    10021 ttaggaataa gggttgttgc tgaaggaatt gaaacaagaa gtcaaataga aatgttaaaa
    10081 aaagcaaatt gtgatattgt tcaaggatat tatttttcaa aaccattacc aattaaagaa
    10141 tttgaaaaat tagtctataa aatatagtca gcgagtctta aatgggaggt ggtgattaag
    10201 tgataagagc aaaatcgtta aaaaatgtac tgagtattgt tttaatattt ttatttacaa
    10261 taatttcttt ttctgatttt gcatttaaaa ctaatgtcca ggaagaagaa aatagtaaaa
    10321 ttaatttaag ggaaatatcc ctacaaagtt ccaatgctat aaaaataaaa ttagatagtt
    10381 ctctatcctt tattaaaagt gtggcaaata tggtttctta ttttaatact atagataact
    10441 caaaaatagt tccattatta gctaatatat caaaagaatc tgactttcaa agaatctcta
    10501 ttgtatctcc tgatggaata agtcatacca gtgatggcaa aattatcaat gtgtcaaagt
    10561 atcagtattt taagaaagct attaaaggag aaagtaatat atctgaagtt gtgaaatctc
    10621 caattgatgg aaaagatact tttgttatag ctactcctgt atttaataag aataacaaaa
    10681 taattggtgt tttatacggt tcttactaca atgataaatt agctgaattt atagatataa
    10741 caagttttaa tagtgaaggg tacatagata tatttgaatc tagtggtcaa tttgttttaa
    10801 aatcttcaca tacaaacaaa ctgattaagt caagtagtaa catatatgat atatttaaaa
    10861 aagcttcttt tgatgatggt tatagctata aaaaaatata tgaagataca aaaaataaca
    10921 agagtggctt tacctcatat gaatacaaag gagaaaatcg ttatgctact tatacaccca
    10981 ttggtgtaaa tgactggtat gtaatttctg tgataccaca atatactgta agaagaaaca
    11041 ttgataaaat aaatcaatta gcattttctc ttattttcaa ggtattctta ttgtttatta
    11101 tgcttataat atatattatt tataaaaaca ataaatctca gaaagatata ctaaaagcta
    11161 acaaagaaat ctcatctatt acaaatagca ttcctggagg agtacaaaag tgtaccctgg
    11221 gtgaaacatg caatattaat tttttaagtg atggatttat agagttgttt ggttatacaa
    11281 gagaagaaat aaaaacattg tttaataatg atttttatag tttaatacat aaagatgatg
    11341 ttgaaagaat aaaaaaagaa atttttaaac aactttacaa aaataaaact attgaattag
    11401 aatataggat tataacaaaa aatggtaata cagtatgggt tttagataaa ggggagctta
    11461 taacagaacc tgatggaaaa gaatattttt gttgtgtaac tgtagatatt actagaacaa
    11521 aacaattaga acaagagatg gaacaaaaaa atctagaact tgaaatgata tcagaaaatg
    11581 ttttaggtgg tataattata acagaattta atgatgattt tactataaaa tacgctaact
    11641 ctggttatct ttcaatgatt ggatatacac gtgaacaatt agaaaatgaa cttaaataca
    11701 aagcttgtaa tcttgttttt tatgaggata aaaagaaggc tcttgaaagt atacaacatc
    11761 aattaaacac aaaaggaata gctgtatatc aacatagaat aagaagaaga aatggtaata
    11821 ttatttgggt ttctataaaa ggaaggctta ttgttgatga tgatggtatt gaaaaaggta
    11881 tttggattct aaatgatata acagatttaa aaaatgcaga acaaaaactt aaaataaatg
    11941 aagaaagatt tagaattgca ttaaagcaaa catcaaacac tatatttgag tatgatataa
    12001 aaacaaagtc acttttaaat actagtaaag agcttgataa atataatatt tataaaatta
    12061 ctaaaaacgt tccatattct ctagtagaat caggagttat tcataaagat tttagtgatg
    12121 attttataaa catgtatgaa aatgtaataa atggagcaaa aaaagcaagt tgtgtagtta
    12181 agatgaaact tccagatgaa aattatatat ggaataaagt taccttgact actatttttg
    12241 atgaagatgg taatcctgta agagcaattg gtatacagga agatataagc aaacaaaagg
    12301 aagcagagat aagatacaat caagaaggta agtatcattc agctatattg tcggaagcaa
    12361 tgataactta tgaaataaat gtatcaaagg atacttttat caaaggaaat gaaagttggc
    12421 aaaaaccatt tggaattaag cctgacaata actattctca aatggtaaaa actatagcaa
    12481 atagtattgt acataaagac aataaagaaa catttttaaa tacattttta tgtgaaaact
    12541 tattggatgc atataaaaat aatgtgactg aaataaaatt agaatatcga agaatgaatc
    12601 aagaaaataa aatgatttgg gtcatttgta caatgcactt aataaaagat tctattacga
    12661 acgatataaa agcacttgct tatgttaaag atattgacaa gcaaaaaaat gaagaaatta
    12721 gattaaaatt aaaagctgaa agagatactc ttacaggatt atataataaa ggtactacag
    12781 aaaaattgat taatgatttc ttgaactctg aggaagcaag tttaggaatg catgcatttt
    12841 taattgttga tatagataat tttaaatctg tgaatgataa tttaggacat gtatttggag
    12901 ataaagtatt atttgaagtt tctaaaaacc taaaacctat atttagaaaa gatgatatag
    12961 taggtagaat tggcggagat gaatttatag tattcttaaa aaatattcag tcttttgaga
    13021 atatcaaaaa gaaagcagaa aaaatatgcg atatatttag aaattcttat acagaaaata
    13081 aaaagacata taaaatatct ggaacagttg gaatttcatt tgtaccagaa catggaactg
    13141 tatttgaaga actttataaa aaagcagaca tagcacttta ttatgcaaag agcaaaggaa
    13201 aagataagta tgcactttat aatgattgta tctaccaaaa tgaacttgaa aataatataa
    13261 gtgataataa tataaaatta gcagataaat gtgagaaatc caaaccagta cttaaagaaa
    13321 ttcttgattc tattgataat tatatctata tagtgaatcc aaatacttat gaacttcttt
    13381 ttatcaataa taaggtgtct cgtttggatt tagagataaa attaggagaa acctgttata
    13441 aatcactatt aaaatctgat agaccatgta ataattgccc tcttaacgga ttagaagaaa
    13501 ataagcattc aaaattcaaa atagagacaa atacatttgg taaaaatatc agtatagcaa
    13561 gttggattaa ttgcatagat ggagaataca actgtcttat agagtgtata gattcttctt
    13621 tatacaaaaa taaataaaaa aggactatga agatatgtta aatagtataa ctttgatact
    13681 tggtataata tttacaattt actattttta tctcaaaata gtttttggag gaatttcatt
    13741 tagtgagatt ttcttagttg taggaattgt actaattatc tatcaaatat ttaagaaaaa
    13801 aattaaagaa aagaaggttt tttatagggt gttaaaaata ataatcagca tttttttaat
    13861 agtatttgta gttactgaga gtctaattat tttttatccc caaaatagtt tagaatcaaa
    13921 aagtgattat ctcttaattt taggagcttc tgtaaaaaag actacaccaa gtacaaccct
    13981 gaagggtagg ttagacactg cattgaaata cctaaaaatt aatgataatt gttatgtggt
    14041 agtatctggt ggtaaaggaa gtggagaaaa aataactgag gcaaaagcta tgaaagatta
    14101 tcttataaaa aatggtatag ataaaaatag gataatagaa gaggataaat ccacgaatac
    14161 ttatgagaat tttaaatatt caaaattgaa gatagaagaa catagtcaag gaaaactaag
    14221 cgatttaaaa gtaaaaatag taacaacaga ttttcatgtt ctcagaagta aaatattagc
    14281 atatagaaat ggatataaaa atacgtcttt ttatgctagt aaatcaaaac tttcatttgt
    14341 accaacttac tatacaagag aattttttgc attatggaaa acaatcattt ttgatagata
    14401 ataaataaaa agactagata taaactcata tctagtcttt tctattccct taattctaat
    14461 ccattttcca actttacttg ttgtagaaat ttcaacattg ggatttacaa gtaattgctt
    14521 ataagaagtt ttatatttta cgattccaaa atataatttt ccttcataat ccattataaa
    14581 tccgaatggt cttactcttg gtttattatc ttcaactgta gcaaaataga atgttgagtt
    14641 ttctttaaaa aaattcatta ttttttcatg attaaatcct ctttgtatct tatactaata
    14701 taatgaatat aaataaaata ttcataatac taatataaat cgatataaaa acaattacaa
    14761 tattatattg attattcaaa attatatata aaattcagaa aaaaatttta gtttacataa
    14821 taaaaataaa gtcaactaaa attattgatt ttagattaaa gtaccggatt aagcatttaa
    14881 gtagatttta agcttgattt gattagaaag gtacaaacta tc
//
LOCUS       DAELNY010000086        14898 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.086, whole genome shotgun sequence.
ACCESSION   DAELNY010000086 DAELNY010000000
VERSION     DAELNY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.086"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..1006
                     /locus_tag="KPE55_002207"
     CDS             164..1006
                     /locus_tag="KPE55_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0493270.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            1021..1485
                     /locus_tag="KPE55_002208"
     CDS             1021..1485
                     /locus_tag="KPE55_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493271.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1472..2644
                     /locus_tag="KPE55_002209"
     CDS             1472..2644
                     /locus_tag="KPE55_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0493272.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2688..3623
                     /locus_tag="KPE55_002210"
     CDS             2688..3623
                     /locus_tag="KPE55_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG0493273.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3994..4719)
                     /locus_tag="KPE55_002211"
     CDS             complement(3994..4719)
                     /locus_tag="KPE55_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0493274.1"
                     /translation="MLESKIRTPEEIKRYMLDLKKWLAQERSKPVEEMSDFFSKRIDI
                     YDNVHLANWTEEYAHIADYFDTPLSSLLDIGCGTGLELASIYRKFPNVQITGIDLSKE
                     MMSKLKEKYSDKNIDLILADYFEYPFEQEKYDAALSFETLHHFKYQKKQKIYKKLFQT
                     IKPGGYYVECDYIACCLEEETICLEQYEFKCKENHVPDDVFVHIDIPLTIEHQIHLMK
                     NAGFRAVYVLHENNGTMIIKAEK"
     gene            complement(4737..5303)
                     /locus_tag="KPE55_002212"
     CDS             complement(4737..5303)
                     /locus_tag="KPE55_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493275.1"
                     /translation="MPPKPIITKQDIINAAIQLVRENGISSVNARNLAKTLNCSTKPL
                     FRIYTNMDEIKEDIKIELDNYYNSFMESKINDENRLLSQGLAYIEFAQNEKMIFNTLF
                     MNMTMKGSSLQDIIHAKWNRITIENAKKVTGLSIEKSEMLFINFWLYSHGVATQIISN
                     GIDIPLDMVQQLLENAFERFSLDITKTE"
     gene            complement(6214..6612)
                     /locus_tag="KPE55_002213"
     CDS             complement(6214..6612)
                     /locus_tag="KPE55_002213"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0493276.1"
                     /translation="MKWKELYNKARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(6911..9604)
                     /locus_tag="KPE55_002214"
     CDS             complement(6911..9604)
                     /locus_tag="KPE55_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0493277.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     SATPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGSGTPAT"
     gene            9818..10516
                     /gene="yunB"
                     /locus_tag="KPE55_002215"
     CDS             9818..10516
                     /gene="yunB"
                     /locus_tag="KPE55_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG0493278.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(10728..11735)
                     /locus_tag="KPE55_002216"
     CDS             complement(10728..11735)
                     /locus_tag="KPE55_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0493279.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            11963..12847
                     /locus_tag="KPE55_002217"
     CDS             11963..12847
                     /locus_tag="KPE55_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0493280.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            13049..14599
                     /locus_tag="KPE55_002218"
     CDS             13049..14599
                     /locus_tag="KPE55_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0493281.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            14628..>14898
                     /locus_tag="KPE55_002219"
     CDS             14628..>14898
                     /locus_tag="KPE55_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HBG0493282.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVR"
BASE COUNT         5244 a         2128 c         2491 g         5035 t
ORIGIN      
        1 gtattttata tcatgaatct ataatatttt tttgattcat aactaaaagt atataaaaat
       61 gatgaataaa tatatattat acattatcaa tgataggtta tataataaaa tcactaacaa
      121 ttatattata agaaaattat aaaaattaca aggaggtata gaaatgaaag aaaaagcagg
      181 gattagaaat gccataatag cagcctttat aatcacaata ttggcagaat taattggacc
      241 tatttcattt aaagttatgg gaattaatgt aactttactt agtatactat gggctatatt
      301 cataggaatg gctgtttctc ctcatttatt agggagagtt ataccagcct taaaaaagtt
      361 tataggggat aatgaaataa atgtatctcc atatttatta tctttgacac tatatccatt
      421 agggataatg tttggaatta atgcagggcc taaagttggt atattacttc aagcaggacc
      481 agcactttta ttacaagaat ttggtcatat gggtacaatg ttgatagcat tacctgttgg
      541 agtagcatta ggtcttggaa gaagtgcatt aggtggtact ttttcattat gtcgtgacac
      601 agcactagga attattggag ataagtatgg attaaactca ccagaaggta taggaacact
      661 aggaacttac ataagtggta gtatatttgg aacattatta ttttcatttt tagcaccagt
      721 gggtgtactt ttaggatttc atccatatgc tttagcaatg gcatcaggaa tgggtagtgg
      781 ttcaatgatg ggagctgcaa cagcatcatt aatgaatacg gttccacaaa tgtcagagca
      841 gattttagca tattcagcta ctagtggatt gattacagct gttactggaa tatatataga
      901 attattctta tctttaccag tagccaacta ctattatagc aaagtagctc cagttattga
      961 aagatttaga ggaagaaaaa caagaagaac aagtaataaa ttgtaaagga ggatatatat
     1021 atggaaaaaa cttttaagac tattatggag cagataaaaa tagtattgtt tatatatatt
     1081 attatttgct ttggacaaat gatagcactt aagactcagg ctcaagattt gttgattgga
     1141 agtgtatctg gatttttgtt ggtgataatt gctattataa taaagaattt tgttaaaaaa
     1201 cctaatttac caggttttgc atgggctaca ttagttgcat tcgttttatc gcttccaata
     1261 tctccagtgg gggatatcat agcaacaaat gtagggaaaa tcaattttat gtctacagta
     1321 acaccattat tagcttttgc aggaatctct gtaggtaacc aattagatat attaaagact
     1381 ttgagttgga agttagtagt tatatcattt atagttatga catctactta ctttggttct
     1441 gcttttatat ctcaaacagt tttaagatta aatggaatgc tttaaggagg tagtaaatat
     1501 gaatgaatta aaagcaaaaa ttaaagagga aataaagtta ttatctgaag agaaaaagac
     1561 atcatttgaa aaagtttctg actatttatt ttcaaatcca gaattagcat ttgaggagta
     1621 taagtcacaa aaagcattat gtgatttgtt agaagaaaat gaatttaatg taactaaagg
     1681 tgtaggagga ttagaaacat cttttgaagc tgtgtattca aatggaacta atgggaaaac
     1741 agttgctttt ttagctgaat atgatgcact tccaggaatg ggacatgcat gtgggcacaa
     1801 tatcataggg acaagttctg ttggtgcagg tatcatatta aaagaaatca tgaaaaaaca
     1861 taatatagaa gggactgtaa aggtttttgg aacacctgca gaagaaagag taggtggaaa
     1921 aataactatg attaaagaag gtgtatttaa taatgtagat gctgcattaa ttttacatcc
     1981 aagtgatgct tcaatgcctg atgatatatc atttgctcaa gttaatttaa aatttgattt
     2041 tactggtaag gcatcacatg ctgctgcttt tccttgggaa gggaaaagtg ccttaagtgg
     2101 agtaatagca ctatttaaca gtgtaaatag catgagatta catcttaaag attatgcaag
     2161 agtacatgga attataactg atggaggtag catacataat ataattcctg aaaaatctac
     2221 agctatattt aatgtaagag ccttaagtat agaatattta aatgagattt gtgaaatgct
     2281 taaaaattgt gctaaaggag ctgcaatttc tactggaact aatgtagaaa tagttcaatt
     2341 agatgaaata tacaaagaga ttaagaatga ttctgaatta gtgaatatag ttagacaaaa
     2401 ttttgaagtt ttgggtgaag actatgttga aagagattta agtcaaggta taggttcaac
     2461 tgatacagga aatttaactc atgagatacc tgcaatacaa gcatatataa aattaaaaga
     2521 aaatactgca actcatacag atgaatttgc tgttgcagct ggtggtgaag aaggcagagt
     2581 agcacttata aaagcaatta aggtgttagc aatgtgtgga gtagatattt tgtatagtaa
     2641 ataatatata attttaaaat atattaagaa aggaaatcgg attagacatg atattaaaac
     2701 aagtaataga ggtttatgac attttagata aagcaaatgc taatggggaa gaagtaaaaa
     2761 cttacttaca aggttatggt gaagtagatg taactgtaaa agaattgtct tcaagtaaag
     2821 gctcaacaga tttagtaaag cttacaattc ctgggaaaaa tggaaaatta aaaggtggag
     2881 atgctcctac tcttggcatt ttaggtagac taggagggat tggagcaaga ccagaagtta
     2941 taggttttgt ttcagatggt gatggagcat tggtagcaat tgcagtggct gctaaacttt
     3001 tggatatgca acgaaaaggg gatatattag atggtgatgt tgtaatttca acccatattt
     3061 gcccagatgc tccaactaaa gaacactatc ctactccttt tatggattct ccaattgata
     3121 tgatgactat gaatgaaaat gaagttgata gtagttgtga tgcaatactt tctatagata
     3181 ctactaaagg aaatagagta atcaatacta gaggatttgc aatatcgcca actgttaaag
     3241 aaggctacat attaaaaact agtgataacc ttttagatat tatgcagact gtaacaggga
     3301 aatctccatt tgtattccct ttgtctattc aagatataac accatatgga aataatctgt
     3361 atcacttaaa tagtatttta caaccagcag tagctacaga tgcaccaata gtaggagtag
     3421 caattacaac agagatgcct gttgctggat gtgctacagg tgcaactaat tttagtgatt
     3481 tagattcagc tgggagattt gcaattgaag ttgccaagct atttggtaga aataagtgtg
     3541 atttctatga taaagaagag tgggaaatgc ttattaagag atatggcaaa ttaaatagat
     3601 ttcaaacatt tggtatacag taattagaaa aatattaagt ttatataatt tgtgatataa
     3661 atatctatac tatgtaaaca actagaaaca aaagatatag tttaagactt aaaaaataaa
     3721 atatttattt gattacataa ataaggagtg ttctatttag gtaagcgcca ataagtagta
     3781 taacattaaa gctaacctaa ttgttgatat acaagattga aatgaaaaca gtgcgtatta
     3841 taagaaaagg ttacgcactg tttttttcgt cctgttatat agcagaacac tagtttttaa
     3901 gggtatgagt ataaaacttt tttaaatttc atatctacga attttgtaga ttgtccataa
     3961 gcgatatgca ataattagga aaatattttg agtttatttt tcagctttaa taatcattgt
     4021 gccgttgttt tcatgaagta catacacagc tctaaagcct gcatttttca ttaagtgtat
     4081 ttgatgttca atagtcaaag gaatatcaat atggacaaaa acatcatcag gcacatgatt
     4141 ttctttacat ttaaattcat attgttcaag acaaattgtt tcttcctcta aacaacaggc
     4201 aatataatcg cactcaacat aatatcctcc aggtttaatt gtttgaaaaa gctttttata
     4261 tatcttctgc tttttctgat atttgaaatg atgaagcgtt tcaaatgaga gggcagcatc
     4321 atatttctct tgctcaaaag gatattcaaa ataatctgcc aagattaaat caatattttt
     4381 atctgaatat ttttctttaa gcttactcat catttctttt gataaatcaa tgcctgtaat
     4441 ttgtacatta ggaaattttc gataaatcga tgcaagttct agaccagtac cacaaccaat
     4501 atcaagtaaa gaagatagtg gagtatcaaa ataatctgct atatgtgcat attcttctgt
     4561 ccaattagca agatgaacat tatcataaat atcaattcgc tttgaaaaga aatccgacat
     4621 ttcttcaacc ggtttacttc tttcttgcgc taaccatttt ttgaggtcaa gcatatatct
     4681 tttgatttct tcaggtgttc ttattttact ttccaacata taaaacagtc ctttctttat
     4741 tcggtctttg ttatatccaa tgaaaatcgc tcaaaagcat tttcaagtaa ctgttgtacc
     4801 atatctaatg gaatgtctat gccattggaa ataatttgtg tagctactcc atgagaataa
     4861 agccaaaagt taataaatag catttctgat ttttctatag ataagcctgt aactttttta
     4921 gcattttcta tagtaattct gttccatttt gcatgaataa tatcttgtaa ggatgaaccc
     4981 ttcattgtca tattcataaa taaggtattg aaaatcattt tttcattttg agcaaattca
     5041 atgtatgcaa gtccttgact taacagacga ttttcatcat ttattttact ttccataaaa
     5101 gaattatagt aattgtctag ttctatttta atatcttctt ttatctcatc catatttgtg
     5161 taaatacgaa aaaggggttt tgtagagcaa ttaagggttt ttgctaagtt acgagcattt
     5221 acgctactta ttccattttc tcttaccaac tgaattgcag cgttaataat atcttgtttt
     5281 gtaataatcg gttttggtgg catcttttca tcctcctttc ggtaacttaa gttgcgcaac
     5341 tggtgttgtt attataacaa aggcatataa ctgtgtcaat agacttcaaa gaaaagctac
     5401 agacatcgag tgtaatatga tttatttcaa taaataccat aatatgtgta cttatatctt
     5461 aaaaaggaga taaacagtaa aatgtagttt agttaaattt gaaacttcgt caaagcttct
     5521 ttgtacgata agacaacgta caaatcattg tatgggtaac agagaaagaa ctgcgttgat
     5581 tgaggatgaa atctcacatg atacgatgta attataaaaa aaggagcgtt tcaaagtgat
     5641 ttttttaatc atcttgagac aactccttat attttttatt taaattctat tttttattgc
     5701 ctgtatagct attttctcca tcataaggtg gcatgacctt gaacagcgtg gttttgtaaa
     5761 gttgggcacc acaaaacaaa aaatcaacaa gcccttgtgt ggtctgctga cgtgtttact
     5821 atactatact ttatccaaaa ccgcaagcac tatgggaggc tataaggcgg cggccattca
     5881 ggggcgatcc cctcgaaacc gggatagggt tcccatgtgg gacataataa agagcgcccc
     5941 ggaggtatca actagcatct ggggcgctct ttattatgcc ccgtgggaac catgcccacg
     6001 aagcagggcg tacaacggac agataggtcc taccctcctg acaaactgga atctccgaaa
     6061 acaatcataa caatcatggc aagattatat acactcctgt aaaatatttt tgtgtgtacc
     6121 atgaagtact gcttgccact attgagaacg gataaaaggg aagtacgtat tataccaata
     6181 aggtgcatga aacaaaatac tcatttgata aagttaaata aacctgtcgg aaccccacca
     6241 atccggcaaa agctcactca atttaacagt tttttctgtt tcgacattaa ctaaaatctc
     6301 aatacaagca ctatccttat caagttgcat taaatattca cgacaagcac cacatggtga
     6361 gccaacactc ccattgctca tcacacaaac taatttagta attttacttt cgccatttgt
     6421 tatcatattg gcgatagcat ttctttctgc acacattccc aatgtacagg ctgtatcaat
     6481 acacacacca gtataaacat ttccgctgtc ggtaaggatt gctgcggcaa ccccaccagc
     6541 atcgataaaa ggtgaaattg tgcgtggatt taattttccc ctagccttat tataaagttc
     6601 cttccatttc atcaggttga tcctcctgtt gtatccatat aaataatctt ttatacgcct
     6661 tttccgccta aggcacacaa cacattactc gcttttttca ttctaatatt cgtgcgtgaa
     6721 aatgtcaaac ggtttacttc gtttcattac ttcatactgc tgttcataac cctgtttgtt
     6781 cacttgggca ccattagagc accaatttac ccgtttcagg gttgtagcga acacagcaga
     6841 aatgctcatt tcatgggctt ttctggctag aaaaaaaatt ttatcgctgc cagatattta
     6901 ctatttttta ttaagttgct ggagttccac taccatcagg aggagtagta gagccaccac
     6961 cattatttcc accattatca ggaggagtag tagagccacc accattgttt ccgccattat
     7021 caggaggagt agcagagcca ccaccattat ttccgccatt atcaggagga gtagtagagc
     7081 caccaccatt gtttccgcca ttatcaggag gtgtagaacc gccaccatta tcacctgtgt
     7141 tattattgtt attattattt tcttctggtt ggttattgtc tggttttttt gtatcttcag
     7201 atgatggttg agaacttact ttagtaggag cagtaccttc aataaatgct gctttagagc
     7261 cagaagattg ctttcctcca tcctctaaat taatttttgt aaaatataca tttttaggaa
     7321 cttcaaattc agtaaccgtt aaattagcat gtattttttc cataattttt tcccatagtt
     7381 ttgcagtact agtacttcca tgtggtactt ctctacgttt tatagtgtta ccagaatcat
     7441 cttttctacc agcatcatct ccaatataag ttgcaccaac ataataagga gtgtaaccga
     7501 caaaccaagc atcatatgca ctatttgtag tacctgtttt acctgctaca ggcattccct
     7561 ttggaagtgc agcaacacca ccagtacctt cagtaattac actttctaac atatctgtaa
     7621 cgacataagc tacttcaggt tctacaactt tatgttcctc aggagcattt ttaactaata
     7681 attgaccatc aaaggtagtt atagtagtaa aaatagaagg ttctacataa ataccgtgat
     7741 ttgccaatgt accataagca gcagccattt gaagtggagt aataccattc gccataccac
     7801 ctagtgtcaa tgatgggaat tttctatcac ttgcttcaga tgaacctgag tgattattct
     7861 ttaaagttgt tataccaaag ttctttaagt aatccatcat tatatctaca cattcatcat
     7921 aagagtctcc aagcatttca gcagttttga cagctatcgt gtttgaagat ttagcaagtg
     7981 ctttacgtaa actcatagaa ccatgaccag cagtagtagt tcttggattc catttattat
     8041 tttcttcaaa cttatatcct cctcgagaat cacttagagc agttgcttgt gttattttta
     8101 gagtatcaat agctggcgta tatattgcta aaggttttat agaagaacct ggctgatgag
     8161 ggttagtagc acgatttaaa gtttttctac cacttatatt tcttcctcca gctagtgctc
     8221 ttatttcacc atttttataa tctaagatta ccattgcagc ttgcggttgg ctaactccat
     8281 caggaccgac catatgtcct gggaaattgc tatttctatc aaattcttct tctaaagcat
     8341 cttgcatttt agggtctata gttgtgttta caattagacc accattgtag aataagtttt
     8401 gagcttcatc atttgtatgt ccttgttcca ttaaagcatt tataacttca ctttcgataa
     8461 ggtcttctac agatgaagaa actttatctg atgattgagg caattttatt tctattttag
     8521 ctttgattgc ttcatcatat tcactttgtt ttatatattt caattctaac attttcttta
     8581 aaacaatttc ttgtcttttt ttagcttcag gattacttac agcctttcta acaactaatg
     8641 tacctgcttt taattgtcta taagtatcat tagatataag ctcccaagat tttatttttt
     8701 caatcatatc aaagtccact tgagtagggt catctaaatc atcatcagtt gtgttaacaa
     8761 aaaataataa tttattctct aaatcctcct tagtttcatt tccctctaac ttagaagttt
     8821 tataggctga aaatcttgat ggatttttag ttgaaccagc aagaagtgct gactctgcta
     8881 gagtaaggtc agctgcagac ttatcaaaat aaccacgtgc accagcctca gcacctgcta
     8941 gacctctacc aacgaagaag ttatttaaat aagtttctag tattttatct ttacttactg
     9001 ttttactcat ctcatgtgca tagtaaatat cctttatttt acgaggtata gtttgctgtt
     9061 cagttgttaa cagcattttg gatacctgca taggaatagt actaccacct tgcttagtac
     9121 ctgtaagggt ctttaatact gacctaaata aacctttaat atcgacaccc ttatgctcat
     9181 aaaaacgctc atcttctatt gcaattacag catgttgaag ataaggggaa atcttatcta
     9241 atggaacggg tgtcttttta tttacacttg gagctgtaga taatgttttt ccattagcgt
     9301 atttaatagt agttgtttgg ttcgtctgtt tatctaaaac agcttctgta actggagtta
     9361 catctcttag tgacgcaaat actagaccag cagtacctgc tgtaccaaca actagtaaaa
     9421 ctaaaaatac tatcccaaat actcttagtt ttttaaattt atcgcttttt ttagattttt
     9481 tcttgttttt tgttgaacga gtcttgttag ctgaagttct attaattggt ttatttgtat
     9541 taccagatga gctaactttt tttctcctga ttttatttcc atttttatca ttatcgttat
     9601 tcataagatt ttaacctcct tacttaatgc aatattataa cacaaatagg agtagttttg
     9661 taattaatag aaaaaatgaa tttgctataa tattctgtac attgtatatg tataaacgta
     9721 atatattaaa aagtctatat ttttatttta tttatttttg acaaatatta taaaaattat
     9781 gccaaagcta catataaata aataggaggg gataattttg aatagaatag tagatagaaa
     9841 aacaaaaaat gaatttagaa aaatcatagc tattttttta gtaattcttt tcttgtctgt
     9901 atttataggc agttttatat atatagataa aactttaaga ccgacaataa cagttttagc
     9961 agagactaaa gctcttgaac tagcaaatag gtcgataaat aaagctgttg ctgagatggt
    10021 agaaggaaaa ataaattatg aagatttaat ggatatacaa cttgataata atggtaaaat
    10081 aactatgata caagcaaaca ctataatgat gaatgaaatt gcatctgcaa tagctttaga
    10141 aatacaagat gaattaaaaa aggataaaac agcaagttct tacataccta taggaacagc
    10201 attaggaagt cctatattag ctaaatacgg tcctaaactt gaagtatcta ttgaacctat
    10261 aggaacagta tcagtaaact ttaaaacgga atttgagtct tcaggtataa atcaaactag
    10321 acatagaata tatttagaag ctcaaacaca agttaaagta gtaataccac ttataacttc
    10381 aacaaaacaa attaaagctc aaatacctat ttgtgaaact ataatagtag gtgatgttcc
    10441 agaaagttat gtaaatattc cagaaaaaaa tctaggaaat gttttaccta acactggaaa
    10501 gaataccaac aagtaatata aagttgtata tttttaaata aattcaaaat aatagtttat
    10561 aaataaaatt tttggaaata atattaatat aattttaata aaaatttata ttttatacta
    10621 tgtaaaatat ctaatatatt tggtctgtag gttaaataaa agtaccttta gaccaaatat
    10681 attattaata acttaataac atcttaaaac ttatttattt atattattta caacatccat
    10741 ttaataaata caccataacc tacagctggg tcgttttcaa ttgcatgtga cactgctccg
    10801 attattttat tatcttgaac tattggagta ccactcatac cttgtacaat accaccagtt
    10861 tgagctaaaa gctttttatc tgtaatttta attttaaaac ttttactttc tggatgtttt
    10921 tggttttcta tatctataat ttcaatatta tattttttac acttattaaa gttatcttgt
    10981 aaaattattt gagcttttcc taatttaact tcatctaaag atgctacttt atattttttt
    11041 aacttggaaa tatcaaagtt attaatttga cctcttattc caaaatcagt attttctgta
    11101 aagctaccaa taatattttc tcgttttgca tttatacatc caacactacc tttataagat
    11161 ttttctatat taaggtgctt agttgtagaa attgaacctg ttttaatagg tatttttctt
    11221 gcattaccca cacttattgg atgacctaca gctccaaact ctgatgtatc tggattgata
    11281 tacgttaaag tagcaaatcc agaaagtaaa ttgttaaaac taattttttc taatatgtgt
    11341 ttggtagttt tcattgttat aagttgtcca cctctattta ataaaataga tacttgtttt
    11401 tcttctggta aagcatttaa ggtattggca aagtcactat atccatcaac ttcagcatta
    11461 tttaccttta ctatagcatc acccaatcta aatggagatg cttctccagg atttctaact
    11521 atgatatttt ttaattctgc gtcaatttga agtacgtttc caattggtat taaatattca
    11581 ggttcatttt tttgtaaatt ttgtgcataa attaaattat ttgagaaaaa atataataaa
    11641 aataaaaatg ttaaaacaat tgtaaatttt gacttagcta agttggtttt tttacttaaa
    11701 gtattatttt taaaatgaaa atttttaagt tgcataaaac acctctttct tattaaatta
    11761 ttttaataat agaattgaag ttttcttgct gcttctatat tatgttgtgc aagaatcatg
    11821 ttttgatgct tggaaaacta gctcaataac accttagttt tcataaaaat aaataaaata
    11881 aatagtgtat atgataaaat aaatacatat tcatataaat aaaaataaac aataactgtc
    11941 tagcataaga aaggagcaaa aaatgaaaaa atatagattg ttagttactt gtataattgc
    12001 catgggaatc ataattatag gaacatcaaa tccagttagc aatttttaca agactacaaa
    12061 tttgattagt tcaggaaata tcacaaatga aaaagcagat aaagatgaaa attcaaacaa
    12121 aaataataaa gataaaaatg aagacaaatc taattctaaa agtaataaag ataagtcatc
    12181 agaggacaaa tcgtcagata ataaaaatga aattcaaaaa aataaaaaac ttttaatttg
    12241 tatagaccca gggcatcaag gaaaaggaga tagcaattta gaaccagtag ctccaggttc
    12301 ttcctctaaa aaagcgagag tatcttcagg aacagagggt attgctacaa aaaaaccaga
    12361 atatgtttta aacttagaag catctcttgt tttaaagagt atattagaat ctaaaggtta
    12421 taatgtaata atgactagag agacacatga tgtaaatata agtaattcag aaagagcaat
    12481 acttgctaat gataaaaaag cagatatggt tgtaagaata catgcagata gtttaaataa
    12541 ttcttcaaaa actggagcat ctatattgat tcctgaaaaa gatggaaaat atacagctcc
    12601 tatatatgaa gaaagtaata aatgtgctga atttatcaaa cagaatatgg aacaatcagg
    12661 tatacaaatc aatggaatcg ttcaaagagg cgatttgact ggattcaact ggtcaaaagt
    12721 accagctgta ttagtagaaa tgggatttat gagtaactat aatgaagacc aaatgatgtc
    12781 aaatccagac taccaaagaa aaatgatgca gtgtatagct gatggattgg atgcgtattt
    12841 taaataagat atgaatagag aaaaagttag gttggtatta ttttcctaat ttttatctct
    12901 attttttatt gtacataaca caaagaagaa gtttatttta ggttaaattg tcgtaaatat
    12961 aactaaaaat attgggaata cttgatttaa gtttaaaaag aatatattat agtatagaat
    13021 gtgtaaaatt tttttgagag gtgttgaaat gctagtagta gaaaatgtga gtcatggatt
    13081 tggtgcgaga acaatattag aaaatgtatc ctttagactt agaaaaggtg aacatattgc
    13141 actagttggt gctaatggag aaggaaaatc gagtttttta aatataataa caaagaagct
    13201 tatgccagat gctggaaata taaaatggtc ttcaagagct acagtaggtt acttagacca
    13261 acatacggta ttaagcaagg gaaaaactat aagagaagtg cttagagagg cttttaagca
    13321 tatgtttgac ttagagcaag aaatgattgc tatgtatgac aaaatgggag aagctagtga
    13381 tgatgaaatg agtaagcttc ttgaagaaac agcagagata caaactatac ttgaaaatag
    13441 tggtttttat atgatagatg caaagataca agaagttgca aatggtcttg gtcttggaga
    13501 aattggtctt gataaagatg taactgactt aagtggaggg caaagaacaa aagtacttct
    13561 tactaaactt ctacttgaaa atccaactat tcttatattg gatgagccta caaactattt
    13621 agacgaagag catataacat ggcttactaa gtaccttcaa gagtatgaga atagttttgt
    13681 cttagtttct catgatattg aatttataaa taatacatgt aatgtaatat atcatatgga
    13741 aaatggagag ttaaatagat ataaaggaaa ttatgatgaa tttgtaagac ttaatgatat
    13801 aaagaaacgt caagaagaac aagcttatga taaacaagtt gaagaaagaa aaagactaga
    13861 ggattttgta gctagaaata aagctagagt agcaactaga ggaatggcta acagtagaca
    13921 aaagcaactt gataagatgg aaatattaga aagaccaaaa gaaaaaataa aacctacttt
    13981 tgcttttaaa gatgctagag cagctagtaa aataattttt gaaacagaaa atttagtttt
    14041 agggtatgat gaagcattga caaaaccact taatttccat ttagaaagag gaaaaaagat
    14101 tgccttaaaa ggaatgaatg gtattggaaa atctacccta ttaaaaactt tacttggaat
    14161 aataaaacca tttgaaggaa atgttaaatt aggtgactat ttggaagttg gatattttga
    14221 acaagagagt tcaagagaaa atagtaatac acctatggat gaagtttgga gtgaatttcc
    14281 agggcttaca aactttgaag ttagacaggc tcttgcaaaa tgtggactta caaatgaaca
    14341 tataacgagt caaatgagag tattaagtgg tggagaagct gctaaggtta gactttgcaa
    14401 agtaatgtta aaaaatataa atttcttagt attagatgag cctacaaatc atttagatgt
    14461 tgaggcaaaa gatgaattga agaaggctat caaagagttt aagggaacag ttcttttagt
    14521 atgtcatgag ccagaatttt attcagaaat tgttgatgat gtatggaata tagaagattt
    14581 tacaactaaa atcgtgtagt gatttaactt attgctgggg ggatttcatg aaaaagtttc
    14641 taaatgtact tttttcaatt tgtattagta ttgttataat agtaggtgtt attaatttta
    14701 ctgttggatt taaacaactt tattattttg acattgatta cttaaatata tcagagttat
    14761 ctggtttatc taaagatgat ataaaattga actacgatta tctaattgat tataacttaa
    14821 ataagaatgt gtctgagttc aaattaccaa cattaaaatc ttctccacaa gggaagatac
    14881 attttgaaga agtcagga
//
LOCUS       DAELNY010000087        14844 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.087, whole genome shotgun sequence.
ACCESSION   DAELNY010000087 DAELNY010000000
VERSION     DAELNY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.087"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..582
                     /locus_tag="KPE55_002220"
     CDS             <1..582
                     /locus_tag="KPE55_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0493283.1"
                     /translation="ISIVLITLIVKLVLMPLSLKQRFSVKKQQELAEKMEYIKEKYKN
                     DTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYALYHVCLDMTKEFTTIIIPWIANL
                     GTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQVKFNKQMAVVTTITSLILTVRTPV
                     AIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            785..1456
                     /locus_tag="KPE55_002221"
     CDS             785..1456
                     /locus_tag="KPE55_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493284.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1453..2583
                     /locus_tag="KPE55_002222"
     CDS             1453..2583
                     /locus_tag="KPE55_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493285.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3014..3859
                     /locus_tag="KPE55_002223"
     CDS             3014..3859
                     /locus_tag="KPE55_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493286.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            3859..4887
                     /locus_tag="KPE55_002224"
     CDS             3859..4887
                     /locus_tag="KPE55_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493287.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            4890..5903
                     /locus_tag="KPE55_002225"
     CDS             4890..5903
                     /locus_tag="KPE55_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493288.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6420..7670)
                     /gene="cme"
                     /locus_tag="KPE55_002226"
     CDS             complement(6420..7670)
                     /gene="cme"
                     /locus_tag="KPE55_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG0493289.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(7756..8502)
                     /locus_tag="KPE55_002227"
     CDS             complement(7756..8502)
                     /locus_tag="KPE55_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0493290.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(8655..9677)
                     /locus_tag="KPE55_002228"
     CDS             complement(8655..9677)
                     /locus_tag="KPE55_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG0493291.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(9677..12097)
                     /locus_tag="KPE55_002229"
     CDS             complement(9677..12097)
                     /locus_tag="KPE55_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0493292.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12183..13103)
                     /locus_tag="KPE55_002230"
     CDS             complement(12183..13103)
                     /locus_tag="KPE55_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0493293.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            13693..14619
                     /locus_tag="KPE55_002231"
     CDS             13693..14619
                     /locus_tag="KPE55_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0493294.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         5335 a         1886 c         2338 g         5285 t
ORIGIN      
        1 atttctattg tacttataac tttaatagtt aaattagtac ttatgccact atcattaaaa
       61 caaagatttt ctgtaaaaaa acagcaagaa ttagctgaaa aaatggaata tataaaagaa
      121 aagtataaga atgatactaa agaattggaa aagcaaatac aagtaagttc tgtagaaagt
      181 atgaagagta tgctgggatg tcttatagtt ttggttcaaa tgccagtagt atatgcttta
      241 tatcatgtat gtttagatat gacaaaggaa tttacaacaa ttataattcc atggattgct
      301 aatttaggta catttgataa tttgtttata ttaccttgta tatatacatt aattatgtta
      361 gcttcaaatt tagtgaatta tataccatat ttaaaaataa attctcaagt aaagtttaac
      421 aaacaaatgg ctgtggtgac taccataaca agtcttatac taactgttag aactccagta
      481 gccataggtc tgtactttat aacaagtgca atttattcat taattgaaga tatttgcttt
      541 agaatatatt ttaatcgaaa aaatagaata gtgacaaaat aatataatta ttattagaaa
      601 tattttagcc tgctaacata gaaaaagtta gtaggctatt tttatctatg atatcaattc
      661 cattaagaat agtctaaaaa atactatcaa aatgctataa aaaatactat aaaaaaatag
      721 ttgaaaaata aataaattaa tataaactaa aataaagcct acaaaaaaga ggtgataaat
      781 agaaatggct aaaatactag ttgtagaaga tgaaaaaaga atgcaaaata ttattgtaga
      841 atatatgcaa aagggaggat atacttgtat aactgctgat gatggagtcg aggcacttac
      901 aatattaaag agcaacaata tagatttaat gatattggat attatgatgc cgtatttaga
      961 tggattttca gtgtgtagag tttcacgtga aatgactaat atacctatta ttatattgac
     1021 agctaaaggt gaagaggagg ataaattaaa gggttatgaa tatggcgcag atgattatat
     1081 aacaaaacca tttagcccaa aagtactttt agcaaaagtt aatgctttgt tacgtagata
     1141 cactactgat attcccaaaa attcattgag tttaggaaag atttttatta tggtagcatc
     1201 aagacaagtg tatgtagaag ataaattgat tgatttaaca tataaagaat ttgaattatt
     1261 gcgtttattt atggaaaacc ctaatcaagt tttttccaga gagaaacttt taaattgtat
     1321 atggggatat gattttgaag gaaacacacg aactgtagat acacatataa aaaccttacg
     1381 caaaaagctt ggtagtgaag gacaccatat agttacatta attcgttcag gatataagtt
     1441 tgaggttaag gaatgaatag tttaagaaag attatagaaa aactgaaaaa tgtagatatt
     1501 tcaaattgga ttaaacaaaa ttttggacta tattcagtta gaaaaaaagt gtttttactt
     1561 tctaaattat caggagcgtt aattatatta ttttatctgg ttgttgaaga attttcgata
     1621 aactctaaat ttgaattttg gatttggttt ataatactgg ttatatttat tatgagtatt
     1681 gatttcttat tgggaaaatt tatttcagaa ccaatcacat ctataaataa atctgcaaaa
     1741 agtatgtccc aacttgattt ttctaatcca tgtactgtaa atactaatga tgaatttgga
     1801 gaactttcta ggagcctgaa tacaatgtct acaaatctac aacaggcatt atcagaccta
     1861 gaaagtgcaa atattcaact tgaaaaggat gtaaataagg aaagaatgct attggaacaa
     1921 agaaaggagt tggtagatac aatttcacat gaaatgaaga ctccacttgg cattatacgt
     1981 gcgtatacag aggggcttat agatgaagtt gatgaagaaa aaaagaaaaa ttatatgaat
     2041 gttataatag aagaaactga tagaatgaac aatatgattg tatcattatt agacttatct
     2101 gcattggaag caggagtttc taaacttaac ccagagcgtt ttgattttat agagtttgtt
     2161 gaaactgtgg caggtcgtct tttaattgat ataccagatg ccaattttta ttttacatat
     2221 gatttgccag agtatgaagt ttttgtagtg gcagataaaa tgagaatgga acaagtagta
     2281 gagaatctag ttattaatgc aaagaagcat gttgtatata atggtaattt agatttatcg
     2341 attacatgtg aaaatggctt gcttttattt gaaatttata atgatggtag gtttatagag
     2401 caagatgaga tttcaaagat ttggtctaag ttttatcgta gtgttcaatc tcagcgtaca
     2461 ggaggttcag gtctagggct tgcaattgtt tcacaaatac ttacaatgca agggttagaa
     2521 tatggagtga ataatcgtga tagaggagta gagttttatt ttatgattcc aattgatgaa
     2581 taatttagta ttaatttata aattataagt atctgatgta aaatattttt atttgtatac
     2641 gtaagtattt aaagagaata aaataaaaaa acttaatgaa aaagcttaat gaaaaagtct
     2701 aataaaaagc ttaatgaaaa gcttaataaa aagtcttgtt ttcctgatga aaacaagatt
     2761 ttttattaag ctttttatta tttcacttcg atttcacaag agagacactc tgatttcacc
     2821 aaatagaaat aaaataggtt aaataaatta aaaaatagaa gcgtaattaa ataaggttac
     2881 aagggggagt acttatgaaa tggtatacat ggttgactat tatattgata atattgcttt
     2941 ctattccgct taaaattaaa tttataaaat ggtgggatag acgacaaaaa aatgataaag
     3001 gaggttatga tagatgataa aagtaagaga gttaaaattt tcttatggaa aagataaaca
     3061 gatattgcat ggacttaatt ttgaagtaaa agaaggagaa atctttggat tccttggacc
     3121 taatggttca ggaaaatcca caacacagaa aatcttaaat ggtgttttaa aaggttacaa
     3181 tggtcacgta tccatttttg gtaaggaagt taaagcatac acagaatctt tatatcaaaa
     3241 gattggtgtt ttatttgaat ttccttattt atataccaat ttaagtgcaa ttgataattt
     3301 agaatatttt tcgtcttttt atcctaaaaa tcaaaggaga gacatatatg aactcttaga
     3361 tttgttagag tttaagaaag agtttataaa taagcctgta tcatcctact caaaaggtat
     3421 gaagcaacgt attagcatgg caagagcatt ggtaagtaat ccaaagttgt tatttttaga
     3481 tgagcctact agtggacttg acccatctgg agctgtacta tttcgtaaga ttattgaaga
     3541 agaacgaaaa aaaggaacaa ctatcttttt gactactcat aatatgctag atgcagattt
     3601 aatgtgtaac aatgtggctt ttattgcaga tggaaagatt atggtaatag ataaacccaa
     3661 aaatttaaaa atgaaaaata gcaataataa agttgaagtg gaatttgtat ataatggaaa
     3721 tcgagatatt gaaattgtag atatacaaga gttagaatct ggaataactt ttaaatatga
     3781 tgaaatctta agtgtacatt caaaagaacc tacactagaa gaagttttta ttaaatacac
     3841 aggaaggatg ttagtgtaat gtattctaaa ttttttacct tagtaaaaaa agatttttgg
     3901 atgatgattt atggtaaatt ttttgtgctg acacttggct ctctcatact ttattcgtgt
     3961 tatattaatt ttggttatgt aaagtttatg gatgaagata gttatcacaa taatgtatat
     4021 ctatataatc cagagaatat aagcatgagt gcatctccat taattaatca agtttcttct
     4081 ataggtgaat taaaagcaaa acttgatgaa gatacaagtg cagtttgtat tgatatgagt
     4141 tccagtaagc cgaaagtaat tttacatgaa agtattaaag gagttgacaa ttatagagct
     4201 gactatgcat tgtcattatt atcatcagaa aaagaccata atagcaaaat atcagacaac
     4261 aatgcaataa ttcaaattaa tgataaggat acatctgaga taaaacaaag aaaagacatg
     4321 acttgtgaga ttttattctt tgaaattgta gctattggat ttttagggat tgcctcttta
     4381 ttatttaaag aaaagcagat gggagtaatg cgtattactg gtattttacc tgtaaaaaga
     4441 agtcttttta tattatcaaa gatatttgct ttttttatat gtgacctatg ttttacagtt
     4501 cttttagtag tatttaatat tggattttta gaaggaataa aaatacttcc acaagtatta
     4561 gttcaagcta gtattttatc aattattatg tctttgattg gaataggatg ctcaatgtta
     4621 ttgagagatt ttaagcaatt ttctcttata tatcttgtgg ttgctgtatt cataactaca
     4681 cctatatttc tatctgcaaa tacatctttt gaaatagatt ggattaaata tcatcccttc
     4741 tatcatgttt atagtcagct taaaaatgcg tttttcatga atttttcaaa agaattagtt
     4801 tattttgtag tgtgttcttt gacaataata gttctatttt tacttgtaaa atatattttt
     4861 aataaagaat tggttaggga gggataatct tgataggatt acgttaccag ttaaagagta
     4921 taaaaaaaga taaaatgtgt ataatttcct tctttttacc agttgttatg gcgattttaa
     4981 taaattttat gggaacaatt gatatatctt cattaggaga gatacgtttt ggtatagtga
     5041 aggatacact tcctgtacaa acagagcagt ggttgaagaa gtatggtatg gtaagtgtat
     5101 atgacacaaa aatgcattta atacgtgaaa ttaaggatat taaaacggac acaattggag
     5161 ttgttagaga tggtaaagga ataaaaactg tgttttatgg aaatgagatt aatgctataa
     5221 aaaatgttgg aaagacattg ccagagatgt ttagactaaa gggtgattta aaagatgttg
     5281 aggttacaat attgcctaaa gatagtattt taaaagagtt tcaaaatgtt tttattgcta
     5341 tgattttaat tactgctatg tttatgggat gcacctttaa tgcaatgaat attatatcgg
     5401 aaaaagaaga tggaatatca tttataaatg aagttttgcc acagagtaga agtcagtata
     5461 ttatacaaaa agtatttttg ggtcttttat gtgggtgttt atcttcaatg ataactgtat
     5521 ttatatgttt ggatatttca atattagata tgataatgat gttgttactc gttgtatttt
     5581 cagcatttat ttcatcactt ataggtttat ttattggaaa gttgtcagaa ggtcttatgg
     5641 taggaattgt ttatattaaa atagtaatga ttttatttat aggtactcct ttgttaatat
     5701 atcttctagg ggttaatacc agtggtatag ggaatttatg ctatataatg ccttcagttg
     5761 ctacatttaa tggtattatg gagattatta atggtaatgt cattattatc aaagatattg
     5821 taattttggc tgtacattgt gtagtatggt ttatgatata tattatttta gataaaaaaa
     5881 gaaagttatc ttttagtaga taactcagta taattataat tgggacaatt taataggtga
     5941 tttaatatac tttgtatagt gtaatttatg tagtgcaatt tgtatacttt aatttatgta
     6001 gtgtaatagt tagttttatt ttataattat tcaatataat ttagtaagaa gttcagtaca
     6061 attagagcta cttagtggaa aacttaatag gtacttaaac tatataaata ctttattttc
     6121 ctccatttgt actataaatt taatttatga tataaatgga ggtattatta tatgatttga
     6181 taaatttcat cattataacc tacaagtaaa ggtattatta tataaagtta ataaagatag
     6241 tatcaaaata ttatttgcta taaagagagg gactcaaaat aaagataaac ctatttttca
     6301 atgttgaaat gataaaatat atttgatttc atataaaaaa ttttcttcat aaattaaaaa
     6361 gtccatttta agccccttca tttttaacca gataaatcga gttaaactat ttaaatatat
     6421 tactcggatt catttgactt agatattcct tgcttgtaaa attgttttga ataggggata
     6481 gccaaagcaa aaaataaaag tataatccct gctatgatta ttaaattatt aatagaaatt
     6541 acatctgcca ttggtccgaa aatcatcata ccaagaggaa taaaaccagt atatataata
     6601 ataggtaagc taagaactct acctagcatt tgtggtgcta catgctcttg caacatagtc
     6661 atagctgatg cttgaatgat tggaattgaa aaattcgtaa aaaacataaa tataaaaaat
     6721 tgccaagctt gtgttacaag acctagaata attgaaaaaa tagcatagga taatattgct
     6781 agaaaaaagg tcttatttct atttttaaaa ccaccaaaaa ctcctataat caatccacct
     6841 aaaaccatac ctaaaaatcc aatcatttca ataatagaca ggtatatata acccttgcca
     6901 aatgttcgtt caataagaag tcctataaga aatcctgatg gaacacaaag aagcatgaat
     6961 gctccataag taaaaagcac ttttctaatg aacttatctg aaaaacagta ctttatccct
     7021 gataccatat ctgcaaaaaa tgagactttt tcagtttgtt ttggtatatc atgtttagga
     7081 attttgatga atattaataa tacaatagca ataattgcag taaatacatc aattaaaaga
     7141 acttgattta ttttattacc aaatccaagt attgcagcag ctacaatagg aactataaat
     7201 tgtactatag actgtagact actatttata ccattaaaac gcattaaatg ttcttctggt
     7261 acaagttgag gaatcattgc gtttacagca ggtgtttgta caccacttcc tagtgaacgt
     7321 ataacagata ttactgatat ggctattaat tcacttgttg tgccatttga tgacatcata
     7381 aatagaatta gaactaatgt agatatagca attgttgaat ctgatacaat tatgagtacc
     7441 tttcgattgt agtagtctgc ccatacacca gcaaaaaaag aaattatcat ttgaggtata
     7501 aaggagcata cagttaaaac tgttacccat acacctgatg atgtctgaag tgtgacatac
     7561 catatcattg ccatttgtac aagtgatgag ccaaataagg aaatatattg acttgctaaa
     7621 aaaagaatga cttttttctt ccatgtagaa ttagtatttt tgttttccat cctattcctc
     7681 ctttgatttg tgtgttgaat ttttcttgct cttaaggata acacacaaat caaagtctgg
     7741 agttagagtg gagggctagg aaaattgttt tttcataatt tgaagatgag atttcatggt
     7801 ttttgcatta tgttcagctt ctttcaatga agttatcagt ttgtcacaca ctgagataat
     7861 ttcgtcatca tcttttggag tgtcaaggac agttttaata ttagtattag gactagaaga
     7921 taaagcattt aacattcgta aaatagctga aagagaatag tttgcacaac gaagtgcgct
     7981 tataatttta agtcgtttta tatcttcatc agtatataca cgataaccat tttgttttct
     8041 ttttactttt aacaaaccat tcatttccca atttcttaaa gtatctattg taacgtttaa
     8101 gtgttctgca gtttcttttc tagttaaaaa gagtttattt gtttctggag tatttccatt
     8161 taaaatctgt gcaacaatag taagtgcttc ctcggcatta gatttttcta tttctaattg
     8221 ttgcaagtat tgatttacaa gactaatagc cttatcaaag tccctattcg ctgatgttct
     8281 tacaatctca agggcatctt ttcttaatcc cctttgtacc acttcaattt tcaaggctgt
     8341 acgagcaaat ctaaattgtt ctatatgaaa atcattaaag atacgataac cattggtatt
     8401 acgcttaggt ttaggtatta agtttagttc ttcatataaa cgtactgtat taggatgtac
     8461 accaattaaa tctgctacct ctgatgtcgt gtaagtgtcc attagtattc aactcctttt
     8521 agatatatag tattagtcat aataacacca aacaaaagtc tggtgttata atggagggtt
     8581 tggctatcta agagtaatta gtttatgaaa gagtaattag tttatgaaag agtaattagt
     8641 ttatgaaaga gtaattaatc tattatagaa gtaagtatat atttttcaat gaactcccat
     8701 ctatcacgag gtttaaacac tattttagat ggtatggcta ttactaagtc atattgtgga
     8761 atacaacaaa tagcatttcc tccagttccc attgctgaat accacaaaac accatctttt
     8821 ttacatagcc accacagata accgtattca ttactattca ttttaactga ttcttcaatc
     8881 cacgatttag atataatacg tttattattc caaataccat catttaaata cagaaaacca
     8941 aatcgcaaca tatctctagc actcaatgta agtccccagc cacctataga ataaccctgt
     9001 ctatcttttg cccaaccctt aagatttttt ccaaagaaat cttcaaaacc aaaatctttc
     9061 atatcataat ctggtataac tttcatacca ataggtttaa ataagtactc attagcaaat
     9121 tcacgagcag attttccagt agtttttgtt ataatagctg aaagtaggtg cgttccagct
     9181 gaagaatatt tgaaagtacc aatttcgcct ttttgaccaa gttgattcaa ggtatatttt
     9241 atccaatctt tttgtttgca aagtctttct aaagactcag tcatattttt aaaagggtat
     9301 ggtgcagtca ttgtaagtag gtgacgtata gttactgctt ttttacatat ttcagaagga
     9361 ctacaagtat attcaggaaa aaaatcaaca accttttggt caacatcttt tatatatcct
     9421 ttatctattg caatacctat taaggctgat ataatacttt ttgtaactga tgcaacatga
     9481 aaagtatcat cagcattata tccattatag tacttttcaa agacaatttt tccttttctt
     9541 gcaataatta taccatttat attgctatat tgtgagatta ttaaaggttc aagttgtgaa
     9601 agcttttctg aactcatacc aacacttgaa gggtctactt tttcaaaaat taagtctaag
     9661 caattgtttt tattcattaa agatacctcc ctgttataga ttttggattt tcttttagag
     9721 tttttggaga accttctgca attatataac ctcctttgtc accaccttct ggaccaagct
     9781 caacaatcca gtcacaaaca tttaatacat ctatatcatg ttcaacaaca attacagaat
     9841 ttccattttc aaccaattca tctaataatt gaatcaactt agaagtatca tatagactta
     9901 gaccagtagt tggctcatcc aatacataaa ggatattacc atttcgtttt ttaccaattt
     9961 cttttgcaag tttgattctt tgggattcac caccacttaa agtcggtgta ggttggccta
    10021 attttatata ccccatacct atttgttcta atacatttaa gtgtgaaaca atatttggaa
    10081 tatctttgaa gaaatgtaca gattcagaaa cactcatatt taaaatatca tgtatatttt
    10141 tatttttata tttaatagat aaagattcat cattaaatct ttttccctga cattcactgc
    10201 atatatggtc tatacttaga ttatttccca accagacctt ttcatatcca ctaccactac
    10261 atttaggaca agcccctttg gagttaaatg agaaatgacc agctgtaaat ccttgttcta
    10321 acgattctgg ctgacctgca aatagtgtac gtatcttatc ccatatacca atataagaag
    10381 caggagttga gttcatattt ttgcctatag gagattgaga tatctctgaa tatccagaaa
    10441 tatgtttagt accaatcagt ctatctgcaa tagtttcaac aactgcataa tcgtcttcct
    10501 cacctatttc actctctgaa gaatttattt gattattgat agtatggtta ttaaattggc
    10561 ttctaagaag aggaagtagg gtgtctgata taagtgaact cttgccacta cctgattttc
    10621 cagatattcc aaccatagca cccaaaggta atgatacagt tacattttta agattatttg
    10681 tatttgcatt ttgaatagtt atacaaggta ttttatcagt atgtgtaatg tttttattgc
    10741 ttgttctatt aggcattgga tattttccag atagatactg tccagtaagt gatttgtcgg
    10801 attgaagtag accttctaaa tctccttgat atactaattg accaccttca acaccagctt
    10861 ttggaccaat atcaataata tgttctgcca tttcaattgt gtttttatca tgctcaacta
    10921 ctatgaccgt attgcctaaa tctttaagtt tctttagtga ttttaagatg tctatttttt
    10981 ctgattcatg caatccagct gttggttcat caagcacata aattaatgag tccatctctg
    11041 aatcgagatg tgaatttaaa aatagtcttt gcatttcccc accactcaaa gttgacattt
    11101 ctcggtataa ggatagatga cctaatcgtg attctattaa atgttgagtt ttggataaaa
    11161 tatcatttac aagatttgta ccaaatgtgt ttaaattacc ttgatttatt aagagtttaa
    11221 ggaaatcatc aatttctaaa atcgtcattt ttccaagttc tccaatatgt tttccgttta
    11281 aaagtactct acgtgcttct tcaccaattt taaaaccatt gcaatctgaa caggcagtca
    11341 tagtataaac tccatttaaa tcttcgcccc ttttgtaacg attgtgaaat acctttgata
    11401 aagaggaact ttttttacca ttttcataat gaccatagag tacttcattt cttatttctt
    11461 caggtagttg taagaattgt atgtctaaat aatcattgaa ttttttagaa aatactctca
    11521 aatatccagg tgtaatcttt gctctatcta agacttgttt tagggtatca tgcttatcaa
    11581 ggactaattt ttcaatatta atctcaaaat atgcaccatg tccagagcac ttcatacaca
    11641 ttccatctga agagttgtat gagaaataac ttggtcttaa tcgttcttcc tcatatccac
    11701 aattattgca aattagatta tcgcttacag gagtatcaca catagtacac attatttgac
    11761 cttccactga atacaatagt gtaagcatgt tgagaatatt ggttcttgtg cctactgttg
    11821 aacgaggatt gctctggcga ataatatttt gttgaacagc tatagtggga gctaaacctt
    11881 gaatattatc aaacttatct tcactatcaa tacctgcaag tatacctaaa gattgcagat
    11941 attgtttacg cccttcctca aatacaatgt caaacataag gctagactta cctgagccac
    12001 tgacacctgt tgcaacaaca agcttatctt ttggaataga aacatctata tttttaagat
    12061 tgtgtatctt tgctccatta atttttattt ctttcattgg ttcacctcat tttatcaatt
    12121 ttatcaaacc gtaaaatagg gatatattaa tccttaatac aagaatagat taggtgaggt
    12181 tattaaaaag gttctatagg taaatatata tcgaccaagt gtctattttg agggtgattg
    12241 tgtgggttgt taagatatac ttcaaaaggg aaggaggcac gtagcttata attaccattt
    12301 gatagccatt cattatacat aaaatcccac gcatcactat attcagattt tgaaatatca
    12361 aaatgtccca caagatattt tccagaaggt attatcatat tgccaagatt gtcattctct
    12421 ttgacaacaa tgttatctgg aatggtcatg caaatactag tccttaattg atgctcctgt
    12481 gtaaattgtg gattatcatg ataaatggct aatatttgtg tgttttcaag tccaagtaga
    12541 ttctgctcac ttgcatatgt gaataaccgt tctaaaagtt ttgggaaagt actggttaga
    12601 ttaccatatg aaccagtata tctagtgtaa atagtattca tattatcaag ttctagaatt
    12661 tcaatttcac ctttaacctc tctagtattc ttacatttag gttttgatat gcttctattg
    12721 tactgagaaa ttttatatgg ttctttgcaa ttcttgctat aattgtttct atattttata
    12781 gggcttattt tatagtaatt tttaaatgct ctcgagaaaa ctgctgaatc agtaaagccg
    12841 aagtagtgtg caatatcagt aacagtcatg tcagttctat gaataagaaa agcagttgca
    12901 ttttctagtt ttagcctatt tacatattgt gccaaagttt ctttttctat tgctttgaag
    12961 attctatgaa aatgaaattt tgaaaaacct gctatattag aaagttcatc aagagaaaga
    13021 ggttcatgta aatgttcttc tatatagtct tgcactttgc aaatacgatt tagatattct
    13081 tttttacttt tagcatttgt catatatagc cctccttatt gtgtaaaatg tgtatcaatt
    13141 aatttatagc aatatagtaa taaaatttta tgtttttgta atttatgata aataagatag
    13201 cgtttaattt tttataattg ataaaagata taagatgcct tatatttgtc tttgcatata
    13261 agtatagtct tcgtaataat tagtatacca tagtgattta tttttaattg tgtatttata
    13321 taaatacaca acttatgata tggacgaatt attatgcaaa taagctgtaa ttaaataaat
    13381 tgcaatataa accagttgaa acataagtta gggaaatatt atcaaagtta ttacttagca
    13441 taagagtata gatggaataa aataagatta aaaaattaca tactaaattt aataaaattt
    13501 ttatattaaa aataatatta agtttattgt ttaaataaaa aagttaaatc tacactatta
    13561 acttattata cttaaaactt taaaatgaaa atatcaaaga atacttttta ataaaaataa
    13621 tcatttaata agttattaaa aaatctaaaa actttacaac aaattatcta tttaaattgg
    13681 aggagtggga atatgagtaa tagtttttat caattaaatt cagcagtatt atattttaat
    13741 gaaaaaagag atgtaaatag aataaggttt aatcactatg taatgagaaa agaaaatata
    13801 caaagattaa aaggaactct gccaagagga caatttatga tgacagtaag gaaagctgca
    13861 tctgaccttg atgtatctat atctacaata agtagacttg taaatgaatt tatagattta
    13921 ggtatattga gattaataag taggggagta aagggaaatt gttgttctgt atatagttat
    13981 gtttgttctg aaagtagtaa tgcagaaatt gtaacaaaga atggtgatat tttcaaaaat
    14041 atagagaaat acattgatgc aactattaac aataaccaaa aaacaatgaa atataaacat
    14101 agaaatgagt tgtttaagaa cagtaatacc aacatataca gccgtataaa tcaagagagt
    14161 atagacaaag attatgattt gataattcat actgggtatg aaacagaaaa tgagacaaag
    14221 aaaaaagaat tattaaaaaa taatttaaat aaaaagaatt taaataaaaa tacctttgaa
    14281 aattcggatg aagatttgac tgaagattct acttacaaaa atccagaaag catacatgaa
    14341 attattataa gaaaattaaa tagtgaatca ggaaaaaatt ttaaagctga tttgagtata
    14401 actaaggcac ttatagataa aagattaaaa gaagggtata gtttagaaga tttttacaag
    14461 gttatagagg caaaggtaag aagatggaga tatacagata tggaaatata tatagaacca
    14521 acaattctgt ttgggtatga atttgagtcg tatttaaatg aggagagttc tttatctgct
    14581 tgtaattatg gtgatatgtg ttatgaagat atgtattgag atatttatta agatattagt
    14641 tgctttttag ttgtttaaaa tatgtttatg tataaatagt gtattaaaat ggagttgtct
    14701 caaaatagag agaagtctct tcttaattgt ttaaatgatg aaaatatatt tgatttaata
    14761 caaaggagag tgctttatga gttagaagat ttattttgag acactcactt tttttaatat
    14821 actggagaaa tataacccat ttta
//
LOCUS       DAELNY010000088        14810 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.088, whole genome shotgun sequence.
ACCESSION   DAELNY010000088 DAELNY010000000
VERSION     DAELNY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.088"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..1307
                     /locus_tag="KPE55_002232"
     CDS             231..1307
                     /locus_tag="KPE55_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0493295.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            1374..3440
                     /gene="bclA3"
                     /locus_tag="KPE55_002233"
     CDS             1374..3440
                     /gene="bclA3"
                     /locus_tag="KPE55_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG0493296.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGITGPT
                     GPTGATGADGATGVTGPTGATGADGVTGPTGATGATGFGVTGPTGPTGATGIGITGAT
                     GLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATGATGPTG
                     ADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLVGPTGAT
                     GATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATGLAGATG
                     ATGPIGATGPTGADGATGATGATGPTGPTGADGLVGPTGATGATGATGATGPTGATGA
                     TGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNA
                     AGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTAVPG
                     ATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYAS
                     AGIAIN"
     gene            3609..4370
                     /locus_tag="KPE55_002234"
     CDS             3609..4370
                     /locus_tag="KPE55_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0493297.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            4679..4957
                     /locus_tag="KPE55_002235"
     CDS             4679..4957
                     /locus_tag="KPE55_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493298.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            5250..5825
                     /locus_tag="KPE55_002236"
     CDS             5250..5825
                     /locus_tag="KPE55_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG0493299.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPNRLSTETLEQFHTNDDNLANQYAMNVELLHRNG
                     AEVGIPEYKIVGQEIDINDLTQVRIVSKYPSQLKVSALLREKLGLSRKAFDEMLDCGT
                     IKSISGVDLRRSKLNYEIVLEVGKQKQKSSN"
     gene            6264..6974
                     /locus_tag="KPE55_002237"
     CDS             6264..6974
                     /locus_tag="KPE55_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493300.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            6968..8284
                     /locus_tag="KPE55_002238"
     CDS             6968..8284
                     /locus_tag="KPE55_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0493301.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDNIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            8296..8436
                     /locus_tag="KPE55_002239"
     CDS             8296..8436
                     /locus_tag="KPE55_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0493302.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            8495..9076
                     /locus_tag="KPE55_002240"
     CDS             8495..9076
                     /locus_tag="KPE55_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0493303.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFVVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            9333..9707
                     /locus_tag="KPE55_002241"
     CDS             9333..9707
                     /locus_tag="KPE55_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493304.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            9790..11499
                     /locus_tag="KPE55_002242"
     CDS             9790..11499
                     /locus_tag="KPE55_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493305.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            11514..12386
                     /locus_tag="KPE55_002243"
     CDS             11514..12386
                     /locus_tag="KPE55_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0493306.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            12387..13175
                     /locus_tag="KPE55_002244"
     CDS             12387..13175
                     /locus_tag="KPE55_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493307.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(13271..14104)
                     /locus_tag="KPE55_002245"
     CDS             complement(13271..14104)
                     /locus_tag="KPE55_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG0493308.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIKMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(14172..14801)
                     /locus_tag="KPE55_002246"
     CDS             complement(14172..14801)
                     /locus_tag="KPE55_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG0493309.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         5819 a         1710 c         2524 g         4757 t
ORIGIN      
        1 tagtttatgg gacactcttt tttgtatgaa attaaatatg gttttatcat ttcaacaatt
       61 attaccaaat ttcaagttta ataaattcta ctataaaaaa tacataaaag ataaggttct
      121 ttagtaactt tctgtcgtca aaagttttta taaatttaat aacaaaacaa gagaaaatac
      181 catatattac ttatgtctgc aatatataga agacaaagga gttgtacatt atgattacaa
      241 taagtttgtg catgattgtt aagaatgagg aagaagttat aggcagatgc ttagaatgtg
      301 taaaggatat agtagacgaa attataattg tagatacagg ttctacagat tccacaaaag
      361 aaatagtctc aaaatataca gacatggtgt acgatttcga atggatagat gatttctctg
      421 ctgcaagaaa tttttcattt tctaaagctt ctaaagacta tactatgtgg cttgatgcag
      481 atgacataat tttagaagct gatagagaaa aattattaaa gattaaagaa agcttggata
      541 cttccattga tattgttatg gcaaaataca atgtatcctt tgatgaaaat ggtaacccaa
      601 ctctctcgta ttatagagaa cgattgttta aaagaagttc taatcataaa tgggttgacc
      661 ctattcatga agtagtacct ctttttggag aggtatttta ttctgacatt gcaatatctc
      721 ataaaaagct tcatagacag gaccctctaa gaaatcttag aatttttgaa aaaatgattt
      781 cagaaggaaa aacattggag ccaagacacc aattctatta ttctagagag ttgtactata
      841 atgctaggta taaagaagca atagaaggtt ttactaagtt tttagactca tcaagaggat
      901 ggattgagga ttgtatcagt gcttgcagag atttagccac atgttattac ttaattaatg
      961 atgaaaaaag tgccttatat tcattgttta gaagtttcga atttgatgag ccaagagcag
     1021 aaatttgttg tgacatagga aagcatatgt ttgatagaca aaaatataaa gaagccattt
     1081 tttggtataa ggtagcatta acaagagata agaatgatac taatggaggt tttaagtcaa
     1141 atgattgtta tggatatata ccatctatac aattaagtgt ctgttatgac aggttaggag
     1201 aatcagataa agctatttat tatcatgaaa aaacaaaaga aatcaaacct aacgatagtg
     1261 ctgttttgca caatgaaaat tattttagta aatttaaaaa tagttgatag gtcacaatca
     1321 tactaaattt gacatattat atatagagct taaaagctca aatatatcag gaggtgcttt
     1381 taataatgag tagaaataaa tattttggac catttgatga taatgattac aacaatggct
     1441 atgataaata tgatgattgt aacaatggtc gtgatgatta taatagctgt gattgccatc
     1501 attgctgtcc accatcatgt gtaggtccaa caggcccaat gggtccaaga ggtagaaccg
     1561 gcccaacagg tccaacgggt ccaacaggtc caggagtagg ggcaacaggc ccaacaggac
     1621 caaccggtcc gactggtcca acaggaaata cagggaatac aggagcaaca ggattaagag
     1681 gtccaacagg agcaacaggg gcaacaggcc caacaggagc tatagggttt ggagtaacag
     1741 gtccaacagg cccaacagga gcgacaggag caacaggagc agatggagta acaggtccaa
     1801 caggtccaac gggagcaaca ggagcagatg gaataacagg tccaacagga gcaacagggg
     1861 caacaggatt tggagtaaca ggtccaacag gcccaacagg agcaacagga gtaggagtaa
     1921 caggagcaac aggattaata ggtccaacag gagcaacagg aacacctgga gcgacaggcc
     1981 caacaggagc agtaggagca acaggaatag gaataacagg tccaacaggt ccaacaggag
     2041 caacaggagc agacggagca acaggagtaa caggtccaac aggggcaaca ggagcagatg
     2101 gagtaacagg tccaacaggg gcaacaggag caacaggatt tggagtgaca ggcccaacag
     2161 gtccaacagg agcaacagga ataggaataa caggagcaac agggttaata ggtccaaccg
     2221 gagcaacggg agcaacagga gcaacaggcc caacaggagt aacaggggca acaggagcag
     2281 caggactaat aggaccaacc ggggcaacag gagcaaccgg agcagatgga gcaaccggag
     2341 cgacaggggc aacaggagca acaggtccaa caggagcaga tggattagta ggtccaacag
     2401 gagcaacagg agcaacagga gcagatggat tagtaggccc aacaggtcca acaggggcaa
     2461 caggagtagg aataacagga gcaacaggag caacaggagc gacaggtcca acaggggcag
     2521 atggattagt aggtccaaca ggagcgacgg gagcaacagg agcagatgga gtagcaggtc
     2581 caacaggagc aacaggagca acaggaaata caggagcaga tggagcaaca ggtccaacag
     2641 gggcaacagg tccaacagga gcagacggat tagtaggtcc aacaggagca acaggagcaa
     2701 caggattagc aggagcaaca ggagcaacag gcccaatagg agcaacaggt ccaacaggag
     2761 cagatggagc aacaggggca acaggagcaa caggtccaac aggtccaaca ggggcagatg
     2821 gattagtagg tccaacagga gcaaccggag caaccggagc gaccggagca acaggtccaa
     2881 caggagcaac aggagcaaca ggagcaacag gtccaacagg tgctagtgca ataatacctt
     2941 ttgcatcagg tataccacta tcacttacaa ctatagctgg aggattagta ggtacaccag
     3001 gttttgttgg ctttggtagt tcagctccag gattaagtat agttggtgga gtaatagacc
     3061 ttacaaacgc agcaggaaca ttgactaact ttgcattttc aatgccaaga gctggaacaa
     3121 taacatctat ttcagcatac ttcagtacaa cagcagcact ttcacttgtt ggctcaacag
     3181 ttacaattac agcaacactt taccaatcta ctgcaccaaa taactcattt acagctgtac
     3241 caggagcgac agttacacta gctccaccac ttacaggaat attatcagtt ggttcaattt
     3301 ctagtggaat tgtaacagga ttaaatatag cagcaacagc agaaactcga ttcttactag
     3361 tatttactgc aacagcttca ggtctttcat tagttaatac tgtagcagga tatgcaagtg
     3421 caggaattgc aataaattag attagataag aaaaggcttc taatatcgta ttgatattag
     3481 gagctttttt aagaatgatt gatgaacata gttttaatat taattagtgc gcatattata
     3541 aaatactatt atataatata aaataataac attaaattgg aaatatagaa ttatctaggg
     3601 gtgatactat gaaaaataag attgaaatat caatgaacaa ctttgtaaaa aattatcata
     3661 aatcaaataa tataactaca aagtggcaag aacttttgtt tgcttatgca tctgctgaag
     3721 atgatatgtt tcagaagcta aaaagtgttg taggaacatc tcatgctatg ccaaaagatt
     3781 ttctggaaaa tggaaaaact ataattacat attttatacc ttttgatgaa agtgtaaata
     3841 atagtaatat tggagggttt gaatgttctg aaatatgggc aagagcgtac atagaaacta
     3901 atatgttgat ttcaaattta aatgatttct tatgtgaaga actagagaag atgggggcta
     3961 attcatatgc tattcctggg tcgtataatt ttgacagtga aaaattggta agtgactggt
     4021 cacaaagaca tgtagcattc atagcaggtc ttggaacttt tggtttaaat aatatgctta
     4081 ttacagaaaa agggtgttgt ggaagaatag gaagcataat cacaagttta gaattagagc
     4141 caactaaaag accagaaaat gaatactgct tgtataaaca taataatagt tgcaaaaaat
     4201 gtgtcaacaa atgcgtgaaa gaagctttaa aagaagatgg atttaatagg aataagtgtt
     4261 atgaaatgtg tatgataaat gaaaaaaaat acacttatat aggtggatgt gatatttgtg
     4321 gaaagtgtat agttggagtt ccttgttcag taataaaccc atcaagataa tggatttaag
     4381 ttgactaata attttaaaga tagtttataa tgaaaaaatt ttagttatca aaataaatat
     4441 aatataaaaa ctaaataaat gtgtttaaaa aatggataaa taatttgtgt tgattgacaa
     4501 ccagttatac tgttgataga cttatatgta tcgctgtgat atataaggag ggttgagatt
     4561 ttatgtttga tttaatcttt atttttgcat agcaagggct attgcaacta tgaaatttaa
     4621 tatataaata gccctttgct attcctaatg aaaaattact atttaatagg agagcaaaat
     4681 gagctataaa aaagcaatac atatattacc agaagaatta cttgaattaa tacaggaata
     4741 tgtagatggt gaatgtatct atattcctag aaaatctaat aataaaaaag aatggggaag
     4801 taatacacct actcgtgagg agttgtcaat tagaaatatg cagatttacg aagactatca
     4861 agttggatat gatttgcaat atttatcgga aaagtattat ttatctttga aaagtatcca
     4921 aagaattgta ttacaagaaa aaagaaaaga tatctaagga tgagccagta tagttttata
     4981 attatactgg ctcattttct tttgataaaa gtgtttgagg tatagtttct tttaagtgga
     5041 tgatataatt taagtaattt caataactta tacagtagct aacaaagctg gttgtaaagt
     5101 atctaataag aagtagtagc tttttatatg tttgaaatta aaatttatta aaattaggag
     5161 gtcttgtttt gaaaaaaata cttgattata ttccaccttt tcataacttt ctattaattt
     5221 agtaatctta tagaaaggaa attgcattta tgaaaaaaat tacttgggag gtggaatata
     5281 tctcccagtt acctgtaatt aggtattgta aaaaatgtgg aaaaaagact gaatatattt
     5341 gttcaggttt atttagggtt aatgctcaac ataaatattt ggatatatgg ttaatataca
     5401 agtgttcaaa ttgtgattca acatggaact caactatata ttctcgtgtt aatccaaata
     5461 gattaagtac agaaacacta gaacaattcc atacaaatga tgataatctt gctaatcaat
     5521 atgctatgaa tgtagaactt ttacatagaa atggagcaga ggttggtatt ccagaatata
     5581 aaatagtagg acaagaaatt gatattaatg accttacaca agttcgtatt gtaagtaagt
     5641 atccatccca attaaaggtg tcagcattat tacgtgaaaa actaggttta tctaggaaag
     5701 catttgatga aatgttagat tgtgggacta ttaaaagtat atctggagtt gatttaagga
     5761 gaagtaaatt aaattatgaa attgtgttag aggtaggcaa gcagaagcaa aagtcttcaa
     5821 actgattaga ataactaact taatatattt tcaggaacat catttaaaaa aatagtgact
     5881 ttaaaaaatt taaagccact attttttaat atgttttata tgagtattta aaataaagtg
     5941 tgtattcttc aaatttatag cgataattaa aacattacta taaaaaggaa ggtgcatact
     6001 ttttatttaa caaaaactag atattaaaaa cttttcgagg aatattttat taaaatccct
     6061 aaatatattt aatatatatt aaaaaattgt attataatga aaatcaaaat agattgcaaa
     6121 tccaaaaagt aatacttaat gtccaaaaag ctaaaattaa aatctaaata gctatttact
     6181 acagaagtat gttaaaatat gtaaaatgaa tagaaattta tatattttta ttaaaaatat
     6241 ataagaaagg atgtttaaga attgtgatta atataggaat atgtgatgat gaactacatt
     6301 atcgaataaa aataaaagat atattaagtg aaatattaag ttcatatcct ataaattata
     6361 acatatatga attttcagca ggagaagagt tactaaataa ctatcctaaa gatttagaca
     6421 tattaattat ggacatacaa atgaaaacaa taaatggtat ggatactgct aggaagataa
     6481 gagagtttga tcataagtta gaaataatat ttgttacttc atttgtagag tttatgcaag
     6541 aaggttatga ggtaaaagca tacagatata tactaaagcc aataaataaa gaaaaaattt
     6601 ctaaaagtgt attaccttgt ataaatgaga tgatgaaaaa gagaaataat tacttaacta
     6661 taaatgtaaa aaattatgta gaccgaatta aaatagattc tattacttat atagaaacag
     6721 atagaccgaa tatattgatt tatacacatg atgatatgta tattactaaa atgagtatat
     6781 ctaaaataga aaagatatta aatgaatatg gattttttag atgtcataat agctatatag
     6841 ttaacttaaa attagtagaa tcaatgagtg gaagtactgt tatagtagat ggaaaaagta
     6901 ttccaataag taaatatagg gttaaaggct tgaagcttgc gataactaat atattagggg
     6961 acatagtatg ctaacaaatt taagtgtagg attttggaat attattaatt tagtttcagg
     7021 tactatagat tggattatat tttttttagt aatccactta gttggaaaga ggaaaatatc
     7081 taatataaga tatatattta caacaataat tataattata tttatggcat acataaatgt
     7141 agttaaagtg tttccaaata ttaagattct tctatgtatg gtaataggaa tatcatttta
     7201 tataacatca tatgaagata aaatctataa aagtataata gttagtttat tgttttggct
     7261 aggattaatg gttgctgaag gttctgcagt aggtctagta gtatttataa ataaattaaa
     7321 taatattaac ataacacaac atgaaaactt atttagaata caagctatta ttatgtcaaa
     7381 ggtattttta tttataggtt ttatattgtt taagtatttt aaattatctt tagattttaa
     7441 acctaaagat atgatactaa taggaatacc tatattatct aatatagtta gcttattact
     7501 tatatttgaa tataatttaa gagtcaatac aattactaca gaaaacttat ttatatttat
     7561 atttgcaatt ttattgattt tatcatcaag tataatactt ttaatagtaa tagggaaaat
     7621 tgtacaagac gataaattaa atctagagta tgaacttata aatgaaagaa taaaaacaaa
     7681 ctacaggaat tatgaaaata taaacgaagt tcatgataga ttaaaatatg tttatcatga
     7741 cttgaaaaac catatgattt gtataaaaaa ttatgacact aaagaagaaa ttatatcata
     7801 tataaataac ttagagtttc agataagtga atttgaacat tttaaaaata ctggaaataa
     7861 aacattagat ttaattttag cagaaaaaat aagtatttgt aaaaagtatg atattcaaat
     7921 tgaagataac ataaatatat ctaaattaaa ctttattaaa aataatgata tatgttcaat
     7981 atttgcaaat tcactagata atgcaattga agcatgtatg gatataaata atgagataga
     8041 aaaaagaata gaagttaaag ctacatatat aaataaattt gcaataataa aatttataaa
     8101 tactaaggta aatgatatta aacttattga taaacgtatt caaactagta aggatgatga
     8161 taatatacat ggaataggac ttgctagtat taaatatata gtaaataaat atggtggtga
     8221 aacaatagtt aattactcag ataatgagtt tatattaaaa ataatgatac caataaaaag
     8281 ttaggagagt acaaaatgaa aaaaatagcg ttaaacttac ttaaaaacat ctcagcatta
     8341 agttttggga tagctgtatt atcagcaaac agtgcaagtt cttgggtagc acatcaggca
     8401 aaagagccac aagctttaca aaaactaaaa aaataacatt aataaaagga cctcttctta
     8461 ttaaaggaga ggttttacat gttgaggagg aaatatgttt aaaagattat catataaatt
     8521 tgccaatata ttagttaata atgagattgt agaaagtgaa gattttgaaa tatatagata
     8581 tggatttgaa actttgattt atttcatagt taatattttg gtagcattat ttataggaat
     8641 tatttttgat agatttatac atacagttat atttttaagt tgttattgca ctttaagaca
     8701 gttcacagga ggttatcatg ctaggaatta taaagagtgt acattaacat ttgtagttat
     8761 ttatttgata actatttttt cagctaataa tatagatata aataaatata agtatttatt
     8821 agtattatta atgattatta gtatactaac aatttacaag ttagctcctt tagaacatag
     8881 aaacaaacct cttagtgaaa gtgagaaaaa gcattataga aagactgtgc aaaaaatact
     8941 attcgtaata atatgtttaa ttattttatg taaaatactt aatatttttc agcaatatgt
     9001 gatttatgca ataatttcta tatattggat tgctatatta atatatattg gaatgaaggt
     9061 taatagtgat cagtaagatt ataaaacatt ttctaaatta attaacttca ctaattaata
     9121 ttaactttaa aaaaataatg tcaaggtaag tatttgaact tattaaatat agttgaatct
     9181 attgtgggtt gtatcaaagg acaaatagat tcacttaata accagtttgt aacagagtat
     9241 tgaattgtaa aagttaatac gatagtatga aaatgtaatt tgaaattaca tttttataaa
     9301 aattatatta actaagggga gggatacata caatgaattt gaagaaaaaa attaaaaagt
     9361 taggagttac atttgcaata ggaactataa ttgtatcaag tttagggaca tctgtatttg
     9421 cagatgaggt tagcacatat gctttaatat ctaaagcttt ttcaggtaaa aacaatatta
     9481 tatctagttc agtatacact tctggaagta gaacctttaa aacgacagat tctcaagggg
     9541 gattttcttg taggctaaca gaaagattct caggatctag cccagataga attgtaaaaa
     9601 gacatgaagc aaatgcttct tcaaaagtta gtttatcagg aggaacatac tatataaggt
     9661 atgaaggttc aacgaataat gctaaaacat atgcaacttt aaattagtat taaaagcatc
     9721 ctataattat ttttatagga tgtttttgta attgttataa tcaaaagact ggctaggagg
     9781 ggcataaata tgagtagtat taaggttata tcagacttta taaccaaaat aattagaaaa
     9841 aatattttat ttgcaataac aattattatt ataaattacc atctcataca acgatggaca
     9901 catatatcca atagaggaat ttcatttaat gattacttat ccttgagtgt atatacttgt
     9961 atgacaatta gtattttaac catgttaaat gtaatttttt atatgagaaa aagttatgat
    10021 actgtaaaaa tattatgtag aaataatttt gattattttt taggtatttc tgttgctgta
    10081 actagaaaca actttatatt ttgttgcata ccaattatat atgctatagc atactcaagt
    10141 ttaataagaa cattgtcaat tgtaacgatt aaatcaacta tatttatatt gatatttgaa
    10201 tggtttttac cacttactat tttatctata gtgatatcat ttatttcagt tattataaat
    10261 aatttaatac taaagattac cttatcatca gtatttttat atttaacatc aactaatatg
    10321 ttggatagag ttattgatat taaagaacca ttttttaaag cattatttaa atcacttaac
    10381 atatttgaag atcaatcata tgcttactat tctgattttg tagggaaagt taataattca
    10441 gcatatgtta tagataagat tatacctaca ctatttgcaa ttactttaat aatagtagca
    10501 tatctgatat taaatgatat tagaaagaaa cagattaaat cattaatagt tatagtagtg
    10561 tacagtattt tatttattgg attaaattat atatcaatag atatgtcaac atatagagat
    10621 gaggagcgta gttatggaga gtatttaaag aaatctaata ttagtgatat taaaataaaa
    10681 tcgcatgata tgaagattga ctttaaaagc aagtcaaata taaaagatag aattgtttta
    10741 ttaaacacat caaatgaaga gttaacaaat attaatatga ttttagatga aatatttgat
    10801 ataaaatata taaaaataaa ttcggaaaat ataaaattta acaaaaaaaa ggatttaatt
    10861 gaagttaatt taaaggaaag tttagagcct aatgacgagg tgacattaga tatagagtat
    10921 gaaggctata ttaatgtgtt gacatcttgg aatcaaaata aatatatagc tactaaagat
    10981 gatattatgc ttccaccagg ttctttagcg tggtacccaa aagtaaatca atcaagttgt
    11041 atggcttata aaataaattt aagttctaaa aataatgtct attcaaattt acctttactg
    11101 aataagacta gtaatttttt aaaaaaagat tatatatttg aaggaaagtc aaatgatatt
    11161 gcaatatatg caggtgagtt taaagaagag gtaaaagatg atataaaaat aatctatcca
    11221 tcagatagta atgtaaatat ggatatagag gaaaaaatta aatttttaat tgaaacaaga
    11281 aatgaaatat taggtgatag caaagcatct gtagatgcta aaaaatatgt agatgaagta
    11341 gataatgtta tggaatcaat aaaaagaaaa aaaattaata tgatattagt tgctgactta
    11401 cctagcaatg aatttttata tgaagactca aagggagaga aaattagcat aactatttat
    11461 cattggcttt caggaaatat ccttataata gataaataat aaaggaggtt aatatgataa
    11521 acaagttaga aataagaaat ctaaatttta attacaaaaa taaaaaagct ctggataata
    11581 taaatttaac cctaacagat ggagtagttg cattattagg tcctaatggt gctggaaaat
    11641 caacattaat gaggttatta gtgactcttt atgaaacttc aattggagag atagaattaa
    11701 ataatataaa gtatagcaaa aacaatgaga aaatcaaggc aaatgtagga tatgtacctc
    11761 aagattttga tatgtataat aatataaatg ggcaagaata cttagaattt gtagctaaaa
    11821 tgagaggtgt atctaagaat gatttaaaga aacatataca aaaagtagta tcaaaagtta
    11881 atctggacaa gtttataaat aaaaaaatag gtacatactc aggtggtgta aagagacgac
    11941 taggtatagc acaggcttta ataggtgatt ctaagttaat agttatggac gaacctacag
    12001 ttggtcttga tccagagcaa aggaatgaat ttagaagatt gttaccagaa ataagtaaaa
    12061 attctatagt attaatttca actcacatag tagaagacat acaatttaac tgtgacaaat
    12121 taattatatt aaatcaaggc aaaatattat atgatggtac aattaataag tttattgata
    12181 tggttgaggt gtattcggta gtagtttcta atgatgaatt tgaatattta gaacgtaata
    12241 ttgatattat tgattatagg agagttaaag atggtgtgca agtaaagtat ataggtaaaa
    12301 atacagagaa acttaaagat agcttaagga tagatacaac tttacaagat gcttatataa
    12361 actttttaca taaggagtta aaataaatgt gtatattgga gttaataaaa tttgagctta
    12421 aaaaaataga gcttaaacat gtgttaatat ttgtaattat ttctatatta tgcatattta
    12481 ttacaggcat attagattat aatgagttaa tattttataa tgatgtacct ataatggata
    12541 catattctat agcaggatgg ttaggaatta taaagatatc agatgttttt ataccaataa
    12601 tgtcagcaat cacaataatt tatgtgtttt caaaagatta ttcgggtaat ataaatgaga
    12661 tacttacttt atataataaa aaaaaatata atcaatttat agtatttaga tggtgtgtta
    12721 tattagctat atatactgta ttattattaa tttcaattac aatagtatat acatttacgt
    12781 cagtgaaagg ttttaacata gacaaaatag attttgttta tagtgtaaag ccaatagatg
    12841 tgtttttaaa gacgtttcct acattattat ggtatacaac atttccacta atgatattaa
    12901 cattaacaaa aaatagattt acaacaatag caatattaat tacatataca tttgctgata
    12961 tattttttat tttacatatg tacccatttg ttagtatgat taatgtaaat ggattttata
    13021 ttcaaaagat gttttatatg cctaacaata tagatgttag atttaatgaa ttaaagaatt
    13081 atttctttat aaatagaggt tctctagtag ttatttctat aataatagtt ttttatatag
    13141 caagaaaatc aacggtttta aaaaagagta aatagtttaa tatatttttt atattgttat
    13201 atttatatag aatagtgact ttaaaaaatt taaagccact attttttaat atgttttatc
    13261 tatataacta ttatttttct aggtttttta atatttcgta atagtgtgtt gtggctattt
    13321 cacttgactc gcctttttta caaaaagata atttagatag ttttggaact acacgttcaa
    13381 cgacaatttt attacctatc attttagatt caagtatctt caatgcttct aaaaatctat
    13441 tatccttttt tgcacgatta taaaatgata aaacgtagac ataaaggaat aggttataat
    13501 tacggaaagg atattcaact tgcataaata atgtgccaat tccatagtga caagggccga
    13561 ttggtttttt aatagtccaa tggtcaagta gaaaatctac agctttgtca agttttgatt
    13621 ctttatttag ataattacta aaacggaatg catttaatat attaagagtt ggtagtggat
    13681 ttgagtattc agtttctgga cctcgaccaa aactaaattt attgcaacgc cacccaccat
    13741 cagtatactg tgtatctaaa aaatgttgaa acgttttttg cattttaatg tcagatgcat
    13801 atcccatatg acagagtacg ttagcagcat ttgcagtatg acaagggtaa atacctccat
    13861 ttggatatag tttgaaacgt ccatcttctc gccatgtgct aaatatcagg tcagcacatt
    13921 ctttaagtaa agtatcatca gggttcacac ctaattctaa tagataaagt acactatcaa
    13981 gagtagagaa aggagaacct tttattaaac gcttatcagg tgtagtccaa tagtctgcac
    14041 cattatcata cctgtgtgat aatattattt caatatctga taaagattga ttttctacat
    14101 tcatttttta ctccatttca tattatattc tattaaatct atcaaaaact ttattataat
    14161 attatttata tttataattg atttatttta tttaaaattg catttatagc tgtatcagga
    14221 acaactgcta aatcttctgc attgaagtaa taaactatat tagaatcaaa taatatattt
    14281 gaatttggag gaaattcatc atctccaaac caaagtataa ataccataga tgtattaccc
    14341 atatatgtaa acctataagc taaatctccc atgtttactt tttcagctcc aattttctcc
    14401 ataactaact tatatttgtc tatttgagat gtaaaaactt ctgaaagcct atatagagtc
    14461 ctgtttaaaa aatttggata ataaacattg cctcctggta tttctttaaa agttacatat
    14521 ttattagttc tagggatacc tttagcattg attaaatatc taactatcaa gattttaagc
    14581 tcaaagttat tgcaaaggct accattacta ttccaaactt ctccacttgg atagtctata
    14641 atatattgtc tatcaaaaaa attgagtgtg aatctattta aatcattatt gaaaggtata
    14701 ttacttaaat tactaattgt atgagggtct aattctttga gtttttttcg aacatagtca
    14761 aatggaacat tataattatt ttttgttatc ttttcatcca taattttttc
//
LOCUS       DAELNY010000089        14720 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.089, whole genome shotgun sequence.
ACCESSION   DAELNY010000089 DAELNY010000000
VERSION     DAELNY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.089"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(246..1604)
                     /gene="rlmD"
                     /locus_tag="KPE55_002247"
     CDS             complement(246..1604)
                     /gene="rlmD"
                     /locus_tag="KPE55_002247"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0493310.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(1779..3539)
                     /gene="pyk"
                     /locus_tag="KPE55_002248"
     CDS             complement(1779..3539)
                     /gene="pyk"
                     /locus_tag="KPE55_002248"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG0493311.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(3591..4550)
                     /gene="pfkA"
                     /locus_tag="KPE55_002249"
     CDS             complement(3591..4550)
                     /gene="pfkA"
                     /locus_tag="KPE55_002249"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0493312.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(4759..8328)
                     /locus_tag="KPE55_002250"
     CDS             complement(4759..8328)
                     /locus_tag="KPE55_002250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG0493313.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(8485..9432)
                     /gene="whiA"
                     /locus_tag="KPE55_002251"
     CDS             complement(8485..9432)
                     /gene="whiA"
                     /locus_tag="KPE55_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG0493314.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(9449..9874)
                     /locus_tag="KPE55_002252"
     CDS             complement(9449..9874)
                     /locus_tag="KPE55_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0493315.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(9943..11049)
                     /locus_tag="KPE55_002253"
     CDS             complement(9943..11049)
                     /locus_tag="KPE55_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG0493316.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(11061..11918)
                     /gene="rapZ"
                     /locus_tag="KPE55_002254"
     CDS             complement(11061..11918)
                     /gene="rapZ"
                     /locus_tag="KPE55_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG0493317.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(11949..12662)
                     /locus_tag="KPE55_002255"
     CDS             complement(11949..12662)
                     /locus_tag="KPE55_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0493318.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(12666..13580)
                     /gene="murB"
                     /locus_tag="KPE55_002256"
     CDS             complement(12666..13580)
                     /gene="murB"
                     /locus_tag="KPE55_002256"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG0493319.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(13853..14623)
                     /locus_tag="KPE55_002257"
     CDS             complement(13853..14623)
                     /locus_tag="KPE55_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0493320.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         4392 a         2915 c         1579 g         5834 t
ORIGIN      
        1 aaataatatt caactataga ttatgtaaat tactctatga aaaaaataag ttgtataaaa
       61 tctggctact gtagattgac gatttctttt aaaattacaa ttataataag ttttatagaa
      121 ttaaaaataa aactttagag atttttgata acattgtaga ctaaatttgc caccaaaatc
      181 gctttacagt ggcaaatcgg tggcaaatac aatatattat tagtatataa tctatataaa
      241 cattactatt gtttgattat cttagctaca gtttccacat gtgtagaatg agggaacata
      301 tctacaggtt gtgcctcaac acatttaaat ccgtgaacat ttagataatc taaatccctt
      361 gctaaagtag aaggattaca agaaacatag acaattcttt ccggattcat tgaaacaata
      421 gtctctaata ccttttcgtc acatcccttt ctaggtgggt ctacaactac tacattggca
      481 gtcttaccct ctctatacat cttaggtaca atttcctctg cctttccaac ataaaattca
      541 gcattatgta tattgtttaa tttagcattt attttagcat cttttatggc atcatctata
      601 atttctattc cataaacctt tttagccttt tgtgccaaaa ataaagatat tgtacctatc
      661 ccacagtaaa tatcaaaaac agtatcagta tcatttaaat ttgcatattc aagagcctta
      721 tcatacaata ctttagtttg gacagggttt acttggaaaa atgataatgg tgaaatctca
      781 aacactaagt cacctatata atcgtttatt tttccatctc catatataac tatattttct
      841 cttcctagaa tagcattcgt atctttttga tttacattta caaccaaagt cttaaatcca
      901 ggtatatttt ccttaagcac tgaagctaat tctttaagat atggaagttt gtttccattt
      961 gctactaata caaccattac ttctttagtt gtaaaaccta tttttgttac taaatgccta
     1021 atcaaccccg tgtgagtctt ttcatcatat atacttacct tataggccct tatatatgtt
     1081 tttattattt taacaatttt atcatttatc tcatgttgta ttacgcattt ttctgtcgat
     1141 attacatcat gagtcttctt tttatagaag ccaataattg gaataccatt attcttttgt
     1201 attggaaatt gtgctttatt tctatatcta tatggattat ccattcctat agtgtcatga
     1261 acttctacat cctctaactt acctattctc gaaagtactt gtttaacttc atttgttttc
     1321 atttcaagtt gtttattata atctaagtct tgtatctgac accctccaca atccctaaga
     1381 ttgtcactac acactctttt caccctgaat ggagactttt ctattatctc aacaatatct
     1441 cccacagcat aatttttctt tactttatta atcctaactt taactttatc atgtattaat
     1501 ccgccctcta caaaaactgt aataccattg tactttccta ctcctactcc gccttgacca
     1561 atgtctacta tatctacaat atacatctcg tttttcgaaa gcataaatca ccctccaatc
     1621 aatctattat atttaaatct ttacaaactt ttttctttaa atttatatta aaatattgct
     1681 attaattaaa ctatattttt tactaagcta caatatttta ttttaccaaa aaaaaaggag
     1741 cttataaagc cccttagtaa ataaatttat tatttttctt ataaaactct agttgaacct
     1801 ctatatacca cgcctcttga tgcatccaca gttacaactt ctccatcttt tactagattt
     1861 gttatatcag ctgcagacac aataactggt atatctaaat ttatacctac aatagctgta
     1921 tgtgatgtca ttccaccatt ctcagttact acagctcctg ctttttctat ataattgttc
     1981 atttcagcat cagtagcagt agaaacaagt atgtcgcctt cattaaagtc tacacaagca
     2041 tttccattct ttattatacg aactttacct tctaccgttt gagtacctac acctatacct
     2101 tgcacgattt cttcgcttat tacatgaacc tttattaaat tagtagttcc acttactcct
     2161 actggtactc cagcagttat aactactaat tccccattat ctatataatc agcttgtcta
     2221 gctgcctcaa ttgccttttc tataacttca tctgtatttc ctgcaacaga agacttaact
     2281 ggatatactc cccatgtaag agctaattta ttcatagtct tttcattatt tgtagtagct
     2341 attattggag attttggtct aaatttagat accattctag cagtatatcc tgaagaagta
     2401 gatgttatta tagctgatgc atttaaatct actgctgttg tacaagttgc atggcttatt
     2461 gcatctgtta cagtaacatt gttagtacca ttttccttta gtaatctatc ataatctaaa
     2521 gtctcttctg ttctttttgc tattgtagcc atcattttta ctgcctctac aggatatttt
     2581 ccagcagcag tttctccaga taacattatc gcatctgttc catcatatat agcattggca
     2641 acgtctgtaa cttctgctct tgttggtctt ggatttctta tcatagaatc aagcatttga
     2701 gtggcagtaa caactggctt tgctaattca ttacattttt ttatcatcat tttttgaact
     2761 attggcatct cttcagttgg tatctcaaca cctaagtcac ctctagccac cattatacca
     2821 tcagaaactt ttaatatttc atctaggttc tctacacctt cttggttttc tattttagat
     2881 attatttgta tatcagttgc attattgttc tctaatactt ctcttattgc tagtacatca
     2941 gatgcctttc ttacaaaaga agcagctata taatctattc cttgacttat tccaaactct
     3001 atatcactta tatctttagg agttattgct ggtaaattta tcttaacacc aggtacattt
     3061 actcctttat gatttttaac tattcctgaa ttttcaacaa cacaaactat atcttctcca
     3121 tttatttctt ttactcttaa gccaactaaa ccatcatcta ttagtataga atctcctgat
     3181 ttaacgtctt cagcaagacc tttatagctt actgtacaca tttctttagt ccccataata
     3241 tctttcatag ttatagtgaa cttttgccct tcttctaaaa atacctctgg gtcttcaaag
     3301 ttacctgttc taatttctgg acctttagta tctaataata tagcaactgg cttatttaat
     3361 ttttctctaa ctttctttgc catatcaatt ctttctttat gttcttcatg cgaaccgtga
     3421 gaaaagttaa atctacacac atttagacca ttttgcataa gttgagttaa aacttcctca
     3481 ctttgtgatg ctggacctag tgtacaaaca atttttgtct tctttacatt atttaacata
     3541 tttgctcctc ctgagatatt attccacaga tttattttta atactaaaac ttatatagat
     3601 aatatttttg ccatctcata agcttcttta tcaaatactt tcttttgagc taaagcttca
     3661 cttatttcta aatcaattat tttgttttct tttattccta caactctagc tgattttcca
     3721 tctaataata attctactgc tcttactcca agtctacttg ctagtattct atcagataca
     3781 gttgggcttc ctcctctttg gacatgcccc aatacagtga ctcttaaatc tgctcctgac
     3841 tcttttttaa gttccttttc taaatcacta gcacttccga ctccttctgc taaaacgatt
     3901 atactatgtc tcttacctct tttttgagta gtctttaatc ttagagcaac ttcatctact
     3961 gtaatttcta cttctggtac tattatagtt tccgctcctc ctgcaagacc tgcatataag
     4021 gctaaatctc cacagtgtct tcccataact tctactatgt taactctttc atgagatgat
     4081 gatgtatctc ttatcttacc tatagcatct ataatagtat tcattgcagt atcaaatcct
     4141 atagtataat ctgtatatgc taaatcgtta tctatagttc ctggtatgcc tatagctggg
     4201 aatcctaagt cactaagttt ttgggctcct gcaaatgaac catctccacc aattacaact
     4261 aagcaatcaa ttttatactt tttaagaatt tttactgctt ttaatctacc ttcttctgtt
     4321 ttaaattctt cacatctgga agattttaat atagttcctc ccctgtgtat aatatctcca
     4381 acagaagata agttcatttc agttaaatct tcttctagta atcccttata tcctctattg
     4441 attccatata ctttgcatcc ataatatatt gctgatctta caaccgctct aatcgcagca
     4501 ttcattcctg gagcatctcc tccacttgtt agtaatccta tagttttcat tttttacctc
     4561 cttggatttt atgtcaattt tttattataa tcattgttat tgtattctaa tggacttttt
     4621 ttgtccctat atcacttttt aatcccatca agactaaaaa aattaaatgt ccttaaatgt
     4681 ccattttgtg atattatatc acaaaacgga caatattttt catattatta taatatattt
     4741 agatttttat ataaaaaatc aagcttttac ttttatgtta tctttaggaa gtaactcttc
     4801 taagttattt ataaattcat cacatataca agtcaatgcc ccggtcattt tatatgcttt
     4861 acgtttacca tttatatttt catccatagg gtacaagtat atactgtcat tacctggata
     4921 ttgtttggct atttctacaa tattattagt tgtatcagta tcagacatac tatctatttt
     4981 caaatacaat tttttaccac tttctggctt aatattttct acatttctag ttcttcctga
     5041 attgtagttt ctatctgcaa agtcagtatc atcatttata tcttttatct ctctagctat
     5101 aagttttgca ttttcgcctt cttttatact taatgttcct tttacatata ttatattatc
     5161 ttcattcaaa atgacattat acttttgtaa taattgagga aaaactatta tttctatagc
     5221 accatacaaa tcttctaatt caataaatgc cataatttca tttcgttttg ttgttttaat
     5281 agttttagtt acaatcattc cacccattat aacatctgtt tcattcattt gtaggtatga
     5341 ctcttcttcc tcttttaatg aatttaattt tccattatcc atagatgttt tttcttgtag
     5401 ttctttttca aattgtgata aaggatgtcc acttacatac attcccaaga cttctttttc
     5461 aaggttaagt ctctctcttt cttcgtactc ttgcctttta ggcaaactgt atatttgttg
     5521 aaaatcacta tcttcctcac tatctccaaa agcatcaaat aaagaaattt gaccttgaac
     5581 atttttcttt ctatccatag atacactttc taataaactt tcataaccag ccatcagact
     5641 agctctattt tcacttatat tatcaaaagc tccacatttt attagacttt ctattactct
     5701 cttatttgta tctttttggt ctaatctttt gactaaatct actaaatcta caaataatcc
     5761 attagattct ctttcagcga ttatattgtt tattatattt acgccaacat tttttacggc
     5821 tgctagacca aatcttatat tgtcgccttc aactgaaaac tttgaaaaac ttttgtttat
     5881 atctggaggt aatacatcta tttctagtgc tttacattct cttatatact caactacctt
     5941 atcagtattt cccattacac tggttatcaa tgctgccata aattcaactg gataatatgt
     6001 ttttagataa gctgtctcat atgctaaaac cccatatgca gctgcatgtg atttattaaa
     6061 tgcatatcta gcgaagtcta tcatatcatc aaatatttta ttagctattt cctcttctac
     6121 accatttctg atacaccctg caatttctat attaccatct tcatcaaatt taccatgaat
     6181 aaaatattgt ctttcttctt ccatcacgtc catctttttc ttactcatgg ctcttctaac
     6241 taggtcactt cgtccgtaac tataaccacc taaatctcta acgacctgca taacctgctc
     6301 ttgatatacc aaacaaccat atgtaacttc cattataggc tttaattttt catgtagata
     6361 tgttacatct tttgggttgc ttttattttt tatatatgca ggtatagaat ccattggacc
     6421 tggtctaaat agggatattc ccgctacaat atcttcaaaa ttgtctggtt ttagctgttt
     6481 cataaaactt ctcatgccag cactctccaa ttgaaacacc cctagtgtat ttcctgatga
     6541 aagcatttca tatactttag ggtcgtcata atccatcttt gaaaagtcta ttttttctgt
     6601 gtagtttttt ataactctat ttttttctat taaatcaaga gtatctctta taactgtaag
     6661 agttcttagc ccaagaaaat ccatctttaa aagcccaagt tcttcaagtg ttgtcatagt
     6721 aaactgtgtt gtgattgcat cttgatgttt gtacaaaggt acatattcat ctacgggatt
     6781 ttttgatata acaactcctg ctgcatgagt agaagcatgt cttagcattc cctctatctg
     6841 ttttgatata tttattattt cttttgtttc cgggtcttgg tcatacaatt ctttaagatt
     6901 aggatttgta tctagggcct tatctattgt cattcctaaa gcaaatggta tttccttggc
     6961 taccttgtca actttattgt agcctatatc caataccctt ccaacatctc ttattgcagc
     7021 ttttgcaccc attgttccaa aagttattat ctgggctaca tggtcatcac catattttct
     7081 ctttacataa tctataactt cttctcttct ctcataacaa aaatctatat ctatatctgg
     7141 catggatata cgttctggat ttaaaaaacg ctcaaataat agagagtact ttattgggtc
     7201 tatatcagtt attttaagtg tatatgctac tatcgaacca gccgcactac ctctacctgg
     7261 tcctactata atattatttt ctttagaaaa atttataaaa tcccaaacaa ttaaaaaata
     7321 ttctacataa cccatttttt caattacatc taattcataa tttaatctct ctagtatctc
     7381 attgctagga ttatcatatc tctcttttaa tccattaaaa cacaactccc ttaaataagt
     7441 ttctggtgta tatccttctg gaacatcata cttaggaaga tgtatagtgt taaaatcaaa
     7501 ctccacatta cacatatcag ctattttcac agtattatca atagcctcta atgcatatgg
     7561 aaataattct tccatttctt ctcttgattt taaataaaac tcatcactac caaatctcat
     7621 tctatttggg tcatttaatg tttttcccat ttgaatacac ataagtacat catgtatttt
     7681 cgaatcttct ttatttacat agtgaacatc attagtagca actagtggta taccagtctc
     7741 ttttgataat tttactaaac cagagtttac ttctttttgc tcaggtaaat tgtgatcttg
     7801 aatctccaaa aagaaatttt cttctccaaa tatgtctcta tagtcaagag caaattgttt
     7861 tgctttttca taatttctat tcattaaagc acgtgctaca tctcctgcaa ggcatgcaga
     7921 aagtgctata atccctttgt tatgcttttt aagttcttcc atgtcaactc taggcttata
     7981 gtaaaaacct tctacgtatg aagttgaaac tatttttatt aaatttttat atccttccat
     8041 attcttagct aacagaacta aatgtccttg atatttgtca tagtttgggt ctttatctct
     8101 caatccacgt gcagctgtat agacttcgca ccctataatt ggctttattc cttcttttat
     8161 agctgttttg tagaaatcta taacaccaaa catacaacca tggtcagtta tagctattga
     8221 gctcatgttt agttccttag ccctttttat aagttttttc accctcgaaa acccatcaag
     8281 taaactatac tcagtatgta catgaagatg agcaaaatct ttgttcatta aatcacctcc
     8341 tgctttataa ttttaatttt accacaaata tatgtactaa aacaaaaaca gagactttta
     8401 tctcaaaata ggaattaact tcaacgtata ccattatatg ttataaatta atgcttattc
     8461 tgagattgtc ccctcaattt attgctatga tttttttttc aaatctgttg caatttcttc
     8521 tatttttcta agtctatgat tgactccaga ttttcctatt ggtggttcta acatctctcc
     8581 aagttctttg agagtcatat cctcatactc gattctaatc ttagctatct cttgtaaatt
     8641 ttctggcaaa ctgctaattc ctatagtctc ttgtatgagc ttaatatttt ctacttgtct
     8701 tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta tcctgtttac
     8761 attatttctc atttctttta ctattttagt attttgaaga cttaaaagag cgtggtgtcc
     8821 tcctataata ctcaacaaat ctgatatttg ttcactctct ttgatataaa caacgtgatt
     8881 attttttcta gccacaattt tactattaaa acctaaagag tttataagtt cttttagaga
     8941 atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt ctgggtcact
     9001 tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc cttcatgctt
     9061 aattaagttc tctgaaattt tgtttatagt aaaaaatcct tctttatctt caagtatacc
     9121 taactttttt aataacattt ctgaacccat ttcgcttgtt accattaata cataactgtt
     9181 atttctcttt agcatttgat tcttatttac tgagattgta gtattgatat taaaattctt
     9241 ttttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta tctttagatt
     9301 tagcttctta tatccaacaa tttgtatact tccccctgat ttaactagag ctgacaattc
     9361 agctatatta caacattcat tcccagatat aactcttgct agttcatttt tggtttcagt
     9421 tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt tttatttctt
     9481 gtaaggcaac ctttattatc tccttttcat catcatacct agctaagtct actactctat
     9541 ctaggtggat aaatttagca tcaataaagc cttcctcttt ttgtggaata gtatccatat
     9601 tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt tcatcccagt
     9661 tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct tttactccag
     9721 tttcttcact tacttcacgt aatgctgcct cttggttatt ttccccttct tctacttttc
     9781 ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaatcgta ttgccaaaca
     9841 ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta aaactaacat
     9901 aatgataagg atttctacac aattttttaa aatccttttt tactaacttt tgttgtaatt
     9961 atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat gtcttatata
    10021 attatttttt atttcaatta aattcttctc tacagttttt atacccatct cttttaattt
    10081 atctttttgc tttttatcta acaagacttg ctctgctcca tcttgtttat ataagtcaag
    10141 catttcttct ggcaatatct cattatttgc gattacataa tctattaaat tctcatcagt
    10201 gtgttttact atagcattaa catgctctaa tacattatac ccttctgtct cacctggttg
    10261 agtcattata tttgctatat atacttttgg agctacagac gatttaatag cgtctacaat
    10321 cccctctaca agtaaattgg gtattatact tgtatataaa cttccaggac ccataattat
    10381 aatgtcagca tcataaatag atgcaataac atcctttaat ggttttacat cttttggatt
    10441 taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta tactagattc
    10501 tccatttatt atattgccat tttctaattt ggcaactaaa tttacatctt ccaaagttac
    10561 tggtaaaact cttcctgtta tagcaaaaat ttcacttaat ttatatacag ctttttcaaa
    10621 gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct gaccttttaa
    10681 aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta tatttgcaag
    10741 tgctaataaa cagtttctta tgtctcctgg tgggagcatt cctaagtctt ctctcaatac
    10801 acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt gcgtagtgtg
    10861 tttaagacct cttaaaaata ccgattgacc agtacctcca cctatcacaa ccacatttgg
    10921 ttctctattt tcaacaatag acctttgaaa tagttttact ttttcatttt ttgctttttt
    10981 atatataaat aacgatacaa ttaatgcaat ccaacctaaa attcctacaa taataagtaa
    11041 tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt tacaactcta
    11101 tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga cctatgtctt
    11161 ccacctgtgc atccaacacc aattactaga tgttgttttc cttcctctat atattgaggt
    11221 actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat tttagaattc
    11281 ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc ttcaacataa
    11341 tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag tataccatgt
    11401 ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc agaagaatat
    11461 attttcttta tttcttcctt aaggtcttta ggtttcatgt tagttgtatc aattatacat
    11521 gtagataact cctttaaagg ttccattatc tttctttcca tttttatacc ttctggaatc
    11581 tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt taacaataca
    11641 tcatcattac aatccaaata taccatttca tattcataat tttctttttc taaataattt
    11701 aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc tagagcaact
    11761 ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat caaagcagga
    11821 ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt ctcacttttt
    11881 ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc ccttttatct
    11941 tatgtttatt atattataat gttttctact aacgatatat ccagattaga gtcagatata
    12001 gtcttcatag ctagttcaaa atcaccaact ctttctggac aatgtgcatc tgagcctatt
    12061 ataaatttat tttctagccc ttttatttta tttaattgat ttacattcaa aaaagaatgg
    12121 ctactattta tctcaagtct agtatttgtc ttttttgctg tctttgctac ttcttctata
    12181 tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt acttatcgca
    12241 tttattacag cttttgtatt atattctttt gctttttcag ttttaaaaat atagttatta
    12301 caatgattta ataagcttct taatgatgtt ggagtagaac caaaatgata tccagccata
    12361 atataatcaa aatctctaat aaatttatca tccatatcta tatttccttt atcatctaat
    12421 atgttacact ccatacctaa aagtatattt atatcagtgt acttatcatt taaataatct
    12481 acttcttctc tcattttgag aatatctttt ttctttacac catatgcaat atgtttataa
    12541 ctatggtcag atattcctat tgtttttatt cctttagaaa tggctacctt tacattatcc
    12601 tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc cagtattttc
    12661 atttgttaat cctctccaag tattttgact tcttcttcca acataacccc aaacttggca
    12721 tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact tgcttctcct
    12781 tgattgacta caaatccaca atgcttctca gatacttgtg ctcctcttaa agtaagacct
    12841 ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct cttaaatgta
    12901 cttcctgcac tagcaaaatt taatggttgt ttagttattc ttctcgtcgc taaatcatcc
    12961 attaaacttt ttatttcatc ataatttcct tcttgtaatt ctaattctgc tgataaagct
    13021 atatatccgt ttttcgatat tatacttttt ctatacccaa attccatctg ttcatttgat
    13081 aattcaaata tattgccttc catatccatc agtcttactg acttcactat gtccttcatt
    13141 tcaccaccat atgctccagc attcatggct aatgctcctc ccaatgtgcc aggtattcct
    13201 gatgcaaact caaacccttt taattcttgc ttttggagag ctttccctaa aactgataat
    13261 aaagctcctg actgagcagt catcctagta tcatttatct cataagaatt gaaattatca
    13321 gctagttcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt tgaaccattt
    13381 ccaattatta aatacggaat agattcttct tttaccaact taagtacatt ttttaattgt
    13441 tcttcagttc taggtctaac taatatatct gctggtcctc ctaccctaaa tgaaatatgt
    13501 tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa taaatttata
    13561 tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt agctgtcatt
    13621 attatttaaa ttacattagt atatagtatt aactatattc aataatatat gattttgctt
    13681 atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat aaatatataa
    13741 taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta aaaaaagcat
    13801 caatatcact gatatcaatg ctttaaaagg caatttaatt cgtattattt atctaactat
    13861 ttttgacata gcaaagataa taaatccctc caactactat ggaacctcca acgatatttc
    13921 caagtgttac aaataccaaa ttagttataa attgaaccat agttatattt gctcctaatg
    13981 ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatcca cataatacaa
    14041 atagcattat tactgtccaa catgaagcca attttgacat tatatcttga gatgctgtag
    14101 ccatccatac agctaaaacg actagaatat tacaaagtat tgcttttaaa aacattgatg
    14161 ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct gcatctcctg
    14221 tcaatgtaga agaataaaac acaagtgcaa ctagcaaaat tgaacctact aaatttgcaa
    14281 aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg tccataagtg
    14341 caatagccat taaggtgttt ccagtgaata attctgctcc tgccactact acaagcataa
    14401 gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct atcttgccaa
    14461 aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct cctccaaata
    14521 aacctaatag aattgcgttt tgtgtcgaaa tatttgcttt tttaatgcct gcacttatag
    14581 ttgcttgtgc agtttcccca ggtgtcaaga atcctttact cataaatcct tcccccttaa
    14641 aaattatact ttcccttgtc tttttatggc aataattata taataacaat atgatatata
    14701 ataaatttta ttaacataaa
//
LOCUS       DAELNY010000090        14655 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.090, whole genome shotgun sequence.
ACCESSION   DAELNY010000090 DAELNY010000000
VERSION     DAELNY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.090"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            69..1235
                     /locus_tag="KPE55_002258"
     CDS             69..1235
                     /locus_tag="KPE55_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0493321.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            1352..2134
                     /locus_tag="KPE55_002259"
     CDS             1352..2134
                     /locus_tag="KPE55_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG0493322.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(2253..2906)
                     /locus_tag="KPE55_002260"
     CDS             complement(2253..2906)
                     /locus_tag="KPE55_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493323.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(2930..4294)
                     /locus_tag="KPE55_002261"
     CDS             complement(2930..4294)
                     /locus_tag="KPE55_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0493324.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            4952..6382
                     /locus_tag="KPE55_002262"
     CDS             4952..6382
                     /locus_tag="KPE55_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0493325.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            6485..7588
                     /locus_tag="KPE55_002263"
     CDS             6485..7588
                     /locus_tag="KPE55_002263"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0493326.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            7603..7839
                     /locus_tag="KPE55_002264"
     CDS             7603..7839
                     /locus_tag="KPE55_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0493327.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            7856..8905
                     /gene="vorB"
                     /locus_tag="KPE55_002265"
     CDS             7856..8905
                     /gene="vorB"
                     /locus_tag="KPE55_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0493328.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            8907..9647
                     /locus_tag="KPE55_002266"
     CDS             8907..9647
                     /locus_tag="KPE55_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0493329.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            9650..10189
                     /locus_tag="KPE55_002267"
     CDS             9650..10189
                     /locus_tag="KPE55_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0493330.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            10313..12412
                     /locus_tag="KPE55_002268"
     CDS             10313..12412
                     /locus_tag="KPE55_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG0493331.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLYTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPLLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(12679..13194)
                     /locus_tag="KPE55_002269"
     CDS             complement(12679..13194)
                     /locus_tag="KPE55_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG0493332.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(13286..14497)
                     /locus_tag="KPE55_002270"
     CDS             complement(13286..14497)
                     /locus_tag="KPE55_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0493333.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNISNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         5505 a         1835 c         2514 g         4801 t
ORIGIN      
        1 tggtatattt gttataaaag tggtgatttt gtgataaaat ttaattatat agaaataagg
       61 aggaaaaaat gaaaattaat tggaatacaa aatacacaac aatagcaatt tatacattta
      121 taatagctgc tagtagtatt atattttatc tagtatcatc acaaattgat gtattttcta
      181 ataatttaga tgctatattt acaacattac aaccttttat aataggattt gctattgcat
      241 atcttcttaa ttttatttta aagttttatg aagacagaat ttttataaaa agtgagaaat
      301 taaaaaaact aaaacaaagt tcaaaaagag gcttgggtct tttattaaca tatgcaacag
      361 ctacattgat actttactta tttatgcatt tcgtattgcc acaagtaatt gagagtattg
      421 ttggactagc aaatgatata cctatgtatg taaacaatgc aactaagctt attgataaat
      481 taatgactga tttaaatctt gatgagcaat actttaattt agcagttgat aagtggaatg
      541 agtttgttac atatattata aagtttgtaa cagatttaat cccaatatta ggaagtatgt
      601 taaagaatgt agcctctagt atatggaata ttgtactagg attaattgtt tcagtatact
      661 tattaattga taaagaaaaa ttctatggat taagcaaaaa aataacttat gcagtattta
      721 cagaaaaaca agctgctaga attttagaat taactcatag aagtaactat acatttggaa
      781 gatttttagg tggcaaaata ttagattcat tcattatagg tattctaaca tttgtaatac
      841 ttacattagt taaaatgcca tatacattat taatatcagt tataattggt attacaaata
      901 ttatcccttt ctttggacct ttatttggag ctataccatc cacgctaata gtgatgtttg
      961 tatctcctat aaaagctttc tggttattgt taattatatt aataatacaa cagatagatg
     1021 gaaacattat aggacctaaa atacttggag attcaattgg tatatctgca ttttggattt
     1081 tgttctcctt attggttgca ggaaaattat taggttttat tggaatggtt ataggagttc
     1141 caatgtttgc agtaatttat tctataatta aggatattgt tgaaagtaaa ttggacaaaa
     1201 agggattacc gacagatact tccgattata tgtagctaaa aaatattatg tataattaaa
     1261 aatatataaa ttaagcaata tttaaagcac tgagaaagtt aaactcttgg tgctttttag
     1321 atataaaatt attatagaag aggtgttttt tgtggcattt atagaatgta aatttaaatc
     1381 taaatatact ggaggagaaa ctgatataat tgtaattttg cctgtagcaa atggaaaaga
     1441 actttttagt ggtaaagaga tatatgattt tgaaggtaaa tttaaaacat tatacttatt
     1501 tcatggttta gaagatgacc aaagctcatg gattagatat actaatgtag agcattatgc
     1561 taaaaataaa aatatagccc tagtaatacc aagagtagaa actagttttt ataccgatat
     1621 ggcgtatgga caccaatatt ttacttttgt aagcgaggaa ttacctaaat ttgttagagc
     1681 tatatttcct ttatcagata aaagggaaga taactttgta gctggtatgt ctatgggggg
     1741 atatggagct ttaaaattag cgctttcaaa accaaaagag ttttcggcag ttgcaagttt
     1801 ttctggttcg cctgatatga ttcatgagtt agaaaatcaa caaatagaaa ccaatgcaga
     1861 cattctattt cacgattttg gtagaccaga agacgctaga aatagtgaga atgatttact
     1921 ttacatactt aagaacctta aagaaagaaa tgaagatatt cctaaaattt atcaattttg
     1981 tggtgataga gattttttat ataaaaataa tcagatgttt aaaaaatatg cagattctct
     2041 gggtgtagat gttgaatatg aagagtgtgt aggggggcat gaatggcgtc tttggaattt
     2101 atggataaag aaatttatag atataattgg atagtacact tatggtatta acaggtatac
     2161 tataaaatat ctttaaatat gataaagact gaaatcctaa aatcaaatta tgattttaaa
     2221 atttcagtct ctaagaattt aataaaattt tattaagttc ttacacgagt tattattgga
     2281 gtaaaattgt caactatatc tatatagtat ttactcaatt cctctaaaat ctcttcgcat
     2341 aaatccgtac tatgaccaag attaataaga aactgttttg taaaatgctt tattacagct
     2401 tgagaagata tatcgtactt gtgtgaagta taaactctaa ttatttcagg tttcatagca
     2461 agtataccaa tacaagaaag tattaattca tcatgttcta tagattcttt atcattaaca
     2521 tatcttttca tgaccatttc ggttacactt ctttgatagc ttaaaatagc ttcttcataa
     2581 gctatatcca aataaaaatt tctatatgct tcgtttttta gaaggaaaca aaggtcagca
     2641 ttcatagcaa gaaagaacat catcatagta ccttcttcat ggtagttata gattaaagac
     2701 tgcatttcat ttcttatatc ttcatatatt atagctccta tttcgctttt acttgagaag
     2761 taatagctta ttagacctaa atttatatta gctctttctg caatttgcct agttgttgtc
     2821 ttatgataac cttgctcaaa aaataattct ctagcagcac ttataatact agcttttgtt
     2881 tctttgccct tttctgattt tgacatattt actgtgtata cctctctttt caatcttttt
     2941 ttgaaaattt gctaactata aatattgtaa tagaaccacc tattatagga actaaaattc
     3001 tgtcaaatac agtaagaata ccaatcatat ttaataatgc caatataaca cttactataa
     3061 cagcatagat agctcctttt tttgtagcac ctttccagca aagaccaatt acaaatggtg
     3121 caaatattac acctgtacat aatggagctg gccaaaacaa taattctaat aaactatcaa
     3181 atttaagtgc tatgattgca cctattatag ctgtcaaagc tgatgcaatt tgagaatact
     3241 ttagagtatt ttttaaatca ttaaattcta tattaggatg taataatttg ttataaatat
     3301 ctcttgatat aatagcactc attgaggtca ttaatgaatc ggcagtagac attatggcag
     3361 ccaatatagc agctaagaat aatacagata ttaaaggtgg caaagtagtc gatatcattt
     3421 gaggtaaaac ctgaacagat gatatatttg gaaataaact accaccaata gctccaatta
     3481 ttcctatagg gaaagcaaga aataatagta taaatcctgc aattacacta cttttaactg
     3541 catttttagc attatcagca cattgatatc ttaaaaaact ttcataagaa aatattgagc
     3601 ctaaaactaa tggtaaaact gtatacataa aatcactaat cgatttattt cctggaaaga
     3661 atggttcttt taccattgat tgtaacataa gtaaagggtt gtcgccaact aattttaagc
     3721 ttatataagc tactattggc aaaaacacta tacaaatagc aagttgaata aatgctgata
     3781 ctgcaactcc ccacattcct gatgtatagg tatatataat tataactata actcctataa
     3841 ttacagctat atcataagga ataccaaatg cagtagatac agaaccaaaa gaaattaatt
     3901 gactacccat aatagctagt atggatattg gtgttacaag actttgataa gctcttaaaa
     3961 atacactatg accatatcta gtttcaatat aatcagcagg tgttatatca caagataagc
     4021 ttcttaattt tcctgcgaaa aatgcattta taaagcaagc taaagctgca cctatagtag
     4081 aatacattcc aacccaaagt cctctttcag ctccccattg aactccacca actaagcacc
     4141 ctcctccaaa agatgtagca gcaatggttg cagctagtat aggcaaaggc agatttctac
     4201 ctgcgattag gaaatcggat gctgttttaa ctttttttga aaaataaaaa ccaatccata
     4261 tgtatattgc acacgtaaca attaaaagta acatagtctt ttctccttta taaaatattt
     4321 taataaaata tacaaaatat atttaaaata tattttatat aaaagtagta cgatatttgt
     4381 tttattggtt ttagattaat atagctataa taatttatga tattagatgg aaattacgta
     4441 aaaatagaaa attgaaaatt ctgaaatttt tgcatattga tatttatctc atagattctt
     4501 ttttgtaatt gtaacacgct tttattttta aatcaatagt acatgtataa aaataaaagg
     4561 aatttaaata agaaaaagat atttttaata tctggatgta gtatagaaat atcttgaata
     4621 aattattctt gaataaaatt ataaaaaatt aaaatcacta gaaatatgat aaaataattt
     4681 aaaatacgca tttcaatgac atttcaaaac aagaaaataa taaaaaacaa acctaaatta
     4741 agtctaaata gctttttagt taattttagt agtaaaaaat aaaacttaaa aagtatttga
     4801 caattatgaa ttttgagaat ataattaaat taaaattata cacatataaa aatatattcc
     4861 tgaatcaaga atgagatatt taagattcaa aatatatatt attatacgtg aataataaaa
     4921 agtaagaaaa ttttataagg gaggggtaga tgtgagtagt caagaaagca aaccaaaagg
     4981 aaatcttata tcaaatttta gagatgatat gagagaccct aagaaaagag caatatacac
     5041 atattcaagt tctataaact tagcgtacaa ttttataggt tactttgtat caactgttac
     5101 aaatattttt ttaactgatg tggcaaaact tggtgtaatt atggcaggtg gtatgcaaat
     5161 tatacaatct ttcataaaaa ttgctgtagc tcctataaca ggtacagtat ttgacaaaca
     5221 accattcaaa aaaggaaagt attatccatg gcttagatat gcaccagcag cgttagcatt
     5281 aacatatgga atatttttca tagttccatt aatgaaaata agtccagcat tacttttacc
     5341 tttgatgaca gctttattat taatagcatc ttgtgctcag caagtagttt tcactataat
     5401 ttatgctatt taccctgtag ttgctaagac acctagagac cgagttatag gctcaactac
     5461 tacgaatata tttaaagaag ttggtaaatt cttagtaggt ttttcatatc cgttattatt
     5521 agttttattt gcagatgtat taggtggaga aggaatgggt tatttaggta cctacttatt
     5581 ctttgcagca ggatgccttt tagtattttg gttttcttca acagagataa taaaatctgg
     5641 agcagaagaa gaagttcttc aaaaagaaga gaagccaaag attaaagcaa gtgaaatggc
     5701 aaaagcttta ttcactaatg taccacttat gatggcattt tgtttagaat ttcttatatg
     5761 tataagatct ataggattag gacctttggc tccatattat tttaaatatg taatagaaga
     5821 tgaacgtgga ttagcagtat ttttatcaat catgcctttg gtaagtgtag cattcatgtt
     5881 tttcgcacca gtgtttataa aaatctgtag agagcaaaaa ctagcttcaa taatttcttt
     5941 ttctatatgt gcactatgcc acttattggt tgcatggact ccttggggga aaactacttt
     6001 aggagtaact atgttgctgg caattggtgg aggtttttca aatgtagtat caataataaa
     6061 tcttaatttc tttgccggtt cttgtgatta tgggcattgg aaatcaggaa aagatttgcc
     6121 tggtttaagt atgtcactat atccagttgc aatacaagta ggagtattac ttgcaactac
     6181 tataagaaca gttttaatga actcaatggg atatcaagct gatatggtag tcacagaagc
     6241 agttaaatca ggttttataa atatgatttc ttattctatg gcaatacctt taataatagc
     6301 tgttgttata gcaatactat atccagtaag tgacaagaaa ttaaaccaaa taagagaaga
     6361 gttaaatcaa agaaatgctt agttaaaaga aagttaagta gatggttaat ttaagctatt
     6421 aaagtaagta gacgcaatag cgtttatata aatgaaaata catatacaga gggggctttt
     6481 aaaaatgata gaaaaattat tgagaaatac cgtaaaagaa ttacatcaat atgttccagg
     6541 tgaaccaata gaaaaagtta aggaaaaata tggtgtaaaa gaaataataa aattagcttc
     6601 aaatgaaaat cctctaggac catcaccaaa agcaatagaa gctatgattg aaatgctaaa
     6661 acaaggtcaa ctttatccag agccagaagc taacgaactt agaagaaaat tagctgaaaa
     6721 gctagattta aaaccagaaa attttattgt tgctaatggt gcggataacg ttatcactct
     6781 aataggtgag gcttttataa atagaggaga tgaagttata tattgtaacc caacatttcc
     6841 atcatataga actgcaacta taaaaaatga agggatacca gtagaagtac ctttaacaga
     6901 agattataag tatgatttac aaggtatatt agataagatt acagataaaa caaaattaat
     6961 atgtgtatgt aaccctaata atccaacagg aacaattgtg gatgataaag agttagaaga
     7021 atttttaaag aaagtccctg aaaatattat aactatattg gatgaagcat atatagaatt
     7081 tttaacagtt cctaattatg tggatggact taagtatgta agggaaaatt ataacgttat
     7141 agtaacaaga actttctcta aaatatatgg tcttgcaggg cttagagtag gatatggtat
     7201 tgctaaagat gagataataa gaactttatt tacagtaaaa gagcctttct ctgcaaatcg
     7261 tgttgctata agtggagcaa cagcagcact tgatgatgaa gagtttataa aagaaacata
     7321 tgaattaaat agagttggaa tggcttactt taaagaagag tttacaaaaa tgggatttga
     7381 tgttgtagat tcacaatcaa atttccttta tgtagatatg aaaacagata tacctaagtt
     7441 atttgaagac ttgaaaaaaa gagggtttgt aataagacca agtgctacac atgcacgtgt
     7501 aagtattgga actatggaag aaaataggaa atttgtagca gttttaaagg aaatattaaa
     7561 tattaaagga gagccaagta ttggataata gatagaaata ttatgaaacg gggtgtagat
     7621 atgaacttaa aaataaacgc agagagatgt aaaagttgtg aatactgcgt tatatcttgt
     7681 aaaaaaggag cattaaaaat atcaagtaag ataaataaag aaggttatgc tcatgtagaa
     7741 gttgatgaga gtaaatgcat actttgtgga atttgctatc aggtgtgtcc agataatgtt
     7801 tttgaaataa ttaaagaaga agcatgtcaa gaggcttaga aggagttggg ataatatgaa
     7861 aaagatgtgg aaggggaacc acgccattgc agaagcagcc ctaagaggag gttgtgaatt
     7921 ttatgcagga tatccaatta caccacaaac agaggtaatg gaatttttat cacatagaat
     7981 gtctgaactt ggaagaactt ttatacagtc cgaaaatgag atggctgcta tatatatggt
     8041 atatggagca tatgcaagtg gaatgaggtc tatgacgagt tcttcaggac caggaatatc
     8101 tttaaaacaa gaagggattt cttacttatg tgcaaatcat tacccttgtg taatcgtaaa
     8161 tgtacagaga tgggggcctg gtcttggctc attggactca gcacagacag actatctaag
     8221 agatactaga gggggaggaa atggagatta tcatttgata gtttatgcac caaacagtat
     8281 tcaagagact gtagatttga tgtataactc atttgatgta gcggagaagt acagagtgcc
     8341 tgtcgaaatt ttgacagaag cagctttagg tcaaatgatg gaaccagtag aatttccaga
     8401 gtttaagaag agagaaggag atttaggttg gacttatgat ggaagtaata gagaccatgc
     8461 aaaggttgca gataaccaaa agccaacttt ttgtatggag aaacgtatga gaataactga
     8521 aaatgaacaa caatgggaag attatcaagt agaagatgca gaatatgtgt ttgtagcgtt
     8581 tggtatacca tcaagaacaa ctatgaatgc tgttagaaga cttagagaac aaggtgaaaa
     8641 agttggaata ataagaccaa taacagtatg gcctttccca tataaagcat ttgaaaaggt
     8701 aagtaaagat gtaaaaggat ttatctcagt agaatcaaca gacacaggtc aattagtaga
     8761 agatgttgct ttagcctcaa agaaagtatg taaagaaaat gtacctgtgt atggtttatt
     8821 tagtggaaat catataccaa aaacattgca agtaatggac acttatagca aaataaaatc
     8881 tggcgaaatc aaggaggtgt tctaatatgg ctacagaaaa attaacacct agcgttgttt
     8941 caataccaaa caagttttgc ccaggttgtg gtcatggaat agtaaatcgt ataatagcag
     9001 aggtaataga agaacatgga tatgaaaaaa atcatgtatt aacattagga gttggttgtg
     9061 tctgcaatat gaattttagc tggaatggtg ataagatgca aactgcacat ggtagagctt
     9121 catctacagc tataggagtt aaagtagcac ttccagatac attagtaatg acttaccaag
     9181 gtgatggaga tgcttatgtt ataggtcttt ctgaaacttt aaatacagca tatagaaatc
     9241 ataatgtgac tgtatttgta ataaacaata ataattttgc aatgactggt ggacaaatgt
     9301 catggaccac tatgccgggt caagtcacaa caacaagtgt aaatggaaga gatataaaga
     9361 ctactggtag tccactgaaa gttcctgaga tggtggcaaa cttctttgat gtagcatatg
     9421 tagcaagagg ttcagttcat agcccaaagg aaataattaa tctaaaaaaa tatataaaaa
     9481 atgcaataga agctcaatta aatggagagg gatattcctt agttgaaata ttagcacctt
     9541 gcccaactaa ctggggtgta tcactagaga aatctatcaa atggatggaa gaagaaatag
     9601 tgccatatta tgctcttggc gaatttaagc aaagggatgg tgaatagtta tggaaaaaga
     9661 gctagtattt gcagggtttg gtggtcaagg agttttaact cttggtctga ttgtagctga
     9721 gtcagcactt gaattgggga aacaagtaac ttggatgcct gcatatggtc caactatgag
     9781 aggtggaaaa gcctatagtg tagtaaagtt ttctgatgag agtataggtg ggcctgatat
     9841 ggaagaaata gatgttttag tagcaatgaa taaaccatct ttagattata taaatttagt
     9901 taaagaaggt ggaacagtag ttattaatac atctgcaata gatgagaatg taacattaag
     9961 agaagatata agtgtagtta aaataaattg tcaggaatta gctcaaaaag taaacaatcc
    10021 aaaagcagca aatatagtag tacttggagc attaattgct aaaactgggt tatttgaaaa
    10081 agaattagct ttaaaaacta tgtgtgattt ctttgaggaa aaaggaaaag gaaagtttaa
    10141 tgttcaaaat gaagcagcat tcatggaagg ctataataac gccatttgat aaattaccct
    10201 tatcttaagt gatttatctc aagatttaat aatctttagt gccaagagaa acttcatctt
    10261 gagataaaat tcacttaaaa tcacaactat aaacatatag gagcgtgaat ttatgagttt
    10321 ggaaaaactt attagaccaa aaagtattgc tatagtagga gtgactgata aacttggttt
    10381 tggaagaagt gcagcactta gtatagtaaa aagtaaggaa acagatagag tatattatgt
    10441 taatcccaaa agagaagaat tgtttgggag aaaatgttat aagacgatac aagaagttcc
    10501 agaagtagta gactgtgtag tagtatgtac gcctagaaat gtagttccat ctgtattaaa
    10561 agattctgga gagttaggaa caaaggcagc agtggtatat gctagtggat ttgcagagga
    10621 aggtacagag gaaggtacag atttagaaaa tcaacttata gaaatatcaa atacatacga
    10681 tatgaaaata ttgggtccaa actgcatggg gttgctaaat tgtatagata aggttaatct
    10741 atgggcagga ggttctaaat gggatttata tactaaaaaa cctggtatag gaattgtagg
    10801 tcaaagtgga tttataactg ctgaaattgt ttcaagtgat tattttaata tatcttatgg
    10861 tttttcaaca ggaaatggaa atatagttac actagaagat gcagtagatt tccttgtaga
    10921 tgatgattat gcatcagtaa tagcaattta ccttgaaggt ttaaaaaatc ctcaaaaatt
    10981 tatagatgct ttaaaaaggg cagcacaaaa aagaaagcca gtaataatat tgaaatcagg
    11041 aaggtcagaa aaaggtgcta tttctgctgc ttctcataca ggaaatttag ctggttcgag
    11101 taaagctttt gaaagtattt ttgaaaaata tggagtaatt tctgtggaaa atttagaaca
    11161 gtttatgtgt ttagcacaag cttttagtgt tttagatggt aatttacctg caaattctaa
    11221 ttttgcagca ataaacttct caggaggaga aaatactatt tgtgcagatt tagcagaaga
    11281 aaatggtgta gaacttgcag aaatatcttc tgaaactaaa gaagaaatga aaaaatatct
    11341 tccaggattt gcaacaccta aaaatccact agatgctaca acagcattat tccatgaaaa
    11401 ggatatgata gtaggtttat tacatacctt tgaaaaggat gatagtgttg gatttactat
    11461 ggtaggtgca aatataagag atgaagaaaa tgaaatgcat gaaacccttt gtaaagctgt
    11521 aagtgaagct agagaacaag gattgaaaaa acctgtgttt gcagttccta cacttgaagg
    11581 aaatagatat ttagattata ggactagatt agaagataat caagtgccta taatgtcatc
    11641 agttggaact acatttactt gttttaataa aatggctaag tttatagatt atgattattc
    11701 aaaaagaact ttagaattta aagctgttaa gaaaagagag tcaaataatg tagtagcatt
    11761 atcagaatta gatagtaaga ttgagatgaa aaaatatgga gtaccagttc caggacaagg
    11821 taatgcaaaa agtattgatg aattagatga aatcttaaaa tatattaagt atcctgttgt
    11881 tttaaaaata aattcttctg aaatattgca caaatctgat gttggaggag ttaaaatagg
    11941 aattaagaat agagatgagg cagtagatgc atataatgag attttaacta atgtaaaaaa
    12001 agcaaaacca gatgctaata ttgatggtat attagttcaa gaaatggtag aaagtggtat
    12061 agaaattatt attggtataa ctaatgatga ccaatttgga ccactgttat tagtcggttt
    12121 aggaggagta tttgtagagg tatttaaaga tactacatta tatccattac ctataaatca
    12181 tgatgaagct attatgatgc ttaaaaaact aaaatcattt aagcttttaa atggctatag
    12241 aggaagtgag ccttgcgata ttgatgctct tgcagatatg atggtcaaat taggaaaata
    12301 tgcttatgaa aataaggatg aagtcaagga aatagactta aatccagtgt ttgtatatcc
    12361 taaaggaaaa ggcgtatgtg cagtagatgc cttaatagtt aaatataaat agtttataag
    12421 ttttggtatt caaccataat taaagcaccg actgaaaatc acattttgta agttagatag
    12481 tgtgattttc agtcacccca aatttaattt aaaaatattt taaaatgaaa gtatagtttt
    12541 aagtttcatt gtgaaatagc actgtataaa caataaaata ctacgtataa aacgaataat
    12601 aaaaggtgtt ttaatgataa gaatcaatct tgattttcat taaaacacct tctttattat
    12661 attaaaaatt tttaaacgtt atattgtcac aggtgggttt gttggagctg catatgtacg
    12721 attagcaaat tcttggtcaa caaagaatag accaccctct ccagcaagtt taacacgttt
    12781 tatattgtct tcacaatttg tctcttcttc tgcttgttca gatataaacc aaagtaagaa
    12841 ttgagaagtt ctaaagtctg attcagagtt tgctacagct gctaagttgt ttattaaagc
    12901 tgtaaccttt ttttcatgag ctaacgtacg ctcaagaata tccattgcag aagtaaagtc
    12961 tgaatctggc attgggatag catcaagctg aacctttcct cctacattat gtaagtattt
    13021 gtaaaaaaac atagcgtggt ctgtctcttc tttatattga acataaaacc aattagaaaa
    13081 tcctttaagt ccttctgctt caaggtaaga agacatagaa agataaagat acgcagaata
    13141 taattcatgg ttaatctgct cattaagtaa tttttccatt ttagctgaaa tcatttttat
    13201 acctccaaat tttaaatgct cttataaaat ataataatat taaatggaaa aatattcaat
    13261 aaaagtgttt aaatatttta catatttaat aaaaatttat actaccatta aaaaaattat
    13321 aacttacaat ggctatagag ccaattaatg gtgcatttgc tccaaaattt gaatgcctta
    13381 cacttatttt tttatattta gaaaaacttg ctgaagcagt caattttttt aataatgtat
    13441 tttctataaa tgtaccaggt atattaaagt catagcctat tattatagaa ctacaatcaa
    13501 tcaatgtaag tgtattaact aatgcatatg atacgtatgt acaaaattca tcaagcaaaa
    13561 acatggcaag actatcttta ttatttgcct catctattaa cttgactaaa gaaaaatctt
    13621 tacagttaat taatggggag ttaggataaa tatggctcaa tgattcaatt cttttaatta
    13681 gattactgac acttgtataa taatctaaac atccattgtt accacaactg cacaatggtc
    13741 cactaaaatt aattgatgta tgtcctattt ctccactttg tcctaggttg ccagtatgaa
    13801 gtttattttc tagaactaga ccagctccaa taccattcat tatgtgtagg tatatataat
    13861 ttgatatatt tttacctaaa ccatacattt tttctgctaa tgcacccgaa ttagcatcat
    13921 ttatcaaaaa tattggcaaa tcagacattt cttttataaa atctactata tttaggtttg
    13981 atatatttcc aaaatctggg ggatttagta tggtaccatt tatatcatct actggaccta
    14041 ttgaagaaat accaattcca attatatctc tcttgttgat tttcattatt ttagtaaaca
    14101 ttctttttat tatatctttt aaaacatcat ttgatagaca tttagggtaa gtttcagaaa
    14161 gttgttttac aatattacct tttaaatctg aaataattac cttacatata tttcttctta
    14221 ttaggattcc acaaatacaa ggcgatatat tagaaatatc taataaaatg ggtttacgac
    14281 ctgatgaatg tgcatttaaa gttgatgact ctatttccct aatcatattt tcattaataa
    14341 gttcagtaac tagatttgat aaagtcattt tagtaagtcc tgtagactta gctaaatcaa
    14401 ctctggacat aggtgtattt gtagaaagta atttcaatat tattaactta tttttaattt
    14461 ttacatcttt gatgttagaa ccttgtttgt tactcatcat aaatctcctt aaataaagtt
    14521 aagtgtttta tttattttca agtatactat caccttataa tagagtaaca tttttttact
    14581 tgaaaatatc attaattaga atatatcaca ttatatatca taaaacatat aaaaaaatat
    14641 tagaattaaa ttaaa
//
LOCUS       DAELNY010000091        14607 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.091, whole genome shotgun sequence.
ACCESSION   DAELNY010000091 DAELNY010000000
VERSION     DAELNY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.091"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            404..1312
                     /gene="trxB"
                     /locus_tag="KPE55_002271"
     CDS             404..1312
                     /gene="trxB"
                     /locus_tag="KPE55_002271"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0493334.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1402..3225)
                     /gene="typA"
                     /locus_tag="KPE55_002272"
     CDS             complement(1402..3225)
                     /gene="typA"
                     /locus_tag="KPE55_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG0493335.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3437..3679
                     /locus_tag="KPE55_002273"
     CDS             3437..3679
                     /locus_tag="KPE55_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493336.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3827..4018
                     /locus_tag="KPE55_002274"
     CDS             3827..4018
                     /locus_tag="KPE55_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG0493337.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4302..6803
                     /locus_tag="KPE55_002275"
     CDS             4302..6803
                     /locus_tag="KPE55_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG0493338.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            6963..7631
                     /locus_tag="KPE55_002276"
     CDS             6963..7631
                     /locus_tag="KPE55_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493339.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7615..8892
                     /locus_tag="KPE55_002277"
     CDS             7615..8892
                     /locus_tag="KPE55_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0493340.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9293..9514
                     /locus_tag="KPE55_002278"
     CDS             9293..9514
                     /locus_tag="KPE55_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493341.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(9803..10567)
                     /locus_tag="KPE55_002279"
     CDS             complement(9803..10567)
                     /locus_tag="KPE55_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0493342.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            10841..11515
                     /gene="fsa"
                     /locus_tag="KPE55_002280"
     CDS             10841..11515
                     /gene="fsa"
                     /locus_tag="KPE55_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0493343.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GIKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(11637..13031)
                     /locus_tag="KPE55_002281"
     CDS             complement(11637..13031)
                     /locus_tag="KPE55_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG0493344.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(13109..14455)
                     /locus_tag="KPE55_002282"
     CDS             complement(13109..14455)
                     /locus_tag="KPE55_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0493345.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         5778 a         1779 c         2273 g         4777 t
ORIGIN      
        1 attgttagtg tgaaattaca tatatattca aattaaattc ataaatattt cacataataa
       61 tagtatactg taaacaaaag gaggtattaa atatgggaat agtacttata acagcagcat
      121 tagttttggt tatgtatgga ctttcaattc tatcatatat atcagaaagc aatatattag
      181 aagacactgt aaatgataac caataaggtt ttgaattata taaataagtg agaactgtaa
      241 ataaaagtta atttttattt gcagtttttt tatatatatg taaaataaag ttaattaata
      301 aaaaataatg ttttgtttat agtatatatg gataataata gtaaagtata agaatgatga
      361 gaataatata taagaaataa taatataaag agaggtaaac gttatggtag atatcattgt
      421 aattggtgct ggaccagcag gtttaacttc agcaatttat gctatgagag cagggttaag
      481 cgttacagta tttgaaaaaa atatttatgg aggtcaagtt gcaagtacct ctgaggtaga
      541 aaattatcca gcagttcaaa agatttctgg ggtagaattt tctaataata tatacaatca
      601 agcagttgct caaggtgttg atattcagtt tgatgaggtt gaagagataa atttagaggg
      661 aaaagtaaag gttgtaaaaa cttcttcagg tgaacataaa gcaaaggctg ttattcttgc
      721 aaatggagtt gaaagacgta aattaggttg tgcaggggaa caagaattta caggtagagg
      781 agtatcttac tgtgcaacat gtgatggagc cttttttaaa gataaagaag ttgctatagt
      841 aggtggagga aatactgcac tagaagatgc tttattttta gctaataatt gtacaaaagt
      901 gtatttaatt cacagaagag atagttttag aggtgaagaa gtgttggaga aatctgtgaa
      961 agcaagagag aatattgaga tactttacag tcatggtgta gaaaaaatag aaggagaaaa
     1021 gactgtatcg aaaatagaag taaaaaactt aaagactgaa gaaaaaagaa ctatagatgt
     1081 tagtggtatt tttattgcta taggtcttaa acctaataat aaaatgtttg aaaatgttct
     1141 tgatttggat gaaggtggat atataatttc agatgagtct tgtacaacat cagtagaagg
     1201 tgtatatgta gcaggagata gtagaactaa gtttttacgt caaataataa cagcagcatc
     1261 agatggagct attgcagccg ttcaagcagc taattatata aatgttgaat aatataaatt
     1321 tcaaataaaa atagccatat aaactgaaat aatttttcag tactatatgg ctattttgtt
     1381 aattagaggt aaatgtacgt tttatttacc ttgtctagat ttgttatatc ttaatctatc
     1441 atgttcattt aagaatcttt ttctaagtct tatagaatca ggtgtaattt caactaattc
     1501 atcatcttct ataaactcta gtgcttcttc aagagtaaat attctaggag gagataattt
     1561 tatagcatca tctgaaccag aagcacgtac gttactcatt ttcttgtttt tacaaggatt
     1621 gactaccata tcatcttttc tggaattcat accaattatc ataccttcgt aaacttctac
     1681 accagggtct acaaacatta cagctctatc acttaaggca ttaagagaat accccatagt
     1741 aacaccaggt ccttgtgcta ttaaaacacc atttcctctt gaaggtattt ctcctttaaa
     1801 ttcttcaaat ttttcaaatg aacgaactaa agtaccttct ccacgagtag cgtttataaa
     1861 ttcacttcga tatcctaaaa gaccacgagt aggtgctaag aactctattt ttacatagtc
     1921 tccttctatt gacatagact ccatcatacc ttttctcatg tttaattcat ttataatagt
     1981 accagagtaa acttcaggac agtttacaac aactctttct attggttcca ttagttttcc
     2041 atcttcttta tgcattaaaa cttcaggttt agaaacacca acttcataac cttcacgtct
     2101 catattttca agtagtatag ataagtgaag ctctccacgt ccagaaactt tgtatccatc
     2161 agtagtatca agaggttcaa ctttaagacc aacatttact tcaagttcct tttcaagtct
     2221 atctttcaaa tgtctagttg taacgaattt tccacttttt ccaacgaatg gagagtcgtt
     2281 tactaagaag ttcatagaaa gagtaggctc ttctattttt atcatttcca taggtagtgg
     2341 actatctaaa tcgcatatag tttcacctat agatatatca ggaataccag ctataacaac
     2401 tatttctcca ctagtagctt catcaacttc aacttgtttt aaaccttcat acacagaaag
     2461 ttttgatact tttcctttag atacaacaga atcttctcta catatagcaa cttgtgtatt
     2521 gtttttaaga gtacctttgt aaattctacc tataccaagt ctacctacat aatcatcata
     2581 tgcaagagct gatatttgga actgtagagg ttcattatcg taattaggat aagcttcaac
     2641 gtgatttaca atagttttaa atagaggaga aaggtcttca ctatcatcat ccatttcaag
     2701 tttagcaata ccttgtttag caataccata tattatagga aattcgcatt gctcatctgt
     2761 tgcatttaag tctacaaata aatcgaaaac ttcattaact acttcttccg ctctctggtc
     2821 ttttttatca atcttgttta taaataatat tggttttaaa ccgaactcta aagatttttg
     2881 taaaacgaat ctagtttgag gcattgggcc ttcactagca tcaactaata gaataactgt
     2941 gtcaacagtt ttcataacac gttcaacttc agaagaaaag tcactgtgtc ctggagtatc
     3001 aactatgttt attttgtaat cttcatagtt tattgcacag ttttttgaat atatagttat
     3061 gcctctttct ttttcaaggt cgttactgtc cataacgcag tctttaacta cttcgttctt
     3121 tctaaaaaca ccgctttgag aaaggaaagc atcaaccaat gttgattttc cagcgtcaac
     3181 gtgggctata actgcgatat ttataatttt atgtttttgt gacatttttg ttgctccctt
     3241 cgattaaaat aacttattaa ttatagcaca atatattatt aggtacaagg aggaatatat
     3301 taaattgtat taatatagtt taaattgaat aaaactgtta gttttgcatc tgaaaattat
     3361 tttgtttaaa aaaatttttt agacaaataa taaccttaat agataaaaag attaataaaa
     3421 gagtaaagga tgatatatgt tggaagtaac caaaataata attaaatatt ttgcaagtga
     3481 aagtgatact tttgtaaaag aagaagtttg taattttgaa gaacttctta agtggtctca
     3541 taaatttatt gaaggtaaaa agatagaaga tgttttgtat ataaagtcta aagaaaacta
     3601 taaagtaaat gtgattaagt ctttagaaat taaggaattg gaaatagtga gaaaacaaca
     3661 gataccaccg tacatgtagt attaataata tataaaatta atatgtaata ctcgcaatag
     3721 gatataaatg gtatatatgg taataattta tattaaaaaa acttttataa agtttatatt
     3781 tgggtataaa agtattaatt agacattaat taggaggaac ttaaatatga taagagtatg
     3841 tccaaactgt tcaaatgtgg atgtaaataa gctaaaaact ttggttggag aagaaaatgt
     3901 aaagactggt tgtataggtc agtgtagagc atttaaaaaa gaagctgttg gaataataga
     3961 caatgagctt gaaataaagg aaacagaagc agagtttttt gaagcatgta aagaataaat
     4021 tttaaataaa atataataaa tagagcatct aaattgtata agcattaaac aatttagatg
     4081 ctctattttt atatatacat ttaattatga gtatactttt ctattaaata caaataaata
     4141 taactaatac atttatttat taaaaaaata ttgatttaat atacctatag ggggtataat
     4201 aaaaatatag atttctaaat aagtaattta tcaatattac atatcaaaat aaaacgttac
     4261 aaattagctg aaaatatatt gaaagaaagg aagattttat tatgagttca aataaagtgg
     4321 tagaaagtta taaaattaca ggtatgacat gtgcagcttg tgcaaaggca gtagagagag
     4381 taactaagaa gatggatgga gtatatgacc aaagtgtaaa tatagctact gaaaaactta
     4441 aaatagaata tgataattct aaggttaatt ttgatgatat aaagcaggtt gtagaaaaag
     4501 caggatatgg aattataaag gaagaaagta ataaaaaaat tgatatgaaa attgatggaa
     4561 tgacatgtgc agcttgtgca aaagctgtag agagagtggt aaaaaaattg gatggagtag
     4621 aaagtattag tgtgaatatt gcaacagata aagctaatat agactatgac ccatcaaaag
     4681 taaaattatc tcaaataaaa gcagctattg aaaaagctgg atataaacca atagaagagg
     4741 taaaaaataa ggttgatgtg gatgaagata aattgagaaa agaaagagaa atgaagtctt
     4801 tatttgttaa atttatagtt gcaatagtat ttgcagtacc tctgttttat atagcaatgg
     4861 gacctatgat aataaagcct atagggccat ggcctttgcc agaaattata aatcctatga
     4921 caaatacttt taattatgca ttaattcagt taattttagt aataccagta atgatagctg
     4981 gttataaatt ttatataaat gggtttaaat cacttttttc tttaagccct aatatggatt
     5041 ctttagttgc aataggaaca ttagccgcat ttttatatag tctatatacg acactacaaa
     5101 tagccaatgg acaaattcaa ggtatgcacc atcatcagtt gtattatgaa agtgcaggaa
     5161 taataatagc acttatctta cttggtaaat atttagaatc aaaatcaaaa ggaaaaacat
     5221 cagaagcaat taaaaagctt atggggctac aacctaaaac tgcaatagtg ttagttgatg
     5281 gtaaagaagt tgaaactcct atagaagaag tagagatagg agatatacta ttagtaaaac
     5341 caggaactaa aatacctgtg gatggagttg taatagaagg atatacatca gttgatgaat
     5401 caatgcttac aggtgaaagt ataccagtag aaaagaatgt tggaagtaag gtgacaggag
     5461 ctagtattaa taaaaatggt gttattaagt ttaaggctga aaaaattggt ggagatactg
     5521 ctcttgccca aataataaag ttagttgaag atgcacaagg tacaaaagca cctattgcta
     5581 aacttgccga tactgtatct ggatattttg taccaatagt aattgcaata gctgtagttg
     5641 catcattatt gtggttttta ataggaggaa aagatatagt atttgtactt actatattta
     5701 tatcagtact tgtaattgct tgtccttgtg cattaggatt agcaacacca actgctataa
     5761 tggttgggac tggaaagggc gcagaaaatg gaatcttgat aaaaggtgga gaggcattag
     5821 aatctgcaca taaagtaaat acagtgatat ttgataaaac aggtactata acagaaggaa
     5881 agccaaaagt aacagatata gtattaaata ataatgtaaa agaagaatat cttatcaaga
     5941 ttgcttcaag tgcagaaaaa ggttcagaac atcctcttgg agaagcaata gttaagtatg
     6001 gagaagagaa gaatataaaa tttgaaaaag ttgataattt taaagccata ccaggtgctg
     6061 gtatacaagt tactattaat gatgaaagta tattacttgg aaatagaaag cttatgaatg
     6121 acaataatat aaagcttggg gatttagaag aaaaatcaaa tatattggca agccaaggta
     6181 aaacacctat gtatatagct gtagatggaa atttatcagg tataatagca gttgcagatg
     6241 tagtcaaaga aagtagtaaa aaagcaatag aaatacttca tgatatggga ataaaagttg
     6301 caatggtaac tggagataat gcgaaaactg caaatgctat agcaaatcaa gtcggtatag
     6361 atatggtttt ggcagaggtt ttaccagaag ataagtcaaa agaagttgaa aaactacaaa
     6421 atcaaggaaa gtttgttgca atggtagggg atggaataaa tgatgcccca gctcttgcaa
     6481 aagcagatat agggatagca ataggaagtg gaacggatgt agccattgaa tctgcggata
     6541 tagtacttat gaagagtgat ttaattgatg ttccaacagc aattaaactt agtcatgaaa
     6601 ctataaaaaa tataaaacaa aacctatttt gggcatttgg atataataca attggtatac
     6661 ctgtagcagc aggaatatta tatgtatttg gaggaccttt attaaaccca atgattgcag
     6721 cagcagcaat gagcttaagt tcagtttcag tcgtatcaaa tgcacttaga cttaaaaact
     6781 tcaaggcata taagagagat tagattaata aattaaataa aatgtatcca aaagagctat
     6841 ctttaaatta aaaggattaa tttacaaatt aagatagctc ttttttcatg taaaattcac
     6901 aacaataaga taaaatataa gtaatatacg aaattttaaa taaaatagaa aggattgata
     6961 gaatgaattc gtcaatactt gtaattgaag atgattcaaa tatacaagag ttaatatcag
     7021 aatttttaag tgcagaaggt tatcaagttg atacagcaaa tgatgggttg gaaggtatac
     7081 aaaagtttaa gcaaggaagt tatgatttag taatattaga tataatgatg ccaaacttag
     7141 atggttatgg agtttgtaaa atgataagaa agtcatcaag cgttccaatt atatttttaa
     7201 ctgctttaaa tgatgaagga gaccaactta aagggtttga cttagaatgt gatgattata
     7261 taacaaaacc attttcattt aatcttctta taaaaagagt agaagcaatt ttaaggcgaa
     7321 gtaacaagac tataaatgac aaatttatag tttttgaaaa attaaaatta gatttaaaca
     7381 catatattgc agagatagat ggagaaccta tagaacttac actaaaagag tttaatatat
     7441 taaaagcttt gatagaaaag tatccacaag ttataactag ggaaggtctt ttggatagta
     7501 tatggggtta tgactattat ggagatacta ggattgttga tgcacatata aaaaatataa
     7561 gaaaaaaaat atcattacca tatattaaaa ctgtaaaagg aataggatat acacttgaga
     7621 aggatatttg ataagtggga aaagttaagt ataaagtata agttattttc aataacgaca
     7681 tcattattaa tagcattagc tttaataata tatctaattt tatatttttt attgccatca
     7741 tattatcatg agtataaaat tgagtctctt caagaaagtt tgaaatcttt agtagatagt
     7801 tcaattcatt tcgatacata tactttagaa gaaagacttt attatatggc taaagaccaa
     7861 aatttggcta ttttattaaa agataaccaa ggtaaaattg tatatggtaa gaatgaagta
     7921 gttattttaa gatatagtaa gtacatgatt aatagtcttg aggatgagta tagaacttct
     7981 atacctatat atactaaaga tgcaaaagat ggtccatata ctttagaact cgttatgcca
     8041 ttacaaccaa ttgacgaagc aaacgaagtt ataagaaaac taatgccata catcatatca
     8101 atagcaatat tgatagcgat tattggagca tatatatatt caattgtaat aacaaagcct
     8161 cttataaaca taatagagag tgagagagaa caggaatata gaaggaagga ttttgttgct
     8221 acaatatcac atgaactaaa aactccaata accataataa gtggtcaaat tgaaggtatg
     8281 atttatagtg taggtaagta taaggatagg gatacttatc tgaaaaaatc gtatgagtgt
     8341 acacaagaat taaaagattt agtaaatgaa atgatagagg tttcaaaatc agaaatactt
     8401 gaaaaagatt tgaaattggt atcaataaat ataagtgaat tattaaatag acttgtaaag
     8461 agacaagtat ttttaataga agaaaaacat atgaaaacta ttttaaaaat agaagaaaat
     8521 ttggaaatta aagcagacca agagagaata actaaggcaa tcaataatat aataaataat
     8581 gccataaaat attctcctga agaatcagag cttataatta gactctatga taaaaataaa
     8641 cgaataagta aaaagacttc taatcaaaga gtggttttag aaattgaaaa tactggtgta
     8701 actatcgaga aacgttattt agaagaaata tttaatccat tttatagaat agaaaaatct
     8761 cgttcgagaa aaactggagg aagtggactt ggactttata tagtcagcca aatttttaaa
     8821 agtcatgggt ttgattatag cataaaaaac aaagaaaact ctgtagtatt cacagtagaa
     8881 tttaaaaatt aattacttat tataaagaat atataacttt caataatagt tcattttaaa
     8941 atcacaaaaa attcataatg aaactgtatt atataaatat aaataagctt aaaaccataa
     9001 aatacaccaa ctataatgat gagatttcct tacatcctcc atataagtca tcattatagt
     9061 aacccaacaa ttaagttact atgaatttaa aaaataacac cgagcatata atctatgctt
     9121 agtgttattt ttattttgaa ataaaaaata tttaatttat tgaaatgtat agatttagat
     9181 agtttaaact atatttaaga cttaaaaatt ataattgata ttacaaataa atatatgtac
     9241 tttgggaaaa ataaatatag gaagtattat atttacagag gaggtagttt tgatggatag
     9301 attaaaaaga gatatgaaaa atatgaaaga agatttaaaa gaaggtgtta aaaagacagc
     9361 ttatgttatg aaaaatacag ctgaagattt gaaagatgat gcacaaggtg ctatggtaaa
     9421 cttagaaatg aaaaaagatg agatgatgga aagatatgaa caaaagaaat tttctaaaga
     9481 attagagaag caaatgaaga atgaaatgaa ataagctttt atttatgaat aacaaattat
     9541 tgtattcata taaaaagtag ccagtaatat taaatagttt atttaaggaa atagactttc
     9601 taaaaataac tgtattagat ttagaaagtc tatttcaaat tagttttata aatagacata
     9661 aggataaagt agatgttata gaactagatt ttaaaaaata gatactaagg ataaaataaa
     9721 tattatagaa atatgttttt aagaatattt atcaaataaa aatttatatt tataagaaat
     9781 cctataaaac atataattaa acttataaaa catttacatt aaattctgat aaaatatcta
     9841 ctatatcagg agtaggcgtt ttttctgtaa ttatagaact aatatcttct aaatgagcaa
     9901 acttgaaatt tccatcaaat ttaaactttt tatcttccat aactataaat acttttttac
     9961 ttgaatttat tatagccttc ttcgtaagac catcttcaat ttcaaaagtt gtaagtgaag
    10021 agttgaatac atctactcca caacttccca taaaagtctt atcaaactta tatttcgata
    10081 taaactctat agttggagta ccgacaaaac cgtctaaact gagatttaat aaacctccag
    10141 tggaaattat atttaaatta tttggttttg aagtaactac atttagtata tccagcatat
    10201 ttgtaacaag tgttactttt ttattacttt cagctaacaa ctttgcaagc aagatgttaa
    10261 ttgtagagat atctaaaaat atagtttctc tatcttcaat taaattaaat gctttttctg
    10321 ctattatttt ctttgttgga atattaattt cttttctgtc ttttgtatcc tgattctcta
    10381 gtgtttctct agctagtaca gctcctccat aaattcttct tattgaagaa atcttttcta
    10441 tattttttaa atcttttctt atacaatctt ctgtaacatt aaatttagaa ctgagctctt
    10501 tgacagtaac tctaccttct ttttttagga tatttaatat agcttggatt ctttcttctg
    10561 caaacatttt ctacctcttt tcgcaatata gtattatagt aaatattata agataaaata
    10621 caatatatca actattttaa catgaaatac aaaattaatt taatataaaa agtatgtttt
    10681 ttctaactat atatttagaa tttagtaaat aaaaaaatta taaaactaag aaggaaaagt
    10741 aaaacaataa taaaaaataa aaaacaataa gcaaaatgtt gaaaataata ccaaatcaat
    10801 atataataag aataaagaaa gattaaaggg ggatatttta atgcaactaa tcttagatac
    10861 aggtaatgtg gaggagatta aggagttatg tacttgttta ccaatagatg gtgtaactac
    10921 aaatccaagt attgtttcaa aagaaaaaaa gaattttaag caacttataa atgaaattgg
    10981 tgagattata ggtgaggata tgcctataca tgctcaagtg ttaagtacaa agtatgaaga
    11041 aatattagaa gaagcattat atataagtag tttgagaaaa aatatatatg taaaaatacc
    11101 tgtaacacaa gatggtttaa gagctataaa agaccttcac agaaaaggta taaaaataac
    11161 tgctactgca atattcactg ctcatcaagg ttttttagct gcaaaggctg gagcaaatta
    11221 tattgcacct tatgtaaata ggcttgataa tatatctggt gatggagtag ctatggtttc
    11281 agaacttata aaaataatag atacttataa aatggataca aaagtacttg cagccagttt
    11341 taaaaatgca caacaagtta tagaattaat gcaacatggg gtgcatagtg ttacagtacc
    11401 atatgatatt tgtaaatcta tgatgaatca tccattaact gattggagtg tagataagtt
    11461 tatagaggat tgggagaata cttttggaaa aggaagtaaa acaaataata tttagtatta
    11521 tggtaaaaga gtagttttgt ccaatttcac aagaacttat ataaaaagat gaaaatatga
    11581 attttctttt taataatttc gttattatct taagttaatt catattttca tcttttttag
    11641 atttctagtc ctataaattt ccaccaaggg aatgttatac atattattat aaagtaggtt
    11701 aaaggaatca aagttaaacc gtatttgaaa ataaccttgc tattatacag attttcacca
    11761 tgaccaacca ttattgtagc atgatgaaat ggtaatatgt actgtatgct tactataatg
    11821 tagacaaata gacataaagt aacactattt tcctttagta tatgtaaact tccaagtaac
    11881 ggtattacta cagatgatgt agtgacagca cttccaagac atatattcaa gattattgtg
    11941 agagttatca aaaacaatat tgaaataatt tctgaagagt ttggtataaa ctttatcaaa
    12001 taatttccca ttatatctac aactccagag tactttaaca ctcctccaat tgaaaatgct
    12061 gctataaaaa aaataaggac cttccaattt atttctttta aagtactaaa tccaactatt
    12121 tttcttagta ataaaagaag tacacaaatt gacattataa ttaatgtgtt tatgttatgt
    12181 atgaactgag ttaaaaacat aaaaaacatt attcctaggc ataaaaatac gtgtttttgc
    12241 tctttgttta gcaaattaaa ttcttttttt aaattagaat atgaaaaatg aagtttatta
    12301 ccctcaagat tttcattaga aataaatttt attgaatgca attctttttt aaagacaaag
    12361 ataaataaaa aataagttat aaaacaagta attatagttg gaacagacat ataaaaagcc
    12421 cattgacccc aatttatatt acaatttcca agttctagta caacataatt tagaagagtg
    12481 tctccattta caaaaaacat tgatgtaaag gtagatgctg taaatatact aaataaaagt
    12541 atagattttg aatcttcagt tatctgttgt tcttttaaaa actccttgta aaaagcagat
    12601 acaagaataa cccttggaaa tggttgtggg ataaaaaata ttaaaagtat acctaaaata
    12661 tacgataaaa gaatcatttt tttaggggta tttacagtat ttaaaattaa cttctcagaa
    12721 ataatttttg cgacacctgt ttttgtaact gcattagtaa ttatgtatgc aagaattatt
    12781 atgtaaaaat tttctgtaaa taaaaactgt aaaataacct ttattggtgc aatgttaaat
    12841 ataaacgcta gaattacaaa aaacaatgca acaaaagttt tttcaactga gtctacagcc
    12901 catatcaaga tagttaaaaa agataagcat atagaaaatt ttatgttgaa actgtaatta
    12961 agagagaagt agagaattat ggtagctata acagtcatat aaaattgttt tggcttaaga
    13021 ataataccca tttttatatt cacctccata atttattata tcataatgga ggtgaataca
    13081 atgaatgtga atacatttta taaaaagatt attcattgat tctatgattt acaatagctt
    13141 tagttttcca atgtcctttc ttatagtaga taactgtaac aataagacct aaagtccaac
    13201 caaaaggaat acccatccaa attccgtctg ttccaaaaaa tttagaaagc atataagaag
    13261 ctggaagtct tccaagccat aaagatacta ttgagcttat aagtgcaaac atggaatcac
    13321 cagcaccttt cattgcactt gaaagtacaa aagaagttcc aataaataag aagaatggtc
    13381 ctattataaa taagtaagaa gagccaattt taatgacatc tttatcagta ttgaatagag
    13441 caattagatt ttccctaaat agcaaaatta aagcaataac tgtaactgct aaaactaagg
    13501 tcatttttat agattctcga atgccttttt taactctttc attttctcca gcacctatgt
    13561 tttgagcaac aaaagtagaa acagcagaac caagattcat aataggtaag cttataaaat
    13621 tttctatcct aagaccagca ccaaaagctg ccatagcaga tgtaccataa ctattaacta
    13681 aaaattgaag agccatattt ccaattgaaa acaacatttg ttgaactcca cttggaagtc
    13741 ctaatctaag tccatcttta aatattttgt tatcatatac aattcccttg attttaaatt
    13801 taagcacttc atgtttttta tttaaataaa ttatacttat tataaatgat actgcctgag
    13861 ccattatagt ggcaagtgca acgccctcaa cacccattct tagcactaca ataaaagtta
    13921 agtctaaaac tatatttaga attgtggcta ttatcagaaa ataaagtgga gttactgaat
    13981 ctccaagtcc tctaagtatt gcacttactg aattatatcc agcagaaaat aatattccac
    14041 taaatattat agtaagatac aattttgatt gagcaaaaac ggattctggt gtattcataa
    14101 gcttaagcat tgggccacca aatactattc caagaatact tattaatata gaaccaataa
    14161 ataaaagtat atatgtagta tcaatagtag cttttacctt ttttaaatct cctgagccat
    14221 aaaattgtga cactaaaata gaaaatccaa ggctaacacc cataagcgct gccaccaata
    14281 aaaacattat agggttagct ccagagactg ccgccattgc ttcttttcca ataaatctac
    14341 caactattat actgtcagct gtattgtata gttgttgaaa taaactacca ataagcatag
    14401 gcatggcaaa aaagaatatg gactttccct catgacctgt agttaaatct ttcacattat
    14461 tcctccaaat aaaaaaatta ttaatttatt atagtataat cttaacataa actttatatc
    14521 caaactataa ataaattgta tcaaaatatc atatattttt ttaaaatgta aagaatataa
    14581 ttataatttg aatttaataa atcaata
//
LOCUS       DAELNY010000092        14386 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.092, whole genome shotgun sequence.
ACCESSION   DAELNY010000092 DAELNY010000000
VERSION     DAELNY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.092"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1621
                     /gene="aspD"
                     /locus_tag="KPE55_002283"
     CDS             <1..1621
                     /gene="aspD"
                     /locus_tag="KPE55_002283"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG0493346.1"
                     /translation="HTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAGRGN
                     PNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDMPGA
                     YLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQEMKY
                     DISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPNLPR
                     YDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDETSCN
                     NLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWRLGT
                     FALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNHTAG
                     LSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAAYYT
                     QFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIRISL
                     ANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1742..2737
                     /locus_tag="KPE55_002284"
     CDS             1742..2737
                     /locus_tag="KPE55_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG0493347.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2915..3298)
                     /locus_tag="KPE55_002285"
                     /pseudo
     CDS             complement(2915..3298)
                     /locus_tag="KPE55_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3525..3622
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3763..4321
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3763..3792
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4607..4765)
                     /locus_tag="KPE55_002286"
     CDS             complement(4607..4765)
                     /locus_tag="KPE55_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493348.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5059..6429)
                     /locus_tag="KPE55_002287"
     CDS             complement(5059..6429)
                     /locus_tag="KPE55_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG0493349.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6645..7762
                     /locus_tag="KPE55_002288"
                     /pseudo
     CDS             6645..7762
                     /locus_tag="KPE55_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(8125..8205)
                     /locus_tag="KPE55_002289"
                     /pseudo
     CDS             complement(8125..8205)
                     /locus_tag="KPE55_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8245..8913)
                     /locus_tag="KPE55_002290"
     CDS             complement(8245..8913)
                     /locus_tag="KPE55_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0493350.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9766..12186)
                     /locus_tag="KPE55_002291"
     CDS             complement(9766..12186)
                     /locus_tag="KPE55_002291"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG0493351.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12540..12890)
                     /gene="rsfS"
                     /locus_tag="KPE55_002292"
     CDS             complement(12540..12890)
                     /gene="rsfS"
                     /locus_tag="KPE55_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG0493352.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13007..13579)
                     /gene="yqeK"
                     /locus_tag="KPE55_002293"
     CDS             complement(13007..13579)
                     /gene="yqeK"
                     /locus_tag="KPE55_002293"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG0493353.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13580..14269)
                     /locus_tag="KPE55_002294"
     CDS             complement(13580..14269)
                     /locus_tag="KPE55_002294"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG0493354.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4843 a         2363 c         1828 g         5352 t
ORIGIN      
        1 tcatacttat aaagaaatca aaaacatata tggaaaaata agcccttttg aatttaagga
       61 caaattaata gatattgcaa aatacactgc aaaagaaaat aacagagaat tacttgatgc
      121 aggtcgtgga aatccaaact ggacttgttc aactgctaga gaagcctttt ttacttttgg
      181 tcatttcgct attactgaaa ctcgttctaa ttgggattta ggtcatttgg ctggaatgcc
      241 tcaaaaaaag ggtattaaag aaagattttt taaatttata aatgaaaata tagatatgcc
      301 tggagcatat ttagctagag atattataaa ctttggtata aatgaacttg gatttgatgg
      361 agatgaattt gttcatgaac tagctgatgg aattattggt gataactatc cacttccaga
      421 tagaatgtta cctcatatgg aaaaaatagt acacgattac ctagttcaag aaatgaaata
      481 tgatatatca ggtaaatatg gtgatgtaga aatttttgct gttgaaggtg ctacagctgc
      541 tatgtgctat atatttgatt cattaatggc aaatgaactt ctaaaaaaag gagataccat
      601 agccttaatg actccaatat ttacacctta tcttgaaata cctaaccttc ctcgttacga
      661 ctttaaagtg gtaaatataa atgctaatga agttgatgaa aaaggtgctc atacatggca
      721 atatacaaaa aaagaattag agaaacttcg tgacaaatct attaaagctt tattcgttgt
      781 taatcctaat aatcctgctt ctatagctat ggatgaaaca tcttgtaata atctaataga
      841 tgtaattgaa aattataata aagatttaat gataatatca gatgatgtat atggaacttt
      901 tgtagaagat ttttcatcct taatgtcaaa actaccttac aacactgttg gagtatattc
      961 ttattcaaaa tattttggag ttacaggatg gagacttggt acatttgccc ttcataagaa
     1021 aaatgttttt gataagaaaa taaatgattt aactggagaa ttaaaaaaat cagtagataa
     1081 gcgttacagt gatatgagtc ttaacccttc tagcctttca tttatggaaa gagttgttgc
     1141 tgatagcaga cttgttgctc tcaaccatac tgctggtctt tctactccac agcaagtaca
     1201 aatggcgttt ttctctgctt ttgcattgat tgacaaagta gatgcctaca aaaaactcaa
     1261 tatgagtatc tgccatagac gtcaaaaact attatttgaa gctttagagc ttccaattaa
     1321 tgaaaacaaa aacaatgctg catattacac tcaatttgat attgaagaat gggctaaatt
     1381 aaactacgga gaagacttat ttaaatttat aagtaaaact gcatctccag tagatgttct
     1441 ttataaatta gctaatgact attcagtagt tttacttagt ggaaatggat tctatggtcc
     1501 tgaatggtca attagaatat ctctagcaaa tctatatgat gaagcttata ctaagatagg
     1561 aaaagccata agagagatat taaatggtta tatcgttgaa tggcaaaaaa ttaagaaata
     1621 attatattac actgaaatat ctaaattata tgaataaaag agtttatgta gaccttaact
     1681 actttttttg atataatcct atcggaatta taaattttat ttatgaaagg cagagaatag
     1741 tatgagtatt aagaaaaaag ttgccatagt ttttactggt ggaactatat ctatgacagt
     1801 agatgataaa gtcggagcag ctataccaac tttatcaggt gaacaaatac tatcaatggt
     1861 taccaacatt gataaagttg cagatgttga agtttttaat tttgatgaaa ttccaggacc
     1921 tcacataaca ccttatagaa tgatggaatt gaaaaattat gtaaatgatt tattagcaag
     1981 aaaagatatt acaggtgttg taataacaca tggtacagat agtttagagg aaacagctta
     2041 ttttctagac cttacaattg acaacatcaa acctgtaata gtcacaggtg ctatgagaag
     2101 tagttctgaa cttggatatg atggttcaag taatttatct gcatcagttt gtactgcagt
     2161 atccaaagat gctatgggaa aaggagtcct tgtagtttta aacaatgaag tattattagc
     2221 ttctgaagct actaaaacaa atactctttc tctaaataca tttaaatctc taactagtgg
     2281 cccattagga ataattgact gcaacgaact tgtccttaca agagatattg taaatagaac
     2341 aattatagat actgataagg tagaatctaa agttgcctta tttaaagcat atgtaggaga
     2401 aaatgctgac tttataagat ttgctgttga tagtggttat aaaggtatag ttattgaggc
     2461 aatgggtaga ggtaatattc ctcctcaaat gctttctgga gttgaatatg ctagagaaaa
     2521 aggacttcct gtagttatag tttctagatg tcattcaggt agagtattcg atagttatgg
     2581 ttactttggt tctggtagag atttaaaaaa tcttggttgt atatttggtg gggacttacc
     2641 aggtcaaaaa gctagaataa aacttatctt agcacttgga aaaactgata accttgatga
     2701 aatcaaagat ttctttgaaa aaggaatcta ttgttaattc taataacttc tatttaatta
     2761 tttattgata tttcaacatt tttaaatcaa aatctaatac ttatgtcagt taaatgtcag
     2821 caaaatattt tatccacaag ttttccacaa cttatcccca atattatcca caagaaaaaa
     2881 ggtagcactt atattagtag ctacctttta tttattaatc ttcatctttc cagcttttta
     2941 tccaatttcc tatttctatt attttttctt cagttatttc ttctccatca tgcaattttg
     3001 aaattaattc tgataatgga ttgttatgta gacctccaaa aattcttttt ccctctattt
     3061 tgagatattc tttctctttt attaatactc tatagtgtac atgtttccca attctctgcg
     3121 atatgaaaaa acctttttta gttaatctag ctaaaagtgt aattgtagtt gtatgcttcc
     3181 aagcatatgt tttttctatt tcatctgcaa catcttttga tattacagtt ttataaccag
     3241 tattctatat gtatttcata atttttaatt ctgcatctgg taatttttta ttcaacataa
     3301 tatattcctt ctttcaattt atgtcacttc ctcaaactta tctagcttaa attaataata
     3361 ggagcttttt atcacaaaaa ctacaggtaa atttttctat atattctacg tatttagtct
     3421 gttttttaag tgtgaaatta cgttcatacc tataaattta ttcaattttc aaacaatctt
     3481 attgaataat aactaattct attgtattat ttagttatag aataaaacta aatcggcaaa
     3541 actagagaaa tctagtgacg caaagctata gagactaaga cttatataaa tatcttatga
     3601 gttatgtcag ccagttgcca aagagatatt gttctttttg tttttatgaa agttttttag
     3661 ggggataata atttatatat ttagagataa aatagataaa tgtacacata tattgactgc
     3721 ttatattagc agttcgtatg attattgtaa ttttttggat acaatttaca ttccatatag
     3781 ttaatataaa acaagtgttt cgaatttctt ttctgaaact ataccaacaa tttacattcc
     3841 atatagttaa tataaaacgt tatatactga aagcggtgaa actggttacg tagcaattta
     3901 cattccatat agttaatata aaacctgtgc gtgtaggaga tggtaacaca actgttgaag
     3961 aatttacatt ccatatagtt aatataaaac acacatctac atgtttttct atctgagttc
     4021 atctctaatt tacattccat atagttaata taaaactttt taatccttct tctgaactcc
     4081 caaagtttat atcatttaca ttccatatag ttaatataaa acccacgaaa tttaaggtcg
     4141 atatatcaga tttgaaaaaa tttacattcc atatagttaa tataaaacaa gctaaatgga
     4201 gcaaccgacc aagtcgctag aaataattta cattccatat agttaatata aaacttttct
     4261 attaagtgta agcagaatgc atggaatatg aaatttacat tccatatagt taatataaaa
     4321 ccccaaaata aattgagtat ttccaatgcc tacacatata caaatctctc aaatttgcag
     4381 tgaaccatga gtagtgcaat tgataacact tatcacaagt cctcaatgcc ttatatttca
     4441 actgttaagc cttaatttct tcaaaaaatc gaacactgca aaatttctat gactttatta
     4501 tatcataaaa atattatttt tgaatatctg taccaatttg tgatataata aaagcaagaa
     4561 gaactacaat ctattttgct gtagagtgga gttcataatt tagaaattac ttagttttat
     4621 ggaatctgat ttttaaatca aattcccaac cactcttagt tgccgctttg agtggttttt
     4681 tacgctttct aaatagtttg ctagctatat aaactattaa gctagcagat agacttgcta
     4741 gtatgccttg taaaaaatta tccatgtata ttcacctccc ttcttaacgt cgggaggata
     4801 attttttgat acatgaactc cactctataa attgtagatt acatcttctt gctaaaaata
     4861 ttataacata taattcttac atattttacc tatttattac tttttacatt ataataatat
     4921 tcttttaatc aaaaaaacac ctactattaa agtaaatgtt tttaagtatt tttaatttta
     4981 aaatccatat agttcatatg aaactaaata taataactca acatatgagc aatatcgaga
     5041 catttacata cccactagtt atttatattc aaaaaaatct tctccatccg ggttataaaa
     5101 taatattcta cttgccaatc cttgttcata tttataattt tctttatcac ctacataaga
     5161 aaatgttaat gtcatgcttc ttgatggatc taatgaaatt atatctaaat aaatagtaca
     5221 tttttctcct ggctttaata caccgaaact attaaaatcc atttttattt tagcgtattg
     5281 ccccatatat aatgttgaat tagcttctcc ataaactttt aattttgtta catctaaatt
     5341 ttgtgtactt gtattttcta tatttatagg aactgttggg tctttatatt tttcagtata
     5401 atattcaagg ttatcatcat aacattcaag ataacttcct ttatcttgcc aaattatgtg
     5461 atttgaatca aaatcaatct ttaatccaca tattatattt ttatttgaag atggaagact
     5521 aatattattg atggtatttt cacactctag cataatttta tcacttagat ttcctatccc
     5581 ggttactttt gcagcatttt taagtatatc tttattactt ccaatatcaa ctagtacaat
     5641 aggattattt ttagccattg gtgaagcaac taaagcataa actaaatcag taccactagc
     5701 tatataaaat tcttttgcgc cattatagaa cttatttact atttttttat tagtatcata
     5761 tctatctact ccacccaatc gagttgaatt agtatcattt actaaactat cactcattga
     5821 tgaagtgcca cctattgcat aactttctat accagttgta ttaaatggaa cactttttcc
     5881 atcagttaat actatagctg ctttatctct aactgctaca ggtgcagcac tcatagcatc
     5941 tggctcacct ttgaaagcgt tagttaatat tactttattt actttattaa tggaatttat
     6001 ttcttttgct acattataac ttgtcttaat cctatcatta ccttgaagcc ttctagtctc
     6061 tattccttta tcttttagaa aagtttctgt tgctttatca atggaacttt ctcctcctat
     6121 tatgtatacc tttttagctt tttctactct tttaagagtt gcattaggta tattattctt
     6181 tttagtcaaa aggattggtg catttgttgc tcctgcaagt ccactcgcac ttagcccatc
     6241 agccattgta ctatctgcat ttataagtat tgcagtagta taactttgtt tgtcagctat
     6301 gataccagca gtttcgtatt tatctgctcc ttgaatttta tcaattttat caagtgcatt
     6361 agctgatata ggaaaaccag caacaaacat tgacactaac cctagtgata ataatttctt
     6421 taatttcaca atatctcccc cttttatata tgaaatacta tatatcttaa taattctaca
     6481 ttaaattttc ttttcctcct ttaacgatat ttatcttcct gttaatttaa gtgtatacaa
     6541 atacgtgtta taatgtaact gtaaagaatt aaaaagataa tgacataatt agagtagtac
     6601 aactgttctt ttataaaact aacttataaa gggagcgtac atatatgaat atcaaatcag
     6661 cttttataag aaaaagaggg gaaaaattct atgtatatgt ggagtacata gaggaaacaa
     6721 ctggcaagat aaaacagaaa agttatggaa gttatgaaaa gaaaaaggat gctgaaaaac
     6781 atttaattga aataaaatct actataaaca ataataagtt tgttgctcca aaagatatga
     6841 catttgtaga aagatgttat aaatacatgg acatgaataa aaataattgg tctccatata
     6901 ctgttacaaa tacaaaatct tgggttaaaa attacataga acctcttttt aagaatctaa
     6961 aactcacaga agtaaatcct agttcagtac aagcttttgt aaattataac tttaataatt
     7021 ctacctttac tagtgctaaa gtaaggtata gctttctaag ttctgtaata agggaagcat
     7081 atagattaag agaaatacaa gaaaattcat gtgattttat aaaacttcct tctaaagaaa
     7141 aatattctac aaatgaaata tacaatagaa aagaagtatt attgctaata gaaaagttac
     7201 ttagtaataa tatagaaata cctattcttt taatgttact tctaggttta agaatgggag
     7261 aagctactgg gcttaggtgg tcagatgttg acttagataa caatctgata aatgttaatc
     7321 aaattcttgt ctattctaat ggcaaaatat cttttaaaga gccaaaaaca ccaaaatcaa
     7381 aaagagcttt atcagctcca aaagaattaa ttgaaaaatt gaaaattaaa aaactaagac
     7441 aaagcaaatt aaaattgtaa ggtatcctgg aaaacagaaa taatttagtt tgtctaaaca
     7501 ccaatttaaa tccgtttcta ccttcttcac taagaaaaac gtttcacaat tttacaaaaa
     7561 aaataattta aaaaagatta gaatacatga tttaagatat acaagtgcca ctctgctgct
     7621 tttaggggga actaatatga aagttgtttc tgaaagattg ggtcatacag atataaaaat
     7681 aactatgaat agatattctc atgtacttaa agaaatggac aaagaagctt ccgacaatct
     7741 aagcgaactt ctatttaaat agtaaaaact gactgtcagt taaatgtcag ttaaacctaa
     7801 aaaaataggt tttgtcagtt gattatcagt caattatttt ataatagttt cacttaaaaa
     7861 cgtctatatc tacacatttt actatttgtt tatgcttatt aatatctatc tgttattatt
     7921 aattctaata gcttctattt aattatttag ttcagtttac aggaaactac acacaaacca
     7981 taaaatagga tgttgacaat ataacagata gtatgctcct gtaaaaggta acgtactatc
     8041 tgttatgttg tctggatttc caaatggtta ccctttggca cacaactttg ctatctcctt
     8101 ggtaaactaa aagtttgtaa tctaaaatcc taatccatct ctataaaatt taattgactt
     8161 ctccatactt tttacaccta aacatataaa agtaatttta ttcacttaat gacctccata
     8221 attcccaaat tagctatttg gaacttaact aaaaaatcct aatataaggg caactattat
     8281 aatggcaatc ggaatacata ctgtgtaatc ctgttccata gagtcatcat ctgaatgtag
     8341 ttttacaatt cgcaatttag caatcagttt gaagcagaaa tagcctatta ttgttccaat
     8401 tacattcgtg agtaagtcgt ctatatcggt tgctctgtgt aacgtaaaaa gctgaatagt
     8461 ttcgataaaa agagataatc caaaaccaat caagaatgta tttctcaaac gttcaaaggt
     8521 tttgctgata agcggacata aaaaacctaa tgggacaaaa agtaaaatat tcaaaataaa
     8581 actcaaacta aatccatcac taaaaggaat aagatttatg tttggattaa agaacgtttc
     8641 ccctaactgt gacagtctta tatattcact gaatgtaggg ataccaacaa tatgcgatag
     8701 tgttaaacat aagtagtaat acattgccag tgataaaata gatgttttta cctttggaat
     8761 atcactattt ttcttgaata ttacaactaa gaaaatacca atcaagataa ttcccaacaa
     8821 gaaaatagga ttaattaaca ataaatcttt gatagcttca aacagactga gttgaatttc
     8881 tacacctgat tgattatttg ttacagtctc catttgtaca tacctcctca taacatttca
     8941 attatttcta aaacacaaaa tagaagataa atcttaaaat catcctatca aaatttttaa
     9001 atttgccttt atcagtttat accaccagtt aattcctgtc ttacttctgt atggtattct
     9061 tgggattctt tcatcaaatt agacaaaaaa tggttaccat atcaataaac tggttacgct
     9121 ccatatctcc cgaagcaacc atcccctgag aataaatccg tatcaggaat ctccacatct
     9181 actgcaccta tcacaagtta tttgataaca tcctcagaca atcccaactt ggatatatcg
     9241 acaacaccca ttagaatacc actgctgacc atacttgttt tcctgctgat aattttaatc
     9301 aactgggaag ttgaaacgct atctttttta caagttcctt tttagaaaca tggtagctat
     9361 tatatgaatc tgaatttcgt tgtctgggtg tttcatataa agcatagata cgcacctttt
     9421 ttgcctgttt tcattatata gaaaaaaatt tcaaaataca ataattatat ttttttcgtt
     9481 taagcttaga gcagttcctt gatgaacaac actttggaaa ctgaatgatt tacataccac
     9541 ttaatacatt aactcgattt aacattaatt ataataatat ttaaaacaac aactagggta
     9601 tataagaatg aactttttag ttctttaatc atttttttgt ataaaatcaa atctattttt
     9661 ataatttcaa caaatttata atttcaacaa tgaaaaaagg cttatctcta ttttgagaca
     9721 gcctcgttta ttttataaat ttatattttt gttcatattt ttgagttatt taacaactat
     9781 atttactagt tttttaggta cagctacaac tttaactata gttttacctt caactaatcc
     9841 ttttattttt tcatcttcta aagctacctt ttccatctca tctttagata tatttgagtt
     9901 aacagttaat tttcctctaa cttttccatt aacttgtaca acaacttcta tttcatcttt
     9961 tactaaagct ttttcgtcat atttaggcca gtctatgtca aatacacttc cttctttacc
    10021 tatcatagtc catagttctt cacctaaatg aggggcaaat ggtgcaatta tagttactat
    10081 agtttgtaca ccttctttta taactgcttc gtttatatta tctaactctt tatatttata
    10141 catatcattt attaattcca taagagctga tatagcagta ttaaatccga atttttcact
    10201 taaatcagca gtaacttttt taagtgtaga gtgtatagta tatctcatat ccttatcttg
    10261 ggaatttaat tctccaaact ctacatcctt tttaaccaca tctgctaact cgtcaaccaa
    10321 tctataaacc ctatttaaga atctaaagca tccatcaaca ccttgctctg accagtccaa
    10381 atctctttct ggtggtgctg caaataatac gaaaagtctt gcagtatctg ctccatactc
    10441 atctattata tccataggag atactgtatt ccctttagac ttactcattt tagaaccatc
    10501 cataagtacc attccttgag taagtaagtt tttaaatggc tcattgaagt ctaccattcc
    10561 catactctta aatgccttta caaaccatct tgcataaagt aaatgcatta tagcatgttc
    10621 tactccacct atatattggt ctactggatg ccatctatta actaattcct tactaaatgg
    10681 ttcattttca ttattgctat ccacatatct taagaaatac caagaagaat caacgaatgt
    10741 atccatagta tcaacttccc ttctagctgg ttttccacag tgaggacaag ttgttgacat
    10801 aaattgcttt gaagtcgtaa gtggagattc cccttttcca gtaaattcaa catctgttgg
    10861 taaaagaact ggtaaatctt cctttttaac tggaactatt ccacattcat cacaataaac
    10921 cacaggtata ggacaacccc agtatctttg tcttgaaact aaccagtctc taagtctata
    10981 attaattgtt ctctttcctc taccttcttt ttctaactta tcgattattc cttcaaatcc
    11041 ttctgaagca tctaatccat taaattcttc tgaatttatc attttgttat cttctgttat
    11101 tacatcaatt atgtctaaat tatatttttc agcaaaatct ctatctcttt catcatgtgc
    11161 tggaactgcc attatagcac ctgttccata ctcaactaat acatagttag ctatccatag
    11221 aggaactttc ttgccattta aaggatttat aacatatcta cctataaaca ttccttcttt
    11281 ttctacatca gctgctgttc tttctatttc tgtcatagta tgcatttttt gaacaaaagc
    11341 ttcaacatct gcttcatatt cagtccctgc aaccaattct ttaactaatt catgctctgg
    11401 agcaagtacc atataagtaa ctccataagt tgtgtctggt ctagttgtaa atactgtcat
    11461 agatttatca gttccatcta tatcaaaaac taaatctgct cctgtacttt tacctatcca
    11521 atttttttgc attgttttaa ctttttctgg ccatccatct aatatatcta agtcattaag
    11581 taattcttct gcaaattcag tagtcttaaa ataccattgt tctaaatctt tctttaatac
    11641 tgttgcttta catctctcac atgctccttg aacaacttgc tcatttgcaa gaacagtctc
    11701 acaagaagga caccagttaa catatgattt tttcttatat gcaagcccat gctctaaaaa
    11761 cttcaaaaat atttcttgtg taaatctata gtattctggc gttgaagttg caacttcttt
    11821 atcccaatca taactaatac ctaataaatt taattgctct ttcatttctt ctatattttc
    11881 cattgtccat ttatgtggat gtattccatg ttttatagct gcattttcag ctggtaatcc
    11941 aaaagaatcc catcccattg gatgaagtac attatatcct tccatttttt tgaatcttgc
    12001 gacaacatca cctatagaat aatttctaac atgtcccatg tgtatttttc cagatggata
    12061 agggaacatt tctaatgtgt aataattagg tttttcagtt tgtgcagtat ccattttata
    12121 ctgattatta tctttccaga ttttttgcca tttcgattct acttctttaa aattatatac
    12181 gctcatattt tgcctcctat ttttaattgt gtataactac cttatttagt attatttact
    12241 tataatttcc aaataattct ttgaaaataa aaaatcctcg tcactaaact tacgtttagg
    12301 gacgaggata aatcttcgcg gtaccaccct aattagccta ctacatatat agactctctt
    12361 agatagcttt taatccagcc atggagaata gattttaatc tagtaagttc aaagttatat
    12421 aatattagtt ctcaccttcc actaactctc tgaaaaaata taatctttta ctgctctagt
    12481 ctattcaatt taatataatt tttgaaattt tctaattata ataacatata ttatcatgtt
    12541 tatgctaatg tgtcaatatc tatataaggt gcatctttcc ataatttttc taaattataa
    12601 aaccctctat tttcctcatt aaatacatga accattatgt ctccataatc aaggagtacc
    12661 caagtttgag ttccttgtcc ttctttacct cttggctcta atcctagttt tgctaattca
    12721 tcttccacat tgtcagcaat agcttttacc tgtctctgag aagatgctgt agtaataata
    12781 aaataatcac acaaactaga aacttttcct atatttatta taactgtatc ttgacccagt
    12841 ttatcttcta tggcatcata tgctattttt gtcatttgtt ccactgtcat ataaaaattc
    12901 ctcactttcc taattaaaat acacttagtt attgtaacat attaatacga ttttgtaaca
    12961 tagtcaaatt taaatttatt aatatgtttg cctttatata gcttttttat aataactttt
    13021 ctttaattag atagtttctt gccataactg ttcttggatg tatctcttct tttttattga
    13081 tgacaaacat taaagtatta ttaaaagaag ttaaaagtgc ttcatcaagc ttccctccat
    13141 atgctaattc tcttaatgta tctacaactg ggaattttct tccttcttca attaaatcag
    13201 ctatgtaaat tattttttct aaaagagaca tgtcctctct ccctgttgta tgatatttga
    13261 ttgcatttat aatatcttca tcttccactt caaattcata ctcgcatatg tacgccccaa
    13321 ttacactatg agacaaggaa cgatttccat cctctattgg gtctaaatat atttcatact
    13381 tatttacata atcctctatt tccttgtcac ttaaatactt tgcacaatca tgaatcattc
    13441 ctgctaagta agctttttct ttgtcacatc cataaatctc acataatttt tcagcacatt
    13501 ttgcaacatt ttttgaatgg ctaagcctcc ccactggtag catttgattt agtctttggt
    13561 taatattttc taagttcatt taatcatctc catattgata tagtttattt tcatataaat
    13621 aattttctac attttcgggt actaagtaac gtattgactt gccttcattt agctgttccc
    13681 ttatatatgt ggacgatata tccagtgatg gaacataaac tgttataatg tcagcattat
    13741 acttcttagt tagtttttct atagtctctt gtgaccttaa caaactaatt cctggtctag
    13801 ttgcggctat aaaagtagct aactcaaagt ttttctttac atctttccac tcttcaacac
    13861 tacaaattgc atctgcacct gttataaaat aaatgtctgc atttttgtat tttttcttta
    13921 agtattttaa tgtatctatt gtataagtcc ttttctttct ttcgatttct acttttgaaa
    13981 ctaaaaaatc ttcgttgctt tcagttgcta gaagtgtcat attatatcta tcatacttat
    14041 ctgtagtgat acataattta tgtggtggat ttcctgatgg tataaagatt attttgtcaa
    14101 tgtcatactt gtctctaata aattctgctg ttgctaaatg agcatagtgt atagggtcaa
    14161 aagtacctcc caatatacct attttgattt tgcctttatg gcgattaaat ttctctagtt
    14221 tttcagtatt tttcaactct gccatcttaa ctagattttc acttctcata ttttgtctcc
    14281 ttttaatgtt ataatactat tgtaccatta ttttttattt cttaaagctt ataatttgtt
    14341 tttttgggta tataattata tttatatgct aaaaaacact ttaaaa
//
LOCUS       DAELNY010000093        14299 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.093, whole genome shotgun sequence.
ACCESSION   DAELNY010000093 DAELNY010000000
VERSION     DAELNY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.093"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..1682
                     /locus_tag="KPE55_002295"
     CDS             192..1682
                     /locus_tag="KPE55_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0493355.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1962..3458
                     /locus_tag="KPE55_002296"
     CDS             1962..3458
                     /locus_tag="KPE55_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0493356.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3603..4529
                     /locus_tag="KPE55_002297"
     CDS             3603..4529
                     /locus_tag="KPE55_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493357.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDSKTLSVNQ"
     gene            4647..4838
                     /locus_tag="KPE55_002298"
     CDS             4647..4838
                     /locus_tag="KPE55_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493358.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            5061..5411
                     /locus_tag="KPE55_002299"
     CDS             5061..5411
                     /locus_tag="KPE55_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493359.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            5673..7178
                     /locus_tag="KPE55_002300"
     CDS             5673..7178
                     /locus_tag="KPE55_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG0493360.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            7568..8665
                     /gene="dinB"
                     /locus_tag="KPE55_002301"
     CDS             7568..8665
                     /gene="dinB"
                     /locus_tag="KPE55_002301"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG0493361.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            8935..10071
                     /locus_tag="KPE55_002302"
     CDS             8935..10071
                     /locus_tag="KPE55_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493362.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            10064..11611
                     /locus_tag="KPE55_002303"
     CDS             10064..11611
                     /locus_tag="KPE55_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0493363.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            11945..12811
                     /locus_tag="KPE55_002304"
     CDS             11945..12811
                     /locus_tag="KPE55_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0493364.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            12952..14094
                     /locus_tag="KPE55_002305"
     CDS             12952..14094
                     /locus_tag="KPE55_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0493365.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         5576 a         1474 c         2602 g         4647 t
ORIGIN      
        1 agattgaaaa gtaaaaataa aaatagtgaa atttcagaaa tatcaataat attatataat
       61 aaaacaaaaa aacactatat atattcgata aaataaaatt taaacatata atattttatg
      121 tatatattaa taaaaggaca tattgtgtta taatggtatc gtataaaatc atttacgggg
      181 gtggtattaa aatgtttcaa tttgaaacac aaatacacaa attaagacat gatgttttaa
      241 ctgcaatagc tagacttgga aaagaagata accttaccag agaagcaatg gataatattc
      301 agtatgaaat aataaaaggt gagaaaccta catacagatg ctgtgtttat aaagaaagag
      361 ctataatatc tgagagagta aatgtaagta tggggttaca accaggtcaa gataagatag
      421 atttagatac aatggatata gaagaagatg aacaaattat atatattttg gaagctgcat
      481 gtgacagttg tccaataaat gaatttacag taacagatat ttgtagagga tgtctagcac
      541 atagatgtaa agaagcttgt aaatttggag ctataacaca tgtcggagga atggcctata
      601 taaatcatga attatgtaag gcttgtggaa tgtgtaaaaa agcttgtcaa tatgatgcca
      661 tctcagaggt tgttagacca tgtaagagtg tttgtccaac taatgcctta ggatttgata
      721 gagaaaatat gaaagctatg atacatgagg ataaatgttt aaactgtgga gcatgtatgt
      781 cagcatgtcc atttggagca atatctgata agagtttgat agctcctgta gctagaaagt
      841 tagttcaaaa agagaaaatg tatgcggtgg tagctccagc tataacaggt caagttgaag
      901 caaatgttac atatggtcaa ataaaaaatg ctataaaatc actaggattt gttgatgtat
      961 tagaagcagc ttgtggagca gatgcagtta cagttcatga aagcttagaa tttgctgaaa
     1021 gaatggaaaa gggagaagct tttatgacaa attcttgttg tccaggattt gttggttata
     1081 tagaaaaaac atacccaaat catgtaggga aaatatcttc tacagtatct cctatgatag
     1141 ctagtggtag atttataaaa agtcatgaac cagatgctaa agttgtattc ataggaccat
     1201 gtacagccaa aaagagtgag gcagctaaga aagaattaaa aggagctgtt gattatgtac
     1261 ttacttttga agaaataatg gcattatttg aggcatttga tatagacttg gctaagtgtt
     1321 caaatgaaga tatagatgat gcatcaattt ttggaagagg ttttggagct gctggtggtc
     1381 ttacaaaagc tatagaaaat tatcttgctg aaaaaggtat tggtgttaat tttgaacctg
     1441 taaagatttc tggttcaaga gaaatcaaga aaactatgac tatggctaat ttaggaaagc
     1501 tagaaggtaa ttttatagaa ggaatgatgt gtgaaggtgg ttgtataaat ggttctggta
     1561 aattcttatc aggtgcaaag gttaaagcca cttttgatag aaaaaattct caatcaacta
     1621 agaaaagtgt cttagcaaat acaaagataa aagattttag taatataaat ttagaaaaat
     1681 aaagataata caaagcaaca aaaatacata ttgttaatag agttgttcta agttgaggtt
     1741 taacttttgg acaactctat ttttatttat atatttaatt attgaacaac taaaataaaa
     1801 attatataca cttaaaataa aaattacata tataatattt tgtatataac ttaacaaagt
     1861 atatgcctat gttataataa tttatagaaa taaataaaaa atacaatata cataatagta
     1921 gaaatatagc aaaaaggtgt gatttatagg gaggattact tatgtttgaa tttgaaacgc
     1981 aactaaaaag gttaaaacat gaagttttaa cagcagtagc aagactatct attgaagaaa
     2041 atttaacaaa agaaaatata gaaaaaattc catatgaaat aattaaagga gaaaaggcga
     2101 gatatagatg ttgtgtttat aaagagagag aagtagttct tgaaagggcg caacttgcat
     2161 taagtttaac tccaaatagt aaatatggag atataaatcc agattcagtt agtttggaca
     2221 cagatgagca aattatatat atagttgaag cagcatgtga tagatgtcca ataaatgaat
     2281 ttacagttac agagctttgt agagggtgtt tggctcatag atgtaaggaa gcatgtaaat
     2341 ttggggcaat ttcttatata aatgggagag catatataaa tcatgattta tgtaaggctt
     2401 gtggaatgtg caaaagttca tgtcaatatg atgctatatc agaagttgta agaccttgta
     2461 aaagcgtttg tccaacaggg gctttggatt ttaatagaaa cactatgaaa gcaatgatac
     2521 atgaagataa ttgtataaat tgtggagcat gtatgtcagc atgtcctttt ggagcaatat
     2581 ctgataagag tttgatagca cctgttgcaa aaaaacttgc aaagaaagaa aatatgtatg
     2641 caatagtagc tccagctata acaggtcaaa tagatagtaa tattacatat ggtcagttaa
     2701 aaaatgctat aaaatcactg ggatttgtag atatgataga agcttcttgt ggagcagatg
     2761 cagttacagt tcatgaaagc aatgaactta ttgaaagaat aaaatcagga gataattaca
     2821 tgacaaattc atgttgtccg ggatttttta agtatataga gaagatgttc ccagatgaaa
     2881 aagatagaat atcatctaca gtgtctccta tggtagcaac aggaagatta gtaaaaaaaa
     2941 tagattctga tgctaaagtt gtatttatag gtccatgtac agctaaaaag agtgaggcaa
     3001 cagagaaaag tttaaaagat tcagtagatt atgtacttac ttttgaagaa ctaatggcat
     3061 tatttgaagc atttaatata gatttatcaa aatgttcaaa tgaagatata aatgatggtt
     3121 cgatttttgg aaggggattt ggagcatctg gtggacttgc caaatcagta cagaactata
     3181 ttgctgaaaa ggggcttagt tttgattttg aacctataaa agtatctggt ccaagacaaa
     3241 ttaaaaagac aatgacaatg gctaaattgg gaagattaga aggtaatttt atagaaggaa
     3301 tgatgtgtga agaaggttgt attaatggag caggtaagtt tttatcaggt gtaaaagcta
     3361 aaaatacttt tgatagaaaa aatggacaat ctactaaaaa gagtgtttta tctaatgata
     3421 agattaaaga atttgaagat gtaaatttag aaagataatt gttttggtac agtcattttt
     3481 ttatcatgtt tttatcatgc cacaaatatg cctaaaattt gcccttttta tatgtgtatt
     3541 ttacagagtt aaggttgcta tactataaat atgtaataaa taaaatttat aggagctttt
     3601 ggatgaggaa aatagtatta aaagatatcc tgtttttagt tactacatta tctataatag
     3661 taatattatt gcttgtaaca aatataacta ctaatagtaa gataaaatca acaagtgaat
     3721 cttatttaaa aagtgacaaa aatagtgtga ggcaaaatgg tatccagagg agcacaaaga
     3781 gtagcacaaa aaatactgca tttaagacta ctatactaaa cactggaaag tctgattgca
     3841 tattggttga gataggtaac aaagtcataa tgattgatac aggagaagat aaaaatggaa
     3901 aacaaatagt ggataggtta aaagaaaaag gaataaatac acttgactat ttaattttaa
     3961 ctcatttaga taaggaccat ataggtggag tagatagtgt gttatctagt gttaaagtaa
     4021 agaatattat acaagcaaat tataagaaag atagtaagca atatgatgaa tatattgact
     4081 cacttaaaaa agctgatata gaaccagtgc tactaaaaga taatatgaat attgtaatca
     4141 atggtgcaga aataaacata catccggctt caaaaagcaa gtacgagagt tcaaatgatt
     4201 attcaataat cactaatata agctatggag cacataagtt tttatttgca ggagatgcag
     4261 aggaagaaag attgtcagag tttataaatg gaaatacttt aaaatatgat tttgttaaaa
     4321 tgcctcatca tggaagatat gacaaattaa ctgaaacatt tttagagtct atttcacccc
     4381 aatatgctgt tattacatgt tcagagaaaa aagaaccaga ggaaggtgtt ttaaaaatat
     4441 tggaaagatt gaatataaaa acatttttga cttctaatgg agaggtagtt atcaatagtg
     4501 atagtaagac attgagtgtg aatcaataat ttttttaaaa atatctaaaa aaatttaaaa
     4561 aaatcattag caaatattac cccttaaaca tagtatattt taggagctga aactccacat
     4621 acaaaatgac taggaggtaa catattatgt tggatagatt ttttggtgaa ggcggatttg
     4681 gtggatgtgc ttggtggata ataatattat tcttcttatt cttagcattc caagattgtt
     4741 gggctgaaat ggacttatgt gcatggatac ctttcttaat attattatta atactttgtg
     4801 aatgtggtga cgtttttgat ggaggatgtg gatgctaaaa aaatcaattt agatgattta
     4861 ataaagctta tggtgggtat aaataaaata ccaaaccaat cacaatctag tgtttgaagg
     4921 tagtcggaaa tcccccgcta ccttcttttt tttattgtta aaatttattt ttgaattatt
     4981 tcatttacat ataataattt ttacgataaa gtctgatata attaaaaaga aaataaaatt
     5041 tatttatagg agatgtaaaa atggctatag tggataagat tttcaatgag ataaaactta
     5101 tggttgcaag acttttattg ggaaaaaaat acagtgaata tgaagaaaaa gatttagtgt
     5161 ataatacaga agaagaagtt atacttataa ctttaaaaag attagttttt cagggaaatg
     5221 taaacgaagc agaagaaatt ttatttgata aagctaagag tgtaaatagt gaaaatatgc
     5281 agtatatagc aatagaattt tatactatgc ttatggaaaa aactgatgaa gaattagaag
     5341 ctatgaattt ttctaaacaa gaagtttatc aaggtataga agatattaga aaagtattga
     5401 atttagaata aaagatatat gaaaatattg aaataaatta atgctaaggt gagaatatta
     5461 tacttatata taaaaatata gtattcttat tttattagtt atattaggta tatcttattt
     5521 accataaaac tatcttgaaa aaaattaatc ttaattgtat aatattactt tgagtgaaaa
     5581 agtattaaca tttttttata agatgtttta ctaaaaaaat atatataaat atactgttat
     5641 caataagaaa tttaatttag tgaggtgtta agatgaaagt aggatttgac cacaaaaaat
     5701 atttagaaga gcagtctaaa tatatcttag aaagagtaaa taattttgac aaactttatt
     5761 tagagtttgg aggaaaattg atgggagacc ttcatgcaaa aagagtactt ccaggatttg
     5821 atgaaaatgc aaagataaaa gtattacaac atatgaaaga aaaagttgaa gttgtaattt
     5881 gtgtctatgc aggagatata gaaagaaaca aaataagagg agactttggt atcacttatg
     5941 atatggaagt tttaagatta atagatgatt taagaggtta tgaactagat gtaaatagtg
     6001 tagtaattac tagatttgaa ggacaacctg ctacaactgt atttataaat aaattagaac
     6061 gtagaggaat taaagtttat aagcattatc caacaaaggg atatccgtca gatgttgaga
     6121 ctatagttag tgatgaggga tatggagcaa atgaatatat agaaactaca aagccaatag
     6181 tagtagtcac agcaccagga cctaatagtg gaaaacttgg tacttgtcta agtcagttgt
     6241 atcatgaaaa taaaagaggt aatgtagttg gatattctaa gtttgagaca ttcccagttt
     6301 ggaatgtacc tgtaaaacat cctttgaata tagcttatga agcagctaca gttgatttaa
     6361 aagatgtgaa tatgatagat tcattccacc ttgaaacgta tggtgaaatg tcagtaaact
     6421 acaatagaga tttggaatta tttccagtgc ttaaaaaaat aatagaaaag ataactggaa
     6481 aagaatcaat tttccaatct ccaactgata tgggtgttaa tagagttggc tttggaataa
     6541 tagatgatga agtaataaga aaagcttcta tagaagaaat aataagaaga tactttaaaa
     6601 ctgcctgtga gtataaaaag ggacaagttg ataaaggtgc ttatgataga atgaaaatga
     6661 ttatggaaga gcttaactta aagccagaag atagaaatgt tgtattacct gctagaaact
     6721 acagtaataa attgaaagaa agtgccgaca agaatgatac ttgtcctgta gttgcattag
     6781 aattagaaga tggtacaata ttaactggta agggttctga tttaatgaat ggaaccgcag
     6841 cagtagtatt aaatgccatt aaacatttag ccaatatatc tgatgatatg cacttaatat
     6901 cacctgtaat attagaacct atcataaact taaaaacaaa gactttagct agtagaaatg
     6961 tttcacttag ttgccaagag gtgttaactg cacttagtat ttgtgctgta actaatccaa
     7021 ctgcacaatt tgctatggaa aagttagcat tattaaaagg gtgtcaagct cattctacta
     7081 ctatattaaa tagagatgat gaacaaacat ttagaaaact tggtatagat gtaacttgtg
     7141 acccagaata tccttcacaa aatctttact atagttaaaa ttatataaaa aaatatgggc
     7201 tgttttaaag taatagtttg agcagtccat tacaattata ataaaatcag taggctgtct
     7261 tgtataagac agtcttttgt ttttaaaaca tcccataaat taaatatata tactgttatc
     7321 cataaggaat tttaaattta gttccttgga atacctattg taaagctttt atatagtttt
     7381 tgcaagtgtt tttaaggatt ttttataaat tatattgaag ttaatggatt atagatggtt
     7441 ttatttatgg aaaatatgaa tatataaaga aaataatgaa aaaataccaa aaagaaacat
     7501 aagtttggta taattaaaaa gtaatataaa taaaattaat ctatttatac tttaaaaggt
     7561 gataataatg aaacattata gaaaaataat tcatatagat atggacgctt tttatgcatc
     7621 tatagaacaa agagataata aaaaattaaa aggaagacct gttattgtcg gtggaaatcc
     7681 acaaagccga ggtgtggttg caacgtgttc ttatgaagct agaaaatttg gtatccattc
     7741 agctatgcca tcagctgtag cttacaatag gtgtccatat gcagtttttg taagacctag
     7801 atttgatgtt tataagagtg tgtcagagca aataagagat atattttata gatacacaga
     7861 tttagttgaa ccattatctt tagatgaagc ttacttagat gtaacaaaaa ataaaaaaaa
     7921 tattgattca tcaattgaga tagcaaagca aataaaaaaa gatatattta gagaagttgg
     7981 tcttacttct tcagctggtg tgtcatacaa taagtttctt gctaagattg catcagactt
     8041 aagaaagcca aatggattaa ctgtaataac cgaagaaaat gcccaggact ttttagataa
     8101 attacctgtg aataaatttt ttggtgttgg aaaagttact tcaaatactc ttaaaaattt
     8161 aggtataaaa actggatatg atttaaggtg tttaaattta tttgaacttg aaaatatatt
     8221 caaaaaaaga ggttatgaat tatataaatt tgctagagga atagacgata gaccagtgga
     8281 accaaatagg gttagaaaat ctgttggggc tgagactaca ttaagtcata atttggatat
     8341 tgatgaagaa gaaacacgca acattcttga tgaactttgt gaagaagtat gtcatagatt
     8401 aaaaaactct gagaagtttg gaaaaacatt aacattaaaa attaaatatg aggactttac
     8461 aaagataact agaagcctta gtttagaaca ctatattgac gaatataatg atataagaag
     8521 tggtgtagat aatttgttga gaaatgttga ggttaatgga aaacaaatta ggctattggg
     8581 agttacaata tcaaacctaa gtgataaaaa agaaacttat aaagatatta cattatttga
     8641 atacatggat agtatccaaa tgtgagtttt gtttaaatga aataacacta gctttaatac
     8701 tatataattg aggctagtgt tattttatga atatttttat attttatatt ttttgttttt
     8761 tgacattata tattatatac tatatataaa tactttatat ttgtagcaat attcagacaa
     8821 atagtgacct aaaaatcgta ctttgtttac ataaataaaa tagtatgata taattataaa
     8881 aacaacttat aatattgaag aaggttgctt gttcgaaaaa ggggatgata aattatgata
     8941 gagataagaa atgtaacaaa aaaaatagga aataatgtta tattagatga tatttctcta
     9001 gttgtagaaa caggtacatt ggtagttttg attggttcaa gtggttgtgg aaagactact
     9061 actttgaagc ttataaacaa attaataaaa cctacatctg gtgagatata tattaatggc
     9121 aaaccaattt ctcaggaaaa tgaaatagag cttagaagaa aaataggata tgttatacag
     9181 aacacaggcc tattccctca tcttacaata aaagaaaata tagagctaat tccacgccta
     9241 aaaaaggaaa aatccgttga agaaattgaa aaaagaacgt tgcaactact tgagatggta
     9301 ggacttgata gtgatgaatt tttgaataag tatccttcag aacttagtgg tggacagcaa
     9361 caaagaatcg gtgttgcaag agccattgct actgatgctg aaataatatt gatggatgag
     9421 ccttttagtg ctttagaccc aataacgaga acatctttac aagaacaatt attttctctt
     9481 caagatgaat tgaaaaaaac tataatattt gtaactcatg atatggatga ggctttaaag
     9541 atagctgata aaatttgtat tatgaaggat ggaagaatag cacaatatga tactcccgaa
     9601 aatatactta gaaagcctgc aaatgacttt gtaaaagatt ttatcggaga agatagagtt
     9661 tgggataatc cagaatatat aaaagcaaga gatattatga taaaaaatcc tattgctgtt
     9721 aattctacta gaactgtaac tcaaggaata gaaattatga gaacgagtaa ggttgatagc
     9781 ttactaatta tagatagagc aaaaacatta aaaggtattg ttactgtaaa agatatgaaa
     9841 gatattgatg ataagtcaat attacttgct gatattatgt caagtgagcc tttgcatgta
     9901 aatgaaggtg ataatttagt agaaatacta aatgtaatga atcgtaattc ggttggatat
     9961 attccggtta tttctgatga aaataaactg gtagggctta ttactagaag tagtttactt
    10021 tctgttttaa gtgaacaatt cttagaaatg gaggtgagtg ttcttggcta atttttttaa
    10081 ctttatacta ttacaaaaag ataagattat agagctttta atacaacata tgagtctaac
    10141 tgttacatct attttaatag ctataatagt tggtgttcct cttggaataa ttatttcaag
    10201 aatctcatct cttagaaagt ttgtacttgg atttgtaaac ttagtccaag cagttccgtc
    10261 tatggcactt ttgggtcttc ttgtacctat acttggtata ggaagtaaac cagccatatt
    10321 tatggtagtg gtttattcat tactaccaat tgtaaaaaat acgtatatag gtataacaag
    10381 tattgaccca gtagtattag aatcagctaa aggtattggt cttacaagaa atcagacatt
    10441 atttaaaata caatttcctt tagcgcttcc tataataatg ggtggagtta gaatatctgc
    10501 agttacagct gttggtttga tgactcttgc agcatttata ggtgctggtg gtttgggata
    10561 tttagtattt tcaggggttc aaactgtaaa taataatatg attttagcag gggcaatccc
    10621 atcatgtata attgcactgt tagttgatta tttatttagt aaaattgaag ttgcagttac
    10681 tccaaaagga ttaaatccaa aagcacctaa gaagaattat atagcactta aagtaatatc
    10741 tgtagtggtt gttgtttcaa tgttgttcgt tgtttttagt tctagttttt caagtaaaaa
    10801 agatacaata actattggtt ctaaagatta tacagaacaa cttatacttg gtaatgttta
    10861 tgctgaatta gtagaaaaaa atacgaatct taaagttaag aagaatttaa atctaggtgg
    10921 ttcatctgtt gcgtttaatg caattaaatc tggtgagtta gacatgtatg ttgactatac
    10981 aggtacactt ttggtaaatg taatgaaaca tgctccgatt aaagatgctg atgaagcata
    11041 taatgttgta aaagatacta tggaaaaaga aaatcagtta actttacttg accctcttgg
    11101 atttaataat acttacacat tagctatgat gcctgaaact gctgaaaagt atggaataaa
    11161 tactatttct gatttgacta aatatggtaa agaatttaca ttctcaccaa ctttggagtt
    11221 tgaaaataga gaagatggtc ttgtagggct ttctagagat tatggtttaa aattcaaaga
    11281 tgtaaaagca atgaatggaa gtttaagata tacagcttta gataataatg aatctcaggt
    11341 tatagatgca tttttaactg atggtcttat taaaaagttt aatcttaaga tattagagga
    11401 cgataaaaat ttctttgtac catattatgc agcaccatta gtaagagaag atactttgaa
    11461 gaaatatcct gagttagaaa aagttatgaa tatgctttca ggaaaaataa atgaagaaac
    11521 tatgagagaa cttaactatc aagtggatga acttggtaaa tcaccagaag aagttgctca
    11581 ttcattctta gttaaagaag gcttagtata aaaaattaat agtttatatc aaaaatttaa
    11641 gggagtgtct caaaatgaac tttttagttc atgaggcact cttttttata tgaaatcaaa
    11701 tatattttat catttcaaca ataaaaaaga agattatctt tattttgaga cagtctctta
    11761 tttttatgta taccaaaacc atacctacga tttttattta taattaagtt tcaaatagaa
    11821 tgaacaagta caaccaattt tgaatataaa tatagtaaat gtgcttatat acgattttat
    11881 tgtctggttg attgatattg atagatagct ttctttattg aatcgaaggg agggggaatt
    11941 tgttatggat aactataaga aagtgaagca agtattgtgg ataattttat ttgctaattt
    12001 tgcagtagca ttattaaaaa tcattattgg taatcaaata aaaagctata gtatgacagc
    12061 agacggattt cattctttgt cagatggagc atctaatata gtggggctta taggtatttt
    12121 ctttgcatct aaaccaaaag ataaaaatca tccttatgga cataaaaagt ttgaaattat
    12181 aaccagttta tttataagtg gtatgttgtt tgttatagca attaagataa ttcttagtgc
    12241 agtgttaaga atagctaatc cagttgtacc tgctataact atagaaagtt taattgcttt
    12301 aattattaca ctttttataa atatttttgt atgcatgtat gaatatagaa ttgggactaa
    12361 actaaatagt tatgttttga tttcggattc attgcataca agaagtgata tatttgtgtc
    12421 tctaggtgta ttggttactt tagttggtgt taagttagga tttccagtaa taatagaatc
    12481 tatagttcct attattattt ctgcattcat aatttattcg gcttatggaa tatttaggcc
    12541 atctattgga attttggtcg atagagtggc tgtagatgag gattatataa aagaaattgt
    12601 gtttgaattt aatgaggtaa gggatgttca caatatcaga agtagaggta gcaaaagttc
    12661 tatttatatt gacatgcatg taatggtaga tccatttata agcgtagagc aatctcatga
    12721 tttaacacat aaaattgaga aacaaataca agaagaaatt aatgaaaatg ctcaagtaat
    12781 agtccatatt gaaccttttt atagttttta acttagttat aaaatacaag ttataaaaaa
    12841 atataattta taaaaaaaat attataccat aaataattta taaaaattat ttatggtata
    12901 atattttcta taaagaaatt aagtcttaat atatctaagg aggaatttac aatgagatac
    12961 gttgaatatg gaaagacagg gaaaatggtt tcagtagttg gctatggagg attaaggttt
    13021 gatttagaaa aaagtaatca agaaaatgct gatttagtca aatatgcgta tgaaaaaggt
    13081 attaattact ttgatacagc accagggtat tgtgatgaca gaagtgaaga tatatttgga
    13141 ctagccttta gacaaatgat aaaggaaggt aagaccgatt tttatgtatc aactaaagaa
    13201 aagccaaaag catgtgatac atcagaaaaa gctatagagg ctgtaaaaaa atcaatagaa
    13261 cgattaggcg tatctaaaat aaatttttat catgtttgga atattagaaa gatggaacat
    13321 tacgaactag caatgagacc tggaggtcaa tatgaaggat tattgaaatg taaagaagaa
    13381 ggattgatag accatatagt attttcatct catcaaccag gagatgaggt tatagatatt
    13441 ctaagtgaaa ataagtttga aggtgtaact atgggaataa atatattaaa ctttccatat
    13501 aggctaaaag gtgccaaata tgctgttgat aatggatatg gtgttgtggc aatgaatcca
    13561 ttaagtggtg gaactattcc aaagtataat aaagaacttt cttttctatc aaaagatggc
    13621 gaaagtacta tagaatcagc tttaagattc aacataggaa tttcacaaat aagtatatct
    13681 ttgatagggt ttaataaaaa acaagatata gatgatgcat gtaaaattgc agacgaaaat
    13741 agaatttatt cagatgagga tatacatgat attgagacta gattaaataa gaatatgaac
    13801 gaaatatgta caggttgtgg ttattgtaaa gtatgtccaa aaggtattaa taccccagcc
    13861 tatatgcttt tttataatga aaagcagatg tttaaaaaga gtgatgaaga aatgacaaag
    13921 ttagtttatg gcttaggaca ttggaattac acaatgaata gtaaggcaaa agctaaagaa
    13981 tgtatatctt gtggaaaatg tgaagttgaa tgtactcagc atttacctat aatagataga
    14041 ttaaaagaaa ttaaaaaatg ggaagaagat ggagctaata gtgttaaagt ctaattaatt
    14101 ttatactata tttgaaattt gtatagtatt tagtaatgtt ttaaaatata gttttaatat
    14161 ttaaggagac agattataat tttgtctcct taaattatgc tttaatttgt tatagtttaa
    14221 ttccataagc aacattgtat atttttatat aaagtttttc aaatatttta cattaatatt
    14281 gcttacaaat tctcatttt
//
LOCUS       DAELNY010000094        14083 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.094, whole genome shotgun sequence.
ACCESSION   DAELNY010000094 DAELNY010000000
VERSION     DAELNY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.094"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..326
                     /locus_tag="KPE55_002306"
     CDS             <1..326
                     /locus_tag="KPE55_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493366.1"
                     /translation="LNIKNDKNVFIGESENWKAKFVTTEGHSKLEIKYKNSKDVVEQK
                     FNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLYSNRESSYKLIISWNGNEETI
                     NLKKK"
     gene            complement(1536..1787)
                     /locus_tag="KPE55_002307"
     CDS             complement(1536..1787)
                     /locus_tag="KPE55_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493367.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(2033..2188)
                     /locus_tag="KPE55_002308"
     CDS             complement(2033..2188)
                     /locus_tag="KPE55_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG0493368.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            2899..3123
                     /locus_tag="KPE55_002309"
     CDS             2899..3123
                     /locus_tag="KPE55_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493369.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     repeat_region   3410..3768
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3475..3503
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(4724..6151)
                     /locus_tag="KPE55_002310"
     CDS             complement(4724..6151)
                     /locus_tag="KPE55_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG0493370.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMSDGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSVAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTAAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYK
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            6342..7163
                     /locus_tag="KPE55_002311"
     CDS             6342..7163
                     /locus_tag="KPE55_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0493371.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            7288..8805
                     /locus_tag="KPE55_002312"
     CDS             7288..8805
                     /locus_tag="KPE55_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0493372.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            8828..9112
                     /locus_tag="KPE55_002313"
     CDS             8828..9112
                     /locus_tag="KPE55_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0493373.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(9209..10873)
                     /locus_tag="KPE55_002314"
     CDS             complement(9209..10873)
                     /locus_tag="KPE55_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG0493374.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(10945..12192)
                     /locus_tag="KPE55_002315"
     CDS             complement(10945..12192)
                     /locus_tag="KPE55_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0493375.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(12179..12853)
                     /locus_tag="KPE55_002316"
     CDS             complement(12179..12853)
                     /locus_tag="KPE55_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG0493376.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(13025..14083)
                     /gene="mltG"
                     /locus_tag="KPE55_002317"
     CDS             complement(13025..14083)
                     /gene="mltG"
                     /locus_tag="KPE55_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG0493377.1"
                     /translation="MKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIIDVPSGASV
                     GKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISLLVSGKIY
                     QDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKEDGITSLE
                     GFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVMEMASIIE
                     KEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKIDSPYNSYK
                     NKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKYVKEYKEA
                     RDKQSKDTKATNKENTKK"
BASE COUNT         4910 a         2139 c         1603 g         5431 t
ORIGIN      
        1 tacttaatat aaaaaatgat aaaaatgtct ttattggaga atcagaaaat tggaaagcta
       61 aatttgtaac tactgaagga cactcaaaat tagaaataaa atataaaaat agtaaagatg
      121 tagttgaaca aaaatttaat tattcctatc aatttataga tggtgaagat gcagttgtag
      181 ttgtaaaaaa tgaaatttta gaagcaggtc aagaaaaagc aataaaaata caaagaccag
      241 gtctttattc aaatagagaa tctagttaca agctaattat atcttggaat ggaaatgagg
      301 aaactataaa cttgaaaaag aaataaccat attatcaatt tttaaaaaat tattattcaa
      361 attcttataa aaagagtgtt tagaaagatg aaacactctt ttttgtataa gtttttgaaa
      421 actataaatg aaatatatca aaatcgttcg aaaaattact gaaataaact cttaaatata
      481 ttataaatta aggtatatta tacatgaatt tattgggagt ttatgagttt taaaattgga
      541 aaccaaacaa taagggcgaa aaatacttca aggaaaatat ataaagttct ataaattatc
      601 aaaaatattt cgcaaaaatt gataattatt acaaataaat tgataatatt agaaatatcg
      661 aaattgaaca aactaggatt aaagctcaat tagaaaaatc cactaatcct gaaaatactt
      721 tgttattctc atttattact gttgtttttt cattatttgc taatttattt ttcagttcat
      781 taaatgttaa atataactct ataaacttaa ttttacagtt aatatgtatt atagttctta
      841 tatttatata tattaataca tttttatatc gcctcttcaa tttgagcccc atcattatat
      901 aaaattaacc tacttgatat cttaatttaa tatatgtaca ctattcatat cttaatagtc
      961 aatattattc atttttaata gctcttttta gatgtcagaa ttattatatt ttgaaattta
     1021 ttaaattttt gcattaaaaa aactagaaat taatttaagt cttgaattac ttataacttt
     1081 ctacgtatct acctttatat tcttactctg attttatatc taatattaat tcttcatcaa
     1141 agctattaaa tatacctcta attgagttaa ttatttcact tacttcacct ttaattttta
     1201 tattaacttc gaccatttca aatctcatct ttttatctta taatttctat aagttttttt
     1261 atttattgtg ttgctacttt gaccagcact tttttattta atagtctaaa tgatattttt
     1321 taccagtttt aatatcctta aatactatat ttactaaaaa cttataattt cttttaatac
     1381 atagttaata tattcttatt actaataaca atactcaaca attttatcgc cttttttcaa
     1441 atatgattta acttttttat tcctaattat ttaaaaatag ttgtccaatt ttcttctatg
     1501 agaaagtgct aagtattagc aacttctcta ttctcttaac tttctactgt ctcaactttc
     1561 ttcatagcta tattaatagc tacttcttta gctacttcat tgaactcttc ccatcttctc
     1621 ctattttctt catcagatat acgtggatat actatataaa cttctgtgtt ttgattagct
     1681 ataatttttt catcatattc tatatttttt tctttatcat atttgtatgg tttaaattgt
     1741 tgtagtattt tcataacatc accccttgtt ataaaatatg aactcataaa cttgtcttat
     1801 acttatttaa ttgttacaat ttttacttaa aatcacttta tattctatat tttaaagcta
     1861 aatcctttac aaaagtaaca tattcttcta taagtttctt atcctcttac atagtatcta
     1921 gtttgtttaa cttatctctt ttaaacttac atacgccttc ttctgccatt cgtcttttat
     1981 taattgttaa ccttgtttca agagacacac caaaactctt gttaagaagc tcttagctct
     2041 cattagcaaa atattttaca taacttaaat caccttgact tctagctatt ttaactgtta
     2101 atagacttgt ttcttttttc caatgtgtta tatttaatat aacaacatct tttattattt
     2161 taacttctat ttttacatct aatgccatat tattgcttta ttaatttgta tccttaaatc
     2221 ttaaattttc tattaataca tcttctatac agcttagttt ctctcgctta atcttgaaat
     2281 atattttctc taaattgtca aattgttccc aagttttatc aatatctaat attttgcagt
     2341 gtctatttgc acatctttcg atccaaaggt ataattgact agaatgtttt gaaatcaact
     2401 gactattaat agattagttc ttaaattctt tcaaatactc gaattgtaat aaaacataat
     2461 ttacattctc tttatttcta ttgaagttag tttatatatt gtttacatca ttttcatata
     2521 tatctgctag ttgctatgca tttaaaattc tcttattatt tctttatatt acttgtaaat
     2581 tattcatcct catcatcctc ttaattaaat ttctatctta gaaatttaat ttgtaaaaaa
     2641 attttctata tcataatcaa gtatcttaga tagtttatag gtaacatatc aactctaatt
     2701 gaatattccc ctttcctgta cttaaaataa attgatgcat ttttaaaacc taactcattt
     2761 gcaacacttt gtaagtataa attttatgta tgtcttcttt tttataaaat catagtgtat
     2821 ctttttcata tattcacatt aattgctata tttcaataac aatatttcca aaacagaaac
     2881 ttttcgagat aattatgtat ggatgtaaat caaaggatta aatatattag ggaaaatatg
     2941 aatagttctc aaaaagaatt gcttgaaaaa acaaatataa atacaagtgt tatgaataga
     3001 atagagtctg gtgaaagacc aacaagagac gatgggttaa taatatttgc taaaattttt
     3061 ggtttatata ctgattatat tcttggttta tcagatacag aaaaactaaa tattggcaat
     3121 taaagctgaa ttaattgtat tatatgtgtg atgaatataa atagagaatt ttttgaaaag
     3181 cttgaaataa cttagaaaaa ataagaggtt cactcaagaa caagttgcta aagattaaaa
     3241 aacaacaaag gtatctacta aaagatatga aaatagttta agagagttga gtctggaaat
     3301 atttatgtgt attctttaaa taaaaattta ttttttaaat gataattaaa tttttactga
     3361 tatggctatg cttttaaaag tcctaacctt attttgatat tatatctgtc tttacatacc
     3421 acttaattga tataaaacag tataaaaatt agttacagat gtatcttttt tgttctttac
     3481 ataccactta gttaatataa aactattaat tttcctatgc ttgtactcat actcaaaaag
     3541 ctttacatac cacttagtta atataaaacg attgttattt tacgaaaggt acggtcgtaa
     3601 tttacgactt tacataccac ttagttaata taaaacaagc tagcggggat agacaatata
     3661 aatatcaaca cttctttaca taccacttag ttaatataaa acaaacggaa tatatatata
     3721 cagagcttag aggggaacac tttacatacc acttagttaa tataaaaccc caaaataaat
     3781 tgagcatttc caagacttac atctatacaa ttctctcaaa tttgcagtga accacgagta
     3841 gtgcaaatga taatatttat catataccct caatgcctta tgtttcaact gttaagccct
     3901 attttatcaa aaatatcgct cactgcaaaa ctatttataa tattatgaat atattatttt
     3961 taaatatctg taccaatttg tagtataata aaatcaagaa aataatttac tcctataaag
     4021 agtgattatt tttaagtgaa aatattattt ttttgaatca ttcttattgg cgtttgggag
     4081 atttttattt tatcataaat ataaactgat ataacaccag ctgacatgct tgctgctttc
     4141 gattattatt cttgctacaa ctattataac atataaaact tacttatttt taccaaatat
     4201 tttatgtaaa caataaatat taacttatga aatatctgct catttacaga tatttaataa
     4261 gttaatagct ctataataaa atttttatca ttgtttataa aaaattttac tttgcatttt
     4321 tttataaaca acagttttat ttataatgtt ttgtggtata ataaaagcaa gaagaactac
     4381 aatctatttt gcgttagagt ggagttcatc ataaacagag tttatttttt ggatttaaat
     4441 ataaatttaa attcaactcg taagtcactc tttgtgcgag agtggctttt tgcttttttg
     4501 aatagtttac tgattaagta aactaccaca ctagctgtta aactagctaa aacattaaac
     4561 aaaaagttat ccatgaactc acctcccttc gaatcgttgg gaggataatc ttttgtacat
     4621 gaactccact ctatagattg taagttaaat cttcttgcta aaaatattat aacatattat
     4681 ggtaaattat ataattaaaa acacttgttt aaaagttcta attttaatta taaactttcc
     4741 cagtttctat atctataaaa aaccaaccaa aagtaccatt actatttttt gaattgattt
     4801 taacagcata gacatttcca ctatatatat catctctaaa atctatcata tcaactacta
     4861 agttattgat actgacttta tgtctcttag cacattcttt caatgctatc tgttttgctt
     4921 gttcctctgt tatacttcct ttatttttat aatcatctgg attacatact ttcatattac
     4981 cattaacatc atatgtataa actttgctca tatcattttt agctacacag tatctaacat
     5041 caccctctgc accctgttta atgtaagcaa atacatagta ctcttttcct gaaaaatcaa
     5101 aatttttatc tagtagaaat attaaatctt catcttctga ataattaggt ttgtaataaa
     5161 atttcttcac acctaatagt tttttcactg cattagcagc atcttctact gtatattctc
     5221 ttccagtatt agaatcgcct atattttttg ttacatttaa acactgctgt aatatactat
     5281 cactcaaatt tcctatagca gttaatttag ttgtactttt caatacactt ttattactat
     5341 cattatcaac taacacaata gcattatttt tagcagcagt agaaccaaca agagcataca
     5401 ctaaatcagt accacttgct atataaaatt cttttactcc actataaaat ttatttatta
     5461 tctttttgtt agtatcatat ctatctgtcc cacctaatct agttgaatta gtatccttaa
     5521 ctattttgtc attcattgat gaagtaccac ctattacata actttctaca ccagttgtat
     5581 taaatggaac actttttcca tcagttaata ctatagctgc tttatctcta actgctacag
     5641 gtgcaacact cattgcatca ggctctcctt taaatgcatt agtcaatatg actttattta
     5701 ctttgttaat tgagtttatt tcttttgcga cattatagct tgtcttaatt ctatcactac
     5761 cttgaagtct tttaatctct attcctttgc cttttagaac agtttcagta tatttatcaa
     5821 tagagttttc tcctccaatt atatatactt tttttgcctt ttctaatctt ttaagtgtag
     5881 catttggtat gttatttttc ttagtcaaaa gaattggagc gtttatagct cctgcaagtc
     5941 cacttgcact taatccatca gacattgtac tgtctgcatt aattaatata gctgtagtat
     6001 agttttgttt gtctgcaatt ataccagcag tttcgtattt atctgcacct ttaattgatt
     6061 caattttatc aagagcattt gcagatatag ggcaactagc aataaatata gataaagata
     6121 accctattgt taataatttt ttaattttca taatatctcc ttctcttaaa tattttcacc
     6181 taaattatat aatacttttc aggaaaatgt taccataaat ttaaaaagtt attattactt
     6241 aatataatat taatgaaaaa tatcactcct aaattaatga ctttggtaga atatggtgat
     6301 aaaataaaat taatgagaga caatataagg gggattatat tatgataatc agtaaaaaaa
     6361 taacagcaat attagtatca tttttaatta tgatatcaat ggttggatgt gctccaaaag
     6421 aaagtgctac agaagtagct aaaaagtacc ttgatagtat aaaaatcaaa gactacgaag
     6481 tagcatataa tttattaagt aaagacagta aggaaagcgt cagtctagaa aaatttgaag
     6541 aatatcaaga tactttattt aaacttcaaa ccctaaaaga ttataaactt ggtgaagaaa
     6601 ataaagtaga gaaatacaaa tattctaata aagagtacac aaatcttgtt aaaataaaag
     6661 aaactgatac tataaagtat atcactgaaa ataaagaaga tactgcaaat tttgatagat
     6721 attttataat cgaagataat gaatataaat tattatggca taaagacttt aaaagagctc
     6781 ttagctctaa ttatgtcaaa atcgctaggt cagaaacaga aaaagatgaa gataaggatt
     6841 ataataaagc tgctattgat atcaaaaaag ctatagaaat tgatgataaa aatccacttg
     6901 catattatac acaatcatat atttatatag agttacaacg atatgatgag gctttaaagt
     6961 caattgataa atacttagaa tatctagata aaaataatga agcttataaa atagacgcta
     7021 gtgatgctta caatttaaga ggtgttattt ttatgagtaa atcagaattt aataaagcta
     7081 gagaatctta caataaggcg cttgaactta acccggataa tgaatatgca aaaactaatc
     7141 taaatgatat taaaggatta taaggcatta atattataga aaataagtat gttgaaagtt
     7201 aaattaactt ttgacatgct tattttctat attttaaact ctattgattt actatataat
     7261 agtgcataag gagtgtgatt acttattatg agtgttgcca tatacttaag aaaatcccgt
     7321 gctgatgaag aggcagaaaa acaaggcgaa tttgaaactt taagtagaca taaatcaacc
     7381 ctactaaaac tagcaaaaga acaaaactta gatgtaattg agataaaaga agaattagtt
     7441 tctggtgaaa gtataataca tagacccaaa atgcttgaac ttctaaaaga agttgaagaa
     7501 aataaatatg atgcagtctt agtaatggac ttagacagac ttggtcgtgg agacatgaaa
     7561 gaccaaggta tcatattaga aacattcaaa gaaagtaaga ccaaaattat cacacctaga
     7621 aaaacatacg acctaacaga tgaatttgat gaggagtatt cagaatttga agcatttatg
     7681 gcaagaaaag aattaaaact tatatcaaga aggatgcaaa gaggtagaat aaaatcagtt
     7741 gaagaaggga atttcatagg aacctctgct ccatttggat atgatgctgt aacaactggt
     7801 agaaaagaaa gaatacttgt accaaataag gatgctgatg tagttaggac tatctttgac
     7861 ttatatatta atgaagatat gggttgtagt aaaatatcaa aatacctaaa taacttaggc
     7921 attaaaactg ctacaggtgc taattggtac aattctgcaa ttacaaatat aataaaaaat
     7981 aaggtatatt gtggctacat tcagtggcaa aaaaaggact acaaaaaatc taaaaatcca
     8041 aataaaataa aaacagttaa actaagacct aaagatgaat ggattgaggc aaaaggaaag
     8101 catgaacctt taataagtga aataacatgg aaaaaagctc aaaatatact caaaaaaaat
     8161 ggtcatgtca gctatggtaa tcaaataaaa aatccacttg caggaatagt gatttgcaaa
     8221 aattgtggta gaccattagt atatagacca tatgcagacc acgactatat aatttgttat
     8281 catcctggtt gcaataaaag ttctcgattt gaatttattg aagctgccat tttaaaatct
     8341 ttagaagaca ctgtaaaaaa atatcaatta aaagcatctg atatagactt agataaaaat
     8401 aataaagata gtaatataga atttcaaaaa cgagtcttaa agggcttaga aactgaatta
     8461 aaagaattag gcaagcaaaa aaataagtta tatgatttac tagaacgagg aatatatgat
     8521 gaagatacat ttattgaaag gtccaacaat ataagttcaa gaactgaaga aattaaagat
     8581 tcaattaaga cagtaaaaaa tagattaagt actgttaaaa aagacaattc taaaataata
     8641 gaagatatta aaactgtgtt atcactttac catgattcag attcccttgg aaaaaataaa
     8701 ctcctaaaat ctgtaattga taaagcagtt tattataagt ctaaagaaca aaaactagat
     8761 tcttttgaat taatggtcca tttgaaatta catgaagacc agtaatacca ataacatata
     8821 aagagtagtg tgtaatgaaa taagtctttt agatatactt ggtacagagg ctaactatgt
     8881 tcttgatgaa gttgaactaa aagttcaagt tggaaaactt tacgaacaac taaacaaaat
     8941 tcttactcct agagaaagag aaattgtcca gttaagatat gggttaactc cttatggcta
     9001 taagacacaa agagaaattg cacaaaaact cgatatatca aggtcatatg tatctagaat
     9061 cgaaaaaaaa gctcttaaaa aactagaaaa agagcttgtt caagaaagtt aatactatat
     9121 tttaaaagga tatatcgtac atcattagac aaaattttct atctaaaatc aaagttacga
     9181 tatatcctta aattttatag tacataattt attttatact ttcacatatt tccttaaata
     9241 taggtgttgc agatttattt cctttttgag ttccttctac taacacagtt attacatact
     9301 taggtctttc ttcaggataa aatcctgtta tccatccatg gtctatagct tttttattta
     9361 aactactttg agctgtacca gttttaactc cacaaccacc ttcaatatct tttaggtctt
     9421 tagctgtacc aatcctagat acagacttca tatattcttt aacttgtgta cacacataag
     9481 gagaaataag ttcttctttt tttgaagact tataagtttt tattgtattc atattattgt
     9541 ccacaagact tttatataag tatagtggtt tgaatgttcc attattagct attatttgag
     9601 tcatttgatt tatttgaagt ggtgtaaatt ctatattttc ttgacctata gctaaatttc
     9661 ttattgatat gttttttgga gcttctctaa ttttttcttc atcaagacca atatcaacct
     9721 tttcaaataa atgtaatttt tcagcagatt tgagtatctt ttcttttcca agtttcatag
     9781 ctatatctaa aaatgcagga ttgcatgaat tagaaaaagc ttgtcttaag ttttggaaac
     9841 catgaccatc tcttttatta catcgcagta tttcattagt attcccaact ttagtcttac
     9901 ctgtacagtt gtaagtatag ttctcatcta taacaccatt ttcaagtgca gaaaataaaa
     9961 ctaccatctt aaatacagac cctggtggat atgtcgcttt tataactcta ttttcaaatt
    10021 ctccattttt tccttttaaa ctttttgata tgtcattttg gtcaaaatta ggtctagagc
    10081 acattgcaag aatttcacct gtagatgctt ccgagattat tgctgctgta ggattttctt
    10141 ctttatttag tatttgttct aatttttttt gaatgttata atcaatagta gtttttagat
    10201 gtctatcttt atccttatta tcaacagttt ttacacttcc ctttagtatg tttagagatt
    10261 tttcatttcc tgcatctcca gctttaaaag cacttatata cttttcattt gaatttctaa
    10321 gtacactatc catactcttt tcaattcctg attgacctga tttatcatct tcttttatat
    10381 atcctattgt atgtgataat aaaccatctt ttgcgtacct catagttttt tcttctacca
    10441 ttattccatt tttctcaagt tcttttttca tactcttatc gatattttta gtctgaatct
    10501 gtattatagg gcgtgtcaat tgctcttgta cagctttata tatatctaaa tcattcatct
    10561 tagtagcttt cttaataagc tccaaaattt tatagttatt atttaatttt tctttttcaa
    10621 caattaatac ctgagattta cttgtatctg taagcttctt gttgtttcta tcatagatta
    10681 ttcctcttcc actatttaac tcgactttct caacgctttg tgattcaaca ctttgtttgt
    10741 atttatcacc ttttaatact tgtatatcaa ctagcctata aattaaaaca ctataaatta
    10801 ttaaaattat agtaaatata caccagcttc tctttcccac ttttttcaaa aatggagtct
    10861 tcttttttga catgaaatca ccccttagat aatttcatta ttgacaattt ctaaggggct
    10921 aatactattt ttatcaattt tttattattc ttcaaccttt tcttctataa cttttctaag
    10981 catataatct ttaccaactt ttactgatag ctttaacttc actatttgtc taggatgagg
    11041 agctgattct atagcttctc cttcttcatt gtacatttct tcaattttag taaacatagt
    11101 ttctttatat ggtcccataa cttctatttc atcaccaaca cacattttat ttctttgctc
    11161 aacaacaata agaccatctt catcttctgt ttctctaact atacctataa aatcatagtt
    11221 tctaacatat gatgctgatt gataattttg gtcttctgtt gttggtttat tcaagtaaaa
    11281 acctgatgta aaatgtctat gactaccttt ttttagttct tccatccaca ttggattgaa
    11341 cttccagttt tctgggtctc tgtaaaattc atctattgcc attctgtatg ctcttacaac
    11401 agtagctaca tagtatgcag tcttcattct accttctatt tttaaacttg ttataccaga
    11461 tttgattaac tctggtatat actctatcat acataaatct tttgagttaa agaagaatgt
    11521 acccctttca tcttcgtata ttggaaaata ctcaccaggt cttttttctt ccaccaaatg
    11581 atattgccat ctacaagatt gagcacaaga acctttgttt gcatctcttc cagtcatata
    11641 gttgcttatt aaacatctgc cagaataaga aatacacata gcaccatgca taaacgcttc
    11701 tatatccata tctaaaggtg tttttgctct tatctcagat atttcatcaa aagataattc
    11761 tcttgccact acaactcttt ttattccatg ctcgtaccag aagtttgcac ttagataatt
    11821 ggttgtattt gcttgtgtac ttaaatgtat ttccatattc ggaattactt ttttaatgac
    11881 actaagcact cctgggtcag ctattataac tgcatctatt cctatctctt ctagttccaa
    11941 caaatagtct tctaattgta gaaaatcttc attatgaggt attatattta ctgttacgaa
    12001 tactttcttg cctctttcat gtgcaaaagc tactccctct gccatatctt ccttactaaa
    12061 attcttcgct gcagacctca ttccaaaaat ttctccacct atataaacgg catctgctcc
    12121 atatgttata gctattttca atctctccaa atcaccagct ggagctaaaa gttctacttt
    12181 atttaattcc atctacatca cctcttttac tttctttata acttagtgct acaccatcac
    12241 ctattggtac caatgatgtg cttaaatagt cacaattaca tatataatct aaataatttc
    12301 tcattctttt aactatagtc ttttttcttc ttacaacaaa atcatcatga gcaaccatac
    12361 ctttatacaa aatattatca gatactaaaa gtcccccatc ttttaattta tctataacta
    12421 aatcaaaaaa tagcttatat tgacctttgg ctgcatctat aaatataagg tcaaattcac
    12481 cttggacttg actaagtttt tcttctgcat ctccttctag tatagttatg ttattgtcaa
    12541 atccagctaa ttttatgttt tcttttgctt tttctatcat tttttcattt ctctcaacag
    12601 ttattatatc tgcatctttt cctataatag ttgcaaaaaa aatagaagaa taaccaattg
    12661 cacatcctac ttctaaaatt ctctttggtt tttgaacctt taacaagact tttaataaat
    12721 ctgaaacttc tttatgtatt ataggaacac tattttcctc tgcataaatt tctaaatctt
    12781 ttaaaaatcc ttctttttct tttaatgtca ctcttatata atcttcaacc aaatcattaa
    12841 ctatattact cataaaaaca ctccctaaaa taataaatat tcaagtttta tattaattta
    12901 ttatttttta aattcatatt gttatatata tttacacttt ctttattata aatctaagaa
    12961 taacaatttt caataaaaaa gcccaacatt attcttaccc tgttagactt tttataatca
    13021 tggtttattt ttttgtattt tctttatttg ttgcttttgt atcttttgat tgtttatccc
    13081 ttgcttcttt atattcttta acgtatttta aatggtcttg atagtttgta ctataattat
    13141 ttccaccatc aatcttagct acaaaatata aatagtctgt tttctctgga tataatgctg
    13201 cttcaataga ttttatacca ggatttgata ttggtgtagg aggtaatccc ttatttttat
    13261 aactattata tggtgaatca atttttaaat catcatatgt tacaattttc tttctctctt
    13321 caaatatata ttgtattgta gcatctgatt gaagaggcat acctacctta agtctattat
    13381 aaaacacact tgctattata ggtctgtctt tgtcaagaac agcttctttc tcaattattg
    13441 atgccatttc cattacttct tgaagagtca tatttaattc tttttgtttc tttttaaact
    13501 tgtccgtgta tttactgtca aatactttta acatttcgct taaaatatct tcttctgatt
    13561 gtttctttga attaaaataa taagtttctg gatacaagaa cccttctaaa gaggttattc
    13621 catcttcctt caaatatgga aatttatcat aaaattcttg aggttttttt attaaatttt
    13681 caaaagtagc tttatctcct aaatttttac ttactaacat tgctataatt tctttagatg
    13741 tagctccctc tggaatagtc acttttatac catcctgata tattttacct gaaactaaaa
    13801 gacttattat atcattgttt gaatacgatt gattaagtaa gtatgttcct gattttatag
    13861 acggagcttt attgctaacc tttaccaaaa gtttaaataa tagttcattc tttattaatt
    13921 tattttcata taatatgtca gatatttttc caacagatgc tccacttgga acgtcgataa
    13981 ttacatcttt tttgttattt ttatcataag gacctatttg tataaacaca aatatacctg
    14041 ctaagataat caaaataaca attattaata ctgctatttt caa
//
LOCUS       DAELNY010000095        14054 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.095, whole genome shotgun sequence.
ACCESSION   DAELNY010000095 DAELNY010000000
VERSION     DAELNY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.095"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            444..1514
                     /locus_tag="KPE55_002318"
     CDS             444..1514
                     /locus_tag="KPE55_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493378.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="KPE55_002319"
     CDS             1498..2367
                     /locus_tag="KPE55_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0493379.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="KPE55_002320"
     CDS             2411..3271
                     /locus_tag="KPE55_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0493380.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="KPE55_002321"
     CDS             3302..4606
                     /locus_tag="KPE55_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0493381.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="KPE55_002322"
     CDS             4669..5931
                     /locus_tag="KPE55_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG0493382.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEIVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="KPE55_002323"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="KPE55_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG0493383.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     IDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="KPE55_002324"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="KPE55_002324"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0493384.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="KPE55_002325"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="KPE55_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG0493385.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="KPE55_002326"
     CDS             13008..13937
                     /locus_tag="KPE55_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG0493386.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
BASE COUNT         5720 a         1613 c         2548 g         4173 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcgatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg acaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacgccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttttggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacata attatggatt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatagtgat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataatta tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aggaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagcaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgt
    10021 ataacaggtg tttcaggttc cggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gctttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat atcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat atta
//
LOCUS       DAELNY010000096        13897 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.096, whole genome shotgun sequence.
ACCESSION   DAELNY010000096 DAELNY010000000
VERSION     DAELNY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.096"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            299..1000
                     /locus_tag="KPE55_002327"
     CDS             299..1000
                     /locus_tag="KPE55_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0493387.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILL"
     gene            1026..1682
                     /locus_tag="KPE55_002328"
     CDS             1026..1682
                     /locus_tag="KPE55_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493388.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            1787..3427
                     /locus_tag="KPE55_002329"
     CDS             1787..3427
                     /locus_tag="KPE55_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0493389.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3785..5107
                     /locus_tag="KPE55_002330"
     CDS             3785..5107
                     /locus_tag="KPE55_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493390.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKLDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5304..6311
                     /locus_tag="KPE55_002331"
     CDS             5304..6311
                     /locus_tag="KPE55_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0493391.1"
                     /translation="MGKYQYSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6349..7761
                     /locus_tag="KPE55_002332"
     CDS             6349..7761
                     /locus_tag="KPE55_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG0493392.1"
                     /translation="MASTNKNNATSKEEEKEVSTSVLESCTVKEKKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            7905..9170
                     /locus_tag="KPE55_002333"
     CDS             7905..9170
                     /locus_tag="KPE55_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0493393.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9212..10810
                     /locus_tag="KPE55_002334"
     CDS             9212..10810
                     /locus_tag="KPE55_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0493394.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            10845..11867
                     /locus_tag="KPE55_002335"
     CDS             10845..11867
                     /locus_tag="KPE55_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0493395.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(12344..13747)
                     /locus_tag="KPE55_002336"
     CDS             complement(12344..13747)
                     /locus_tag="KPE55_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0493396.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT         4895 a         1664 c         2620 g         4718 t
ORIGIN      
        1 ttttaaaata ataaagtttt ttgattaaaa tttttatatt gaaatgtcaa aataataaaa
       61 gaccttataa gttaattaca ggttaaaaaa ataatatgtg aattttctga tatttactaa
      121 ttatgaataa aatatttaag tattaatgtt tttagtgaga ctttaagaca gataatctcc
      181 tattaaagat actatatctt agataaaata aattaaaaca atataaaaac ataacaagat
      241 tacagaaaga aagctcacaa taaaatgctg aaaaatttat tttaaggagg aataaagtat
      301 gaagtttcaa gaaatgatta atagcccagg tctttggatt gtttctagtt ttttagtaat
      361 tatttcagtt gttcaagcaa tcgtatttat gaaagaggca ctaaaagagg cttcaaatct
      421 tggtattgaa aggaaaggaa ttaaagcagc aattcgctca gctagtgtta cagccattgg
      481 accttctcta tctcctgtta ttacattatt atcactggta gctgtgattg gcgctcctac
      541 gacttggatg aggctctgtg atgtcggagc agctagaaca gagcttggag taatatctct
      601 tacatccaat ttatctgggg tagaagtagg tagtgctgca tttggagcag aagctttttc
      661 gtatgcacta tgggggatgg ctttaaataa cttaggttgg ctatttgttg tatttatttt
      721 gggtcataga atgcgtggaa ttgttgaaaa gatgaacatg aagtataatc ctacttgggt
      781 taaaaaacta cttgcaggag ctacagttgg attatttgct taccttcttt caaatcaaat
      841 aaaaacattt gaaataccaa aacttacacc agcaattatt tcagctatag ttatgctagt
      901 attgacaact ttatttaaaa agaatcaacg attacaagaa ttatccttag gtattgccat
      961 gttaattggt atgtttggta cacagattat attattgtga ttaaaacgga taagaggtga
     1021 taattatgta tgataaaaag atgataaaaa tagggagaat ttctataggt cttgcaatta
     1081 ttgcaaactt tattcccgct atctatgtag gtattcgctt tggtcagatg ccaatgcttt
     1141 ctgttatatt tcaaatttgg gggttagtcg ctgcgactta tggggttagt tggattgttc
     1201 aacctatagc ttattatcct acactaggag ctccaggttc ttatataggt tggcttgcag
     1261 gttctgttgg agatatacgt atgcctgcag catcaatggc acagaaaatt gcaggtgtag
     1321 aagcaggaac tcatgagggt gaagttgttg gaacaattgg tacatgttgt tcaatctttg
     1381 tatcagcttc tatgattacc atttttacca ttgtaggcta taaagtaatt cctttgttac
     1441 ctgaatttgt tactaattca tttaattata tacttccagc attatttgga gcaatttatg
     1501 ttgatattgc acgtaaggat attcgagctg gtggatgtac gattgctgta gcgttagcaa
     1561 ttatgtactt tggtgaaaaa attggtatac caggtggatt attaacattg tttattgtgt
     1621 taggtgggat acttatcaca agggtcttct ttgtaattga taacaataaa acaaaagaat
     1681 aggattgctt attaaggaga aagactagaa ttatttgttg agaaaaaaga ataggattgt
     1741 ttattaagaa atatgaatat aaataagtct tgaaaggaga aaaagcatgt ttgatgttaa
     1801 aattgtcaat ggaataatcg ttgatggtac aggaaattca agatataaaa gtgatgtagg
     1861 gattgtagga gacaaaatca tggctattgg agatttaagt caaaaagagg ctaaagagac
     1921 tatagacgca actggaaaat ttgtatcacc tggttttata gatttccata ctcattctga
     1981 tttatctctt gtgtatgata aatacacaag aagccgtatc catacaggtg taacaacaga
     2041 tgttattgca aactgtggta taggagttgc tccgataaga gaagagaaga agcaagagtt
     2101 aattgattat cttggaacta ggataattgg tacaattcca acaaaattag agcttcattg
     2161 gaatacaatg caagaatact ttgattactt aactgaaaat tctccagcag tcaatgttgt
     2221 agcttatgta gcacaaggac ctgtacgtat caatgaaatg ggattttcaa aggaaccagc
     2281 tactttagag caacttaaaa atatgaagtt ggaagttaga aaagctatgg aagctggatg
     2341 tgtagctctt tcatcaggat tagtttattt accaggagca tacacaaaga aagaagaaat
     2401 ggctgaatta tgtaaggaat tgataccata caatggatac tatatatctc atattagaga
     2461 tgaaggtgat gaagaaatgg aagctcttga tgaagcaatt tatatagcta agactgcagg
     2521 agttcctcta catgtttctc atttaaaagt tatgggtcat aagaattttg gaactattga
     2581 tgaggtattt aaaaagctag atgaagctga ggcagatggt ttggaagtaa cttttgattg
     2641 ttacccatat acagctggaa tgacatcttt gggtgcactt ttacctcctt gggcttttga
     2701 aggcggtgta gaaaatatgg taaaacgatt agaagtacaa gaaaatagag acagaattat
     2761 aaaagagttg gaggaaggga ttcctggatg gcaatgtttc taccaattag caggtgggtg
     2821 gaacggtgtt gtactagcct ctgtaatgac tgaagcaaat aaatatgtag agggtaaaac
     2881 tttaatggag gtagccaaaa tcaatggtga aaatccattt gatacgttct ttaggctttt
     2941 gattgaagaa aaaagtaaaa ttcaagtagt tgttcataca caaggtcaag aggatacaga
     3001 taaggttgtt tgccatccaa agtcatgtat aggttctgat agtatggacc tttctacaga
     3061 aggtttactt tctcttggaa agcctcatcc tcgtgcattt ggtacttttg gacgaatttt
     3121 ctcttactat gttagagaaa aaggtatgtt gacttttgag gaagcagtta aaaagataac
     3181 ttatctttca gcaaaacgct taggcattta taaagaaagg ggtctactta aagaaaatta
     3241 ttttgctgac attgtggtat ttgacccaga tacaatagaa gataaagcga cttatagtaa
     3301 tcctaaacag tatacagtgg gagtggaata tgtgttggtg aatggtaaaa ttgcattggc
     3361 aggaggaaag caaactgatg tatgtgctgg acgtgtaatt aaaaatcctc ttagcatagc
     3421 aaaatagaat acattaagaa cttttataaa aatagtcttt ctttgaatat taaggaaggc
     3481 tatttttgtt aaattaataa agattactct acttgtgttt tctaaagaat ttaactttaa
     3541 gtagaaaaaa caatatatta attgtaaaat gtaattaata ttaatgaata agatttaaat
     3601 acaaaaattt aaatacaaaa atgtttcaac agatagtcgt aattaaaaaa cttaaagtaa
     3661 ctttttatat taggtataat tgattattca aaattctatg aatcaggagt cctgtggctt
     3721 tagtcatggt aggtttagaa aatgttttaa gagataatag tatcattagg aaaggagaga
     3781 cgaaatgaaa atactttatg taggaccagg tatggtaatc gataaaatac tagcagtaag
     3841 taaaaagaat tttcctaata ttgaagcaga ttttcttaga tatgatagat atattgaagt
     3901 acctgattta ttggaaaaat atagaggtag agtggatgca gttcttttta caggtaagtc
     3961 gccatttaaa ttttttgaaa aaagacaaaa agattttgta ttgtctgatt atattcctag
     4021 acatgaaaca acattgtaca gagtgttgct tgaaattaca tatttgctta aattagatat
     4081 tagtagactt agtatagata cttatgataa ggctatgatg aagagattgt ataaagaaat
     4141 tggagttgaa tttaaggatg aacatatgtt ttttgcagag caaaagtatt tagaagagga
     4201 ctatataaag tttgtgcttg aatttcataa atataattat caaaataatg atgtatgttg
     4261 ttgtattaca ggtcttgaag aggcctatca tataatgaaa aaagataata ttccagtagt
     4321 tttagccttg ccttctgagg acgtaatggt gcaaagtata aaaaacattc agatgcgtta
     4381 tattgcaaag aaaaacaatg agaatcagat tgttgttctt gctattaaac tgaacatgcc
     4441 ttcagaatat tcactaatga aggaagatga gtacgcttat ctttcacaaa ggattaagat
     4501 tcttgataaa ctataccatt tcaatagccg tatagatggt gttttggtag agcagagtag
     4561 aagtgaattt atgattttta caactaaaaa aatacttgag ttagaaacaa agaaatacag
     4621 agatatttat ttgttggata tgattcgtga agtttcagtt ataaacactt atataggaat
     4681 tggttatgga aaaacagcaa atgaagctaa atttaacgct tatgagagta ttaagatggc
     4741 tcaaagtcaa aaaaatgatg cagcatatgt agtttttgaa aatggagaaa ttatgggacc
     4801 actaaagcca aatcctactg tcaaagagaa aaatagtttt gatgaacgtt tttatcaaat
     4861 tgctacagag acagggctaa gtgctaatac tgtatataaa gtattcaata aaataataaa
     4921 tagtggaaaa actgaattta catcaaagga attagctact atatgtggtg taagtattag
     4981 aactatggac aggattatac ttagattgtg tgattctggt tattgtgagg tagtgagtga
     5041 acagcttatg agcaagtatg gtagacccag tagaatatta cgttttaaac cttttctgtt
     5101 gttttaaata gatataagtc aaaaattaaa tttttatgat aacattttct ttacaatttt
     5161 ttagatatat gttaatattt agaaaaatgc atatgtattc ataaaataaa cttaaaacag
     5221 ctgaaaaatc attaaaaagg aaatcgtatg catgaaatga gaaagttaat ttataactta
     5281 aataaatctg ggaggagata attatgggaa aatatcagta ttcaaaaaaa gttgtaagaa
     5341 attattttga agcattagaa aatgctactg gggatgaggt tgaaaatgtc ttaaagcaac
     5401 atatgaaatc agattataac tggaaaggtg tgtatccatt tcgtgaacaa gaaggcactg
     5461 aaaatgtagc agatgtattt tggaagcctt taaaaaaatc attgagtaat atgcaaagac
     5521 gacaagatat tttcattgca ggaacaaatg agattgacaa tagtgaatgg gtaatgagta
     5581 tggggcattt tatgggactt tttgataacg attggcttgg cattaaacat actcgtaaaa
     5641 tgattagttt aagatatgca gaatttaact gtgttcaaga tggtaaaatt gctaaaactg
     5701 gattatttgt agatataatt gggtttatga ttcaagcagg agttaatcca ttgccaccac
     5761 aaacaggttc atattttgta tatccagggc caattgacca taatggatta ctttttgaag
     5821 atgcagatga agttgaagga gtaaaaactt tagcattagt aaataaaatg gtagatgatt
     5881 tatctgaatt aaatgaaagt ggtgctatgg gatgcccacc agaaatactt gaaaaatctt
     5941 gggctaaaga tatgatttgg tatggtccag gtggaattgg tgcttcttat actataccta
     6001 gatatcaaga gcaacaccag ctaccattta gaaataattt aaaaggaaag actttcaatg
     6061 ggcatgtatg tagatttgca gaaggtaatt ttgcttgttt ctttggatgg cctaatctga
     6121 ctaatgtacc atgtggaggt tttttaggat taccaggtgg agaggttaaa gcagatatgc
     6181 aagttgttga tgtttattat cgtaagggcg ataaacttca agaaaactgg gtgttaattg
     6241 atattcctta ttggttgaaa caacaagggc ttgatattct tgagagaact aaaagtattt
     6301 ttaatgctta gttatgcata cgtattctaa aacaaagggg gatataaagt ggcatctacc
     6361 aacaaaaata atgcaacaag taaagaggaa gaaaaagaag tttctacaag tgtgcttgaa
     6421 tcatgtacgg ttaaagaaaa aaaagctaaa atgaatcaaa ctaattttag taatataaat
     6481 gaaaatcaaa caaatgagaa taaaacgaac acatataaag gaaatataga taaaggaaat
     6541 atagataaaa caaacacaaa taaatccgac ataaataaag ttaacataga tacagtggat
     6601 acagataaat taaatacaaa tactcttact gaaaaaaata aaaaagttat atttaagggg
     6661 gaagataata tgtcatctac aaaaatttac tatggagatt cattagaagt aacgcctgtt
     6721 ggagtaatgg attacaatga tttttcaaaa cagacaaaaa gggaacagga aatacctgga
     6781 tttgattcta agtatagaga ttttgtagat tatattatga agattacaca taatatctgg
     6841 gaagaaaaag gtataggtgt tatttatgat acatatcaca ataatgttac aatgcattgt
     6901 ggctcttcaa atttagttgg tattaaagat gttatttcca atactcttca aacattacat
     6961 gcgtttcctg atagaagatt aattggtcaa aatgtaatat ggtctaactt tggtgctgat
     7021 ggttttttat cttctcaccg tgttttgtct acagcaacta acttaggtga tagtaatttt
     7081 ggaccagcaa caggtagaaa aataaacttt agaactgtaa ttgattgtgc cacaactaac
     7141 aatagaatcc acgaagaatg gttggttaga gataatttat ggattgttac tcaattagga
     7201 ttaaaccctc aggaagtagc aaagagtatg gcaaaggcaa gtgaatctaa agtgcttagt
     7261 ttacaatcaa cttatggtat ctgtgaatct atggatggtc aatttatgcc aactaagtat
     7321 caagcaaaag atgattctgt tggcgaaatg atgcttgaaa tgacaagccg tatttataat
     7381 tataaatata taaatgaagt taaaaaatac tatcatgata atgcagtagt acattttata
     7441 tgtgataaag atttaaatgg ctatgatgag attcaaggaa tgattgtaag tttacttgca
     7501 tcaattccaa atggaagtta tgaagtggaa agagtaactt gtaatcaaag agaaaaaaat
     7561 gaaggttatg atgtatctgt aagatggaga ttacgtggaa taaatgaagg tataggattt
     7621 tttggacagc cttctggaaa gcatatagaa gtaatgggaa tcaatcatta tcatatacta
     7681 caaggtaaag taaaggagga gtggataact tttgatggta tggatgtatt aaaacaaatg
     7741 tatatgggag tagaagaata aagctgttta gagtataaat agttttgaaa tgtatatagg
     7801 gaagtattgc tttataaaat ttaagtgggg aatgtatatg aaaaatagat gtttaaactt
     7861 ttagcaaaat tatgactaaa tttaaaaggg ggaattataa aaatatgaaa acaacggttg
     7921 aaatagaaaa tgatatcata ttaggaaaag actattcaaa cctaaaaaga ggaattgcat
     7981 ttttttcaat tgctttaata tacttttttt attgctacaa ttttatggtg ggtactttta
     8041 ttaaaccaac aatgatttat gctttagcag atgggggatt tggtttttca ttaaaacaaa
     8101 cagaagaaat atttgcagtt atgtcttttg gtacaattcc aggtacattt atttttgggg
     8161 tgatttctac aaaaattggg aagaagagga ctttaattag cgttgcttta ctgattggat
     8221 tgacaacatt tataccaatg ttatctccta ccaatattat gttatggaaa atagcacgtt
     8281 tgtgtacagg agtagtgttg ggaggcgtat ttggaacagc tatgccttta gttgcagata
     8341 tgtttccaag taaatatcgt ggtaagttag ctgctatatt aactgcttta ttttcattag
     8401 caatgatttt tggtgggaaa gtatatggcg ttttaggtga tgcaaattgg caaatattga
     8461 tgtatactgc gattatacca cctatagttg gagctatctt agcaatcttc tttgtgccag
     8521 atgatttgga gtatacaaag gaattagtta aaaagggaaa agaaactggt gaaaagatta
     8581 gctatataag tatgtataag ggaaaatact tatggattgg attgggaacg attcttttat
     8641 caggtgcaaa ttttacagca tattcagcat tttcaaataa tgcaactaca tacctagtga
     8701 caggaatagg aatgtcagca gcagtggctg ggtctatata tagtttacaa ggaattggtc
     8761 aattagtagg atatttattc tggggttcaa ttgcagataa atttggaaga aaaatacctt
     8821 ttataggtat ggcattagca gcagtatttg tttttatata tacaaaatta ggtggagata
     8881 atacaacaac attctatatg atatcagtat tactaggtgt tgctgttgga tattctggag
     8941 catggggagc atactacaca gagttgttcc caagtaaata tcgttcgttg tcatctggta
     9001 tgtcatttaa tggaggaagg attatatcta cgtttgcaat acctgctata gctggtacag
     9061 cttctggtac tttaggtatg atgactatct tttacatatc aatcgcagtc tttgtagcag
     9121 gtgctattgt atggttattc ttaccggaga cgttaaataa taaaaaataa aatactagga
     9181 atttattttg ttacttagaa aggggtggct tatgatttca tcaattgatg tatgtattat
     9241 aataggatat ttgctattaa tgttagttat tggttattat tcaggaaaag ataataaaaa
     9301 tcaagaagat tatttcttgg caggtaggtc aatgccatgg attccaattg ctttatcagt
     9361 tgcagctact atgattagtg caaatggctt tataggtggt cctggatggg catatgtaga
     9421 tggaatgtat ccagtaatgg ttaatattac agtaccttta gcaatatttt ttgcactgag
     9481 cattaccacc ccagttatct ataaattaaa aataacctct atatatgaat atatgggact
     9541 tcgtttagga aattattcaa gaggattaac tattattcaa ttttttatta attcattgat
     9601 tcaagctagt tctatggtat atattccagt gcttattatt caaatgatta ctggctggtc
     9661 actttatgct ttagtcccaa tagttgtact tgtatcaatt atatacactc tattaggtgg
     9721 gattaaggca gttatatgga ctgatagtat tcaaatgata gtagtaataa gtgctgtatt
     9781 tatagtaatt attgtagcct taaaaggaat gaatctaagt ttctttgata cattacaaat
     9841 tgcacaacag agtggaaaat taaatacatt tgatttcagt agggatattt cagtgacaaa
     9901 tactttttgg gctactttaa ttggtggaac tgtaatgtgg ataagatact tttgttttga
     9961 ccaatctcaa gtacaaagag ttttaacatc taaatcttta aaatgtgcaa aaaattcttt
    10021 tgtagttagt gcatttgtta tgaatttagt ttactacttt atgctattgg taggagtaat
    10081 tttatttgtt ttttatgggg gaaggacatt tgaaacttca aatgaaataa tgattacttt
    10141 tattttaaat gaacttccag taggagccat tggaattatt attgcaggtg tgtttgcagc
    10201 agcaatgtct agtatcgact ctatattaaa cagtatgaca actgttttta cgaaggatat
    10261 ttatgagttt tatttcaaaa aagataacaa agaatcatca ttaaaagtta caatgattat
    10321 aactgtagtt ataggagttg ttatgacagg ttttattatt atgggatttg gaggaagcat
    10381 caaatcaatt cttgaccttg ttggtaacta tatctcatac tttgctggac cagctacagg
    10441 tgcatttgta ctagcaatgt ttacatataa agctaatgat aaaggagtat ctattggttt
    10501 tattgtaggg ttagttttag gattttttat ttctaataaa tataatgtga gttggttgtg
    10561 gaatccagca ataggtgcta caataacatt gatatttgga tatattttta gtgtcatttt
    10621 aaagaaaaat cataatgcag atgatattaa aaaatacact gcatttggta tgagaaatca
    10681 ccttatttct caaggaatgg ataaagtaga tggagtatct atagtgccat tttctttagg
    10741 taaacaagaa ttaattgtat tagctttttt tattatccaa tatattatat tatttttgat
    10801 acaattttaa gaagaaatac tagatattta aggagaaata ctaaatgaat caaaagaaag
    10861 ttcattataa taaatcggct acctcaaaag atgttgcaag attggcagga gtatctcaat
    10921 catctgtttc aagagcattt ggtagtgcaa atggaaaggg cgtaaaacct gaagtaagag
    10981 aaagaatttt tcgtgtagca aatgaaatag gatatgttcc taatttggta gcacggggaa
    11041 tgattagtgg aaaaactaat gttataggat tgatagttgg tgatagtcta ggtccatttt
    11101 acaatagaat tatcaattta tttgtagaaa aaattcaaga gataggtaaa cagtgtctcg
    11161 tttttaaagt tcctagacaa caaagtatag atagtataat ttcaaaagta attcagtttc
    11221 aagtagaagc tgtgattatt acagcttcgg ccatgaataa ggtgatggct gagacttgtg
    11281 aagaaaacaa tattcctgtg attcttttta atcgttttat acctggtata aaaataagta
    11341 cagtctatgt agacccaatt gaaggtgggg gaatggctgc tgaatatctg cttaagaaag
    11401 gtcataagaa tataggatat attcaattta cgagagaaac aagtgaagaa atggaaaaga
    11461 aaattggatt ttactctaag ttaagacaaa gtgggattca taaccttaaa gaagaatctg
    11521 caaactatga ttatgattct ggatatgaag caggtaagag aatgttagca ttatcaaatc
    11581 ctccaacagc aattttttgc acaagtgact tgatagctat tggtgtaatg gatgctgcaa
    11641 ggtttgagta caagttgagg gtgccagagg atttatcagt gataggatat gatgatattc
    11701 aaatggcttc atggaagagt tataatttaa cgacgattag acagccacta gatttgttga
    11761 ttgaaaaaac aattaacata cttaagtcct tattaaatga agaaaatcca gagtcagtcg
    11821 ttgaaatgtt aaaacctgaa ttgattgaaa gaggttctac actttaagtg gtagatattt
    11881 aattaagtgg tagatattta attaaatggc agatatttaa tcaagtgata gatacctaat
    11941 taagtgatag atatttaatc aagtgataga tacctaatta agttgtagat atctgataag
    12001 aaatttttaa gttttcaatc gttctaaatt gtgagtataa aaatctacta aatttagtta
    12061 tgtaaaatat aaattataag tgtagaaact aggctactag cttttatacc acttcaaaca
    12121 ttactggagg tagttaagtt tacagcagtt tagttttatg tagatataat attctgtaaa
    12181 tagacaaaat taaaatatgt aattaaaata tgtaagtgtc tcaaaatgaa atttttagtt
    12241 tatgaggcac ttttttgtat aaaatcaaat gtatttttat aactttaaca ataaaaagag
    12301 gcttatctct attttgagac agcctcaaat ttttaaaact atattagttt acagcttctt
    12361 ttcctatcaa atcttttaac tcttcatgag tcatagtttc tttttcaaat aagaatttag
    12421 aaacagaatg taaatcttca agattatcct ttaacatctc taaagtctct ttataacaaa
    12481 tttcaactat acgatttgct tccttttgaa ccttatcaca taagaaagtc ttatcatttc
    12541 catcaatagt cataaatcct aagttactca taccatattc acaaaccatt tggttagcta
    12601 tttcagtgac tttctttaag tcgtcctttg caccagtaga tttatgatta aatattaatt
    12661 cttctgaagc ctttccagca agtaatattt ttatttttcc aatcaactca tccttagtat
    12721 aaatatatct atcttcatca ggtaattgaa gaacataacc taatgcttgt cctctaggaa
    12781 ctatagatat tttttgtata ggacaaattc ctactatatc acttacaaga gcatgtccag
    12841 cttcatgata tgctactatt tttttctctt tttcaaccaa tgcagagttt ttagattcta
    12901 gccctgcaat aactctttca agtgccttgt caaaatgttc tgatgtaata cactcactgt
    12961 catctctaac tgcaaatata gctgcttcat ttgctatatt tgaaagatga gccccattga
    13021 aaccatgagt ttttttagct aaaagttcta gatttacaga tttgtctata ggtttgtcat
    13081 ctgtatgtac ctttagaatt tcaaatctag tgtgatagtt tggtgcacct atatgaatgt
    13141 gtctgtcaaa tcttcctggt ctaagtaaag cttcatctaa taagtcaagt ctattggtag
    13201 cacctattat taatacattg gaatctttat taaatccatc catttcaact aaaagttggt
    13261 ttagtgtttg gtctttttcg ttgttacttt caagatgtct ctttgctcct acagcatcta
    13321 tttcgtctat aaatattata cttggagctt cttttctagc tttttcaaat agagttctaa
    13381 ctctttttgc tcccacacct acatattttt caacgaactc tgaaccagta acattgaaaa
    13441 atgatgagtt tgtttcacca gctacagcag atgcaagtag ggttttacct gttcctggag
    13501 gaccataaaa taatactcct ttaggtattt ttgcacccat tttttgatat ttttgtggtg
    13561 acttcataaa atcgactatt tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt
    13621 ctctaaatgt agtctttggt tttgtagaaa gtgaatcatc tgtttgatta tctctttcca
    13681 tttttgtctg tgcaaagaca ggagctggat tgttgatttt cttaaaaaag ttgtttagca
    13741 ttttcatatt cacacctcag gtaattaatt attgttaatc aattttatat tttccatatt
    13801 tactattttt tattatttat ttttcatgga aaaaatatac atgttatcta aataaaaata
    13861 aatttgtaag atgtatgaaa aaatgaattt atgtata
//
LOCUS       DAELNY010000097        13884 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.097, whole genome shotgun sequence.
ACCESSION   DAELNY010000097 DAELNY010000000
VERSION     DAELNY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.097"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            200..535
                     /locus_tag="KPE55_002337"
     CDS             200..535
                     /locus_tag="KPE55_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493397.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            672..1949
                     /locus_tag="KPE55_002338"
     CDS             672..1949
                     /locus_tag="KPE55_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG0493398.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2070..3458
                     /locus_tag="KPE55_002339"
     CDS             2070..3458
                     /locus_tag="KPE55_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0493399.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRKTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3829..4542
                     /locus_tag="KPE55_002340"
     CDS             3829..4542
                     /locus_tag="KPE55_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG0493400.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4660..5529
                     /locus_tag="KPE55_002341"
     CDS             4660..5529
                     /locus_tag="KPE55_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0493401.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5684..6829)
                     /locus_tag="KPE55_002342"
     CDS             complement(5684..6829)
                     /locus_tag="KPE55_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0493402.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7162..7938)
                     /locus_tag="KPE55_002343"
     CDS             complement(7162..7938)
                     /locus_tag="KPE55_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0493403.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8520..9494
                     /locus_tag="KPE55_002344"
     CDS             8520..9494
                     /locus_tag="KPE55_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0493404.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9592..9897
                     /locus_tag="KPE55_002345"
     CDS             9592..9897
                     /locus_tag="KPE55_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0493405.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            10053..11213
                     /locus_tag="KPE55_002346"
     CDS             10053..11213
                     /locus_tag="KPE55_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0493406.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11263..12165
                     /locus_tag="KPE55_002347"
     CDS             11263..12165
                     /locus_tag="KPE55_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0493407.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12245..13138
                     /locus_tag="KPE55_002348"
     CDS             12245..13138
                     /locus_tag="KPE55_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG0493408.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
BASE COUNT         5392 a         1526 c         2382 g         4584 t
ORIGIN      
        1 tgtagttaca aaaaagttac caattgttat gtatttacaa aactgatata ttaattacat
       61 aaaatctaaa atagtttgta ataggagtat atataataaa aattttaatt aaataaagct
      121 aggtatattt ataatattaa taaatatatt gctataaaag tttgtttaat aaattaaata
      181 aagaaaagag gagaataaaa tgtacaacat agatgatatg atatataatt gtccagtaga
      241 agctttatca aatattttag gtaaaaaatg ggtggctata attatatgga aaatacaaga
      301 agataaaaaa cgatttggag agctacaaag agctatacca gactgtagta agaaaatgtt
      361 agttcaacaa ctagattttt taattgaaca ggggattata gtcaaccaaa aaaagttggt
      421 taataatgtt gtagagtcaa catatttttt aagtgaatca ggattacgat tgcttcctgt
      481 catggaaaaa atgattatgt ggagcaataa aaatttaaat tgtgataaat actaaaattt
      541 actctgtaag taatgaaaat ttaattaagc tatgagtaga aaaatgtatt aaaattacat
      601 aattttttag ttagatttta atttagaaat gggattgtaa tattggaaat aaaataagga
      661 ggaattttaa tttgcaaaat gtaaatttat ttttagtatt tatagaaggt atagtatcat
      721 tcttttcacc atgtatactg ccaattttgc ctatttattt gagtatattg tcaaatagta
      781 gtgtagaaaa tttaaaagaa ggaaagacga gttttatagg aagttcttta tttaaaaata
      841 ctatattttt tgctcttgga atctcaacaa cattctttat attgggttca tcagtaaaag
      901 tattgagtat gttttttaat gaaaataaag atttaataat gtttattggt gggattataa
      961 ttattatcat gggattattt tacatgggaa taattaaatc ttcaatattg aatagagaaa
     1021 aaagatttaa tgttaagttt aaagaaatga aagcaataac agcttttata ttaggtttta
     1081 cgtttagttt tggatggaca ccttgtattg gtccaatatt ggcatctgta ttagtcatgg
     1141 tttcaagttc aagtaatcat cttagtgcca atcttttaat agcagtatat actattggtt
     1201 ttattttgcc attcataatt acagctatgt tttacagtaa actatttaaa actatagata
     1261 agataaaatc taatatggaa attattaaaa aaataggtgg gattatactt atagtatcag
     1321 gaatattaat gatggtaaat ggatttggaa gtataagcaa acactttaat acgtcacaaa
     1381 atagtaaaat tgaaagcaag caagaagaaa ataagcgaga aaattctact gataaagaag
     1441 aaaattctga tggtaatgat agtcaaaaag actctaataa tgacaataat gataaagggt
     1501 ctaatgatga agatagaata aagtctattg attttacttt aacagaccag tatggtaaaa
     1561 cacataaatt aagtgattat gaaggcaaag ttgtattctt aaatttctgg gctacatggt
     1621 gtcctccatg taaagaagaa atgccttata tagaacaact gtataaagac tataataaaa
     1681 ataatgatga tgtagtaatt ttaggagtag cttcacctaa tttaggtaga gaaggttcaa
     1741 gggaacatgt agttaacttc ttaaaagaac aaggttatac attccctgtt gtacttgatg
     1801 aagatggagc attagcatat caatatggaa ttaatgcttt tccaactaca tttataattg
     1861 acaaggaagg atatgtaact cagtatattc caggagctat ggataaggca actatggcat
     1921 catttattga aaatcagaga aataaataaa agtatttaag tataagttta taaatcaatt
     1981 taaggttaca aaaaagtgcc taattgtacc acctaaataa tcttgttata gtaaaagtat
     2041 aaacaataca agaaagggtt gaattttata tgaaaaaaac atttgatgct ataataatag
     2101 gatttggaaa aggtggaaaa acattagcag gggatttggc aaacagggga ttaaaagtag
     2161 ctttaataga aaagtcaaac aagatgtatg gtggaacatg tgtaaatgtg gcatgtatac
     2221 caacaaaaag cttagaaaat tcagcaaaca gcgtaaaaac aaagaatata aattcttggg
     2281 atgaagtaca agctgaatat gagaaagcta tagataaaaa agaaacttta attacaaaac
     2341 tcagagaagc taactataat aaattaaatt caaatgagaa tgtaactata ttcacaggaa
     2401 tgggaacttt cattgatgaa aaaacagttc aagtcaaaac tgaaaatgaa atttatgagt
     2461 tagtagcaga taatatattt attaatactg gttcaagacc atttatacct aatattaagg
     2521 gaattgaaaa taagaatata gtatatgata gtgagtctct tatgaatcta agagttttac
     2581 ctaaaaaaat gacaattata ggagctggat ttataggttt agaatttgct ggtatttata
     2641 gttcatttgg agcagaagtt acaatattaa actctaataa tggcatttta ccaaatgaag
     2701 atgttgagga ttcagaagaa ataataaaat tacttgcaaa gagaaatgta aaaattgtta
     2761 ataatgcaaa tataaaagag attaaagaag tttctgaatt agcaatagta gaatatgaag
     2821 tagatggtaa atctaaagaa ttaacaagta atatgatact tgtagctaca ggaagaaaag
     2881 caaacactga agggctaggg ctagaaaatg caggtataga attaaatgaa agaggattta
     2941 ttaaagtctc agaaactctt aagactaata aagagcatat atgggctata ggagatataa
     3001 atggaggtcc acaatttact tatatttcat tggatgatta tcgcatagtt attaatcagt
     3061 tatttggaga taagactaga aaaactaatg atagaaagaa tatcccaaat tctatattta
     3121 taagtccagc attttcaaga gttggtctta atgttaagca agctaaagaa aagggatatg
     3181 aagtattagt ggcaaagatg ccagtagaag caattccaag agctaaacag attggcaagg
     3241 cagatgggtt tataaaaata gtgatagata aaaaatccaa taagatactt ggagctagta
     3301 tgatatgtga aaattcaagt gagattatcc atcttattca attagctgta gatttagaag
     3361 tagagtatac atatttaaga gaccgtgttt atgcacatcc aactatgaca gaagctctta
     3421 atgatattct atctcctaat atgataaaag aagtataaca taagtttaat atataaacct
     3481 aaaattatca ttataagtag attttagaaa gagactatct caaaaaatag agataagtct
     3541 cttttttact gtttaaataa taaaaatata tttgatttta taaaagaaga ttattttatg
     3601 agctaaaaag tttatttaga aacactacct atttatgtta taaagttata aatataatat
     3661 agaatctatt attctgtatt attaagtggg gttaagcgtt ataattaatt ataaatattg
     3721 aataatctaa atggataaga taatggtatt aaaaattatc ttaaaaggtg aaataatctg
     3781 tatttaatgt agtacagtta tatctttaaa gcaaggtggt gaaatgatat aaataagata
     3841 tctaaaagag ggtttgatga tagtgataaa agatggtttt caagtaaaga attaacaaga
     3901 cttataaagg ctaaagaaga aatagaatgg ttattaaata gggattacaa aatagaccca
     3961 gtcgttactt ttgttggtga tagatatcag ttttctataa gacaaagaga tgcattaaaa
     4021 agagcagtct gtactgatga aaaaaatatt ataagacaat caaagaggct ttctctagat
     4081 aaaatcaaag aagataccgt aaatatagat ggctttaatt tgataatatc catagaggta
     4141 ggattatctg gtggtactct agtaatagga agtgatggca atataagaga tttagcaggt
     4201 cttaggggaa cctataagat tatagataaa acagaagaag ctttgaatct gataggtaaa
     4261 ttttttaata aatacaatgc acagaatctt aaattttatt tagatgctcc agtttctaat
     4321 tcaggaaatt taaagtatag gatattagag catgccaaaa cttggggaat tgaaactgaa
     4381 gtagaattag taaaaaatgc agatgttgta ttggaaaaat tagatagagt agttagtagt
     4441 gatgcagtca tagttgacaa gtgtataagt tattttaatg tggctagagg tataattgaa
     4501 gagtatatta aagaatgtaa tatcataaat ttaaataaat agataaatat aggtgagaat
     4561 atataagtat atgtagcaat attcttagta atacaagtaa agtttgaaaa taggagaacg
     4621 aatacatatc gaatttcata aaatgttgta aaaagacata tgagaggatg gatatgtgtg
     4681 aaattaacta aactaaatat taaaaaatat agagcaccta tatataaata tgctttagca
     4741 aatgtaaatt tgaggtctgc taaatctact aattcaagta tcataacagt aatacctcag
     4801 ggagccaaaa tggaagtatt ggatgaagaa gatgactgga ttaaggtgat gtacaattct
     4861 caagaaggat atgtgtataa ggatttggta tcagtaagcg aatatgcttg gagtaactta
     4921 aacttaagag aagataaatc tacaacttcc aatataatta cagtaattcc tgaaaaatct
     4981 agggttgaag tccttcaagt agatggagat tggagtaagg ttgtatatga tgataaaata
     5041 ggatatgttt ttaattattt tttatcaatt gatgggaata aaccaaatga attagattat
     5101 aagtattttt atactgacat gattaaattt gttaacgaaa ataatattaa aagtactagc
     5161 gattatctaa ttgtaacaga ccttagaaat aaatatactt atatttttaa gaaagataat
     5221 ggaggttggg gacaacttta caagtggcaa tgtactatag gcaagcctga gactccaact
     5281 ataacaggta tattttatat aagtggaaga aaaccttctt ttggaacgga tgagtattca
     5341 gttaagtatg ctactagaat taaaggtgga tattactatc attctgtact atatgattca
     5401 acaggaagtt atataataga tggcagactt ggtgaagctc ttagtcatgg atgtataaga
     5461 ttaagtacgg aaaatgcaaa gtggatatat gataatatac cagatacaac aacagttata
     5521 attcattaag aaaaattttg agttttttat tgggataatg attcttaaat taataggatt
     5581 atatattaac ttctcataaa gagtactatt taaaaagtgt aataagagag tgctattaac
     5641 aaaacaaaga tattttgagg cactctctta taaatataca taattaagat attttcataa
     5701 acaattctat aatcttatct ttatcaaaga aaacaggatt agcaccagca caagcatctt
     5761 tcatagagtt ttctgcaagc aattcaaaat taggattatc aacacctaca tcttttaaag
     5821 attttgggat tccaacctct tcagaaagag cttttatctt atctattaca aagtcaacac
     5881 actctttatc tgatttatta tttacatcca ttccaataac ttcagctatt ggtcggaatt
     5941 tggcaggggc tgatttagca ttttcctcct caactattgg aagtagcatg gcattacata
     6001 ctccatgagg taaatcatat aaacctccta attgatgagc catagcatgt acatttccaa
     6061 gacctgcatt actaaaggca attccattta aaaaacaagc ataacacatt tgttctctag
     6121 cttctatgtc attaccattt ttaacagctc ttggaaggta tttaaatatc tgctttatag
     6181 catataaggc agttgaatct gtaacatcat aagcaccatt agctactaca gcttctatag
     6241 catgagttag tgcatccata ccagttgctg ctgttaaatt agatggtttg ctaatcatta
     6301 attctgggtc atttactgtc attgtggcta gtgaattggt atcaaccatt atcatcttta
     6361 catgtctgtc ttcatcagtt ataacatagt ttattgtaac ttcagcagat gtaccagcag
     6421 tagtagttat agctatgata ggaagacatt ttttagaagt tttgttgata ccttcatagt
     6481 ctctaatatc tcctccatta gtggctaata tagagatagc tttaccacag tcttgtggag
     6541 aaccacctcc tatagttata acgaagtcgc aattttctga ttttaatatt ttaagtcctt
     6601 tattcacatt agttacagtt ggattttgtt taacttcatc ataaacaaca aagtctatat
     6661 ctatctgaga taaaatatct gttacttttt ttacagtacc atttgaaact agaaatttat
     6721 cactaacaat aaatgctttt ttagttccta atgatttaat tgaatcttta gcttcattaa
     6781 gacatccttt cccaagtaaa tttattggtg gtacataata aatgctcata actaaaatct
     6841 ccttttattt tcattgattt ttatgtaaaa atatatatct aacaattttc actaatttac
     6901 tcataatata tcgtatctta gattgagata gggttgctgg ttcttatata agaacttaat
     6961 tcgtaattac gattatgatt agaatttaac atagtatttt tatcatgtca ataaaaatat
     7021 aaaaaatatt ataaaaaaaa caaagttact gtagttaaat cagcaacttt gtttttatag
     7081 tataaatcaa ataaatacat tagtttaaaa caaaattttt aattattatg ttttgatatc
     7141 ttccatatac agaatattaa tctaatttat tacaatgtct tcattacgtt cttccatcat
     7201 tgattgaata tttttctgag catttaagag taattttttt atcagttcta ttttttcgtc
     7261 attagctcta aataaaggca gtgttacact aacagcagct cttatacatc cattttttct
     7321 tattggtacg gcaagacatt ttaggtgttc cattgattcc tcacactctg aagctatttg
     7381 tgttctttga acttcaagaa gctggtcgta taaaacatca aaatctgtaa ttgatttttc
     7441 tgtaaaacct tttagaccat ctggatataa gcttattagt ttttccttgt caaaatcaca
     7501 taaaagagct tttccaagac ctgtacaatt agcaggtatt ttttttccaa cataagaaat
     7561 aagtctaatc ggttctggag aatctacttt tccaatataa agaacttcat tattgtcaag
     7621 tataccaagc tgacaagttt cagaacagga attaactata ttagtcattt cgtcaattat
     7681 atagttaaaa atagttcttt tttcaaaaaa tgaagaccct aatgtgtaag aacgtatccc
     7741 tatagaatat ttggatgtgt ttttatcaat agtaatgtat tttctagcat taagtgtatg
     7801 aactatagga aagatgctac tttttggagc attgataaca tctgctattt ctgtaagagt
     7861 atatccattt tcggattctg ctaatgcttc taaaatatct aatatcctag ctgttgggcg
     7921 atgattatct gaagtcataa atttccccct ctttttatta atttaaagta ttagaatatg
     7981 ttgattttag cacactatac gatattttac aatgtagaaa ataaatatgt aaatttatat
     8041 ataaataagc tcttgattta gatacagata gatatttttt tagactgtac attaaggaat
     8101 aactaatttc caaaatatac ctaaagaaag taagtgatat ttagttatat aatttaagta
     8161 aatatatgca atagttattt ttatatgtaa atatcaaaat attatgaact tatattttat
     8221 ctatttttta cactgtataa aaactttata gttttgaata ctatataatt ttaaatacgc
     8281 tgtatttttg catataagga agtcatttga taatatacaa ggtattatca gaaagtttga
     8341 aaaaattttg acaaaaattt atttttttta ttgactcata aataggaaaa tgctatcatt
     8401 aattcaaaga ttcgtaatta aaaacttagt tcttaaatac gaaccgaaac ggccgttaga
     8461 attaaagcta aaaacttaat aagagtaatt aaatatcatg ttttaggagg attaacttta
     8521 tgataatgga ttttattaaa aaaattcctg caggtatgat gatagtgcca atgtttatag
     8581 cagctttcat taataccttt tgcccagata ttgtacaaat aggttcgttt acgacagcag
     8641 tattttcaag tgttgggtct gcaactataa ttggggttca actggtatgc ttgggaactc
     8701 agcttcaatt tagagaaatg ccaaaagttt taaagagagg tggtatactt cttggttcta
     8761 aatttgctat aggtgctttg ataggtatac ttataggaaa gctatttgga atggatggtg
     8821 tccttggtct gacaacattg gctgttataa gtgcagttac aaatagtaat ggtagtatat
     8881 atctttcatt gatgacttca tatggagatg aaacagattg tgcaactatg agtttactta
     8941 ctttaaatga tggaccattt tttacattaa tagctcttgg gacttcaggt cttgcaaatg
     9001 taccattgtt gtcattattg gctgcaatag tgccaatact tgttggtatg ataataggaa
     9061 attttgacaa aaaaatgaaa gagtttttag agccagggtg taatttactc gtaccttttg
     9121 tagggtttgc acttggtagt agcataaatc ttgcaagtat actaaaagga ggtttatcag
     9181 gagtattact tggattaata tctgtatttg taggaggtat atttatagta ttctgtgata
     9241 aatttatagg aaaacgtcca ggatatgcag gatgggctgt agctacaact gctggaaatg
     9301 caattgctgt acctgctgct gttgcattag ttgacccatc atggcaacca tatgttgcaa
     9361 cagctacaac acaagtggca gccgcagtag ttgtcactgc aatattagta cctcttataa
     9421 ctagttggtg ggctaagaag tatggatgtc ctaagttccc aaaacaaaat gaggatggta
     9481 caataacagc ttaatttata ttacagtgag ttgttggtaa aataataaat tctttaatta
     9541 gattttagga tattaataaa attatattat ttataaagga gagcaagaat aatgataaat
     9601 gcagtgataa ttgatgaaaa agataatgtt gcagtagcta ttgaagcaat agctaaaggt
     9661 agtgaaatta gttttaaatt aaaagacaaa acagtaaaaa cgattacagc actagatgat
     9721 ataactattt atcataaatt ggctataaaa gatatggcca aaggtgataa agttactaaa
     9781 tatggtgaac atataggaga agcaaatcag gaaataaaaa gtggccaaca tgtacatatt
     9841 cataatgtta gaagtgttag agaggatttg gataaacaag ttatgactaa tatataaaat
     9901 aatatttata gagttataaa agttgtattc attgctttta gtgtcaaata gtttttaaat
     9961 aggtttttag ctgtttgatt agtgagtgta ataaaaatta taatatataa aaattataga
    10021 aagtatatag actattttag gaggaaatca gtatgaaatt ttatggatat aaaagaccag
    10081 atggaagagt tggtataaga aaccacatat taatattgcc tacgagtgtg tgtgcatcag
    10141 atacaactag aataatagca tcacaagttc aaggggcagt tacctttaat aatcagaatg
    10201 gatgttcaca agtacattca gaccaacaat taactatgga tgttatggct ggttatgcag
    10261 caaatccaaa tgtatatggt gtaatagttg tatctttggg atgcgaaaat tgccaaaatg
    10321 atttggtagt tgatgcaata aaagagagaa caaataagcc aataaaaact cttgtaatac
    10381 aagaagaaca tggaacttta aaaactatag aaaaagctgt tcgttatgct agagaaatgg
    10441 cacaagaagc ttctttgctt agaaaagaag aatttccaat atctgaactt atactaggaa
    10501 ctgaatgtgg tgggtctgac ccaacaagtg gattagcagc aaatccatta ataggtgaat
    10561 taagtgataa acttgtcgat ttaggagcaa catcaatttt aagtgaaact actgaattta
    10621 taggtgcaga gcatatacta gctaggagag cagtaaatga agaagtaaaa gaaaaaatat
    10681 tacatatagt acatagatat gaaaattcat taaaattggt tggagaagaa gttagagaag
    10741 gtaatccatc tcctggaaat atagctggtg gtttaacaag tttagaagaa aaatcacttg
    10801 gttgtataca taaaggtggt catagacaaa taagtgaagt atatgattat gccaaacaaa
    10861 ttgataagaa aggtcttgtt ataatggata cccctggaaa tgatgcttca tctgttgctg
    10921 gtatggtagc aggaggagca caaatagtgg tatttagtac aggaagagga acaccatcag
    10981 gaaatccaat agcaccagta ataaaaataa ctggaaataa aataactttt gcaaatatgg
    11041 aagataatat ggattttgat gctagtccag ttatctatgg tccacaaaca atggaagaat
    11101 taacagatga tttgttaaat atggttgtag atgtagcaaa tggaaaacaa tcaaaagcag
    11161 agtcccttgg atatactgaa atggcaatag ctagagtatg caactatgta taatctttaa
    11221 gtaagaaaat aaatacatag aattttaata aagggtgaga ttatgaagtt tgtaagtttt
    11281 actgaaggag aaaatgaatg taaaagaact ggagtttttt ctaaagatga attatttatt
    11341 atagatgtta actctttaaa tctcagtaga aattttaagg atttaaatga attaattcaa
    11401 aatgtatcta gtgaagatat tattaagttg aaaactatta taaatgaaag tacattctat
    11461 aattatgatg tttttaaaag agagagatgt acggtacatg ttcctataga aaaaccaata
    11521 catgatatct tatgtgtggg agtgaactat aaagaccatc ttgaagaaac tcaaactcac
    11581 tttgatgata gttttgaaga accacaaaaa acggtttatt ttacaaaacg tgtgacaaag
    11641 gcaataggtc cagaagatga gatagatggt cattttgaga taaataatca actagactat
    11701 gaagttgaac ttggagtagt aataggtaaa acaggtgtta atattagaaa agaagaggta
    11761 gaagattata tctttggtta cactgttata aatgatattt ctgctagaaa gttgcaaaaa
    11821 gaacatgttc aatggtttag aggaaaagga ttagatacat ttacctcttt aggtccatgt
    11881 attttacata aaagtgcagt tccttttcct ataaaactta aagtatgtag tagggtaaat
    11941 ggtgaagaaa gacaaagttc taatacagga ttattcatat cagacgtttc agacatagtt
    12001 tcagaacttt caaaaggtat gaccttagag gctggagaca ttatagctac tggaacacct
    12061 tctggagtag ggatgggatt ttcacctcct cgatatatga gtagtggaga tgtagttgaa
    12121 tgtgagattg agagtattgg aattttaaaa aattacatta agtaactgtt ttaatataat
    12181 taatattaaa gctaaatctg atgtattaat gaacatatta aaataaattt taggagttta
    12241 aattatgaaa gcagaatttt tgacaccagt tgttacaata tttgatgaag aaggaaattt
    12301 agataaagaa ggtaataaaa aagtgtatga ccatcttata gatggaggtg tagatggact
    12361 agtcataatg ggaagtactg gagagttctt taatatgtct atggatatgc aaaaagaact
    12421 tatagattta gtaactagtc atgtaaataa gagagtaaaa gtgtttatag gtacaagtcg
    12481 tatgtgtata aatgaatcta tagaactttc aaactatgca cataatagtg gagcagatgg
    12541 tgttatgata ataagccctt attatttctc tcttgatgat gatagtattg agttatttta
    12601 tagtgaaata gctaagaata cagaggctga aatatattta tacaattatc cagatagaac
    12661 tggttatgat ttaagtccag aacttacatt aagacttgca agaaaacata aaaacatagt
    12721 tggatataag gatacagtaa ctttgatggg acataccaga gatttaataa atactatgaa
    12781 aatagagttc ccagaattta aggtttatag tggttatgat gaaaacttta tacataatct
    12841 aatgtctggg ggagcaggtt gtataggggg gttatcaaat ttaattccag aagtttgctc
    12901 caaatgggct gaagcttaca ataatagaga tatggagaag gttatagaaa ttcaaaaata
    12961 tattaataaa gcaatggatt tttacttaat aaccaatcca tttataccag caatgaaaaa
    13021 agctatgaat atcagagggt tacaaataag tgattatatg actgctccat ttgtaaaacc
    13081 aaatgaaaaa caagtatgtg aaattaaaaa attgatgaat gaattaaata tatgttaact
    13141 tactaagtaa gatatatgag caaaaaaaga gtgtttaaac tgatttttat aatcatttta
    13201 aacactcttt ttatatatgt taaatcaact ttgaaaagtg gtcaacaata aattacaatt
    13261 atgttaataa aaattaaata atactaagat agcagattag tttaaaagtt agcacaaata
    13321 ttaaagcaat agcctttatc tattaaaaat aatcttggag ttttacaaac atttttagat
    13381 aagtataagt taacaataag agccacatat aaactactac aaatcatgta ataattaatc
    13441 taataaatga taaacactta gacttcaagc ttaaaagtat aaagataaat gaatttacat
    13501 atcaagattg gcaataattt tgtgatacaa agcactatag taagaatgat ttaataccaa
    13561 tgattttgaa agaacatata gaaaaataca attagaataa aataaatttt agagggaagg
    13621 cattgccttc cattttttgt gtttttctag aattgggtaa attttacgat tctagtaaag
    13681 cacaaaagaa aatctgttgg tcttgaacat tttatattag taattatagg tagcaatata
    13741 caattactac aaaagtgaaa tattaatgaa atgtaaatca atttaaaaaa attaaattaa
    13801 aaattacaat agatatataa gaaatttaaa ggagaaataa ggcatgaaaa atgtattaag
    13861 tgttgaaaaa tggaaaagta ttat
//
LOCUS       DAELNY010000098        13875 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.098, whole genome shotgun sequence.
ACCESSION   DAELNY010000098 DAELNY010000000
VERSION     DAELNY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.098"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..3048
                     /locus_tag="KPE55_002349"
     CDS             145..3048
                     /locus_tag="KPE55_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0493409.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3081..3527
                     /locus_tag="KPE55_002350"
     CDS             3081..3527
                     /locus_tag="KPE55_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0493410.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3650..3952
                     /locus_tag="KPE55_002351"
     CDS             3650..3952
                     /locus_tag="KPE55_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493411.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4133..5539
                     /locus_tag="KPE55_002352"
     CDS             4133..5539
                     /locus_tag="KPE55_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0493412.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKMKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5883..6941
                     /locus_tag="KPE55_002353"
     CDS             5883..6941
                     /locus_tag="KPE55_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0493413.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7040..7726
                     /gene="araD"
                     /locus_tag="KPE55_002354"
     CDS             7040..7726
                     /gene="araD"
                     /locus_tag="KPE55_002354"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG0493414.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEIRNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7744..8391
                     /locus_tag="KPE55_002355"
     CDS             7744..8391
                     /locus_tag="KPE55_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0493415.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8564..9931)
                     /locus_tag="KPE55_002356"
     CDS             complement(8564..9931)
                     /locus_tag="KPE55_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG0493416.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10121..10209)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10637..12757
                     /locus_tag="KPE55_002357"
     CDS             10637..12757
                     /locus_tag="KPE55_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG0493417.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12861..>13875
                     /locus_tag="KPE55_002358"
     CDS             12861..>13875
                     /locus_tag="KPE55_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG0493418.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNK"
BASE COUNT         5523 a         1575 c         2449 g         4328 t
ORIGIN      
        1 taaatataca aaatgtaaat acttatcaaa atgtataaat acttatgaca tctagttact
       61 tacagggttt aagtagaaaa aaatatcaaa gtaagtatgg catgatagtt gcttatatat
      121 ttatcaagaa aggagttatt aagcatggag ttgagagata aacttgtaaa catcataaat
      181 acagaaaata aaaaagatcc attaacagat gttcaaatag caaagttttt aagtacaact
      241 agagagaaca taactaattt gagaaaagaa cttaatataa gtaattcaag gcaaagaaga
      301 tatccttatt taaaatcagc aatttcggca atattacaaa agaataagaa tatttctata
      361 tcagaaatta caagggaatt gatgacagaa ggctttaata tatcgagaag agttgtggag
      421 gaacttttac ctaaagaaag tttagaaatg gatgtagtag aggaagatga agaagaatcg
      481 aatgacccat ttgaaactct gataggtaat aaagggagtc ttagaaatgc tgtagagcag
      541 gctaaatctg ctatattgta tccaccaaaa gggttaccaa cgttgattat tggggaaagt
      601 ggagttggta agagtttatt ttctagacat atgtatgagt ttgcaaagca aaaaaagata
      661 gtaaaagaga gctccaattt tgttgtattt aactgtgctg attatagtga caatccacaa
      721 ttattattat ctttgttatt tggatataaa aaaggtgcat ttacaggtgc tgaatatgat
      781 acaccaggtc ttgtagaaga agctgatgaa ggagttttat ttttggatga aatacataga
      841 ttgcctccaa aaggtcaaga aatactattt tctatcttag ataggggaaa gtttaggaga
      901 ttaggtgaaa ctaatgctga aagaaaagta agtattatgt tgattggggc aactactgaa
      961 aatgtagaga ctaatttatt attaacattc agaagaagga taccaatgct aataactttg
     1021 ccaccattac atgatagatt actaaaagag aaaatagacc ttatatacaa tatctttcaa
     1081 caagagtgta atagaataaa tgcaaaaata tttgtagata aaaatgtgat tgaaatactt
     1141 gcactaaaaa agtttagtgg aaatatagga caattacaaa atatgattca ggttctttgt
     1201 gcaagagctt ttatgaagtt tataaattcc aaagatgaag atagtgaatc tatagtagta
     1261 gtagatataa atgaagtttt aaagcttaaa gattcgttta aagatgctgc ctttcaagag
     1321 attgaatata cagagataag aaaatatttg aaaaatgcta tatttatacc atttaattta
     1381 gaagaatcat cagtaaaagg aagtctttta agtgatgaat atatattacc agaggacata
     1441 tacaaaacaa ttgaaaagaa atattatgac ctaaaatccc tagacattga agatatagat
     1501 atagagaata ttttatggac ttttatttta aatagatttg ctgacttaaa gtttaatctt
     1561 gatagtaatg aagaaatttt ttcaatgagt gatttaaata gttttgtaag tgatagttta
     1621 gttaaactgg taaaagaact tatgaatgat atagttgaaa aaaatatgaa tttagaagta
     1681 aataaaaata tgttcaaata tctagcaata cacttagaag aagctataaa aagaattaag
     1741 ctaaatcaaa agataataaa tgtaaattta gaaaaaataa agattgactt ttcaaaagaa
     1801 tatgaaatta gtaaaagttt tgcaataaaa attgaggaat caattggtat taagatttca
     1861 gatgatgaaa ttggatttat aacgctctac ataaagtctg ctctaaaaaa tgaagtgaaa
     1921 aaagataaag tagggttaat aataatttct catggacgta tagctacaga gactgtaaat
     1981 gtagttaaag aacttttagg tgtaaagttt cctgtggcta tagatatgcc tttagatgaa
     2041 aaaccaataa atatatacaa taaggcagta gaattgtcaa aaataataga ccaagggaaa
     2101 ggcatattgt ttttggtgga tattggttca cttacaaaca taggtcaaat agtaaataag
     2161 agaaccaata taagtactaa aactattgat agagtagacc tcctaatggc actagaagca
     2221 actagaaaag tttctatggg agaggaagaa cttgatgaaa tatttttttc tttgctaaaa
     2281 gataggatgg gatataacta tgaattagaa aaacacatag ataaatcaaa tgcaattata
     2341 acattatgtc ttactggaga aggaactgct aagtatataa gtaaaacgtt ggaagaaaag
     2401 tatgataaca ctaaatgtta tcaaatgagt gcattggatg aaagtttatt ttgtaaaatt
     2461 gaaaatctaa aagagacaaa caatatatta gctattgttg ggacaataaa ccctaagata
     2521 ccaggtataa attttatacc ctataataaa gaacttttca aaaatttgga tatctatcta
     2581 tcaaaatcaa aacaacaaga tgacagacca attaaatcgt atgaaagaat gcttgatgag
     2641 gatttagtga tttttgaacc agatatatat tttaaaaagg atttattaga atatgtatgt
     2701 tcaattctta taaataaaga atatgtagaa aaagattact tagattctgt tttacataga
     2761 gaagaaatgc ttccaacata ttctaaagga gctataggtg tgcctcatgg agattcttct
     2821 actgtaaata gtacaaggtt tgtttttgtt aaactaaaga atcctattga ttggggtgta
     2881 ggtaatgtaa atttcatatt tatgccagtt tttcaggcta atgataaaga gattgttaaa
     2941 aatgttcttg agatactaaa agatgcagaa tttatgaaca atgccaataa gtgttttgat
     3001 aaagatgctt ttagaaggat tatatttgat aaatttaaac atctgtagcc atagcaaaaa
     3061 aattgagagg agttagagta atgaatgtag atttaatatc attgggttta gaatctaatg
     3121 attatgaaag tgttattgaa gaattaggaa gtataatgtg taaaaaagaa tatgtaaaag
     3181 aaacatacat aaatgctgtc ttggaaagag aaaggacatt accaacaggt ctagatattg
     3241 gagacatgtg tgttgctatt ccacatacag attcaaagca tgtaaatgag tctaatgtag
     3301 ctgtagggat attgaaaaat cctgtaaagt ttaattcaat gatagaccca aaagatagac
     3361 ttgatgtaga acttgtattt ttattagcag taaagaaccc agactcacaa gtaaaactgt
     3421 taaaagattt aatgtcagtt tttcaaaata taaagttatt aaaaaatata aaaaacgcat
     3481 caacaaaaga agaagtagct aaattattgg attttataga aatttaatgt aggagaattt
     3541 tataaataaa aaattaaaat ttaaacaatc aaagtaagta ttattttttt aataggttta
     3601 atcttatata aaataaaaat ttagagtgaa aaattaggag gaaattatta tgaaacaatt
     3661 taatgtatta tcagtttgtg gttcagggac agttacatca tctatggtag caggtaaatt
     3721 aaaagaagta ctaggtaata aggggatttc tattactaca acagaagcta gaccaacaga
     3781 agcactaaat ttagcacaat caggaagatt tgactttata acatttacga gtccacttca
     3841 aacaggcgat tatggaatac caactataaa tgcatttgct tgcctaactg gtatagggga
     3901 agaggcattc tttgaagaag ttttgactgt tattgaaggg atagataagt aagttttgga
     3961 gacgtgaatt tatagtattt aaatcaatga tttagatact ataataagct aatattaact
     4021 aaatatttgt ggaaaaaaca ttttatatgt tgtcataaat taaaaaatta taaaaatagt
     4081 cacttaagta ttaaattaag cttataaata tttataagta ggagggaaaa agatgttaga
     4141 atttatgaaa tctataatcg atacatttgg ttctgcaatt atagttccaa ttataatatt
     4201 cataatcgca aagatattta aagtaactac taagaaatcg tttctttctg ctgtgtatgc
     4261 aggagttgct ttacaaggtt ttacactgat tttaaattca tttacaccag taataacacc
     4321 agttatcaat aggatggttg agagtacagg agtaaaccta ccagtttttg atgttggttg
     4381 gcaagcaaca tcattggttg cattttcaac aagtgcaggt atgatatatt taggtcttgg
     4441 tatattatta caaacaattt tatttttgat aaaatggaca gatgtgtttc aaccatcaga
     4501 tttatggaat aactattctt atatggtatg gggagcaatg gttataggag taacaggaaa
     4561 ttttccttta ggtatagcat gtatggtttt attaaaccta tatagtttat taatatcaga
     4621 gttagttgca aagagatggt ctagttacta tagatatcct aattgtacaa taatagctat
     4681 gcataatgtt gaagcttctg tgtttgcagt atttgcagac cctatataca ataaattggg
     4741 attaaataaa atgaaattaa atccaaaaga attagagaag aaattagggt ttttaggtga
     4801 accaattact ttaggtcttt ttttaggtat gtttataggt atattaggta atatgacgag
     4861 aataaatact atggaagctt ggggagaaat aatgaaagta ggtatatcta caagtgcagt
     4921 gatggcaatt ttccctaaag tagcaagtat gtttgctcaa gcatttgcac ctataacaga
     4981 agcagctaga aagattatgc aaaaagctgg aaaccgtgag tggtacatag cagtaaatga
     5041 tgctgttgga tatggtgaac ctgcaacttt aatatcaggt cttatattaa ttccaataat
     5101 gcttgtaata gctatggtac ttcctggtaa taaggtatta ccagttgtgg atttattggc
     5161 aattccatat atggttcaag gtttagttgc catacataat ggtaatatac caaaagtatt
     5221 agtttcaggt ataatttggt ttggattagg attatatgtt tgtacatcta cagcaccatt
     5281 atttacagat atggctacaa atataggtgt agctatacct gcaggagcaa tgttaataac
     5341 aagttttaat atactaggaa aacctttgat gggattagtt ttctttgcat ttctaagtgc
     5401 aaatccaatt tatataggtc tttcagttgt aatttacttt gttctatggg cattgtttag
     5461 aaagaacaaa acaagtattc ttgattattt agaaaaacaa gcacttaaaa atgtagaaga
     5521 agagcctgta gcagtttaag tttataaaaa tgaaataaaa ataattaaag gagtttataa
     5581 ttgattttat ataagtatca gataatacct gagaaatatt taaatgaaat atagacttct
     5641 ttttatttga taatatttgt aattataatg cattgtatct ttatagttga gttttctgta
     5701 taacctgcta tttaaaacat actcttttat atttatacag taatgtggtt taattttata
     5761 aaatgtaatc tctactggta aaagactttg tatataaggc atttgcctga ataaaccatt
     5821 tatcttttgt atggcataga tattgctata tagaagtata tagtatgaaa ggagatattt
     5881 atatgggaga taaaatgaga gcatcagtat tgtataatgt aggtgatgtc agatatgaaa
     5941 tggtagatat accagaaata acagatacgc aagtgcttgt aaatgtaaaa tatgttggga
     6001 tatgtggttc agatttacct aggtctatgg taagtggttt gagtggaaat acaaaatatc
     6061 cattgatttt agggcatgaa ttttctggtg aagttgttaa aattggagaa aaagtaaagc
     6121 atattaatgt aggagataga gtagctgttg caccattagt accttgtgga aaatgtgatt
     6181 attgtaatga aggtaatttt ggcttgtgtg atgattacaa tataattgga acaagagtaa
     6241 atggagcgtt tgcagaatat gtaagagttc cagaagaaca tattttaaaa ctaccagata
     6301 cattggatta tgaaactgct gccggaattg aaccagcaac tatagcttat catgggattt
     6361 caaagtctaa tattagagta ggagatagtg tagttgtgtt aggatgtggt ccaataggtc
     6421 agtttgttat acaatgggca aaggtgtttg gtgcatctaa gattatagca gttgatattt
     6481 ttgatgaaaa attagaactt tctaaacttc taggagcaaa ctatatactt aactcaaaag
     6541 aagtaaatgt aatcaaagaa ataaaaaaaa taacaaatgg tggtgctgat gttgtaatag
     6601 aaactgctgg tagccgattt acacaagaac aatctctatt tgtagcaaaa aagagaggaa
     6661 atatagtgtt tgtaggtata tcacacacag aattaccact tagtgcagac gctactgagt
     6721 gtatattacg tggagaactt accttgaaag gttcttggaa ttcatataca agcccttatc
     6781 ctggaagagc atggactgca acattggatt ttatgggaaa aggtgacatt atttttaaac
     6841 ccatgatttc acataaaata ggtcttaatg aagtgggtga ctttttaagt aaaatgagca
     6901 agagagaaat taatttcaat aaaatcctag tagaaatata aaaatttaat aaaaaattct
     6961 aaaagcaatt aaattttata actatgacat taaataaaat aaaaataatt ttcttaaata
     7021 aaatatagga ggaagaaaag tgttagaaaa tttaaaagaa gaagtattaa aagcaaactt
     7081 agaattacct aagaaaaagc taattactta tacttgggga aatgtaagtg ggatagatag
     7141 agaaaaagga ttagtggtga taaaaccaag tggtgtagag tatagtaata tgactgtaga
     7201 agatatggtt gtagttgatt tagagggaaa tatagttgat ggcaaattaa gaccttcttc
     7261 agatactccg actcatctag ttttatataa agagtttgaa gaattggggg gaatagtaca
     7321 tacacattca agttgggcaa caatttgggc tcaaatagga aaatctatac catcttgtgg
     7381 tactactcat gcagattatt tttatggaag tataccatgt accagaaaaa tgactagaga
     7441 agaaattcgc aatgaatacg aaaaggaaac tggaaatgta atcattgaag aatttatggg
     7501 aagaaatcca attcattgtc caggaataat tgtaaatgac cacggacctt ttacatgggg
     7561 aaaagatgca aatgaggctg tacacaatgc agtagtttta gaagaagtcg caaagatggc
     7621 ttattataca gagttaatga gtcctgataa tattatggat aaggttctta tgaataagca
     7681 tttttcaaga aaacatggaa aaaatgcata ctatggtcaa aaatgattat ctggaggtga
     7741 attatgctag actctttaaa aggtaaagtt gtggaaatag caattaaagc agagcattat
     7801 ggtttatgta aagaaaagac tggtaatttt agtattagag ataaggatac tggatatata
     7861 cttataaccc catcaggtgt aggaagagaa gaattaaaag ttgaacatat ttgtgtattg
     7921 gatttggatg gaaatgtaat agaagttgag aaaggtatta agccaagtag tgaggtattg
     7981 atgcatattg aaatatacaa aacaagagaa aatataaatg ccatatcaca tactcattct
     8041 ttatatgcaa cagcattttc agtagcaaag aaaccaatta taccaattgt atatgaatca
     8101 gttaattatg gcggttatgt agatattgct ccatacgaaa gaccaggaac tttaaaatta
     8161 gctaagagtg tttcaaaatt actgatagat acagatgcat gcttattaga aagacatggg
     8221 ttgacaactg taggtaatga tttagacgaa gcattattaa aatcaagata tgtggaagag
     8281 gtagcagaaa tatactatag gtctttagta ttaaatcaat ttaaagaact tgaagtagta
     8341 gatgttgatg agtttaaatc ttggaaatac ccagaagaaa taaagtttta atctaaataa
     8401 ttttcattga agtggttaat taaaatatat gtattattta tatcaattac tatgttttat
     8461 tatgttaatt accgtatttt gcaaatatat ttattacatt ttttcaaaaa aatatagaat
     8521 ccgaaagcca tataaaattg acttgggatt ctatactttt tatttatgca tttttcttta
     8581 tctgattttc tggagaattt atatgttcaa aatatttttt agtttcagat actattatag
     8641 gtgataatgc aagtaaagct ataaggttag gtattgccat aagcccattt acaatatcag
     8701 caattatcca aacaacttct aactttaaga aagcacctaa taacaccata gccacaaata
     8761 cagttctata cccattcatg ccttttacac caaacagaaa ttcaaaacat ctttctccat
     8821 aataactcca tcccaaaatt gtagtaaatg caaataatgt taaactaact gtaagtaata
     8881 ttggtccaaa tgtaggaatt gctccgttaa aagctgcttg tgtcataaca gctccattta
     8941 ggtctgaaca ccacactcct gaaataacaa gagagaatcc agtaagagta cagattataa
     9001 tagtatctat aaaagtacct gtcatagata taagaccttg ttccgctggc cattcagttt
     9061 tagcagcagc agcagctatt ggagcactac ctagaccaga ctcatttgaa aatacaccac
     9121 gagcgatacc atttctaatt gctagtgaca tagttgctcc taaaaaacca ccagtagcag
     9181 cagtaggtgt aaatgcactt ttaattatta acataaaagt ttcagggatt ttattccaaa
     9241 aagatattaa aatgattcca cagattacaa catatataac tgccataaaa ggtactattt
     9301 tagtagctac attagatata cttttaagtc ctccaaatac aactaaagct acaaatactg
     9361 ctaatacaac ccctgtaatt ctagggtcta tcccgaaact attatttatt gcatctgtta
     9421 ttgaatttac ttgagtaaaa gtaccgattc ctaaaagtgc aactagtata ccacttatag
     9481 cgaaaaatat tgcaagaggt ctccattttt ctcccatacc atttactatg taatacattg
     9541 gcccaccaga cacttgacca tttttatctt tagttctgta ttttattgca agaacacctt
     9601 ctgaatactt agtagccatt ccaaagaatg ctgctatcca catccaaaaa agtgcaccag
     9661 gtccaccagc tttgatagct gtagcaacac caacaatatt accagtacca actgtagcag
     9721 ctagagcagt acataaggct gcaaaacttg aaacatcacc actacctttg ttttcagcag
     9781 agaatattaa cttaagagct cttggcagtt tagttatttg aacaactcca agcttaacag
     9841 taagtagtat accagtacca actaatagta ccagtagggg tggtccccaa ataaaagcat
     9901 caactttgtt taaaatatca attagattca taaaaacact ccctcatttt ttttgcttta
     9961 cataatcaat agaattttta atgaagtaat tgacaggaaa taagtataat ttattaataa
    10021 tttctagtgt aataaaaaaa gctaaatcta aaaaagattc agctctggat gtacactaat
    10081 tagcaaccaa aataaaagta ttagtaaata ctgtttttgg tagataatcc ctgtcctttt
    10141 gcctgagaga ttgagcataa aagccttgcc ccttcggcgc ccataatggg tctctccaga
    10201 gttccgtaaa ttttacagtc ctacttttaa aagtacctga gagtgttact ccttcggtgt
    10261 gctatatagc aactcttcta taaaatctta accggatatt gatttgtcat agcttattgt
    10321 attaataata ttaatataag tttttatgtt ttgtcaacta aaaaattaaa aaacaaagaa
    10381 aaaatataaa aatgtaagcc tatttacgaa ttataaactt agaaagatga gtaatatacg
    10441 tgaaaatgaa ttttgtaata cacatacaaa tgaagtgtaa aataagtttt aaataaaaaa
    10501 ataataaaat cttaaataat taattagata tgattaaatt atggtaatat tatagttacg
    10561 atattaagaa aagttctatt tagttagatt ttaagcgtaa tatgagatta tgtatagaga
    10621 aaggatgtat ttttagatgg gaaaaatatt agtgctagca gaaaaacctt ctgttggaag
    10681 agatatagca aaagttttag gatcaaaaaa tgaaaaaaat ggttatatag aggggccaaa
    10741 gtatgtgata acttgggcac taggtcattt ggttacactt gctgaccccg aaagttatgg
    10801 agaaagatat aaatcatgga gcttagaaga cctgccaata cttcccaagc accttaagac
    10861 tgttgtaata aaaaagagtg gaaagcagtt taatacagtt aaatcgcaaa tgaatagaaa
    10921 tgatatagat gaaatagtta tagcaacaga tgcaggtcgt gaaggagaac ttgtagcaag
    10981 gtggataata gaaaaatcac aggtaaaaaa acctataaaa cgtctttgga tttcttcaag
    11041 tacagacaaa gcaataaaag acggatttgc aaaattaaaa agtggaaaag aatatgaaaa
    11101 tttgtattac tcagcaatag cacgagcaga agctgattgg attattggaa taaatgcaac
    11161 cagagctttg acaacaaagt acaatgcaca gctttcttgt ggaagagtac aaactcctac
    11221 tcttgcaatg ttgcttaaaa gagaagaaga aataagaaat ttcaaaccta aagagtatta
    11281 tggattagaa ttaattgcta caaagggaaa ttcagatata aaatttattt ggaatgataa
    11341 aaacaataat tcttcaactt ttagtaaaga aaagatagaa tccacattaa aaaaagtaaa
    11401 aggtgtagac ataaaaatat ctgaagtaaa caagtcatat aagaaaaaat attcacctgc
    11461 actctatgat ttaacagaat tacaaagaga tgcaaataaa atgtttggat tttcagcaaa
    11521 agaaacttta tctataatgc aaaaactgta tgaacatcat aaagtactta cttatcctag
    11581 aactgattct agatatttaa catcagatat tgttggaaca ttaaaagata gaataaaagc
    11641 tgtaaatatt agtgattatt caaagatttg taatagatta ttaaaaagca atattatagc
    11701 taataaatcg tttgttgaca attcaaaggt aacagaccac catgcaataa taccaacaga
    11761 acagaaggta ttcatgagtg attttagtga taaagagcga aaaatatata atcttgtagt
    11821 aaaaagattt ttagcagtac tatctcctcc ttttgaatat gagcagacaa ctataagtgc
    11881 aactgtagaa ggagaaatat ttaaggctaa gggaaacaaa gtaataaatt taggttggaa
    11941 agaaagttat aaagatattg aagatgaaga tgagtatgaa agtataccaa atgttttaga
    12001 aaaagatgta cttaaggtca agggattaaa gataacttca ggaaaaacaa atccaccacc
    12061 atacttaaat gagggaacat tattgactgc tatggaaaag aatggtctag ggacagttgc
    12121 tacaagagca gatataatag aaaaattatt caattcattt ttaatggaaa agaaaaataa
    12181 agatatatat ataacatcaa aaggaagaca attattagat ttagttccag tagacttaaa
    12241 gacaccagaa cttacagctt catgggaaaa gaaattattg gatatttcaa agggaaaact
    12301 tagtaaaaat acattcataa atgacatgaa agattattca aaagatattg tactagaagt
    12361 aaaaaatagt gataataaat ttaaacatga taacttaact aaaaatagat gtccagaatg
    12421 tggaaaattt atgttagagg taaatggtaa aaaaggtaag atgttgattt gtgaagatag
    12481 agaatgtaat actagaaaaa ctatatcaca agtaaccaat tcaagatgtc ctaactgtca
    12541 taaaaaatta gaacttcgtg gagaaggtga ggggagaatc ttcacttgta gttgcggata
    12601 cagagaaaag ttgtcttctt tcaacaagag aaagcaagaa gaaaaacaga aggcatcaaa
    12661 aaaagatgtt aatcaatatt taaaaaatca aaataaaaat caagagacat ttaataatcc
    12721 atttgcagaa gcattagcta agctaaaaaa caaataattt taaagttatt attatacaca
    12781 attataactt taactgtata ttagttatat gtactgatat aatatagtgt atagtttagg
    12841 catgaaagta gggctgttaa atggatttaa gtggcatatt taaatactat tgtaaagagt
    12901 gtgaaaatac atggaataat tcgagtgttg aattatttga gaatatagaa acgtatagta
    12961 aagattcaca aaaaaagagg gaaaaagaat tagataaatt gctaaataca atatcagttc
    13021 atttagagag gtatccaagt gatgctgtat tgagaaaaat gtgggtaaaa aagggcgagg
    13081 ttttcttaca aaagacattg gaaaaagaaa atatttttaa gttagaaaaa atggatgtag
    13141 aggatagaaa aaaattttta gatataacaa aacagtttat tagagatgct agaaaatttg
    13201 atgatgattt acctataggt gatattatgc aagctatgag gaatgtatgg atttcaaatg
    13261 cattacaatt attatttggt aaagaagtat attattcaaa agctaacttt gcatatagta
    13321 tgttatatcc atatactgat aattatttag acaatacaaa tatagataaa aatgataaga
    13381 ttttatttaa taactggtta gaaaaaaggc tcctgggaga acacattaaa tctaaggatt
    13441 atcatgaaag taaagtatct caaatgatag attatattga aagtgtatac cctagagaaa
    13501 agtttacaga agtttatgaa tcgttattat taatatttaa aagtcaagta aatagtttaa
    13561 aacaacatgg taaggaaaat catttgtgta aagaagattt attatccatt tctattgaaa
    13621 aaggaggttc atccgtttta gtagatggat atttaataag tggattgatg acaaaggaag
    13681 aaatagagtt ttgtatagga tatggatttt tattacaaat atctgatgat ttacaagata
    13741 taaaagaaga tttaaaatac aaccataaaa ctattattac agagatgtca aaagagggta
    13801 ctttagataa agttgtaaat aaactaataa attttactat tgagttaata gatagtttta
    13861 aaattaataa taaaa
//
LOCUS       DAELNY010000099        13847 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.099, whole genome shotgun sequence.
ACCESSION   DAELNY010000099 DAELNY010000000
VERSION     DAELNY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.099"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            44..1234
                     /locus_tag="KPE55_002359"
     CDS             44..1234
                     /locus_tag="KPE55_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0493419.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    1368..1623
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1682..2683
                     /locus_tag="KPE55_002360"
     CDS             1682..2683
                     /locus_tag="KPE55_002360"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG0493420.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            2787..3176
                     /locus_tag="KPE55_002361"
     CDS             2787..3176
                     /locus_tag="KPE55_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG0493421.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            3337..4545
                     /locus_tag="KPE55_002362"
     CDS             3337..4545
                     /locus_tag="KPE55_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0493422.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(4631..5707)
                     /locus_tag="KPE55_002363"
     CDS             complement(4631..5707)
                     /locus_tag="KPE55_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0493423.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            5936..6463
                     /gene="hpt"
                     /locus_tag="KPE55_002364"
     CDS             5936..6463
                     /gene="hpt"
                     /locus_tag="KPE55_002364"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0493424.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            6552..7484
                     /locus_tag="KPE55_002365"
     CDS             6552..7484
                     /locus_tag="KPE55_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG0493425.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            7546..8772
                     /locus_tag="KPE55_002366"
     CDS             7546..8772
                     /locus_tag="KPE55_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0493426.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            8856..9077
                     /locus_tag="KPE55_002367"
     CDS             8856..9077
                     /locus_tag="KPE55_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0493427.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            9266..9436
                     /locus_tag="KPE55_002368"
     CDS             9266..9436
                     /locus_tag="KPE55_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493428.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(9529..9990)
                     /locus_tag="KPE55_002369"
     CDS             complement(9529..9990)
                     /locus_tag="KPE55_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493429.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            10173..10640
                     /locus_tag="KPE55_002370"
     CDS             10173..10640
                     /locus_tag="KPE55_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493430.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            10683..12446
                     /locus_tag="KPE55_002371"
     CDS             10683..12446
                     /locus_tag="KPE55_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG0493431.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            12454..13062
                     /locus_tag="KPE55_002372"
     CDS             12454..13062
                     /locus_tag="KPE55_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0493432.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            13134..13703
                     /locus_tag="KPE55_002373"
     CDS             13134..13703
                     /locus_tag="KPE55_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0493433.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         5452 a         1379 c         2386 g         4630 t
ORIGIN      
        1 tagacttaac aattataaaa attttattct taggagggaa aaaatgagta agtataattt
       61 tgacagagta atagatagag taggaacaga ttgtgtaaaa tgggatttta gaactaattg
      121 ttcaacaaaa gcacaaaaag atggtttgcc tttttggatt gctgatatgg attttgaatg
      181 tgcagaacca ataatagaag cattacgtaa aaaagttgaa cataaaatat ttggatatag
      241 ttcaaatgat agtgataaat actttaatgc agtttgtgat tggtataaaa ggagatttaa
      301 ctgggaaata aatagaaaag atattatttt tagtcctggt gttgtacctg ctgttgctat
      361 tttagtaaga atattaacta attctaatga aggtgtaata gtccaaaaac cagtgtatta
      421 cccatttgaa gctaagataa agagcaataa tagggatgtt gtaaacaatc ctctgatata
      481 tgagaatggt acttatagga tggattatga tgatttagaa gaaaaagcta agtgtagcaa
      541 caataaagta ctgatacttt gtagtcctca caaccctgtt ggaagagttt ggagagaaga
      601 tgaattaaaa aaggttgtag aaatatgtaa aaaatatgac ctatggataa tagctgatga
      661 gatacattct gatttaatta gaaaaggatt taaacatact cctttacagt cattatgccc
      721 agagtacaag gataaaatag ttacatgtat agctccaagt aaaacattta atctggcagg
      781 aatgcagttg tctaatataa tagtgaataa tgatgaacta aaaaagaaat atcaagaaga
      841 ggttacagct gttggagttg caacttcacc aaatcccttt gctattgtag ctacaatagc
      901 agcatataat gaaagtgaag actggcttaa tgaattaaat gattacttag ataataatat
      961 acaatttatt gatgaatact taaaagaaaa cctaccaaaa gtaaaacttg tttatccaga
     1021 aggaacatat ctagcatggt tagattttac tgcttatgga ttaaatgaag tagaacttga
     1081 agatttaatg tttaaaaaag ctaatgtatt atttgatgaa ggttatatat ttggaaaaga
     1141 aggaatagga tttgaaagaa taaatgtagc ttgtccacag tctttattaa aagaatgtat
     1201 ggacagatta aagactatat ttgaaaattt ataaagtata ggtaaaaatt tataaagtgt
     1261 ttaaaaataa aaaaataata aacttgacaa attttaacta atcacttatc atactattat
     1321 gaataaatag aaaacataag agagctaaat tttattgaat taaactctta aagactttga
     1381 aaggaagagt agttatagaa tgtaattaaa gcgagttggt gatggtgtga gaccaatatg
     1441 aagtctataa tgaagaacat cctggagttg ctaaccgaaa ttttaaaaga gtaggcttag
     1501 tcggaaccaa accgttatca tattggggtg tatcgagtaa tatctgtaca ttgtgagctg
     1561 ggtttgaatt tatttcaaac taatcagggt ggtaccgcgg aaaagcaacc tttcgtccct
     1621 tttatatagg gatgaagggt ttttttaatg gaaaaatata taaatatagg gagtgaatga
     1681 tatgagcaca ataataccaa aggattataa aagtagtttg aacgtaattg atacacaaat
     1741 agctataaaa aaattaaaag atttttttga aatgagacta agtaatgaat taaatcttat
     1801 aagagtttct tctccgttat ttgtattgcc agaaacaggt gcaaatgata atttaaatgg
     1861 agaaaaggct gtaagctttg atataccatt tatgaataaa aatgcagaaa ttgttcaatc
     1921 acttgcaaaa tggaaaagat tagcccttaa aaaatatggg tttgaagttg gtagtggttt
     1981 atatacagat atgaatgcaa taagaaaaaa tgaagaatta gacaatatac attctcttta
     2041 tgtagaccaa tgggattggg aattggttat agataaaagt agtagaaatg aaaagactct
     2101 aaaagatgtt gtaaaaagat tgtatggagt atttaaagat actgaaattt ttgtatgtag
     2161 tatgtatgaa ggtataaaag aaatattacc agaagaaata acttttataa catcacaaga
     2221 gttagaagat atgtatccag aattatcacc aaaggaaaga gaagataaaa ttgtaaaaga
     2281 gaagaaagca gtatttataa ctcaaatagg aaaaacatta atatcaggag aaaaacatga
     2341 tggaagaagc cctgattatg atgattggga attgaatggg gatatattat tttacaatcc
     2401 tgttttagat tcagcattag aattatcttc aatgggaatt agagttgatg aagaatctct
     2461 tgatagacaa ttgaaacttg ctggatgtga agagagaaaa gagtttgact atcataagat
     2521 gcttttaaat ggcgaacttc cgtatacaat tggtggagga attggtcagt caagaatatg
     2581 tatgtatttc ttacaaaaag ctcatatagg agaagtacaa gttggagtat ggccacagga
     2641 tatgattcaa aattgtagta gtgcaggaat tgaattatta taatttaaat ataaactaat
     2701 tgataaaatt tatatctaat atgttcatca aattaattat atcggttata atgtagaata
     2761 acctataatt ataagaggtg ataaatatgg agtttttaaa aataatcatt aatatagttt
     2821 tagatatttt aaaaaaaatt ttggtcagat ttaaaaatgc taaatttggt ttggtttttg
     2881 tatttgattt acttaaacta cctgatttta tgacagataa aagaatcaat atagttgata
     2941 aaataaaggt aatttctgta ttaattttta ctatatccta ctttgtatct ggggttgata
     3001 taattccaga gatgattgca ggggcatttg gttttataga tgatgctata gttttaattt
     3061 ggagtatagg tatagtgaat gaagaaatta ataagtatag agttatcgca aaaaaagata
     3121 aacattcaaa tataattgaa aatgtagaat tttctataaa agatgaagaa gaataacaat
     3181 ttatatatgt tattcttttt tttattaaca aaaaatggtt aaattgttga cggagaggta
     3241 aaactctatt atcataataa gtggatatcc aatatttaac taagtattca atgtgcaaga
     3301 atgcttaatt taaaaaagtt atggagggta aatattatga agaaaaaaat aggtttagtg
     3361 cccaaactaa tcatcggtat catagtcggt attttaatcg gaagttatgc accagaaata
     3421 atagtacaaa tattagtaac agttagtact ttattctcag cattcctaaa atttgttata
     3481 ccatttataa ttataggttt tgtaacagca ggtatagctg atttagcaac aggtgcagga
     3541 aagttattgg ggataactac gggaatagca tatggttcta ccttaatagc aggattatta
     3601 tcatttgcag tatctacatt aatcttccca aattttattg atgcaagtgt tgcaagtcaa
     3661 attggagacc cagaggcagg aatgttagct ccaatattca caattccatt agagcctatg
     3721 gtagatgtaa cagctgcaat cgtatttgca tttgtaatgg gacttggaat atcagcttta
     3781 agaaatcatg gaaaaggaga gactttattt aatttcttcc aagaatttca aggaatcgta
     3841 acgaaaacgt tgtcaacagt gattatacct ttattacctt tatacatagc aggaactttt
     3901 gcaaatataa ctatagcagg ggaagtatgg acaatattag gggtattctg gaaagtatac
     3961 ttagtagtaa tacctttaca ttttgtatat atggcatgtc aattcacagc agcaggtgta
     4021 ttttcaggaa aaaatccagt aagaatgtta aaaaatcaag tacctggata tttaacagct
     4081 ataggaactc aatcttcagc agcaactata ccagttaatg ttggttgtgc agaaaaaaat
     4141 ggagtaagta agcaaataag agaatttgtt gtacctcttt gtgcaacaat acatttatca
     4201 ggtagtatga tatcaataac aggatttaca actgcagttt taatgatgaa tggtatgcca
     4261 catggaatag gtgtattatt cccttatatt gcaatgttag gtatagcaat ggttgcagca
     4321 ccaggagcac caggtggggc tataatgagt gcacttcctt tcctaccaat ggttggaata
     4381 ccatcagatg gtgggcttgc aagtttaatg atagctttat acttaactca agacagtttt
     4441 ggtacagcat gtaatgtatc aggagataat gcaatagcag tagtagtaga taaaataaat
     4501 gatggaatga gtaaaaaagg aaataaagaa gagcaaagtg cttagttttt taataatcaa
     4561 aaatgagtta tctcaaatag agatagctca ttttttatgg tataaaaaat tataaaataa
     4621 agtgattttt ttatttaaaa attgctttaa gagatgatat taataattta ttttcattag
     4681 gttttaatat acatactcta aagaaatact catctagacc ttcaaatgaa caacaatctc
     4741 ttattattat tttttgtgga agcaattgtt ctcgaagaga tttagcagtc aattcctttg
     4801 tcttgatttt acacaatatg aaattgcctt tagtattgta tatatctaag ctttttatat
     4861 tcttaagctc tttaagcagg taatctcttt cagtgttcat taaggaaata gtatttgata
     4921 taaaatctaa atcactaaac atgatttcac ccattttaga agctattatg tttatattcc
     4981 ataaatctag atttttattt atttcatttt tgacattagt gttagatata agaccatatc
     5041 caagccttat tccaggcgtt gaaaaaaatt ttgatgtacc tcttataacg aataaattag
     5101 aataatcatc aactaattga gtacaagagt atgtatctgt atcagtaaac tctacataag
     5161 tctcatcaat cattaaaaat gagtctgtat tttttaaaat ttctctcact tctactttag
     5221 taaatgcaaa tccagttgga ttactaggat tacagattac taccaaatca taatctttag
     5281 cattgatagt ttttataaga ttttctaaat ttatatgaaa attatcttct tcttttgcaa
     5341 agtatttttc tatagaacaa ttaattttag ataactcttt ttcatactct gaataggctg
     5401 gagagagtaa aagagcctgt ttagggttaa ttgtatgtat aaatgatgaa attagttctg
     5461 tagctccact gccaagtact atattatcta tagaacattt gcagtaattg gatatagaag
     5521 tttttaagtc tatgtaatca gggtcaggat acatagatac catatctata ttgtttacaa
     5581 tgtattgttt ggctaaactt gatgtaccaa aaggatttat atttgagcta aaatccataa
     5641 attcttcctt tgaaaagcca tacttactag ataaactata taaattagct ccatggtttg
     5701 tgctcataaa agtacctcca aaaataaaat tatataatcg ataaaaataa ctgattaata
     5761 tatgtacata tagattataa tacaaaggaa tcgttattcc aaatttttaa tggaaaaagc
     5821 aaaaaagtat ggataagctg ttaatttagg tttaaaaatg atgaaaaaga ttttaattga
     5881 tattttggta tgctataata tataagttaa tagaaggaaa gcgggaggat acaaaatgta
     5941 caaagttaca ggaaaaatgt tgacagaaga acaaataaaa gaaaaagttt atgaattagg
     6001 aaaaaagata gaagaagact ttaaaggcga agatttacta gtggtaggta tacttaaagg
     6061 agctagtgta tttgtttcag atttaataag atgtatagat ttggatgtaa atatagattt
     6121 tatgagtgtt acaagttatg gaaactcaac agagtcttct ggaacagtta aaatattaaa
     6181 agatttagat gtagatattg aaggaaagaa tgtgttgatt gtagaggata taatagattc
     6241 tggtttaaca ttaagtaatc tagttgcagc tttaaaaact agaaatccaa aatctttaaa
     6301 actatgtact ctacttgaca aacctcagag aagaaaagct aatatacctg tagactatgt
     6361 tggatttgtt atagaagaca aatttattgt tggttatgga atagactatg ctgaaaagta
     6421 tagaaattta ccttatatag gtatagttga ggatgtagaa tagatttaaa tgtgaatcaa
     6481 aagcactaat ttctaaggga gttagtgctt tttagttaat tatataaaat tttagtaaag
     6541 gagacatcta tatggatatt cagttaaata tattattatt tttccaaaat ttaagaaatc
     6601 caattttgaa ctttatattt cttgtcttta caataagcac agaagcaccg ttattaatat
     6661 taattacaac aattatatac tggtgtataa ataaaaaatg tggacagaaa atactgtttg
     6721 ctataattgg aaattttgtt gtaaatttag gcatcaaaga gtttgtaaaa gctcccagac
     6781 caataggaat aaaaggtctt gaatcattga gagtttctac tgctggtggt tattcttttc
     6841 caagtgctca tactcaaact gcaacaacat tttgggttag tattatgact atatttaaaa
     6901 agagatatct tcatttaata ggtgcagtta tggttttagg agtaggattg tctaggctgt
     6961 atttagctgt tcattggcca atagatgtga tagcgggttg gatattagga atatttttta
     7021 cagttgtatt tgtaaaaata tttgactata ttgatgataa taaaaattat attttacttg
     7081 tagttttact tataccattt ataatagtgg ctatatttct aaatagccct gaatattttg
     7141 aaaaatttgg tataatagta ggatttgtat ttggatatat ggttgaggat agatttgtta
     7201 aatttaatac agataataat aacaaaaaaa taaattttag taataaaaat aaggggagta
     7261 aaaatataat tttttcaaga atatgtaggt ttttggttgg gatagtaaca ataggaatgt
     7321 tatatattgg cattaagttg tttatttcaa tgttaatagt cactttaaat ataagtgact
     7381 ctactatgag tataattttt atgaatttta ttaagaatac agtagttgtt ttttatggaa
     7441 tagcaggagc tccagcatta tttaaacttt taaaattaaa ttgattttaa ggtatagagt
     7501 caatagttta aattaaaaaa attaagggta gaaaagagtg attatttgaa gaaggtagta
     7561 ataataggtg gtggaccagc agggatgata gccgcatcaa ctgcatgtga gaaaggctat
     7621 gatgtaacat taattgagaa aaatcataaa ttaggaaaaa aacttgcaat aacaggaaaa
     7681 ggtagatgta atattactaa cgcttgtgaa atagaggaat taatagaaaa tgtacctaca
     7741 aatggaaaat ttctttatag tgctttttat acttttacaa atgatgatgt aataagtatg
     7801 tttaataatc ttggagtaaa gactaaaaca gaaagaggaa aaagggtttt tccagagagt
     7861 gataaagcat ttgatattgt aaatgctctt gaaagacaac ttaaaagtaa gaaagtaaat
     7921 atattattaa attcgaaagt agaaaaaata atatctaaaa ataataaaat tgaaaaagta
     7981 attttaaacg ataaaaaaga aattaagtgt gattcagttg tagtagcaac tggtggatta
     8041 agttatccac ttactggttc tactggtgat ggatataaat ttgcaatttc acaggggcac
     8101 acaattatag atacaaaacc atctttaatt ggtatagaag tacaagaaag ttttacaaaa
     8161 gatttagaaa aattatcatt gagaaatgtt gaaataaggg tgtttaatag caaacaaaag
     8221 aaagtttata gtgattttgg agaacttgaa tttactaaat ttggtttaga tggacctata
     8281 atcaagtctg caagttgtag aatgaaagat acaaggaaag aaaattacac tatattatta
     8341 gatttaaaac ctgcattaga tgaggaaaaa ttggataaaa gagttcaaaa agattttcag
     8401 aagtatacaa ataaaaaatt tgaaaaagca ttagatgatt tacttccaaa aaaactaata
     8461 cctataataa taaacttaag tgaaattaat ccaaatacgg ttgttcacca aatttctaga
     8521 gagcagagaa aaaacttagt ccatttatta aaaaatttaa agtttacagt aaagagatac
     8581 agacctattg aagaagctat aataacttct ggtggtgtaa aagtaaacga aataaattca
     8641 agtacaatgg aatcaaaatt agtagaaggt ttattttttg ctggagaagt aatagatata
     8701 gatgcatata caggtggatt caatttgcaa atagcttttt ctactggata tttggcaggt
     8761 tttaattgct aaaaattacc tgaattaaaa atgtttaaaa tcacataata ttatcagcaa
     8821 cacaaaataa attaaacaga aggaaggtaa tcaacatggc aagtaacaat aacaacaaca
     8881 gaacagtagt accaggagca aaagaggctt taaaccaaat gaaattagaa atagctaatg
     8941 aacttggtat gagtaattat caacaagttg ataaaggaaa ccttactgca agagaaaatg
     9001 gttatgtagg tggatacatg actaaaaaat tagttgaaat ggctgaacaa caaatggctg
     9061 gaaaaagcaa cagatagtta taaataatta tttataacta ttggctatta gtttagctag
     9121 agctatctca aatatagaaa gtaatttcta aattgagata gctttttttt attaaattat
     9181 taaatagata tatttttatg taataaattt tatgtatttg gataaaataa tacaaatgaa
     9241 tatgttacat aaggagtgat gtaaaatgag tagtgtaaat tttgaggatt taaaaaacaa
     9301 atttattaat agtgacttag atgaaaaaat aagaatatat acaacaacag aaggtttatc
     9361 agtagaacaa tttaaagagc tactaaaata ttatccaata caacatttat ctaaactaga
     9421 aaaagcactt ggatagcagt atttaatcca aaattaaaaa ggcaatttat ttaatattaa
     9481 aaatattttt tattaaataa attgcctttt tataataggt ttaaattatt atgcttttga
     9541 tatataatta tagattctct catttccaag ttcaatagac ccagtattaa taagagtaac
     9601 tattccttct atatctctag cagttaagta tttatatgga attaataaat tataaccagt
     9661 attacttagt aagtttttta attgggttat ttgagagata ttgaaacatt ttgcagtttt
     9721 agtagatttt tttcttaagt tagataaatt ttcattttga atatttatta aagtttctat
     9781 atagtttcta taagatatct taaagtttgt ctcattgact attttttcat taagtatact
     9841 actgataaaa tcttgagaca atatattatc tgcaaattta tctgctgtta attttccaga
     9901 tgttatgcct ttttgatatt ttctaagaat ttttttagta tagcagtata ttttatttct
     9961 cgtttctaaa tcgagttgtt ctatttgcac aaaaccactt ccttctaatt ataaaaatat
    10021 ttattaaaaa tctatactta actaataaag cttcaataac ttatttacaa taaatactaa
    10081 atcataaaat atttgttatt ttttattatt accaagtata aaaaatatta taattgtaaa
    10141 acttacaaat atatgtaaaa tatttaggag tgatgaaaat gttaaatggg ataataaagc
    10201 ttttttattc tgaaattgta aaaacaaaga ttaataactt agattcaagt ggatattctt
    10261 taagagatga atgtaaagaa atcagaaata taactctaaa gatagatagt gtatttttta
    10321 agcttttaaa gttagctttt ggttcaatat tgcttatggt agcttttaat ttaaacatgg
    10381 ttttaggtat aggagtgttt ttagttggtg tagtttatat actatataaa agaaatatgg
    10441 aagaacaagt aaaagaacat ataaataatg tgaaaaataa tatcgacatc tctaaaataa
    10501 cttttatgga tgaaaaaggg agaacaggtc taaatgcatt aattacatta cttcttatag
    10561 gattattgac aggatttaac tggatattag ttgctagttt tgtcgttgtt tttgttttta
    10621 ctataaagaa tgtatgttaa aatactaata aatagtttaa tatatagggg gagaaattga
    10681 gtatgactaa tggcagttat aaaatgaaga atttagtgga aatatccgct atggtgactg
    10741 aatctacaga tttttttaat ataaaagatg acataataga aaaaatgtta gaagttgttc
    10801 accctacaaa ggcatgtgtg aatctatttt ataaaaatga ttctaaatat gcctacttag
    10861 tatgttcgca aaccttagaa tatgtacctc aaatttttcc aataaatagt cttaaaggtg
    10921 caaaaattga ttttgacaca tatccagagt atatacatga ggctgtaaat gaaaaaaaga
    10981 taatatatgt ggaaaatgta tttgaagatt ctagagcaga aggtgaacga gatttagcaa
    11041 aagctgaagg ttatattggg agaatagttt ttccttttat aataaataac gtagttgttg
    11101 gatttatgac ttgtttttta aaagaggatg attatataac agaacaagat atagatttta
    11161 tatcatctgt tgcatcactc ttaagtttat caatagaaat aactaataaa aataataatg
    11221 tacaaatttt aattgataaa ctaagaggct caataagtgc cataaatgaa gctactaaaa
    11281 aactatacct taataagagt attgatggct ttttggaaaa tttaagtaaa caagcgtgta
    11341 atattactaa tagtaaagaa gctttaataa ttataaatag tcatcataat aaggatgcta
    11401 tagttagttg ttataatgtt gagcaaaaaa agaaaacaaa tttatatcct atgattgata
    11461 tgtttttaaa gaaagagtca ttgggggggt atctaaataa tttaaaacca tgctcaaaaa
    11521 agggaataaa tttggaaagt tatatatatt acaaacttca ggataaaaat gatactatag
    11581 gatgtatagt ttgtgcaaat agcaaaaact atactagtga tgatttaaat attcttagta
    11641 tattgtccaa acaagtttct gttggtatgc aattatatga acataatgaa aaggaagtaa
    11701 aacacaaagt tcttgaaaac gagttgaaca tattaaataa acaacaacag cttattatgg
    11761 ataaaggtaa aatggattgt aatgatgaga aagaacttta tttttaccat aaaccagcaa
    11821 gggtagtagg tggagatttt tattatgcta caaaagtgga tgatgaacat atagtttata
    11881 ttgttgctga tgtaatggga catggaatgg tatcaaatta tgttgttgca atgataaaag
    11941 gtgcttttaa agtattatgt aatcaataca atacaccaag agaaataatg acaaatttaa
    12001 ataggatgtt atatgatgaa tttgacaaga tgggagtatt tacaacttgt ttgataggta
    12061 ttatagatac taaagaaaat atgattactg tgtctaatgc aggtcactat agtcctgtag
    12121 ttgtaaagaa agatggcaca atagaaaata atttaaattg taaaaagggc atacctattg
    12181 gaataatgga agatgctact tatgaaaata atactctttc aatggaagat tatgcaatgg
    12241 tatgtatgta cactgatgga attttagaga taaaaaattc atcaaaagaa gagtatggta
    12301 taaaccgatt agaaaacttt ctaaaagaaa attttagatt aaatcaacag ttaatagtag
    12361 aaaatctaaa attggattta aagaacttct catcaaaaga taattatgat gatgatattt
    12421 taatagttat gttaaaggat aggtagattg attatggatt caaacgataa aaaaggactt
    12481 aaaagtccag agattataat aataaatgga agtccaagaa ttaataaaaa ttgcagtttt
    12541 atagcaaatg aagtaaaagc tatgctagaa gagtgtggat taagttgtaa aatatttaat
    12601 atctacaata tggatattga atattgcaca gcatgtggat tttgtgaaaa aacagggtat
    12661 tgcaagttta atgatgatat gaagccatta tataaaatgt ttgatgattc aaaaggaact
    12721 atagtaataa gtcctgtaca ttttgattca atatcagcta aattaaagac tgttgttgat
    12781 agaactcaag ctatatatgc aagtaaatat gtattaaata aaccttcaat tgatagaagt
    12841 aagaaacgga taggaatgta tattgcagtg ggagggagta aatcatatga ttcacagttt
    12901 aaaggtggac agattgttat ggattttttc tttaagagta taaatactaa acttttacac
    12961 aatatatatt taaacaatac agatgatgta tcttatatag ataatcaaga atttaaagat
    13021 ataatatata aaaatttaaa gatatatatt gattcaatat aatttgattt tgtaggtaat
    13081 attatgtata attgtattgc aataatttaa ataattaaaa ggagggaatt tcaatgaaat
    13141 taccaatagc attatctaat aaacatgttc atttgagtca agctgatata gacgtattat
    13201 ttggagcagg tcatgagtta actaaattta aaccattatc tcaaccaggg caatatgcct
    13261 gtgaagaaaa agtggattta gtaggaccaa aaggaacttt aaaaggagtt agagtactag
    13321 gaccagctag agctcatact caagttgaaa tatctttagc agatggtttc ccattaggag
    13381 taaaagcacc aataaaagaa tctggtgata tagctggaac tccaggtgtt aaattagtag
    13441 gacctgctgg agaagttgaa atgaaagaag gagttatagt tgcttctaga catatacata
    13501 tgagcaatga agatgctgct aaatttggtg taactgataa gcaaatagta aaagttaaaa
    13561 catctggacc aagagcatta gtatttgaaa atgtattagt aagagctagt gacagcttta
    13621 atctagaaat gcatgttgat atggaagagg gaaatgcagc aggcgttaaa aatggagatt
    13681 tagtagaatt aatagttgat taatattata aatagacttt atgctagtag atattttatt
    13741 tgctagcata tttttaaatg taataaattt ctataggata aatttaatcg attagattaa
    13801 tttataaaat aaaggggtat tataaaaaat gtaataaaaa cttacaa
//
LOCUS       DAELNY010000100        13613 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.100, whole genome shotgun sequence.
ACCESSION   DAELNY010000100 DAELNY010000000
VERSION     DAELNY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.100"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            256..3354
                     /locus_tag="KPE55_002374"
     CDS             256..3354
                     /locus_tag="KPE55_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0493434.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLASLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3477..4763
                     /locus_tag="KPE55_002375"
     CDS             3477..4763
                     /locus_tag="KPE55_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0493435.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5142..6248)
                     /locus_tag="KPE55_002376"
     CDS             complement(5142..6248)
                     /locus_tag="KPE55_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0493436.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6283..7824)
                     /locus_tag="KPE55_002377"
     CDS             complement(6283..7824)
                     /locus_tag="KPE55_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0493437.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8186..8932)
                     /locus_tag="KPE55_002378"
     CDS             complement(8186..8932)
                     /locus_tag="KPE55_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493438.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9144..10454)
                     /locus_tag="KPE55_002379"
     CDS             complement(9144..10454)
                     /locus_tag="KPE55_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493439.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKKEYLTFLEKHDKTL"
     gene            complement(10467..10937)
                     /locus_tag="KPE55_002380"
     CDS             complement(10467..10937)
                     /locus_tag="KPE55_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493440.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10941..11792)
                     /locus_tag="KPE55_002381"
     CDS             complement(10941..11792)
                     /locus_tag="KPE55_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0493441.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            12716..>13613
                     /locus_tag="KPE55_002382"
     CDS             12716..>13613
                     /locus_tag="KPE55_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0493442.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSK"
BASE COUNT         5151 a         1767 c         2084 g         4611 t
ORIGIN      
        1 tggagatttg tcatttatat gagaattaat tataaacaca taatgagata attaattata
       61 aatatattaa attaggtatt acaaaactct attgtacgat tgacacaata ataattctat
      121 ttactaaaaa atagaacctc attaaggtac tattttaaaa cataaagaag tataatatca
      181 ttgccaaata tatagcaaaa ttttaaaagc tttaaaaatt caatctggaa aatgaataaa
      241 aggagatata tataaatgga tgtaataaat ttaatagatg agttggatga aattgtatat
      301 gtttcttctt tagatacata tgaactttta tatattaatg aaattggtaa aaaggcaatg
      361 ggacttaatg atatatccca tttaaaatgt tataaagcat tacaggggaa agattctccc
      421 tgcgaatttt gtacaaactc tattttaaaa gaagatgaat attacatatg ggaaaatacg
      481 aatactcgaa caaatagaca ttttatatta aaggacaagt taattaaatg ggagggcaaa
      541 catgcacgat tagaaatagc tcttgatatt acagaaaaag aagatataag taagtcaata
      601 gcagaaaaat tagagataaa ggagttatta gttggatgta ttaaacatct tataacagaa
      661 tctaattttt ctgatgctgt aaatcttgta ttaaccaata ttggtaaatt tcataaagct
      721 gatagagcat atgtttttga agtatcagaa gatagaaaaa aagtaactaa tacatatgaa
      781 tggtgcaatg aaggtgttga tgaacaaaaa caggcactaa caaatcttga tattaatgaa
      841 ttaaagaatt ggtttttatt attcgaacaa aaaggttttg taataattga tgatatagaa
      901 aatataaaag aagaacagcc aatagaatat gaaattttaa aacctcaaaa aattgaaagt
      961 ttaatgacat cttcactaaa acaagatgat aaagtagtag ggtttattgg agttgataat
     1021 ccacataaag ctgttggaga tacctctctt ttgacatccc tatcttattt tttattaact
     1081 gagatgaaaa aaagaaaagt agaaaataaa ttaaattata tgagctacta tgacttcttg
     1141 actggtgcat ataatagaaa taaatatatt aagtatatag ataattttca aaaaggaaaa
     1201 gttaaatcca taggagttgt atttgtagat ataaatgggt taaaagatat taatgataac
     1261 tttggacatg ctaatggcga ctctgctatt gtgtcagcat gtgtaataat aaaaaaatac
     1321 tttcattctt ataatgttta ccgtattgga ggagatgagt ttgttgtatt atgtgaagat
     1381 atatcaaaac aactatttga agaattagtg agttggttaa gtgaagaatt taaggatgag
     1441 atgatatata gtgtagcagt gggcagtatt tggagtgacg ataatattga tatacataag
     1501 ctaattaaag ttgctgatga gagaatgtac caagataaga agaggtatta taataagaac
     1561 aaacttagaa ataatgaaat gaaagtaatt agtgacttag ctgattcgac gaataatatt
     1621 ttatttaaac ataaagagtt agttgataag gtttattttg atgatttaat gataaaaggg
     1681 tattctaagg cttatgaaaa attagcttat aatacatatg atataatttt atctatttgc
     1741 attgatacga atttaattac tccaatttat caatcagaga gcaatatttt tagttttcct
     1801 ataaaggaaa atcttgaaaa aactttttca tacataaaaa aacatttaat tcatcctgat
     1861 gatataatat tatttaatga taaccacctt ctaaaatctc ttgctagtct taataatagc
     1921 aagcaagacg atttagaact aaattttgag tatagacgtt taggaataga taaaaagtac
     1981 tattgggcat taatttatat gttaaagatt agtagtaaaa ataagttagg tgagaaacaa
     2041 acacatattt tagttgctat aaaaaatata gattatctta tgcaactaca taaaaaccaa
     2101 aaagatacat taacaggact atacaattat gaaagttttt gtgaacaaat aagtttaatg
     2161 cttaaaagat atcctgacaa gcgttatgca attataatga tggatataga taagttcaag
     2221 gtaattaatg acatttatgg agttagagga ggagataaag ctttaatata tgttgcagag
     2281 ttgattcaaa aaaacattgg ttctaatggt atatgttgtc gtatgtatgc agatgtattt
     2341 tgcatttttt atgagagtta ctctgataga gatattcata aggtaattta taagattaaa
     2401 aatagcttaa atagaaaaaa atttgaatat atgttagtac catgttttgg agtttataga
     2461 attttggata atcatattcc aattacaaat gcgtgtgaaa tggcaggata tgctcacaaa
     2521 aaagtaaaga aacaaagtat caacaactat atattttatg atgatactat aagaaatgat
     2581 gcacttgaag aaaaacagat ggaaagggaa atggaatacg cacttgaaaa tgggcaattc
     2641 catgtatatt tacaacctaa gtataatctt aatgcaaata aaattgttgg tgcagaagca
     2701 ttggttagat gggttcatcc agaaaaaggt ataatacctc caattaaatt tatttcacta
     2761 tttgagaaga atgggtttat tatcagatta gatatgtatg tatgggaaca agtatgttct
     2821 ttgattagaa aatggataga tttaggtgaa gagccagttc ctatctctgt aaatgtatca
     2881 agattacatt tgcataatcc acattttaaa gaaattgttt taagtttaat aaataaatat
     2941 agtataccaa aatctttttt agaattagag cttacagaaa gtttatttat taaaaatata
     3001 aaattattta ctaagataat agccgacctt agaatggatg gtattgtttt aaatatggat
     3061 gactttggtt cagcatattc ttcattaaat atgttaaaaa atattaatat tgatacatta
     3121 aagatagatt gtggtttctt taatgaggaa ggaatcacag acagagaaaa aatagtttta
     3181 aagcacacta tagccatggc aaaagattta gatatggatg tagtggcaga aggtgtagag
     3241 acaaaagaac aagctgaatt tttagtgagt ttagattgtg tggtaattca aggttattat
     3301 tattgtaagc ctattccaag tgctgatttt gagaagttac tttatcaaaa ataagctaat
     3361 tattccattc taaatgtagg taggatggat tagttcaaaa taattaaata gaagtagaaa
     3421 atagtgagga tataattttt aaaaagtata atatttatgg attaggagcg aagtttatga
     3481 tagtgggtat tataggtcct tcagactctg ggtttaagat aaaggatgat ttaaaacaga
     3541 tagacagtga tttaaagact aaaatatatg taagagaaaa agtagtagat acaatagaag
     3601 ttatttctaa atgtgaagac gaatgtgatg ctattatatt tacaggatgt gcagtttatg
     3661 aatttataaa atctaagtat gagataagta agccacattc atttgtaccg agaagtggga
     3721 cgagtataat gaaggcattt tgggcaataa aaagtgcaaa tataaaatta gataaattca
     3781 gtatagatgt agttgataga tatgttgtgg aagatgcact taaagaattt gatattaaag
     3841 caacgagtgt attttgtaat ccattttctt tggaagtagg agagtctgaa cttgtagaat
     3901 ggcatataaa attatttgaa gaaaataaga cggatataat gttaactggt tttggtgcag
     3961 tatataatga attaaaagaa agaggctatc ctgtatttcg tttacaagct actatacaat
     4021 taatcaaaga aagttatgac aaggtaaaga gtgaatatgc tctaagtaag gcaagatttt
     4081 ctcaaattgc tgttgaaata ttgagtttaa tagattataa agagaagata gataactatt
     4141 attctgacat gataaaaaaa tcagatatgg ataagcttgt agtaaactat gtaagaagca
     4201 ttcaaggttc cttattttta cttggaagaa atgattatgt tgtatttgtt cataaagggg
     4261 tagctgataa tgagtataat tatgataagc tattcaatct aaagagagag ataaaagaca
     4321 tgggcttttc tcttagtata ggaattggta ctggagtaac tgcgtatcag gctgaaaata
     4381 atgcacataa agcattaaga cactccatag attcaaaaga aattggtata tatttggtag
     4441 atgaagatga aaacataaga ggacctttag cttccgaaaa tgaattaaat tattctttga
     4501 tgctatctga tgaaaaggta atcgaaatat ctaaaaagac tggaatgagt tgtgagtcag
     4561 ttgcaaaaat tattgctata aatgagaata gaaaaagtaa gatttatgat tcaaaagagt
     4621 tagcagagta tctaaatgta agtgaaagaa gtgcaagacg tatattaaat aaaatagtaa
     4681 atgcaggttt aggtcgaata tgtgcaaaag agactagtat tggtggagga agaccaaaaa
     4741 atattactga aatactgttt taagatattt tataaaactg tataaatcat tatattgtat
     4801 aaaattctac aacaaattac tatataaagt attaaagctt attatgaaaa aaattagaac
     4861 aaataattat gtaaaatact agaataaatt attaatataa aatactagag cctattatta
     4921 aaaatgctag aataaattat tatgcaaaat attgaagctt attatgaaaa atgttagaac
     4981 aaattactat ataaaatatc aaagcttatt atgaaaaatg ttaaaacaaa ttattatata
     5041 aaaatactaa aacctatgta aaacaactct aatatataac ttataaagtt gaggatacat
     5101 aggtttttat ttttataatt taaagagttc tattatgata tttataattt aatcatatga
     5161 taaactattt tggcagtatc atataattcg tctatattta catattcatt aactgtgtgt
     5221 acatcataca tacctagact taatatagca caattatatc caagattagc taatatattt
     5281 gcgtcacttc caccaccaat taccattgga tttggaacaa caccaacttg tcttattgct
     5341 tcttcactta acttgaaaac atgactatct ctactaagtt caaaactagg gtaagacata
     5401 ttatgttcaa aagtataagt agtattaaat ttagatgcag cgtccttaca acatttttcc
     5461 atatgattaa gctcatactc taaagtttca ggtatatgag acctaatttc agcagtcaat
     5521 gtaaccttat cagttactat gtttgtagct ccaccacctt ctatttcacc tatatttgaa
     5581 gtagtcaatg aatctattct gcctatgtgc atgttgctta ttgcatgaga ggctactaat
     5641 atagcattta accctttttc aggttctata ccagcatgtg ctttctttcc ttgaaaagag
     5701 atttttatag attgttgagc aggagctttg tatgctatcg aaccaatagc tccaccagaa
     5761 tctaaaatca ccatatcttt acaaggcaag ttgtcaggat taaagttttt gacaccatgc
     5821 ataccagctt cctcacagat tgtaaaacaa agataaatat ctctatgtgg tattttttct
     5881 gtaataacat gttctaaagc ttcaagtata gcagctattc cagctttatc atctgcacca
     5941 agtgtagtag ttccatcact tctaactaca ttaccatcta gtattggttt tacacctaaa
     6001 caaggttgaa cttggtccat gtgtgcacat aaacaaagtg gtctacttcc ttcttcacct
     6061 tttatatagg caatgacatt acctgtatta ccaccatatt tttcacctgc atcatctata
     6121 atggcatcta tatttctttc ttttaagtag tttaccaacc acttagacat ttctaattct
     6181 tgattagatg ggctatcaat tctaaccata tccataaaat tatttataag tctattttta
     6241 tctaacataa attatttctc cttttagaaa gatttaaata aattacatgc tatatgaagg
     6301 aagtattcca ggtcctagtg gaatattaaa taccataaac aatattagta gtatagtcca
     6361 cactattaag aatgatatag agtatggtat catattagca attacagtac ccataccagc
     6421 atctttatcg tattttcttg tgaatgctag tataataggg aagtacggaa ataatgggct
     6481 aagtggattt gttatacaat cacctatacg ataagccatc tgagtaagtg ctggattgta
     6541 tccaaccatc ataaacattg gtacaaatat aggtgcaaga atagcccact ttgcagatgc
     6601 actacctata aataaattta taaaacagga taatataata aatcctatca acaataaagg
     6661 tccatttatt cctgctgatt ttaaaaattc tccgcccttt acagctagta tcagacctaa
     6721 attactattt gtgaataatt gcaaaaattg tgaagcagca aatgctagta ttatatatcc
     6781 acccatatca ctcattgcac tagccattat agagactaca tccttatcct tctttatttt
     6841 acctatagtt tttccgtata caacaccagg tatgaaaaat gctagtgcta ttataggtac
     6901 catacctttc ataagaggag cattgtttga tagtaaatct cctgtttctg ggtcagctaa
     6961 gaatggtttt ttaccgatta cactaagagc tactataata gccacatata ttaatatact
     7021 tataccagct tttttaagcc ctttcttttc tacatcacta agacttccat caacatctag
     7081 ttctgcattt ccttcatatt taccaaatct aggagctaca attttttctg ttacccatgt
     7141 tgccaaaagt attagaacaa aagttgatgc tatcataaaa aataagttca ttgttgcatt
     7201 ggcttgaaat ccagaatcaa tcatttgagc tgctggaact gtaaaacttg cagcaagtat
     7261 atcattgaca cttatcataa tgtttgcggc aaatcctaga cagactgctg catatccaga
     7321 aaataaacct ataagtggat gtctaccaac accaagatat acaagtgcag ctaatggtgg
     7381 aaataatatt gtacctgcat ctgaagctat attggcaagc atacctatag tcattacaac
     7441 aagagttact cttgtttcag gaatcttgct tactgatgct ttgattgttg ctgtcataag
     7501 tcctgttttg tcacatacac cagcacctat catagttact agaactagac ccaaaggagc
     7561 aaacccttgg aaattgctga ctatgtttcc taaaagaatt tgaagctgtt ctacagtcaa
     7621 taaatttaca gcggttatag tttctccagt accaggatga actactgata tccccataga
     7681 accaactaga ctagatagta taagtaaaag tacacataac cctaaaaata ttgtaactgg
     7741 gtctggtagt ttattaccaa cagtttctat tctgtttaat gtcctgtcaa aaaagttttt
     7801 cttcttaact acgttatcat tcataatatc ctccctaatg tttaaaaata taattaaaaa
     7861 ataagtattg tggttaatct tacaatttaa attctgcttg tcgatttaag acgtgtccac
     7921 aatatgtcct aaaaaaagta taaaataaac tttttcctgt gtcaatgcag ttttctgaat
     7981 tttctggttg agtggtgtta gttaaatatg ctttaagtag attaatgacg aattatatct
     8041 aatgtattta aataacatat gcgctttaaa attaatttta aaacaaaatt ttatgaaaag
     8101 ctagtggaag attaagttat taaaataagt agcgtagtaa agtgttagat tttttaatta
     8161 atcaaagaaa tttactttgt attttctaca aaaatctgtg aatatctttt gaattatgta
     8221 gaggatataa atttatttta tttacataga ttagattttg ttctaattta ctgtattcaa
     8281 atggttttcc atgtccagga taaatcattt taggttttgt attgataatt gttttccaag
     8341 attgaataaa ttctagtttg ctttctgcaa atatggtaat tttgtgagta cttggaaatc
     8401 cattcattgc agaatctcca caaaataaaa caccatcatc aattagtagt gaaattgaat
     8461 catcagtatg tcctggtgta tctataattt ttccatttag ttctttttca atttctttac
     8521 gattcttgtc agacacaaga atacaccttt tttcaaattc ttgtttaagt ggagaaaaac
     8581 tatgctcacc ttttccaaat agtttcataa tttggcaaaa gagaaaggca gtacggctgg
     8641 tacaaccacc aataaaagag ttttgacctt tatatagaat ttctaaagct ttatcatgca
     8701 taacaatctt tgtattagga gattcgtcta gaatctgatt tagaaaacca acatggtcat
     8761 catgagcatg agttagaaat atgtatcgaa tatctttgat ttttatgttg aatttagaaa
     8821 ttcgtttttt gaaatgttta aaaccatttt catatccagt atcaatcaag acaaagccat
     8881 cttgaatagg gtacaaccat gtatttataa ttcgatttcc tgcattaaac attttttcac
     8941 caccttgaat tagaatagaa atttatatta attagaaaga atatagcata gttctactga
     9001 tatttcaacg tagctctaaa agttttaagg aggataatat attttagttt aatctaaaaa
     9061 ctaaaagaaa agaagtatac tgttacaaaa acaaagagtg gtataacttt tacagtagat
     9121 accaccctat tataaaaata gacttataaa gttttatcgt gtttttctaa gaatgttaga
     9181 tattcttttt tagtcataac tggagtaaag gtatctaata tatctttagc atccttagca
     9241 ttatcaaaaa gaaggtctac aactgtaagt gccattgctt tagcaggaat tatgtaagct
     9301 aaatcttcat caactatttc aaaatctctt gtatgaagag ctccttttac accaccaatc
     9361 atagggtgaa gagtaggcat tatatgagac acatctccaa aatcaaaaga gcctgtaaaa
     9421 tctccaccat caataacttt tccttttaga cctaaatctt caagattgtt tttaaataaa
     9481 ttatcaagac ttttgtatcg taatattgga agatatccag gaatttcagt tatctcaaca
     9541 tcagctccaa ctgccattcc accagccatc aaagccttat ttattctttc attagcatca
     9601 atcataccat ttatagttct agctctaaca tatgattcca tatgaacatc agcaggaaca
     9661 acatttacta tatctccacc ttttgtgatg atagggtgga atctaactct ttcagactct
     9721 ttaaaagttt ctctaagtgc attcacgttg tttatagcaa gcattgctgc atttaaagca
     9781 tttattccat catgtggagc agaaccagca tgagattctt tacctataaa tttaaccttt
     9841 tttccaacaa atccattact ctcaggacca actagagcct tatcttctcc taaatcagaa
     9901 gcatgaaaca tcatagatat gtctatatca tcaaaataac ctctttttat taattcttgt
     9961 tttccaccaa aataagttat ttttccttct ttttttaatt gctctctgta ttccaactca
    10021 ataaactctt cagcaggagt agccataaaa gatactttac catctaaatg ttctaaaacg
    10081 ccacttttta ctaaaccaac agcagcacct aacattccag ctatttgaat attatgtcca
    10141 caagtatgag aagcacctat ttcattagca tctttatgtt ctttacatga tataccatcc
    10201 aattctccaa gtatagatat atgaggacct ttcttgctag aatttgcatc tgctttgcat
    10261 cctgtaactg ctatattttt ttctacagat aaatttaatt cttgctcaaa aaagtttgaa
    10321 acagcttctg tagttttaaa ttctttatat ccatattctg gatttttgta tattccttga
    10381 cctattttta atattttttc tctattttca tctatagttt ttattactag agattttaat
    10441 tgttctttat tcataataaa ttcctcctat attttacctt gaatacttaa aactatatga
    10501 gctatactcg cacatgcaaa gaatatagtt gtaaatacaa taagtgaaat tataactatt
    10561 ttccatgaca tttcttttaa agcttctatt ttgtttccaa cagaaattcc tgcaaaagct
    10621 agtagtggag tagttatagc cataaagtta atattattag tgttagttaa gaatacttca
    10681 gatgttggac tatatggcat actaagtaaa aggccgatta tagttgcaaa tccaaaagct
    10741 ggaaagattg atttaggaaa taaatctttt ataatcatag ctgccatagc agctaatatt
    10801 agcatcacca taccaggaag agctgtaact atagatgtac caacgctaat tctttgtcct
    10861 ataagaatta taactccaat aacagacata accaataatt gatatgaact tttctttaaa
    10921 tttaaattag caaacattta ttagcactct ccttctgtag atttaatttc tttagttttt
    10981 ttatttctcg aaaattttgg ttctaaaatc ttatacataa aattagtaaa tggtaaggat
    11041 acaaaaacta agaatataat tcctgttata ctagacatca tattgcttgt tgcagcatag
    11101 gcaagtattg tatctgccat ttcaggtggt accatggtag ataatgaacc agcagctgct
    11161 gtcatcatac ttccactacc aacaccacag gccattccca aagcatatgg gtgaagacca
    11221 ctataaatag atattgaacc tagtacacta aagtatatag taccaagaac tgttcccatt
    11281 aaatatgtac caagtacacc actaccttct ggtgagttta tcccatattt ttctgatata
    11341 actcctaaag aaggttcacg acttatactt gcagtagctc caacagcttc tcttttcatt
    11401 ccaaacatta atgcaagagg taatgcaacg attggtgcaa gtatatgacc aaattcttga
    11461 gcgataaacg ctggtccagc ttgaattatt ttgtcaatgt ttggtccaac tgtagtacca
    11521 tattttatac caagcattac tagtgcaatt ccaacaactt caccagcaat atttatttct
    11581 ttttctccaa ccactttttt aagtgcttta atttttttac ctaataaatc tggtgttatt
    11641 ataagtccaa ttataactgc ataaagcata gggaacagta ctatagaggc ttttccaata
    11701 gctatagttc tttgccctat aaattcacat attactacca agattaagca agttaaaaat
    11761 acttgaataa aatgtttttt attaatttcc atgatttcct ccccaaaaga ataaatattt
    11821 tacttatctg aaatgagaaa atacgttttc ttaagtataa tacgttttcc taaataaaat
    11881 gaaaaaacta tttcaaataa gtttcagaaa tgtcaataac atgtccttaa ttaaggataa
    11941 aacaaaaatt taacattgtc aacgaaaaat atctaaaata ttacatattt tgaatatatt
    12001 gtaatatagc tttattatga aaaaattgtt taaatattgt aggaataata gctaaaaagc
    12061 tttaaaatta gtaatataaa attgaaaaat aataatatag agttgagaaa tagtaaattg
    12121 aaattttaaa ataaatgagt ggaagtagct tagtaaagta tggaaatgaa tattacaata
    12181 gaaaataaat aatatgtaaa gactttaaat taattttaag tattaagtac aaatactaat
    12241 tttgatatag aaattgaata aagtaaatga taataagcaa atgaattaag atgataataa
    12301 gtgaattgat atagaaataa gtaaattaag taagacgata acaagtgaga taataataaa
    12361 tgaattgaca tagcaataag taaattaagt aagacgataa caaatgagat gataataagt
    12421 aaattgatat agaaataagt aaattaagta agatgataac aagtgaggta ataataagtg
    12481 aattgatata gaaataagta aattaagtaa gacgataaca aatgagatga taataagtaa
    12541 attgatatag taataaataa attaagatga taatatccaa agtgagaaag ataatagaaa
    12601 gtaaataaaa tcatgtaaaa ataatttaga taagattcta agattttatt tatttaaaaa
    12661 ttagtatgat taatttagat taaataaatt taataatttt agtaggtggt aatgaatgaa
    12721 aataaataga ttaacagaaa taatagtaat tctattaaat aaaaaattag taacagctaa
    12781 agagttggca gatagatttg aagtttctac aagaaccata tatagagata tagaaactct
    12841 gtctatgtcg ggagtaccag tatatatgac gaaaggcaaa ggaggaggga tatctttaat
    12901 agaagaatat tctatagaca aggctatatt gtcaaaaaaa gataaagaga gtttaatagt
    12961 tgcactgaaa acattacagg cgactaagta tccagaaata aattcagtag taaataaaat
    13021 tggctcaata tttggagagc agaatttttc aaactggatt gaaatagact ttactgaatg
    13081 gggaagtaat tttaatgaag atgataaatt tacaaaaata aaagaagcta ttttaaggcg
    13141 taatactata aattttaatt atgtaaatag tttgtcaagt cagacaaaca gaactgtcga
    13201 accactgaag cttatgtata agagtaagac ttggtacttg tatggatttt gtaaactaaa
    13261 agatgatttt aggatattta gaattagtag gataaggaat ttatctataa aggatgaagt
    13321 attttcaaga aagataatag aagaagtatg tttgaatgat tctaaagtaa ttaaggaaaa
    13381 tactataacg ttgaaattga ggtttaaaga aaagatgctt tttagagtat ttgatgattt
    13441 taataaggat ttaataacta aaaatgaaga tgatacatat gatgtaatta cagaatttcc
    13501 tattggtgaa tggatttatg gatatatttt atcatttggt gacaatgttg aagtacttga
    13561 acctaaagat gttagggata atgtaataac tagactaagg gagttgtcta aaa
//
LOCUS       DAELNY010000101        13611 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.101, whole genome shotgun sequence.
ACCESSION   DAELNY010000101 DAELNY010000000
VERSION     DAELNY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.101"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            222..2462
                     /locus_tag="KPE55_002383"
     CDS             222..2462
                     /locus_tag="KPE55_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0493443.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            2876..4411
                     /locus_tag="KPE55_002384"
     CDS             2876..4411
                     /locus_tag="KPE55_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0493444.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            4427..5188
                     /locus_tag="KPE55_002385"
     CDS             4427..5188
                     /locus_tag="KPE55_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493445.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            5526..6395
                     /locus_tag="KPE55_002386"
     CDS             5526..6395
                     /locus_tag="KPE55_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0493446.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            6724..6975
                     /locus_tag="KPE55_002387"
     CDS             6724..6975
                     /locus_tag="KPE55_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG0493447.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            7087..7866
                     /locus_tag="KPE55_002388"
     CDS             7087..7866
                     /locus_tag="KPE55_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0493448.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(8001..8651)
                     /locus_tag="KPE55_002389"
     CDS             complement(8001..8651)
                     /locus_tag="KPE55_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493449.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(8648..9151)
                     /locus_tag="KPE55_002390"
     CDS             complement(8648..9151)
                     /locus_tag="KPE55_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0493450.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(9200..9715)
                     /locus_tag="KPE55_002391"
     CDS             complement(9200..9715)
                     /locus_tag="KPE55_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0493451.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            9995..10519
                     /locus_tag="KPE55_002392"
     CDS             9995..10519
                     /locus_tag="KPE55_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0493452.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            10503..11906
                     /locus_tag="KPE55_002393"
     CDS             10503..11906
                     /locus_tag="KPE55_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0493453.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(12470..12781)
                     /locus_tag="KPE55_002394"
     CDS             complement(12470..12781)
                     /locus_tag="KPE55_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493454.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(12837..13430)
                     /locus_tag="KPE55_002395"
     CDS             complement(12837..13430)
                     /locus_tag="KPE55_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0493455.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         5098 a         1553 c         2291 g         4669 t
ORIGIN      
        1 ggtagaatag atgcaattac gcttaaataa aataactgta ttttttataa aaatcaaacc
       61 ctacactata ctaccagata aaatctacat gctataattt gacctgtgtt tataaaaaaa
      121 ggagtaatct aataaaatag tatttcactt atgtataact aacaactatt attacgattt
      181 tctctaaata atttaaagaa aaataatgca ggaggtaaaa tatgaacaat gaatttataa
      241 ttttaaaagg ttgcaaagag aacaatttga acaatatttc cttaaaaata ccaaaaagaa
      301 aaataaccat attcacaggg gtgtcgggtt ctggaaagtc atctattgta tttgagacaa
      361 ttgcaaaaga atcacaacgc caactaaatg aaaggtttag tacatttgta agaagttttt
      421 tacctaagta tggagaggtt aaagctgatt gtatagaaaa tttgtcaaca ccaattatca
      481 tagaccaaag tagacttggt ggaaattcaa ggtcaaccct aggtactatt acagatgtaa
      541 attccttttt aagagcttta tattcaaggt ttggtagtga gtatataggt aatgctaata
      601 tgttttcttt taataatatt gatggtatgt gcccacactg tcatgggctt ggaaaaaagc
      661 tagttcctaa tatgaaccaa attcttgata tgaataaatc attaaatgaa ggtgctatat
      721 tgctttcagg gtttggagtg ggttcttggc attggaatat attcgctgaa tcaggatttt
      781 ttgatattaa taagaggata tgtgattatt cagaagtaga attggaaaag tttttgcatg
      841 gtaaagcaga aaagattaag atagaaaatg ctggtcaaac taacatgaca tatgagggat
      901 taatggttaa atttaatcgc ctatacttaa gtagagaggg agaaatatct gaagctacta
      961 agaaaaaatt gagtaaactt cttatagaag atagatgtcc tatatgtgat ggaagaagac
     1021 ttaatgaaag agtatatagg tctttaatta atggttataa tatagcagat ttaactagta
     1081 tgcaaattga tgaattggct gaagttatta aaagtataga tgagccagaa gcacaacctt
     1141 taattaaagg aataatagaa aagttaaaca gtatcataga tataggtctt ggatatttaa
     1201 cactggatag agagacatct tctctatcag gaggggaatc tcagcgtata aagatggtta
     1261 agtatttaaa tagtaatctt gttgatttaa tgtatatttt tgatgaacca agtgtaggac
     1321 ttcatccaaa agatgtgtat aaattaaata atcttttaaa aaaattgcgt gataaaggga
     1381 atacaattat agttgttgaa catgacccag atgttattaa gattgcagac catattgtag
     1441 atgtaggacc aaaggcaggt aagtatggtg gagaagttgt gtatgaaggc agttatgaaa
     1501 atctacttac aagtggtact ttgacaggga atgctttaag taaatttctt cctattaaag
     1561 aaagtgtaag agaacatagt ggatatttag aagttaaaaa ttgtaataaa aacaatttaa
     1621 aaaatgtatc gattaagata cctaaaggtg tattgactct agttactgga gtagcaggag
     1681 caggtaaaag tacgttgatt aaggatgaat ttttaaagca aaatcctagt gctgtactta
     1741 tagaccaaag ccctgtttca gcaaattcaa ggtcgagttt ggcaacatat agtggaatta
     1801 tgaacaacat aagaagcata ttttcaaaaa caaatggcgt aaatgcttct ttattcagtt
     1861 caaattcaga aggagcttgt gataactgta agggttctgg aatagtagaa atgaatcttg
     1921 cttttatgga gagtataaag agtacctgca atgtttgtga aggtaaaaaa tttaaaaaag
     1981 aagttttaga atataggttt caagggaaga atataattga agttcttgaa atgtctgttt
     2041 tagaggcaat tgagtttttt aatttaaaac agattaaaac gaaattacaa tgtattgaaa
     2101 aaatgggtat tggatacctt actttaggac aaactcttga tactttgtct ggaggagagt
     2161 gtcaaagatt aaagcttgct agtgaattaa ataaggaaag ttcagtgtat atattagatg
     2221 aaccaacaac aggtcttcat atggctgata ttgagaattt tataagtata attgaagaga
     2281 ttgtagataa tggaaatacg gtcataataa ttgaacacaa tatagatgta attaaaagag
     2341 ctgattggat tattgagctg ggacctgaag gaggaacaaa aggcgggaga gtaatatttg
     2401 aaggtattcc aaaacaatta tgtaacaata aaatatcatt aacagcaaaa tatatagtat
     2461 aataaataaa atataataaa attataatga tattttaata aatataatgg gtatataaaa
     2521 tgataattaa acttaacagt aataaataat gtgagaaata ttcaaattta attaaataaa
     2581 gtgtcagaaa tttgtttaaa ttttagtatt ttaacggttc tgaccttttt tcttattttt
     2641 atggagaata tcgaggatgg agtctctaat agttactttt ttgtgtctac ttgcaagttt
     2701 gattaaaaaa tggctataca cacttgtgac ttcagtcatg ggaggttcag attataatct
     2761 taatatataa tttgtgaaat aatttgatga cgatttgctg atgtataaac aataaagatt
     2821 aactacatta aaatttaaat cttgttaagt aaaacttata aaaggagaga gatggatgat
     2881 aaatgatatt ctaaccttat tatctgaaag aagtgatttt tttataaagc tatccataga
     2941 acatttgatg atttctatta tttctataat aattgcgatt gttttgggtg gtataattgg
     3001 tattattgta agtgaatttc ataattcttc taaacctata ttgggaacta tcaattttct
     3061 atacaccatt ccatctattt ctatgctagg atttctgata ccattttccg gaattggaaa
     3121 tactactgct gttattgcac ttaccattta tgcattattg ccaatggtta gaaatacata
     3181 tactggaatt actaatgtgg ataaaaatat tcttgaagca gctaggggaa tgggaagtac
     3241 agattttcag atattgtata aaataaaact tccgttagcc ctacctgtta ttatgtctgg
     3301 tattagaaat atggtgatta tgacaattgc attagctggt atcgcttcat ttataggtgc
     3361 tggaggtcta ggtgtagcca tatatagagg aatcacgaca aataacacaa taatgacaat
     3421 tactggaagt atacttattg ctctattagc tttaatattt gattttattt tagggctggt
     3481 agaaaaattt tttcaacgaa aaagaaaaaa gaaaaatagg aaaaaagttt ttgccattct
     3541 aggaagcctt attcttatag caactatctt tacgtcttat ttatttaatc cttcaaagac
     3601 tcaaactatt catattgcta ctaaacctat gactgaacaa tatattcttg gtgagatgtt
     3661 ggatatttta attgaaaaaa acacaggtct aaacgtggaa attacacaag gtgtaggagg
     3721 gggaacatct aatatccagc cagctatgga gaatggagaa tttgacattt atccagagta
     3781 tacaggtaca ggctggaata tggtacttaa gaaaaatgaa ttatatactg aaagtatgtt
     3841 tagtaaacta cagtcggaat acaaaactaa acttaaaatg caatggactg gtatgtatgg
     3901 tttttctaat agctatgcat tagcagttag aaaagaagtt gcagataaat acaatttaaa
     3961 aaatataacg gacttaaaag atgtatcttc taaattgaca tttggagctg aatatgactt
     4021 ttatgagaga gaggatggat atactccatt gtgtaataca tatggactga actttaaaga
     4081 tacagttgat ttagatattg gattaaaata ccaagctatc aatgaaggta agattgacgt
     4141 aatgcctata tttacaactg atggtcagta tagtgtttca gatgttgtag ttctaaaaga
     4201 tgataaaaat ttctacccat cttatcaatg tggaaatgtt gttcgtttgg aagtattaga
     4261 ggaaaatcca gaactatcaa aggtatttaa gatggttgga gatattttga ctgataaaga
     4321 aatggctaaa atgaattatg acgtagaaaa caacaattta gaacctagag aagttgctcg
     4381 tgagtttctg aataagaaag gtcttttata aattggagga aatataatga cacctattat
     4441 acaatttaaa aatattaaaa aacaatataa tgataaaact ataatagata atcttaattt
     4501 agatatagaa aaaggagagt ttttgactgt aattggttcg tctggttctg gtaagacaac
     4561 actcttaaaa atgattaatg gtctgatttt gccagatgga ggaaatattt tgataaacca
     4621 aacagatatc aaaaacgagg acttaattaa attaagaaga agaataggat attgtgtaca
     4681 gggaagtgtt ctttttcctc atatgactgt tgaagaaaat atttcatatg taccaaatct
     4741 cctaagtaaa aagaataaat tagaagtaaa aagtgctgta aacaagtgga tggaaatagt
     4801 gggattgcca aatgatatga aagtgcgtta cccatcagaa ttgtcaggtg gtcagcaaca
     4861 acgtgtaggg attgcaagag ctttagcttc ttctccagaa attttattaa tggatgaacc
     4921 ttttggtgca gttgatgaga ttacacgaaa gcaattacaa aaagagataa aagaaattca
     4981 taaaaaaaca ggagttacaa ttatctttat tacgcatgat atttatgaag ctttgatttt
     5041 gggaacaaag actttggtat taaatcatgg agttgttcaa cagtatgata ctcctgagaa
     5101 tattttaaat actcctgcta accaatttgt agaccagtta ttgaatataa gaaaatctat
     5161 aatagatgat gataatttaa aagaataaat ttaaatttaa aatcaaattg ttgtgataaa
     5221 aactattgat ttagagctaa cttcaagttg tatacttgaa gtggaaaagt tagattaagg
     5281 tatagaaggg catttagcag ttggctgtag acgaggtaac tgctgaaggt gctttcaata
     5341 aatggctaga acctgtatct gttgaagatt ataataaatt gaaataagaa gctcaatata
     5401 cagagtagat aggcacttca aatagaagtg tcttttgtgt gccataaaat aaatcaaaat
     5461 attagcaaag aatgtgaaaa acaaaataaa ataatatgct atctttaaaa taggagggat
     5521 gctatatgga taacaatttg attaccattg aaaaaacttt agattatata gaaaaacatc
     5581 ttttagatga gatgttagat ttaaatgtaa tttctaaaga gataggctat tcaaaatacc
     5641 atttacatag aatgtttaca tcagttgtcg gatttagtgt acataattat atacaaagac
     5701 gtagacttac tgaagcagcg agaatgctaa tttttacaga aaaaccaatt attgagattg
     5761 ctttaatctc ggggtatgat acacaacgct ctttttcaag agggtttaaa aaaatgttta
     5821 aacgtagtcc taattttttt agaagacatc aagatttttt accactacaa ttaaaatttg
     5881 atattcataa tagaaaaaaa ttacgtggag atatggtttt agatgttaaa aaagttaatt
     5941 gtgcagatat aaatattgtt ggctattctg gtaaagtaag aaaaggattt catattattg
     6001 gaaaatgctg gagggatttg cataaaaata aagagatgat agaaaataag gtagatttag
     6061 gttttttaat tggagttagt gattatagca attttaaaga gattgagaat actcctacat
     6121 ttacatacat tgcaggtgct caagtagcta cttttggcaa attacctaat ggaatggaaa
     6181 aattcgtatt accgaaaagt aattatattg tttttacttt tattgggaaa aatgaggata
     6241 gtatgcaaaa tgttgtagaa tatatctatc aagaatggtt ccctaattca agttgccagt
     6301 ttaatgaaaa taatttatat gactttgtaa aatatggaga aaaaactgat gagaatggtt
     6361 tgagtgaaat tcagttttgg gttccaatca tttaaaagaa ggtattatgt ataaagtaga
     6421 tggatttata taggcaaagt tttacagcag tatgtattta taaataattt atagtttgta
     6481 ggaaagaaat aattttacta taagattatg ataaaataca caatactatg acaaaatagt
     6541 gttaaataat agatttaatt ttgcatagga aaagttctat aatggtgtgt attcataaat
     6601 aatttataat ctgtaggaaa gaaatagttt tactataaga ttatgataaa atacacaata
     6661 ctatgataaa atagtactaa ataatagatt taattttgca aaggataaag tgttttaata
     6721 aaaatgaatg tagaaaacga attacttaaa aacctagata gattgcatac aacagagtta
     6781 ggcgttgtaa gaattaagaa aaatctttct ttggagacaa atgatgtagt gaactggtgt
     6841 aaaactaaaa tagaatctcc aaatgccatt attaatagaa agggaaaaaa ctggtatata
     6901 agtgtatatg attgtattat aacagtaaac gctcacagtt atacaattat tacagctcat
     6961 aaagaaaaga aatgaattgc aaaatacaaa ggtcaaaagc attttccact gtttgtagta
     7021 cctatgaaga agtatataaa ataatattac ttttgaataa taatttaaat tttggaggtt
     7081 gaaaaaatga atgatactgt aaaagaaaaa ttggcagagt cactaacttc tgatactact
     7141 gatattttac cttatttgcc ttatttatta caagattttt gggaattagg aagttcacca
     7201 gaacaaatgt tacaattatt tagaaagcat atacctttta atgaggatac aaaagttttg
     7261 gatttagctt gtggaaaagg tgctgtatct gtgaagcttg caaaagagtt gaatatacat
     7321 atcaaaggta ttgatttgat acctgaattt attgattatg ctagaagaaa ggctattgaa
     7381 tataaggttg atgctttgtg tgattttgaa atgaatgatg ctaatagttc tgtaaaaatt
     7441 gagcatgatt atgattgtgt gatttttgga gctgtagggg atattttagg taattataaa
     7501 gaaactataa gtaagctatt acctacaata aaaagtaagg ggttcatttt aattgatgat
     7561 gcatatgtag tagataaatc tacaaatgaa cagcttaatt ttgagagggt gtatcccact
     7621 tacaatgagt ggtgtatatt gttcaaagaa ttaggtctga agctaattga atgtatacca
     7681 tgtttagaac ttgaaaatga tgttgaagag gaaatgaatt ggattataac tcgtgcaaat
     7741 gagctaatta aaaaatatcc ttctcaaaaa gatatgtttg aacaatatgt tattagccaa
     7801 aaaaatgaat atactgattt acagcaggac cttgttggag caacttggat tttacaaaaa
     7861 gaataagata aatattagac tagataatca atgttatagt gaattatccc atgtctagtg
     7921 aatataaata acataaaaaa ccgtcaagat gaatagttta ttcgtgcttg acggttttta
     7981 gtatttgttg aagtaaaatt ttatttcttt agtatatcac ttttagtcaa tccatttgat
     8041 ttagcttgag tattccattg atgattcccc ataagtttat ataggaatct agggaagtta
     8101 ggtgaaataa aaatagtact tttgttggaa tcatctatat gtatattatc actaagtata
     8161 ttttttgaaa gttcttttaa tgcaattcct aaatcttttt taggtccttt acccaaaggt
     8221 acggattcta gtgaaccaaa catctcccca gctccaattc caataccttg aacccatgta
     8281 aagttgattt ttttacacca atgctctatc atttgaattg ccaaatgatt atgttcacct
     8341 tcattgaatc cacagttaac tattgcatac actggaatag gttttttatt ttgtgttttt
     8401 aaaaattttt cacatttctc taaaaaatga agtaaatgag atggaatacc atctacataa
     8461 agaggagatg cgattattaa tctatcacaa ttaagtatgg cattaagagc gtcaggatac
     8521 ttagatgtgt tatctacttt aaatgtaaaa actttattat cattttgaat aagttgtttt
     8581 aattcagata aaaaatactg tgaattattt ttatttactt ttggactacc atttatcatt
     8641 actatattca tatggattca cctcttatat cttgtatatt ttcatagaaa caaacagagt
     8701 gttcttttat attgaaattt atagcatttc catttactaa tttcgtagca gtttcttttt
     8761 cagcttctgt aatacaatct ccatagaata tagcttttaa atttatctgt ttatcatatc
     8821 tgcttttatg acgcatcata ttatctttta ttacaaagta tggaagaata taattaatgc
     8881 ttttatcaag tacgtttttg acaaaagggc taaaccctcc gtaacagcat ctgcttatta
     8941 taataattcc atcagagtca tttataagtt tacccatgtt gctgtaataa tcttttacaa
     9001 cacattcaga aggagtttta atccagcaac caaaacaacc aatacatttg tggatttctt
     9061 ttgtgtttgg aagtattttt atattgtcat caagtttggg taataacttt tctaattctt
     9121 tgtcagttaa atcatgtatt aaaagcctca taaagtccat ctccttttat tatgtatgtt
     9181 tacttctaaa aattatatct tatagatttt tcagtaaatc tttacaccaa ttaataacaa
     9241 aattataata gtcaactcca taaatcattg ttgcatacat atatgtatat tggtcaacac
     9301 aatgattttc ttctgcatca tttttttgtt tacttaattt atttagaagt aattctactt
     9361 cagttattaa catttttaag tttgtttcaa ttatttcttt gggtagatac ttgtaaaaga
     9421 atatattaac aaggtggtct tgctttgttt tagaaaaatt tataggtttc tttaaccatt
     9481 caagaaatac ttctttacca gtattagtta tggaatatag tttttttaat ttgttaccat
     9541 ctataacttc gtgatagtgt atatatcctt ttgtttctaa tcttttaagt gcaggataga
     9601 tacttccaaa gctggcgtca aaaaagtacg aggtactttc tgacattatt tgttttaagt
     9661 cgtatccact taattctttt tccattaaaa atcctaaaat aatgtattct aacatattac
     9721 ctcctggtat caattagata tatatcgatt cgatatattt attattacag ataataaatc
     9781 ttacgtcaag atgcttttta agataagcta aaaagaatta ggagttattg ataaatatgc
     9841 aaaaaaatta atataaaagg taattaaata tcaataaaga tttaaaaata gttaaatcaa
     9901 agtaagagtc taatatataa aatttatagt aactttatgg tttaaatata aacttgtgtg
     9961 ctattgacaa agtataaaat tagcagtaga ataattgcat gagcaaatag attgttgcga
    10021 acgcaaaaaa aggacaatag atattaaagg agagaatatg agagagctat taaaatgggt
    10081 ttccattata aatagataca atacaaatta tattgacagg aaattggcgg atattggaat
    10141 caataatagc caatattttt atgtattaca catatgtaat aatccaggta ttacacagga
    10201 cactatattt gagaaaattc ttgtgaatcc tagtaatata acaagagctt tggcaaattt
    10261 agaaaaagaa ggctttataa caagagaacc aggtgtaaag gataaaagaa catggcattt
    10321 atatcctaca gaaaaatctt taggatgtta tgatgaaata ctaaaaataa caaatgggta
    10381 tgttgaggag ttgttgagtt catttgagaa agaagagcaa gagttgttta tgtcaatgtt
    10441 aaagaaagtt gcatttttgg ctattgatat gaatgaagaa gcaaaattgg aggcgaagga
    10501 aaatggaaga aaaatataag gagccattgg aatatgaaaa ggtttcaaag ttattaacta
    10561 agtacgcaat tcctagtatt atagcaatgt tagtaagtgc tttatataac attgttgacc
    10621 aaatatttat tgggcaaggt gttggtgtat tgggcaatgc tgctacaaat gtcacttttc
    10681 cactaacaac aatatgtact gcaattgcat tattgttagg aattggagga gcgtctaagt
    10741 gttcattgga attaggtgct aaaaattcag aaaaagctac aaaagcagca ggaaattcta
    10801 tatgcttgat ggctgttttt ggaataagtt tatttatagt agtatcaata tttttaacac
    10861 cactattaaa attctttggc tctacatcag aaatactacc ttatgcacac acatatacaa
    10921 aaattacatc aattggatta ccatttctta taatgtccac tggaatgagt aagttgatac
    10981 ttgcagatgg gagaccgaaa gcttctatgt tttgtatgtt aataggagca gttattaata
    11041 caattttgaa tccattattc atttttgttt ttaatatggg aattgctggg tctgctttag
    11101 caactgttat tgggcaactt atatcttttg gaataagtgt tcattatata aagaattttc
    11161 aacatattaa attacataag aaaagtttta aattagatag tataatttat aaacatatat
    11221 ttgcgctagg ttcaagctca tgttttaatc aattagcaat gactgtaaca cagatagtta
    11281 tgaataatac attggcatat tatggggcta ggtcagttta tggaagtgag attccacttg
    11341 cttgtgtcgg tatcattatt aaagtaaata tgatttttat atctataatt attggtatat
    11401 cacaaggtat gcaaccaatt atagggtata attatggggc tgaaaagtat aatcgtgtaa
    11461 aagaagcata taagtttgca gtgggaagtg caactattat atctgttatt gcattcttat
    11521 gtttccaatt attcccaaga caaattacgt ctatatttgg taacggaaat gagacttatt
    11581 ttatatttgc agaacattat tttagaactt tcttgttctt aacatttata aatggtgttc
    11641 aaatagtatc atcaactttc tttacatcaa ttgggaaatc agtgttggga ctttttactt
    11701 ctttgactcg tcaaatttta tttttaattc cattgatagt aattttacca atgtttatgg
    11761 gaattgatgg agtaatgttt gcaggaccaa tagcagatgc agcagcggca atggtatgta
    11821 tatactttgc agttcatgaa atgaaatcat tgactataaa gcaaaataat ttgatgagta
    11881 accataataa gattgcagag gtataagaca atatttaaga agtatagaaa attaatttaa
    11941 agaagtataa taaactaatt taggaagtat agaaaattaa tctaaagaag tataataaac
    12001 taatttagaa agtatagaaa attaatctaa agaagtgtaa taaactaatt taggaagtat
    12061 agaaaattaa tctaaagaag tgtaataaac taatttagga agtatagaaa attaatttaa
    12121 agaagtataa taaactaatt taggaagtat agaaaattaa tttaaagaag tataataaac
    12181 taattcagga agtatagaaa gctaatctaa agaagtatag taaactaatt taggaagtat
    12241 agaaaattaa tctaaagaag tataataaac taatttaaga aatatagaaa attaatttaa
    12301 agaaatacaa tacattaatt tttaagtaac attaaattaa tgtaagtaaa ataatgttca
    12361 tatgaagatt taaaggtgca ttttctgaag ctaagtatat gttgtagagt gttaggtctc
    12421 tctatcctgt gaagtttaat agctcaagaa aatgcactat ttatgatgat tattttgttt
    12481 tatttccaat tttggaaata actattctaa ttaaatctaa aattatacca taaataagaa
    12541 aagaaagtat agatgctatg taccaataaa tcgaaattga ccctgtaatt gttctacatg
    12601 ggatttgcat acctgaatcc ataaaaacaa aaaaactatc aactctggaa ataatagaca
    12661 aaataggatt taaacctatt agaacaatac tttttgagtc ttgaccaatt tgatgcatat
    12721 aaatctcaaa taatgcaatt aaaaaaaacc aaaatgtaaa agatttaata atttttttca
    12781 tcaaaatacc tcacataata taaattctgt ttgcactatt tgcttactac aactatttat
    12841 ctccattcag tcacatcact taattgtaat ctaggtcttc ttgatggttc ttcatcagga
    12901 taacccaaag atattgctgc cactaattga ctatctgtat ttaaccattc acataattca
    12961 cgatatgcaa agtatacatc acaaatccaa agactaccta gacctaattc cacagctgta
    13021 agcgacatat tctgaattgc agctccaatt gattgcatgt ttgccatttc atagaatcgt
    13081 tcttcagggg atagcttttc taaaggtgat ttacctaata tatttaaaat aaaaattgtt
    13141 actggagctt gtttcatgat ttcaactgtg taatttgcac cagcgatatg ttgacgactt
    13201 cctggaagaa gcccattatc attaatttca ttttgaatac ctttgctcat ggctttaagc
    13261 atagattctt tttccttttc agtgatgaca acaaaacgcc atggttgcct gtttttagat
    13321 gaaggtgcat ttattccagc ttcaattatt ttctctatag tttcatgtga aatgctttga
    13381 tttttatatt ttctgatact tcgtcttttt gatatagaat cacttatcat tttaagtaac
    13441 ctccgtttat tttttggaaa aatctattta atttttattt tctattttaa ttattttagc
    13501 attaatcaga aaaaaagtca ttgattttag tatacaatta ttaatgatta tattaagaat
    13561 tgtatcaaaa agtatgtgat tatactaagg gattatatta aacattatat t
//
LOCUS       DAELNY010000102        13538 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.102, whole genome shotgun sequence.
ACCESSION   DAELNY010000102 DAELNY010000000
VERSION     DAELNY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.102"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..452
                     /locus_tag="KPE55_002396"
     CDS             6..452
                     /locus_tag="KPE55_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0493456.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            454..1548
                     /locus_tag="KPE55_002397"
     CDS             454..1548
                     /locus_tag="KPE55_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG0493457.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1636..2166
                     /locus_tag="KPE55_002398"
     CDS             1636..2166
                     /locus_tag="KPE55_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0493458.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(3101..4477)
                     /locus_tag="KPE55_002399"
     CDS             complement(3101..4477)
                     /locus_tag="KPE55_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493459.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4746..5918
                     /locus_tag="KPE55_002400"
     CDS             4746..5918
                     /locus_tag="KPE55_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0493460.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVNSMFERVKLISTVILNLDKFEV"
     gene            5932..6579
                     /locus_tag="KPE55_002401"
     CDS             5932..6579
                     /locus_tag="KPE55_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493461.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6581..7090
                     /locus_tag="KPE55_002402"
     CDS             6581..7090
                     /locus_tag="KPE55_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0493462.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7454..8566)
                     /locus_tag="KPE55_002403"
     CDS             complement(7454..8566)
                     /locus_tag="KPE55_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0493463.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8864..9130
                     /locus_tag="KPE55_002404"
     CDS             8864..9130
                     /locus_tag="KPE55_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG0493464.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9375..11102
                     /locus_tag="KPE55_002405"
     CDS             9375..11102
                     /locus_tag="KPE55_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493465.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11102..12892
                     /locus_tag="KPE55_002406"
     CDS             11102..12892
                     /locus_tag="KPE55_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493466.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13054..13362)
                     /locus_tag="KPE55_002407"
     CDS             complement(13054..13362)
                     /locus_tag="KPE55_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493467.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
BASE COUNT         5076 a         1565 c         2232 g         4665 t
ORIGIN      
        1 ttaagatgga ttttaaaaat ttacagtatg aaagtttgtc atatattatt tgtaacttgc
       61 aaaaaaactt taaactttat tgtgaaaaat gtttaaaacc atataaactt acgaatggtc
      121 tatatttcta tttaatttat attaataaaa ataggaattg ttctttgaat gatgtttcta
      181 cagaatttga agttgataaa gctcatacaa caagaactat atctaggtta gaacaagatg
      241 gatacataga aaaaatccaa aaccctaatg atagtagagc ttttcagttg agagtaacag
      301 ataagggtga ggaagtatta ggggatataa aaaacatatt ttcaaagtgg gataatcaca
      361 taaaaaaaga gttctcggat acagaatata aagaactggt aaagaattta catgtggtaa
      421 aggatataaa aacagctgtg gaggaagaat aggatgtatt gtaatttatt aaaaccaatt
      481 aaaattggaa atttggaatt aaaaaataga gtatcttttg cacctacaag catgggtctt
      541 aaattggaag aaaaaattaa aaagttttct gatattgcta agtcaggagt agctctgatt
      601 actctaggag atgtatctat tagacctagt tttcataagg tagctataag cctatcggat
      661 gaagatggag tcattaaata caagaaaatt gtagatgaaa tacacaattc tggtgcaaag
      721 gtatcagcac aactattttg ttcagattat gatgttaatt taattaaaga tactatgaat
      781 atgggaatta cttcacatga tgagataaaa aaaataatga atgacggagt aaaagattac
      841 ataacaaata tgcccaaaga agaaattaaa aatattataa atttatttaa agtaacagca
      901 ttgaatgcaa aaaaagcagg ttttgacatg attcaaattc atggtgatag gcttgtagga
      961 agtttttcat caagtatatt taataataga aatgatgaat atggtggaac gtgtgataat
     1021 agaagtagat ttgcaagcga aataattagt agtatccgag atgaagttaa ggacattcca
     1081 atagactata aatttgcaat aagacaagaa aatcctcatt atggaaatgc aggagtttta
     1141 ctttcggaag ttgagtattt tgttaaaaaa tttgaatccc taggtgtaaa cagttttcat
     1201 gtaacacttg caaatcattc aaaattagaa gatactatac caaccaataa tcatccttac
     1261 tttaaagatg aaggctgttt tttatattta gctgatgagg taaagaaaca cacaaatctt
     1321 ccagtatgtg gggttggaaa attgtctagc ccagatttta tagaaagtat tatttcaaat
     1381 aatagagtag atatggtttc tatgtcaaga caattactag cagatagtaa ttggttacag
     1441 aaagtaaaag atggaagagt agatgagatt aaaaagtgct gttattgtaa taagaagtgt
     1501 gcagatgctt tacaaacacg ctctcagttt ggatgtatac tagattagaa tatccttata
     1561 aaaataaata atttgttaag gttaatatat ttttggtgat ttagataaat atttagataa
     1621 agaggaggag acatcatgaa aagtttagtt ttatattcaa gtttaactgg aaatactaaa
     1681 aagattgctt atactatata tgatgaaatt caagaagaaa aagacattaa agatgtaaat
     1741 gaattagttg attatggtat tgactatgaa aattatgata tagtattctt aggctattgg
     1801 gtggacaagg gaatttgtga taaaaattca aaacaagtgc ttgaaaatat tcacaataaa
     1861 aagatagcac tatttggaac aatgggagca agtaaaaaag gaagttatgg agctagtata
     1921 atagaaaaga tagaatcaat tatcccaaaa gataatgaaa tactaggttc attcatatgt
     1981 caaggtaaga tagcagaagg attgaaagca aaatataaag agatgttaaa actttctcct
     2041 gacaacgaac atattcgcca acaattaaat aaccatgagg aatctcaaag tcatccagat
     2101 gaacaagaaa tatatgaagc cagtatgttt gcaaaaaata tgatgattaa agctagtatt
     2161 gtgtagtagt taaacaataa gttcatataa gatatagaat aaataattta gtaaaaaagt
     2221 atagcaaata atttgctatg ctttttttat gaaatcaaaa aaataataaa aatacttgac
     2281 tgtaaacctt gtttatacat tattttatag aaaataagat agttttaaat ataaaaaaag
     2341 atatattatt gaaaggagat attattgtat gaaaacttat aaaagataat tagtttgatt
     2401 ttaagatata ctaaatatac aattttatag agtgaaaata tttaagacta tattatagtt
     2461 ataaattaat agaatagaga ggtagtgttt aaattgcaat aacagtattt ccaaagttgc
     2521 cacctcaaat tcctatccaa tggaataatg gtgtagctaa ttatgtagat aaaaaattta
     2581 tttatgccta ccctgtcatt tgtgttgtat tcagaatcct tttacgtcca tttatatgga
     2641 gatggttaca aatacatgct tttatagtga tactattgca gattatttaa cgaattttat
     2701 gtgttttgta gcgttgtcta tagaaatatt ctctatattt tttatttaca atattgccag
     2761 acatgttaca ataattattt ttatagatat acttgtattt attgctttac tgataatagg
     2821 tgtgtataaa ttatttaaaa aatacaatta atttttaaaa ataagtaata agtgatagtg
     2881 taaaaagtag ccactgcaaa ttatggattg tagtggctat tttattattt gtatatgaaa
     2941 ttattttgtt tttctaataa gttacgatat attatggttt atttcactga taaaaggttt
     3001 aggtatttta aagtaaaaag ttgtatattc taataaaatt taaacatttt gattctttaa
     3061 aattagagaa aatacattgc ttttattaaa aaattaaata ttaagaattt aatttctgca
     3121 tcttatttag aaaagataag aaatcaactt tatatatttt ttttggcctt cctctttgat
     3181 tatcgagttt tgtatttaca atagttgcaa ttcctgcttt atataagata ctcaatattc
     3241 tattagcact tctaagagtt atgttcatat agtctgctaa tatttctgct gacacattat
     3301 ttgaatttct atcattaatc atacacatga ttttagatac atttgtatta gatagaggta
     3361 taaaagaagc aactttattg gctattgagc tatcttcaat attattagaa tttttatttg
     3421 aatataaagg acctattgta tcattagtat catttactat aaaagtacag tttccattca
     3481 aagaagcaga tttaccatta gcttgacgag cattttgctc tgcatgaata tttgtatttc
     3541 caatgcccca acctatattt aagctatatt taatttcttt taaatcttct gaaataaaaa
     3601 aatctgtata gttattggtc atattgttta acatttcttt agtagtgtgt atctctattt
     3661 tcatatcatt ttttttgacc tgaactccat agaatccatg tcttgataga gaatttaata
     3721 tagtgttata tatttgattt tgtatctcat catttttaaa tatcaaatct tcatttatgt
     3781 actctgaact atttactgta actatacaag ttgcagattt atttttgtct aagttttgta
     3841 atgttatttc attcaccaaa gatttaaatg tattcaggat tgattctgta gatggaacca
     3901 tgtaaataca atcatatcca agcttattaa gttcacaact aatgatgtaa tttctagtaa
     3961 aagaaaggtt aatcttatta tctcgcaata agtctaaatg tatttccaat aatttatcat
     4021 ttaatttttt aaagtcatat acaggttgag ggatattaaa atctttaatt gtatatggac
     4081 atttattaga gtctattaat gaatatatat cataataatt gttgtcctct cttaaaaaat
     4141 ccataaatga ttttgtaaag tcaatatctt tttggctatt taataatttg aataagtatt
     4201 tataaaaatc tctttcatca agctttaaaa aatttaatgg aacttttatg tcttttatat
     4261 ttattataag ttcactatat ggaactatac cactgcatac aaatccatca aaattatcta
     4321 cttgctcata gtatatatta ggtaaattat taaaaggatt ctccccttta agctcaataa
     4381 atgtaatttc acaagtatca tttaattctt taagagcttg tttaaactta tctgacataa
     4441 aataagatgt tgtcaaaaaa ccaattttat atttcataaa acctccttta aaatttcgag
     4501 agtattgtaa ctaatcaatc aatttataac ctcattttat aatagtagat aaaacgcgtc
     4561 aatttatttt acaaaaggaa aattgaaaga aaaatcagaa aatataaaaa aagactgaaa
     4621 aatatgttgg ctgtcaagaa taattgtgct attttgtaat taaggactag tcttttaaga
     4681 tttcctaaaa taaacttaga ttttaggaaa gaaatacatt ttatttgaaa ataaaggaga
     4741 agattatgaa tacatattta aatgaaattt ctaagtttgt tgatgagaat agagaagaaa
     4801 tagtttcatt atggaaagag attgttaata tggaaagcta tacacattgt aaagaaagcg
     4861 taaataaatt ggctaagaga ttaaagtttg agtttgaaaa agaaggttta gattgcgagt
     4921 tagtagatgt tggagataat ggtagtacat tgataggtac acttggttcc aatatagata
     4981 aaaagccaat aattttttca ggtcacatgg atactgtctt tgaaacaggt acatttggag
     5041 agaatccatt taaaataata gaaggaaaag catatggccc aggtgtttta gatatgaagg
     5101 gtggtataat aatttcctta tatgttatta aagctttaaa taaaatagga tacaaagaaa
     5161 gaccaataaa aatagtattt tctggagatg aagagatagg acataaggat tcaacaggtg
     5221 cagatgttat attgagggaa gcaaaaggtg gtctttgtgc ttttaatatg gaaactggat
     5281 tagtagataa tagtttgtgt gtaggtagaa aaggaagaat aggatgtaat atacatgtaa
     5341 aaggtgtaga aacacatgca ggtaatgatt ttgaaggtgg aagaaatgct atagaagaaa
     5401 tggctaataa gatactacgc atacaaaaat tgactaattt agatgttgga actacagtaa
     5461 gtgtaagtgt gattaagggt ggaagagtgg aaaattctat tccagaagat tgtagtataa
     5521 aagtagacct tagatttgaa aaagtagaag aaatggagaa tgttaaagaa caagttagag
     5581 agatatgtaa agaaacttac atagaaggaa cttctacaga agttaatttt ataagtgaaa
     5641 tgatgccatt tgaaactaca gaagatgtta tgagatttca tacatttgta aatgaagtgt
     5701 caaaggaaaa tggatttgga gaattaaatg ctaagagact tggaggtagt tcagatgcaa
     5761 gttatttaac tattgcaaac gttcctacaa tctgttcatt tggtgtaaga ggggaatgga
     5821 accacacatc aagagaatat gcagtagtaa attctatgtt tgagagggtg aaattaattt
     5881 ctacagttat tttaaactta gataaatttg aggtataaag ggggatttta tatggatgta
     5941 acagctggag ataataaggt aacatggaaa gggtatttat ctttatttgt attgatagta
     6001 ttattttcag gagtgtttaa agatagtcaa ggcttcctta aagcattaga ttttgctaat
     6061 ctgacaggtt catttggtac tattgcagat ggtgtagact ttatgggtaa aggaggaacg
     6121 ggagcaaaag atggtttctt atttgctctg actttgattc caactgtctg ttttgctgta
     6181 ggtcttatag atgtagttga atctatggga ggaatgaaag cggcatcaaa gatatttaat
     6241 ccattgctta gacctctgct tggtatacct ggaatagcag gaatagcatt tgtatcttca
     6301 tttactagtt cagatgtagc ctcaataatg acgaaagaat tatttgaaag tggggaaata
     6361 acagatgatg aaagaactat atttgtagca tatcaatatg ctggttcagc tgttatactg
     6421 aacacaataa atactcaagc tccactttta ccaatagcat tattggcatt aggtccaata
     6481 atccttatag agatattctg taaaatactt ggtgctaatc tagttagatt aatcataggt
     6541 gtaagaaata aaaagaataa ggctaaggag gctgtgtagt atgagtcaac aaaatactgg
     6601 aaatgcaatt gtaaatagta aacctaagaa aactgctgtt gagatattta tggggggagc
     6661 aaaaaaaggt ttttacatag gtgttgaaca gatattacca gccatgataa tgggttatgt
     6721 aatagtgcag tttttacaat taactggttt agttgatatt ttaggaaata tttttggacc
     6781 agtaatgggt atttttggac tacctgggga atctgttgta gttttgatag cagcattctt
     6841 tgctaaggca gcaggagcag ctacagctgc aaacttattt gcacaaggtc taatcactgc
     6901 accacaagca accatattaa ttatgccatg tatgttaatg ggtactctag ttggtcatta
     6961 tgccagaata gtgcttgtag caaatgccag tactaagtat agaatgttac tgttggcagt
     7021 tcctatattt gatgcaatag tggggatgtt aatagtaaga gttttactca ctgttatggg
     7081 attaatgtaa ataacataaa attataacct aactataaat gatatataag aattaatata
     7141 tacaaggatt aatgagtagt aattgatata tagtaataaa ctaagatagt aattaatgtg
     7201 tatattaatt aaaaaaagag agtatctcaa agtgaacttt ttagttcata agatactctt
     7261 tttgtatgaa atcaagtata ttttcataat ttcaacaatg aaagagaggc ttatctctat
     7321 tttgagacag cctcttcttt taaatttata tttctagaat ttatgttttt tatttatcca
     7381 acattaacat tagttattag aaataatttt aagaaaaata tactttcatg ttaatatcag
     7441 aataaatgtg tttttaaagg atgatttgtt ttgaattttc acgaatatct acttctatga
     7501 ctttttctat tgtatctcct ctaaaaccta ataaattact tgtcaagttt gcagtggaac
     7561 aagaatgatt aggtataatt cttattctat caccaatttt tatatcagtt tcaccattta
     7621 ctttaatttt tccaacttct tctgaaagtg aatcaatagt aagttctgga tgtccacata
     7681 ccctaccata accaacaata ctactatttc catgtgcacc tttatcaagt ccaaggcact
     7741 tacttccaca atcaattaaa tagtatccac tgctattttt tgagattaca gtgcacacaa
     7801 cagtaagagc acacatatct tcagtagcac aacctaaacc tatctgtata gcatcatagt
     7861 atacatagtt tccaggtctt acagcagtta tagttttaga agcagcttca gattcaactg
     7921 ttggagtact accagaagcg acaatttcta tattaaatcc attatcagtt agagctttgt
     7981 atgctttctc catagaagaa tcttcttgaa cacaaacatc tggaacacct tctggagaac
     8041 tacaaccata cacttgacct ggatgcgaac ttataccaac aatcttgatg ttttctagct
     8101 tagaaatttc agtagcaagt tcaaccactt tttctggttc aaccccaaaa cggtgtaagc
     8161 cacaatctac aataataagt agcttgcact ctatttttga tttagaaaaa gcttcactat
     8221 aagccttagc acattctata ttatctgtag cacagattaa atttattttt gatgccatag
     8281 tcacaattct atttatattt acttctccca tgtaaggata agccaacata atatcttcaa
     8341 aacctgcttt tatcagagct tcaccttcat caagtgttcc aactagaaaa ccactagcac
     8401 ctgcattttt ttgaagtctc gctatgtaag tacttttatg tgtcttaagc ataggccaaa
     8461 gttgtttatt attatttgta caaatttgtt gaattttatc tatattcttt tctaactggt
     8521 ctaaatctag caaaaaattt ggagtagcga cttctgaaat tttcatgtta tttaccacct
     8581 ttcaacaaaa gtaaattaac tataagtatt ataattatta tattagtaat atcggcttta
     8641 taatattaat agcttataat tatattatag aactagcgtt aacagagagc aatatttatt
     8701 tgtaataatt agaattttca ataagttagt gtagttttaa attatttata taatatattt
     8761 ttaattttat gcaaagttta tttaaattag tttgaggatt tattaatagg gtattattga
     8821 atgagtaaaa aagtgatgtt tacatagaat aatcacacta taaattaatt tagaacctaa
     8881 aaaattaatt gagcaaattg gaaaagagaa ggataaaaaa gatatttgga tagttggtgg
     8941 aggcgatata gtaaaattat ttatgaaaga taacttaatt gatgattatt atatatatat
     9001 attgccaata atattgggga gagggatttc actatttaag tctggtatag acaagactaa
     9061 tttgaatttt aagaaagcta gtaatattgg agaacttgta aaattggaat acagtaaaaa
     9121 acaaaaataa attacatgcc aatttgttac aatgttatga aacgaagcat ttaaaagact
     9181 tttggctata tgaattgaca ttacttgtca aatatgttaa catataagcg acttgtcgga
     9241 gggtgtatta aaaagaatat gctggagaag agagtgcgtt aactcttttc tccttttttt
     9301 ataaaattaa gcatttataa atttagtatt attacatgat tacactatct caaaaattaa
     9361 gaaaggagaa aaatatgaaa ttaattttaa gttatttaaa gaattataag ttattaatag
     9421 tattaaacat actggctata tttagttttg cattagtcga gttggggatt ccaactataa
     9481 tagctaaaat aattgacaat ggtattgcaa atcagaatat agcttacata aaacaaatgg
     9541 gaatagtgat tgttattatt tctattatag gggttgtagg tagtatattg ttgggttatt
     9601 gttctgctaa ggtatcaaca agtgtaacca gagatataag aaatgacata tttgagaaat
     9661 cacaagaatt ttcacatact gaatacaata aatttggaat ttcttcaatg attacaagaa
     9721 caacgaatga tgcatttcaa attcaacaat ttgttaatat actactaagg actgccttat
     9781 taactccagt tatgttcatt ataagtataa taatgacaat tagaaccagt gtggaacttt
     9841 ctatagtatt ggctatatca gtacctttta taattattgg agtagcaata atagcaaaag
     9901 tatctcaggc aatatcttca aaacaacaaa aaggactaga taaattaaac cttatatcga
     9961 gagaaaattt aactggaata agagtaatta gagcttttgg aaatgatgat tatgaaacag
    10021 agcgatttga aaaaacaaat acatattatg ctaatgtttc taagaaacta tttaagctta
    10081 tgtcaattac tcaaccagca ttctttttac tgttaaatat agctgttttg gctgtatttt
    10141 ggatttcaag tgagaagata aatattggag agcttcaagt tggacagttg gttgcatttt
    10201 tggaatatct tttccatgcc atgttttcaa ttatgctttt ttcaatggta tttattatgt
    10261 atccaagagc agaggtatct gcaaatagaa taaaagaact attaaatgaa gaaccattga
    10321 taaaaaatcc tgaaaatgga attaaggata ctgaaaataa gggtatcata gaatttgata
    10381 atgtaacttt tacctatcca gatggagaag cttctgtact taaggatatt tcatttactg
    10441 caaaaacagg tgaaacagtc gcttttatag gaagtactgg tagtgggaaa agtactctaa
    10501 ttaacttgat acctagattt tatgatgtaa cagaaggtag cattaagata aatggagtag
    10561 atataagaga atatgattta aaagctttaa gaaaaaagat aggattcata cctcaaaaat
    10621 cactgctttt tactggtagc atagcaaata atattagatt tgggaaacat aaggcaggag
    10681 agtcagaatt agaatattcc gcaaaagttg cacaagcata tgagtttata tcaaaaaaac
    10741 ctagaaagtt tgatgaattg attagtgaag gtggagcaaa tgtatcagga ggtcaaaaac
    10801 aaagactttc tattgcaagg gctattataa gaagacctga aatatacatt tttgatgaca
    10861 gtttttctgc acttgacttt aaaactgatg caatacttcg tgctaaattg aagaaggaaa
    10921 caaaagatgc aattgtattg attgttgccc agagaattag ttctattatt gatgctgata
    10981 agattatagt actcaatgaa ggacaagttg taggaatggg aactcataaa gaattattaa
    11041 aaaactgtga aatatactat gaaattgcaa catctcagtt aaaaaaggag gaattagaat
    11101 aatgaaaaag tctaaaggtt ttaagaaaac aattggtaga cttcttccat ttgtaaagaa
    11161 atataaattt tcctttatta tagctattat atgtataatt tcagcagcta ctatgaatgc
    11221 acttgcacct aaaactgaag gattgataat aacacaattg actaaagatg taattagtat
    11281 cgcaaaagga gtaccagggg cttccgtaaa ttttgattat gtaacaaaaa tattggtgat
    11341 tttagcatgt atatattttg ccaatgcaat ttttacgtat acttcaagtt ttctacttac
    11401 gaatgctatt caaaacacaa tgagagattt aagaaatgaa gtcgaaaata aaataagaag
    11461 attgcctatt agttattttg atagtaatag ttttggagat gtattaagtc gtatatcaaa
    11521 tgatgtagat acaatatcaa atgcattgca acagagtttt atgcaaattg taaactcaat
    11581 cttagtcata attttggctc tttcaatgat gtttacaatt aatatatata tggctcttat
    11641 agctcttttt ataattccaa ttagttattt tgtatctaaa tttgtagtaa aaaaatctca
    11701 aagtagattt tcattgcaac aaaatgctct tggaaaatta aatggaaaag tacaagaaat
    11761 gtatactgga tttaatgaaa taaaactata tggtaaagaa gaagattcta taaaagaatt
    11821 taaaaaagta aatcaagaac tttgtgaaaa tggatttaag gcacaattta tatcaagtat
    11881 gatgaatcct atggtttcat tagtaactta ctttggaatt gcagctgtgg cagttgtcgg
    11941 ttctatatat gcagtaagtg gaggaataac agtaggaaat ttacaagcat ttgtgcgtta
    12001 tatatggcag ataaatcagc cactttcaca aatgactcag ttatctacag taatacaatc
    12061 ttcttttgca gctatagaac gtgtatttga aattttagac gaagaagagg aaatacctga
    12121 tgttgaaaat ccagttaaga tagaaaatgt aaaaggaaat gttacatttg agcatgttaa
    12181 ttttggatat ggagaaaatt caactttaat agaagattta aatgcagaag taaaaagtgg
    12241 tcagatggta gctattgttg gacctactgg agcaggtaaa acaacattaa taaacttatt
    12301 gatgaggttt tatgatgtaa agaaaggtgc tattaaaatt gatggagtag atataagaga
    12361 tatgaaacgt aaagatttgc gttcaatgtt tggtatggtg cttcaagata catggttatt
    12421 taatggaacc atttttgaaa atatagaata tggtagattt ggggctacta aagaagaaat
    12481 tatacaagca gcaaaagtag caaatgttca tcactttata accactcttc cagatggata
    12541 caatatgttt ttaaatgaag aagcatctaa tatatctcta ggggaaaaac agcttttaac
    12601 catagctaga gcttttatat cagacccttc aatattgata cttgatgaag ctacgagttc
    12661 tgttgatact aggctggaac ttatgcttca aaaagcaatg agaaatctta tgaatggtag
    12721 aactagtttt gttatagcac atagactttc tacaattcgc aatgctgact tgattttagt
    12781 tatgaataat ggaagtatta tagagcaggg gaatcatgat gaattaatgg aaaagggtgg
    12841 attttatgag aagctttata atagccaatt tgcagacaaa gaatcagaat agataacata
    12901 aacttttgta taaaattttg tatcaaattc aaatattaat catataatta aaaagagtat
    12961 cagtaagttg tttttttata gatttactga tactctttga ttttaagact aaaggtattt
    13021 atgattggta cttataattc acaactagtc ttgttacatc tgataaccag aattacagta
    13081 cttttctttt ttaccttttt gttctctaac atataacaat tcgcttaact tatcaatgta
    13141 ataaccatca tcacttatgc gaattaaact agtcagataa tatccatctt tgcttctagg
    13201 aatcttttta tttattgcta aatcaatagc tgtctttgct gcttcaaaat gctttaaatg
    13261 tgtatgacct tcttcaaaac tctttctagt attataaact acataacctt ttttaacttg
    13321 aaaaactata aattctttct tttcatatat tttattaaac attcaatcag cccttctcaa
    13381 gatagtttta tagcataaca ttagaagctt ctaaaaaatt ttacaaagaa cataaatatt
    13441 ttataaatta taaatctaaa actataattt ataaagataa ctttctgatt atttatatga
    13501 taattcatat agattaaaag taaattatct ttaggtta
//
LOCUS       DAELNY010000103        13517 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.103, whole genome shotgun sequence.
ACCESSION   DAELNY010000103 DAELNY010000000
VERSION     DAELNY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.103"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..1427
                     /locus_tag="KPE55_002408"
                     /pseudo
     CDS             1..1427
                     /locus_tag="KPE55_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1600..2793)
                     /locus_tag="KPE55_002409"
     CDS             complement(1600..2793)
                     /locus_tag="KPE55_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0493468.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQVLLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSK
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFANHKGKPKVAIDYTALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2872..3075)
                     /locus_tag="KPE55_002410"
     CDS             complement(2872..3075)
                     /locus_tag="KPE55_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0493469.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(3075..4166)
                     /locus_tag="KPE55_002411"
     CDS             complement(3075..4166)
                     /locus_tag="KPE55_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0493470.1"
                     /translation="MTTHKNLSVKIDYISIVFETATAEDVIMHILGLPTDIFNVYPAS
                     IKYKTYQARWQIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNYTFGD
                     MFRRCERRYGLDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDES
                     KFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKHNKTLEEVGSWKRTEMQLRDDKAH
                     AFAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAVEVLKL
                     QVPKQQNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSNSLSS
                     KLTAHLQRINRTDLIPYIQYDTKHGKGGI"
     gene            4632..5303
                     /locus_tag="KPE55_002412"
     CDS             4632..5303
                     /locus_tag="KPE55_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493471.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNYPTFDTLEKIMDVLGVTPNELLSGEWKYIDHTEPYIMDIIKREQDFNVSLDYLS
                     ENEFFDDEKECTFYKAYKLIQYIHNYITNEVTELEELMEIKQLIQRQKLERMIKVHKE
                     MRGLDRYREQPKEYKYHDPYDDWIFRQLADIDNRNNIPDTSPQVDFNEGDYEDYLKAK
                     WNRGL"
     gene            5614..6093
                     /locus_tag="KPE55_002413"
     CDS             5614..6093
                     /locus_tag="KPE55_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBG0493472.1"
                     /translation="MANEDKKDFNAMLHDSKDMPKFQIIIDQKSIEKYGGNKMYFAPP
                     IDYDKVMKKVPYGKVITVGKIREHFAKLSGADFTEPITAGIFVSIVAWASYQRSEDET
                     PYWRTLKANGELNAKYPNGIEAQKEKLEAEGHTIIQKGRKNIRYYVKDYENSLFDLK"
     gene            6445..6861
                     /locus_tag="KPE55_002414"
     CDS             6445..6861
                     /locus_tag="KPE55_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG0493473.1"
                     /translation="MKTIKCKLTVYFAEPFWVGVFERICDGKLSVAKVTFGAEPKDYD
                     IQEFVLKHYYDLQFSPAVETIVKKDKKNPKRVLRDVKKQLQNNGIGTKSQQALKLQHE
                     LKKEEYKSNSKHKKQMDAEQKFKLKQQKKKAKHRGH"
     gene            7104..7499
                     /locus_tag="KPE55_002415"
     CDS             7104..7499
                     /locus_tag="KPE55_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011068146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HBG0493474.1"
                     /translation="MAISNINTIISNDIQKVWNIVLAVDKYNSWRSDLSKTEIINDKQ
                     FIEYTKNGYATTFTVTVVEPYKRWEFDMDNSNMKGHWIGIFTDKGNETQIDFTENVIP
                     KKWFMKPFVKTYLKKQQKQFVLDLKKALE"
     gene            7526..7846
                     /locus_tag="KPE55_002416"
     CDS             7526..7846
                     /locus_tag="KPE55_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HBG0493475.1"
                     /translation="MASSKDYLQFVLEQLSELQEITYRAMMGEFIIYYRGKIVGGIYD
                     DRLLVKPTKSAISYMPTVTYEIPYENAKEMLLVEEVDNRDFLTGLFDVMYDELPTPKP
                     KKKK"
     gene            8081..8572
                     /locus_tag="KPE55_002417"
                     /pseudo
     CDS             8081..8572
                     /locus_tag="KPE55_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
     gene            8833..10032
                     /locus_tag="KPE55_002418"
     CDS             8833..10032
                     /locus_tag="KPE55_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG0493476.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            10084..10779
                     /locus_tag="KPE55_002419"
     CDS             10084..10779
                     /locus_tag="KPE55_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0493477.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            10796..11473
                     /locus_tag="KPE55_002420"
     CDS             10796..11473
                     /locus_tag="KPE55_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493478.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            11584..13185
                     /locus_tag="KPE55_002421"
     CDS             11584..13185
                     /locus_tag="KPE55_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0493479.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         4822 a         1843 c         2582 g         4270 t
ORIGIN      
        1 ttgaactgtc aagctatgag cttgtagtat gttcagacaa tgatataaag gaatatatac
       61 ataataatat tgataaaaat atacctattg taatagtaca tcgtactata aatattgaaa
      121 atataaatca gataataagt atagaaaatg attctgatgt tatggttata gatgcttata
      181 aagagtcagc agatgaaact gccaagataa ttagaaaact aggattaatt catattaatc
      241 taataccata ttatccaggt tgtgataagt ctaaatgtga gattggaatt attacaggaa
      301 gcagaaacag cataccacaa aacataaagc aaataattga cataggtgat aaggtaattg
      361 atataaatac agttatagaa atctttacaa aactaaatat atctatagat aaattacata
      421 ttattaagga aaaatacaat gaagatacag taagtggata taggtattat acaactatga
      481 ataaaaccat gaagagtttt ttggagatta tagatgaagg tattgcttct gttgataaat
      541 taggaaaatt tatatattgc aacaaagtat tttctaatct agttggaata gaccaaaatg
      601 aaattatatc taataacttt atggacttat ttagtgataa ggttgtcaaa aaaatatttt
      661 ttcaagaaga tgaagttaat gatgaagtag taaatttaaa taacaaaaaa ctgataatta
      721 ataaagtaaa tgtttatgaa aataatgaac gaataaagag tattataagc attaaaaata
      781 taagtgcaat tcaagtgctt gaggacaaga ttcaaaataa acttcagtca aaaggttttg
      841 tatcaaagta tacatttgag agtgttgtag gtgagagtaa aataataaaa gaaaaaatta
      901 atattgcaag aaaaatagca actacagact tttctgtgtt gatacttgga gaaaatggaa
      961 caggaaaaga aatatttgcc caggcaatac ataatgaatc tctaagaaaa aacaagccat
     1021 ttgttgcagt taatctatct tctttgtcag atacacttat tgaaagtgaa ctttttggct
     1081 atgatgaagg aagttttaca ggagcaataa aaggtgggaa aatgggtata tttgaaagag
     1141 cacacacagg aacaattttt ttagatgaga taggtgatat atctttagat gtacagcaga
     1201 gattgctaag agtattacag gaaaaagaag ttatgagaat gggcggaagt aaaattatac
     1261 caatagatgt tagaattata gctgcaacaa ataaagattt aaagaaaaaa attaaagaag
     1321 gttcctttag agaagatttg tattatagga ttaatgtatt acatatagag atacctagac
     1381 ttagagaacg taaggaagat atacctctta taagtaagta ttttttgtta taaattatat
     1441 atgtcttttt gactatcttt ttatatcttc tcaaatgtcc gcaaagcctt attttatagg
     1501 ctctacgggc attttgcttt tgtggtaaac ctcgcatatc taggtctatc ttcttatatt
     1561 ttcgctatca agcgtggtta aaatcgtggt aaatacttct tatgcaatta gacgctgaac
     1621 ctctgatttt gcggtatcta tggacgcatg agcgtaccag ttcattgtga tactgatatt
     1681 tgaatgcccc atgatatact gtaaatcttt cgggttcatg ttcttgcttg ccagtcttgt
     1741 gcagaacgta tggcgtagtg tatgcggtgt gatatgtggc aagggattgt ccttatggtg
     1801 cttgttgtat ttctttacca tgcggataaa taaggcggta taatcaatgg caactttggg
     1861 cttgccttta tgattggcaa ataagaagtt gctccgtccg tctatcacaa atggttctgc
     1921 ctttggacgt ttcttcatca ctcgttgaaa tgcttgtatc gtttccttgc ttaatggtac
     1981 ttgccttgtt ccgctctttg ttttgggcgt ttcaatataa tagccttgtt ccttgctctt
     2041 taataactgg tggtcgataa tcacaacttc attcttgaaa tcaatatcgg ctactgtcag
     2101 tccgcacagt tccgagatac gaagtccagt tttcaacagt atcagcacat catcataata
     2161 tttgtgatac acgttgtccg tcttgatgaa tgacagtagg acttgttcct gttcctctgt
     2221 caatgcaact ttctctttgg tatcattttc taggacttca cttaacttaa aatcaaaagg
     2281 gttcttcctt acacaatcgt cttgtatggc gatatagaat gacgctttta acgagcgttt
     2341 atggttgtta atggtattgt aggaaaagcc tttctctttc atgcgtaacg cccattcctt
     2401 agcgtcagag ggttttatcg tatcaatgct cctagcaccc aacttgtcct ctttcaataa
     2461 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
     2521 ctgtttggcg tagagttggc aaagtgtcat tttcttgcct gtgctgtcga taccgtcctc
     2581 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
     2641 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tatttcgtgt ttccaaatgc
     2701 gtccacatat ttatataagt attttccgtc tgttcgttgg ctctctccag tatgcaggat
     2761 acgtcctttg ctatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
     2821 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagattgc
     2881 gtccaattta tcaatgacct tttcaaactg tcggcgttta atctgtatgc ggttgccatt
     2941 cataatgagc cagccagcgt ccttgttttc ctctgccaag cgtcttaact tgttttctcc
     3001 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
     3061 atcgttgtta ttcatcagat cccccctttc ccatgtttcg tgtcatattg aatataaggg
     3121 ataaggtcgg tgcggtttat cctctgtaaa tgagcagtta gtttactgga aagggaatta
     3181 ctgtatcttg ccttatcaag catagttccc actttttcca gtccacctaa tgcgtcatgt
     3241 tcttccaaga agtaaaaact tttgacagcg gaaatcacgc caccctctgt aagccattgc
     3301 tgtgtttctt caagggagtt ttgctgtttt ggtacttgca gtttcaagac ttccacagcc
     3361 cctaaaaatc gttcccaaaa ccgacacgtt ttccatctgc tcttattact ttcgtttctg
     3421 tttggcacga caaagcgtag gttatttgcc aatagtccga aagccagttc cccaagttcc
     3481 agcggtctgt ccttgaatgt catggcaaaa gcatgagcct tatcatcacg cagttgcatt
     3541 tctgtccgtt tccaactgcc aacttcctca agtgtcttgt tatgttttga acagacttcc
     3601 ttatccttat cataaaagcg gtaggacaat cccgatttcc cagcaccgat ataaacagtc
     3661 tttgcggtgt cgaaatcatc aaacttgctt tcgtcaaagt ggtagccctc actattcgag
     3721 ataaattcct ctttttcaca tttcttcttt atctgctcta tggtaaaaaa tggcttttcg
     3781 ttcttatcat caatggcaat atcaagcctt gtgaaatgga agttatccag tccgtatctt
     3841 cgctcacagc gccggaacat atccccaaag gtataattcc tactgtcgag aatacggaaa
     3901 atatcatcac aacctcttcc ggtcataaca agataacagc ctaacccttg tgggttgtcc
     3961 tctgtctttc ttgcgtcccc cgatacataa atatctccaa tctgccagcg tgcttgatag
     4021 gttttgtatt taatgcttgc cggatagaca ttgaaaatat cagtcggtaa acctaaaatg
     4081 tgcatgataa catcttcggc ggttgccgtt tcaaatacaa tactgatgta atcaatctta
     4141 acggataaat ttttgtgtgt agttatagtg catttctcct ttcgtacttc tcgttttttt
     4201 gcggggtaaa aattgtgttt tgtcctttat ttatcacagt ttctaggtac tatgacatgt
     4261 atgcgcaggg cggtgttaca tatacgccct ttacgaacgc ctaaaggcgt tccctttctt
     4321 cctgtgtctg ccccgttctt aacgctcacg agccttgtaa ggctcgtggc taaacggaac
     4381 agccccagtc agaaaccttg ttttcggtct gatagtccat gcttattcat gctatcaaag
     4441 tgaaaataca tatttttgtg cttactcaag cgtgtgattg accctagcgt gcttaatcac
     4501 tctatctgat gcgataataa cgccattaga attacttgtc aagaagtttt tgttgacttt
     4561 tagatatata ttaagtatga tatgcttata aataccaaga aaagcaacga aaggagcggt
     4621 tatactaagt tatggagcat tatgaaaaga aaatcagttt cttaggagag aacatacaga
     4681 ccataagaaa acacagagga atgaaacaac aggaacttgc ggacaaaatc ggtatcaata
     4741 tgcagagcct ttccaaaatt gaacgaggtg tgaattatcc tacctttgat acgttagaga
     4801 agataatgga cgtgctgggt gtaacgccca atgaattatt gtcgggagaa tggaagtata
     4861 ttgaccacac tgagccctat atcatggata ttatcaaacg ggaacaagac ttcaatgttt
     4921 ccttagatta cctgtctgaa aatgaatttt tcgatgatga aaaagaatgc acattttaca
     4981 aggcatataa actcatacag tatatccata actacattac caatgaggtt acggagttgg
     5041 aagaacttat ggaaatcaag cagttgatac agcgtcaaaa actggaacgc atgataaaag
     5101 tacataagga aatgcgaggg ttagaccgat acagagaaca gcccaaagag tataaatacc
     5161 atgaccctta tgatgattgg atatttcgtc agcttgcgga tatagacaac agaaacaaca
     5221 ttcccgacac ttctccacaa gtagacttca atgagggaga ttatgaagat tatctaaagg
     5281 cgaaatggaa cagaggtctt taatttcctc ttgcatggaa gatacagcaa acaaaaagcc
     5341 cagtaaagag tgcaaagcac caccaatggt ggcaaggctc ttgacagggc tttttctttt
     5401 ctgttgtggg gtaatcaaga ggaagaaaga aaaagtgtgc atttttgttc cataaatgct
     5461 aagggtatgc tgatttttat tgtggcggat atatggaact gttataatat ggatatgaaa
     5521 aaagcgacaa atttgaaagt gttaataatt ttcaaacagc ctcattgcat ggaaataaga
     5581 aacagactat ccttaaagta ggaggtggca caaatggcaa atgaagataa aaaagatttt
     5641 aatgctatgt tgcatgatag taaggatatg ccaaaatttc aaattatcat agaccagaaa
     5701 agtattgaga aatatggtgg aaacaaaatg tattttgctc ccccgattga ctatgacaaa
     5761 gtaatgaaaa aagttccgta tggtaaagtg attacggttg gaaaaatacg agaacacttt
     5821 gcaaaattga gtggtgcaga ttttacagag ccgattacag cgggtatatt tgtttctatt
     5881 gtagcgtggg cgagttatca gcgttccgaa gatgaaacac cctattggag aacattaaaa
     5941 gcaaacggag aattaaatgc taaatatcca aatggtattg aagcgcagaa agaaaaatta
     6001 gaagcagagg gacataccat tattcaaaag gggcgtaaaa atatacgata ctatgtaaag
     6061 gactatgaaa attctctttt tgatttgaaa taggcaaatt ctatttcaca atttcatatc
     6121 tctatacaca aagcagttag tcttaggatt gactgctttt ttcataccta aatttcagat
     6181 tggagtgatg aaatggtgtt tgttagagct gttcaataag attatcggtg cataaaattt
     6241 tattgtagtg ctgatatagg aatggtataa ttttcaagaa aggtaatcaa ataatgatta
     6301 tctaaaatga caaagataaa aacgcaaagc attttattat gtgagagctg gtaggtagcc
     6361 cagccgtcct atgtttatag ggtaagtaac ctgcccctcc gggttgtccg ttctttgttt
     6421 ggaaataaac aaggagggat tgatatgaag acaataaaat gtaaattgac tgtgtatttt
     6481 gcagaacctt tttgggttgg agtttttgaa agaatttgtg atggaaaact ttccgtagca
     6541 aaggtaacat ttggtgcaga gccaaaagat tacgatattc aagaatttgt gttgaaacat
     6601 tattatgatt tacaatttag tccagcagtt gaaacgattg taaaaaagga taagaaaaat
     6661 cctaaaagag ttcttagaga tgtaaagaaa caattacaga acaatggaat tgggacaaaa
     6721 tcgcaacaag ctttaaaatt acaacatgaa ttaaaaaaag aagaatataa atctaattcc
     6781 aaacataaaa agcaaatgga cgcagaacaa aagtttaagt tgaagcaaca aaagaaaaaa
     6841 gcaaaacaca gaggacatta aatgatgttg caatatcata aactaaatat tttataagtt
     6901 catatctaca caaagcagtt agtcttatga ttgactgctt ttttcttgcc aaaatttcag
     6961 atcggagtga tgacatggtg tctgttagag ctgttcaata agattatcgg agcataaaat
     7021 tttattgtag tgctgatatg agaatgttat aatgtggata tgaaaaaacg ataaacggta
     7081 atttaaaaag gggaaatgaa aaagtggcaa tttcaaatat aaatacaatt attagtaatg
     7141 atattcaaaa agtttggaac attgttttag ctgttgataa atataactca tggcgaagtg
     7201 atttgagcaa aacagaaata ataaatgaca aacaatttat tgaatacaca aaaaatggat
     7261 atgctactac atttaccgtt accgttgtag aaccatataa acgctgggaa tttgatatgg
     7321 ataatagcaa catgaaaggt cactggattg gtatttttac agacaaaggg aatgaaacac
     7381 aaatagactt tacagaaaat gtgataccta aaaagtggtt tatgaaacct tttgtaaaaa
     7441 cctatttgaa aaaacaacaa aagcaatttg ttctggactt aaagaaagca ttggaataat
     7501 aaaattcaag attggagatt tagaaatggc ttcaagtaaa gattatttac aatttgtttt
     7561 agaacaatta tcagaattac aagaaataac atatcgtgca atgatgggag aatttattat
     7621 ctattatcgt ggaaaaatcg ttggcggtat atatgatgat aggttactgg taaaaccaac
     7681 aaagtctgca atttcttata tgccaacagt tacttatgaa attccatacg aaaacgccaa
     7741 agaaatgtta ttggtggaag aagttgataa tagagatttt ctgacggggt tatttgatgt
     7801 gatgtatgat gaactaccaa cgccaaaacc taaaaagaaa aaataaacag cttctagttt
     7861 acaatttcat atccatatac acaaagcagt tagttttagg attgactgct ttttaaaaag
     7921 acaccattta ggtgtaatga tgtcttttgg gtgttatttt ataggtcata tcaaggctca
     7981 ttcaatcgtg gtaaattcgt ggtaaaatga gttttttcta tacttcaaaa tacttgaaag
     8041 tatagtgttt atgcggataa tcgaaaatta gatataaaat ttagatgaaa taaatagcaa
     8101 caaatgtttt acagaggaat caatgcaagc tttaacattg tatgaatggc caggaaatgt
     8161 aagagagctt aaaaatttaa tttattatat tgatactata gttgaggaag atagagtaga
     8221 ttacgagcat ttgccagaac aatttagatt tgaaaaaaac aatactttag ttaatgaaaa
     8281 tttcgattca attatcttag atttcaaaca atctaatttc tttgaggaaa gtatatgtat
     8341 attgaccagt atagaaactt ggaataataa aaatatatta ttaggaagaa ataaactgca
     8401 agagatttta aaagaaaaag atatagtttt aagtgtagac caaattagaa aaagaataga
     8461 taagttaaag agccatggtt tacttttatc tggagtaaaa aaacaaggta gttttataac
     8521 tgatgaagga aagaacttta tatcatatgt aaaaattaag ggagtgatat aggggactaa
     8581 aaaggcaaaa caccctaaaa aaatccctat aatggataga aataaataaa attatcttta
     8641 agattaagat aattcaaaaa tattgaataa ataatctaag aagattattt ttaataaaac
     8701 agtagtaaat atcaacctta ctactgtttt tattttttgc aagatttatt atacaattaa
     8761 aatttatatt ttggcacatc aattgctaca taggaaaaat ataaaaaatt gtaaaacttg
     8821 gaggatgttg ttatgaagtc agcttacaaa ggatatgaag cctatagtta tttagaaaaa
     8881 ggaaaagata tttcatctat tgagatgtgt aaaggagata aaagagttaa agaatacctt
     8941 attgaattag atgatattca agaagctaag gttaaaagta tagtggatga aaaaatattt
     9001 atatcattac atgaacatcc agtattattt ccagaacacc tagaaagaga tatatttgaa
     9061 tataataggg aaggtaaaca aagatgtgct tatgaagcat tatcaagagg atattatgat
     9121 gctatatttg ataatttaat ggatggtgta tgtacgataa catcaaattc agggtggaaa
     9181 tgggatgata tattaataga tttaggcatg agattatgtg atttagctca tcaagatttt
     9241 gtcataaagt gtgaaaaagt agaagatata tacagagcta aaagtgaagg taaagtagca
     9301 ttgataccaa cattagaagg tgcagcacca atagaaaatg aacttgatag aatagatata
     9361 ctgtatggat ttggtataag gttaatgggt ataacataca gcgaatccaa tgcattaggt
     9421 tcaggactaa aagaagagaa agatggaggt cttacaaatt ttgggaaaaa agctgttgaa
     9481 agaatgaata aaattggaat ggcaatagat tgctctcacg ttggagttca aactacactt
     9541 gatgtaatag aatatagcaa ggacccaata atattaagcc atgttggggc aagaaattta
     9601 tggaacacaa agagactaac tccagatgaa gtattaatag catgtgctaa aaaaggaggg
     9661 gttataggtg tagaagcagc acctcatact acaataacag agaagaataa agaacattca
     9721 atagagtcat ttatggaaca ttttgagtat attaaaaatt tagtaggaat agaccatgta
     9781 gcttttggac cagatacagt atatggcgac catgtaaaat tacactcagt actagctgct
     9841 aaatttgata taaaaaaaat aacaggaaca aaagaacatc ccaaagtaga atatgtaaaa
     9901 ggtcttgaga acccaacaga aacttcaaaa aacattgtta gatatttagt aaagcatgga
     9961 tatagtgatg aggaaataga gaaggtacta ggtggaaata tacttagagt actaaaacag
    10021 gtttggaaat agatatatta gaaatattaa taaaatttga ggatttatag ggaggtttta
    10081 taaatggatt atttaaaatt agctaatcat ccacttttgt ggatagcgag tacagtagca
    10141 gtatcaatag tagtggtaca atcccttata tttgctataa aatcttgcaa agttggtaaa
    10201 acaatgggaa taacagataa acaaataaag tcagctgtta agagtagtgc tatttcagct
    10261 attgggcctt caatgactat atttgctgga atggtatcgc ttatagtaac tatgggtggg
    10321 cctatagcat ggatgagatt atcatttata ggttctgtaa tttttgaatc aatgtcagcc
    10381 ggatttggaa cagatgcatt aggtataact ttaggttctc ctgaaatgac aaaacttgca
    10441 tttacaaatg cagtttggac tatgatatta ggttctttag gatggataat attcacatta
    10501 ctatttggac ataaactaga taagataacc aatgttatat ctagtggcaa gaaatcattt
    10561 ataccaataa tatctctagg agcaatgttg ggttcatttg catatctaaa tgctgatagg
    10621 gtgttgcgtt ttgataatgg aacaattgca tgtataagtg gaatggctat aatggtaata
    10681 ttgtgtatgc ttgaaaagaa gaaaaatata aaatggttaa gagagtgggg gcttacaata
    10741 tcaatgttta gcggaatgat aatagcttca gtaatataag gggggagtta gttttatgag
    10801 taaagtaaaa tctatataca atgaagaata tttacctttt atgataaggt atggaagatt
    10861 aacactatct ttgggtatta tagcagcatt agttcctgga ataattttga gctttggatt
    10921 tggtattatg cctccaatat cagcattatt agcaagtaca atggcaattg tcagtatgag
    10981 tgctccaaat tacataatag aaccagtttc ttattcacca atcttaggta taccaggaac
    11041 ttatatgagt tttctttctg gtaatatatc taatatgagg ctaccatgtt caatagcagc
    11101 tcaaaaggca gcagaagttg aatcaggtac agaagaaggc tcgataatat ctacaatagg
    11161 aatagctgta tctattttag tgaatataag tatactaact ataggggtaa ttttaggagg
    11221 aagcgtacta tctaaaattc cagctgaagt agtagaaaaa ttaaacttaa ttttaccagc
    11281 tctatttggg tcagtttttg gacaagtatt tttacaagat aaaaaattag gtctagtagc
    11341 aattgtaata tctgtattga ctattatatt atcaaagcaa ggtataatac cacagtctct
    11401 agtagttctt atatgtgtat ttggtactat attaattgca agagctatgt ataaagataa
    11461 gcttagtgac taattacaat gaaataatag tgaattttca aatatgaaat ttaataattt
    11521 gattttcaaa tataaaaatt acattaagat tatttaaaaa ttggttttta aggagggaat
    11581 attttgatat atgatttaat cttaaaaaat ggatttataa tagatggtac aggtaatcct
    11641 ggtttttatg gagatatagc aattaaagac aacttaatag caaaaataga ttctaagata
    11701 aatagcaata caaataaaga gattgattgt tgtggaaaag ttataacacc tggatttatt
    11761 gaccctcatg ttcatgaaga aatagtagca atactagatg gtaaatttga aaagtttcta
    11821 aaacaaggag ttacaacaac tataaatggt aattgtggac attctataac accatattct
    11881 tctgaaaatg tgtatgaata tatgtataaa aatggtctat tacttgaaga agaaaaaaag
    11941 tatcttatag ataaaaataa atgttggaat aattttacag aatattgtga ccttatatct
    12001 aaaagtggta tttctgtcaa tatgggattc ttgttggggc atgggactat aagatggagt
    12061 gttatgggag gttctaagga tagacctcct acagaaaagg aaaaaaatga aattacagat
    12121 ataataaatg atggaatgaa aagtggtgct tttggaatat ctactggatt ggcatatata
    12181 ccaagtaagt atgctgatat tgatgaactt gttgatattg caaggcagat taaagagtat
    12241 gatggtatat atacatctca tattagagat tacataggaa gatataatgc agtaaaagaa
    12301 gccatagagg ttgggcaaaa aagtggtgct agggttcaag tatctcactt aagtccagtt
    12361 gaaatagaag catttgatga gatattaaag gctaggtata atggtgttga aataatggtt
    12421 gacacagtac caagaagttc tgggcattgt atgaagaaaa aaagagtaat tcaatttatt
    12481 atggcaatat catctagtct atttgaatta ggtatagatg gggtaatgga tgctcttaga
    12541 aatgaagagg gaagaacact tatattaaaa gaagcattta tacttggaga tagggggagt
    12601 ataatacttc taaacactaa agatattaat atggaaaaga aatcaatacg tgaaatagct
    12661 acacaaaaag gaatagatga agacaagtta ttactcgatt tacttttaga tggagacgaa
    12721 gaacttatct tttgtttagg aggaatgtat agagctgatt tcccagataa actacatgat
    12781 gataaaataa ttgataatcc atttgttatg gttggttcag attgtttgtt tagtgtgggt
    12841 ggggatatgt catggtttga actacaaaga agaggagctt ttccaatttt cttcaatatg
    12901 tacagaaagt ctggtgttag attggaagag attgtaagaa gagttacttc attaccagct
    12961 aggcaattta aaataaaaaa tagaggtatt cttaaagaag ggttgatagc tgatatagca
    13021 gttattgaca tggataacta tagttatcct aggtcagaag atattgattt ttcaaagcca
    13081 caattactag ccaatggtgt tgaatatgta attgtaaatg gaaaaattgc attagaaaat
    13141 ggagagatta ctgagaataa atgtggtgaa gtattgaaaa gataaggttc taaaaataat
    13201 aggatttaga aagtaaataa atatctaagt aagtaaagac gaagtttgag atttatttaa
    13261 ataaaaatta tgcttcgttt ttatttatag tattaatatt tattttaata ctcagataat
    13321 atttatacaa aaaatattta tactttatag tgaaaaaatt aatattttta atcattaaat
    13381 tatgtaaaaa attggataaa tagataacta aatcatttga gaaaagattt ttagaaataa
    13441 aaaatgataa ctaaataacc cctttaatat ttatcaaata gccagtcata aatattaaaa
    13501 ggggttattt agtttat
//
LOCUS       DAELNY010000104        13500 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.104, whole genome shotgun sequence.
ACCESSION   DAELNY010000104 DAELNY010000000
VERSION     DAELNY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.104"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..1239
                     /locus_tag="KPE55_002422"
     CDS             163..1239
                     /locus_tag="KPE55_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0493480.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1356..2198)
                     /locus_tag="KPE55_002423"
     CDS             complement(1356..2198)
                     /locus_tag="KPE55_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0493481.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKNVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2382..2954
                     /locus_tag="KPE55_002424"
     CDS             2382..2954
                     /locus_tag="KPE55_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493482.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3117..3389)
                     /locus_tag="KPE55_002425"
     CDS             complement(3117..3389)
                     /locus_tag="KPE55_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493483.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDVHTHEHK"
     gene            complement(3516..4388)
                     /locus_tag="KPE55_002426"
     CDS             complement(3516..4388)
                     /locus_tag="KPE55_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0493484.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4684..7791)
                     /locus_tag="KPE55_002427"
     CDS             complement(4684..7791)
                     /locus_tag="KPE55_002427"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG0493485.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7884..8116)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8291..8818)
                     /locus_tag="KPE55_002428"
     CDS             complement(8291..8818)
                     /locus_tag="KPE55_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG0493486.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8853..9638)
                     /locus_tag="KPE55_002429"
     CDS             complement(8853..9638)
                     /locus_tag="KPE55_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG0493487.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9648..9905)
                     /locus_tag="KPE55_002430"
     CDS             complement(9648..9905)
                     /locus_tag="KPE55_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG0493488.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9924..10382)
                     /locus_tag="KPE55_002431"
     CDS             complement(9924..10382)
                     /locus_tag="KPE55_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG0493489.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10422..11126)
                     /locus_tag="KPE55_002432"
     CDS             complement(10422..11126)
                     /locus_tag="KPE55_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG0493490.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11136..11771)
                     /locus_tag="KPE55_002433"
     CDS             complement(11136..11771)
                     /locus_tag="KPE55_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493491.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12152..13192
                     /locus_tag="KPE55_002434"
     CDS             12152..13192
                     /locus_tag="KPE55_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0493492.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4314 a         2268 c         1592 g         5326 t
ORIGIN      
        1 aaagtcattt tacttccgta tttaatgcaa aagtagagat taaacttaat ataaaaaata
       61 tataacaaat tttttttata tttctagaag aaaagtatac tcttttttgt ttaataatgc
      121 taaaattgat ttgtattgaa ttttgattag gaggaaatta ttatgaaagc tcaaagatta
      181 aatgctgtac ttgaacaaat gaaaaaagat gatatttctc aaatgttagt aagtgaccct
      241 acttctatat tttatcttac aggagtacta atccatccag gcgaaagact attagcatta
      301 tacttaaatt taaatggcaa taataaatta tttataaatg aattattccc agtttcagaa
      361 gatttaggtg ttgaaatggt ttggtttaat gatactcaaa atccagtaga aataataact
      421 gaacatatag acaaaaatgc tactatgggt gttgataaaa actggccagc tagattctta
      481 ttaaacttaa tagaattagg tggaggaagt aagtttgtaa attcatctta tataatagat
      541 actttaagaa tgtgtaaaga tgaagaagaa aaagagttaa tgagaatagc ttcaaaatta
      601 aatgacaagg ctatggaaca acttaaagct actgtttctg gtgaacttac agaaaaacaa
      661 cttgttggta aattatctaa aatatatgaa gacctaggta ctgatggatt ttcatttgac
      721 cctattatag gttttggacc taatggagct aatccacatg gtgaacctgg aaatgcactt
      781 gtgaaaccag gagatgcaat aatcttagat ataggttgta taaaagataa ctattgtgct
      841 gatatgacaa gaactgtatt ctataaagaa atacctgaaa aaggtagaga aatatttgaa
      901 atagttcttg aagctaataa gagagctgaa gctattgtaa aaccaggtgt tagattctgt
      961 gatatagatg ctgctgctcg tgactacata actgaaaagg gatatggaca gtacttcact
     1021 catagaactg gtcactctat aggacttgaa gttcatgata aaggagatgt atcttctata
     1081 aatacagata ctgtacaacc aggaatgata ttctctatag agccaggtat atatttacca
     1141 ggtgagttcg gagttagaat agaagattta gtacttgtta ctgaagatgg ttgtgagata
     1201 ttaaacaagc atgacaaaga aatatgtgta gttggttaat tctaaaatca atttgataaa
     1261 attaaaaagc catataaaaa tgttttaaac atttttatat ggctttaatt tctcaatcta
     1321 ttatttattt caatccatta tttatattgc agtatttata ttgcagttat taagattgca
     1381 ccctttgaag tatatgtccc catcactgaa ccaatttcac agattacaac atctttgtat
     1441 tttacattct tttccataac ttcccttacc ttttgagcct tttctgggca attagcatgt
     1501 cctataaata aaaccttttc ttctttatct ttaatttttg actctactaa agtaatcagc
     1561 tttttcaaac tgtttccttc acctcttgcc ttatctatag gagttacaac gccatctgca
     1621 acttgtatta tcgccttaaa atttaaggta tttataattt ttcctgctat tggatttatt
     1681 ctaccaccct taatagcatt ttctaaagtt tcaagtgttc caaaaaatgg aggattatct
     1741 ttatatttat ttattattga aacaatctct tcaatttctt ttccttcttt tattaatatt
     1801 gctgctttta acactaattg tcctattcca attgacccac taagtgaatc tataacctca
     1861 actttcttgt tatttccttc cttttcaaac atatcttttg ctaaaagtgc actattataa
     1921 gtcccagata atcctgaact tatattaatc actaatacac tttcttcctg acaatggtat
     1981 gcttctaaaa atttatcagg agaaggacat gatgtttttg gtagtacttt actctcactc
     2041 atttttctat agaaagtttc attatctatt tcctttccag atatataact ttcttctcca
     2101 aaagatacat ttaaaccaac aacttctatg ttgtattttt ctattatatc atcaggtaag
     2161 tcacatgcac tgtccactat caattttatt ttatccatat taacctcact ataagatatt
     2221 attttattta ttcagtcggt cgtacgaccg atttaattat tattttatat attgaatgaa
     2281 aaaaagtcaa tagatataga ttctcgcttt attgacaata aatatccata agcttataat
     2341 gttattaatt tgttataaat atacatggaa aggtaggttt tatgaaaaca aataatatga
     2401 aacaggctat tttatcatct gcaaccaatt taatagccaa aaatggagtt caaaatacta
     2461 gccttgctga tattgcaaaa gatgtaaata taagtaaagg tactttgtat tatcactacg
     2521 catctaaaga tgatattata tatgatattg cggatactca tttagaagtt ataactagtg
     2581 ctatactaaa ctgtgtaaaa aatgtcaaat caaaaaattc tcaaatagaa atggttaact
     2641 taatcttaga gaaaatatct actatagaaa gtcgtggtag ggtacatatg tatcttattt
     2701 gtgaggcaat tacttcaaat aatgatttaa aggaaagaat aagattaaaa tatattgaat
     2761 ggagaactac tcttaaatct gaaattgttg aatcactaga aaaatataat agtagcgaaa
     2821 atgaacagga tgctgaatca ttttcttttt tactcatgtc aatagttgat ggtttagttg
     2881 tccaaagttt attaaaaaca gaaaaaatac cttatgaaaa tatagcttct tttttagtta
     2941 atcattggat ttaaacttaa tacttagata attaataaaa gtgaactatt taagttatct
     3001 actttaacag ttcactttct tatttattct caataatttt tacttaaaac attcaatttt
     3061 tatttaaacc ttcatggaat cacaaaataa aaatctctta taaatttaaa atgtttttat
     3121 ttatgttcat gtgtatgcac atctgaatgt tcatgaccat gtgtatgtgt atgctcatgg
     3181 ctatgctcat gagtacctga aaaaccatca tcatgattat ggtcgtgacc tacaccattt
     3241 acatgctcat gttcatgccc atgactgtga gaatgttgat gtgaatgttc atggttatct
     3301 cctccacagc atccttctcc tccagtccca caacacccac ttgtatgtgg attatgatga
     3361 tttcttacta caaatctctc tcttaacata attcttatac ctcctagtat attattgagt
     3421 acttcatgta ccccattaac tacagtatac atctaactag gtataatctc aatacatatg
     3481 gcatttttta acatatataa ttaacatata taatcttaaa gaaatttatc cttaaaacct
     3541 ccacctttta cttcttcaac gttattgaca gaaataaaag cttcactatc catatcataa
     3601 attaaatctt taaatttacc tatttcattt gcagatatta tgcaataaat catctttttc
     3661 ttattttgtg tatatgcacc ttctgcctcc aaaaaagtga ctcctctacc cattttattc
     3721 attatcactt tggatatttc atcatattta tttgatatta aaagtataga tttttttcta
     3781 tcaaagcaat cttttcctat atccatagta aaagaaatta tatacattgt aattaaagtg
     3841 tacatcacca atttagctcc aaataaaaaa gctcctgcac atactacaaa aaaattaata
     3901 aataaaaatg tatttttcat aggtatatca tactttcttt tcattatggc tgctattata
     3961 tcaagtcctc cagcagtagc tcttgcttta aaagttaacc ccatacctat accagttaat
     4021 gcaccaccat aaatacactg tagcatagta tcatctattg ggaaatattt agcaatatct
     4081 tgtgttagcc ccagtgctag agaaaatagt aacatattga caaaacttat tagcaaaaat
     4141 ttattatcaa aatactttct agctataata aatattggta tgttcatcaa gaaacttgat
     4201 attccctgat taataccaaa taatttgtat aatataacac aaattccagc tactcctcca
     4261 cttaataact tggctggtgc tagatataaa tttattccta aggttgatag aatcataccc
     4321 aatataataa gacaatattg taaaaatata ttattatcaa tacttagctt tttactttca
     4381 ccatacatca aaacgcaccc ctttgcataa taaataaacc accctgcata acacacgata
     4441 aatttacaaa taaatttatc agaagtagtt tcctaaaaat attattcatc ctcacttaaa
     4501 cgtttattca ttaatattct atcacatgac ataaataatt tatatgattt atatagattt
     4561 atttattgta aattttggaa tattaaaaca ttttattgaa tagataatta tattacataa
     4621 aaaatcccta tagaaatcta taaactaatt tctataggga ttttggttaa ctaactatta
     4681 tttttatttt tttataactt ttatacctgt catatggtca tttaagtttt gttcctctaa
     4741 agatttatca tctactctgt ttatttctaa agctaaagtt tcagacttga tatattcatt
     4801 aaaatggtca acagccttag caatttcatc atcaccacag tattctatta ctatattatc
     4861 taatacttca aagttattag attttcttaa ttgctgtact ttagatataa attctctagc
     4921 ataaccttca ttaatcaact cttcagttag cttagtatct agtataacaa aaagattgtt
     4981 ttcaacagat acattaaatc cttccttagc agttatacct attaatacta aatccttatt
     5041 aaattcaaaa gcttcaccat ctaaatcaac tgttaaagtc tcaccttttt caagtttatt
     5101 agcagtttca tgagcattta atttacttaa tgctccaacg aataaattca tcttagaacc
     5161 taataatggc cctgcttcct taaagtttgg ttttaaacta aagttcatat actcatctaa
     5221 atccttagca aatattacct ctttaacatt aagctcttct tttattaaat caactacatc
     5281 acttattgta tcttcaaact taccatctac taacactttt tgtaatggtt gacgaacttt
     5341 tattctttct acttctcttg aagctctacc taaagtaact aagtttttag ctaaatccat
     5401 tttttcttct aatttagtat ctactaactc ttcattacat ttaggatatt cagctaaatg
     5461 aacagatact tctcctgtta agtttctata catttcttct gacatatatg gtgcaaatgg
     5521 agctatagct ctacaaagac catgtaacaa ttcataagtt gtattgtaca ctgctttttt
     5581 atcttctgtt aattcagttg cccagaatct tcttcttgaa cgtcttatat accagttaga
     5641 taaatcatca tttataaaat cttgtatcat tcttaaagtt ttatttaact caaatatagc
     5701 taaattttct tctacatctt tcattaaatt attgaactta gataatatcc atctatcaag
     5761 ttctggtcta tctttatatt ctacaaaaaa ttcagttgga tttatatcat ctgtgtttgc
     5821 atataaagta aagaagttat atacattctt catagtacct ataaatttac tttggatttc
     5881 ttttaatcca tccatatcaa atttagttgg agtccatggt ggagaaacat atagtaaata
     5941 ccatcttaat gcatctgctc catattggtc aaataattcc attggattta ctgtatttcc
     6001 tcttgattta ctcattttct taccatcttt atcaagtaca aggtctggaa ctaaaacact
     6061 cttatatggt gcttttccta atacaaatgt tgatactgca agtaatgagt agaaccaacc
     6121 tctagtctgg tctatacctt ctgatatata gtcagctggg aacaattggt caaagttttc
     6181 cttattttca aatggatagt ggtgttgagc aaatggcata gccccactat caaaccaaca
     6241 gtctataacc tctgttactc ttgtcattgg ttttccacat ttttcacatt ttaagtgtat
     6301 atcatctaca tatggtctat gaagttcaac tgtttctgga tttacatctt ctatagcttt
     6361 ttcagctaat tcagctcttg aaccaactga atctgtatga ccacattcac atctccatat
     6421 aggaagtgga gttccccaat atctagttct tgatattgcc cagtcattta gattttctaa
     6481 ccagtttcca aatctcttct ctcctacaaa acttgggaac cattttacac cattattatt
     6541 ttctattaat ttatctttaa gtttagtcat ctctatatac cagcttggtt ttgcatagta
     6601 tactaatgga gtttgacatc tccagcagtg tggataatta tgaactactt tttctttgct
     6661 atataattta ttttcagaag ctagccattt tattatttca acatctagcc catcttccat
     6721 tacaaatcta ccttcccaag gagtagatgt aaatttacca gtttcatcta ctggttgaat
     6781 aactggtaaa tcatatcttt tacctgtgtt gtagtcatct tcaccaaatg ctggtgctgt
     6841 atgaactata ccagttccat cttcagtagt tacataatct cctactgtaa taaaaaatgc
     6901 ttttttgtca acttctacaa aaggcattaa ttgttcatat tctaaacctt ctaggtcttt
     6961 acctttcatt tcttctaaaa cttcataatc ttcacctagt actttatcag ctaaaggttt
     7021 agatacataa tatacctcat cacctttttt aacttttaaa taatctacat cagcatttac
     7081 tgttaatgaa acatttgatg gtaaagtcca tggagttgtt gtccatgcta agaagtattc
     7141 atcagcatct tttttcttaa atttagctat aactgtatta gtttttactt ctttatatcc
     7201 ttgtgcaacc tcatgactag caagaccagt tccacatctt gggcaataag gaagtatttt
     7261 atgcccttca tatatatatc cttctttgtt gaatttgttt agtatccacc atacagactc
     7321 tatatagtta ttatctaaag ttatatatgg attatctaaa tcaacttcat aagccattct
     7381 ctcagtcatt attctccatt gtttttcaaa tgagaaaact gactctctac atttttggtt
     7441 aaattcagct attccatatt cttctatttg ttgcttacta gtaagtccta actctttctc
     7501 tacttgtatt tcaactggta atccatgtgt atcccaacca gcttttctct ttacttgata
     7561 accagacatt gttttatatc tacaaactga atctttaagc gttctagata aaacatggtg
     7621 tactccagga tttccattag ctgttggagg tccttcatag aatacgaaac ttgggtcatc
     7681 tttccctttt tctaaagttt tttcaagtat atttatgtct ttccaataat caaaaacttg
     7741 tgcttctgct tgttttacag aactgtctac caatggctta aatttcgcca tgtaaatcac
     7801 cctttcctac taaatattat aattccaaaa ttttttattc tttcacataa atttaaatac
     7861 taaaaaactc gcccccttta aaccaaaggg acgagttata aatcgcgtta ccaccctaat
     7921 tctatatgta aactaaatac tagctacata taacactcta atagtataac ggtactaacc
     7981 ggcacagctt agtagaattt aatcgttcag cctgcagctc aagagtgatt tttatcttta
     8041 taaattcatt ggttctcagc atacccaatt ctctgtaaaa ttatctaaag ataaatgtct
     8101 ctatcaacgc ttttaaaaat ttatctgtgt ttatatgaaa ttctataaca aataatttga
     8161 atagtcaata tatttttgaa aaatataata atttctattt ttcttacatt tttatcctat
     8221 ttttaatttt tgttatagat ttctcaaact cacacaatta ttctacataa aaaataaaca
     8281 aattacaagc ttattctaaa gttgtagcag cttcatcatt actataatta tacactgttg
     8341 ttccttcaaa tgcgtttaca catttttcat ctacatcata aaaaatctca tcaacacttc
     8401 ttatttcatc ttctaacagt gatttaaatt tatttctaaa tatcttaaat tctttgacta
     8461 aagcatcata ctcttttctt atctctataa cacggttatt agcttgttct ataatttgtc
     8521 tagcttttaa atcagcttct tcaacaatta tcttagcctt tttattagcc gcgctacaag
     8581 tgtcttctgc tgcactttga gcagttatta aagtagcttt tagtgtttct tcaatatttt
     8641 catacttgtt tacttggtct tgatataacc ctaatttttc tttaagagct gtattttccc
     8701 tacatagaga ttcataatct tccttgatga tatctaaaaa ttcatcaacc tcttcatctc
     8761 tataccctct taaacctttt ttaaactctt tattttctat ctcaattgga gttagcatta
     8821 catttcctcc cagctataat ttaaattagt ccttatacaa ttaatcttgc ttgaactttt
     8881 atttttcctt ttttagtaat atctcctatt tgtgttacct tagctcttcc tttgcctcta
     8941 atagaaatca aatcatcgtt ttttactatt ttagaagttg aagttatctt ttcataattg
     9001 acatggactt tttctccatt tatgtatttt gcactatctt gtctagatat attgtaaata
     9061 ccacttatca cacaatctaa cctatctgaa gatactgtaa atgatacttc tttatatttt
     9121 gaagaactat taacaatatt ttctctggaa atttccttta caattacatt atttcttgaa
     9181 accttttcta agtttacaat tataaaatcg caaatatcac tacttacaac tacttgacaa
     9241 aagctatcat gtataattat atctccaatt ttttctcttt ttatacctag acccagtatt
     9301 gaccctaagt aatctttatg agatatattt ttaaatttga aattcccttc tatttgtaaa
     9361 aatctaagag tatcttctat atcttcatat tccatgtaaa atggataaat aaatacagtg
     9421 cttctttcag cttgttcata acctccatcc acactgtatt ttatttcatt tgtagaattt
     9481 aatattgcta cagcattttt cacttcatat ggattaagaa actccgtaga cttaacatca
     9541 tagtttttta tacaactatt agccttgtct atcactttaa acattttatt tttcaagtct
     9601 atatccttta tgtgatttgt tagttttaat ttatccatcc tatcacctta gaatataacc
     9661 aatactaatt gtcttaggaa catcaataat actattgcaa ttaaaggaga aaaatctata
     9721 ggtccatttg tatatttata cattatagac cttattggcc cttctattgg ctctgtaaga
     9781 ttttccaaaa tatcaaaaat tttactttgt cttccatttg gaaaccaagt catcaagctt
     9841 tttataacta tcatccatgc tattatatct aaaagataat aaagtgcaat tcttattgtt
     9901 cccatatatt aaccgccttt ttactattta ttttgccaag ggaagaatgc tttactttct
     9961 aattcttttt tcatatttgc atctatatcc acattattag gagctagtat aaatattgat
    10021 ttagaaacct tttgtatact tccatctaag acataaactg ccccattcat aaagttaaat
    10081 atagatttgc gaacttcaga attagtcaat ccttccaaat tcactataac tgcccttctt
    10141 tgttttagat ggtctgctat agttgcagct tcatcataaa ccttaggttc aactattaca
    10201 actttcattt ggctagttgt atgtaaattt acaattttat ttgcttttgc agtactaaag
    10261 ccactgttca tttcttcttc ttgaactata tcgtccatgc cactttcata ttcatcttca
    10321 atgtaatctt cttcttcatc aactatccaa ttcttaaatt tagatattat tccatttgac
    10381 attattaact tcctccttaa aatttattca aatttgagta attatttttt attgtagttt
    10441 ctttctccaa agattgatgt tccaactcta acaatggtag ccccttcctc tatagcaatt
    10501 tcataatcgt gactcattcc cattgacaaa acatccatcc ctacattagg gatgtttagt
    10561 tcttttattt gttttgataa atctttaaga cctttaaata cacctcttat ctcatcttca
    10621 tcttctataa aaggtgccat tgtcattaga ccctttattt ttatattttt atatttttct
    10681 gacacttctt tgacaaagtc tacaacttct tctctatcaa gtccatgctt agactcttct
    10741 ttagatatat tcacttgtac taagcaatct atttctctgt ttatttgttc tgcacgtttt
    10801 tgaatttctt cgcataatgc aattctatct aaagagtgta tcatacaaac tttatcgatt
    10861 atgtacttaa ctttattggt ttgcaaagtt cctatcaggt gccagtttaa tttatcacct
    10921 ataatatcat actttctagc taattcttgg ggtttatttt caccaacaga agtgattcct
    10981 tccctcattg cctcattaac taagtctaca tcaacagtct ttgtgactgc caaaagggta
    11041 atctctttgc tgtctctatc tacttttttt gcagctttat caattttgtt tcttatctca
    11101 gacaaattat ctttaataga acccattatt atcacctact ttatccttat atttgtgttt
    11161 atactgttag gtcttaaaat tattttgtca tatagattaa cacttgtaac tccatttatg
    11221 tcatttttat aataatccac aaatacagaa gattcattct cataagttat tccttctaat
    11281 atgacaaatt ttacactttg tgattgttca tcaattacat atacaccacg tttattgtct
    11341 tttgttttta tagaagtttt aggtattttt aaacattcca tctgattata tattatatca
    11401 aattcttcga cacttgtatc ctgatttgca acaatttggt tacttatttt taattttacc
    11461 attattttat tattattttc atatattttg taaacttttg catctaattt ttgctcatga
    11521 tattcaatta ttacattttg atttttttca aagttattag aatcttcttt actgatgtat
    11581 gtagatagat aattactctg gtcattggca tctttttcta tacttccttt tacatgaatc
    11641 tttgcatcaa ctttttcatt ttttaacacc aacgtatcta catttttgct aattatcatt
    11701 actaatatat taaatactaa atatataaga ataccaagta tcactaatct ggtatatttt
    11761 acctttttca aagactctcc ccctataaaa ttactttcat tataagtact tcttctaaaa
    11821 aatgtttatt ccttttttat aagctaatat tttacgttct aataattatt ttttccatat
    11881 taaatttatt ttttatcaat cttcctatac aagactgcat tatttatata taattgtatt
    11941 ataaagtata agtttaattt acattttata cgttaagctt actttatatg tttttatagt
    12001 tatttttatt tttagatgca atataaaacc aattaagatt taaaataact tttgttatgt
    12061 tgacatttat ttaggtaatc atttatgaat taggataacg ttatcataac aaaagtttat
    12121 gtcctaaaaa aaatacaagg aggattttct tatgtcagaa attttattag gaacaggttt
    12181 acttttattg gttctatgtt gtttcacaat ttttagttac aaggccccat atggtatgaa
    12241 ggctatggga gctctagcaa acgctgcatg tgctactttc ttagtagagg cattcaacct
    12301 ttctttactt ggtgatgttt taggtatgga attttttaga agtgttggag ctgctaatgg
    12361 tagtcttggt ggagttgctg ctgctatact tgtgccttta gccttaggtg tatctccagt
    12421 ttatgcagtt ttaataggat tatcttgttt agatttttct atattatctg gattcgttgc
    12481 tggatacttt gtttctttct taattaagtt tatagagaaa aaagtgccac ctggagtgga
    12541 tttagtggtt gtaattcttc tatgtgcacc gctatctcgt cttatagcat caaatgtaga
    12601 cccattagtt aatagtacac tactacaaat tggacaagtt ttgatagaag ctaccaacat
    12661 tagccctata ataatgggaa ttatactagg aggtctaata acagtagttg gaacgtctcc
    12721 acttagttca atggctctta catctataat tggtcttact ggtcttccta tggcaatagg
    12781 agcattaggt gcttttggtt cagcttttat gaactcaaca ctatttgcaa aattgaaatt
    12841 aggttctaaa aaagatatta tatctgttgc tattgaacca ttaacccaat cagacataat
    12901 ttcagctaat ccaataccta tatattctac taatttcttt ggaggagcct tttctggtat
    12961 cattgtagcc ttgatgggtc ttgtaaacat gacaccaggt acagctacac ctgtggctgg
    13021 ttttgctata atgtttgctt ataatccagt tcaacaagtt cttataacag ctggtttatg
    13081 tgctttattc aatgtaattg caggattttt aggttcaaaa atattcagta cctttaaagt
    13141 tcttactgct ggtgaaatac gtggtactga tgtagaagaa gaaattgctt aaaaaacaat
    13201 aagatttata aatttatatc tcaagtagta gagttttata ttgtaggcaa ttttattaat
    13261 tgcaaacgtt ttatttttta tttatgttta tgattattat gtgtgttgaa tatttagata
    13321 aatttttatt tattattttt atttagattt ttgtttagtt ttttatttgg ttgatatatt
    13381 aggaataaaa attagtatag tttattacta gttaagtagc ataacttttt agatacataa
    13441 aaaattttat attaattaga tattattaaa ataaaagcga tacatgaatt tttaaatgtc
//
LOCUS       DAELNY010000105        13259 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.105, whole genome shotgun sequence.
ACCESSION   DAELNY010000105 DAELNY010000000
VERSION     DAELNY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.105"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    227..458
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            566..2047
                     /locus_tag="KPE55_002435"
     CDS             566..2047
                     /locus_tag="KPE55_002435"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG0493493.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2057..2950
                     /locus_tag="KPE55_002436"
     CDS             2057..2950
                     /locus_tag="KPE55_002436"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG0493494.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3074..4135
                     /locus_tag="KPE55_002437"
     CDS             3074..4135
                     /locus_tag="KPE55_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG0493495.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4290..4802)
                     /locus_tag="KPE55_002438"
                     /pseudo
     CDS             complement(4290..4802)
                     /locus_tag="KPE55_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            5322..6257
                     /locus_tag="KPE55_002439"
     CDS             5322..6257
                     /locus_tag="KPE55_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG0493496.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6349..8220)
                     /locus_tag="KPE55_002440"
     CDS             complement(6349..8220)
                     /locus_tag="KPE55_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG0493497.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8410..9336
                     /locus_tag="KPE55_002441"
     CDS             8410..9336
                     /locus_tag="KPE55_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0493498.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9475..9621
                     /locus_tag="KPE55_002442"
     CDS             9475..9621
                     /locus_tag="KPE55_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493499.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9726..10250)
                     /locus_tag="KPE55_002443"
     CDS             complement(9726..10250)
                     /locus_tag="KPE55_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0493500.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10303..10527)
                     /locus_tag="KPE55_002444"
     CDS             complement(10303..10527)
                     /locus_tag="KPE55_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493501.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10724..11848
                     /locus_tag="KPE55_002445"
     CDS             10724..11848
                     /locus_tag="KPE55_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG0493502.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12322..13146)
                     /locus_tag="KPE55_002446"
     CDS             complement(12322..13146)
                     /locus_tag="KPE55_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493503.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         4668 a         1912 c         1648 g         5031 t
ORIGIN      
        1 ttacagatat ttaatagtta tcttttaatt actttcatta aaaacagctt catcactatg
       61 aaaatctagg tttttaaact tttaaaaaag ttaaaaaaat atttttctaa tactcaattt
      121 gattacaatt tttttacaat ttaaaaaata ttttttccaa atttttatat taatacttga
      181 ctttgtctgt tttataaact agaataatta ataatattaa gaatattgaa aggcattgat
      241 taggaagagt aaatctgaat cgattaccag agaaggaaac tactagctga aatgtttccc
      301 taattagaag gattgaaaac tacctaggag cttctatccg aagttatctt aatgatttta
      361 gtaggaatag acgtttttat tacttgcgtt aaagtatcaa gtggattttt taaaatcaat
      421 ttgggtggaa ccgcgggaaa cagattctcg tccctttttt agggatgagg gtcttttttt
      481 attacctaaa ttcagttttt aaaatattct gtatctaatt taattagcta attaaaaatt
      541 taaaatatat ggaggattat aaaaaatgta ttattcaagt acgagaggca ccgaagaaaa
      601 agtaactgca tcacaagcaa taataaaagg aatatcaaat gatggtggtc tttatgttcc
      661 aagtgaattt cccaatgtaa aaaatgaact tataaattta gtaaatttaa cgtattctca
      721 gatagcattt tttgtactta gtaaattttt atgtgatttt actgaagatg aaattaaaaa
      781 ttgtatagaa aatgcatatg atgaaaagtt tgattgtagt agtatagctc cattaaataa
      841 agtaaatgat acttatttct tagaactata tcacggtcca actttagcat ttaaagatat
      901 ggcacttact ataatgcctc atcttcttaa aacatctata aagaaagata atttagagaa
      961 agatgttgtg attcttactg ctacatctgg agatacaggt aaagctgcac ttgagggatt
     1021 taaagatata gataaaataa agataattgt attcttccca gaagatggtg taagcccagt
     1081 acaaaaactt caaatgaaaa ctcaaactgg aaaaaataca tatgtagttg gtataaaagg
     1141 aaattttgat gatgcacaat caggtgtaaa gaagattttt tctgataaag aatttaatca
     1201 aaaactattg gataataatt atatattatc ttctgcaaat tcaattaata taggaagact
     1261 tcttccacaa gttgcatact atttttattc atatatgaaa ttagttagca atagtgatat
     1321 aaatttaaat gataaaataa attttgttgt cccaactggt aattttggta atattctagc
     1381 tggatattat gccaaacaaa tgggtcttcc aataaacaaa ttaatatgtg catcaaatga
     1441 taataatgtt ctttatgatt tctttaaaac tggtgtatat gataaaaata gaacattgaa
     1501 attaactact tctccttcta tggatatatt aatttcaagt aatttagaac gattactgtt
     1561 tgaaatatca aatagaaata ctgatattgt aaataagctt ttatcagacc ttagtgataa
     1621 aggagtctac aaaataagtg atgatatgga aaaaaattta gcctcattct atggagaata
     1681 ttcaaatgaa gaagaagtta aaagcacgat aaacaatgtg tttaaaaatt ataattattt
     1741 gatagatact catactgctg ttgcatacaa ttgttatgaa aaatataaaa aagaaacttc
     1801 tgataacaca aaaactgtta ttgtaagtac tgcaagtcca ttcaagtttt ctgaagatgt
     1861 tctaaagtct atagattgtg attttaaaga ccttgatgac tttgctataa ttgatagatt
     1921 atcaagtatt tctaaaatag atattccaaa acctattaaa aatcttaaaa atgcagaaat
     1981 actacataaa gatatttatg aaaaagatga attaaaacta gctataaaaa aattcttaaa
     2041 ggtataggtg attattatgt tagagattat agttcctgca acaagtgcta atataggtcc
     2101 aggatttgat tgtcttggaa ttgcgttaaa tatatacaat aaattttatg ttgaagaaat
     2161 tgaaagtggt cttgaaatag aaggttgtga agatgcttac aaaaacgaaa acaacttagt
     2221 ttatacatct atgaagtact tttttgatag agttaaacca gaaaaaatac ctgctggtat
     2281 caaaataaaa atccaaagtg aagtacctat atgtagaggt cttggcagta gtgcctcttg
     2341 tatagtagct ggagttatag ctgcaaatgc tctatcaggt gctaatttag ataaaaatca
     2401 attactaaat atagcttcag aaatagaagg tcacccagat aatgttgctc ctgctatttt
     2461 aggaaatatg attgtatctg ttactgataa tgagaatata cattatgata ttataaaaat
     2521 tcctgaagaa cttaaatttt gtgcaatgat acctaatttt aagttatcaa cagaaaaagc
     2581 tagaggtgtt ttaccaaagg aaataccata ttctgatggg gtattcaatg ttagtagagt
     2641 tgctttacta atatctgcct tactaaacaa aaactttgac ctacttaaag tagcttgtca
     2701 agacaaattg catcaagatt atagaggaac tctaattgag aactacaatg atattgttga
     2761 aaaatctgaa caattaaata gtattggagt atttttaagt ggagctggac ctactataat
     2821 gtctttaata aaagaaaatg atgatagttt tgtggataat atgaaaaatt acctacaaaa
     2881 actaaaatct gattgggaaa taaaagagtt atgttgcgat tctaatggag ctgttttaaa
     2941 tataatataa ttatcgtctt ctatacaaaa tataactact cttttattat aatttgtagt
     3001 ataataatta ggactaggta ataatattaa gtattataat ctagttcttt aaacatgttt
     3061 acattgagga gggattatca taaaagagtt aataattaga gatttagttg caaaagttcc
     3121 tataattcaa ggtggaatgg gcgttggtat atctaggtca tcactagctg gtgcagttgc
     3181 taagcttggt ggtgttggtg taatttctgg agttcaaatt ggatatgatg aggaagattt
     3241 tgaaactaat actataaatg caaatttaag agctataaaa aaacatattt ctaaggccaa
     3301 agaaatctca aatggtggta ttattggtat taattttatg gtagctatga aagaatatga
     3361 aacatacgta aaggaagctg ttaaagctgg tgtagacctt ataatatctg gagctggatt
     3421 acctaataag ctaccatcat tagtaaaggg aagtaatgta aaaatagcac ccatagtttc
     3481 aactgcaaaa gctgctaatg taattttaaa aatgtgggat agaaaagaga aaactactgc
     3541 tgacttaata gttgttgaag gtccaaaagc tggtggtcac cttggatact ctaacgagga
     3601 acttgataac atagattcta tagactatga taaggaattt gttgaaattt taaaggttgc
     3661 aaatacttat ggagaaaaat ttggtagaaa cataccagta gttgctgctg gtggtataac
     3721 ttctagtagt gatgttaaaa aatatataga tatgggtgca agtggtgtac aagttggtac
     3781 aagatttgtt gctacttacg aatgtgatgc ccatgagaac tttaaaatgg catacataaa
     3841 tgctagtgaa gaagatgttc atatagttaa aagtcctgta ggtcttcctg gacgcgccat
     3901 aagaaacaaa tttatagaag aagttaaaat aaatcgtcca gaaataaaga agtgctataa
     3961 ttgtttgatt ccttgtaatc caaaagaaac tccttactgt atttctcaag ctctaatcaa
     4021 tgcagttaaa ggagatgttg aaaattcact tttattttgt ggaaatgatg cctataaaat
     4081 tgataaatta agtactgttg aagatgttat caatgaattg atttctgaat tataatttaa
     4141 agaaaactaa attaagattt tatcaagcta aggagtttaa atacgttatt tattaatctt
     4201 tataaacata tttatgtcct tagctttttt gtgtcaattt ccttttattg aaattttaat
     4261 ttaattagag atagtattaa cccttataac taatcttctt ttacaagtac attggttatt
     4321 tcagctatat ataaagtatg ataatctttt tgtggatacc atcttccatc tataccttca
     4381 gcaataaaac attgaggttc aagtttctga ctatacattt ttttgcatat aatagccatt
     4441 ttagattctt taaaataagg tgtatcattt aagtgttcta tattaaatcc aatctatgat
     4501 atcttatcct cttctctacc agataccttt ccacaatatg aaagctcttt acgaaaatct
     4561 tcatcaaaaa atgtaagaga aaatttatct gtattatcta taaattcctt agtataacgc
     4621 tgtggtctta ttactacaaa agcaacatct tttccccaca taactccaag ccctccccag
     4681 ctagcagtca ttgtattcac tttaccttca ttttctgctg ttataagtgt ccaatcttta
     4741 cctatcaatc caaatggatt atttttaatc tcttctgcca aaatttcttt aaaatcactc
     4801 atatctctaa taacctctca ttctcttata tttatattcc ttttattttc gtgataaatt
     4861 ataaatgaag tctagttttg aaaataaaat acttttttaa atatattatt gcaaataagt
     4921 aaatatatat atactctctt attttttaat atatttaata atttcactca gaatatatat
     4981 attctttact caaattaaat ataacttata tactttatgt gtaaattatg gtaaaaatat
     5041 tatttaatca aatcaaaact tgatttatat acctggaaaa ggtattatat taaagagtgc
     5101 ttaaaatacg catttcataa atagctatat ataaaataat aaataaggag agtgatgggc
     5161 aatggatact agtccgttgc ggagagaatt ttttacattt tcatcactta ataatatcta
     5221 aaatcaaaac taatacatat attcagttat ataaattagt taattaatag atactattaa
     5281 atgatgagat ttatataaaa ctgaataaag gatgtgttgc catgataaga tattacaaaa
     5341 ctatagattc aaaattagag aaacttagtt tttttgaaga tggctgttgg ataaatcttg
     5401 ttgaacctaa tcatagtgaa atcaacgaaa tttctaattt attaaatata gatgtagaaa
     5461 gcatagaatc tgcactagat gaagaagaac gctcaagaat agatgttgaa gacaatcata
     5521 ctcttatact tatagatata ccagtagacg aaagtgactc taattcttct cattatacta
     5581 caataccctt aggtattata ctaactgaag aagctattgt aacagtttgt gatgcacaaa
     5641 ctaagatttt aaatgacttt atagttggtc atattaaaga tttttttaca tttaaaaaga
     5701 ctcgcttttt gcttcaaatt cttcacaaaa atgcagctta ttacttgcat tacttaagaa
     5761 aaataaataa aatgacaata ataatagagc gagaaattta caaatcaatg aaaaacaaag
     5821 aattggttca attattggaa ttagaaaaat ctttggtata cttctctacc tcactaaaat
     5881 ctaatgaact tgttttaaac aaaatggtta gaactgctgg tataaagaag tatccagatg
     5941 atgaagacct attagaagat gttatagttg aaaatagaca ggctcttgat atggctaaaa
     6001 tatatggaga tatattgagt agaattatgg atgctttcag tgcaattata agtaataatc
     6061 agaataatgt tatgcaaatt ttaacagtag ttacactaat tttctcaata cctacaataa
     6121 tatcaggatt ctttggtatg aatgtcatca acatgccatt ttctaataac ccaaacggat
     6181 tttggataat acttcttatt acctctataa tatgtatagt gattacattt ttcatgtcaa
     6241 gaaataagct attataaaat attaataaaa aagccattag aattaattga tttacattta
     6301 tcctaatggc ttttatatat ttaaatctat ataatcttat aatacttttt aactatcatg
     6361 ttcttcatct ataatcattt cagtttcaag cccttctagc attgaatcta aactttcttt
     6421 tatatttagt atataaattg gtacatactc ttcttcatct attacctctt cttgcaactt
     6481 aaatggagga attaatactt catcaaagtc tatatttaaa tttggaaagc cttctactat
     6541 ctcttcacaa ttatcgtact cttcatttgc taaagaaatg tacatatcta ggctcttttt
     6601 ataataaaga cctgcaagca tatactcaca cttcttactt ccatatttaa taccttcttt
     6661 ataatatttc tcagcttctg cgtcatcatt taattttaaa taaatttgac ctaaattata
     6721 tattgattca acacaacctt gtgctctagc cttttcatac caaaatttag aatttacata
     6781 atctttatag aacatatcat aaattaatcc taccatatgt tgagcataca cattattttc
     6841 attggcaggt gtatcaaaca tcattttaga ctcttccaat ttaccttctc taaaataaat
     6901 tctacctaga tgtatacgag cattcatatg gtttttgcta cttgccattt caaaatactt
     6961 tctagcattt tttgtatttc ctaagctctc atatatacac cccaatctat aactagacct
     7021 cgtatctcca ttttcagatg ctttcttata ccaacttatt gcttcttcaa tattatttaa
     7081 atcttcatag atatttccta atttgatttg acaagataca ttatttggaa tcctcgaata
     7141 atacgaaatc gctttttcta tatcttgatt ctgataatat aaatccccta gattttcaag
     7201 ggaacttttg catccaacag ctatagcatc ctcataatat ttaatagcat tttcataatc
     7261 tttttcttca aaatatatac ctgccagatt attttttgct tctgttatat catcttcagc
     7321 agccatttta taataagttt ttgccttctc tatctcattt tctctattat ataaattagc
     7381 aagtctatac ttagaaagtg tatgattttt ttctattgct ttctcataat aataaattgc
     7441 atctcccagc tcttcttttc tatcatatac atatgcaagc ctatactcag acttcacatc
     7501 atctctatca gcactctttt tataatactt ctctgcatct tctaaattat catttatttc
     7561 cataatcata cccaagttaa agcaaccttt catatgacct gacttgtaca actgtttata
     7621 taaacttact gttgatttat tatctttctt ttttccattt aatattgcta aattatattt
     7681 tgcatcatca tctaaaagca cagaaccttt tttatagtat tcatatgctt catcatcttt
     7741 ctccaaaaaa gtatataata cccctaaatt aaaacaagct ttacctaaat tcttgtctgc
     7801 tgctgttttt aaccattttt cacaccttac aaaatctttc attttaaaat ggcatacacc
     7861 taaattattt tgtgcttgta aataacctaa atttgctatc ttttcatacc aatatatagc
     7921 atattcatag ccatctaact tatagtacat attacctaaa tcaaaagcag catcttcact
     7981 tccactttta tatgccattg aataccactt ttctgcttct ttaaattgtt ttttactttc
     8041 atatatattt cctaaagtat attgagattt tacgtaatta tccaatgcag cactcttata
     8101 caactctatt gcgctttcca tcttacccat cttttcaagg agggcaccct tagtatgtac
     8161 gactttcggg tcattagata tattttttat tttttttata gaaaattttg tatttttcat
     8221 tattcgctac ctacccattt tataaaatat attctctcaa acattattat acattataat
     8281 ctacaattaa tgctataata ttgaattttt accttttgtc actatattgt aacattaaat
     8341 tttaaatttt ataatcatta gattttatta tgtaattggt atataatgta tatatagggg
     8401 ggacatttta tgaatttcag caatattttt atccaagttg cagttttatt tataattatt
     8461 ttagttggat attttgtcag aaaattcaac ttattagatg accactgcac atctaagctt
     8521 tcaactttga caatgacagt ttttttacca tcaatgataa ttagctccat gcaaattaac
     8581 tttgatagta aaatgataca aaaaatacta ttgttattat ttacatcttt gattatgtat
     8641 atagtttcaa ttataattgc atttttattg aaatacattt taaaatgtaa tgataaaaaa
     8701 gatttgggaa tataccagta tatagttgtt ttttcaaatg ttgcttttat gggttatcct
     8761 gtaattgaag cagttttagg tcatgaagca atattctata ctgcaatatt caatttgcct
     8821 tttaacttat tttctttcac tttaggaatt tatcttttat ctaaaggcag tacaagtaga
     8881 ggtttttcaa taaaatcact tataagccct gctacaatag ctattgttat tggacttttt
     8941 ttatttgtaa caggacttag gttaccccaa tttataaacg agccacttga gatgttggga
     9001 agtataacta caccaatatc catgattatc attggaagct tacttgctaa ttcttcagct
     9061 ctcgattgtt ttgtcaacaa aaaattatat atagtcactt ttataagatt attggtactt
     9121 cctgtgattg tgtattttat tctaaaagga tggataaatg ataagatgat tttagctata
     9181 ccagtagtaa tttcttcaat gcctgctgct gcaaatactg ctataatggc taatcaatat
     9241 gactccaata taacactagc ttcacaatgt gtgtttttta caacactatt ttcagttata
     9301 tctatacctt ttataagtat tttactctta tcttgattta tttttttatg tattatataa
     9361 taacatattc aataaattac aaatataaac attttattgt agtatattga tggaattttt
     9421 tcctagatat ttgtcaataa tatctatatg tattatagaa taggaggctt aaatatggat
     9481 atggatttac tctttaaaat agtatttgct atagttgcta taggtgtttc tgtaaaaatc
     9541 attaaaactg ttgctagttt agcttttaaa atagcgttga tattgctaat aattttattt
     9601 gtatatagaa tgtttttgta aacatttaaa aataagtttt ttaacacaat agagaagtaa
     9661 aatagattat ctttaatgta aattttttat taaagacact ctattttact tctctattag
     9721 cttatttaaa ttaaattttt attatatctt cccataatat aaacatcttc aaattttcca
     9781 tctctgatta cagcatattt tttagttccc tctatttcaa aacctagctt tttatataaa
     9841 tttaatccct tttcattatc cacaattact tctaatccta ctctagttaa cataatccaa
     9901 ttatctgcta tatctaaaag ttgttccata agcttcttac ctactcctct tccctgataa
     9961 ttctctgcaa cacttattcc tatttctgcg ctatgtctca atcgtggaga agaatttaca
    10021 tgtaacccag caaaacctat tacatctttt tctccattat tattagtttc agctacaaac
    10081 atataatcat tttctcccaa agaagctaac atagcttcag ttttatcgac tctgtcactc
    10141 attaatgcca aagtatttct cataacactg tatgttcttc taatttcatt aaaagcttgt
    10201 gcatcttctc ttcttatagg tcttattgtt atatctatag gcttactcat attggattcc
    10261 ccctcaaaca taatctttat ttttaattaa aattttacat ctttatttta tactgttcaa
    10321 atctatgtat gcttctacct gaggtacata tatcatatta tcttttatat gtgtacttcc
    10381 aaattctttt aattctgtac cttctttatc cttagttatt ttatatctaa tggattccat
    10441 accttcaact acttcagcat gaaaaaacat accttcatat tgtttaaatc taaaattaaa
    10501 attgtcttta gtaagtttta ttatcatatt tttatctact ccttatataa ttgatatttt
    10561 tcttattatt agttgtatat atactgctta ttcatattaa tatattataa cttatcattt
    10621 atttagtata aagttataaa tagatatatt tatacttatt ttacaatctt tcttcaattg
    10681 atatataata tgtttatata aactaatgta tgtgaggtga aatatgagca aaaaaacaaa
    10741 atatttttct ttaattctct ttcctattgc tttgctttta aattttatag ccagcaaagt
    10801 tccaaatatt gttgaaaaat attactccca attcatagat aaaataattg tacaaatatt
    10861 gagtaaagtt tctggaattt tcccattttc tctctatgag attacaatgt acataatagt
    10921 tatatctatc ttcttatttt tatgttgtac tttatctaca atttttaata agaaaaaaaa
    10981 attaaacata tatttaaaaa attccatttt aaatatatta tccattgtat caatagtcta
    11041 ctttcttttt gtagtgcttt ggggaataaa ctataatagg attccactag aaaccacact
    11101 tataaataac tataatttaa aaaacaatac ctctatccaa tcaaaacaat atagtgttaa
    11161 agagcttact aaattatata aattccttgt aattaaagca aatgaaacta gaaaactcac
    11221 attacaagat aaaaatggta ttgtaaaatc taatacagat tataatggtg tcataaatag
    11281 agctcaacta ggatacgata atatcttgga tatattacct ggtgttagtg gtagttattc
    11341 taagcctaag tatattattt catcaaatct aatgtgctat acaggaataa ctggtatata
    11401 ctttcctttt acaggtgagg caaatgtaaa tatagcaata cctgacttat atattccttg
    11461 tacagttgaa catgaaatgg cacatcaaag gggatttgct agtgaagatg aggctaattt
    11521 tatagcgtat cttacttcta taaaacaccc caatattgat tttaactatt ctggatatat
    11581 tttagcttta aattatactg cttctgcttt aagtaaggtt gattataatg catatgttga
    11641 tatttctgct ggcatttcag attctgtccg tagagattta aaaaatgaaa gtgaattttg
    11701 gcaaaaatat gaaggtaaaa ttaatgaaat ttctaatgag tttaacaatt cttacttaaa
    11761 agctaatagc gttactgaag gtactcagag ttatggtaaa atggttgatt tattgctaac
    11821 ttattatgaa ttatatccat ataactaaac actacaattc aaaattttat tcatatacag
    11881 ctgatattaa atcagctgtt ttctatttaa ataaattatt taacttcaag caatcttata
    11941 taatttgata gtacttatac tatctgtata acatttcaat tctaactatt ttagttaaaa
    12001 catttctatt atctttttta ataaatgttt taactaaaat ttttaaaaca taaaacttcc
    12061 acattatttc actatctata taaaaatcta aaaattcaca tataaaatta cataactaaa
    12121 aaatcataaa tttatagaaa acatatattc aaaataaaaa agcatgtatc aaaacttgac
    12181 acatgcttat aatcttatgt atatatatca ttttgtcaca ataaaaattt acttgtaaca
    12241 ctgatatttt taaacaaaat agtccttatt taattaaatt aacattaatt aaattcgtac
    12301 tcattttgct atcattaaac attacttata gtaattttct aatacactat ttgaaacttc
    12361 cttcccttga aaacttttca accaattctc atatgctgag tttttccata cactaccatc
    12421 attaaatgtt acttgtttta cacaagatat aatgtacttt acaccatgct ctccagttga
    12481 ttgtttccat ttatcaaaca atagaaaggc atcattttga ttcttacctg gtgctattgc
    12541 attttgcttt gcatattctt tctccatttg tttccattct ttcaaccaat tttctaccca
    12601 tgccttgcct tgttgttttt caaacatact tattatatcc tgtggtggtg attgttgcat
    12661 ttttctataa gtatgtgaat atgattttgg agacacagga gatatgcctg taacaatacc
    12721 atagtctaca ccagctgggc taaatccaac acttcctact tcgccatctg ctgccacatt
    12781 ttgagcatcc caatatagct caagtggttt tccattttta tcatatgcta agcatgtaat
    12841 ttcataatcc ataattgcct tttcactgct atttgttaag cttataacat tgctaggcca
    12901 atgatatgga gtatcatcat actgtatttt gctattattt ataaataatg gaatattctc
    12961 attataaaca agacctttac tactttgaat atctgatacc ttctttgtga cagtacttac
    13021 cgtatttgct aagtatacat ttgttttttc tgaattggtc gtactaagag aaaatgttgt
    13081 tgtcgttcca gtaatcagca aagcaacaac taaaataggt aagctagata tttttttata
    13141 tctcacaatt cttcacctct ttttctatta acttataata aaacaattta ccaaaacatt
    13201 aaaatctatg ttaacataat ttaccaactg ttctagtaac tatatcctat catagttac
//
LOCUS       DAELNY010000106        13075 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.106, whole genome shotgun sequence.
ACCESSION   DAELNY010000106 DAELNY010000000
VERSION     DAELNY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.106"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(24..500)
                     /locus_tag="KPE55_002447"
     CDS             complement(24..500)
                     /locus_tag="KPE55_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG0493504.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(503..907)
                     /locus_tag="KPE55_002448"
     CDS             complement(503..907)
                     /locus_tag="KPE55_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG0493505.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(882..1511)
                     /locus_tag="KPE55_002449"
     CDS             complement(882..1511)
                     /locus_tag="KPE55_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493506.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      1877..1920
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2021..2989
                     /locus_tag="KPE55_002450"
     CDS             2021..2989
                     /locus_tag="KPE55_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG0493507.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            2991..3554
                     /locus_tag="KPE55_002451"
     CDS             2991..3554
                     /locus_tag="KPE55_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0493508.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            3562..4251
                     /locus_tag="KPE55_002452"
     CDS             3562..4251
                     /locus_tag="KPE55_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0493509.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            4378..5106
                     /locus_tag="KPE55_002453"
     CDS             4378..5106
                     /locus_tag="KPE55_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0493510.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            5120..5794
                     /locus_tag="KPE55_002454"
     CDS             5120..5794
                     /locus_tag="KPE55_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0493511.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            5820..6656
                     /locus_tag="KPE55_002455"
     CDS             5820..6656
                     /locus_tag="KPE55_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493512.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            6672..7343
                     /locus_tag="KPE55_002456"
     CDS             6672..7343
                     /locus_tag="KPE55_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493513.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            7333..8565
                     /locus_tag="KPE55_002457"
     CDS             7333..8565
                     /locus_tag="KPE55_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493514.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            8715..9032
                     /gene="trxA"
                     /locus_tag="KPE55_002458"
     CDS             8715..9032
                     /gene="trxA"
                     /locus_tag="KPE55_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0493515.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            9049..9927
                     /locus_tag="KPE55_002459"
     CDS             9049..9927
                     /locus_tag="KPE55_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0493516.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            10197..10565
                     /locus_tag="KPE55_002460"
     CDS             10197..10565
                     /locus_tag="KPE55_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0493517.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            10568..10939
                     /locus_tag="KPE55_002461"
     CDS             10568..10939
                     /locus_tag="KPE55_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG0493518.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(11084..11539)
                     /locus_tag="KPE55_002462"
     CDS             complement(11084..11539)
                     /locus_tag="KPE55_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493519.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(11578..12843)
                     /locus_tag="KPE55_002463"
     CDS             complement(11578..12843)
                     /locus_tag="KPE55_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG0493520.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSIAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         5140 a         1424 c         2077 g         4434 t
ORIGIN      
        1 tcatagaaat aattagcttt attttagcta ttaagattta ttaaaaaatt atctatatta
       61 tcctgtgata ttctaaaact gtcataatta ccttttgaaa cagccttcat aataagttta
      121 tctaaaaatt tcattttttc taatcgagct tcactaccaa aaattccaat agcatttgca
      181 ctttctaata aggatgatgg aaaatttgat tttaaatatt tatctgcttg ttcagaaaag
      241 ccacagcaaa ggaaaatgcc aacttgtttt ttgtttaata actctacatt atcattacat
      301 agcacctgta ttttttttga aactgaacca acatatatag aacttccaat aataatttta
      361 tcaaattttg ataactcaat tttattatta ttattattaa tatcaacaat agtgacatta
      421 tctgatagtt tattttttag tatatttgca cagtcttttg tacaaccata tttacttgaa
      481 tatataataa tagtattcat aattattctc cctttcttaa agcattttct acagcttttc
      541 gtatagtatt actgctataa gttgcagaag ttatagcatc cacatctaaa gactgttgtt
      601 taataatact tgttgtaatt ttttcagcag gtttacctaa aagatggtcg tgttttaaga
      661 tattaatatt ttggatttta ccattattaa cttcaacaga cacagtagct tttaccaagt
      721 tattatcagc acttccagta taaatgccat ttttaacatt attaatatct atatctttta
      781 ctaccaagtt ttctggcttt aagttgctta caattacaat aataatgcat ataaatacaa
      841 agaatagagc aatagatatt tttacatatt ttttcttttt attagtagcc ttgttgcgaa
      901 ttttcattgt aaagtcctcc cactaaaata tctaagaaat tttctataag ttgagaaaaa
      961 ttatgtaaag aatatagttt attttcaaca acacataaaa cgaatcctaa caatgcactt
     1021 ataagcatcc atgagctttt atctatattt tttttgaata ttccttgttc aattccaatg
     1081 gaaaggaatt tatcaagcat ttttatacca ttattgctag gcgtattgtt tgtattttca
     1141 gatgcaaata taatattcat tctactatgc atgatagatt ttgccatctc tggttcacta
     1201 gaaagagttt ttataaagct agtcataatt tctatgattt gtttatctat tggtagtgat
     1261 gagcaattat tcataatgtc aatggcatta ctttctactg tgttataaag tttattggtc
     1321 atatcgctga taatctctgc tttgtctttg tagtaaagat aaattgtagt tggagaatat
     1381 tctatttttg tagcaatttt tcttattgat aaattttcat atccatgttg gtttataatt
     1441 tcgatgctag catccataat ctttttcttc atttcttctt tttcattttt acgtctttct
     1501 ttgatactca tataatcacc tctttgctta acggtgttaa gttaatttac actgttattg
     1561 tataatttaa ttttaatact gtcaataaat tattaaataa tataagttaa tatattttta
     1621 tggataataa attaatgtta ttaattgtaa tatttaagat attattgtat aataataatc
     1681 ggtttaaatg cgccacatct tattaaagtt ggttcataat tctaaataaa ctttaataag
     1741 atacattaaa atataataaa ttcttttatg ctataaaatt tattttatag ctattaaatc
     1801 agatgtttaa aaatttttta acggtctata gtttttaaga gtcttatagt aagtagccta
     1861 aaaatatgat tttacatctg tacttgttcg agaactccaa gtataaaaaa ctaaggaatt
     1921 atatagtggc agtatataat taataccctt agacacacct ttttgaaagt ggaaaaaaga
     1981 tgtgtagttc tcctcgacca caaatcaagg aggataaaaa atggaaaaga gaaaagtaat
     2041 aattgattgt gacccaggaa ttgatgattc tttggcaatt cttctggctt taaactcacc
     2101 agagctagaa gtaattggaa ttaccacatg ttgtggaaat gtcccagcaa atataggtgc
     2161 agaaaatgca ctaaaaacac ttcaaatgtg ttcttcacta aatattccag tatatatagg
     2221 agaagaagca ccactaaaaa gaaaacttgt aacagctcaa gatacacatg gagaagatgg
     2281 tattggagaa aacttttatc aaaaggttgt aggagctaaa gcaaaaaatg gagcagtgga
     2341 ttttataata aatactttat ataatcatga aaaagtatca ataatagcac ttgcaccact
     2401 tacaaatata gctaaagcac ttattaaaga taagaaagca tttgaaaatc tcgatgagtt
     2461 tgtatctatg ggaggagcat ttaggattca tggaaattgc tctccagtag cagagtttaa
     2521 ttattgggta gacccacatg gagcagatta tgtttacaag aatttatcta aaaaaatcca
     2581 catggtaggt ttagatgtaa ctagaaaaat tgtacttact cctaatatta ttgagtttat
     2641 aaatagactt gataagaaga tggcaaagta tataactgaa ataactagat tttatattga
     2701 tttccattgg gaacaggagg gaataattgg ctgtgtgata aatgaccctc tagcagtagc
     2761 atactttata gatagaagta tatgtaaagg atttgaatca tatgtagaag ttgtagaaga
     2821 tggaatagct atgggtcagt ctatagtgga ttctttcaat ttctataaaa aaaatcctaa
     2881 tgcaattgtt ctaaatgaag ttgatgagaa gaaatttatg tacatgtttt taaagaggct
     2941 ttttaaaggt tatgaagaca ttatagactc tgtggaagga gtgatatagt gtgaaaagaa
     3001 aaatatgtgt aaaaactatt acacttatag cttttggtat ttctataaat ataataggcg
     3061 cttttatagc tatgggatta agattacctg tatacttaga ttcaatagga actataatga
     3121 tagcttcact tttaggacct aaatacgctg ttgttacagg tgtgtttgga agtttaataa
     3181 gtggaattac gtttgatgtg tattctctct attttgcacc agttcaaata tcaaccggat
     3241 tacttgctgg aatagcattt aaaaagaatt ttttaagggg tttaaaaact ccactaggag
     3301 tacttttatt tgccattcca acatctataa taagttcaat aatttcagca tttttatttg
     3361 gaggtatgac ctcttcaggt tcatcatata tagtgcaaat cttgaaagtg ttaggactgg
     3421 gggatgtttt tagtgtgttt gtaacccaag tatttactga ttatggagat aagctgttag
     3481 cagttgtttt agtgaattta ggattaaatg cagttccaaa aactttaaag gtatcattga
     3541 ctggaggaaa atagatagaa aatggataga tatagtgaga taacaaataa aaatcaaaga
     3601 gaaattgttc tgcttaaagg atttccttgt atatggggaa agtgttcttt ttgtgattat
     3661 atagatgata attctaattt agaagaagag atgaataaat taaatctaaa ggttttaaaa
     3721 aacgtaactg ggaaatatgg agttcttgaa gtcataaatt ctggaagctg ttttgaatta
     3781 cctaaggata ctttagaaaa aataaagtgt attataaaag agaagaatat aaaaaaatta
     3841 tttttagaaa gtcattggtc ttacaaaaat aggcttaagg aaatgagaga gtattttgaa
     3901 attcctgtgg tatttaaaat tggagttgaa acctttgaca atgattttag aaataatatt
     3961 ttaaataaaa atgcaaattt taagactcca caagatgtaa aagaatattt tgattcacct
     4021 tgtattatgg ttggaataaa aggacaaaca aaagagatga tagataaaga tatagagatt
     4081 atcctaaata cttttgaaaa agcgacagtg aatgttttta taaataattc ttctagtata
     4141 aaaagagatg aagagttagt gaaatggttt gttagtaagt ataaattctt ggatgaaaat
     4201 ccaaatatag aagttttata caataataca gactttggtg taggagatta gcattaattt
     4261 agtttaaatt taaaattgaa aagtttctta tgtttttgaa aaagcttcta atagttagaa
     4321 gctttttttg tagagtttta tgttacaata agaaaaaagg aattaaggag caattaaatg
     4381 atgtttgata ggggaaaatg gaataaattg gattataatg aacttttaga atatatgatg
     4441 tctatacaag atataaaata tagagatttt aataaaaagc ttatacctgg aacagaaaat
     4501 ataataggta ttagagttcc taatttaaga aagttatcaa aagaaataag ccaagggaat
     4561 tggaaggaat ttttagaagt agcacaggat acatactatg aagaagttag gctacaaggt
     4621 atggtaattg gcaatataaa tagtaatttt gaagaaactt tatactatgt aaaaagattt
     4681 atacctaaaa tcgataattg gtcagtttgc gatggatttt gttctgattt aaagtctatg
     4741 aaaaaatata aagaaaatat gtatgatata cttaaaaaat atgtttattc aaagaatcca
     4801 tgggaaatta gatttgcatt agttatgttt ttaatatatt atgttgatga taaacatatt
     4861 aaagaaatat ttgaatactg taataatata caaagtgaag aatattatgt taaaatgggt
     4921 atggcatggt tgctatcaat ttgttttatt aaatgtgaag aggaaacttt tctctatata
     4981 aaaaataata atttagatga tttcacttat aataaaatgc tacaaaaaat aattgaatca
     5041 aatagagtag atttagaaaa gaagaatata ataagaagta tgaaaagaaa gacaagaaga
     5101 tgttgacgga ggaatattaa tgaataacaa tgaagacaga attgacaaat ttttagaact
     5161 tcaaagcaaa ggatttaaat ttgcagagat agctcaaaat ctaaagatta gtcaaagtac
     5221 tttaagaact tttttaaata aaaaaggata taagtcagaa aaaggtaaat acatattaaa
     5281 agacgaatta aattataata aaagtttgga taatagaaac ttggaagaag agaaagctaa
     5341 ggagtatgaa aaaagtaaaa agaatactat aagtaataga aaaaaagtaa gcaccaaaaa
     5401 aaagatttct aatgaaaagg ctttaagtaa aagtgttgaa caaattgagt ttaatgatac
     5461 aggtttaaaa aatgcaaagc caaaacaaaa tattaagcct aagaaagata gaaaaataaa
     5521 tataactcaa gaagatttag ataagctttg tgaagtatat gattggtatt tagaagtaaa
     5581 agattacaat ataaagccta aaaaagtatc taataaaaaa gatgtacata tagatgatga
     5641 acacatagat gaattaaaaa caacaagaat acgtatagat aaagaaatat ggaatgattt
     5701 tgaaagatta tgtagtaatt cgaattttga aaaacatcaa atacttacac aggcattgaa
     5761 agaatttatg aaaaagtata aaaatctact ataaaatact actatatgag gtgcttaata
     5821 tgaataaaat agtaaaaaaa tcaatttctc taatgattat attaacaata tttattttta
     5881 tgttgacagc atgtgaaaaa gatgaacagc ctgattcagt tgatattcaa acagaagata
     5941 aaaatgagat aaaaatagat gagggaaatg caaaagtatt gaatatagga aaatcagaaa
     6001 ttataaatat agatgaaggt gataaagtag atacatcaac aaaggtagaa aataattcaa
     6061 catttaatat atccaatgtt gaattgatat ataatgaata tgatgagaat aaaaaaatta
     6121 tatcttcgga tagtaaggct ttgttggaca tgactttaat gcctgggaaa gttgcttaca
     6181 tagaatgtgg gcataaaact tttgtaaagg gtgtagaagt ttatgcatat gaatatgagg
     6241 cagagggtaa gattgtgtat gttaatttaa aagaaaatac tattaatata agaaataata
     6301 atattaagct agaaaatagt agtcagtatg aggtgctatc tacatcagaa cttaaaaaag
     6361 taaatgaatc aaatgaggga attacgtatc aagtaaaagt aaaaaattca tcatcaaaag
     6421 atttaggaaa tattattctt aagacagcag aagttaatga aaatggagaa tatttaacag
     6481 taaatcgtgt accatcatat aagattttaa agccttcaga agagacagat atagatatat
     6541 tgtgttcaac aaaggcaaaa agtgttgaaa tagtgggata tacgtatgat gatataaaag
     6601 aaaaagccaa tgtggatatt gacttaaaat ctcataaagt taagatagac aaataaaggt
     6661 aggggatact tatgaaggtt ttaatagttg aagataataa gatactttta gaaagtgtag
     6721 tagaagaact tagcaagcat tttgaaactg aaaagtgtga agatggagaa gaagctttat
     6781 acttaataaa tcaaaatata tatgatttag tcattttaga cttaatgctt cctaatataa
     6841 atggatttga tatcctaaag aaaatgagaa ttaataatat agatacacct gtacttatat
     6901 tgacagcaaa agaaacatta gatgacaaag tagaagcatt tactatagga gctaatgatt
     6961 atttgacaaa acctttttat atggaagagt tagtagctag ggtatacgct atattaagga
     7021 ctaatggaaa gataaaagaa agaaacggat tagaatttaa atctttatat ttagatacac
     7081 tagaaaaaag agtgtatatt gaaaaagaag aaataaaact tcaaaacaaa cagtttaact
     7141 tgcttgagta ttttgtatta aataaaggtt caattttatt aaaagagcag atttatgata
     7201 gaatatgggg aattgattca gatgcaacta ttgaaatagt ggaagtatat gtaagtaatc
     7261 taagaaaaaa gctaagtaaa tatggatatg ataaatatat taaaactaaa cgtaaggtag
     7321 gatatatatt cgatgataaa taagaatgta tttaccagta ctaaaaatca tttaataaaa
     7381 atgtatatta ttgtagtagg tagtttttta ataatctttt ctatatttat atactcatat
     7441 tttaggggac ttacatatag tggaattgat agtgagataa atgatgaact tgaatatata
     7501 gtctctcaat ttaaaagaac ttcatttttg aacccaataa gactcaaaga tcctaaagat
     7561 atggtgtatg tatatgaaga tggaaggata agttactata ctcaaaatga atattttgat
     7621 gagttacttc cagatagaag actagataaa aaaaattcgt tttttaaata cacagaaaat
     7681 ggatatactt ttagagaatt aaatgtagat gtaggtagat accaaataca gataattaga
     7741 aatatagatt ctgagatgaa ttctttaaga cagcttacat ctgttcttat aattggaata
     7801 ttaatttctg taataattac ttactttgtg gctgtatatc tcactagaaa agctttaatt
     7861 ccaatagaaa cagcatggaa aaatcaagct aagtttatac aagatgcttc tcatgaatta
     7921 agaacaccaa taacaatagt ttcatcaaaa ttagaaagta tgttaaaatc cccagaaagt
     7981 acagtaaatg atgaagttga aacaatagct actgcaatga aggaaactag aagacttaaa
     8041 aaaatgataa ctgatttatt gtctcttact aaagaggact ctattgttaa agttaattta
     8101 gaagaaatag atttagaaaa gttattggaa gaaatatcag aagactatat tgacattgct
     8161 gagtttcagg aaaaaagatt cgtgtttaat tctaaactga agaataaagt tataataaca
     8221 gataaaaata aattaagaca acttatatta attttcattg ataatgcctt taagtataca
     8281 aaattgggtg atgagatagc attagaattg aaagaagata tagaggatga ggtaacttta
     8341 cttataagtg atactggtat aggcataaaa aaagaagaga tacctcttat atttgataga
     8401 ttttttagaa gtgaaaatgt aagaaataaa gatttagaag gttctggaat aggactttca
     8461 atagctcgca tgattagttt aaacttatct attgatataa atgtaacttc tgatgtagat
     8521 attggaacta catttgaatt gtcaatacca aaaaagttaa aataataaaa atataaaaaa
     8581 ttgtttttag aaatgaagaa tgggtatact aattagtata aaatataaaa agaccttata
     8641 aaagtaaatt tatatttata aatttcatag tataattata tagggataat aaagtttaat
     8701 aggaggattt aaaaatggca aaagttataa atacatctga atttagaagt agtgttgaag
     8761 gtagtaaggg agtagttgta gttgatttct ttgcaacttg gtgtggacct tgtaacatgt
     8821 tgggacctgt atttgcagaa ttaggtgaag agatgaagga taaagctaga tttgtaaaag
     8881 ttgatataga tgagagttta gaaatagctc aacaattcaa tgtatctaca gtaccaacta
     8941 tgattatatt taaagatgga aaaccagtag aaactttaat tggattcatg ccaaagaaca
     9001 aaattgaaat gcaagtaaaa tcatatttat aaaaaatggg tgagtattat gagatatgat
     9061 atagctataa tcggcagtgg tccagctgga ttgtctgctg ctataaatgc aaaaataaga
     9121 aataaaacaa ttatcatgtt tggtaatgat aatttaagta ataagcttgt taaagctcct
     9181 tcaatagata attatttagg tttttatgat ataagtggtg atgaattaaa agacaagttt
     9241 aaatcacata tagatagtat ggatatttca attgaaaata aaagaataaa taatatttat
     9301 gctatgggag aatactttac aataatgtct ggaaatgata tgtatgaagc tactactgta
     9361 atccttgcaa ctggggtaga gtatactaga ccaatcaagg gtgaagaaga atttctagga
     9421 agaggtgtgg gctactgtgc tacatgtgat gcaccacttt atagaaataa aaaggtagca
     9481 gttataggat ataatgaaga atctaaagaa gaagcaaatt ttttaagtga actcacttca
     9541 aagacttatt ttatacctat gtacaaaaaa gataatctta tgagaagttc agataaccta
     9601 gatgattcta ttgaagttat acatgacaga ccagtacaga tagatggaga taaacttgta
     9661 aacaaagtat cttttaaaga aaaccacatt gaagttgatg gagtattcgt tataaaagat
     9721 agtacggctc caagtgcatt ggttcctgga atagagattg atggcattca tattaaagtt
     9781 gacaataata tgaagactag tatagatgga tgctttgctg ctggagattg tgttggaaaa
     9841 ccctattctt atataaaagc agctggtcaa gggcaaatag cagctttaaa tgctgtttac
     9901 tatttagata aattaaagag ggcttaatat ttatattgat taacttttta aagatattta
     9961 taaaaaactg tcttgaaata attttattat ttcaagacag ttttttattt ttaagttatt
    10021 aataaaagta ttttaaaatc tatatgtaat tacttttatt aataaaactc aaagttatat
    10081 tagtaaaaat tggaattatt tataatatac tccaaaattt aaaaataatg gttgaaaaat
    10141 ctaattaaga gtactataat ttaattaagt aattacttaa tcaaaatgag gtacttatgg
    10201 aaaacttggt taaaatattt aaagcattga gtgatgaaac tagacttaac atacttatat
    10261 tagtatccaa gagaaatata tgtcaaaagg ggatttctaa atatctaggg atttcagatt
    10321 ctgctgtttc acaacatata aaaatattaa aagatgttgg gataataaca ggatataaag
    10381 aaggatacta tgttttatat catattaata aagaatcatt taatatatgt gtagatttta
    10441 taaattctat gttaagtaat tctagtgaaa gctttataga tgtatttgat gtaaatacaa
    10501 tacatctagg atgcagtaaa gaatgtaaat ctataaaaaa atgttgtaag agaagggaga
    10561 aataataatg aaagtatgta taccagtaga ggagaataaa ggattggata gtaaaccata
    10621 tggacatttt ggttctgctc caatttttgt tgtgtgtgat ttagaaagtg gagaagttaa
    10681 atctttagat aatggagatt tagatcatga acatggaaaa tgtcaaccat taaaagcatt
    10741 gtcagggaca gctgtagatg cagtagttgt tggaggaata ggacaaggag ctataattaa
    10801 attaaatgga atgggaataa aagtttacag agcagaaggt gatactataa gtgctaattt
    10861 agacttactt aaaaatggaa aattagtaga atttccatca gaccatactt gctcacatga
    10921 cggatgtgga catcattaaa aaaaaacagc tgatgaaata tatacttcat cagctatttt
    10981 ttcttttaat aaatacgatt ttattctttt aaaaccaaaa tttagatttt tatgtttttt
    11041 gtagtatcct taataatatt tcaagtttac aagtttacta cttttagatt ttttttgtat
    11101 gtttttctat atagtcaata tcatttaata tgtcattgag ttctttatta agtgttttta
    11161 aatcatcacc atctacattg tagtttaaaa atagttcctc tacattatca atgatagaat
    11221 tgacttttct agtaacttca acaaatgtct taaagttact actattttta gcatttagaa
    11281 atttttttaa atcctttgat ataaatgata ctatttcata ttcatcatta aatagacctc
    11341 taccaatagg atatggttca attctctgta aaaaatattg gtctttagca ttaatattat
    11401 aagtataaga tatttcccaa ccgtctccta aatcaaactt gataaaatat aatgtactac
    11461 ttacttttga tattcttgtg gcaccaagct gtcttaaaag aacttcaatc gaatcacctt
    11521 gaacagtatt aatattcata tatacacatc ccctttagta ttattatact atatttttta
    11581 aaattttata attaaataaa cagtagatat aataatagat ataatcataa gtggaaaacc
    11641 tattttgaaa tactccttaa aagagatagg atgaccgtgt ttattaccta tacctgctag
    11701 tacaacatta gcagatgctc ctattagtgt tccatttcct ccaagacaag ctccaagaga
    11761 tgttgcccac catagtggca ttacatcaat accttcagct tgcatagtca aaataagagg
    11821 aattagtgtt gcaacaaatg gaatattatc taagaaagaa gagactatag ctgacaacca
    11881 taatatcaac aacattgtaa atacaagatg tccttctgta agtccaatca atgcttgagc
    11941 caatttgttt attatcccaa cttctactaa tccaccaact actacaaata atcccataaa
    12001 gaataaaatt gtagaccatt caatacctgc cataatttca tctacatctt gttttcctat
    12061 taaaagcatt atagatgctc ctgtcagtgc tacaacagaa gaatctatgt ggattgtact
    12121 gtgaaacata aaaccaaaaa gtatgaatgc tattaatata aggcttttta taagtagagg
    12181 tttatcttta acagattttt tttcatctag ttttaatatt gcatttttag ctctttcatt
    12241 cacaacaagt tcttttccat aaataaatct aaagcatatt atagttatag caagtattac
    12301 tataacagca ggacctaaat ttattacaaa atccataaaa ctaagattgg cagcactacc
    12361 aatcataata tttggagggt ctcctataag agtagctgtt cctcctatat ttgaagctaa
    12421 aatctgtgtt attaaaaatg gaactggatt aagtcctaat atttgtgtaa taactatagt
    12481 cattggtcca ataagtaaga cggttgtaac attatctaat acagctgata aaatagctgt
    12541 tataatagca aaacaaatca ttatcttcca tgggtcacct ttggctttct ttgcagtcca
    12601 gatagctata tattcaaata accccgagtt tttgacaaca gctacaaata gcatcatacc
    12661 aactaataca ccaatagtat tgaaatctat atggcttaat ccttcatcaa gtgtaagtat
    12721 attgaatatt atcattaata gtgcaccacc aacagctgct atggatctgt ttaccttttc
    12781 tgtaataata gctgccataa ctattaaaaa tatacatata gctattattt gttgaaattc
    12841 cataatataa gtacctccat ttgtattatt ttgtgcctaa tgctagtact atatatagta
    12901 ttactttttt attgtttttt caatggatgt atgaaagtat attatttaac aaagtttttg
    12961 gaaaattgac agaatttaaa gaaaattttt gttatttaaa taaattttaa aattattcca
    13021 ttgataataa tattaaacga caaacgaaaa aatataccct ttaaactaga attta
//
LOCUS       DAELNY010000107        12974 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.107, whole genome shotgun sequence.
ACCESSION   DAELNY010000107 DAELNY010000000
VERSION     DAELNY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.107"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..1324
                     /locus_tag="KPE55_002464"
     CDS             152..1324
                     /locus_tag="KPE55_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0493521.1"
                     /translation="MIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1660..2229)
                     /locus_tag="KPE55_002465"
     CDS             complement(1660..2229)
                     /locus_tag="KPE55_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0493522.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2632..3204
                     /locus_tag="KPE55_002466"
     CDS             2632..3204
                     /locus_tag="KPE55_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBG0493523.1"
                     /translation="MEYIINPCSGIKIKITRGQKIKIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINFFMYTKVNTDGSIEVCEPLSKASDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(3883..4785)
                     /locus_tag="KPE55_002467"
     CDS             complement(3883..4785)
                     /locus_tag="KPE55_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0493524.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(4912..5031)
                     /locus_tag="KPE55_002468"
                     /pseudo
     CDS             complement(4912..5031)
                     /locus_tag="KPE55_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5578..6240)
                     /locus_tag="KPE55_002469"
                     /pseudo
     CDS             complement(5578..6240)
                     /locus_tag="KPE55_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(6400..6936)
                     /locus_tag="KPE55_002470"
     CDS             complement(6400..6936)
                     /locus_tag="KPE55_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0493525.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7009..7422)
                     /locus_tag="KPE55_002471"
     CDS             complement(7009..7422)
                     /locus_tag="KPE55_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0493526.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            7915..8553
                     /locus_tag="KPE55_002472"
     CDS             7915..8553
                     /locus_tag="KPE55_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG0493527.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQGSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9161..9340)
                     /locus_tag="KPE55_002473"
     CDS             complement(9161..9340)
                     /locus_tag="KPE55_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0493528.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(9566..10099)
                     /locus_tag="KPE55_002474"
     CDS             complement(9566..10099)
                     /locus_tag="KPE55_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0493529.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            10416..10766
                     /locus_tag="KPE55_002475"
     CDS             10416..10766
                     /locus_tag="KPE55_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493530.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYEALVSVSFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            complement(11642..12214)
                     /locus_tag="KPE55_002476"
     CDS             complement(11642..12214)
                     /locus_tag="KPE55_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0493531.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(12276..12539)
                     /locus_tag="KPE55_002477"
     CDS             complement(12276..12539)
                     /locus_tag="KPE55_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0493532.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(12539..12760)
                     /locus_tag="KPE55_002478"
     CDS             complement(12539..12760)
                     /locus_tag="KPE55_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0493533.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         4550 a         2203 c         1555 g         4666 t
ORIGIN      
        1 tttaatctct acaagcataa attttattct aatattaatc aatattttca tagccttttt
       61 tctttatacg ttgacttcct attttataga ctttaggaat cattggattt tcaaaatcat
      121 attgattttg ggctactcta ttattagtca aatgataata tggctttccg accccgtaaa
      181 tattttgttt acactattcg gatattttat actacttata atatgtagca aaagcaaatt
      241 aattcctata atatcagttg tcatgatact ttatcctatt ataattgggc ttaattttct
      301 ttttataaac aaccctatat atgaggaact tatacactca aaagagtata ttttaatact
      361 ttcaatagtc cagataattt ctgctctaat taaattatta ttttggtttt taatacatat
      421 tttcctaaag aatagattgg tcaatataaa acaatactta tcaaataaaa tatggatttt
      481 tattgatatg gtatgtatat cttcattttt atcaatatta attgctatta ttctgccacc
      541 tactaattta atccctatgg gtagtaataa gaatattatc ttaggcaact taccttatgg
      601 cacttatcta atcatcttgt cagcaattct ggcaaatatc ggtgtgatac ttcttttaca
      661 gccattgatt gaaaatgtaa agatgaagat agaaaaacaa gcaaatcacc taaaagaaaa
      721 atactataac ttactagaaa atcaacaaat tcaaatacgc aaaatgcgac atgatatgaa
      781 taatcacttt caaatgattg aatcgtattt agaaattgaa gatattgctg gagctaaaaa
      841 gtattttaat cagttaaaaa taggtatagg tactcttagt ggcaaacaat tttgcaaaaa
      901 ccaagctctt aactcaattt taaatcttcg tcatacaagt ttggaagaaa atggtgttga
      961 tgtgcacttt aatatagata tagacaatca cttgggtata gaacctatag atttatgtac
     1021 tattttttca aattctttag ataacgctat agaagcatct ttgaaaattc ctgatttgtc
     1081 aaatagaaaa gtggtattaa aggcacgttg tgagaaggag tattttagct atttattgac
     1141 aaacaacaaa gtaaatccta tcaatagaaa acaagaacta tttatttcag ataaaaaaga
     1201 tagtaattct cacggatatg gtattgagaa tattagagaa attgttaata agtacaatgg
     1261 aaaattggat atttcttata ctgagagtga attttcttta tttctttata ttagattatt
     1321 ttagtctaaa attagattat cttatataat acaaattaga ttgtttgata ctatgtaaaa
     1381 ttataataac ttataattaa atatttacac atatattcat taacttaaaa tttaatcaaa
     1441 tgtgatattt tatttacctt agtaaaaatg tgtgtgtctc aaaataaact ttttagttga
     1501 tgaggcactc ttttttacat gaaatcaaat atatttttat cctttcaaca atgaaaaagg
     1561 gatttatctc tccattttga aacaagccct ttttctatta attactttct taaaaaatat
     1621 aatacgatat ataagataaa tatctaattg tacctatttc taattatatc tattagaata
     1681 caacaccttt gccttttcag taaactccct aacaaaatct agtttactat ctgtataagc
     1741 atcacgatta tgctcatact tcttccaaag gtccaactta agtttttcat actcctttgc
     1801 aacatctggg tggtcaatta agtaatctct aaaaaacaac tcatcattat cacctacata
     1861 ccttatatgc aaatgaaaaa ctcgttcttc aaacccatct actgtatacc ctttattaag
     1921 tgaaagccta ctactgctct cagacataca aatatatcca ttcgactcaa tcaaatcttt
     1981 aatatctttc atattataat caggaattat ctcaatcagt atgtcaacaa tgggcttggc
     2041 ataaatcgta ctcacagaag tacttccaat atgacttatt ctaacaattt tttgcattgg
     2101 caatattcca gttagtctat attgttcttc tttaaaccaa tttttccaac attctttgtg
     2161 agtagtcaaa aatataggaa acaagttcca taactcctct aaagtcatat cttccaactt
     2221 ctttttcatt agttacctcc ctttaaattt gtactttata ttttttcttt ttcaaattta
     2281 cttagttaat atttaacttt attttttatc aaatcatttt tctaattatg taaatttttc
     2341 taaatttagt ttttaatgta ttataacaga tttttaccct tcttacactt ttgtatctat
     2401 ttaaatatta gaacattttt tcttacttct aaatacagta agattgctta aataccaaat
     2461 aggagcaaaa aaactctgta atcttaaaaa aactacaaca tctttatgag aattaaaaaa
     2521 tgatagacta tgttatcaac attaagaaac agatagaaaa gaaacagagt atgtgctaaa
     2581 atgggaacat agaacagaat gacaaattgg aatttatgga ggacattaaa tatggagtat
     2641 ataatcaatc cttgtagtgg tataaaaatt aaaataacta gagggcagaa aattaaaatt
     2701 gtggatattg aaggaggaca ggtcgttgat ttttttgcag aaatgaaaga tgattcaact
     2761 gaatttatat ctccaggtgt tacaattgat tgtaatgagt cattaagatt acaagttggg
     2821 aatttaatat attctaataa gtatagacct atgtttaaaa ttttatatga tgatgtaagg
     2881 gaacatgact tgctacatcc atgttgtaga cctgagatgt acgatttctt ttatcacaat
     2941 ggagaaaatc atccaaactg ttttgataat attaatcgca cacttggaac ttcccatcaa
     3001 attattcacc ccattaattt ttttatgtac acaaaagtca atacagatgg gagtattgaa
     3061 gtatgtgaac ctttatcaaa agccagtgac aaaattattc ttgaagcatt ggaaaacatg
     3121 actttaggga ttgctgcatg cagtgtttca gaaagttctt gtaatagcag aaaatgtact
     3181 tctattaaga ttttggttga ttgacaaatt tcatttatcg aaaaaatatc aaaattaaaa
     3241 aatgacgtca aaggcgcaaa agggtaacca cttgcgctct caattcatta tggtttgtta
     3301 cgtatttatg ggtaaaaatc ccttgattct atgatgtttt tcagacacaa ttacaaatga
     3361 gtaaggattt tatactcata ctctaaaaaa tagcatttta ttgcgtaaca gaacgaaaaa
     3421 acagtgtgta cctttttctt gcaatacaca ctgtattcat agccactcta gctgttaact
     3481 gttaattatc tcataatacc cttactggta ttcatctaat cttaacccct tttaattatt
     3541 ttcatttata aataaattct attcttccca aaatatcgta attaaaatat attaattaaa
     3601 atatcccaat aagaatatct gaaaaatctt ctacctattt tttcaaacct tttattccca
     3661 ttcctcatac aatagtgcaa cttttagcac agtataaata taccattagt ccatataatc
     3721 tcattaacaa atatatacaa ttattttctt caaactcttt tatatctaag atatcacatt
     3781 ttcattttct tatattaatt ccttaactct ctttcaaatt accaattttt taatcaaatc
     3841 ttcattaaat attctatcat tattaaacat tcatcattat ccctaaaaaa tatgtgccgt
     3901 atgttcttta cacaaatttt ccatcttatc atacacagca ccactatctc tctcatctgt
     3961 catagtcaca attacatcac ttggtctaag aattgtctta cctctaggaa taatttcttc
     4021 tccacctctt tgaattgcta ccaaaagaca attttcaggc cactgtacat cctgaattgc
     4081 cttgttcgca acaggagagc caaacataac agagaactgg tctagtatct tttcgcccct
     4141 ctctttatgt acaggttctc cattcttttc aagtaacctc tcaagaagac tttcataaat
     4201 aggtttagac ttcataagtg tagccacaat atatgccaca attgaaatta ctgatagtga
     4261 aagcatttga ctaacagaac ctgtcatttc aaaaattaat atgataccag tcaaaggggc
     4321 acgcacaata gctgtaaaat atcctgccat agccaaaagc acaaagttat ttacataatc
     4381 gggattcatt ccaaaaactt taacacctac cataccaaat attccaccta taaatgctcc
     4441 taaaacaagc aatggaaaaa atattccccc tggtgcacct gacccaaaac taacagccga
     4501 aaatataaaa cgtataacaa aaatcaaaac aaccattcca aaaatatatt tatgacttgt
     4561 aagttcttca actaaattgt gtccactccc cagaacatca ggcataacaa gcccaagaat
     4621 accagccatt ataaatggaa tcaacagttt tccaaacgta ccaattttag gaatcttctt
     4681 gtataaagac tgcacatata gagtaaacca gttgtaaaat gcccccataa ctccaagaat
     4741 aacacctaaa atcactatta atccataata atcttgtggc aacacatttc caatctgaac
     4801 ctcccatgac taaagtcaca gggttcctgc ttcatagaat tttgcatact aaaatatgtc
     4861 ttactaattc tccacaggct atccccgtag ttcctacggt tcttacataa gttatttatt
     4921 ttgaattgtt attagtctta atccttcttt taatatattt atacttgcat ttatatctct
     4981 atcgtgagtt tcattacaac taggacaaat ccattctctt ataataatgt taacaaatgt
     5041 aattatttaa aacaattatt tagaaggttt tatatttaat tttaaataac atatttaaga
     5101 accgtaagtc ttaactttta tcatttttga ggttgtcgtt cacataagtt cgctactacc
     5161 tatgcagttc tcttatgaac ttcttacact ttcatgtaag cacagactat atcttatcct
     5221 tcggcattat ccgctaaggt ctacccactt ccactaccaa tagcttgtag tgtacttccc
     5281 tcaagaggaa tagtcgttga agtttctcct attcggagct tacctgctga ttgcccattt
     5341 ttaaagtact tagggtttaa ccttatacca tctaaccaat tttttctact ttcgtaactt
     5401 tcacacttag gtttatttca tccttatgtt ttagtttggt tagctttagg ggtttccagc
     5461 aattcaagta gtattggata ggttctatcc tatctctaca tataggtttc cctatatgct
     5521 gactatttta gctactaact catgactaaa gtcacaagtg ttcgtagcta ttttttaatc
     5581 aaactggaat acagactcca ttcctaggaa attggctgaa atatagtctg ctgtcaaaga
     5641 tgctgtcatc acagaaatta aaacagatac tgaaaagttt ttatggactt cttcaagtga
     5701 aaacataaca cccgctagtg gtgcatgaaa ggctgctgat aatccagcac ttgccccaca
     5761 ggttaacagg aatttttcct ctgtcttccc tcggtcaaga gctctagaaa tacctttacc
     5821 tgccatagca ccaagctgaa tggaaggacc ttcccttccc aaagcaagac cacccatgag
     5881 tgataagaat cctccaataa attttgttgg caacactcgc caccatactt gttttatttt
     5941 ccctgtcatt tccccctcaa gctgagggat accacttcca gaaatcattg gctcccagtc
     6001 caccaacttt gatacaatcc acgcaagtac tgcaagtact gcaaaccaag ctattatttt
     6061 tattgtagaa ccttctacaa attttaaaat ttggctaagc caagtaccag cctgccctaa
     6121 aagtatcctg tataaaagca caatcaaacc agccacagcc ccaaccataa gtccttcacc
     6181 aatcaaaatt acctgaaagt gctctgcccg tttgataata tgagatgtat cctttttcat
     6241 actgttcctc cttcatccaa taattctaca caatctgaat ctccaatgac taaagtcatg
     6301 agtgtgtaca gatatttttt aatcaacaaa aatagatttt tatttactaa atataagaaa
     6361 ataattgctc aatttctcaa taaatgtgaa tttattcttt taggtattta ttctccacag
     6421 tcgtgcttct tccagcaaaa tatttattag ttcttgcttt ctctgtgtat agattttacg
     6481 attgtcctca aactgtaatg ctaatttttg tttatgacta tcatagagtt ttgctttgtc
     6541 tgtaaaagta tttagataat ctcgaaaatt gatataatta ttccatgcaa cactattcca
     6601 ttcaacaaca tgaatatggt gagtacgagt atttttctca aaatctccca tagcaaaaag
     6661 taattgtttt ggtacatcct gacctcgaaa aacgattcca cattgctcta acctttcgag
     6721 gtataattta gcttcctcta aagaagcaac acctacaaca atatcaataa taggctttgc
     6781 gctaatactg ggaatagaag tgcttccaat gtgttgaata tcaatggcag tcttatccca
     6841 tatacttttc aataatataa tgacatcttt tgcactttta tcccatatca tctgatgttc
     6901 caataattta accataccac attttaaacc tatcactata taccacctca atataatcca
     6961 cctttatata tattacaatt ttttctatct tgataaacag tgatttagct atttcacttt
     7021 ttcttgacac tcgctacatt ccctatcatg aatagatata attcctccac atttagaaca
     7081 agtatatctc tccttctgct tttgcataaa cttctctaaa ccgtactgct gaacaaattt
     7141 actattttcc ataaggcttg cttgatatct cttgttataa cttttttcaa gattttttat
     7201 gagtttacaa ggaaaattga aacattcaaa acaataaaac aacccttttt gacagacaca
     7261 atcctttatt ttacacttac ggcagtgttc tggctttcct gtatcgccat tcaaacaacc
     7321 agcacatggc tttttgtgat aacaatgctt atagcaaacc atacaattca ttccacaagg
     7381 agcaaacata atcgtatcaa tactctcttt tggcattttc ataacatttt actcctttca
     7441 aaaatccaat ttgtcgaatt gttatatatt catattgtaa cataaaaaat agctaaaaaa
     7501 ttcatgcact ttcattgcca ataaattttc aaggaaatat gaaagtttaa aactaagaat
     7561 aaaaagagat aaaaaggaag ctactaatca aaatattcaa atacaagtaa tcaatataaa
     7621 taacttaagg tatatttaaa agtatatgat attaccagca gttttaaata caaatataac
     7681 cacacatgcc tcaaaaacac ttattacagt atcttagaaa cttagacaat acataagatt
     7741 cattaataaa aaagttctca atatagtcat taataaaaac gttctcaata tagatatatt
     7801 taaccatgtt ttgaatagca gataattata tgattaaaaa ttaaatatga catagcgttt
     7861 gtggaatata acacctaaat tatgttataa taataaaaca ggagtgtgat agtgatggat
     7921 gaaaaaatat tagagttatt acaggaaatg caaggtaata ttagtaatat gcaaggtagt
     7981 atcagtggta tcgaaggcac tattagcgat atgcaaagca atatcaatgg tatcgaaggc
     8041 actattagcg atatgcaaag caatatcaat ggtatcgaag gcactattag tagtatgcaa
     8101 ggcaatatca atggtatcga aaacactatt agtggcatgc aaggcactat tggcgatatg
     8161 caaggtagta tcagtggtat gaatggcact attagcgata tgcaaggcag tatcagtagt
     8221 atggatggca ctattaatag tatgcaaggc agtattactg atatgcaagt tgaaattaaa
     8281 gaagtcaata gtaggttaga caatatggaa cctgatatta aagacttaaa aactggacaa
     8341 gttgaaacta gtagtagact tgatactatt cagagtgatg ttacagaaat taagtcagat
     8401 gtaaaaatta acagaaatac attagataca gtgattaaac atacctcttc attagtaaaa
     8461 gatacatcag atattaaaaa tgatttaaat aatatagaga tagtcacatc taagaattcg
     8521 aatgatattg ctaaattaaa atcagttaaa taatatacta tagtaaaaaa tacctgaaaa
     8581 tttcacatac ttctttaaag ttataccaaa ttttatacag aaatactgat tatccgacaa
     8641 cacactttac ttataaataa tttattaaaa aaatactcat tttatcctaa aattaacacg
     8701 actaaatgag tcttgatata ataataaaaa ataagggaga ttggacacta aaaagttgtg
     8761 tatctccctt atattctata atatagttat ataatttttc agcctaaaat aaaagtccat
     8821 ctttgattaa aacacatccc cttatcactt ataaaaggat aaaatcttgt tttcattttt
     8881 ccatttatat cttcttgctt aagagtaata attttttctt ttgatacttt aagtgtttgg
     8941 ttaaatttaa ccaatcgact aagttttgtt caatatctat ttttcatatt ttccaatact
     9001 taatctattt tttataaata attctttcat ttctaactca cttacatttt ttacataaaa
     9061 cgaaacatat aaatttcaac cttatcttgt atggttctta atacttaatg agtagcttaa
     9121 gtatcaatca ttataaattt ttcccttaca tttaacactc ttaccatttc caaaatatac
     9181 aaaatatatt ttgaacattt gtacaatgat gaaatacaat acatgaaaat acaatatcat
     9241 aactcttgtc taaaaataga agttcttcaa cataaccttt cacaaaaaac atattatcta
     9301 agtatttttt gtctgctcct tgttttcctg tttgtagcat ataaattact gcatctaaac
     9361 atataactac tcacacgaaa aaagaaaata aacttccaaa atacaagctc taaaaactac
     9421 ctctaaagtt gtataatgtt tactaggaat aaggttgtat aacatatagt ttaaataatc
     9481 attttttaaa attgcaagtt attgctttca aaattagatg tttttatatc aaataaatga
     9541 ttaagaattt ctaaatttaa tggctttata atgcactccc tttctttggg ataaaataat
     9601 gttgcacaga tactttttct aaagataaca attttatttt tctatcaatt ccaccagcat
     9661 acccagtcaa actcccattt gttcctacaa cacgatgaca tggaacaata atagagattc
     9721 cattatgccc tactgcacca ccgattgctt gtgctgacat tttagatatt cctttttgtt
     9781 gagcaacttt atatgcaatt tctccataag tgattgtttt accatatggt atttgttgta
     9841 aaattttcca cactgtagtc tgaaaaggag tacctaccat atggagtgat ggtgtaaaat
     9901 tgggttcttt tcccgaaaaa taaatatcta accatttctt tgtctgctcg aaaattggca
     9961 attctttttc ttgatgttct tggtcaagat tataagcata atacttttga ttctcaaacc
    10021 aaagaccagt taacccaata ttatctgatg ctaaaagaat atttccaatt ggactatggt
    10081 aatagctaat atattgcata caattcctcc ttatattctg aaaacttcac tcaaatcatt
    10141 agacacttct tttctatcta ccattatgta aaaacatact aatataattt ccttctaact
    10201 acactttcgt tataatatct atcctcatat catattagtt ttatatttac ctgcatctgt
    10261 tcatcctttt ttccctttcc ctttgatgaa tataaaattc atacatttaa gcttcctact
    10321 gtattaactt ttatagaagt gtatattata attttatcac caaatagacc aaaaaaatat
    10381 cgatataaca tcaagtaata tcataatact ttcaaatggg aaaagtaaga ataggctgta
    10441 aaaaagaaaa gtatttttat gttagaaatc cagtaaaatc gttagtagat agaataacag
    10501 aaaacgaaca atttgctatt tatcatttta cacataaata taattggcgc aataaagtgt
    10561 tttctattct acaacgatta ataaggttag aaaaaagtaa aaatgaactg tatatgtatg
    10621 aagcattagt ttctgtttct ttcctttggc ttattatgat aaaaaatatt gttttgccac
    10681 caataaaaca caaaaatata attaatatcc atatgcaaaa aattttacgt tatattgaaa
    10741 aatactattg ttttacatta tattgaaggg tactattgtg aaaacttaat tttggagtac
    10801 tatcaagcag tggtaatatc aataaatcag agtgctccag atgatttgaa ctaagtttaa
    10861 atactatacc ttataaatat tttactgagt tttgtttatt aaaagcaact caatttctaa
    10921 aaggcacaaa tgagctaata agtgacattg ctataaaagt tgtttttcat caaataatct
    10981 attttagtaa atgttttaaa gaaaaaacgg gatattctcc aaaagtgtac agaaatataa
    11041 aaaaagagat atgatttttt atttcttttg attatccaaa gagtaaataa aaaatattgt
    11101 aaaaaactta ctaacaacgg caatgctttg aactatggta atactttgaa cttttgattg
    11161 gacttttttt gctaaaataa tatatttaaa tagcaaagta atctgtttaa tcaataaaaa
    11221 ataagagata aaagaaatca actaaaactt ttacctctta ttttgatagt ttatcagagc
    11281 taatatttac actcttttta tttttgatac ttcattaaaa atgatgctta ttctcttttc
    11341 atttttaata cttcattaga acttatgctt attctctttt cacttttaat tcttcattag
    11401 aacttatgct tactctccat tcacttttaa ttcttcatta ggactatgct tattctccat
    11461 tcacttttaa ttcttcatta aaaatgatgc ttattctcca ttcactttta attcttcatt
    11521 aaaaatgatg cttattctcc attcactttt gattcttcat taaaaatgat gcttattctc
    11581 tgttcacttt taattcttta ttaaaactga tgcttattct ccattcactt tcaattcttc
    11641 attaaaactg atgcttgcac tctgtaaact cataaacatc cattaatgct tcaccaaatc
    11701 tttgatagtg aactacttcc ctctcaccca aaaatctcaa tatatctttc aaatcagggt
    11761 catcagttaa atttattaat tgatagtacg tagccaatgc tttttgttca gcagccatat
    11821 cctcatgtaa atcagttacc gaatcgccaa agacattaat ataacttgta gtaaagttta
    11881 ctccattaga atctactggc tgaagaccat gtccaaaatt agcataatta ctaccaagtc
    11941 cagcagcctt aagttcttct ggagtacaat tgcctattaa ttgatataac actgaagaaa
    12001 ttatctcaac atgagccatt tcctctgtac ctatatcagt taaaagtgca cgagattttc
    12061 cagttggcat agtatatctt tgttgaagat atctagttgc agctgccaac tcaccattag
    12121 gtcctcccaa ctgagtcatg atatattttg ccattctagg gtctgcttgt cttatgttaa
    12181 ctggatgttc cagtgttttt tgataaatcc acatattatc ttactcctcc ttaatatata
    12241 tttaataata aatctaattt taaagtttaa taattttatt tataaaatga tttgtcccat
    12301 ggccaaggtt cagatatcca tgaccaagga tattcacttg attcatatcc aaaattagtt
    12361 aatgctccat atttagattc ataatcacac acagcttgtg taaatttatc gcagtatctg
    12421 ttgtatgaat caagagcttt cttatccctt ggattgttat ctaaatataa atttaattct
    12481 atacaggcaa attgatattc tgaaattctc tctaaaagtt ctcttctatt taatgacatt
    12541 agtacatgtt accccctttt ttagctctaa ctctattttt aggatttgca tatagccaat
    12601 cattatagtt atttgactct attaaatcta attcaggaaa taaagtacct ctttttaatg
    12661 ctgtttttgg attgtatgtg tcatcataaa tttgacgatt gatgtatggt ctagcaaatt
    12721 cacaacaata gtcttgttta tcctctttta aatatcccat taattatcct ccttaatttt
    12781 tagaatttta aaatagcatt atttttaaaa attgtatact ggttctatat tataactggt
    12841 agttttaata ggtctttaat aagattaaat gattaaataa gtatcttttt ttcttatttt
    12901 acctctaaaa atttttctaa ttagtctttc ataaatatct attttaaata aataatatag
    12961 agtttataca attt
//
LOCUS       DAELNY010000108        12934 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.108, whole genome shotgun sequence.
ACCESSION   DAELNY010000108 DAELNY010000000
VERSION     DAELNY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.108"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..764
                     /locus_tag="KPE55_002479"
     CDS             <1..764
                     /locus_tag="KPE55_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0493534.1"
                     /translation="NIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGRYYV
                     SLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELS
                     RKTIGSLNRNKARLEVAGLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNMIRN
                     RKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNI
                     REWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            1319..2725
                     /locus_tag="KPE55_002480"
     CDS             1319..2725
                     /locus_tag="KPE55_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0493535.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            2753..3601
                     /locus_tag="KPE55_002481"
     CDS             2753..3601
                     /locus_tag="KPE55_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0493536.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            4262..4954
                     /locus_tag="KPE55_002482"
     CDS             4262..4954
                     /locus_tag="KPE55_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493537.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLAELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4951..5886
                     /locus_tag="KPE55_002483"
     CDS             4951..5886
                     /locus_tag="KPE55_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493538.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            6034..6957
                     /locus_tag="KPE55_002484"
     CDS             6034..6957
                     /locus_tag="KPE55_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493539.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6941..7711
                     /locus_tag="KPE55_002485"
     CDS             6941..7711
                     /locus_tag="KPE55_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493540.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            7721..8503
                     /locus_tag="KPE55_002486"
     CDS             7721..8503
                     /locus_tag="KPE55_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493541.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            8879..9415
                     /locus_tag="KPE55_002487"
     CDS             8879..9415
                     /locus_tag="KPE55_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0493542.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            9447..9836
                     /locus_tag="KPE55_002488"
     CDS             9447..9836
                     /locus_tag="KPE55_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0493543.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            9873..10259
                     /locus_tag="KPE55_002489"
     CDS             9873..10259
                     /locus_tag="KPE55_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG0493544.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            10386..11828
                     /locus_tag="KPE55_002490"
     CDS             10386..11828
                     /locus_tag="KPE55_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0493545.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFART
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            12125..>12934
                     /locus_tag="KPE55_002491"
     CDS             12125..>12934
                     /locus_tag="KPE55_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493546.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTIELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCRDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTIS"
BASE COUNT         4716 a         1593 c         2448 g         4177 t
ORIGIN      
        1 gaaacattat gtattgtggt caacatataa aactacctaa acttggtatg gttaagataa
       61 gggataaaca agtacctaaa ggtagaatac ttaatgctac tatatctaaa gagccaagtg
      121 gtagatatta tgtatcatta tgctgtactg atgtagatat tgaagcattt gaaaatacta
      181 ataatcatat aggtttagat ttaggaatta aagaattttg tatctcaagt tgtggagaat
      241 ttatagaaaa tcctaagtat cttaagaagt cattaaacaa gcttgctaaa ttacaaagag
      301 aattatcgag aaaaacaatc ggtagtttaa atagaaataa agcaagatta gaagttgcag
      361 gacttcaaga acatatagct aatcaaagaa aggatttttt acaaaaacta tctactaaat
      421 taataaaaga gaatgatatt atttgcatag aagatttaca agtaaagaat atgattagaa
      481 atcgtaaact atctcgctta atttctgatg tgtcatggtc agagtttatt cgtcaattag
      541 aatacaaggc taattggcat ggtagacaaa ttgtaaaggt gggtaaattc tttgcaagtt
      601 cacaaatatg caataaatgt gggtataaaa acgaggaagt taaggattta aatataagag
      661 aatggatttg tcctagttgt aatgaaactc acgatagaga tataaatgca agtataaata
      721 tattaaaaga aggattaaga ctaataacaa ctcaaaataa ataacttatg taagaaccgt
      781 aggaactacg gggatagcct gtggagaatt agtaagacat attttagtat gcaaaattct
      841 gtgaagcagg aaccctgtga ctttagtcat gggaggttca gtttgggcaa gtagtttctt
      901 gtaaatctag ttatgtttca accaatgata atataatttc attagtatta gctggtaatt
      961 aagttaattt gactagagat aagtaaattg atttaaaagg aaataagtaa atcaacaatt
     1021 aaatacatct aaaagtaagt ttattgcaat gaattgttaa agaaaataat taatatggag
     1081 taaatctatt gcaatgaagt aacaaagaaa ataattaata tagagtaagt ttattgtaat
     1141 gaattgttaa agaaaagagt taatatagag taagtttatt gtaatgaatt gttaaagaaa
     1201 ataattaata tagggtaaat ctatctcaat gaaataacaa agaaaagagt taatatagag
     1261 tgaacttatt gcaatgaatc aacaaagaaa ataatcaata taaaaaggag aaatatttat
     1321 gaatacagga tttccaaaag actttttatg gggagcatca tcttctgcat ttcaagtgga
     1381 aggagcttgg gataaagaca ataaaggaaa aaccgtagcc gactataatt cttttaaaaa
     1441 gtctcattta caggcagata ctaaagtcgc atcagacttt taccataatt atgaagaaga
     1501 tattgaatta atgaaagaat tagggatgaa aacatatcgt ttttcaattt catgggcaag
     1561 aataatacca gatggtgaag gagagataaa ccagaaaggt ttagactttt ataataagat
     1621 aatagacaaa ctaatagagt gtgatattga gccattcgta actttatacc attttgacct
     1681 tccatttaaa ttagtagaga aatataatgg ttgggaaagt agagaaactg tctatgcttt
     1741 tgaaagattt gctaaaatat gctttaagca ttttggagat agggtaaagt attggcaacc
     1801 tcataatgaa cagaatttaa ttgttagagt ggaggagaga attaatatat atgatgaaac
     1861 agattcatgg aagatagata aaatgcgtgc tcagatggat tataatcttt gcttagcaca
     1921 tgcattagca gtcaatgcat gtcatgagat gataaaagaa agtaaaattg gagcagcagt
     1981 atcatcatct gtaacatatc ctcttacttc taagccagaa gatgtatatg cagctagaat
     2041 gaatgataat tttaaagtgt actatatgct tgatatgcat cattatggag aatatccagg
     2101 gtattatatg aagtatcttg aaaaaagaaa tatagttcct cacatggaag atggggataa
     2161 agagatactt aaaaaagcta aaatggattt tatagctgta aattattata ggacaaattg
     2221 tgcagaagct ttacctgaag attcacaaca tccatttgga ttaagagaag gaactgttga
     2281 ttttagtatg tatggactat ttaaaatgtc aatgaatcct aacttagagg cctctgagta
     2341 tggagcagca atagacccat caggtcttag agttgcctta aatgaatatt ggcaaagata
     2401 tcacttacct gttataataa cagaaaatgg tctaggagct aaagatatat tagaagatgg
     2461 aaaaattcat gatgactatc gtatagacta tttaagaagt catataaatg catgtaaact
     2521 agctattgaa gatggtgtag agatgattgg atattgtcca tggtcattta cagacctttt
     2581 aagttcttct caaggtttta ataagcgtta tggacttgtt tatataaata gaactgacca
     2641 tgaagtttta gatttaaaaa gaatcaagaa agatagtttt tactggtata aagaagttat
     2701 agagaacaat ggaatagtaa aataaacata aatatatatt gagggtttta gtatgtacat
     2761 tacaaaggta ttaaataatt cgttgctact tgcaaaggat gataatgatg aagaaataat
     2821 attgatgggg aaaggcattg gccataatta taaggccggg tctgagttaa ataaaaaaga
     2881 tatagaaaag atttttgttc ttcatgatga aaatataaaa aagagcatta tacaattggc
     2941 aacagaaata gatgcagaat attttggtat agctcaaatg ataattgcat atggaataga
     3001 aaaatataac ttaaaactta tgaaccatat atatttggcc ttaactgacc acattgcctt
     3061 tgcagtgaga cgttttaaag aaggtataat gattgaaaat cactatttgt tcgaaatcaa
     3121 ggaatttaat ccaaaagagt atgatatagg aaaatatgct attactgttt ttaaggaggt
     3181 tcttggttta gacctaccag aagaagagat tggtaatata gcaggccatt ttataaatgc
     3241 acaacaagat aatccttata gtgatagaaa taagagaagt gctaaaattg taaatgctat
     3301 tttgcaaata gttcattatc acttttctat tgtatataat aaagagtcat tttattatag
     3361 gagatttgta atgcatctaa aggccttttc acaaagattt ctggctaatg aaccatcaag
     3421 agaaaaaata gattttatat atgagcaagt tcaaaaaaat tgtaaggaag agtatgaatg
     3481 tgtgaaaaag gtaggagctt atatgtataa ggaatactcc agagaattac caagacaaga
     3541 ggagttatat ttgatgattc atattcataa gattttagga gaattatgcg aaaatagtta
     3601 gaatatctta atatgctagt aatattataa aaaattaaaa attaactaca ctttaaatat
     3661 gaataattgc attacttaag ggggttgtct taaaattgat ttttttaatt aaatttgaag
     3721 ctcaactcct tttttaaacc tttatatcat tacaacttta atgtttatac gcctgtactt
     3781 cagatgtata tctacttttt acatttctca acatgcaaat ctaccatttt ttgcagtatg
     3841 ttaattgaaa taaaccattg agttaaccct aaatttattc tggacttata ggaatagttc
     3901 caaagatact ttaaaatatt ttctggaata attgcttatt tcacatatgt tttattgtga
     3961 tgttttgatt taataaattc ttcaactctg tatatagtaa atgtattatc atgtttgttt
     4021 tattcaagca cacttataat acgcttgatt ttttcacaat gatgaaatat actaatgggt
     4081 gttataagta taagaatagc attacacttg aagaagcaag tgataaatga ggtataaatc
     4141 ctcatatgat aaactactat ttcattgagg agcatattct aaatgatgta aagatactac
     4201 aatatgacta aattcctaat gataaaaaag tctacgaatg agtgtagaaa ggggaactaa
     4261 gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt taaaaaagag
     4321 tatctcaatt gaacagattg aagcaaacta ttgccattct gggagagatg gactggctga
     4381 actttcaaaa caagattatc agttagtagt tctagacgtg atgatgccag gtatggatgg
     4441 ttttgaaact atggaacaaa tacgaaaata tagcagtatt cccatcttaa tgctgacatc
     4501 aaaaagcgat aattacagca aagtgcatgg attgcgagct ggtgcagatg actatctaac
     4561 caaaccattt gaaattacag aatttactgc tcgtgtactg tctttaatcc gacgttacac
     4621 ccgttttaat ccaaaaaatg aggagccaca gcaactttct ttcaaaggtt tgttgattga
     4681 ccttgaccag catacagtga ctaccaatgg cgaacctatg ttgcttccac ccaaggaatt
     4741 tggtgtgctt ctttactgtg caaagcatca gggtaagatt ttgacaaaac agcagattta
     4801 tgaagccgtg tggggtgagc catatgtata tgatgacagc aatatcatgg ctattatcag
     4861 tcgcctaaga aaaaaaattg aactagaccc atcatctccc atctatttgc aaacaataaa
     4921 aggaattggg tatcaattca acacggaggt atgaaaatgg acgaacaaac gataattctt
     4981 ttcttactct tgctgtcgat tggtctactt tttctttcca tatttgcttt ttccaagctt
     5041 gggcatattt acaagcgcct aaaagatata gaggagatac tggctgatgt acaaattgga
     5101 gacgaaaatc gtaaaatact aattaagcca tgtgatgtta tggctccatt ggtttatcag
     5161 cttaatgaga ttgtctacga ttatgaaaat aagttacttt ctctgaaaaa gtcggataaa
     5221 gctagcaaac aacttatgac aagtttatct catgatgttc gtacaccatt aacaactctg
     5281 attggttatc tggatgctgt acacagtggt atagttatcg aacaagaacg agaggaatac
     5341 cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt acattgatgt tctttttgac
     5401 tggtttcggt tgaactctga tgaatttacg ctgtcaatag aatctgtaga aattgcagag
     5461 ttgtcacgca atattttgaa agactggatt cctatattcc atgaaaagaa actgaatttt
     5521 gaaattgata ttccagaaaa tcgactaatg gtaaacctcg acccagatgg atattcccgt
     5581 gtggtcaata atctggttca aaatgttctt gctcacagta aggctcggca aatcaagatt
     5641 accatgagtg aggattccag aatggtatta ctccgtgttg aggatgatgg tgttgggatt
     5701 gccagagaaa atttaccaca tatttttgag cgactgtata agtgtgataa aggacgttca
     5761 gaaaaaggaa gtggtcttgg tctatctatt gtcagtcaaa tggtggagcg aatgggtggt
     5821 cagatttccg tagaaagtga gattggaaag tataccatat ttacagtgag cttaccatta
     5881 atataaaatt gtttttcccc catgtgacat gtatacacag ggggattttt tgtaagttat
     5941 aaagttgcaa ggttaataca aggttgttgc aaggttaatg taagattgag gtggtatgat
     6001 aactttaata aacaaaggag gcttttatta tttatgaata atttaattat tgaaacaaaa
     6061 aatctcacta agcaatatgg tgctcaaaag agcgtttcta atttgaatat tcacgttcag
     6121 aaaggtcgca tttacggatt gctaggaaga aacggtgcgg gtaagacgac aactatgaaa
     6181 atgttattgg gtctaacaaa accaactact ggagaaatta aaatatttgg tcaggacatt
     6241 cacaataacg agaagaagct tcttcctcga attggatgtt tgattgaatc accagggttt
     6301 tatcctaatc tgacaggaac agaaaatctc aaaatctttg caaggcttag aggaatacca
     6361 aaaaaaaatg cagtagaatc agcactggat gtagtcggtt tgccaaacaa tgacaaaaag
     6421 ttattttctc aatactctct tggtatgaaa cagaggttgg cgattgctct tgccattatg
     6481 catgacccac aactgttgat tttggacgag cctatcaatg ggcttgatcc tattggtatt
     6541 gcagaagtaa gaacatttat tcgtgatctc tgcacagagc gaggtaaaac tattttgatt
     6601 tctagtcaca ttctttctga aattgccttg ttggcagatg acattggcat tattgaccat
     6661 ggtattttgt tggaggaaga aagtcttgcc caattagaag aaaaaaacag taaatacata
     6721 cattttgctg tatctgatgc agcacaggca gcaaggcttc tggaaaccca acttaacctg
     6781 aaaaatttca aagtagcaga taattacaat attatgctat atgatacaga tgtttctgtt
     6841 gcagaaataa atcgtgcttt cattctaaac aacattgagg tatcagaatc tcatttatgc
     6901 gaagatactc ttgaagatta cttcaagaaa gtaacagggg gtgaaggaat tgcttgatat
     6961 aatttcatgt gaattttcaa aactcaagcg gttaaaattt atactgattt ctattttagg
     7021 tgcttgctta tttccaattc ccgcaacaat tttgattgca aaggataact tgccctttga
     7081 gcaattgttc aaattagttg tcaattttgg atattttttg ctattaccaa ttgtattaag
     7141 tattgttgct agccaactat tttttataga aagagataat gatgtattaa aaaatcttgc
     7201 aacagtgcca gtgcaaaaag gaaaactggc tctggcaaaa ttggctgtgt tgctgtttat
     7261 atcattgttc tactctgttg cagggttagg agctactatc attggaggtt tcattgttgg
     7321 aatagtagag ggggttgctg taaaattagg aatgagcatt gcttttggaa ttatgttttt
     7381 tgttactgta cttcctgttg tggtactcat tgtatatttc aatcgcagct atattttttc
     7441 tattatactt tcttttgtat atgctatttt caattttagt atatccttaa atattatcaa
     7501 ttttgaacca aataacccaa tattaagtgt attacctgct cctgttataa tgcgttggtg
     7561 gatggcattt tggggagatc ccactggcga atacacggca cttagacagc catatttgct
     7621 ctcaactcct gcttgtgtag gtattttatg tttgataacc attatagcag tcctgctgat
     7681 ttgtatgata tacaaaaagc aggagaatta ggaggtatct atgcttgata taattactac
     7741 agaatttcaa aaaataaaaa gatataatat tctatggatt ggtgttgttg ctgttctgtt
     7801 ttcagcacta ttggcagttt ttcaggttgt aagttcacac ggcagtgacc ctctaacgta
     7861 tgagagcttt gcaaacggag ttatctggaa taatttcagt cttagttttc catttgggat
     7921 tacacttatt ggaggatatc ttattaaccg tgaatataca gaccaaacct taaaaaacat
     7981 cctaactgtt cctatttcat tacgcaaatt gctagtggga aaattaattg cggttggagg
     8041 aattactgta ttgttaagtc ttttcagctt cttatgcaca tttattttgg ggttgttatt
     8101 ttgcaatatt aatatatctg ttacactcgt tgtaaaatcc ttagttcaga ttttgtctgt
     8161 taatctgtac tgttatgttg ctgttttgcc gattattgct tattttggtc gtaaacaaaa
     8221 tgcatttctg acgggtgtag ggattgcatt tgtatacggt ttttgtggtg tgtttgttgc
     8281 tggaagaaat ctgacagact tttatccaat cacagcaggt cttggtctag ttggatacaa
     8341 caatggtgct ggtactgttt atcagccact tattggtgct atgactttga ttgtaattct
     8401 aatcttgaca actattctac ttgtatttac acctaattat gataaagtta tgaccatatc
     8461 taagaaaaaa gggataaagc ataaaaagta ttctcataaa taacataaat attgtttcta
     8521 ttatttaagt tgctagacag tgaaaagaaa aaatctgtcg tacagtggct atatgctcat
     8581 gtattcaaga agctacagtt ttgatttttc ataactgtag cttctttttg tatttattaa
     8641 atcacgacta tcataatact tgcaataggt atagcgacaa gagagagtac tgtcatatgt
     8701 gttttatata ttacattaag tatattctaa aatatatgtt ttaaataata tatttgaaaa
     8761 taggaatatt taaataaaaa aaataatatt atatatagtt gacaaaaatt ggcaaatgat
     8821 ataatatata aatatagaaa tagtaatcat tactattatt aaaagggagg aattaattat
     8881 gaacttaaaa ggaactaaaa cagaaaagaa tttaatggca ggatttgcag gagagtcaga
     8941 agcaagaaat aaatatacat actacgcttc aaaagcaaga aaagaaggtt ataatcaaat
     9001 agctgctata tttgaagaaa ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa
     9061 acttttacat gatggtatgc cttcaacaga ggaaaattta aaggatgcag cagcaggtga
     9121 aaactatgaa tggactgata tgtatgcaaa atttgctaaa gaagctagag aagaaggatt
     9181 tgacaagata gcttatttat ttgaagctgt tggaaaaata gagaaagagc atgaagaaag
     9241 atacttaaag cttttagaaa acttaaatga aggaaaaata tttaaaagag atgaagaagt
     9301 tgtttggcaa tgtcaaaact gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg
     9361 cccagtatgt gaccatccaa aagcatattt taatataaaa gctgaaaatt attaaaaatt
     9421 atcattatta aaaaggaatg ttaactatga aattttctaa acaacgagaa ctgattttaa
     9481 atgaaatatt aaataatccg gttcatccta ctgcggatta cctatatgaa aacttaaaaa
     9541 aagataatcc aaatttaagc ttaggaactg tgtatagaaa tttagctcag ctaacggaac
     9601 atggctttat aagaaaggtt agtattccag gatatccaga tagatttgat ggtcgaatag
     9661 ataatcacta tcatatcata tgtgaagtat gtggagaggt atacgattta gaatccgaag
     9721 ttcttaataa tttacaagaa ttaatatctg aggagacaga cataaaaata acatcttata
     9781 acataagttt taaagggatt tgtaataatt gtaaaaggtg tagccaagta ggttagtttt
     9841 caaattctaa aaataattaa tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat
     9901 gtgagacttg tggaaacctt gtaggaatga tacaaagtgg tggtgtacct attttctgtt
     9961 gtggaaaacc tatgaaggaa cttgttccta atacaacaga tgcagcagtt gaaaaacatg
    10021 tacctgtaat tgaagtggat ggaaataatg ttactgttaa agtaagtagc acaactcatc
    10081 caatgactaa agaacatcat atagcatggg tgtatcttat gactgaacaa ggtggtcaac
    10141 gcaaatgtct agcagtagat ggagaacctg tagttaaatt tgctttgaat gatgatgata
    10201 aagtgatttc tgcctatgct tactgtaatt tacatggatt atggaaggct gaactgtaat
    10261 atttaaataa attgagtcaa aaaggaggct ataaatgtat tttcatacaa atagcctcct
    10321 ataaatttat ataaattcaa gttttaattt tgtttaatgt caatataata taaggagaaa
    10381 ataatatgtc aatttataaa tgtagtgttt gtggatatat ttatgatgaa tctaagaatg
    10441 ataaaacatg ggatgaatta agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta
    10501 gttattttgg aaagataagt actgtttact atgaagaaga tgaaaagata gcagaagaca
    10561 tagttgaaga tgaatctaaa ttaaatactg aaaaagaggg agatttaaat tacttaagta
    10621 catatttaag aagagatgat gaagttgaga aacatatgga tataattcac gaaatggctg
    10681 ttacaggaaa atctataata gaacctatga gaactaaact accagtaatt tcttgggatg
    10741 atattttaat tatgggtgct caattaaatc cattaccttt aaatgagcat gatgaagtaa
    10801 atacaacaac aataatagga aaaaaagcaa agaaacctat gattatagaa aatccagttt
    10861 atatttccca catgtcattt ggagctttgt ctaaagaact aaaaatagct ttagcaaagg
    10921 gtgcagccca aaataagaca gctatgtgta gtggtgaagg aggtatatta ccagaagaga
    10981 aggaagcttc atacaagtat atttttgagt atgtaccaaa taaatatagt gtaactgaag
    11041 aaaatcttaa aaattctgat gctatagaga taaaaattgg tcaaggaaca aaacctggta
    11101 tgggaggtca tcttcctggc gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc
    11161 ctgtaggtca agatgtaata agtccatctt gttttgagga aatacaatcc aaagaggatt
    11221 taaagaaact tgtagatgaa cttagagaag tttcagaagg gcgtccaata ggtgttaaaa
    11281 tttcagcagg tcatattgaa aaagatatgg aatttatagc ttatgctaaa cctgattttg
    11341 taactattga tggtcgtggt ggagctactg gagcaagtcc aaagttgctt aaagatgcaa
    11401 cttcaattcc aacaatattt gctctttatc gtgctagaaa atacatagat acacatggat
    11461 tagatataga tttggttata acaggtgggc ttcgtatatc tactgatttt gctaaggcaa
    11521 ttgctatggg ggctgatgca gttgctattg caagttctgc tcttatggca gcggcgtgtc
    11581 aacaatatcg tatatgtggt tctggtaagt gtcctgtagg agtagctact caggatgagg
    11641 aattgagaaa aagattacat atagaaaaca gtgctaatag agtggcaaac tttttaaatg
    11701 taagtttaga agaacttaag acttttgcta gaacttctgg tcataaagat atacatgatt
    11761 taagtgttga tgatttatat actgttaact ctgaaatatc aaattataca aacatacagc
    11821 atgtatagat tgaagtttga aactattaga ttttagatag ctataaaagt aaatgataaa
    11881 tatattttat aaaagagagt gtcttaaaat gaacttttta gttcataagt cactcttttt
    11941 tttatgaagc caaatatatt tatcatttta gcaatgaaaa agaatcttat ctctttattt
    12001 tgagatagat tccttctatt tatgaaaatt aaagatatta aattgtaaaa tattctgtga
    12061 tgtcgtacta ttaaactaaa gattctgtac tttaaagtaa ataaaaattt aaggagtgat
    12121 tattatggaa agattgaaga tacaagtttt agtcgacaat aatacttata ttgatagata
    12181 ctttgttgga gaaccagcag taagctatta tattgagatt gatggaaata ggattttgtt
    12241 tgactcaggg tattcagatg tattcatatc aaacgctgaa aaattaaata tagatttagg
    12301 aaatttaact catgttgttt tttctcatgg gcataatgac catacaagag gtatccagtt
    12361 tttagaaaat aggtatgatt tatctacaat tgaacttatt tcacatccta actgttttat
    12421 acctagaaaa catggagaga aaagcatagg agctccattt tctgctgagg aaattaaaaa
    12481 tatttttatg tataatccta aggataagcc atttaatcta agcaagaatt gtgtgttttt
    12541 aggtgaaata ccttctataa atgattttga aaaaagagct aaaattggta aatgtaaaat
    12601 aggagatttg tgggaagatg attatgtttt agatgattct gctatagttt gtaaaactga
    12661 taaaggtatt tttattatta caggatgttc tcatagtgga atttgtaata tagtagagta
    12721 tgctaaaaag gtatgtagag atgatagagt tattggtata ctaggtggtt ttcatctttt
    12781 tgaattgaat aataggttag atagtacaat acaatatttg gataaagaag aaataagaat
    12841 gttgtaccct tgtcactgtg tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa
    12901 tataaatgaa gttggagtag ggctgaccat aagt
//
LOCUS       DAELNY010000109        12776 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.109, whole genome shotgun sequence.
ACCESSION   DAELNY010000109 DAELNY010000000
VERSION     DAELNY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.109"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            138..539
                     /locus_tag="KPE55_002492"
     CDS             138..539
                     /locus_tag="KPE55_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493547.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            582..875
                     /locus_tag="KPE55_002493"
     CDS             582..875
                     /locus_tag="KPE55_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG0493548.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            890..1330
                     /locus_tag="KPE55_002494"
     CDS             890..1330
                     /locus_tag="KPE55_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG0493549.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(1422..1895)
                     /locus_tag="KPE55_002495"
     CDS             complement(1422..1895)
                     /locus_tag="KPE55_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0493550.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(1895..2683)
                     /locus_tag="KPE55_002496"
     CDS             complement(1895..2683)
                     /locus_tag="KPE55_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0493551.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            3011..4255
                     /locus_tag="KPE55_002497"
     CDS             3011..4255
                     /locus_tag="KPE55_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0493552.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            4578..5675
                     /locus_tag="KPE55_002498"
     CDS             4578..5675
                     /locus_tag="KPE55_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0493553.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            5666..6364
                     /locus_tag="KPE55_002499"
     CDS             5666..6364
                     /locus_tag="KPE55_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0493554.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKKVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            6367..7431
                     /locus_tag="KPE55_002500"
     CDS             6367..7431
                     /locus_tag="KPE55_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0493555.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFVMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            7614..8318
                     /locus_tag="KPE55_002501"
     CDS             7614..8318
                     /locus_tag="KPE55_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493556.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            8311..9633
                     /locus_tag="KPE55_002502"
     CDS             8311..9633
                     /locus_tag="KPE55_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493557.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            9820..11193
                     /locus_tag="KPE55_002503"
     CDS             9820..11193
                     /locus_tag="KPE55_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493558.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(11565..12503)
                     /gene="pdaA"
                     /locus_tag="KPE55_002504"
     CDS             complement(11565..12503)
                     /gene="pdaA"
                     /locus_tag="KPE55_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0493559.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDDNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTT
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         4802 a         1483 c         2141 g         4350 t
ORIGIN      
        1 gactacacat tgaagtaaaa gaagtagcta aaattattcc ttttattttt gttttttgta
       61 tctatttggt ataatagtat tagagcgtat aataatttag gcaaaatttt ttaaggaaga
      121 ctagaggaga atataaaatg aaaaaatgtc tttactgtaa tagggatata aagtcaccaa
      181 ataataaatt agctataaaa tatgatgaca atatgaatat atatccatgt cgtgcagaat
      241 gtaaggaaaa aattgaagaa tatatagcaa aaaaacctac atataagttt ataaatatat
      301 tagaggttct tttaggtgtg ggcggaatat tctgcttttt gagtaaatgg actattgcag
      361 cccaagttgt acttggaatt gatgcactgg ttatattttt attcccattg gcaggattta
      421 agatgaatgg aaagttaagt atggaacaaa ctaagaatca aagtaggtct attgcaatat
      481 caattttggc atttatagta ataataactg tattatattt taaacaagtt ggtaagtaga
      541 gcataattgt ataagacatc attagagtat gaggtgagtt tatggaagaa agaaaattag
      601 caccaaacaa tgagagggaa gctctcataa aaagattaaa aagaatagaa ggtcaagtta
      661 agggtataca aaagatggtg gaacaagaac gttattgtgt tgatatattg gttcaaatat
      721 ctgctataag gtctgctata aatagagtag gcacaattat cttagagaat catataaaag
      781 gatgtgtagc agaaagcatt aaacatgatg atgctgataa aactgaagag atgataaaag
      841 agcttatgga tactataaat aaatttacaa aataaggagg cggttagtta tgaatcaaca
      901 aggatttatt atggaaattg ttgatgatag aactgcaaag ttaaaaatgc aaagacattc
      961 agcttgtgct gcttgtggaa aatgtgctac tacaaattca gcagagtcaa aagaaatatt
     1021 agtagaagta gacaacacaa ttggtgcaaa agttggtgac catgtagaag tgagtatgga
     1081 taatatgaat gtattaaaag cagctgtaat ggcttatata atacctctta tagcacttct
     1141 tgtaggaaca atagggactt attatatatt gggggcaatt ggaatgacaa ctggagttga
     1201 agccataagt ggattagttg gaatttgtct tacaataatc tgttatttat acttaaagaa
     1261 gaatgacaaa aaatttagag acagtagaga atttatacca gtaataacaa gaatattaat
     1321 tgacctttaa gataattata attaaaaaat agcaatgtac tttttaaata aatactcaat
     1381 acgtatttgt agagtttaag tacattgcta tttttatatt tttattttga ttttctgtaa
     1441 ggaataaagt tttttctgat ttttaaaaga ttcaatgttc ttacaaaaga agttacaact
     1501 aatacaccaa gtgctattgc agcacttaga aatagagcat ctgtaccttt tgacatagcc
     1561 aaagaataat ttttctgaat caaagacaac attgtattat atagcccaat tcctggtatt
     1621 agaggaagaa tacctggaat tacgaacaca atagctggtt gctttagttt tcttgctaaa
     1681 gtctcactgc acgctgagac caaagcagca gcgataaaac ctgcaaaaac tgcatttgta
     1741 gtaaaattaa ataaataaac atatacaatc caaccaattc cgccagttac tccagctgga
     1801 agaagtagat atagtggagc attaaagaaa acagcaaatc cagcagttgc aacacctgaa
     1861 aataaaaaat gcaaaatcat agacatatca gtcattagaa aggacctcct attctatacc
     1921 atgtatcaag gacaaaacca actccagaag caattgcaat aataatcata gcagcatcaa
     1981 aagcacgaga aattcctgat attaaattcc ctgatattaa atctctaata gcttttataa
     2041 aagcaactcc tgggagtagt ggcattatag cgccgactat aagcatttta gggtcatcta
     2101 aaatgcctat ttcaactagt agaactcctg aaagtgcaat taaaaaagaa gatactaaac
     2161 aggaaaatgc agatattgca cttatcttca tcgttttatc ataagtaatt gcagcaaaga
     2221 ttgctattat aaacgtaagt ataaaattta atacataatt ccctccaagc aaacaagcaa
     2281 aacaagcaga tgcaattcct gtacaagagt aaaataacca taaaggataa ggttttacat
     2341 cttggatttc ataaagacgt gcaatggcat tatcaatatc atactctttg tcactaacaa
     2401 attctcgaga aaaactgttt aataaagaaa tcttatttaa gtttatacct cttgattgaa
     2461 tcgttttcat aaacgaaaag ccatcaaatt tctcatctga aataattatt actgtaggag
     2521 ttgtaaaaat atttacatga taaaaccctc ttgacctgca tattctaagg acactgtctt
     2581 caaccctaga tgtttcagct ccatttttta tcatcagttc ccctacaaat agagctagtt
     2641 ttagaatatt tttcttatat tcaagttcct cattttgatt cataaataca cctccaaatt
     2701 aaaaaacgta atattgaaat tatatcaaat ttttaagtaa tattccttaa tatctatata
     2761 aatatattat cttttttttg tttcaaaata gtcacattta aattatatcc aattaaaaga
     2821 attagacaat attatttatt atattttaac ttaaaaataa agaaagtaac tatgttaaat
     2881 atggaaaaat ggcaaaacac ttgactatta attggtaaag gactataata ataaattatc
     2941 atattgataa aaacgaaaat tatgaaatgt ctgtaaaaat aaaatttaaa taataactag
     3001 gaggagtaat atgtttgaga acttgaaaaa agcggaccct gagatttatg aaagtatgaa
     3061 aagagagtta aaaagacaac aaagaaatat tgaattaata gcatctgaaa acatagtatc
     3121 agttccagta atggaaacta tgggaagtca tcttacaaat aagtatgcag aaggttactc
     3181 aggcaaaaga tattatggtg gatgtgaata catagatgaa gttgaaactc ttgcaataga
     3241 tagaatcaaa aaaatatttg gagcagaaca tgctaatgtt caacctcact ctggagcaaa
     3301 tgcaaatatt ggtgtatatt ttgctatgtt aaaaccaggt gatgttgtta tgggaatgaa
     3361 tttatctcaa ggtggacatt taactcatgg tgctccagtt aatatttctg gtcaatatta
     3421 taaattttat gaatatggta tagataaaga cagtggactt atagattttg atgaagttag
     3481 aaagatagca catgaagtaa aacctaaaat gatagttgca ggagcaagtg catatcctag
     3541 agaaatagat tttaaaaagt ttagagagat agctgatgag gttggagcat tgcttatggt
     3601 agatatggct catatagcag gtcttgtggc tgctggtctt caccaaaatc catgtgaagt
     3661 ggctgatttt gttacaacaa caactcacaa aactttaaga ggacctcgtg ggggtgttat
     3721 tctttgtaaa gaaaaatatg ctaaagacat agacaaagca atattccctg gtattcaagg
     3781 tggacctctt gaacatataa ttgcttctaa ggcagtttgc tttaaagaag ctttaagtga
     3841 tgagtttaaa gaatatcagg tacaagttgc taaaaatgca aaagccttag ctgaagaact
     3901 tattaaaaga gattttaaat tgatttctgg aggtacagat aatcatttaa tactattaga
     3961 tttgactaat aaaaatataa ctggtaaagc agcagaaaag agattggatg atgcttatat
     4021 aacagctaat aaaaatacaa ttccatttga cccaaatggt gctcttgtta caagtggaat
     4081 aagacttggt actccagctg taactactag aggtatgaaa gaagaagata tggcaataat
     4141 cgctgaggct atagacctat gtttaactta tgatgaagaa tctaaggcta gaacattggt
     4201 tgttggttta actgaaaaat atcctttata tgaagaatat aatattatgg attaattata
     4261 aagaacaact aaataaaata catttaaata attacaatga ttgtgtatgt atttgaatat
     4321 atttagatat aaaaaaggtg tctcaaaatg aactttttag tttatgaggc acttttttgt
     4381 atggaatcga acatatttgt attatttaaa taataaaaaa tatggttatc tctattttta
     4441 gatggtctct ttgtattttt taattaaaac tttaagattt cttaagaatt atactaggtt
     4501 tttctaaaga tttaaagtct atacttataa gtatagtaat catatgaaaa gaaaagcttt
     4561 aacgaggagg gaatcttatg aaggtattaa ttcttacagg aaaatttggt atgggtcatt
     4621 actcagcatc aaattcttta agtgaagata taaaagcaaa atttgataat tcagaaataa
     4681 taattaaaga tatatttgag tacattatgc ctaactattc agataaaatg tacaagactt
     4741 tttctattct tgtaaatcgg ggaagtagtc tttataattt attttataaa tgtgctgaaa
     4801 atggaaagaa agatataaaa tttacttttt cagattattt tttaaataaa ttagatacat
     4861 tacttcatga agtacaacca acagtagtta tttcaacttt tccattttgt tctcaattag
     4921 tatcaagata taaggaaaaa tataactcaa atcttccttt aataacatgt ataactgata
     4981 taagtagtca ctcagaatgg attagtaaaa atacagactg ttatttagta gctagtaaat
     5041 ctactaagga agaacttgtt tttaaaggaa tagatgaatc taagattaaa gttaatggta
     5101 ttccagtaaa aaaagaattt aaaagaatag aacatgtaaa tcattcaact aaaaaaaata
     5161 ttcttataat gggtggagga ttaggattac tgcctaaatc agaacaattt tacaaagaat
     5221 taaatagttt agaaggagta aaaactactg taataactgg taataataaa aaaatgtact
     5281 ataaactata tggtagatat gaaaatatag aggtagtagg ttacaccaat gaagtatata
     5341 aatatatgaa agattctgac cttattattt ctaaaccagg tggaataaca ctatttgaaa
     5401 caatttattc agaacttcca atattggcat ttaatccatt tttacaacaa gagatagata
     5461 atgctagttt tatattaaat aatgaaattg ggagaatact tggtaaaaat aaaaaatact
     5521 atgtagatga gataaaagat ttaatttatg atgatgctac tcttaaagaa atgagcagta
     5581 acatgaaaga attaaaaaaa caatttgata ataatacctt agagaatatt ttattttcac
     5641 ttgatgaaca aggagcgtgt agagaatgta tgtagtaggt ttaattatcg ttgttgcatt
     5701 gattttttta gtccattcaa ttatacctac atactataat aaactattaa ataaaaaggt
     5761 attaaaaaat atggctggag aaaatgaaat tgcacttaca tttgatgatg gtccagataa
     5821 gagatataca gaaaaattac tagatgtgtt aaaagaaaat gatatacaag ctatgttttt
     5881 tgtagtagca aagaatgctg aaaaagagcc tgaaattata aaaagaatgt tgagagaaaa
     5941 tcatatagtt ggtttacatt cattggaaca cagaaatgca tggctttata gttatagtta
     6001 tgttaaaaaa gattttattg aaagtacaaa tataatgaaa aatttaggtg tagatgtaaa
     6061 ctactatcgt ccaccatggg gtcatactaa tatattttca aattcatttg ttaaaaaata
     6121 taatctaaaa atgactttgt gggatgtaat ggcagaagat tgggagaaag atagtacagt
     6181 agacattatt ataaacaagt taatgagtag aacaaaagaa aattctataa tttgtttaca
     6241 tgatgcaggt gaaaattcag gtggtgcagt aggtgcacct gagcgtacta ttgaagcttt
     6301 aaagatagct attccaaaac ttaaagccag tggtttaaag ttcgtaacac cagaaaggat
     6361 gtagttatga taagtaaaac tgaaaagaaa aataaatgcc taggtagtgt agctttttta
     6421 gttttactaa tgggcataac aggatatttt gtttttagag ggcagtcagt tgaatcattg
     6481 ataaaatcat taaaaggtgc aagccctatg tttatactaa ttggatttgt aatgatgttt
     6541 atatatgttg cctgtgaagg tataaatata tacttaggta tgaaagcttt aaaccaaaag
     6601 actactcttt taaaatgtat gggatatgct tttattggat tttattttag ttctattaca
     6661 ccttcagcat caggtggtca accagcacaa gtttattata tgaaaaaaga cgatataaat
     6721 atatcatatt cttcacttat actattagtc attgttgtta ttcaccaagt agttatactg
     6781 gcatatagtg gaataatgtt cattatggaa cgtgagttta tattaaataa tgtaagtggt
     6841 atgaacattt tactaatata tggtgtaata acaaatgttg ctcttgtcat tggagtaatt
     6901 gcaattatat tttcaaaaaa acttgtaaac aattttataa tatcaataac aaatttatta
     6961 ggtaagttaa gaataataaa agatgttgaa agttcaagaa aagttataat atctcaaatt
     7021 gaagagtatg ttaaaggtgc tcaatatata aagcagaatc caaaacttgt agtccaaatt
     7081 ttagtcataa ctattgtaca aataacagct atgtttttag ttcctttctt tgtatacaaa
     7141 gcttttcatc tatcaacata tactgtattt gagatattag cgatacaatc cttgcttaat
     7201 attgctgttt cttcattacc attgccaggg gctgttggag cttctgaaaa tagttttatg
     7261 acattattta agatattttt ccctggtcac ttattagttc cggcaatgtt acttagtagg
     7321 gggataagtt tttatgcatt tgtagctata agtggattga tatgtatagt agtacatgta
     7381 aaatcttctg aaaaagtaaa agcagtgaaa aaagttgctt atgtaagata atattatata
     7441 gattattttt attaatatgt caatttgtgt gtatatttta attaagcttt aaacatgttt
     7501 acaggagtag aaactattaa aacgtgtgac attgctaagc ttttgctaat aaagtataat
     7561 attttatata gtttgaaata tttaagaaat atagcaaaaa aaggagcttg gtgatgaata
     7621 aagagattcc aaaaaaaatt ttagtagctg atgataatcc tgagattagg gaaatcgtag
     7681 aaatactttt aactggtgaa ggttatgaag ttattatggc aacgaatgga gaagaagctg
     7741 taaacttagt agatagtacg attgatttga ttattttgga tgttgtaatg cctgtaaaaa
     7801 caggatttgt tgcatgtaat gaaattagag aaaaaacgac agcaccaata ttatttttaa
     7861 ctgcaaaaac tcaagactct gataaagtta ttggattttc agcaggagga gatgactatt
     7921 tatcaaagcc tttttcatat tctgaactaa tttcaagggt taagtctcta cttagacgtt
     7981 attatatata ccagggaaag gaaaaggaaa gagagccaaa tatattaaaa acaaaagagt
     8041 tatgtgtcaa tttagatact caagatgtta cactaaatgg agaacctgtg tttttgacaa
     8101 ctatagaata tagtatatta attcttatgt tgaaaaatag aaaaaaagtt ttttcgtctg
     8161 aaaatttata tgaaactatt tgggatgagc agtattttta tacagcaaat aatacaataa
     8221 tggttcatat tagaaattta agaaaaaagt tagaaaaaga ccaaaaaaat ccacaatata
     8281 taaaaaccgc atggggaaag gggtattata ttgattaaca aaattaagcc atctaagttg
     8341 ggagttatac tattgatata tgtagtaatc tcagctgcag tggctttcat tttaaatata
     8401 ccattttact ctgctgttga atctatagta aaagatgtat cagaagggaa agtatatttt
     8461 aaaagagaag taaatcaact tatggataat tttcaaactt atgtgaataa caatggtata
     8521 agagcagatg atggagatga tatagcaaaa tggaatagtg ataattggtt tgtttctatg
     8581 aagatatata aaggaaataa ggttttttat gatacattat actatcctaa tgaagtacct
     8641 gataagagtg aggaaaatga gttttatagc agtcccatgg ctagaaacta tgagatacaa
     8701 ttttctgata ttaaagctaa tatagttgta gtagctttat ttagatttag atttcaaaat
     8761 tttatagaag caatttctat tacatttatg gcacttacat ttattataac atttttactg
     8821 ttgtttagta gaaagataaa gtacctaagg aaaatagaaa tgggtataaa gatagtagag
     8881 agtggcagtt taaagtatag aatcccagta aaaggtaatg atgaattatc atcacttgca
     8941 tctagtatta atgagatgag tcatacattg gagaatcaac ttataaaaga aagggagatg
     9001 aagaataaaa ataaagatat agtaacttcg atatcacatg atgttagaac tccattaacg
     9061 tcagtaattt gttatttaga tttgattaaa gaccataaat acaatagtcc tgagcaattg
     9121 gaacaataca taaaaaatgt aaggtctaaa gcatatcaaa taaaagattt aacagatgac
     9181 ctttttacgc attttgttaa ttctggagag gaagtttcaa aagggtatga aaaaataaat
     9241 gctaatgaat ttatttttca attactttta gatagttcat atacattgga ggagaaagga
     9301 tttcatataa caatagaaaa tgaactttat aaccaatatc atatttatgt agatgttaca
     9361 caatttagaa ggatatttga taatttatgt tctaatataa taaaatatgc taggaaagat
     9421 aagcctatag tatttttaat taaagtagag gattctaaat tactaattat acaaaaaaat
     9481 agagtaagta atttttctaa gaatgtagaa agttttggaa ttggtataaa gaattgtgag
     9541 aaaatagttg catatcataa tggaagcata aatgtaaatt cagatgataa agaatttgct
     9601 attgaaatat ctattccagc aaaagaatca taaaaattta aattatatat tgtgtaattg
     9661 ctattatata ttgtgtaatt gcttatgtaa aattctatga agcaggaagc atgtgacttt
     9721 agttatggga tgttcaatat tgtgtacctc taggcaagta acaagtttac ttataagacc
     9781 atataatatt attatcttaa aaaaagggga cataataata tggttttgac aggagcacat
     9841 tatatttaca ttctatttat ggtaattata ttaattacta tgattatgaa aaaggataca
     9901 atagtaccat gtatattagg tgtttttttt atggggttgt tctttgaaaa aaacattttt
     9961 ggtgctataa cagcagtgtt taactctttt attatatctt taaatgagtt gggtccaatt
    10021 atcttgatta tagctattat ggttgcatta tctaaagccc tagaagccaa taatgctata
    10081 cagtatatgg tgagaccatt ttcgagagtt ataaaaaatt ctaatacagc attttttgta
    10141 actggatttg ttatgttagt tctttcttgg tttttctggc caactccagc tgttgcctta
    10201 gttggagctg tatttttacc tgttgctatg agagctgggc taccagcaat tggtgttgca
    10261 gtagcattaa atttatttgg tcatggtctt gccttatcta cagactttgt tattcaagga
    10321 gctccaagta taacagctgg tgcagcaggt gttgcagtat catatgttat aaatgatggt
    10381 atgattttgt tttgggtgat gggaatcgta accatatcta tggcatttta tacattaaaa
    10441 agagatatta acaaaggaat gtttagagaa gaactaaaag gttttgaaag tgaagaagta
    10501 gaagagttta atggaaaatc taaaatagca actattttag tagctctggg atttcttgct
    10561 gatataattg ctatgtatat atttgaccta aaaggaggag atgcatcagc tcttttgggt
    10621 ggaacagcag tatttttaat aataattata aatgttataa attttggcaa agatagtctt
    10681 gaaaatgtat gtgagaatat aattgatggt tttgtgtttg ggataaaaat atttggagca
    10741 attataccaa tagcagcatt tttctatatg ggtgaagttg caccattgac aggtgtattt
    10801 ggaaaagtat tagcaccagg ttcacaaggt ttattatcag atataggcat agctctttct
    10861 caaacaatac cacttaataa atttgccgta tcaggtatag aaactgtagt gggagcaata
    10921 actgggcttg atggttctgg attttctggg atttcattag ttggttcact tgcctctgta
    10981 tttggaactg ctataaatgc aagtgttggt gcactagctg cattaggaca aatcagtggt
    11041 atatgggtag gtggaggctg tctagttcct tggggtctta tttcagcagc ggcaatttgt
    11101 ggagttagcc caatagaatt ggcaaagcga aactttgtcc cagttataac cggattggtt
    11161 gtaacaacca tagtagcaat gtttattatt taataaatcg aataaatctt taattgtata
    11221 aattaaaaat agatgtgtag ctataattta ttataattgt tttactaatt ctaagccata
    11281 catctatttt tattgatatt tttattagta ttctttaata aaataatatt tttcattata
    11341 ctatacataa catctaaata taacatctaa aatactgtta tctatattta atatcttgcc
    11401 aaattttttg atgttttatt gtcctgtccc accaacatat ataagctatc acaagttttt
    11461 acctatacta tcaccaaaca aaaattctaa ttttaattta ttattttata acctatatta
    11521 tattaactat attagtttgt agactttaga tgtaattata aatattaata ctccaagagt
    11581 tcaaattcat atcctagttt tcttgcattt gatataaaat catttaaaat ttctgtacta
    11641 gttttggaaa ctgcatgtag taacaagata gaaccatcat gtagattatc cattattttt
    11701 tgcgttgctt cttcatgtga tggttgttta tcagtatccc agtctctgta tgcaaaactc
    11761 caaaatacag tcttgtatcc taaattttta gtcattgcca agcttttttc tgagtatttt
    11821 cccataggag gtctaaaaaa tttaaccata tctttcccag ttacatcttt atataacttt
    11881 tcaacatcat ataattcgtc attaaagttt tttagattgc ttgtttttgt tggcatagat
    11941 ggatgggttt tactatgatt tccaactata tgtccctcag aaaccattct ttttactaaa
    12001 tctttatttt cttttatata tggagttgta acaaaaaaaa ctgctttaac ctggttttgt
    12061 tttagagtgt cgagtatctt tgtagtgtat ccattttcat aaccttcatc aaatgtaaga
    12121 tatagtgttt tttgaccatc ttttgtaggt ccattgtata gggcatcata gtcagaaaat
    12181 ttaaattcaa tgtccgtatt tacttctggt gttttatgct tattgtttgg tatataaaac
    12241 caatcaagag tagtgttatc taaagaagat acatctaatt tattatcatc attattagta
    12301 gtggcttttg atggtttatc tgttccgtct gaaactatta cattggaagt atctttacca
    12361 ctatctattt tttccttatc ttcttgtaac tgtgtttcct tattttgatt ttcgttttga
    12421 ttgttttgtg aatttgaaca tccaacaata aaaaaaatag aaatagaaag tagtgttaaa
    12481 taatgcaata cttttctaaa cattttgaag ctcctttcta aattaataat aattgcatat
    12541 aataataatt atatgattaa ttacaattgc atatagtatt attataattg gaaattacta
    12601 ttaataatag ttatatcttt aaaaataaag tcacaatagt atgacaaagg gtgaataatc
    12661 aataaattta aaaaaaaatg aaaaaaatag ttgaccatat tatgagaaga tgttattata
    12721 ataaatgtgc tcaacaagaa gtaacaaaat aacaagcaca aagaagttaa ttgaat
//
LOCUS       DAELNY010000110        12664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.110, whole genome shotgun sequence.
ACCESSION   DAELNY010000110 DAELNY010000000
VERSION     DAELNY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.110"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            111..761
                     /locus_tag="KPE55_002505"
     CDS             111..761
                     /locus_tag="KPE55_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG0493560.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(901..2277)
                     /locus_tag="KPE55_002506"
     CDS             complement(901..2277)
                     /locus_tag="KPE55_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0493561.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2293..4206)
                     /locus_tag="KPE55_002507"
     CDS             complement(2293..4206)
                     /locus_tag="KPE55_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0493562.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4342..4974
                     /locus_tag="KPE55_002508"
     CDS             4342..4974
                     /locus_tag="KPE55_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG0493563.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5113..5652
                     /locus_tag="KPE55_002509"
     CDS             5113..5652
                     /locus_tag="KPE55_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0493564.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5828..5998)
                     /locus_tag="KPE55_002510"
     CDS             complement(5828..5998)
                     /locus_tag="KPE55_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0493565.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6283..7350
                     /locus_tag="KPE55_002511"
     CDS             6283..7350
                     /locus_tag="KPE55_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG0493566.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7520..9421
                     /gene="cooS"
                     /locus_tag="KPE55_002512"
     CDS             7520..9421
                     /gene="cooS"
                     /locus_tag="KPE55_002512"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0493567.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9414..9887
                     /locus_tag="KPE55_002513"
     CDS             9414..9887
                     /locus_tag="KPE55_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0493568.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9862..11088
                     /locus_tag="KPE55_002514"
     CDS             9862..11088
                     /locus_tag="KPE55_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0493569.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(11227..12414)
                     /locus_tag="KPE55_002515"
     CDS             complement(11227..12414)
                     /locus_tag="KPE55_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0493570.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
BASE COUNT         4785 a         1591 c         2012 g         4276 t
ORIGIN      
        1 aatttgtcaa gtttaattta gttgtaattt tatcttttat taaaaataat ataaattaaa
       61 acatataaat aaaatatttt atacaatata ttgtaaagga ggtaactttg atgaaagcag
      121 aaaaaacaag tatatatcca attattaaaa ataatttaaa caaaatatca attgttgaat
      181 ataaaaaggg tcaaagattt gtagcatcag ataaagactt acaagaagtg ttctttataa
      241 tagagggagt tgcattagtg gagtgtacta ctagaggagg aaataaattt cttgttgaca
      301 ttgtacctga aaatgaattt ataggcaaaa ttagttacat atatgaacat aatttgaaat
      361 gtgatatatt tgcaaagact aatataaaat tatttcgatt tgaaaaaaat gtatttgaag
      421 agttttatct caaaccagat tttattgcac tatttcatag aaaatgtaca agaagaatat
      481 atgaattata taaaaccaga atggtaagag aactattttc ttgcacagaa gttattgcct
      541 attgtatatc aaataaacaa gaaaataatg tctgcaatat aaaagctatt cgtaatttca
      601 gtgaaatcta ttcaattagt agaaaaagta gataccattc tctgctaaag ttaactgaaa
      661 aggagatagt aaaaaaaatg ggtaatacat atgaaatttt aaactatgat gaattacata
      721 aattggcatt tgaagtaaaa gaatttttgg aagatgagta aaaattgtta cttaatcaaa
      781 ataaagtata ataatttctg taaaaaatag aaatagtctc actgaaaatc aaaaatactt
      841 tagttatttt caatgagact gtttagaaat attgtatact tataatgtat agtataatta
      901 ttaatcaatt tcctttacta tattaatagc ccatttttta gataatgctc taggaacacc
      961 aaatataaac ttaaccactt cttcactata agtgagcaaa acgagtacaa atataggtgc
     1021 acctttaaat gcagctataa aagttagagg aatagaagct aaccacatac atcctaattc
     1081 taaaaataga gcatatttag tatctcctcc acttcttaaa ataccaatta taaccaatgt
     1141 attaaaagat ttaagagcca ttagtattcc cattataaag aatatcttag ttatatcaga
     1201 agctaagcta tcagaaacac taaacatttt taataataat ggtatattta aaattaaaat
     1261 aattccaagt attaaaccag ctgaaaatac aagtatagaa aactttttag catattcaat
     1321 ggctctttta atatggtcag ctccaagttc atttccaagc ataatagatg caccagatgc
     1381 aatacataca gctgtcatta tgaagaaatt acctgtactg gttgctatct ggctagctgc
     1441 tatagcagaa gtaccagctc ttgcatatgc aacagaatac aggacagttc caattgccca
     1501 tacagagtca tttacaaata taggaaagct ttttgatgaa aaagatttta taaaaatttt
     1561 atcaatagct tttagatttt ttagaccaaa ctttaatata tagtcttttt tataaaggta
     1621 aacataagta atcattaaaa ttaattcaca tattctagca ataacagtag ctagagcagc
     1681 accttttaca cccaatgcag gaagaccgaa gttaccaaat ataaatccat aatttaaaat
     1741 aacatttgtt acaagtgcaa atgcactaca aatcatacct aacttaggat ttctaacacc
     1801 tcttgaacca gtactaaata cagtacttac agcaattaat ggataactaa atactgcaat
     1861 actaaagtaa tctatacaaa gttttaccac ctcagaatca tacgaaaata tgtgtataat
     1921 tggctttggt gttagtaaag caggaataac aaatacaaaa cttaaaacaa ttgccaatac
     1981 acaagtaaga cctgtgactt ttcttatatt actcacatcc ttctttccat aaaactgtga
     2041 aataaaaact ccagcacctc ctgtgatacc agataaggac atattaaaaa gaaagaaaaa
     2101 ttggtttgca actccaactg aagctacaga tgtttccccc aaactactta tcataattgt
     2161 atctattaca tttacagaag tagaaattaa gttctgtatg ataataggta tacataaaga
     2221 tagaagtatt ttataaaatc ttttattttc gtcttttaaa gaaaatagtt cttgcaaata
     2281 atcctctcct ttttacataa attcttccca ctcatcatat agagaggcaa ggttattttc
     2341 taaattaatt ttttcttggc taacttcttt agctttatct ggattagagt atacttcttc
     2401 ttgacataaa agtatatcta atttttcaat ttcaatctct aacttttcaa tttctttttc
     2461 tatattttgt ctttttattc tatttttctt ttctatttct ctttgttctc tctcttttct
     2521 tttttcatct tttatttgag ttctagtttt ttcttctttt tcttctataa tactcatctc
     2581 ttggatttgc ctttttttat ctatataata atcataattt cccagatatt cagtaattcc
     2641 attttcatct aaaactaaaa ttttatcaac aacagtattt agaaagtatc tatcatgtga
     2701 tattgtaaat atagtcccat cataattagt taaggcctct tcaaggactt ccttagaatc
     2761 aatatctagg tgattagtag gctcatctaa taacaataag tttgcatttg atagtataag
     2821 tttaagtatg gcaactctag ctctttctcc accactcaat gtagaaatct tcttaaaaac
     2881 ttcttcatct acaaataaaa atgcccctaa catatttctc aaagtagttt gagtaagatg
     2941 agtattattt tcccaaatct catcaattat tgtgttatct aaattaagag ttttttgttc
     3001 ctgatggaag taggaaacat gtacattagt tccaaattta atattgccac taagaggttg
     3061 tagttcatta gttattattt taaataatgt agatttgcca acaccatttg cacctataag
     3121 tgcaactttt tcccctctat atatatctaa atccaagtct ttaaaaagta ttctttctcc
     3181 ataacccata gatacatctc taatttgaag aacatcatta ccactctgga cagaaggcgt
     3241 aaattgtata cgagcttttt tcctataagc ttctggttta tctaaaacat ctactttgtc
     3301 taatgctttt tctttacttc ttgcacgctt taggtgtttt tctctaccat aagcttttag
     3361 tctttcaatt gattcttctt gcttttttat ttctttttgt tggtcttcaa atttcttaag
     3421 ctctagttct ttttctattt tagataactc aacaaattta gagtagttac cattatatac
     3481 tttaagcttt ttattgtgaa cttcaaaaat tctatttacg gattggtcta agaaatatct
     3541 atcatgagat attaacatga cagtcccttt atattgtttt aaaaatactt ctaaccattc
     3601 tattgcttca gagtctaagt ggttagtagg ttcatcaagt aacaatagag ttggtttctt
     3661 aagaagtaat tttcctaaaa gaactctagt tttttcacca ccagaaagta tgcttattgg
     3721 cttatccata tcattgtcac taaatcctaa tccctttaga actcctttgg cttcagattt
     3781 atatccatat ccatttaaat ctgaaaataa ttctagttta tgtgaatatt catccatgat
     3841 tttttctata attggagagt ttgtttttgt actttcttca gatattttaa cctccaattc
     3901 tcttaaatag gattccattt ctatcaaatt tttaaaaact tctagtactt cttccaaaat
     3961 agtattatcc gaataaaaat tggtattttg ttctaaatat ccgatttcac aatctttaga
     4021 agtgtaaata tctccactat catatccata aattcctgaa attattttaa ataaagtagt
     4081 ttttccagta ccattaacac cgattatacc aattttatcg ccttcattta ctgtaaaact
     4141 aatattttct aatatagaat ctataccaaa acttttattt aagttattac atgacaaaac
     4201 aatcataaat ttctacctcc ttgaactatc taaaattata atgttttgat ataatataat
     4261 caaggttata tgtattagtt ttgaaaaaga aatattttat tgtgaatcaa acaaaaaaga
     4321 ggacactttt aggaggtgaa gatgttggga aataaaaata tatcaatggc agttataaga
     4381 aggctcccaa aatatcatag atatcttgga gacttattag atagggatat acaaagaata
     4441 tcttctaaag aattgagtga tataataggg tttaccgctt ctcaaataag acaagattta
     4501 aacaactttg gtggatttgg acaacaagga tatggttata atgtagaagc tcttcatact
     4561 gagataggta aaattcttgg gttggatcga ccatacaacg cagttcttgt aggagcaggt
     4621 aacttaggac aagctatagc caattatgca ggatttagaa aagctggatt cgagataaaa
     4681 gctttatttg atgcaaatcc tagaatgata ggtttaaaga taagagagtt tgaagtatta
     4741 gattcagata ctttagaaga ctttataaaa aacaataata tagatattgc tgtattatgt
     4801 atacctaaaa atggagcaca agaagttatt aatagagttg taaaagctgg aatcaaaggt
     4861 gtatggaatt ttgcaccttt agatttagaa gttccgaaag gtgttatagt tgaaaatgta
     4921 aacttaacag aaagtttatt taccttatcg tatttaatga aagaaggaaa gtagacaact
     4981 taaacatatt aataatgagc agttgtaaag agtattgaga aagtcagaag atttaatatt
     5041 aaatcctctg gctttttgaa atattaggat aaaaatttaa ataatgtatg gaaataaaca
     5101 atattaagga gagtggtttt tgtggaggaa ttatggaaaa aggcaattga atttcatggt
     5161 catgaatgtc cagggttagc tataggattt aaagcagcac ttgcagcaaa aaaatattta
     5221 ggatgtaaac agtcaggaga tgaggaaatt gtatgtattt ctgaaaatga tgcttgtgga
     5281 atagatggta tacaagtagt gctaagttgc acaatgggta agggaaattt actacttagg
     5341 atgactggaa aatcagtata tcatttcttt aatagagaaa atggaaaatc tataaggata
     5401 tatgtaaagt caaaaccatt tgagatggat agagaaggat acaaaagata tttattagaa
     5461 agtgattttg aagaaatatt tgagattaca gatactaagt taagacttcc tgaaaaagca
     5521 agaatattta aatcccttaa atgtgaagtg tgtggtgaat ttgcatctga gcaatatata
     5581 aggattgaag aaggaaagaa agtatgtctt gattgttttg ataaatatga aagattttat
     5641 gaagagtatt aaaattataa attagattaa agttaaaagt attagatatg tgctaacagt
     5701 attttaagat gaattaggta gcaacataat aaagtatata aataatatta attgaatcat
     5761 acataattaa aaagaggctg attagtattt ataaaataaa atcagtctct ttatgaataa
     5821 cgaattatta ttctggttga ggtgcatcta caggacaagc tcctgcacaa gaaccacagt
     5881 ctatacaaga accagcatct ataacatatg cgtcgtctcc agctgatata cagctaacag
     5941 ggcattcagc ctcacaagca ccacagctta tacaagcgtc agtaatttta tatgccattt
     6001 tgaaatcctc ccttttatat gtattgtaat tcaataacat tataacaaat attattgaat
     6061 ataacaatta aattaattct acaacaaaaa ctttattatg atattcttgt tttaaagttt
     6121 tacaataaag tttaatctgc ttatattttt atttatttta aagaagttta ttttaaaaat
     6181 aaaattaaat ttaaaaagtt atgtattgat tatatttaaa aatgttatac tctaaaaaag
     6241 tatattttat ttaaaaattt attaatacaa aggagattta ctatgaaaaa taaattagtt
     6301 tacatatgta ttatgagtct attgctattt gtagtaggat gttctaataa tccaaataag
     6361 gaaagtacca ttaaaaaaga ctctaagaat aaccaaagcc aagttaaaga agaaaaaaga
     6421 gaacctgtga aaaaagatat aactataagt ttcgctggtg atgtaactat ggggaactat
     6481 aaaggttcat catattatgg cagttttgac catgaattta aaagacaagg agaagattac
     6541 aattattttc ttaaaaatgt aaaacctgta tttgaaaaag atgacttaac agtggttaat
     6601 ttagaaggtc cacttactac agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga
     6661 aagcctggat atgtaaatat tttaaaatct ggaagtgtag aggcagtaag tattgcaaat
     6721 aatcattcag aagattattt tgaagaagga atgaaagata cacagtttat attggacgag
     6781 aataaaataa attattttgg attagggaaa gatgctgttg tagatgtcaa aggaattaaa
     6841 gtgggtctat tagggtataa tggattatca actgaatata atgaaaaaaa tttaaaacaa
     6901 atggaagatg atattaagtc cttaaaaaag aaatcggatg ttgttgtagt gtactttcat
     6961 tgggggatag aattagatta ttatccagat aaaaaacaaa aagattttgc tcattatgct
     7021 atagacaagg gagcagattt agttatgggg tctcacccac atgtgattca aggaatagaa
     7081 aagtataaaa ataagtatat agcttatagt ctagggaatt tttgctttgg aggaaataag
     7141 aatccttctg atacagatag ttatatatat caacaaacct ttactttttc agataataaa
     7201 ttaacttcaa ttaaagagcc aaatataata ccaacttcaa taacatctag taactccaga
     7261 aataattatc aaccaaaaat attggatgga agtgagaagg atagagtttt aaataaactt
     7321 gaaaaaataa gcaaagattt aaataaataa aatgtaaaaa aaaataaata tatatattta
     7381 ttttaataat ttatattttg tttagggtat ggtaaatcgt ggtattaaat aagtagattt
     7441 taatagttaa attatagtca attataataa agctatttaa tctacttttt taatataaaa
     7501 atataagggg gaatttgtaa tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa
     7561 gaaattagaa agttttgtag cttcaaagga tgttgaaaca gcatttcata gaacagaaga
     7621 tcagaaagtt aagtgtggtt ttggacttca gggggtttgt tgtagactct gttcaaatgg
     7681 accatgtaga gtaactccta aatcaccaag aggtatatgt ggagcagatg cagacactat
     7741 agtagcaaga aactttttaa gagcagtagc atcaggagca gcttgttatt tacatgtggt
     7801 agaaaataca gctaagaatt taaaaaatgt tggaataact aaaggtgttg ttaaaggaga
     7861 aaaaacatta aatcaattag cagagatgtt tggaatagaa agtgatgaaa agtatgataa
     7921 atgtataaag gttgcagaca aagttatcaa cgatttgtat aaatcaagag atgataaaat
     7981 ggaattagta gaaaaaatag cttatgcacc tagagtgaaa aagtggaaag agttaggtat
     8041 aatgcctggt ggtgcaaagt cagaagtttt tgatgcaata gtaaaatcat caacaaattt
     8101 aaacagtgac ccagtagaca tgctagttaa ttgtttgaac ttaggtatat caacaggatt
     8161 atatggtctt acactaacta atcttttaaa tgatgttatg ttgggagaac cagttataag
     8221 aatggctcct gtaggattta atgtaataga cccagactac ataaatataa tgataacagg
     8281 tcatcaacat tcaacatttg caaatttcca agatagatta aaagatgaag atgttataaa
     8341 attagctcaa tctgttggcg caaaaggatt taagttagta ggatgtacat gtgttggtca
     8401 agatttacaa ttaagaggag aacattatca agaagtattt gctggacatg caggtaacaa
     8461 ttttacaagt gaggctgttt tatcaactgg agcaatagat atagttcttt cagaatttaa
     8521 ctgtactata ccagggcttg aaccaatagc agataaatat aaagtaaaaa tggtttgttt
     8581 agatgatgtt gctaaaaaag cgaatgcaga atgcataggt ttagacagaa gtaaattaga
     8641 tgaacttagt aacactttaa tagaaaaagc attggagtct tataaggaga gaagaggaag
     8701 tattgaaata gatataccaa aagaccatgg atttgaacaa tctttgactg gtgtaagtga
     8761 aaagaacttg aaagcgtttt taggagattc atggaaacct cttattaatt taattgcaga
     8821 aggaaaaatt aaaggtgttg cagctgttgt aggatgttca aatatgacag caggaggaca
     8881 tgatgtaaat actgttgaac ttactaaaga attgattaaa aaagatataa tagtactatc
     8941 tgcaggatgt tctactggag gattagaaaa tgttggtctt atgtcacctg gagcagaaga
     9001 attggcagga gaaaacttaa aagaagtatg taagacttta ggaataccac cagtattaaa
     9061 ctttggacca tgtctagcta taggaagact agaaatagta gcaacagaat tagcagctga
     9121 acttggaatt gacctaccac aattaccatt agtgctttca gctccacaat ggttggaaga
     9181 acaagcatta gctgatggag cttttggtct tgcccttggc ttacctcttc atttagcttt
     9241 acctccattt ataacaggag gaaaattggt aacagaagta ttaacagaaa aattaaaaga
     9301 cttaacagga ggacatgtta tagtgaatcc agaccctaaa tcttcagcta atcaattaga
     9361 agaaataata atagatagaa gaaaaaattt agggttaaag gatgtggaat gcaatgcata
     9421 gaatttttat aaataaagat ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc
     9481 taaaacacaa taaagattat gacatgtaca ctcttgattt agaaaatata gataatgata
     9541 gtagagggca tatagaatta gatagtagac ataataatcc agttccaatt ctttgtagac
     9601 actgtgatga acctgaatgt gtattagcat gtatgagtgg tgcaatgcat aaagatagtg
     9661 aaagtggaat cgtttcatat gatgaagaga aatgtggttc atgttatatg tgtgtaatgt
     9721 cttgtccata tggattatta aagccagatg atagaagcaa gcagaacata cttaaatgtg
     9781 atttatgtaa agatgaggag tatcctagat gtgtagctaa ttgcccaagt ggagcaattg
     9841 aattacagaa ggaggaaaat gatgaattat gtagtgttag gggctagtgc agctggtata
     9901 aatgcagtaa aaacattaag agaattagat aaagattcaa atatagtagt aatttctaaa
     9961 gatgaaaacg tatattcaag atgtatgttg catcacgtta tatctgaaca cagaacttta
    10021 aaacaaataa attttgttga tgaagatttt atggagcaaa ataatgtaaa atggattgca
    10081 ggtaagactg ttaaaggtat agacataaat aaaaaggtag ttcagacaga agacattact
    10141 gtaaattatg ataaactttt aatagctaca ggtgcatctt ctgctattcc tcctataaaa
    10201 aatttaagag atggaaattt tgtatattct gtgagaaaca tagatgatat ttataaaata
    10261 aaagaaaaag cacaaaattc taaaaatgta gttataatag gtgctggttt agttggtata
    10321 gatgcattag tgggattatt taaatatgag cagttaaata tttctgtagc ttttatggaa
    10381 aagtacattt tagatagaca attggatgaa tatacagcat cagtgtatga aaataagttc
    10441 aaagaaaaag gtgtaaaatt ttacccaagt gcatcaatac aagaaattgt attggataac
    10501 agtaaaaatg ttacaggtgt tgcattttca aatggtgaag ttttagatgc agacatggta
    10561 attgtagcta caggggttaa accaaatgct gactttttag atggaacagg aatagaatat
    10621 gatagaggta taataataga tgatatgtgc caaacaacac aaaaagacat atatgcagca
    10681 ggagatgttg taggtaaaaa tgctatttgg cctctggcag taaagcaagg aattgtagct
    10741 gcctataata tggttggaaa agataagaag atagaagatg aatttgcttt taaaaatagt
    10801 atgaatttca tggatatacc aactatttct ataggaatga atacacctgt agatgatagc
    10861 tataaagtat taaccagaca tggaattaat gattataaaa agtttgtttt taaagataat
    10921 gtaatctatg gagcagttat acaaggagat atttcatatg taggtgtatt aacttattta
    10981 ataaaaaata aagttgaaat atatgattta gaaaatagga tatttgatat atgttatgca
    11041 gactttttta atataaaaga aaatggagaa ttttgttata gtgtatagta tatacgtgga
    11101 gtaaggttta taaatacagt aaatcaataa atagagttat tctaaaataa gaaaaatgac
    11161 aaatcttatt tagaaatagc tctatttttt gtaattaaat ttaaattata ttcaatttgt
    11221 aaaatattat tttgaacaat tatacatata ctctctagtt agaggtaaat cattttttat
    11281 acctttagtg aaaagtattt gatgtatatc tataatacca gtgttgaaag cagcagcaca
    11341 agaacaaagg tataaatccc acattcttat aaacttatca tcaaacattt ctttaatctt
    11401 tggaagattc tcagagaaat ttttttgcca gtatagtaag gtttttacat aatgtaatct
    11461 taaactttct acgtctatag tatgatattt gtaatcacta caaatatgta tcatttctct
    11521 taggctagga ataactcctc ctgggaaaat atattttttt atccaagggt ctccataaga
    11581 ttcctgcaat ccacttatat agtgaagtaa aaacaatcca ccatctttta aaacagcatt
    11641 tacgttttta atgaaaagtt catagttttc tctaccaaca tgttcaagca tcccaacgct
    11701 tacaactcta tcaaacataa ggtctgattt ttttaattct ctatagtcca taagtcttac
    11761 ctcaagatag tcttgaaggt tctcttcttt aattcttgcg ttaaattcct taaattgctc
    11821 ttcgctaagt gtaattccaa cgccgtgaat tttatatttt tttgcagctt ctattagtaa
    11881 aaatccccaa ccacaaccaa tatcgagaag gctcatacct tcctctaaat ttagtttagt
    11941 taatatgtgg tgaactttat tgacttgagc atcataaaga gtatcattat catttctaaa
    12001 gtatgcacaa gaataactaa gtgtcttatc taaccataag ctataaaaat cgttgccaat
    12061 gtcatagtgg gcatgtattt ctttcttttg atgtctcatt gaatttgatg agtgcaatag
    12121 atgttttaca gctgaattat caatattgaa tttgtctatt tgttttaaaa gtagatttaa
    12181 agttaagaat aaatctccct caacttcaat gtctctattt atgtaggctt ctccaagtgc
    12241 taaagatgta ctttttaata gttcagactt gctaatatca ttatttacta taactttgaa
    12301 ctgaggggag ccctctccaa ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg
    12361 tgtttcacca aatttctcta aatagtcttt taaaaaattt tttgtaatat tcatattacc
    12421 agctccttct aatgtaaatc cagtaagttt ccctgcttaa aatatattat agtccttttt
    12481 ttaaaaaagt aaaatttttt caataaattt tttatttaaa ggaaatttat tttttgtaaa
    12541 gaaatttcat gttgtagtat attttttgct tataaatttt ggtactggat ataatattaa
    12601 actgttaatg atataataaa tttaatctat aaagtaaata aaattttgta ataaatagtt
    12661 aaag
//
LOCUS       DAELNY010000111        12624 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.111, whole genome shotgun sequence.
ACCESSION   DAELNY010000111 DAELNY010000000
VERSION     DAELNY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.111"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(246..1106)
                     /locus_tag="KPE55_002516"
     CDS             complement(246..1106)
                     /locus_tag="KPE55_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0493571.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1255..2145
                     /locus_tag="KPE55_002517"
     CDS             1255..2145
                     /locus_tag="KPE55_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493572.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            2363..4117
                     /locus_tag="KPE55_002518"
     CDS             2363..4117
                     /locus_tag="KPE55_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG0493573.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            4501..5487
                     /locus_tag="KPE55_002519"
     CDS             4501..5487
                     /locus_tag="KPE55_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0493574.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            5656..5804
                     /locus_tag="KPE55_002520"
                     /pseudo
     CDS             5656..5804
                     /locus_tag="KPE55_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            5823..7304
                     /locus_tag="KPE55_002521"
     CDS             5823..7304
                     /locus_tag="KPE55_002521"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG0493575.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            7330..8301
                     /locus_tag="KPE55_002522"
     CDS             7330..8301
                     /locus_tag="KPE55_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG0493576.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(8557..9159)
                     /locus_tag="KPE55_002523"
     CDS             complement(8557..9159)
                     /locus_tag="KPE55_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0493577.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            9440..10651
                     /locus_tag="KPE55_002524"
     CDS             9440..10651
                     /locus_tag="KPE55_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG0493578.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            10719..10916
                     /locus_tag="KPE55_002525"
     CDS             10719..10916
                     /locus_tag="KPE55_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG0493579.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            10950..11921
                     /locus_tag="KPE55_002526"
     CDS             10950..11921
                     /locus_tag="KPE55_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0493580.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            12108..12485
                     /locus_tag="KPE55_002527"
     CDS             12108..12485
                     /locus_tag="KPE55_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0493581.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         5151 a         1316 c         2005 g         4152 t
ORIGIN      
        1 tgttataaag taaagtaagt aataaagtta gactcaataa ttctaaaatt tctaagcata
       61 ctatctaaat gagaacggat ttggaaatac ttgtataaaa caataattga tacatttaat
      121 ttttatctta aaaacaacaa ttataatata taaatagcaa gtataatata taaatagcaa
      181 gtataatata acatataaaa agttgaattt gtatatgtta attatacttg ctatttattt
      241 gttagttatt ctattttttt aaatccatta cctaaagctt catgtatatc tgtaattata
      301 ataaaagcat tagggtcgac attttttact aattttttca attcgacaac ttctttcttt
      361 tcaactacta ctaaaagtac atttttattt tctccagtat atgcaccttc tccttttaga
      421 atagttgcac ctctatctaa atcttccatt atttttttac ttatatcttt tgaaaggtca
      481 gatataatag tgaatgattt ggaataatta aatccttcaa taagcaagtc actaactttt
      541 actaaaatgt ataaagccac agttgaataa agtgcaattt caacctgtct atttactata
      601 cctgataaaa caactacaac gcagtctaca atacccatta aaacaggtat actaaatgta
      661 ggaaaatatt tatttagtag gagtgccatt aaatcagttc cacctgtaga gccatttatt
      721 ctaaaaataa gtccttgacc aactcccatt aaaatagaac cagatataat tgcaagatac
      781 atatcatttg ttatatctaa tgtagccata tttgcagtaa gcttaagtgc tatggtaaga
      841 aaaattattc ctaaaaccgt tttaaaacaa tctttttttg aaagtatttt gtaagcaaat
      901 atgaataatg gaacatttaa aagtaaattg acaatccaaa gttctacacc gaataatgaa
      961 tttaaaacaa ctccaagtcc tgttatacca ccagcagcga ttgcatgtgg attaaaaaat
     1021 aagttaagag aaatggccat taatatacag cctacaatta atattaaaat ttctgaaaaa
     1081 gttattctat cttttttcaa taacatcact ccttatatat agattataat atatattata
     1141 tagtttttcc aatttttaat aaaagaaata aaaatatatt ataaaaacaa tttgattgga
     1201 attttcagaa aattaatgct ataattaaaa gtaaatttaa agggaggtta taaaatggct
     1261 aaattaactc tagaacatat aaaaggaaat acatattata ttccactccc tacaatagta
     1321 ggtgtatacg tagatggtaa ggatgcaatt ttaattgaca gtggaaataa taaggataca
     1381 gctagacaag ttttaagatt attagaagaa cataatttaa atcctaaatt aataataaac
     1441 actcattcaa atgcagacca tataggagga aatgcatatt tgaagaatca aacaaaatgc
     1501 aagattgcta cgactaaaat tgaaggatat tttacagaaa atccaatatt agaatcagca
     1561 tttttatatg gtggctatcc aagtaaagca ctgaagaata aatttttatt agcaaaggaa
     1621 agtgaagttg attatattat accttcaaat ggcaaaattg tagatacaga acttgaagcg
     1681 atttctcttc caggtcatta ttttgaaatg attggtgtta gaactccaga taatgttttg
     1741 tttgttgcag atagtcttac accagagaat ataattacta aatatcattt tttcttccta
     1801 ctggatatag attcacagtt taagacacta gaaaaattga gaatgttaga ggctgatttc
     1861 tttgttccaa gtcactctgt caaaactaca gatataaaaa gtttaattaa tattaataag
     1921 aagaagatgg aagaaataat agataatatt aaaaaggttt gttatgagcc agttatgatt
     1981 gatgaagtta ttgaaaaaat gtgtgatttg tacaatgtca agcttgatgc taatcaatat
     2041 gtgttggttg gaagtacaat acgttcttat ataacttatt tatatgagaa taatatggtt
     2101 gaatatatat ttgatggtgg taagatgatg ataaaagctg cgtaaatagt aataattttc
     2161 aaattatatg atgaaattgt tttagtatga ctgttatttg aaaagtaata taaaaaaatt
     2221 tatataaata agctaagatg attgatataa agttactgat aattttaagg taatatatac
     2281 gttactataa tattatgtta ttatttgtaa ataaggggat aatttataag caaattaaag
     2341 accttaattt acaggaggaa tagtgtatat tatgaataaa aaattcaaaa aattcagtaa
     2401 aaaactaagt atattaattt tagttttgac tatgtttctt tttaataatt taactgcaaa
     2461 tgcacagatt aaagaagatt caataatagg aattgataga tatgaaacag caggacttat
     2521 agcggataga caaacttatg atactgtaat attagttaat ggagataaaa atttatctga
     2581 tgggctttca tcaagtggat tagctggagc tattaacgca ccaatacttt tgactaaaaa
     2641 aagtgaaata ccaaaagcta ctttaaatag actagataat aagagtttaa atgttgtaaa
     2701 aaaagtatat ataattggtg gttataatac tattgaaaat tcagtagaaa aagatataaa
     2761 aagaaaaggt attcaagtgg aaagaataaa tggtaacaat agaattgaaa ctagttataa
     2821 tgtagctaaa aaaataaatg aagtaagtag tgtaaaagag gtaatgctta caaatggatt
     2881 tgtaggagag gctgatgcta tgagtatagc acctgtagtt gcaaaaaata aaggagctat
     2941 aatacttaca gatgggaaat cagttccatt taagactgaa ggtttaaatg tttacgctat
     3001 aggtggcaaa tctgcgataa atgaggattt agtaaatgaa actaaagcaa ttagaatcgg
     3061 aggaaatgat agatttgaga ccaataaaaa ggtaatggaa aagttttata atggagctac
     3121 agatttttat ataacaaatg gatatcaatt agtagatgca ctaacattat ctcctttagc
     3181 caaagaaaaa ccaatcgttt tagttgctga tggaagtaat aaaggtattt taaaaggagc
     3241 taaaagtata acaaaagttg gaggtattga tgccaatata tataaacagt gtttagatat
     3301 gattaaatat aataatatgg atatgactcc aaatatttta aaacacttaa cttcagttga
     3361 aggaaatgaa gtttctgaaa tacttataga atcagataat ttaggtgtgg ttgttgaaaa
     3421 gacgaatacc aataaatttg agtttgatta cgtaagtgta actaatgaaa aaaattgtac
     3481 tttttctgta tataaagaaa attcttctaa taatataaaa aatggaaagc tagttgtaag
     3541 tgctaagaaa aaaatagata agaaagatag tgatttgcaa gatatgaatg gcgacaatat
     3601 gataaatgca aataaagata ggatggtaaa tgtgataaaa attggagttc ccgataaaat
     3661 gtattctaat tttaaagtag atgttgaaag tggttcagta gaactttata atattaaagg
     3721 aggagctact gttgatgtca atgacggaat tgctaacatt gttgataaca atgtaacaaa
     3781 tcctttcaat ataaatacta atagtgggat ttcaggtgta acagctgaaa ctatatcatc
     3841 tgaaataaaa tttagagcaa acaatggaat tgtaaatgta acagctacaa atataaatgg
     3901 caatataagt ttatttggaa gcgataataa agatactttt gatggaatat ttaaattaaa
     3961 tctgaaaaaa gaaccatcaa atttacactt gaagttaatt ggaaatggga taaataaatt
     4021 gccagaaggt tggtctaaag attatatttt aggaaatgga caaccaatta ttgaagttaa
     4081 aaataatggc ataaataata tatcaattgg agaataaaaa catatgaaaa taaagtttat
     4141 atagttttaa aaattataga attttaaaat atttatatat attctttaaa taaaaaatta
     4201 ttttttaagt gataattaag tgtttatcga taaggctatg gttgtaaaaa tcatagcctt
     4261 atttttatat tataaataaa ttagagtatt tatcaattca tagtagataa aattgaaatt
     4321 aaatataaca acaaaaaatg aaaattacaa ttaatttaat gttaaagact caattatttg
     4381 gatgtggaat ggatagtaca agtagtatat aaaaagtatt gactggacaa ataactatat
     4441 tttattttaa atatataata taatgtaata aaatatatca aatgtagaag gtgataacct
     4501 atgaaaaaaa taacaataaa tgatattgct aacttggcag gagtatctaa aagtactgta
     4561 tcaagatatc taaacaataa agatatcagt gattctacta aagagaaaat aaagacaata
     4621 atagatgaat atggatatga gccgaatgca tttgctcaaa gtttgagagc taaaaaaaca
     4681 tattttattg gtattataac accgtgttta gattcttttg taaaatctaa aattatgatg
     4741 gcaatagatg aagaactcaa aaaattaaaa tatacatcac taattattaa tactaataga
     4801 aaaataaggt cagaaataga tagtatatca aagttagcta gtctaaaagt tgatggaata
     4861 atactaattg gtaccgaaat aactaaagaa cataagaatg aaattgaaaa gttagatata
     4921 ccgatagttg tagttggaca aaaagttgat ggtatcaata gcatagtgaa tgatgattat
     4981 ggagcaggct ataaaatggg tcaatatatt gctaataaag gttacaaaaa cattgtatat
     5041 ttaggtgttg atgaaagtga tatatcagtt ggtttaaata gaaaaaatgg agtacttaat
     5101 gggttaaaag ataaagggta tgatgcaaaa gtattttata ctgactttga ccaagaaatg
     5161 tctatacaaa gaagtggaga aatgctagaa agtgaaaatc cagatataat aatatgtgca
     5221 actgataata tagcaatagc aacaatgaaa gaaatcaata agagaggtaa aagtatacca
     5281 caagatatat ctgtagctgg atttggggga tatgatgtat tgtctattat aagccctaaa
     5341 cttacaacaa taaagtttga aaataaaaat gcaggtaaag tagcagcaaa tacgatagta
     5401 aatttaatac aagaaagaaa agaaccacta ttaaaggaga ttaaatttga attaatagaa
     5461 ggtgaaagta caataaataa aaattaagat acacattaca tatattagta atgtgtattt
     5521 ttttactaaa aaaactataa ttctaaaaat taggaaaact tttttgaaag aaatattgca
     5581 tctgaaataa atttatgcta atctttaatt aaaccggttt cgaaaccggt ttaattaaac
     5641 cgatagaata gcaaaatgag tgcaaaaata actgattgta acaaagtatc aaagtaatta
     5701 ttggagtaca taggaataaa caaaatataa aagatgtagc acattgtgta ataagactaa
     5761 gaattgtttt agatagcaat aataaagcaa atataaaatt ataaaagaaa aggaataaaa
     5821 atatgtacaa agagcctaaa tatagaacca tattagaatc gacaaaagaa gaattaaata
     5881 aattaagaaa tatatcttta aatgataaat ataaaccact atttcatata catccacaac
     5941 atggactact taatgaccca aatggattag catattataa tggtaagtac catgtatttt
     6001 atcaatggta tccatatgat gcaactcatg gaatgaagca ttgggcttat gtaagttctg
     6061 atgattttgt aaactggaat agagaagatg tagcattaat accaatcgaa agttatgaat
     6121 ctcatggagc atattcaggt aatgcgatag aggtagatgg aaaattgcat atgtattata
     6181 cagggaatat aaaatatagt gcagaagata gatatgcata tcaaaactta gctataatga
     6241 acaaggatgg taaaataact aaatatgaaa ataatccaat agtaagtgaa ataccaaaag
     6301 gatatacagg acatgtaaga gacccaaaag tatttaaaag aaaggataaa tactttatgt
     6361 tacttggagc acaaacaagt gataaaaaag gagttataat agtatatgaa tctaaaaact
     6421 ctatagattg gaattttaaa ggagaattaa atgtaaaaaa catagatgaa gattttggtt
     6481 acatgtggga atgtccagat tatattaata tagatgaaaa agatatatta atattttcac
     6541 cacaaggagt agaacctaaa ggatttgatt atcaaaatat atataatgta gtatatgcta
     6601 ttggtaatat ggatttagat aatttaacat ttgaaataga tacaatgaaa gaactggaaa
     6661 aaggatttga tttctatgct ccacaaacat ttataaagga ctcacaaata atactatttg
     6721 catgggctgg aatgggagaa gttttatacc ctacagataa aaacaaatgg gcacattgtc
     6781 taactgttcc aagaaaatta aatataaaaa ataataagct attgcaaatg ccagtagatg
     6841 aattaattaa attaagatat gatgaaacaa gtgggcaaaa tactataaag aataatatta
     6901 atattataga aaatgatgaa aatgtatatg agcttaatat taatataaaa aatattgaca
     6961 gcaataaatt tgggctggaa ttattttcat cacaagatga aggtgtaaaa ttagaattta
     7021 acaaattagg aaacattgta actttagaca gaagcaactt taaaaaagtt ttcggtgttg
     7081 aatatggaac aaatagaaaa gaatatataa atattgatga aaatactaat atcaaagtac
     7141 tagcggatag aagtatttta gaaattttta taaatgaagg agaagtagta tttactagta
     7201 gaatatttgc taaagaaaat tctaatcaaa taagggttta tagtgataaa atagtagagt
     7261 atgagtatac taaatttaaa ttaaagcaag gtattgaatt ataaaaacac attataaggg
     7321 gtagaaaaga tgaaaaaagt aattagtata ggagaagctt taatagactt tatacctaat
     7381 caaaatggtg gtaaattaaa aaatgtatca gaatttagaa gagtagcagg aggagctcca
     7441 gcaaatgtta gtgctgttgt agcaaagctt ggaggaaaat cgagttttat atctaagtta
     7501 ggaaaagatg catttggtga ttatataata gatgtattaa atgaagtaaa tgtaaatact
     7561 gattatgtat tgagaacaag taaagcaaat acaggacttg cttttgtatc attaaaagaa
     7621 gatggtaatc gtgatttctc attttataga aatccaagtg cagatatgct tttagaagct
     7681 gatgaagtta aaaaagagtg gtttaataat tgtcatatcc tacatttttg ttcagtagat
     7741 ttaatagata gtccaatgaa attagcacat aaaaaagcaa tagaatacgc acttgaaagt
     7801 aatagtataa taagctttga ccctaatata agattacctt tatgggatag tgagcaatct
     7861 tgtaaaaagg caatatcaga atttttacct tttgctcata ttgttaagat atctgatgaa
     7921 gaattagaat ttataacagg agaaaatgat atagaaaaat cattacataa actctttgtg
     7981 gggaatgttg aactagtatt atatacaaaa ggaaaagatg gagtagatgc atacactaaa
     8041 aaagtaaaag gtatgtgtaa aggggttaaa gtaaatgcaa tagatacaac cggagcagga
     8101 gattcttata taggttcatt tttatacacc ttattgtata aacaaataac attgaatgat
     8161 ataaaagaaa tgaaacaaga aacgttaaat gagtatttag aattttctaa ttattatgca
     8221 gctcgtagta caactattaa aggtgcgata agttcatatg caacaaagga agaaataact
     8281 gaatttataa aaaatttata gtaggaatga gtaataggtt ttgaaggaag taacatgtaa
     8341 agagttttta aggatgttta ctatgaaaat aattaacata taaaaagcaa taaggaggct
     8401 gtctcaaaat agagataagc ctctttatca ttgttgaaat gataaaaaat attttaatta
     8461 ttgttacttt ttagaagaat atatatctta ttttaaataa gcaaattatg taagatttat
     8521 ttatattatc aatactttac ttggagtatt atgtctttag cagttatcta attcaattga
     8581 attagataaa atataataaa tttgttttgc aaattgacga gaatgttctt ctttttcttt
     8641 attcataaaa ttaggtttac ctaaatcacg ttcaacaagc attcctatat attttaactg
     8701 tgaatgctta cagtagcgct tcataccatt ttcccaaata tcagcaccct tttcaggtct
     8761 atatccacat gttgtaataa gtgctactga ttttcctgcc catagagcgg ggcctttctc
     8821 attaccatag tatttattca ttccatacac caatctgtca agtagtgctt tcattggaga
     8881 agtgcagtac caagaataaa ttggagtagc aaaaacaatt aaatcacaca taagaatttt
     8941 cttgaaaatt ttctgaacat catctttaca ataacatcca aaatcaaata agttatcctg
     9001 acacttttta caagcaatac atggctctat tttacaatca tataaccata ttacttcaat
     9061 atttgaatta taattttcta attcctcaat aaaaggtttt aaaatagcag cagtattacc
     9121 ttttttctta ggacttccca taagaatgca tacttccata ttgatacctc cacagtattt
     9181 taaaatagtt tttagtgctt aatatagttt taggatacta tgagaatacg ttaacaacaa
     9241 tacactttgt atatttgtaa taaaattgaa acaaattgtt ttatcaataa aagttggaat
     9301 gtatctataa ttaaaaatat tttaggtatg tttttacata tatgtgtgta gttaactatg
     9361 gtaatttatt gtaaaattat cttataggat tataattgct taatttatga ttatatttaa
     9421 aatcttggag gtaaaaaata tgtactcaaa tgaagactat aaatttatag aagaaaatgc
     9481 accatgttat atatatgatg agactaatat aattgaaaaa tgtaaggaat tgaaaaataa
     9541 tattttaggt tttgattttt tatattctat aaaaacaaat ccatataaca aaattgtaaa
     9601 aagcattgca gagcaaggtt ttggagcaga tgcttcttca actaatgaag ttatgatttc
     9661 atcaaaatgt ggagtttcta aagatgatat ttattattca tcaccaggca aaacagagat
     9721 tgatatagaa actgttttag ataaatgcat aattattgca gatagtatta cagaaataga
     9781 acttataaat aagatatcta aaaagcataa tcagaaagtt aaaataggaa taagaataaa
     9841 tcctgatttt actatggatg atgatagtgc taaatcaagc aaatttggta tagatataga
     9901 agatatgagc agtgttttta agatgttaag tatgtgtgat tatgtatctg tagttgggat
     9961 tcatatacat attaaatctc aggtattgga ttttgaaaag ttaggacatt attatgaaaa
    10021 atgttttttt atagctttaa atttaaataa aagtaagtat attaatattg aatttataaa
    10081 ttttggtagt ggacttggag tagcttatga taatcttaga gaaaatccac ttaatcttaa
    10141 agagctttca aaattaactc aaaagattgc ttttactaat tcaaacacac taaaatcaag
    10201 acttcttatt gaatcaggac gtttcatagt ttgtaaatca ggtaattatt atgttaaaat
    10261 tgtagatatt aaagtatcaa gagggaaaaa atatcttatt gtaaaaaatg gacttaatgg
    10321 atttatgaga cctattattt ctaatttaat tgaaaatatt acttgtggag tagaattaga
    10381 aggtcaagag ccattatata catcaaatca tgccttttct ctttcagtat taaataattt
    10441 aaaagaagaa gagtatgtaa atgttgttgg gaatctctgt acatcattag atgtaattgc
    10501 aaataatgtt aaattgaaaa aagcaaacat aggagatttg atttcaatta gtaatgctgg
    10561 aagttactcc tattctttat caccgctatt attttcaagt tatgaatttc ctaaacaata
    10621 ttataaaaga aaagatggat atattgagta aacaatagca taaattaata aacttagtat
    10681 tattgaaaaa atttaaaatg cagggggtgt aattctatat ggaattgtgg aaaagaaatt
    10741 tatttgtatg ttggataggt atgtttttta gttctatagg gatgagccaa atagcaccta
    10801 tacttccatt atatattaaa cagttggggg taactgatgt tagtttaata caacagtatt
    10861 caggtataat ttttggatgt acctttgtag tagcagcatt cttttcgcca atttgaggga
    10921 aagctgctga taaatatgga agaaaatcaa tgcttttacg tgcaagtatt gggatgggaa
    10981 tagttatatt taccatggcg tttgttcaaa atgtttatca gttattagga ttaagaatac
    11041 ttcaaggagt ttttacgggt tatgctactg catgtactac tcttattgca acacagacag
    11101 ataaaaatca ttcaggatgg gctttgggta ctcttgcaac tgcatcaact actggctcat
    11161 taattggtcc aacagtagga ggatatatag aatcaatctt aggtcttaaa agtacattta
    11221 ttataacagg aggattgttg tttgtatcat ttatcatctc tattttattt gtagtagata
    11281 attttaaacc taaagaaata aaagaatcaa tttctatacc taaagaacaa ttaaatatat
    11341 tgccaaataa atttttaatt gcatctattt ttgtaaccac attcattact caactagctt
    11401 tatactctat agaaccaatt gtaaccatat atatatcaca acttacaaat tttgcatcaa
    11461 atgtagcttt aatagctgga ttgacatttt ctgcatcagg attagcaaat ttattagcag
    11521 ctcaaaaact tggaaaaatg tcggataaaa taggtcctca aaaagtattg ttaatttcac
    11581 ttttatgggc tgggattata tttataccac aagcttttgt aaaaactgca tggcaactga
    11641 tgttgttgag atttttatta gggttgtcag ttgcagggct taatccttct gttaacagct
    11701 tgcttaaaaa aattgcacca gaagaatatg tgggaaaaat atttggatat aatgcatctg
    11761 cccagtatat aggctgttca tcaggagcat ttttaggggg acaaatatca gcacatttag
    11821 gtattagaac agtatttttt tcaacaagtt tattgttgtt taggaatgca ctttggatgt
    11881 ataaatttac tggattgttt attaaagata agactaaata atcatactat ttacatttct
    11941 aattttatga tgtaaaataa atatttttta tgtaatttat catagaatta tataaaaaat
    12001 aaccctatgg taaaaattgg aaaatattat tatcgacaaa atctttgact taaaaactat
    12061 attgcgatag tattaattta ataaaagttt gagaggggcg taaggttatg acaatttcaa
    12121 aaataccaca agcagaatta aaagttatga agtttatttg ggaaaaaaat gatacagtaa
    12181 catcaaaaga aactattgaa gctatggaac gaaaatatgg ttggaaacaa acaactacat
    12241 tgacactttt atcaagactt gttaaaaaac attttttaag tgcagaaaaa atagatagat
    12301 acactcatta tacaatagtt gttggacata aggagtattt aagtgttgag acaaaagatt
    12361 ttttaagcaa tatacacgac aattctttac agagtttaat ttcagcacta catgatgatg
    12421 aagttgttga taaagatatg ttggatttat ttgaaagtca ttttaaaaat ttagaagaag
    12481 agtaattata tcaggataga actctaagaa tttaattaat ctatggctga aaaccataaa
    12541 tggtattatt taatcagcta ctcactattc ataaagtaat attaaatagg ttctctaatt
    12601 agtaaaatgc ctaaaataat tgga
//
LOCUS       DAELNY010000112        12364 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.112, whole genome shotgun sequence.
ACCESSION   DAELNY010000112 DAELNY010000000
VERSION     DAELNY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.112"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            459..821
                     /locus_tag="KPE55_002528"
     CDS             459..821
                     /locus_tag="KPE55_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0493582.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            886..1503
                     /locus_tag="KPE55_002529"
     CDS             886..1503
                     /locus_tag="KPE55_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG0493583.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLKEILVRLGLEV"
     gene            1500..1985
                     /locus_tag="KPE55_002530"
     CDS             1500..1985
                     /locus_tag="KPE55_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG0493584.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            2028..2546
                     /locus_tag="KPE55_002531"
     CDS             2028..2546
                     /locus_tag="KPE55_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG0493585.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKIIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2682..3641
                     /locus_tag="KPE55_002532"
     CDS             2682..3641
                     /locus_tag="KPE55_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0493586.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3667..5370
                     /locus_tag="KPE55_002533"
     CDS             3667..5370
                     /locus_tag="KPE55_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG0493587.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5583..7685
                     /locus_tag="KPE55_002534"
     CDS             5583..7685
                     /locus_tag="KPE55_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG0493588.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKEG
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7698..8138
                     /locus_tag="KPE55_002535"
     CDS             7698..8138
                     /locus_tag="KPE55_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG0493589.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8174..8977
                     /locus_tag="KPE55_002536"
     CDS             8174..8977
                     /locus_tag="KPE55_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG0493590.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9017..9613
                     /locus_tag="KPE55_002537"
     CDS             9017..9613
                     /locus_tag="KPE55_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG0493591.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9719..10582
                     /locus_tag="KPE55_002538"
     CDS             9719..10582
                     /locus_tag="KPE55_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG0493592.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10649..11638
                     /locus_tag="KPE55_002539"
     CDS             10649..11638
                     /locus_tag="KPE55_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG0493593.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11730..12341
                     /locus_tag="KPE55_002540"
     CDS             11730..12341
                     /locus_tag="KPE55_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0493594.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5013 a         1216 c         2315 g         3820 t
ORIGIN      
        1 atataaaaaa taatttataa agctaaatct aatttaatcg ataatacata tagagaaata
       61 taattaagta aataaacaac tatctattag atattgagaa agtcaaatcc tatatgaatc
      121 tacagaaatg attttaatga atctgaaaat tttttttata caaacataaa aaaggtataa
      181 agttgtttta ttataattta attacttttg ataacttttt atgttttata caattatatt
      241 tttaataact cgtcaaaacg ccaaacatat attacaatag atatataagt ttttttgttg
      301 acaaattatg ttaagagaat taagtataat atggcataaa taattacttt tatttatcaa
      361 tgaaatgtat ttattgtgta gcgaaaaata gtcagatagt atagacaata actgaagata
      421 aaagcgttta ttgttgtaca tatggaggag ttagttttat ggaaaataaa attttgatag
      481 tagatgatgc tatttttatg agaatgataa ttaaagaagc tctgagtaaa agtggctata
      541 ataatttaat agaagcaaat gatggagaag aagcgtgtaa tatatttaaa tatgaaaagc
      601 ctgatttgat gttgcttgat attacaatgc caaaaaaaga tggtttagag gtgctaagag
      661 aaataaaaaa gttggattca agtgctaagg ttgtaatgtg ctctgcaata ggacaagaga
      721 atatgattgt agaagccata aaacttggag cgttagattt tatagtgaag cccttcaaaa
      781 cagaaacatt agttaaagtt gtagataatg ctttaaaata aaagtgtaaa tatacactaa
      841 tacaatgaaa agcaaataaa tgcaataata aagggagggg tttatattat aaattattta
      901 gaacttgatg atgttcatat agatgctctt agagaaattg gtaatatagg ctctgggaat
      961 gcaattactg cacttgcatc catgataaat agcaatgtag aagtattaat acctatggtc
     1021 aagatacttg aatataatga agcgacaaat ttgcttggtg gtccagaaaa caaagtagta
     1081 tgtattttac tcgatatgaa aggtgatatc aatggaatgt ttatgttttt gttggatgaa
     1141 tcaataactc aacttatgct aagttctctt tttaataaag aagaagcttt tttagatgag
     1201 attgaggcaa ttgaaatttc agcaattaag gaaataggaa atattatggc aagttcatat
     1261 gtaaatgcta ttgcatctat gttaaacatg actataagtg tttcaattcc agatatatgt
     1321 attgatatgg ttggagctgt tctaaatgta cctatgataa gattttcaga tgttggtgac
     1381 aaagtcttat ttatagaaaa taaatttaaa atgagtgata attattttac tagtcacatt
     1441 ttgatgattc cagaaatgag ctcacttaaa gagatactcg tgaggttggg attagaggta
     1501 tgaatagaga gatagtagtg aacattgcag atatgaagat agcctataga ccaaatgttt
     1561 tagttactta tgcacttggt tcttgtgtag gagtatgttt gatagataaa gtagctggaa
     1621 ttggtggaat gttacacgtt atgttgccat atagtaaaga tgctattaac attgaaaata
     1681 agtgtaaatt tgctgacaca ggaataaatg agctcattaa atctatggag aatataggtg
     1741 caaatagaat atatataaaa gcaaaaattg ctggaggtgc tcagatgttt ttaggaagta
     1801 gcaattcagt aattgcaagt ataggacata ggaatgttga ggcagtaaaa aaaactcttt
     1861 taggattaaa tattccaata ttagctgagg atacaggtat gaattatggt agaacaatac
     1921 gtttttacac tgagacgggt gaattacttg tagattcaat aaataaaggt aagaagaaat
     1981 tataatatat atttcataga aagtgtattt taggagggag gtattaaatg agatttccag
     2041 atgacccata tttagattat ggactaattg atgaaggaat taagtatcta aagtttaaag
     2101 tagatgagca agattttggg gttgaacttg aaaagataat agaaattact ggaaatcaag
     2161 aaattatatt tattccagaa ctacctacat attctaaagg cataataaat ctaagaggaa
     2221 aaatcattcc gataatagat atgcgtttga gacttaaaaa agatgaatta atgactacaa
     2281 attgcatgta tatagtagta actgaaataa aggacttggt agttggattt gtggttgata
     2341 gagttgatga tatagtaagc ttagaaaaaa gtaaaatatc acctccacca agagttacta
     2401 tagaatattc ggattatttt gtaagtggag ttggagaatt agaaggtaaa ataattactt
     2461 tactagattt agagaaactt ttgacagata aagatgaagt tttaatagat gaattaatca
     2521 atgggtttga ttgcactatc atgtaatata gggatgtagt tggagtgtta ttataaaaag
     2581 attgtgatga gaacatttaa gaatatagtg gtataattat taagtataaa aattttaaat
     2641 cattaaaatt tgaatatatt atgtttaaga gaggtttgta tatgaaaaat aagcaacagg
     2701 gaacaatttt agtcgttgat gatagtgttc tgatgtgtga cttaatagat aaaacactta
     2761 caaaagataa ttttaaggtt aaaaaagcat ttaatgcaat agaggcaaaa gaatttattg
     2821 aagaatttat accagacatt attttgcttg atataatttt accagataat tctggttttg
     2881 agttttgtaa ggaagtaaaa tctaatgctc gtacagcaga tatacctata atttttataa
     2941 cttcaaaaaa tacagataca gacatagtaa aaggatttgg tgtaggagca attgactata
     3001 tggtgaaacc ttttagtatg acagaattaa aagctcgtgt tatagctcat cttgaagtaa
     3061 agaaatctca agataaacta aaaaagataa ataatgaact ggaatcttct cttgaaaaat
     3121 tgaatagact cgttgtaaga gattatctta caggtcttta caataggcga catataatta
     3181 atcagcttat ggagcaaaga agaattaata aatatagtga aacatcaata tcccttatcc
     3241 ttggtgatat tgatgacttt aaggttatca atgatactta tgggcatgaa gcaggggatt
     3301 tcgtgttaac agtaatatca agtataataa aaaagaattg tagacaaatt gatactgtat
     3361 caagatgggg tggagaagag tttttgataa tgctcacaaa tactccaata gaaggtgcta
     3421 agatactttc agaaagaatc cgtaaagaga taaaggagtt tgatttcaac tataaagaac
     3481 ataaaataaa gtgcactatt acattaggag tagtagaagt aaactcaaat atatcattag
     3541 aagaaaacat atccttagca gataaagcaa tgtatgaggg taaaaactta ggaaaagatt
     3601 gtagcgtagt gagtacaatg aaaggattaa aaaagatata aacttacttt atgaaggagt
     3661 acagatatga aaagagaagt aaagctttct aaattagcaa ttgtattgct gattattata
     3721 gtattggctg ttctagcagg gattttttct tctatttcat cttataaagc atttgaaaaa
     3781 gtagaaaaaa gtattaatga tatgaataat ttgaggactc aaagtcagta tataaaagat
     3841 acaactagag agataagtga tgtattaaga agatatgtat ttacaggaga aaaaaagaat
     3901 aaagatattt atgaaaaaga attatacgga aaaacaagag aatcagcttt aaacaaatta
     3961 gaaaaaattg gtcttacaaa gagcgagtca aataaaatct tagaagcaaa agaaatatca
     4021 gattctttag taccaacaga acaaagtgca ataaaggcag tcgaaagtaa tgataagagt
     4081 aaagctcaaa gtataatttt tggtagtgac tatataaact cccaagagaa aatggattct
     4141 ttaataaatg aatttcaaga ggatatagtt acccgttcaa attccaaaat tgtagaagta
     4201 aaaaaagaat tacaaagtct aatattaaga acaatattta tactttccct tttaataata
     4261 ttgacaataa tagaatatac agtaataaaa ttaaaaatag tgattcctat taataaagta
     4321 gaaaaacact tttcaagaat tgcatctggg gacttaagaa caagtataga cgttgaagaa
     4381 agtaaatctg aaataggact attagtgagt tctgtcaaaa agatgcaaag tatgctttta
     4441 aattacatag accttataga cagtacatta actagtattg caaaaggaga tttaagagta
     4501 gagatagacc aagaatttat aggtgatttt aaatcaatta aaatatcttt agaaagtata
     4561 ataaactctt ttaatggtga tttttctgaa ataaatttat cagcagagca agttgcaagt
     4621 gcttcagaac aagttgcagc aggtgcacaa gctctttcac agggtgctac tgaacaagct
     4681 agttcagtag aagaattagc ttccacgatt aatgagatat cagatggaat taagaagtgt
     4741 gcagatagtg ctagtcttgc atgtgaggta tctgaccaat ctgcatcaca agttggtttc
     4801 ggaagtagat gtatggatga tatgatgaca tcaatgggtg aaataagtga aaaatcgagt
     4861 gaaatatcaa aaataatcaa aacaatagat gagatagcat tccaaactaa tattcttgca
     4921 ttaaatgcag ctgttgaagc agcaagagca ggtcaatatg gtagaggatt tgcagttgta
     4981 gcagatgaag ttaggtcttt ggctggtaaa agtgcaaaag cagctcaaaa tacagctatt
     5041 ctcatagaag aagctattaa aacagtagaa aatggtgtca gaattgctaa agagactgca
     5101 aatgcattgg acttggtagt aaatggtgta gaaaaaacta ctaattatat taatggtatt
     5161 tcttcggctg tacatagtca agaagtagca attacacaga ttttatctgg aatagagcaa
     5221 atttccatag tagtccaaat gaactcagca acagcagagg aaagtgcagc agcctcagaa
     5281 gagatgtctg ctcaagctca aattttaaaa gaattagtag aaaaatttca tctaaaggaa
     5341 agtgaagtaa gagaatcatc atgggtttaa attatgaggt gaatattgaa gtaagagaat
     5401 tatcatgtat ttaaattatg agatgtatat atattgaagt aagagaataa ttgtgtattt
     5461 aaattatgag gtacatatag tgagataaga gaatcatcaa ggatttaaat tatgagatgc
     5521 atatataata gaaaattatt tgtaggtcgt aggtgataaa tgtgaattat attgaaccga
     5581 gtatgagaca aatgttagat atgtttatat ttgagacaag tttattactc gaacaattag
     5641 atgaaattat gattcaaata gaaaaagaga aagtatttac tcataacaac attaatgaaa
     5701 cttttagaat aatgcatacg ataaaaggtt cagcatctat gatggggtta gacaatatat
     5761 ctgctttagc acatagtgta gaagacttgt tttatgctat acgtgaaggt aaaatagaag
     5821 ttttagacaa tgacctatta tttgatttag tctttcaatc attagatttt ataagaagag
     5881 atatagatga gttacaatca agaggttgta tatcttctga ttactctaat tttattgaag
     5941 gaattgaaga gtatgtgagt actttggaat tatcaagttc tatgaaatca aaggataatg
     6001 caaatttaga aagtacagac gaaaaagaag taatagattc aaattttgaa tacaaagaag
     6061 gctctgatgt agtaaaaata atctttgaac aaggttgcat gatggaaaat gtgagagctt
     6121 tcatgcttgt atttgaactt agagattact gtgaatttat agatttttat ccaaaagatg
     6181 tagaaacaaa tgcagagtct acagagtata ttaaaagtta tgggttttat atacaatttg
     6241 tagctaaaga ggataaggca atagtttata aaactataga agataatctc tatgtaaaag
     6301 aatacataaa tattagtaaa aaagaaatag aaatgaaaaa tacatcaaac aatgatgcag
     6361 tagaaaataa attaaaagat aataataaag taagtaaaag ttatcaaaat aactatacag
     6421 aagatgagat acaagaagat gttttaaatg aaaaaactga tatggaaaaa gatagtctaa
     6481 gtgaagaaaa tgaacattta ccaataagta agcagagtat tatgagtgta aatttagaaa
     6541 aattggactt acttcaaaat atagttggag aaattgtaat tatggaatca atggttgtaa
     6601 atagcgtaaa ttctacaggt gttgatatag ataatttcaa taaggcaaca agacaattac
     6661 acaagcttac agatgaattg caagatatag taatgtctat gcgtatgatt caaattgctg
     6721 gtgtatttca aaaaatgcat agaattgtac gagatatgaa taaaaaatta gataaaaatg
     6781 tagagttaat aactatagga gcagagacag aggtagataa gagtgtaatt gataatttag
     6841 cagaaccttt tatgcacctt ataagaaatg ctatggacca tgcaatagag cctattgagg
     6901 agcgtatagc gaaaggtaaa gaagaagtgg gaaaaatagt tttatctgct gaaaatctag
     6961 gcggagaagc tgtgataagt atttcagatg atggtagagg cattatgaga gataagattt
     7021 tggcaaaagc taaggaacaa ggtcttctta aacatcaaag taatgagtat actgatgacg
     7081 aggtcaattc tttgattatg actccaggct tttctacaaa agaatctata actgaatttt
     7141 ctggaagagg agtaggcatg gacattgttc gtaaaagtat tgaaaaagtt ggaggttcaa
     7201 ttgaagtaaa aagtaaacag gatgaaggga caacctttac tataaaaata ccattaactc
     7261 tttctatagt agagggtatg aagtttaaag ttggagattc actatttact ttaccaatat
     7321 catccataag aagaacattt aaacttacag acttaaatca aataattaca gaaggaaata
     7381 agacagagtt aattacaata tatggagcat gtattcccat tataaggtta cataaaatat
     7441 ataatattca aacaaaagta accaatctga tgaatggtaa tatgataatt attgaaaatg
     7501 agcataaagc agtatgcatt tttgttgatg aaataatagg taatcagcaa gttgttgtga
     7561 aaccatttcc agtttatttt aaccgattta atataaaaaa taagggttta tcgggatgta
     7621 caatacttgg ggatggaagt ataagtttaa ttatagatgc agatagcatt atagaaatat
     7681 attaggggat ttaaagtatg gctaaagatg aaattattaa acaagtatta acattttatg
     7741 tcaatgatgt tatttatgga atagaattag aaaatgtgat tgaaaccata agatttcaat
     7801 caataacgta tgtaccatgt cttccaacat atataagtgg agttattaat ttgagaggaa
     7861 gaataatacc agttattaat atgcatataa agtacaatct tcctaaagct gattataatg
     7921 agagaacttg tataataatt acaaaggttg atgagtatca agttggaatt attgtagata
     7981 aagttgttga tgttattcat atagaaaatc taaatttact agagacgact aattctaaca
     8041 acactaatat taataaagat ataaaagaga ttgcgaaagt tgaagataat tctatattaa
     8101 ttttagatat tcgaaagttt ttgattaata gcatatagtt agttctgata aattagcagg
     8161 aggaagtttt tgcatgataa aactcacaga tgaagaattt atagttcttg taaaccatgt
     8221 aaaaaaagaa tatggtattg atttaagtaa aaaaagagcg ttaattgaag gtcgcttata
     8281 caatacaatg atagagaaaa aacttagttc ttttagtcaa tatatgaatc tattatttaa
     8341 agataagaca ggaaatgaag ctataaacct aataaataga ttgagtacta accacacatt
     8401 ttttatgaga gaacctcaac attttgagtt tatacagaat agtatacttc ctttttggga
     8461 agaaaataat aaaataagaa acataaatat atggagtgca ggatgttcta gtggagaaga
     8521 agcgtattca atagctatga ctttggatga ttattttgga tataataaag agttgtggaa
     8581 cattaaaatc atagccacag atatttcaac taactcactt gaaaaagcta aaagaggtat
     8641 atacatagaa tcaaatgtta acaatgttcc tgagttatgg aagaagaagt attttataga
     8701 caacaaggat ggtacattta aaatttgtga taagattaga aaaagtgtaa tttttaagcc
     8761 gtttaattta atgaatgatt tctcatatca acattttgat ttgatttttt gtagaaatgt
     8821 tatgatttat tttgatttaa aaacaagaga agaacttata aataaatttt ataatgctac
     8881 caaaaaagat agttttttgt ttgtaggaca tgcagaagta atcaacagaa gtagtactaa
     8941 atacaaatat gtaaaacctg ctatatataa acgataaaaa ggtatttttg atatatgatt
     9001 ttttggaggc ggtaatgtgg aacgttttaa taatatggtt attgctatag gcgcatctgt
     9061 aggtggtaca gaggcaatac ttgaaattat aaaagaccta ccaaaatcaa caccagggat
     9121 tgttgtagtt caacatatgc cagctatatt tacttatatg tatgcacaac ggcttgacaa
     9181 gcagtgtatt atgaatgtaa gagaagctaa gaataatgat agagtagaac aaggaaatgt
     9241 gttaattgca cctgggggtt accagatgaa attgtgtacg gataaacaag gatattatgt
     9301 aacttgtgaa aaaggagaaa gaataagtgg tcactgtcca tcagtagacg ttttgtttga
     9361 ttcagtagca gaagtagcag gtaaaaattc aataggaata atacttacag gtatgggtta
     9421 tgatggagca agtggacttt taaaaatgaa agaaaatggt tcatttacaa ttggacagga
     9481 tgaaaattct tgtatagtat atggaatgcc aatggtagct tttgatagag gttctgttat
     9541 gttacaatta cctcttggtg aaatttcaaa ctgtttgata agacatatta atattttaaa
     9601 acaaaaagat taatttttta taaataaaaa ttaatctttt attaaattat atggataaaa
     9661 aaccctagaa actccaaata taataagaga gatttatatt taggaggaaa atgataatat
     9721 gaaatttttt aaagtcgata caaaagctaa agttttaaga tatgtatttg ttttaacatt
     9781 gactataatg gctgttttgg caattaaaca tgactataaa tataataatt taaggaaaga
     9841 tgcttcaatt aatttaaata gtaataagga agcagtgact gttatgagta accagtcatt
     9901 accaaagggt aatacaaata gtaaacaaac tttatcagta agtacaatag gaagtaaaac
     9961 aatagtgaaa aatgatgagt atttagaaag tactataaac atgcctgtaa taacaaattc
    10021 aaataaaatt gttgaaaggt ctacaaatga taagataaaa aatgacatat ttgaatttta
    10081 taataaatca tataaagaag caaagcagta tcttaaggat aatcctgacg agaaaaataa
    10141 gtttgttgca aatgtagact ttgaacttaa aaagaatact gattctgctc ttagtataaa
    10201 agtgagatat tatacttata gtggtggagc acatggattt tatcaagata tagcatataa
    10261 tgtggatatg agaactggaa aatttttaga attaatggac ttgtttaagg ataatactaa
    10321 atataaagaa gttatagatg aagaaattaa aagacaaata gctgaacttg aaaaaaagga
    10381 tgaagaaaat atcggaatat ataattttaa aggtataaaa gagaatcaaa acttttactt
    10441 gcaagatgag aatctagttg tatattttga tttatatgat ataactccat atgcagcagg
    10501 aataccagaa ttttcaataa ataaagcgtt gataagttct atgctaaaac cagagtatgt
    10561 agatttattt gatttgaaat agaagattaa attaaatgtt atgctataat atttagcata
    10621 gagacttatt aaaattgggg gtataaaaat gctactttta aaaaaagacg acataaaaaa
    10681 agtatttaca atgagagatg caattgaagc tgataaggaa gcttttagaa tttattgtga
    10741 aggaaagagt gtaaatcctt tgagaaccaa tatatctgtt ccaagtcaag atgctagtat
    10801 gttatttatg ccaggatatg tagaagaatt aggctgtggg ggaataaaaa ttgtatctgt
    10861 atttcctaaa aatgctcaaa aaggcaaacc agtaattcca gcaacagtac tattattaga
    10921 tggagaaact ggagaagtta gtgcagtatt ggatggtact tatgtaactc agattcgtac
    10981 tggagcagcc tctggagcag ctatagatgt attggctaaa aaagattcta agattggagc
    11041 tttgataggt ctaggaggac aaggtgagcc acaacttgaa gccatgatag aagctagaaa
    11101 tttggatgag gtaagagttt ttagtagaaa caaggaaagt agagaaaaat ttgcagaaga
    11161 aatgaactta aaattaagta aatataatac taaaatagtt gcagttgaat catctgatga
    11221 agccatagat aacgcagatg tgatagtttt agctactcca tcaaaacaac cagtacttaa
    11281 tggaaactta gtgaaaaaag gagctttaat aagtgcagta ggttcatata tgccagatat
    11341 gcaagagttg tgcccaaact gtttaactag agcgtctaag atattttttg aatcaacaga
    11401 tgcagtttta tctgagtcag gagatatatt aattccatta aaagaaggta agataagtaa
    11461 agatgatttt agtggtgatt taggaaatgt tataaacgga actattcctg gaagagaaga
    11521 tgatgatgag ataattgtat ttaaaactgt tgggataggt gtacaagatg tcgtaacagc
    11581 aaaaagaata tacgataaag caaaagaaaa cggtattgga atagactgga agtggtaaaa
    11641 tattcaaaat aattctatag ttagacaaat ttatgttata atgttgataa ataaattttt
    11701 tattatttta attgagggag gatgtttgta tgaaaatggt aaaaaagtta atattatcta
    11761 cgattatggc tctagtttta gttctaacta tagggtgttc taatgcagaa gaaaaagtaa
    11821 agtcgaattt tgaagctaat ttagctgcat tagaaatgta taagtatgat gaaaaaggaa
    11881 tttctgatgc attaacagaa gctgaatata aacagattca aacttataac agcaaggaca
    11941 aggtttcaat gattgctcaa ttgacacctc tttatggtaa agaaaatgct gaaaagatat
    12001 acgaaacaat tgttaaatta attcaaggtg ttgaaaagac agtaacagtg gaatcttcta
    12061 gtaaggataa gataactttg aaagttgtaa gtaaagcttt agatactagt aaagtaatga
    12121 gtgaagcgaa cactttagta aaatcttatg taactgatga aaagaattac gataaaaaag
    12181 aaaaagaagt tcaagatgat atgacaaaaa tgattataga cctttacaat aagaaaccaa
    12241 ctaaggaagt tacaactatg attacatata ctaaaaaaga tggtaaatgg caaccaccta
    12301 gtaatataca aaagttatgt gaaggaatat atacaattta atttatttta aagacacaaa
    12361 acta
//
LOCUS       DAELNY010000113        12241 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.113, whole genome shotgun sequence.
ACCESSION   DAELNY010000113 DAELNY010000000
VERSION     DAELNY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.113"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(574..846)
                     /locus_tag="KPE55_002541"
     CDS             complement(574..846)
                     /locus_tag="KPE55_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493595.1"
                     /translation="MLNHINKILLILSLFTPIMILKSILTYDKDTDKTKKISFFTVLL
                     AYLITKIIILFNVDKTHDYNMKKNLYDIIYYLIFITWELTIIIFIR"
     gene            complement(840..1085)
                     /locus_tag="KPE55_002542"
     CDS             complement(840..1085)
                     /locus_tag="KPE55_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG0493596.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYVEN
                     IKSNTAILDSQEEVPISRYRYKDIKERFLKFIGDRIC"
     gene            complement(1402..1554)
                     /locus_tag="KPE55_002543"
     CDS             complement(1402..1554)
                     /locus_tag="KPE55_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493597.1"
                     /translation="MKNLNKNTILGEVIITIAACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            2163..3629
                     /gene="dndC"
                     /locus_tag="KPE55_002544"
     CDS             2163..3629
                     /gene="dndC"
                     /locus_tag="KPE55_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HBG0493598.1"
                     /translation="MAVNYLDNNNLENLYDEMQAVYLNDDRPWIIGYSSGKDSSCVVE
                     LTFRMLKRLPKEKRHKEIYVISSDTLIENPIILDYLKNNIDKINESATEHDLPISAQI
                     VYPELNDSFWANIIGRGYPTPKSIKYRWCTERLKIKPSNKFIKEKLEQKDVIVLLGVR
                     KEESSARKSRIEKREIEGYLLTPHETLRGKNKLAYVYTPIVNFSTADVWDVLYHQNDN
                     FIDFGGEFGPSPSTSWGTDAMELFQMYMKGSGDSDECPFIADEASAKSSCGNSRFGCW
                     ICTVVKEDKSLNGFIESGHDELKPLLEFRSWLISERDKPKNRKKHKRNGSVIKKDGRI
                     MFGPFTFEARQTILRKLLETQLEMQKFYPDLQLIQLGELKAIDDIWDNEEDLTGNILS
                     KIYKEVVGKNLPWSEYKKPVFDDLTLNIISEKCSEYDINIDLFNKLMIDTNKYKYFSN
                     NTKLKTSIERILNQQWLHQDIIEKIEEESNKELKYENK"
     gene            3616..5775
                     /locus_tag="KPE55_002545"
     CDS             3616..5775
                     /locus_tag="KPE55_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0493599.1"
                     /translation="MKINKLVLKNFRSYEEETTFNLNTTSEKNIILIGGKNGAGKSTI
                     FEAIKLCIYGPIAYKYQGFNSSYISRVKSNINNNSLRNSKVDTLVSVDIEISEGTEKN
                     TYTLIRQWTFKDKKLNETFSVYKNFSFTPLNADELNYFENYITSIISPKIFDFFFFDG
                     EHLYDFFIGKNSNIHLKESLLSLCNFDTFDILKKMLISTNRSNKNVSDEINIIKDDYL
                     NLEVEINNLNSQEKNLSYELEKISSEIEKLQQEQLKIKKEFRKKGGILAEEREMLNSR
                     IAELEGNRFIINQNIKDFCNDILPFLIVKKQILNLEKQLKVENDSILYSHLKDKLNIE
                     YIKNLLSDKIMSSSLDEIALTVSEALIEDIKPDSYEDNFESIHKLSNDESSKLLSLIN
                     TILNFDNNSIINSFGEHRSISNELADIRHKLNTSFKDETLNTYINDISSLSDKIDSLL
                     KSKNLLVTSLDKLRLEIAETITKRDKSKAKYIYLLQHNNIVDISSNLILMLDEIITTL
                     TETKIKLVQDNFMYIFKRIIQKDNFISSIDIDANFNISLYINKIYNSLELENLVENIG
                     SEEMEKKLGKLFFEDLFNLYRVKDKDDLFNAIKNNTQSTFLNLRTKVDIDEFSSGEKQ
                     IYILCLYWALLKSSEIEIPFIIDTPYARIDDTHRNNITSEYFSTISDQAIILSTNTEI
                     DEKSYKIIKPKLSNEYLIEYDNKNKKTIQNKGYFFEV"
     gene            5778..6161
                     /locus_tag="KPE55_002546"
     CDS             5778..6161
                     /locus_tag="KPE55_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HBG0493600.1"
                     /translation="MGFRLKTSIQTKEIFEELGSRMNLKPFALCKLAISISLSNSEPI
                     ENYESIDNNGLELNRQTITNQYDTLFKTLLEQHAGRHLSDDEYFPNQVKLHIDRGALM
                     LKNRYNYSSNLEHFITQIVQENNNL"
     gene            complement(6271..7413)
                     /locus_tag="KPE55_002547"
     CDS             complement(6271..7413)
                     /locus_tag="KPE55_002547"
                     /inference="COORDINATES: protein motif:HMM:NF014044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0493601.1"
                     /translation="MKIIFGNIKNNNKELIWDPTNSIKVPHPNTGIIGTMGTGKTQFT
                     KSLVTQIHREQKHNINGKPIGILIFDYKGDYIKDDFVDATSAKVYEIYHLPYNPLTLI
                     IPERIKPLLPVHTANSLKETIAKAFGLGPKQEVILRELIMKAYEKKGIKKDDENTWVK
                     SCPTMQDVLSIYLDDREIKKDDKLYAALSSFNDFEIFEPNSEKTKNLFDLINGVTVIN
                     LSYYDSGIQNLVVAITLDLFYSQMKALGSSDITGNGEYRQLNKMILVDEADNFLSRNF
                     TSIKNILKEGREFGVGTILSTQLLSHFSTSENEYQNYISTWIIHNVSDINNKDIRYIF
                     NSKNKSEEDNIYNKIKNLAKHESLVKMPSENSPIYIRDKAFWELNK"
     gene            complement(7423..11394)
                     /gene="dptH"
                     /locus_tag="KPE55_002548"
     CDS             complement(7423..11394)
                     /gene="dptH"
                     /locus_tag="KPE55_002548"
                     /inference="COORDINATES: protein motif:HMM:TIGR03237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HBG0493602.1"
                     /translation="MSDQFYRYLAKNLVKYFESREIKSGDKFNIQFEREEQVKIMYDV
                     LEKESNSRPFTYNIMNGSKYQTFSMNVSGIQLIVASNNNTTNDFLIRLRNLIGNVASD
                     EFKDSAILFIHNMTLDSLIRGTESLQKEGMPFHTKTLVKDIQNKLENDKLKEDIKHIL
                     YFVLDRMNKSILEDNSSIFQYENILDILNKGRIEKSEYSKFGLFYDDNMLDNKSSKAI
                     KERLLDNEKLFSIVNSIHQYGDPKLELEKHFDSKGIDLLKDENWKYSTYKDIKSSQER
                     MKNKKVELLCIECVENLKLWSKPDIENKSKRKNILLFNKDKLDIINLCIKFDKKIEKT
                     SFSIDKMSKTYTEVKASGNKAYISININISETTFTKVKYNDGIIKYEFRICIVSCDEN
                     ILNDIKSNYGIVINSKRSMLVTNTDDDTIVINPKGIIERKIQIDEFENEINLEEGERL
                     DLELNVELEEDVEFIKIDVVYDQSVITIVKQEEKEQPISISGMKTWKLKRERKESFKY
                     LGNSKIVQGTSEYFPKENFKENLQIEKYIIENNIMHCTILEEKYKIIELDVDSRIKEA
                     YKNLISYYKVNDCIPSLAYYDVDLVNISKNYINTILEVFEDIKNGKVLSKERLNIAKV
                     GTIHRIDKEEELFLTPLHPVNVAYQLMINNVIGDEEVKEGILKLLGSKYLMPYIYKGK
                     NSLYKVVDQRHSPEWTYYVDHDMSRYKGSRDYVSKLTKEKINEFIEHFTYLFRDISKS
                     VMKIKLINLGDCKEVLLGIFDYYIKKLGKGEEINNLISIDIYIYGDKSIINSFEEIAF
                     YNSINDIKKHFNVNLSVDNYSEEDVLNLFRDKVKFYKKDKMSDLYEYSHLTLYQMDKD
                     VNITSSSMDDIKTGVALNGLIAGVPSIYLEDTYRTGFGSKFMATTKNELLNISMKFNS
                     LTKSIGTGDPFNDNLSVTTSISENNSSFLNKIYKTSHWVTFIDPKVDLNFFKDDENSN
                     DLLIIHYSDQYTTSSGYDAITVTRKSEQYKFIIEEFLNSKDIKVEEDTIPNLINYFNA
                     INGDWLLRLISSKSQFPREKLSILSAIKISLAYFYHPNIIWIPISLEEILRVSGGAGL
                     KQAGGLFTVKKLTGDTGSYSDDLLLVGIENKNNKIKVYFYPVEVKIGDNNESVIKKAI
                     IQANKTRDLIEEYLTESENDSQKFTKLMYRNFMIQLAIVSAQKMKLYHIWDEQNWDDI
                     INSDIRTNLLNDNYEISNDLDEFIGRGAVISFKKGTYYNGNLGKEGNITILNLSEQEG
                     YNNIVMDLEEMKEKYIRGESDFVIQEMLINKYLDNTIGIQNMDKNVILDTNVMLNKTK
                     DYD"
     gene            complement(11375..>12241)
                     /gene="dptG"
                     /locus_tag="KPE55_002549"
     CDS             complement(11375..>12241)
                     /gene="dptG"
                     /locus_tag="KPE55_002549"
                     /inference="COORDINATES: protein motif:HMM:TIGR03236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HBG0493603.1"
                     /translation="YKCICKNVSKLFQEDLEFMLNYPELIINEFERLLKYYYFFYVSQ
                     LSIKLSHFFDGDRDRTEKVYFILSWEKASKTRISYNMGWKMLESHIRILFSHVHTLEI
                     LNTNNDISKYDYVDLNKIIKNLSENERLRLYENIIDIKNTYVKSITDVQWDDYDEQTK
                     VYKNDNIKEEICDLHKRIDYQFKKSSREANYKRYKNLFEEYCKLNFLKKAGPLGNVLK
                     INQEELLFMTKLCMKNKTKIKLKELFEEYEKRGIYFDRDSQSKVVELFEKLNIIEKKS
                     DSGDAQYVRSIL"
BASE COUNT         4285 a         1685 c         1317 g         4954 t
ORIGIN      
        1 caatatatat gattatttat atcatgataa agcttcttaa ctttagcgtg tgattcttga
       61 atacttagat aatgagaata ctgcatatct aacttatttt ttattacttc attttgcaat
      121 tttaacttat tttccttaat tatttttata aacactataa taatgactaa gctactagat
      181 agtattaacc taggtatcac aatataccca actatatata tttcctaata cccaccaaat
      241 tgatacattt ttctatttat ataaattatt aatatagtaa ataaatttgt tattaaagca
      301 acagataagt atatatagtt tattttatta tctttatcta gtcccaatct tttattaata
      361 aatttaaata tagcacaagc tactatataa ttattattat aagataatag tatatatcta
      421 ctttagcatt atcataacta aacactaata gagcttcctc tgatgatatt acattacttg
      481 taactacaaa aaataaattt atagatataa catcttttat taaagggtga tatacgtcaa
      541 ataagaaaaa tgaaactata tatttaacaa ttcctatctt ataaaaataa ttatagtaag
      601 ttcccaagta ataaatatca aataatagat tatatcatat agatttttct tcatattata
      661 atcatgggtc ttatcaacgt taaataatat aatgatttta gttataagat aagctaataa
      721 tacagtaaaa aaagatattt tcttagtctt atctgtatct ttatcgtaag ttaatatact
      781 ttttagtatc attattggtg taaacaatga taatattaat aatattttat ttatatggtt
      841 tagcatattc tatcaccaat aaattttaga aacctctctt ttatatcttt atatctatat
      901 ctgctaatag gtacttcttc ttgactgtct aatattgcag tatttgactt tatattttct
      961 acatagttta agttaattat ataactttta tgacatctta caagttcctc tatatttaaa
     1021 tctttttcta ttttttctag tatacatata gcatcaaaag ttttattttt tgtatgtatt
     1081 agcatgttct ttttttgaac ttctatctat ttttttgcta gagtagattt tgtatgtgtt
     1141 agacttattt ttattttttt caattttttt gatacatgtt aatatgtgtt tttctagttc
     1201 ttgaaaattt attggcttta ataagtatca ataagctctt acttcatatc tttcctgtac
     1261 ataatttatt agagatgtta taaatattat tatctacttt tcttatgttt cttgcgatat
     1321 ctatactatt tagttctggc atttttatat ctaagagtag tgtatcctct caattaactt
     1381 tgtaatggtt cttcttttga gctatatttt tttaattcga tattctttgt ttttattttt
     1441 taatatatta tcaacttgat tttttattac gatttgtatt tctttttcat catcacaagc
     1501 tgctattgta atgatgacct ctcctagtat tgtattttta tttagattct tcactttagg
     1561 ttaacacttt ttaaatgtat ttatcaataa tatttaaatt aaaaatgtat aaaaatacct
     1621 atatccaatt ggatataggt attcgtggat ttcaatggtg gatacgaggg gagtctataa
     1681 gtggttattt gattgttttg ttggtttttg tgaatgtagg tatatagcta tttagttatg
     1741 atattagact gatttttttg gttatttttt attgtttatt tttgtggttg gggtattatt
     1801 ggggtataac ttttaaagtt tataaattga gtcattaata aatatctatt gtttagtcaa
     1861 tctataatat aataaatctt aaatagtgta actattctaa gtagctgcta tttttattca
     1921 tacatatctt tatatacatt tatgagattc aagtatgtac attcttattc tctaattttt
     1981 aaatatatta tcttaaaatg gtaacagttc tacatttaat taaatttata ttttattgta
     2041 cttatgtaaa tataaactaa tctattctct aaatatatat tgtgatataa ttatatataa
     2101 attaaaatag gagtattatg taaagatctt cgctatactc ttatagaaag gatacttaat
     2161 atatggctgt taattaccta gataacaaca atttagaaaa tttatatgat gaaatgcagg
     2221 ccgtatatct taatgatgat agaccttgga taatcggata tagttctgga aaagatagtt
     2281 cttgtgtagt tgaacttaca tttagaatgc ttaaaagact acccaaagaa aaaagacata
     2341 aagagattta tgtaatctca tctgatacac ttatagaaaa tccaataata cttgattatc
     2401 taaaaaataa tatagacaag ataaatgaaa gtgctactga acatgattta cctataagtg
     2461 cacagattgt atacccagaa cttaatgata gtttttgggc caatataatt ggtagaggtt
     2521 accctactcc aaagtctatt aaatatcgtt ggtgtactga aaggcttaag ataaaaccat
     2581 caaataaatt tataaaagaa aaacttgaac aaaaagatgt tattgtactg cttggcgttc
     2641 gtaaagaaga aagttctgct agaaaatcga gaatcgaaaa aagagagata gagggttacc
     2701 ttcttacccc acatgaaacc ttaagaggta aaaataagtt agcttatgtg tataccccta
     2761 tagtcaattt tagtacagca gatgtttggg atgtactcta tcatcaaaat gataatttca
     2821 tagattttgg tggtgaattt ggaccatctc catctacctc atggggaact gacgctatgg
     2881 aattatttca gatgtatatg aaaggttctg gtgactcaga tgaatgccca tttatagcag
     2941 atgaagctag tgctaaaagt agctgtggaa atagtaggtt tggatgttgg atttgtactg
     3001 ttgtaaaaga agacaaatca ttaaatggat ttattgaatc tggtcatgac gaattaaagc
     3061 cacttttgga attcagaagt tggctaattt ctgagagaga taaacctaaa aatcgtaaaa
     3121 aacataaaag aaatggctct gtcatcaaaa aagatggtag aatcatgttt ggaccattta
     3181 catttgaagc aagacaaact attttaagaa aacttttaga aactcaactt gaaatgcaaa
     3241 aattttatcc tgatttacaa cttattcaat taggagaact taaagctata gatgatattt
     3301 gggataacga agaagactta actggtaata tattatcaaa aatatataag gaagttgtag
     3361 gcaaaaacct accatggtct gaatataaaa aaccagtatt tgatgacctt actttaaata
     3421 ttatatctga aaaatgtagt gagtatgata taaatattga tttatttaat aaattaatga
     3481 tagatacaaa taaatacaaa tattttagta ataatactaa attaaaaact tccatagaaa
     3541 gaatattaaa tcaacagtgg cttcatcaag atattattga aaagattgaa gaagaaagca
     3601 acaaggagtt aaaatatgaa aataaataaa cttgtattga aaaatttcag atcttatgag
     3661 gaagaaacca ccttcaatct aaatactact agtgaaaaaa atattatatt gataggtggt
     3721 aaaaatggtg ctggtaaatc cacaatattt gaggctataa agctatgtat atatggacct
     3781 atagcttata aatatcaagg tttcaactct tcatatatat ctcgagttaa atctaatata
     3841 aataataatt ctctaagaaa tagtaaggtt gacaccttag tatcagtaga tatagaaatc
     3901 agtgaaggta ctgaaaaaaa tacctatacc ttaattagac aatggacttt taaagataaa
     3961 aaattaaatg aaacattttc agtttataaa aatttttcat ttacaccatt aaatgctgat
     4021 gaacttaatt attttgagaa ttatattaca tctattattt ctcctaaaat atttgacttt
     4081 ttcttttttg atggagaaca tttgtatgat ttttttatag gtaaaaattc aaatatacat
     4141 ctaaaagaat ctttattatc tctttgtaat tttgatacat ttgatatttt aaaaaaaatg
     4201 ttaatttcaa ctaacagatc caataagaat gtttcagatg agattaatat tataaaagat
     4261 gactatttaa atttagaagt tgaaatcaac aacttaaact ctcaagaaaa aaatctatct
     4321 tatgaattag aaaaaatttc aagtgaaatt gagaagttac aacaagaaca attaaaaata
     4381 aagaaagaat tcaggaaaaa aggtggaatt ttagcagaag aaagagaaat gcttaattct
     4441 agaattgctg aattagaagg aaatagattt ataattaatc aaaatataaa agatttttgt
     4501 aatgacatac ttcccttttt aattgtaaaa aaacaaatat taaacttaga aaaacaactt
     4561 aaagtagaga atgatagtat attatattct catcttaaag ataaattaaa tattgagtat
     4621 ataaaaaatc tactttcaga taagatcatg tcttcctcat tagatgaaat agctctaact
     4681 gtttctgagg ctttaataga agatataaaa ccagatagtt atgaagataa ctttgaatct
     4741 atccataagc tttctaatga tgaaagtagt aaattattat ctttgataaa taccatttta
     4801 aattttgata ataattctat cattaattcc tttggagaac atcgaagtat ttcaaatgaa
     4861 ttggcagaca taagacataa gttaaatact agctttaaag atgaaacttt gaatacatat
     4921 ataaatgata tatctagttt atcagataaa attgattctt tattaaaatc aaagaatttg
     4981 ctagttactt ctttagataa attaagactt gaaatagctg aaactatcac taaaagagac
     5041 aaatctaagg ctaaatatat atatttactt caacataata atattgttga tatatcttct
     5101 aatcttatac ttatgttaga tgaaataata acaacattaa cagaaactaa aattaaacta
     5161 gttcaagata attttatgta tatatttaaa aggattattc aaaaagacaa ttttattagt
     5221 tctatagata ttgatgccaa ctttaatatt tctttatata taaataaaat atataactct
     5281 ttagaacttg aaaatttagt agaaaatata ggatctgaag aaatggagaa gaagttggga
     5341 aaattatttt ttgaggattt atttaactta tatcgtgtaa aagataaaga tgatctattc
     5401 aatgctataa aaaataatac tcaatctaca tttttaaatc taagaactaa agttgacata
     5461 gacgaattct ctagtggtga aaaacaaata tacattcttt gtttatattg ggctttactt
     5521 aaatcttcag agatagaaat accatttatt atagatacac cttatgctag aatagatgat
     5581 actcatagaa ataatattac tagtgaatat ttttctacaa taagtgatca agctataata
     5641 ctatctacta atactgaaat agatgagaaa tcatataaga taataaagcc taagttaagt
     5701 aatgaatact tgattgaata tgataataaa aataaaaaaa ctatacaaaa taaaggatac
     5761 tttttcgagg tgtaattatg ggatttagac ttaaaacatc catacaaaca aaagaaatat
     5821 ttgaagaatt aggatctaga atgaatttga aaccatttgc tctttgcaaa ctagctatct
     5881 caatctcact aagtaatagt gagccaattg aaaactatga aagcatcgat aataatggtc
     5941 ttgaacttaa tagacaaact ataaccaatc aatatgatac tctatttaaa actttacttg
     6001 aacagcatgc aggtagacat ttaagtgatg atgaatactt tcctaaccaa gttaagcttc
     6061 atatagatag aggtgctctt atgcttaaaa acagatataa ttattcaagc aacttagaac
     6121 actttatcac tcaaatagta caggaaaata ataatctata aattaattaa tatttattaa
     6181 acataaataa attaaaacat gggatagaca ataaataaaa ttgcctatcc catgttttaa
     6241 ttataagtat tttttctaaa ttcatttatt ttatttatta agttcccaaa atgctttatc
     6301 tctaatatat ataggtgagt tctcactagg cattttgact aagctttcat gtttagctag
     6361 attctttatc ttattatata tgttatcttc ttcactttta ttcttagaat tgaatatgta
     6421 tcttatgtct ttattattta tgtctgatac attatgtatt atccaagttg aaatataatt
     6481 ctgatactca ttttctgaag ttgaaaaatg acttagaagt tgtgttgata gaatagttcc
     6541 tactccaaac tctctacctt ccttcaatat attttttata gatgtaaagt ttctactcaa
     6601 gaaattatca gcttcgtcta ctaatatcat tttatttaat tgtctatact ctccatttcc
     6661 agttatatca cttgatccaa gtgccttcat ttgactatag aataaatcaa gagttatcgc
     6721 aactactaaa ttttgtattc ctgaatcata atatgataaa tttataactg taactccatt
     6781 tattaaatca aataaatttt tagtcttctc actatttggc tcaaatattt caaaatcatt
     6841 aaaacttgaa agagcagcat acaatttatc gtcttttttt atttctcgat catcaaggta
     6901 tatcgataat acatcttgca ttgtagggca tgattttacc catgtatttt catcatcttt
     6961 ttttattcct tttttttcat aagctttcat aattaattct ctcaaaataa cttcttgctt
     7021 agggcctaag ccaaatgctt ttgctattgt ctcttttaaa gaattcgctg tgtgaacagg
     7081 tagtagtggc tttattcttt caggtattat taaagttaat ggattataag gtaaatgata
     7141 tatttcatat accttagcac ttgtcgcatc tacaaaatca tcttttatgt agtcgccttt
     7201 ataatcaaaa atcagtattc ctattggttt accatttatg ttatgttttt gttctctatg
     7261 aatttgcgtt actaaagatt tagtaaactg tgttttccca gttcccattg ttcctattat
     7321 accagtatta ggatgaggca ctttaatcga atttgttgga tcccaaataa gttctttatt
     7381 attatttttt atatttccaa atataatttt cattaaacca cttcaatcat aatcttttgt
     7441 tttatttagc ataacattag tatctaaaat aacattctta tccatatttt gtatacctat
     7501 tgtattatct aaatacttat ttataagcat ttcttgtata acaaaatcac tctctcctct
     7561 aatatacttc tccttcattt cctctaaatc catcactata ttattatatc cttcttgttc
     7621 tgaaagattt agtatagtta tattgccttc tttgcctaaa ttcccattat aataagttcc
     7681 ctttttaaat gatattacag ctcctcttcc tataaattca tctaaatcat tacttatttc
     7741 ataattatca ttcaacaagt ttgttcttat atcactattt attatatcat cccaattttg
     7801 ttcatcccat atatgatata acttcatttt ttgtgcactt actatagcga gttgtatcat
     7861 aaaattcctg tacataagct ttgtaaactt ttgggaatca ttttcgcttt cagtcaagta
     7921 ctcttctatt aaatctcttg tcttattagc ctgaattatt gcctttttta tgacactttc
     7981 attattatct ccaattttca cttcaacagg ataaaaatat acttttattt tattattttt
     8041 attttctatc ccaacaagta ataaatcatc tgaataagat ccagtatctc ctgttagttt
     8101 cttaacagta aacaaaccac ctgcttgttt taatccagct ccaccagata ctctaagtat
     8161 ttcttcaaga gatattggta tccatattat atttggatga taaaaataag ctaagcttat
     8221 ttttatagct gataatatgc ttaatttttc tctaggaaac tgagatttac tagatataag
     8281 ccttaataac caatctccat ttatagcatt gaaataatta atcaaattag ggattgtgtc
     8341 ttcttccact ttaatatctt tactatttag aaattcttca attataaatt tatattgttc
     8401 agattttcta gtaactgtta tagcatcata tccactagat gttgtatatt gatcactata
     8461 atgtattatt aataagtcat ttgaattctc atcatcctta aaaaaattta aatccacttt
     8521 tggatctata aaagtaaccc aatgagatgt cttataaatt ttatttaaaa atgaactatt
     8581 gttttcactt attgatgttg tcacactaag attatcatta aatggatctc cagttcctat
     8641 tgattttgta agtgaattaa acttcattga aatatttaac agttcatttt tagttgtagc
     8701 cataaactta gatccaaatc cagtcctata agtatcctct aaatatattg atggaactcc
     8761 tgctatcaat ccatttaatg ctacacctgt ttttatatca tccatagagc tacttgttat
     8821 atttacatcc ttatccattt gatataaagt caaatgggaa tactcatata aatctgacat
     8881 tttatctttt ttataaaatt taactttatc tctaaataaa tttaatacat cttcttcaga
     8941 gtaattgtct acacttaaat ttacattaaa atgttttttt atatcattta ttgaattata
     9001 aaatgctatt tcttcaaatg aatttataat acttttatct ccatatatat aaatatctat
     9061 tgatattaag ttatttattt cctcaccttt acctaatttc tttatgtaat aatcaaatat
     9121 accaagtaat acttctttac aatcacctaa atttattagc tttatcttca ttactgattt
     9181 acttatatcc ctaaataaat atgtaaaatg ttctataaat tcatttattt tttcctttgt
     9241 caatttagag acataatctc tagatccctt atatctactc atgtcatgat caacatagta
     9301 tgtccactct ggtgaatgtc tttgatctac taccttatat aaactattct tacctttata
     9361 tatatagggc attagatact tcgaacccaa tagttttaag attccttctt taacttcttc
     9421 atcacctata acattattta tcattaattg gtaagccaca tttactggat gtaatggtgt
     9481 taaaaataat tcttcttctt tatctattct atgtatagtc cctaccttag ctatattaag
     9541 tctctcttta gataatactt ttccattttt tatatcttca aacacttcta atatagtatt
     9601 tatataattt tttgatatgt taactaaatc cacatcataa tatgccaagc ttggaataca
     9661 atcatttact ttatagtaac ttataaggtt tttgtatgct tcttttattc tggaatctac
     9721 atccaattct attattttat atttttcttc taaaatagta caatgcatta tattattttc
     9781 tatgatatat ttttctattt gcaaattttc tttaaaattt tcttttggaa aatattcact
     9841 agtaccttgt acaattttac tattacctaa atatttaaaa ctctcctttc gttctctctt
     9901 taatttccag gttttcattc cacttataga aattggttgt tctttttctt cttgtttaac
     9961 tatggtaatt acagattgat catatactac atcaattttt ataaattcaa catcttcttc
    10021 caattcaaca ttaagttcta aatctagcct ttctccttct tcaagattaa tttcattttc
    10081 aaactcatct atttgtattt ttctctcaat tatacctttt gggtttatta ctattgtatc
    10141 atcatctgtg ttagtaacta acatacttct ttttgaatta ataacaattc cataattact
    10201 ttttatatca tttaatatat tctcatcaca agatactata caaattctaa attcatattt
    10261 tattatacca tcattatatt taactttagt aaatgtagtt tctgaaatat ttatatttat
    10321 acttatataa gctttatttc cacttgcttt tacttctgta tatgttttag acatcttatc
    10381 tatagaaaaa cttgtttttt caattttctt atcaaatttt atgcataaat ttataatatc
    10441 aagtttgtct ttattaaata ataatatgtt ttttcgtttt gacttatttt caatatctgg
    10501 tttactccag agctttaggt tttcaacaca ttctatacat aataactcta cctttttgtt
    10561 tttcattctt tcttgagatg actttatatc tttataggta gaatacttcc aattctcatc
    10621 ttttaaaaga tctatacctt ttgaatcaaa atgtttctct aattccagct tagggtctcc
    10681 atactgatgt atagagttta caatagaaaa cagcttttca ttatctaata gtctttcttt
    10741 tattgccttg ctacttttgt tgtctaacat attatcatca taaaataatc caaatttact
    10801 atattcactc ttttcaattc ttcctttatt taatatatct aatatatttt catattgaaa
    10861 tattgatgaa ttatcttcta atatactttt attcattcta tcaagtacaa aataaagtat
    10921 atgtttaata tcttctttta atttatcatt ttctaattta ttttgtatgt cctttactaa
    10981 agtttttgta tgaaatggca taccttcttt ttgcaaactt tctgttcctc ttattaaact
    11041 atctaatgtc atattatgta taaataatat ggctgaatcc ttaaattcat cactagctac
    11101 atttcctata agatttctaa gtctaattaa aaaatcattg gttgtattat tatttgaagc
    11161 aactattaat tgaataccac tgacattcat actaaaagtc tgatattttg atccattcat
    11221 tatattatat gtaaatggtc tactattact ttctttctct aaaacatcat acataatttt
    11281 aacttgttct tctctttcaa actgtatatt aaatttatcg ccacttttta tttctctaga
    11341 ttcgaaatat ttaactagat ttttagctag atatctataa aattgatctg acatattgtg
    11401 catccccact atcactcttt ttttctatta tatttaattt ttcaaataac tctactactt
    11461 tgctttgaga atctctatca aaataaattc ctcttttttc atattcttca aataattcct
    11521 ttaattttat ctttgtctta tttttcatac ataattttgt cataaataac agttcttctt
    11581 gattaatttt caatacattt cccaatggcc ctgctttttt taaaaaattt aatttacaat
    11641 actcttcaaa taaattttta tacctcttat agttagcctc tcttgaagat ttcttgaact
    11701 gataatctat tcttttatgt aaatcacata tttcttcttt tatgttatca ttcttataca
    11761 ctttagtttg ttcatcataa tcatcccatt gtacatcagt aattgacttg acataagtat
    11821 tttttatatc tattatattt tcatataacc taagtctttc attttctgat aaatttttta
    11881 taattttatt taaatctaca taatcatact tactaatgtc attattagta tttaatatct
    11941 ctaatgtatg aacatgacta aataatattc taatatgact ctccaacatt ttccaaccca
    12001 tgttataact tattcttgtt ttagatgctt tttcccaact taaaataaaa tatacttttt
    12061 cagttctatc tctatctcca tcaaaaaagt gagataattt tattgataat tgtgatacat
    12121 agaaaaaata atagtacttt aataatcttt caaactcgtt tataattaat tctggataat
    12181 taagcataaa ttctaaatct tcttggaata atttagacac atttttacat atacatttat
    12241 a
//
LOCUS       DAELNY010000114        12106 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.114, whole genome shotgun sequence.
ACCESSION   DAELNY010000114 DAELNY010000000
VERSION     DAELNY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.114"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            146..964
                     /gene="larE"
                     /locus_tag="KPE55_002550"
     CDS             146..964
                     /gene="larE"
                     /locus_tag="KPE55_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG0493604.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1019..2167
                     /locus_tag="KPE55_002551"
     CDS             1019..2167
                     /locus_tag="KPE55_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG0493605.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            2303..3691
                     /locus_tag="KPE55_002552"
     CDS             2303..3691
                     /locus_tag="KPE55_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0493606.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            3720..4418
                     /locus_tag="KPE55_002553"
     CDS             3720..4418
                     /locus_tag="KPE55_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493607.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            4421..5854
                     /locus_tag="KPE55_002554"
     CDS             4421..5854
                     /locus_tag="KPE55_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493608.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            5876..6613
                     /gene="truA"
                     /locus_tag="KPE55_002555"
     CDS             5876..6613
                     /gene="truA"
                     /locus_tag="KPE55_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0493609.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    6724..6931
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7184..8383
                     /locus_tag="KPE55_002556"
     CDS             7184..8383
                     /locus_tag="KPE55_002556"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG0493610.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            8630..9676
                     /locus_tag="KPE55_002557"
     CDS             8630..9676
                     /locus_tag="KPE55_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG0493611.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            9784..10947
                     /locus_tag="KPE55_002558"
     CDS             9784..10947
                     /locus_tag="KPE55_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0493612.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(11036..11416)
                     /locus_tag="KPE55_002559"
     CDS             complement(11036..11416)
                     /locus_tag="KPE55_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG0493613.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(11419..12078)
                     /locus_tag="KPE55_002560"
     CDS             complement(11419..12078)
                     /locus_tag="KPE55_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG0493614.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         4876 a         1212 c         2109 g         3909 t
ORIGIN      
        1 ggttaaaaaa atactttaat atttttaata ttataattaa tagatttaag gtaattataa
       61 tttataaaat aaatacttta tatgttataa tcattaagaa tgtaaaaaaa taatatatag
      121 ttttatagtt catatttttc cagatatgga ggtaaatttt gtgaaggaaa aagaaaaatt
      181 agataaatta aaaaaaatgt tacttgaatt aggcagtgtt gtagttgctt attctggtgg
      241 agtagatagt aattttttgc ttaaggtcgc aaaagataca ttaggtgaaa atgttatagc
      301 tgtgactatt catgcaatga tgcattcaag tagggaaatt gaagaggcta aacaatatac
      361 tcaaaatttt ggtgttaaac atatcatatt aaatatagaa aactttgatt taaaagaatt
      421 taaagaaaat ggtgttgata gatgttatca ttgtaaaaaa tatatattct ctaaaataaa
      481 agaggtagca aaagaacata atataaagta catagtagat ggaacaaata tagatgattt
      541 aggagactat agacctgggc ttaaggcatt gagtgaatta ggagtaatta gtccattaaa
      601 agatagtagt ttaaaaaaag aagaaattag gtcgctttca aaaacattag gattaaatac
      661 ttttaataaa ccatcatttg catgtcttgc gagtaggata ccttatggag ttgaaataac
      721 agatgaaaat ttaagaatag tagaaaagag tgaagaatat ctatccaatt taggtttttc
      781 tcaatttaga gtgagaatgc atggagatat agctagaata gaagttggac aggaagagct
      841 aggtaaattt tttgaaaata acaattttaa taaagttgat actaaattaa aaatatttgg
      901 ctttaaatat gttacactag atatgtcagg atataaaatg ggaagcatga atttaaatgt
      961 gtagattatt ttaaaaataa ataagtttta tagttaatat aaggaaacag gaggttttat
     1021 gaaaaattat acgttaatat cgccatgttt ttttggaatg gaaaaaatgc tggctagaga
     1081 gattactaat ttaggatatg aaataataaa aacagaagat ggaagaataa catataaaac
     1141 tgatgagttt ggaatagcaa agtcaaacat gtggttgaga tgtgctgaaa gagtacatct
     1201 taaaattgct gaatttgagg caaaaagctt tgatgaatta tttgaaaata caaaaagaat
     1261 aaactggtct agatatatac cttatggagc tcaatttcct atatcaaaag cttcctctat
     1321 aaaatcaaaa ttatacagta caccagatgt acaagccata gtaaaaaagg ctatagtaga
     1381 aagcttaaag aaaagttatt tggaggatgg tttgcttaaa gaagataagg agaaataccc
     1441 tatttttgta tttatacaca aagataaagt aaccatttct atagatacaa caggagatgc
     1501 tttgcataag agaggatata gagaaaaggc aaataaagct ccaataagag agactttagc
     1561 tgctgggctt atttatttaa ctccctggaa agcaggtaga gttttagtag accctatgtg
     1621 tggttctggt actatattga ttgaagctgc tatgattggt ataaatatgg cacctggctt
     1681 aaatagagag tttatatcag aaaagtggag aactcttgat aagaaaatat ggtgggatgt
     1741 aagaaaagat gcttttaata agatagataa tgaatctaaa tttaagattt atggatatga
     1801 tatagatgaa gaatctatag atatagctag agaaaatgct gaaatagcag gtgtagatga
     1861 atatatagag tttaatgtag gagatgcaac tcaatttaaa agtgaagatg agtttggatt
     1921 tataatcact aatcctcctt acggagaaag attagaagat aaagacagtg ttaaacagtt
     1981 gtataaggag cttggttatg catttagaaa attaaaaaat tggtcgtatt atttgataac
     2041 ttcttatgaa gactttgaat atgagtttgg gcaaaaagca gataaaaaaa gaaaattgta
     2101 taatggtatg ttaaaaacta actttttcca atatccgggt cctaagcctc ctagaaataa
     2161 taaataagtt ttacatgatt tatttgtaaa atataaaatt ttattaaaat aagtaaaaca
     2221 ttatgatgcc ctgaaacgtc taattattgt gaggagattt ttgtgaacaa atttaaagat
     2281 ttataggatt aggaggagtt aaatgaaagt gttaaagctt attactgtag tagccctgat
     2341 gatgttattt gttactggat gtaatataaa aactgaatct ccagaacaat tagcgaagat
     2401 acctgactat gatgatacaa aaaaagtatt gtatgatggt attgaccagt tactaaaacc
     2461 agattcatct atgatattgc cttctaatac aaaagaagta ggaaaaataa ataaggtaga
     2521 tttaaacagc gatggaattg atgaattagt agtatttgaa caaaaagaag atttaagtaa
     2581 taatgtgagt caggtagggt ttgtaacctt gagttacaat ggagaaaaat acgtacttgg
     2641 agaccatttt ttagaaaatg gcgaatcaat agaatatgct aatttttatg atttggactc
     2701 agatggatat aaggaagtta tcttattggt taaatccaaa gacaaaacga atatgcatat
     2761 atataaagta gtagataatg aaataacaaa aatatatgac ttggatgcat catggcttca
     2821 aaataaagaa gattttaatg acatgaaagt aaaaattgga tatatagatg gagatgacaa
     2881 gttagacata ttgatgcttc atttgaataa taagactaat gaaatgtttg caagtgttgc
     2941 aaattttgat gggaaaatga aaatcaaaga ttctgttaag tttgaaaatg taaaaaactt
     3001 aagtgaatta tatataacat taggtaatgt tgcttcatct gttggaaatt cttctgcagc
     3061 tataaaaggt attgtacttg atattcctat attgaaagat aataactata ttacgcagat
     3121 actttatatg aaagatggga aaattaacaa agcttttagt gattatgata aaactattac
     3181 taaatcttat tatatacctg tagatgatat tgacaaagat aagattatag aaatacctat
     3241 agttgcaggc agtacaggta ataataaaaa tacttatagt tctaaatcat ctgcaactat
     3301 aagttggtat agatggaatg gaaaagaagg tgctgactca agtttaatat ttacaagtca
     3361 gatatattat aattataaat ataattttaa attatttatt ccaaataatt tatttgataa
     3421 aatacttgtt gaacaggaat ttgttggaga aaaagcagta tttaaatttt actattttga
     3481 ctattcagac cgtaagaatt tatttaatat agtagtagag agtaaaaata aacttgaaga
     3541 tagaaaaaat acaggtactc aaaattctat tgtacttcaa gagagcgatg aatatacttt
     3601 cttactagtt gtaaacaatg caaatgaaat ggataagcta gatatgacta tagacgcatt
     3661 aaaagaatat ttctcattaa tatatggtta atattatact caattggagg ttttatttta
     3721 tgaaggaaaa gatacttata ctagaagatg aaattggtat tagaagtttt gttagtataa
     3781 acttaaaaag ggaaggctat gaaatagttg aggcaggtac tggaagagaa gctatagaaa
     3841 aaatgacaac agaaaaggat attacaatag ctcttttaga tgtaatgctt ccagatataa
     3901 gtggtataga agtatgtaaa tttataaggg agaattttga ccaagtaggt ataataatgc
     3961 ttactgctaa agctcaagaa gatgataaaa tagaaggatt tatctcagga gcagatgatt
     4021 acatcataaa accatttagt ataaaagagc ttttagtcag agtttctgct cttcttagaa
     4081 gagtagcaaa ggatgattca agtgttaagt cgagtgagat agtttctcca ccatttatac
     4141 ttgatataga caaaagaaaa ttatttaaaa atggaaagga aattgagctt acaccaactg
     4201 aattttcaat agtgaaatat ctaatatcaa atgctaagca gtcgttaagt agagaccaga
     4261 tattagacga agtatgggga actaactatt tatatgattt taaaatagta gatgtaaata
     4321 taagaaggat aagaaataaa atagaagatg acccatcaaa accaaaatat atacaaacta
     4381 tttggggtta tggatactgt tttagaaagg aagaataata gtgttacatt ttgaaggtct
     4441 gagaaaaaaa gtaataaaaa actattttat aataattatt ataatggtta ctctgtttga
     4501 aggattgttc atgttttaca tacaaaacta ctattatgat tctgtcaaac agctacttga
     4561 atctgaaata aaatatgctg atgaatataa tgctattaca atggaaacta caagttttga
     4621 gaaaaaagta aaaaatatat ttgataaaca acccttaact aaaaattcag aatttggaat
     4681 ttcaataata gataaagata aaaatattat attagaccaa tatggtttta aaagtaaaga
     4741 aaaagcaaac tatgaagatg ttaacaatgc attaaaagat ataaaaacta aaaatttaac
     4801 accttatact tatagaatac cagatacagg ggagcatgtt atgagtatct ccttaccact
     4861 taaggtaaat aatataatag aaggtgttgt tagatataca gtatcattag atgctattga
     4921 taatgcaata ttaaagcaag ctacatggct tatattagca ggaatattta tacttattat
     4981 agctatacta ataagtctta aatttgctga gactcttata aaaccattac gagagctaaa
     5041 gaaatttgca aatgaattgg ctgtaggaaa ttataatata aaattagaaa agatgaagat
     5101 tgtagatgat gaaattggtg atttagcaca gacatttgaa cacatggcac atgagattga
     5161 caaaagtgaa aaattaaaag aggaatttat atcatctgta tctcatgaac taaggactcc
     5221 tcttacatct ataaaggggt ggagtgaaac cttaggttat gaaagtataa caagagaaga
     5281 actagactta ggtttaggca ttatacaaga tgaaacagaa agacttataa aactagttga
     5341 agaattatta gacttttcaa ggctttcttc agacaggata aaacttcatg ttgatatagt
     5401 tgatgttgaa ggtttaattg taggtgttgt aaatcaactt aaagttaaag cagctgaaaa
     5461 agatatatca cttttatttg aatttgaaaa tgaatttata gagaacattc aaggagataa
     5521 aaatagatta agacaggttc tcataaattt aatacaaaat tcatttaaat ttacgagtca
     5581 aggtggttac ataaaagttg tagcttctca agatgaagag ataacaacca tatcagtaga
     5641 ggataatggt tcaggtatag aaaagcaaaa tttaaataaa gtgttagata aatttttcca
     5701 agaagattat aataaggctg gtagtggatt aggactagca attagtaatg aaattgtaaa
     5761 acttcatggt ggaagaatga aaatagaaag tgaaaagaat gttggaacaa aaataacttt
     5821 taatataaaa aataaatttg ctaaacaagc ataaattctt agaaagagga ttaagatgag
     5881 aaacataaaa atgatagtag catacgatgg ttcaagatat aaaggatatc aaaaattagg
     5941 tgacaataat atgactattc aagaaaaact tgaaaatgtt ttaagtaaaa tgactaatga
     6001 aacagtggag ataataggtt ctggaagaac tgatatggga gctcatgcaa gaggacaagt
     6061 ggtaaatttt agaactaatt gtatggattc tttggataaa attcaaaaat atttatatga
     6121 atatttacca gaagatatag ttgtaaaaac tgttgaagaa gttgatgaaa gatttcattc
     6181 aagatacaat gtaaaatcaa agacttatat gtataaaatt gacaataata agtatcataa
     6241 tccttttata agaaaatatg ctactcatgt aagtaaaaaa ttagatttag atagaatgag
     6301 aaaagcaagt gagtatttag taggagaaca tgattttact agttttgctt cttctaaatc
     6361 taagaaaaaa tcaaatgtaa gagaaattta ttctataaat ataaaagaag atgataatgt
     6421 tatagaaata tatgtagaag gaaatggatt tttgtataac atggtaagga taatagtagg
     6481 tgctttgata gatgttggtc taaaaagaag agctccacaa gatattaagt atatgcttga
     6541 atcaaaagat agatgtcaat catcagatac tgcaccagct aaaggactat gtctttggaa
     6601 agtaacatat taatcaatta tataaaactc tctttaaaat aaaaataaaa taaattatat
     6661 agttgacata aatggaaaaa taaactatta tatattttat aagagagata taaaacttta
     6721 tatttaaatc ctgtgagaaa gagagtaact tctatgtgtt ttatagagag ctgaggatgg
     6781 tggaagcttg gcaaataagt attagtgaaa agaactttgg aggatactaa ttgaatttag
     6841 tatgttagta ggtaatctcg ccttaagaga aagagtggat aagtttatct tatcaattag
     6901 agtggtaacg cggatatatt tcgtctcttg gtttttccaa gaggcgtttt ttaatatatt
     6961 taattattag tgtttatctg gtatgttgaa catttactaa ttgattagct aagtttatca
     7021 ataagagtgg taccgcggat atttcgcctc ttagtatttt aaaatactaa ggggtttttt
     7081 tagtattaaa atgattttat gtgagtctaa attctcagag tcattcaata ttaagaattt
     7141 ctataaaata aaaattataa tttaagattg ggaggaatta aaaatgaagg aaaaagtagt
     7201 attagcatat tcaggaggac ttgacacatc aatcataata ccttggttaa aggaaaatta
     7261 tgaagatata gatgttatag ctgtttgtgg aaatgtaggt caggaagata aaatggaaga
     7321 tgtatatgaa aaagctttac aaagtggagc atcaaaggca tatgtggatg atataagtga
     7381 agaatttgta actgaaacta tatttaaagc cgtaaaagca gaggctaaat atgaaggaaa
     7441 atacttatta ggaacttctt tagcaagacc tataatagct aaaaaattag ttgaagttgc
     7501 tcataaagaa ggtgcaaaat atatatgcca tggatgtaca ggaaaaggta atgaccaagt
     7561 tagatttgaa gctacaattg cagctcttga cccaactata aaagttattg caccttggag
     7621 aatttgggat ataaaatcaa gagaagatgc tatagactat gctgaaaaac ataatataaa
     7681 ggtaacagca actaaagcta aaatatattc agtagatgcc aacctttggc atgtttcaac
     7741 agaaggtgga gatatagagc acctagaaaa tgaacataaa aaagatgttt ataaacaatg
     7801 tgtagaccca gaagatgcat gtgatgtagc tgaatatgta gaagtttact ttgaaaaagg
     7861 tgtacctaaa aaggtaaatg gcgaagaatt atctccagtt gcactgattc ataagttaaa
     7921 tgagttgggt tgtaaacatg gaattggtgt tatagatata gttgagaata gacttgttgg
     7981 tatgaaatca agaggtatat atgagactcc aggaggaaca atcctttatg aagcacataa
     8041 tattttagaa agtgctactt tagacaaaga tacattacat ttcaaacaaa tggtatctta
     8101 taaatacgga gaacttattt acaatggact atggtattgt aaattaagag aatctataga
     8161 tgcatttatg gaacaaactc aagataatgt tacaggaaca gtaaaagtta aactttacaa
     8221 aggaaatata aaacctgctg gaatatttac tgaaaatgct ctttatgatg aaggaatatc
     8281 ttcttttgga aatagtgaac tttatgacca caaagatgca gaaggattta ttaatttatt
     8341 cactttacca ttaaaaatca gagctatgaa agctggaaaa taataaaata aattatttaa
     8401 gagtgtataa aaacgatttt taggttttta tatgctcttt ttttatttga atttattaaa
     8461 ttatacagta aatagtataa aaaaatatat tatagttcca aatggcttaa taatgtgaaa
     8521 atataagacc taaaatataa attttataaa tacaatagtt attgactctc tcattgtaag
     8581 agggtttata ctgaatctaa tgaaaattta tagtgaggag atagattata tgagaaaaat
     8641 aattgaaatt aaacatcatt cacatgatta tgagtgtatg tggaatggta tagaagattt
     8701 atatataaat aaaaccggtg aataccttcc aaacgatttt ttctttttac tgtcaggttt
     8761 tggttcattt tgttatatga aaacaaataa agctgatttg aaaagaatgg tagctttggg
     8821 agatggaagg acaaaacaga tgtataaatt tttagcacca attgttggat ttgaatacaa
     8881 acatcatgag tttaaaaagt atgagcaagc attaaagaaa gcaaaatctg aaattgatga
     8941 tggatatcca gttattttag gagctttaga tatgtattat ctaccatatt ataagaagct
     9001 atatcataaa gaacatattc cttttcatta tatattaatg gtaggatatg atgatgaagg
     9061 aatatatctg tatgattgtg gtagaacaga attattacat ctttcttatg ataaattacg
     9121 tgaaagcatg aattgcaatt cccctggatt aagcaaagaa aatactattt gcacaataag
     9181 aatgaatact tcaaagaata aatataacat tgctagagaa gctattgcta taaagcgtga
     9241 tatgttttta aatccaccta aaggattttt gggatataaa ggttttgaaa agtttattaa
     9301 agagttgcca cagtggaaca ctgaattgga taaggatgat tatgataaga ttttgaccaa
     9361 tatggttatg ttttttggaa cagtaccaac aattccaaat gcaatcaaag gaattaaaga
     9421 accagatact gttcaattta aaggtggatt tgataaaatg agtagaatgc tcaacagtat
     9481 tggggaggaa tacaaaaaca actcttttat acaagtagct gatatttttg ataaaactgc
     9541 tattataatt gagaaaatat ccaatattat aatagattac ttaactcaaa catgtgatga
     9601 tacagaacaa cttccgttat tgttctctga ggtcttagag catatgaaaa caggatattt
     9661 aatcttggat gtataaaaaa tatattgttg accctaacat tacgtaatag tgtaatatga
     9721 aaatatagct taaatatatt agtaggtatt gatggctgta aaattaaaat ttgaggtgtg
     9781 gatatgagaa cagtgaaaca agtttctgat ttaactggaa taagtgtgag ggcattacat
     9841 tattatgatg aaataggtct tttaaaacca aataaaatta cagatgcagg atatagactt
     9901 tatgatgatg aatctattaa aactcttcaa caaattttat tttttaagga gattgatatt
     9961 cctttaagag aggttaagga aataatgtca agccaatatt ttgataaggt tgaagctcta
    10021 aaaaatcaaa aaaaattact tattttaaaa cgaaagagac tggatgaatt gatagagctt
    10081 ataaatcaaa ctctaagagg agaaggaaat attgatttta aagagtttga tatgagcgag
    10141 tattttaatg ttttagaaga atttaaaaga gagcatagga ataaggtaat taaaatttat
    10201 ggtagtgtag aaaaatacaa tgaatatata gaaagagtta agtctaatga agagaaaatt
    10261 gctaaaatgg ctataaaaca atatggaact attgagaagt ttgctaaagc tataaaaaca
    10321 aattttagca gtgatatatt gaatttagga gaaaaatttg atagatataa aaatgattgt
    10381 ttaaaagaaa aacatccaaa attaaaagaa ttatatagaa agcttgtaga agatttaagt
    10441 aggaatcctt cttcaacaga ccttcaagaa atagcaaaag cgataacaga tatatctaaa
    10501 aaagattatg aaattttctc tatggatact ggtgatgaca actggtatta tatggttcaa
    10561 aattatctag taaatcctat gtggatagaa gaagtagata aaaagtatgg aagtggagca
    10621 ggaaagttta ttggacaagt actaaagact tatctaagag atagaaagcc aaagataaat
    10681 acattgtatg aaaaacttgt agaagatttg agtagagatt gttcttcaag ggaagttcaa
    10741 agcattgttg aagaaataga caatgaaatg aaaagaagta atgaatttta taaaatagac
    10801 aatggtgaaa gatactttga ttatatgtca gagctttatt tacaagattc taactatata
    10861 aaagtaactg ataagaatta tggtgatggt gcatctaagt ttattggaga agcttttaag
    10921 atttattttg ataataataa ttgttgaaaa tttggaagta aaaaatatgt aagttttata
    10981 aaagcacaaa aataaggatg tagattagct ccatctatat ccttatttaa agttattaaa
    11041 atatatagtt tacatcaaac ttaacacttt caataatatt tcccaaatat gtttttatac
    11101 tttcaacttc ttctaaagat tttaatttat tttcagtata aattgctttg tccagaatat
    11161 taaaccttaa tcctatttca tcaaagtact catgatttaa aactttgtac caatggtttt
    11221 tttctttatt ccaactcgta gatattgaat cagcttcttt ttgagctttt tcccaatctt
    11281 cattttctat agatttttct aaaacagata tattgtcttt atatccttct gtaaaatcat
    11341 atagtttatt gttaacgtaa aatccaaata atacaaataa gatagtccat agtataacaa
    11401 atgttgtaga tttcaatatt aatcaccctt tttattttga ataaatattt tgtcattttc
    11461 atctaaaaca caaataagaa catctttaaa agtttctatg tgattagatt ttaaaatacc
    11521 cattaaccag ttagtatctt tttgagctat gtctagattt ttatttatta ttctaccatc
    11581 cagtataagt gggataggta aatgattaaa agctcttgga ggagtactat tgtagcttga
    11641 tgctggtaca acacttagat taccatcagt ctccaaaata gcatattgta catcttttag
    11701 gttaaagtat ccttgaatcc taagttgttc taataattca tctatactaa ttctttcttt
    11761 tttaagctct ttatatacaa tttttccttt atctattaat acagaaggtt tacctgataa
    11821 aaatgaactt aattttctgc tttttaatga taagaatgaa attagagtct gcatgattac
    11881 aagtccagat attgctgcaa caccatttat gattggtata ttattatttt ccattgggac
    11941 agatgcaact tctgcaatta atagtgttat aactaaatca aaacagttca tttcagctat
    12001 ttcacctttt cccatgactc taagtgcaat tagcactgtt atgtataata taatacttct
    12061 tattaatact actgtcatat ctatcaatta ctcctttaaa aaatat
//
LOCUS       DAELNY010000115        11821 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.115, whole genome shotgun sequence.
ACCESSION   DAELNY010000115 DAELNY010000000
VERSION     DAELNY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.115"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..753
                     /locus_tag="KPE55_002561"
     CDS             <1..753
                     /locus_tag="KPE55_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0493615.1"
                     /translation="ARCKNEEKIYLNIIKNHQYQCNNIINNNIEKFEENKEILNMWVH
                     DVKIPIAIIKLILEQNENLIDEKISDDIDSEIFRIENSVEKILYLSRLEDFHKDFLIQ
                     EVNIEKIIRNIIKKYSKYFISKKIILDLNNLNFKVLSDEKWLYFIFEQLLSNSLKYSS
                     LGDNISIYCKRTKNYLQLRVEDTGCGIKKEDLNRIFNKGFTGNNGRNNSKSTGLGLYL
                     VKELCDKLGHKIDVDSEYNQYTKFTITFKCDL"
     gene            865..2094
                     /locus_tag="KPE55_002562"
     CDS             865..2094
                     /locus_tag="KPE55_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0493616.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            2102..2776
                     /locus_tag="KPE55_002563"
     CDS             2102..2776
                     /locus_tag="KPE55_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493617.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            2779..4011
                     /locus_tag="KPE55_002564"
     CDS             2779..4011
                     /locus_tag="KPE55_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493618.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(4297..5562)
                     /locus_tag="KPE55_002565"
     CDS             complement(4297..5562)
                     /locus_tag="KPE55_002565"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG0493619.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(5615..6763)
                     /locus_tag="KPE55_002566"
     CDS             complement(5615..6763)
                     /locus_tag="KPE55_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0493620.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(6863..7408)
                     /locus_tag="KPE55_002567"
     CDS             complement(6863..7408)
                     /locus_tag="KPE55_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0493621.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(7631..8575)
                     /locus_tag="KPE55_002568"
     CDS             complement(7631..8575)
                     /locus_tag="KPE55_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0493622.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            8961..9842
                     /locus_tag="KPE55_002569"
     CDS             8961..9842
                     /locus_tag="KPE55_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0493623.1"
                     /translation="MVKKRKKKTVALTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(9903..11111)
                     /locus_tag="KPE55_002570"
     CDS             complement(9903..11111)
                     /locus_tag="KPE55_002570"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG0493624.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMDLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(11184..11436)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11588..>11821)
                     /locus_tag="KPE55_002571"
     CDS             complement(11588..>11821)
                     /locus_tag="KPE55_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG0493625.1"
                     /translation="FIIRDSCMSLQNFIIAKFRKLLYARILFLIIILLLFFIFDVTVI
                     GLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
BASE COUNT         4116 a         1912 c         1506 g         4287 t
ORIGIN      
        1 gctagatgta aaaatgaaga aaaaatatac ttgaacatta ttaaaaatca tcaatatcaa
       61 tgtaataaca taataaataa taatatcgag aaatttgaag aaaacaaaga gattttaaat
      121 atgtgggtac atgatgtaaa gatacctata gctataataa aactcattct tgaacaaaat
      181 gaaaatttaa ttgatgaaaa aatttcagat gacatagata gtgaaatatt tagaattgaa
      241 aactccgttg aaaaaatttt atatttatca agactcgaag attttcataa ggatttttta
      301 attcaagagg taaatattga aaaaatcatt agaaatatta taaaaaaata ctcaaaatat
      361 tttatttcaa aaaaaattat tttagactta aataatttga attttaaggt tttatctgat
      421 gaaaaatggc tatacttcat attcgagcaa ttattgagta attccctaaa atactcctca
      481 ttaggagata atatatctat ctattgcaaa agaaccaaaa attatttaca attaagagtg
      541 gaagatactg gttgtggtat aaaaaaggaa gatttaaatc gaatattcaa taagggattt
      601 acaggtaata atggaagaaa caattctaaa tctacaggtc ttgggcttta tttagtaaaa
      661 gaactttgtg ataagcttgg tcataaaatt gatgtagatt cagagtacaa ccaatataca
      721 aaatttacta ttacattcaa atgtgattta tagtatctta ttattgatag tttattttca
      781 ttaacttata ttcaacttct tatagtaatt ctatatgtac atcccagtat ataattaagt
      841 agtttttata aaaaggagga agacatgtct tttctaaaaa aatttaaaaa taaaagattt
      901 agaaataaca gttttaaaag taacaacttt agaaatagca gttttggaag tagtggcttc
      961 aagtctaaaa ataaaggttt taaaaacaat ccctttaaaa ataagagatt caaaatatat
     1021 gcaggaatag cagtaatcat acttttaatt ctaggagttc ttgcttatgt tgattcgaaa
     1081 aacactgaat tacaagctaa tgaaaatttt atcgaaacat atactattcc tgaaaatgaa
     1141 aaaatattta taaatgggat ggttgttcct aagcaaacta aggacttcaa tatatctgga
     1201 gattatgagt taagtgatgt taatgttaca aatggtcaaa aagtaaatca aggagacctt
     1261 ctttttactg ctaaaaatcc aactataata gcagaaatag atagtttaaa atcacaactt
     1321 agtcaataca aaaaacaaaa aatatcactt tctgatattg ctgaaaataa agatgctatt
     1381 gcttctataa atgcacagat aactgcttta aactctcaga tagcttctct ggataaaaaa
     1441 gcttatgaca gagttactgc tccttttgat ggcacagtat acttgaatga ccaaactgga
     1501 aatccagacc aaccagtttc atttatgact attcaaggtc tagagtttta tatgaaagga
     1561 caagcaagtg aacaagattt acctaaaatg aagattgacc aaatggtcaa tatactagtt
     1621 ttttcaactg accaaaagct tactggtaga ataagtttta tatctgataa accatctact
     1681 ccaaatactg aaatgggagc tcagcagaat acactatctt attatgatat aaacatagca
     1741 tttgataatc aagaaggatt agtaaatgga ttccatcttc aagcatcact tgaagtagct
     1801 aattcaagct ttaaaatacc agcatcttgt gtacttaaag acaacaaaca ctcatatgta
     1861 tttaaggatt tagatggtat attgaaaaag caaattgtag atgtagcaag tcaaaatgat
     1921 gattttgctg tagttagagg cggtcttgaa caaggcgata taataataaa acaccctact
     1981 aaagaaatga aagaaggaga cccagttcaa ggcgatggcg taactgctgg ttctaataat
     2041 ggagatacta ctccaaacaa aaaagtagaa gaagttacta tggatgtgaa ttaatttaca
     2101 tatgttaatt aaattagaaa atattcaaaa atattacaaa gtagggaaag atgaattaca
     2161 tgttttaaaa tccttaaatt tagaaattga atctggagaa tttgtcatga taatgggtaa
     2221 gtctggtagt ggtaaaacta ctttacttaa tatcttaggt tttctagatg tatttgatga
     2281 aggaagatat atatttgatg gaacagatgt aactaatttg agtgaaaatg aacgttctgt
     2341 ttttagaaat attaatattg gatttgtttt ccaacaattt aatttaatag aaactctaaa
     2401 tgtatatcaa aatgtagagt tgcctctaat ttacaataaa gcactaaaaa aatctaatag
     2461 agaagaaatt gttaaagata aattaagttc tgttgggctt ttggacaagc ttaagcagaa
     2521 accacttcaa ttatctggtg gtcaacaaca gcgtgtagcc attgcacgtt gcctagcaaa
     2581 tgaccctcag ataatctttg ctgatgaacc gactggggca ttagatagtg aaactagcag
     2641 agaaattatg gagcttttaa caaggctcaa caaacaaggt aagactataa taatggttac
     2701 acatgatcaa gatttaacta agtatgcaac taaggttata cgacttaaag atggtgtgtt
     2761 tacttcggag gtgtaatcat gagtttatta aaaaattctt tggccaattt aaaaggtcac
     2821 aagcttcgtg tatttgtagc tttactctgg attataatag gaataacttc agtaatactt
     2881 gttagttcta taggtaatgg atttcaaaaa gaaattaaga aatctgtaaa caatgtaaac
     2941 cctaataaaa ctactatttc gtttgaatca gccgataaca caggtcttac tgatgatatg
     3001 agtatttttt taaaaccctt taatgcaaaa gatttagaag aattatcatt tgttgaagga
     3061 gtagaaagaa tagctccttc aagagatggg tttaaccttg attcagttta ctcttcacaa
     3121 gcatcttttg ataaaaaaac tacgtatgtt gatgttggac cagtaaaaaa agattctaaa
     3181 ataaatttaa tttgtggtag agatttttct ttagatgatg aaaaaagaaa agtaatttta
     3241 cttacattac aaagtacaag tgaaattttt gaaaatccag aagatgcctt aggtcatggt
     3301 atcaacataa atggaactat atttgaaata ataggggtat tagatgattc tcagcaaaac
     3361 caagctggag gattctttgg aggttaccaa gatatgcaat ttactacttc cctagtacct
     3421 aaaaaggctt ttgacacttt aatgagtcaa aattcttatt caaatgaaat ttaccagtta
     3481 gacttagttt cttctaaagg ctataatgta aatgaagttg caaacaatgt tatagctaaa
     3541 ttatatgaga tgcaccctgg tatcaatggt tcttatacta caccagaccc aactgaacag
     3601 acagcttatc ttgaaagcat taattccaat gtaaacaaat atgtatctat aataactgta
     3661 gttgcaatgt ttgttggtgg tattggtgta atgaatataa tgtatgtatc tgttatggaa
     3721 agacaaagag aaattggtat aagacgtgct attggagcaa aacctaggtc tatattattt
     3781 caatttttag ttgaagctgt ttttataaca gtatgtggtg gaattttagg aactatagtt
     3841 ggatttgctg ctactaacta tgtttctaaa tatataggtt ttgaagctat acctagctta
     3901 aatagcttat tttatgcaat tgtagctact atattgactg gagttgtatt tggtctgatt
     3961 ccagccttta aagcctctaa actagaccct ataaaggcaa tttataagta gaaattgtat
     4021 aaagattttc gatttttaga ttagtttata taaaacatac ttaaatatat aacgcactat
     4081 tatatatgat agattaggaa cttaatcttt ccaaatataa tagtgcgttc tttcataaaa
     4141 aggattctct tccttactat atctaaatta ccaagatgtt atgttctaat tgttcaaact
     4201 aatacttcca attttgatta atcaatctca ttctcctaat atacaatctt ttactttcaa
     4261 tcattgtaca ctccgcctta aaaaaataat cttaaattat gcgttatata caatctcccc
     4321 attacatatt gtataattaa cctttccata taaagattta cctttaaatg gtgaattatc
     4381 agctttagaa acaaagcttt caactgtcca ttcttcttct ggattgaata taacaatgtc
     4441 tgctacagcc ccttcttcta taaatccaat atttaaattg tacaactttg caggatttat
     4501 agacattttt tctattaact gcatcatact aagatgattt ttatgcacca aatttgttat
     4561 tccaagagct aaagaagttt caagcccaat aattccactt ggagctttta taaactctct
     4621 atctttttct tctttactat gaggtgcatg gtccgttgct attatttcaa tagtaccatc
     4681 atttaatccc tctataatct tatctctgtc ccatttactt cttagaggtg gattcatttt
     4741 tgcatttgtt ccaaactcca atacatcttc ctcagtcaat gtaaagtgat gtggtgaagc
     4801 ttctgcaact acatttgcac ctaagtattt tgcaaatcta accatatcaa cagatacacc
     4861 tgaacttata tgttgtatat caactttagc accagtcttt agtgccaaca tacaatctct
     4921 ggctaccata acatcttctg ctacacttga tgcacctttt aatcctagtt ctgaagctac
     4981 ctttcctgca tttacacctg gatttccaac taaagaaggg tcttcttcat gaaaactaag
     5041 tggtacatcc aactcttttg ccttaatcat tgcctccata attagattgc tatccattaa
     5101 aggtaatcca tcatcagtaa accctggtac accagctttt ttaagagctt ccatatcaac
     5161 taactcttta ccttcaaaac ctttagttat agctgctgct tgtaaaacat ttatacatgc
     5221 attttttgac ttttctttaa cataattaaa agtatcttca ttatctacaa taggattagt
     5281 atttgccata catataacag ttgtaaatcc acctctagca gcagatttag ctccactttc
     5341 tatatcttct ttatatgtaa acccagggtc tctaaagtgt acatgtacat ccactaaacc
     5401 tggagcaact acattaccac tagcatctat tattttttca tattcatcac tttcatcaaa
     5461 cttgcctatt ttataaattt tattttcttt tataattaag tcaatattct catctctttg
     5521 acttttaggg tctattactc taccattttt aattaaaatc atattatttc tcctttatat
     5581 ttttaattta tatagactca tataatatta tacattattt tacatactta aatacaaaat
     5641 tacgtttgac atcaatagcc tttttaggac aaagttcatg gcaacagtaa catcttatac
     5701 atttacttaa gtctataata gcacccttag tccctatctc tatagtggat gcaggacaag
     5761 cttccttgca ttttccacat tttatgcaat cttgaaatct taccacaggc tttggtgtta
     5821 taagctttga aataggttca tttaaaaatt ttggtagctt tacagaaaac aatcttaaat
     5881 ccttacttac atttggaatc ttgtaatttt ttataattaa atcttttata tcttcaccta
     5941 aaatttcaat atctgaaaaa tcttcttgta tatatcctct cttaatagca cctctaattg
     6001 tagcaactct atttggaagt aaacttataa tcttacatgc aacaacatct atagcatatg
     6061 gattttgtga tgctaataaa actcctattt ctcgagtagt accagctgaa ggtccatcac
     6121 cttccatacc tactatacca tccataattg tcaatgttgg ttttatataa tcacaaatat
     6181 ctaaaagtac attttcgcaa aacaactctt ctgtaggaaa tctataatgt atctctgctt
     6241 ttttcaatcc agctatgcat ccatataagt tttttgtacc accagtaaaa gttgccatta
     6301 tatgagtctt taatttacaa agatttatta tataatctga atctactatt ggtttaataa
     6361 tatccatata ttttagaaca tgtgcttttt cactatgtat cttaacatct gaaacatcat
     6421 aatttaactt aatgcccagt tcatttgcta cttcttcaat tccagatgcc ttataaatgc
     6481 tcttaagaga actttgatta tatggaccac cagggctatc tgctattgta atatcacaat
     6541 ttaactctaa aagtttttca catacaactt ttacaaccat aggatgtgta gttgtagctt
     6601 cttcaggctt ttttttcatt aataaatttg gttttataag tactttgctt ccttttttaa
     6661 caaacttatc tataccacca atatcctcta aatttttttc aatagataac tttacttctt
     6721 cataattgta agttttacaa ctcctaacag ataccgactt catattatct ccatccttct
     6781 atcacaacag tttatttgta taatttaaaa aaggagaaac caaagtttct ccttttaatc
     6841 tatgttttaa ttgttttaat tattattttc caaaatgtct gtttaataat cctaagaatg
     6901 ctttaccatg tctagcttcg tctttacaca tttcatgtac tgtatcatgt atagcatcta
     6961 atcctaattc tttagctctc ttagctaatt ttaattttcc atcagttgca ccatactcag
     7021 catcaactct aactcttaag ttttcttttg tatctgcaac tacaacttct ccaagaagtt
     7081 ctgcaaattt agcagcatgt tcagcctctt caaaagctat tctcttatac gcttcagcta
     7141 cttctggata accttctcta tcagcttgtc tactcattgc taaatacatt cctacttctg
     7201 tacactcacc agtaaagtta gctcttaatc cttcgattat ttctgcatct acaccttgag
     7261 ctactcctat tctatgttca tcagcccaaa ccatttcgcc tttcatttct tcaaatttat
     7321 cagctccaac tttacatact ggacattgtg ctggtgcagc atctccttca tgtatatatc
     7381 cacatactgt acaaacaaat tttttcataa ttaatttcct cccaatttta tataattaaa
     7441 tttttatttt taagtttaat aacttgctac aagaatatat ataccccaga ctttatagct
     7501 taaacactaa aaataaaatt tatttcctaa ttaattttat cactttgaaa caccagtatc
     7561 aaccaagttt tgatgtcaaa tttctacata atacataatt tatttgatta attaggaaat
     7621 agttgtcata ttaaatactt tctaatgaag actcctctga atctttaagt ttcacttttt
     7681 tgctctttaa aaaagataat ttagtttctg cagttatgat tgcaacaaat atcaagatac
     7741 taccaataac caattttgct gtaataagtt cctttagaat tattatagaa aatattgtac
     7801 caaatacagc ctctgtggat aatattatag ctgttctagt tccatcaact cttttttgac
     7861 atatagtctg aaataaaaat ccaattgtag tactaaatac agctagatac atagttccca
     7921 tgtaaccaga taattcagct tccatcttta attgacctgc aaatgtttga actacaacag
     7981 acattaaaaa cgctactgta aattgaatag ctgtcaatgc cattgggttg ttatcttttg
     8041 caaactcact tgtaaagaat atatgaaatg caaatccgaa tgaacatata agtgttagaa
     8101 aatctccaaa attaatagag aaatcagctt ctaaagaaag tatcccaatt cctataagag
     8161 ctaccaaact acttattatt cctattttat caagttttct tctataaagt ataaatccta
     8221 tgaatggaac tattacaaca ttagctgctg ttataaacgc atttttagat ggtgtagtgt
     8281 acataagtcc aatagtttgt actgtgaatg ctagaaataa aaatattcct aaaatccctc
     8341 ctgcttttaa taatttttta cccatattgg cacgaatctg tttaaaaaac agtaaattca
     8401 taattatagc tgccaagaaa aatcttaaag ttatgatttg tagaggtgta agcccaccgt
     8461 ctaatgcaaa ttgggttgct acaaagccac ttccccatat aattgctata aatattagtc
     8521 ctatttctcc aaagtatttt ttcaataaag ccccctcctt tttatttttt tgcaaaatta
     8581 taaaccccct aaacagcaag agagtttttc tataagcatt actataaatt aatatagtaa
     8641 attttttcag tattttaaat ctaaatttac atacattttg agctcaaatt tatatccact
     8701 atttaacagt aatatattaa tcattttttg taaacacctt tttaataaat ttttatattt
     8761 tttatatttt tttacctata ctaaattttt ttatagatat ttttaatata tatgatactt
     8821 taatattatg caaataatgt catataagtt acactttagt tatcttagtt acaactttgt
     8881 cggtttactt tttctacttt cttcgatata attataatat atttttgata caattcgaca
     8941 aaagggggaa tgctgtaatt atggtaaaaa aaagaaaaaa gaaaactgtt gcacttacta
     9001 tacttgttgt aatagttatt ggtatttttt ctatggcatg ggcggacatg tctagaaaaa
     9061 aagaaatgaa agaaactcaa gaaagactta aagctgaaag attagaaaaa gaaagaattg
     9121 aaaaagaaaa aaatgaacct attattggtg caaaaaaaga agctgtaaaa gaatatggat
     9181 atgacgcaaa aatagtttgg gacaaactta acaaatacga ctattcaaac aatggagaaa
     9241 agatagtctt tcttacattt gatgatggac catctactac taatactcca caagttctag
     9301 atatcttgaa aagacatggt gttagaggaa ctttctttat taagggtgat tctcttgaaa
     9361 gaaaaggagc taatgaaatt ttaaaaagaa catttgacga aggaaatgca atagcccatc
     9421 actcttatac tcatgactat aaaaaactat atcctaacag aagcctaaat ctcgatacat
     9481 tcgtaaatga acttaacaaa actgatgaag ctatgaaaaa agtcttagga aagaattttt
     9541 cctcaaatgt agttagatgc ccaggcgggt atatgtcatg gaaaaatatg gaacctctag
     9601 gaaactactt aaaagagaaa aacatggcat caattgattg gaacgcttta aatgctgatg
     9661 cagaaggtaa aaagaaaaat gcacaagaat tatttgaaca tgcaaagaaa tcttctgaag
     9721 gcaaagaaat ggttgttctt ttaatgcatg atacttatgg aaaacaagaa acagtaaatg
     9781 cattggacca aattataaca tattttaaag acaatggtta tcaatttaaa attttaatat
     9841 aataaaaaag caagtttatt gaatgtacat tttatactca atagacttgc ttttacttta
     9901 ctctatttta ctagctcgat ttttacaaat tttttctttc ctatttgaac aactaattca
     9961 ctctctttta caatagtgct taaatcctct actttttcac catttacctt tactccacct
    10021 tgagaagcta atcttctaac ttcactctta ctcttaacaa tctcattaga aactagtact
    10081 tcgattaagt caaatccatc ttccttaact tgaattgttt gtatatcttc aggtatttga
    10141 cctttttgga atacagattt aaatctttct tcagcttctt tagcagattc ttctccatgg
    10201 tataatgtta ctatttctct tgctaagtcc atctttatat ctcttggatt tactgaaccc
    10261 tctttcaact gagattctat tttattaact tcatcaggat gaacatctgt cactaaattg
    10321 tagtatttaa ttataagttc atcaggaatt tccatagatt tttggtacat tattcccgct
    10381 tcttcatcta ttccaatata gttacctaaa ctcttactca ttttttcttt accatctaat
    10441 ccttctatta aaggcatcat tattactatt tgagactcca taccaaattc tctttgtaat
    10501 gatcttccca ttagtaaatt gaatctttgg tcagttccac caagttctat atcagcttct
    10561 aatgctatag agtcatatgc ttgcattaat gggtagaaga actcatgtac tgatattggc
    10621 atttgtcctt catatctctt tttaaaatct tctctttcta acattctcgc aacagtaata
    10681 gttgctgcta attttattac atcttcaaaa tttaacttag ctaaccactc gctattaaat
    10741 cttacaatag ttttttcttt atctaaaact ttgaaaattt gttcttcata agtttttgca
    10801 tttgcaagaa cttgctctgt tgttaaagct tttctagctt tagattttcc agttgggtct
    10861 ccaatttttc ctgtaaaatc tccaattatt attactattt gatgacctaa atcttgtaat
    10921 tgtttcatct ttctaagaac tactgtatgc cctaaatgta tatctggagc acttgggtct
    10981 agacccaatt ttactatcat aggttttcct tctttttctg atttaataag cttttccctt
    11041 aactctttct catctattaa atcatctaca cctttcataa ttattcgcat ttgctcgtct
    11101 atcgatttca tttttcattc ctcctaaaat atttatataa tctataattt ttatttttat
    11161 aataaaaaaa gctttcatcc tcttaaaagg acgaaagcat tgcttacgtg ttaccacctt
    11221 agttcatcaa tatctcacga tattaatctc ttagagtaca attacatata ctcgtgtact
    11281 ataacgtgta ctaatccgat atctcttaat ggttgttatc tttcagagat agtgctctga
    11341 gatgtattca aactaaatca atttattcca tttcaccaaa ctggaactct ctgttaaata
    11401 aaatctagcc ctacttcttc tcttcatagc atttatatat tcagattatt tcatcaatta
    11461 caagaaattt ttcttaactt actcataata tattattatt tttatatttt gtcaaccttt
    11521 tttattatta tatacatgtt tttttacatt atactacttt tttatatttt ttaccaatac
    11581 ccaaaattta tttttcaatc aaaagattta tttgtttatt gactatatta ctctgactca
    11641 atcttatatt tattttctta tttaaaaatg cgtttataat tattaaacca ataactgtta
    11701 catcaaatat aaaaaacagc aataaaatta ttatcaaaaa taaaattcta gcatataata
    11761 attttctaaa cttagctatt ataaagtttt gtagtgacat acatgagtct ctaatgataa
    11821 a
//
LOCUS       DAELNY010000116        11727 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.116, whole genome shotgun sequence.
ACCESSION   DAELNY010000116 DAELNY010000000
VERSION     DAELNY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.116"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2013)
                     /locus_tag="KPE55_002572"
     CDS             complement(<1..2013)
                     /locus_tag="KPE55_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG0493626.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMI"
     gene            complement(2036..3292)
                     /locus_tag="KPE55_002573"
     CDS             complement(2036..3292)
                     /locus_tag="KPE55_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0493627.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            complement(3341..5005)
                     /locus_tag="KPE55_002574"
     CDS             complement(3341..5005)
                     /locus_tag="KPE55_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0493628.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            complement(4995..5843)
                     /locus_tag="KPE55_002575"
     CDS             complement(4995..5843)
                     /locus_tag="KPE55_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0493629.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(6073..7125)
                     /locus_tag="KPE55_002576"
     CDS             complement(6073..7125)
                     /locus_tag="KPE55_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0493630.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(7125..7799)
                     /locus_tag="KPE55_002577"
     CDS             complement(7125..7799)
                     /locus_tag="KPE55_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493631.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            complement(7904..8740)
                     /locus_tag="KPE55_002578"
     CDS             complement(7904..8740)
                     /locus_tag="KPE55_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493632.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(8742..9785)
                     /locus_tag="KPE55_002579"
     CDS             complement(8742..9785)
                     /locus_tag="KPE55_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493633.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(9804..10343)
                     /locus_tag="KPE55_002580"
     CDS             complement(9804..10343)
                     /locus_tag="KPE55_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493634.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(10779..11468)
                     /locus_tag="KPE55_002581"
     CDS             complement(10779..11468)
                     /locus_tag="KPE55_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493635.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         3919 a         1989 c         1110 g         4709 t
ORIGIN      
        1 aatcattaaa aatactttag ttgtcttatt tatactcata ttccaacttg aatcttttac
       61 ttctttctta gattttaaat tataagctaa atcttctacc tctccgtatt catcaattat
      121 caatgtatct ccttttaaat cattaacttt acttttattc aacaaataat ccattcctaa
      181 atataagttt ttttcattca ttgaactaat taccatgata tttttttgat tattatatgg
      241 tgattttatt aattgaatag tagaaatatt ttttccataa tcatcaataa agcttatctt
      301 atcatttgat ataaaattgg agtaattttt atcgaacttt atatttaaat tattatttaa
      361 catctttata acagaattat tatgtggcac tccaaaaact acaatattag tatccttata
      421 cttgtcacta aattcttttc ctctaattac atttatattt ccattatgtg aatttatatt
      481 cgcacctaaa gtaacaccta atctaaacat ccaggtcata gcttgtgacc ctgaataatc
      541 tggcattatc acagttaaat cattaaattc atcatctcta acaaatggat acggataatt
      601 ttcaaaagat aaggtttcat tttctttcgt agataacgcc aaataagaat tatttgatac
      661 atatgcccaa ggattattac tctctctagt aacacaattc gaatttttta tactcaaatt
      721 aaatgtcaat ttaacttgat aatagttttt attatctata tctttaggta tttttaattc
      781 taacttatca ttattagaat gtgacctatc caatttttta cttcccacaa taacatcatt
      841 tatagacact gtaactaaag atttttcaaa atctaaattg tcagaatatc ttaaatttaa
      901 gataatcttt gaaccatcat ctaatacctt acccgttggt attttgacat caaacaaagc
      961 ttgttgatta aatgctcctt ccaacaaaaa gtcagaatat cctaaatctt ttaatgttaa
     1021 atgacctaaa tttaattttt gttccctatt aatttttata ttagtctctt tgtttacaag
     1081 tactgatgat gataaaactt ggttagaaag tctattttct attaataaat ttgatgcctt
     1141 tattaaatca tcttcattac taccaattac caccatcatc ttcttattct tattgtatgg
     1201 tgaatctact tgcttgatta tacagtttga agataatagg gtttgttcct ttatacttaa
     1261 aatatccaaa atttcttctg ctgtattttc aggtttacca atatatataa tattatcatc
     1321 tgaccaattt ttcatttctg aatataattt aacatctaac tttaaatcat cattttttgt
     1381 tatttttcca aatgctgatg ccaaattaaa cactgctgta gtctctcctc tagtcatatt
     1441 atctggtact actatagttg tatctaattt atgattattt tcaatttctg catatggata
     1501 cggatattca tttattgagt tttccacctt tttctgctta tatctaattg atatatatga
     1561 ttctttgtga ataaccatcc agttcccagt attggaatca tcttggcaaa ttttatcaga
     1621 tattgttttg tatgttttta tttttatttc attatatcct tgaattatat aatcttttgg
     1681 aactaatact tctaatgtat ttttataatt tgtcttagca tttaatttaa tagattttat
     1741 tggtacatta tttatcaata cagttaaact tgaaacatct ccatttaata tttggctctt
     1801 actaaaatta agatgcaata aaacttcttc aatatcccaa tttttattta cttcaaaaaa
     1861 agtactatta cttcctataa caccatcaat agttatatct ctttcaaact tataattttt
     1921 aactttagtc tcatcagcat ttactttgta tataagtgat atatttgaaa aaaacaatac
     1981 taaagatata attgatataa taaatttttt catgttaatt cctcctgtac atgaatcaaa
     2041 atctttctgt tttataccat ttaacctctc ttttaaaaat tatatctttt agatactcta
     2101 tcattccttt tatagctaca aacagccata gttgagagta tgtaaaatac attattgcca
     2161 ctatgaatat attttcaatt gttgcttcac ctttttctat agtaaggctt atactcactt
     2221 ctataataaa taaaagataa gatagtatcc atattagaaa aaagttaatt ggaatagaaa
     2281 tttctactag tttagatata cttaaaacaa ataaaatatc ggatataatt acagatgtta
     2341 aaaatagaaa atatacagaa aaaaagtaag ctatatcaaa cacaatctta tttcttccct
     2401 gtttaaatat attttttata tatttcatta atacatatat attccctttt gcccatctag
     2461 tacgttgctt tatccacacc ttaacagttt ctggctcttg ttcccatgtt atagattgtg
     2521 gtacaagctt tatcttgtat cccaatttat atatcctaaa acttatttct gtatcttctg
     2581 caattgcttt actatcccat cctccaattt cttctatgat acttctcctc aatataaagt
     2641 tagttcctgg tattgtacat agattaaaaa gttgccatct accagcttgt gacatccatt
     2701 gaaagcttaa tgtttctata tttataaatt tggttaatag gtttttattt ttatttcgag
     2761 tcctaaattt cccaataaca gcacctaatt catcattcat tactatagtt tgtactagat
     2821 atctaagtgc atttttatct ggtgtgttat ctgcatcata aacagctata aaatctcctt
     2881 ttgaaatcgt atatccaata tttaatgcat tagactttcc ttttccacct gttaaactat
     2941 ctgtatttat tatagtgaaa ttataattgt tgtacctatc ctttatattt tctaaaattt
     3001 ctttactatt atcagaagaa ttgtcattta taacaataag ttccatctta ctcttaggat
     3061 aattgagtaa tagcaaagac tccacagttc tacctatcac tttagcttca ttatgagcag
     3121 gaacaagtat tgatatcata ggatattcat caatttcttt tatttcttta tcaaagtttt
     3181 taaaataaaa tatatatcca cccatagcta aaataatatt tataagcaat aaagaccata
     3241 tactaaacaa actaaatacg aacaaatact ctgatacatt taaatctctc ataaatttct
     3301 cctaaaatat tttaaaatat tgattctaat ataaaatcct ttattttaaa aatttctttt
     3361 tatctattct cttaaaatat ataaatataa tcacaaatat taaaacactt atagatacta
     3421 tgaatattaa aactttacta atattggata tggatttaaa tctagtatca cttttagtta
     3481 gttcatctaa gtccttatca tagttgacta taatttcatc tccattattt cttatttgta
     3541 tttcatcaac ttttacccaa ttattaaaat ctcttaaatt cataaattct atattctgtt
     3601 tttctaaata ttctattgtg ttttttagat actctatgtt taaatatgaa tgaaagaaga
     3661 accctccact aaaacctctt acaatggata atttatctaa attttctttt atattttgaa
     3721 aagtaaactt atcttctggg tctatatatc ctaaattttc gggtataaat atattaaact
     3781 cctctgtatc cctaataata taaggatatg tatttgtgct aaatttttta tcattatttt
     3841 gatgttgccc tatataagta gaaaaatatt tttttaattc tttatatccc tctgattcca
     3901 ttgcataatg tggcgcttca aatgcaagag gataaattcc attctctata catactctaa
     3961 gaccattcaa aactctatct ttgacaaata tcttcatatt ttcttttaag ggttcatctt
     4021 ttttaccatc ccaaaattca tacccttctc cagaaacttc ttctttcttg tattgatgag
     4081 tatatccatg caatatcaca gttccacctt tatcttgcat atattttatt gctttgacaa
     4141 tttcagggct ttctgataaa gttattacct tatgattttt aggatttaca tatgctggta
     4201 ttaatgcaat cgtaaatggt atattcttac tacttaaata atccgctatc tctacaagat
     4261 tttttggttc tctaaaagca tgtacatctt ctattcttac aaatatacgt cctttatcaa
     4321 acgtcttaat attaaaaata tcattaagtg aatcgcaaaa tatataaaat aaaactccat
     4381 ctaaatctaa ttttgataca taaaaaagat ttttatcatt tattatatat ggatacttat
     4441 ttaaggtatc attaatactt cctattactt tattacttgt atcttttgat tctacaatgt
     4501 taaaaacata atgttcatcc aagttatatg atttacctct ataatttaca ctaactatat
     4561 tgtttgtttt tcctacatag tctaaattgt attttttatg ttccaaaaga ttttctatac
     4621 catatcctaa ccaacatata gttcctttat atgaatttaa ggaactaatc aagtttttag
     4681 tgtttttatc attattatat gagttttcat tgattcctaa aataaatata tgactgtaat
     4741 aatttttatt tatctcacca tcatagttac tcaattttaa tagctctata tctgaactaa
     4801 atcttcccaa tagagtcctc ataatattta aaatatctct attataagct gtttctttct
     4861 tagaatcata tatgattaat accttatctg tgctggaagt aatttctgat gcatatgaag
     4921 ataagctaaa atttgatatt gtcagtacta ttgataatac aactactaat tttaatagac
     4981 ttttattaaa tattttaaac atcatattga agttcctctt caactatatt cttaaaattt
     5041 atactatctc caaaactact cttatattgt aaaaatgcaa ttttgacatc tatatttaca
     5101 tattttcccc tattgtttaa atctaaattt aattctttta tccttttctt tattctgtct
     5161 ttaacaactt ttgaaccttc caaagatgta tttggcatta gtatccctat catatctttt
     5221 tgtaaactat aaattatatc ttcatttctt gtacattcta taatatttga accaatatat
     5281 tttactattt tattagtttt gttttcacca aatatagtct gtaggtttcc gtagtatggt
     5341 aattttacta tcataagaga aaaatcagta ttatgtctaa tagatttact tatctccata
     5401 tttacatcat tataaaaaat ctttaaattt ctaagtccag tttcactatc aatagtcact
     5461 aattccttat actgttcaga tagtttttta tttgtatttt gcaattcatt tatgcttttg
     5521 ttcagattac ccattataat tgaagatact ggtgttgcta ttatccacat atatgatata
     5581 aattcaacat ccatattgtg agtaatattg ttatataaaa tataggatgt atacataaat
     5641 ataattattg aacttgttat caaccccact actgaatttg ataaataagt taacattatt
     5701 ataaaaaaaa ttattgctaa cattatgaac acattcaatt tattttctgt cctaatcgat
     5761 agtattacta ttccaacaat taaaaatata aaaacgagca aagccaatat taataaatct
     5821 atttttttat ttatcctatt cattttttat aaatttacac ctccaattat tctattaagt
     5881 tgcccatttt ttctaattat tattaaagtc attatacaaa taagttttca tttattaata
     5941 aactatttaa attgcatatg aatataactt aaacaaaaaa taaaactgtt caataattag
     6001 aaaataaatt ttctaaaaaa gaacagtttt attaagtatt tattaagtaa atttacttaa
     6061 aacaacaaga ccctattttg attttaactc tatccaagcc ttgtcatata attttatatt
     6121 ttcaccaata tcttcaaaag tttcacattt atcaagtact tctttagatg gatatgcaac
     6181 aggattctga gttatttctg ggtctaataa ttttagtgct cctgtattag gtgttgaata
     6241 cccaatgtac tcagcattta cttttgcatt ctctgggtct aataaaaagt ttataaacag
     6301 ctcagcttct ttcttatttt gtgctgttgt aggtacacac atagcatcta cccatttatt
     6361 agttccctct ttaggtatag cataatcaag gtcaggattt tgctccatta gtgtaactgc
     6421 atctccagaa tacagcatac ccattgccac ttctccacca agcaatctat ccttaccttc
     6481 atcatttaca taagccaata ctaaatcctt ttgtttcatt aaaagatttt tagcttcatt
     6541 tatttctttt ggatttacac tattcatgct atatcctaat tttttaagag ttatccctat
     6601 agtatctcta acagagtcaa acatcattat atttccctta tactttggat tccaaagtat
     6661 attccagcta tccatagggt ctttaacttc tttcttattg tacaatattc caagagttcc
     6721 ccacatatat gcaacactat attcattagt tttgtcgtat acaggattta aaaaactctt
     6781 atctaaatat ttcatattag gtatattctt aaaatctaat ttttccaaaa gaccttcatt
     6841 tttcattttc tctaccatat agtctgaagg aaatactaaa tcatagtgtg tactaccact
     6901 ctttactttt tgatacatca tttcattagt atcataagtt gaatattgaa catctattcc
     6961 tgtttctttt tcaaacttat ctataagaga ctcatctata taatctccaa cattatatac
     7021 atttaaaact tttgtagaat cagttgcttt attacatcct gtaaataaag taactgtcat
     7081 catacctaca gctataaggg atattgcttt tttaaactta atcattattt aatccccctt
     7141 actatagatt cttttttatt agctagtaaa agtaaaccta gaaccactgc aaacattatt
     7201 gttgataatg catttatttc aggttttata cctcttctag ccattccata gatttctata
     7261 gaaagattgc ttacaccatt tccagcatta aagaaactta ttataaagtc atctattgac
     7321 atagtaaatg caattaaaaa tccagaaact atacctggtt ttatttgtgg taaaattact
     7381 tttctaagag cgtatatagg tgttgctccc aagtccatag ctgcatcttc tatattatca
     7441 ggtaattgct tcatttttgg caaaactgaa agtataacgt atggcaaaca aaacattata
     7501 tgtgccaata gcattgttgt aaatccaaat tccattttta caaatacaaa cagactcata
     7561 agagctactg ctgtaactat ctctgtattt aaaataggaa gatagtttac atttagtatt
     7621 aatttcttac tcttacctct catcttatgt attcctatag cacttatagt tcctactatt
     7681 gtagatatta cagacgctaa tatagctatt acaatagtat aatataaagc actcattata
     7741 ctctcatttt gaagtaattg accataccac ttccatgtaa atccattcca tcttgccatt
     7801 gattttgaat cattgaaaga aaatagtacc aaagcaaata tgggtgcata caaaaataag
     7861 aatactaatg ctaaatatat attagaagtt attttcttta attttaaaat agtaacccac
     7921 cgccttcttt gtcagattca tctccaaatt ttgacattat agacatagat attaatatta
     7981 ctatcatcat aaatatcgat atagctgaac caaagttcca atctccaaca actgtaaact
     8041 gttgttcaat caaatctcca accagcatta ttttacctcc acctaacagt cttgatattg
     8101 caaaagtagt gactgctggc ataaatacca tagttattcc agacattaca cctggcaaac
     8161 tcaaaggaaa tataattttt ctaaaaactg tcatattatt tgcacccaag tcatgagcag
     8221 cgtttatcaa atcattatcc atctttgata gtgctgtata tattggaaga accataaatg
     8281 gtaaaaagtt atacaccata cctaaaagta tagcaaaatt agtatacaaa attgctaatg
     8341 gttgtattcc aaaccaacct aaaaatgtat ttaaaagtcc attttttcct aatatagcaa
     8401 cccatgcata tgttcttaaa agaaagttca tccacatagg aagaataaac aataatatta
     8461 atgaacctct tctacttaca cgagcctttg aaattatata tgccactgga taaccaacta
     8521 taaggcatat taatgtagct cctcctgcta aaagaataga acgcccaaaa actttcatat
     8581 atatagggtc aattacttct ttataattct caagtgtaaa ggcataacct ccacctgatt
     8641 cttttgtaaa actaaaaaat actactaata ttaaaggaat tattacaaat atcgcactcc
     8701 aaactacata tggatatgct aaaaaagatt ttcttttcat cttagttatt ctcttttctc
     8761 attatatgga tatcttctgg atatatatcc atgcctagtt cagtcccaac ctcagcattt
     8821 ttagtattat gaagtatcca caatcttcca ttttcattca attctatctc gtaatgcact
     8881 cctttaaaca ctgtagatgt aactttcccc tttaacattc cttcttctgg ctttaccatt
     8941 tttatatctt ctggtcttat tactacttct atattttcgt ttttttcgaa tcctttatca
     9001 acacactcaa aatctctatt acagaaatta actttataat cttctatcat aattccatca
     9061 aatatgttac tttcacctat aaacttagct acaaatgaat ttttaggttc attgtatata
     9121 tcttgtggtg ttcccatttg ttgaattttg cccttataca ttacaacaat agtatctgac
     9181 atagtaagag cctcttcttg gtcatgagtt acaaaaacaa atgttattcc taattttttt
     9241 tggattctct ttaattctat ttgcatctct tgtcttaact ttaaatcaag tgctccaagt
     9301 ggttcatcta gtaataatac ctttggttca ttgactagag ctcttgctat tgcaattctt
     9361 tgttgttgtc ctccactcaa tgattcaact tgtcgtcttt caaaaccttc tagtgcaaca
     9421 agtttcaaaa cctcttttac tttagcatct attacatctt ttggagtctt tttaattttt
     9481 aatccaaaag ctacattttc ataaacatcc atgtgtggaa ataaagcata tttttgaaat
     9541 actgtgttta cttgtctttc atatggtggc aaattattta tctccttacc ttcaaataaa
     9601 actttaccat catcagcata ctcaaatcct gctattattt ttaatgttgt agtttttcca
     9661 catccacttg gtccaagcaa tgttagaaac tcatttttct ttatatctaa attgaaatta
     9721 tctaaaacag taagttcttc ataatgttta gatatatccc ttaattctat tatattttct
     9781 accaattatt aaacacctct attctaaaat gatggtggcg tacttaccca aatcactttt
     9841 gctgtagttt tgccctcgtt tgatatatag tgatttgctt tcggtctaaa gtaaaaactc
     9901 tctccttttt ttactttatt tttcttttcg cctatatgga catatattga acctgctaat
     9961 acatatccaa attcttcacc ttcatgtggc ttttcttctt tatactgtcc tcctggatac
    10021 aatgttatta ttattggctc catttcattt ttttgtgaat ttggaattaa ccactttaat
    10081 ttatatttta aatcttcatc ttcagtttca aacatatctt cttttgtaaa actgattctt
    10141 tcatcatcta tttcattaaa aaattccctt aaatttgtac ctagtatttc aagtatatct
    10201 ataagggtag ctatggatgg tgatgttaag ttattttcta attgagaaat aaatcccttt
    10261 gacaattcac atctattagc taactcttcc tgggtcagtt gtttttctgt tcttaatctt
    10321 tttatctttt cacctatatc catgttgcac ctcctagttt ctgttttatt ttactaaaca
    10381 ttttgtttac taaacattga gtttaaaatt tctaaacaac ataggtaatt atatataaaa
    10441 tttggatata atacaagagt ttttttgaca tttttttatt tttttattta gaataaatac
    10501 aagcatttta aaggctttgg aaaatacaca gtttaaaggt gtttatatta atatctataa
    10561 agtttagtat ctctaattat aaattaattc attcattaaa tagagaataa gacttctagt
    10621 actaaaaaaa tattgaacaa ataaaaaatt actagaaatc tatattttat agtgttctag
    10681 caatcctcat attttaatat tcttttaata ttcaatttat ctatctaaac aaaatgttaa
    10741 tttaaaatac ttaacatttc gttgaattta ttcttaaatt acttaactat attttgaaca
    10801 gcagcttttt tctcaccact catatacact ttgataaatt ttatatcatc tactataaat
    10861 ctatgtttaa tcttactaca atctaattta aataagccaa ataccatttg tgtgaaatac
    10921 cccaatccaa atgcacttat tatagttcct ataccaactt ttccacctaa taaaaatcct
    10981 actattaatg ctccaacttc tatagtacct cttattaatt taagtgattt tcctgttttc
    11041 ttttgaattg caaccatcaa accatctcta ggtccacttc ctaaaccact tcccatataa
    11101 aataccattc caacagacaa aagaaccaat cctaatatca acataattat tcctacaaat
    11161 aaattatcag cctctggaat taaattacta tataaaagta aatccataaa aaatccaatt
    11221 agtagcatgt taaaaacagt tccccatcct acattttctc caagaacgac atcagcaata
    11281 actactatag agcctacaat tatagaagca gttcctatag ttattcctat atgatttgaa
    11341 agtccttgat gaaatacatc ccagggagat aatccaaggt tacaatttat cgtcaatact
    11401 ataccaactg aacatataaa taaaccgact actagcttta aaaatttcca taatactttt
    11461 tgcaacaaat tctacccctc ctaaacataa tttaactact cactctaaat atcaaaagcc
    11521 agcagttttc ttacaaaaat cttattttat taattttata atatacttct atgatacatt
    11581 attttatgct aaataactac cattttatta caacttagtt acaatttttg tcattatttt
    11641 ttggtgaagt aatttataca tttatccaac caatttctat ccttcaaatg ttttcaattt
    11701 catttatatt atttataaat gaaaaaa
//
LOCUS       DAELNY010000117        11621 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.117, whole genome shotgun sequence.
ACCESSION   DAELNY010000117 DAELNY010000000
VERSION     DAELNY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.117"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..540)
                     /locus_tag="KPE55_002582"
     CDS             complement(<1..540)
                     /locus_tag="KPE55_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0493636.1"
                     /translation="MFKKIVAMASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNV"
     gene            926..1660
                     /locus_tag="KPE55_002583"
     CDS             926..1660
                     /locus_tag="KPE55_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0493637.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFAFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1803..2294)
                     /locus_tag="KPE55_002584"
     CDS             complement(1803..2294)
                     /locus_tag="KPE55_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0493638.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            2643..3026
                     /locus_tag="KPE55_002585"
     CDS             2643..3026
                     /locus_tag="KPE55_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493639.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            3532..3915
                     /locus_tag="KPE55_002586"
     CDS             3532..3915
                     /locus_tag="KPE55_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0493640.1"
                     /translation="MNLTKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3917..4267
                     /locus_tag="KPE55_002587"
     CDS             3917..4267
                     /locus_tag="KPE55_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493641.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(4368..4826)
                     /locus_tag="KPE55_002588"
     CDS             complement(4368..4826)
                     /locus_tag="KPE55_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493642.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4871..5659)
                     /locus_tag="KPE55_002589"
     CDS             complement(4871..5659)
                     /locus_tag="KPE55_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0493643.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(5798..6235)
                     /locus_tag="KPE55_002590"
     CDS             complement(5798..6235)
                     /locus_tag="KPE55_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG0493644.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            7057..7566
                     /locus_tag="KPE55_002591"
     CDS             7057..7566
                     /locus_tag="KPE55_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493645.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIGFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFVTRKKI"
     gene            7971..8507
                     /locus_tag="KPE55_002592"
     CDS             7971..8507
                     /locus_tag="KPE55_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0493646.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(8941..9741)
                     /locus_tag="KPE55_002593"
     CDS             complement(8941..9741)
                     /locus_tag="KPE55_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493647.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(9936..11534)
                     /locus_tag="KPE55_002594"
     CDS             complement(9936..11534)
                     /locus_tag="KPE55_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0493648.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         4195 a         1659 c         1521 g         4246 t
ORIGIN      
        1 aacattgcct gcttttagat tttctccaat agtaagacct ttttctttta gaacatattc
       61 aaaagtcatt agtggaactc caccttttct accacctaat atttcggttc cttttaaatc
      121 attgtaagag aaattaggca tttttttcct agcaactaga aaactaccat ctctttttgt
      181 caattgagca aagttcactg cataatcgtt tttgttttga ttatatacat atatagatgc
      241 ttcaggaccc attaaaccta cgtttgcttc acctgaaatc aaagctgcca tagttttatc
      301 tgcaccttgg gtatttataa ggtcaatctt tataccttca tcttcaaaga atcctttcgt
      361 aatagcagca tactgtggag cataaaaaac agagtgagta acttctgcta tagttacttt
      421 atccagattt ttagacgaag aagattcatc ttttttgtca ttagagttaa aatcacatcc
      481 agttagacat attgtactta caatgataga gcttgccatt gcgactattt ttttaaacat
      541 aataaatacc tcccataaga catacgaata gtatatagta ttaaaaattt attgtagttg
      601 ttacaaaaat gtatctatgc ttataatatt tctacaaaaa cttaataata attttcagtt
      661 ttatgtttag tttttttagt aatgtgtaat attggataaa ttatattgat tatttataaa
      721 ctaagtaata aaattagaat aatatcactt ttttgtctat tatatataag gaaaaatttt
      781 ttaataatat aaatcatatt catttatatt attaaaaaat ttatttatag aaacttttat
      841 tattttatat aaaagtgata aaaacttata ttttgtgttt agtagagtgt tgctaagtga
      901 atatatttaa aatggaggaa actttatgaa acaaaataaa tatgatgatg atagattttt
      961 taataagtac agtaaaatgg aacgttctac aaatggatta gcaggtgctg gagaatggca
     1021 tgttttaaaa aaaatgttac ctgatttcaa aggaaaaaga gttttagatt taggatgtgg
     1081 atttggatgg cattgtcaat atgcagttga gaatggagca atttctgctg ttggagttga
     1141 tatttcagaa aaaatgttaa aagaagctag aaacaaaacg caacttgata atataaatta
     1201 catatgtatg cctattgaag atattaattt tccaaaagat agttttgatg tagttattag
     1261 ttccttggca tttcattata ttcaatcatt tgaagatata tgtaagaata taagtaattg
     1321 tctttcaaat aaaggtaatt ttgcattttc tgttgaacat cctatattta ctgcatatgg
     1381 aagtcaagag tggtactgtg ataaagatgg caataatcta cactggccag tagatagata
     1441 ttttgaagaa ggagtacgta ggtcaaattt tctaggagaa gaggttataa aatatcataa
     1501 aacattaaca acttatttaa atacgttaat gaaaacagga tttgaaatat tagaaattat
     1561 tgaacctcaa ccagaagaaa aactgttaga tacaattcct gatatgaaag atgagctacg
     1621 tagacctatg atgttacttg tatctgcaaa aaaaagataa gtaaatataa ataatcatta
     1681 ttgtagaaaa aaagttgtag aaaataatta cactaatgga gatgattata ccagtggtat
     1741 taattacacc agtagtaagg aggcaaaagc attcgttgct tttgccattt tttaagttgc
     1801 aattatactt ctacgtagta tatttcgttt ccattttctt ctataagctt tttcatatct
     1861 tcaaatttta aaaatacagt agcagtatta tcatttggat gtatacctat tttatccatt
     1921 tttaataaat ccttatcaaa tacaactttt acaacagcct ctttatcatt taatatacca
     1981 aaaggagtca cagctccttt agtaagtttc aagtattttt gaagtctttc ttctgatgca
     2041 aaactaagag gtttactatc aatttgttga cgaatatctt ttaaatctgc ttttttgtct
     2101 ttacctaata caactaaaaa atgttctttt cctttagcat cacgaagaaa taggtttttt
     2161 acaacaaatt ccatatgtaa tgtttttaat tcttccattt cttcaattgt ataaactggt
     2221 ggatgattta cagcctcata aggtatgctt aactcatcta attttttata tacttcctgt
     2281 tgttgattgt tcatgatttc ctcccattag aaatttattt tatatataaa taatattatc
     2341 acagaaaact ttaaaagaaa acgttttata taatatagac aatagattat atttttataa
     2401 agaagcaaga atactcaata aaacttaaag aaattaaaca atttctaaaa ataccttgaa
     2461 aatagaacct tatttgccat ttataaatta tataaaaagg gattatataa agtcatatat
     2521 gaaaaaacta tattatacta tcttttcttt ttggaataaa ctatacttta aatatagata
     2581 tttaatttaa ataaatttta cagtattaat agtaaattat cttagaaagg aatgtgcttt
     2641 atatgtctac agacaaagtt gttggaatca tagatgaaga aacggcagaa cttgcaggaa
     2701 ttgaatatac tggtaaaatt tatgcttctt ctggtgttat aaaacatata aagaaaaaac
     2761 atagatgtca actgagtaag gatattttta atgatataat agatactata aaaatggtat
     2821 taaagtcacc tgaatatata ggaagtcatc ctaaaaaacc tggaaaaagt gtggagttta
     2881 taaaaaaaat tgatgattat attttagtag ctacagaatt agatacaaaa aaaggatatt
     2941 tatatgtatc ttcaatgtat gctataaaag aagcaaaatt agaaagtaga gtaaacagtg
     3001 gaagagtaaa acaatatgat ggttaacata atattattaa ttttccctaa aatattgaaa
     3061 aatattatta tataagttat aatattaata tacacgtaga tatttattag ttagatttga
     3121 ggttggaaaa ggcacccaat acgctactat agagtagtta atagagatgc aggatacgcc
     3181 gccctgccaa atctaattaa attaaaaaga gaggattaat tcctctctta ttgttttttt
     3241 gagatactaa tttaatctat atagaaagat tgaaggtcat ttatactaaa agttttcagt
     3301 tatatacatt aaactaatta aataagatat ttataatcta tatgtgatac atattatata
     3361 ttaataaaag aaaaatatat agaaaatcct tataaaaatt taaattgtaa tgaataggaa
     3421 gtaattaaaa agaattttta ttgattttta aaaatttagt tagaaactat atcattatgg
     3481 taaaaaatgt attataatgt ttagaaatat tataataagg agcgattaaa catgaatcta
     3541 acgaagaaaa tagaagataa aagaaaagct aaacagagag ctgaaaaagt taagaaagct
     3601 aaaattgcta cagctggtgt ggtattagga gctgttactg gtgcagtgag tggagtactt
     3661 ttggctccta aatctggaaa agaaacaaga gaggatataa aagatgcttc acaacaaatt
     3721 gctgaaaaga taaatatgaa aactgtagat gtaaaaggca aagtatctga gaagctagag
     3781 gataaaaaag gaaactttat agaatcaaag aaaaaaataa aaaaatatat agataacaaa
     3841 aagagtgttg aaaattctag tgaggaagat aaaataatag atatagttga accagttagt
     3901 gttactgagg aataatatgg acaatacaat aacgattaac ataagtataa tatatgtatt
     3961 attagctata cttgcttgta ttgctttagt ttatttaatt attgtacttg ttaaactaca
     4021 taagatttta aataatgttg gaactatgtt aaatgaaaat actcaaaata taaatacaac
     4081 tttaaataat cttcctaatg ttgtagaaac ttttggagaa gtaggagaaa atatgaaaga
     4141 tgtaacagaa gtagttactg aagttacagc agattttctt gttactaaag acagtgtcaa
     4201 atcaaatatg gatgttatag ttgatatatt gactataatt aaaaacattt ttctagataa
     4261 aaaataataa gttaatataa aataagccta taagatataa tttaaacgac cttaaaatag
     4321 catttataag gtcgtttttt attagcttga tttgacacta tgtgttttta ataattggta
     4381 atattcctct ttatcatttg aacatatata tctatgcttg ttgtttcaat tacatccata
     4441 agaacatctt tacattcaag ctcaaaatct tccattcctt ctaaatattc atcatcttca
     4501 tcaaaaaaga tataagaatt atatagccat tcttcaaatt tttcttcaag tatatcccaa
     4561 tacgaactcc aaaataatcc acaacctgat atataagatg ctctactttg atttatataa
     4621 acctcattta gaagcttttg taatctactg ctataaagaa aatctgctct tccactgctt
     4681 gatattaatt catttaaatc ttctattgac attaattcaa ctttaccact tttatcataa
     4741 ggagtataat tttcatcaac ccatttatgt ggtttgtttt ctctgcatac tgttgctatc
     4801 aactctacaa cttttgttct tatcattttt ttatcctaac ttatttattt tttataaaat
     4861 aatcatgatt ttataattta tctatttctt gtaatgctaa tttaacagtt tcatatctat
     4921 cagcaccatt tattactaca tcacctttta caagtttatt agcttctcca cctacaacat
     4981 aaaccttttt acctaaacct tgcttataac tagacgcctc aattattaaa gaatctttca
     5041 attgccaatg taaaatctga gcagccactt tatcagcatc acctgtataa acgattacat
     5101 tttcatacac tttattttcc cctccccctt gagaatttga tatagatttg tttagtatac
     5161 cttctgctat caatttagca actttatctt tattattaac ataataatct gtatctgctt
     5221 tactatctac aaaacatact tctattaata ttgctggtgc aatagtatga ttaagccaat
     5281 aaagacctct aacatcagac tttgcacccc tatctttgaa tactgtagct aacctagtat
     5341 tcactctttc tgcataggtt ttgccattat tagtcttata tattgtttct gtccctactg
     5401 gagttgaagt tgtagcattt gaattaaaat gaatctgaac agccaaatca gtttcctgtt
     5461 tatttgctat agcacattgg tctgctaaat gattacttga ttcatcaact cttcctgtat
     5521 aaacagtagc tccacctatt ttaagccatt taacaattaa gtcattcagt attcttgttt
     5581 ctttgctttc gtttatatac ccaacagctc ctgtaccttt tccaattaaa gtgtgccctg
     5641 gtattattac tactttcaaa ctaacatctc ctttacaatt aaatttaact tctatttaat
     5701 atatgcataa cctagaattt tgtttctttt tatatcttat acttttatta taacctattt
     5761 tgtaaaaata tagaatatta agtaaataat gttaaaatta taattttgta tataagacat
     5821 tacttgattg caataatttt atagcatatt catctattct atatccagtt ctataatata
     5881 tatgtttaat tccagcttga attatagcct tagtacaatt taagcaagga aagtgagtta
     5941 cataaataga gcaattttta actgaaatac cttcttttgc acaataataa agggcatttt
     6001 gttcagcgtg aatggttcta atacaatgtc catctctcat ttcatgacca acttcactac
     6061 aatgcttatc tccagaaata ctaccattat atccagtaga cactattctg ttttctgaat
     6121 tgacaattat agctcctaca taagctctat cacaagttcc tctttcagct actgtttcgc
     6181 acagtctcat aaaatattct tgccatgagc atctattttc aagtttttta ttcaaattat
     6241 atacctccaa aacttatatc taatatataa gatttatacc ttaaaaataa tattaaaaac
     6301 aaatttatac gataaataaa atttattaat actgttttta gtctaaatta tttcatttta
     6361 aattaaagtt acaaggaatc taaaattata tatatctttc ttcgtaatat taatgttaca
     6421 atataaaaaa ctgtctcaat aaaatagaga cagttttaca gtcaaagttt ataatacaag
     6481 ttcatatttt tctttaaatt cttttacagc ttcgggccat gccttttgtt ctgtaatacc
     6541 tttttcttta gctattttaa ttgcaatctc tcttatcatt gcagcgcctt ctaatattcc
     6601 catttttaat ctcccttcaa atatcttttt gtatagaata taaatagtgc taagatttat
     6661 tacatgctgc atatacaata tctatacaac acataacttg taaattcctt ttattttcat
     6721 acgtctttaa ccgacatata attatattca ataagtttta aaattatgaa ctcaattata
     6781 attattttta tttttttaga atctaaacac taaaaattgt aggtaaattt gtcttatgcc
     6841 tttataaata tttctacagt tttaattact ggacatatct atttttatta taaaaagcgc
     6901 ttaaaaatcg agaatatccg cataaaaact gtatataagc aaaacggtta aaggaaaaag
     6961 aagaaattta cttgaatgtt aaaatgcgtt gcttgaagca atgggcagtt ttgcctataa
     7021 tatcggtaac aactgaaaga aaggtggtta gccctaatgt taagcgaaaa cattaaaaca
     7081 atcaggaagt caaaaggact ttcacaacaa gaacttgcta taaaactgaa tgtagtgcga
     7141 caaacaatat ctaaatggga acaaggactg tcagttcccg attctgatat gttgatttcc
     7201 atatcagagg tgcttgaaac gcccgtaagc actttacttg gagaaactat tgttgagtcg
     7261 gaagttgata gcttaaaagt gatttctgaa aaattagaga ttataaactt gcaacttgca
     7321 caaagaaaaa acatgagaag aaaaatgttt cattggttat ttattttatt atgtgcagtc
     7381 atattaatag gttttgtggt cttaggggga ctaaatagtc cttacttgga ttggaattat
     7441 accgaccctg aaaccgctgt ttttggagta gcttttcatg cttttgagtg gctatttgtc
     7501 agattagtac cgattgtcct tataggagca attattggaa tctttgtgac acgtaagaaa
     7561 atatgaagta actctgcttt ttataataca gcaagcgttc cttaaaagcg tcattctaca
     7621 tggcaatcga ggacgactat gtaaggaaga acctttttga tttccaacta agcgaagtta
     7681 ttgaggacga cagtaagacc aaaaccgcat taagtaaagt acaggaagca agactgctgg
     7741 acttatactc tcatgcgtca gatgcaggaa atgcgaggtc tggtagcatg atttacaacg
     7801 atttgtacaa cgactgtgag tagaaatata tggagataag tattatctac gaaatcagaa
     7861 aagaaatgac ttgataattt actcatgtta taattattga tgtgaaatta ataaatcgaa
     7921 attccttttt tggatttaac aatactgagt aaggaaaaag tgaatgactt ttgcaaggag
     7981 gtgttaatat caggcaagta aagtcttttg tttttccagt tatatcctta atattcttag
     8041 accaaattag caaagttctt ataggattat tcttaatgga ctttgaaatt gatataattg
     8101 ggaaattttt aagattcaat cctgttcaaa atacaaatct ctcttatggg ggaaacttta
     8161 ttggtattct atctaattta tgggtattgg tattgtttaa cattttagtt atattagtta
     8221 ttatatctgg atatgctttt tataaatcaa aaaatgaaca aacaagctat tcagtaaaag
     8281 taattatgtc ttgtggactt gctggtacaa tatgtagctt gatagataaa ttattttggg
     8341 gaggaagttt agattttttg cagataccaa gcttttttat tttcgactta aaagactgct
     8401 accttactgt tgcagaaata atatttgttg tcataggaat tttgcataat agagaaatat
     8461 caatgaaaga atacatatat ttttgctatc gtcagtttaa aaaataaatt tagaagataa
     8521 atttaagttt caaggtaaac ttatttaatc acagcaaata gttggcttac ttaagctttt
     8581 gggctaaact gtgagaacta attaagtctc tgctatctaa caaacagagc acaaaaataa
     8641 aaattaaata ttaaatataa agcacagtta attttgatta aaaagttgac tgtcttttct
     8701 tttgtagaaa tttaagcata ggtaataaag aaatgactgc atttaagcat tatgtatata
     8761 attggaggca ataaaaagcc agcaaagccc ataaaataga gctgtgctag caacatgatg
     8821 agttataaga agataatcaa aaacttacgc tttattttaa tttaaaaaaa tgattactgt
     8881 aaagaaatca ttttttatta taatcactga tacatcaaat attctaaata aaatttttat
     8941 ttatctggaa ttgtatattt tttctataat attaactaca gcgtacattg cccaagcaca
     9001 gatagccaat acaaatactc ccatcattac taaatctaat ttaaatactt gagaaccata
     9061 tactatcaag tagcctattc catatcttga aactaaaaat tctccaacta ttacacctac
     9121 ccatgccata ccaatattaa ttttagttaa atttattaaa tttcctgtgt ttgcaggtaa
     9181 tatgagttta aataatattt gagatttagt agctccaaag ctttttaaca tcttaatttt
     9241 ttcttcatct atattcataa aataattata agctgaaagt atagtaacaa caacagaaat
     9301 cgttacagct gttacgacga ttccttctat tccagcacct acccaaacta taattatagg
     9361 agctaaagca gtcttaggaa gagcatttaa aactaccaaa aatgggtcta atatttttga
     9421 taatttttct gaccaccaaa gagcaattgc taccaaaata cctaatacag tacctataac
     9481 aagaccaaga acagtttcat atacagatat tccaatatgt ttaaataatt gaccactaaa
     9541 tgcatattga ataaataaat tgtaaatatc acttggtttg ctaaatagaa atgtatttat
     9601 aacatttaag tcagctaaaa gttcccagat tacaataaaa ccaattaaaa ttaatagctg
     9661 gtagaataat acatttcttt tttctctttt tacaccttta acatagttta tatatccttc
     9721 agatctttta ttaagcctca tgtgtatcta gctccttcca taatatgtcg aaatactttt
     9781 gaaaatcagg aactttacgt gttttgagag gcgtttttgt tccatccatg ttaagttcaa
     9841 tatcgtaaat attttttatg gtagctggtc ttttagatag tattgcaacc ttatctgata
     9901 tgcttattgc ttctgctata gctaaaaaag tgcaactaat tgcttaaaga aacagaatta
     9961 tccacttcag cacccatata aacaatatta agatttccta aactgctatc ccaaacgata
    10021 ctattaataa tagtagataa taacaatctc ttatcatcta aactggcatt atcaacttca
    10081 tcattaaatc tcttcaaatt actaacaata atatcaatat taatactgtc actactaata
    10141 ttgatgttac tagattccaa aatattaatc ttatccttta aaatactaat ctcattattt
    10201 aggctctcaa tctgggaaac tatataatca gaagcagtac taccagtatt ttttgcaagc
    10261 tctataacta aatttttaat aagtctttcc ttatctaaaa tttgattttt aagtaaatta
    10321 gtctcagatt taaaaatatt aaaaacatta tcagtcttaa tgttattact attgaatgca
    10381 cttataatag actcttcttt acaattctta attttatcta taactaaact ttcaactaat
    10441 ggaccattaa gatttttaca gttgcaacta tcagacccaa gtgttttttt agtaccacaa
    10501 atataataat actttaaatc cttgtcagtt tttttgcctt tataggtaat tctcatatta
    10561 gaaccacatt taccacattt tataagacca gataaaagag cagtttttcc agttgatatc
    10621 cttggagttt tatcacgatt tttatctaat tgattttgta cttcaagcca tatttcaggt
    10681 tctatgactc catggtgttt tgcaattgct gctattgagt tagatgtatt tttagcataa
    10741 gtaaggattc cacattttct atctggagta cctacaacat ccataccact atttttaagg
    10801 taatctaaaa tttcatcatt agcttttaca tatgctggat ttctaagtat tctagataaa
    10861 gaactagggt caaatttacc accacgagga ccccttattc cattattata gacatattta
    10921 tataacttag ataaggacct aagttctaaa tatttattgt atattagctt tacaatctcc
    10981 atttgttcat catttacttg aagcttatac atacttcttt cttttagatt ttcatctaag
    11041 aatgtgattt tttcactgtt gaatccatga ggtgtattgc ctccaagcca tctaccacta
    11101 cgggctaatt catacatatt gtcttttata cgttctgcta ttgtttctct ttctaattga
    11161 gcaaatacac ttgatataaa catcatagct cttcccattg gagttgaagt atcaaattgt
    11221 tctttgatag atataaatga tatgtttagt tcatttaatt tttctattaa agtagagaag
    11281 tcagatacat ttctcgatat cctatctaat ctataacaaa ctaggtaact aaatttctta
    11341 tctttggcat cctgcatcat tttcttaaat tgtggtctat ctatattgct accactaaat
    11401 ccctcatctt catatatcaa aaaatctttt acaccaatag actttgcata atcaatacat
    11461 aattgtattt gattttctat agaatcacct tttccagtaa atcttgattt tcttgaataa
    11521 attgcagctt tcaaaattaa aaccccctat aagttaggat atatatttga aattatttat
    11581 atacaacctt ttaagtaaac ttattagaat gaatttatta a
//
LOCUS       DAELNY010000118        11610 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.118, whole genome shotgun sequence.
ACCESSION   DAELNY010000118 DAELNY010000000
VERSION     DAELNY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.118"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            304..825
                     /locus_tag="KPE55_002595"
     CDS             304..825
                     /locus_tag="KPE55_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0493649.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            1204..2379
                     /locus_tag="KPE55_002596"
     CDS             1204..2379
                     /locus_tag="KPE55_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493650.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2414..3952
                     /locus_tag="KPE55_002597"
     CDS             2414..3952
                     /locus_tag="KPE55_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0493651.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3984..5186
                     /locus_tag="KPE55_002598"
     CDS             3984..5186
                     /locus_tag="KPE55_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0493652.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(5353..5952)
                     /locus_tag="KPE55_002599"
     CDS             complement(5353..5952)
                     /locus_tag="KPE55_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0493653.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            6122..7480
                     /locus_tag="KPE55_002600"
     CDS             6122..7480
                     /locus_tag="KPE55_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493654.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            7473..8105
                     /locus_tag="KPE55_002601"
     CDS             7473..8105
                     /locus_tag="KPE55_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0493655.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            8434..9591
                     /locus_tag="KPE55_002602"
     CDS             8434..9591
                     /locus_tag="KPE55_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493656.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(9738..10373)
                     /gene="lexA"
                     /locus_tag="KPE55_002603"
     CDS             complement(9738..10373)
                     /gene="lexA"
                     /locus_tag="KPE55_002603"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG0493657.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(10477..11604)
                     /locus_tag="KPE55_002604"
     CDS             complement(10477..11604)
                     /locus_tag="KPE55_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0493658.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         4361 a         1425 c         1897 g         3927 t
ORIGIN      
        1 aatactattt tactgataat cttttcctta tcactaatct tacattttaa aatattattt
       61 ttactcgtaa tattatcata agaatttgtt tcttatttaa aactaattag aattataaag
      121 tttattgcca tgttattaat aaaataaatc cagttaaatc gagtgctagt taaagtaata
      181 agaaactttt acaatggttt cctttatcga ttgaaataaa agttaatatg gtataatatc
      241 taaagagaga atcttatcaa ttagaatatg catagaaaca agtggcttaa aaggagacgt
      301 tatatggaag agatagtaaa gaaggaacaa gcaagtatga atgatatttt tttgtatctt
      361 aaagctaaat caaactcatt atataaattt gtaatattat ataatgagtt tatcaataaa
      421 cctaaagatt atggaactgg ggaacttata aacatggttg aagtacatat gctaacagcg
      481 attgaagaaa atccaggaat aagtccaact cagttagcta aaatatggaa cagaactaaa
      541 ggtgcaatat ctcaaactat atctaaattg gagaaaaagg gttatataac taaagaaaag
      601 aaagaaggaa atgcaaaaac tatattacta tatccaacca catcaggagt agagttaagt
      661 aaggcacata aactatatga caccatagat gttacaagaa catttgaata cttaatcaag
      721 gagtgtacat tagaggagat agatagtttt tataaagtaa ttgatttgtt tattaattta
      781 ttagaagaat ctccgcagga aaataatgat tctgaaaaag tgtagctgag attgaagttg
      841 aataaagaga aatagatatt atagaagtag tacttaagta cacattatat aaaaatataa
      901 taggtatagg attaaaaagt ttagtggttt aaagtttaac cactaaactt ttttaatgtc
      961 tataatctac tattatttaa tttactaaaa ataaaaaaac tcattatatt aagataaaaa
     1021 tagaaaaata actagaaaaa tactataata tatcagctta atacagaaaa aagttaaaaa
     1081 atttgagaaa aataaaaatg aaaacgtttt atattgacat agagaaaaag tagtgatacc
     1141 atttaaatat agtttagtag ctaaactata tttaaattat aataactatt ttagggggat
     1201 gttatgaatt taaaacaatt aagtgaaaaa tacgaagaat atgtaattca aatgagaaga
     1261 gaatttcata agtatccaga attatctttt aaagaaataa gaacaaccca aagaataagg
     1321 gaagaattac aaaaaatagg ggtccctttt gtatctattg aaccaaatat cgtaatagct
     1381 actatacaag gcaaaaatga aggtaagaca attgcaatta gaggagatat agatgcgctt
     1441 ccaatgcaag aagattcaga tgttgaatac aaatcagaat atgatggaat aatgcattct
     1501 tgtggacatg atgctcatgg agctatgtta ctaggacttg cacatatgct aaatgatata
     1561 agaaaagatt taaaaggtag agtattatta gtttttcaag cagctgaaga agttggtggt
     1621 ggacacaagg agataataca gtactttaag gaaaatggag gaccagatag attaatagct
     1681 actcatgttt ggtccgatat tgaagctggt aaaatttctg ttgaaccagg acctcgtatg
     1741 gctggtgggt ctggttggag aatagacata acaggtagag gaggtcatgg ttctagacca
     1801 gatcaaagca tagatcctat aaagccagct tgtgatatag ttttaaaagt atcttctata
     1861 ccagttaatc atataagtgt actagaacaa tgtgttgtac atgcatgtgc aatacatgga
     1921 ggaactacaa taggaaatgt tattccaaac aatgcagagg taataggtgg atatagatat
     1981 tttactgaag aatcaggcaa aaaagtgttt gacattataa agcaaatgag taatggtgtt
     2041 ggacaagttt atggagtgga tgtgaaggtt actcatactg gaggcatagg acctgttatc
     2101 aatgatgaag aagcagttaa gatggctaaa gaaatagttt ctaatataga tggattggag
     2161 ttagattctt atgagccaat atgtgcatca gattgttatg gtgaaatatt aaaagagtat
     2221 tctggctttt attgttatct aggggtaggt aacaaagaaa aaggaataat atatgcacaa
     2281 caccatccta agtacaatat agatgaagat acattaaagt taggttgcga atttatggct
     2341 aaatatgcag ttgaattttt aaataaaaat aataactaaa ataaatcgaa ttttataaat
     2401 gggggggatg aaaatgcaag gtgcattaaa ggaaaataag aaaaaaagtg gtggaggatt
     2461 tttaggatgg attgaaaaag ttgggaataa aatgccacat cctctagcat tatttacata
     2521 tattacattg atagtagtag cattatctgc aatagcaagc tttttaggtt tttcagcaac
     2581 aagtcctgct acaggaaagt taataaaagt tatgaactta ataagtgcaa aaggtttagt
     2641 actatttatg caagagtttg ttaagaactt ccaaaacttc cctgtgcttg gagtagtttt
     2701 agttatggca gttgccacag gagtttgtga aaagacagga tttttttcaa ctgccataaa
     2761 aatgagttta tcaaaagtta aggggaatgc ggttgtattt ataatagctt ttataggagt
     2821 atttgctaat caagctggtg atgctgcttt tgtattagta ccaactcttg caggagcaat
     2881 attttatgga ttaaatagaa atccattagc tggtatattc tgtggatttg catcagttgg
     2941 tggtggattt gcaacagctg taattcctgg gggatgggac gttacgctaa cgccaataac
     3001 tgtatcagct gctcaaacta ttcaacctgc atttgatatg acattattag ctagttatta
     3061 tgcactattt gtatctgcgt ttatagtagc tacagtatca acaatagtaa cggttaaatt
     3121 tatagaacct aaattaggta agtatactgg taaaccagaa ggtatagatg ataatcaagg
     3181 ttttgaggtc acaaaagaac aagcaaaagc agcaaagtta gcaggtttta cagttttagc
     3241 ttatgtagcg ttattaattg ctttgtgtat accagctaat agttttttaa gaagtccaga
     3301 aggttcttta atatttggag caccacttat gtcttgctta cagtttttta tagtaatact
     3361 attcttcttg ccagggattg tgtatggaat gagggttggt aaaatcaaga ctgtaaaaga
     3421 cttaaatgat atattatgtc aatcaatggc agcaatggca ccgtttatcg tacttgcaat
     3481 agttataggt caattcttaa cgctattctc agtgtctaat ttagcacagg tactagctat
     3541 aaaaggtgga gcattactca actcattgcc actaccacca caagctataa tactgctatt
     3601 cttagtacta gtagcgttta taaatatatt tgttataagc ggaagtacaa aatggatgat
     3661 atttggacca gtatttgttc caatgttaat gcaattaaac atacatccag catttactca
     3721 gtttgtatat agattaggag attctgtaac caatcattta tcaccattaa atgcattctt
     3781 tataatactt ttagcaacag tccaaaaata cgataaaaat gcaggtatgg gaactatatt
     3841 ctcagctatg ataccttaca ctataggtta ctttgtagta cttgcagttt tagtagtagt
     3901 ttggatgaca ataggtatac cagtaggaat aggtggacaa gtttggttat aaaacatatt
     3961 taataatttt gggaggaaag aaaatggaaa caactgaaat aagatacaat tttgatgaag
     4021 taatagatag aagtggaaca aactgtctta aatatgatct taagagtcaa tttatgccag
     4081 aatctccaga agatgcacta ccactatggg tagcagatat ggattttgca tgtgcccctg
     4141 agataattaa tgcaatgcat gaaagaatag acagaaaaat atttggatat agctctcaac
     4201 aaactgatga ttattatgaa gctgtatgtg gatggtttaa gagaagattt gattggaaaa
     4261 tagaaaaaga gaatatattc tttagcccag gggttgtgcc tgcgttagga tttttcacac
     4321 aaatattaac acaaaagggt gatggtatca taatagaaaa gcctgtatac tatccttttg
     4381 aagataaaat aactaataat gaaagaaaag tagttaatag tcctttaaag tttgaagatg
     4441 gtcattattc tatggattat gatggatttg aagaattagc taaagatgaa aataataagg
     4501 tgtttatatt atgtagtcct cataatccag ttggtagagt atggaaaaaa gaagagctta
     4561 gaaaaatagt agacatatgt aagaaatatg atttgtggat aatatctgat gaaatacatt
     4621 gtgatattat tcgtaaaggt gaaaagcata taccattaga agtagcatgt cctgattata
     4681 aagatagaat aataacttgt acagctccaa gtaagagttt taacttagct ggtatgcaat
     4741 tatcaaatat agtaatcaat aataaggaat tacaaaaaaa atggatgctt gaaacagatt
     4801 ctaaattagc tatattccta cctaatccat tcgcaatagt tgctactaaa gttgcttata
     4861 atgattgtga agattggatg aatcaagtaa atgagtatat agatggtaat attaaatatg
     4921 tagaagattt tgttaatgag tatatgccta aagtaaagat aataaaatct caaggaactt
     4981 acttagtttg gttagacttt agagggtatg gattagatgc aaaacagctt gaatatgtaa
     5041 tgcttaacaa ggcgaaggta ttgctagatg aaggatacat attcggagaa gaaggagcag
     5101 gatttgaaag aataaatgtt gcttcaccta gaacaattat agaagagtgt atgaatagaa
     5161 taaaatgtgc atttgaagga ttataatagt aatatctaaa taatatattt gtgtatttta
     5221 aaaaaagact atgctaaaaa aatatttcaa agcatagtct tttaaagttt tatgatgagt
     5281 ttaaaaaatt tcaaaacgtt actattcact tatatgttta tattagaaat aattccacaa
     5341 tatactcatt ttctaaagtt gcacttttat acaccttgct tcaccaatat catgtgtaac
     5401 caaaatagta gacttattct cattcttaat aatactataa acatcatcac aaactaataa
     5461 tctagtctga taatcaagag ctgaaaaagg ttcatctaaa agtagaatat ctggatttac
     5521 agaaagtgtt ctaataagcg caactctttg tctcatacct ccagataatt cttttggata
     5581 catacttcta aaatcccata atccataagt ctttaaaagg ctttcaactc tatctaaaga
     5641 ttttttatct tttttgcctt gaatttccaa tccaatagtt atattatcca ttatatttct
     5701 ccattcaagc aaattatctt tttgaaacat atatccaata tttccagtaa tctttatatc
     5761 accactagtc ggttttaaaa gattagtaag aatatttaat attgtagatt taccacttcc
     5821 agaaggacca agcaaagtta atatttctcc ttctttcaag ttaaaattga catcctttaa
     5881 tacttcaagc tcgccttcct tagtgtagaa gttcatattt atatttttaa cttctactat
     5941 gttgtcattc attatgccac cccctataac ttttagctcg tatatattat gttattaaca
     6001 atatgaccta tttgtaccat ttttcataaa tataatataa atattttgtt tatttacgta
     6061 ttttcttagt aacttgtacg tataaattat agataaataa attataaggt aggggataaa
     6121 tttgagtaac ataataacga atttaataga gtcaatattt actaatccaa taactatatt
     6181 gtttttactt gcaataactg tgtattctgt ttttaaaata atagaaaata acagagtata
     6241 ttcatttgta agttcaaaat tagacgaaat caatagagac tataagacaa gtgagtttta
     6301 taaaaaggta agggatgact actatacata tagtaaagaa aatccttatg cagacgtaaa
     6361 tataacttca tttatagagg aagttgtatc tgatttaaag cataataatt tgccattgtt
     6421 agaaaaaatt agaagtataa aaaattcatc ttcaataagt atattacttg gggttttagg
     6481 tacgtttgtt ggtctttcta caatgctttt atgtgttgat actaaagaca ttataaattc
     6541 acttccttca actattagta gtatgcaaac tgcatttaca actagtatat ttggagttgt
     6601 attttctcta attataggtt actttactaa gataaaagat tgtgaacatg ttttgataca
     6661 aataatgtta aaatctgaaa atttactaac atctgaaata actcatttta agtcagaaag
     6721 aatggattta aaagtagaag aggtaaaaaa tacaataaaa caaataagta agtctataga
     6781 agctattgaa aaatttgata aaatatctaa agatttaaat gactttaatg atgaatttat
     6841 cagtggtata gaagctttaa aaagcctttt agaaggttct caaagttcta ttaagacatt
     6901 tgaccagagc gtaagaaaat tagataaaca gtttaatatt ttaaatataa aatttgtaaa
     6961 gctttttgat aaatatgata atcaagataa tataaataaa gagattttat ttgatataaa
     7021 agaatcttca aaaaatatat acaatgccac tgaaagccaa ttcaaaatta gagattatat
     7081 aaaaaatata aatgcaggat ttgctctata tgaaagaagt gctcaagatt tattaactaa
     7141 attaatgacc catgaaaata aaatatctca aaatcaaaaa atccttttag atgaaaagtt
     7201 taccttagat gactcaataa aaaatctctc aagtattatt gaaaactttt ctaatgacct
     7261 acaagttaaa ctggatatga tgtttgaaaa ttctttagat attcaagata agttagatgt
     7321 tatgtttaat aactctttta tgaatgatga agttcctctt gatagtgaag aattatttaa
     7381 ggatgatgtt aataatgtat ttaatccttt tagtgaagaa atttatgaga tagaggataa
     7441 agaaataaag gtcataggtg aggatgagtt aaatgaataa taaatataga agaactgtta
     7501 acagagaatt tgaaaagagt agcttttggc caacatttac agatttatta tctactgtat
     7561 tgatggttgt gattcttatc ttatttagct cagaaagtat ttcaggttct gttgaacaag
     7621 atttagctaa gaatgtcaat gcttctgttg aagaaacttt taaaaaaagt ggaatacctg
     7681 tgaaggtaga taaatctaat gggcaagtaa cttttggtga aaaaaccatg tttgatgtag
     7741 atagtgatgt tttaaaacca gaggctaaag aaatgctaaa aatgtttgtt ccaaagtaca
     7801 tagagactat ttataaagat tatggtgact atatatccaa aatagttata gaaggtcata
     7861 cagatgatgt gggaagttat atatacaact tagatttatc gcaaagaaga gcatatagcg
     7921 ttgctaaatt tatagtggga gatgaaatag gtgactataa atacaaagat aaagtgacta
     7981 agcatattat tgcaatagga aggtcaaaag ctgaactcat caaaaatggt gacaatagcg
     8041 tgaatagaga tgcatctaga agagtagaac taaaatacga aataaatatt aatcaaaata
     8101 aataattaaa tttttataaa aaaaaagaag gcagttggtg tgagctttaa gccttcttta
     8161 aggaaaaagt ttatttattt ttgttgatac ttaacatatt atagttttta gaagcagttt
     8221 gcaatatatt aaacgtaaat ggtcgtttaa aatgcataac tggatactac tgatgtaaaa
     8281 tatttcattt tatataatat aaaaattata cagtgtcaat tttctatatt attgtactat
     8341 aatctattat ttaaaatatt atgtaaaaaa atatcataaa aacgcatatc atgttataat
     8401 tactagagta ttatgaatat ggaggcgtag tttgtgatag aaataagtat aaaaaataaa
     8461 aaaaattcta aaattaaaac tttgatactt ttattgatag tatctatatc gatttattta
     8521 actggttgta ctacatcaga taatggaaat aatcaatcaa ataataatga ttctttgtct
     8581 agtagtatat caagtgataa gaatgtaaaa attcatttta ttgacactgg aaatagtgat
     8641 gcaatattga ttcaagatgg taaaactttt acattgatag atgggggaga taatgatgat
     8701 gagggattaa tggtagatta cctaaacaat caaggtgtaa aagatattaa atatcttata
     8761 gctacccatt ctcatgcaga ccatttaggt ggacttgatt ctgttgtcaa aaactttaat
     8821 atagaaaatg tttttgtatc gaatggttca gcagaaacaa agagttatag ggactttata
     8881 aatgctttag caaataaggg tttatcccca agtgtaccgc ttgaaaataa taagttctat
     8941 ttagaagatt cttattttga agttctaaat acaaatgggg gagatactac taatgaacaa
     9001 tctctagtcc ttgtatatac taatgggaat gataaagttt tatttacagg tgatgctgaa
     9061 gaaggtaccg aaaaagaaat actacctaag cttgagaaag tagacctttt aaaagtagct
     9121 catcatggtt ctcgtagtag ttcatctcaa gagtttttag ataaagtaaa tcctgaatat
     9181 gcggttcttt tagtaggcaa gggtaattca tatggtcatc cacatcagga gacaatgaat
     9241 aaattaaaaa aaatgggagt aaaagtgcat agaagtgatg agtgttcaga tataatattt
     9301 gaatcaacag gtgatggagt atttacaagt tgtaaagatg gaagttataa taaaggcgtt
     9361 agagaagatg gaaactctgg aaaatcaaac agtaatacaa gtaaaaatga ttcttttaaa
     9421 aataaacaaa atacatccaa ttcagaagaa atagtgtatt ttactccaaa aggtaagagc
     9481 tatcactcta caaaaaattg tagtgggctt tcaagaagta aaaaaatatt atcaggcaca
     9541 attgcagaaa gtaagaaaaa tgacccatgt gatatatgtt atggaaaata acaaaaaaac
     9601 aaccaattta tctataaagc ttctgaactt taaatcattc aaaaaataaa ataaatgcat
     9661 aaaaaaataa gctataatca atagataaca cattttatct atacgaatag cttatttttt
     9721 taagtatttt ttaatagcta tttaataact ctaaatacac cagttacaat acctacaatt
     9781 tcaacttctg agtcattgag cataattggt tccataaaat cattttctgg ctgaagtctt
     9841 attaaatttc cttctttaaa aaacctctta actgtagcag attcaccatt tataagtgca
     9901 actactatct gagaatttaa ggctgtattt tttttgtcta ctacaacgta atcttcatct
     9961 aaaattcctg cattaatcat actttcgcct tttactctta atacaaaatt gttactgccc
    10021 tttactaaac tggcagggaa tggaatgtat tcctctatgt tttgttgagc taaaatgggt
    10081 tctccagctg ttatttgacc tactaaagga agctctatta tttcttgatt agctccagaa
    10141 acatcattta ctttactacg ttctaaaact tcaatagctc ttggtttagt agggtccttt
    10201 cttatgtatc ccagtttttc aagtttattt agatgagagt gcacagttga agtagatctt
    10261 aaccctacag cagtacatat ttctcttaca gcaggtggat aaccttttaa tatgatttga
    10321 gactttataa attccaatat caagacttgc ttttcagtta gatctaaata cataacatca
    10381 ctcatttcca aacatatttt gtttttattt ccatacactt tatattttta attataacat
    10441 aatccattta tatctgcaaa catttgttcc tgatttttaa tcgtcctcaa tccattcaaa
    10501 attcttgact atatcacgtt ttacattttt cttatgagct gcataaagct gagtagttgt
    10561 gacattatca tggtcaagta agttttgaac atcatatata gaaaatcctg tttggattag
    10621 agaagttgca gccgttgctc ttaatttatg gggactatat ccattgtctc tagtagtatc
    10681 aagtggaata gatgtatatt tttttaccag tgttcttata gcctttgctg taattctctt
    10741 attttgaaga gacaaaaata aagcatcttt ttcttcatca tttaatagtt catctcttgt
    10801 tctttcgttt tgcaaataat cttttattac atgttcacaa gtatgattta taggcattaa
    10861 aacttctttc ccacgttttc tatatatttt aaattcacct cttgaaaaat taaaagagga
    10921 gatattgagt tctctaagct catttaatct aagcccgtat gtaacaaata atgctagtat
    10981 agctttgtca cgtaatttag tttttctcca atataccttt tctttctctg taagcccttc
    11041 acctgtttct actgattcaa gcatcttagc aacttcatct atctcaaggc gcttaatagc
    11101 atctggctga ggtttaggaa gttttattgg attaaatcca tcagttatat tattatctat
    11161 ctgagagttt ctgtataaga atttaaatag agtagagatg gatgattttt ttcttgctaa
    11221 agctctattg ttattttcaa aaatcaatgt gtttttttct gtatttttgt aatatctact
    11281 gcaataatca cctaagaata aatttatatc tcttgatttt attgtattaa aatcatcttc
    11341 tgtaatatct tttacacaat ctgcatttgt taattctttg gtttcaatca aatatgaaca
    11401 aaaaaaatta atatcttcta aataagcaag tcgtgtagaa actgcaacag aaccttttaa
    11461 atatataaaa taatccttca taaatacagg aagtttattt tccaaaagta gacatttttc
    11521 tatgagttca ttttctttga taacaatatt atcaggttta tctgatttat tatgtacttt
    11581 tataattttc ttatcattat tcaaaattaa
//
LOCUS       DAELNY010000119        11393 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.119, whole genome shotgun sequence.
ACCESSION   DAELNY010000119 DAELNY010000000
VERSION     DAELNY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.119"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            301..1569
                     /locus_tag="KPE55_002605"
     CDS             301..1569
                     /locus_tag="KPE55_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0493659.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            1764..2552
                     /locus_tag="KPE55_002606"
     CDS             1764..2552
                     /locus_tag="KPE55_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0493660.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            2552..3001
                     /locus_tag="KPE55_002607"
     CDS             2552..3001
                     /locus_tag="KPE55_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0493661.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            3005..5269
                     /locus_tag="KPE55_002608"
     CDS             3005..5269
                     /locus_tag="KPE55_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0493662.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMSLFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            5409..5720
                     /locus_tag="KPE55_002609"
     CDS             5409..5720
                     /locus_tag="KPE55_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493663.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            6006..6506
                     /locus_tag="KPE55_002610"
     CDS             6006..6506
                     /locus_tag="KPE55_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG0493664.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            6563..7549
                     /locus_tag="KPE55_002611"
     CDS             6563..7549
                     /locus_tag="KPE55_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0493665.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            7599..8321
                     /locus_tag="KPE55_002612"
     CDS             7599..8321
                     /locus_tag="KPE55_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG0493666.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            8350..10284
                     /locus_tag="KPE55_002613"
     CDS             8350..10284
                     /locus_tag="KPE55_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0493667.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            10277..>11393
                     /locus_tag="KPE55_002614"
     CDS             10277..>11393
                     /locus_tag="KPE55_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG0493668.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILL"
BASE COUNT         4559 a         1040 c         2059 g         3735 t
ORIGIN      
        1 atgaaatgtt ttattgttaa atttaaactt attttaatgg aggaaaaagt cgaaaaatat
       61 ctaaaaagat atggatataa aaaatattaa tatatatata ctataaactt gacaaaatca
      121 accttttaat aactagcaaa aaatagcaaa attatataag cgttatttaa ttgtcaaaaa
      181 tgaaagctaa attgcatgtc ctaaaaaaaa tgattgataa catttatttg tgtgctataa
      241 ttatatagta aaacaataag tatattgcat aatgtatgca aatcgggagg agagacggac
      301 atggaagcat ctatacacac aattgccagc aataatttat tgataatttt tgctattgta
      361 agtataactg gaataatttg tagtaagctt agtgaaataa taaaggttcc agatgtagtg
      421 ttatatttac ttgtaggtat tttaatagga ccatcatttt taaaatttat agatattcgt
      481 ggatttcaaa tagaaaatca actaatactt acatttggtt ctgcatttat tttatattta
      541 ggtggaaaag aaataagttt aaaagtattg agaaatgtga agatatctgt attcttatta
      601 gctactttag gagttgtgat atctgctttt ataatgcaac aagtaatagg atttactttt
      661 gggataagtg caatgactgc tttactagca ggaactataa tagcatcaac agacccagcc
      721 actcttgtac ctatatttaa tcaggtaaag ataaaggata gggtaaaaca aactgtaata
      781 agtgaatcag cttttaatga tgcaacagga gctatactaa catcagcagt gttggctgta
      841 attttgtcaa ataaattttc attaggtgaa aatatttatg aactggggac aatggttatt
      901 gtcggagttt tagttggttt aattacagga atactgcttt tgaaattagt taatgataaa
      961 ccttatggaa tatttaaaga ttttgcacca ataatatctg ttatttcagt tgtagttgca
     1021 tatgaattat ctacaaaatt aggtggaagt ggatatatgt cttgctttat tgtaggtata
     1081 ataactggta acaagaaaaa ctttaaaatt tggttaagtc aaaaatctta tgatgcagat
     1141 ttttatgttg ctgaaacttt aggtactatt tgtcgtatgg caatatttat aatattgggt
     1201 agccaagtta atttggctca gttaagtaaa tattttatgc catctgtaat tacagtatta
     1261 gtattaatgt ttatagcaag accactttgc gttttagttt gtacacttgt agacagggaa
     1321 gcaaaatgga gtaaaagtga aatattattt atgatgtggg taagggaaac aggcgtaata
     1381 ccagcagcac tttgtggtat tatttcagca atgaaagttc ctggatacga agtgatttca
     1441 tctgtagtat tcatgactat actaattaca ttagtaatac aaggaagtac tacaaaatta
     1501 gtagctaaaa aacttggatt attagaagaa gaagtgataa atataagtga aaaagtatca
     1561 actttttaaa gttttgcttt aaaatagtac ctgttaatgg ctattttaat attgtctaat
     1621 gatgattatt gaaatagtca tttttttatg tataagtaat ttacagaatt tacatattag
     1681 acatatttat tatcaaaatt tgttgaagct atgatataat tagttgtatt taaaataaga
     1741 atagcaaata agggaggaaa tttatgttta cggttatgga tattgtacag ccagatacag
     1801 tagaagaagc gtactcaatt ttaaataaaa gaaaaactaa ccaagtaatt ggtggaagtg
     1861 cttttttaag aatggggaaa aaaagaatag gaacaggaat agaattatct aaattaaatt
     1921 tagattatat aaaagagtat gaagattacg ttgagattgg ttctatgact acttttagaa
     1981 cattagaaac aagttccata ataaaaaata attttggaag aataatagaa gattcagtta
     2041 aaaatataat aggagttcag tttagaaatg tagtaacagt tggtgcaaca gtattttcta
     2101 aatatggttt ttctgacttg atagtagcat tactttcatt agatacagaa gtagaattat
     2161 ataatattgg gagaattagt cttgaagagt ttctaaatag agattatgag aaagatttac
     2221 ttattaagat atacataaag aagacaaata agaatgcttc ttataaatca cttagaaatg
     2281 caaagagtga ttatccaatt ctaaatgtat ctgtatcaaa acatatggaa caatttaagt
     2341 tatgtgtagg agcaagaccg cagaaagcta cgattgcaaa acaagctagt gaatttctat
     2401 ctaacaatga aatcaatgaa attaatatag ataaagcagt agaaatagca tcagaagaac
     2461 taacttttgg ttctaacatg agagcatcta gggagtatag aaaagctatg agtaaagtac
     2521 ttttaaaaag agctataatg gaggttattt aatgttagta aagattaata taaatgggaa
     2581 gaaaaaaagt gttgatatag aaccagaaga atatctggtt gatactttaa gaaaagttgg
     2641 taatttaagt gtgaaaagag gttgtgacac aggctgttgt ggtttatgta cagttttaat
     2701 tgacaagaaa cctacattat cctgtgcaac actgacattg agagctttag ataaagaaat
     2761 aactacaatt gaaggtttag aaaatgaagt taaagaattt agtgaagttt tggtaaaaga
     2821 aggagctgag caatgtggtt tctgttctcc tggctttata cttactgtac ttgcaatgaa
     2881 ggatgagctt gaaaatccta cagatgatga aataaagcat tatcttacag gaaatctatg
     2941 tagatgtaca gggtatatgg gacagttaag agctatcaaa acttatctgg aggtaaaata
     3001 gaacatgaat atagttggaa aaggaatcaa gaaaatagat ggaatgagta tcacaacagg
     3061 aaagcctgta tatacagatg atttatcagc aaaagatgct ttggtggtaa agatacttag
     3121 aagtcctcac gcttatgcaa aaataaaaga tatagataca tcaaaggcag aaatggttga
     3181 tggtgttgaa tgtgttttaa catataaaga tgtaccaaaa tctcgattta cacttgctgg
     3241 tcaaacatat ccagagccat ctccatatga tagattaatt cttgaagatg ttgtaagata
     3301 tgtaggtgac gaagtagcta tagtggcagc tatagatgaa aaaactgcaa ttaaagcaat
     3361 gaatcttatc aaagtaaagt atgaagtact tacacctgtt ttggattttg aagaggcaat
     3421 aggaaataaa actatggtac atgatgaaga tagacattgt aactttgata taggtatgca
     3481 aagagaaaaa aatatagtat cccatcatga ttatacaaaa ggtgatgtag aaaaagtatt
     3541 aagtaattgt gatgtagtga ttgaagaaac ttattatact caagctcaag cacatgcaat
     3601 gatggagacg tatagggcat acaattattt agaccatatg ggaagattgg ttgtagtaag
     3661 ttcaactcaa ataccatttc atgtaaaaag acatttatct cgtgcactag aaataccagc
     3721 tagcaaaatt agggttataa aacctagaat tggtggcgga tttggtggta aacaaactgc
     3781 atgtgttgag attttttctg caattgttac tttaaaaaca gggaaaccag ctaaaataat
     3841 ttatgataga aaagaaactt ttaattgttc aacaagtaga catgcaatga gattaaaagt
     3901 tagactaggt gctacaaatg atggcataat aaaggcaata gatataaaag cactttctga
     3961 tacaggagca tatggagagc atgcttctac aactattggt ttggttggag aaaaaacttt
     4021 accaatgtat aataaagttg aagcaacaag atttattgca gatgtggtat atactaataa
     4081 gatgccagca ggagcactta gagggtatgg agcatcacaa gggtgttttg ctgttgaatc
     4141 aactataaat atgcttgcta ataaattaaa aatggaccca acagatttaa ggttaaaaaa
     4201 tgtggtaaca gaaggtgaaa ctacatttgc atatggtaaa actttaaata gtgtcgattt
     4261 aataaaatgt ataaataaag gcaaagaact tattggatgg aatgaaaaat atccttgtaa
     4321 acatcttgat aatggaaaaa ttagaagtgt gggtatggca attactatgc aaggttctgg
     4381 tatacaaggt cttgataagt cttcagctga aataaaattg aatgactgtg gagattatac
     4441 tcttatgata ggttctactg atatgggtac aggaagtgat acgattttag ctcaaatggc
     4501 tgctgaggtt cttgatacaa gcattgaaaa tataacagtt atatcagctg atactgactt
     4561 agttccatat gacccaggtt catatgcttc aagtactact tatgtaacag gtatggcagt
     4621 agttaaggca gctcaagact taagagaaaa aataatttct gctggagcaa ctaaactagg
     4681 tgttgagaag gatgaagtag aatttgatgg tataaaatta actagtaaaa aaggtagcac
     4741 tttattattc gatatagcag ttgattctac aattggtcca gatactaatc aattaatagg
     4801 tcttgcttct catggtagcc cagtatctcc tccacctttt atagcaggtt ttgcagaaac
     4861 tgaaattgac acagaaactg gaaaagttga tttagtaaat tatgtagctg tagtagattg
     4921 tggaacggtt ataaataaaa atctagctaa gatacaagtt gaaggtggaa ttgttcaagg
     4981 gattggaatg tcattatttg aagaagttag atacacagaa aaaggaagca tggacacaaa
     5041 tacatttatg caatacaaga ttccagctag atgtgatatt ggaaatgtta aagtagattt
     5101 tgttgaaact tatgaaccaa ctgggccatt tggtgctaaa tctgttggag aagttgttat
     5161 aaatacacct ttaccagcaa tacaagaagc tgtgtttaat ggaattggtg taagaataaa
     5221 tacactccct attacacctg aaaaagtttt tatggaaatg aataaataat tatttatgaa
     5281 aatttagact agttttacat gttaaacaac cagttatgtc atacctgttg aaagatattt
     5341 aataagtagt tattataata ttatgaattt tacttataaa atacaaaatt aaataagggg
     5401 atgatgtttt ggctggtaat ctaaataata tgagagcagt aaataatttt agaggagata
     5461 agaacatttt agaatgttta gtcagctttg agggtcgttc aataagtcag agaaaagtaa
     5521 gggtattttt taaagaaaaa caaaatcaaa tagaaattga ttttgcagaa gaggaaattt
     5581 ctaaattggt tgaaaatgtt gttttaaata catcatatca agaaatgtta tatgatgaaa
     5641 tagagaaaca actggaaatt gattgtatag gtacttggat gatattatct aaattaaaag
     5701 atggtagtag agttcactga ttttaaaata aattattaga gcctaatttt aaggctcttt
     5761 tttgctgttt ttaattaaca atagaaaatt tatctttagt ttgtatataa ttttaatatc
     5821 atatattcta tatatataaa attgaatttg tatgtattat attgtataat ataaaatgaa
     5881 ttgggaaaat agttcgctaa aataatttgt ttttataaaa ttactgttta agtttataga
     5941 ttttgtataa aataaagtta tgttattaat ttttttaaaa ataaaacttt tactaggaga
     6001 cagatatgag aaaaaatagt attaatatag cactaactgc agcaattaca gcaatctatg
     6061 cagttttaac attatcattg ggatttataa gttatggacc aatacaattt agggtagcag
     6121 agattatgat gcttttggca tttttagata aaggatatat agtaggtctt acattaggtt
     6181 gttttttagc aaatgtaata ggaccatatg gtgtacctga tataattttt ggaacattag
     6241 ccacatttat aagtgcaagt atgatttata ttactagaaa gttaggtaaa cagaataaaa
     6301 gtactttagt tatagcttct ttatggccaa ctgttataaa ttcgattgtg gttggtctaa
     6361 tgttaaatgt attctttggg cttccattaa ttctatctat gatacaggta ggttttggag
     6421 aatttgtagt agtaacaatt gtaggagtac catttttctg ttttttaatg aaaaaatata
     6481 aaaatgtcat taatattaaa ttttaaatag tgtatagtaa tttaattaat tgagagtatt
     6541 attagtgaaa agaggtgttt atttgtcgaa ggtaaaaaga ttgactgatg tttggatata
     6601 tattaatgat aagcaaaagt ttacaataaa agagttgtca gaggaattta atctttcagc
     6661 caaaacaata caacgatatt taaaagaatt aaataaaatg ggacttccaa ttcaagcaga
     6721 acaaggtaga aatggaggtt atagggtgct aaataatagc tatatacctc ctgttatatt
     6781 tacagaaaaa gaagttatga ttatactatt tgcacttaag tcacttcaga tttataatta
     6841 taagttaatg gaaatagaaa ttaattctat aatgagaaaa gtaagttatg aacgtaaaag
     6901 cagtataaaa gtaggtatag aaaatattaa aagatacatt ggctttgtag ctaatgatac
     6961 taatgatgga gattttaaga gtagtaatgt aactgagttt tttagagctt cttcagaatc
     7021 tctaatttta aatattaagt ataagtgtgg taatcagatt ttggaaaaaa acgtatgccc
     7081 tataggtata tatttaaata agggagcatg gttttcacca gcatatgatt gtaaaagtga
     7141 taaaataata ttagttcata tagataatat aattgatata aaaagaacag gagaaagtaa
     7201 aaaaatacat ataaacttgg aagaatggtt acatgaagta tataacaatt tatatggaaa
     7261 tagttcaact actaaaaaat ttggtgaaat tatttatcta aatgtattat taactaaaga
     7321 aggtgtttct aaaataaagg acagttcttg gaatttagat atggagctaa atgaagatgg
     7381 tagtggaata attagtacat tcataaaggt tgaagatata gattgttatg cttcgatttt
     7441 attttttatt ggaaataatg ctaaggttat agagccaaaa tttttgaata agctacttta
     7501 tgataaggcc aatgagttaa gatattttta tgaagaaaat atggtataat atttagaaat
     7561 gttgaaatta aataattatt aaggagatat acgttataat ggaaaatttt aataagaatg
     7621 ctaaaataat tggtattgga gctgaaggta ttaatataat aaatgaagta gaagaaaaaa
     7681 taaaagcaaa tatggatata gaaaaaataa acataaatca agagattgaa aaagaatatg
     7741 ttagaagtct tttagatggt gtagatatat tatttttgat atattcatct gaagataaac
     7801 atattagaga tattataaaa gctgtttcat atatgtcaaa cgaaagaaga gttctatcta
     7861 taggaatgga ttgttcagag aaagaaaata aagaagattt agagcttggg agagagttta
     7921 aaatcaataa tgatagtata tttaaatttg ttgatttaat gaatatcatg gtagagtcta
     7981 tttctgattc ttgcatgata aatatagata ttactgattt aaaagaagct attgtaggag
     8041 ataaaggaat taagtattct tttgaagagt ttgaagatac aaagtcatat agcgagatag
     8101 ctgacatttt atttgacagg atggaatata ttggtgagga atttataagt aagaaaggaa
     8161 ttgtatttgt tgaaggaagt ccagaatttt ctataatgga attgaatgat ttaataagta
     8221 atattcagag caaggtagaa gaaagctatg aagtaatatt ttctttatat ataaaggaaa
     8281 atttaaatgg aaatattaga gtaggcttat tgtataatta gtatatttta gatattaagg
     8341 agagtgtata tgtctgaaaa tataagaggt acagagtatt ggttaatgga agaatttttt
     8401 tatcaggaaa cagacacacc acagattttt agagaagaaa gcataattgc acttggattt
     8461 gatataaaaa taaatttaaa aaaagtattc agtatgggga aaaaatcaga aaagtatatt
     8521 aatgagaatt gtaatgataa agattttata aaaaagttta tgtcgattaa aaaaggagat
     8581 tattttttat ataaagcata tcaaaatata gaagaaaaag ttaaatgtaa tgtgtctatt
     8641 ggcatagttg aagaagattt tgaaaatgga tatgaattaa gtcatatttt agggcataca
     8701 ctaccagtaa aatggatgca taattttgat acagtttcaa attgtaaagt atatacaagt
     8761 gaattttata aattaaataa taatgatatt agctcatatt tagaaattat taagtcacaa
     8821 gataagaaat tattgagaaa aagaatagaa gaaagtgaaa ataataaatg gtatttaaaa
     8881 aactattatt ataaaaatgt agataagtgt gtagatgatg aagaattatt tattcagccc
     8941 attaatctag aacaattaga aagtctaaaa aatataaaga cagatgatta tattttattg
     9001 tataatagcg agaaagtaat ttgtgaaaat gaccatacaa tgaagagtct atttggcgta
     9061 tgtcaagtta tggaagggtt cgataaagag aaagtaagtg ctttggaggg tggatatttt
     9121 tttcctgtta gatggataga gtatgaaaag gagaatatag acggaaatgc atgggttata
     9181 tttgaaaata atagtatagt tgaaaagtat gtaaaaagat ttactaataa aatgccatta
     9241 aatactatac tttatggacc accaggaact ggtaaaacat acaatacaaa acagatggtt
     9301 tacaatctag taagtgaaac cagtctaaga aacagatact atacaggtta cttagattat
     9361 aaaaacaatg tagaattttg tacatttcat caatcatatg gatatgagga atttatagag
     9421 ggtcttaagt ctgatggtga aggcaatttt aagccagaag atgggatatt aaaggatata
     9481 tctatagaag catgttataa tgcattgaaa tttgaaaaaa agatgagctt tgagttggaa
     9541 aaagatagct tgactttaga aactataaaa gacaggaaaa agaaattagt attagaaaat
     9601 atagaatata gtgagagttt taattttgaa gaatcagaaa actatgtttt aataatagat
     9661 gaaattaata gagggaatgt atctaaaata tttggtgagc taataacatt attggaagat
     9721 gataaaagac ttactaaatc aaatcaaact attgtcaaac taccatattc aaaggaaaaa
     9781 ttttctctac ctccaaattt gtatatagtt ggaactatga acacatcaga taaatcaata
     9841 gcacttttag atatagcttt aagaagaagg tttaattttg aagaaattat gccagattat
     9901 gatttattgt ctattgtaga tggtatagat attagaaaaa tgctatatac aatcaataaa
     9961 aggatagagt ttttatatga tagagaccat gttattggac aagcttatct acttgctgta
    10021 gaaaatatta atgatatagt agtagtattt agaagtaaaa taataccact tcttcaagag
    10081 tatttttatt atgattcaga aaaagtaggt ttgattttag gaggaattgg aagaaatgaa
    10141 aaagataaat acattgttta taaagaaaaa cttatagctt ctgagttatt taaaaataat
    10201 aatgcctcaa taatggagag taaagttaac tattgtataa aacaaaacat atctagtgag
    10261 gagctaaaaa atatatatga gtaaaaatat atatgttaag gaaacctatg aatggataag
    10321 gataggtaat ggtgaaaatg agcttacaga aattgaatat gaaaaattgc ttaagtatct
    10381 agagaacaat aatgatgtat taaaaagtaa tataatagat attaaataca aaaaacttag
    10441 atttataaac tatgtaggca tcatatgttt tgaaaatgta atacttgaaa tacttcctaa
    10501 attgtcatta agtgataatc ttgtaaaaga tagagagata ctattacaga tgctttctat
    10561 atgtaataaa attccaatta caatgaatga aaaaataaga ttgagtttga aaaattataa
    10621 tctcttaaat ttttttgtta tgtatttcat tgagagtatg caaacacaga tgaaaaaagg
    10681 tatttatttt gaatatataa ataaaataga gaacttaaat gttatgaggg gtaaaatatt
    10741 attaagtaca tatgcaaaag aaaaaggcat ttctccaatg aaaataagat gtgaatatga
    10801 tgagtatagt gaaaataatt tcttgaatca agttcttaaa aaagcatgta tatctatttt
    10861 atgtagaata aatgacaatt caattcaagg caagattaaa aaaattttaa gctattttca
    10921 aaatgtagat ttaatttata tagatagaaa aaaactattg gattataaat tttataaaaa
    10981 caatgataga tttaaagatt gttatttact agctagactt ctacttttaa atttgtctat
    11041 ggataatagt caaaataatc aagaggcatt ttctatatta tttgaaataa atactttgta
    11101 tgaagaatat ataggaattt taatcaaaag tatatgggac aattcattta gagaaacata
    11161 tatacaagat aagagtaaat ttcttttgaa aaatgaacaa acaggtaaga aaaactttaa
    11221 tttaagaccg gatatagttt tatatgactt aaaaaatgaa tatgaaatta taattgacac
    11281 taaatggaaa gctattgaag tagattcaaa tgttttttat aggtcaagtg atatatacca
    11341 gatgtatgca tatataacag catatgaaaa tgcaaaaaga tgtatattat tat
//
LOCUS       DAELNY010000120        11156 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.120, whole genome shotgun sequence.
ACCESSION   DAELNY010000120 DAELNY010000000
VERSION     DAELNY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.120"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            249..695
                     /locus_tag="KPE55_002615"
     CDS             249..695
                     /locus_tag="KPE55_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493669.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMQIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            1076..1993
                     /gene="pstC"
                     /locus_tag="KPE55_002616"
     CDS             1076..1993
                     /gene="pstC"
                     /locus_tag="KPE55_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG0493670.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1995..2819
                     /gene="pstA"
                     /locus_tag="KPE55_002617"
     CDS             1995..2819
                     /gene="pstA"
                     /locus_tag="KPE55_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG0493671.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2838..3602
                     /gene="pstB"
                     /locus_tag="KPE55_002618"
     CDS             2838..3602
                     /gene="pstB"
                     /locus_tag="KPE55_002618"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG0493672.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3630..4283
                     /gene="phoU"
                     /locus_tag="KPE55_002619"
     CDS             3630..4283
                     /gene="phoU"
                     /locus_tag="KPE55_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG0493673.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4441..5310
                     /locus_tag="KPE55_002620"
     CDS             4441..5310
                     /locus_tag="KPE55_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0493674.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5397..6833
                     /locus_tag="KPE55_002621"
     CDS             5397..6833
                     /locus_tag="KPE55_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG0493675.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6881..7708
                     /locus_tag="KPE55_002622"
     CDS             6881..7708
                     /locus_tag="KPE55_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0493676.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    7999..8261
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8337..11003
                     /locus_tag="KPE55_002623"
     CDS             8337..11003
                     /locus_tag="KPE55_002623"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG0493677.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         4405 a         1195 c         2084 g         3472 t
ORIGIN      
        1 taaataataa gattaagaat ttcttaataa atccagaaga tgctttcgat tatataatat
       61 tttatatttt tatattatta attatatatg ttgcaattgc tgcacctact ttttctggtg
      121 agtatcagac acaatcagat agcatccttc gttgtacgaa acatggacat attcgtctag
      181 cgtttactaa aatattagct ctattttcta tatttataat aatgtttgtt atatgtattt
      241 ctatccattt ggcaatttct gatttagctt ttggtactga atgtttgaaa acctcatttc
      301 aaatgttgtt ttctgtcatt agtctagtaa atataaattt actacaagca cagattctta
      361 tagctattgg tggtctaatt tcaatacttg caatggttag tttaacatta tttttatctg
      421 caaaatgtaa ggattctctt acttctatgc agattgcttt ttctatttgc ttaattccta
      481 tgggaatagg tgttagttgg attggatacc tattaccacc agggggaatt ggttttaaga
      541 ttagtttatt atatcaaatg acatcattta atttcttaca cttaggaggt atgagttttt
      601 ggacaccaca cattatactg tttttctcag tgatagaaat acctattttt atatttttgg
      661 ctattcgtac ctattgcaaa catcaagtat cgtaacaatt tgatggaaat aatagtgata
      721 taaactagag gatacaataa ttatatatgt agtataaaag ttaaatttag ttaacaaatc
      781 ttaatagcag atataggtaa taaattatac ataaactgta ttattatcta tatctgctat
      841 tttaatatag agaaacaaca gtatattgta aagtaaatgt caaatatagt tttaaaagta
      901 aaattaatgt taaaaagtaa atataatttt acacttgctt aacttaatcc taatatagat
      961 ttaacttaaa aaaagtagaa taaaagtata ggaaatttga tgaagacgaa ttataaaaaa
     1021 ttaaattttg aatacagaac gaaaatcgat aagtatattt taatgggagg gtattatgat
     1081 agtaaaatct agattagtag gtttagaaaa aaatgacaat aatggaaata agacaaaata
     1141 cgtaattgaa aaagtagcaa aaaatatttt tctacttagt gctttagtag cagttgcaag
     1201 tttacttttg ataataggat tcgtatttta taaaggatta agaccattta tatttgaagg
     1261 atattcattt atagactttt taactggtgc agattgggtt ccttctgcca ataaatttgg
     1321 aatatcatct atgatagtag catcaatatt tgcaacactt ggagcacttg ttataggagt
     1381 acctgttggt atattaacct ctgtatttat agcagaagta gcacctaaaa gattagcaaa
     1441 aataatgtca ccagcagttg aacttttagc aggtatacct tcagtattat atggtgtatt
     1501 tggtcttgca gtgatagttc caactataca aagtatattt aatcttccaa aaggtcaaag
     1561 tttgttagca atagtaatag tattagcagt aatgatgtta ccaacaataa tatctgtatc
     1621 agaaacagca ataagagcag taccaaaagc atacaaagaa ggttcattag cacttggtgc
     1681 atcaaaaata gaaacaatat tcaaagttgt attacctgct gcaaaatctg gagtattagc
     1741 cgcagtagtg ttaggagtag gaagagcttt aggtgaaaca atggcagtaa tacttgttgc
     1801 aggcaactca ccagttatgc caactgcaat aacagatagt gtaagaccac ttacaactaa
     1861 tatagcactt gaaatgggat acgcatttgg tacacatcaa gaaatgctat ttgcaactgg
     1921 agtagtattg tttacattta tattaatttt aaacttagtt ttaaataaac tttcaaataa
     1981 ggcggtaaat taagatgaga aagttcaaag agaatctatt gaagtcatta gtatatctat
     2041 cagcactatt tacaatagta tcattagtag ttatagtggg atttatattt ataaagggaa
     2101 ttggcaacat gaattttaat ttcttattta gcaattattc agcatcaggt gatggtggta
     2161 tacttccaat gataattaca acattatata cagtagtagt ttctatagtt gtagcaactc
     2221 ctataggaat attggctgct atatatttac aagaatatgc taaaaaagga aaagcagtta
     2281 cagctataag atttgcaact gaaagtttat cagggatacc atctatagta tatggattat
     2341 ttggaggaat tttctttgta gttacattaa aaatgggata ttccatagtt gcaggttcac
     2401 ttacagttgc aataataata ttaccagtaa taattcgtac aacagaagaa gctttaaaaa
     2461 cagttcctca agcttataga gaatcatcat tggcattagg agctacaaaa tttcaaactt
     2521 tatataaggt agtacttcca agtgcaatac caggaatttt atctggagta atcttatcag
     2581 ttggtcgtat aattggagaa tcagctgcaa tattacttac agcaggtact gtagccaaga
     2641 tgccaggtgg aatatttgat agtgcaagaa cattaactgt acactcatac ttactgacta
     2701 aagagagtgg agatatagca acagcagcca gtataggaat tgttttaata gtaattgtgt
     2761 tagcactaaa catgctagca agatttgttg ctaagaaatt aaataaagct aattattaaa
     2821 tttaagaggt gtgaataatg gaattaatcg ataagataaa aatgagtgta aaagatttgg
     2881 atttatttta tggtgataag caagcattaa agaaaataaa tatggatata aaagagaata
     2941 aggttactgc tttaataggt ccatctggat gcggaaaatc aacttttata agaacgttaa
     3001 atagaatgaa tgacttaatc gaagatgtaa caattaaagg aaacatttct gttgatggag
     3061 aggacatata tacctcagat gatgttataa atcttcgtac taaagtagga atggtatttc
     3121 aaaagccaaa tccatttcct atgagtatat atgataatgt ggcatatggt cctcgtacac
     3181 atggactaag agataagaag caattagata aaattgtaga agaaagctta aaaggtgcag
     3241 ctatttggga tgaggttaaa gacaggctta aatcatctgc actaggactt tctggtggtc
     3301 aacaacagcg tatatgtata gcaagagcaa ttgctatgag accagaagtt attcttatgg
     3361 atgaacctac atctgcttta gacccaattt caacattaaa agtagaggaa cttatagagg
     3421 atttaaaaaa agactacaca atagtcatag ttacacataa tatgcaacaa gctgctcgta
     3481 tatcagatga aacagctttt ttcttaaatg gagaggttat agaatttagc gatacaaaaa
     3541 ctatgtttac cactcctgta gataaaagaa cagaagacta tataacaggt agatttggtt
     3601 aaaatatata actataaagg agttagacaa tggtcaataa tagcttagat ataagtattg
     3661 atactttaaa agaacaaaca gtgcttatga tggaaaaatg tgaagaagca atagaaaagt
     3721 ctgtgacttg tatgatagag aaggatttag agggtgcaag aaaagtagta gaagatgatg
     3781 aagaaataaa taaattaaga gaagaaataa gagataagag tatagagcta atagtattaa
     3841 agcaaccaat ggcaagggac ttaagattta tttattcatt aggtaatata tctatagagc
     3901 tggaaagaat aggagattat gcagcaaata tagctagaga aatgttgaag ataggtggag
     3961 aggcttatat aaaagagcta atagatattc ctaagatggc aaaagaatgt aagtctatga
     4021 tgtctcagac tagagaagct ttaatcttat ttgatgcaag tatagcttat gatgttggaa
     4081 ctaaagatga tattgtagat ggattgtatg aacaagtaca aattgattgt ttaaaaataa
     4141 tgcatgaaaa ctcaaatacc atagaccaag gggtaaaact tctacttata ggaagatacc
     4201 ttgaaagaat tggagaccat ataacaaatg tatgtgagaa gataattttt gcaattgagg
     4261 gtaaaatggt tgagatagga taaaaaactg ctttaaagca gtttttttat tgtctaaatt
     4321 aagaaagaat tgataaaaaa atatgattaa tgggtaaatt taaaaaattg tgatatacta
     4381 ggaacatatt aaatatgagg atgcgtattt tatataaggt gctttttagg aggtggaact
     4441 ttgaaacttc aaaagatggg tatgaggact ataaaaacag gaattagtgt cactttatgt
     4501 atgattcttg gtgactattt agtagagaat atgttttatt ctctggtagc atgtataatt
     4561 tctgttcaag atacagtaaa aggttcttta aagacgggaa tcaatagagt ggaaggtact
     4621 attttaggtg gtataatagg attcatattt gccatcatta aagctggaga cccattttta
     4681 tgtggtctag gagtaatgtg ctcaatatat ttatgcaatc tgtttaaaat aaagtcagta
     4741 gtagttgcct gtgtaacctt tctggctata catctaggtg taggaaatag tgaccctgta
     4801 tattattctt tacatagggt gttagataca tctgtgggtg ttttgtttgg agtacttgtt
     4861 aattattatg ttgctagacc aaattatcta aatagtgtta tagatgagtt taaaaagata
     4921 gaagaacttt caatgttgct tattgaagat aaaattctta gaaaagaaaa tttaaatgta
     4981 gagaaatttg aagaagaagt agaaaaatta gaagaaatat attcaaagct tattgatgag
     5041 ttggattata gtttgagtga aactaatata gaaaaaattg agaatgcctt aaggatatgt
     5101 agagaaatac attttcatat gcagtctata gagttacttg aaaataaact ttatttgaaa
     5161 gagcatacat ataaatacct taagaaaaca tataatgtgg ataagctaga ctggaatata
     5221 gatgaagaaa aaagccctgt attcaactat catctaatga aaataatgat tgaaatagag
     5281 ttgttgagaa agctaaataa atcatattaa taatagtagt taaataataa ataaaaaata
     5341 gtatctttgt aaatattaaa catatataag tttaatttat ggaggtacta ttttttatga
     5401 atactagaaa tgacaattct aaaatactag aaatatataa aatgaagctt tttggcgaac
     5461 ttgtaaaagc agtgtggaat gatgatatag aggatattga aaacttacct aataaaattt
     5521 taaatgaaga tttaaaggat aatttaaata attattttag tggaaaattt gataaagata
     5581 ctatggtaaa tttaattagg gtaactatgg gattggatgt gacagaagat acagataaaa
     5641 atttaaaaga gtcactacat gaagctgtaa atttagatga agtcaaaaga ccaataatat
     5701 ctgtaatatc agatgcttgc aaatattgtg atgaaaataa taaggaagat gagtcatgta
     5761 agataagaag taagcatatt aattgtaatg aggaagatgt atgtagtgct tgtggtgatt
     5821 gtgtatcaag atgtaagttg ggtgcaattt cagataaaat agaatttata ccaatgataa
     5881 aattgttaaa agatataaag agccctgtat atgcaatagt tgcaccagct tttgtaggac
     5941 aatttgggaa agatgtaagc cctggtaaat taagaactgc attaaaaaag ataggatttg
     6001 aagatatgat agaagttgct ttggcagcag atatgcttac tgcaaaagaa gcatatgatt
     6061 attatgagca tatgaaaaag gacgaagaag gatgctttat aactagttgt tgctgtccta
     6121 tttgggttag tttgatacaa aatagttttc ctcagatatt ggaaaatgta tcaccatccg
     6181 tttctcctat gattgcttgt gggagagctg taaaaatttt aaatccagat gctaaagttg
     6241 tattcatagg tccttgtatg gcaaaaaaga aagaggcaat gttagaagat ataaaagatg
     6301 cagttgattt tgtgcttaca tttaaagagt tgcaagaaat atttgatgca ttagagttaa
     6361 atccagcaat aatggatgag agtaatagag tagaggcgtc cctttcaggt agagtttatg
     6421 ctagaacagg aggtgtaagt aaagcagtta agttatcagt tagaagaatt gacaaaaata
     6481 taaaatttga agaaaaggca tttgaaggaa caaaagagtg catggacggt ctaaagaaag
     6541 ttctaaataa agaaatagaa gctacattta tagaaggaat ggggtgtgta ggaggctgtg
     6601 taggaggtcc gaagcgtatt ttaagtgtgg aagaaggtac taagaatgtt gatgaatata
     6661 gtgaaagtac gaatatggaa actccatttg ataatttaaa tgtattacaa tttttaactt
     6721 ctttgggaat caaaagagta gaatctttag gagaaaaaga agaagaaaag gttaaaaaaa
     6781 tatttagtag agatataaaa agtaataaga aagagaataa atataaaaaa taacttttta
     6841 agtcttaaac ttatgctttt ataaaaggga gatatggggg atgaaaatta aagataataa
     6901 ggctgttttt catgtattta taaggatatt tcttatagct gttttaattt taactggaac
     6961 attattggag gaaaaaatta ataatgttcc agttgaaaat ataagttcaa aaggtgaaaa
     7021 tacactggaa ctgctttctc aaagtaatac tttaggagaa atcatataca aagcatctca
     7081 agaagaaaca aaaggtccag gggctacttc tgaaaaagta gcatacataa caatagatga
     7141 tggtccatca aaatatacaa atgcactact tgatatttta aaagaaaatg atgtcaaagc
     7201 gacatttttc atgcttaatc agaatatgaa aaaccacaag gaagaagtaa ggagagtctt
     7261 ggaagaagaa aactcaatag gttttcatag tgtaagtcat gatatacata agttatataa
     7321 aagcccagaa gtaacagttg gggaatttaa tacatgcaat aatactctac aagaaataac
     7381 tggtcagagt tcaaaattaa taagattacc atacggaagt aaaccatata tgccagaagg
     7441 ttcatataat aaacttatgg caaatgatta tttaatttgg gactggaatt tggatacaca
     7501 ggattggaag tcttctacaa gtcagatagt aagtaatata ttatattatg gaagaaaaag
     7561 agatgaatta gtagtattaa ttcatgagaa agagcaaagt ttaaatgctt taaataatat
     7621 tataagaatc ttaaaagaga gaggatatac aatattgcca attacagaaa atataacacc
     7681 taaaaatttc tggcaagaaa acttataata aatttaaata tagtaaaaga taaagtaaat
     7741 tatttataaa ataaaacata taatataaaa aatacgaata agaggtatac tgtaaggata
     7801 atataaaatt aatgagaaag cattaaaatt aaaataatct tgcaattatt gacattataa
     7861 gacagaataa ctgtaaaaac atattttaaa atataacaac tatataaaag agaatagaca
     7921 taattaagaa aattcagaaa aaataaaata aaaataattg acaaagagaa taatatttgt
     7981 atataataat atgtaatatt aaagactgag aagaagagga gtaagtaaat actactaaaa
     8041 gagaggaaaa ttcactggct gtaagatttt ctaagtataa gatttactga atgtagcttt
     8101 ggagtttcta aactgaacta taagtaagtt tagacggtta gaaccgttaa attctttgag
     8161 agtcaaagtt gataaaataa cattaaccaa taatttaatt atatgttaat gtattttaag
     8221 ctttggaatt aaggtggtaa cgcgagcttt tcgtcctttt taaagaggat gaagagctct
     8281 tttttatttc taaagatatt taaaaaattt atttataaaa tacaggaggc aggacaatgg
     8341 aaaatacgaa tttatcaaaa acttataacc caaaagattt tgaggctagg ctatataaaa
     8401 aatggatgga tgaagggtat tttaaatcta aaccaaatcc agataaaaaa ccatttacaa
     8461 taatgatgcc accaccaaat ataacagggc aattgcatat gggacacgct cttgaccata
     8521 cacttcaaga tatattaatc agatggaaac gtatggatgg atatgaagct ttttggcttc
     8581 caggaactga ccatgcttct atagcaacag aagttaaagt tgttgaaaga ataaaaaaac
     8641 aagaaggcaa gacaaagtat gaaataggta gagaagaatt tttaaagaga gcctgggaat
     8701 ggaaagatga atttggagga aaaatatcta atcagcttaa acaactaggt gattcttgtg
     8761 attgggataa agaaagattt actatggatg aagggtgtaa tgaagcagtt atagaatttt
     8821 ttgtaagcct atatgaaaaa ggtcacatat acagaggaaa tagaataata aactggtgcc
     8881 cagattgcaa aactacttta tcagatgcag aagttgaaca tgaagaacac gacggaaact
     8941 tttatcatat aaagtaccca ctaaaagata gtgaagactt tttagaaata gctacaacta
     9001 gacctgagac aatgatagga gatactggta tagcagtaaa tccagaagat gatagatata
     9061 aacatttaat aggaaaaaca gcaatacttc cattagtagg aagagaactt ccaatagttg
     9121 cagatagtta tgttgattta gagtttggaa ctggtgcagt taagatgact ccagcacatg
     9181 accctaatga ttttgaagta ggattaagac ataatcttga gcagttaaat actatgaatg
     9241 aagatggaac tatgaatgaa gtttgtggaa aatatgaagg tatggataga tttgagtgta
     9301 gaaaagctat cgtagcagac ttaaaagaac aaggttacct aattaagata aaagagcata
     9361 atcataatgt aggaacttgt tatagatgcc atacagtagt tgagcctaga ttatcagagc
     9421 aatggtttgt taagatggaa gaacttgcaa aaccagctat agatattctt aaaaaaggag
     9481 aattagaatt tgttccagat aaatttgata aaacttattt acaatggcta gaaaatataa
     9541 gagattggtg tatatcaaga caattatggt ggggtcatca aataccagct tattattgtc
     9601 aagagtgtgg agaaatagta gttgctagaa aaatgccaga taagtgtcct aaatgtggaa
     9661 gcactcattt taaacaagat gaagatgcct tagatacttg gttcagttct gcattatggc
     9721 ctttctctac tctaggatgg ccaaataaga ctgaagcttt agattattat tatccaacaa
     9781 gtgtacttgt tacaggatac gatataatat tcttctgggt agttagaatg gcatttgcag
     9841 gtatgttctg tatgaatgaa aagccatttg accatgtttt ggttcatgga ttagtaagag
     9901 attctcaagg tagaaaaatg agtaaatctt taggaaatgg tatcgaccca ttggagataa
     9961 tcgagcaata tggagctgat gccctaagat ttacattaac aactggaaat tccccaggaa
    10021 atgatatgag attctatatg gaaagagtag aatttgctag aaactttgca aataaattgt
    10081 ggaatgcatc aagatttgta tttatgaata ttgatgaaga tataattaaa aacatgacta
    10141 gagaaagtgt taaagaggac ttaactctag ctgataaatg gataatatca agagcaaata
    10201 atattgtaaa agaagcaact aataacatgg ataagtttga tttaggaata gcacttcaaa
    10261 agatatacga ttttacttgg tcagaatact gtgactggta tattgaaatg gttaaaccta
    10321 gattatatgg ggaagatgca aatgctaaat ctgcagcatt atatacatta acttatgttc
    10381 ttgaaaaaat attaaaatta ttacatccat acatgccatt tataacagag gaaatatata
    10441 ctcatcttcc tactgttgaa ggatgtataa tagtatctga atggccaaaa tataatgaag
    10501 aagataacat ggctgaagaa gaagatatga tgaacttatt gatggaaggt ataagaagta
    10561 taagaaatgt gagagcagaa atgaatgtac caccttctaa gaaagctaaa ttgataataa
    10621 tcccaagtga agaaaaaata gaagctattg agcttggtaa agactatttt ataactttag
    10681 catctgcttc aaatgtagag atagcaaaag ataaatctaa tgttccagaa gatgcagttg
    10741 gagtagttat tgatggtgtt gaaatattta tacctcttaa tgaattagtt gattttgaaa
    10801 aagaaattga gagattatct aaagaaaaga aaaagttaga aggcgaaata aagagagtta
    10861 atggaaagct tgcaaatcaa ggtttcttag caaaagctcc tgaaagttta attgaagaag
    10921 aaaaagcaaa aaaagaaaaa tttgaagaga tgattaaatc tgttgaggaa agattaacaa
    10981 atttagaatc taaaataaaa taaatataag aattagtaaa aacatacaaa aagtatatat
    11041 tctatacttt ttgtatgttt ttctttatat atatacaaat atgatgttat aattctttac
    11101 agatgtgtta tatttttatt ttatgttgtg aatatatgaa ttttatgtct taaaat
//
LOCUS       DAELNY010000121        11038 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.121, whole genome shotgun sequence.
ACCESSION   DAELNY010000121 DAELNY010000000
VERSION     DAELNY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.121"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..614)
                     /locus_tag="KPE55_002624"
     CDS             complement(<1..614)
                     /locus_tag="KPE55_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0493678.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSG"
     gene            complement(686..1039)
                     /locus_tag="KPE55_002625"
     CDS             complement(686..1039)
                     /locus_tag="KPE55_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG0493679.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1117..1320)
                     /locus_tag="KPE55_002626"
                     /pseudo
     CDS             complement(1117..1320)
                     /locus_tag="KPE55_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            complement(1710..1940)
                     /locus_tag="KPE55_002627"
     CDS             complement(1710..1940)
                     /locus_tag="KPE55_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0493680.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1942..2349)
                     /locus_tag="KPE55_002628"
     CDS             complement(1942..2349)
                     /locus_tag="KPE55_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0493681.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2710..3148)
                     /locus_tag="KPE55_002629"
                     /pseudo
     CDS             complement(2710..3148)
                     /locus_tag="KPE55_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3162..4088)
                     /locus_tag="KPE55_002630"
     CDS             complement(3162..4088)
                     /locus_tag="KPE55_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493682.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4213..4995)
                     /locus_tag="KPE55_002631"
     CDS             complement(4213..4995)
                     /locus_tag="KPE55_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG0493683.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5151..5771)
                     /locus_tag="KPE55_002632"
     CDS             complement(5151..5771)
                     /locus_tag="KPE55_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0493684.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5898..6566)
                     /locus_tag="KPE55_002633"
                     /pseudo
     CDS             complement(5898..6566)
                     /locus_tag="KPE55_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6658..7473
                     /locus_tag="KPE55_002634"
                     /pseudo
     CDS             6658..7473
                     /locus_tag="KPE55_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8144..8911
                     /locus_tag="KPE55_002635"
     CDS             8144..8911
                     /locus_tag="KPE55_002635"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0493685.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9244..9921)
                     /locus_tag="KPE55_002636"
     CDS             complement(9244..9921)
                     /locus_tag="KPE55_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493686.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10071..10388)
                     /locus_tag="KPE55_002637"
     CDS             complement(10071..10388)
                     /locus_tag="KPE55_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493687.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10410..10748)
                     /locus_tag="KPE55_002638"
     CDS             complement(10410..10748)
                     /locus_tag="KPE55_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493688.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3672 a         1729 c         1564 g         4073 t
ORIGIN      
        1 tttcctgatt gaatatgctt aatgtttgat tgaatttttt tatatggcca atcccaccat
       61 ttaatttcta ataattttga aattgtatca ttattaaatc tcttttttat aatttttgca
      121 ggtactcctc caacaataga atacggtgga acgtcattag taactactgc tcttgttcca
      181 ataattgcac catcaccaat atttacacct gacataatta tagaatcata acctatccac
      241 acatcatttc caattataat gttaccctta ttgtcccaag cgtccgttat atggttgata
      301 tcgagtttcc attcttcata aaagatggga aaagtatatg tggattttga tttcattgag
      361 tgatttccac tagtcattag aaattttgca ttacatgcta ttgagcaaaa cttaccaatt
      421 attaatttat cattgtttat agggtaatga tacaatacat tgtttttctc aaaatctctt
      481 gggtcattat aaaagtcatt atatattgtg taatctccaa cttctatatt ttcccttgtt
      541 attacatttt taagatatac agtttgataa tcattacttc ttggatatac cttatttgaa
      601 cttggaatag tcatatttaa atctctcctt taatatatat tatgtttttg actattccac
      661 tactacaata catctcttca cagaatcact ccttgttaag aatcttcata agataatggc
      721 gtcctttttt agtgttaagt cggatattct ttcttagtac attcttttta gcgtataact
      781 gacaaactgt cattttctta ccaatagtgt caatgctatc atcaaggtca ttttgtattg
      841 ctttttcctt atcccttaat gatatatcat cacgttttcc ctttgggggt ttatcagtag
      901 gtacaagttt ccaagagtaa acaaattgtg ctttaccaaa tgaatataca tacttatatg
      961 cgtatcttcc atcttttctc tggctctctc cagttcttaa aattctatct ttattatctc
     1021 gtcttttttc agttgacatg ataagaagct cctctcaata accgaattga gcttgatacg
     1081 tcatatgaat attataacat ataaggctca atttcattag ataacgtcaa gtgtatcaac
     1141 gattttctca aattgttttc tctttatttg aatgcgattg ctattcataa tgacccagcc
     1201 agaatcaagg ttttcatcag caagtttacg taattttttc tctccaatac gaaagtattt
     1261 tgacgcttat tcaatcgtca aggtatattt ttcccaaata ggtacatcag tattgttcat
     1321 agaatgtatc actcctttct atgttcgtag ttcagaagta gatagatggc tttggtaagc
     1381 tccattggta tttcaaccaa tcccattctt atgggcagaa ccgtgtcata caggtgtatc
     1441 attatactgt ggtacaggtt acggcaaaac aaccgttcca aaggttattc acaaatcact
     1501 atatagtagc tctcttgatt aaggtcatgg cgtatagtat ttgttggcta tctgtatcac
     1561 aaacgtatct atctacttta ttcagttgtc aaagaacagt cggcattgat ttgcctataa
     1621 aaaatcacaa tagcttatgg gcttttatag acaaataatt agtgattctc catgtaccaa
     1681 atagcttgat acatggagag agggcgagat taagacaaat taaatctcaa aatagcagtg
     1741 attagttttg ttcctagcat tcgctttgtt tcttcttcta catgaatata gggatttccg
     1801 tattcatcaa ataaagtttt ctgtgaaaga cgaatgatat agtgttcaaa atgtttaagt
     1861 attacgttta ttgcatttac atcgccatgt acagcaagta aaataattgt aaaaggtaat
     1921 acttctttag taggtttcat ttcaatgttc ctgcatgagt tctttcaact cttttagtgc
     1981 atttgttttg tgataatgta tggtattttc tgctaaatcc atcaatgttg ctatgtcagc
     2041 atttgtcatg ttgagaaaga atgaaagtag aattatatct tgttttcttt ttgtaagttt
     2101 tccgattgct tgtgccatta atacatcttc aattcgtatg ttgtagtcgt aagaaaccat
     2161 acagatgtag ttagaatcat aatcatctaa aatactcaat ttacttaatt ctgcaagtga
     2221 caaagattcg agagatacca aataatcatt tctgcgttta ttttcgtcat aaatatctct
     2281 agcatagtta cgcaaaactg ttttacagaa actgtcgaat tgatgttcaa ttgtcttttc
     2341 atcaaccata agatttcacc ctctttccat cgcaaggagt taaataacct tgcttactcc
     2401 tatcacggtg ggagggcgtt aaatcttcga taaatttcaa aaaaaaagag aaaaaaatac
     2461 acactctgcc tatagggcaa aaatgtgtat tctttaaaac gtatttaatt tatattggtt
     2521 gtaagccacc accatattga tgtatggaaa tatgcattgg ctagcttttc ataaaaatac
     2581 aaagatgcat aagaaattaa tagaggggaa aaacttattc tgttattaaa aagtaggtta
     2641 attctaataa atgctattgc ggtcgaatta aactatatac atggcaatca atcatgtttc
     2701 tattctttat tatgaattgt gacagtattc ttcaatctta aagccacatt ttacttgact
     2761 ctctattgta ggaatataca ttagcatgaa ttttgttaag gttgtatttt tcaaaggcaa
     2821 ttttgcatat ttcttttact gcatttgttg ctatcccttt gccccaatag ttcctagcaa
     2881 gtcagtaacc taattcagca tttttattaa tagaggtatc ttcttcaaat gttaggctga
     2941 tacttccaat agccctttct tgataaaaaa tagcatagct aagttgttta acatctgaag
     3001 ttgtcaaaca aaaactaata aaatctctag cgttttgcta cctataaggg tgggggaagt
     3061 tgtcacgaag attgtttgca atttctttat cattcgcaaa ctcaactaaa tcggtaatat
     3121 aagcataatt ccactctata agttccattt ttcttctcct tttatgaaac agctacaaga
     3181 aatctttcta agccaatacc acttatcaaa cagcgttctt ttttatttcc aagcatttga
     3241 taagtccagt caacaaatcc accgtcaact atttcaacgg tttcatttcc ttgtttgacc
     3301 gataatttga aattgatacc tttataataa ttattatcaa catcatcatt atgaattgat
     3361 aactctatat taggaaaaat aagtttgata gtttcaacca ttctgtcgaa aaatccgtca
     3421 ttatctttgt atccgtttct tttgcgtaga ataatagaga aacgaacatt ttctatttca
     3481 cttaatatat ctttataata gtttaagtgc ttttctaaca acattttttc acaagtgtaa
     3541 gaccctgtat ctataccaga tgaaaccata caatataagc caaaatgtga atataaacct
     3601 ttacctacaa aattttgtgc tcttattacc ctagcagtag tagccatgtg gataggtatg
     3661 gtattatttt cttcttttct ttttaattta tcagcaataa ttattgccaa catatttgag
     3721 gggtctgcaa gtgtttctgt ccctcgtaga gcacttacta cattatattg atctacggag
     3781 ccaaaaacgc tacagctacc taatggagca gagggagaaa gcataatcgt cttgatatcc
     3841 atattgaatg cttttctcaa taattttgac tccaaagcat gaatttcttc tggatctata
     3901 ccgctaggga tagtaaatct atttagttgg tatgacttta gaatatcagt tgcagttaaa
     3961 gtatttgctt gtctatcaaa cacttctaat agaagtgagt tcaaatctga ttttgataga
     4021 gctgataact tatcaactat attcttatcc ccaattttat ttgtaattct acttaaaata
     4081 tcactcatag ttatcctcca tactgttact ttcaaaataa ttatagaatt gttacaacaa
     4141 ctaatctagg tcatttgacc aaaaatgaaa aaacactgaa tatttactat tcaatatttt
     4201 tttactttgg gtttatttga ttaaatcttg aaaatactgt aatgcaaccc tttcagcttc
     4261 aacctttcgg acaataggac tttgtgataa ttcctctctt aattcaagat acaggttgat
     4321 agtagcctgc aatgcattaa cacagctttt atattcatgg ttagcaaccg tcttttctaa
     4381 tgcttcaata tattggggtg cgtccaattc aatttttcta actccacgag gtaatttacc
     4441 taagagaagc tgtattaacg aggcaagtac tgattgtctt aaaaaagata aaaaatcaat
     4501 tgtttcaaat agttctctac gtccaatctt agtgcaagtg taatgaaccc aaatccaaaa
     4561 tctatcttca atccactgta agtctggagt tgggaagtat gcttcttttt tagaataaat
     4621 ttctgttatg cagttgtttt cttcatagag aatagctgaa tcttcaaccc tatctagtaa
     4681 accatctaag gttgtaaact taaaatcaac atgaagaaca ggggaatcat agagggaaat
     4741 tatcaatcga ggttctccaa catgttcgcc agtaaaagca gaaactaatg ttccaaactg
     4801 ttccacaata agtaatctct cttcgataac ttcttcatag gatttatcat ctgctacaat
     4861 cagaaaatcc aaatcagaat attcatctaa cgattttgtg atataagaac cgctaattgc
     4921 aagtcctatt atcctactat ctctttttga aaattctaat acgttttgta tcatttcctg
     4981 ttgtgtttta gtcataatat aacacctctc actgtctttt attagctatc aatccaacat
     5041 aattatgcat aggagtatta tataatgaag aaataaaaaa aaataggtca cttgacccga
     5101 aataaaaaag tattgaataa ttattatcca atactttttt gaaattatac ttatgttgta
     5161 ggaagattct ttaatacctc tcgctgatta attacactga atcttttgtt ttcataagca
     5221 attagatgtt cttgtacaca ttcatttata gcacgattaa tgcttctaat cggagcgttt
     5281 acttcgctag caagctgttt tttggtaagc agatttaatt cacttctata atagtgtaag
     5341 gatatacacc tcagaagtcg ttctttaaca cttaaagatg ataattcttc aattaattca
     5401 gcatttacaa ctaatttact agatatttct cgaatcagaa attttgtagc ttcaaaatct
     5461 ccccttaacc agtttaaaaa atcatctcta tgaagtttat atacattaca atctgtgaaa
     5521 gcgacaattt caacgggttt tttcccatca ttaaattgtt ccatttctcc aaaaaaacta
     5581 ccacttctat aaagatgaat agttgagatg tttcctttca catttaagat gaatgcttct
     5641 gcaataccgt caatcataag aattacatat tcattttctt gctcagcaaa aagaatagtc
     5701 tctttctttt tatacgtttt tttaacaagc ttctttttaa tatcttcatt cataaaatca
     5761 attaaatgca tattcatctc tccttaccaa atctttgatt aaattatatc atctttcaat
     5821 tagttaggta agatagtaaa aatatattta catagaaact attccattat tattttccag
     5881 ttgtcatttt tctgcaaaaa cttatcactg tcacgactgg agataccagt aaggaatgtt
     5941 aggatatcaa tcgctaaact ttcagaatcc tttttacggc tcaaaatgac gtaatggtca
     6001 agcaatcctt tgttttcatc atcactggtt aaattgacaa tgtttatctc atgggctatt
     6061 tctggtagcg tttctttcca tttgccgcgt tttgctttag ggtcaactat gaccgcttgt
     6121 ccgccgaata gtaccgcata gtaaacaagc atattgttac tgaataattt accaccaccc
     6181 aatgaaccga taaacgcaga cgctaacgca ttggtaatag aaccctttac cccttgacta
     6241 gcaagggcag gttttagata gacattcttt ccagtgtcca gattgtagcc aagatagata
     6301 tcctcatgtt cgccaagggt ttgtgtcgct ccaaaaccaa gtccagccaa aaaatccgaa
     6361 gtgacatatt gaatataatc attgatgtaa cgtttgctga caggaataaa ttcgccatgc
     6421 aatcccaaca tatccccaaa gggacgcacg agcttgattg ataagtcgtc gtaaaagtct
     6481 ttgacttcat cacaacgcct ttttagctcg tcaagatcct ttgtagatac acggacaaca
     6541 tagctaagtt tatacataga ttctttacag ttaaaagtct ctttttaaaa aaatgtataa
     6601 attataaatc aaaatataga tactatcaat gacccactag gtgcaaaaag cattgatatg
     6661 gttaaattat cagacgctaa aagctgggct atccgaatga gtgaagatgg gtgtgcttat
     6721 aaatccattg acaattataa gcgctcactg aaagcgtcgt ttttcatggc aattcaagac
     6781 gactgtatta gaaagaatcc atttaatttt tagttaagtg atgttttgga agatacaaca
     6841 gaaccaaaaa tcgctctaac accagaacag gaaatgggct tattatcctt tatggggagc
     6901 gataagactt attataaata ttatgatgat gttgttatcc tcttagaaac aggactacgc
     6961 atttctgaat tatgcggttt gactttagaa ttagatatga aaaataaagc cattatggta
     7021 gaccatcaat tgttgagtga tactaagatt ggctattatg tagaagttcc aaagaccaca
     7081 cagggtaagc gagaaattcc aatgactgaa agagcttatc aatcatttga acgtatcgtt
     7141 gaacggcgta agaacgctaa agcagaacca atcattgttg atggttacca gaacttccta
     7201 tttcttaaac aagatggatt accaaaacaa gcaactcatt atcaaagcat gttaagagcg
     7261 ttagtcaaga agtataacaa gacccatgag gacaagctac caaagattac gccacatacc
     7321 tttaggcata cactctgtac aaatatggct aacaaaggaa tgagtactaa tatattgcaa
     7381 tatattatgg ggcataaaaa cattactatg acgcttggct attatgcaca tggttcattt
     7441 agttctgcga gagctgaaat ggaacggtta taatcctagt agtatgacta gagtttacta
     7501 cttatttact acttttgatg gcattcttat acggttaaat gccaacttat gcaaagtatc
     7561 ttccaaaaag aaaatgtcga tactccttgt agatacagga tatatcgaca tttaccaact
     7621 tatgaaaaga taatcaaatt taatataagt ttttcaaata aaaagatact aaattctttt
     7681 tagagaatct atataacatt acttaagctt ggttggggta taaacaattg agtagctact
     7741 tttgtatggt gttcaacttt agtcaaaact cttaaataat gcaaatgatt tagattcatt
     7801 aatattcaat ctcttcataa gtagtgaaat tcgagttatt tctgcataat tatactatga
     7861 tttatagatt aattctatta gcatccagaa taatattgtt tatatgagta aaatttaaag
     7921 aatattgtga aaattataga atgttaaaat aaatttaggt aaaaaaaagt gtttgtatat
     7981 tatagagtac cttttctaaa atatgtggga ttgttttaag aattagaata aaaattattt
     8041 ttaaattagt atttttataa tgtatagtat ttttaatgta taattaaatt gtgcagaaaa
     8101 tagaatttaa agtcctatag aatttctgat tatggaggag aatatgaaaa gaaaagttca
     8161 agtaaaaaat attactattg gagaaggtag acctaaaatt tgtgtaccaa ttattgggaa
     8221 aaataaaaaa gatattataa aagaggctaa agagttaaaa gatgcatgtt tggatattat
     8281 agagtggcga gttgattttt ttgagaatgt agaaaatatt aaagaagtta aggaagttct
     8341 ctatgaatta agaagttata tacatgatat ccctttatta ttcacattta gaagtgtagt
     8401 agaaggggga gaaaaattaa tatcaagaga ttattatact actttgaata aagaaatttc
     8461 taatacaggt ttagtagatt taatagatgt agagttattt atgggagatg aggtcataga
     8521 tgaagtagtt aattttgctc ataaaaaaga agtgaaagta attatatcta atcatgattt
     8581 taataaaact ccaaaaaaag aagagattgt atctcgttta tgtagaatgc aggaattggg
     8641 cgcagattta cccaaaatag ctgttatgcc gcaaaatgaa aaagatgtgt tagtgctact
     8701 tgaagctaca aatgaaatgt ttaaaattta tgcagataga ccaataataa caatgtcaat
     8761 gtcaggaatg ggtgttataa gtagattgtg tggtgaaata tttggttctg cattgacttt
     8821 tggagctgca aaaagtgttt cagcaccagg acaaatatca tttaaagagt taaattcagt
     8881 acttaatcta ttacataaga gtattaatta aattaattat atagaaatat tagaaggtat
     8941 tttatttttg aagatataaa ttaaatataa aaaacttttc agatagtaaa atttaaattg
     9001 ggcaattata tctgaaaagc aactatttag tgtgtatttt gaaagtgata tagaaaatct
     9061 tataaatcaa ctatggcata tttaaaatat gccattaaaa cctactagac attagattat
     9121 agatggtata ctagcatttc tattatttat agatagtaag ttttcataat aactgtttta
     9181 tctaaagtag aagatatatt tgtattatgt atatttgtca ttattttttt gtaattatgt
     9241 atattaatgg ttatatatgt aatataatat tatcactcta ttttttgtat attgtacatt
     9301 acaacattta tatctatcat catcaattac agtagaatgt atgggatact tattttcact
     9361 taaccaatat ttttgcatat tctttgtcca agactttgga aacttcaata cttttttgtt
     9421 tattataaca tcctcatctt caaaaatatt aacttctctt tctattttta tttttctgtt
     9481 gtatatctta ttgatagatt ctttgtcgag ttttatgtcc ctatacatat cagggcaata
     9541 gcaacaaaca taatttcttt tttttcgacg tttaaatttc acaactttct ctatgctaat
     9601 ttctaatttt tttgtaccat cttttataaa tttaatatct tctatgttct cgcttgaaaa
     9661 aatttctgtc aatcttaaag tatctacagt ttctctaatt atttgctttt tataagaaga
     9721 gaatccatat ccaaaagaaa ggtttaattt attagggatg tagccatttt tatatttaaa
     9781 catcttaatt ttttccaatt cagcattatc tttaaatttc aacttttcat acctaaaagt
     9841 tactttgcta aaaagagtat attctacatc aatatattct tttgcaatct taaatttatc
     9901 gctaccttca atcacaatca tagcctatct ccaatcgttt tatattaaaa taaggccata
     9961 gaacatggtc taatagtact cattagcact ataagacaat gttaacacaa aaagacaaaa
    10021 aaaggttatt tatttcaaat aacctttttt tttatataaa atataaagaa ttatatttta
    10081 ttctttttta tgatgtctat aatctgtgat atcagattta gttctcttga atttaagtct
    10141 tttaactctt catttatatc attttctaaa tatgtttttc cagtggaagt aatatctttt
    10201 ataagaacat ctgcagaaat atttaatata tttacaaatt ttatgaatgt tttaagactt
    10261 ggattctttt taccactttc gatttgagtt atataccaag gcgatactcc aaccatattt
    10321 gcaaattctt ttgaagttaa atttgctttt ttccgttctt ctttaagtct tttacctata
    10381 taattcatta ttgttactat ttccctcact tatatttctt tgtatttaat gatgcacttt
    10441 attaactcta gcattgcaat ttgttctttt tcagttaatt tattaatcat ttcataacat
    10501 tctaaaggta cagagttatc aacgccttct gttgtaataa tatttgcaaa taacaattca
    10561 agtggtatgt ctaaagcagt agcaattttc attataatat tgatagatgt atttgtctca
    10621 gctctttcaa tctgaccaat atatgttgga tgtaaacctg ccttttctgc tagaaactct
    10681 tgactatatc ccaattttgt tctatagact tttattctat ctcctattat ttttcctata
    10741 tttgccaatt attatccctc cactttagat tatattcata gtttaagttg ttttatatag
    10801 actatttata cttttttagt ctaaatatag actatatatg tttttatact gaaataagtg
    10861 ctatttattt ttgtatagtc tgtatctaca ataaacaaat atggataaat catattttta
    10921 attagagaaa tattttcata atttcatagc catactacaa taataaaacg aattaataat
    10981 atgttaatta tagcattttt tattgtaatt acatattaga tatgtagaat atattttg
//
LOCUS       DAELNY010000122        10938 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.122, whole genome shotgun sequence.
ACCESSION   DAELNY010000122 DAELNY010000000
VERSION     DAELNY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.122"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            300..1742
                     /locus_tag="KPE55_002639"
     CDS             300..1742
                     /locus_tag="KPE55_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0493689.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1784..2137
                     /locus_tag="KPE55_002640"
     CDS             1784..2137
                     /locus_tag="KPE55_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493690.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2328..3149
                     /locus_tag="KPE55_002641"
     CDS             2328..3149
                     /locus_tag="KPE55_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0493691.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            3407..5290
                     /locus_tag="KPE55_002642"
     CDS             3407..5290
                     /locus_tag="KPE55_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG0493692.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IRIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMKWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5515..6195
                     /gene="pgmB"
                     /locus_tag="KPE55_002643"
     CDS             5515..6195
                     /gene="pgmB"
                     /locus_tag="KPE55_002643"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0493693.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            6192..8477
                     /locus_tag="KPE55_002644"
     CDS             6192..8477
                     /locus_tag="KPE55_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0493694.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            8702..9595
                     /locus_tag="KPE55_002645"
     CDS             8702..9595
                     /locus_tag="KPE55_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0493695.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            10032..10523
                     /locus_tag="KPE55_002646"
     CDS             10032..10523
                     /locus_tag="KPE55_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493696.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEYEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            10551..>10938
                     /locus_tag="KPE55_002647"
     CDS             10551..>10938
                     /locus_tag="KPE55_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493697.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCN"
BASE COUNT         4482 a         1011 c         1885 g         3560 t
ORIGIN      
        1 tatttattgt atacaataaa atttcatttt gtaaaacagt gaattaaata tgtaatatag
       61 atatcaattt ttattacaaa ttgttactaa atataggtac attagaacaa aaatctagta
      121 aattgtaact aaaatgtaaa tttttccttt aatctataca atattatgga aactttttgt
      181 aatttatgat ataataacaa atatataatt attaaattat aataaatgac tatagcgtag
      241 atgtaatact atgttatagt catttttgaa ttaaacttga tatatcaagg gggaagaata
      301 tgagaacaat tagaagagtt ttagcattag gtttaacatt agctatattt ctaataaatg
      361 tacctaatgt agatgcatta acatcagata caattaaagg taacaatata tatgagacgg
      421 ctggattaat agcagataaa aagagttatg atacagcaat tatggtaaat atggataatt
      481 ctatagcaga tggtctttca gcaagtggtc ttgcaggtgc tgttgatgct ccaattttgc
      541 ttgcacaaaa aaataagata ccaaatgaaa caaaacaaag attaaaaaat gttaaaaaga
      601 tatacataat aggtaaagaa ttatcaataa gtaagtcagt agaaactgaa ctaaaaaaca
      661 caggggcaca ggtaactaga ttaggtggag atgacagaat aaagactagt tatagtgttg
      721 ctaaagaggt aaatggtatc aaaaaagttg atgaagtgat attaactaat gcatataaag
      781 gagaagcaga tacaataagt gctgcacctg tatcagtaag agatatagct cctatagtac
      841 ttacagatgg aaagagtgtg cctttttcaa caagcaatgt aaaaacatat gcagtaggtg
      901 ggagtatatc aatgagcact agtttagtta ataaaactaa tgctaaaaga cttggtggtt
      961 cagatagata tgatacaaat aagaaagtta taaaagagtt ttatccagat gcatcagaat
     1021 tttatttgag tgatggatat gatttagtaa atgcacttac aggttctaca attgctaagg
     1081 aaaatccaat tgtattggtg tcagaaagta gtgataagtc tatattagca ggagcagata
     1141 agattactag attaggttca ataagtgaca gtgtgtataa taagtgtgtt tctgctgcac
     1201 aaaataatgg cgattcgtct acaaaaggtg agtcacctat gaaaaatgag acaagcatat
     1261 taggtcagcc aacagctagt ttagaagcgt gcttaaaatg ggcaaaatct aaaaaagcta
     1321 atgatttatt tatagagtta ataccaatat tatatgatac tgctgttcag gaaggtgtta
     1381 atcctgtttt ggcagtagct caatctgcaa aagaaactgg tttttgtaat tttggtggag
     1441 tattagatgc atcatttaag aatccttgtg gacttaaaac ttctgtaggt ggttctgata
     1501 ctgataagaa tgcccattca agatttgata cttgggaaga aggaatatta gctcaaattc
     1561 aacatttatg cttatatgca ggacaagatg gttacccact ttcaaatcca gtagacccta
     1621 gacatgaaaa atctttattt ggtaaggcta aaacagttga aagtttatct aataattggg
     1681 ctggaggtca atatggacaa gatttagtaa gaatgatggg agaaattgag gcaacaaaat
     1741 aattgaagat tatcaaatat ccaataaata aggattgatt attatgaaaa agagtaatat
     1801 ttttagtatc atatttccta cagtcataat ggtgctaatg tttttaactt ttgctacaga
     1861 tatatttaat attccagata ttcaatctaa aagtattttt gttgtgggaa tggttttgat
     1921 ttttccaatc tcttttttga tacaagggat aatttgtgta ttaaataaga ctaattggat
     1981 tttatctctt attgtgtctt tagtaactta tatagtatta atgtatatat ttttaaatga
     2041 ttctgcatat atatatgttt tactttatac agctttttat atgattggac atataacagc
     2101 taaactctat tacaaaatta agacttttaa aaattaaatt gaagtatata aaagtaacat
     2161 taagagaagt atccttaatg attttctaaa atcagaaata ggtgcttctc ttttttgtaa
     2221 aaataaaagt atgtaaataa aatttaacca ttataatttt cttttaacaa atgtatacaa
     2281 taaacttgtt gtataattaa gatagataaa ataaagggag ggataatatg aatttaatag
     2341 ataatcatat tcatactaat ttttcaagtg atggaaagga ttccatggaa gatacaataa
     2401 aaaaagctat aagtattggt gttagatatt tgacttttac agaccattta gaacatgatg
     2461 aagatagagg attttgcata aattataatg actatgttcc agtatttaat aagtttaaag
     2521 aaaaatataa aaaggacata gagttattac taggtgttga agtgggttac agaaaacatc
     2581 taaaaaatga aatagaagaa ataataaatt ctaatccttt tgattttgta ttgtgctcaa
     2641 ctcatacaat tgacaatgta cctgttccat caaaggagta ttttaaaggt cttagcaaag
     2701 aagatgcgta ttataaatat tttaatagca tacttgaaac taatcgtgaa tttggagatt
     2761 acaatatata tggtcatttg gattatatat ctaggtatgg aatatattca gataatagag
     2821 ttatatacaa tgactttaaa gatgttattg atgaggtctt aaagtcaata atcaataatg
     2881 ggagtggtat agagctaaat acttctggtt atagatatgg actaaatgct atacatccaa
     2941 atgaagatat tctaaaaaga tataaagaac ttggaggatt tatagttact gtaggttctg
     3001 attctcatag ggtagaagat atatgcaaag attttgatgt ggcctatgat atgcttaagt
     3061 atcttgactt taaatatgta tctttattta aagaaagaga aacttatttt gtaaatatag
     3121 aaaaagttaa atctaacaat atagcttaga gtgtgttttg aagtaaatta atggatagtt
     3181 aaaaagttaa tagttataat attgtatcct ctagaaaaat actagaggtt tttattttgc
     3241 taaaataact aaatagaatt aaatttaaat agtatagttt attttaaatt gaatagttta
     3301 ttgttgatat attttactat tatatatttt tattgtgata taatagtaaa atatattaac
     3361 ctatctttag attatataaa ttattataaa ggagggaaat tttaaaatgt ctaataaaaa
     3421 taaagaaaat ataactttaa gtaatcaaat caaaggaaca attcaaaata gtttaacaaa
     3481 agaagctata ttacatatac caatgagcaa ttatgcctat ggttatgata gagaaacact
     3541 acatataaga cttagaacta aaaaaaatga agcgaaaaga gtaattttac gaataggaga
     3601 ccaatatgtg tgggataaag gaggtgcagg aggaggaaat ttaaatgcat ctggagttgg
     3661 ttggtctggt ggaacaaata tagtaatgag taaagaagta gaaactgaat tatttgacta
     3721 ttggatagct aagtgtaagc ctttaaataa acgctcacga tatggtttta taatagaagg
     3781 tcaagaggaa aaacttttat ttacagaaaa aagaataata gagttgggaa atgaaaatga
     3841 tgaaaaggaa ttatgtgaaa taggtaattt ttttggatat ccatatttaa attatataga
     3901 tatacctaaa gtgccaaatt gggtgaaaga tactgtttgg tatcagatat ttccagatag
     3961 atttgcaaat ggtgacccgt ctataaaccc agaaggagta gataaatggg gagctattcc
     4021 aaccagagat aattttacag gtggagattt acaaggtgtg atagaacact tagattattt
     4081 gagtgatttg ggaataaacg gaatttactt ttgccctatt acaattggaa aaaccaatca
     4141 tagatatgat acggtagatt atatggaggt agaccctaca ttaggagata aagagacttt
     4201 aaaaaaacta atagaagaag cgcataagag aaatataaga ataatgttgg atgctgtttt
     4261 taatcatata ggatattatt ctaaacagtg gcaagatgta gttcaaaata aggaaaattc
     4321 tcgatataaa aactggtttt atataaaaga tatgagtaaa attgatactc caatagaaca
     4381 aatagatgaa aaaaatatac cttatgaaac ctttgggtgt gaaaaatata tgcctaaact
     4441 taatacagaa aattcagaag taataaaata tttattggat gtaggcaagt actggataca
     4501 ggaatttgat atagatgcgt ggagattaga tgtttcaaat gaagtagacc atgttttttg
     4561 gagaaagttt agagaagaag tcaaaaaagt aaaaccagac atttatatat tgggagaaat
     4621 ttggcatggt agtttaccat ggctaatggg tgaccaattt gattcagtta tgaattactt
     4681 gatgtcagaa gccatgaaaa aattcttttg tacagatgag ataaacgccg aagaatttaa
     4741 gtatatgata aatgatgtaa tggtaagtta tccaatacaa gttagtgaag ttatttttaa
     4801 tttacttgga agtcacgata ctacaagaat attgacttat gctaatggaa atattgatag
     4861 atttaaactt gcatatatgt ttatgtttgt acaaacagga tgtccttgta tatactatgg
     4921 tgatgaaatc ggaatggaag gagaattaag ttcaatatct gaaggtcaaa gaaaatgtat
     4981 gaaatggaat gaagaaaaat ggaataagga tatatttgat tttatgaaga agattattag
     5041 acttcgtaaa gaaaataaag aacttaggtc tatatcaaat gaatgggtgt tagcagataa
     5101 ggaaaatgga acgattatat tgaaaaaaga atctatttct ataataataa ataattcatc
     5161 taaaaatata attttgacgt taccagatta tttagcaaat aaaaaagtaa aagatttata
     5221 tgaagaagaa atagttgatt taaaagaaga actaatgtta gagaaataca agttcattat
     5281 tttaaaataa tacaaaaaag tatttatttt agatttcatg ttaaattatt atacagttta
     5341 aataagtctt tataaacgat aaaaaaatat ggtctacaag tgagtgaatt gaaagtagat
     5401 tttaaattaa agactgagtt agagtagata attttataaa taatatctta aatacaagat
     5461 agaaaataaa ttgattatta gtctaaaaat ttcttgtaga aagaggaaat aagaatgatt
     5521 gaagcattta tatttgattt agatggtgta attacagata ctgcatatta tcactatatg
     5581 gcttggagaa aactagccca taaagttggg atagatatag acacaaaatt taatgaatct
     5641 ttaaaaggca taagtagaat ggagtcattg gacaggatat tagaatttgg aaataagaaa
     5701 tattcatttt cagaagaaga aaaagtgaga atggctgaag aaaaaaataa ttactatgta
     5761 tcattgatag atgagataac ttcaaatgat attttgccag gtatagaaag cttgttaatt
     5821 gatgtaaaat ctaataatat aaagataggt ctatcttctg caagtaagaa tgcaataaat
     5881 gtattaaatc atttgggtat aagtgataaa tttgatttta tagctgatgc aggaaaatgt
     5941 aaaaacaata aaccacatcc agagatattt cttatgtcag cgaaagggct taatgtaaat
     6001 ccacaaaatt gcatagggat agaggatgca agtgcaggta tagacgctat aaactcagct
     6061 aatatgtttt ctgttggtgt tggaaattat gaaaacttaa aaaaagcaaa tttagttgta
     6121 gattcaacaa atcaattaaa gtttgaatat atacaagaaa agtataatga gtatatagtt
     6181 aggagaatta tatgaaatgt ttatttagta atgtagatga gtggaagatt atacaagata
     6241 agattgaaat caaagaaaat agattagcag agtccataat gagtataggc aatggatata
     6301 tgggtatgag aggaaactat gaggaaaatt atagtggaga tacacacaag gggtcttata
     6361 tagctggagt atggtttcca gataaaactc gtgttggttg gtggaaaaat gggtatccag
     6421 aatactttgg taaaattcct aattcagtca actatatagg tgtaagaatt ttcatagatg
     6481 atgtagaatt agatttagca aaatgtaaag tagaagattt ttatagagaa ttagatatga
     6541 aaaatggtat tttaaatcgt aggtttattg taaatataaa tgataaaaaa tttgaagtta
     6601 gggttgctag gtttttaagt atatctgtta aggaacttgc agtaattaaa tacagtataa
     6661 aatcattaaa ttctaatact aaaataaaat ttgtaccata tttagattca aatataaaaa
     6721 atgaagattc aaactatgag gaaaagtttt ggaatggaat ttcttcaagt tctttagaat
     6781 ataaagggag tattctttct cgaacaaagg agaatgcatt taatacacca gtattcactg
     6841 tgggttcaat gatgtatatt aagacaaatg gtgatgtaga agctatatct catgagagtg
     6901 attataatta ctgtgaaaat tcgataacaa tggatgtttt aaaaaatgag gttgcaagta
     6961 tagaaaaata tgtatctata acttcaacta gagattataa ggaaagtgag ctattaagaa
     7021 aatgtgaaga cattttaaat agagaactat ctaaaagtta tgatgatata ttaaatgaac
     7081 atacaaagtt atggaataaa agatgggaaa gtgcagatgt aaaaataaat ggagataaaa
     7141 gtagccaaca aggtataaga tataatttat tccaactatt ttcaacatat tatggagaag
     7201 attcgcgatt aaatataggt ccaaaaggat ttactggtga aaagtatgga ggagctactt
     7261 attgggatac agaagcattt tgtgttccag tatatctagg tgtagcagat aaggaggtaa
     7321 ctagaaatct tttagaatat aggtacaatc agttagaaca ggcaaaggat aatgcaaaaa
     7381 aactagggct taaaggtgct ttatatccaa tggtaacatt tgatggagta gaatgtcata
     7441 atgaatggga gataacattt gaggaaatcc accgaaatgg aagtatagta tatgcaatat
     7501 ataactatac aaattataca ggtgactata aatatataaa agaaaaagga atagatgtaa
     7561 taatagaaat agctagattt tgggcaagta gagttcattt gtctacaaaa aaagatttgt
     7621 atatgataca tggagtaaca ggtccaaacg agtatgaaaa caatatcaat aataattggt
     7681 atactaatta tatagctaaa tggtgtttag attatgctgt agaaaatatc ttacgattag
     7741 agaaagagtg taatgaatgt attgacagaa ataatgtgac tagggaagaa gttgagtttt
     7801 ggaaaataat atcaaataaa atgtatctgc cgtatgatga ggaactacaa ataataattc
     7861 aaaatgataa ttttttagat aaagaatttg tagaagtaaa ggatttacca aaagaaaatt
     7921 ttcctcttaa tcaaaattgg tcttgggata agattcttag gtcgtgtttt ataaaacaag
     7981 cagatgtatt acagggaatt tactattttg gaaatagatt tactaaagaa gaaaaaaaga
     8041 gaaattttga cttctatgaa aaatatactg tacatgaatc ttctttatct tctagtattt
     8101 attctattat tgcctcagaa atagataacc tagaaaaggc ttatgaatta tattcaagaa
     8161 cagctaggct tgatttagat aattacaata atgatacaaa tgatggactg cacataactt
     8221 caatgtctgg ttcatggctt tccattgtac atggatttgc aggcatgaga acatggaatg
     8281 aaactctatc ttttgaccct aagttgccaa aagagtggaa aaattattct ttcaatataa
     8341 attatagaga taatattatt aatgtagatg tatgtagtga tttaattaaa atagaaaatt
     8401 taaaaggtag accaataaaa attattgtat atgggaaaaa atatatatta gaaaaatgta
     8461 taaaaattga taaatagatt taataatatt tttattatat aaatagttaa aataagctat
     8521 ctaattattt atttatttta actattttgt gtgatagaaa actatatatt ataacaatag
     8581 tagtgctttt aattaaatat atttcataaa tcttcataaa atcttcaaaa tttcgtaaag
     8641 aatatgtcac aaaagtaagg tatattatat tcagcagata gtataaggga ggacaatatt
     8701 tatgaagaat aaaaaattca ttgttataat actgataata tcaattttaa taggtgtatt
     8761 agtgaaggaa tattcatcta gagagataaa aaaggatgat ataaatgtca gtaagtatat
     8821 aaaatatgct gatttagcaa gtaaaaataa tgcacaagtt aactggaagt atgtagcttc
     8881 cattgttgct gtgttaaata aaaataactt aaaaaacgtt aaggattcac agatacaaga
     8941 agtatcagat ttatttgtta agaatttttc taaaaataat aaaataaata agttaagtga
     9001 tattttggat gagttagaat ttagtaatag acaaaaaaga ttggttgata attatataga
     9061 caatcttaag gattatggta taaaaccaga aagattgaag tctgatacaa aatatatgaa
     9121 gtttattgat gaaattaaaa cagaagcaat acaaaattat aaggattata aaatacttcc
     9181 atctataact attgcacaag ctataataga atctagctgg ggcaagtcaa ctttggcaaa
     9241 acaatatagc aatctatttg gtataaaggc tgacgcttat tggaaaggta agtcagtgac
     9301 tttagaaacg aaagaacatt tagatacaat tatagatgat aaatttagaa tatatgatga
     9361 taaaaatgaa tctataaaag accatgcaaa atttttagcc acaaataaaa ggtataaaaa
     9421 caatggtgta tttgatgcaa aaacgtatat atatcaagct aaagcattag aaaaggcagg
     9481 ttatagtact gcgaaggatg aaaatggcaa tagtatatat gcagctcgtc taatagaatt
     9541 aattcaacag tataatcttc aattaataga cagtgaaatt caaagtgaaa tgtaactttg
     9601 attaaatttt tattgataag gctatgattt ttaaaatcat agccttattt tgacatgctt
     9661 ttacttgtaa ttattaaatc gatatcatta tctttcaaaa tttactttat actgtaaatc
     9721 aaattattta agttaaaact tggaattatt tataaaagga ttatttactt tatctaaatt
     9781 ctgcacttaa agaacatctt ttcccttaag actgaccgtt agtctataaa aattataatt
     9841 acttataaaa aagtgaatat atatataaag ttttcagaaa gacttttact tgtaaattga
     9901 aatcaatgaa gtaaaaaagg attctttctt aaaagtaaat aacaactaga tagtgaataa
     9961 taaaaaatgg taatttgaaa taatttttat gaatagtatt aatatataga tacagggaga
    10021 ttgatgcgat tatgtatgaa aataataagg taatatacaa ccttgaaaga tgtgaaaaac
    10081 tagaaaagac tctttattta tctcaattta atacatataa aaattttaaa ttagcttcta
    10141 aaaaaactgt aataaattca ttagagacat atgtttacaa tatgaatatt aacaatataa
    10201 gtcttgttga tactatagaa tgtatatcat tagaatatga acaacttaca ggaaagaagt
    10261 tgattttagt tggtgatata gatattgatg caatattaga ttatgcagga aataaaagaa
    10321 atagaaattc tagtaaaaaa aatacattta aattaaaaat accctttagt acatttatac
    10381 aaatgcctag aaagatagaa aataaagata aaattaattt aaagtatctt atacaagata
    10441 ttacttcatc tattttagat gataatttat ttattagtgt tactgcaatt ataagttatg
    10501 aaaatagttc caagatagag taatctttaa attatagaaa ggtggatgca atgagaataa
    10561 cgaaaactac ttttaatgat gtattttcaa atgatgcaaa tggaagtatt ataaaagtag
    10621 aaaaatatgt tgataaaaaa tgtgcatctg tatttgagat tttgacctac actattattg
    10681 ttacaaatac aagtaggtac aaaactggaa atatattttt caaagattat atttctaaat
    10741 atatagagtt tataaataat actgtaaaag ttaatggcat tataaaacgt ggattagacc
    10801 cacaacaagg tttttacata gggagaatag atgcaagttg taaaaaaata atatctttta
    10861 agagtgtagt tttaccaaat tctgcacata ggattataaa gaatagtgct aatatatact
    10921 attattataa gtgtaatc
//
LOCUS       DAELNY010000123        10923 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.123, whole genome shotgun sequence.
ACCESSION   DAELNY010000123 DAELNY010000000
VERSION     DAELNY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.123"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            340..4482
                     /locus_tag="KPE55_002648"
     CDS             340..4482
                     /locus_tag="KPE55_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG0493698.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFTENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTTQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
     gene            4937..5284
                     /locus_tag="KPE55_002649"
     CDS             4937..5284
                     /locus_tag="KPE55_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0493699.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(5434..5934)
                     /locus_tag="KPE55_002650"
                     /pseudo
     CDS             complement(5434..5934)
                     /locus_tag="KPE55_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5961..7466)
                     /locus_tag="KPE55_002651"
     CDS             complement(5961..7466)
                     /locus_tag="KPE55_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0493700.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLTNLNKELDTLEKQKE
                     RLHDFLEQGIYDNNTFGERLKILLSRIENVREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(7665..8519)
                     /locus_tag="KPE55_002652"
     CDS             complement(7665..8519)
                     /locus_tag="KPE55_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0493701.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            8904..9185
                     /locus_tag="KPE55_002653"
     CDS             8904..9185
                     /locus_tag="KPE55_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493702.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            9335..10183
                     /locus_tag="KPE55_002654"
     CDS             9335..10183
                     /locus_tag="KPE55_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBG0493703.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            10235..10756
                     /locus_tag="KPE55_002655"
     CDS             10235..10756
                     /locus_tag="KPE55_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493704.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
BASE COUNT         4008 a         1553 c         1620 g         3742 t
ORIGIN      
        1 taaaaagagt aaaaaataac aaaaaaatca ctgacatttc actgacaaat tgtaattttg
       61 ttattataaa tacattgaaa atacttgatt attgaaaaat ggcataaaat aatacatttt
      121 atgccatttg ctattatttg atagttttta acttatgaca taagatattt tttaacatat
      181 acaataaaat ataaaaaaat tgatattgca aataaaaaaa aatacatatt agatgttata
      241 gaaaaaaagc gcacttatag gcttaaaatg tcacataaaa gagttttaaa gaataatata
      301 ttatgaacac aaattttgta atcacaaagg agtgataata tggcaacagt atcaggacaa
      361 atagtttttg atagaaatag aagtgctacc attgattctg gagactcagg aatagccaat
      421 ataccagttg tattacaaga tatagcaact ggagttagac ttgtggttct cacagatgca
      481 tcaggaaatt atgtttttac taatgttcca aatggtaact atagaattgt agaagctttt
      541 ggaactacag gaggagtacc aacaccagga aattttgcaa cagcgatagt aggtccagtt
      601 ccaattgcag aaatgccacc tattagttct gtgacaagtc ctcctattgg ttcaacaaac
      661 ttagatggta caacacctaa cacactatta ataacagtag caggttcaga tataacaaat
      721 caaaatatat taaatgggcc tgtgatttat acacctatag agaatatatt ggattcttgc
      781 gtatctgtat ctaatataaa ccttattaca gatgcagata acggaacatt cggttttttt
      841 ccaccaggta caccaagtaa tacaggacct gaaatagaac cttacccaaa tgtgacgcca
      901 gattttacgt atgttgtacc agacccaact aagtttacac ctttagatgg agaatataca
      961 gtacaaaata ttatgacaaa tgcaatgagt aatgttatag gagcttggtg gcgtattgct
     1021 gaccatacta caggaaatga aacaggaaga atgatggtgg taaatggttt taatcctgga
     1081 gctgtatttt ttaaatcaac agtacaagtt acacctaaca ctaattattt atttagcact
     1141 tggattttaa acctttttaa ggtaacagga tatccaccac cacagctagg tgtaagaata
     1201 tttgaccaaa ataataatat actatatcaa gcaacattag gagctcaaat tccagttaat
     1261 acaaacgcac cagaatggaa agaagtaggt actgtgatta attctcaaaa taataatcaa
     1321 cttacagtag aattttttag tgaaggagaa agcgccattg gaaatgacta tgctatagat
     1381 gatgtttctt taagagaaat agctgttcca acttttactc caataaaaac aagtaacaca
     1441 gaaactgcta atgtaggtga tataatcaca tatacagtta cattagataa tacttgtgat
     1501 agccctttaa ctaatgtatt ttttagggat ttaattccaa atggtttaga gtttatacct
     1561 ggaactgtaa cggttgatgg agtatctgta ccaggagtag accccaatac aggtttccca
     1621 cttccagata ttggaggagg aacaggggtt gaagtaactt ttgatgttgt ggttgaaagt
     1681 attcctaacc caaatcctac taataatata gctactatag attactccta tacacctgta
     1741 gaaggcggta taccaaatga tttttcagta gattctaatc cagttcctgt tgaagtaatc
     1801 tcagcagata ttgaagttac aaaattatct gaacctacta tagtgaatcc aggagaggaa
     1861 ttaatataca ctataaaagt agtaaataat ggaccattcc catctgaaaa tgtggtttta
     1921 acagatgatg taccagcttc tattgtaaat ccagagtatt cattagatgg aggagtaact
     1981 tttcaaccat ggactggaag tttaaatata gggacattag aagtaggaga gacaagagtt
     2041 attataatta ggggtattgt aaatccatca acagtaggaa ttataacaaa tacagctgtg
     2101 gtatcttcaa ctacagcaga cccaaacctt aataataata catctacaat agagacagaa
     2161 gtgaatttat tagcagatat tttagtaatg aaaacagcag aacctaattc agctgttcca
     2221 ggtactttat tgagatatac tattcaagta gaaaacttag gaccagcaaa tgctgaaaat
     2281 gtaatattaa atgatgatat tccagcttct attataaatc cagaatattc attggatgga
     2341 ggagcaagtt ttcaaccatg gaatggaagt cttaatatag gaactttaaa ctctggaatt
     2401 tcattaacag ttttaattca aggaacagtt agtttaaatt cttcagaata tatagtaaat
     2461 acagcaacag tttcttcaac tacacctgac ccagatttaa gcaacaatat atctaccatt
     2521 attacaccag ttaacccaca agcaggtatt tctattataa aagtagctga cgaggatgta
     2581 gctgttccag gagaagaatt tgtatataca attgaaattt ttaatgaagg accatctaat
     2641 gctacgaatg tagttttaac agatgatata ccagatgtta ttttaaatcc agaatattca
     2701 ttggatggag gagcaacttt ccaaccatgg actggaagtc ttaatatagg tacagttgca
     2761 ccagggcaat taataagaat tatcataaag ggattagtaa gttcaactgc tacaggagat
     2821 attactaata cagcagaagt aagtgcagat gtaccagaac cagttacgga ttcatcaaca
     2881 gtgacaactc ctatagttcc atcagcagat atagaagtaa taaaaactag caatatggat
     2941 acagcagttc caggagaaac tttcagttat actataacag ttacaaattt aggaccatct
     3001 gctgcacaaa gtatcgtttt gacagatgat ataccagatg ttattttaaa tccagagtat
     3061 tcattggatg gaggagtaac tttccaacca tggaatggaa atcttagtat aggcactttg
     3121 gatgctggag aaataagaag tattataata agaggaacag tgagccagac agcagtaggt
     3181 acaattataa atacagcaac aataagttca cctacacctg acccaaatcc agataataat
     3241 acttcaacag atgaaacaga tataagtcca ttagcagata tttctgtgat aaaaggcaac
     3301 gaaccagttg ctataccagg gggaagattt atttatggaa ttgaaattgc aaatgcagga
     3361 ccatctttta cagagaacgt aactttaaca gataatatac cagcttctat tttaaatcca
     3421 gagtactcaa tagataatgg agtgactttc caaccatgga atggaagcct taatataggt
     3481 actttagatg ctggagagat aagaaatatc ataataaggg gaacagtaag tcaaacagca
     3541 ataggcacaa ttataaatac agcaacagta agttcaacta cacctgaccc aaatctaaat
     3601 aacaatactt caacatcaga agcagaggta agttcatcag cagatatttc tgtagtaaaa
     3661 cgaagcaatc agattgttgt tgtaccagga gatgtactag actatacaat tgaggtgaga
     3721 aatgcaggac cgtctactac tcagaacgta actttaacag ataatatacc agcttctatt
     3781 ttaagtccag aatattcaat agataatgga gtgacttttc aaccatggaa tggaagtctt
     3841 agtataggca ctttagatgc tggagagata agaaatatta taataagggg aatagtaagt
     3901 caaacagcaa taggtacaat tataaataca gcaacagtaa gttcaactac acctgaccca
     3961 aatccagata ataatacttc aacatcagaa atagatataa gttcatcagc agatatttct
     4021 gtaataaaat tagcaaataa aacagaagca tgtgtaggag agcaaataga ttttgtaata
     4081 gtagtatcaa atgcaggacc agaaaatgct caaaatgtta ctttaataga caatgtttca
     4141 gataagctta agaaagcagt attttcactt gatagaggtg taagtttcca accatggact
     4201 ggaagtctta atataggtac actaccagct ggaactttaa gagttattct attaagagga
     4261 attataaaat caacatgtct tgatagatta acaaatattg cagaaattac ttcaacaaca
     4321 ccagattcaa accttaacaa taatatatca agagtgcaag tagaaataaa acaatgttgt
     4381 tgtaatgatt gctgttgtaa caactgtagt aattgttgtt gcaatgattg tggttgtaaa
     4441 gattgttgct gtaattgttg taaaaatgac ttttggtgtt gattttataa aataattttg
     4501 tataccttaa attaaactga aataaaaaat aaattcacat atgagtaaaa tattttataa
     4561 atattaataa attttctatc tttgatatgg accacattcg taatactagt tattaaatga
     4621 agtggtcctt tttaattcat taaaattatt tattctaatt aaattttaaa tgtattaaat
     4681 aattttaatt ttttataaaa aatattcata tgttatttat attaaagttt agatatctaa
     4741 aaatactttt agaatttatt atattatgtt aattttttat atcttataag aattgtattt
     4801 ttctaactat aaaaatagta ttatacttat atttaaaaat aaaagaattt tattcctatt
     4861 gtgtaaagtg agaatactat tttcttatat tggtataata atagtaaatc taatatagaa
     4921 aagaggtatt tctagtatgc atgaaaataa aaagaatcta ttagacggaa gcttagctca
     4981 atataatgat acagcagttc caaaaatacc agtatttgct ggtaacgata taacttcaga
     5041 agtttattat tttaaaccta atcaagttct taatgctcat agacacccta atggtgaaca
     5101 aatatttgtg ttcctaaaag gagaaggtaa aatgaaactt ggtgagcatg aatgtgatgt
     5161 aaaaaacggc gatactgtat ttgtaccaac aggagagtgg catgaaatta ctaatggaag
     5221 caatgaagaa atggtagccg ttcaaattac aaaaattaat gctggagcag aatacagagg
     5281 atagatatat aagtacagtt agtactttaa tatctatttt attatataaa ctttaatctc
     5341 aaatataaaa agagtctttt aaaaggctct ttttatattt ttgttaattt tcattataaa
     5401 aacaagaaaa tatctagcta aaactaatat attttattta atatttttta tagcattgtt
     5461 tgcaaactca gtatttacga tatctttata aggtgcttcc ttatctagtt ctttagcttc
     5521 tttcataaca gtcatcaatt tcttcaaacc ttcctctttt aatacaggat tttgagacca
     5581 tgcatctatt gatttatatc tatctacaac tgttgttaaa tcttctaaag atatatcttc
     5641 aaagaaaggt tgcatagcct tagctacttc tttgctagat gcttgttgaa tccataattg
     5701 acctttataa acagcatttg taaatctttg aattaaatct ttattgtttt tcatgtatga
     5761 tctcgtagta gagtatgctg taaaaggtat ttctccagaa tcttttccta tagatgtgac
     5821 tatatatcca ctaccttctt tttccattgc tgttgctgta ggttcaaatg ctgttgtaaa
     5881 gtcaccctct ccacctgcaa aagctccagc cattacacca aattggtaat cagtctattt
     5941 ataacattta aaacatataa ttaatctctt gggatattag ggtaaagtat taaagaaaaa
     6001 tcatcagatt tttgattagg ttctttttta tagactgcat aatttattac acttttaagc
     6061 aatatatttt ttagataagg ttctttttcc tctgtatatt cacttaaaat tttttcaagt
     6121 tgaggaacaa tttgtgtttt aactgtattt ctttcctttt gcatttcaat atctatattt
     6181 gttttattaa tagcttctct tacattttct atacgagata agagtatttt taaacgttcc
     6241 ccaaatgtat tattgtcata aattccttgt tctaaaaaat catgtagtcg ttctttttgt
     6301 ttttctaaag tatccaattc tttatttaaa tttgtcaagg tatgttctaa agtattttca
     6361 ggtttagagt tatctttttc atcttttaaa gatagtctat aatttactaa ccatttattt
     6421 aagacctcta atattttatt ttctaatatg tatagtttag tagctttatt tttacaatta
     6481 gtacaaacca tatatttctc atatccattt gatgtagtat tagatttggc tattaaagta
     6541 tgattacagt atccacattt tacaattcca gccaatggat ttgttatttt attgtttgga
     6601 gtaggggaat taatacgttt ttttcttata ttttgtgcct tattccatat gtcttcactt
     6661 attattggtt catgctttcc ttttacctca atttgttcct caattcctct tgttctacta
     6721 gaatttcttt tcctctcgac tttattccat actatatatc cacaatacac tttgtttttt
     6781 ataatatctc ttataccctt ttcataccaa gttctacctg ttgtggtctt ataaccaagt
     6841 tcgtttaaat aattggatat tttacatgca ccatctcctt ttaaatacat atcaaagatt
     6901 attttaacaa cagttgcttt atcttcattt attacaagta ctctttcttt attatttaaa
     6961 tatttaatat catacccata aggaggagct tgagctataa atttaccctc ttcaatactt
     7021 ttgatttttc ctctttgcat acgtctagat attaatttta attccttacg agccataaaa
     7081 gcttcaaatt cagaatactc ctcatcaaac tcatcattta aatcatacgt ttttctagga
     7141 gtaataattt tcgttttaga ttttttaaat gtatctagta ttaacccttg gtcttgcata
     7201 ttaccacgac ccaaacggtc aatatccatc actaaaacag catcatagtt attattttta
     7261 atttcaccta gtaattctag catcttaggt ctatattcta tactttcccc agaaacaagt
     7321 tcttctttaa tttcaatgac atttaaattt tgttctcttg ctagttttaa aagagttgtc
     7381 ttatgtctac ttagagtttc aaattcacct ctatgttctg cttcttcatc agctctgctt
     7441 ttgcgtaaat aaattgccac attcattaaa tcacacccct tgaatatatt atattcagta
     7501 acagattgat tatatttaaa acacaaaaat aaaccttcaa aatacaagat atatttttta
     7561 ggtttatttt tatacagtaa attttatagt atatttaaaa gcttgtaatc taagtaaata
     7621 tttatactat taattttatg ctttcttcat tttttaattt taaactattt ttttactaca
     7681 ggtatccata cttcaaattt agcattttga gggtctggat ttaagtacag ttcaatatca
     7741 ggaccattat catattcata accagaagta ggtaaccatt ctgttacaat acgcttttct
     7801 agttcttgta ttgcttgtgg acattgtcct tctccagaaa atattgccca agtaaaagct
     7861 ggaacagtgt attcttctaa tgtattatct atagattttg tacttgaaac agatataaaa
     7921 tacttccatt cttctgaatt attacaaata cttataccta atacaccttg aagttgacta
     7981 tccatcatag ggattaattt ttgaagcgtt ccatctaaag caaccttttg ccacatttgg
     8041 ggaacaattt caaaattctt ttctagttca ttatgaagtg gctgagatac accaactata
     8101 cgaaatgctt ctttcttttc aattctataa ttcatttctt cggctccttt aatactaatt
     8161 ttaaaactga ttggaggaaa ggcttttaaa gttgttattt ctcttttttt tataactgat
     8221 ggggcaacac catgaatact tttaaatgca cggttgaatg ctgttggaga agagtatcca
     8281 tattttaaag ccacatcaat tatcttttta tcatcatttt gtaattctac agctgctaaa
     8341 gacatccgtc tacgacgaat atattcagaa agtggtacat ctgccatata agcaaacatt
     8401 ctttgaaaat gatatgtaga acaacaagct atttttgcca cttgttcata ctcaatttct
     8461 tttgttatgt tttcttcaat atagttaatt gctttgttta atctttcaat ccattccata
     8521 aaatcagctc cttgtaattt atataatatc tattttaaaa tagttaatcc tctctttcta
     8581 tgcactaatt aactagctta ataaagaatg aacatgagaa agtctgtaat taactacaaa
     8641 aatgaacaaa tagtattgtt tttcacatta aagtatatca cagattagta tctaattttt
     8701 atataagata acagttttaa tttattatga aatttttata atattttagc ttagacattt
     8761 aattatatat aacattttat tcatagttaa ctatttttga agtttaccct taatcaagca
     8821 acacttagtt actcaattat aaataatgat tgagaatagg acaattttta agatacataa
     8881 aatataaaga ggaggggatt taaatgccaa atatagaaaa agacttagat ggtacaacta
     8941 ctataacata cctcaatgac gatataggtg ttccaacaga cgagaatgga tataaaatat
     9001 ctatgacaat actacatgga aagacaatct gccatgtagt atctcctgaa acaatcttag
     9061 gaagaaagtt cacattagaa gaaaaacaag aaaaagatac aataatatat ggagcaatag
     9121 aaaaaataat ggtagacaga caattacaaa taaaaaattc aaacaaagaa gttgctagtc
     9181 attagcactt ctttaataag taaagtagga caagtatttt ttataatatt attgacttat
     9241 agtctaatta gactataata taaacataga tagtctaatt agactactat taaaaataaa
     9301 tacaataaga atttaagaaa ggataataat aataatgatt aaatcatttt taatgttagg
     9361 acagtccaat atggctggaa gaggattcat aagtgaagtg actccaattt ataatgaaag
     9421 aatacagatg ttacgtaatg gaagatggca aatgatgaca gaacctatta attatgacag
     9481 accagtttct ggggtaagtc ttgctgcatc atttgcagat gcatggtgct gtgaaaatca
     9541 agaggataga attgggctaa ttccttgtgc tgagggaggc agttccttag atgaatggaa
     9601 tatagatggt atacttttta aacatgctat aagtgaagct aaatttgcca tacaaagcag
     9661 tgaattgaca ggaattttat ggcatcaggg agaaaatgac agtaataatg gcaactataa
     9721 attttattat aaaaaattac tttctattat tgaaactctt agaaaagaat taaatattcc
     9781 agatatacca attattattg gtggattggg agacttctta ggaaaagtag gttttggaaa
     9841 aagttgtact gagtatgtat ttatcaatca agagttacag aaatttgctt ttgaacaaga
     9901 taattgttac tttgttacag caacaggtct tacatctaat cctgatggta ttcatattga
     9961 tgcaatatct caaagaaaat ttggattacg ttattttgaa gcttttcata agaaaaagca
    10021 tattatggaa gcattagcta atgaaagtga gttgataata ccgagtaatt ccaagacata
    10081 tacaaaaaca gaaaaaatat atataaatag tatggacttg gcactaggaa agatatcata
    10141 tgatgagttt gaatctaaac ttattaaact taatgataat tagataaaat attcattaat
    10201 atactaataa atatagattg aaagaggtga ttttttgata ccattattaa ttttaggatt
    10261 attaaaacaa aatccaaatt cttatggata tgagttatta gcattaatgg aaaagaagca
    10321 ttacaagtac atagtaaatt tcactaaagg ttctttttat tataatttac agcaacttga
    10381 agaaaaaaag tttattgaaa aagttgaaaa taaacaatct aataaaaaca gagaaactca
    10441 taactacata attactgaac ttggatgcca ggaatttgaa aaattaatgt ataagtatgg
    10501 ttctaaaaca gagtatataa atctatcatt ttacgcaaca ctattatttg caaatgatta
    10561 taaaaatgaa aagttaatag aattattgga aacgcaaatt aagcaaactc aagtaagaat
    10621 ttctctatta aatgaatcca tagaaaatga caaaacaaat tcacattatt ttaatagaat
    10681 gctagaaaat tctcgttctc accatttagt taatattgaa tggtttgaga agttgctaaa
    10741 tgatattaga gattgatttt aataataaaa aaacatacat atatacttta tataaatgta
    10801 tgatatatag attaaaaata aggcagataa ttttcaattg aatatctgtc tttttgttat
    10861 ataagatgtt aaaagacact tatactatgt aagtaaaatt tataaatata aagttgttca
    10921 ttg
//
LOCUS       DAELNY010000124        10803 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.124, whole genome shotgun sequence.
ACCESSION   DAELNY010000124 DAELNY010000000
VERSION     DAELNY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.124"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1053)
                     /locus_tag="KPE55_002656"
     CDS             complement(<1..1053)
                     /locus_tag="KPE55_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG0493705.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNK"
     gene            1120..1491
                     /locus_tag="KPE55_002657"
     CDS             1120..1491
                     /locus_tag="KPE55_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG0493706.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1528..2202
                     /locus_tag="KPE55_002658"
     CDS             1528..2202
                     /locus_tag="KPE55_002658"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG0493707.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2451..3290
                     /locus_tag="KPE55_002659"
     CDS             2451..3290
                     /locus_tag="KPE55_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0493708.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3534..3630
                     /locus_tag="KPE55_002660"
     tRNA            3534..3630
                     /locus_tag="KPE55_002660"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3568..3570,aa:Sec,seq:tca)
     gene            3781..4488
                     /locus_tag="KPE55_002661"
     CDS             3781..4488
                     /locus_tag="KPE55_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG0493709.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4630..5466)
                     /locus_tag="KPE55_002662"
     CDS             complement(4630..5466)
                     /locus_tag="KPE55_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493710.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5654..6559
                     /locus_tag="KPE55_002663"
     CDS             5654..6559
                     /locus_tag="KPE55_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG0493711.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6568..7752
                     /locus_tag="KPE55_002664"
     CDS             6568..7752
                     /locus_tag="KPE55_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0493712.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7867..9168
                     /locus_tag="KPE55_002665"
     CDS             7867..9168
                     /locus_tag="KPE55_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG0493713.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9122..10165
                     /gene="holA"
                     /locus_tag="KPE55_002666"
     CDS             9122..10165
                     /gene="holA"
                     /locus_tag="KPE55_002666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG0493714.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10352..10618)
                     /gene="rpsT"
                     /locus_tag="KPE55_002667"
     CDS             complement(10352..10618)
                     /gene="rpsT"
                     /locus_tag="KPE55_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG0493715.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         4229 a         1115 c         1674 g         3785 t
ORIGIN      
        1 tttatttaat ttttcaatag aaaaagattt atctgtaagt ttaaaattat cttttatacc
       61 cttagttaag tatactttct tatccttagt tatgaatata gcatctacat tatcaatttt
      121 attaattaaa ttcatacctt tttcaagacc aagaccaaat gtagaagtgg ataaggcatc
      181 acaattaata gatttatcag agattattgt aacactactt aaattgtttt caaaaggata
      241 ccctgtaaat ggatttaaca tatgatgata tatttttccg tctttttcga taaatctttc
      301 ataaattcca gaagtaacaa cagacttatt tgtagtttct atgtttccta tggattcacc
      361 atttttacta gttgggtctt gaatacctat tttaaaaggt ttatcatttt taccattttc
      421 taaagtaaat atatttcctc ctaagtttac taaacctttt tttacatcct ctgattttaa
      481 ataggctaca attttatcag cagcatatcc tttagctatg gcacctaaat caattttcat
      541 attttctttg gttaatttta tagatttatt tttttcatcc aatatgatgt tcttataatc
      601 aataagaggc agaagttttt gtatcttaga atcacttgga actttagctt tatctgttcc
      661 aatagcccat aaatttgata atggaccaat tgttatgtca aaatatccat ctgataattt
      721 ggaatattgt atagcttctt ttacgacgaa aaatgtatca tcagaaactc tgacaaattt
      781 tttaccagca ttatcattta ttttagaaac atcacttcct ggtatatgag tgctcatttt
      841 gttgtcaata tatcttaaaa ttgaatcaca ttcatttaag attttatcac ttttggattc
      901 ttttacatta aatacagtaa cttcgtttac tgtaccaagg tagtagctag ttttactata
      961 tttagtattc tggcttgaac tcctaaaaaa tgaaaataaa attaatgcta aaatcaatat
     1021 cacaaaaata aatgtgattt ttttcttatt catggtatat tcctcctatg aaattgtgtt
     1081 attattttat tataacataa tttcatagga ggacaaaaaa tgaagaaaaa agacattctt
     1141 cttattttag gcattttggt tgttattaca gcatgttatg ggataataaa tgttataaat
     1201 tctaaaaatg ctggaaatat tgagatttat gtagataata aattgtataa aactgtttca
     1261 ataaatgcca aagaagaatt taaaatagag agtagagggg ggtataacat agtaaagatt
     1321 catgataaag gggttgagat agtagatgct tcatgccctg ataaggtttg cgtacatact
     1381 ggatttataa acaagccaag tcaaagcata gtttgtatac ctaatagagt gagcataaaa
     1441 ataaatacaa atgaaaagaa tgataatcaa gaagatataa tttctaaata aaataataaa
     1501 aataattgaa aggaattggt gataattatg gttaacttag gaaacgattg ggatgaatta
     1561 ttgaaagaag aatttgaaaa agattactac ttaaacttaa gaaaatttct tatagaagaa
     1621 tataaaacac gccaaatttt cccaaatatg cacaatatat atgaagcttt aaaacacact
     1681 tcatacaaag acacaaaagt cttaatatta ggacaagacc cttatcatgg agataatcaa
     1741 gctcatggtt tagctttttc agttcaacca caagtgaaaa ctccaccatc tttattaaat
     1801 atgtataaag aattaaaaga tgatttaggt tgttttatac ctaataatgg atatttaatg
     1861 ccttgggcag accaaggagt gttactttta aatactgctc ttactgttag agcgcatgaa
     1921 gctaattctc ataagaacaa aggatgggaa attttcactg atagagtaat atctatttta
     1981 agtgagcgtg aagacccagt tatttttgta ctatggggaa gtaatgcacg taaaaaagta
     2041 gagttgattg atacaagtaa acattatatt ttggaagcac ctcatccaag cccattgtca
     2101 gcaagtaaag gcttttttgg atgtaaacac ttctctaaga taaatgaaat tttgaaaaag
     2161 ctaggaaaag aacctattaa ttggcagata gaaaatatat aaaagtaaaa ggtaacaaaa
     2221 tgataacaaa acaacaataa acaatgacat tgttgtaact aatagaaata tgcttaaata
     2281 gttgaaaata ctatgtatgc atatttctat ttttttgtca agttgacaac tcttgaaaaa
     2341 atagttacaa tttagttaca caaaaaacac aaataaaaaa agaaacttac aaagcagaat
     2401 tttatgaagc aaaatatata gtgtaaaaaa tcttagggag gaaattttat atgaatttta
     2461 aaaaattgag taatgctaaa gtctcggtaa tggcagttgt tttatcagtt ggaatattat
     2521 cgggatactc tgcgcttaat aaacaagtaa cattagtagt aaggggagaa gaaagaacta
     2581 tatcaacatt tagttctaat gtaggagacc ttttagaatc tcaaaacatt agctatgatt
     2641 caaatgatat agtaacttta gatgaaaata caaagttatc taatggagat aaaatagaag
     2701 taattgatgt aaaggaaaaa acagttgtag aaactaaaga cattccattt gaagttacag
     2761 ttgttgaaga tggctcttta ttaaagggtg attctaaggt taaagaagaa ggaaagtctg
     2821 gtaaaagtga attaatatat aaagaaactt atcacaatgg aaagaaagta gaaaaggaat
     2881 ttgtgaaaaa ggttgttaaa gctgagccag taaataaggt tgtaagcaaa gggacaaagg
     2941 tagaaatgca agtagcctca tcaaggggag aaagttcaag aagaatggct tcatcatcat
     3001 ctagtagcag tagtagtaac aatggtaaaa atatgagagt ggtagcaact gcatatactg
     3061 gtcacagtat aactgcaaca ggaacaaaac ctaagtgggg aacaatagct gtagacccta
     3121 aggttatacc atatggaact aaagtttata tacctcaatt tgacatggtg tttactgcag
     3181 aagactgtgg tggagcaata aaaggaaata aaattgatat ctacatgaat gattcaaaaa
     3241 cagtttataa ctggggaaga aaaactatag atatacgtat attaaaatag ttaaataatt
     3301 tatattttta ctggaaatta tattttgatt atatagagaa gaaactcttt atagattcta
     3361 aaaatataat ttccagtttt ttattttgga tatgtaataa aacaggtaaa ttttaaccat
     3421 gtatagataa aagctattta aattataatt tactatcata taaatctata aaaattgttg
     3481 caaagagtca aaatatatga tattatagat ttcgttgaga aggatttcat tatgggagtg
     3541 gatgggtgcc ggtgtgccct ctagtcttca aaactagtat gaggggttaa gagcctctta
     3601 ggtgggttcg attcccacgc attcccgcca atatgtaaaa ggtatctttt aattaaaaga
     3661 taccttttta aatttatttt aagataattt atttttaata atatataatg ttttgtatta
     3721 tataatgtaa attttctaat actattatat atttagataa aaatctagga gtgtggaaag
     3781 atggaaaata attttacaac gagaactagt tttctagttg gagatgatgg gataaaaaaa
     3841 cttaataatt caaatataat agtatttgga gttggaggag ttggtagttt tactgttgaa
     3901 gctttagcaa gagctggagt aggcaatatt actattgtag attttgatga tgtagatata
     3961 accaatataa atagacaaat accagcccta cattcaactg taggaagata caaagttgat
     4021 gtcatggaag aaagaatatt agatataaat cctaatatca atataaaaaa aataagaagt
     4081 ctttataata aagatactag tgacgaaata ctaactgaaa gatatgatta tgttgtggat
     4141 gcgatagaca tggtaagttc caaaatacat ctaattgaaa cttgtgaaaa aaaaggattg
     4201 aaaataataa gttctatggg tatgggaaat aaattagacc caactaaaat agtcgttaca
     4261 gatatacaca aaacaagtac ttgtcctcta gctaaagtta tgaggaaaga acttagggat
     4321 agaggtatca aaaagttaaa agtagtatat tctactgaac aaccaataga acttaagaag
     4381 aaagtaatga atggtagaaa agtaacacct ggtagtgtat catttgtccc atctgtagga
     4441 ggtttaataa tcgcatctgt aatagtaaat gaactattag gacaataagt tttaaatatt
     4501 ttaatttata aattttatat aaaaatttga aggctagaag catgaataca aagcaatttt
     4561 atattcatac ttttagccta attattttat attaccttat tttatgtagt tgtagtttta
     4621 ttttttagat tatgtatcta tttcataatt atattttgta aatataattt ctccatcact
     4681 ataaaaaaca atatctccta ttctatctgt tctataaatt gatacatggt tttgttttag
     4741 attattaata acctccctat gtggatgacc atattgattt ttatatccac atgatattgc
     4801 agctatatct ggactaattt tttttataaa ttctaaagat gaagaagaat tacttccatg
     4861 atgacctatt tttataaaat taatattatt taatttaaaa gaatgtaaaa tttctttttc
     4921 attttcttct tcagcatcac ccataaataa aaaagaatta tcattaaaag ttaatttgaa
     4981 aactatagaa tttaaattag attcttcttg tatatatgat ggacttaata catatatatt
     5041 tatattgtta tctatgttta aaacatcatt tttatagaga tgctgaatag ataagttttt
     5101 atccgtacaa gaatttatta gattttgata agccttacta tcagttgatt gttcaggcat
     5161 atatatggag tttacattaa atttttttat aatattatca agacttccta tgtggtctga
     5221 atctggatga gttgctatta taatatcaat agtttttatt cttttttgtc taagataact
     5281 aataattata ttttcgctat cttcatcacc tccatcaatt aatatattct tatttgtggg
     5341 tgtttgaact aaaatactat caccttgtcc aacatcaatc atatgtatag atagtagaga
     5401 ttttttgtca cagccgatta aaagtgatat tataataagt attgataaaa aaattttatt
     5461 tctcaataaa aaatcactcc attcattaca tgtttatata aatttatgat gggtaatata
     5521 agatatatat gacttattat attttatatt ttaaacaata taaatgaaat taataccata
     5581 ataaaattgg agttttttat atatacaaaa taaaattttt aaaattaagc aataaaaaag
     5641 gtaggaggat tttatgtttt tcaaagatat tgaactaaat tcaaaaaaag agttagaccc
     5701 atattttgat ttagtagatt atgaagcttg tgagtactgt tttagtactt tatatatgtg
     5761 gcaacatgtt tataagacag gatattatat aggtgaagat tttgccgttt tagttgggga
     5821 atacgaagga gatagcttct caatattacc acttgctaaa aaagataaac ttccagaagt
     5881 tgtagatttt gtccttgaat atttttctaa aaataataaa aaaatatact taagaggtat
     5941 tactactgaa gtagtagagt ttttaaaaga aaaatatcca ggtagatttg agtatataga
     6001 agaaagagat ttatttgact atatttatga tgcagaaagt ttaagaactc tagctggtaa
     6061 aaagaatcaa aagaagagaa accatataaa ctatttctta aaagagtatg caggtagata
     6121 tgaagctaaa ttattagata aagaaaactt tgatgagtgt ttagtactaa tgaaggaatg
     6181 ggaaagtaat aaggaagaaa ataatgaatt tgatgaaagt atggatgatg aattaatagg
     6241 tatcaaaaag atatttaacc attatgacat attaaaagat aaggtaaaag tgtttggtgt
     6301 ttatgtagat ggaaaattag aagcttttag tataggagag cttttaaatc caaatatggc
     6361 attgattcat atagaaaaag ctaatcccga tataagaggt ctgtatccat ttataaatca
     6421 acaatttttg gtaagtgaat ttaaggacgt tgagtttgta aatagagaag aagatttagg
     6481 tattgaagga ttaagaaaag ctaaactttc ttatcatcct tgcagatttg ttgaaaagta
     6541 cagtgttagg gaggcttaat agactatatg gagattaggt atgctaaaga agaagagata
     6601 gaaagtataa aagatatttg gagttattgt tttaacgata cggagagttt tatgaagtac
     6661 tattttaatg ataaatataa atctgaaaat actgtagtag ctttagatga aggaaaaatt
     6721 atttcttctc ttcagctaaa tcagtataag ttattactaa atagcaaggt atataataca
     6781 tcatatgtgg taggagtttc tacacttcca gagggaagag gagctggata tatgaacaaa
     6841 gtaatgaaat ttactttaaa cgaattgtat aaaaaagggc aattagtttc tatattgatg
     6901 cctattgact atagacttta tagaagattt ggatatgagc attgttacga ccagattgaa
     6961 tatactatta acacagatga tttaaaaaat tttaagtcaa gtggaaaaat gataaagtct
     7021 aatttatcac aaattgatga tttgatacaa atagatagaa cttttttaaa tgaggttaat
     7081 ggtaatgtgt taaaagatga acattattat gaaaacttat ttaaagagat tcaaagtgaa
     7141 gatggatttt tatacattca tgaaggaaat gaaaaagatg gatatatagt ttattttctt
     7201 caggaagata aaatgtttgt cagagagtta ttttataaaa atatagatgc ccttaaatca
     7261 atgcttaaat ttatatataa tcacaatact caatgtaaga tagttactat atctactcct
     7321 acaatagata agataaggtt tatattagac aaccctaaag atagtgatat aaaaataaaa
     7381 ccttttatga tgggaagagt tataaatgta aagaagttta ttgaagatat agatattgag
     7441 aaggatatta atagctcatt taatttatta atagaagaca agtttataga tgaaaataat
     7501 ggtctattta aaatttctat acagaataag aaggtatctg tagagcagtt agataaaaaa
     7561 ggtgcagaaa agcctcaaga agattttgat attaaattag atataaatac cttaacacag
     7621 ttatcatttt cttatataga tgtaaatgaa gctattttct taaatgatat aaaagatgtt
     7681 tcagaagaaa cactagaaac acttaattgt attttttcta aaaaaaataa ctatataaat
     7741 gaatatatat aggtagagta cgttaaaaga ttttaaaaat cttttgacgt atttttttgt
     7801 ttagataaca atttatcttg tttaaaaatt ttctatttga aaataatcta aataatatac
     7861 ttttatatgg gagtgattat aattcaaata agaagaccat tattaattat attaatgttt
     7921 gtggtagcta tatcatttat cattacaaat aacacaaaaa acgacgaaga acttaatgat
     7981 aagataataa ctatacaagg agttgtcaaa ggaaaaattg aaaagaaaag atacaatcag
     8041 tacaaggtag gggtttttct aataaatgat tatacaaaaa ataaaaattt gaagattggt
     8101 cagaaagtta atattacagg taaattcaaa agtttagata aaatgaagta tgatgatttt
     8161 gattatggaa ggtatataaa aagtactgga tataaaggta tagtgtatat aaataactat
     8221 aaaattattg ataaaaataa actttactct ttaatagggg aaataaagtt ttatattagt
     8281 aaaacttata ggtacttata taagaaaaac tcagatttta taaattctat actattagca
     8341 gaagttgaaa atttgactga ggaacaaaaa gaaattttta ccagaactgg aactagtcat
     8401 gtaatatcta tatcaggtct tcatacagga atattatgtg taattatatc ctttctatta
     8461 agaggtataa ataaattgta taaattatta atactgtcga tttttataac tttatattgt
     8521 ataatggtag gtgcgtctcc atccataatt aggtctatag catttgttat gattttttat
     8581 ttatcaatat ttattgatag aaagaaagat ggaatatcgg cattgtctct aattgggata
     8641 attttaatta tgaataaccc ctatgttata tataatgtaa gttttcaatt gtctttttta
     8701 gctacacttt ctatattaat attctataat aaaataaata gtattataaa attaagtatg
     8761 gtatcattga caatatcatc aaatatatta actttaccta taatttatta tacatttaaa
     8821 ggtataccat tgctttcaat tataggaaat ttaattatag tgccatttgt gggagtaata
     8881 atgtatttaa gtattgcaag tttaatcgta tttaaagtaa gtgtagtaat agctaaaatt
     8941 atatcttttt ttaatagtac atttatagaa agcatatttt ttttactaga aaaaatcagc
     9001 aatttaagtt ttgcatacat taatatagaa aatcctaaat tttatattgt tgttatttat
     9061 tacataggag tgttttttta catattttac atagaaggaa aagagataaa ggagcaagag
     9121 aatgaattac aaggatatta taaggaatgt aaaagagaaa aactttgaaa aaatgtatct
     9181 tttttatgga agagagtatt atttaattga gaatgcaata aaagcattta aagatagtct
     9241 aaacgaggga atgcttgatt ttaatttaga tataatagat gggaaggaaa tagtcttaaa
     9301 tcagcttata agctctatag aaacacttcc atttatggat gataggaaga tagtaataat
     9361 aaaagacttt gaactattaa aaggaaagaa aaagaatttt acagatagtg atgagaaata
     9421 cttaatagag catttagaca atattccaga caccacaact atagtttttg ttgtatatgg
     9481 agatgtagac aaaagaaaat cattagttaa gaaaatagga aacaatggaa ttgtttttga
     9541 ttgtgataag ctttctgata tggatttgtt taaatggata aaaaaatctt ttgctttaaa
     9601 tgatgttata atagataatt ctcaaatcat gtattttatt gaacaagaag gatacagaga
     9661 taagagtagt gaaaagacat tatctgattt agaaaatgaa ataaataaaa taagttcatt
     9721 tgttggtaaa gggaataatg taaccaatga tgtaattgat aaattatctc aaaaaaaagt
     9781 tgagaatgat atattcaaat taatcgatta tattggagaa caaaatgctt ctaatgcaat
     9841 gaaaatatta aatgatatga tacaagaagg cgaatctgta ttaggtatat tttctatgat
     9901 agcaagacaa tttaaaataa ttatgcaagt aagacagtta caacttgatg gatattcaac
     9961 aaaacttata gctgataaat taaaaatgca tcaatttgtt gtaggaaaag cattaaagca
    10021 aactaaaaat ttttctgatg atataattgt ggaaatatta aactatatat tagaaagtga
    10081 ttacaaaata aagacaggtc ttataagaga tactttagca gttgaaatgt tggttagtag
    10141 atactgtaaa agagaagcaa tttaaaaaat ctatatatta ataaaaaata aatagcaaga
    10201 tatgttatag ttcagagtag atgtaagcta aattaagttt gatatacaat caactataat
    10261 atgtctaact atttattttt tattgtataa atttacaaaa taaaaaaccc ctacataggg
    10321 gttattatat ataagaaata taaactggta attaagcgtt cataccgttt aatttttgag
    10381 ctaatttagc tacttttcta gctgcagcat tcttatgtat agttccttta gaagctactt
    10441 ggtatattct tttttgagcg tattggaact tagcaacagc atcctctctg tttccagctg
    10501 caacagcatc ttcgaatctt cttattgcag tttttatttg agattttcta actctattta
    10561 aagcagtttt cttttcgata acacttattc ttttcttagc tgattttata tttgccaccg
    10621 tgttcacctc cctgtatttt tttatcatat tcatcgtcaa catattagat tttaacagaa
    10681 ataaatctaa aaatcaagga ttattattta tatattttaa taatgttttt tattttgatt
    10741 ttttagactg taaattgtgt ataaataatt tgcaatatct tgtttttaaa ttggtatatt
    10801 ttg
//
LOCUS       DAELNY010000125        10779 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.125, whole genome shotgun sequence.
ACCESSION   DAELNY010000125 DAELNY010000000
VERSION     DAELNY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.125"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1188)
                     /locus_tag="KPE55_002668"
     CDS             complement(<1..1188)
                     /locus_tag="KPE55_002668"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG0493716.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDI"
     gene            complement(1211..1876)
                     /locus_tag="KPE55_002669"
     CDS             complement(1211..1876)
                     /locus_tag="KPE55_002669"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG0493717.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(1910..3022)
                     /gene="ribD"
                     /locus_tag="KPE55_002670"
     CDS             complement(1910..3022)
                     /gene="ribD"
                     /locus_tag="KPE55_002670"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG0493718.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3154..3275)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3464..5947
                     /gene="recQ"
                     /locus_tag="KPE55_002671"
     CDS             3464..5947
                     /gene="recQ"
                     /locus_tag="KPE55_002671"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG0493719.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6036..6147
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6289..7590
                     /gene="thiC"
                     /locus_tag="KPE55_002672"
     CDS             6289..7590
                     /gene="thiC"
                     /locus_tag="KPE55_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG0493720.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7644..7838
                     /gene="thiS"
                     /locus_tag="KPE55_002673"
     CDS             7644..7838
                     /gene="thiS"
                     /locus_tag="KPE55_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG0493721.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7831..8634
                     /gene="thiF"
                     /locus_tag="KPE55_002674"
     CDS             7831..8634
                     /gene="thiF"
                     /locus_tag="KPE55_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG0493722.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8697..9467
                     /locus_tag="KPE55_002675"
     CDS             8697..9467
                     /locus_tag="KPE55_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG0493723.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9484..10587
                     /gene="thiH"
                     /locus_tag="KPE55_002676"
     CDS             9484..10587
                     /gene="thiH"
                     /locus_tag="KPE55_002676"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG0493724.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10588..>10779
                     /locus_tag="KPE55_002677"
     CDS             10588..>10779
                     /locus_tag="KPE55_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0493725.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISD"
BASE COUNT         4082 a         1303 c         1833 g         3561 t
ORIGIN      
        1 aatatctaaa agatgtccca ttttatcttt tttagttttt aaatatagct tattaaagtc
       61 attaatttct tcattaatag ggattctatt ttcgattttt attccatatt tctctaattg
      121 atttatttta tctgggttat tacttaaaag atttatactt tttatgttta aattttttaa
      181 tatttgagca gccatataga aatctctcat atcttcttca aatccaagca ttaaatttgc
      241 ttcaacagta tcgtaacctt cttcttgaag tttataagcc ttaattttat ttaaaagacc
      301 aatacctcta ccttcttgct tcatataaat taaaactcca cttccatttt ccacaatttt
      361 cttcatagaa gattctagct gtaatccaca atcacatttt agagagtgga acacatctcc
      421 tgttaaacat tcagaatgaa gtctaactag agtattttct atatttatat ttccataagt
      481 aagggcaatg tgttcttcat ttgaataagt atctctatat cctatgattt caaaatttcc
      541 atattttgtc ggaaggaaag cactagatac tttttctata gtagtttcat ttattttgcg
      601 gtattctata aggtctttta tagtgatgat ttttaaatta tgcttttctt tgaaaatcat
      661 taaatcatca actcttgcca ttgtaccatc atctttcatt atttcacaga taagaccaat
      721 ctctttaaaa cctgcaagtc tagcaaggtc tactgaggct tctgtatgtc catccctgac
      781 aagaacccca ccagtttttg ctattaaagg aaagatgtga ccaggcgttt tgaaatcttt
      841 acttgtagaa ttttcatcac agaataattt tacagtttca gctctttcat atgcagatat
      901 accagttaga gtatctgctc catctatagt aacagtaaag gctgtttgaa atgtatctgt
      961 attttctcta accataagag gtatattaag acttttaaat tttttttcag tggcaggtag
     1021 acaaattaga cctctaccat atgtagccat aaaatttatt gattcaggag tagccatttc
     1081 agctgccatc aataaatctc cttcattctc tctactttca tcatcaacca cgattaccat
     1141 tttcccattt tttatatctt caatagcttc ttcaatagta ttaaacatgt aattcactcc
     1201 tttattattt ttaaaaacca tttttaaata aaaaatcttt atctatattt gatttatggt
     1261 tattactatt agaatttaat tccttgtagt tattagccat aaaattatta acatactttc
     1321 caattacatc gctttctaaa ttaacgaaat ctccaacatt ctttgtcaag agaatagtgt
     1381 taattttagt atgaggtata atagaaacct taaatatttc atcatctaaa tatgatatag
     1441 ttaaacttac accatcaata gcaacagaac ctttgagtat cattgaggat aaaagttttt
     1501 tatcaggtcg tatacttact aaaactgcat tttcatcttt ttcaaatttg cagatagttc
     1561 ctttaccatc aacatgacct gttactatat gaccaccaaa tcttgaagaa agactcatag
     1621 ctctttcaag atttaccttg tcatttgcat ttaataaacc taaatttgtg gaacgaattg
     1681 tctccatcat aacatcggct gtaaattcat tttttccgag gtttgaaaca gttagacaaa
     1741 ctccatttac ggctatacta tctccaagat ttgtgccatc taatatttta tttgatagta
     1801 tggttacttt tccactctta ccattagcag taatttttct aagtattcca acttcttcta
     1861 ctatgccagt aaacatccta acctcctata tgaatgtatt aacaaaaatt taatttaaaa
     1921 catctgattc tataagaata tcgccatcta tcaaagttac attattgatt gttaactgtg
     1981 ttgaatctga caaatgattt attccttctc cagaaacgaa tgtaggtgca tctttaccac
     2041 ctataatctt gggtgataga aataatttga ccttatcaac taacttattt ctaaagagac
     2101 tgtcattgag agtagcacca ccttcaacta gaacagaatc aatgtttaac tctcctaatt
     2161 tatacattac aaactccaaa tctactaagt tgttttttga aggagaaatt attgtttcaa
     2221 cattattttc ttttaacata gataatttta tactttcttt gcaacatgtg aaaactatag
     2281 ttttactggt tttatcttta accaaattac tattaagtgg caactttagg tgtgtatcta
     2341 ttactattct tgtaggatga gacacttttt cttgattgag cctacaagtt agtaaaggat
     2401 tatcgttcaa aactgtattt atgccaacca ttatactttg gtatttattt ctgtatttat
     2461 gagttagata ccttgatgat tcattagata tccattttga ttctagtgat tttgtagcaa
     2521 ttttaccatc tagacttact gctgatttta caatacaaag aggtcttttg ttttttatat
     2581 gataaaaaaa tgcctcattt aaatctcttg cttcatcttc taatattccc acagtaacat
     2641 caatattgtt atcttttaat ttttttactc cattaccaca aacaagtgga tttgggtcta
     2701 atgtagatat tacaactctc ttaatcttat tttgtataat tttatctaca caaggaggcg
     2761 ttttcccata atgagaacat ggttctaagt ttacataaag agtagagtct tttagacttt
     2821 gtttagcact gtttatagca tttacttcag catgattaga gccaaatttc tcatgatatc
     2881 ctttgcctat tatattacta gctttaacaa ttacacaccc aactaatgga tttggattta
     2941 caaagccttc tccattttta gccaattcaa tagctttttt catataataa atatcttttt
     3001 ctttttgatt cacaaaattc acaatatgta cacctcctgt ttaattggat aatttttaaa
     3061 caaaaaaccc tgagaattaa ctcagggttt agaataatct acaataaatg tttaataaat
     3121 gtagaacaaa attgctctac aaaaacactc attaaatatc ttcttccatc cagactttac
     3181 tgtcggcttt ggaatttaac caaatcaacc aagttttacc ttggctcgcg ggctataccg
     3241 ccgattggga atttcaccct accccgaaga ttaagtatta aatttatatt tagaatgtat
     3301 cataaattat tttactttgc aaactatttt tttaaaaaat atatgttaat atataatata
     3361 gaatgtctat aaactttgaa ataccacgat tgtgaatata aaaataaaaa tttgaacata
     3421 atcaggaaag aagtctaatt aatttaaatt aggagagtaa tatatgaata aaaaactttt
     3481 agaaatacta tcaaaatact atggatatac aagttttaga aaaggtcaag aatctataat
     3541 aaactctatc ttaagcgaaa aagatgtgtt agctattatg ccaacaggag ggggaaaatc
     3601 aatatgttat caaattccag ctttgatttt agatggaatg acaatagtaa tttctccact
     3661 tatatcactt atgaaagacc aagtagatgc cttaaagact atgggaatta gtgctgcttt
     3721 tataaatagt tcattaagta gcaaagaatt taatgaaata ttaaataata ttagaaaaaa
     3781 taattataaa atactctata ttgctccaga gagattagat gcacaagagt ttattgagct
     3841 aataaataat aacaatgtaa gtcaagttgc gatagatgaa gcacattgtg tatctcaatg
     3901 ggggcatgat tttaggttga gttatagaag gatttctgat tttataaaaa atttgcctaa
     3961 aagacctata gttacagcat ttactgcaac agcttcagaa gaggtaagaa ctgatataat
     4021 aaatttactg tgtttagaaa atccagatta ttatataact ggatttgata gagagaattt
     4081 atcaataaat atagtaaaat catctagtaa aaataagtat attttagatt atattcaaaa
     4141 tcataaaaat gagtctggaa ttatatatgt tgcaactaga aaggaagttg aaaatatata
     4201 taatggttta tctaagagaa atatatctgt gtcaaaatat catgcaggtt tatctaaaaa
     4261 tgaaagaagt aaaaatcaag aagattttat aaatgataat gtggatataa tggtagcaac
     4321 aaatgcattt ggaatgggta ttgataaacc taatataaga tgggttatac attacaatat
     4381 gcctcaaagt atagaaaact actatcaaga aataggtaga gctggtaggg atggagaaaa
     4441 aagtgagtgt gtacttttat tttctccagg agatgtacat atacaaaaat atttaattga
     4501 tattggagtt gaaaatcctg aaagaaaatt atttcagcat aagaagcttc aatacatgat
     4561 tgacttagtt tacagtaatt catgctatag aaagacaata ttgagttatt ttggagaaaa
     4621 ttatatagac aattgcaata attgtagcaa ttgtttagta gagggtgaaa tagtagataa
     4681 aactatagat gcacaaaaag tcatttcatg tgtagctaga atgaaaagaa gttatggtgt
     4741 tactactatt gtagatgtgc ttagaggttc taagaataaa aagattttac agcttggatt
     4801 aaatactttg tctacatata acattatgag agattattct agtgaagact taaagaattt
     4861 tgtaaataca ttggtttctc atggtttttt agatttagtt gagactctag gcaatggaaa
     4921 tagaggtagt tttcctacta taagattaaa tgagatgtct atgaaagttt taaaaggtga
     4981 agtgaaagta gagtttaaag aaattgttat gacaaaatca ttggaagtag aggatgagtt
     5041 atataatgct ttaagagaac ttagacattc aattgcaagt gaagaaagga tagctccata
     5101 tatggtattt ggagatggta cacttagagc catgtctagt agttatccaa ttaataaaga
     5161 agaaatgcta gatatctctg gagtaggaga aattaaatat caaaaatatg gtagagattt
     5221 tgaagcagta attaaagaat atgtcgaaaa aaataatata aataaaaaaa tagatgaaaa
     5281 aaatgtcgat tcaaagaacg ttaatagtga tttcttagag gttaatacag ataaagtttt
     5341 atatgaaaaa ctgttagtca ttagagaaga atatgcaaca aaggaaaaat tacctgcata
     5401 tatgatttta gccaataagg ctttgaaaga aataagtgga agatatccac ttgatgaaga
     5461 acaattaaaa gatatttctg gcataggtgg agtaaaattt gaaaaatatg gagctagtat
     5521 tttggcagag gttaataaat atgtaaaaga aaataatatt aatgttattt gggagaaaaa
     5581 gggcaaaagg aagttaatat tagatggaga aagtagaaaa aataatgaaa tagcattaga
     5641 tttgctaaat caaggaaaag atatacaaag tatcagtcaa gaaatagaag tttcactgtc
     5701 aaccattatt ggatatgtgc atgactatgt aaaggatgga catagtatat catttgaatt
     5761 aggactagaa aatttttata atgaagataa aaagaagatg attttagaag tttgcgaaaa
     5821 agttggctat gataatttaa ataacataaa aagaaaactt ccagattcaa taaagtatga
     5881 aaatataaga gctgtgatac ttgactctta tcttgaaaac ttaaaaaata atattatagc
     5941 agagtaattt ttaacttaat aaaaaataaa atttgtatta ttgtgttgaa ctaataattt
     6001 tgctgtgtta tactgaattt gtaaaaataa tgaaaaatta gagctagggg cgctagaatt
     6061 ggctagctga gagataaagc cgtaacttac aaaccctaat aacctgatca agataatgct
     6121 tgcgaaggga agctttttaa tataatttta ttatataaat aggcaaatta ttcaatgtta
     6181 ggagaaattt gtctaagtgt atatacgatt ataagagtgc tttccaaacg aggaaaagca
     6241 ctttttttat tttcaaaaac tataacttta atcttggagg attgtaatat gaactataca
     6301 acacaaatgg atgctgctag aaaaggcata ataactaaag aaatggaaat cgtttctcaa
     6361 aaagaacagg tagatgtaaa tgaattaaga gaattaatag caaatggaca agttgttata
     6421 ccagctaata aaaatcataa atctttaagt gcagaaggtg taggtaaaaa tttaagaaca
     6481 aaaataaatg taaatctagg aatttcaaga gattgtaagg atatagaaaa agaacttgaa
     6541 aaagttagag tagcaataga catgaaagct gaagctataa tggatttaag taattatggc
     6601 aaaactagag aatttagaga aaaggttgtt gaaatgtcac ctgcaatgat aggttcagta
     6661 cctatgtatg atgcagtagg atatttggaa aaagaattaa aagatataac agaagaagag
     6721 tttttaaatg taataagaca acatgctatt gatggagtag actttattac tatacatgca
     6781 ggtcttacaa gaagtgtttg ccaaaaaata aaaaatcatg agagattgac tcatatagta
     6841 tctcgtggag gttctttatt gtttgcatgg atggaattaa ataataagga aaatcctatt
     6901 tatactaatt ttgataaaat attggatata tgtgaagaat atgatgttac attgagttta
     6961 ggagatgctt gtagaccagg ttgtataaaa gattctactg atggtgttca aattcaagaa
     7021 cttgtagtat taggtgaact tactaaaaga gcatgggaaa gaaatgtaca agtaatgata
     7081 gaaggtcctg gacacatggc tattgatgaa atagaagcaa atgtagtatt agaaaagaga
     7141 ttatgccatg gagcaccttt ttatgttctt ggaccattgg ttacagatat agcaccaggt
     7201 tatgaccata taacaagtgc aataggagga gctttagcat gtgccaaagg agtagatttt
     7261 ttatgttatg taactccagc agaacattta agattgccta atttagatga tatgaaagag
     7321 ggtataatag ctgcaaagat agctgctcat gcaggagata tagctaaaaa tgttaaaggt
     7381 gctcgtgaat gggataataa gatgagtaaa gctagagctg atttagactg gtgtgagatg
     7441 tttagacttg caatagaccc tgaaaaagct aagagataca gagatgaatc aactcctact
     7501 catgaggata gttgtactat gtgtggaaaa atgtgctcaa tgagaacagt taagaaaata
     7561 ttaaacaatg aggaacttaa tctaatataa tttttggcta gtatgactgg aataaaaata
     7621 agctgaaaag aggaggtgat ttagtgaagg ttaatgggaa ggaaattgaa tttgaaaaag
     7681 atttgactgt tatagattta ttaaataaat ataatttaaa aagtgataga gttgtagttg
     7741 aagtgaattt agagataatt gaagaatcta actacaatac atatgtatta aaagacgagg
     7801 acattgtaga gcttattagc tttattggag gtggttaaaa taaaaatatt tgtaaatgaa
     7861 ttattaacag atgttgagga aaatacaact gcttataaag ttagagatag ctataacaat
     7921 aaatgtgatg tattggtgtt aaatggatat ccaattaaaa cagatatgac cctttgtgaa
     7981 aatgataaat taacacttat acaaaaagga gttaagccat cacttgatga attggaaagc
     8041 ttgttgattg ctagacatac tccaaatata cataataaat taaaaaaagg gaaagttgct
     8101 atattgggat tggggggact tggttccaat atagctatct ctttagctag aataggagtg
     8161 ggagaacttt tacttataga ttatgatgtt gtagaaccat ctaatttaaa tagacagcag
     8221 tattttatag atgatatagg aaaactaaaa actgatgcca tgatagaaaa tataaaaaaa
     8281 ataaatccat ttataaaatt aaataaacga gatatttttt taaacaagaa caatatggat
     8341 actattaagg attcagattt aataattgag gcctttgatg atgcttcatg taaagcacag
     8401 gtatgtaatt atgtattaat aaatttaaaa gataaatatt taatagcatc atctggtatg
     8461 gcaggttact atgattcaaa tataataact actaagaaga taaaagacag gttttatata
     8521 tgtggggatt ttgtgaatga agctaaattt ggtgaagggc ttatggctcc aagggttgcc
     8581 atctgcgcaa atcatatggc aaatttggca acgcaaatac ttatagatat gtaaatatta
     8641 aaattagata taaaaaagat tgaatattaa aaaaatttaa cgtggaggga aataatatgg
     8701 ataaattggt acttggtgga catgaattta atagcagatt attggttgga acaggtaaat
     8761 atggttcaaa taatatattg cctgaggtaa taaaagaaag tggaagtgaa ataataacaa
     8821 tggcacttag aagagtggat ttagacaata aacaagaaaa catattaacg tatattccaa
     8881 aagagatgac aatacttcca aatacatcag gagctactaa tgcagaagaa gctgtaagaa
     8941 tagctagaat atctagaaaa atgggatgtg gagattttat aaaaatagaa gtaatatcag
     9001 atactagata tttacttcct gataatgaag aaactataaa agctacaaag atacttgctg
     9061 atgaggggtt tatagttctt ccatatatga caccagacct ttatgctgga agaagactta
     9121 tagaagctaa tgcagcggca gttatgccac ttggagctcc aattggttca aatagaggtc
     9181 ttcaaatgaa agaaatgata agaataatga tagatgagtt ggatatacca ataatagtag
     9241 atgcaggtat tggaaaacca tctcaagcta tggaggctat ggaaatggga gctgatgcag
     9301 tacttgtcaa cactgctata gcttcagcag gagaccctgt acaaatggct agagcattta
     9361 aattagctgt agaaggtgga agagaagcat atattgcaaa aacaggaaat gtgtcagaat
     9421 ttgcaaatgc atcttctcct ttaacaggtt ttttaggcaa tttatagaaa gtggtgattg
     9481 gtgatgagtt tttatgatgt aatagaaaaa tatagggatt ttgattttga tggatattta
     9541 aataatgtta ctgataatga tgttttaaga agtttatcaa aagacaagct tgaagatttt
     9601 gatatattaa atttgctttc taaaactgca gtaaaacatt tagaagatat ggcacaaaaa
     9661 gcacataaat taagtgttca atactttggt aaaacagtat gtttatatac accaatgtat
     9721 atagcaaatt attgtgttaa tcaatgtgtt tattgtagct ataatataaa gagtggaata
     9781 aaaagaaaaa aattaactat ggatgaaatt agagaagagg gagaagtaat ttcaaaagaa
     9841 ggtttcaaac atctacttgt tttgactgga gaaagtagtt ttcactcaag tgtagaatat
     9901 ataggtgagg ctatagaaat attaagagag aaatttccct ctataggtat agaagtttat
     9961 ccaatggaag tagaagaata taagtacatt gtagataaag gtgttgaagg attaacagta
    10021 tatcaagaaa cttatgacga ggaaatatat aagagagtac atataaaagg cccaaaatca
    10081 aattataaat ttagactaga tgctcctgaa agaggggcaa aagcaggtat gaggacttta
    10141 tcaataggtg cacttttagg gctaaatgat tttagaaaag aaactttttt tacaatattg
    10201 catgggaaat acttaaaaac gaaatatcct catattgagc tttcatattc aacaccaaga
    10261 atgagacctt ttaaagggtg ttttgaggaa ttggtagata taagtgatac tgatttagtt
    10321 caagctatgg tatgtatgag attatttgac cctcatgcag ctataaatat atccactaga
    10381 gaaaatttag aaatgagaag tcatataata cctttaggag taactaaatt gagtgcaggt
    10441 gtatctactg atgtaggagg tcattctcaa gatgaacatg atactgctca atttaagata
    10501 aatgatgaaa gtactgttaa agatgtggag aaaatgctaa actcaattgg ttatcagcat
    10561 gtgtttaaag attgggaaag attttaaatg tatctgataa caaacagaaa attatgtagt
    10621 gaggaacgat atttagaagt tattaaagag tctatattga gtggtgtaga aaatataata
    10681 ataagagaaa aggatttaga atatcaagag ctaagaaaat tatatatgaa aataaaaact
    10741 aaaatcaatt gtatagattt tcaagagcaa atatcagat
//
LOCUS       DAELNY010000126        10629 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.126, whole genome shotgun sequence.
ACCESSION   DAELNY010000126 DAELNY010000000
VERSION     DAELNY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.126"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            270..728
                     /locus_tag="KPE55_002678"
     CDS             270..728
                     /locus_tag="KPE55_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0493726.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            789..1064
                     /locus_tag="KPE55_002679"
     CDS             789..1064
                     /locus_tag="KPE55_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493727.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1123..2397
                     /locus_tag="KPE55_002680"
     CDS             1123..2397
                     /locus_tag="KPE55_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0493728.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2527..3579
                     /locus_tag="KPE55_002681"
     CDS             2527..3579
                     /locus_tag="KPE55_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0493729.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3579..4610
                     /locus_tag="KPE55_002682"
     CDS             3579..4610
                     /locus_tag="KPE55_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG0493730.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4702..5514
                     /locus_tag="KPE55_002683"
     CDS             4702..5514
                     /locus_tag="KPE55_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0493731.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5517..6437
                     /locus_tag="KPE55_002684"
     CDS             5517..6437
                     /locus_tag="KPE55_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0493732.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6612..7058
                     /gene="rpiB"
                     /locus_tag="KPE55_002685"
     CDS             6612..7058
                     /gene="rpiB"
                     /locus_tag="KPE55_002685"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0493733.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7088..7753
                     /locus_tag="KPE55_002686"
     CDS             7088..7753
                     /locus_tag="KPE55_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0493734.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7863..9365
                     /locus_tag="KPE55_002687"
     CDS             7863..9365
                     /locus_tag="KPE55_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG0493735.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9677..>10629)
                     /locus_tag="KPE55_002688"
     CDS             complement(9677..>10629)
                     /locus_tag="KPE55_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0493736.1"
                     /translation="LIFFMYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNI
                     SKNIKNIENGNLKYKLKVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITN
                     VSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSS
                     GDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFEN
                     LICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKT
                     DGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         4235 a         1206 c         1965 g         3223 t
ORIGIN      
        1 aaaaaacaaa aaaatcattt ctctaacaaa aaaaaccaca aaaaatataa aaaagtatgg
       61 ctagttaagt gattaacaag taaaaattaa aataaaaatc aaaaaaaatt tatcaatgaa
      121 agtcagctaa aatcctggaa gagagcatta tttttgggaa aacattttct tttgtttcac
      181 aaaaaaagcc actttttggc acacaacatg ctttatataa agtcaagaaa aactaaggta
      241 taagacaaag gcaattagga ggtgtaaata tggagtttat aaattttgta agtgataaag
      301 ttatagacct taaaatggat tgtaaaacga aagaagattt ctttaaagaa atacataata
      361 aagtttttga tttaggcttt gtaaaagaag aatttggaga aaaaatatta gcaagagaaa
      421 atgtatttcc aacaggttta aatttaggtg attatggagt agcaatacca catacagatg
      481 cggaatatat taaagagcaa tttatatcag tctgtacttt taatgaacca gtagtattta
      541 gttctatgga agaccaagat gaaaaagtac ctgtaaattt ggctttcgta ttaggactta
      601 atcaaccaca cagtcaatta tctgtattaa cagaattaat gggaattatg caaaataaag
      661 agttagttga taagttgatg agttctactg ataaagaaga agttttaaag acactaaagt
      721 ctttataaaa ataaaaaagt actaaaaata aaaaatataa aaataaaaat tattaggagg
      781 aaataaaaat gaaaaaaata ttagtagcat gtggagcagg aatagcaaca tcaacagtag
      841 tttgtgacaa ggtagagaga ttagttaagg aaaataatat acaagcagaa gttatacaat
      901 gcaaaatagc tgaatgtgca acaaagcaag aaggagcaga tttaatagta tcaacaacta
      961 tactaccaac aacttataat ataccagcaa taaaagcaac agcatatata actggagtaa
     1021 atacagcagc attagataaa aaaatattag atgctttaaa ataatcctat catagataca
     1081 ttgaaataat attttataaa acagaaaaag gaggatttat aaatggataa attattagca
     1141 gtagtacaat acattttaaa tatgggaccg acagtaatac taccaataac aattgtagta
     1201 ctaggaatga tatttagaat taagtttaca aaagctttaa agtcaggtct tacaattggt
     1261 ataggatttg tgggtataaa cttagttgta aacctattaa catcaacact agggccagcg
     1321 gctcaaggaa tggttgaaag attcggactt aatttaacag taatagatgt tggttggcca
     1381 acaacagcat cagcaacatg ggcttcacca gttgcaccaa tattaatacc agtttgttta
     1441 ttagtaaact tactattatt atacttcaat aaaacaaaga tattaaacgt tgatatatgg
     1501 aattattggc actttatagt agcaggtgca gttggatata tagtaacagg aagttatgtt
     1561 tgggctatat tctgtgcaat actaatggag ttattagtac tgtttattgc agaaaagaca
     1621 gcaaaaaatg ttcaggagtt ttatgattta gaaggtatag cattaccaac tggttcaaca
     1681 gcttcatttg ctccacttgg atatttagta ggtaaatttg ttgaaaaaat accaggaata
     1741 aataaaatac aagcagaccc agactctata caaaaaagat ttggtatatt tggagaacca
     1801 atgatgatgg ggattatact aggtataata cttggagcac ttgcgggata tgatgtagca
     1861 ggaatattca atgtaggtat atcaatgggt gcagtaatgt tacttatgcc tagaatggtt
     1921 agaatattaa tggaaggttt aattccaatt tcagaatcaa taagagattt cttacaagca
     1981 aaatatggag acagagaact ttatataggt ctggatgcag cagtagcaac tggacatcca
     2041 gcagttatat caacagctct tatacttgtt cctataactt tgttcttagc agttatacta
     2101 ccaggaaaca aagttttacc atttggagac ttagcgacaa taccatttta tatggcattt
     2161 atagtgtgtt ttagaaaagg taatatagtt caatcagtta taacaggaac aatagtaata
     2221 gctatatcac tttatatggc tacaaacttt gcagatgttc atacacaatt attacaacaa
     2281 gcaaatatga tgccagaagg aacaacaatg gttacaagta tagatacagg tggaaacttg
     2341 ctaaaatggg gaatattgaa attatctcaa ttagtttctt catttggaat attctagtat
     2401 gtagttaata gagattactc ttttttagta aagtatgcga aaatttaaca tttagcagct
     2461 actaaaaaag agtaatattt tttataaatt tttaaaaata caaaatacaa gaggaggaga
     2521 tttatcgtga aagcagcagt attacatggc acaaatgata tgagatttga agatatagaa
     2581 ataaaaccat gtgaaagtga tgaagtaaaa ataaaagtta tggcagcagg aatatgtggg
     2641 tcagatcctc caagagtatt aaaacattgg aaatatccag ttccagctat accaggacat
     2701 gagttttctg gagtaattgc tgaagttggt aaggatgtaa aaaatgtaaa agttggagat
     2761 agagtagttg caattccttt tataccttgt aatgaatgtg aatattgtaa aagaggatta
     2821 ttttcattgt gtgatgacca tggaatgtta ggagctaaaa gttttggagc ttttgcagag
     2881 tatgtaaata taaaagctac aaatgtactt ccaataggag atatggactt tgaagatgct
     2941 gcaatgatag agccactagc agttgctatg catggagtac ttaatatagg tgttcaagtt
     3001 ggagacactg tagccgtaat gggtagtgga acaatggggc agttggtaat acaaggactt
     3061 aaaatagcag gggcaggaac aattatagca gttgatatat ctgataataa attaagagaa
     3121 tcaaaggaat taggggctga tataataatt aatgctaaag atattaaccc agtagaaaaa
     3181 ataaaagaac ttactggtgg aaaaggtgtt gacatagctc ttgaatgtgc aggttcaaaa
     3241 ataactcaag aacaatgttt acttataact aaaaagaaaa gtaagatagg atttttaggg
     3301 atagcatatt cagatataac tttatcagaa gaagcatttg aaaatatatt tagaaaagaa
     3361 ctagaattaa aaggtttttg gaactcttat tctgcaccat ttccaggaca agaatggact
     3421 aagggaatca atctagttaa tgaaggaaag ataaaattaa aagaaatggt atctcataga
     3481 ttctcacttg aagatactta caaagcattt gaaatgataa gagatagaaa agaagaattt
     3541 aacaaaatat taattttacc acaaggggtt gaaaaataat gaaagcagta gttaaaacta
     3601 aaccaggcta tgataatgta gaagttttag aagtagaaga accgaaagct acaggagaca
     3661 aggttaaaat aaaagtagaa tatagtggta tatgtggttc agatatacat tcatttaaag
     3721 gtgaatatgc aaatataaaa gccccagtaa cattaggtca tgaattttct ggaattgttg
     3781 tagaagtagg agaagatgtt aaaaatataa aagtaggaga tagagttaca tcagagacta
     3841 cttttgcaac ttgtgagaaa tgtatatatt gtgaaactaa agattataac ttatgcccaa
     3901 ctagaaaagg tattggtact caagtaaatg gaagttttgc tgagtatgta ttatcaagag
     3961 aagaaagcat acatgtactt cctgaaaatg tatctttatt atcagcagcc cttacagagc
     4021 cactagcttg ttgtgttcat gcagctcttg aaaagactac tatagaaagt tctgatacag
     4081 ttttaataat aggaccagga ccaattggtt tattattagc tcaagttgtt aaatctcaag
     4141 gtgctacagt tataatgtct ggtgttacaa aagataagga aagattagaa atagctaaaa
     4201 atcttggagt agatagaact gtaaatgtaa tggaagaaag tttggcagat gtagtgaatg
     4261 aaatgactga tggaaatgga gtaaacaaag gatttgattg ttctgggttt atgccagcag
     4321 ttaacgaagc cttaagactg cttagaaaaa aaggtacttt cgtacaagtt ggaatctttg
     4381 ctaaaaaatt aaatgaaatg gaccaagaag caataatcca aagagagtta caatatatag
     4441 gaagtagatc tcaaaagcca agttcttgga ttacagcatt agatttaatg gcaagtggga
     4501 aagtaaatac agaagcttta gttactagaa ctgttggttt agacgaattt aaagaaggta
     4561 ttacagcttt aatgaacggg gaagaaataa aagtagcagt aaaatcttaa aaaaacattt
     4621 gtattatttt aaaaaaaatt gtaaaatcta ctgtaggata aaaaaatgta aggtcttaaa
     4681 aaataacagg aggaagaaag tatgagcact cttagagatc attctaataa tattagaaaa
     4741 aacataataa aaatggttgc agaagctaaa agcggacatc caggtggttc attatcaata
     4801 gctgatatgt tgacagtatt atattttgat aagatgaacg tatgtgcaga aaatccaaaa
     4861 atggctgata gagatagatt tgttctatca aaaggtcatg cagcaccagc tttatatgct
     4921 acattagcag aaaaaggatt tttcccagaa gaagaattaa taacactaag aaaatttggt
     4981 tctaagttac aaggtcaccc agacatgaaa aaagtagcag gaatcgatat gtctactggt
     5041 tctcttggtc aagggctatc tgcagcaaat ggtatggcac ttgcaggaaa attagataac
     5101 aaagactata cagtatatac tatattaggt gatggagaaa tccaagaagg tcaaatatgg
     5161 gaagctgcaa tgacagcatc acattacaaa ttagataatt taattgcatt tgttgactta
     5221 aatggattac aaatagatgg ttctaatgaa gaagtaatga atgtaaatcc agtagatgac
     5281 aaattcaaat cttttggatg gaatgtaata gtaataaatg gacattcttt tgaagaaata
     5341 ggaaatgcag ttgatgaagc taaaaaagtt acaggaaaac caactgtaat aatagctaaa
     5401 acagtaaaag gtaaaggtgt ttcatttatg gaaaataatg ctgcatggca tggaacagct
     5461 ccaaatgcag aacaagcaga gcaagcattg aaagatttag aaggaggtct ataggaatgg
     5521 gaatagcaac tagagaagct tacggacaag tgttaaaaga acttgcagaa aataaagata
     5581 tagtagtatt agatgcagat ttaggaaaag ctacaaaaag tatatcattt aaagaagttg
     5641 ccccagatag attttttgac atggggatag cagaaggtga catgattggt acagcagcag
     5701 gtcttgctac ttgtggaaaa ataccttttg cgagtacttt tgcaatattt gcagcaggaa
     5761 gaggctatga gcaaataaga aactctgtag catatccaaa tttaaatgta aaaatagcag
     5821 ctactcatgc aggtgtaaca gtaggtgaag atggtggtag ccatcaagct atagaggata
     5881 tatctctaat gagaggtata ccaaatatgg ttgtattaaa tccagcagat gctttagaag
     5941 caagacaagc tattcttgca tctattgatt ataatggacc tgtttatata agattaggta
     6001 gagctgcaac tcctgatgta aacagtgaaa attataaatt tgaaatagga aaaggaactg
     6061 ttttaagaga aggttctgac ataacagtaa tagctacagg aataatggta gcaaaagcct
     6121 tagaagcagc agaagaactt gctaaagaag gtataaatgt agaagttgtt aatatatcta
     6181 caataaaacc attagatgaa gcactaataa aagaaactgc taagaaaact ggaaaagtag
     6241 ttacagcaga ggagcacagt ataataggtg gtttaggttc agctgtatgt gaagctttag
     6301 cagaaactaa agatgtagta gttagaagaa ttggtgttaa agatgtattt ggtcaatcag
     6361 gtacaccagc tgatttatta aaacactatg gtttaactac agaagatata gtaaaaaata
     6421 taaaagagtt gttatagata taaatatatc taaataacaa attgatttga cataagtcaa
     6481 ttaaagctgt cttaaaaata gaaagaaatt tctacaatgg gacagcttta tttttacaat
     6541 actttatttt tatgataatt ataaaatagt ctgaaagtaa tcaaaaaaat tataaaaaga
     6601 ggtgctattt aatgaaaata tctattggat gtgaccatgg tggttatgaa ttaaaggaat
     6661 atataaagga aaatttaata aataaaggta tagaagttga agatgtgggt acaaattcaa
     6721 aagaaagtgt agattttcca atatatgcaa aaaaggtaag tcaaaatgtt caaagtaaaa
     6781 aatctgattt aggcatactt tgttgtggaa caggaatagg tatgtctatt gcagcaaata
     6841 agtttaaagg aatcagagca gctgttgttt ctgactgttt ttcagctaag gcaactagag
     6901 aacacaataa tacaaatgta ctttgtttag gggaaagagt tattgggaaa ggattagcaa
     6961 tgagaattgt tgaagaatgg ttaaattcaa gttataatgg agaaagacat gcaagaagat
     7021 tacaaatgat tgatgatata gaaaaagaaa atttataaat atataattta taacaagggg
     7081 gagcgttatg tctattattt gtgcttcaat aatgtgtgca gaccaaatga agttaaaaga
     7141 ggaattagaa gctcttgaag aagcaggtgt aaaattactt cattgtgatg ttatggatgg
     7201 aatatttgtt aaaaacttgg ctatgggacc agagttatta aaatcaataa gtgaaaatac
     7261 aacaatacct ttagacatac acttagccac agaaactcca gataaatata ttgatatgat
     7321 gtcttatata aagccaaaat atatttcttt tcatgtagaa tcaagtacaa atgtaaaagc
     7381 tgatatacaa aagttaagaa attatggtat agggccagtg ttagcaataa gccctcaaac
     7441 aagtgtggat aaaatagaag aatatataag tttagttgag ggtatcttag taatgacagt
     7501 aaatccaggg tttgcaggtc aaaagtttaa tctttctgta ttggataagt tggataaact
     7561 tacagagata ctaaaagatt atgataatcc tccatttata gaggttgatg ggaatataaa
     7621 taaggatact attaaactta tgaatggtaa aaaagtagat atatatgttg ttggtacttc
     7681 agctttattt aatgataagc ctccaatttc atataaagat aaaatagaag aactaaaaga
     7741 aagtataaag taaccatgta ataaaattat aatatgtttc tgtttaatag taaaaattta
     7801 ggtggaaaca agaaatttta taatatttaa taaaaatttt ggttgagtaa ggagaatttt
     7861 ttatgggaga cctatataaa ctacaaaatg gtactgatat tagaggaata gcgtatgaaa
     7921 atgattctaa agaagttaac ctaactgtag aagaagtaaa aaaaatagct aaagcgtttc
     7981 atatttggtt aaaagaaaag actaagaaag ataaagtaac tgttgccata ggaactgatt
     8041 ctaggataac aggaagccaa tttagaagta ctgttattga aactttaact aatgacgatt
     8101 gtaatgtaat tgattgtgag attgccacaa ctccagctat gtttatgaca actattatgg
     8161 atggatacaa ttctgatggg gcgattatga ttacagcaag tcacttacct tattactata
     8221 atggtttaaa gtttttcaca gaaagtggag gtttagaaaa aactgatata aaagagatgt
     8281 tggatatagc tgttaaaaat gatagtgtag actatgagga agtaaataaa aaaggtgaag
     8341 ttatagtaaa aaatttaata gaagattatt ctaatttatt gatagataaa attagaaaag
     8401 gtgtaaattc aagtaaaaat tatgaaaagc ctttttctgg gcttaagata ttggttgatg
     8461 caggaaatgg ggctggtgga ttctttgctg aaaaagtttt acacatactg ggagcagata
     8521 cgacaggtag tcagtttttg aacccagatg gtatgttccc aaatcatata cctaatcccg
     8581 aaaacaaaga agcaatggaa agcatatgta aagctgtgtt ggataataag agcgatctag
     8641 gaataatctt tgatacagat gtagacagag ctgctattgt aggtaaaaat ggcaaaccaa
     8701 taaataaaaa tgctcttata gctgttatat catcaatagt attagaagaa catccaaaaa
     8761 cagctatagt aacagattca attacttctg aaggacttgc taaatttata aatgaattac
     8821 aaggaagaca tcatagattt aaaagaggat ataaaaatgt aataaatgaa gctataagat
     8881 tgaatagtga aggtgaagag tgccatttag ctatagaaac gtctggacat gctgctttaa
     8941 aagaaaatta tttcttagat gatggggcat atttaatagc taaaattttg ataaaagtag
     9001 caaagctatc cttggaagga aagactatag aagaattgat agaaaacctt gaagaagctc
     9061 aagaagagaa agaagttaga ataggcatta ataaagaaga ttttagacca tatgcagagg
     9121 atatattaaa agatttagat tctcatgtta agagtattga tggatggtct gtagcaccaa
     9181 ataattttga aggcataaga gttaattgtg atgaatcaaa tggagatgga tggtttttac
     9241 ttagaatatc actacatgaa ccattattag ctttaaatat tgaatcaaat aaaattggtg
     9301 gagcagaaca aatttattct aagttaaaac ttttcttaga aaaatatgat ttaaaaggtc
     9361 tttagggtaa atagaaagaa tatatttata ttgaagaata aatgtaaatt tgtacctaag
     9421 attacaaata ttacaaaatt gtaatcttag gtaacagaga tgtaactgat tatggaaaca
     9481 ctatgtgata ttatagatac agagagatga aatatatgat gaattataga gaaatataat
     9541 ttaaaatgta ttaatatcac accaaaaagg tataatcaaa aaatatgtaa atataaaccc
     9601 caacatttag aaaaagacct ctaaggaggt ttttttcttg tttaaataac ttaaaatata
     9661 tttttatatt aatatatcaa ttaactcttc taaaagtaag cattacctta aataaatcag
     9721 catctataat tatttccatg tgtgcatttt ctaattcaat tatattttga gcaattgata
     9781 aaccaagacc agagccatct gtttttctag atatatcacc ccttctaaat ctttctatta
     9841 actcttctgg ctttagattt agtttatcat ttgaaatatt tttaaacgtt aatattatat
     9901 tttcttcctt tataaacata tctatgtaga ccctagtgtt aggcatagag tattttagta
     9961 tattgcaaat tagattctca aaaactctcc aagttttttt accatctatc atggtaaaaa
    10021 cagcatattc aggtgtttct attataaaat ctaaactgct attttgaatt ttgtcttcaa
    10081 actctacaat actttgaatt aaaagttctc ttatatcggt ttttgttata ttaaattcta
    10141 catctccact acttagctta gatgcttcaa atatgtcatc tattaataat tttaatcttt
    10201 gtgatttttt atctaaaata tttatatagt catttatagt ctctctgtca aagtcattat
    10261 tttttaacat atcaacatag cttaatatag atgtaagtgg agtttttaag tcatgagata
    10321 cgtttgtgat aagctctgcc ttcatccttt cattttttat attagcatct acagcagttt
    10381 caagaccgcc tgttatatta tttatacttt tggctaaagt agatatttca tcatctccta
    10441 taacttttaa tttatacttt aaattgccat tttctatatt ttttatattt tttgatatgt
    10501 tatttatgta attaaactta acagataaaa agttgtatat ataaaaatga aaaactgtgt
    10561 ttacaagtgt aaataaaatt actatagcta ttgcctcagc ataatatata tacataaaga
    10621 atatcaaaa
//
LOCUS       DAELNY010000127        10601 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.128, whole genome shotgun sequence.
ACCESSION   DAELNY010000127 DAELNY010000000
VERSION     DAELNY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.128"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1625)
                     /locus_tag="KPE55_002689"
     CDS             complement(<1..1625)
                     /locus_tag="KPE55_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBG0493737.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTHQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFE"
     gene            1906..4083
                     /locus_tag="KPE55_002690"
     CDS             1906..4083
                     /locus_tag="KPE55_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0493738.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            4097..4519
                     /locus_tag="KPE55_002691"
     CDS             4097..4519
                     /locus_tag="KPE55_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0493739.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            4927..5730
                     /locus_tag="KPE55_002692"
     CDS             4927..5730
                     /locus_tag="KPE55_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0493740.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            5723..6391
                     /locus_tag="KPE55_002693"
     CDS             5723..6391
                     /locus_tag="KPE55_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0493741.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVSFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            6627..7289
                     /locus_tag="KPE55_002694"
     CDS             6627..7289
                     /locus_tag="KPE55_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0493742.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            7349..8146
                     /locus_tag="KPE55_002695"
     CDS             7349..8146
                     /locus_tag="KPE55_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0493743.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            8264..9454
                     /locus_tag="KPE55_002696"
     CDS             8264..9454
                     /locus_tag="KPE55_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493744.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            9563..10312
                     /locus_tag="KPE55_002697"
     CDS             9563..10312
                     /locus_tag="KPE55_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0493745.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         4124 a         1118 c         1802 g         3557 t
ORIGIN      
        1 aattcaaaaa aatcttttgt ttctctttct gataaatcta taaagtttcc atctttaagt
       61 ttataaaaag tacgtttttc ttgaaaagct ttcaaaatat ccttgtactc ttttggattt
      121 atatcagata tattaaaatt aaattctagg taattctggt tagtctgatt gattgaagct
      181 tgaattgatg aagcgttata tatttttttc aatttaaact tctcagaata atatatatct
      241 ccaaattcct ttagagattc taattgtaaa tctaaaaatg taaataggtc atggtcagtt
      301 cctttaaaca cataatgatt attttccttt gtaaattgtg aagaagtaag tttattttca
      361 atagtagctt ccttttgtat gttggggagc acaaattttt tatcttcatc atcatatata
      421 tactttacat cacaacatat gttgccatca atcatatcaa agtagaactc agatttaaaa
      481 tcttttgtta tattattttt tatgttatca tctaaatgaa cttcttgaga tatagatttt
      541 aatctaggaa ctagcaatcc taaaacatcc ttagaatcct ctttatcaaa ttttaactct
      601 ttagtctttg ataatatttg atatattttt gaataatcat gagctttttt atgtgataat
      661 aaatagatat tgccatcata taaaagtaca tcattttttt tagtaagtgg aacaggtagt
      721 gaatctttag aatataagac tattttatct tctatatttt taaaatcaaa ccttacaggt
      781 agagtatcat ttaatatctt tggatgatat tcctctttag aaaaaataag ggtaaaatcc
      841 ttgtacttaa tagcatttaa aaattgtttt aaagagcttg catttatctc cataaatttt
      901 tttgaatgtg atgaagaaaa gttgtatgaa agatttgtcc catattcttc tataatatct
      961 actaacttct catcttcatc agcaaaataa tgaatgttgg ggttgtaagt aaatttcttc
     1021 ccaaaatcta attcatatcc attaagtttt gaatatgcaa attcagtaat attttttaaa
     1081 atgtacattt tatcaattcc tattttaaat tcagcaattg ctttatttga atccacattt
     1141 atcaatacat ctaaattaac cctttctctt ttagtattgt ctaacaaaga aagtaattgc
     1201 ttgcttaaat ctaagctggt tttagaattt gtattagaaa gcttatcctt atctgcacct
     1261 aatatatcta ttagaatagg aggaacatct tttttatttt ttatttgttt taagcttttt
     1321 atcacaatag caactatatg cttacatata aaaatacttt tgccctttga ttgacagtca
     1381 gcgcaactac aatcattttc aattatctta aagtgcttta tatctatagt tattgaatta
     1441 aaataaaagc tataattatc ttcagaattg actccaccac gaaatgtcaa caggttattt
     1501 tgtaggtcat caatatctat gtctatataa tcaactaatt gatgagtata atattttaca
     1561 cctcttgacc atcttgatgg ttctgtattt cttcttatta gtttatatat catatctatt
     1621 ttcatattat tcaccttacc tcactgtata tattataatc taaatgcatt caatttacac
     1681 tctaaattta agactaaaat taaggcagat atgaatagct tttatctatc acaatatatg
     1741 tgtaaataac aatttactat tagattttta ttgaattatc ttataaacta tgaatgaaaa
     1801 ataattatat aaatagatta ttaataaata taaaatctta ttattaaaaa tattcttaga
     1861 aattacacta tataaaatat aaagtatgat atttgaataa aaagaatgta taataagtat
     1921 aataatttgt atttattatt gggaaaggaa aatgttaata tggatagtta tagacaactt
     1981 aaagctaaaa taccctgtaa ggaaacaggt atagaaataa agaggacttt atgtgatatt
     2041 tgttctccag gagtacattg tggaattgat gcatatgtta aagatggtaa agtaataaaa
     2101 atagaaggta caaaagaaca tccaatgaat aaagggttgt tatgtaccaa aggtctttgc
     2161 aatagagaat atatctatag agaagataga ataaaaacac ctctaaaaag aattggaaaa
     2221 agaggtgaag gaaaatttaa accaatatca tgggatgaag cttatagaga aatagcagaa
     2281 aaattaaata gagtaaaatc tgagtatgga gcaaagcatg tagctttttt tacgggttat
     2341 ggaaagtggt atagaccttt tttacaaagg tttgcatctt catttggttc acctaattat
     2401 ggtacagaat ctagttcatg ttcaacatca gcacagatgg ctggaaatgt tgctactgga
     2461 agaaacttta caatgcaaga tatggagaat gcaaaaactt atttagggtg gggatttaat
     2521 ccatactact ctaaattttt aaatagtgtg aatctaataa aattaaaaga aaaaggtcta
     2581 aaatttataa ttgtagaccc tagaataaca ccgacaactc aaaaactagc agatttacat
     2641 ttaagaatta aacctggaac tgatggagct ttagctcttg gaatggcgaa tctatttata
     2701 caaaagaatt taatagatag agaatacata gaaaagtatg tacatggatt taatgaatat
     2761 aaagaatatg taaaattatt tgatttagat actgtaagta gaattacagg agtttctaaa
     2821 gataatatag taaaagctgt tgagatgttg tcacaaggtc cattttgtat aaatgaaagt
     2881 gcagctccaa tagttcacca tcaaaatgga tttcaaaatt atagagcaat gatgtcttta
     2941 tctgctataa caggtaatta tgatagaata ggtggaaaca taccaaaaca agattacttt
     3001 gctggtaaga taggtggatt ccaaacacta gaaaaagagt ttgtaaaaga agttctacct
     3061 aaaaatataa atgaaagaat tggtggagaa agatttcctc tatggaatga aatggtgact
     3121 caatttcaag ctatggattt acctaggcaa atagaagaaa agacaccata tgaaataaga
     3181 gctttgtttt gtcatgggat aaatgctaga atgtttccta attcagggag actttttaaa
     3241 gcgttgaata gtgtagattt ttttgtgaat atagatttat ttatgacaga tacatcaaaa
     3301 tatgcagata tacttttacc tgcatgtaca tcatttgaga gagaagaatg taagagttat
     3361 ccaggagggt atattacata taccaaaaaa gttattgata aattatacga ttcaaaatca
     3421 gatgtagaaa tactttcaga tttagcaaat gctatgaata tagatgatga tttattaaaa
     3481 gcaggttatg aagcaagtat aagacatatg tttaaaaata catgtgtaga tgtagataaa
     3541 cttaaagaaa gtgatttacc attaaaatgt aaagatttta agccatatat agtaggttca
     3601 tatacaagag aagggtatga tactagcact tctaggtttg aattaaaatc tacgattatt
     3661 gaaaaatata aagattttgg actggatgca ctaccaacat atagaggatt taatgataat
     3721 atagatgatg aatatactca tttattgaca tcaggagcaa gaatttcaaa tgcactacat
     3781 tcaagacttc atgacatcaa actttcaaga atattaaaaa atgaagctag ttgtgatatt
     3841 tctagcttag atgcaaaaga attgggtgtt atcagtggag attatataaa aataactact
     3901 aagataggaa gtttggatgt aaaagtcaat gttactgata gagtcttata taagaatata
     3961 catatgtatc atggatatag agaagctgat ataaatagta ttatttgtga taattttgac
     4021 ccttatagtg gattccctgc gtatagaagt tgtaagtgta agattgaaaa aatgaataga
     4081 taaatggaga gatataatga aaagtttatt taaatttaat aaagaattat gtacagcatg
     4141 tggagcatgt ttgcttgcat gtatagacca aaatgataat aatatagaca atggtgaact
     4201 ttttagacat gtatttacaa tagaggaagg tgaaaatata gaatattttt cattagcatg
     4261 tatacattgt aatgagccaa aatgtttagg tgtatgtcct aaaaattgtt ttaaaaaaga
     4321 agaaggattc gttgtattag acaatcaaaa ctgtataggg tgtaaacttt gtgaaaaagc
     4381 ttgtgaatat ggagccttaa tttatggaaa tgatggtaag gcaaataagt gtaatggttg
     4441 tattgagaga tataagtgtg gtatggattc accttgtgta atggtatgtc ctacaggagc
     4501 attaaaattt aataaataaa ttaaatatag agaatgactt atacttaggt ataagatatt
     4561 tttacaaaaa gctgtattga gtttgttata atgaaaactc aatatagctt ttttatggat
     4621 atagttggat actataatgt gattcttagt aatagttttt tgctattgga caaagactgt
     4681 aaaacatatt aaacttatgt tgttcaaagt cgaagtataa tataataact agaaaattgg
     4741 tttatagtgc tataaaagca aatttagtaa ggtataaaat cagaggcaat cctttactat
     4801 aatatcaata tttgggagtt ctgttattgc agagctatta aacaattata atggtacaag
     4861 tgattcaacc tttgaaaatg tattatataa aaaattaaaa taagattaaa aaggagagaa
     4921 taaattatga aaaaacttaa attaattatt atgtttgcct tggtatcagc actgttagtt
     4981 acaggatgtt catcaggaaa taataaaaat aaagaagaac aaaaagttat aagagttgca
     5041 acatcaggaa catattaccc ttttgctttt aaagataaag ataaacttaa agggtttgaa
     5101 gtagattttt gggatgaatt tgctaaacga actaattgta aagttgaatg ggtattaact
     5161 gattttagtg gattatttgg attattagaa gctgataaag cagatgttgt atctgctcaa
     5221 cttactgcaa cacctgaaag agagaaaaaa tatgcttttt cagatgctta taattatagt
     5281 ggaacaaaca ttattgttag agaagatgat actagtataa aatcaataga tgatttagtt
     5341 ggcaagaaag taggtgtggg tactggagca gtagcaaatg aaatattaaa agaaaaatac
     5401 ccaaatgatg aaataaaaat agtaaattat tcaagtgcga ctcttaaagg gaattatcaa
     5461 gacctagaaa tgggaagatt agatgcagta gttgcgcagg atgttgaggc tttaatagct
     5521 ataaaagaac aaaaattaaa tcttaaaatg atagaacctc caattcaatt tggtgcctgt
     5581 tcttttgcgc ttcaaaaaaa tgaaaaaggt gaaaaatgga caaaagaaat taacgaagta
     5641 attgaagaga tgtataaaga tgggactttg actaaaatat ctgaaaactg gctaggaaaa
     5701 gacattacta aggagcctat taatgagtaa ttttaatttt gaattttgtc taagtatatt
     5761 tccatttttg attaaagcat tgggaataac aatactaata tctattgttt catttttttt
     5821 atcaatatta ttaggtgtaa tacttgcctt tttcacacag acaaaatcaa aagttttaca
     5881 atacataaca agagtgtatg tttcattctt tagaggaaca ccacttttaa ttcaaatatt
     5941 tttcttttat tttggatttc caatgatatt ttcttttatg aaagattgtt caccttattt
     6001 agcaatagta atttgtttag gtgtaaattc atctgcatat atggctgaaa ttatacgagc
     6061 atctattgag agtgttgata aaggacaaca ggaagcatgt tatgcttttg ggttaagtaa
     6121 atatcagtca atggttaaaa taattctacc tcaagcagct acatctgcag tacctcctat
     6181 tgtaagctgt acactggaca taattaaagg ttcttcttta ggtttaacta taggaataca
     6241 agatttaatg ggagtagctc aagtagaggc tgcttcaagt tttaaatatt ttgagatata
     6301 tatgataacg atattaattt actgggcagt atctataata ttgagtaata tacaaagcaa
     6361 agttgaaaaa agaataagaa gagcatatta gatttcaata tttaaaaatg ggatgatata
     6421 gatatattat tacttgtaaa taaattttta attttagaaa aataaactta gtaatctaaa
     6481 ttattgagaa aatatagtat caaaatggac tgtataatca agttaaaaaa tagtttttct
     6541 attgaaaaaa taaataaaat tctattgtaa tttaatatgc gataaagttt tataaaaaac
     6601 aacattaagc tagggggact tattttatgg cttttaatat gaaatatttt ttagaattat
     6661 ttccaacaat aatgaaatcg ttcaatgtaa ctgtgatttt ggcaataagt tcattagtat
     6721 tttcattaat tataggtaca atagtagcgt tatcatctta ttacaaagtg aaagtattga
     6781 atcaaatatg taaagtatat gtatctattt ttagaggtac acctcttatg ccacagttat
     6841 tctttttata ttttggacta gcatatatga gtgattttgt taaaaatatg gacccagtat
     6901 ttgcaacatc tatagtactt agtttaaata tgggagctta tatgtctgaa acgataaggg
     6961 catctatact ttcaattgat acaggtcaga tagaagcagc atatgcaatg ggattaacaa
     7021 atttacaaac aatgaaaagg atagtgatac cacaggccgt aagagttgca ctgccatcat
     7081 tattcaataa ttttatagat ttaattaaag ggtcttctgt agcttttgtt gttggagtaa
     7141 atgatataat gggtgctgcc aaatcacaag gagcgttgag ttttaagttt tttgaagtgt
     7201 atggagcagt tatggtaatt tactggtgta taataactcc tttaggttgg gggcaaaaaa
     7261 aattagaaaa gaaaataagc aattattaag ttattaagaa aatacataaa agtattttta
     7321 tataaaaaat ggtgtgagga gaagagatat gataaaaaga aatattataa aatgtttagt
     7381 ggcagtttta tcgttaacgt ttgtaattac tggttgttca aaaaaagagg aagtaaagaa
     7441 tgaaaaagtt ataactgtag cttcatctaa ttcagctgac ccatattctt atgttgataa
     7501 tggtgagcat aagggttttg aagttgacat gtggaaagag attggtaaaa gaagtggata
     7561 taagataaaa atggaaccaa caggttttag ttctatattt ggtatgttag attctggtaa
     7621 agttgatgtg gcagccaact tctttggaat gtctaaagaa agattagaaa aatacgatgc
     7681 ttctatacct tatggttctg attctgtgtg tatagctgta aaagatggaa ataacgaaat
     7741 taataagctt gaagatttaa agggaaaagt tgtagcagtt tcagaaggtt ctcaaggtca
     7801 acaggttgca actagtgtta ataaagataa tttgtttcaa gaaaaggttt acggtgatgg
     7861 aactagtggt cttcaagatt tagatttggg aagagttagt gcatggattg atgcagagct
     7921 tactattgtg ggagacgcta aaaaagctaa tatgaaagta agagtattag ataaaaaatt
     7981 atcagctact aatatagcat acttctttaa gaaaaacgat gataaatcta aaacgataaa
     8041 agaagatgta aacaaagcaa tagaagaaat gcttgcagat ggaacagtga aaaaaatatc
     8101 tgaaaaatgg tttggaatgg atgtaagtgc tgatatacaa aaataaatac tttaaaatta
     8161 aaatgtaaaa ctgtgataac tatagataag tatactaaat ttatctatag ttatctgtgt
     8221 attacaatgt atttagatta gtctttataa atcaaaatga gttatggagg tattgcaaat
     8281 gattgaaact aaaataaaat ccattgcact aaaaaataga gaaaaaatta taaaaataag
     8341 acgacagata cacagtaatc cagagttagc atttaaagaa tataaaacat ctaaacttat
     8401 aaaagaagag ctaaataaac ttaatataga atacataaat attgctggta caggagtatt
     8461 agctactata aaaggaaaaa ataatggtgg aaaaactata ttactcagag cggatatgga
     8521 tgctcttcca atcaaagagg aaaatgattt agaatttaaa tcaataaatg ataatatgca
     8581 tgcttgtggg catgatgcac atgtttcatg gttgttagga acagcaatga ttttaaatga
     8641 cataaaagaa gaattgaatg gaaatgtaaa attactattt caacctggcg aagaaaaagg
     8701 tggttctgat attgtaatta aagaaaatgt acttgaaggt gtggatgcat tagcgactgg
     8761 tcattgttgg ccaactattg agtcaggaaa aattggaatc gctagaaatt gtgctatggc
     8821 agcgactaat acttttgaaa taacaataat tggcaagggt gggcatggag cagagcctca
     8881 taattgtata gacccaattg cagttggaaa tgcagtatat tcatccattc aacagatagt
     8941 ttcaagaaaa attgacccag ttgtacctgt agtagtgtct gtttgttctt ttaattctgg
     9001 tgtttctaaa aatataatac cagatgtatg tacacttcaa gggactatta gagcgataag
     9061 caaggaacaa gtaattgaag taagtaaaat tctagaatat atagtaaaaa atgtatgtaa
     9121 agcacatgga gcagattgca aatttgaaaa gagtacagga ggagatgctg ttataaatga
     9181 caaaaacatg atagagatag gaagagaaag tgcttgcaaa atacttggag ctgaaaatgt
     9241 tgaaataatt gattttcctg caatgacagg tgaggatttt gctatttata tgaaagaaaa
     9301 acctggttta tttatgtata taggtgtagg agataaagaa aaaaatataa attacagatt
     9361 gcatagtaac aagtttaata tagatgaaaa atgtcttagt atagcacctt cattattttc
     9421 acaacttaca gtagattatt tatgtcaact atagagtaca ttcataaaaa tatatttagc
     9481 ctatttttaa atttaatgct agtatattaa ttttgatgta gaacatatta tttattatat
     9541 ttatttaaaa aggtggataa caatgataaa agtagaaaat ttacataaaa gttttaaagg
     9601 gacagaagtt cttaaaggta taaatttaga gataagtcaa agtgaagtag tagctatact
     9661 tggatcatct ggaacaggta aatctacgtt acttagatgt ttaaattatt tagttgagcc
     9721 tacaaaagga aagataatga ttggtgatgt aaaagtagat gtagaaaaag caacaaaaaa
     9781 agatataacc aatttaagaa aacatacatc tatggtgttt caaaattata acttatttaa
     9841 aaataaaaca gcattagaaa atgtaatgga ggcattgata gtagttcata aaaagagtaa
     9901 gaaagaagct agagaaatat ctttaaaatt aatagataaa gtaggtatgt tggataggaa
     9961 agatttttat ccatcaaagt tgtctggggg acagcaacaa aggataggta tagcgagagc
    10021 gatggcagta aatccaaatg taatattatt tgatgagcct acatctgcac ttgacccaga
    10081 acttgttgga gaagttttaa gcgttataaa atctcttgca aaagaaggta taacgatgct
    10141 tattgtaact cacgaaataa attttgcaaa agaagttgct gataaaatta tatttatgga
    10201 taatggtgta atagctgaag aaggaactcc tgatgaaata tttaataatc ctaaaaatga
    10261 aagaacagca aaatttttaa atatcattag gaaagaagaa gtatttagtt aaaagtgtat
    10321 aaattaagca ttagaagata tgtaagatac ccccattaaa tttataagtt agataataaa
    10381 tttaatggga gtatattttt attattagat ttattattta atttaaaatt agaaatgtaa
    10441 ctatttgaaa attcttttta aaagtatatc aaaatatcaa atacataaaa aaataatatt
    10501 tgacaaagta tcatatgtgt ttacaaaatc aactttatgg agttatatag tataggaatt
    10561 acaatgaata tactaaaatt ttatattaaa gtacaaagta t
//
LOCUS       DAELNY010000128        10567 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.129, whole genome shotgun sequence.
ACCESSION   DAELNY010000128 DAELNY010000000
VERSION     DAELNY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.129"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..890
                     /locus_tag="KPE55_002698"
     CDS             <1..890
                     /locus_tag="KPE55_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG0493746.1"
                     /translation="ENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYT
                     EFRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGI
                     IFSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIM
                     KRSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSN
                     STLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVF
                     FGVTIGAIWASVIIKRMIR"
     gene            994..2178
                     /locus_tag="KPE55_002699"
     CDS             994..2178
                     /locus_tag="KPE55_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0493747.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2385..3098
                     /gene="rpsB"
                     /locus_tag="KPE55_002700"
     CDS             2385..3098
                     /gene="rpsB"
                     /locus_tag="KPE55_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG0493748.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3177..4088
                     /locus_tag="KPE55_002701"
     CDS             3177..4088
                     /locus_tag="KPE55_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG0493749.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4173..4877
                     /locus_tag="KPE55_002702"
     CDS             4173..4877
                     /locus_tag="KPE55_002702"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG0493750.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            4903..5460
                     /gene="frr"
                     /locus_tag="KPE55_002703"
     CDS             4903..5460
                     /gene="frr"
                     /locus_tag="KPE55_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG0493751.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5463..5666
                     /locus_tag="KPE55_002704"
     CDS             5463..5666
                     /locus_tag="KPE55_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493752.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5695..6429
                     /locus_tag="KPE55_002705"
     CDS             5695..6429
                     /locus_tag="KPE55_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG0493753.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6442..7221
                     /locus_tag="KPE55_002706"
     CDS             6442..7221
                     /locus_tag="KPE55_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG0493754.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            8045..9856
                     /locus_tag="KPE55_002707"
     CDS             8045..9856
                     /locus_tag="KPE55_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0493755.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(9944..10525)
                     /locus_tag="KPE55_002708"
     CDS             complement(9944..10525)
                     /locus_tag="KPE55_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493756.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIAKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         4261 a         1086 c         1835 g         3385 t
ORIGIN      
        1 ttgaaaatga atttaatgcg gaagaaatat taacttacaa ttataaaata ctggattttt
       61 ctgaatccat atttaaacaa aaagcaaatt ttaaaaaata tatttttaaa aagaatatta
      121 tttttaacta tacagaattt agagataaag ttttatttaa tggttgtgta ttcttagaaa
      181 actgtgattt taatagaacc atatttagta aacactatat aaatgaccgt atatttgaaa
      241 aagtgaaatt taaaggacct gatttggtag ttaataaagt agaaaatttt ccacgaatgg
      301 atggaataat ttttagcatg tgtacaaaat ttgtgttaaa gaatgttgaa tatggaaaaa
      361 gtgagtatga gcatggcaag ataaattata gaatagctag aaatcaagca acaaaaattg
      421 gagaatatga gatgatagga ttttactatt ataaggaaag aatttatagt agtaaaataa
      481 tgaaacgttc taattatcct accttcagtg attatttagt tgaaaaattt tttgaccaaa
      541 tagcacgtta cacaacaggt tatggagaaa agccatggaa tatattatta gttattatag
      601 caataataag tgtttttgct ttattatact tatttgtagg catagaaagt tcaaatagca
      661 cacttgtagc tttagatata aataatatag gtgattactc actaagtgag attttcaaaa
      721 tgtatatgga tttatggtat tttagcatgg ctacatttag tacagtagga tatggagata
      781 tggttgctac aagtttgatt gggaaagcat tggcaggtat agaagtattt tttggagtta
      841 caataggagc aatttgggca tctgtgatta ttaaaagaat gataaggtaa tatttgtgta
      901 aatatggata ggtattaatt actagatata aattggacta aatagggaaa atttaaatat
      961 aaatatactt aataatccaa gaaggtgaaa aatttgaaaa atgcactaaa aaaaattata
     1021 tcattagcca tgattctagt ttttataata ccaaattatt catatgcaga tgataaatct
     1081 gtagacaagt attcaaaaac atctatactt atagaccaag aaacaggtag agttttatat
     1141 agtaaagaac ctgatatgaa ggtaccatta gctagtttaa gtaagatgat gactttttta
     1201 cttgcaatag aagctattga aaataaagaa gtaaaagaaa ctgataaggt aaaaatagac
     1261 aaatctattg catcagtaaa agggtcaagt tataaactta aagatggtga agaagtgcca
     1321 cttatagagc ttatgagagg tcttatgatt gtatcaggaa atgatgcggc aattgctata
     1381 gcaaaacatg tttgtaaaac gaaagaagca tttgtagata gaatgaataa aaaagcaaaa
     1441 gaaataggaa tgagtaatac acattttcta aatccaaatg gtcttccaat atacgattta
     1501 agcaacccca aaaaaccagc aaaagaaaat atctctactg caaaagatat agctattctt
     1561 ggaaaataca tgtttgacca ttatgaaaaa caagtaacag ctataactga tatggagaca
     1621 tatacaaatt cagatagaag ttttgaaaaa agcaatacaa atgcattatt aagaattata
     1681 ccagaagtag atggtataaa aactggatat actggcaatg ctggatattg cttatcattt
     1741 tctatggttg taaataaaaa tgataaaaat gagaaaaaac gtagagtaat aggggttgta
     1801 ttaggtacta accataaaaa taaaagaacc tctgcttcat tagctctact gaaatatgga
     1861 aaagaaaatt tcaatatgaa aaaagtaatt gataaagata gctttattgg taagaaatat
     1921 ataaaaggaa cgaaagagtt agaggttaca ttaaaagcaa aagatgaact ttatacaata
     1981 ttaaaagatg atgaaagtat aaagtcagaa gttaagatta aaaatataga gtaccctgtt
     2041 aaaaaaggtg atactatggg tattataaaa tattatacta attcaggtga cttacttgga
     2101 agtgtagata tagtaagtaa taatagtata gataacgtat cattaaaaac aaaattaaaa
     2161 atgaaatttg caaattaaat tcaaaaaatg gtataaaata cacaaaaaaa tataaaaata
     2221 gaaaatatgg aaaaaatatt gttatttctt cacctctgtg atatactact agaggtgaaa
     2281 aaataaaata aatcacacac agcattggga tccttaaaag gtgcctatta catggttttt
     2341 tagggagaag atcaagctgg aggtataaaa ccaggaggta agatatgtca gtaatatcaa
     2401 tgaaacaatt attagaagca ggtgttcact ttggtcacca aacaagaaga tggaacccta
     2461 aaatggctaa gtatatattc acagagagaa atggaatata tataatcgac ttacaaaaaa
     2521 cagttaaaaa agttgaagaa gcttacaagt tcactaaaga agtagctgaa acaggaaagc
     2581 caatcttatt cgtaggaact aaaaaacaag ctcaagatgc gataaaagat gaagctgaaa
     2641 gatgtggaat gtactttgtt aatgaaagat ggttaggtgg aatgttaaca aaccacaaaa
     2701 ctataaaaac aagaataaat aaattaagag aattagaaaa aatggaagaa gaaggcgtat
     2761 ttaatgttct tcctaaaaaa gaagttataa aattaagagc tgaaaaagaa aaactagaaa
     2821 aatacttagg tggaataaaa gacatgcctg aattaccagg tgcaatgttc gtagttgacc
     2881 caagaaaaga aaacatagct attcaagaag ctcatagatt aggtatacca gtagttggta
     2941 tagttgatac taactgtgac ccagaacaat tagactttgc tataccagga aatgatgacg
     3001 ctataagagc cgtaaaatta ataactggtg ctatggcaac agctgtaatt gaaggtagac
     3061 aaggtgctga ggaagaagta gctgaagacc aagaataata atttgacaat taaatggtaa
     3121 gggtcgccct taccatttat aatatggaat agaataagta ggaggaaact taaaaaatgg
     3181 ctaatataac tgctcaaatg gtaaaagagt taagagaaag tacaggcgca ggaatgatgg
     3241 actgcaagaa agctttacaa gaagctgaag gaaacatgga aaaagcagta gatttattaa
     3301 gagaaaaagg attatcaaaa gctgctaaaa aagctggtag agttgctgct gaaggtctag
     3361 ttgcaataga aatgaatgat gataatacag ttgcttctat ggtagaagtt aactcagaga
     3421 cagatttcgt tgctaaaaac gaagacttca aagtgttcgt taaagatgct gcttgtatgg
     3481 cattagctac tgataaagaa gatatagctt ctttattagg tgaaactcat aaagaaggaa
     3541 taactttaca agaagtttta aataatagag ttgcaaaaat aggtgagaaa ttagacttta
     3601 gaagatttgc taaagttgta actaatggac aagttgctgg atacattcac ggtggtggaa
     3661 aaataggtgt tcttgttgaa atggaaacag aagctagaga tgctaaagtt ttagaattag
     3721 gtaaagatgt agctatgcaa gttgctgcta tgaatcctaa gtatgtttca agagatgaag
     3781 tagatgctga atacatagca catgaaacag aagtattaac tcaacaagct ttaaatgaag
     3841 gaaaaccagc taatatagtt gaaaaaatgg ttaaaggaag attagaaaaa gaattaaaag
     3901 aggtttgttt attagagcaa actttcgtta aaaatccaga tataactgtt aaacaattag
     3961 ttgctgatgt tgctaaggca gtaggttctg atataaaagt tgttaaagtt gtgagattcg
     4021 aagttggtga aggtatacaa aagagagaag aaaactttgc agaagaagtt gctaagcaac
     4081 ttaagtaata accaaagaga gaacacgcag gtgttctctt ttttaaaaaa tagaaaaaat
     4141 tggatatgaa ggattgaaat aggaggtttc tgatggataa accaatgtac aaaagggtat
     4201 tgttaaaatt aagtggagaa gcattagcag gagaaagagg ttttggtata aataatgatg
     4261 tagttaatga tattgctatt gctataaaaa aaatacaaga aataggtgtt gaagtagctg
     4321 tagtagtagg tggaggaaac ttctggagag gcagaaccag tgaaggaatg gacagaacta
     4381 ctgctgacta tataggaatg ttagctacag ttatgaatgc aatggcgcta caggatgcac
     4441 tagaaaatat agatgttgct acaagagttc aaactgctat agacatgaga caaattgcag
     4501 aaccatatat aagaagaaga gcagttagac acttagaaaa ggaaagagtt gttatatttg
     4561 gtgcaggaac aggaaaccct tactttacaa cagatacaac tgctgcttta cgtgctgctg
     4621 agatggaagc agaagttata ttgttagcta aaaatgttga tgcagtatat gacaaggatc
     4681 caaaagtaca tgcagatgct aagaaattta cagaattaag ttacatggaa gttatacaga
     4741 aagaattaaa agtaatggat tctacagcta catctttatg tatggataat aaaattccaa
     4801 taaaggtatt tgagcttaca acagagaata taataagagc agtaaaaggc gaaaatatag
     4861 gaactactgt aaaataatga agaatgaagg aggagtaaaa ttatgaaact ggaaatacat
     4921 aagcaattag aagagaaaat gaatgggaca attgatgctt taaagtttga atttggaact
     4981 ataagagcag gaagagctaa tgcacaaatg ctagacaaaa taagagttga ttattatgga
     5041 actccaactc caataaatca aataggtgct atatctgtac cagaaccaag aatattaatg
     5101 atatctcctt gggataaatc tgctatgcat gaaatagaaa aagcgatagc taactcagac
     5161 ttaggattaa atccatcaaa tgatggtgag gttataagac tttctgtacc tgctttaaca
     5221 gaagaaagaa gaaaagaatt agctaaaaaa gctagtaaag ctgcagaaga atttaaagtt
     5281 agaataagaa atgaaagaag agacgccaat gagaaaatta aaaagatgga aaaaggcgga
     5341 gaattaactg aagatgaatt aaaaaaagca caagatgaag ttcaaaaaat gactgataaa
     5401 tttataaaag aaatagatac attattatct aaaaaagaaa aggacataat ggaggtataa
     5461 tattgaaaaa atattataac cttttaggtc ttcatgtaga tgaggtaaaa caatattttg
     5521 atgagcaaaa tataaattat accgtaaaat ctattgaagg taaaaaggat aaagaaaaac
     5581 ttgtagttcc tagagttata aaaatatctg agataggtga tagtgtagaa ctgataatca
     5641 catacttttc tgactcctta gtttaaggag tcagaacaat attggaggca aaatatgaat
     5701 aacaacatta tatatgatat agacttaaat aacataccta ctcatattgc tattataatg
     5761 gatggaaatg gaagatgggc aaaagctaga tttcttccaa gaactgcagg acataaggct
     5821 ggagtagaaa ctataagaga tatagtaaaa gaatgctcaa aattaggtgt taagcactta
     5881 acattatatg cattttcaac agaaaactgg aaaagaccga aacttgaagt tgacacactt
     5941 atgaacttat tatctacata cttaagaaat gaaatagctg aacttcacca aaacaatgta
     6001 aaagtaactg ctataggaga tataagtgct ttaccaaaaa cttgtataag agaattaaac
     6061 tcagctaagg aactgacaaa aaacaataca ggagttaact taaatttagc attaaactac
     6121 ggaagtagag cagatataaa aaatgctctt atagatatag taaaaaattg tgaaagtggt
     6181 aaaatagata taaacaatat tgatgaagat attataaaaa actatttaag cacaaagtct
     6241 ataccagatc cagacttggt aataagaaca agtggtgaac aaagattgag taacttttta
     6301 ctttgggaag ttgcttattc tgagttttac tttactgata tacattggcc tgattttaaa
     6361 aaagaagaac tacagaaggc tatatacgta tatcaaagaa gggatagaag atttggagga
     6421 cttaagtaag gagattattt tatgcttaca agaattattg cttcactagc tctggttcca
     6481 ttatttctat ttgttgtata tggaggtatt cctctatata tagctgagac agcaatagtt
     6541 tatattgcat tgcatgagtt ttataaggca tttaaaataa aagatgtaca tccaatattc
     6601 attataggat atttattttc tatttattta gctgtaaaaa atatttttaa tttaccatta
     6661 gaatatactt atgcagtaat ttttatttta tttttagcaa gtataattta tatgttgatg
     6721 ggcaaaaaca atgttataga tgtttcgatt acgtttttag gagtttttta tataggagta
     6781 tttttagatt ttataattat aactataaat ggatttgaaa aaggtagtat atatgtatgg
     6841 cttatatttg tgatatcttt tatgacagat atatttgcat attttagtgg ttatttactt
     6901 ggaaaacata agctaattcc taaagttagt cctaaaaaga ctatagaagg agctattgga
     6961 ggaataatag gaagtacact ttgttgtatt ttatttggat atttatttgg cattgatttg
     7021 cttcaattag cgataatagg aagtatagga agtgtaatag ctcaattagg agatttattt
     7081 gcatcttcta taaagagata tgtagggata aaggattatg gaaagataat acctggtcat
     7141 gggggtatat tagatagatt tgatagtgtt atattagttg caccttttgt atatagtgcg
     7201 attaaatttt ttattagata aagattggtt ggatttaaaa gagcaatcta taattgctag
     7261 taatgaaaat agctatgtaa cttttaaatg cagagatatt ctgcagttat tagttacatg
     7321 gctattttca ttgttcaaaa atttagaaga ccttggggaa ttgtataaga ataggtatta
     7381 ctttataata gtatactata tttgtagtga gtatattttt tatactataa ctatcattaa
     7441 aaaatatttt tttgtttaat aatttcgata tttttagcta tattatatat ttataatata
     7501 tatgtataaa tgtaactaat ttattatcca tttataaatt ttatttttaa atataattaa
     7561 taagaaataa gtaaattatt ggtcttatta cacaaaaata gatataattt aagtaatata
     7621 ccaaaaaaaa ggaatatatt tacataatat gataaaataa attcaaagag ttaaattgta
     7681 ggtaaatagt attttaatta tttattaaaa tatagattat gtatatgcca aatttaaaaa
     7741 gagacataaa gacattatac aaatatatta ttggggtgat attttataag aaaagaagtg
     7801 atgcagtaaa ttaattgagc aatttaaaaa tgatttaatg atagtgttga tgaatgttta
     7861 aaaagaaatt ttagaaaaac gtatattgct gtttgtgttg gaggtgatga atttgtataa
     7921 tttataggat ataaatgatt aaactattta agataaatac atatcactgt aaaatattga
     7981 taatcatttt ttataagaga ttgattattt gcaaattata tatattacaa atgaggtaac
     8041 aataatgaag aaaaaatatg attttttaat ttattctgct atttggctag ttattattat
     8101 tctttttata ttttctctat atcacagtat agagcatatt aaaattataa gtcacactgg
     8161 tactattcgc agtgaaactc aaaaggttgt taagcaagaa ttgaataatg aaagaaatga
     8221 tgacttgata aaacgactgg ataatattct gataaagcta agaacaggta atggagaaaa
     8281 tggatttcaa agatgtgata ataaagaatt tcaacaaaaa ctaaatcaaa tggattctat
     8341 gtgggaatct atgaagaaag aaattataaa agttagaaat ggtgcttcag gtgataaatt
     8401 atataaattg agtgaagagt attctgtgtt gtctaatcaa attgttttta tatctgaaaa
     8461 acactctaat gcaaaattgt attcttttgc tactgcacta tttatttatc ttatattctc
     8521 aacaataagt cttttaattt gggaatatta taataaaaaa aggtttaaaa gaatatttta
     8581 tactgataat ttaacaaaaa taaaaaatca ggttgcattt gaaaacagag cgattgaaat
     8641 tttgcacaat gcatctaaca aagagtatgt actgctaaat attgatattg acaattttaa
     8701 atatattaat gatacacatg gatatgaata tggagataaa gttctaataa tagttgcagc
     8761 tgcactttct aaaactttta atatcaaaga aacttgtgca agaattggtt cagataattt
     8821 tgtaatactt gcaaaatata gagattctct tttagaggat attcgggaaa tgctaactaa
     8881 tgctataatc tctgaattag atatgaatgt aactcaaact atatcttatt gtataggagc
     8941 gtacttagtt gaaattgata atttaggtta taaatccatc aattctataa tggataaggc
     9001 aaatattgca cacaaggtaa gtaaaacaag aggaatttcc tccacagtat ggtataatga
     9061 aaatctgtta aaacagttac aaatggaaaa tagtatttat aattatatgt ataaagcgat
     9121 agaaaatgaa gaatttcata tgtatttgca acctaaattt caaatatctt ctttaaatgt
     9181 tgtaagcgca gaagctcttg taagatggtt ttctccagaa cttggttttt tatcaccaga
     9241 tgaatttatt ccattgtttg aaaaaagtgg atttattatt gaattggact tttatatgtt
     9301 aaaaaaagcg tgttcatttg taagaaaaac gtttatgaaa aaaaatcaat atacatatcc
     9361 gattgcagtt aatttttcaa gggttactat ttaccaaaat agtttttatc aaaggttttt
     9421 agatactgtt aaagaatatg aaattccttt taagtatata gagatagagg taactgaaag
     9481 tgcttttaat gaaatatctc aaccagttat ttcaatttta gaggaactta aaaagttagg
     9541 atttttaatt tcaatggatg attttggttc aggatactct tcattaagtc ttttatgttc
     9601 tctatctatt aatggattga agttagataa aagcttactt aaagaaacct ttaaccggga
     9661 gaaagtatat agtattattc aatgtattat tgaaatgtca catcgtatag gcatgtctgt
     9721 tgtatgtgaa ggtattgaaa ctaaaaaaga cttggaattt ttaaatacag ttaaatgtga
     9781 tgtaggacaa gggttttatt tttctaaacc gatagaagaa aaagaatttt ttaacaaata
     9841 tgtaactaaa aaataatata attattttta tcagtctata cattgagtca ttttatatct
     9901 cgttaaatga ctcaatgtac tgttatgtat tttaaaatat aaattattta aaacgttttt
     9961 tatacttata tagaaattct tctatatttt ttgattgttc tcttatttca actgcttcta
    10021 tatctagtaa ataaagtata gaatcatttg taagttcaac atattcacca tcttctgaac
    10081 cgagtgcatg tctctcaatt tttaatcctt tttcttcata atattttttt gtttctatat
    10141 ataatgattc tcgcttttgt atgtagtctt tcacttcatt atttggaaag ttatatgaat
    10201 ccactaatgt ttcgccattt ataaaagttt tctcggtagc aactttttca tcccaacaat
    10261 ggatttcttc ccaagtaaaa aatatctcag ttccatctgg aatgaatgaa aacctggtaa
    10321 taatatataa ataattattg aaatttactt cataatattc aggaatatct tcaaattcca
    10381 tatagtaatt tgtataatta aaattactat tatctttttt tacaaagggt atttttgcaa
    10441 taaatttata tattgattgt tcatttttaa aaattcctat atcataactt ttttcaaatt
    10501 gattaatttt taataaatac aacattcttt ctccttttat tcaaaatata atttttataa
    10561 atttact
//
LOCUS       DAELNY010000129        10434 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.130, whole genome shotgun sequence.
ACCESSION   DAELNY010000129 DAELNY010000000
VERSION     DAELNY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.130"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            245..1477
                     /locus_tag="KPE55_002709"
     CDS             245..1477
                     /locus_tag="KPE55_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0493757.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            1491..2297
                     /locus_tag="KPE55_002710"
     CDS             1491..2297
                     /locus_tag="KPE55_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG0493758.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2409..3278)
                     /locus_tag="KPE55_002711"
     CDS             complement(2409..3278)
                     /locus_tag="KPE55_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0493759.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3650..5077
                     /locus_tag="KPE55_002712"
     CDS             3650..5077
                     /locus_tag="KPE55_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG0493760.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKMGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5154..5423)
                     /locus_tag="KPE55_002713"
     CDS             complement(5154..5423)
                     /locus_tag="KPE55_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493761.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5426..6868)
                     /gene="nhaC"
                     /locus_tag="KPE55_002714"
     CDS             complement(5426..6868)
                     /gene="nhaC"
                     /locus_tag="KPE55_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0493762.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6893..8059)
                     /locus_tag="KPE55_002715"
     CDS             complement(6893..8059)
                     /locus_tag="KPE55_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0493763.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIINPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8284..10404)
                     /locus_tag="KPE55_002716"
     CDS             complement(8284..10404)
                     /locus_tag="KPE55_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0493764.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
BASE COUNT         3743 a         1403 c         1539 g         3749 t
ORIGIN      
        1 aattaaagaa tatataacaa atttgaaata agtctaatat atgacttaat agattaaatt
       61 aaaataataa atttggacta tttatctatt tataacttaa attatatcaa attgataaca
      121 aactagatat tatacttttt tgtagaatag aattacaatt atataaagaa aaattctatt
      181 aatagtgaaa aaaaagtaga aaaaagtaga aattattctt ttataaagaa agggagggat
      241 aatattgcaa gagagagaag aattagtttt aaatccagtt ccaaaagagg aaagagttgg
      301 atggttagct ccattgttta atatgcttgg ttctaacata gctatatctg aactaatggt
      361 tgggggaact ttaattttag gaatgactct ttctaatatg attattacat caattatagg
      421 gaacttaata ttagttgcaa ttattatgat acaaggttat ataggttaca aagaagggtt
      481 aaatacgtat gtattagcta agggggcatt tggagagata ggaggaaagt atcttatatc
      541 tctattactt ggtataacta gttttggatg gtttggtgta caagcagggg ttgcaggact
      601 atcagtacaa aagatattcc caagtgtaaa cctaacatta gttacagtta tacttggatt
      661 atttatggtt gtttttgcac tgtatggctt taaagctatg gctaaattta attacttagt
      721 tataccacca cttatgatat taatggtgtg gggagttttt aaagcttttt ctacttatgg
      781 agttgaagct atttataatt atactcctca aacaactatg agtatggttg aaggtcttaa
      841 tattgtagta ggtcttgtta tagttggtgc aattatatca cctgaccagt taagatatac
      901 gagacgagta aaagacattt ggattatcag ttttttaggt ctagggatta tttcattatt
      961 ccaacaagtt gcagctggtg taatgtctat gggagcacct acttgggata taacagaagt
     1021 attggctaat ttaggttttg gatgggtagc gtttgttatt ttaatacttg catcttggtc
     1081 tacaaacgta tcaaatgctt attctggtgg attagcatta aaaactattt ttccaaatgt
     1141 aaaaagaaat gttttgactt tagttgcagg tctaataggt acaataatag cagctactgg
     1201 tataatattt aaatttcagt catttttatc tttcttggga atcgcagttc ctgcaatagc
     1261 aggaattatg tggtgtgagt attattttat acaaggaaga acttataagc atagagaagg
     1321 aataaattgg attgcagtaa taagttggtt aataggtttt gcagcgtcgt attatagttc
     1381 aaaaataaat ttcttaatac ctccgataaa tggaattgta gtgagtatgg ttatttatta
     1441 tatattaatg aattgttttg gaatcaaaga taaataaagg gggcaattac atgggtcgta
     1501 aggtgaaaat aggtataatt caacaacaca gtgtacttgg aaatgttaaa aaaaatatag
     1561 aaaaagcagt agagatgata gatgacttag gaaaacaagg ggcagatata atttgtttac
     1621 cagaactatt tgctacagga tataatttag agtctttagg tggagttaaa acattagaat
     1681 taataagaga acataataag tacatagagg aatcaatgtc agaagctgct aaaagaaata
     1741 atgtatacct gatatctcca tatggaacct tagaaaaagg gtctacacat gtttataatt
     1801 cagcagttat atttgataga aaaggtaaga ttatgggaga atactgtaaa aatcatctgt
     1861 ggtcattaga agcagtatac tttaaaggag gggaaaaagt agaggtatat gatgctgatt
     1921 ttggtagatt tggtgtaatg atatgttatg atgcaggttt tccagaagta tccagagaat
     1981 tgacattaaa aggttctgaa ataatattta taccatctgc atggagaata caagatgaag
     2041 atatgtggga tttaaatgta tctcaaagag ctttagagaa cactgtatat acagtaggtg
     2101 taaatttagt aagtaatgac tctaatttaa tactatttgg aaagagtaaa atatgcaatc
     2161 ctagaggtac tgtaataact caattagata catacaggga aaaatatgta ttatcagaga
     2221 ttgatttaga tgaaataaaa aagtataggg aagatatccc ctatttgaaa gacagaaaaa
     2281 gtagctataa catataattc tatactataa tacaagttca atataaaatg ataaaaagag
     2341 gatgtatcaa tattaagatt aaatttatta tactttgata tatcctcttt aatattatca
     2401 actttgattt aaatattttt aaaatagttg ataagaaagt ttttgaacaa caacacagct
     2461 ttagattcat agttttcgct aaaccatgaa agatttaaat atcttttaaa ttcaatatct
     2521 ttgatatgta acaacttaat ctcagggtct tgagataatc cccatgattt tccacatata
     2581 aatccaactc cttgaagtga ttttataaga gcataaattg tatatggtga atcagactca
     2641 aatgctattt tgggtttgaa attagctgat tcgcacagga tatcaattac ctctctataa
     2701 ttctcaccct tcgttataac tataaagttt tcatctgaaa cttcgcttag atatacttca
     2761 tcctttaatg ataaaggatg gtttattgaa actcctaata gtatttcttc gcatgttaaa
     2821 gttatgctat tttctgagtt tagctttgta aatgaagaag atatgtacaa atcaaaatca
     2881 ctttttttat aagatgatgg caaagtatgt gacacattaa aagtaatatt gggatatagt
     2941 tttctgaaat tactcaaaag tgatggtaac acattagccg ctgcaggaga taatatcttt
     3001 acttctcctg aattttctaa agaagtgtct tgtaactcct tttttgaact ttctactaaa
     3061 tctaaaatgc tatctactct ttttagaaac aatcttccat actcatttaa aacaatatat
     3121 cttccttttc tgtcaaataa ctgaactcca agttcttttt ctaaggaaga tatagttttg
     3181 cttaaagctg gctgtgctat atttaacttg tgtgatgctt ttgttatgtg ctccattcta
     3241 gcaacagttt gaaaatactt aagatgcaat aaatccatag tataaaatac acctctttta
     3301 atatgacttg agttatatca attttataat caactccaca ttatacatta taatataaag
     3361 tttatataat aaactacaat atattaaaaa gttatttaaa ttgttattta tattttgtac
     3421 ataatctaat taaaaaaagt aactttaaat gattaactaa ggaaatattg ttacaaactt
     3481 tagttatata aaaaattcat ttgatgagca aagtattaat gtcaagtaag aataaaagct
     3541 tattagataa gaaaaataat attttataat aatgatgtta cttaaattat tatgttatac
     3601 tattttaaat aggaaacatt tagatactat aattttaggg ggagtcaaga tgaaattaac
     3661 taaatcaata tcatcaatgc tgtgcttatc tctagtttta agcttatcac cagtagcata
     3721 tgcaaatgaa ataagtagaa caacagttga gaatacttta gtaggtcagg atagatatca
     3781 aactgctgtt gaagtaagta aaaaaggatg gagtagtgca aatgaggctg tagttgtaaa
     3841 tagtgaatct attgtagatg cattagctgt aacaccattt gctaaattaa aaaatgctcc
     3901 tatattattg acagaaaaaa ataatctaaa ttcacaaact gagaaagaat taaaaagatt
     3961 aggagttaaa aaagtttata taattggtct atctagttct gtatcaaatg atgtgcaaaa
     4021 acagttagaa tctaataatt taactactga tagagttggt ggtagtgata gataccaaac
     4081 agctttgagt atagctaaaa aaatagaagg attaaaagat atatcagaaa tagctgtagt
     4141 aaatgggtac acaggtcttg cagatgcagt tagtatagct tctgtagctg ctactaatgg
     4201 aatggttata cttccagtat ctgatgcatc tggtatatct agctttaaag attttataga
     4261 atctaagaat ataaataaat catacataat aggctctact aatgctgtat cagataaaat
     4321 aaaacaaagt ttacctaatt cagaaagaat aggtggagca gatagaaatg aaactaatgg
     4381 taaagtaata gaaaagtttt atacatctaa taaattaaac aatgtatttg tggctaagga
     4441 tggaatgaaa aatcaaaatc aacttataga cgctctagca gtaggagtac tagcatcaaa
     4501 agaaaattct cctgtactaa tcgtaggaga acaattaagt gcaacacaaa agagtgtatt
     4561 ttcgaataaa aatgctacct ctataactca agttggtagt ggaggaaatg aaaatgcatt
     4621 taaagaatta aaagatttac aggcaaatag taacaataag agagtaatgt atgtaactaa
     4681 tacagataag gtaaatataa gaaatgatgc tacaatagaa gcttctgtta taggatatct
     4741 aaataatggt gatgaagtag aagttttaga tgtgttaaaa actggatggg taaaaataaa
     4801 atacaatgaa ggtattggat atgttagtgg gtcttatttg acaaataata agcctgataa
     4861 tagtaatgaa aatataaaaa taaagtatgt aaaagaaaaa gatggtctaa atgttaggaa
     4921 aggaccaagt acagaagatg aaaaaatggg tcaactttca tatggaagca aagtagaaac
     4981 agtagaaatg tttgctactg gatgggtaaa aataaagtat aatggtggat atggttatgt
     5041 aagtaatgat tacttatctg atacaccagt tatttaatta taagtaacaa taaaaaaact
     5101 gctttaattt gtaaagcagt ttttttataa aaatttaagt ctaataacta tttctatttt
     5161 gataccttaa tcctgggatt tattattatg gatattatca acatacaaac actccaaatc
     5221 cagtgtctaa ttccaaactt agatattaat aaattcagtc ctgctatggt aaataagcag
     5281 tatttaatta caattaaaag tgtatttata gaaagcccct tcatagaaag tatcctaaat
     5341 ccaataatag acattctaag tcctataaat gccaataata tacctgcaat atcctgaatt
     5401 aagtaatagt attcaaattt catattcaat caactcttct ctgagcagta tcaaatattt
     5461 ctaactcttc aatttcatca tctgtgtatg taagcatagt aaaaccagtt acaccataaa
     5521 taaatgaaat tataggtgta atccaactta agaaacaata tggaataaat tctagtgggc
     5581 ttacaccaag agtctgagca cagaaaacag catttgaatt ccatggtatt aatggaccac
     5641 caagtgtagc tgtatcttct ataattctag atagattttg tggttttaat ttaaattctt
     5701 tgtatattgg agacattaga gttccgacaa atacagaagt gaacatcatt gatgaactaa
     5761 atgcatttgc aaagtagctt actataaaag tagacccaac caatttagtt cttgattttc
     5821 ctattttagt tataagaggt tgaagcagta cttctaagaa tccagttttt tggagaatac
     5881 cagcaataac gaaaactaca aatactagta aaacggtttc agccatactc attattccac
     5941 cacgatttaa tagtttgtcg gcatagacaa atccagtatc tatagtaaat cctgatagca
     6001 tgtaatttaa aacatcacct attttttctc cttcttgaaa tactgctata ataagtccca
     6061 tgatagctga acttagtata gatataatgg gtggcttttg tagcaataaa agtaataata
     6121 aaaatattat tggaatcatt gctactaaac caatatgaaa gtttgaattt aatacatcct
     6181 tgatttgatt tatttgaaca tagtctatag tattatttga gtacttaaat ccaattactg
     6241 tatacagaac tatacatatt acgtatgcag gtcctgtggt ataaaacata tgcttcatat
     6301 gtgtaattac atctgtctta caaactgctg cagctaagtt tgtagaatct gatagaggtg
     6361 acatcttatc tccaaagaaa gcaccagaaa tgactgcacc agctgttaaa ccaagaggaa
     6421 tgcccatact agtaccaata ctcatcattg ctataccaga agttccaaga gtcccccaag
     6481 atgtaccagt agcaacagaa gttaatgaac atacgattaa tgttgtaagc agaaaatatt
     6541 taggggttat tattgttagt ccactatata ctactgttgg tattgtacca gcagctatcc
     6601 aagctgctat tagtacacca acagaaagta taattaagtt tgattgaaag gcatctttac
     6661 caacttcaaa tccaaaagct tctatttcat cattagtata accaagtttc atggctgcag
     6721 gaactactat aagccatgaa agtaaaaaca ttgtagttat agaagcatta aatacaacta
     6781 taccaactga tgttagggct attattgcaa gcattactaa caaggaatat ctaaatgaag
     6841 gtatttttct ttctgtattc attttcatag tgcaaattct ccttaaatat ttttagttaa
     6901 ttgcattttt tattcttata agagcttctt caagcatact tcttggacaa gctaagttta
     6961 ttctttgata cccacttcct ccaatgccaa agctgtttcc ttgatttaga gcaactttac
     7021 ctttttgtac aagtatactt tctagttcct catcacttaa tccaagtgca ctaaagtcta
     7081 cccataataa ataagtacct tctggttttc taactttaag ttttggcata ttttcattta
     7141 tatattttat agcaaaatct atattagatt ctaaatattc taaaaatgat tctagccaag
     7201 attcaccatt attatatgaa gcttcagttg caactaagct aaagcaatta tttctcttta
     7261 tatcaattct ggtaaaggca tcatctaaca atttatagtc tttttcatca ggtagtatta
     7321 catatgatga ttgaagacca gctatattaa aagttttagt tggagccata caagtaattg
     7381 tatttttttc aaattcttta gaaatagatg ccataggaat atgcttatgt ttttttaata
     7441 taatgtcact atgtatttca tcagatatga tttttacatt atgcttaaga caaatatctc
     7501 ctagtttttt taattcatct tttgtccata ctctaccaac tggattatga ggattacaaa
     7561 gtatgaataa ttttacatct ttaatcttgt tttcaatgtc ttcataatcc attatgtagt
     7621 ttccattttc tagtttttgt aatgggttta taattagttc tctattgtta ttttttacaa
     7681 cactattaaa agggctgtaa actggttctt gtatcattat tttatcattt gcttttgtga
     7741 gttcatttat taaaagactt atagctggga taacacctgg actataaatt aaccactcac
     7801 tttttatttt ccagttatgt ctcctatata gccagtttac tatagattca ttgtaagaat
     7861 ctggtcttgt tgtgtagcca taaatctctt gttctagtct attttttaag gaatcaatta
     7921 tacaaggtgc agccttaaaa tccatgtcag ccacccacat aggaagtaaa tcattagttc
     7981 catacttttt ttccatttct gaccatttag atgaaaaatt attgcttcta tcaacaatct
     8041 cattaaaatt ataattcatt gtaaatacct ccagtaattt taattttatg ttaataatat
     8101 taagcaaaca acgtgccaat attgataata aaaaatattt attcacaagt taatatttaa
     8161 tattaagtaa tttcagagca tagataagat aaaaatatta attatactta aaactttgtg
     8221 gaaaaggtta tttctaataa atttttaaat atatgaaaat gtaattaaat aggttaaata
     8281 ggtttaactg tttaacttgt tagacctatt tatatttttt agaaattcta ctccttttga
     8341 tgttataatt gaacctccac gaccaacaag tatgtctaca agcccaaagt cttgtagttc
     8401 taaaagcata tttctaatct gttgttctgt caaaaatcta ttttcctcta aagctatttt
     8461 acataagctt tttctaccta ttctttcttt taaatcataa gcctttttta aattatccaa
     8521 tataaatatg tattcatcta gtgacctttt aaaattatag tcgtatttca tgatgttttt
     8581 gtctttgcca atgttgaatt gatacttttt tatattatca cttactttat tacaaacggt
     8641 tctactataa tttgaatcta tagtatccaa tatatattca ggtaaatcac atatttctat
     8701 aatatctttt tctaagtaac agaagtattc tcctaagttt cttaattctc ttacatttcc
     8761 ttcccatcta tacattttaa agatttcttt tacttcctca cttagaataa aattacatgg
     8821 aatatcatat tttaatgaac caaaaatttc aaatatatca gcaggtcttt ccctaagtgg
     8881 atgtatttta aggggaagaa cattcaacct aaaatataaa tctcttctaa acatattttt
     8941 agatacaagt tcttttaaat ttctattagt agctgcaatt attcttacat caatgtcaat
     9001 gacattatta gaaccaatac gtcgtacttg tttttcttgt atgactctaa gtaatttaga
     9061 ttgagaattt aaatccattt ctccaatttc gtctaaaaat atagttccat tgttggctaa
     9121 ttcaaataaa ccaattttcc cacctttttt agcaccagta aaagcccctt catcatagcc
     9181 aaataattca ctttgcagta gattttcttg aaaagtacta caatttactg cgacaaaagg
     9241 accatctttt ctcctagaag cactatgaat ggattgagca aataattctt taccagtacc
     9301 actttcacca attataagta tagatgaatt tgattgagcc attttattag caattttttt
     9361 tgtattaata gtttgtatac tagaacctaa tatatcatca aatgtgtatt tactgatatt
     9421 accagagttt actagttgtg ctcttaattt agcttgcttt ttttctaatt gagaaaactt
     9481 tgtcatcttt agtataaatc catcaaatac atttaattgt atatgtttaa tctctaaatt
     9541 tatatcaata tgatttattt taatcaattt ctcaaaaaat ggagttttat tggtcaatat
     9601 gctttgaaaa ttgatgtctt ttatgcaatc accaacaaat gagtcaatca tttcatttgc
     9661 atttatagac aaaatttttc tagccatatg attatagaat tgaattattc catcgttatt
     9721 tgtgcatata attccatcat caattataga tagtaacaaa tcaaattgtc tttcaaactt
     9781 atttgcatct aacaataact cacctgtact ataacttgta ggaattattt tttctacgta
     9841 tttttttatt aggtcatctt ttattagatg ttttaatttg gtttttgtag caatttcaac
     9901 aatacaactt aaatctatta ttctgtattt taaatctata acttcacaat ttttaaactt
     9961 aggcaagatt ttttcgccag gagttaggat tacagcatct gtgggagtaa actcaatctc
    10021 tggatagcag ggcaggatat ttatattgtc tattcctaat ctatgtatta aagaaattgt
    10081 ttctattgcc atatctttac ttaagttata tacataaata attttatctt tttctaattt
    10141 ggatatcata tcaatactgt tcttttcaaa tgtcaacttg ggaacaatga tttgagcatc
    10201 atagtgagat aattttacta tttcatcata ttttaaataa gcagatagga gtataagttt
    10261 ttctttaaaa aactttaaat ttccctcttc aaatgagtat aaatttatct ttagattatc
    10321 acctaagaat tgtcttattt gattttcata aatttctcca gcgtcttttt ttaaacttac
    10381 aatagctaat tccatttgtt tcattttaac ctctcataca aatttgactt attt
//
LOCUS       DAELNY010000130        10387 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.131, whole genome shotgun sequence.
ACCESSION   DAELNY010000130 DAELNY010000000
VERSION     DAELNY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.131"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..865)
                     /locus_tag="KPE55_002717"
     CDS             complement(<1..865)
                     /locus_tag="KPE55_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0493765.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIH"
     gene            1282..2685
                     /locus_tag="KPE55_002718"
     CDS             1282..2685
                     /locus_tag="KPE55_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493766.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            2712..4256
                     /locus_tag="KPE55_002719"
     CDS             2712..4256
                     /locus_tag="KPE55_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0493767.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            4287..5678
                     /locus_tag="KPE55_002720"
     CDS             4287..5678
                     /locus_tag="KPE55_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG0493768.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            5871..7367
                     /gene="abfD"
                     /locus_tag="KPE55_002721"
     CDS             5871..7367
                     /gene="abfD"
                     /locus_tag="KPE55_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG0493769.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            7502..7777
                     /locus_tag="KPE55_002722"
     CDS             7502..7777
                     /locus_tag="KPE55_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493770.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            7801..9108
                     /locus_tag="KPE55_002723"
     CDS             7801..9108
                     /locus_tag="KPE55_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0493771.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            9128..10246
                     /locus_tag="KPE55_002724"
     CDS             9128..10246
                     /locus_tag="KPE55_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0493772.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         3940 a         1180 c         2042 g         3225 t
ORIGIN      
        1 tatgaataga tgccatatac tcaataaaaa gtttcatact atgagatatg tattgatttc
       61 ttctccaaag aaccccaata ttcgcagtaa ttgcacgttc taatggtatt gaacagagat
      121 tttctgtatt ttgaaatatt tctttataca atattgttga agccaagtca ttttctagta
      181 aataacgtat ggtagaaagt tgatttgagt gtaaaattat atttggctta gctttttcaa
      241 gtctttttgt aataatctta tagtgataag aaccttcaga taagattaca aaaggtacat
      301 tttttaacat ataattcgat ataatgggat gttttgccaa ttcatttttg ggatttgtaa
      361 ctaagtataa ttctgtttta tagattgtct ttgaatctat atcatcataa atatcactgt
      421 caattatacc aatacaaaaa tcaagtgtag attcttcaat cattttagct ccaacaaatg
      481 ttggaacttc aaaaatttta agctctatat ctggataaat tacattaaat ctaggaagta
      541 tttttgaaag caaaaacgta cctaaaactg taggaatacc tattttgaga gaagcttttc
      601 tcttttttcc taaatcaagt gcgtcacatt gaaaatcctc aaattgtttt ataaaatgtt
      661 ttgacttgtc taaaaaaatt tttccttcag gcgttaactc tattttatta cctattctgt
      721 aaaaaagttc aaagccaagt tctttttcta attctttaat tgctaatgtt atggatggtt
      781 gagaaatatg aatactttcc gcagccttag tcatgttttt atatttacag acagtttgaa
      841 aatagtataa atagtttata ttcatttggt attagttcct ttcatttaca tattagtgac
      901 aagtatataa ttaacaatag cataagtttt gtatattgtt gtaaaaaaat agaatagttt
      961 taaactatca tagcataagt ttttgatatt ttcaatctat taagaatagt gctattatta
     1021 aattaacaaa agagaatagc tggtatggtt tagagtaaat caatctatat tacagttata
     1081 tcaacgaata tcaaaaaatt atctattaga aaataaaaat tatttttaca attaaattgt
     1141 taattaaatc ataaagaaaa aagtgatatt aacaagataa aattttttta atcagaaatg
     1201 ttaatttaat aacaaaatag agtattgggg agaaaggaga aatatatttt ttaataatat
     1261 ttgttaattt aataacgaaa aatgaaaggg agtgatttag tgaagtttga attgatggat
     1321 tttttaatga atccttttgt ccttatgttt gctgcagtaa ttacaggaat tttatttgga
     1381 aaaattaaat ttgggaagtt taattttggg gtatcaggag cattatttac aggattattt
     1441 attggatggc ttgcatatag cttggggaat ctaataatag caaaagggga aacagcagca
     1501 ggatataagg cagcaactgt aatgatgggt aatggaatta tttcttctga cttctttgat
     1561 ttctttttaa taatatttgt tgcagcagtt ggtctattgg cagcaaaaga tatgaaagct
     1621 gtattaaaga agtatggagc aagatttgta attttaggag ttcttataac ttttataggt
     1681 ggctctatga cttatgcaat gactttacta tcaagtgata aaggaagtag tgcttatgaa
     1741 gtttctggcg tttacacagg agcattaaca agttcgcctg gattagctgc tgctttagaa
     1801 acagctggta aacatgcaga agatgtatca aaagaatttg aaaaagcttc aataaaagat
     1861 aaaaaagaaa ttttaaaagt agtcgaccca gaaggcaaat tggatgtgaa tacaactaca
     1921 tcactgactc aagagcaaat agataagtat attgcatatg ctgaagctgg tgttggtata
     1981 ggtcatgcag tcgcatatcc ttttggagtt ttaatagtaa tcttaggtgt aaatttcctt
     2041 ccaaaattat ttaggatgga cctaaaagaa gaaagaagaa aatatgagaa ggaaatgaag
     2101 gaagcaagag atagtgtttc tggtaaaacg ataccagaag ttccatttaa tataataaca
     2161 tttttcttga catgtttagc tggatactta gttggaggaa tacatgtatt tatgggacca
     2221 ctagggtact tcacactagg tgcaacagga ggttcactaa ttgtatctct agttcttgga
     2281 tatataggta agataggggt agtaaatttc agaatggaag aaaaggtttt aaatatttta
     2341 aaacaaatag ggctagtatt tttcttagca atagttggat taagatatgg tggaaaagta
     2401 gttgattcaa ttatgacatc tggtatgcac ctagccttag tagcaatagc agtaggtgtt
     2461 acagctatga tgataggatt cttagttggc aaatacgtat ttaaattaaa ttggatttta
     2521 ttatctggag ctgtctgtgg tggtatgaca tcaacaccag gtcttggagc tgctgttgat
     2581 gctcttgata gtgatgaccc agcagcaggt tatggtgcta cgtatccatt tgcattatta
     2641 actaaagtta tattggttat agttttacac aaacttccaa tgtaaataag attttatatg
     2701 gggggatatt tatgaataat gaaggagcat ttgaagaatt aagtggaaga ttaagaaata
     2761 aagaattgtg tgatttaata atgaatgcag atgaagctgc taaaattata gaggatggca
     2821 tggttattgg agttagtgga tttacacctt caggctatcc aaaagcagta ccattagcag
     2881 tttcagaaag agctaaaagt ggagaaaaga taaaattaac cgtatattca ggagcatctt
     2941 taggtccaga agttgatgga gcatggtcag aagcaggtat aattgaaaga agacttcctt
     3001 atcaaactaa ttcaatactt agaaataata ttaataaagg tgttgttgat tatatagata
     3061 tgcacctaag tcattctact caatttttaa attatggaac tatacctaaa gtagatgtag
     3121 ctatagtaga agcacttgca ataactgaag aaggaaatat tataccaact agtgggatag
     3181 gtaattctcc atcttttata aaaagtgcag ataaagtaat agtagagata aatcttgcaa
     3241 aaccaatgga aatggaaggt atggcagata tatatattac ggaaaatcca ccaaatagaa
     3301 aaccaataga aataaatcat cctaaggata gaattggaac aacatatata ccttgtggtt
     3361 tggataaaat tgcaggagta gtaattacca atatgcaaga taagacccgt ccattgggag
     3421 ttgtagatga agcttcgaaa aaaatatcaa ataatattat tgctttttta agagaagaag
     3481 taaaggcagg cagattatca aagaatttac ttcctcttca atctggtgta ggaagtgtag
     3541 caaatgcagt attatatgga ttatgtgaat ctgaatttga aaatttaact tgttatacag
     3601 aggttgtaca ggactctatg ttggatttaa taagaatggg taaagttaca atggcatcta
     3661 ctacatctgt aagtccatct ccagaaggat taataaaatt tgaaaaagat attgattttt
     3721 ttaaggataa gattatatta agacctcaag aaattagtaa caatccagaa atagccagaa
     3781 gaattggtgt aattgcaatg aacactgcaa ttgaagttga tatttatgga aatgtaaatt
     3841 ctactcacat aatggggtca aagatgatga atggaattgg tggttctggt gattttgcta
     3901 gaaatggagc aataactata tttagtactg agtcaattgc aaaaaatggt gatatatcat
     3961 caatagtacc tatggtatct cacgtagacc acactgaaca tgatgtaatg gtaattgtaa
     4021 cagaacaagg atatgctgat ttaagaggtc ttgcacctag agaaagagct ataaaaataa
     4081 tagaaaattg tgcccatcca gattataaag aacaacttag ggattattta aatagagctt
     4141 gtaaaagtgg agcaaaacaa acacctcaca tattggacga agctttatca tggcatagta
     4201 aatttatgag tacaggtaca atgaaaaagg ctgaaacttt taaaagtgca ttataaaata
     4261 tttaataaat tggaggtaga ttttaaatgg aaaaagcagt ggaaaacttt gaagatttaa
     4321 gtaaggaata cattaatggt tatattgaga gagcaagaaa agctcaaaga gagtttgaat
     4381 gttatactca agaacaagta gataaaatag taaaaatagt tggaaaagta gtgtattata
     4441 atgctgaata tttagctaaa cttgctgtag aagagactgg aatgggagta tatgaagata
     4501 aggtagctaa aaataaaagc aaagcaaaag ttatatataa caatttaaaa gataaaaaat
     4561 cagttggtat aattgatata gatagagaaa ctggaataac aaaggtagct aaaccagttg
     4621 gagtagtagc ggcaataact ccttgtacaa atccaatcgt tacacctatg agcaatgcaa
     4681 tgtttgcact taagggaaga aatgcaatta ttatcacacc tcatcataaa gcaattggat
     4741 gtagtacaaa aactgtagag atgattaatg aagaattaga aaaaatagga gctccagaaa
     4801 atttaataca aatacttgac caacaatcaa gagaaaatac aagaaattta atttcatcag
     4861 cagatgtagt aattgccact ggaggaatgg gaatggtaaa agctgcgtac tcaagtggaa
     4921 aaccagcact tggtgtaggg gcaggaaatg ttcaatgtat catagataga gatgtagaca
     4981 ttaaagaagc agtacctaaa atcatagcag gaagaatttt tgataatggt atcatttgtt
     5041 caggtgaaca gtcagtaata gtagcagaag aaatgtttga taaaataatg gatgaattta
     5101 aaaataataa aggttttata gttagagata aagtacaaaa agaagctttc agaaatgcta
     5161 tgtttgtaaa taaatctatg aataaagatg cagtaggtca atcagttcat acaattgcaa
     5221 aaattgcagg tgttgagata ccagaagata caaaaataat agtaatagaa gctgatggac
     5281 caggagaaga ggatattatt gcaaaagaaa aaatgtgtcc agttatatct gcatacaagt
     5341 ataaaagttt tgaagaaggt gtagctattg caaaagcaaa tttaaatgta gaaggaaaag
     5401 gacatagtgt atctatacat tcaaatacag ttaaaaatat agaatatgca ggagaaaata
     5461 tagaagtatc aagatttgtt attaatcaat gttgtgctac tagtgctgga ggaagtttct
     5521 ttaatggtct tgcacctact aatactctag gttgtggttc ttggggaaat aacagcatct
     5581 ctgaaaatct tgattataag catctaatta atatttctag aattgcttat tatatgccag
     5641 aaaatgaagt ccctacagat gaagaacttt ggggataaaa tttagacaaa aaatattcat
     5701 tctaaattaa agtaggaata tattttaatt tagaatgaat ttaagacata aattaaattt
     5761 aaattagttt attggactaa atttaaaata ttaagagaca tatagaagaa ggaagtatta
     5821 taatttaaaa taaataaaaa aatctataaa agagaaggag tggttttatt atggcattaa
     5881 tgacaggagc tcaatatatt gaaagtttga gaaaattaaa tactaaggtt tatatgtttg
     5941 gtgaagaggt taagaattgg gttgaccatc ctatgattag accatctata aattgtgtag
     6001 cagcaactta tgacttagct catgacccag aatatgcaga tttaatgaca gttacttcaa
     6061 atatcacagg tgaaaaaata aatagatttg gtcatcttca tcaaagtgtt gatgatttga
     6121 taaaaaaagt aaaaatgcaa agactatgtg gtcaaaaaac agcttcttgt ttccaaagat
     6181 gtgttggtat ggatgcgttt aatgcagtgt attcaacaac atttgagtgt gataaagccc
     6241 atggaactaa ttatcatgat aattttgtaa aatacttaac ttatatacaa gaaaacgatt
     6301 tagtagttga tggagctatg actgacccta agggagatag aagtctatca cctagtgctc
     6361 aacctgaccc agatatgttc cttcatatag tagaaagaag agaagatggg ataattgtaa
     6421 gaggagcaaa ggcacatcaa actggttcaa taaattcaca tgagcacctt ataatgccta
     6481 ctataagtat gactgaagca gataaagact acgctgtatc atttgcagtt ccttcagatg
     6541 ctgaaggagt gtttatgatt tatggaagac aatcttgtga cactagaaaa ttagaagaag
     6601 gtgcagatgt tgatttagga aataaggagt ttggaggtca agaagcttta gtagtatttg
     6661 ataatgtatt tgttccaaat gatagaattt tcttatgtgg agaatgggat ttctctggta
     6721 tgttagtaga gagatttgca ggataccata gacaaagtta tggtggatgt aaagtaggag
     6781 taggtgatgt aataatagga gcagcagctt tagcagctga ttataatgga gcaaacaaag
     6841 cttcacatat aaaagataaa ctaatagaaa tgactcacct taatgaaagc ttatattgct
     6901 gtggtatagc atgttcatca gaaggtcaca aaacagaagc tggaaactat caaatagatt
     6961 tacttcttgc aaatgtgtgt aaacaaaatg taactagatt tccttatgaa atagtaagac
     7021 ttgcagaaga tatagctgga gggcttatgg ttactatgcc ttcagaaaaa gactttaaat
     7081 cagaccttaa agttggaaca agtggaatga ctattggcga agtatgtaac aagtatttta
     7141 aagcttcttc agtggcttca acagaagaaa gaatgagaat attaagattc ttagaaaata
     7201 tatgccttgg ttcatctgct gttggttata gaactgagtc aatgcatggg gcaggttcac
     7261 cacaagctca aagaataatg atttcaagac aaggaaacat aaatcaaaag aaagaattag
     7321 ctaagaaaat tgcgggaata aaaaaagagg aagctttaaa tttataaaaa gttgatatat
     7381 aaagggaaat tatatattaa tgtgtattaa ttaatgaaaa aatatgtagt ataagagata
     7441 gttataaaat tatggtggtt tttagctaag taaagaaatt tttaataagg agaatttaca
     7501 catgacctgt ggagttagat tttgtggggg ctgtaaccct agatacgaaa gagggaaggc
     7561 tcttacaata atcaaagagc acttcaatgg tagggtagat tttagtatag cacaagagaa
     7621 cgtcgagtat gatttgctac ttataattgg aggatgcgca actctatgcg catcctatca
     7681 acaatataaa gtgaaaaatg aatgtgttaa gatgtgggat tttagccata tagaaagagt
     7741 tataaatgtg attgaatcag caatttgtga aacttaaaaa agctaaatgg ggggatttca
     7801 ttgagttggc aagaattgta tcaaagtaaa ttatgtagtg ctacagaagc tgtaaaacag
     7861 attaaaaacg gtgatactgt agtatttgca cattgtgtag gtgaacctcc tgcactagta
     7921 gaggctatga ttgaaaatgc tgaacaatat aaagatgttg agattaaaca tatggtaagc
     7981 ttgggtagtg gtggatatac tgctaaggga atggaagctc attttagagt aaatccaatg
     8041 tttgtaagtg gaaatgtaag aaaggcaatt gaaaatggag atggtgattt tacacctgca
     8101 ttcttccatg aagtaccaaa gttgcttaga gaaaaaagat taaaatgtga tgttgtttta
     8161 gcacaggtaa ctccaccaga tgaacatggg tattgtagct taggaacatc agttgattat
     8221 acttatgaag ccattaaaac tgctagaaca gtaattgttc aagtgaatga ccagtttcct
     8281 agaacctatg gtgaggtagt ccatgtatca gagtttgact atatagtgga aaaatcacaa
     8341 cctttatttg aactacaacc tgcaaagatt ggagaagtag aagaagcaat tggtaaaaat
     8401 tgtgcttcat taattgaaga tggttctact ttacagctag ggattggtgg gataccagat
     8461 gcagttatgt tatttttaac tgataaaaag gatttaggga ttcattcaga aatgatttca
     8521 gatgggactt tggcccttta tgaaaaaggt gttataaatg gtaaatataa aaattttgat
     8581 aaagaaaaaa tgacagttac cttcttaatg ggtactaaaa aattatatga ctttgcaaat
     8641 aataaccctg cagtagaggt aaaaccagta gactatgtga atcatccagc aattataatg
     8701 aaacaacata agatggtttc tataaattct gctatacaag ttgatttaat gggacaggta
     8761 gttgcagagg ctatgggact tagacaattt agtggtgttg gaggtcaagt tgactttata
     8821 cgtggagtgt ctatgggtga agatggaaag gcaattatag ctatgccttc aataactacg
     8881 aaaaaagatg gtacagtaat ttcaaaaatc gtgtctattg tggatgaagg tgcacctata
     8941 acgacatcaa gaaatgatgt tgactatatt gtaacagaat acggtatagc agaattaaaa
     9001 ggaaaatctc ttagagaaag agcgagaaat ttaataaata tagctcatcc aagtgtaaga
     9061 gaaagtctag cagtagaatt tgaaaagaga tttaaggaga aatattaata atagaggggt
     9121 gttaataatg gaagcactta gagtagttcc aaagatattt tactttgata catttaaaga
     9181 atttaatgaa gaatttaaaa taggaaaaaa tgatttagta attactaatg agtttatata
     9241 tgagccatat atgaaacctc ttggaataga tacaaattta atttttcaag aaaagtttgg
     9301 aacaggtgaa ccatctgatg aaatgataga ctccatgaca aaggaaatga agaaatacaa
     9361 ttttgataga attattgcat ttggaggagg aacaatagtt gatatatgta agatattagc
     9421 acttgatgtt ccagaaaagt ctattgattt atttgaagga gaagtatcac ctaaaaaagt
     9481 aaaagagtta gtagtagttc ctacaacatg tggtacaggt agtgaagtaa caaatgtagc
     9541 tattgcagag ttaaaatcta aacatactaa aaaggggctt gcagtagaag aaacatatgc
     9601 agactatgca gtgttaattc cagagacagt aaaagggtta ccttacaaat tctttgttac
     9661 aagttcggta gatgctctta tacatgctat tgagtcttat ctttctccaa aagcaagtcc
     9721 atttacagag atgtattctc tacaagcaat aaaaatgatt atggatgggt ataagaagat
     9781 agttgataaa ggagaagaag agagatttaa tcatcttaga gactttgtac ttgcatcaaa
     9841 ttatgcagga attgcttttg gaaatgctgg ttgtgcagca gttcatgcac tatcttattc
     9901 aataggtgga gctttccatg ttgcacatgg tgaagcaaat tatcagttct ttactgaagt
     9961 atttaagatg tattctagaa aaaaacctaa tggaaaaatt ataaaatgta caaaaatttt
    10021 agcagatgcc cttgaatgtg accctaactg tgatgtttat ggagaattag aaaagttttt
    10081 aaataaatta atagctaaaa aagctttgag agaatatgga atggtggaaa gtcagataga
    10141 tgaatttaca gatagtacaa tagcaaatca acaacgacta cttgctaata attatgtaga
    10201 gcttagtaga gaggaaataa gagagatatt tgcaaatctt tattaataca attttttatt
    10261 tatatgtata gtcaacataa acaatgctaa ttatatttat gtacaaataa aaataaggga
    10321 acttaaattt taattaaagt taagttctct tatttttata tggagttaaa tataagtata
    10381 tttttaa
//
LOCUS       DAELNY010000131        10372 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.132, whole genome shotgun sequence.
ACCESSION   DAELNY010000131 DAELNY010000000
VERSION     DAELNY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.132"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            499..1164
                     /locus_tag="KPE55_002725"
     CDS             499..1164
                     /locus_tag="KPE55_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG0493773.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            1186..2118
                     /locus_tag="KPE55_002726"
     CDS             1186..2118
                     /locus_tag="KPE55_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG0493774.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            2139..2780
                     /gene="pcp"
                     /locus_tag="KPE55_002727"
     CDS             2139..2780
                     /gene="pcp"
                     /locus_tag="KPE55_002727"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG0493775.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2900..3232)
                     /locus_tag="KPE55_002728"
     CDS             complement(2900..3232)
                     /locus_tag="KPE55_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493776.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3498..4118
                     /locus_tag="KPE55_002729"
     CDS             3498..4118
                     /locus_tag="KPE55_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0493777.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(4443..5267)
                     /locus_tag="KPE55_002730"
     CDS             complement(4443..5267)
                     /locus_tag="KPE55_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG0493778.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(5278..6681)
                     /locus_tag="KPE55_002731"
     CDS             complement(5278..6681)
                     /locus_tag="KPE55_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493779.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(6685..7410)
                     /locus_tag="KPE55_002732"
     CDS             complement(6685..7410)
                     /locus_tag="KPE55_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493780.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(7522..8295)
                     /locus_tag="KPE55_002733"
     CDS             complement(7522..8295)
                     /locus_tag="KPE55_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493781.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIVFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(8301..9188)
                     /locus_tag="KPE55_002734"
     CDS             complement(8301..9188)
                     /locus_tag="KPE55_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493782.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(9202..10224)
                     /locus_tag="KPE55_002735"
     CDS             complement(9202..10224)
                     /locus_tag="KPE55_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493783.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFIAPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         3702 a         1579 c         1185 g         3906 t
ORIGIN      
        1 atcaaaattt tatttttctt agtgaaatct aatgaaacta gcacttaata tatgctttat
       61 taatcataac aaatatgttt tattaatcat aacaaaaaga gagtgtctaa tgaactaaaa
      121 aattcattag acactctctt tttgtatgaa atcaaatata tttttatcat ttcaacagag
      181 aaaaatacac ttgtctctat gtttggcaca ttctcttttt ataatataat gtttaattta
      241 aaactactat ttcaaatatt gacatatact taaactatag acttacaaaa tcaattcata
      301 ttagcttaaa atataatctt tgttattcaa attcctatac acaaccatta tttactttga
      361 tactttaaaa catcttccct attttttatt aaattttata ataatagtcc tattaacgca
      421 aatactataa aaagagttta actttatcca ttaaataaat ttaaattaaa acatatagaa
      481 atggaaggag aacaatttat gataaaacta atcggtatat tgatagttgt aattggattt
      541 atacttaaaa ttgacacact atttactgta ttattagcag gagtggcaac aggaattgtt
      601 gcaggattag attttaatca aatttttaca attttaggag attcttttgt ctcaaacaga
      661 ggtgtatcat tattcatatt aacattgcca gtgatcggtg tattagaaag atatggttta
      721 aaacaaagag ctgtttcttt aatagaaaaa ttaaaaagac ttacaacagg aaaagtactt
      781 actatataca tgatagctag acaaatagct ggtgctctat caataagaat gagtggacat
      841 cctcaatttg taagaccact tgttaatcct atggcacaag ctgctggttt aagtaatagt
      901 gatgagctaa aagaatcgga tgaagaagcc ataaaggcat tatctgctgc atcagaaaac
      961 tatggaaatt tttatggtca aaacttattt gcaggtagta gtggagtatt acttatagca
     1021 tctactctga ctcaatttgg atacaatgta actggactta atattgtaaa ggctagtact
     1081 attatggcag taatagcact tgtagtggct acaatccagt ttaccttata tgataaaaaa
     1141 ttaaacaaaa gtcataaaaa atagttttat ttaggaggaa taaaaatgca aaattttata
     1201 aacatatcat tagaaatctt ttacgcacta atgggatttt taatgattgt tatagcatat
     1261 aagtctttta ctaccataaa taacaataag aaatatggta catcattatt ttggatactt
     1321 atatcactgc cattcatatt tggtagattg attccagcca atataatagg aataatatta
     1381 atattatcta gtttattaac actttctaaa caagttgtat ttgcaaagta tgaagaacca
     1441 gatgaaaatt ttggaaaaga acaagcagac aaacttaaaa ataaaatatt tataccatca
     1501 ttaatattag catttgctgc agtagtagta gctatgagcc tttctaattt tgataattct
     1561 tctcaatttg caataggtgt gggttcaata attgcattaa tatcagccct tataataact
     1621 aaggcaaaac ctgctacatc agttcaagat ggctcaagat tacttcaaca aatgggacct
     1681 gctagtatgt taccacaact tttagttgca ttgggagctt tatttactca agccggagtc
     1741 ggtgaagtta tttcaaccat gatatctggt gtagtccctg ctgatagcag attatttggt
     1801 gttatagctt atgtattagg tatggttata tttaccatga taatgggaaa tgcatttgcc
     1861 gcattttcag tcataacggc aggaattggt attccttttg tattgtctca aggtggaaat
     1921 cctgctataa ttggagcatt agcattaaca gcaggatatt gtggaactct acttactcca
     1981 atggctgcta attttaatat agttccagca gccttgctag aatgtaaaaa tgattatatt
     2041 gttataaaat atcaagctcc agttgctttg gtattaatta tagcccacat attagtaatg
     2101 tactttttag gattttaata taattaggag gtaaaataat gaaaattttg ttaacaggat
     2161 ttgacccatt tggtggagaa ccaataaacc cagctcaaga agcagttgaa agggtcaata
     2221 ataatataaa tggtgcagaa atcataaaga taactatacc aacagttatg acaaaatctg
     2281 ttgaagctat agataaagct attcaagagc acaatccaga tatagtaata tcagttggac
     2341 aagctggagg tagatttgac attacacctg agagagttgc tataaatata gatgatttta
     2401 gaataaaaga taatgagggt aaccaggtta tagatactat tataaaagaa gatggtgaac
     2461 ctgcatattt ttcaaaattg cctgtaaaag ctatggtaaa acatatgaat gaaaataaaa
     2521 ttccagctag tgtttcaaac actgcaggaa catttgtttg caatcatgtg atgtatggaa
     2581 ttctttatat gatagacaag aaatatccta atataagggg tggatttata cacatcccat
     2641 atacaacttc acaagtaata gataaaaaaa atacaccatt catgtcttta gaagaaatag
     2701 taaaaggctt agaattagct atagaagctt gtattatata taaagaagat gtaaaagaaa
     2761 ttggtggtga aatttcataa taaatttttt agtattttta taaattaaaa atactaacta
     2821 ttaatttaaa tatagactgt gagtagatta ttcaaacaat tttactcaca gtctttttat
     2881 ggtatctaca gatacaaaat taaggtctat tcctctctcc ccactcacac attgcatcta
     2941 aaattggaat cagagatttt cccctttctg atagactata ttctacctta ggaggtattt
     3001 gaggatattc agtacgaata atcaagttat ctgcttctaa ttcttttaat gataaactca
     3061 aagttttata ggagatagta tttatataac gttttaatgc attatatctt acaacttcaa
     3121 attctacaag acagtaaaga attatcatct tatatttacc actaatcagt gaaagtgtat
     3181 aactaaatcc tgtttcactt aaatctgtat ttgatataca gtctatactc atatattaca
     3241 ctctcctttt ggttagtatt gaacaaaaat gtgcgtactt gtttttagaa taatttataa
     3301 taaaattata gtgtaagaat aattgaaagt caattttata ataggaggtg tatttactaa
     3361 aaaaaaagtt attttaaata ggattctaag attagacatt tctatataaa ttgataatat
     3421 tcatgaaaat cctattttat aaaaaataat atctcttaga aaatctatta aataaaattc
     3481 atgggaggaa atttataatg aaagttttat tgataaacgg tagcccaaac caatatggat
     3541 gtacttatac agcactaaat gagattacta aagtattgag taaacacgat attaaaacag
     3601 aaatactgta tttaggtaaa gaaacaatcc caggatgtat tagttgcgca tcctgttttg
     3661 aaactggaaa atgtattcga aatgataaag ttaatgaatt gattgaagat cttgataata
     3721 ttgatggtat tattattggt tctcctgttt acttttctag tgcaactggt caattaactt
     3781 cttttcttga ccgtctattt ttcatagctg gaagtcgcat ggctacaaaa ttaggtgctt
     3841 cagttgtgtc atgtagacgt ggtggtgcaa gtgcaacatt tgaccaacta aataaatatt
     3901 tttcaatcag caatatgcct attgtatcat cacagtattg gaatcaggta catggcttta
     3961 ctccagagga tgttatgaag gatgaagaag gattacagac tatgcgaact ctaggagaaa
     4021 atatggcatg gcttcttaag tgtatatcag caggtaaaaa agctggaatt aaagaaccac
     4081 aatatgaaga aacaattatg actaatttta taaaataaat aaaaaatgca ctaatattaa
     4141 ttaaaacaaa acactaattt ataaataaaa taagcataaa tgtaggataa aaagcactaa
     4201 aacagaggta ttgaatttta tatcaaacct ctgttttatt attacattaa taaccaaacc
     4261 atgtataaca tcatatattt ctaaataaat tataatatta tattctaatg ttattgaaat
     4321 tacaataact gaatttctgc tcataaattt aaatattcta caaatctcat ctaaataaca
     4381 attttaatag acctaaaaca atcctcatat tgttcatttt gtattaaaaa tatcacttac
     4441 aatcaaaaac catttattaa gtatattcct aagatataca atatatatac atggattgga
     4501 tagtataaat aaaataaata cttcatactc ttcccttttt ctccattgta aagtaacatt
     4561 aaaggtagag aagcaatcat catccattgg taattttgta aaatagaatt ttttaaacta
     4621 taatctccat ttgacattgt tgtaaatata aatatacaca ttaaaacata acaaatactc
     4681 aaactgaact ttttccctct acacaaataa aaaataatcc caagtataat ccaaattggt
     4741 ccaccttcta ctaaaaatgg tactggtacc aaaatcataa ataaagcaat aactctgtta
     4801 gtgactttaa aaagtaaagc tactgtaaat attcctaaaa ttataggaat gattatcatc
     4861 aataatccta taataaaata tttaatttct ttctcttttc taaatttctt tataaactct
     4921 attgcttgta aataaatcgc tattaaaaat aatgttgaaa aaatgttatt tataattata
     4981 gctccttctg gatgtgaaaa tacattatta attatttgat ttcctatagc cataataaca
     5041 gaccaaatat acaatctact tatatatttt tttctgttac tagtgtgata aaatccttca
     5101 acagccatga atataaacag tggtgctgat attctgccta cccaatgaaa ccaaattggt
     5161 atttgcattg tttgtggcat aaatgctgct atatggtcaa atgtcataaa tattaatgct
     5221 aaaatcttaa tagtaaaccc acttatacct tttttaaaaa aatccatatc atatctccta
     5281 attttgtttt atctctataa taatttttgt tcctattcca atttcactac ttacataaat
     5341 tttaccttta tgaatctcta ttatttcttt agaaatagcc ataccaagac ctgaaccttt
     5401 atgcatctca cctgtattag tacctctata atatttttta aaaatatgtt tcaaatcact
     5461 tttgcttatt ccaattccat tatcttttat tataatttct atattatctt tttttacaat
     5521 ttctacactt atcaaagttc cttcgctatt atgaactatt gaattaaata taagattggt
     5581 tatagccctt ctgaataaga ttgaatctac atatacttca attaaatctt cattgctttc
     5641 aaattcaata ttcctatttt tatatctatt atcatttaaa atatcaatta ttatatttct
     5701 gactaaactg actaaattta tttttttctt gtccagaact atagtattat tttttaatcg
     5761 agtagataaa tttaaatcat caactaaatc ttttatatac ttagacttat tatatataat
     5821 ttctgtatac tcttgtattt catcttcttc aaactcataa tctttgtcat taattatctc
     5881 tgcataccct tgtatagatg ctagaggtgt ctttatatca tgtgttatat ttgccagcca
     5941 ttcctctctc aactcttcat tttcttttct ctcaacttca tttactctta aagtatcagc
     6001 taacatattt atatttttaa aaacttcttc atatatacct ctgtccttaa gatataaact
     6061 ataatttcca ttagccatag tctcaatact ccatagtacg cttgaaattg gttttgtaag
     6121 ttttctacta aataaatatc ctacgcctaa agctaccacg ctatcaatta ctagtgttat
     6181 aatgataact ttatttaaag tttttttaat gttattttga gaataggtta aaattattct
     6241 tgatatatct ctactaggta tacctactat tatgtttaaa tgctttttgt ttaaatattt
     6301 ttctagtaca aaattaacat actttgtttc tatatacttg taattattca ccatttgaaa
     6361 gggtgtatat tttttgggag tatcctttgg cacatttact ccataaactt cctctcctaa
     6421 atcatttaaa acctgtatcc aggaatttga tttttctaat ttttcagctc caatcttaga
     6481 tagtttaaat tctccatctt tttcatataa atctttttca aaagaacgtg caaattcttc
     6541 aggattattt ccagaatcta caactttaaa aaagctatta gttgaaatca catacaatat
     6601 agatatgata tttataatta ctactgatat agctacaaac acaattgtaa atataaaatt
     6661 tctagtaatc ttccacttca tattctaatc ttcctttact gctaatttat atccaagacc
     6721 ttttattgtc agaatatatt caggttttga aggattatcc tctaattttt ccctaatttt
     6781 tcttatatga accattatag tattgtcaaa tccaacataa tcttctcccc acactttatc
     6841 ccatagtttt tctttactta taatttgatt ttgattatgt gcaaataaag ataggatttt
     6901 tagttccttt gcagacagtc ctatagattt tccatctttt ataagttctg ccctactttc
     6961 atttaatata tatgggccaa atgatataat tttttcttca ttattattta gatttttact
     7021 ttcgttttga gcatcactaa aataacttaa tcttctcaaa tgcgctttaa ctctaaaagc
     7081 cacttcctta gggctaaaag gttttgtaac ataatcatct cctccaatcg ctagtccaag
     7141 taacttatct aattcttcgg ttttagctga taaaaatatt attggagaat tggaggtttt
     7201 tcttatttct ttacaaatat cataaccttc gccatctggt aacataatat ccaatacaat
     7261 caaatcagga ttgatacttt taaataattc taatccttct tttttagttt ttgcagtata
     7321 tatattatta agaccctctt tttttaggac agtttctaaa agcttcaata tttctacttc
     7381 atcatctatt attaaaacac gattttccat aaacaacctc ctgtaataat ttacatatat
     7441 attagcatat attttatcta aaattcattt atcttatttt taattaaact aaaatataaa
     7501 taaaattact attattttta gttaaaacct tttgcttgat gtaaaatttt catatgttat
     7561 tttagttaac actatcatta atataattga gattactata gaaaaaacta tcaaaatact
     7621 tggtacatta tacttaacca tttgttggct catcataatc caatttggag tcactgacat
     7681 aaattttata gctttatctg gtataaaacc tacactaaag tatggaattg ccattatcaa
     7741 tccacctagt ataaatggaa ttacagccga tttaaatata gatgaaagtg ttaagcctat
     7801 ttgtgcaaat ataaaagaag caaaaatagt agtcaatata acaattatat acatttgtat
     7861 tactgtaaga tttaaattac tatttccaaa gtaccataag ttatgaatcg attcatttaa
     7921 atttttaaaa tcaaaaaaac taaaacctct aattcctact aacaatataa aagaaatatt
     7981 aataatagat aaaattaatc cattagctac tagttttgat aatactactg atttttttcc
     8041 ctttttagtt gtatttatta aacctgtcat attagaatca tattctttaa tgtaatttcc
     8101 agaagttatt ataaacatta tcaaacctat tagaggtatt gagccttcta tcttgtaaaa
     8161 tatatctgca taagtatcta aaacatcaaa ctttctaatc agaaaaaatc caaaaactat
     8221 taaaaacagc caaattatcc aaacactctt tgattttaat atttttatca tttcagcttt
     8281 aactaaatac atcatttcta ctacacctcc cttgcatctt ctaaatcaaa ataaaacatg
     8341 tatacatctt cgaattttgc ttgaactggt tgtatattat cctcgcttgg aatatcctct
     8401 gaaacaatcc taagtactat tgaatcaata tcactttgaa tacttacaac cttatattta
     8461 gattgtatat catcaacttc acattcattt ttaacttgaa cattatatac tttattttcc
     8521 atgtgactta atatgtcttt gtgtgttcct ttcattagga gtttgccatc tttaatcatt
     8581 attgtttcct ttgcaactga ttctatatcg gaaattatat gagtagaaag tattattatc
     8641 ttatctctag acatttttgc tattaaattc ctaaatctag ctctttcttg tgggtcaaga
     8701 cctgctgttg gctcatcaag gactattatc ttagggtcgt ttagtaaaac ttgtgctatc
     8761 ccaactctcc tcttcatacc tcctgaaaat tttcctatag acttatttct cacatcataa
     8821 agacctacta aatctaataa ttctcctatc ttactattta aatattttcc ccttaaacct
     8881 tttaaagctc caatatactg taaaaattct tttgctgtaa aatttttata aactccaaat
     8941 tcttgtggca aatatcctaa aatatttcta taatcagcat ccattttaaa tatatcctca
     9001 ccattataaa gtatctgacc ttcatcaggt tttatcaacg ttgttatttg tttcattaat
     9061 gtagattttc ctgctccatt tggtcctaga agaccataaa ctccattttc tagagttact
     9121 gtaatcttgt cgtttgctac ttttttacca tatatctttg aaagattttc tatttctaat
     9181 ttattcatct taactcccct tttatctact tatataatct gattttatta ctaaaaaaca
     9241 taatgacata gcacctatca atattgccat caaaagtcct attgctatat tgacaatgtc
     9301 tatattcact ttaaatatca taatatcacc tagataattt cctattattt tattaaaacc
     9361 aaatatcaaa tcataataat ttacatttga tacaactgaa agtaattttt taggtaatat
     9421 atggattacc gacattatct tagttataga tataataaat ataaaaaaac ttattgaaga
     9481 tattgaagac ttacttataa aagacccaaa tatggctatc atggcccatc cttcaaacat
     9541 caaaagctct atgcctatat ttaatattac atactttgtt atacttaaat tagttttcaa
     9601 tgcaactaca ttgtctacta ttctaaaaga ttctaagtct ccattaacag gtactccttg
     9661 ttgcatacaa gttataataa atattcccat taaataaaca gaataaatta ctaatggaac
     9721 taataaagca acaaatattt tagactttaa tgcttttttc ttctcttttg atgatagtat
     9781 gatatctttt accgaagtaa cgacttcatc tacgtaaata ttggatataa ttatagatat
     9841 aatgattaac attcctacat ttataagtga atttgtaact ctatagttaa atactttcca
     9901 aaaactaata tccttatatt cattcacact tagattatca agttttttct ttgacattaa
     9961 ttcaattttt ttagaaaatt cacctgtatc tgtttgattt gaaaactgct ttaaaacatt
    10021 aatcttattt tgaaatttct cattacctac gtcgatacca cttctcgtat cttctatacg
    10081 tcctttttct ttatctatat aataactttc tgtttctaaa acaggcgcaa taaagatact
    10141 aatcattagt attaacacta atatccctag agatataaac atactcttag atttagccaa
    10201 tttttttatt tcccaattta ccaatatatt tctcctccta aatcatttta attacacata
    10261 tatattaaaa cttaaatctt atataaatat aatactaacc ttaaataaag cttaaattat
    10321 tttataattt ataggtgtgt ttattattgg agttacattt attatttttc tg
//
LOCUS       DAELNY010000132        10250 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.133, whole genome shotgun sequence.
ACCESSION   DAELNY010000132 DAELNY010000000
VERSION     DAELNY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.133"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(203..1453)
                     /locus_tag="KPE55_002736"
     CDS             complement(203..1453)
                     /locus_tag="KPE55_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG0493784.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1485..2426)
                     /locus_tag="KPE55_002737"
     CDS             complement(1485..2426)
                     /locus_tag="KPE55_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG0493785.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2666..3742)
                     /locus_tag="KPE55_002738"
     CDS             complement(2666..3742)
                     /locus_tag="KPE55_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0493786.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4115..4630)
                     /locus_tag="KPE55_002739"
     CDS             complement(4115..4630)
                     /locus_tag="KPE55_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG0493787.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4993..6063)
                     /gene="leuB"
                     /locus_tag="KPE55_002740"
     CDS             complement(4993..6063)
                     /gene="leuB"
                     /locus_tag="KPE55_002740"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG0493788.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6070..6561)
                     /gene="leuD"
                     /locus_tag="KPE55_002741"
     CDS             complement(6070..6561)
                     /gene="leuD"
                     /locus_tag="KPE55_002741"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG0493789.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6576..7853)
                     /gene="leuC"
                     /locus_tag="KPE55_002742"
     CDS             complement(6576..7853)
                     /gene="leuC"
                     /locus_tag="KPE55_002742"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG0493790.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7810..9552)
                     /gene="leuA"
                     /locus_tag="KPE55_002743"
     CDS             complement(7810..9552)
                     /gene="leuA"
                     /locus_tag="KPE55_002743"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG0493791.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9676..9922)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10070..>10250)
                     /locus_tag="KPE55_002744"
     CDS             complement(10070..>10250)
                     /locus_tag="KPE55_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493792.1"
                     /translation="SKQSQKFNYKIIEKIEVVKNILNIYTDESNYKIRLRALSLEDEK
                     KLLNIIDEKMKKFKA"
BASE COUNT         3081 a         1988 c         1067 g         4114 t
ORIGIN      
        1 atatacaaat acaattatat gaattttcat cctcaattat agcatagaat atgactatct
       61 atatcctaaa attatccatt tctaaaaaaa aggtaactat tattaagaat atcaacttat
      121 agaatttata ttccacatac gattttcaat caaaaaaaca agggctaaaa agtgaattgt
      181 gcacttttta gccctttatt aattattcat ttatttcatg taagaataga ccacatctta
      241 ctttaggttc aaaccatgtt gacttagctg gcattatctt atttgcatct gctactctta
      301 ttaaatcttc aatactagta ggatacattg caaatgctac tttcatatct tcattaactc
      361 ttttttctaa ttcttcaaga cctctaattc caccgataaa atcaattctt ttatctactc
      421 taggattatc aattcctaaa atagcatcaa taacatagtc ttgtaatata gaaatgtcta
      481 atgagtcaac agggtcttct atttctaata aatttttatt tgctttcatt ttataccact
      541 tatcacctag gtacattccg aaagttcctt ttttgtcagg tttatatttt tcttttcctt
      601 ctatttcttc tagttcaaaa ttttctttta ttttatttat aaattcttcc tcgcttaaac
      661 catttaagtc tttgataacc ctattataat ccaaaaccat taaattctca tctggtgcaa
      721 tcattgctat gaagtaatta aattcttctt ttccagtata atttggattt ttagctctca
      781 tttctttggc tacattttct gctgaagctg tcctatgatg accatctgca atgtatagat
      841 aatctaattt ttcaaattta tctactaggt tatctattat atcatcatta tcaatcttcc
      901 atactgtatg ctctatcttg tcatctgtta tgaaatcata taaaggttca ttattatcca
      961 taaaatcatt gattatatca tctatcaccc tttgatgttt gtaagttaca agtatagttc
     1021 ccgtattagc atcacagtat tttatgtgtt tagttctatc aatttccttt tctggtcgag
     1081 tgtactcatg tttttttatt gttccattca agctttcatc aactgatata catgctacta
     1141 taccaacttg agctctacca tccatgattt gtttatatat atatatacac tctttgtcat
     1201 cttttactag aatttcttgt tttcgaaact catctaaatt ttctcgcgct tttaaataaa
     1261 ctttttcatc atataaatct atattttcat ctaaatcaat ttctgattta tctatatgca
     1321 aaaatgaata tggattccct tctgcaattc ttctagcttc tttactattc ataacatcgt
     1381 aaggaagtgc tgctacttta tctactatat atttatttgg tctaatcgcc ttaaatggtt
     1441 taatcgttgc cattactaat ttcctcctaa ttatacaaaa tattttataa tgctacccct
     1501 attagattgt tgaagtcaag aaagaaatct tgaatcacat ttactatttc gtctcctatt
     1561 ctttcttgag cttctactgt agatgcccca atatgaggtg taggagaaac tcttggatga
     1621 tttactaagt ctaaatttaa tgtaggttct tgttcataaa catctatagc agctgctgat
     1681 aatttacctt catctaatgc ttttagtaaa tctttttcat caactaaacc gcctcttgca
     1741 cagtttatta aataagcccc tttcttcatc ttatttattt ctttttcagt tattactgcc
     1801 cccttattct tatcaaatgg tatgtgtaaa gataagaaat ctgcattttc taaaacttct
     1861 tccatatcac agaatttata attgttaaag ccttgcgcct cacccattat gtcagtatat
     1921 ataacattca tacctaataa ttctgctctc tttgcaactt cttttgcaat tcttccaaaa
     1981 cctataagtc caagtgtctt tccattaatt tctgtacctt tatacttttt cttctcccat
     2041 tttccatctc tcatagttac atttgcagta tttatgaatc ttgcaagaac aaataattga
     2101 cctattgtaa gctctgcaac agatatactg cttgcatttg gagtattcat aacttttatg
     2161 ccattagctt gagcatattt aacatctata ttatccagac ctactccacc tcttactatc
     2221 aacttcaact ttttaccctc taatgcttta tctattacat ctttagtaac tttagtagct
     2281 gaacgtacta caagtacatc aacatcttta agtttatctc ctaaaacatc tttttcataa
     2341 aactgctcta tcacattaaa atctaactct ctcaattttc tcgctgcttc tttctctatt
     2401 ccatcagtaa ctaatatatt atacataaca aactcctcca atttttattc acttaatttt
     2461 tccaattttt attttttaaa gtattttcat caataaaaaa ctttacttaa ttcaactttc
     2521 tgtttatata attttcaaaa tattttttta ttaaagtcat gtcaaatttt tctattgttt
     2581 gttaaaaatt taatatgtaa tttttataaa atattattta ttatatattc taaatttatc
     2641 ttaaaacata catattttta caatattatt tattaaaacc taatatatca tcaatattat
     2701 ctaataattc ttgtacatct tcgacagtat agtcacccat atgtgaaatt ctaaaagttt
     2761 tttcttttaa atctccatag ccatttgcta tttggaatcc tcgttcttgt aattttgaat
     2821 ttaaatttga tacactaata ccttgagtat tttctataac agtaagtgta ttggataagt
     2881 gattttcatt ggtaaaaatt tggaagtgtt ttttagccca gtttctaact aagtttgcca
     2941 tgtcttcatg tctgttgaat ctgttatcta ggccttcatc taatatatta tctaattgga
     3001 aatctaaagc gaacatgtga gaaagagatg gtgttgaagg gtattggtag tttttcttga
     3061 ttaagtattc atacattgct aacaaatcaa gatatactcc tctaaaaggt acttgcttag
     3121 ctctatcgat tgctttttga gaaaatgtgc aaatagacat tcctggtggt aatcccagtg
     3181 ctttttgtac tgatgttata caaatatcta ttccaatctt atcaacctcg acttttattc
     3241 caccggctga acttacagta tctacacagt agattacatc ttcatacttt ttaacaactt
     3301 ctccaatttc ttctattgga tttcttattc cagttgaagt ttcattgtga gttacagtta
     3361 ttaaatcgta ttctccggta gaaagcacct tatcaaccat ttctggcgtt gttgcctctc
     3421 ctaattcaac tttaaatata tccgctggga cattattagc cactgccatt ttgtaccatc
     3481 tatcaccaaa ggatccacag gaaaatactg ctgccttttt ggatgtacag gaacgaatgg
     3541 agccttccat taaaccagtt ccggatgacg tggataaaag tatcgtgttg tttgtataga
     3601 atagtttttg catcttttca cttatactct tttggagcat agatgcatct tttcctctat
     3661 gtcctatcat tggtgtagcc atcttttgca agacttcttc tttaacatca ataggaccag
     3721 gaataaataa tttcttactc attataattc cccccttttt agtttgtaat atggatagcg
     3781 tttttactgt ccttattttt aataactata atatcaagct gaattaacta tgtcaacata
     3841 aaatttattt tttatatata atttatttta tatattttat gaaaacattc attttctacc
     3901 ttttcataag tctgaatttt ctgaattatt ctctcaaaaa aaatatactt tttatctata
     3961 taaattaatt tttttagtat gtaaatttga cgctattacg ataaatcttt ttagtataac
     4021 taatttattt ttatcttgta tttttattgt atttttgaat tttatttttt ctaaatataa
     4081 aatttatgtt ttaaaatgta aaatttttat ttaatcaaag ttcaatatat tccacttttt
     4141 ctttcgattc ttcaaaaaac cattcttcat tagaaaaatc accttctgca atttgtccaa
     4201 taggcatttt catatacttt ccagttgctg tcactgctat atctccattt ggtagaatta
     4261 tttctcctgt accttcaaat aattttctgt tttctttagt tattcttcca acaactttta
     4321 tagtagcatc tgttggtact ggtttcttat atttcaactc taaagatact gttactcccc
     4381 atatttggtc atcatttata cttacagctc ttccaatagt ctcatccaat attgcagcag
     4441 acatacctcc atgaactctc ccaggataac tttgatgcca atcctttgta ttacaaattg
     4501 atacaagctc tccattttca agttcataaa aatctgcttt aagtcctagc tcattttgtg
     4561 ttccacaaac taaacaagat ttactactat tttgcttctt caaaacttta tactttgcca
     4621 aaaaatccat ctcctaaccg acattagtag tcgcttaata ttaaataatt ctaaaatata
     4681 ttatataata ttaataaaat ccctgcaaac ttctaataat aaaaataggc tacttcaata
     4741 tgataagttt aatttaaagt attattttcc aaaaaataac tttatcaaaa tagaaactca
     4801 aagtagatat aagcctcttt ttcgttatta aaatagtaaa aatatatttt acttaatata
     4861 aaaaagagtg tctcattaac taataaagtt tattttgaga cacccctcat aaaacaaatt
     4921 atactacaat agatttttac taattctaca agttatatat tcaaattata ttattcaaat
     4981 taaactataa ttctaaattt catttactat taattcgccc atttcccttg ttcctacaac
     5041 atttcccaca ccattataga tatcaatagt tctataacct ttattgagta cactttgtac
     5101 agcatcttca atacacttag cttcttcttc taagttaaaa ctatgtctta acatcatgct
     5161 tacagataga atagtcgcaa ttggatttac aatatctttt ccagctatat caggtgcact
     5221 tccatgtatt ggttcataca taccaaaact atcttctcta agtgatgctg atggtaacat
     5281 acctatagaa cccgtaatca tagatgcttc atcagatatt atatctccaa atatattatt
     5341 agtcactatt acatcaaatt ggctagggtc ttttacaact tgcatagcag cattatcaac
     5401 atacatatgt gacagttcta catcttcaaa ctctttagct aagtcttcca ttatacttct
     5461 ccaaagtctt gaactctcaa gcacatttgc cttatctaca cttataagtt ttttatttct
     5521 atttctggct atttcaaaag ctctcttacc aattcttctt atttcatttt cgttatatat
     5581 ttctacatca taagcatttt cgataccatc aatgattttt cttccacgtt ctccaaaata
     5641 aatgccccct gttaattccc ttacaacaac aaaatcaatg cctttctcaa ctatatccgt
     5701 tctaagtggt gaagcttctt ttatactttc atacattgta gctggtctta aatttgcaaa
     5761 taaacccaat gactctctta attttaaaag accagactct ggtcttttat ctcccttaca
     5821 tttatcccac tttggtcctc caacagcacc aagcaataca gcttcgtttt ttcgacaagt
     5881 ttcaacagtt ttattaggta gaggttctcc agtttcatca atcgcgcatc ctccagctaa
     5941 tacatattca caatcgaatc tatgattaaa tttacaacaa attttattta gaacctttat
     6001 accttcatct ataatttccg gtccaacacc atcacctttt atgactgcta tattacaatt
     6061 catacttcac taccccctat ttgcttttat tgagttaatt aagccttcat ttaatataat
     6121 attttgcata aactcaggaa atggttctgc tttatattct tttccttttg ttatattttt
     6181 tatcacacct gtagaaaaat ctacttctat attatcccct tcatctatgt tattggcagc
     6241 ttcttcacat tcaagtatag gaagccctat atttatagag tttctaaaaa atattctagc
     6301 aaaagtagaa gcaatcacac aggatactcc actttctttt attacaattg gtgcatgttc
     6361 tcttgatgaa ccacaaccaa agtttttagt tgcaactata atatctcctt tttttacctt
     6421 agaaataaat ttatcatcaa tatcctccat acaatgcgtt gctagttctt tataatcagc
     6481 tatatttaaa tatctagctg gtattattac atctgtatct atattgtcac cgaatttaaa
     6541 aacacttcca ttggcaatca taaattttac ctcccttaaa ttatttctga tggttttgat
     6601 atttttccag ttatagctga tgctgctgct acagctgggc ttgcaagata cacctctgaa
     6661 tcaacatgcc ccattcttcc tacgaaattc ctatttgttg ttgaaatgga tttttctcct
     6721 gctgcaagta tacccatatg tccccctaga catggaccac aagttggtgt agaaactgct
     6781 gctcctgcct ctataaaagt ggtaatatac cctttttcta tagcttcaag atatactttt
     6841 tgagttccag gaaatatcat aactctaact ccatctgcta cttttttgcc tttcattatc
     6901 tcagctacta cttcaaggtc tgatattctt ccatttgtac aagagcctat tacaacttgg
     6961 tctatcttta tatccttatc tatctcatct actgttctag tattttcagg aagatgagga
     7021 aaggctactg tttcttttag ttcagctaaa tctatttcgt atacttcatc atagactgca
     7081 tcttcatctg cttcatatat atttacatct tttgtcatag ttgaacactt atgtgaattt
     7141 atatattcca tagtcttatc gtctactgga aaaattccat tttttgctcc tgcttctatt
     7201 gccatattac aaatagtaaa tctatcatcc atagataagt attctactcc atctccacaa
     7261 aattccattg acttgtaaag tgctccatca acacctatct cccctattat atgaagtatt
     7321 atatctttac cacttatcca tttatttggt ttattcttca aaacaaattt tattgctcca
     7381 ggaaccttaa accaacattt tcctgtagcc atacctgctc ccatatcagt tgaacctaca
     7441 ccagtagaaa atgctcctaa tgctccatat gtgcaagtat gagagtcagc acctataatt
     7501 acatctccag cagttactat acctttttca ggaagaagaa catgctctat tcccatatct
     7561 cctacatcaa aaaaatttac aactcctttt gattttgcga aacttcttgt aaatttacat
     7621 tgttctgcac tctttatatc tttattaggt gtaaagtggt ctaaaaccat cactacttta
     7681 gtcttatcat aaacatcttc aattcctaat ttttcaaatt ctctaatggc tactggtgat
     7741 gttatatcat tacctaaaac tacatctaaa tctgcttcga ttaattgccc tttcttaact
     7801 tcatttaatt tagcatgttt ggctaatatt ttctgcgtca tagtcatccc cattttttat
     7861 ttcctcctca atataacttt tatttattgc attcataagt gcattaatag ctgctgtatt
     7921 tatgttttcc gatatactta caccaaaata gacttcatta ttgtgctcta ttcctacata
     7981 agctacccct cttgaatgtg acccttcctc taatgcatgt tcccagtaat atctaaattt
     8041 ataatctttc atgccacatt gctttagtgc attattaaat gaatctacag gtccatttcc
     8101 tataccaaca atcctttgtt catttcccat ataacttatt cttgcagtca tctcaatcat
     8161 gttagtatca ttagaatcat cattttcata gtttaattca tccattgatt ttattttata
     8221 cttcttaact ttataagggc tctcaacttc aatatattct ttcttaaaca aattaaatat
     8281 tgcttcacta ctaagttctt ccccaattct atcagattcc attttgacta catttccaaa
     8341 atcagaatgc atattcttag gtatcataaa tccataatct gtttccatta tataaacagc
     8401 tcctcctttt ccagattggc tatttatcct tataatttct ttgtattctc ttccaatatc
     8461 atgagggtct attggtaaat atggaacttc ccagtaatca ttatctctgt ttttcattgc
     8521 tatcatgcct tttctaatag catcttgatg tgaacctgaa aaagcacaat gtacaagatt
     8581 acctgcatat ggatgtctat catgtaccat aagttttgta cagtcttcat atatacctat
     8641 tatcttatct atattgctaa aatctagctc tgggtctatt ccttgtgtat aaagattaag
     8701 tcccacattt actatatcca tattcccagt tctttcacca tttccaaata gtgttccttc
     8761 aagtctatcg gcaccagcca ataatccaag ttcacttgca gccgtacaag ttcctctatc
     8821 attgtgagta tgcaaactta gtataataga atccctacaa cttatatttt tacaaaacca
     8881 ttctatttgg tctgcatata cgtttggtgt tgccatttca actgttgatg gaagatttat
     8941 tattactttt ttttgaggag ttggtttcca aacatctatc actgcctcac atatttcaag
     9001 tgcataatcc atctctgttc ctgtaaaact ctctggtgaa tattcaaaag taaattctgt
     9061 ttctggatat ttttttactt cttcattaaa taattttgct ccttcaactg ctatatctat
     9121 tatttcttgt ttactcatat taaaaaccac atctctttga agaacagatg tagagttata
     9181 taaatgtata attgcttttt tacatcccct tattgattca aatgtttttt ctataagatg
     9241 agccctagat tgtgtaagta cttgtatagt tacatcatct ggaatcatat tctcatctat
     9301 taattttctt aaaaatgtat actctgtatt tgatgctgat ggaaatccta cctctatttc
     9361 tttaaatcct gtatcaataa gcattttgaa cattctgact ttttcattta cactcatagg
     9421 tgttggcagt gactgatttc cgtctcttaa atcgacacta caccaaattg gagccttaaa
     9481 tatttcatta tctggccatt tacgatttac tatttgcatt ttatcatatt ttttatactt
     9541 tccacacttc atgttatttc cccctttttt tgttttttgt attcagtata cctcatatcg
     9601 acgcaatcct gcacaattca taaggataaa tgtcaatttt atcattagaa taaaaaaaac
     9661 ctcatctcta ataaaattag agacgaggtt ttgctcgcgg taccactcta tttagctttc
     9721 taaaaagctc ccttagtaat agccaataca ataattataa tgactatatt tcctataacg
     9781 tggaattact ccgattattc taagatttaa atcttagaaa acagctccta gacaagttca
     9841 gaactatcaa ctactaaatc acaccatcct ttagctctct gaaagtttca ttgttcttac
     9901 tacttctatt catcactttt ttaatcattt aaattttcta aatattttga tttaatagtt
     9961 attatataca cattaaaatg tattgtcaac acaaaaaata attatctcaa ttttttattt
    10021 ttgataagca attctataaa acatgctaac tattgaaaac agtatgatat tatgccttaa
    10081 actttttcat cttttcatct atgatattca ataatttttt ttcatcttcc aaactcaatg
    10141 cccttaatct aattttataa ttagattcgt cagtgtatat atttaatata ttttttacta
    10201 cttctatttt ttctattatc ttataattaa acttttgact ttgtttagat
//
LOCUS       DAELNY010000133        10239 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.134, whole genome shotgun sequence.
ACCESSION   DAELNY010000133 DAELNY010000000
VERSION     DAELNY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.134"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..632)
                     /locus_tag="KPE55_002745"
     CDS             complement(<1..632)
                     /locus_tag="KPE55_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG0493793.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISG"
     gene            complement(613..906)
                     /locus_tag="KPE55_002746"
     CDS             complement(613..906)
                     /locus_tag="KPE55_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0493794.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            1299..1349
                     /locus_tag="KPE55_002747"
     CDS             1299..1349
                     /locus_tag="KPE55_002747"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493795.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(1875..2627)
                     /locus_tag="KPE55_002748"
     CDS             complement(1875..2627)
                     /locus_tag="KPE55_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0493796.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(2686..4341)
                     /locus_tag="KPE55_002749"
     CDS             complement(2686..4341)
                     /locus_tag="KPE55_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0493797.1"
                     /translation="MVDFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(5614..6693)
                     /locus_tag="KPE55_002750"
     CDS             complement(5614..6693)
                     /locus_tag="KPE55_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0493798.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNQNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(6777..7433)
                     /locus_tag="KPE55_002751"
     CDS             complement(6777..7433)
                     /locus_tag="KPE55_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493799.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            7755..7961
                     /locus_tag="KPE55_002752"
     CDS             7755..7961
                     /locus_tag="KPE55_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493800.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8114..8542)
                     /locus_tag="KPE55_002753"
     CDS             complement(8114..8542)
                     /locus_tag="KPE55_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0493801.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9018..9956)
                     /locus_tag="KPE55_002754"
     CDS             complement(9018..9956)
                     /locus_tag="KPE55_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0493802.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
BASE COUNT         3405 a         1818 c         1090 g         3926 t
ORIGIN      
        1 ttacctgaaa ttttatacat caaataagaa tatataaatg gtagtattac aattacaaag
       61 attacaaaga tattataaaa aggaatatta ataaatgcat taataattaa taatattcca
      121 ccaataagcc aaatccatcc agctaaatgg tgtgtcttat tccaattatc ttcatcatta
      181 agagtccatg gcaaaattat tcctactgag taatttcgtt tacatttagg aagatagttt
      241 ccaaataaaa taactattat tcctaaaatt aatcctgtat aaaaattaga ctccaaacta
      301 actcctgttg aatataaaac catcattatt tgtataatta ctgaaagtat agcaactccc
      361 cattttgata aattttgtat aacatttgaa aaattagctt tcttaggctc attctcaagt
      421 ctaaaccatg tatacaaatg gatacatagc attaacaatg gtacaccaaa agctgctata
      481 acctttggag cataattatc tggatttcca taattatcaa aatgaattgc aacttcattt
      541 ggtaatctat tataaaaata tattgataca ataaatggta taaaacatat taaagtagat
      601 aaaataaatt gtttatttat cgcttttttc attatctttt tctcccttct ccataaattg
      661 tgaaaaccaa agcataacct cttcgaaaac tgaaatattt atttcgtaga aaataaagtt
      721 cttatacttt gtttcaaata tgagtcctgc cttttttagt tgagatagat gataagaaat
      781 tgtagcattc gtcatattaa atttttcact aatttctcct gctgacattt tattctccct
      841 caacataacc aatatttctc ttcttatagg gtctgaaaga gctttgaatg tttctggaaa
      901 ccccaacacc atcacctcgc tatttagaat ttcatctaaa tagaatataa cataacatcc
      961 attttatttc aatagctatt tagaaatttt tctaaatagc tatttctaaa tagttattga
     1021 aaattttcat attaagattc tcaatttact taagtcgctt ttatttaatc ttactttatc
     1081 taattttttt aacttaacaa aagtgtattg caccataatt gttaaagcaa aaacctaatg
     1141 actgagatac ttatatatat aacaatactt ttaattttaa attaaaagta gctaaaccca
     1201 atttaaaaag tagttaagtc ctatttaaat agaaaagaat gaaacgagaa atatactgta
     1261 taaaagtaca taaaaacatc tactaattag atacaactat aacagaatat atttattatt
     1321 ataataacaa aagaataaaa aaaaattaat ttagcataac cttgttaaat atagatttga
     1381 atatgaattt aaagttatgt aaaattaaaa atacaggaat gaaaattcca ttcctgtata
     1441 aaatctaact tttgagtctc agtacaatct tattttgaga caatcttttt tgttttatat
     1501 tttcttatat ttaaaatata acgcatatct tatgcaacat attcatattt ttctttatat
     1561 tctttcatcg catcattcca agcgtcttgt actgttacac ctcttttatt agcaattttt
     1621 tgtgccagtt caattatctt catagctctt cttgatatgt tcataaacat tcctcctaca
     1681 aattaccata tatttcatat atataaatca aggttatatt tttatgatat ataatccttc
     1741 tattgtaaga ttacagttta tatgtgtatt atatatgaac tatattagaa gataattaga
     1801 atttttataa agctatatga ttagattaaa tataaaagct ttacaattac ttataaattt
     1861 aaaaagtcta taagctaaac taatgtttga tataccaatt ttacgaccat aaataatgcc
     1921 attactaaaa atattggttt tatcatcttt gaacctttct ttatagcaac tttagcacct
     1981 acataagctc ccattatcat cactaatgca aacactatag caattttata atctacttga
     2041 ccatttatca tgaataataa caatgaagtt atattacttg ttaagtttaa tatttttgta
     2101 tttgcagaag catgtaaaaa atcatatcca tatattttta taaatccaaa ggtcaaaaaa
     2161 gttcctgtac ctggaccaaa aaaaccatca taaaaaccca ttataagagc cataagcatc
     2221 cctagtctta aatttttctt attaacagct tcaaaattat cttcttgacc taaattttta
     2281 gaaataaagg tgtaaatagc cactattaaa atcataacca caaccaaaac atttaatatc
     2341 tcttcactta ttgataatac acatctcaca cctagaacac taccaataat agtaaatggt
     2401 actaattttt ttaaaaggtc gttattaatt tttccagact ttgcatatct ataggcacta
     2461 cttatacatc ctgctgatgc tgcaaattta tttgtaccaa tggcaagatg tactggcact
     2521 ccaattgcca taagaacagg catgcttata agtcctcctc ctcctgctat agaatctaca
     2581 aaagcagcta aaaacccccc tatacataaa aaaataactg aaaacataaa attcctccat
     2641 cattctcttt caatttttct tacaatatat attatataat cttatttaat acttattatc
     2701 cagcatattc tttctatatt catttggagt tatactatta aacttcttaa atatttgtga
     2761 aaaataacta ctgttattaa aacctacttt cttacaaata tcactaatac tcatgcttct
     2821 atctaacaga tattttttag attcttctat cctaagaaga tttatatact cagttattga
     2881 aactttattt ctttcattaa aaatagaact caaataattt ggacttataa aaaaaacatc
     2941 tgatatacta ttcaaattta tattttcttt ataatatttt tttatatatt cgtttatatt
     3001 ttcaattaga tttcctttat cttttctcca aaatgctcta ttcttatcaa tacactcttt
     3061 ggaaaattca aataatacat tccataaatc ttcatcactg tttacaagtg atattttttt
     3121 attatattta tttcttaaat aagatataag ttttaaatcc tctaaatctc taagcactgt
     3181 tctagacaat acaaatatca tttcctgaat agtatctcta acatcattat atcttaattt
     3241 gtttgtatct attagattat aaacaccttc taaattcata tttttaattt ctttttcaat
     3301 ataaagttct aattcaaagg gatattcttt tgaagaatta ttgaacatat ctatcggatt
     3361 aataatatac tcttcttcta ataatctact atttgctttt ttaagtgcct cttcaagtct
     3421 tatattttct tttctactct tcaatatctc ctcatctttt atactaagtt gattttcaaa
     3481 aaaattcctc ttagctattt cctgaataaa ttcagacatc attaataaat tttcttttaa
     3541 tacatataac ctacttttag gatttaatgg aaattcattg taaattttgt ttatgtattt
     3601 tatatcaata ccagaactac ttgataattc agataaattt ttatctatat caatagactt
     3661 atatactata aaattaccac attctatata tccaactata ttttcttcta atactatagg
     3721 tatctctaaa ctggaaacac catactcaca atggtacact ttttcaaagt tatcattatt
     3781 tatgctttta acaagttttt tagtacactg ttttatattt ttacaaatat cgcaaaattt
     3841 attttctttc ttagtctcaa atatttcttt atactttaag tcatatacag atattctaag
     3901 cttcaatata ttagatattt gatttgcaag attttcaatt atgtttacat taactaactc
     3961 ctgtatattg ttttcagaaa atactatagg tatttgtctc aaactcgaaa atttatgagg
     4021 aacataagta attaatatta ctaaatcaat tttaccagtg tttataattt catgttctga
     4081 atatggtggg caataaacta tagatgtttc ctttatattg acctttttac catcaattat
     4141 ttgtattcct tctccctgaa tcacatataa aagctgttct tcacttaaat gaaaatgctt
     4201 ttcttggcag tttcccggaa aaaatcttac aattccagca ctaagtctat tgaattgtgt
     4261 ttttgatggt tcgtgtatcc acataacctc tccccaatca aatttttgta cttcattatt
     4321 tgactcacta aaatccacca taaactcacc tcgtagtttt tgtctaaata ttttttcttc
     4381 ccttcttata ttaaaataat atcatcatca ttaactttca ttactataaa atcatcatac
     4441 tcttttaata ctcctgtaat tttagttgca cttagtttac tacaattcag ttttttaata
     4501 tattgataaa ttctataatt acttttttat tttgtaaaat cagtgttttc ccaaaataat
     4561 tatatctata aatgaaagta tttttactgc ttacatcaat atatgttatc ataattttcc
     4621 tttttattgc atatataaat aactgtatat aacattgtcc aatactatat agaaatacat
     4681 ctaagaaaga atattattaa attgttccaa ttagtattat ttttcttata aaaataaaaa
     4741 aactgttgga ttcttttaga aaaattattt tttacatacc ttttttgctt agatagtcta
     4801 tcttctcagc tattttgtat atacactctt ctagttcatg attttatttt aatatcttgc
     4861 ttatattgct gaaataattg aattctttta tctatcatat tgtctcttat atcataaaca
     4921 tattgtttct tatatcacaa aaatgaaagc attcttatat cttcaatata taaacctgct
     4981 ttttttaatc ttgtaataaa tataggatac tctatattct cttcctagaa ataactaata
     5041 ccacttattt ttctattata tagaaagtaa taaatctttt tcttaataaa attcaatagt
     5101 aagtggaata aaattttata tgaataagaa cattataaaa atactttata caatagtcaa
     5161 taaaagatat tagcttcaaa ttatgttaaa gttgtattat ttaaagtgaa aaaacaaata
     5221 aaaaagaaat aatagtataa gtagaaaaat atacttaaaa caaggcgaag cctctcttgt
     5281 atctaaatca agtagagaat taaatctaaa aaagaaaata aatataaaag atgactcctg
     5341 tacattacaa gaatcatctt atggtttcat aatcactcca tggtttttaa ctgtctgata
     5401 taaaggtctc agttcactca tagcatgacc accctttccg attgctgacc ccgtcactta
     5461 tggacaaaat gtccataagt gatagtcctc tcttaaaacc ctctaaatag atatatctca
     5521 agcacctcat tgaaaccagt tgctggaaac aaactgaagc gttctggaat agtaaaagaa
     5581 attgccacca tatccacgtt actgtcctag ttgttacttt gctaacttcg gagcaatttc
     5641 ctctaacaat acgcagtcaa attctgcctt tttgctaaga aatggcagat ggtctgcatc
     5701 tggtataaaa aaaatctgtt tgtctggcgc cactatttca tcaaaaaatt tcttcgctgg
     5761 aagatagggg ctattatagt cattttctcc catgatgtag tatacaggga cctgatagtc
     5821 aactccataa tttcttatgt cagcttcttc cactaaaaac cgcaatacat caccttgatt
     5881 gtcaatcgca ttttgattga caatggccgt attttttaac tctctcaatg aataatacgg
     5941 ggttgccagt atactgatta tattgcccaa atccatattt atagaaagcc catacttgct
     6001 ttggtatgtt cttactgtca taagagctgc caaatagctt ggaccatatt ttgctggcgg
     6061 atatggtgca atagcttcca gcgccttgat atctttttgc ttccctgcag ctttcaccat
     6121 ttcaagtgtc ttttcatagc ccacgcgctc attgtccacc atattaacgg tctgcgcaat
     6181 cccaatgtac gcgctgtaat cctccggata ggtttgcaca gccatagttc ccagcactgt
     6241 tccccagcta tgacctataa ggataatttt ctccttgttg aactcttgct tcaaatatgc
     6301 tgtcacctca tggacgtctt gcagaacacg ctccgcgctc atggtctgca aaactgctgc
     6361 cggatcgttc gcaaagtatg ttttgcctac gtttcgctga tcccagttca ccacagtaaa
     6421 atcattttcc cactcatact ggtacagatg taaaaatggc atcattgaat taccaggacc
     6481 cccatgaacc caaaggatga tggggttgtc tgcgttttgt ccccggatat ataacgcctg
     6541 ctgtatgccg ccgatctcca ccatttccat gcggtcgatt ccaggagctt ctattttatg
     6601 gcttgtacgt ataatcgtgg gggctatcag acgcaaaccg gcaaacgcca gaacaaaaac
     6661 agcaactagt atgcaaagtg tgattaatat cattttaagt accttctttc atgaattttt
     6721 ttagccatag atatctcctc taacattaaa aaaaatatgt tgctatttta gttttgttac
     6781 tctacctgtc ccattggttt cctttcataa aaaatattaa ttgattctat aatctcatct
     6841 tccaggatat caactcctat ttgtctcata agaaactgta agaattgaaa ccgtttacga
     6901 agtgtaggaa agtccccctg aaatacatct gtatcacacc aaaagttaaa gagcagcaaa
     6961 attacctctg cgcattcatc tgggaactct gtgatgatgc ttctatcctc tatgccttct
     7021 cgtaaaacat ttgctaatat tggtgctagt tttttcaaat tatcctgtgt aaatgccagt
     7081 atgatatgcg gactttctat agcgcttgta atcatgttac tagattcttt tatcacctca
     7141 tccgttaaat gccgcctaac taaacttcta agcttctctt ttgctgtaag tccatgcatt
     7201 tcaccgagcc attgatttac tgtctctatt atctgttcaa agcgcctttc catgaccaca
     7261 ttaaaaatat cttcttttga actgaagtga tgaaaaattc cgccctttga cattcccgaa
     7321 gcatcaacaa tattttgcat acttgttttg tcatatcctt tttcagcaaa caattttgca
     7381 gaaatggtaa tgatattttc aatagcttgc tttggattgt tctttctagt cattatataa
     7441 ctccttctta ttaaaaaccg actgttggtt tttattatat aagctattcc tacttctgtc
     7501 aagcaacatt atgtttatgg caaaacaata aggtttggtg gttagacagg tattaattag
     7561 actaatgcga ctttccccta gatggaaccc ttggtaaatt tgcaatcttt accggctttg
     7621 attatatctg taagcaaggc aggtatggcc acacctgccc tgtttgaccc tcggatattc
     7681 attcctatcc cttctctcct tagccattac accaaaacgc cgctcattgt ctccaatggc
     7741 ggcgttttaa agatatgatt tctcatgctg ctgaaatatg caaaaaatat aagatgccct
     7801 ttattatttt tcctacaata gaaaagaaaa gtattgaaga atgggttaat attggagcaa
     7861 atatgctttg tttggcacaa gatactacct ttatcactta tgttatcaat caattattat
     7921 ctttttataa tgagattgta ccaattcaaa aacaacatta aattataaat ctaattttat
     7981 attaaaagag actaatattt tatctatctc tgttaaggat attttcttat gtatcataat
     8041 attagtctct cttattatta tatactgcta tcatcattag ttgctattta tactattttt
     8101 ataatataca aatttattca gctaacgttt tgcctaattc tatacactca tcttttgctt
     8161 cttcatttgg gtcttccatt gctgtaattc cttctccacc aataattgta gcaccagcat
     8221 tttgcatacg ttcttcccag tctctcatcc attcacaatt tccccatcca tatgaaccaa
     8281 ataatccaat ctgtttacct gataccatag attctaattc tgaaacaaat ggctccatat
     8341 acccctcttc tagttgctct gctcccatag atgggcaacc taatgcaaat ttatcttctt
     8401 cttttaaatc acttggtttc agtaagctca catctaaaac ttctggtgtc ttacctttta
     8461 acttcacacc ttcagccaca aaatttgcca ttttttcagt atttcctgtt ccactccaat
     8521 aaacaatata tattttactc atttttacct cctgtagtat tttttaatta atcaatagtt
     8581 agttatagct aactaacatc taaaaaacaa aagatttctt tatctgaaat ctctttattt
     8641 ttctaaatat agaatatcat agttgatttt gaaattcaat gcttattgat aatatttatc
     8701 atttatattt tatattcatt ttataatatc aaaacactgc atcgtatttc taatctttca
     8761 tagaaaaatt tctaacttat agtacttttg catctcttat aaatctttta tatgtataag
     8821 tacatagaaa aacacacctt ttccagatgt gctatctata atatcgtata tttatttttc
     8881 aattttaact agctaatatg tttataaatt ttatcactta atacctttat acattgatgt
     8941 aaaatcttac acctaagtaa tttttattat ttgtctcgac tctatataat atagctattt
     9001 tatcatcaaa tcaaaaatta tcttttgcca ttattcttgt atttatagag atttaaatat
     9061 aagtatccat ttccttgttc cgttctaggt attccaagcg ctctcgtaca ttttattaat
     9121 tgtgcataca gttcagcttc atctaaatct ctttcaaact cctctcttgc ccattcttta
     9181 attagtgcta atgagcctga tacgtatggt gcactcatac tggtaccact cataatagca
     9241 agtttattat ccatataagt cgatataata tttcttcctg gagctaccaa atctattgta
     9301 gtatttgaat tactaaactt ttcaaccaaa tagttttcat ttattgcacc tacttctatt
     9361 acctcagcat aactggctgg ataactatac tcacttgtac tagaatcacc atctccatta
     9421 ttacctgctg cacacactac agaaatatta ttttttactg cttgcatgac agcatttttt
     9481 aaattcttat catctttgtt tccaccaaga gacatagata taatatcaac cttgttattt
     9541 acagcaaagt taatagcatt aattatactt tgatatgttc cagtaccatc tttatttaat
     9601 gcttttgcta ttaataattt acaatctgga gctactccca taacttcatt attatagtta
     9661 gatgcagcta taatacccgc cacatgagtt ccatgaccat taaaatcctc atatatattt
     9721 ttatttccat tactgtcatc actaaaattt gcaccaccaa ttatttttcc ttttaaaaga
     9781 ggatgagata tatcacaacc tgtatctatt ataccaacta caatattttt accagtataa
     9841 ccttcatccc acattcccct agcattcatt tgttttattc catatgggaa tttatttttt
     9901 gcacctctta gattttcatt tatttcatac ggaattagtt tcattctttt attcatcata
     9961 tatcctccta aaaattttat tgcttataaa aatcatgaat tttattttat aataggttta
    10021 taaatcaaat taaaacctat atctacttat ttatatttat gatatatttt ttatttttag
    10081 gagtcatatt ttattgtata taaaataata tttagataac tatattaaaa aatacatgtt
    10141 tgcttatata caaaacttac taaaatgtct ttaatatttt tgtatacaaa cattatattt
    10201 tttatataaa aagctcataa aaattttgat tatattttc
//
LOCUS       DAELNY010000134        10232 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.135, whole genome shotgun sequence.
ACCESSION   DAELNY010000134 DAELNY010000000
VERSION     DAELNY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.135"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..885
                     /locus_tag="KPE55_002755"
     CDS             106..885
                     /locus_tag="KPE55_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0493803.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1195..2016)
                     /locus_tag="KPE55_002756"
     CDS             complement(1195..2016)
                     /locus_tag="KPE55_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG0493804.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2750..3568)
                     /locus_tag="KPE55_002757"
     CDS             complement(2750..3568)
                     /locus_tag="KPE55_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0493805.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKTVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3736..4317
                     /locus_tag="KPE55_002758"
     CDS             3736..4317
                     /locus_tag="KPE55_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493806.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4314..4508
                     /locus_tag="KPE55_002759"
     CDS             4314..4508
                     /locus_tag="KPE55_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493807.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4672..6414)
                     /locus_tag="KPE55_002760"
     CDS             complement(4672..6414)
                     /locus_tag="KPE55_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG0493808.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGNDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6843..8219
                     /locus_tag="KPE55_002761"
     CDS             6843..8219
                     /locus_tag="KPE55_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG0493809.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8506..9177
                     /locus_tag="KPE55_002762"
     CDS             8506..9177
                     /locus_tag="KPE55_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG0493810.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9366..10163
                     /locus_tag="KPE55_002763"
     CDS             9366..10163
                     /locus_tag="KPE55_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG0493811.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3769 a         1242 c         1572 g         3649 t
ORIGIN      
        1 aatatctcaa ataatatgaa tataacatct gaatagaggg tgttttattt gtaaaaaata
       61 agtttacagt ttgagcgtat aaacttatgg taaaggagaa taattatgag ttataagaag
      121 attgcaatta taacgggtgc taatagtggt tttggtaaag agtttctaaa gttattaata
      181 aatgaagaag aaataacaga aatatgggca gttgcaagaa acaaggcgag attaaatcaa
      241 ttagtagatg aatttggaag taaggtaaaa attttttcaa aagatttatc aaaaatagaa
      301 gaagtaaaag aaattggtac atttttaagt aaagaaaatg tatgtataaa ataccttata
      361 aacaatgctg gttttgcaaa attctgttca tacaatgatt taagtataga tgaatctatt
      421 aatatgattg atttaaatat tagtgctgtt gtagcattgg gacttatatg tattccatat
      481 atgcaaaagg gaagccatat catgaatatt tcttcacaag catcattcca gccattgcca
      541 tatcaaaata tttatagttc aactaaagcg tttataagaa attatacaag agcattaaat
      601 gttgaactta agagtaaagg gataaatgca attgctgtat gtccaggttg gatgagtaca
      661 aatctattta agagaggtat agttgtagct gaaaaaggga caaaaaattt cccaggcata
      721 gtgtctccag acgttgttgc caaaaaagca ttgaaggatg caaagaaaaa taaggatatt
      781 tctgtgtatg gtataaatac aaaatccagt catttattag caaaactatt gccacaaaaa
      841 atgatgatga aggtttggtt aatgcaacaa catattaagg aataagagtt ttggtaatta
      901 agtatcagtt ggagattcaa tctgttatat attatgagaa gttttgttga gcaaataata
      961 attgtgacaa aacggcttat ctagtgacga taagccgtct ataatgtata taaaatatat
     1021 ttttgatttt actttattta gatattgaag tattaaaata tgtaggactt gaaattttat
     1081 aaagataaat actatgatat tttgtaaaaa ctgcgatatg ttgatatcac atagtttgac
     1141 tatactgtta aattgaagtt ttcttatttt gcattatgct tattacttaa tgatttagac
     1201 tataatttcc ataccatcat atgcaaaata aatgttatca agtttctttt ctaattcact
     1261 gtaataacca tatgattttc cccacatttc gtcaatatgt gtaacaataa tttttttaat
     1321 tctataatag tttttaattt caacaatctc atctaaagta aatatttcat ctttaaatgg
     1381 tgtgtcttta agttttgtac catcatttaa tataccatca tcagaaacta gacttattat
     1441 taaaatatca gcatcaaagt acatatcact atgagtaaat ggcttgggat tacagcaggc
     1501 atatatgact tttttttcat tctctttgat aacatagaag gttatattcc tgtctgggtt
     1561 attgttaatc aaatcaatat caaaattatt tatctttaca tgcgaagttt cattaataga
     1621 aatgcaattt ataacatcac cataatattt aagacaatcg agattcgttc ggtttatatc
     1681 ttcaattact tcaggaagcg cataaaagcc aattggatta ttttctcctg tgataaagtc
     1741 acatcctatt ttttcgacaa ttctaatacc cttaacatgg tctgggtcat ggtgagaaat
     1801 tgaaatatgt tttacttccg atattttttg tctgttacaa gctactgcaa tgtcttcagg
     1861 tgtgtctatc agcattttta tatcatcaat atataaactt ggtcctaatc gttcatatct
     1921 tccgcctttt tgtctggctt cttcacatat tttgcatttg caaaatgaac ttggaataat
     1981 tgacataccg ccactaccaa caatttttaa tttcaaagtt attacaccac cttctaaaaa
     2041 tattagatta tataaataat ctacaatcac aactgattaa caaaattatg agttaataaa
     2101 ttatatcaca tgtaaatatg aaagaatagt atgaaatttt tataagcata tattaaatat
     2161 ttctcaatga aattgacttt gtatatgaac cgaatgactt attcaatatg gataagacat
     2221 ttttcttgta ataagatatg attgtcctat aaggtttatg taattttgat ataatttgat
     2281 ataatatatt gattaacaac atacatgtta ttggtgagca tatttctata gaatggaata
     2341 tataattttt tatatttatc attgtattat tgtacaaaga aagttaaaaa gagtagagta
     2401 caaaggaatt atttgtactt tactctttgt tttgttaaaa actaatttat attagtgatt
     2461 caaatattta actagagtat gagggttgta tgtaatctta taagtaattt atctgacaaa
     2521 ttaagataat atcccaatca aattctagtc atttaaacat aacatcacaa gtgatttatc
     2581 tgacaaatta agataatatc ctaattaaat tgtagatatt tatacataac actaaaagta
     2641 atttgtttaa caaattaaga taatatccta atcaaattgt aggtatttat acataacact
     2701 aaaagtaatt tgtctaacaa attaaaatat tatccaatta atataaaaat tacttctttt
     2761 taactgggaa gcatacctct gttacccaat tatcaggatt actatcgttg ccaggactta
     2821 catgataaat attaaacata ggaccatcca catcatagtt attatctgat atccaattac
     2881 caatagcctc acaagcgtct gcaacttgat tgaaatttcc attaacaatt gcagttgcag
     2941 cagtcacact tggaacagtc ttaaatttaa catgttcagt atctttatac tttccagata
     3001 cagcaacttg tacttcaaca tctacataat cttctttata tccagtatca taaaaaactg
     3061 ttttagaata ctttgggggt tcaatttgaa cattctggtc ttttgtctct aaaaatgctt
     3121 gatgccataa cattccttcg tcttgataag ttggtataac ccttctaagt gtcatcatat
     3181 attttggtgc aaaattcttt atagttacat cataattttt cataatatca tttcctccaa
     3241 ttcttttaat agtggtttct atttttaata gttttttctg tgtatcttct aattgttctt
     3301 ttacttgaga atggtggata tttagatatt ttatgagact ttctttatcg ttatattctg
     3361 tcaaaatttc ttttatagaa tacaagccaa agcccatatc ttttagtgca tggattctgt
     3421 ttgtagtaga aagttggtct gcactatagt atctatatcc attagtttta ttcgtatgag
     3481 aaggagttaa aagtcctatt tcatcataat gacgtaacat acgaatacta attttactta
     3541 gctttgaaaa atcgcctatc ttaaacatgt ttttgtctcc tttataaagg tacttttagc
     3601 ttaatattaa tataaaccat atcataatgt tagagtcaac taaaattata attaaattat
     3661 attgacatat gatgaaaaaa atattatact gaacatgttc agaactgaaa atgttaattg
     3721 ttttggaggt ctattatgaa aagtaatgat aaagatgcta gagaacgtat aattgaagtt
     3781 acattgaatc ttttaaatga agtagatgat attgaggaga ttacagttag aaaaattgct
     3841 gagcgtgcaa atgttggagt tggtttaata aattatcatt ttaaaacaaa ggataatttg
     3901 ttgagtactg ccataggaga tgtgatgtca aatataatag cagagctata tgacgatagt
     3961 gtatatactc taagacctat tgaagattta aaaaatttat taaaaaaatt gtgtgatact
     4021 ggattacatt atgaaaaagt gcttcctttt gtattgaatc aatgtataac caatggagat
     4081 atgcaagcag aacttgatat tgtccctatg cttcgtaaaa tttttggaaa taaaaaagat
     4141 gaaatgagtc taagaatcat tgccctgcaa attattttac caatacagat ttctgcttta
     4201 tctacagaat catttcagtt atacagtggt ataaatataa agaataaata tgagcgagat
     4261 aaattcattg atattttaat agaaaatatt attggagagg atgttgatgt aagatgaaaa
     4321 ttataactat tggttcaagt cttatcactg ttttactttt tctttcaact atggtttgtg
     4381 ggttctggat taagaataat aaggtaacag atgcaagttc tattaagttc catatgaata
     4441 gtgctatttt tacagggata tttttattga tttccacaat ccttttaatt atatatataa
     4501 aaaaataata atattattaa taaataaagt atcagaaatt ataaagacaa gagtcttaaa
     4561 aatttctgat actttaccat ataattttat taaacattaa ttgtgtgtga aaaaatatca
     4621 cttatttcaa tctaatacta aatcttaaga gtgagtataa agtattagaa attaagaagc
     4681 attttttagt tcaagtggat tatctatacc aaaataatca tataaagtag agcttatttc
     4741 ctctaaattt ccattttgga aaggtaattg taaatttacc ttttctacca catctttaaa
     4801 tccagcatca gtattttgat ttaaaaaatc catatataca tcaacagctt ttctgtaatc
     4861 ttcttgtgat aattgccata tctgaagtga tacatcagat gcaagaccat aactcaagta
     4921 ataaaaagga acttggaagt tatgagatac aagtatccaa aaaggagcat ttttttctct
     4981 aagtatatta ttggaaactc catattcttc ctctaaatca aaaaacaatt tattgatatc
     5041 atttagactc atatatggat tcttgtatat aatttcttga aattcatcat aaagacaagc
     5101 atcaataata gtatttaaaa caatcgataa gtgttctttt tttatagctt cagattgttt
     5161 accaaaaaaa tcatcaaaat atttataaaa tagaatttct aaagatgttg aatgaacttc
     5221 acatacatct atacttggtt gcatcttatt atttaagtta atactgttgt agtaattata
     5281 aaaatgacca aactcatgag caaaactagt aacacctaaa aaagaattgt cccatgtatt
     5341 aaacaagaaa ggtgctttat atttatttat ataagtagta tatcctccag gtgatttgtt
     5401 ttttccagaa gaataatcgt acaagtcata ttttttcata tagtcgaagc tctcttttag
     5461 ttttggagat atatcttcaa gtactttatc aaacttccta aatgagcgat ttttatatac
     5521 tttaatataa atagaagaat cagatggttt agatgaaatc tctctatata atggaactat
     5581 gtaatctttt acatcttgtc taaatttttt agcttcttct tgagaatagt ctttgtttag
     5641 gttcatatag gcataatcta tataattctt aaatccttgc ttacttgcta tttcagttct
     5701 tgtctgtata agttctaaga atatgttacc aaatatagga ttgtattttt ttaagtaatc
     5761 tgaataaatt ttcacatatt cttcagggct aagatttggt cttgacaagg cttcttcaaa
     5821 gtctacctct ataccatcta ttgatactgt agatttagct aataagtctt catattttac
     5881 aactaggtct tgctcttttt taagcaaacc ttcaacttct ttagaattaa gttttccaat
     5941 gttctttaga taattaaatg tatcatttcc aagttctttt tctaattctt tagagaattt
     6001 agaagttaca aatctatctt gaaagtcttt gtatgacatg tcaacatcta ctgatttact
     6061 cataagatac ttatattcct tagagtaaaa ttcgtctgat gaatctatgt tatttctaat
     6121 ttttgctata gtaagtgtac tgtaaaaatc ctgataaatt ttatcaactt cattaaataa
     6181 atttaattgt tccttagctg ttttagctgt taaaagtttg tcgttacatt tgttaatgtt
     6241 ttcgcaaatt gactgtatat taggacgctt atattccatt ttttcaaaag gaatagttcc
     6301 tctaacagaa tctttttttg tatcattagt ataatttctt atgtatgatg caaatataat
     6361 aaaaactgca attaaaatta gaaaaacaaa tattttcttc ttggatttaa ccataaaaat
     6421 cctccaatca tggatatagt tattaattat tttactatat taaatataaa aaatgcatgt
     6481 taagaaaatt taacataaag atatagataa gattataaat aatataaaaa atttaacttc
     6541 aaaaaagtat atgaaattta tactggtaaa atgataaata aattttaagt acaaaatagt
     6601 aaaagctatg atagttttag acctattata tgtaaaaaat atagatgttt ttggtgcaat
     6661 ttaaattaaa agatatatat aaaaatttaa aatattccaa acagaaatta taaaaattac
     6721 tatcattgtc tttatacaat atttatacta taatagaata ataatttaga ggaaagctct
     6781 aaaaataaac aaattaaggg tcttaacagt agtgatttta tgttaaggag gtataaagaa
     6841 agttgaaaac aatagttaga agacttattc tctacgcaga tggaatgcgt cctactcaaa
     6901 taatggtatc tgggtttgct gcaattattg taatcggagc attgttgtta acactcccga
     6961 tagcgtcgca gagtggagag agtataggct tattaaatgc tctatttacg gctacttcag
     7021 cggtttgtgt tactggtcta gttatggtag atacagcaac ttattggagt ttatttggac
     7081 agatagttat aataacattg atacaaattg gtggtcttgg atttatgacc gtagccacaa
     7141 tgttttcact tatggcacga aaaaaaatac aactaagaga aagattatta atacaagagt
     7201 cattaaatca agctgattta tcgggacttg taagacttac aagatttgta ttaataataa
     7261 ccataacaat agagggaata ggtgcactgg ttttatcaac tgtctttatt ccacaatttg
     7321 gtttgtctaa agggatatgg tatagtgtat ttcacgcaat atcagcgttt tgtaatgccg
     7381 gatttgactt gatgggcagt gtaagtggcc catttacatc gttaaattct tatgtaaaca
     7441 actttacagt atcaatgact gtttgcgcac ttatagtgct tggagggcta ggattccctg
     7501 ttgtacttga tattgtaaga aaaagaagat tttcaaaatt aaatgtgcat tctaaggttg
     7561 tattgttttc aactgctaca cttatttttg tgggtgcatt atttattttt cttatagaat
     7621 ttaatcaaaa agctactatg gcggacttgc ctttaaaggg aaaagtacta tcagcaatat
     7681 ttcaatcagt aacagcaaga actgctggtt ttaatacact tgatttagct actttacgag
     7741 aaagtagtgt atttgtaatg ataattttga tgtttattgg agcatcacct gcatcaactg
     7801 gtgggggtat aaaaacaaca acactagctg ttttaatcat tacagttaga agttttctat
     7861 caggaaaatc agatatagaa gcttttgaaa gaagattagc tccatcaact ataaaaaaat
     7921 ctcttggtat atttgtaatt agtatatcag cagttatttt tggaacattg attatttcaa
     7981 taactcagcc taattttaca ttagttcaat cagcttttga ggttacatca gcacttgcta
     8041 cagttggttc aagtttagct ggaactccaa acttaaatgc tttaggtaag attataataa
     8101 taatttttat gtttatgggt agagttggtt ctcttacttt atttatggct atactttctg
     8161 gtggtagaag aaagagtcaa ccaatcagat atgcagaagg taaaattatg gttggttaag
     8221 ttaccaagga gtagtataaa tttataatgg atttagagta gcactgtaaa tctgttgata
     8281 aaataaatga gcctagatgt aattatatga agaaataaaa tcaactaacg aacaaaatta
     8341 attaatgaaa taaaaaaaca gggaaataaa atacaaataa aaaggtaaat caaaattaaa
     8401 ttaatctgtg tatataaaat tatatattgt gtataagttt tgtatgtgta aacaattaat
     8461 ttaagagata ttatataaat tgtgtattag ggaggaagtt tcgagatgaa gcaatatata
     8521 gtcattgggt gtgggagatt tggaagttca gttgcgtcta ctatgcatct tttaggacat
     8581 caagtaatgg caatagacaa aaatgaagat tcagttcaaa gtatatctga caaggtaacc
     8641 cattcactta tagtggatgt tactgatgag caagcgttaa ggtcattagg tttaggtaac
     8701 tttgatgtag cagtagttgc aataggttct gatataaggg catctataat ggcgactctt
     8761 atagccaaag aaatgggtgt agagttgata atatgtaagg caaaggatga attacaagct
     8821 aaagttcttt ataaaattgg tgcagataga gttgtatttc cagaaagaga tatgggagta
     8881 agagttgcac acaatttagt ttcggataat atattagacc atattgaact tgacccagag
     8941 tattcaattg ttgaaatcgt aactccaaat agttgggttg gcaagacact tatagagctt
     9001 gaattaagag ctagatatga gataactgta cttgctataa aaacaggtaa aaatataaat
     9061 gttacacctt ctccagatga ggaacttaca gccggaagta tcctagttat aatcggtcaa
     9121 aatactagta taacagcgat aacatctgga aataagggga taattagaag aagataattt
     9181 actatttaat atatatttaa ttgcaatgaa agtagatagt atcatataat tatgaatagt
     9241 tatatgatac tattttttta atcgaaggta gtatttattt gtaagattag ataaagagaa
     9301 gttaaattaa aagtaaggag gctgtgctaa tataaaaatt tataagttat tagcatttag
     9361 ataaaatgat tacaaatata aacagtaagg ataatgaaaa gttaaagtat acaagagcac
     9421 tattaaaatc aaagaatagg aataaagagt caaagttcat aatagaggga tacagaatag
     9481 taatgcttgc acttgaatgt atggcaaacc ttgattatgt atttatcaat gaagaatttg
     9541 aaaataagaa agaacatgta aaactattag aagatttgga taaaaaaaac acaaagatat
     9601 acaagactac taataaaaac tttaaagaat tagtggatac agaaaatact caaggaataa
     9661 taggtgtagt ttcatttaag aaaaaaaaat taagtgaaag tataaataaa aaagataaat
     9721 ttgtattgat tttagacaga atacaagacc caggaaatat ggggactata ataaggactg
     9781 ctgattctgc tggagtagat gccataatag cactaaaggg atgtgtcgat atatacaacc
     9841 caaaagtaat taggtctact atgggttcta tttttgatat gaatataatt gatgcttcac
     9901 aagatgaaac tgtggacatg cttaaatcat tggattttaa tatagtttca agttacttaa
     9961 atacagaaaa tttttatgac aaaatagatt atggctcaaa agtagcattg gtgataggaa
    10021 acgaagcaaa tggaataaat gaagaacttg tatcaaagtc tgatattttg gttaagatac
    10081 ctatatatgg taaagccgag tcgttaaatg ctgcgataag ctctgctata ctgatgtatg
    10141 aaataaaaaa atacttaatt taatgtattg taataaatat aatgtcatgt tataatcttg
    10201 aattaaatag tagagtatca aaaatagtca at
//
LOCUS       DAELNY010000135        10117 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.136, whole genome shotgun sequence.
ACCESSION   DAELNY010000135 DAELNY010000000
VERSION     DAELNY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.136"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(17..616)
                     /locus_tag="KPE55_002764"
     CDS             complement(17..616)
                     /locus_tag="KPE55_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG0493812.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(634..3753)
                     /locus_tag="KPE55_002765"
     CDS             complement(634..3753)
                     /locus_tag="KPE55_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0493813.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            3974..4798
                     /gene="pdaA"
                     /locus_tag="KPE55_002766"
     CDS             3974..4798
                     /gene="pdaA"
                     /locus_tag="KPE55_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0493814.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(4895..5680)
                     /locus_tag="KPE55_002767"
     CDS             complement(4895..5680)
                     /locus_tag="KPE55_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0493815.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            6145..6510
                     /locus_tag="KPE55_002768"
     CDS             6145..6510
                     /locus_tag="KPE55_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493816.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            6588..7142
                     /locus_tag="KPE55_002769"
     CDS             6588..7142
                     /locus_tag="KPE55_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0493817.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            7362..8036
                     /locus_tag="KPE55_002770"
     CDS             7362..8036
                     /locus_tag="KPE55_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0493818.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      8391..8487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8541..8717
                     /locus_tag="KPE55_002771"
     CDS             8541..8717
                     /locus_tag="KPE55_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493819.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(8905..9144)
                     /locus_tag="KPE55_002772"
     CDS             complement(8905..9144)
                     /locus_tag="KPE55_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493820.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(9278..10087)
                     /locus_tag="KPE55_002773"
     CDS             complement(9278..10087)
                     /locus_tag="KPE55_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0493821.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         3490 a         1571 c         1006 g         4050 t
ORIGIN      
        1 tataaatatt tttttattat acaccaatag cttttttagc taatttatct gcttcctcat
       61 tatacttatt tccagaatga gctttaacct ttgtaaattt tacattaagc ttattcttaa
      121 tactgtcata gaaattttta tatgctatag ttccttcttt atttgttttc caaactccag
      181 tacaccactt ttcaatccct tcatagtcaa agtataaaat caagttttga acattattgt
      241 caatacagta ctgcattgca attttagaag cctctatctc tccagctaca tttctcatac
      301 ttactaaagt tttctctttt cctttttcac tataagtttt tataacttca ttatttttta
      361 aaatcacaac acccgaacca tacattttta ctgaatgttc ataacttcca tctacatatg
      421 cttctataaa ctccttatct tcttgaaaac tcaatttttc tgacccatca atatattctt
      481 tagcttcttg aaaagtagaa aaactcttat attcagctcc tgagaatcca tttacttgtt
      541 ttttgcactc atcccatgta ttatatacac ctatattttt accttttttc acagcataaa
      601 acttttgttt tgccaaatat atcactccca ctcttaattt ttatcttctt ctctcataaa
      661 tatatctaaa tttataagat agtttctatc aaaatatttt actttcatat ctttttttat
      721 aatatcaata tggtacactt ctccttttac agctctttgt cttatatttt catttatatc
      781 tttttttata atatccttat caacatcatt gttatccatt ttagcatcaa cattcaacgc
      841 ttttatagta tcaaatataa cagattttag tattaaactt ctatatttat ttaaatgtcg
      901 ccttatataa gtataaaact cattgctatt aacctttgaa attttacaat cgtgtaaatc
      961 tttctctaat ctctttaaca cacttatatt agttcctaca tcaaaaatca caaaacatcc
     1021 atagtcatat ttgcttctta aaattctccc catagcttgc tttaccttta tggtcatttg
     1081 aggataattt atagtatagt agtctatacc atacttcttc attatagtaa aatacaaagg
     1141 gtcttttgga tttagattcg gaagcttgtc taaagttaca caaaccaaac catcccctgg
     1201 aatgtctacg ccttcaaaac atccttttga gccaagtact acacattttc tatttaaatc
     1261 gttaaggtgt ctaatcccct ttttatcagc ataaatttct aagttaaaac tatgtaagta
     1321 ttttttcaat atttcataag ttttttcttg tctatcttta ctattaaaaa gagctaatgt
     1381 atgaccttct gtaatctttg atatattaga tataatttta ctcatctcat ttggaaattc
     1441 agaatttctg tactcacaaa tatcagaaaa tccaactaca gagactctat ttttataatc
     1501 aaaaattggt tctataatct tttccacatt cttaactctg tctattccta atgtattttt
     1561 aaaataagac atactttcag ataaactcaa agttgctgat aaaaatattc ctttttctaa
     1621 ttgagataaa atattttctt caaataaatc tgctattttt aatggcacta ctcgaaattc
     1681 aaagtcatta taattcttat caatttcaac tatccttgca taatcatcct tttcatcata
     1741 ctctaaaaag atttcaaata taatctttat atcctctaaa tctcttgtct ttgcctttcc
     1801 aaatttatat atatctgctt ctttatctat actatcatca tcaatattcc tataaattat
     1861 tattattatt gaaactaaat tttttattat tttctcacaa ctcaacttta tcttttcact
     1921 ataggctcta taacttattt cagtatctat accatccttt tttagttttc ccacaatttc
     1981 gtctatttgc aaattcagtt cccatcttaa attataatta cttacattat tatattcatt
     2041 acaattacca aactttaata ttgaatctat ctcttcaata atgagattta tacttctagc
     2101 aattttttgt ttatcttctc ttccaatttt taatacatta taaaatttat caaaagcctt
     2161 tatttttctc atatttctac tatatttttt atatataaag ctactatttt gaataaactc
     2221 gtaaggataa atttcttgta ataaatacct taaagacttt gaatttataa tgcttgaaaa
     2281 gaaatcatat cctttttcag tcaaattatg tgcttcatct actattatat tttctaaagg
     2341 tttttcatcc ttatatggcc atttagctaa tagtgaatga tttactactg ttatatgttc
     2401 ttcttttaat tcttctattc tgtttttata taaacaatct tttttgcatt ttttaggtct
     2461 gcacatattt gggtcacaac ttacatttct taagtgagta cttatttcct tgaagttgtc
     2521 aaacactaaa taatttattt cttctatatc tccatacttg ccaccttcaa ctaatctctt
     2581 caaaaatatt aaagataaaa tttctccttt agtagggttt tgagactcat aatcatctat
     2641 atatgcttcc agtctatcta tacaaatata gttatttttt ccctttatat atccgtaact
     2701 tacttttcca tttagaccca aagaatctaa cacatttgga atatctttat ttattaattg
     2761 tatctgcaat tcctttgtgt cagtagaaat taataatctt tttttattaa ttctagcctc
     2821 taatattgca ggcaataaat atcctacact tttacctata ccagttggtg cctctataca
     2881 tgcaattttt tcttcatctt cgctatttct aaacaactct cttattgttt tagtaagctc
     2941 atattgacca ggtctatatt catatgtaaa tccttcttta ttctcccaaa tagttttgta
     3001 ttttaataat tcttcataat ttttttcttc ttcatttaaa atttttaagg tttctctttc
     3061 cttcttcttt tcttcttttg agtcaaatat attatattct ttttttatat ttatattatt
     3121 tgataaatca taatttgcct cttctaaaaa tttgctccat tcccatgttg gaagattaaa
     3181 ttttttaaga tatgaattta ttttaaaact caatggctcc aaagtagttt tttcatagtt
     3241 attaaatttt actaaaaggc aatttacaat atttattata tcaatagcat ctgataaagc
     3301 tctattttcc actttagaat tacaatttgt taaagtattt tttaagtatt ctaagctata
     3361 atctttatga tatggttcta aaataactgc taattcaatt aaatcaataa atttgttttt
     3421 taactttggc atatgatact caagaaactt ttttttcaga cttaaatcat gacatataat
     3481 gaaactatct tctacaaaat ttacaagtct atcttccacc agttctattt cattagctat
     3541 ttctaaatca tttttatcta aatttttaca tactgagaat acttctacag gtacctcaaa
     3601 tttgtttttt attaatgatt cataggtata tattttccag tctttaacct tgacagctcc
     3661 aacttctaaa atttctgaat ttaaacaatc taatccacta acttctatat ctaaaaaaac
     3721 aacatcattc aatacactta ttatattatc catttttata tcccttcaag ttaaaccagc
     3781 ctaacttatt tacaatcatt tctctttata ttttatcatt tatatacact ttatttaaga
     3841 taattaatat caactttata tcttacagta aacttctttc atttattatt tgtatatgtg
     3901 aatattttta actataaata ttcaaactat attataagaa atatacttta aattttaaat
     3961 aagaggtgaa attgtgaaaa aagatagcct aaaaaaatac attatgatag gagcatttgc
     4021 tctaatactt tttggaattg ctagtataaa ttttaaatca ttagataaaa caaaaactca
     4081 aatttctagt ccaacactcg acacacatga atatgattgg tattttaacc ctagggagga
     4141 tggtaaacag ccatcaccta taaaagaagc tgattttttt aaaaaatacg gagcttatta
     4201 tgtaggaaat cccaatgaaa aagttatata tctatcattt gatgctggat atgaaagtgg
     4261 aaacactcca aaattattag atacgcttaa aaaacacaat gctaaagctc aatttttcgt
     4321 agttgaaagt tatataaaaa gcaatccaga gttaataaag cgtatggaaa aagaaggaca
     4381 tttagtttgt aatcattcaa aaagccatcc atcaatggca ggtattacag attttgaaaa
     4441 attcaaagaa gaaattacaa gtgtagaaaa agcatacaaa gatgttactg gaaaagaaat
     4501 gcctaagtat tttagacctc caatgggtaa atttagcgaa caatctttaa aatatacaca
     4561 agatttagga tataaatcaa tattttggag ttttgcttat gtagattggt atgaaaagaa
     4621 acaacctact catgaatttg ccaaaaacaa aatttattca agaactcatc ctggtgcaat
     4681 agttttactg catccaaatt catcaacaaa tactgagata ttagatgagg ttcttacaca
     4741 ttgggaaaaa gaaggttata aacttaaaac acttgattat ttaaataata aaaaatagta
     4801 tcataaattt aaaaaaactc tatcatgaat tatagtaaaa atatagttat ttataaatac
     4861 tttaattctc gatagagttt tttatttttt atagctattt aaaaagaatt gtccattcat
     4921 ttatctcttt gtatcctata ttatgatata ttttacctgc tacatcatta ttataaaaaa
     4981 gacatggaac ttttccttct aacaacaatt ctttactcaa attgtaaacc atataacttg
     5041 caagtccttt tcccctatat cttttatctg tacttacaga gaccaccatt gccaaaaaac
     5101 tagtttccat accagttgac acagtgctaa tcatagtatc atcatttcta ataaaatatg
     5161 ctcttacatt tccctctttt aaatgaactt ttctttcttc tatgtaattt ttacttactt
     5221 tatagtctga cctatttaaa agttttccta tttcatcaat atctttttcc tgagcatttt
     5281 ctattttata ctccttcatg ttggaaaaat ctatttcttt tatttctttt aatacacaaa
     5341 atttattatc tagtttttca taaaaatctt tgtatttact tattagtcta tctattacac
     5401 atttttttcc acttatgctt tctacatcta aatcttttat atgattcttc atttctccta
     5461 tattaaaatt atttttaaaa ctataaatta taagtgttga cttatttttt aatattatag
     5521 aggttatatt tccatcttct acctgagacc atatatcttg atattgagag ctaaatccat
     5581 aattctcaac gtatccaatt atgaataaat ttaaactttc ttcatgtgat acatattgcc
     5641 aaaattcttt ttctaaagta ttatctattt ttacaatcaa gcagatatcc tccttctatg
     5701 ttaataatga tataccttat aatttatagt atcatttaaa atcattatat tctaccaaat
     5761 attctaaatt tttaaatatc tatgcaatat gttcgtattt atttttatat tctgttactg
     5821 cttcactcca agcttcttct actgaaatac ctctcttatt tgcaattttt tgtgctaatt
     5881 ctattatttt catagctttt cttgatatat tcatatatat tcctcctcta aattatcata
     5941 agttatttta aagttaaatt tctatagtca tgttatatat ttatttactt atatcttact
     6001 aattatttat gtactaaata tgaactacaa tagaagaaat ttagaactta ataaaacata
     6061 aaatttatat acagttcata tatatttgtc atatcacaag acaagtgata taatttatta
     6121 tcattttact gggggtgata tgctatgaaa ataagagctg aatatacatg tcttcttgaa
     6181 attgtacatg acataatcaa aggaaaatgg aaaactatcc ttatatttga attaagacaa
     6241 tctaataaaa cattttctga attgcaacat aatatttatg gaataaatca aaaaatgttg
     6301 attcaacaat taaaagagtt acgtgatttt gggattattg ataaaataag ttctgctgga
     6361 tatccattac atgttgaata ttttcttact aatcgtggta aaaaaatgtt aaaagcagtg
     6421 gaaataatgc aagaaatagg aatcgaatat atgcttgaac atggtcaaca agaaattcta
     6481 gataataaag gaatttgcta caataagtaa tcctatacat accaaaaagt aagtaattta
     6541 cattttttac aatagtatta taataaaaat aaataggagg tataatcatg tctatagctt
     6601 taatattgat tgatattcaa aatgattatt ttaaaaatgg aaaatgtgaa ttatttcaat
     6661 cagaagaaac tgctgaaaat gctaaaaaaa tactactgtt ttttagaaaa aacaatttac
     6721 cagttattca tatacagcat atatcaacag ataaaacagc ttcttttttt cttcctaata
     6781 ctcatggttc agaaataaat aaaattgttt ttccactaga taatgaagaa attataacta
     6841 aacacacacc agatagtttc tttgaaacag atttgcaaag tatccttgag aaaaaaaata
     6901 ttactaatct agtaatctgt ggaatgatga gccatatgtg tattgataca agtgtaagaa
     6961 cagcaaagaa acttcgatac gacataactc taataagcga tgcatgtaca acgaagaatt
     7021 tatcttggaa taatatagaa attaatgcaa atactgtaca tcaagtattc atggcatcac
     7081 tacagaacag ttttgcagat gtaaaaaaca cagataaatt tttatctaac tatcacaaat
     7141 aaaaacatac actatgttaa aagagcaagt ttgatttatt taagcttgct ctttaatcat
     7201 ttaaaattag atgctttcta tcatttttat atacataata tacgtttata cattttatat
     7261 tcatatataa ctattatata aattttaaac tttactatat tcacataaaa aatgtagtat
     7321 tatagtatat ataatattat atagaagggg gtgctagata aatgaaccat catatattga
     7381 ttcaatttga atatatagca agaatcatta ctgctggttt ctgtggggct ttaattggat
     7441 atgagagaaa gaatagacta aaagaagctg gaatacgcac tcattttata gtggcattag
     7501 cttcagcact tataatcatt gtttccaagt atggatttaa tgatatagtg ggtactccag
     7561 gtattggtct agacccagcc cgcgttgcag cacaagttgt cagtggtata ggttttttag
     7621 gtgctggatt aatatttatt agaaatcaat cagtaagtgg tcttactaca gctgctggta
     7681 tatgggcaac agctggtaca ggtcttgcca ttggctctgg attatactta gttggtattg
     7741 tttcaagtat cattattgtt attgtacaga taacacttca tagagatagg aaatggatga
     7801 aactccctac agcagagcaa gtttctctta aaatttctaa cagcactgat tccatagaat
     7861 atattgaagg taaatttaaa aaatctaata ttgaaattat aaacatgaat attaaacgcc
     7921 ttgatgggga ttggttagaa gtagttatat atactaagtt acctaaagga tataaaaaaa
     7981 caaatttatt aaaattattt agcgataatc ctcttataga aactttagaa atataatata
     8041 aaaattgaag cttaaaaata ataatatatt taatcaaggt caatatataa ataattacgg
     8101 gatgagtcta tataaaattc atcccttgtt ttttgccatt catatatatt ttttcaagat
     8161 atttttgttc atctattatc tagccaagta actcttgtga actaaacttt ttaatacaaa
     8221 aacttcaata caaaactaaa tataattttt attaaatatt ctacatgtat agtttatttc
     8281 ttgagtgaaa aatagatttc atattgttaa gatactgtta aatatttcaa ttttctgaca
     8341 agtatgttga acaataactt atcctagtgt attattttag ttgtagaata aaactaaatc
     8401 ggcaaaacta gagaaattta gtgacgcaaa gctataggga ctaacgctta taaaataaac
     8461 tatgagttat gtcagccagt tgccaaaaag atattgttct ttttgtttta tgaaagtttt
     8521 ttagggggat agtaatatat atgtttagag atgaaatgga taaatgtaca cacatgttaa
     8581 ctgcttatat cagtagttta tatgattatt gtgattttat agatacacag ctagatgatt
     8641 ttatactaga gtacggagaa aatgtagtag aatcttgttt acatcaagtg atggtattgg
     8701 taagtaagta taattaaata gaaattgttt tctagagaac cctttttagt tttatctaca
     8761 tttaaaaatg ttattatatt aaaaaactaa tctcccctat atgcaaagtt ttcttccata
     8821 taacaatttc ttcccattta tactttttca cttgtatctt taataaaatt caacttcaaa
     8881 tattaatata cttcaaaaat tattctattt cttatcttta caccaattcc tatcagtatt
     8941 atcctttata taggttctaa ctatttctcc acaatctaaa cataaatctg aatatatagt
     9001 agattgtact ggaaaagttt tatttataac tttatctaat tttccatctc ctctatattt
     9061 taatccaata gtttcagcat atatgccacc agttgttaaa attccctttt caatatttac
     9121 acttccacaa ttagaacatt tcacatttat aaccccctaa acaattattt tttcatgcta
     9181 tcttataatt taaaatataa tttgctaccg attttataac acagtggcaa atcaacattt
     9241 tttcattcta tttaccatct aatccattga aatatagcta tttagttata ggaacttgaa
     9301 tttccgttac aaactcctct acaattccag tctcatgtat atctatctta tagatttcta
     9361 tagggtcagc aattatctta taattatttt cttctataaa tttaaacacc ttctccatat
     9421 atatcttatt attagaatta ctcccagtat aatttaaagt tacataatat ccttcatcaa
     9481 aaactatatt atatactttc tcattacttt ccagaaaaca aaatacagat ttaaactcat
     9541 taaatatacc tttattaatt gcttcagtag aaaaggcaga accaatattg ttgtttccaa
     9601 gtatattaaa tctatcttca tattctttct gaagtttttg aattaaaaaa tcgaagtctt
     9661 catccctctt tatatcagca tttaattcta aagcttttct tttttctata tatacaacct
     9721 ctatctcatc tatttttgta ttttgaatta cactttcaat tgaactcaat ctttttataa
     9781 tattttcctt atggcttata aattcagcta tttttttatc aattaaatcg agttcttcat
     9841 ttaaaatatt tttcgtagac tctatacttc tgtcatctag gtattctttt attttcttca
     9901 ttggaaagtt caagcttcta aattctttta ttaaatttaa tttccatata tctgatatat
     9961 tatacattct atatccattt atatctctaa ctggtcttag aatgcctatt tcttcataat
    10021 acattaatga atctctacct attccatata ttttagatat ctctcctatt ttatagtaat
    10081 ctttcattta tataataaac tcctccgaaa tcttatt
//
LOCUS       DAELNY010000136         9952 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.138, whole genome shotgun sequence.
ACCESSION   DAELNY010000136 DAELNY010000000
VERSION     DAELNY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.138"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1004
                     /locus_tag="KPE55_002774"
     CDS             <1..1004
                     /locus_tag="KPE55_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0493822.1"
                     /translation="DKGYYFNSNWKDIHIGIYDTKLGDSLKYITNEYIENMERVKYDY
                     RELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKALEYVKEQTEYLC
                     LEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQTRRICLEAIKKDGR
                     CLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMALQYVNKQTDKICI
                     EAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTEDICIEIVRQNGK
                     MLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKEYVKIAIDDCI"
     gene            1143..1595
                     /gene="tsaE"
                     /locus_tag="KPE55_002775"
     CDS             1143..1595
                     /gene="tsaE"
                     /locus_tag="KPE55_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG0493823.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1595..2311
                     /gene="tsaB"
                     /locus_tag="KPE55_002776"
     CDS             1595..2311
                     /gene="tsaB"
                     /locus_tag="KPE55_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG0493824.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2301..2777
                     /gene="rimI"
                     /locus_tag="KPE55_002777"
     CDS             2301..2777
                     /gene="rimI"
                     /locus_tag="KPE55_002777"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG0493825.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2783..3799
                     /gene="tsaD"
                     /locus_tag="KPE55_002778"
     CDS             2783..3799
                     /gene="tsaD"
                     /locus_tag="KPE55_002778"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG0493826.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4096..6804
                     /gene="hpdB"
                     /locus_tag="KPE55_002779"
     CDS             4096..6804
                     /gene="hpdB"
                     /locus_tag="KPE55_002779"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG0493827.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVQILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6808..7065
                     /gene="hpdC"
                     /locus_tag="KPE55_002780"
     CDS             6808..7065
                     /gene="hpdC"
                     /locus_tag="KPE55_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG0493828.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7058..8008
                     /gene="hpdA"
                     /locus_tag="KPE55_002781"
     CDS             7058..8008
                     /gene="hpdA"
                     /locus_tag="KPE55_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG0493829.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8323..9285
                     /locus_tag="KPE55_002782"
     CDS             8323..9285
                     /locus_tag="KPE55_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0493830.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9384..>9952)
                     /locus_tag="KPE55_002783"
     CDS             complement(9384..>9952)
                     /locus_tag="KPE55_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0493831.1"
                     /translation="YPVKNIKLGSLLIIYFMISVIVPTSFILILQGAQPNYSGIIKFI
                     FTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFTLYT
                     PEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKESTITY
                     HDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         3893 a         1045 c         1856 g         3158 t
ORIGIN      
        1 atgataaagg gtattatttt aatagtaatt ggaaagatat tcatattggt atttatgata
       61 caaaattagg agattcttta aagtatatca ctaatgaata tattgaaaat atggaaaggg
      121 taaaatacga ttatcgtgaa ttaaaaaata tagaaaatca aactgatggt atgtgtattt
      181 tagcaataag gcagaattac aaggcattag agtatgtaaa aaatcaaacc gaagaattat
      241 gtttagaagc aataaagtat aattataaag cacttgaata tgtaaaagaa caaacagaat
      301 acttgtgtct tgaagctata aaaaaagatt gtaatgcact taagtatgta cgaaataaaa
      361 cagaagggct tattataaaa gcaataagtc attcttccaa tatagatgtt gtaagtatac
      421 taaaggcttt agagactcaa acaagaagga tatgtttaga agcaattaaa aaagatggca
      481 ggtgtcttgc atacgttaga gaacagagtg aagaattatg tatagaggcc attaagcaaa
      541 attataaagc attaaaatat gtaaaaaatc aaacggaaaa aatgtgtata gaatccgtaa
      601 gacaaaatgg tatggcatta caatatgtaa ataaacaaac tgataaaata tgtatagaag
      661 ctgtcaaaca agatggaaga agtctacaat ttgtcaacaa taagacagaa gaaatatgta
      721 taaatgctat aagatattta aataaaaaat ataatattaa agatgtacta agttacatag
      781 ataagtatac agaagatata tgtatagaga ttgtaagaca aaatggaaaa atgcttatgt
      841 atataaaaaa tcaaacagaa aaaatgtgta ttgaagcagt aaaagagaac tataagtctc
      901 ttaagtatgt aaaagagcaa tctgagagaa tatgtaaaga agcattgaag caaaaccata
      961 aagcaaaaga gtatgtaaaa attgctattg atgattgtat ataaggtgta gatacagcca
     1021 tcaatagtga aagataaaaa aagataaaga caagtacttt ataataatct aggcttatac
     1081 tataattaat taagcagtga tataaattca catgacgatt atatcaaaat ggagatgaat
     1141 tgatggcaaa aatatattta gaaaatgaaa ataaaactag agaaatagga tataaattag
     1201 gtaaactttt aaaagaaggc agtgttatct gtttagtagg agatttagga gctggtaaaa
     1261 ctacaatgac acaaagcttg gcagattcat tagggataga agattatata acaagtccta
     1321 cttttactat aataaatgaa tatgaaggaa aaataccttt gtatcatttt gatgtttata
     1381 gaataggtag tagtgatgaa atgtatgata ttggttatga tgaatatgta aattcaaatg
     1441 gaatctgtat aatagaatgg gctaatctaa tagaagatat acttccaaaa gaatatttga
     1501 atatagaatt gagatataaa gatgaaggaa gagaaatgat attaactcca aaaggagagt
     1561 tttataaaga gatagttgag gagttgatta aataatgaaa atactaggta tggatacgtc
     1621 ttctatggca gcaagtgtag cagttgttga agatgataac ttaatatgtg agtttacagt
     1681 aaacaacaaa aaaactcatt cacaaaagct tatgccaatg atagaaaata tgttgagtat
     1741 gagtgattta agtataaaag atatggattt attagctgta tgtataggtc caggttcgtt
     1801 tacaggactt agaataggta tggctacagt aaaagctatg gcccatgtaa ataatatacc
     1861 aataatagca gtaaattctt tggaatctct agcaaataac ataaatttct gtgatagaaa
     1921 gatatgttgt atattagatg cgcagaaaaa tcaagtttat tcagctaaat acaagtttga
     1981 aaatggagag atgatagaat tagatggtgt tgatgttgta gagtttgatg ctttagtaga
     2041 tgaaattgta tcaactaatg aagaatttat aatagttgga gaagctgtat acaaatataa
     2101 agacaagtta gagaatataa aaaatataaa aataccttca ccagctaata atgtatctaa
     2161 cgcaggaagc ttatgctctt tagcattaaa taaatataat aaaaatatag atgtacatac
     2221 ttgttatacg ataaatccta tgtatatcag aaaatctcaa gctgaagtac aatatgatga
     2281 aaaaatgaag aggctaaata atggaagata atacaaaact aatagtagat gatataaaaa
     2341 tagaagaaat gactacagaa gatatagatg aagtttttga agtagaaaaa aattgttttg
     2401 aagattattg gtcaaaagag tcttttagaa aagagttgtc taatgaagta gcaaggtata
     2461 tagtagctaa gttaaatgga aaaattgtcg gatatgtggg aatttggctt atacttgatg
     2521 aagggcatat aaataatgtg gctgttcata gtgactatag agggaaaaaa ataggggaca
     2581 agttgataaa aggcatagtt gatttatgta aagataataa tattgcatct atgacattag
     2641 aagttagagc atcaaataaa atagcacaaa atttatatag aaaatatgga tttaaaatgg
     2701 gtggaataag aaaagaatac tacagtgata ataaagaaga tgcaattata atgtggaacc
     2761 aattaaaaga ggtgtagagt ttatgagtga tataataaca ttagctatag agagtagttg
     2821 tgacgaaaca gctgcatcag ttttaaaaaa tggtagagaa gtactttcaa acataatctc
     2881 tactcaaatc gagacacata aaaaatttgg tggtgtagtt ccagaagtgg cctcaagaaa
     2941 acatgtggaa aatattgata tagttgttca agaagcttta gacaaagcaa atataggttt
     3001 taatgatata gaccatatcg cagttacata tggacctggg ctagtaggtg ctttattggt
     3061 tggtctatct tatgctaaag cacttgcata tactttgaat attccacttg taggagtaaa
     3121 tcatatagaa gggcatctaa gtgcaaacta tatagagcat aaggatttaa aacctccttt
     3181 tataacatta atagtatctg gaggtcatac tcacttggtt gaagtaaaag attatggcaa
     3241 gtatgaaatt ttaggaaaaa caagagatga tgcatctgga gaagcatttg ataagatttc
     3301 aagagctatg aatttaggct atccaggagg tcctattatt gataatctgg ccaagaatgg
     3361 aaataaacat gctatagaat ttccaagagc ctatttagaa gaagatagct atgattttag
     3421 ttttagtgga ttaaaatcct ctgtattaaa ttatctaaat ggaaaaagaa tgaaaaatga
     3481 agaaatagta gttgaagatg tagcagcatc tttccaagag gctgtagtag aggttttatc
     3541 tactaaagca ctaaaagcag taaaagacaa aggatacaat atcataactc tttctggtgg
     3601 ggttgcttct aattcggggc ttagagctaa aataacagag cttgctaaag acaatggtat
     3661 tactgttaag tatcctcctc taattttatg cacagataat gcagctatga tagggtgtgc
     3721 aggatattat aattttataa atgggaaaac gcatgatatg agtttaaatg cagttccaaa
     3781 tttaaagata aatcagtaga gttagatgaa tttgtaaata taaaagtatt gtttcaaaaa
     3841 agtaagtaga agtgaaatta aatataataa aaataaatat agtatatatt aaaacttgaa
     3901 gtaaaactat ttataatctt tatattccaa tgaattgcaa caaattttag gatatacaga
     3961 ttgtctttgg cttagatttg cttcaagttt aattttttct atagaaagat tattttaaaa
     4021 agtgggtaat ataccctttt ttttcattct taaaaagttt tatactaatt atagaaagat
     4081 taaggagggg aagaaatgag tcaaagtaaa gaagacaaaa taagaagtat tttggaagca
     4141 aaaaatatta aatcaaattt tcaaaataaa gaaaatttaa gtgaatttaa tgaaaaaaaa
     4201 gcaagtaaaa gagcagaaga cttattagat gtatattata atacattatc tactgctgat
     4261 atggaatttc catattggta taatagagaa tatagaaaat cagatggtga tataccagta
     4321 gttagaagag ctaaagctct taaagctgct ttttcacata tgacaccaaa tataatacct
     4381 ggtgagaaaa tagtaatgca aaagacacgc cattatagag gttcttttcc aatgccatgg
     4441 gtaagtgaaa gtttctttgt tgctcaagga gaacaaatga gagaagaggc aaagaaatta
     4501 gccagtaaca cagctgatga acttacaaaa tttgggtctg gtggaggaaa tgttactgaa
     4561 agttttggaa atgtcgtttc aatagctggt aaattcggaa tgagaaaaga agaagtacct
     4621 gtacttgtaa aaatggcaaa ggaatgggtt ggaaagtctg ttgaagattt aggttttcac
     4681 tatgaaaaaa tgatgccaga ttatgattta aaagaaaacc taatgagtac acttatatgc
     4741 atgtttgatt cagggtatac acttcctcaa ggtagagaag ttataaatta tttttatcct
     4801 ttaaattatg gattagatgg aattatagaa atggcaaaag aatgtaaaaa ggcagttgca
     4861 ggaaatgcat ctggagatgg gcttattggt atggaccgtc tatattttta tgaggctgtt
     4921 attcaagtta ttgaaggatt acaaacttgg attttaaatt atgcaaaaca tgcaaaatat
     4981 ttagaaagta tagagacaga tttagaagct aaaaaagaat actcagattt agtgcagata
     5041 ttagaacata ttgcacataa acaacctcgt acatttaggg aagctcttca gttgacttat
     5101 acaattcata tagcatcagt aaatgaggat gctatatctg gtatgtctat aggacgtttt
     5161 ggtcaaattt tatatccttg gtatgaacaa gatatagaaa aaggactaat tacaaaagaa
     5221 gaggttatcg aattattaga gctttataga ataaaaatca cttgtattga ctgttttgct
     5281 tcagcgggcg taaatggagg agtactatca ggcaatacat ttaatacttt atctataggt
     5341 ggattaaaag aagatggttc tacaggagct aatgaactag aagagttatt attagaagca
     5401 agtatgagat gtagaactcc tcaacctagt ttaactatgc tatatgatga aaagcttcca
     5461 gaagatttct taatgaaagc tgctgaatgt acaaagcttg gttctggtta tccagcatgg
     5521 gtaaacaact caaatggaac aacatttatg atgaaacaat ttgctgatga aggtatgact
     5581 gttgaagaag caagagcatt tgcattaggt ggatgtttgg aaacgtctcc agggtgttgg
     5641 aaacagttaa ctttaaacgg aaaaacatac tctattgcag gtggagcagg tcaatctgct
     5701 ggctctggag ttcactttat agctaatcca aaaattttag aacttgttct tatgaatggt
     5761 aaagactatc gtatgaatat acaagtattt gagcctcata ataagccatt agatacttat
     5821 gaagaagtca tagaagtatt taaagattat tataaacaag caataaatgt tttagaaaga
     5881 gcaaataaca ttgagttaga tatatggaga aaatttgata cttctataat aaattcacta
     5941 ctaaagccag actgtttaga caaaggtcaa catataggaa atatgggata tcgttataat
     6001 gcaacattaa acgtagaaac ttgtggtact gttactatgg tcaattcatt tgcagctctt
     6061 aagaaacttg tatatgatga caaagcattt acaatagagg aaatgaagga tgccatatta
     6121 aataattttg gatttaaaga tgcattagaa gtaggaaatt attctatggc ggaccaagta
     6181 aaagtagata agactggtaa atatgatgct atttataagg cctgtcttga tgcacctaaa
     6241 tatggaaata atgatttgta tgcagataat attcttaaaa actatgaggt atggttatca
     6301 aaagtatgtg aagaagcaca atctttatat gctaagaaga tgtacccttg ccaaatatct
     6361 gtttctacac atgggccaca gggagctgct accttggcta ctccagatgg tagactaagt
     6421 ggaacaacat attcagatgg ttcagtctct gcatatgcag gaacagataa aaatggagta
     6481 tatgcattat ttgaatctgc aactatttgg gaccaagcag tagttcaaaa ttctcagatg
     6541 aatttaaaac ttcatccaac tactataaaa ggacaacaag gaactaaaaa attattagat
     6601 ttaacaagaa gttatttaag aaaaggtggg ttccatattc aatataatgt tgtagattca
     6661 gaaacattaa aagatgcaca aaaaaatcct gacaattatc gtcaattaat ggtacgtgtt
     6721 gctgggttta ctcaatattg gtgtgaatta ggtaaaccaa tacaagatga agtaattgct
     6781 agaacagagt atgaaggggt gtaaaatatg agaaagcata gtgattgtat gaatttttgt
     6841 gcagtagatg caaccaaagg aatttgtaga ttatcaaaac aaatgattaa tttagatgat
     6901 gcagcatgtc cagagataaa agtaatgcca aaatgtaaaa attgtaaaaa ttttgttgaa
     6961 gctaatgatg aaggtatagg caaatgtgtt ggcctagaga aagaagactg ggtatattca
     7021 acattgaatg caattacttg tgaaggacat gtgtttaatg agtagtcaaa agcaattaga
     7081 aggcatgatt tttgatgtac aaagcttttc agttcatgat ggtccaggtt gtagaacaac
     7141 tgtattttta aatggatgtc cactgagttg taaatggtgt gcaaatccag aaagttggac
     7201 tgtaagacca catatgatgt ttagtgaatt atcttgtcaa tatgaaaatg gatgtactgt
     7261 atgtcatggt aagtgtaaaa atggtgcatt gagctttaat cttgataata agccagttat
     7321 agattggaac atctgtaagg actgtgaaag ttttgaatgc gtcaactcat gttattataa
     7381 tgcgtttaaa ttatgtgcaa aaccttacac tgtagatgaa cttgtgcagg taattaaacg
     7441 tgattctaac aattggagaa gtaatggagg agtaacattt agtggaggtg agccactact
     7501 tcagcatgaa tttttacatg aagtattatt aaagtgtcat gaggtaaata tacacactgc
     7561 cattgaaacg agtgcatgtg tatcaaatga agtttttaat aaaatattta acgatattga
     7621 ttttgcattt attgatataa agcatatgga tagagaaaaa cacaaagaac aaacaggtgt
     7681 gtataatgat ttaatattag aaaatatatc aaaccttgca aattctgatt ggaatggaag
     7741 attagtactt agagtaccag ttatctctgg gtttaatgat agtgatgaaa atataagtga
     7801 tattatatct tttatgcaca aaaataattt ggtagaaatt aatctattac catttcacag
     7861 attaggagaa tcaaaatgga ttcaattagg caaggaatat gagtattctg ataagggtga
     7921 tgttgatgaa ggtcatctag aagaattaca agatatattt ttggataatg gtatagcttg
     7981 ttatgttggc catgagacag ctttctaaaa ttaaatacaa gttttaatta aaaaaggatg
     8041 ttacactaaa agctagagtt acatcctttt ttatgtggtt tttatgtaga aataattaag
     8101 taaagtttta tatgttttat atattgttta tattgcttta tgtccataaa tattgcttta
     8161 aatatacttt atatattttt tatagtttaa attacagtta gagaatatat tacgtttact
     8221 tcattgaagc agaagttttg tggtaaaata ctactatgaa aaaatttaat agaatatatt
     8281 ttagaaacaa ctaaaaataa tagttaggag gaagacatct atatgaacaa atcatggagc
     8341 aacttgatta ttaagaataa agacagaaaa gtacgttcag ggtggattat tatagctgtt
     8401 atggctacct tttacatact tcagtattat ttaagcatga tacttgtgga aataataaga
     8461 aaaatcctgg ttgctacagg tgacattaat ttaaaaacaa actatttttc agaatatgct
     8521 aattggttaa atgatgttgg gttacagatt gtatttcaaa ttctaacaga ttcacttatg
     8581 attattattc ctataattgt atggagattt attatgaaaa atcctattag tgaaatggga
     8641 ttacattctt atagaattaa gaaaaaagaa ggatatgtag gaatgttatt aggaatattt
     8701 aattgtacag taatctttat tataacaata acaattggtg gtggtcatgt tacttcatgg
     8761 gtaccaaaaa tttcaccttt aacagtttct tggatactgg tctttatttt agttgctttt
     8821 gcagaagaaa ttttaaatcg tggttttttt atggcagttt tacgaagatg taaaaatatt
     8881 tattttataa tgattgttcc atctgttatt tttgggttga ttcatatatg gaatcctgat
     8941 gttacttttt tgtctgtaat taatattata attattggga ttcttttttc atatatgttt
     9001 ataaaatcgt cgaatatatg gatgtgtata ggttatcatt ttgcatggaa tgtatttcag
     9061 gggattatat atggtatgcc tgtaagtgga cttcaagttc cagggattat aactacacaa
     9121 atcactcatg gtaatctatt aaatggagga atgtttggta ttgaaggtgg aattttgact
     9181 acttttgtta atctacttag ctttatattt gtatggtatt actatagaga ttctaagtat
     9241 gattttgtta gtgataaagt aaatgtgacc aatatcaaga attagttgat tataataaat
     9301 tttaataata tcttctaagc ttaaagtatt ctgtaggatt gttggaaatt tgcaaaaata
     9361 taaaaataac ttggatattc tatttactcc aagttatttt tatattcttt tgtaaataga
     9421 aatggaacat aaaaaattaa aacaaccata gatactatta gtatatcatg gtaagtgata
     9481 gttgattctt tagaatttgt gataagaaaa gcactattat ttaaaacatg aattatagca
     9541 caaaaccaga tattcttagt ttttatataa agatatccta aaaatattga aattccaact
     9601 atatgtattg aacgtaatat tattcctaat attggcgctt caggtgtata taatgtaaat
     9661 attagtggta agtgccacaa gctccaacac atacctaata ttataactcc taacttttta
     9721 ccaaatttat caaaaaaaat attctgtaaa aaacctctcc aggcatattc ttcactaaaa
     9781 aaaatataaa tagtagttaa tgatgttatt ggagtaaata taaattttat gataccacta
     9841 taattaggtt gagccccttg tagtattagt ataaatgatg ttggaacaat tacagaaatc
     9901 ataaagtaaa taattaataa agaacctagt ttaatatttt ttacaggata ta
//
LOCUS       DAELNY010000137         9843 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.139, whole genome shotgun sequence.
ACCESSION   DAELNY010000137 DAELNY010000000
VERSION     DAELNY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.139"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1775
                     /locus_tag="KPE55_002784"
     CDS             <1..1775
                     /locus_tag="KPE55_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0493832.1"
                     /translation="EDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRING
                     YYIVGNEMEIRRVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYE
                     ITFVEDRLIEFIYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYG
                     SEDKIKPHNIMYISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQE
                     KIFKQLYSHFRPAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQ
                     EEELAYLTMHFATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIP
                     MSESVGLENISEPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGD
                     IFLRQPKIDEVMDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNET
                     LVKIKVKAKNWEEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHA
                     RPEAGSKEIAIGIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEED
                     EFFKVLDSAKEAKEIIDFIKTHEL"
     gene            1790..2077
                     /locus_tag="KPE55_002785"
     CDS             1790..2077
                     /locus_tag="KPE55_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493833.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2104..3375
                     /locus_tag="KPE55_002786"
     CDS             2104..3375
                     /locus_tag="KPE55_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG0493834.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            3433..4032
                     /locus_tag="KPE55_002787"
     CDS             3433..4032
                     /locus_tag="KPE55_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0493835.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4037..4942
                     /gene="dapA"
                     /locus_tag="KPE55_002788"
     CDS             4037..4942
                     /gene="dapA"
                     /locus_tag="KPE55_002788"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0493836.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5048..5680
                     /locus_tag="KPE55_002789"
     CDS             5048..5680
                     /locus_tag="KPE55_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG0493837.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVKKI"
     gene            6158..8665
                     /locus_tag="KPE55_002790"
     CDS             6158..8665
                     /locus_tag="KPE55_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0493838.1"
                     /translation="MKEDIGLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDTYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNIVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSMI"
     gene            9007..>9843
                     /locus_tag="KPE55_002791"
     CDS             9007..>9843
                     /locus_tag="KPE55_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493839.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKRLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILKELNVDRSIFIARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICI"
BASE COUNT         3736 a         1098 c         1764 g         3245 t
ORIGIN      
        1 tggaggatat ttcaattaat cattttataa attctataaa agtaagtagg tctactgtaa
       61 atcttgattt caaggatttg ataccaaagc ttgaaaagaa gaacattaaa gttaagaaca
      121 atcgtataaa tggatattac atagttggca atgagatgga aattaggaga gtgcttatta
      181 aaaatattat agaaactttg tctaatgaga aaagttcaag ggtatttgat atttttatta
      241 aagaatataa tttagatagt tttgaagagg ctaaaaaaat tatattaaag ctaataaaaa
      301 aatatgaaat tacttttgtt gaagatagat taatagaatt tatatatata tttatctttc
      361 taaaggcaag gatgtattca actagaatag ttactcagga aaatgtagat attcctaata
      421 tttcagttat gagttcaatg aaggagtata aatttgctaa ggaattatta gaagtatatg
      481 ggagtgaaga taaaataaag cctcacaata taatgtatat tagtgcttgg attttaggta
      541 tatcagtagg aaattatgaa gaagatacag aagataagga tgtaatttca aaaattgtaa
      601 ataaaatgat gacaaagttt ggatatttaa gtggagttta ctatataagc caagaaaaaa
      661 tatttaaaca attatattct cattttagac ctgcctacta tagactttta tttaaattac
      721 ctatctataa tccgatttgt gaaaaagtaa aagaagaata taaattagtt tatagaattg
      781 taagtaatgc aatgaaagag ttttgcggat tatttggaga agaaattcag gaagaagagt
      841 tagcatatct tacaatgcac tttgcaacac ttttttcaaa taaaaaagaa tttgatggtt
      901 ttagaaaaag aatagcatta atagtttgtt caaatggcgt tggtagttca gctattttac
      961 atgcagaact tacaaactta tttcctgact tacactttat accaatgtcg gaatcagttg
     1021 gattagaaaa tataagtgaa cctgtagata ttgtatttac tactaattat aacgcagagg
     1081 gtttaaaaac agatgtacca gtaattaaag taagccctgt tatgaccaca aaagaaaaat
     1141 ataagattac acgtgaagtg tatattcaat taggtgatat ttttttaaga caacctaaaa
     1201 tagatgaggt tatggatata ataaaaaaat atgcaaatgt aacctctgaa agtattcttt
     1261 caagtgagct attagcttat tttacacaga tagagaattt tacatctaag gaaggagagg
     1321 ggcctatgct aagtgagatt actaatgaaa cactagtaaa aataaaggta aaagctaaga
     1381 attgggagga agctattaga cagtcagctt ctgtattagt tgaaaataac aaagtaactg
     1441 aagggtatgt agatgctatg attaactcag caaaagcatc tggaccatac atagtaatta
     1501 caaaacatgt tgcacttcca catgctagac cagaagctgg gtcaaaagag attgcaatag
     1561 gcattgctac tttagatagt cctattgagt ttgggaataa cgaaaacgac cctgtaaaat
     1621 atgtattttg tctaagtgca atagacaata atagccattt acgtgctatg tctgagctag
     1681 ttgaactatt ggaagaagat gagtttttta aggttttaga tagtgctaaa gaagctaaag
     1741 aaattattga ttttataaaa acacacgaat tataaaggag gatttaatta tgtataaaat
     1801 tttaatcgca tgtagagcag gagttggctc aagtttaatg ttaaaaataa agactcaaca
     1861 aattattaag gaaaataact ttccaattga agtagaacat ggttcattag attcactaaa
     1921 tggattcaat ggagatgctg tagaaacatt aattgatgtt gctgaagaat taaaaaataa
     1981 aaatttgaag tttgatataa ttgggattca caatattgtt gaccgtaatg aaataaagac
     2041 agcactagaa aaattccttg cttcaaaaga gggataaatt taagatgata aggaggaata
     2101 tttatggagt ttattattag tttgctgagt acacctgctg ttttgcttgg cgtagttgca
     2161 tgtataggat tgattcttca aaagaaatca gctgttgaag tatttacagg ctcttcaaaa
     2221 acacttatcg gttttttgat atttggtatt ggtgcctctg ctatgacagg agcacttcaa
     2281 aatttcaata aactgttcca acatggattt ggaattactg gtgttatagc ttcaccagaa
     2341 gcagctacag cacttgcaca aggtacatat ggttttgcag tttcatgtac attaattctt
     2401 ggattcatat taaatttagt atttgcacgt attacaaagt ttaagaatat tttctttaca
     2461 acaggtcata gtttgttctt ttcttgtgta ttagttttaa ttatgaaagc acatggattt
     2521 gataatacaa tcactatttt aataggagga actatcttag ggtttatgtc tgctgcacta
     2581 cctcaattct gtcaaccatt tatgcgtgaa ttaacaggcg gcgacgaaca agctattggt
     2641 cactttaaca tgattggtta tggtttatca ggttatattg ggcgtttatt ttcaaaacat
     2701 aaagatgata caacagagac tatagaattt cctaagtggt tatcaatatt cagagacttc
     2761 ttaatgggtc tatccatagt tatgttaatc ttattttata ttgcaacatt aaaagctggt
     2821 caagcattta ctcaagaaat tgctggttca acacattggt tggtttttcc gcttatagag
     2881 gcatttacat ttgtggcagg tatgtcaata ttgatgtcag gtgttcgtat gttcttagct
     2941 gaaataactg cagcatttgt aatcatttca gaaaagtata ttccaggttc acgtcctgcg
     3001 ctagatgttc caaccgtatt tccatatgca ccaaatgcag ttattattgg gtttatatca
     3061 gcatatgctg ctggtctatt agcaattgct attatggcat catttcctag tatatttcct
     3121 gtagtaatta ttccagctgc tcacatatgt tttttctcag gaggtacagc tgcaatattt
     3181 ggcaatactt caggaggatg gagaggggct attgctgggt catttgttgt ggggttatta
     3241 cttgcatttt tgccagttat actatatcca gtttatggga ctcttggtat agaaggagca
     3301 actttcccta atattgatta caacgtagtt ggttcgatat tacataatat tttacaattt
     3361 ataaaaccta tttaaactaa attatataaa agtatagtca tagttactat gatttcaagg
     3421 aggatacata ttatggaaaa tacattatct gaatttttag ataatatatc taatgaattg
     3481 tcagaattta ttgcctcaat agatgagaca tctataaata aagcatgcga attaattctt
     3541 gaagctgaaa aaaatcacgg acgtgtacat gtgactggta ttggcaaacc aggacatgta
     3601 tcgggatata tttcatcatt gctttcatct actggaacgt cagcatatat attgcatggt
     3661 acagaagcag tacatgggtc aagtggtcaa gtagttgaag gagatgttgt tattgcaata
     3721 tcaaatagtg gtgaaactca agagttaaaa gcaactctta agactctaaa agtaaatgga
     3781 gcaaagataa ttggtgtaag tggtaatgag agttcatttt taaaaaatat ttctgatata
     3841 ttcctgtttg caggtgttaa acaggaagga gattgtctca ataaagctcc tcgtgcttca
     3901 attttagctg aaacaataat attacaatca ttaagtgttg tattacaata tgctaaaggt
     3961 cttaatacac agcagtattt gaagtggcat ccagcaggaa gtcttggaaa gtctattaga
     4021 gagggaatat aattttatga aatataaagg gattatatgt gcaatgataa caccatttga
     4081 tgaaaatcaa aatattaatt cacaagcaac atgtcaatta attgattatt tgattgaaaa
     4141 gggaatttat ggattattta ttttgggcac taatggtgaa tgtcatgtat taacagatga
     4201 tgaaaaagtt gaatttgcaa agattgttat taatcataca aataatcgtg tacctgtttt
     4261 tgttggaaca gggggtaatt caactcgtga agttgtagat ttatctaaga gaatggaagc
     4321 gattggagca agtgcattat ctattattac accatacttt gttacaccaa cacaacagga
     4381 attaatctta cattacaaaa ctattgctaa ttcagttaat ttacctatta taatgtataa
     4441 tatgccaggt aagactggaa ttaatattga accagagtca gtacgtgaat tatcaaagat
     4501 aaagaatatt gttggaatta aggatagtag tggtaaactt gacaacatga aagcttatat
     4561 tgaagcaacg aagaatgaga attttagtgt gttttcaggc tcagattcac ttattctgga
     4621 tagtctaaaa gctggtagtc aaggagctgt tgcagcaaca acaaacttcc tcacagagat
     4681 tggtatctct atctatgaaa actttatgaa aggggatttg gaagaagctc aaaaagctca
     4741 aagtagtata gaagaacttc gacgtatact gaaacttgga acaattccat ctgttatgaa
     4801 gaaaactatt gtcttgaatg gaattaatgt tggaactgca cgacttcctg taactgaacc
     4861 aactggggaa gtacttgaag aaattaaacg agtagttgaa aattatagga caatacttaa
     4921 taaaagtagt gagaatagat aatataaata gggaaattac actggtatat aattaatata
     4981 tatattataa aacatgacta aaattaatga ataagtaaag tggtttgatg agtatagagg
     5041 tgataatatg aaaaagatga atataaccaa aagaatgtca gaatgtgggg ctcttgcaat
     5101 tgtaagagaa gaaaatctta accgtgcatg tgaaattgct gaaggatgta taaaaggtgg
     5161 tataacagtt atagaaatga gttataccct aaataatgct ggagaaataa ttcaagggct
     5221 taataaaaag tatggtgaaa cactttgtgt gggagcagga acagttttgg atagtgaaac
     5281 agctcgtcat gctatattac atggagctca atttattatt gcaccaaatt acaatgaagg
     5341 tgtagcgaag atttgcaata gatatcagat tccttatgct ccaggatgta catcattgac
     5401 tgaggcagta gatgcactaa gtttaggagc aacttttata aaagctttcc caatatcaga
     5461 tttctatgga cctaaactag taaaggtatt taagacacca atacctgata tgccagtttt
     5521 agcaagtgga gggattaata ttgaaaacct acatatatgg ttagaaaatg gagttgatgt
     5581 atgtggattt ggtggtttat taactaaagg ttctgttgaa gatattgcta aaaatgctaa
     5641 aaagattcgt gaaatcatta aaaatgtaaa aaaaatttaa catagggctg tatcatagta
     5701 tcttataatt actatgatac agccttttta atattattca aatttaaaac agtttacatt
     5761 tctattatat tacatataat ttcagtaatt ttatattaat ttttctatct ggaaaaaaat
     5821 gatttaaaac ttaagttatg atataatttc attaaaagaa ggcgattttt agtaaatatg
     5881 agaaaataat gtgtttttag gggaaaagtt aaatacagat ttaagtgaac aagaatcgat
     5941 aattaagata ttaaatggtg aatagggcat caaaaaaatt actaaattaa aagttttaga
     6001 gggagaatct tagcaagtgt gtgtgaaaat tcaatttcaa acaaaaaata attgtaaatg
     6061 aaatcaattt atacacgttg aagattcact tttatcatta tattaaaaag cagagtgaat
     6121 taaaagtgag tctaaagata ggaggataat taaaacaatg aaagaagaca ttgggttaca
     6181 tcgaatgtgt aaaaaagaac aagcaacaaa gaatgaagtt atatcagagg atattctaaa
     6241 atctatgagt attggggtca gtaaacattt tttagatgat tcatttacaa taatatgggg
     6301 aaatacatca ttctatcaat tattaggata tacagaagaa gaatttttag ttaaattttc
     6361 tagtttaaaa gattattatg aacagaatct ttatcatttt gaatctatga aaaatcattt
     6421 tttagaggct tatcatcatg gcgaaaagag tatagaatat caaatttgca tttcagcaaa
     6481 taatggagac cctgtttggg taataatgac tggtatatta attgaatcac aagataatga
     6541 gaaatcaatg gtctatatat tgtattcaaa aattaatgag ttaaaaataa agcaagaaga
     6601 actatctgat atggaaaaag agagtacaga gaattttaaa tggatgatgt cagagtatgc
     6661 tgggaatgtt tatattagcg atatggatac atatgaatta ctatacttaa ataagcactc
     6721 atgcaatact ttacaggcat cagcaaatga acttattgga agaaaatgtt atgaggtaat
     6781 tcaaggtaga acttcgccat gtccgttttg tacaaattcg tatttaaaaa aagatgacac
     6841 atatgaatgg gaattttata ataaaaaact gaaacgaaca tttataatta aagaccgtat
     6901 gttgaattgg caagggcgga gagttcgtat tgaattatct tatgatatgt atagtgctga
     6961 atataaatta gcaaaaaaag atcaggaaag agaatcaatt ttgaaaacta ttccagcagg
     7021 aatggttaga ttagatgcaa gagattatct tactatactt tggtgtaatg atatattttt
     7081 gaatatgatt gaatatacaa aggaacagtt tgcagaggaa cttaataatc aatgtggata
     7141 tttattttct gaagattata aacgtagtaa gaaactggca caagatttga aggagtctgg
     7201 ggataatatt gtatttgaag caaaaatata tacacgttca agggaagaac gtatatggac
     7261 tgttacattg tgttatatca gtggggaaga cagttgggat ggaataccat ctttttatag
     7321 tataggtatt gacataacaa aggaaagaaa acagattgag aaattacagc atatatcaga
     7381 gaaagatgca ttaacaggaa tttataatcg tgctgagact gaaaggcaga ttaagaaata
     7441 ttttgaaaaa aatttaaatg ttatgggtgc actgtttatg attgatacag ataattttaa
     7501 acagattaat gatacagaag ggcatatgat tggagatata gtacttactg agatggcatc
     7561 aggtatgaaa aaaataatgc gtgacagtga tgttgttgga agaataggtg gagatgagtt
     7621 tacaattttt atgaagaata tttcctcagt taaagatgca gaaaagaaag cagaagaact
     7681 attgtatatg ttccgtcatc tatttcagaa agaaaaaagt tttctaaaag taacttgtag
     7741 cataggaata gctatatatc caaaggatgg tactacattc aaggaaatat atgcaagagc
     7801 tgataaagcc ctgtatcaag caaaaaatat gggaaaaaat aattatgtaa tctacaacca
     7861 agattatttt aaagaattag aagaatttga ttgttcttct cttggtacag ttattgattc
     7921 agaaaaacaa tacgctgaat atcctgataa tcttacacgt tatatattcc gtatgcttta
     7981 tcagactgat gatatagacc aggcgattaa tatgatactt gaggttgttg ggaaacagtt
     8041 tgatgtaagt cgtgtttata tttttgaaaa tacagaagat ggtagatata cttctaatac
     8101 ttatgaatgg tgtaatgaag gaatctcttc tcaaatggct tatttgcaaa acagcaatta
     8161 tcaagactat gaggattatg aaaagctttt tgaggatgaa ttagtttttt attgtcgaga
     8221 tatccataca ttaagtccta aactagaaga attgttttct agtcaaggta tccactctac
     8281 acttcaatgt gcatataaag aagatagagt tttcagtgga tttgttggat ttgatgaatg
     8341 cacaggacta cgtctatggt cacaagagga aataagtaca ttatcattga tttctcaaat
     8401 tatatcaatg tttttacaac gtaaaaaaaa taataaactt aatcaacaga ttcaacaata
     8461 ccagaatatt ttagataata ttgaccagtg tatttgtgtg attaataaag aaaataattt
     8521 tttattatat gtaaataaaa aatttaaaaa tacatattct aaattccaaa ttggacaacc
     8581 ttattatctt gattctatag atattcataa gatatccatt atatggaata aaaaagaagc
     8641 atacctatgt actagcatga tttaatggta aagtaaagtt tatattggac ttaaattaaa
     8701 ttttgtttta aataagatat tattttagtt gattactgaa aagactagaa tctatattaa
     8761 tgaattatac taattttgta ccactattaa aatattgttt gctaaaaata aaaaatagcc
     8821 ttaaaacagt tgattataaa aaattaactg ttttaaggct atttttatct ttgtgacgaa
     8881 atagacactt gccatttaga atgtgacaat atagtagttg tttagaaaag gcacaaagca
     8941 ttatacttta actaaagtaa aatgtatttt gcttttagaa taaaaacaat taggagggag
     9001 ctataattga aaaagataat atatgtatta tttacaattg aactattaat agtatcttta
     9061 ttaggtctaa atatagttaa agataatgaa attaataata ttttatataa cgatacaaca
     9121 agtatatctg taatgtttaa agattataaa aggttaaata aaaattatag taagtggatt
     9181 aaagatattg cagatgaaaa taatgtgact atatcaaagt atgtttttaa taataatgaa
     9241 aaattatcta tatatacgac tgatacaagt ttgaataaca atataaagtt aaaatcagga
     9301 aaatttccta atactaattc aaatgaattt attagtaata taaacactaa taatagtaat
     9361 caagtaggta aattctcaac tatgagtaaa gacatatcaa ttttaattag ggattttggt
     9421 aaaataagtg aagttggaga atcaggaatc ttatatattt caactcaaaa tgaagataca
     9481 ataaataaaa tattaaaaga gttaaatgtg gacagaagta tttttatagc aagattacat
     9541 gatagatatg ttaactcaaa tttatactta aatccttcaa taattagaga tttaatacta
     9601 attatccttt gtttcttggc tacaataata cattattcaa ttagtgtgag tagagaggct
     9661 tctattttaa gattaaatgg atactctaag ctagatataa ttgctagagt tataaaaaat
     9721 atacttagaa ttatgatttt atcatctgtc acagcattat taatatatat aatctatatt
     9781 ttaaaattaa atataggtgt tagagcatgc ttatactttg ctatgttttc gattatatgt
     9841 att
//
LOCUS       DAELNY010000138         9791 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.140, whole genome shotgun sequence.
ACCESSION   DAELNY010000138 DAELNY010000000
VERSION     DAELNY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.140"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            36..1040
                     /locus_tag="KPE55_002792"
     CDS             36..1040
                     /locus_tag="KPE55_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0493840.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIRDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSV
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1166..2278
                     /locus_tag="KPE55_002793"
     CDS             1166..2278
                     /locus_tag="KPE55_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0493841.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2634..3395
                     /locus_tag="KPE55_002794"
     CDS             2634..3395
                     /locus_tag="KPE55_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493842.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3398..4216
                     /locus_tag="KPE55_002795"
     CDS             3398..4216
                     /locus_tag="KPE55_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG0493843.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKTEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4396..4998
                     /locus_tag="KPE55_002796"
     CDS             4396..4998
                     /locus_tag="KPE55_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG0493844.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    5559..5796
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5860..7779
                     /gene="thrS"
                     /locus_tag="KPE55_002797"
     CDS             5860..7779
                     /gene="thrS"
                     /locus_tag="KPE55_002797"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG0493845.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            8547..9638
                     /locus_tag="KPE55_002798"
     CDS             8547..9638
                     /locus_tag="KPE55_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0493846.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         3975 a          946 c         1757 g         3113 t
ORIGIN      
        1 taaaaatatc gtaaatgtat gaggagtgaa atttaatgcg tttcaaaaga atattgatat
       61 tgatgttaac tgtagcaatg atagctggaa tgttggttgg gtgtgctaat aacccaagca
      121 ataatcctag ctctagcaaa gataataaaa gtgatataag cgataaaagt aattcaaaaa
      181 aatctgtatc aatgatacta gatattgaag gaacaaataa tgaagctatg aataattcag
      241 ctttattagc attaaacaat gctcaaaaaa aacttaatat agatacaaac aaagtggaat
      301 cagatgattc ctcaactttt tctaatagta tagatatttt atgtaatgac aattacgatt
      361 taattatagc tgtaggagct agatttgcaa aaccattgga gatggtagcc aagaaatatc
      421 caaagcaaca atttgcaatt atagactatg agtatgacaa gcaaccttct aatataactt
      481 caatttctta tgaagataat aaatctggat acctagctgg attaattgct ggaaaaatga
      541 ctgaaagcga taaggttggt tttataggag gaacaaagag ttctagtagg gataaatttg
      601 aatcaggttt tagagagggt gttaaatttt ctaatagtag tataagggat atttcagttg
      661 agtatgcaga tgtttttaaa gatagcaaat cagtagaatc tatagctaaa aaaatgatgg
      721 ataatggagt ggatattatt ttttctacta ctgaagatga ttcaaaagct gtaatagatg
      781 cagtaagggc aaaaaataaa aaagttatag caacgaataa agatcaacat gagttagctc
      841 cagaaaatgt tataagttct gttgtaaaag attttgagaa tcctacttat aatctgatac
      901 aaagctttgt aaaaggaaat tataaaggcg gaaaagttat agaaaacaca gttaagtctg
      961 gaagtactgg gcttgcccaa aatagttctc aaaatattcc tccagatgtg ttagaatatg
     1021 ttaataagaa caataaataa tctactataa tatccaagaa tacttatgac tttagttatg
     1081 aggtgaaatg gatgttataa tagacatata tagaaagaca tatgcagagg taggttagac
     1141 ctatctaaat cagggagtga attttgtgaa aataaaaagg atattgatgg cttgctttgt
     1201 agtagttttt acaattttta tattagctat atgtaatttt gattttggat ttgataaagt
     1261 aaataagaat aattctaata tagatagtgc taaatcgaaa cagaatgagt ctaaaagttt
     1321 gccaacttca gcagaaaagg ataaatcaaa tagcaaaagt aagaataaag aagatgacac
     1381 agaagatgat tcatatgtaa gaaaaactat tggagtattg ttagataccg aaaacttaaa
     1441 taacgaatca tataataaca atgtactatc agccttaaaa agggctgacg aagattttga
     1501 tgttaggata aaacttgaaa aaccaaagac ttctaaaagt tttaaagaga gtatagaaaa
     1561 actgcataag tatgaaccag atttgataat agcagttgga gccagatttg cagagccaat
     1621 taaagaagct gctactaaat atcccaaaca gcaatttgca atagttgact atacttataa
     1681 agttcagcca gctaatgtaa tttctgtatc atttcaagat aatgttactg gatatttagc
     1741 aggacttatt gcaggtaaaa tgactgatac tgggaaagtc ggttttatag gaggagttga
     1801 gggttctagt agagataagt atgaatctgg atttagaaag gggcttaaaa cttcaaataa
     1861 agatgctgag ttactagtta agtattcaaa tatatttgaa aaccctgaat cagctagaac
     1921 tattgcaaag gaaatgagag attctgatag agatgttgta ttttcagcta cagaggatga
     1981 tagcaaaaga gctattcagg tagcaagaga aaatggtggt aaagttatag ctattaataa
     2041 agaccaatac gatatggctc cagataatgt aataagttca attataaaag atgtagaaca
     2101 acctgtatat gaactgataa agaacgttgc aaaagatggt tttaagggaa gtaaagtaat
     2161 ggaatttaaa ataaaagatg gagaacttgg cttgacttca aagagcagta gaaatatacc
     2221 accagatgtt ttggaataca taaaaaaaga gtataacaaa gtgaaagata catattaatt
     2281 attgatgtag aagattataa aaatatactg cttaagttta gattggtgat aaaaggatat
     2341 atttattaaa tttagtctaa ctgtacaaaa tatattgatt gagtttaggt tagcaataaa
     2401 aagatatatt tattaaattt gtgctaactg tacggaatat actgcttaag tttagattag
     2461 tgataaaaag atatatttat taaatttgaa ccaactgtac aaagctatac taattaagct
     2521 tagattaact atataaaatt ataacaataa gtgtgtgggc agaaaaattc tgctacacac
     2581 tttatttata ttcacataaa aaatttatca aaaaaaagaa aaaggagtaa aaaatggaat
     2641 ttacaagaga accatttata ctagatgacg atttgtgtct agctttggtg gacaaaagaa
     2701 ttagttatct aatggaaaat gaattaaaat taagaaatat agagataata aaaacaatag
     2761 aatgtaaaga attatatgaa agcataaaat atcacccgga tatatgtatt tgtaatttag
     2821 gaaaaggaga tataattgtt gcgccgaatg tatataatca atacaaagag ttattaagta
     2881 gatataaatt taatataatt aagggagaag ctaatttatt aaatagatac ccttatgata
     2941 taccttacaa tattgcgata gttggtgact atgctataca caattttaag tatactgaca
     3001 aagcaatatt agattatatt gaaaataata aacttaaaaa gataaatata gagcagggct
     3061 attctaaatg ctcaatctgt atagtagata gaacgtctat aataacatca gataaaggta
     3121 tttggaaaag tatgaagaat acagaaatag agtgtttatt gatagaaaaa ggtcacataa
     3181 atttatttga gatgaattat ggatttatag gtggatgtac agggttgatt tctaaggata
     3241 aactagcttt ttgtggagat gttaaaaaac atcctgacta tgaacgtata aaaagttttg
     3301 tagaaagtaa aaacaaggag atagttacac ttagctgtga aaatttacta gatataggtt
     3361 ctatagttcc tctcatgaca agaaaggagc gataactttg gttgagaagg ataatatttc
     3421 ttccatgaaa tttttgagga ataataatgt aaacgataga ttaatatata cgaatgacat
     3481 tgtagaagta gtgatagatt ctgcaaagca agaagtgtta aataaaaaaa tacttaatgg
     3541 tactaaaact gaacaaagag ctttaaatat tgatgagata tcaacggaaa taactagtat
     3601 gataataagt ataatgaaag agaaactttt gaaagaatac attctaaaat gctatgggcg
     3661 tacatatcct gaagataaaa ataatatata catatattca ctgaacatat ttgctaaaaa
     3721 agagattttt atgagagaaa cagtatttac tagggtgaga aattatatag aagaaaatga
     3781 ttttataaat gtagaaggtt ttattagatt tagaatgaga gaatttatga aatatatttc
     3841 tgctataggt gatattgcag ttgaggagta tttaataaag aaagaccaag atgaatttat
     3901 aagagtttta aaatacttta tagatactca agaagaaaag attgatttgc ttaaagttca
     3961 tataatggaa gataatacat ttgttttata cgataaaaat ggaaataaga tagacagtat
     4021 agatgatgaa gaaattataa atatggtaat aagagaaaat ttaaattatg aggacttttt
     4081 aataagtacc ttattaactc tatgtcccaa aaaaatagaa atattggatt tgttaaataa
     4141 taattgctca aaggaaatta tagatactgt agaagcaatt tttgaaaata aagttagtat
     4201 aattatggaa aactagaatc taagcatata aaagctatcc atataaaatt aaaaaaattc
     4261 tttttaaatc gacacaaaag acttataatt gtatataaag ctatagtata actagtgtta
     4321 ttaagtataa agaatatcta cttgattaat acaatatagc tggcaaatat acaattgata
     4381 aggagtaatt ttataatgga taatttaatt aggatactga tattagctgt tattggtgga
     4441 tttataggtt atgttacgaa tgtagttgct ataagactga tatttagacc aatagagcct
     4501 attaaaatac ctattttaaa tatagaaata gtaggtctta taccaaagag aagagcagaa
     4561 atagcagcaa atgtaggaga aataattcaa gaagaatttc tttctatgga cgaaatactt
     4621 gcaaatatca ttactgacga agataaggaa gaagttgtaa gatacataaa agcaagggta
     4681 aaaattataa tacatgagaa agtttcattt atcccgagtg gtataaaaaa catgatacaa
     4741 gattatctag gagagataat tgagtctgaa gttaagcaaa gtatagatga attgagtaaa
     4801 aatataataa ataaagcaaa tgaacgtata gatatacaaa aaatggtcga agataagatt
     4861 aatgaactag atttatatga attggaagag ataataataa gaatagctaa gaaagaatta
     4921 aaacatatag agtttttggg gcttgtctta gggtttttaa taggtatagc tcaagggtta
     4981 attactatgt ttatttaaat atatgagaag tagcatttat ttaagtacta attatattta
     5041 cctatttgag tgaattactt attaataaca tttagatatt aattgtattt acctatttga
     5101 gtaaattact tattaatagc atttagatat taattgtatt tacctatttg agtgaattac
     5161 ttattaatag catttagata ttaattgtat ttatttattt gattgtgtta cttattaata
     5221 gtatttaagt atttactgta ctacttatta actatattaa gtattgattg tatttaagta
     5281 tacaaaacct aaaaagagta caaaaattag aagttagttt taataaaaat tagttttagt
     5341 atttatctag tcatatctat catgcatgtg gatacgtttt aaaaatttta aatttatgaa
     5401 aatattataa tatcactata aatcaagaga ttaatttaat ataaaaatat aagaatattg
     5461 gtttacagct gattgagatg aaaaataatg ggtaattaat atattacatt aatgttgatt
     5521 gatgtataac tatctgttat aatgaaaata tattaaaaat catagtgttg aagaggaaga
     5581 gtagagtaga aaccattctc agagaatttg ggtctatggc tgagagccca tgtaataggt
     5641 ctatttgaaa actaccttgg agctgagctt attagcagta tttgttttgt actatacgtg
     5701 aaagtgctcg ggtgacgacc ttatagtcat caagagggtt gtatatactt gtatacgcca
     5761 acttgggtgg aaccgcgaat taatatcgtc ccaaacatat ttggggcgat tttttattac
     5821 aaaaaatcta aaaattattt caataaggag gaaaaaacaa tgattaaagt tgctttaaaa
     5881 gatggctcaa taaaagaatt tgaaaatgct atttctgtta tggatgtagc taaatccatt
     5941 agtgaaggat tagctagaaa tgtggtagca gcgtctgtga acggagaggt tgtaggtctg
     6001 gaccacatta tagatactga ttgtgacttg aatttattta agtttgaaga taaagaaggt
     6061 aaggaagttt ttagacatac atcagcgcat atattagctc aagctataaa aagactttac
     6121 ccagaagcaa agcttgctat aggaccaagt atagaaaatg gattctacta tgatatagat
     6181 ttagaccata gattagttcc agaggaccta gagaaaatag aagctgaaat gaaaaaaata
     6241 gctaaagaag atttaaaaat agaaagattt gaacttccta gaaatgaagc tttagagctt
     6301 atgaaagaac aaggagaaga ctataaggta gaacttatat ctgatttacc tgaaagtgaa
     6361 ataatatctt tctacaagca aggtgatttt actgatttat gtagaggacc acatttacca
     6421 tctactaaaa aggtaaaagc tgttaaatta caaagtgtag caggtgcata ttggcgtggt
     6481 gacgaaaata ataagatgct tcaaagaata tatggaactt cttttgagaa aaacaaagac
     6541 ttagaagagt acttacattt actagaagaa gctaaaaaga gagaccacag aaaattaggt
     6601 aaagaattag gattatttat gatacctgaa gaaggtccag gattccctat gtttttacca
     6661 aaaggtatgg aacttaaaaa tgaattatta aaattctgga gagaaataca cagaaaagct
     6721 ggatatattg agatagaatc tccaatcata ttaaatagaa aattatggga gacttcagga
     6781 cactggtatc attacaaaga aaatatgtat acagttaaaa tagatgatga ggattatgct
     6841 ataaaaccta tgaactgtcc tggtggatta atttactata actctcaact acattcatat
     6901 agagatttcc caatgagagt tgcagagctt ggtagagttc atagacatga gttatctggt
     6961 gcattacaag gattaatgag agttagagca tttactcaag acgattcaca tatattcatg
     7021 ttaccagagc aaattaagga cgaaataaaa ggagtagcta acttaataga tggtatatac
     7081 aaaacttttg gatttgaata taatttagaa ttatctacaa gaccagagaa ctctatggga
     7141 agcgacgaag agtgggaagc agcagaaaat ggattaagag aagctttaga agagttaggt
     7201 ctaccatata ctattaatga gggtgatgga gcattctatg gtcctaagat agacttccat
     7261 ctaaaagatt gtttaggaag aacttggcaa tgtggtacta tccaattaga tatgcaactt
     7321 ccaagacaat ttgataatac ttatattggt caagatggtg aaaagcatag accagttatg
     7381 attcataggg ttgcttttgg tagtatagaa agatttatag gtatattaat agagcattat
     7441 gctggtaaat tcccagtttg gttatctcca actcaagtta agatacttcc tatatctgat
     7501 aaatttatgg attatgcaaa tgaagttaag aaagagttat ttgataaggg tataagagtt
     7561 gaattagatg atagagctga aaaaattgga tttaagataa gagaagctca gcttgaaaaa
     7621 gtgccataca tgttaatagt aggtgaaaaa gaagtagctg ataataatgt ttctgttaga
     7681 tcaagagata aaggtgaaat aggtagtatt aaattagatg aatttatagc tagtatttca
     7741 aaagaaattg aatcaagaga aagtattatt caagactaat atatattatt ttagtagcta
     7801 agatattgtt atataagata agtttaatca atatgttgat atgttgatgc atagtgatta
     7861 aatttatgaa aagatgagta taattagagg tacagttaat catattttta tgattaatgt
     7921 acctctagtt ttttgtatgg tagatttatt aataaatata tcatagaagt gttttgaaca
     7981 tggaaatatt tatgtgcatt ctttaaataa aagtttattt tttgagtgat aattaagtgc
     8041 ttgttggtaa ggttatggtt gtaaaaatca taactttatt ttcatatttg ttccattttc
     8101 ataaaaatta ttatcccttt aagaaaattt cttaatcctc ttgaaatatt tcctttttag
     8161 tctaaagtta aatttctttc ttcaaaatga atatttaaat gaactaaaat ttaagtacat
     8221 tttctaatct tatgagtcta tttttcatag ataattgccc tataatagaa gaatctatgt
     8281 tggagctatg tggtgacaat aacagtattg tcagtgatta attaatgctt tataaatgat
     8341 taattagtgt tttatgttat ttttggactt accctctata catagattaa tttatttaac
     8401 tttagataga gagagaatgt caagtacata cacagacatt agagtttaag aaagtgttta
     8461 tggagcaaga acttttaaaa gaggtagtga cttagtttaa tttcaagaaa tgataaatac
     8521 aaaagaggaa aaagagaggt gttgaaatga tagaaaaagt agaagaaaaa tctaagatta
     8581 ttaaaacatc aaaagaatat aagcgaacta taattgaaga aaaagataaa gaatggacca
     8641 aaaaattaaa tgatatactt agtagaagta gtttttctaa tgatgaatta gataaaatca
     8701 aggcgttaat tccaaaagag attttgacta atgaaaatgt tggagaagtt gtgactgaag
     8761 atggatatgc taagcccgag tatgatgagg tatttaaatc attgttcact ttaaataatg
     8821 aatatgattt acttgtaaat tttataaatg atatcctaaa agatgctcca tatgctaaca
     8881 ggaatatcaa accgttcact caaataaaga gaataattag agttgaaact gacccaacaa
     8941 ttaattacat tggagagaaa agacctagac ttgatatttt ggctgaagat gaagaaaata
     9001 atcatataaa tattgagatg caaagagctt ttgagaacga ttatttggaa agagctgaat
     9061 attatcttag tcgtgtacat ggtagaaagt tagaagaagg taaagagtat aaagaaattg
     9121 gaaaaactat tggaatacat atactaaatc atgtaaaata taatcacata gaagattatg
     9181 taaattgcct gagattaaca atggacgggc atccagatat ttattctagt aaaactgcat
     9241 tatattttat agagcttcca aaaattcata aatctgacta tatagtaaat agaattatgc
     9301 tatggggaaa acttattagt aatccttcta gcttagatgt aagagttata gctaagaatg
     9361 attctattat taaaaaagca cttgatagat taaaagaatt aggtagcaat gaagaatatt
     9421 tattaaatct gaaaagaggt gcttatatca tgaataaaag tagaaatttt aaagaagaga
     9481 ttgaaagaga gacagcaatt agagttgcaa aagaaaaaga ttataaaata attataatgt
     9541 tgaaaaaaaa tggttttaaa ttagatgaca tattaaataa atttaatgat tttgacttaa
     9601 gtgaagatga gataaaagaa gtatatgaaa ataattgatg ataaaaagtg aacttatata
     9661 agtttgctga atcggagtct ggaaatattt atgtgtattt tttaatgaat aaaaatttat
     9721 tttaaaaaca ataatttaga ggtacagtta attatatttt tatgatactg tacttctagt
     9781 tttttctatt g
//
LOCUS       DAELNY010000139         9781 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.141, whole genome shotgun sequence.
ACCESSION   DAELNY010000139 DAELNY010000000
VERSION     DAELNY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.141"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            93..1013
                     /locus_tag="KPE55_002799"
     CDS             93..1013
                     /locus_tag="KPE55_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG0493847.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1128..2285
                     /locus_tag="KPE55_002800"
     CDS             1128..2285
                     /locus_tag="KPE55_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0493848.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2370..3869)
                     /locus_tag="KPE55_002801"
     CDS             complement(2370..3869)
                     /locus_tag="KPE55_002801"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG0493849.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            4465..5889
                     /locus_tag="KPE55_002802"
     CDS             4465..5889
                     /locus_tag="KPE55_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG0493850.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            5924..8053
                     /locus_tag="KPE55_002803"
     CDS             5924..8053
                     /locus_tag="KPE55_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0493851.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            8083..8505
                     /locus_tag="KPE55_002804"
     CDS             8083..8505
                     /locus_tag="KPE55_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0493852.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            8524..9675
                     /locus_tag="KPE55_002805"
     CDS             8524..9675
                     /locus_tag="KPE55_002805"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0493853.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEGILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
BASE COUNT         3976 a         1142 c         1651 g         3012 t
ORIGIN      
        1 atttcaaatt ccttgacagt aataagtaag taattgtata atcaaactgc aatatattaa
       61 taatattagt gaaaatgaaa ggattataaa atatgtataa atcagaaaat ttttcaaaaa
      121 aatctgtatt aataaattca tttttaagta aatttttaaa aataattgga tggggtttgg
      181 taagcttatt tacattatca gccttagtac tttcaactat ttccattata attggatggg
      241 ctgtaatact tcctattcaa gtatgtgcag tatttttact tataggagga gtttgtattt
      301 ttaaaggtaa gagattagga gaccaaattt ctcgttataa aaaatattgt acaattataa
      361 acaatagaaa tattattcca gtagaactta ttgcagaaaa cacatctaaa tcattaaatt
      421 ttataataaa agatgtacaa aagatgatag acaaaaacta ctttataaat acttatattg
      481 ataaaagaaa taatcagatt gtgttaacaa atgaagattt tgttccacca agatatgaag
      541 atgtagcata tgaagtcaaa gaagaagaaa agactgatga atccaatgaa gttgatacaa
      601 ttataaataa aggaatgaat tatctaacac aaataaaaga agctaataaa aatattaaat
      661 cagaatcaat gggcgataaa attgtacaag tagaagatgt tacaagtaaa atatttgatg
      721 ttgtaaaaca tgacccatca aaacttactc agatacaaaa atttatggat tattatcttc
      781 caaccacttt aaaactatta aattcatatc atacattgga agaacaagga attgatagag
      841 aaaatataac aacaactatg gaaagtattg aaaatacaat gaatacgatt gtagtagctt
      901 ttgaaaatca attggactat ctgtttgaag atgaagccat tgatatttct acagatatta
      961 ctgttttaga gaatatgtta gtccaagaag gattaacgag tggtaaattt taacttcaat
     1021 agaaaaaata ttctgttatt tttaaacaat taattagtca taaagtataa aatgtgtatt
     1081 atataagtta atcagtactt tatgaaggaa gaggagagaa agatacaatg aataatttag
     1141 atgatatacc agttatgcca actttgacac ttgacccatt tggagaagaa tcaaacacta
     1201 atgatataga taattctgac ttattaatga aaaaggatga aaaagaccca gaagaagaaa
     1261 aactgtctga atctgaacgc aagatggtaa aggaatttgc ggaaaaaata gatattacaa
     1321 atacaaatat gattttgcaa tatggagctg gagctcaaaa aaaggtagca agtttttctg
     1381 aaacagcttt aaagagtgta aaaacaagag atttaggaga agttggagat atgcttacaa
     1441 atctagttac tgatttaaaa gcattttcag cagatgaaaa agaacaaagt ggttttttag
     1501 gaatttttaa aaaggcaaat aataaaattt caaatttaaa aactaagtat gatagtgcag
     1561 aggtaaatgt ggataaagta agcaaagagc ttcaaaagca tcaggttaaa atattgaaag
     1621 atatagcaat gcttgataag atgtatgaat tgaacttagc ttactcaaaa gagcttacta
     1681 tgtatattat agcaggaaaa caaaaattaa aggatatgaa agaatatgaa atgccaaagt
     1741 tgagagaaaa agctcgttta tctggttcaa cagaagatgc tcaatctgta aatgatatgg
     1801 tttcactttg tgacagattt gaaaagaaga ttcatgactt agaattaact agaatggtat
     1861 cgcttcaaat ggctcctcag ataagattgg ttcaaaatag taataatctg atggcagaaa
     1921 agattcaatc aactatagta aataccattc cattatggaa aaatcaaata gtgcttgctc
     1981 ttggaatttc tcacttaaat caagctatga aagcacagcg tgaagttagt aatatgacaa
     2041 atgagctttt attaaagaat gcagaaactt tgaagatggg aacaatagaa acagcaaaag
     2101 aatcagaaag aggaattgta gatatagaca caattaagaa aactaatcaa tctctaatat
     2161 ctactattga tgaggtagtt aaaatacaac atgatggtag aataaagaga caagaagcag
     2221 aagttgaatt atcaaaaata gaaaatgaac ttaaaagtaa gcttcttgaa tttacagtta
     2281 aataaaaatt atactaatat aaaaagagac tgtctcaaaa tagagataag tctctttttc
     2341 attgtttaaa tgattaaagt atatttatat tataaatttc tattatcttt taacataaca
     2401 tcatgagctc caccttcaac tatactagta gaagatacaa gtgtaagttt agcatttttt
     2461 tgaagttcag gaatgctaag tgctccacag ttacacatag tagaacgaac tttacttaaa
     2521 gaaagagtta cattgtcttt caaagaacca gcataaggca cataagagtc aacaccttct
     2581 tcaaaagaaa gtttcttatc tccaccaagg tcatatcttt gccagtttct agcacgagca
     2641 gaaccttctc cccaatactc tttcatataa ctaccattta tattaacttt gttagttgga
     2701 ctctcatcaa aacgagagaa gtatctacca agcattataa aatctgcacc catagctaaa
     2761 gctaaagtta tatgatggtc ataaacgata ccaccatcag agcaaattgg aatataaata
     2821 cctgtttttt caaagtattc gtctctagct tgagcaactt caattataga agtagcttgt
     2881 ccacgtccta tacctttttg ttcacgagtt atacatatag aaccaccacc aataccaact
     2941 ttaacgaagt cagcaccagc ttcagctagg taaaggaatc cttcgcggtc aactacattt
     3001 ccagctccta ccttaactgt atcaccatat ttttcacgaa tgaaatcaag tgttattttt
     3061 tgccactcag aaaaaccttc tgaagaatct atacataaaa catcagcacc agcttcaact
     3121 aaagcaggaa cacgttcagc aaagtctcta gtattaatac cagctccaac tacatatctc
     3181 ttagaagaat ctaaaagttc aagtggattt tctttatgag aagagtaatc tttacggaat
     3241 accatataca taagattacc attatcatca agtacaggaa gagaattaag tttgtggtcc
     3301 catataatat tgtttgcttc ctttaaagta acatctttgt tagcataaac tatagatgac
     3361 ataggagtca taaattcact aactttagta tctaaatcca tacgacttat acgataatca
     3421 cgacttgcaa caatacccaa taatttacca gttgaagttc catcttcagt aacagctact
     3481 gtagaatgtc cagttttttc ttttaagtca agtatgtctt ttaatgtgtt atcaggttta
     3541 atatttgaat cacttacaac aaaaccagct ttgtgggatt taacacgtgc taccatagca
     3601 gcttcatttt caatagtttg agaaccgtat ataaatgaaa taccaccttc tttagcaagt
     3661 gcaatagcca ttttgtcatc agaaacagat tgcataatag cagaagtcaa aggtatattc
     3721 atataaatat ctgcttcctc gcccttttta aatttagtaa caggtgtttt taaacttaca
     3781 ttagcaggta tacaatcagt agaagagaaa cctggtacaa gaaggtattc actgaaagta
     3841 tgtgatggtg tatcaaaata aaacgccatt ttttaatcac tccttatatt tttatacttt
     3901 ttataattga tagtattaat tataacttat tctttaattt tattaaagta ttttgtcaaa
     3961 tattgagatt tttgaaaaat ttaaactatt ttaattaaat ttgtttaata tatattgcct
     4021 tatagggtaa agttgtatat taaataatgt ttagtgttta atagtagcta atttaaaatt
     4081 aatctttagt gattcatata attacatttg tatagtttac attgataata ttactagaga
     4141 gtgtataaac ctcaaaaata cagataaatt aaatctatat tagtatatcc aaaagtacat
     4201 ttaataaatt tatttaactg aattattatt ttcatttcta ttaaatttaa atattttata
     4261 aaaatagtat tatttacaaa atcatatact ccaaaatact acataaataa aattaaacaa
     4321 aaactaaaat gacaaatgtc attatctaaa gtgacataga aacggtttct taaagttgtt
     4381 actataaaat ataaactcta taataaaagt ataacaaaag ggaatcatat cgatatagaa
     4441 tataattcaa gaggaggtaa aattatgagt agttctaatc aatcgacaat aaagacatct
     4501 atacagcgag tagggaaatt tttaagtggt atggtaatgc caaatatagg agcttttata
     4561 gcatggggtc ttataacagc attatttata ccaacaggat ggatgcctaa tgaaaaattg
     4621 agtactatag gtgacccaat gataaaatac cttttaccac tattgatagc ttatactggt
     4681 ggtaaagcaa tagcaggaca aagaggtggc gtaataggtg cagcagcagc tatgggagta
     4741 attgttggtg cagatatacc aatgtttatc ggagcaatga taatgggacc atttgcagga
     4801 tgggtaatta agaagtttga taaatttgta gatggaaaga taccaacagg atttgaaatg
     4861 ttagtaaata acttttcaat aggtataata ggtatgcttc ttgcaatact aggattttat
     4921 gcaattggac cagcaatagt agcaggaaca gctcttatag aatctggagt tcaatttata
     4981 gttagcaaaa gtttattgcc tctagtttca gtatttatag aacctggaaa agtattattc
     5041 ttaaacaatg caataaatca tggtatatta gggcctatag gtatagctga ggcaaaagaa
     5101 gcaggtaaat ctataatgtt cttactagaa agtaaccctg gaccaggttt aggagttcta
     5161 ttagcatatt ggatgtttag tagaggaagt gtaaaacaat cagcaccagg agctgtaata
     5221 atacatttct ttggtggtat ccatgaaatt tacttcccat atattttaat gaatccagtt
     5281 ttaatattag ctacaatggc aggtggagca gcaggaatat tgactttttc aatattagga
     5341 gctgggcttg tagcagcacc atcaccagga agtatatttg cacttatggc actagctcca
     5401 aagggaggat tacttccagt attagcagga gttgcagtgg caacagttgt atcattctta
     5461 gtggcagcac catttgttaa gagagcttca gcaaatcaaa gtgaagaaga cagtacttct
     5521 ttagaagaag ctaaagcaaa aatgagtgat atgaaatctg caagtaagaa ctctgagaaa
     5581 aatattgagg aaaaacagct tgaagtaaat gagattaaga agattgtatt tgcatgtgat
     5641 gcaggaatgg gttcgagtgc aatgggagct tccagattta aaaatagaat aaaaaactta
     5701 gacttaaata tagaaattac aaattcatct gttgataatc ttccagatga tacacaaata
     5761 gtagtaactc acaatacatt agttgaaaga gttgctaaga ataattcaag tgttgagata
     5821 gtttcaataa acaatttttt aaatgaccca aatttagata cattgtttaa aagattagaa
     5881 tctaaataaa aataaaaagt accactaatt aggagtggat aatttgaaaa aaaagaagat
     5941 tacatctagg caaaaaaaga taatactaat gatagtagaa aattcaaaga aaaacattcc
     6001 aataactatt tctgagattg cagggacttt agagttaagt tcaagaactg tacttagaga
     6061 tatgtctgga attgaaaaat ggtttgatga aaatgatttt aactttgtaa aaaaaccagg
     6121 agtgggattg attttagagg aaaatataga aaatcaaaac tttataattg agttgttaga
     6181 agaagaaaaa atagaaaaag aatacagcaa ggaagaaaga aatttaatta tattatcaaa
     6241 gctactagtt tctaacgagc cagtaaaatc ctattatttt gcaaaaatat taaaggtatc
     6301 tgaaggggta ctgaataatg attttgcctt agcaagtaaa tggttggaaa gatttgatat
     6361 tgagcttgtt agaaaaccag ggcttggtgt ctatttaaaa ggtcaagaaa aaagctttag
     6421 agaggcatat gtaaatctta tatacgattc attcaatgaa aaagaaatac tagatatggt
     6481 cagaaatata agtgaaaata tacaaacaga taaagcaata gaaattttaa gtgaaaatag
     6541 acttttaaat ttaatggaca ggtgcataat aagaaaggtt gaagctacat taacaaagaa
     6601 gctttcagat ttagatgtaa atttagctga tagtgcatat atagggcttg tagttcatat
     6661 ttcattagcc ttacagagga taaaaaatgg tgaaaatata actatggata aagagtttct
     6721 aaaagaatta tctataacag aagagtttaa attagctaaa gaaatcgtaa aaggtatgga
     6781 aacagatttt aacatggata taccagtaga tgaggttgga tatataacta tgcatataag
     6841 aggtgcaaag caaaggtcat cttcaaacca taaggcttta aacttagatg atatagaaat
     6901 aatggaaatt acaaataaga tgatagattt ggcggaagat gaatttaaaa taagtttaaa
     6961 aaatgacgag aggttattta aagacctagc aaatcactta gggccttcta taaatagatt
     7021 gaatatggga ttagaaataa gaaatcctct tttagatgaa ataaaaagca agtattcata
     7081 tgcctatgat ggagttgaaa agatttctag aattataaaa gataaactaa atataaattc
     7141 aatacctgaa tcagaaatag gatatattgc aatgcacttt gcatcagcca tagaaaaaaa
     7201 tcttatgatg aatactaata taaatattgt tgtggcatgt cccactggaa taggtacttc
     7261 aagattctta tcaactaaga ttgaaaataa atttcctaat ttaaatatac ttgaaaccat
     7321 atcagctata aacatagatg aagagtattt aaaggaaaaa gatgttgatt taatagtgtc
     7381 tacagttgag ttaaatacaa gtttaaatta tatatgtgta ggtccattta tgagtttaga
     7441 tgatgaacag ataatcaagg aaaaaataaa atctatagct caaaataaat taatcaattt
     7501 aaatgcgaaa aatgatacta agagtaagaa taaagtatat gaacagataa cagaaagtat
     7561 gaatatagga aaagatattc ttcaattttt agaggaaatt agatttgaaa aatttgaaag
     7621 taaagattta agtgagctta ttgaagactc atcaaaaata tttgcaaaat ctagtgaaga
     7681 tataatctct ataaaagaat cgttaaaaga aagattaaaa atttcaattc catatataga
     7741 agaatcaaaa attctattgt tacattgtat gagtgaaaga atagatatta tgaagttatc
     7801 aataataaaa ctagagaata gaatcgtgct agattcaaat gaagaaatcg acaatgtagt
     7861 ttttatgtta cttcctaaaa attcaccaag ttatcaaaga caaataatga gtgaaataag
     7921 tggttcatta atagacaata taatttttac taataagata aataaattct ctatagaaga
     7981 gatggcatta gaggtaaaag atatagtttt taatttttat acaaacagat taaaagcatt
     8041 tatagataaa taaaattaat attaaaaagg aaggttgata ctatgagtaa agtattgaat
     8101 gagaataata tatttttagg attagatagt gtttcaaagg aagaagctat aactttagca
     8161 ggaagaaaat tagttgaaaa tggatatgta aaagaagaat atataccagc aatgttagaa
     8221 agagaaaaag taatgactac atatatggga atgggagttg ctattccaca tggtgtaaat
     8281 gaagcaaaaa aagaaatatt atcttctgga atagttatat tacaattccc taatggtata
     8341 gattttgatg gagaaaaagc atatttatta ataggaatag ctggtgttgg agatgaacat
     8401 ttagaaatac tatctaatat agcagtagtt ttagatgatg acttaacaga aagactaaaa
     8461 aattcaaatg acaaacaagc ttttatggaa gcttttgcca actaatctta ggagggattt
     8521 aaaatgaaaa aggcaattca gtttggagca ggaaatattg gaagaggttt tattggaggc
     8581 ttgttagtta agtcgggtta tcatgtagta tttgctgatg taaatgaggg gatattaaac
     8641 tcaataaaca aagataaaaa gtatactata cacataagag atgtagaatg tatagatgaa
     8701 gtaattgata atataagtgc ggtttcatct ataaaagaag aaataataga tgagattgta
     8761 caggctgaaa taataactac agcagttgga cctttagttt tgactaaaat agcctctaca
     8821 attgcaaaag gtataaaggc tagaaaagaa aagggattaa caagtaatct aaatataata
     8881 gcatgtgaaa atgcaatata cgcaagttct tctttaaaag aagaggtact taagtattta
     8941 aataaggaag aagtagaata tttagagatg tatgttggtt ttccaaactg ttcagttgat
     9001 agaatagttc ctcctggtaa aaacgaaaat cctcttgatg taacagttga aaatttttat
     9061 gagtggaatg ttgaaaaaca aggatttaaa ggggaaatac caactatagt tggaatgaat
     9121 ttagctgata atcttatggc ttatatagaa cgtaaattgt ttacactgaa tacaggtcat
     9181 gctataacag cttatatagg atatttaaaa ggatataaga ctatagaaga gagtataaaa
     9241 gataaattta tctgtgatat agtcaaaagt gcaatggtag aaagtggaga aggattgatt
     9301 aaaaaataca attttgactc agaagtgcac tataaatata ttgataaaat tttaaataga
     9361 tttaaaaatc catatcttaa tgatgatgta ttaagagtcg gaagggaacc attaagaaaa
     9421 ctaagtgata aagatagatt gattaaacct ttaatgacag caaaatcata tggattatca
     9481 gttgataatt tgattcttgg aataggtgca gcgcttcatt ataataatag tgaagatact
     9541 caaagtgtag aattacaaga gttgataaag tctattgggg taaaaaaagc cgttgctaaa
     9601 atagctaata taagcaatga cgaagaactt ttaaataata tcgaaaaaag ttatatattt
     9661 atgaaaaatt tataaattta cttgaaaaaa agtagttctg ataaagtaac tataatatat
     9721 tattctacac agtttgaatt atttgttgaa taatagagaa ttataatata taattaaaag
     9781 g
//
LOCUS       DAELNY010000140         9717 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.142, whole genome shotgun sequence.
ACCESSION   DAELNY010000140 DAELNY010000000
VERSION     DAELNY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.142"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            150..1514
                     /locus_tag="KPE55_002806"
     CDS             150..1514
                     /locus_tag="KPE55_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0493854.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1536..2027
                     /locus_tag="KPE55_002807"
     CDS             1536..2027
                     /locus_tag="KPE55_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0493855.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EEKAKIFLEMGILTYQKIREDSINDNSFDKLCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIII
                     DLE"
     gene            2300..2971
                     /locus_tag="KPE55_002808"
     CDS             2300..2971
                     /locus_tag="KPE55_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG0493856.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3056..3922
                     /locus_tag="KPE55_002809"
     CDS             3056..3922
                     /locus_tag="KPE55_002809"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG0493857.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3955..5457
                     /locus_tag="KPE55_002810"
     CDS             3955..5457
                     /locus_tag="KPE55_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0493858.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5587..6039
                     /locus_tag="KPE55_002811"
     CDS             5587..6039
                     /locus_tag="KPE55_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG0493859.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6199..7002
                     /locus_tag="KPE55_002812"
     CDS             6199..7002
                     /locus_tag="KPE55_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493860.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7060..7947
                     /locus_tag="KPE55_002813"
     CDS             7060..7947
                     /locus_tag="KPE55_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0493861.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7966..8814
                     /locus_tag="KPE55_002814"
     CDS             7966..8814
                     /locus_tag="KPE55_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0493862.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8877..9566
                     /locus_tag="KPE55_002815"
     CDS             8877..9566
                     /locus_tag="KPE55_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0493863.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         3915 a          915 c         1696 g         3191 t
ORIGIN      
        1 ttaacaaact atttaaatat caagaactgt taaaaaacaa cagcaataaa caaataaaat
       61 agataaatat atccaattaa ggtataatta agttttatat gatatagtta tatttgataa
      121 tttacaatag taaaacttgg agggaaaaaa tgggtgaaaa gaaaataaat aatatgaatt
      181 attcacataa taattattcc aaaaagacag gtgaaccaac tagaaaaata aaaaatagcc
      241 atatcactct taaaagtaaa gaagaaaaaa tttgttctag ttgcaataaa gaaaataata
      301 ctaaggataa ttactgtaaa ttttgtggaa atgaacttta tgaaatagca tcattaagac
      361 cacttgaaac aaaactagac ttgaaatcta aaataaaaga gctttcatat catgcaaata
      421 aaagaggtgt atttttaaca acatttacaa ccatatttat attgtttatt attgcattga
      481 tatttaaagc tattataact attcaattta atgatattag ttatttagta aatccagcac
      541 atataatcct agctctcaac ttgggtcaaa ttagtgtatc tatgtctacc atgatgggtt
      601 ctgggtttat aaatgccaat ataggtttgt tgatattact tataatccct attttggtat
      661 tattaatatc aaacctaatt tttatgagaa aaagatgtag agattcaaaa actgtattag
      721 caaattctct aggagttgga attttttatg gattagtttt agctatttta tctattttta
      781 ctaatgtaaa aactagttca catagtatgt tagaatatgg atatgcatta gaatatagtt
      841 atgaattttt tagtgttctt ttaaatggat tcgtattagg gtttatatgt acatacataa
      901 ctacttataa aaaaagttat gaaaaagaaa atatgtattt atctcttttt tcaaattcta
      961 taagaacatt catattaggt tatgttttag tattagtaat actattggta ctaactatat
     1021 ctgatagtag ttacttaaat gaactagata tgagtagtta ttcaaatgga ttaaatttat
     1081 ttactatact cccacaaata gctagttata tgtgggcttt tgcaaatgga atttcagtaa
     1141 ccataataaa ctcaacagta tcaatgttta ctttaagttc ttcatcttta tttggagata
     1201 ctaaattaat gttttatgca atgggagcat tgtctatgtt aatattatta ttaaatggat
     1261 acaagctaag atttaagtat aatacagata gtataagacc tataatagta tttagtatat
     1321 attatgcatt tttgatggga atattggctt tatttagtac atttatacta gatagtaata
     1381 ttaatttctt taatacaact aattatggaa caactttaat aatgcaattt aaagtcttac
     1441 aagctattgt gatttcattt gtatatagtt ttgtgatatc tttaatagga tataagctaa
     1501 attcagctga ttaaatatga ttggagggaa tttctgtgga aggaaatact aataataaag
     1561 tagatgataa attttctaag gttaagaaaa agtttggtag tggaaaattt aaagttgaat
     1621 tatataaaga aaaatcaaaa ttaaatagag agattgatga tattcaagaa gagaaagcaa
     1681 agatattctt ggaaatggga atactgacat atcaaaaaat tagagaagat tctattaatg
     1741 ataatagttt tgataaatta tgtgatgagt tactagaact tgataagtta atttatgaaa
     1801 aaaatatgga aattaatgaa atggaaatgg cagaaagtaa tgtaacttgt gagtgtggtt
     1861 atgttggaaa tgtaaatgat aaatattgtg ctgaatgtgg taaaaaattt gaattggaaa
     1921 atgaatgtga tgattttata atttgtggtt attgtgaaag tgagatagat acagaggctg
     1981 aattttgtcc atgctgtgga agaaaaatta taatagattt agaataatat tgggaaacct
     2041 atgtatagta tacatgggtt tttttattga aattatttaa atggacttat aacagtagtt
     2101 atataaaatg aaaataaaca ccgaaaaaat gaaataaaat ttcttttaaa tgtaaaaaac
     2161 aagctaatat ataaaataaa tcagtaaaaa tatagatgtg agaaaaaaat atgaaaaaat
     2221 attgcaaaga aaaagttttc atgttattat taatacataa atcaagaaaa taaatttcag
     2281 aaacgaggta atttaaaaaa tgctagataa ggtaaaggga agattaatag tatcatgcca
     2341 agctttagaa gatgaacctc ttcatagtcc ttttataatg gggagaatgg ctaaggctgc
     2401 tatggaaggt ggagctgttg gaattagagc tcaaggagtt gaagatatta ttgaaataaa
     2461 aaaagttact ggacttcctg ttataggtat tataaagaga aactatgaag attcagatat
     2521 atatataacg ccaactaaaa aagaagttga tgaactttta acaacagggt gtgaaatgat
     2581 agcattagat gctaccaata gagttagacc aaataatgaa gacttaaaag aacttataaa
     2641 gtatataaaa gaaaacggtg ttctagtaat ggcagatatt tcaaattatg atgaggctat
     2701 caaagcacaa gagtacggtg tagattgcgt ttcaactaca ctatctggat atactccata
     2761 tactaaaaca ttagaaggtc cagattttgt attaatggaa agattagtta aagatttaga
     2821 aatacctgta atagctgaag gaaaggtaaa tactcctcaa gatttaaaaa aggtctttga
     2881 gttgggtgtt cattcatcag tagtaggttc agcaataaca agaccacaat taataacaga
     2941 aaaatttgtt aaagcaattg aaattaatta aaaattaatt tataaattaa aaaattatta
     3001 tatttaacta tatttgaaaa ttaaaattgg aggtaaagat atgagaacaa cagatatgaa
     3061 gggaatttat tcagcattat tagtttcatt tgataaggaa ggaaatataa atgaaaaagg
     3121 gttaagacaa ataattagac acaatataga tgtttgtaaa gtagatggtc tttatgtagg
     3181 aggaagtaca ggggaaaact tcatgctttc aactgatgaa aagaaaagaa tatttgaaat
     3241 agctaaagat gaagtgaaag aagaaataaa attaatagct caagttggtt cagttaattt
     3301 aaaagaagct gtagaacttg ctaaatttac aactgattta ggatatgatg caataagtgc
     3361 tgttacacct ttttactata aatttgattt tgaagaaata aaacactatt ataatacaat
     3421 aataaattct gtagataata gattaataat atactctata ccatttttaa ctggtgttga
     3481 tatgagttta gaccagtttg gagaattatt tgaaaatgaa aaaataatag gtgttaagtt
     3541 tacagcagca gatttctact tattagagag aatgagaaaa acattcccaa ataaattaat
     3601 atttgcagga tttgatgaaa tgatgttacc agctacagtt ttaggtgtag atggggcaat
     3661 tggttctaca ttcaatgtaa acggtgtcag agctagacaa atattcgaat taactaagaa
     3721 tgaaaaaata tcagaagcac ttgaagtaca acatgtgact aatgatttaa taacagatat
     3781 attaggaaat ggcttatatc aaacaattaa attactactt gaagaacaag gtgttgaggc
     3841 tggatactgt agacaaccta tgaaagaagc aactgatgaa atgaaatcaa gagcaaaaga
     3901 aatatacaga aagtattttt aataaaatac ttttagtatg ggaggattag tcgaatggta
     3961 ggatttactt ggatagattt tgcaatacta gtggtttact tattggcagt tttatttgca
     4021 ggattgttat tttcaaaaaa ggaaatgaag ggaaaagagt tcttcaaagg tgatggaact
     4081 attccgtggt gggttacatc agtatcaata tttgccacat tattaagtcc aatatcattt
     4141 ttatcattag caggaaattc ttatggtggt acttggattt tgtggtttgc acaattaggt
     4201 atgtttatag caataccttt gacaataaaa tttttcttac ctctatatag taggttagaa
     4261 atagatacag catatgaata cttagaaatg agatacgaaa gtaaaggtct tagagtactt
     4321 ggagctttga tgtttataat atatcaaata ggacgtatgt ctattataat gtatcttcca
     4381 tcaatagttc tggctggtct tacaggaata cctgtaaatg tactgataat agttatgggt
     4441 gtaatagcaa taatttactc atatacaggt ggattaaagg ctgtcttgtg gaccgatttt
     4501 atacaaggaa tggtattaat agtaggtgtt acagggacat tgttctattt aatagcaagt
     4561 ataaatggtg gttttggaac agtaatggat actcttacaa ctggacacaa gttcttagca
     4621 agtaatgaag ttatgtttga taaaaatata ctatctacaa gtgcatttat aatatttgtt
     4681 ggagctggtc ttaatacttt ttcatcatat gtatcgagtc aagatatagt tcaaaggttt
     4741 actacaacaa ctgatataaa gcaacttaat aaaatgacat atggaaatgg tgttttatca
     4801 atgggacttg caacagtatt ttatctaatt ggaacttgtt tatttatata ctatactcaa
     4861 aacccagatt tagctcaaac agttcaacaa gaccaagtat ttgcatcata tatagcttat
     4921 gaattaccag tgggtataac aggaatacta cttgcagcga tatatgcagc ttctcaatca
     4981 actttatcga cagggcttaa ttcagttgct acaagttgga ctttagatat acaaactatc
     5041 ataacaaaag atataagtat ggaaaagcaa actaagatag cacaatatat atcattaggt
     5101 gtaggtatac tttctatagc tgttgcaata gtattagcaa atggagaaat aaagtcagca
     5161 tatgaatggt ttaatagttt tatggggcta gtactaggtg ttttagcagg aatatttgta
     5221 cttggtgcat tttgtaaaaa agcaactaaa atgggagcat atgttggatt catagtatca
     5281 gcaattttag ttgtttattt aaaataccgt atgccagaag taacttcttg ggcatattct
     5341 ctaataacaa tcacgacttc tgtagtggta ggtacaatag ttagtgcaat agaagctaaa
     5401 gttaaagcaa aagtatcttt accaaaagct gagactactg tttattatga aaaataatat
     5461 ttaaatgaga aattgtctta tagagtgatt agataaataa tttagttact ctataagatt
     5521 gtataatata ctttgtatac aaaagtataa aaatagactt tataattata tagggagata
     5581 gtgcttatga tatttggaaa tataaatcat agtaagacat attcaacatt gcatgaagat
     5641 ttacttagat gttttgaata tgctaaagat aattcattaa tagattatga aaaaggtact
     5701 tatacaatag atggtgatga tatttttgtg aatattgtag agtataaaac ttgtgaaaaa
     5761 gaagatagat tctgggaagc tcataagaag tatatagatg ttcatttgat gcttgatgga
     5821 tgtgaacgaa tagatataaa ttttatagaa aatttagaac aaaaaatata tgaagaaaag
     5881 ggcgatttct tgcctcttga tggtaaaaat aatggataca tggaacttag aaaaggtgat
     5941 tttttagttt gttatccaga agatgctcat atgacaggaa taaaagtttt agaaaaagaa
     6001 aatatcaaaa aagctatttt taaggttttg gttagataat gataatttat ataaccacta
     6061 gggctgaaaa tagtgattta actttcagcg attagtgaga cttcgtaaat tttagttaga
     6121 ttactatttt cagccttagt tttttatgag aaaattgaaa ataattaaaa gcttaaaatt
     6181 ttgtaaagga gttaacttat ggaaaatata gatgtattct tattaatagg acaaagcaat
     6241 gctagaggat tgggaaatcc aaaagagagc atcattccaa atgaaaattg ttttgaatat
     6301 ttgagtactg atgaaattat aaatatgaga tgtgagctag agacttcaga aggtgatggt
     6361 actatagcac ctgcattttc aaatgaatgg aataagctta ctggaaataa agtttgtttt
     6421 attcacaatg caaaagatgg ttcaagaata aaaaactgga atcatgataa taactggttt
     6481 ctaaatgaca ctatagaaaa atttaataca ggctgtacta ccttaagtaa acattataat
     6541 atagaaaata aatacgttat ttggattcaa ggtgaaagtg atgcaaaata tggtagtgat
     6601 gtattgtact ataaagaaag tctaaaaaaa attgcgtata ggcttaaaga agaatgtatg
     6661 attgataaaa tgtttgtaag tttaactggt tattggcttg gagaagatga atattttatt
     6721 agaacaagac gaattgcagc agcacaggag tcggcttgta gtgaatgtga tatattatgt
     6781 gtaggaagca aaattgctat gcagtttcat gacagaggat taactattga tgatgttcat
     6841 tacactcaag aaggtcttaa tatgttagga gaggacttat gtaaaaatat ttataagtat
     6901 catataacca aagaaaaaag tgtattaagt gatactatag acttatcaga agcaaggaaa
     6961 tatatctgtg aattggaaaa gataagtaaa agatttgtgt aatataaaat tttaaataga
     7021 gttgttaatt tataaaaata atttgtatgg agatatgtta tgaaaaatta cgtttgtata
     7081 gatgttggag gtacttcaat aaaatatggt tttattagag aagatggatt tataattgat
     7141 aaatcaagtc ttgatactga agcaaaggaa aaaggtggag aaggaatatt agccaaaata
     7201 aaagatatag taaaaaaata tatagaggaa aatgaaataa gtggtatatg tatatcaact
     7261 gcaggtatgg ttgacccagt tgaaggtaaa atactatttg cattagaaga attaattcca
     7321 aactataagg gcatgcaatt aaaaaaagaa gtagaaaaag agtttaatat aaaatgtgaa
     7381 gttgaaaatg atgttaattg tgctggactt ggtgaaatgt ggcttggagc aggtagaggt
     7441 gcaacatctt ctatatgttt aacaataggt actggaattg gtggatgtat tataataaat
     7501 aataaactga taaatgggtt tagtaatagt gctggtgaag taggatatat gaatgtaaat
     7561 ggaagttctt ttcaagaatt ggcatctact agcagtctaa ttaaaaaagt agcaaaaata
     7621 aaaaatttga atgaaaatga cttaaatgga aaaataattt ttgatttagc aaagaataat
     7681 gatgaagatt gtttaaaaga actcgataat atgataaaat cattagcagt tggaatcgca
     7741 aatatatgtt atgtaataaa tccagaagtg gtaattttag gtggtgggat tatggcacaa
     7801 gaaaagttcc taaaaccaaa aatagacaaa gcattaaagg aggttcttat agagagagtt
     7861 tacaaaaaca ctaatataga atttgcaaag agacaaaatg atgcaggaat gcttggagca
     7921 ttatataatt ttttaaacaa aatataaaga atagaagggt ttttaatgag tattttagaa
     7981 caattggaat caccaacttt taaagtcaca aaatcagata aattattaat agcttatata
     8041 aaagaaaata tagatgatgt attttataaa ccaatatcac aaatagctaa agagagcaat
     8101 attggtgaag caactataac taggtttgta aaaaaaatga attttaatgg attacaggat
     8161 tttaaagtta ctttagcaca agaaatatca actatcaata aaaaaaatat tataaataaa
     8221 aatatacaaa atgatgagcc agctttggat acagcaaaaa aacttttaag ttcaaatgta
     8281 accactttag agaacacagt agagataatc aatagtaagg atgtacatga ctgcgcaagg
     8341 cttataataa atgcaaagaa agtttacttc ataggtattg gatattctgg tataatagca
     8401 caggattcaa actataaatt tatgagaata ggtcttaatt gtgtaagttt tgacagtagt
     8461 catacaatga taatgatgtc ttccataatg gaagaaggag atttaataat tgcaatttca
     8521 cattctggtg aaacagaaga gattataaaa actgtaaagc ttgcaagagc caataatgct
     8581 aaaattatat caattactga aaataaaaat tctgaattaa aggatatttc agatgtacac
     8641 ttatcttatg tatcgggaga aacagtactg gaaaccggtt caatatcttc taaattagca
     8701 caatttttca ttattgattt agtatataca caggtagtaa aagaactttc taatgaagct
     8761 attgaaagga aaattaaaac tacaaatgct ataaaattgt ttaaaaatga ataaaataag
     8821 aaatttgtaa tgttttatag agctctctta aaaatttagg aatggagaga tattacatgt
     8881 ctaaattaaa agattataat attgataaag taataatttt taataattta agtactaaaa
     8941 cgaagaatca acttaaagaa aaagttaaac taaaaagatt acaaaaaaaa gaaattttat
     9001 tttatgaaaa agatagctta gacagtgttt atatactttt agaaggaaag gtatctattt
     9061 ttaagataag tgaaaatgga gagagaaaag taattttcat attaaatagt ggggaagtca
     9121 taaatgatat tacacttgac gcttctaaaa actctactgt tggctgtgaa gcatttgaaa
     9181 attcaattgt agcttattat agtattgaag aatttttaga aattatgcaa aatgattttc
     9241 aacttacaaa ggatattatt tcttatatgg aaagaagaat aagacgttta tatagacagt
     9301 tgaaaaattc tatatccata aaagtagata aaaaacttgc agcaaaatta tataggttaa
     9361 gtagagaatt tggagtttgc tgtggagaat ggactttact aaatgtaaat attacagtta
     9421 catatttagc tgatatgctt ggctgtaaaa gggagactgt ttccagaatg ataaaggtat
     9481 tgcaaaaaga agaacttatt aaaattgaca ataaaggttt ttatgttaaa gaaactgaat
     9541 tatcaaggta ttttaaaaat aattaattat gattaagtaa gagtattatt agaattttaa
     9601 ttaaataaaa atattattaa gagaatctat ttatagattc ttttttattg tctaatttat
     9661 taatttttat tttaccctat tgaattgtat aaaaaaacaa ataagattct tttttat
//
LOCUS       DAELNY010000141         9662 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.143, whole genome shotgun sequence.
ACCESSION   DAELNY010000141 DAELNY010000000
VERSION     DAELNY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.143"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            285..506
                     /locus_tag="KPE55_002816"
     CDS             285..506
                     /locus_tag="KPE55_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG0493864.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1324..1554
                     /locus_tag="KPE55_002817"
     CDS             1324..1554
                     /locus_tag="KPE55_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG0493865.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            1567..1716
                     /locus_tag="KPE55_002818"
     CDS             1567..1716
                     /locus_tag="KPE55_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG0493866.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            1973..2410
                     /locus_tag="KPE55_002819"
     CDS             1973..2410
                     /locus_tag="KPE55_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG0493867.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            2667..3560
                     /locus_tag="KPE55_002820"
     CDS             2667..3560
                     /locus_tag="KPE55_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0493868.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            3738..4562
                     /locus_tag="KPE55_002821"
     CDS             3738..4562
                     /locus_tag="KPE55_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0493869.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            4661..5269
                     /locus_tag="KPE55_002822"
     CDS             4661..5269
                     /locus_tag="KPE55_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493870.1"
                     /translation="MGKLLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            5373..5549
                     /locus_tag="KPE55_002823"
     CDS             5373..5549
                     /locus_tag="KPE55_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493871.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            6657..7409
                     /locus_tag="KPE55_002824"
     CDS             6657..7409
                     /locus_tag="KPE55_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493872.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            7399..9510
                     /locus_tag="KPE55_002825"
     CDS             7399..9510
                     /locus_tag="KPE55_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493873.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3762 a         1233 c         1244 g         3423 t
ORIGIN      
        1 ctttagtacc taacatttaa ctctacacat atgaaaatcc atacaaaact atctcaacta
       61 agcatgaata tatacaaata ctatttcaaa acactaagaa catatcttca caaataactt
      121 gtaaactttt tcatcagcaa aaaagtaaaa tttttcctat aaaaatttac acaaaaaatc
      181 tttagaaatt actgtttaac tccgatatta tcttagatga tagtattata gctaacaaag
      241 aaagtcgaca aatctttgaa attaaatttt taggaggtaa ttttatggaa gttgtttcaa
      301 caaaaaattt atctagtata aaactaaaat tggatgctgg ttttgatgat aagggtaagg
      361 cagttgttaa aagtaaatca tttgcaaatg taaaagcaga ggcattaaat gaagatgttt
      421 atgctgttgc agaagcaata gcaagtcttc aagagaaccc tattgtagat attttaaaat
      481 tagatagtac atcattatct aaatagtacc ttttaaacgt actctgcttt acaaattaaa
      541 aaaattaact ctttatataa aagattaagt tatataacgc acttaatacg ctatagattt
      601 ggcagattat aacttcaatt ttgattataa ctttaatata ttaatgcttt gaaaaattaa
      661 tatattaaac gtatttcaac ttaaacatgt tagctatttt agatgctacc ctactttaat
      721 ctaattaatt gaaataaatt aaatattttc tatatcagca ataactagag tcaacaatta
      781 aaatactaat attgacaatt gaaacactga tactaacaag caaaacacta atattgacaa
      841 ttgaaatatt aatactaaca agcaaaacac taatattgac aattgaaata ctaatactaa
      901 caagcaaaac attaatattg acaattgaaa tactaatact aacaagcaaa acactaatat
      961 tgacaattga aatactaata ctaacaagca aaacactaat attgacaatt gaaatactaa
     1021 tactaacaag caagacacta atattgacaa ttgaaatatt aatactaaca agcaaaacac
     1081 taatattgac aattgaaaca ctgatactaa caagcaaaac attaatattg acaattgaaa
     1141 tattaatact aacaagcaag acactaatat tgacaattga aatattaata ctaacaagca
     1201 aaacattaat attgacaatt gaaatattaa tactaacaag caaaacatta atattgacaa
     1261 ttaaaatacc aatattaaca agcaaacaat taaaatataa attattatat cggaggtaat
     1321 tatatggaaa ttaagaaaaa attagtcatg agctttaaaa atgtaaagga taaacaagtt
     1381 tctttttcta tagaaaatcc aaaggatgat ttaactgaag aaaatataaa atctgtcatg
     1441 gatttgatag tatctaaaaa tattttttcg gttggaggat ttgacctagc ttctgttgtt
     1501 gaagccaagg ttgtggaaac aaataccact ccttatgact tagttataga atagggggct
     1561 aattttatgg atgatagttt acaactgtta ataaccaatg ttggttttcc tatagctcta
     1621 tccatgtact tattggttag aattgagggt aaattaaata ctttatcaaa cagtattgat
     1681 acattatcca aaaatatttt gatatttaag aagtaatttg acaatatatg taagtcataa
     1741 aataggtcta tttgtaaaat aattagacat attctatgtg aaatactagg atatttggat
     1801 aatttatcca aatatccata gtattttttt gaattttacc acctcaaatt tgaatttcac
     1861 tgttttattt ttgaatttca ctatgcttaa tttgaaattt agaacgcatc tagttggact
     1921 caataagata taatgctatt ctaatgacag taataaaatg aggtgatgat taatgaaaat
     1981 acatgtcgaa caaggtatta attgtgatga tattgaaata accataaaat gttctgctct
     2041 agataataac attgaaaaaa taatttcatt attgagtaca ccgttacttg atataaaagg
     2101 taaaaaatca ggagagattt ataaactcaa tgtctgtgat attttatact ttgagacagt
     2161 tggaaataaa acatttgctt attatgaaga tgatgtattt gaaattgatt taaaaatata
     2221 taaattatca aaattattag ctaatacatc cttctcacga atatctaaat ccatgatttt
     2281 aaatatggac tacataaaaa gtataaaagt aatatcacat aatcgcatgc aagctacttt
     2341 aaaaaacaac gaaaaagtta ttataaatcg tcgttatata aaaaatatta aagaaaagct
     2401 aaacatttaa taaaaatcta aagtaattta ttaatatcac taattgcaaa tttattgacc
     2461 tatttaataa ttaataataa tataatatat tcaatattaa atactaatta attatattta
     2521 gtgtaaaaat aattaatcaa aatataaaaa ataggatatt attataggca ataactaaca
     2581 tcaagcgata acttaataca tataaaaata aataaacaat taataaaaat taaaacttca
     2641 ttataaaacg gaaggatagt gaaaaaatgc aactatataa cacccccttt aatacaaaca
     2701 aacacttaat ctatttcttt acatttacgc tagtattgaa ctggatattc agtcttacac
     2761 ctgcgttgct cggaattaca aatacaatac ttggagaaat cattttttat cttgcatata
     2821 catcaccagc tattgtaggt ctatttttta tttctacaat ctatccacaa agtgcaaaac
     2881 aagattttat ttatagatac ctaagcttta aagaaattag aataaaatgg atactattaa
     2941 caatattatt ttttgcttta acttttatta cttcgttaat aatcggaaat tactttaatg
     3001 taaaaacttc tgatatagat tggatacaca tagcagtgtt tagaccttct aaaatagctt
     3061 atatgctatt agcaagtttt atattgagta tacttagtca agaagctgga tggagaggat
     3121 atgccattga caaattatta gttagatttg gatttatagg ctcttcaatt attttagggc
     3181 ttgtttgtgg aatctggtat ctagggtcgt attttacacc taatcaaata ccatataatt
     3241 tggcacaata ctccctattt gatgcttttt tatttatacc aaacattgta cttttaaact
     3301 ttataattaa ttttgtatat atcaatacaa gtagaagcat tttatcagct gggttagttc
     3361 atatgatgta ctattttttc aacattcaat tacttctaca ctaccctatt aaattatgtg
     3421 taatagctca atatgtacaa ataatatttg gtttaatatt tcttgtatat atgatttctt
     3481 ctaataaatt taaacagaag gtaaattctg aaattgaaca gataaagtcg gatgattttg
     3541 aatttgagtt agactggtaa ttttaatatg aattataaat taagataatc tatgatttga
     3601 tacgattact acttaaatat aatttactct ctataattta ttgcaacatc tggaaaataa
     3661 ttgtatttcc acaaacgctt gaagtatata aaaacaaaat atatactgat tataagtgaa
     3721 aaggaggtca tattttcatg tttaaaatag gagaggtttc aaaactaaca caaatttcta
     3781 ttcgtatgtt gcgttattat gatgaacttg gtattttaaa acctgcagaa atagacaaat
     3841 atacaggtca tagattatac tcagttgaac agatttcaat actacaaaga attgtattat
     3901 tgagagattc aaaatttagc gttgcagaaa tagcaaatat cgtacataat tggaatgatg
     3961 aatttgtaat taaagaattg aatagaaaaa agaatgaaat acaaaaggaa ataaatcagg
     4021 aacaacaaag aataaataaa attgataaat ttatagaagc tataaactgt gataaagatg
     4081 aaattcatta caatgttgtc tttaaaaaga ttccaagtta taaaatcata tcacttagag
     4141 agataattcc agactaccaa tctgaaggta tactctggga aaagctatct aaatttatca
     4201 aagaagaaca tatagaagta tcacgtcaat ccaacaataa catagctttt taccatgatg
     4261 aagaagtcaa agataatggt gtagatattg aagttggaat ggttgtaaag aagattggaa
     4321 agaacaaatc tggatttata tatcgtgaga cagaagaaat tgatatgatg gcatgtacta
     4381 tggtttatgg tccatatgaa aatatagctg gtgcatatga atcgttctgt tattggttag
     4441 ataaaaatag tgattatcaa atatcaggta taaatagaca aatcggacat aaaggtgaac
     4501 ataatgaaat aaatcctgaa aattatctta cagaaattca aatccccctt ataaaagtct
     4561 aatagattag tacttaaata cttaagtact aatcacccat tgacatccca aaattttcat
     4621 gatagtatct aaacgtgtgt taaacatgtt taattttttt atggggaaac ttttaagggg
     4681 aaaaattatg aaatcagatt caacaaagga acaattaatt aatgccacaa tccagttact
     4741 atcttcacat agagatgttt caagcattac ggctagagaa attgtagcta aagcaaatgt
     4801 caatttagct atgattaatt attattttaa atctaaagag gagttaatta atatttctat
     4861 taaaaaattt ttaaagcgtc atatggatga atataaggct acacatgata ctaaaaattt
     4921 agcacctaaa cagcaattaa gggaaatact aattgcattt tttgatttta taggtgaaca
     4981 tagtcaattt ataaagattt ctgtacccta tatattgata caggaagaaa ttgtttatcc
     5041 atttgagata ctacctttaa ttaaggctca ctatgaaaat cagaataaca aaaaaactga
     5101 agctgaatac agaactattg cgtatcaaat aacttcattt attcagttgc tttttcttcg
     5161 ttctaatgct tttttcaagt atagtggtat tgatattctt aatattgaac agagaaatgg
     5221 tttaatagat tttcaattag atttattatt ggtagatatt acaaaataat actatattaa
     5281 ttttttatgt acattttttt attttatggt aacgtttgaa aatattctca ttgactaaaa
     5341 atacttgcat taaataaggg ggaggaattt taatgttttt tacatttata ggagtaatat
     5401 cagcaatttt attgttgtgt acaattatat gtggtttatg gataaaactt aataatcgta
     5461 ataatgactc taattctcat tttacttttt caatactttc aattgctttt tcactgttaa
     5521 ttgtggtggc ctttttagta agaaattaat gtctttgtta tgctgaggtt tattattata
     5581 gatttttatt attgcatgat ttttactata ttatagtatt tttattatat tacagggtct
     5641 tatttattat agtgttttgt tgttacagca ttttattata ttacagagtc ctatttatta
     5701 taggattttt attatattgt aaaatcttat ttattatagg attttgttat attacaatct
     5761 cttatttatt gcagtgtttc tattatattg caaactctta tttattatgg tgttttgtta
     5821 ttgcaacatt tctattatat tgtaaagtct tatttattac agttctttat tacactacag
     5881 acttttatta ttacaatctc ttatttatta cagttcttta ttatactaca gacttttatt
     5941 attacagcat ttctgttaca ttgcaaagtc ttatttatta cagttcttta ttatactaca
     6001 aacctttatt attacaacat tttattattt taacttcttt actataatat agtaatattt
     6061 taatatctca aaataatatc aatcactttt cagcatagtc taaaatactt ccactttcta
     6121 aaattatcta tcatacctat actaataata cttttcaacc tttttattat aaatttcaat
     6181 tgtttttaac aacctattta tttccaaaac tccaatatat tttctcaatt acattgtgtt
     6241 aataacccct aaatcctttc aaattattta caatacattc cattaaataa tcttactgct
     6301 ctataattct ttttttcttt ttaaccctac tatacattat ttacatcatt tttttcaata
     6361 caacattcgt tattctataa cctttttact tttactatat accttttttc taactacacc
     6421 ttcaatgttc cacatcatat tactttacca caacaattaa tacctaaaaa taaccctcta
     6481 aatcctccaa taatataatc aatttaacta taataataac ctctcacact atccaaacaa
     6541 tatattttca aacattacaa aaaaacatcc aactattaca atatagctca tatccctcct
     6601 cccctttcaa atgctatcat tgacttacaa gataaatgaa agtgaggatg atatatatgc
     6661 ttaaagtaaa ccaattatgt aaatcatata aaacaggtag taaaacatat cctgtattaa
     6721 aaaatatttc ttttgaaatc aacaaaggag aatttgtcgc aatcatgggt tcatcaggaa
     6781 gtggaaaaac tactctttta aattgtattt catgttttat cccaattgat aatggggaaa
     6841 taattcttgg aaataataat ataagaaatt taaaagaaaa ggaactttca gaagttagaa
     6901 accaacaact aggatttgta ttccaagatt tcatgctatt tgatggtctt agtgtctttg
     6961 aaaatatatg tgttccacaa attatcaaag gtagtcaagt cttacaaatg gaaaaccgtg
     7021 caattaatct atgtgagcta tttggtattg aacatatcag aaaaaaatac cctgccgaaa
     7081 tatctggtgg agaaaaacag cgtacagcag tagcaagagc actcatgaac aacccagatt
     7141 taatattagc agatgaacca actggtaacc ttgatagtaa gtcttgtcgt acagtcattg
     7201 aatcattttt aaaagcaaag caagaattag atgctactat actcatggtt acacacgata
     7261 gctttgcagc aagcttctgt gaccgagtta ttgtgcttaa agatggaata atttataaag
     7321 aagttgtaaa atcaaattca agacataaat tcctagatga attactagaa gttttaaaag
     7381 aaatgggagg cgctcaagat gacaattaat aaattataca gtaaacttcg agcctataat
     7441 tggaagaact atacaacttt attcttttgt ataatacttt ctgtggtatt agtaacttca
     7501 tatgctctaa tttattttag cccaacaata caaaacatac ttccaactgg tggagatagc
     7561 agaaaacagg ctacaatgat attctcagtt gctatattag gatgcgctgt ctttactaca
     7621 tacgcttctt ccctcttttt aaaatttaaa agcaaggaaa ttggtatttt catggctcta
     7681 ggaacagata agaagaaatt aaaaaaactt ctattttcag agatattgtt aatttcaata
     7741 gtatgctgtt ttttaggtct tatattaagt attcctgtat cttttggtat atggaaaata
     7801 tttcaattag tgattattga taccagagaa atgacatatc attttggaac tcaaggattc
     7861 atatatggat ttatatttag tgtatttgtg acattatgca tatttatact ttctgtaaaa
     7921 tttattaatc gtacaaatat aattgatata ttaaatgacc agagaatatc tgaaccaata
     7981 caggatgtaa aggactggta tggtatatta ggtataattt ttattatact cggtattttt
     8041 ctaggatatc tcgtaccttc actaactata aacaaattaa attatagact accatctgta
     8101 tggagcttta cttatctact ctctcttatt ggaatatata tgattatgac atacatagtt
     8161 gtacatgcta aaagaggaaa acatccagag aaatactata aaaacatcat atcaactagc
     8221 atgatgagat ttatgggaag acaaacagta aaaaacatgt gtgttatttc atttttgatt
     8281 gcaggagcat tatttgctgt attctatgtt cctaatatgg ttacaggtat ctatgatact
     8341 cttaaaaata accctataga ttatactttt acctacaatc aaagagataa acaggtatca
     8401 aaaaaagata ttgaacaatt agcagataaa cacaatataa aaattcaaaa ctatatggaa
     8461 attccttcta tagaattaat tgttgatggt gtagaatcaa tatatcataa agatggaact
     8521 atcgaaaagg tatatatgaa aaaaagctcc tatgatatgt tctttaattc taaagatttt
     8581 tccaagctaa ttggcaaacc aataactata aaaaatggtg aatatcttgc tgttgtaaat
     8641 aaaggaaatg aaaatcaaga ttataaatat accaaattta tatcaagccc agtcacagaa
     8701 aaagaaatcc cctttagata tgctggaaaa ataatattta atggttcttt ttttcaaaat
     8761 agaagtatga acgcctatgt ccttaatgat gaggattata aatcatttac taaagattta
     8821 tccataaaaa attacaataa ttctatacta tttaatgtag ataaagatac ctatgctttt
     8881 gccaatgact taaagaaaga aattataaaa agaacatcta aggaaatggg catgttcagt
     8941 ggttatgatg aatacgtaaa aaaacgttat gaatcaactg gaagaaaata ttttatggat
     9001 gaagaatatc caagtggtga aggtcattta aaactagacc caaacaacaa tcagttgtat
     9061 cttaattgga aatactatcc taactttaaa attttacaaa gccaagatat gataaaaaac
     9121 atggcagtct tcttaatgtt attcatatat gtggcaatca tatgttttac agcagtagga
     9181 atcattgcat atacaagggg tgttactata gctataaatc acaagcaggt ctttatggat
     9241 ttaaatcgat taggagcaaa caatgattat ataaagttct gtattagaaa tcaattaaag
     9301 aaagtatttt cataccctct tattgcagga agttttttaa tttatctgtt taattttatg
     9361 attatgttaa ataatgatgg tagaatcgtc aatagtgagg tcaatgctct tttgataaac
     9421 ttaggtttta tagggatttg tggggtttat atgtacctta tatatctatt aacttataag
     9481 aaattcaaaa aaattattgg tattttataa taaaaaaggt tattagattt ttaaagtaat
     9541 aaaatcattg gtgttttata ataaaaaagg ttattagatt tttaaagtaa taaaatcatt
     9601 ggtgttttat aataagaaag gttattagat ttttaaagta ataaaatcat tactttttat
     9661 aa
//
LOCUS       DAELNY010000142         9567 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.144, whole genome shotgun sequence.
ACCESSION   DAELNY010000142 DAELNY010000000
VERSION     DAELNY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.144"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            508..1686
                     /locus_tag="KPE55_002826"
     CDS             508..1686
                     /locus_tag="KPE55_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0493874.1"
                     /translation="MKYHIFLIITTLLIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            2036..2917
                     /locus_tag="KPE55_002827"
     CDS             2036..2917
                     /locus_tag="KPE55_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0493875.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            3126..3275
                     /locus_tag="KPE55_002828"
     CDS             3126..3275
                     /locus_tag="KPE55_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0493876.1"
                     /translation="MTTVAEGIETSFQKDFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(3535..6102)
                     /locus_tag="KPE55_002829"
     CDS             complement(3535..6102)
                     /locus_tag="KPE55_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0493877.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKA
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQKISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     EPQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(6105..6710)
                     /locus_tag="KPE55_002830"
     CDS             complement(6105..6710)
                     /locus_tag="KPE55_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493878.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(7084..8544)
                     /locus_tag="KPE55_002831"
     CDS             complement(7084..8544)
                     /locus_tag="KPE55_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0493879.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANVLVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8899..9477
                     /locus_tag="KPE55_002832"
     CDS             8899..9477
                     /locus_tag="KPE55_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0493880.1"
                     /translation="MAKSFTEIEKDNIRKRLISECELNWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
BASE COUNT         3274 a         1644 c         1304 g         3345 t
ORIGIN      
        1 ctatacttgg acacataatt attatatttg ctatgtgtaa ttatactata cttggacata
       61 tagttatcat acatactata tgtaattact atacttatac acataactat tatatttgct
      121 atatataatt actatacttg gacacatagt tatcatacat gctatatgta attactatac
      181 ttatacacat aactattata tttgctatat ataattacta tacttgacac atagttatca
      241 tacatgctat atgtaattac tatacttata cacataacta ttatacttac catacataat
      301 tactatattt ggacacatag ttatcataca tactgtatat taagatataa tactttctaa
      361 attttatata acacagacac atgcaataaa aaagaaggta tccataataa gctctttgca
      421 tgtacctaaa taaaaactgt aaaatagaat atataaaatc taatctattt taagtaaata
      481 caatttcact ttaaaagagg atgtactatg aaataccata tatttttaat cataactact
      541 ttattaataa tcgaactatg taacatttac atatgcaaaa tagcatcttt tggaaacaaa
      601 aatataacta tacataagaa ttacaaatat atttttttaa taaaaccatt aaccttcttg
      661 ataataatat tcacaaaatt accattacca ctatattata tattgtttta catagtaagt
      721 cataaattgc tcttaagcag aaaaagttct actaacaacc attttattcc caatatggca
      781 gtaataagtt tttgtatttt acaccttatt actgtaagta caatatcact tgttaaaaat
      841 acaagtccag atataatatt tgcaaatatg acctataagt taattagtat tttaatagct
      901 tctattttag attttatctt atggtactct atgaataaaa taaaagagca tatttatata
      961 ataactcaaa atcaaataga gttcagtcta tttttaaaat ttatctggtt cactttagga
     1021 tatatattat ttgatagtat acaaattaca tttgctccac caagttctct aacttcctac
     1081 tttatattgg gaagtaacat attattaatc ttacaactaa ttatattcat caatcatata
     1141 tattccattg aaaaaaatat ttatttagaa aaagaacatc atagactaaa aatgataaaa
     1201 caagaacaat tactaaaagt aaatttatta aagaaaaaag ctaacattga tgaattaaca
     1261 tcagcatata atcgaaaatt tgcttttgaa aaaatacagc attacattga taacaattta
     1321 ttattttcat tgatatatat cgatttggat aaattaaaga cgataaatga tacatatgga
     1381 catttagctg gggataacta tattattaac ttctcaagta tagttattga aaacttagga
     1441 atagatgaca tatttgctag aataggagga gatgagttta tagttattat gcctaaatct
     1501 aatgaaaaca ccacaaaaga atttatagag aaaataagta aaattatgga taataagata
     1561 tcttttagct atggtattgt agaagtttct agcgaaaaca aaatgactgt agaagaaatt
     1621 attaatattg ctgatattag aatgtacact aataaacaaa ataatcataa aaggaacaat
     1681 aactaaaatg aatatttaca tgagcttact tgtagtctca ctatgttttt tatcttatta
     1741 tctctatcat tataattttt ttaaatatct agacataatg attaataact ctaaaaataa
     1801 atacaaagta tatattgtat actctctttt taattattta atttttatta tttgtactta
     1861 tttaaggtta cacctagtta taaattggac aatttttatg ttaattttat ttatgcaaat
     1921 taaagtaact tacaaacttt ccacaataaa aaaccttttt tattcgttaa actgctccat
     1981 cattggttta gcaataaata tattttttag gtctttaatg tcaatagtgt taaatatgcc
     2041 agcatattta tttgatagta attctattac aaatatttac agagtatttt ctatattgct
     2101 tagttttata gttacaggat tatatttcag ttataaaaat cataaaaata ataagattaa
     2161 ctttgaggca gttttagagg acaaaaaaaa cttaattttc ttattgagcc ttatgtttgg
     2221 aatgtacttg tatctatgtt tgagtttgtc aatatactat actgttggaa atgtattgat
     2281 tttaaaatta tatggaatca agtctgccat aatagttttg gtaggtaact atattgcaga
     2341 aagatatagc tttagaatta ctcaaataaa aggttatgat attaaaaatc aaatagaata
     2401 tgtagaaagt gtaaagacag agtatgaaat taataaattg aatgatattg tatatacaga
     2461 tttactgaca ggcttctaca atagaccttt tgtaaatcaa aaactatcag agtggacatc
     2521 aaatcactac aaatttacct tatgttttac tgatttaaat agcttaaaac ctgtaaatga
     2581 taaatttgga caccaatttg gtgataaata catatccact actgctcaaa taataaaaaa
     2641 acttataaat gtagaaaatt ccttactatt tagatatggt ggagatgaat ttttattcct
     2701 tttacagaat acaagttgtg tagaagcaga aaaaataatg attaatataa attatgaatt
     2761 atcaaaatta agtaatactg acgagtatcc ttatgctctt tccataagct atggattagt
     2821 tgagtgggat ggaatatccg atattgaaac tttaatatca aaggcagatt ctttgatgta
     2881 tgaaaataag cttaaaaata aatctacttt aaactagcta gttaagatat tctacaaaat
     2941 atatgttata agagagtact aagaatgtca aaatatatag attttgcaaa tattgaatac
     3001 agtttactaa tgaatgcact ggatgtaagt gttagtaaac acctattaga tgaacacttt
     3061 actgttgttt gggctaatga ccgttattat gaaatgttcg gctataccta gctaaaaagt
     3121 taaaaatgac cactgtagcc gagggaatag agaccagttt tcaaaaagat ttcttgcaag
     3181 aaatagactg tgatatgtta caaggatacg tgttctctag acctttacca ataaaagact
     3241 ttgaaaaact tatgtttcaa aactcctcca attaacaata taaaattgct tattataagt
     3301 gatattattg tatcaacaaa taaaggaatt gtctcaaaat tgattttttc taatcaaatt
     3361 tttattttga gacattctca tttttttgca taagaaaata cccaccaagc tacggttttt
     3421 cttaccgctg ttcggtgggt attagtagta ttatgaaatc tgaaaacttt aagtattact
     3481 acttccgcta acgcaatcac aaaggtcgaa agcttcaaag cgaaaatagg ctttttactc
     3541 atcaaacgtc accgtacctg ccaacccgtc caccgttatc atttgtccgc tcttaatagt
     3601 tcgtgtggca atgtgtgtag ccataacggc tgggatgcca tattcacgtg cgacaatgct
     3661 tgaatggcta agaggaccac caatgtccgt tacaatcgca cttgcagatg aaaataatgg
     3721 tgtccatgct ggagttgtgg taactgcaac caaaacactt ccaggttgaa agctttcaaa
     3781 atctgctgga ctattcagta cacaagctgg agccgtcaca acaccagtac tggtaccaat
     3841 gcccgtcaac acaattttcc catctttctg cttctgtggt tcttgtgata tagttttttt
     3901 gtttttctct ggcaacttgg atggtgacac ataacccatg tattttttaa gttcagcttt
     3961 tcgaacaggt attatcccgt tcatatcaga tagagacttg tttttatcca actgctctat
     4021 aagtacctcc aattccgatt ttgtgagcca ataaatatca tctaaatgtg aaatagctcc
     4081 tcctcgtatg aatcgttctg agattttctg aaacatacaa cgaatcagag gatgtcccat
     4141 tcccatcaaa taaatagcat tttcacgcat aggagaggtt tcctgtgccc aatggagcaa
     4201 cttaaaaaat agttttttac gcaatccacc tatgtgttgt aaaatctctt cttctgcatg
     4261 ttttctacgc ttttcaaacg tggtctggcg taaaaaagga ctttcccctt ttccttccac
     4321 aaatgttttt acggattcaa aagttggtgt aagtgtttcc tgcggtgtgg aatgcgcaaa
     4381 atcaaactca taagcggtac gaccaaactc tttaaagtac ttattgattc gatttttcca
     4441 ttctatccat acttcctgtg aaactccaac aggcacaact gaagacatga aatcttctgc
     4501 tatctttatt gcagaactgt tttgcagata aaggttgatg ctgttatttt gctttaccca
     4561 ttctgaaata ccccacaacg ccttttccga ttgcagaaca atcgtatcaa acccaagcag
     4621 gaaaacggaa gtatccgtta taccagcacg tcgaactgct ccttgaaata attttgtaaa
     4681 taatgtttca ctgatagaag cagcaggcaa tgtaagctga atccttgtga aataaataca
     4741 tgcggcataa aatattgtct gaataccctc cataatttga tgggcgttca tagtatgcag
     4801 gggcttttct tcccattgtt taatagcatc ctcatatcct tttcgtgccg tttcccagcg
     4861 tgcaacgctg ttcataagtg tcctacgtaa agagcgtggg gaaagggatt tgatagcaat
     4921 caaaagtggc tttgatttag cagaaagata aacatatcca ttaataaccg tatatgcccc
     4981 gttttttggt aataattttt tcgcactttt accaaacata tctatccata aaagctctga
     5041 tgcacggttt acgatttcaa gaccaagcgt ggcaaacaaa ggtgtaacag gatttggcaa
     5101 gtgttctgcc aaacttccct ttgtgtatac aacatcctgt tctggcaata cccattctgg
     5161 tggcagaaca gtaattggac gagcctgaac aatgtacaat ttatcttttt ccagtgccca
     5221 ctctacatcc ataggcattt gataatactt ctcaattttc tttccaagct gtatcagttg
     5281 cattacctga ttacgagtaa gagcatattt ttttctcaac ctttcaggaa ccatggtttc
     5341 ttctgttcct tctgaagtac gaaccgtcat aatctcttta tttgccactt catacgatat
     5401 aattcgttcg gaatccttat ccactacgat tgtatcgggt gtaactaagc tagaaaccac
     5461 cgcttcacca agcccccatg cagcgttaat aatcatttcg ctacgtctac cattgattgg
     5521 gtttaacgta aacataatac cagatgaatc ggaaaatgca agcttttgta ctacaacagc
     5581 aagtgcgaca atctcgtgct ttatattatt cttcacacga taagcaatag cacgagctgt
     5641 ccataaggaa gcccagcatc tctttacagc gtcaataact ttatcttcac cttgtatgtt
     5701 aagataagtt tcctgctgac ctgcaaaaga agcgtcaggt aaatcttcag cagtcgcaga
     5761 cgaacggaca gccaccgata tgttccctaa tccggcataa gccgttttaa ttgcatccga
     5821 cacttcctgt ggcatttctc cattatggaa aagctcccct attttcttag atacatcttc
     5881 aagttggctg gtattattgg aatcaatacc atctagcagt ttattaattc gagactgaat
     5941 atggttcgtc tcaacaaaaa tctggtatga agcagtcgta acatgaaaac cgtctggcac
     6001 agggatacct gctgtcaata gttttgataa agacatccct tttccaccaa caatttcaat
     6061 ggttgcttgt ttatgttcca gtggcaagac ataataaatc ataatcattc acctccatca
     6121 ttcataattc cgtgcagaaa tatattcatt gtttcatcca acattttttc cgaactatcc
     6181 gatggtcgtg acgtgtagct gattgataac atggagttaa ttaacaaacg tgtcacaaga
     6241 gcggtatccg ttttgcgaaa gatgcctgtg gtcacaccat tatcaattac cgattttata
     6301 atatcttgat atgcataacg agcatcttta agtctctttc gatactcttc ttctaaataa
     6361 tcagattctg tatttaacca catcagtaca gttctatact gctctggaaa tctaagatga
     6421 ttaagcatga tttttctaag gcactgttgt ggtgaaggtt caccatcaat aatcctatga
     6481 acttgttgga tagttttttc tatttctttc tcaactgcat atacaacaat ctcgtctttc
     6541 gttttgaaaa aatcgtatag acttgacttt cccatgttgg ctaaaactgc aatttctctc
     6601 attgatgttt tttggaatcc attttcagcc atgagactca aagcaacagc aactattttt
     6661 tctcggcgtt ctgcttgttg ttcgggtgta agcacaattc cttttggcat agggcgatac
     6721 ctccttttag cgaccactgg tcgtttcttt tttatttatc atacagcgac caacggtcac
     6781 ttgtcaaggt taattttttt cattgtatat ggtactcgag ataaaaatgt tcattttaga
     6841 atttgctcta tttaagtaga aataggcttc atcattagaa aacaagacta taaatttgag
     6901 atatttatca tgttcaaatt tcatcaatat caaagtttaa gatattaata aaaaaagaat
     6961 tactaagaaa taacatataa agattcaaaa aaggagttga acttaaaatt taattacaaa
     7021 aaatcaaatt taagacaact cctttattct ttatctctac tattctttat ctctatttat
     7081 ttattacaac tccctaacat ttaccactac tgccttaggt ctagtcatag cctcgattga
     7141 atacttaaca ccttgagttc ccataccaga tgcctttaca cctaaaaatg ggaagtggtc
     7201 tggacctctt tcagtcttat tatttatttg aacagtacca acttcaagtt tatcagctat
     7261 ataaaatgcc ttatcaatat cacttgtaaa tactgatgat tgaagcccat actctgattg
     7321 attagcaatt tcaattgctt gatttatatc tttaactctt ataattggaa gtactggacc
     7381 aaatggttct tcccaagcaa ttttcatatc aacatttaca ttatctaaca atgttggata
     7441 gaataaatta ttttttctaa cattgcctgt tattaacttt gctcctttat gtaatgcatc
     7501 atcaaccaac ccttgaacaa aatcagttgc tttattatct ataagaggag tgataactac
     7561 ttcatccatt ggatttccaa ttgtcaactt ctctaccaat ggctttattt ttccgaccaa
     7621 tttatccgcc acactctctt gtactaaaat tctctttact gccgtacatc tttgtcctga
     7681 atatgaatac gctccagata ctatattctt agctgcaaaa tctaagtctg catcttctaa
     7741 tactatagcc gcatcttttc caccaagctc caataataat ggtaccattc cagatatttt
     7801 agatatatgc tgaccaactt cagtactacc agtaaagtta ataaaattaa ttcctttatg
     7861 cgttacaaca taatctccta tctcactacc cctacctgta atagtattaa gtactccatc
     7921 tggtatccca gcttttctca taatttctac cacatgaagt gcgctaatag ccccttgtgt
     7981 agcaggtttt aatacaactg catttccacc aataagtgct ggagcaatct ttgacattga
     8041 taaatttaca ggataattaa aaggagatat agctaaaaca gtacctaatg gaacccttga
     8101 aacataagac atcttatttc ttgttccacc agggaaatta tctccactaa tggcttcacc
     8161 ctccatattt tttcccacat cagctgtgta tcttaaaaaa tctgctgttc tctcaacttc
     8221 tgcccttgca gattttatgt ctttggctat ttcaagtacc agtacattag ctatctcatc
     8281 tatattttct aacaataaat cagcagcctt atgaaatata ttggctctct tgtatacagg
     8341 catttcagcc catactttta tactctcttt tgtgtttttt ataacctcat ctacctcatg
     8401 ttttgtcata gattgtactt tcccaacaag agaattatct attggtgagt aaatttctat
     8461 tggctttctc gaattagatt ctacccattt tccatttatt aaatttttat atacattttc
     8521 ttttgtttta agttcattaa acatattttt attcctctct ctgatataac tttgtgtatt
     8581 taaattatag ccatttattc gttatgtaaa catttctttt tttcataaac gtttacctat
     8641 aaaaaaaatt ttaagttaac gtttttctac attcttctat tttaataaca tactcgctcc
     8701 aaatctatat aggtcacact ttaatatcaa tatactaata taccaatata ccaatatacc
     8761 aatataccaa tataccaata taaatattat aaacataaat cacttgacaa atttataagt
     8821 agacaatatt atatatctat gactcaaaat atttttaaag tcatatatat catatataga
     8881 tagaaaggat tgataaacat ggctaaaagt tttacagaaa tagaaaaaga caatataaga
     8941 aaaagactaa tttcagaatg tgaactcaac tggtctaaat atggatacaa aaaaactaat
     9001 attgatgaac tttgttcaaa atcaggaatt gcaaagggtt ctttctatac attttttgat
     9061 tcaaaagaaa agttattttt tgaagtatta aatggaatcc aaaacagact tatatcatca
     9121 gtagatgaaa ttttaagtaa catgcctcca aaagaaggct ttgcaaaatc cttaaaaatg
     9181 ttatatcgat tatatgataa aaatccattt ttatattcac caaataatcc tgatttcatc
     9241 agcctaataa ataaactacc tcaagatatg tttttacaac ttgagtccaa taacatacaa
     9301 agtttttacg acttaattga gagacacaat ctcaagttga agatagaaaa agaaaaagtt
     9361 tttggtgttt gtaatgctct actatcaagt ctattggtaa aagaaaatat tgaatataac
     9421 tatattgaag tgtttgactt tatggtggat aatcttgtag aacacatact tgaataacaa
     9481 tttaaaataa tttttctaag tattttttta tactaaatag gattaatatg attgaattaa
     9541 tacaaaaaat aaacaatata atttata
//
LOCUS       DAELNY010000143         9501 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.145, whole genome shotgun sequence.
ACCESSION   DAELNY010000143 DAELNY010000000
VERSION     DAELNY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.145"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(66..338)
                     /locus_tag="KPE55_002833"
     CDS             complement(66..338)
                     /locus_tag="KPE55_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493881.1"
                     /translation="MYDEIIEIYFDYWINEHMYRDILEKDKYYIESWKEVKKINELLE
                     KVLEDDKKYVLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELMD"
     gene            complement(586..1920)
                     /locus_tag="KPE55_002834"
     CDS             complement(586..1920)
                     /locus_tag="KPE55_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0493882.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVQELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFMSY
                     VSIVIEKYKNRNIIDKFKKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEVNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDEEIVKYIQALSIINQENNLY
                     INDSVNTISGIKREIKSIKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(1933..2676)
                     /locus_tag="KPE55_002835"
     CDS             complement(1933..2676)
                     /locus_tag="KPE55_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493883.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(2698..3012)
                     /locus_tag="KPE55_002836"
     CDS             complement(2698..3012)
                     /locus_tag="KPE55_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493884.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(3016..3216)
                     /locus_tag="KPE55_002837"
     CDS             complement(3016..3216)
                     /locus_tag="KPE55_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493885.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            3363..4127
                     /locus_tag="KPE55_002838"
     CDS             3363..4127
                     /locus_tag="KPE55_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493886.1"
                     /translation="MQIGKNLKNIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDSTDNGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            4184..4382
                     /locus_tag="KPE55_002839"
                     /pseudo
     CDS             4184..4382
                     /locus_tag="KPE55_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4379..4870
                     /locus_tag="KPE55_002840"
     CDS             4379..4870
                     /locus_tag="KPE55_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0493887.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            4962..5714
                     /locus_tag="KPE55_002841"
     CDS             4962..5714
                     /locus_tag="KPE55_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0493888.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            5704..6870
                     /locus_tag="KPE55_002842"
     CDS             5704..6870
                     /locus_tag="KPE55_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0493889.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGIPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(6986..7216)
                     /locus_tag="KPE55_002843"
     CDS             complement(6986..7216)
                     /locus_tag="KPE55_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493890.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(7222..7701)
                     /locus_tag="KPE55_002844"
     CDS             complement(7222..7701)
                     /locus_tag="KPE55_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG0493891.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(7701..7916)
                     /locus_tag="KPE55_002845"
     CDS             complement(7701..7916)
                     /locus_tag="KPE55_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG0493892.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(8232..8581)
                     /gene="ssrA"
                     /locus_tag="KPE55_002846"
     tmRNA           complement(8232..8581)
                     /gene="ssrA"
                     /locus_tag="KPE55_002846"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8739..9194)
                     /gene="smpB"
                     /locus_tag="KPE55_002847"
     CDS             complement(8739..9194)
                     /gene="smpB"
                     /locus_tag="KPE55_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG0493893.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         3372 a         1455 c         1071 g         3603 t
ORIGIN      
        1 aaaaggacac ttttaatgtc ctctttaatt tagcattata gaattaactt ataagcataa
       61 gtaaattaat ccattaattc tattataaat tttgctcctg ttttaaatcc taatttataa
      121 ctaagttccc tttcaaactc tattaatgaa ttaaacaaat catttaattc aaccaacaca
      181 tatttcttat catcctccaa aactttttct aataactcat taattttttt aacctctttc
      241 caactttcaa tataatattt gtctttttct aaaatatctc tatacatatg ttcatttatc
      301 caataatcaa aatatatttc tataatttca tcatacattt tagtacctcc ttgactttcc
      361 tcaggcactt aatatatact taagttgcta ggttagtgta catcaagtac ctaatttata
      421 aaggtagttg ttatatttat atggcaacta cctttttcta ataagtgttt aaatctaaac
      481 taaatatatt ttaaatcttt attacatcta attaaaattt atatttatac ttcaacactt
      541 cttgaaacat aatttatcta ttacttaata aatcatgctt cttttttata tctcttgtat
      601 cttcttacat tcttcaaaag aaaacaaatt tcctatatac caatatggtc taaatcctga
      661 ctcgccatct ctatttttag caacaataat ctcacttaaa ttagcaccaa aacctttttc
      721 tcttcgtctc aactctctta tatcctcaat tcccattgtc ttacaataat taagagcttc
      781 tttttctgta ggtctatgaa tatatataac tgaattagaa tccatatata tctgctttga
      841 gcttctcatc actctatcac ctttaggtct ttcatctttc aattcatcat ttaattgagc
      901 aagctgaata actggtatat taaaatctaa agtaatcttt tttatttgcc ttgataagtc
      961 agcaaattta gcttctccta cttctttacc ttcataatcc aaaatttgca aataatcaat
     1021 tatgactaag ctaggcttaa tactctttat ttctctcttt attcctgata ttgtatttac
     1081 agagtcattt atataaagat tattttcctg atttattatg cttaaagctt gtatatattt
     1141 gactatttct tcatcattta gatttctatt tttcatcttt acaactgata ttccagttag
     1201 tgatgctata tttctaaata gaatctgtat atcgctcatt tctccactta taaaaagtac
     1261 tttcttacct tgaaatatta catttctaag tatttgtaac gccaatgctg ttttacctct
     1321 tccactttta gctgctattg tagttagttc acccccatat aaacctccta tagtatcatc
     1381 taagaacttt attccagttt taataccttc atttacttca tcatttatat attcaaaggc
     1441 tctctctgat atggctgtta tacttgaatc cttttttatt aatttctgat tagctttatt
     1501 tatatcattt tctaactgat atataagagt attatcatct atttctccaa gattgaactt
     1561 tttgaattta tcaattatat ttctattttt gtatttttca ataactatac ttacataact
     1621 catgaaattt gatactgttg gaactattgt tgacaaacta gtgatatagc ttatacctcc
     1681 aatattattc aaatgacctt ttctctttaa ttcttctgta agagttatta tatctattac
     1741 ttcatttctc ttatttattt cttttatagc attaaatatg tatttatgtt cctcatgata
     1801 aaacatgtct tcatttaaca tttgctctaa ctcttgtact attgttgaat ctaataaaat
     1861 acttcctaat attgattgct ctgcttctgt atttttaatt aagttgtttg aattatttat
     1921 aatataacct ccttaccact ttaattgtga gtcatctgga ttatatattc ctgtctcttt
     1981 atttatgaca atttccttgc tattataatt tccctctagt atcttagtaa aattacttga
     2041 attcattatc caatcaaaat ttgctttcca tcctgtatta ttatttccat tcagaaaact
     2101 tgttttacta actatattta taacctctaa tacagtttct ttatcatatt cttttattct
     2161 tgaatttatt ctcctctttc ttgtatctgt tagtttcttg acctttgaaa gttcaataca
     2221 agttgagtta aatttgctta tgatttcact atatataata ttattttcat tctttatatt
     2281 cttgattgtt gttgattgat tgttaatagt ctgttgatta ggttgttgat tttctatgtt
     2341 ctcttgataa agctcccagt tttcaatagt tatgactata tacttgtttg ttgtttcgta
     2401 tgttagaaaa ccagcatttt taaaaaccaa caaacaactt ctaaccattt gataactaac
     2461 tcctttacca catctttcag ctattttgct ataggaagaa ataaattgtc cagattttat
     2521 ttttatcctt ttattatcta taaaaacatg gttttcttta tgactagcca taagtaatag
     2581 agttattaat attacttttc tttctggttt aatgtctttc caaattgatt tatccaacaa
     2641 ctccctataa agctttatcc accctttatt gctcaattac aaacacctac tttaaggcta
     2701 taacttgata ccatttgcat tttcaataaa ccaatcatct aatttatttt ttattatgta
     2761 tactcgatta tttatccata tacaagggaa tcctttgttt ttagaatgac ataactgcct
     2821 aaattttgat tctcctatag gataaagctt agatgcttct ttacatgtta taagttcagc
     2881 tttatttctt ttcgcatttt caataatttt atatccttgc tttttttctt gtgcttcttc
     2941 ttcattcttt acaataatgt ttaaagtggc ttgtaatgtc tttaaaacct ctaaattatt
     3001 attctctttc atattttaat cctccttact aaaaaataat tcttctactg ttgtatctaa
     3061 agctctagat agtttaatca tcaaatcatt gcttggatta ttagccttac ctgtttccaa
     3121 gtttgataaa taatatcttg atataccaac tctatctgcc agttcttgtt gctttagacc
     3181 ttttttaatc ctaaatattt ttaaattgat tcccatactt tttcctcctt ttttctagtt
     3241 tacattcgta aactttacat tttaattata atgtcgacat atgtgtactg taaataaaaa
     3301 tatattattt ttctgttctc atttgtgtac ttttgggcta tactttatac gaggtgataa
     3361 tattgcaaat tggaaaaaac ttaaaaaata taagaaaaca aaaagaacta acgcagattc
     3421 aacttgctga aattagtggc atctcaagaa atgctctaat aaattatgaa aatgataaga
     3481 gaatacctag tatagatacc ttatcaaaac tcgctaaagc tttgaaaatt gaaaagacta
     3541 tcttttattt tgatattgaa aatgaagtaa tgactaatga agatgtttta taccttttac
     3601 agtctttatt cccagaagaa tactattctt ttgatgaatt tttaaatgct atttcaaatc
     3661 ttatggaaga tgatgatatt agaattacct caagtttatt gctaggtgcc tggtctgata
     3721 aagaccaata ttataaactt ggtgaaaaat tagaactaaa tgaaaatcaa gtttataatt
     3781 ggatactttc agattgtata tctaatactg aattagattt ttcattacat cctgatgaca
     3841 taaaagaaat tattaaaaat gatgttctta caaaacaatc aattgatgaa ttattagata
     3901 ttggtctatc agaagaaaat atagaaacaa tctatgatta ctttagtgac tcaacagata
     3961 atggaagtaa tattcgacta aaaaaattaa tatctcaaaa agaaaatatt actattaaaa
     4021 ataatgatta ctttaaatta ttacaagaaa ataacgaact aaaggaaaaa aatgaaaagc
     4081 ttgaaaatac acttaaagaa ataaaaaaac tactaactaa aatataaaat aatatcaata
     4141 ctatctaaag caatgataaa aaactatgaa tggagttaca attatgaata aatcatatat
     4201 gctaaaatac acatataaat gccctactaa tttatttcat ggagataaaa aatatactct
     4261 aacatacact aagagagtta tgagtaattt agtatatgat atgggataat ctagaaaata
     4321 actcctttac atgtttagaa gtacaatcat tgtttgatga tatatttaag gtgaaattat
     4381 gatgaataat acaattggag aaaagattaa acaatataga aaatcgataa atatgtcaag
     4441 ggatgagcta gccaacaaga ttggttgttc agttcatgct atagctaaat atgagcaaaa
     4501 tcaaagaatg cctagtttag atatgataag aaaaatagct actgctttat caattgctat
     4561 aaataaactc attgttgaag aaaatgattt agatacttta acaaataata atattaattt
     4621 aaaagtattt gataaagagt tggatttagt aattcacata ttagaaaatt atggttatga
     4681 tataaaaaca gactataaaa aaaagtttga aattacagat aatgcagata caataaaacc
     4741 aattaaccta aacttctctg acttcaaaga cttctccaaa aatctaaatt gggtgataaa
     4801 tggtcttgtt gaaaatttta tacaagaaaa ctctaatggt cttattgaag atgaggatga
     4861 agaatattag tattgcacat tttcaaaata tgagtttacc tattaacttt tatacattca
     4921 tagttaataa ataaaaatta gatatgaaag gagctaaaat catgaataaa tcatatatac
     4981 caaaatacac acataaattc cctactaatc tacctcatgg agataaaaaa tatactttaa
     5041 catacattag aagaatgata agtgaatttg tatatgatat gggtaaccta gaaaataacc
     5101 cctttacatt cccagaagta caaacattac ttgatggtat aaccgtaggc ggacataaat
     5161 taagtgacca aaatcaaata ctaaatataa aatcatcatg ggattatgta ataaaattaa
     5221 gtaacaaaga aaacttatct ttaaataaag atacaatttg ctctatacat aaaatagttg
     5281 caaaagatga agctctaatc gttggagatt tcagaaatgg taatataggt atagctggta
     5341 ctactcaata tgaatgcata gaagccactc tattaaatga tttatttgta aatgacataa
     5401 atatcataaa taaaataact aaccccttag aaaaggctat aataataaat ttatggttat
     5461 cctattgcca attcttctat gatggcaaca aaagaactgc tagacttacc tcaaacctaa
     5521 tacttataag taatgatata ggagttctat caataccagc taaacataaa caagaataca
     5581 acacattaat gctaaacttt tatgaaacac tagaagctga tgaagttata aaattcttat
     5641 tagagaaatg tattacattc ttcgatggat ataactataa atcatataag gagttgttta
     5701 aaaatggcaa ctaaaagagc taatggagaa ggctctatcg gaaaatataa agatggttgg
     5761 cgttctcgta taatgattgg ttataatgaa gatggaaaac caataagaaa agaattttat
     5821 ggtaaaactc aaaaggaagt taaagataaa cttgatgctt ataaaaaaca atattatcta
     5881 agttctgata tttcagatga taaaataact ttagaacatt ggttttacaa ttggttattt
     5941 gaatatagaa taaaagattt aaaaccaaag tcatttgaaa gatatgaaag catctatcgt
     6001 aattatatta aagatactga tttaggcaat gttaagttaa aggacttaag agtatctcac
     6061 atacaaaaat attacaatag attgcttgat tccaataaaa gtatacctac tataaggcaa
     6121 ataaacacta aactgaaaac ctgcttatca gaagctgaaa aacaaggtct tatacaacgt
     6181 aattattgta ctatggtaaa tataccagtt gagaaaaagg aaaataagct tgaaatattg
     6241 actcttgaac aacaaaagag ttttataaaa gctatagatg gtcataaatt agaggtatta
     6301 tttttacttg ctcttggtac tggacttaga ttaggagaat tattaggact taaatggttt
     6361 gatattgatt ttaaaaagtc taatttaaca gttaaaagaa ctcttcaaag aacatatttt
     6421 atagataaaa ctggaaatag agagcttaaa gtattagagc aagagccaaa gacatcaaat
     6481 tcttacagaa ctgtacctat tccaaaagat gttttaaata aattgaaaga gcataaagta
     6541 aatcaaaatt cggatatatt aaaggctgga gaattatata gaaatgataa ttatgtattt
     6601 tgcaatgaat taggtatccc tatagatgat aaaagacctt taaggaatct aaagtctata
     6661 ttaaacagtt tagatataga gcctataaag tttcatggtc ttagaaaaac ttatgctact
     6721 agactttttg agaatgatgt tcctcctaaa actgtacaag ttttaatggg ccattatgat
     6781 atatcaataa ctttaaatat atatactcaa gttatggaag ataagaaggt tgaagctgta
     6841 gaaaagttgg ataaaatatt ttcattgtaa aaatcaaata catataaata ttgtaatttc
     6901 atatagttaa ttacaaaatc aaaataagga tacaaattaa ttttatatta tcttgtatcc
     6961 ttatttcgta tctagtttta attatttatc ttggatagcc tttatgatac attaatccat
     7021 tttctaacat taattcttga gccaagtata tatgaccctt acagtgcata tctgccacac
     7081 cttcacttat aagtttatat gtttcagaag tatataaata atccatactt tcttccaagc
     7141 tcttacctgt ttgcttagaa aattctaata tgattttttt atatagtatt tgtaataatg
     7201 ttttatttgc ttccataatc actatacctc ctcttcatac ctaaacttta aatatttatc
     7261 tattacattt tgattggtta tacatatttg ttcatttggt ttatagtatt ttaatctttt
     7321 taaagcttca tctttttcaa taagattatc ctgatacagt tctattgtat tgtaaatctt
     7381 atcatcagca ataccaccta ttataatgtc atatttcata tatatatcac tcatatttct
     7441 acattctatt ataaaatcaa gccattctaa attataacta ttgaagattt ttaccttgca
     7501 gttttctttt aatttatcaa tatctaaaga ataactattt aaatatgctt ttttacctct
     7561 ttttttaaaa cgctctgtcc agttaattgc ttgtgtcttt atactggtta catagaatcc
     7621 ttttccaaaa tctaaagaat ctcttgaaaa ggtaatacta ggagtcttta ccacacaata
     7681 tgaaccatga tatacaatca ttatatcaca cccttttctt taagtacttg cattaaatct
     7741 tccactatgt aacctttact ttgagaatgt agtgcttcat agcaaggtat tatatagtta
     7801 tctaaaatat cagtatcatg tttaagaagt ttataaactc cttctgaatc tatattcaat
     7861 ttttctgcta aagcttcaat gcaaaaaata gaaaattcta gttttctttc atccattaca
     7921 aacctcaact cctatcaagc ttatataata tatattttta ctaattaaca acttaaacta
     7981 tcttacattt ataaaaatat tatatcataa agtagatatt aaaattctat gttacctatt
     8041 atcataataa acaaataaaa taataaacaa ataaaaactt tttatacaac ctaaatgcac
     8101 cccaatatac ccccaaaaaa acctcttttc ctgtggttgg ggtacggttg gtggatttta
     8161 tctccaaaat aaaaaatact agtcaacaaa gactagtatt tccaagcatt tcatcattct
     8221 ttttttaaaa ttggtggaga cgaggggagt cgaacccctg tccgcaaatc ttttccttgc
     8281 tgcatctaca tgcttagcca acttcactaa tatctcacct cacatagcgc caagtggcag
     8341 gctctatgct tagccagtcc caattaatct acttcaagtc gagacacccc taaagtagtt
     8401 ccctgcataa tgacgtccag tcttagtcgg caggaggact aagagggacg agcagcaact
     8461 aggctgctat cgcgaaatta tcgtctgcgt ttattttaaa gttccccagt ttttacgtga
     8521 cccagagtaa cacgacatgc agcctcaagt tccaaaccca cgtcgaaacc tttacgcccc
     8581 cttgagtagt ttatagtcca gtagactttt cgcaaacttt atcaccattt ttattaaatt
     8641 tttaatgtat atataatata atacttttaa gaataaaaat caatgttatg ttctagataa
     8701 taaagaattt tattctaaaa aatacagttt tatctagttt aatacttacc tgtcatttct
     8761 ctttctatac gtctttgtgc atctttctta gccatatctt gacgtttatc atatagtttt
     8821 ttaccttttg ctaaggcaat ctctacttta actttaccgt tttttagata caaactaagt
     8881 ggtataagtg aatacccctt tatagtagtt gctccaataa gttttcttat ttcatgctta
     8941 tgaagtaata attttctaac tcttaaagaa tcaacattga atatatttcc ttgctcatat
     9001 ggacttatat gaacttgttt taaaaatact tcgctattat ctacagatgc aaatccatca
     9061 cttaggttaa ctttgccttg tcttattgat tttacttctg ttccttttaa ttcaattcca
     9121 cattcataag tttcttctat aaaatattca tgtcttgctc ttttgtttgt tgctaatatt
     9181 tttttaccag ccatataaat ctcaccttct ttgctagaaa tttcttccat aaatgttaga
     9241 aatttactaa atatttaatg ttgccaaatt atttgactaa cttgtatgat aacatattta
     9301 ggcattttag tcaatatttc actgatatat taagttagtt aggctatata taattaagta
     9361 atataaatgt atgaaaatag ctaaaaaact acttcgaata tacttaatac taaactaact
     9421 ctaaccagta atactaattt ataaatttca aaacaaaaat attacatatt ataaatcttc
     9481 tatcttctat cttctatctt c
//
LOCUS       DAELNY010000144         9494 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.146, whole genome shotgun sequence.
ACCESSION   DAELNY010000144 DAELNY010000000
VERSION     DAELNY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.146"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            456..1007
                     /gene="raiA"
                     /locus_tag="KPE55_002848"
     CDS             456..1007
                     /gene="raiA"
                     /locus_tag="KPE55_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0493894.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            1139..3814
                     /gene="secA"
                     /locus_tag="KPE55_002849"
     CDS             1139..3814
                     /gene="secA"
                     /locus_tag="KPE55_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0493895.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            3829..4936
                     /gene="prfB"
                     /locus_tag="KPE55_002850"
     CDS             join(3829..3903,3905..4936)
                     /gene="prfB"
                     /locus_tag="KPE55_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG0493896.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDNDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            5080..7221
                     /locus_tag="KPE55_002851"
     CDS             5080..7221
                     /locus_tag="KPE55_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG0493897.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            7369..8535
                     /locus_tag="KPE55_002852"
     CDS             7369..8535
                     /locus_tag="KPE55_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493898.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      8595..8712
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8837..9442
                     /locus_tag="KPE55_002853"
     CDS             8837..9442
                     /locus_tag="KPE55_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0493899.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT         3954 a         1026 c         1826 g         2688 t
ORIGIN      
        1 gagtatgctt tataactttt aagtagtttt aaatataaat aatgaaagaa aagatagatt
       61 atggaggttt tagagggttt atatttatta aaaaattata aaggtatatt ggcatataat
      121 acacataatt tcctaaaatt accatcttat gacatgaatt cacatattaa taaacaatta
      181 atggagattt ttgtggatag aaataggagt aaaaatagct ttaaataaac aaaaacaaga
      241 gttattaaca gttttatcca cattatccac aggtgtttgt aataatatta atccacataa
      301 gtatcaatgt aactaaaaat gataaataat tctatcaatt gaatagaaaa aatcataaaa
      361 gtaaataata aaaagaggat tatgagagtt cgtgtagaat atattattaa agtataaatt
      421 ttcttatact ggcataattg aagggagatg tatttatgaa cataattata tctggaaaac
      481 aaatcgaatt aactgatgca ataaaaaata aaatcgaaac taaactaagt aagttagata
      541 attacattca tccagacact gatgtaaaag ttacagtaag tgcgagaaaa gcaagacaaa
      601 aaatagaagt aacaatagct actgtaaatg gtcctataat tagagcagaa gatatgcagg
      661 aaaatttata cacagctata gatgttgtat atgataaatt aagtaaacaa ttaaaaaaat
      721 ataaaaagag attacaagat aaacataaag acaacaagag tataagattc caagatgcag
      781 agataaatct agctgatgaa caagaggatt atggggatga aaatcaagaa ttagtaattc
      841 aaagacgtaa gaaatttagt gttaagccaa tgagtgaaga agaagctgta ttacagatgg
      901 aattaatagg acatgatttt tatatgttca agaacataga ttcagatgaa atagcagtag
      961 tttataagcg taataatggt ggatatggta taatagaaca tgaataaagc aatttagatt
     1021 taatcatatt agctatcgat aaataaatta tcgatagctt tttaaattaa tattaaaagt
     1081 atagttttta tggtaaaatg aataatagta caataaagaa cgaggagaga tgtaaaatat
     1141 gagctttatg gataatttat tcaacatggc agataaaaaa gaattaaaaa agttcaataa
     1201 aactgttgat ataatagatt cattagaacc taaatttgaa tctatggcag actcggaact
     1261 taaaaatatg acaaatatat ttaaagaaag attagcaaat ggtgaaagta tagatgatat
     1321 acttccagaa gcttttgcag tagttagaga agtttctaag agagtgttag gacttagaca
     1381 ctacagagtt caaatgattg gaggtatagt actacatcaa ggaagaatag cagaaatgaa
     1441 aacaggtgaa ggtaaaactt tggttgcaac tgctcctgtt tatttgaatg cgcttactgg
     1501 aaaaggtgta catgttgtaa cagtaaatga ttaccttgca aaacgtgata gagaccaaat
     1561 ggcaaaaatc tatgagtttt taggaatgag tgtaggcgtt attatacacg gtcaaaatcc
     1621 aaaagttaga aaagaacaat atgattgtga tataacttat ggtacaaata atgaatatgg
     1681 atttgactat ctaaaggaca atatggtaat acacaaagag caaagagttc aaagaggatt
     1741 aaattatgct atagtggatg aggttgactc tatactaata gatgaggcta gaacaccact
     1801 tatcatatct ggacctggag acaaatcaac tcacttatat tcagatgcaa acacatttgt
     1861 tttaacctta aaaccagatg attatgaatt agaagaaaaa gataaggctg tatctctaac
     1921 agcatcgggt atacaaaaag cagaagtgta ttttaatgtt gataatataa cagatatatc
     1981 tcatacagaa ttataccatc atataaatca agctttaaga gcacatgtaa taatgaagaa
     2041 agatgttgat tatgttgcta aagatggaga aatagtaata gtagatgagt tcacaggtag
     2101 acttatgttt ggtagaagat attcagaagg tttacatcaa gcaatagagg ctaaagaagg
     2161 tcttaaaata caaagagaat ctaaaacact agctactgta acattccaaa attactttag
     2221 aatgtacaaa aaactttctg gtatgactgg tacagcaaaa acagaagaag aagagtttaa
     2281 agcaatctac aaaatggatg ttttccaagt acctactaat aagcttatga taagggaaga
     2341 cttaccagac tgtgtataca aaagtgaaat aggtaaattt aatgctgtag cacaagaaat
     2401 aattgaaagg cacaaagtca atcaaccaat acttgtaggt acagtatcaa tagaaaaatc
     2461 agaattatta tcacaaatac taaagaaaaa gggaataaag cacgaggtat taaatgctaa
     2521 gcatcatgat aaagaggcag aaataatagc acaagctggt agacttggag ctgttacaat
     2581 agctactaat atggctggtc gtggtacaga tatagttcta ggtggaaatc cagacttttt
     2641 aactaagaga gaaatgagaa gaaatggatt taaagaagaa atagtaaata gagtagatac
     2701 tccaatagaa ggtatacctg taaaaggtaa tgaaattcta tttgaagcta gagaagagta
     2761 tgaaaaacta tttgaaaagt ttaagcaaca aacacaagaa gaacaaaaac aagttgtaga
     2821 agcaggtgga cttgctataa taggtacaga aagacatgaa tctcgaagaa tagataacca
     2881 gttgagaggt cgtgctggac gtcaaggtga cccagggagt tcaagattct atataggtct
     2941 tgatgatgac cttatgagat tgtttggtag tgatagaata tctggtattg tagataaaat
     3001 aggtcttgaa gaagacatgc cgatagaaca tagaatatta tctaagtcta ttgaaggagc
     3061 acaaaagaaa gtcgaaggta aaaactttgg aataagaaag catgtacttc aatatgatga
     3121 tgttatgaat aaacaaagag aaataatata tgcagagaga aaacgtgtat tagaaggtga
     3181 agatttacaa gaacaaatac aatcaatgac tcattctata atagaagaag cagttactct
     3241 ttatacacaa gataaaggat ttgatgaaga aggattcaaa gaacatatgt ataatttgtt
     3301 cttgccaaaa ggtagcatag aaataccaga aatagaaaaa cttaatccag ttgaaataac
     3361 agaaaaagtt tatgaaatag ctatgaagat atatacttca aaagaagaac aagttggcta
     3421 tgagagaatg agagaagttg agagagtaat cttacttcaa gctgttgata accattggat
     3481 agaccatata gatgctatgg accaattacg ccaaggtatt ggtcttcgtg ctgtaggtca
     3541 gcaagaccca gttattgctt ataaaatgga aggttttgat atgtttgatg aaatgaataa
     3601 acatatcaaa gaagatactg taagatattt atttaatata acaatagaaa ctccagtgga
     3661 aagaaaagca gttgtagatg ttgaaaatct atcttctcca tcagatggaa cacttccaac
     3721 aagtaaaact gttaaaaaag atgaaaaggt tggaagaaat gacttatgcc cttgtggaag
     3781 tggtaaaaaa tataaaaact gttgtggaag ataatctcgg aggattaaat gttaaagtta
     3841 caagagtata gacatagtat ggagcagatg gcttcaaatt tagaagaatt gagggtttct
     3901 ctttgacata gcttcattga tgaaaaatat acaaattaat gaagaaaaga tgaatcaaca
     3961 agatttttgg aatgataatg aagtcgctca aagagtactt caagaaaata aatctttaaa
     4021 agagacatta gaagaatatg aaagcttaaa aagcttatta gaagatatag aagtattaat
     4081 tgaaataggt ttagaagaag ataatgattc tgtagaaaga gatatagaaa agtccataga
     4141 atcaatggaa gaaaaattat ctgaaatgaa aataaagact cttctaaatg gagagtatga
     4201 taaaaataat gctatattat ctataaatgc tggtactggt ggcttagatg ctcaagattg
     4261 ggctcaaatg cttttgagaa tgtatataag atggtcagaa agtaagggtt ataaagttaa
     4321 gcttttagat ataatatcag accctgaagc agggataaag acagctacaa ttcttgtaga
     4381 aggaacaaat gcttatgggt atttaaagag tgaaaaagga gttcatagat tagtgagaat
     4441 atctccattt gacccatcag gaaaaagaca tacttcattt gcatctatag atgtaacacc
     4501 agagttagat gaaaatatag aagtagaaat taatccatca gatttaaaga tagacacata
     4561 tagagcttct ggagctggag ggcaacatgt aaatacgact gattcagcag ttagaataac
     4621 acatatccct actggtgtag tagttcagtg ccaaaatgaa aggtctcagc atctaaataa
     4681 ggacagagct atgagacttt taatggcgaa attgatagaa ttaaaagagt tagagcaaaa
     4741 ggaaaaaata gaagatatac aaggcaaata ctcacaaata acttggggta gtcaaattag
     4801 gtcatatgtt tttcaaccat ataaattggt taaagaccat agaacaaatg ctgagtttgg
     4861 aaatgtagat agtgtaatga atggaaatat agacttgttt attaatgaat acttaaaaat
     4921 gaataaaata gtttaataaa tatgcactgt aattaagata tatgtataag aaggttagtg
     4981 cttaatatgc acttacagca cttaggctga ggacgataac cctcagcctt cgttctgttt
     5041 tgttatactt atgaaaaact aacattagga gtgaataaat tggatataaa tcaaatctta
     5101 aaaaaagagt ttaatcttag agatgaacaa ataaataata cattaaaact tattgatgaa
     5161 ggaaatacaa taccatttat agctagatat agaaaagaaa tgactggcga aatgagtgat
     5221 gttacactaa gagaatttta tgaaaaatta atgtatttga gaaatcttca aagtagaaaa
     5281 gatgatgtag ttagactaat agatgaacaa gggaaattaa cagatgaaat aacacaaaat
     5341 atagaaaaag caaaaacatt acaagaagta gaagatatat atgctccata taaacaaaag
     5401 aaaagaacta gagcaacaat agcaaaagaa aaaggattag aaaatttagc attgtcaata
     5461 ctagagaata atttagataa tatagaaata gaggcaaaga attatcttga tgaagagaaa
     5521 gaagttcttt ctattgaaga tgctctaaaa ggtgctagag atataattgc agaactagtc
     5581 tcagatgatg ctaagattag aaagtatata agagaattag ctttaagaga aggtatgata
     5641 gttagcaaaa gtgctactga tgagaaatcg gtttatgata tgtattatga ttatagcgaa
     5701 gcagtaaaaa gtatggctcc acatagagtt ttggcaataa acagaggaga aaaagaaagc
     5761 ttcctaaagg ttaagttgga aataaataat gataaagttt taaattacat aataaacgaa
     5821 tatgtaaatg ataaaaattt caaaaataaa gaagaaatcg taagttcaat agaagattct
     5881 tataaaagat taattttccc atcaatagaa agagaaataa gaaatcattt aacagaaata
     5941 gctcaagaaa gagctataag tgtatttgga aagaatgtta aaagtttatt gcttcaacca
     6001 ccagttaaag acaaagtagt tatgggattt gaccctgcat ttagaactgg atgtaaaata
     6061 gctgtagtag ataaaaatgg taaactacta gattatacaa cagtatatcc aacagaccct
     6121 caaaatgatg tagagggagc aaagaaagtt ttaaaaggat tgattgaaaa atacgatata
     6181 gatataattt caattggaaa tggtactgca tctagagaat cagaaacttt tgtatcagaa
     6241 atgataaaag aaatagatag cgaagtgcag tatgtgatag ttagtgaagc aggagcatct
     6301 gtttattcag catctgaact tgcaaatgag gaacatccag atataaatgt atcaataaga
     6361 ggagctatat ctatagctag aagattacaa gacccattag cagagcttgt aaaaatagac
     6421 cctaaaagta ttggtgttgg tcaatatcag catgatttaa acaaaaagag attagaagaa
     6481 gtactagatg gagttgttga agattctgta aatagtgttg gagtagattt aaatactgca
     6541 tcatactccc tactagaaca tgttgcagga ataagtaaag ctatagcaaa aaatataata
     6601 gcatatagag aagaaaatgg agattttacg tcaagagcac aattgaaaaa agtaaaaaga
     6661 ctaggacctc aagcatttac tcaatgtgct ggatttatga gaatcttaga gggtaaaaat
     6721 ccactagata atacaggtgt gcatcctgaa agttatgata tatgtaaaaa aatgattgag
     6781 ataattggat attctttaga tgatgtaaag aataagaata taggtgaaat agatgaaaaa
     6841 ataaaagaaa ttggcttgag ggaattaagt gaaaaactgg aagtaggaca agttacgtta
     6901 aaagatatca tagctgaaat caaaaaacca ggaagagacc caagagaaga aggtataaaa
     6961 cctatactta gaactgatgt cttaaaaatt gaagatatac aagagggtat gactttaaaa
     7021 ggtacaataa gaaatgttgt agactttggt gcatttgtag atattggaat aaaaaatgac
     7081 ggattagttc ataaatcaga aatgagtaat agttttgtga aagaccctat gagtattgtt
     7141 acagtaggtg atatagtaga tgtaaaagtc attggaattg acttaaacaa aaaaagagtt
     7201 gccttatcaa tgaaaaaata gataagttta tgtatggtta tagtacttag attctatatt
     7261 tgtaaataag attactcgag tgtataaatg ttaaaaattt gtaaaagtat atagtaagaa
     7321 aatatattaa aataagaacg atagtcttga aaatcaggag gaaaatgaat gatttttata
     7381 aaaaatggta aaataaatac cataacaaat gggataattc atggagatat acttatagat
     7441 gaaggaaaaa taatagaaat tggagaagat ttgatagctc ctttagatgt agaggtaata
     7501 gatgctagta ataaacttgt gtttcctgga tttatagatg ctcatactca cttaggttta
     7561 tgggaagatg gtatagggtt tgaaggagca gatggaaatg aagagactga cccaataaca
     7621 ccacaattaa atcctataga tggaattaat ccaatggata gaacttttaa agaagctttt
     7681 gaaggtggaa taacttctgt ttgtacaacc cctggaagtg caaatgttat gggtggtcaa
     7741 tgtatagcta taaagacatg tggaaaaaga attgataaaa tggtaataaa aaatccagtt
     7801 gcatctaaaa ttgcttttgg agaaaatcca aagagctgtt atggtcagga tgataaatct
     7861 cctcaaacta gaatggctat agctgcttta cttagagaaa acctaaaaaa agcagaagaa
     7921 tatttagagg atattgatat gtatgagagt catgatgatg aagactgtga aaaacctgaa
     7981 tatgatataa aaatggaaag tttaatacca gttttaagaa gagaaattcc ttttaaggca
     8041 catgctcata gagcagatga tatgtttaca gcaataagaa ttgcaaaaga atttaattta
     8101 aagcttacat tagaccattg tacagaaggt catcttattg tagatgaatt agtggaagaa
     8161 gggtttcctg taatagtagg gccatctttg tcagaaaggt ctaaatttga gcttagaaat
     8221 ttgacattta atacagcagg aatactttca aatgcaggtc ttgatgtatg tataatgaca
     8281 gaccatccag taataccagt tcaatatctt ccaatatgtg caggtattgc agtaaaacat
     8341 ggtatgaaag aagaaaaagc aatagaatca attacgataa atccagctaa ggctttagga
     8401 atagaagata gagtaggctc tatagaagtt ggtaaagatg cagacctagt aatttgggat
     8461 aattctccac tagagattca aagtaatgta ctttacacta taataaatgg caaagtagtg
     8521 tatgagaaaa aataacttga caattattaa aaaattaact aatataagag tgtaaggaaa
     8581 attaaaaaat caaagaagat cttcggggca gggtgaaatt ccctaccggc ggtatagccc
     8641 gcgaactact taggtggttg atttggtgag attccaaagc cgacagtaaa gtctggatga
     8701 gagaagatat ttatggaaga taaataagtt tattttagtt tgtttaaact atatccataa
     8761 aaactcgaag atagggtctt cgagtttttt gtttttccta aaaaatataa acatattgat
     8821 tagggggaaa ataattatgc aaaacacagc aaaaagaggc aatatgattt caacaagaac
     8881 tttagtagta atgtcaattt tatctgcaat atcttattta ttaatgttta ttcaagcacc
     8941 aataccaggt atatttccag atttcttaaa aattgactta tcagatattc cagcaatatt
     9001 cggaggaatg tctctaggac cattagttgg atttggaata gttgtagtaa aaaatgtgtt
     9061 acaagcaata acagcaacaa caactggagg aataggagaa tttgcaaata tattaattgg
     9121 tggttcttat gtaatgatac tttgttattc atataagaga agtggagatt taaaaggtgt
     9181 actgattggt tttgtattag gaacaatatc tatgactata attggttcat tagtaaatta
     9241 ttttgtaatg ttgccacttt atggtcaact tatgggacta gatgctataa taggtttagg
     9301 ttcagctata aatcctcaag ttcatgattt attcactttt gtaatttgga tgatagctcc
     9361 atttaatgtg ctgaaggctg ttataatatc tttagtaaca ttgccacttt ataaaaaaat
     9421 gggaaatata ataaagaaat aattagacaa gaaaatataa aattaaatgt attgaatagc
     9481 cataatttat ttat
//
LOCUS       DAELNY010000145         9414 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.147, whole genome shotgun sequence.
ACCESSION   DAELNY010000145 DAELNY010000000
VERSION     DAELNY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.147"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..1324
                     /locus_tag="KPE55_002854"
     CDS             104..1324
                     /locus_tag="KPE55_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493900.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1361..2710
                     /locus_tag="KPE55_002855"
     CDS             1361..2710
                     /locus_tag="KPE55_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0493901.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            2824..3386
                     /locus_tag="KPE55_002856"
                     /pseudo
     CDS             2824..3386
                     /locus_tag="KPE55_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3494..5827)
                     /locus_tag="KPE55_002857"
     CDS             complement(3494..5827)
                     /locus_tag="KPE55_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0493902.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(5824..6507)
                     /locus_tag="KPE55_002858"
     CDS             complement(5824..6507)
                     /locus_tag="KPE55_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493903.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(6578..7819)
                     /locus_tag="KPE55_002859"
     CDS             complement(6578..7819)
                     /locus_tag="KPE55_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493904.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFKQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(7830..8516)
                     /locus_tag="KPE55_002860"
     CDS             complement(7830..8516)
                     /locus_tag="KPE55_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493905.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
     gene            complement(8754..9098)
                     /locus_tag="KPE55_002861"
     CDS             complement(8754..9098)
                     /locus_tag="KPE55_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493906.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
BASE COUNT         3299 a         1433 c         1169 g         3513 t
ORIGIN      
        1 tattcaatat tcttataaaa aacaaagttc tccatcgaaa attatggcat aataattgct
       61 tattatatta ataaaacaat aaaaagcaat tggaggaata aaaatgcaac aggctttgaa
      121 attaaaatat caaaccaaag aattaggaaa agattttgta atagaaagtt gtaattctat
      181 aaaaccatgg cttataaaca taagaagaga attacataaa attccagaat tagctcttga
      241 agaaaatctt actaaacaga aggtaatttc ttatttaaaa gaaatcggaa ttgattatat
      301 ggaatttaca aaacacaatg gaattatggc atatatttta aaagaaagtg cagataaaac
      361 gatttgtata agagcagata tggatgctct tcctattgaa gaagaaaaca acatacctta
      421 taaatcaata cattctggaa aaatgcatgc ttgtggccat gatgcacata caaccatgct
      481 acttggagca tgtaaagttt tacattcaat aaaagataaa cttaatgtaa atgtaaaatt
      541 cttatttcaa ccagcagaag aaggttttgg aggagcaaaa ttcttagttg aagatggatg
      601 tttagaaaat cctaaggtcg attatatatt cggacttcat gttatgccac atatcgaaac
      661 aggatttatc gaaacaaaat atgatacatt aaatgctagt gtagacacta taaaaataag
      721 tatcaaaggt aaaagagctc atggtgcata tcctgaaaat ggtattgatg caatagttac
      781 agcttcacaa attgttacat cattacaaac tataataagt agaaacttag aaccaaataa
      841 tgctgctgtt ttgactatag gtaaaatata tggtggtgat gcacacaatg taatatgtga
      901 agatgtaaaa ctagaaggca ctttaagaac cttaaatagc aaaacaagaa actttatgat
      961 tgataaaata gctaaaattg ttggagacac tgcatctgca tttggttgtg ttgggacttt
     1021 acatgtaagt gatgaaaact atcctgctgt tataaatgaa aaagaattag tagatacagt
     1081 tatatcaaat actaaagaat tacttggaga ggaaaaattt atattgagac caaacccgtc
     1141 tttaggtggt gaagactttt ctttttacac agaacattgt aaaggagcat tcttccattt
     1201 aggatgtaaa aatgaggaaa agggcttaat ttcacctctt catacctcta gctttaatat
     1261 agatgaagat tgtttaccaa taggtgttat gatgcatgta atgaatactt tgtattttaa
     1321 ttagtacagt aattaataaa agaataggag ataaattaat atggaaaaag ctaagaaatt
     1381 aggtctattt agtatgatat tattagggat taatagcata ataggttcag gtatattctt
     1441 actaccaggt aaagtttata atctagcagg tcaaaacagt atgtttatat acatttttgc
     1501 tacactctta gttctatcaa tacttttatg ttttgcagaa gttggaagca tgtttgataa
     1561 gaatggagga gcatatctat actccaaaaa agcttttgga gattttatag gatttgaagt
     1621 tggtacaatg tcttgggtta ttagaattat atcatggtct actctagcag taggatttgc
     1681 aacagcttta ggttcatttt ggccagaatc tgccactgaa tacaaaggtt atatagctgc
     1741 aatactcgta acactacttt caataaatag cttatttgga attaaaagca caaagataat
     1801 gaataatgta ataactatag ctaaattagt accattaata gtttttataa tagttggaat
     1861 attctttata aaatttgtaa atattgttcc ttcaggaaat gtagttaact ctagtatggg
     1921 tccagccatt atattagtat tttacgcatt taccggcttt gaatcattta ttgtggcaag
     1981 tggagaaatg gaaaatccta aaaaaaatct tccagtcgca ttaataacca caatatttat
     2041 atgtgcaata atttatatat taattcaaat tgtctgtatg ggtatacttg gagatagact
     2101 atttgaaaac agtattccaa ttgcagatgc ttcaagtgta tttttaggca attatggtaa
     2161 agttttcata tctgtagcaa cattaatatc tatatttggt ataaatatag gttcatcaat
     2221 agtaactcct aaatgtggtt cttctctagc agaagaaggt tctcttcctg catttatagg
     2281 aaaaacaaat aaatatggtg ctccatatgt agcaataata atatctttaa tttgttgtat
     2341 accacttgtg ctaactggaa gttttgaaca acttgcagta atgagtgtaa ttgcaagatt
     2401 tgctcagtac ataccaactt gtttatcagt aatagtttta agaaaaagaa cagatgttaa
     2461 ggcctctttt aagattccat ttggtcctgt tataccaatg gtagctattt taggtagctt
     2521 atggttgtta caacaagctt gggcagaaga tatttcaaaa cctataactc aaaatagagt
     2581 tttaatagga ttaggggcaa tgttgttaat agctcctcta tatatattta tgaaaaaaaa
     2641 taaggaaatc gagcataaaa ataaatcaga agattctaaa ttatatattt cgagagaaaa
     2701 aattcaataa tattttacaa actatttaaa aattattttt ctaatactat agaaataatt
     2761 agaaatggag atgttacgta tctaaaagta gttgataaca cggatattgg tacttttata
     2821 gatatgggac ttacaaaaga catactagtt ccttttaaag ctaagactta tccattgttt
     2881 agagatgaaa aatatttatt ctatatatac ttggataaaa gcggaagaat agctgctact
     2941 acagacatag attcatattt agaaacagac catacttaca atgtaggaga tgttgtaaat
     3001 ggagttgtat atggattcca aaccaacaac tctgctatga tatgtgttga caataaatat
     3061 gctggtgtaa tccttcataa tgaatacttt acagaattaa aagctgggga tgttcttgaa
     3121 aatttacatg ttataaaaat atacgatgat ggtaaattag gactttctcc tagaggaaac
     3181 agaaaagatg aacttgatac cttagaaaat aagattttaa gctatttaga aggttcagat
     3241 ggatatatga gattcaatga taaatctgat cctaaagata tttctatctt atttaattca
     3301 agtaagaaaa ttttaaacgt gctcttggag tacttatgaa gaaagactta atttatcaag
     3361 atgaagatgg aacttatcta aagtaaatta tataacacaa aaatcgaagt aaattatgaa
     3421 agatattttc tatttcatat ttacttcgat tttttattta aattactacc tctatttaaa
     3481 ttactactta tatttattgt gcatttacta cctctattat gtctaattca caaatctttt
     3541 taatgggttt tcttactgca agcaaagtta ttattatcat agttactaca atgataaaca
     3601 gtaaatcaaa aggtatttgc cagtctatat ggaattttaa tgttactaag gatacaaata
     3661 tatatctatg taatattaat ccaagtacac tccccaataa acagccactt acagcatatg
     3721 tacttgattc tactattacc atttttctta attgcttatt ggacatgcct attgcacgca
     3781 taataccata gtaatttatt ctacttgtaa cgcttatatt catactgttt atgatattaa
     3841 acatacttat aatcgcaatt ataaatacaa acccatatac aaaaactgcc atagtgtaaa
     3901 atgaattttt agcctctaag ttaccttgtc gcttatctcg aacctctaaa ttatcatcta
     3961 gaacatttct tatattttgg attaaatctt caggagcatc ttcatcaact tgagtatcaa
     4021 ttattatata gtcttttata tctgttatac tagtaaaagt attttcagat gttataatat
     4081 ttccaatata tttgtcttta acatcaaatg aaattgaaga aagtatacct gctacctcta
     4141 tctctttctt agaatttcca tttttaatat atatcttatc tccaatatta aacccatgac
     4201 cttcttctac caatccgata ttttctgact ctaatttatc ttcattgatt tcccctttta
     4261 ctagatactt tttggcccag tcaaattgat ttttttcata agaaattagc tgagatgttg
     4321 cctccttatt attgtatgta gcctctacat ccgtcatcat tctactgtat acttttttta
     4381 ttcctttgat attacttatt tcattaatat cgtctttagt aagaacagac ttaaagtcag
     4441 cgtcaacaat agatatatga gcagcttctg gttttatagg tttaagtgcc tgatacataa
     4501 agtctataaa tacactaaaa cctaaaaaca atacaatact aatagcaaat gaagaagtca
     4561 taagtataaa agattttttt cttgaaaagg catgatttat tcccatagaa acatcaattt
     4621 tagaacttcc tgcgttagca attttacttg cttttccact agttaaaatt tctttgaaca
     4681 ttagattacc acgtatagca cttagtggag acaccccaga agcttttttt gctggaacaa
     4741 tagctgataa tattactgtt aaaaatccta ctattattcc aaacacaata ccaataaagc
     4801 taacctttaa aattggcatc gaaccaaata actctgtatt cacatatttg agaaatatag
     4861 aggatataat ggttacaata catcctaata acaatcctag tggaatagcc tttacactta
     4921 gaattaaact ttctaaaaca acaaattttt tgacttggag ttttgatgct cccaagcaac
     4981 gtaatagtcc aaaaaacttt attctctcta ttacacccat attaaagcta ttatatatca
     5041 tgataatact tgctattaac acaagtataa aaagtcctcc tgctgttaag taaagtggtt
     5101 tagaataatc agagtcacct tgacctataa tagaaagtaa tttttcattt ttaccgattt
     5161 gtttatcact taaattgaag gtattcttaa tatcagaagt agcttttcta atattaacct
     5221 tgtctttaaa tgtaatataa taataatcat atgtttctga ttttttgata gcttgtgtac
     5281 ctaatattga aagcattaat ccatgcttat ctgaggctct taagcttgat aatgatgaat
     5341 attcacctac tatcttataa tttttatttc taccatctgc taattttatt ttgataaaat
     5401 ctcctacttt tagattaaag cttttaactg catctacatc tattaaagtc tcattctcca
     5461 caacaggata acgacctttt attatttcta gacctaattg ttttgctaaa tcttcatcta
     5521 aaccttgtat caaaatctcc tttgatttaa actctccatt atcaatgact tgtcctctac
     5581 catagtgagc tatatctact ctactactta tttctgatat agcttcttca ttaagatttt
     5641 taagtactat gtgccaattt ccatcatctt ttataaattg tactttttga cattgtatag
     5701 ccatgtctgc tagtccaaaa atagcagtaa ctaaacatac tgcaatgaca atacataaaa
     5761 ttgtagttct attttttttc ttatgtacct ttgcatattg tggtactagt tctaaatagc
     5821 ttctcattct ctattcaccc ctaggtcttt aagcattcca tcagaaacct gtagtatcct
     5881 atctgctgta gaagctatat ttgtattatg agttatcata ataatagttt ggttatattt
     5941 ttctgctgtc acttttaaaa gtgaaattac atcactgcta tttttactgt caagatttcc
     6001 tgttggctca tctgccaata taattgatgg ctttgtaatc aatgctcttc caattgcaac
     6061 cctttgttgc tgaccaccag ataattgatt tggaagatga ctttttctat ctttaagacc
     6121 taaaattgtc aatacttcat ctagataaat agtgtctggt tttttgtaat ctagtagtaa
     6181 aggaaatatt atattttgtt ccacagttaa ttcgggtata agattatatg attgaaagat
     6241 aaaacctata ttacgacgtc gaaaaatagt ctgtttttct tctttcattg aacatatttc
     6301 tttaccatca acaaatattt ttcctgaggt tggagtatca agacctccta aaagatttaa
     6361 taatgtactc ttacctgagc cagattctcc aactacagta acaaactctc cctttgaaac
     6421 tgtaaatgat acatctttta atgcctctac ttttgtatat ccatttccat atgttttaca
     6481 taaattatta accacaagaa tatccatttg ctctatctcc tttctgatga ttaatataag
     6541 aataattcat acaccttact gtaagatgta tcctatctta catattagta agctttaaaa
     6601 aacttagagt aaatgtactt ccttgtaaaa aattactttc tacagttatg gttccattgt
     6661 gctgttctat gatagattta actagtggca accctaatcc aaggccttga ttactttttg
     6721 aaaacttact cctataaaat cttttaaata tatgatgtat atcttcaata tgaattccat
     6781 ttccggtatc actcactgaa ataatagttg ttataggtgt ttcaacccat tctatcatta
     6841 ttttatcgtt ctcttttgta ttatctaatg cattttttat aaggttgctt aaagcttctg
     6901 atatccattc ataatcgcaa aacaagtttg tattttctag acctcttata gagatactct
     6961 ttccttcttt ttctgctcta gtttcaaaac tatataaaat tttagtaatc aactttttta
     7021 cgttaacact agctttgtta agaattattg tctttgaatc tatctttgta atctttaaca
     7081 agttttgtat caatgattct atacgctcta attcattttg actcttctgc acaaaattct
     7141 caacaatttc actattatga ctctcttcct gcaatatttg attatacatc atcaatgctg
     7201 taagtggagt ttttagttga tgtgatatat ctgaaattgt atttttaaga aattctttat
     7261 tctgcttttc tttcaatata tgtgtgttta gagatgttga catagcatct attgaaccaa
     7321 ataactttga taaactacct tcttcataag tatttaaccg tacatttata tttccattca
     7381 taaatgaatc aattttataa ctagcttctt ctatcttgtt ctgttgctta aaaaaataca
     7441 catacaatat aaaaataatc atagtaaaga acacaaataa atacaaaaac atagttttta
     7501 tatatttacc ttgaaacaat ttcacacttg gcataaaact atcatctgta ccaaagtcat
     7561 atctagctgt atttaagatt tcttttcctt ttaaagtata tttatcttca ttagtagtaa
     7621 atactgctgg tatttttgat ttatcaacac ccatactatt taagtatcca gctatattat
     7681 aatcatgttc taacagtgca cttttataat cattagttat atttataact actatttgac
     7741 ctactactat aaacatcaat aaaacggata taatcaccaa aaaaaatatt tttatgtctt
     7801 tattagaaaa taccttcata ttaacaccct cactttataa cattccattt gtaacccatt
     7861 cttctaactg ttaatagaat ttttggattt tcaggattat cttcaatctt atttcttaac
     7921 cttcttacat atactgacag agtattatca tctacaaaac taccatttcc atcccataac
     7981 ttatccaata tattttttct tgtaagtaca atatttttat ttttcataag taagcataat
     8041 aatttatatt ctgctgtagt aagctcaagc tcaatttcat ttttaaaaac acgattttca
     8101 agtaatttaa cagtaatatt atttgatttt agttcagtta cattttcact tgtaaaatta
     8161 taacgtctaa gtaaagcttt tattcttgaa attaactcat ttaatttaaa aggttttgtt
     8221 atgtaatcat ctccacccat atcaagtccc atcactacat ttacttcttc atcagaagca
     8281 gttaaaaata tgattggtac attagaagct tctcttaccc ttttacaaat atcaaaacca
     8341 tcaccatcag gtaaggatac atccaaaagc agtaaatcat aatttttttc tttataaatt
     8401 tgcaatgctt cttctactgt tcttgctaca tcaacactaa atccattctt ttgtatagaa
     8461 tattctaatc catatatcaa acttaaatca tcttcaacca acaatatact actcatctca
     8521 atcacttcca tttttttatt ttttacttta tatatattat atacctaact tattatattt
     8581 ttactctttt aaaaatacaa taccttgaaa tcaatgattt aaatatttta aaattatttt
     8641 tatatgattt tatatagtta atttaatact ttgaaatctt aataataaaa aaagcttaaa
     8701 atacactaag tattctaagc tttattcaat cacattttat atatttatat tatttatttt
     8761 tttaaaatac tattttcaat attgcctcta ttaaacattt cattatcatt atctaaagag
     8821 atatcactta tatttctaac agttgttgaa tagttaccac taagttctat ctcactgaaa
     8881 tgcatatgca ctatcttttg tattacaaat cccattgatg caagtaaaat agcaaatatt
     8941 atatactgac taaagttact catataataa tttataatgg tatattcatt tcctttgact
     9001 gttaaatgtt ttgtagatag catagtatat atatattcag tacaatttat aaatacatat
     9061 ataccataaa tagctaacac tacagataaa caatacattg tgattgaaat ctttgatatt
     9121 tttttaaact tactatcttt acaacactta atatttgtta tttatcataa acaaggttat
     9181 tgtattatca ataattttta ggttacactt attattagtc ttgtgttaaa acgacagtag
     9241 acttaagagt agagttttat taagccaata tttatatttg ctatacattg tacccctatt
     9301 acaccataca tataacttgg ataacttatt ttatttttat ttctaaatta tttaaaatca
     9361 atatctacac cattatcgat aattgcaatt aatttcatat aaaacctctt taat
//
LOCUS       DAELNY010000146         9354 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.148, whole genome shotgun sequence.
ACCESSION   DAELNY010000146 DAELNY010000000
VERSION     DAELNY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.148"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            888..1262
                     /locus_tag="KPE55_002862"
     CDS             888..1262
                     /locus_tag="KPE55_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0493907.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            1264..2121
                     /locus_tag="KPE55_002863"
     CDS             1264..2121
                     /locus_tag="KPE55_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493908.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            2124..2762
                     /locus_tag="KPE55_002864"
     CDS             2124..2762
                     /locus_tag="KPE55_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0493909.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            3768..4502
                     /locus_tag="KPE55_002865"
     CDS             3768..4502
                     /locus_tag="KPE55_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0493910.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            5920..7176
                     /locus_tag="KPE55_002866"
     CDS             5920..7176
                     /locus_tag="KPE55_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0493911.1"
                     /translation="MIEKFKTLIWIKMIIKIKSVYKTYGGLFLLGLIGIPIGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIILGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            7239..8432
                     /locus_tag="KPE55_002867"
     CDS             7239..8432
                     /locus_tag="KPE55_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0493912.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIVGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKIHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            8633..9013
                     /locus_tag="KPE55_002868"
     CDS             8633..9013
                     /locus_tag="KPE55_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0493913.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         3485 a          980 c         1511 g         3378 t
ORIGIN      
        1 aaaattaaaa agaggagaaa aaagggagtg ccacaaagtg aactttttag ttctaaggca
       61 ctcttttttg tacgaaatcg aatatatttt caacgtttta aaagtgagaa agagacttat
      121 ctctagtttg agatagccaa ttttaattat taaaattatt ttttgtttta tttatcaaag
      181 agtatccgtg ttttagaagt tagtattttt tattttatat atacaaggtt ttccgtttct
      241 aatgttttcc tttttaaaat aattagggta ttcaattata gtaaaaggtg gatggtattt
      301 tggagtgata ttatttaaag cacagtattg aatgttgaaa tcctcacatt ttagagtatt
      361 atcttttgta atcaaaaaaa tcttctatac ttatagcaag gttatttctg caaatattcc
      421 atacataata atatcccaat ttaaattttt tttatataga gtttacctct agtctacact
      481 cttgaataac tctttcctct atccaaattc ttataaattc atcccattta ttactatcta
      541 tttattacta tctaaatttt ttagtatgtt tgtaattaga aatggattta ttcctttatg
      601 ttttttataa atagtttata acttaattta tcatcaaaca aatcattcat acaggattac
      661 tttttctttt agatattcaa ttcttacttt taataaatat ttaatacatt ctatcttatg
      721 tggttttaaa aagtgtttat ggctattaac aatctctaat atttcattca tatttatttt
      781 gtcatagtaa tattttacat cttttatatt gacaaaaaag tgctctaagt gtatagtgtg
      841 tatataacaa catcacactg aagatgataa aaagggggtg ataaaaaatg aaagtgctca
      901 tttctaacac atcagataag ccattatacc aacaaattaa agagcaaatt aaagaagcaa
      961 tatttactga agaactgaaa gaaggggatg caatgccatc cataagaaat tttgctaatg
     1021 atttaaaagt tagtattttg actattagac gagtgtatga agaacttgaa aatgaaggct
     1081 ttcttacacg ccaagttgga ataggaacat ttatttcaac tggaaattta gaaatactaa
     1141 gagactcaaa gagacgtata gttgaacaaa aaatggcaga tatgattaag gatgcaaaag
     1201 cactaggaat tacacagata gagttacaag aaatgatgga tattctatat caggaggatt
     1261 aatatggaaa atattttaga agtttataac ttaaataaga cttataccga tttttcatta
     1321 tctaatataa gctttgcatt accagaagat tgtataactg gatttatagg tgttaatgga
     1381 gcaggaaaaa caacaacaat ccgttcgata ttggatgtcg taaagcgtga tagtggaaaa
     1441 ataaaaatct ttggtaaaga tttcgatgga aatgagaagg aattaaaaaa tcgaattgga
     1501 gttgtatttg atgattcata tttctatgag gagttgtcaa tggcagaaat gaaaagtatt
     1561 attgcacctt catatgataa ttggtgtgaa gaagattatc aacaaaatat gaagcgtttt
     1621 aatcttaacc caaaacaaaa gattgataca ctttcaaaag gaatgaaaat gaaatttgct
     1681 ttagcattag cattatcgca taaagctgag tttttaatta tggatgagcc tacaagtggc
     1741 ttagacccac taatccgtag tcagatactt gacatactaa cagaatatat gaagaaatgt
     1801 ggtcgtggtg ttttcttttc cactcatata acatctgatt tagacaaaat agcagatatg
     1861 attgttatga ttgacaatgg acgtattata ttagagcgtg aaaaagatac tctattggac
     1921 gaacatagaa aagtaaaagg agatattcaa tttctaaatg atgaaaccaa aaaattattt
     1981 ttaaatttag aaattaccgg ctatggtttt atagggatta cctcaaaaat taatgaagtg
     2041 caaaaaaata tgccagatat tttaatagag cgtcctacaa ttgaagatat tatgcttgca
     2101 tatattgaaa ggagagagta attatgttga ttcatttagt taaaaaagat attctgattg
     2161 taaaaaaata tgtattgttg atattattta ttgcaatagg aatcccatta tttatattgt
     2221 ggcgtttacc tccatttgca caaatattgg gatttgtact atccattata ttttcagaat
     2281 ttatgctgtg ccagtatcta ttattaaagg agagtcaata ttcaaaagca tctgtattac
     2341 tttgttcaac tccttatcct agacgtggct tagttcaatc taagtatgtt ttatttgcaa
     2401 tggtatttat ctattgtaca ttagtttact ggattgaaaa tttacttatt ccacaaatag
     2461 gaacttttga cttaacatat attttgattg ttttattaat aagttctatt atttatggag
     2521 tttatatgcc tattcaatat aaattaggat atgaaacaac aaaattcttt ttcgtggtga
     2581 taatcatggc ttcatcattt gtattaccat taactatagc tcacagcaat tttgaaattc
     2641 catttatata tcagtatcca tccatgatag tgaatattgt attgttcctt ttaagtttaa
     2701 ttatactgtt agcttcaatg tttatttcta ttaaaatttt tagtaataag gagcttgtat
     2761 aaggtaaggg ggacttttat atgccacatt taccagattg ggctgtttta ctttgtatct
     2821 tacttccaat agtatataaa gcttgtcaga tatttaaaag taaaaaatag agtatatatt
     2881 tatattgcca tttttattga gtgaagttag atggtatgaa aaggttactt gaaaataaag
     2941 ttacttggtg tgggagagta acatcataat attatagatt atatgtttct tgtgaatcaa
     3001 tttgtatttt ttaataggga atatccagta attttgtttt ccattgatta tttttcaatt
     3061 atgagtatga ttgtgtgtat tgtataccat cttttaaggt gtttttaaaa aattatatcg
     3121 tcaaaatata aatctaagga atcctataaa taaagctttt taattttaga aaactaataa
     3181 aaagtaggac gttaataaaa agaagctgtc tcaaaataga gataagcttc tttttcattg
     3241 ctgaaatgac gaaaatataa ttgatttaat ataaaaaaga gtgctttatg aactatttat
     3301 tttgaacagt agatacactt gcactcaatt tagttactcc taagctttat gaactaaaag
     3361 tttattttga agcttatttt tattaaaaga gattttattt ttctaaataa tcatctatat
     3421 acagtattgc gtttctttta tttattcata atttattttc ttattaaagt ttttctatat
     3481 tcatattttc tatattactt tacttttaat tttatacgaa ttatcaaaaa taaaatagtt
     3541 attagaaaaa aataaaagta attgcaaagc ttgcaattac ttacttctat attaattact
     3601 ataccataaa ataacaaaaa aatcaagtcg agtaatcctt caacttaaat gatatactag
     3661 gtattaataa tttttaatta ataaaatata actagaggaa aatctacatt ttctcaattt
     3721 ctttaattaa attgacctct aattaagtaa ttataaggag aaaaattatg aatttagcaa
     3781 agggattaga tgttttaaaa atttcatcaa atgtattcgg tgaagataaa gttatgtata
     3841 ttcctgtaat ctacacagaa gatgatgcaa cacttattga cacaggtctt ccgggtcaag
     3901 gtgatttaat aattgatgct cttaataaaa gtaacacgag ctttgatagg ttaaaaaata
     3961 taataattac tcatcatgat atagaccata taggaaacat aaattatcta agagaaaaat
     4021 ctaagaataa tatcaaagta tatgcataca aaagtgaaat cagctatata acaggagaag
     4081 aaactccttt taaactttat atgttggaac aaatggtaga taaaattgat gataagatgc
     4141 ttagtatgct aaatgttatg ggacttggat ttaaatcatc atatactaaa gtagatgtgt
     4201 cattagataa ccatgaaaaa ttgaatctag gtgaggaaat agaagttata catacaggag
     4261 gtcacactag aggtcacata tgtctttatt taaaagagtc taaagtactt attgctggag
     4321 atttgttaca ggtagaaaat ggagaactta agcctgttga tgtgatgtac agcaacaaac
     4381 aagagttaaa agatgctatt aaaaatataa gtaattatga tatagaaacg atagttttta
     4441 gtcatggtgg attatatcaa agaaacataa ttggaacatt gaaaaaccta attattgagt
     4501 agtactatat gatttacaat aatttcttaa attacttaaa attatatcta aagatgtaga
     4561 atcaaaatta aatataagta ataagaattt tgctttaaaa ttatacttaa gtgttaagga
     4621 tgatacgtag tattacaagt attttagaat ataatttttt atagtaattt taaggttcta
     4681 caatacttca gttgaaaaat atttaacagc atagtataat aggaaagaaa atactttaaa
     4741 taaactttag catacaaagt gaagaaaaat atttaaatta tatataatat tgtagagtga
     4801 ggtaaaatat ttaagttata tatcataatg cagagtgaag aaaaatattt agataatgta
     4861 ccataataca aagtgagtaa aaatatttaa atcatgtatc ataatgcaaa gcgaagaaaa
     4921 atatttaaat aatgtaccat aatgtagagt gagtaaaaat atttaaataa tgtgttataa
     4981 tacaaagcga ataagattat ttaaagcatg tgttataata caaagcgaat aagattattt
     5041 aaagcatgta ttataatata aagtgagtaa aaatatttaa atcatgtatc ataatacaaa
     5101 gtaaagaaga ttatttaaac catatatcat aatgcaaagt gaagaaaaat gtttaaatta
     5161 tgtatcataa tataagcaga gtgaagaaaa atatttaaat aatatatcat aatgtagagt
     5221 aaagaaaaaa tttaaatcat gtatcataat ataagcagag tgaagaaaaa atttaaatca
     5281 tgcatcataa tataagtaga gtgaagaaaa atatttaaat aatatatcat aatgtagagt
     5341 gaagaaaaat gtttaaatta tgtatcataa tataagcaga gtaagtaaaa atatttaaat
     5401 catgtatcat aatgaaaagt aaagaaaatc atttaaatta tatatcataa aaataatatg
     5461 aaatattgaa cactcataat attagagact ttaatatttt aattgatagg ttatattatt
     5521 tataacatta aaaatacaat ttacataaaa tagacatatg aaaaataaaa aattatataa
     5581 taattatggg aatgaggttc tcccttggat gataatatcc taaactgcca atgagctgat
     5641 gacttctatg atatcataga tgccaccagc tttttttgtt gtttaaatcg ataaaatgga
     5701 ggtgaattac ttactaaatt tttatttttt aaatttgaag aaatatttaa tcaataaaaa
     5761 aaatagtaga ttatatgtta attattttat taagaatggg ttaagagtgt acctatatcc
     5821 tatcaattaa aaaaacaatt acgatagtca ttacattgac tacagttgat gcaataaaac
     5881 tttcaaaaaa tcttatgatg caaatagaaa ggaattaaaa tgatagaaaa atttaaaact
     5941 ttaatatgga ttaaaatgat aataaaaatt aaaagcgttt ataaaacata tggtggacta
     6001 tttttgttag gactcatagg tataccaatt ggtgcaatta taggtcttat agacaccata
     6061 tttggaacgg ttcttctaaa agttacagat attcgagaaa catatccaat gtatctgata
     6121 ccatttttag cagtagtagg agttgttatt gcttattgct attttaaatt tggaggaaaa
     6181 agtagtaaag gaatgaatct aatatttgag gtagggcatg gagaagaaga aattattcct
     6241 ttaagattgg ttccatttat aatattaggt acatggttaa cccatttatt tggtggtagt
     6301 gcaggacgag aaggagttgc tgttcagatt ggtgcaactt tttcacattg ggtaggtaaa
     6361 aggttgccta taaaaaatgc ttctagtatt tttctagtaa ctggtatggc agctggtttt
     6421 gcaggattat ttgagacacc aatagcagca atactatttg ctatggaggt tttggtagct
     6481 ggttcactgg agtatcaatc gctttttcct gcgtttactg cttcttttac agcaagtgca
     6541 gtatcaaaag ctttagggtt agagaaattt tcttttgcac tttcttctaa agttgtattt
     6601 gacttgtcaa tattttggaa gcttattgtt ttaggaatta tttttggaat ggttggtgga
     6661 gcatttgcat ggtgtctgaa attgtcaaaa agaaaaatag ggaatagatt aaaaaatcct
     6721 atgataagaa ttgctattat tggagtatgt cttagtgtat tattcctttt attttataaa
     6781 ggaagatatt caggtttagg gacaaattta attcaaaata gtttctatgg tggagaaatt
     6841 tattcatttg actggctgtt aaagtttata cttacaatac tgactttgtc agcaggtttt
     6901 caaggaggag aggttacacc tttgttttcc attggagcta gtcttggtgt attgttagca
     6961 ggatttttta atttgccaat tgaattagtt gcagcgcttg gctatgctag tgtctttggt
     7021 agtgctacaa atactttttt tgcacctgta tttatagggg cagaagtttt tggatacagc
     7081 tatttacctt atttctttgt agtatgcgct atttcatata tatttaatat ggataaatct
     7141 atttattcac tacaaaaaat ttcaacaaaa caataaataa aacaacataa tgtatgatta
     7201 gaagagaaag aatattagag aaaggatagg gtaggaatat gttgacaagt ttagctttga
     7261 tttttttatt aggaatggcc tcgggaggta tttttaagag gataaagtta cctaacttat
     7321 tgggaatgtt acttacagga attatattag gtccatatgt tttaaatttg attgataatt
     7381 caatactaga tatttcttct gaccttagaa agattgcatt gattattata ttaacaaggg
     7441 ctggattatc tttagatata aacgatttga agaaagtggg aagacctgct gtacttatgt
     7501 gctttattcc agctactttt gaaattgtcg gaatgatagt tttagcacca aagttactag
     7561 gcgtttctat attggaagca gcagtcatgg gtgcagtagt aggagcagtt tctcccgcaa
     7621 ttattgtacc taaaatgctt aaattaatgg aagaaggtta tggtacagaa aaaagtattc
     7681 cacagatgct tttagcagga acttctatag acgatatttt cgtaattgtg atgtttacag
     7741 tattcacagg attggcacaa ggaaatagta tatcagctat tagtttttta caaattccag
     7801 tatcaattat attaggtgta atagttggag cagtgattgg aatctgttta gcagtatttt
     7861 ttaagaagat tcatatgaga gattctgcta aaggagtgtt gcttttaagt atctcatttt
     7921 taatgataag tttagagact gctttagagg gtattgtgcc attttctgga ttattagcag
     7981 ttatgagtat tgggattttc cttcagataa aatatcgtgt agttgcaaga agattgtcta
     8041 taaagtattc gaaactatgg gttggtgctg aaattctctt gtttgttttg gtaggggcaa
     8101 cagtagatat aagttatgca tttaaagcag gaattggagc tgtaatttta atatttggag
     8161 ttcttttatt ccgtatggta ggagtattct tctgccttat taagacaaat ttgacaataa
     8221 aggaaagatt gttctgtatg attggctata ttccaaaggc tacagtacag gcagcaatag
     8281 gaggtgtacc acttgcaatg gggatggcat ctggacaact tattttgacg ttagcagttc
     8341 tagctatact tataactgca cctcttggag cttttggaat tgatgtaact tataaaaaat
     8401 tattaacttc tgtaaaaggc gagaataagt aaaaataaag attattttaa ctggtaagtt
     8461 atattttgga aaaggattat actattgaat acaatataaa aaaatcattt gggagattaa
     8521 gaaggtagaa gttaagaaca gattttaagg tatgttatga ttattagaat tgattttgtg
     8581 aaattattat tgtaatattt aattatatag ataaagaagc ggagtgataa ctatgtgttt
     8641 aaaaaaatta tcaaaattag agttagttat tatgaaattt atttggaatt tagatataaa
     8701 aaccaattca tatgagatta ttgattatat gaaagaagaa cataatttac cagaaaaagt
     8761 agctttaaaa actttatcaa aactatcaaa gaaaagattc ttatatgttc aagaaacagg
     8821 caagtgtatg tattatacag ttgcaataaa agaaaaggca tatttagagt ttatatcaag
     8881 aaatgtgcaa aatcttttga agaataattt tataaggaat ttattagtat catttcacga
     8941 agaggagcta acagaggaaa agataaagtc gttagaaaac tgggtagtaa attgggaaga
     9001 agcttatgta taaatgttat tatttttatt acaatttaga taattttggt tttgtaagct
     9061 ctgcaactat ttttagatgg ttgcagagtt tatttttatt ttttattccc ttaagcaata
     9121 cttaaaagta aacgcattta tatttactaa cttcaattac ttataatact taaattactt
     9181 tttaaaaata ttttgatatg tcatctctat agtaatctag aacttgtttc tattctttat
     9241 ataaagtttt tatgaatgat atatataata ttcgttcatt tgattaaaaa taacaaaata
     9301 ttaaataatt ctactctgat agtttgttaa aaaaataata aaaaatatta ataa
//
LOCUS       DAELNY010000147         9234 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.149, whole genome shotgun sequence.
ACCESSION   DAELNY010000147 DAELNY010000000
VERSION     DAELNY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.149"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2217
                     /gene="smc"
                     /locus_tag="KPE55_002869"
     CDS             <1..2217
                     /gene="smc"
                     /locus_tag="KPE55_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG0493914.1"
                     /translation="QNENLKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYN
                     SKLNVYIDMENHYEGFNRGVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYM
                     QNIITDNEFSAKFAINYLKQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLI
                     TFDEKYRNIIENILGRTILINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSL
                     KTNGNILSRKRYINEYTEKISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDL
                     EKSIIIKSTSIKNVESEIESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDD
                     LYNKNKEKIDALNEEIKRYNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGEN
                     CELEEKNKQLEESLNYEEHEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDD
                     LKNSFDEINKELKTIDRQHIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQA
                     LEIKDEDLDIDRKFLESLKREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESME
                     EIEKLIHTLEENMKSEFEIKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILI
                     TAQPPGKKMKNLNLLSGGEKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFG
                     EFLKDLSKETQFISVTHRRGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            2244..3524
                     /gene="ftsY"
                     /locus_tag="KPE55_002870"
     CDS             2244..3524
                     /gene="ftsY"
                     /locus_tag="KPE55_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG0493915.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            3627..3995
                     /locus_tag="KPE55_002871"
     CDS             3627..3995
                     /locus_tag="KPE55_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG0493916.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            3992..5344
                     /gene="ffh"
                     /locus_tag="KPE55_002872"
     CDS             3992..5344
                     /gene="ffh"
                     /locus_tag="KPE55_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG0493917.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            5382..5654
                     /gene="rpsP"
                     /locus_tag="KPE55_002873"
     CDS             5382..5654
                     /gene="rpsP"
                     /locus_tag="KPE55_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG0493918.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            5719..5946
                     /locus_tag="KPE55_002874"
     CDS             5719..5946
                     /locus_tag="KPE55_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG0493919.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            6054..6569
                     /gene="rimM"
                     /locus_tag="KPE55_002875"
     CDS             6054..6569
                     /gene="rimM"
                     /locus_tag="KPE55_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG0493920.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            6578..7273
                     /gene="trmD"
                     /locus_tag="KPE55_002876"
     CDS             6578..7273
                     /gene="trmD"
                     /locus_tag="KPE55_002876"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG0493921.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            7458..7808
                     /gene="rplS"
                     /locus_tag="KPE55_002877"
     CDS             7458..7808
                     /gene="rplS"
                     /locus_tag="KPE55_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG0493922.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            8129..9025
                     /gene="ylqF"
                     /locus_tag="KPE55_002878"
     CDS             8129..9025
                     /gene="ylqF"
                     /locus_tag="KPE55_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG0493923.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT         4014 a          883 c         1772 g         2565 t
ORIGIN      
        1 caaaatgaaa atttaaagaa tgtaaataat agacataaag aattatcaat taatttgcag
       61 gattcaatta gtgaacacaa taaactagaa gatgaaatac agaaaagtaa atataattta
      121 aatggatata attcaaagtt aaatgtctat atagatatgg aaaatcatta tgaaggattt
      181 aatagaggag taaaagaagt actaaaaaat aaaaatttaa aaggagttca tggagcatta
      241 ggacagatta taaatgtacc tgaaaagtat gagaaatcta tagaagcagc tcttggtgct
      301 tatatgcaaa atataataac tgataatgag tttagtgcta agtttgcaat aaactatcta
      361 aaacagaata atttaggtag agtaacattt cttccattaa atataattaa atcaaataaa
      421 ataagtctag gaaatttaaa agcaaatact aagttcatag gaatagcaag tgatttaatt
      481 acatttgatg aaaagtatag aaatataatt gaaaatatac ttggaagaac aatacttata
      541 aataatatag atgagggcat aaaatttgca aaagaaactg gtcataggtt taaaatagta
      601 actttagatg gtgaaatctt aaatccagga ggttctttga ctgggggaag tctaaaaact
      661 aatgggaata tactatcgag aaaaagatac attaatgaat atacagaaaa gataagtaat
      721 atcaagaatg aaatttcaca tctagaatta aaaagagaat ctttagacaa agatgtgaaa
      781 aatatcaaaa atgaaattga ttcacatgaa agtaaaataa aagatttaga aaaaagtata
      841 ataataaaaa gtacaagtat aaagaatgtt gagtctgaaa ttgaatcgtt aaaaggtagt
      901 attactaaat tagaaaatga gaagaatgat ttaaattcta acctaaatta tacacttgaa
      961 aaaagtgatg atgttagaaa agatatggaa gagttagatg atttatacaa taaaaataaa
     1021 gaaaaaattg atgcattgaa tgaggaaata aaaagataca atgatttata tgataaagaa
     1081 aaatctgaat ttgatgaatt gaacttaagt ttagtgaaga aaacagaagt ttacaatagt
     1141 atagttagag atataaaaag aatatctggt gaaaattgtg agctagaaga aaaaaataaa
     1201 caattagaag agtcattaaa ttatgaagaa catgaaataa ttaaattaca agattctata
     1261 ctcactgaag aaaaagaaaa agaaaattta acaaaacaac taggggatag taatagaaat
     1321 cttgaaacta gaaaaatagc aaaagatgat ttaaaaaata gttttgatga aattaataaa
     1381 gaattaaaaa ctatagatag acagcatata gaacttaaag aaagtttatt taaagttggt
     1441 ggaagattag aaagattaaa aactagtcaa gacacatata taaataagtt gtttgagcaa
     1501 tatgatatga ccttagtaca agcattggaa attaaagatg aagatttgga tatagataga
     1561 aaattcttag aaagtctaaa gagagaaata agaagtcttg gaaatataaa tatagactct
     1621 ataaaggaat atgaggaaat aaaagaaaga tacgattttt atagcgaaca aaagcaagat
     1681 ttagaagagt ctatggaaga gatagaaaaa cttatacata ctttagaaga aaatatgaaa
     1741 tctgaatttg aaattaagtt tgaagaaata agtaaaaatt ttaaatatgt atataagaga
     1801 ttatttggtg gaggttgtgg agaattaact attttagata aagaaaattt attagaaagt
     1861 gatatactaa taacagctca acccccaggt aaaaaaatga aaaatttaaa cctattatct
     1921 ggtggagaga aagctttaac ggcaataagt atattatttg ctatattgat tacaaagcca
     1981 acaccatttt gtatattaga tgagattgaa gcgccacttg atgacgcaaa tatatttaga
     2041 tttggagaat ttttgaagga tttatctaaa gagactcaat ttatatcagt aactcatagg
     2101 agaggaacaa tggaagctgc tgattatatc tacggagtta caatgcaaga aaaagccatt
     2161 tcaaaagtta ttagtttaaa attaaaagaa gctcaggaaa taacagatat tatataactt
     2221 agattgacat aggaggaata acagtgttaa aaaaattatt tggttttgga aaagacaagg
     2281 aaaaggaaat agaaaaaaaa gatgctgaag aagaaataga agttgaagac agtgttgata
     2341 atttagaaaa cttggaagaa actatttttt caggacttga agaagaagtg atagataaag
     2401 tggaagatat tgaagaaaaa aatgaggaaa gtgtcaataa tgaaggcact gaagaaatcg
     2461 aaaactttga aaaaatagaa gttgagatgg atagtaatgg aaaagaagta gaaaatattt
     2521 caaataatga ttctcaagaa gagataatcg aagaattaga aaattatgat gaaattgagt
     2581 ctaaagagat agaagataaa gaagataaga aagttaactt gtttgaaaga ctaaaacaag
     2641 gattgacaaa agcaaagcaa ggaataactg atagaataga tgaagtttta aaatcatata
     2701 caaaaataga tgaagaatta cttgaagatt tagaggaaat tttaataaca gcagatgttg
     2761 gtgtaaatac aactatggat ataattgaaa gactaagaga taaaataaag caaaaaggta
     2821 ttactgaacc aataaaggta agagaagaac ttaaatctat agttgaagat attcttacaa
     2881 atgaaaactc tacactagat atagaaccag caccatgtat aatattaatg gtgggtgtaa
     2941 atggggtcgg taagactact acaataggta aactagcaaa tagatacaaa aaagatggta
     3001 aaaaagtttt attagcagca gcagatacat ttagagcagc agcgacagag caattagaaa
     3061 tttgggcaaa tagaaccaat gtagacataa taaaacatca agagggagca gaccctggag
     3121 ctgtagtatt tgatgctata aaagctgcaa aagctagaaa aacagatgta ttaatatgtg
     3181 atacagcagg aagattacac aataaggcta atcttatgaa tgaacttgga aaagtattta
     3241 aaatagtaga tagagaattt ccagaagcaa aacgtgaagt acttcttgtg gtggatgcta
     3301 caacaggtca aaatgcagtt gttcaagcaa agacatttaa agaagttgca gatataacag
     3361 gtatagtact tacaaaactt gatggaactg caaaaggtgg agtagtcctt gcagtaaaat
     3421 cagaagtaga tgtacctgta aaacttattg gagttggaga aagtgtagaa gacctacaag
     3481 atttcaatgc aaaatcattt tcagatgcat tatttggtaa ctaatgttct tttaaaaata
     3541 atggttgaca agagtgttgt tatgatataa aatacaatag tgtaaagtaa ataaccttta
     3601 cagtatactg ctaggaagtg taagatatga atatagaaaa aatggtcgaa attggattgt
     3661 tatttgaaca gtataaagaa ttattaactg acaagcagaa agaaatagtt gctctatact
     3721 atgaagaaga ttattcttta ggtgaaatta gtgaaaatct gaatgtatca agacaaggtg
     3781 tttatgatac cttaaagcgt tcagaaaaaa tactaagaga ttatgaagaa aagttacact
     3841 tagtatcaaa gattcaagag caggaaaaaa atattaagat tataaaagat aaaattattg
     3901 acattaaaga agatttattg cacaatagag attgtgctaa tttaatccct aagctagaaa
     3961 atatagaaga tgtatgtagg gagatgataa aatgatattt gaaggattgt ctgataaact
     4021 acaaggggca tttagtaagt taaaatctaa aggaaaactt acagaagtag atgtaaaaaa
     4081 tgctatgaga gaagttaaac ttgctcttct tgaggcagat gttaacttta aagtagttaa
     4141 agatttcata aaaaaagttc aagagagatg tgttgggcaa gaagttatgc aaagcttaac
     4201 tccagctcag catgtaatca aaattgtaaa tgaagagctt acaagtttaa tgggagatgt
     4261 acaaagtaaa gtaatgattt cctcaaagcc tcctacaatt ttaatgatgg ttggattgca
     4321 aggtgcaggt aagactacta cagcaggaaa acttggagga tattttaaaa agcaaggtaa
     4381 aaaacctcta ttagtagctt gtgatattta tagaccagca gctataaagc aacttcaagt
     4441 tgttggagaa aaattggata tacctgtatt caatatggga gataaagaaa atccaataaa
     4501 tattgcgaaa gcaggtttaa gccatgcgat taaaaatgct aatgatttag ttattataga
     4561 tacagctggt agacttcata tagatgaagt acttatggat gagttaaagt ctattaaatc
     4621 agaagttaag ccacatgaga tacttttggt tgtagattct atgacaggtc aagatgctgt
     4681 aaatgttgca gaaagcttca atgaggcact tggtgttgat ggagtagtat taactaaatt
     4741 ggatggagat actagaggtg gtgctgcact ttcaataaga gcagttacac aaaagcctat
     4801 aaaatttatg gggatgggag aaaagctaga tgatatagaa cctttccacc cagatagaat
     4861 ggcatcaaga atattaggta tgggagatgt cttaagttta atagaaaaag cacaagaaaa
     4921 tatagactta gagaaagcta aagaattaga gcaaaaaatt aaaaaacaag agcttgattt
     4981 tgaagatttc ttacaacaaa tggaacaaat acaaaatatg ggacctcttg acaagatact
     5041 tggtatgata cctggtatgg gaaatgtaaa agaccaactt ggagatgtag atttgaatgg
     5101 caaagaaatg aagagaacaa aagccataat tcagtcaatg actacatatg agagaagaaa
     5161 cccaggagta ttaaatgctt caagaaagaa aagaatagct aaaggcagtg gaactagtgt
     5221 acaagatgta aatagactta taaaacaact taatgaaatg aaaaaaatga tgaagatgtt
     5281 tgctggttca caaaagagta tgaagaaaag gggcggctta ggcggtttac ctttttttaa
     5341 ataaaatata aaaaagtaaa aattattcgg aggtgactgt aatggcagtt aaaataagat
     5401 taaaaagaat gggagctaac aaaaaacctt tctacagaat agtagtagct gattcaagag
     5461 ctccaagaga tggaaaattt atagaagaaa taggatacta taatccaatt tctgaaccta
     5521 aacaagtaag aataaatgat gaaaaagcta taaaatggtt agctactggt gctcagccaa
     5581 cagaagtagt taaaaaatta cttgtaaaaa acggagtaat agaaaaattc gaagcttcta
     5641 aacaagcaaa ataatttttt cttggcaatt agtttaagta agggctcatt gctaaggtta
     5701 ctttaggaga tgaaatatat gaaagagcta gttgtagata tagctaaggc tctagttgat
     5761 aatcctgact cagttgttgt tgaggaattt gaagataatg atggtatcgt cttaaagctt
     5821 acagttgctc aagaggatat gggtaaggtt attgggaagc agggaagaat agctaaagct
     5881 ataagaactg tcgtaagatc tgttgcaaat agagaaaata taaaagtttc tcttgagata
     5941 gtataaaata ggaggattag gtataatgcc tagtcctttt tatataaatg cgtagtttaa
     6001 gattttatga tgatatgacc acataagatt aaatttttta agaaggtgat aacatgaaag
     6061 aaaaattaac tcattttaag gtaggtcaaa tagtaaatac acaaggttta aaaggtgaag
     6121 taagagttta tccgcttact gatgatatag agaggtttga tgaattaaaa gatttctatc
     6181 tgggaaaaga tttaaataat aaattggctg ttgaaaaagt aagatacaaa ggttctatgg
     6241 ttataatgaa gataaaaggc atagacagca tagagaaagc agaaaaacta aaaaataaat
     6301 ttatgtatgt agctagagaa gatagcagag agcttgaaga aggcgaattt tttatagcag
     6361 atatgattgg aatggaagtt ttaactgtag atggaaaata tgtaggaata ctagaagatg
     6421 ttcttcaata ctcagcaaat gatgtctatg tagtaaaagg tgaggaagat aaagaattta
     6481 tgataccagc tataaaaaaa tttgtaccta caatagatat tgatgaaaga aaaatgatta
     6541 tagaccctat taagggaatg atagattagg tggtaatatg agatttcata taatgacact
     6601 ttttccagaa atatttaatt cgtacatgga tgaaagtata atgaagagag cagttgaaaa
     6661 aggtataatt gaggttcata tatataatat aagggatttt tctaataata aacataaaaa
     6721 agtagatgat tatccatttg gaggaggagc ggggatggta atgactcctc aacctatata
     6781 tgacacatat aagcatataa taacaactca taatataaat aacccaagtg taatttatct
     6841 tactccaaaa ggtaaggtat acaatcaatc tatggcaaag cagatgtctt taaaagaaga
     6901 tataatacta ctatgtggtc attatgaagg aatagatgag agaatcattg atttaatagt
     6961 tactgatgaa atttcaattg gagattatgt attaactggg ggagagctcc cagcacttat
     7021 aatgatagat tctatatcga gattaatacc aggtgtatta aaccaagagg aatcatttga
     7081 agaagaatca tttaaagata acttattgga atatcctcat tacactagac ctagagattt
     7141 tgaaggatta aaagtaccag aagtgctttt atcagggaat cataagaaga tagatgagtg
     7201 gagaagagaa gaatctataa ggataactaa agaaagacga tttgatttat ataaaaagtc
     7261 taatgaaaaa taaaaatata atgaaaaatt aatatatatg tatacaataa agtacctata
     7321 aatggaatta aaaacataaa agtgcttgta ataactattg aagtatagta taatataaaa
     7381 tgttgaaaat acgggcgttc ctctgtttac actggtcggg tagttaagaa cgtctatgat
     7441 atctaggagg aagaaagatg aatgaaatgc taagagcaat tgagcaagaa caattaaaaa
     7501 atgaagttcc taattttgga cctggggaca cagttaaagt acacgttaga attatagaag
     7561 gaaaaagaga aagaatacaa gtattcgaag gtgtagtatt aaagagacaa ggtggaggag
     7621 caagagaaac tttcacagtt agaaaaatgt ctttcaatgt tggtgtagaa agaactttcc
     7681 cagttcactc tccaaaaata gaaaaaatag aagttactag aaaaggtaaa gtaagaagag
     7741 ctaaactaaa ctacttaaga ggtagagtag gtaaagctgc taagataaaa gaagctagaa
     7801 ataaataatt aaaaacttta cttataaatg gggggctgat ttatcagtcc tctttctatt
     7861 ttaatgaaaa ttacagcaaa taattatact agaaataaat agaaatattt gttgaaaata
     7921 tacattattt atctatgaat ttatagcctt taaaatgaaa aataaaattg gtaaatattg
     7981 cattatattt tgtaacaata ttaaaaataa gagaacagta ttatgtaaag tatgacgtta
     8041 aaaagatgac aatattatag tttgttaaga tagatttttt aagtggaatt aggatataat
     8101 aatataatat taataaggag tgatacatgt gtcaaatgaa tacgaagagt atcttatgaa
     8161 tgacaatcta catataaact ggtatccagg tcatatgaaa aaaactaagg agttagtaaa
     8221 aaataactta aaattgattg atgtagtaat tgaacttttg gatgctagaa tcccgtttag
     8281 tagtaaaaat ccagacatag acaggcttgt aggagataag ccaagagttg tcgtacttaa
     8341 taagagtgat atggcagata gggataaatt gaatcaatgg attgaatact ataagaaaat
     8401 taatatcaaa gctataccag tagatacaat aaaaggcgtt ggtataaata agataatcga
     8461 agaatgtaaa aatgtaacta gagaaaaaat gagttcttta aaagacaaag ggagaaaaga
     8521 aagagctata cgaataatga tagttggagt tccaaacgtt ggtaaatcgt ctcttataaa
     8581 caaattaaca ggtagaaaaa gcactcaaac tggagataag ccaggagtaa cgaaagggaa
     8641 acaatgggtt agattaaagg gaaatcttga acttttagat acacctggaa tactttggcc
     8701 taagtttgaa gaccaagaag ttgcattaaa tttagcattt agtagagcta taaaggatga
     8761 gattttagat actgaaactt tggctttaag acttatagag aaacttatga aaatagaacc
     8821 tgagaagttg aaagccagat ataagttaga ttgtttagga gaaactccaa ttgaaactat
     8881 ggatatgatt ggccataaaa gagggtttat aacaggtaaa aaagaactag attatacacg
     8941 catagccaca actgtattaa atgaatttag agatggaaaa ataggaaata taacattgga
     9001 agttccagaa aacgttaaaa gatagatttt aaaacaaaat caaaaattta atgttaaaaa
     9061 tttaaaaaag ccttttagca aaggcttttt tatttttttg aaatatagac aaatatctcc
     9121 atataaaagt ataaattagg ttagaaaacc tttaaaaata ttaatttaag acaaatatac
     9181 ctaatatttt gaaaattaaa atgagtgtga ttatttaaaa ttttagcata ttga
//
LOCUS       DAELNY010000148         9177 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.150, whole genome shotgun sequence.
ACCESSION   DAELNY010000148 DAELNY010000000
VERSION     DAELNY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.150"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            628..1277
                     /locus_tag="KPE55_002879"
                     /pseudo
     CDS             628..1277
                     /locus_tag="KPE55_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            1818..3173
                     /locus_tag="KPE55_002880"
     CDS             1818..3173
                     /locus_tag="KPE55_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0493924.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(3291..3959)
                     /locus_tag="KPE55_002881"
     CDS             complement(3291..3959)
                     /locus_tag="KPE55_002881"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG0493925.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(4026..4454)
                     /locus_tag="KPE55_002882"
     CDS             complement(4026..4454)
                     /locus_tag="KPE55_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0493926.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            4561..5154
                     /locus_tag="KPE55_002883"
     CDS             4561..5154
                     /locus_tag="KPE55_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG0493927.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            5273..6208
                     /locus_tag="KPE55_002884"
     CDS             5273..6208
                     /locus_tag="KPE55_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG0493928.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(6480..7424)
                     /locus_tag="KPE55_002885"
     CDS             complement(6480..7424)
                     /locus_tag="KPE55_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0493929.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            7742..9172
                     /locus_tag="KPE55_002886"
     CDS             7742..9172
                     /locus_tag="KPE55_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0493930.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         3390 a         1288 c         1135 g         3364 t
ORIGIN      
        1 taggaattag ttcaaaataa aaaagagtgt atatattgaa attactacat ttcaatatat
       61 acactctttt taaatttctc agttttccat ggaactttac tttatttatt tctgtattag
      121 ttgtattttt tatgcatttt tctttaaaat catattttct gattcaactc ttatattata
      181 aaatttcttt actaaattat tctctttttc tatatcttca tatatatttt ttaatcgcct
      241 tttacctaat cttttatcta acattactaa tgatttaact aatatattat atgattttat
      301 cgcatcttct attgagatat ttaaacatat atttactgct tcataaaaat caaattcatt
      361 aaaaaatcca tttttattta caatcttata tccctttgca tctctttcta aatactcttc
      421 ctaaatactc ttcaaaactt tcatcatcta atctaaatcc tatatcacta tgaaaaatag
      481 gttttgcatt tggatatagt tctattgttt tatcaaaagt ttcgaatacc aagttattat
      541 tattcgattt tccaacagtc cactataaac aataataagt ttgttgctcc aaataatgta
      601 acacttgtag aaagatgtta cagatatata aaagatagag aatgtagttt ttctcctaat
      661 actattagaa atagaaaaaa tatgattaaa aatcaaattg aagttttctt taaagatatg
      721 aaattaatag atataactcc ttctatttta cagtcttata taaataacat atacaatgag
      781 cacatgttaa attcaacaaa aaaccaagtc gattttataa aatcagtatt gaaagaatct
      841 tatagattaa aagaaatatc agaaaatatt tgtgattttg ttacaacccc aatcaaaaaa
      901 aatagttcaa cttctaagct ttatacaaaa caaaaagctc aattattatt agaaaaatca
      961 aaaaatattc ctataggtat accaattttt ttgatgctaa ctcttggatt acgttttggt
     1021 gaagctgtat cacttatatg gtcagatgta gaccttgata aaaaatctca catgtaaatc
     1081 aaacattagt ttatgtatac aataaaataa tcttcaaagg ccctaaaacc cctagattca
     1141 aaagaagatt atttgttcct gatgaactta taagtttgtt taaaggggaa aaattaaaac
     1201 aaaataagtt aaaattacag ggtatattga aaaatgaatt tgatttgata tgcttaaata
     1261 aaagattcaa cccgtgagtt caaccaactt ttgttgttca ttttaaaaaa ctattaagag
     1321 ataataatcc tagagatata aaagtacatg aattaataca tgcaaatgcg actttgatat
     1381 tactttcagg tactaacata aaagctattt ataaaatatt agtacatgca gatataaaaa
     1441 tagctatgaa tatatattcc atattctcac ttattagatg aaatgaataa agaaacatct
     1501 gaaaatataa gcaaaatact attcaaataa tgttcagttt tatgtcaatt aaatgtcact
     1561 caaagtgctt tttttccatt tgtcagttaa atgtcactta aagtaccttt ttcctatttg
     1621 tcagttaaat gtcagtaata tataaataag atatatttat aagaccctta aaatcactat
     1681 tatttaataa aatttataat tatatactat ttgctgttga cattggtatt ataccatagt
     1741 ttatcattta tattagaata aaattattaa ttaatagttt cgtaatttta taatagattt
     1801 acagggagtg ataataaatg gatacaaaaa tatctataaa cgaaaacatg agtctaggta
     1861 aaagattttt taaataccta gcaccatctg tagttgcaat gtgggttttc tcactatata
     1921 caatggttga tggaatattt gtaagtaaag gagttggaga attagcttta gctgctgtca
     1981 atatatcaat gccttttatt aattttatat ttgcagtttc tctattattt tcaactggtg
     2041 catctaccat aattgcaatc tatctgggga aaaaagatat taaatctgct aacgaagtct
     2101 tttcatttaa cttagtatca ataattatat tatcaataat tatattagct ataacttttt
     2161 ttaatttaga tagattagca ctatttttag gagctacaga atctacaatt ggtatggtta
     2221 aagattattt aggaataata atatttttta acggattctt tatagtgtct tattctttag
     2281 aagttataat aaaaactgat ggctttccaa tattagctac tgttggtgta ataatatctg
     2341 ctcttacaaa catcatactt gattacttgt ttgtaattga atttggatgg ggtgtaaaag
     2401 gtgctggtat tgcaactggt ttgtctcaag tcttttctac tattttcttc ttaatacatt
     2461 ttttaagaaa aaactctact ttgaattttt caaaatttag aattgatttt aagacactta
     2521 gaaaaattgt atttataggt tttcctgatt caactactga attaagttgt ggtatagttg
     2581 ttttactttt taacttaagt cttactaaat atattggaga aaatgctctt atttattata
     2641 gtgtcataaa ttatataaat acattagtac ttatgactat gatgggtata actcaaggta
     2701 tgcaaccact tactagcttt tattatggtg caggaaatat agataatgta aaaaaattat
     2761 taaaaatggg gataaaagcc acaataatag catcagttgc tgtatttgca atttgtatgg
     2821 ctttttcagg accaatagtc tctttattta tacatccaga agaaacaatg ctattcaatg
     2881 aaggagttcg tgtatttaaa atattctcta tatctttctt attggtagga attaatgtaa
     2941 taatttctgg attctttgta tcagtagaaa aaccatcaat ttctactgtt atatctcttg
     3001 gtagagggct tgtaatagtt gttttaagtc taatttcaat gatactaata tttggtggac
     3061 aaggaatatg gatgactact attgtttctg agtttatatg tcttatttta tcattagttt
     3121 ttctaaaaaa gaatttttct acattagact ctaatctaaa taaggttgct tagatgataa
     3181 gagtgacaat atatgaaaat atctaaataa aaaataccta ttatacaatc ttgcaaatat
     3241 taaacctttg caaaaattag gattatataa taggtatttt atttatgtaa ttattttatt
     3301 tctaaatatc catagccatc ttttattaaa gttcctttta cttcatattc tacaggtttt
     3361 ttaaatattg gaccatctac gacaaatgta tgatactctc catgttctcc acatgggtct
     3421 gcaccagcat tttttatctt ctccacaagt tctctagtta atttttttcc taaaaattcc
     3481 tctcctaagt gctttaagtt tacttttgtt acaacactac aaaatccact gtctataaat
     3541 tcatatacta aatcctctct actctcttgc cataatggta aatctacttt cataccaact
     3601 gcttcacatc tatcagtatc ccattttcta tgactttcta tatctatatc tccatatgca
     3661 catatcgttg ctcccatgtt tttagctttt attaaagccc tctcaaagct ctcttcatat
     3721 ccttctacgc cacactcagc aactaataaa ggtaatccta tgttgctact tacttgctta
     3781 agcatattat attctaaatt atgtgtccat gactttccat gttcttcact tatagttgtc
     3841 aagagtgcca caggcttata tccttttttc aacattcgat ttaatgctaa tatactatcc
     3901 ttaccaccac taaatgacat tacaaatttt tcatcagcca attttaacac ctctttcatc
     3961 taactttgtt ttattaacat aattatacca aatttttgag ttgcatagca aattatatta
     4021 aattgttaat tcactataaa gaatgtccct aatattaaaa gtataacccc tatgtatttg
     4081 tataaatgca ttgtttcttg tcccaaaatt acgctatcaa taaaaaatcc aactaaaact
     4141 tgggatacaa gagttaatat aagtgtattt gatactccta aatttggaat tgcctttact
     4201 gtaaaatata ttattagacc tccaaatact cctcctaata acaacttata attaaaacca
     4261 tttatagata ctagacactt taagtcacct gtaactatta tacttgccaa aaaaaatatt
     4321 gctcctacag ttaagcttat aaaggtggcc actaatgctg ttgtactttt tcctagctct
     4381 ccatttataa aagcttcaag agctgtagaa ccacctgcta ataatgcaaa tattattgcc
     4441 cataattttg tcatggacaa acctccttct atataattct attaatagaa gtttatgata
     4501 caacctatta attaattact aagtatagtt taatttttat acactaggct ttatttggat
     4561 atgaatttct gttataattg ttctatcgat tttggaggtg attttatgta cacaaatttt
     4621 aatatcgatt ttaataactt taataaaaaa gaaaatgcta ttaaatttat gttaatggga
     4681 gtattactca ttatattagg attattatgc cttacattta agacattagg tataaaatta
     4741 atttcttgga cttttggtat tgcactttta ttctttgcat atttaaattt aaaaaatatt
     4801 aatgagttaa aaagatatgc tactaaagaa gaaattaaac cctctattaa tatccaatgg
     4861 gttctaataa ttgcatgtat attacttttt gtatttcctc aaaaaataca aagcatattt
     4921 tcattatttt taggatttta tttaatcttt aatcaactag tagcacttgt aaatagtaag
     4981 aataatcctt attctaaatt tacaacttgg aatatagtta agattctatt tggtatatgc
     5041 cttatattat ctccactttt tttatctaga tttatagtat cgataatgtc ttttttcata
     5101 atattatttg gactagttct atttttctct ggaaatacag cgagaaaata ttaattattt
     5161 tctcgctgta tttttatatt acttaaaata gaatttgttt aagttatcta tttgtggtaa
     5221 tatgtttttt gtacaagctc agcgtacaat tttattagaa ataggtgatt ttttggataa
     5281 atttaaatat gcttttgata ataaaagata tcatacttgg aattactatc ttagaaatac
     5341 ttttggagaa aaagttttta aggtatctat aaatgcagga tttacttgcc caaacattga
     5401 tggaagtctt ggttatggcg gatgtaccta ttgtagcaaa gaaggctcag gtgattttgc
     5461 tggaaaccct aaagataatc ttatcagtca attttataat ataaaagaaa tgatgttaaa
     5521 aaaatggcct catgcaaagt atattggcta ctttcaagcc tatacaaata cgtatgctcc
     5581 tcttgaagtt ttaaaagaaa aatatgagac tatattagaa cttgaagatg tcataggtct
     5641 ttcaatatcc acaagacctg actgtttgcc agatgatgtt gtagaatact tatctgaact
     5701 aaataaaaga accaatttat gggttgaact tggacttcaa acaattcatg attctacatc
     5761 taaaattata aatagaggtc atgattataa aacatttgtt gaaggtgttg aaaaactaaa
     5821 gtctaaaaat ataaaggttg tcgtacacat tataaatggt cttcctggtg aagattataa
     5881 tatgatgatg gaaacagcaa aggcagttgg taaaatgggt gtagacggta taaaaatcca
     5941 tctattgcat gttataaaag atactcctat ggaaaagatg ttacaaaatg gtatgttaac
     6001 tcttatggaa caagatgaat atatcaatct tgtctgtgac caattagaaa tacttcctga
     6061 aactatgata atccatagac tcactggtga tggtaagagg gatgaactag taggtcctat
     6121 atggagccta aaaaaatggg aaattttaaa tcaaattgat gatactttaa aggctagaaa
     6181 ttcttatcag ggctgtaagt ttgtttaata caaaataata actatagaaa tggttatata
     6241 aatatagtcc tttctatagt tatttagtat ttacaaaaac aaaatattaa tttgtacatt
     6301 tatacaatat ataataaaaa atagatagtc taaactgtat ctacttatat tttatttgta
     6361 ctgtccactt tattctataa aatttataaa tagttatttt ttaataccaa atataatttg
     6421 gtataatttt tgttggactc taaaatgtat accaataaat cctttttaag tatataattc
     6481 taaatacttt cagttttttc aatctgcttt aacttgttgg acttataaaa tattcttatt
     6541 tcatcataag aaagattaag tgctactact gcagatatta acatcaacat cactccaaat
     6601 aacttcccta aggaaaaact ttctcctaat atagtgcaac caattattac tgaaccaact
     6661 aattctactg atgccacaac acctgcaact gacaactcta cacctttaga tatacctgct
     6721 gcataaaaaa tatatgcaag tgcagctgga acaatgccta acattagcat acaagataaa
     6781 acatctaaat ccttagcata atttaagatt tcccaaggtc ttgaagaagg aatcataaat
     6841 atagctccaa ataagaaact gtatactaat attgttgaac ttgaaaattc ctttaaagca
     6901 tttttactaa tagttggcat aagtgcatat gctattgctg ataatacccc aagtaaaagt
     6961 ccaaaagcgt ttaaaccttg aaaatccaaa cgtccaccag tcactgccat aattgcacca
     7021 ataaaacaaa gaataagtga aaataatttg cttctagtaa tattttcttt ataacatatt
     7081 tttgaaaata ttgctaaaaa cagtggtgat gtatatagta aaactgctgc aattgataca
     7141 cccgcaatgt caattgcttt aaaataacat aaattaaaca ttgcctgaca tataatacct
     7201 attattactg aatatataat tccttttttg cttatcttta agatttgtgg tgtccttata
     7261 gatgaatata cgcctaaaac tataaatcca aaaaacaatc ttgtaaatgc tacttgttct
     7321 gatgtcagtc catttcccat taaaaacttt ccaaataatc caagtgtagc ccataataac
     7381 cctgctattg ctataaatac atacccttta cttttttctc ccattttaaa ttcccttcta
     7441 tttttattta gtcaatttac actcttcttt ttaagcaaaa actatgccat cttaaatcaa
     7501 tatatatcga ctctaaaacc ctagtctttc caataatact aataaatttt caattttttt
     7561 atatttattt ttcacttact ttaagttgat ttagacttat tttaattgta aaatgagtta
     7621 tatataactt atttaaagtt aaaatagact gaaacttata tataaatatc aaattttaag
     7681 tatattaatc aattaaacct attttaatat atcattaaaa atttaggagg aaaatataaa
     7741 aatgaattta gatttggaat tttatcaaaa aatattagaa gcttcccatg atgaaatatg
     7801 tgtttctgat gataaaggaa taataatata ttgtaataaa gcatttgaag aaaattatgg
     7861 tctaaaaaaa gaagatattt taggaaaaaa tgtttctttt ttagaagact ctggatattc
     7921 aacaaaaagc cccataccaa ttgtattaaa aacaaagact aaattttcac tggaacaaga
     7981 tactcaaaca gggaaaaagc ttatcataac tgctacccct atatttgatg aaaatggaaa
     8041 cttagaattt actgttgaaa attgtagaga tataactgaa cttaataata taaaaaataa
     8101 acttgaagac acaaaaaagc aagtaaaaaa atataaatct gaagtagaaa cactatacag
     8161 aactgcactt agaattgagg atactgtaat tatggacgga atagttatga gaccaatcat
     8221 aaacacagta aatcatgttt ctaaaacgga tgtaagtgta cttttattgg gtgaatctgg
     8281 aacaggaaaa agctcacttg ctagatatat acaccacaac agcaatagat ctaatggccc
     8341 atttataaca ataaattgtg caactatatc tccacaactt cttgaatctg aactttttgg
     8401 atatacttca ggtgctttta caggtgcaag tactaaaggt aaagttgggc tcgtagaatt
     8461 agctaatgga ggaactcttt ttttagatga aataggagat ataccacaaa atctacaggc
     8521 aaaatttttg caattaatac aggatagaac ttttactcca gttgggagct taaaaaataa
     8581 aaaggtcgat ataagaatta tatctgctac aaatgttgat ttagtttcaa aagtaaaaga
     8641 aaaaaagttt agagaagatt tatactatag attaaatgtt atagaaataa aattgcctcc
     8701 tcttagagaa agaagggata atctagtgga aattataaaa tattatttta ataggtactc
     8761 aagtgacttc aatctaaata aaactatttc taaagaagct atggatgcta ttgcaaatta
     8821 tagattccca ggcaatataa gagaattaca aaatataatt caaaaaatct tgcttacttg
     8881 tacagataat catataacaa tagatgactt accaaacatt cttacaaaaa atatacatat
     8941 taccaataat ggaaataaaa cacatatatc ccaaattaat aaggttatta tatctgactc
     9001 aaaatctact aattataaaa ataaaaattt tgacacttta attaaagagt atgaaaaaaa
     9061 tataattttg gatgcttatg aaaaatttgg tagttcttat aaagtagcca aacaccttga
     9121 aatctcacag tctaaagcca ataggttaat tagaaagtat actaatactt aatttta
//
LOCUS       DAELNY010000149         9165 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.151, whole genome shotgun sequence.
ACCESSION   DAELNY010000149 DAELNY010000000
VERSION     DAELNY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.151"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(26..679)
                     /locus_tag="KPE55_002887"
     CDS             complement(26..679)
                     /locus_tag="KPE55_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0493931.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(924..2789)
                     /locus_tag="KPE55_002888"
     CDS             complement(924..2789)
                     /locus_tag="KPE55_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG0493932.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2794..5214)
                     /locus_tag="KPE55_002889"
     CDS             complement(2794..5214)
                     /locus_tag="KPE55_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG0493933.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5228..6670)
                     /gene="glgA"
                     /locus_tag="KPE55_002890"
     CDS             complement(5228..6670)
                     /gene="glgA"
                     /locus_tag="KPE55_002890"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG0493934.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6699..7829)
                     /gene="glgD"
                     /locus_tag="KPE55_002891"
     CDS             complement(6699..7829)
                     /gene="glgD"
                     /locus_tag="KPE55_002891"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG0493935.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7831..8991)
                     /locus_tag="KPE55_002892"
     CDS             complement(7831..8991)
                     /locus_tag="KPE55_002892"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG0493936.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2882 a         1698 c          899 g         3686 t
ORIGIN      
        1 attataaaaa aaataaagag aaactttata aaagcttctc ttttttcatc tctgctatag
       61 gtatcgtttt ccaatgttct ctcaaaactc tttcaacatt ttctaaatca ttctccctca
      121 tagattttac tacttcaata tgttgattat tgctgtatgt caacatttta tataactcat
      181 cttcattatc actagtatat gactttgaca taaaattata tcttaaagag ttcaatagat
      241 ttactaacac ctgattatct gatttagtta tgtatatatc gtgaaattca ttacttaatt
      301 ttaaatagct attgtaattt ttatacttta tagagaccgc catcatttcc gttaattctt
      361 ccatttttac taggtctgat tcagttaaat tttgtaatgc tgaagaaccg gctaacgcat
      421 ccaatactcc aattacggaa taggtgtcta atttctcctt ttcctgaaat tcttttacta
      481 caaatccctt attaggtatt ttttctagta aattttcacc tgctaaccta gttaatgctt
      541 ctctcgctgg tgttctacta actccgatag atttacaaat ctcagattca gtaattctct
      601 gattagcttc atactctcca ttttggattt tctttgatat atagtcatat acttgagttg
      661 taagtggatc taccttcata agacgttcct ccatattaag attataaaag ttaaatctcc
      721 ctacaacttt ggtagagagt tcagttttat cttactaatt tatataatat cacaattatg
      781 atatttttta tacttctttg taatattttg aaatactaaa attcttatga taatttatac
      841 aaaaaaagta gaaaaagttt tgataaaaca aaactctttc cacattcacc ttattttcta
      901 tttatttttc taaaaaaaga tatttaattt actctaaaaa ttctaaatga tttagagtct
      961 aaatctactt tcaactcatt attttcaatt tgtatagtac tatttgcatt tttaagacta
     1021 tactttatag gtatttcctc aataaaacta ctctctatac ataatgatat actttccaca
     1081 ttttcacttc tatttactaa aattaacatt ctatcattat tttcattaac tctaataaaa
     1141 gaaaatacag aatcattatt agtatatata aattctgtat ttccagaact taaaagttta
     1201 ttcttatttc taatttttat aacatctcta taaaaattaa gcatatcttc gtcttcattt
     1261 ttccatggat aagttcttct attatctggg tcaacacctc cacaaagacc tgcttcatct
     1321 ccataatata tatatggtac tccttcaaat gtcatctgta tagctacagc aagcttcact
     1381 aaatctacat catcatttaa ttccgtttta gctcttgtaa catcatgtgt tccaattaaa
     1441 tttaaattag acctaaatga ttcttttgga taattttctt taatctgcat atatctatta
     1501 caaagttcat atgaattaat ttctcctttt aagaaagaaa acatgttatt tctaaaagga
     1561 taacccataa cagaatctaa ttcttcacct aataaataac ttcttctctg accatagcta
     1621 atcttatttg atgcatcttc ccatacttca cctataagaa ttgagtcatc atctgccttt
     1681 tttaattcct tttttaactc tcttataaac ttagttggta attcgtctgc aacatctagt
     1741 ctccagccct taatacccat atttacccat ttatttataa cgctatcatt gtcatatatt
     1801 atataatcca tatagctatg ttctaattca tttatatttg gcaatgtctt aacatcccac
     1861 caacttttat atttttgagg aaattcttca aacatatacc atgaataata tggagattct
     1921 ttagattgat atgcccctaa actattatag tttccataca tattaaaata tttactatct
     1981 gctccagtat gactaaaaac tccatctaat actattgata tacctttttc ttttgcttta
     2041 tcaataagtt ctttaaatgt atcttcatct ccaaacattg ggtcaatttt tttatagtca
     2101 cctgtatcat acttatgatt actagaggct tcaaatatag ggcttagata tagtatactt
     2161 acacctaatt tttttaaata tccaagttta ttgattatcc ctcttaaatt tccaccatgg
     2221 aaatcccatc ttataacatc cccttgacta tcctttatgt acataggtgt atcttcccaa
     2281 tttccatata tgaaactatt ttttttagga ttatcaacct taccactacg atttccatta
     2341 ttgaatctat ctacaaatat atgataaagt attccttctt tgtaccatgt tggtacttta
     2401 aaatctttgt aaacagtgat ttgatattta ttaatatcac tataattata ttcacaagaa
     2461 tatccattat ctctgttttt tccataaaat aaagtcttta tctgaccatc catttcaaca
     2521 tcaactttaa agtaataaaa ataaacacct gtaccatcaa acttttcaat cttaccacaa
     2581 aagcactttc caagtttatc attatctaat tcttcattta aagttattgt tctcatttct
     2641 tcttctctat ttatttctag ggaaatagac ttgatattgt atccctcatt tactttaaca
     2701 caaatggtca attcctcatc aaattttaaa gctccaaacg gagccttaaa atttttatcc
     2761 caagagttat attctattac actttgcatt taatcacacc cttttaaacc aaatgtcttt
     2821 tgcatactca gaaattgttc tatcagaaga aaaaaatcca gagttagcta tattaactaa
     2881 agacatttta ttccattttt ctttatctct atataacctg tttatatctg cttgagcttg
     2941 accataattt tcaaaatctc tcaatacaaa atactcatca ttgtaagtag tgagtgaatt
     3001 ataaatactt cttccttctt cgccaagatt aggaataaat ccatttatta aatcatctac
     3061 tacccttttt atatccatat tagaatggta taggtcaagt gaagaatatc caccatattt
     3121 attataattt agtacttgtt ctgcactaag tccaaataaa aacatatttt ctttaccaac
     3181 ctgttcacat atctctacat ttgcaccgtc aagtgtagcc aaagttatag caccattcat
     3241 cataaacttc atattacttg ttccagatgc ttccttagtt gtagttgaaa tttgctcact
     3301 aacatttgct gctggtatta ttatttctgc taatgatact ccataattct ctaaaaatac
     3361 tacttttaac ttgtccttta ctctaacatc attatttata gtacttgcaa cagaatttat
     3421 aaatttaatt atacatttag ccaaataata tcctggtgct gcttttgcgc caaatataaa
     3481 agttcttggg tcaagattca aattaggatt atcaagtaat tcatgataca tatgaagtac
     3541 attaaatata ttcagtaact gtcttttata cgcatgcaat ctcttaactt gaacatcaaa
     3601 tatcgaactt gggtctacat ttaaatcgta tttatctttt ataaactttg ctagatttgc
     3661 tttattattg tattttatat catcaagtct ttgtaaaaca gaggaatcat ttttaaattt
     3721 ttcaatattt tttaattgta atgtatcttt cttccatgaa tcaccaatta aatctgtaat
     3781 taaattgcta agttgaggat ttgaacttat aagccatcgc ctatgtgcaa ttccattagt
     3841 cttattatta aatttatttg gttcatcttg atagaaatct tttaatacct ctgtttcaag
     3901 aatatgagta tgaagcttag caactccatt tacactatga cttgtgacaa tacataagtt
     3961 tgccatattg atattgtcac agtctattat actcattctt tttattctat cttggtcata
     4021 tcctttatta tttaattcct ctacatatct tctatttatt tcttctatta tcatataaat
     4081 tcttggtaat agttctttca tcatacttac aggccatttt tccattgctt ctgacattat
     4141 agtatgatta gtataggaca ctgttttttt agttatttcc catgcttcat cccaagataa
     4201 attttcttca tcaagcaaaa ttctcataag ttctggtatg cataaggtag gatgtgtatc
     4261 atttatatga attgctacct tatcgaataa attattaata tttaatttgt tctttttata
     4321 ttttcttatt atatcttgta tccctgcact tacaaaaaag tattcttgtt tcaacctcaa
     4381 caacttacct gcatagtttg aatcatcagg atataatacc tgtgatattt cctcagtata
     4441 atacttatgt tttaaagcct cttcatagct accagaataa tttagagcat ttgaggtaag
     4501 ttcaccaaaa tctctctttg gtatttcact cttaaacaat cttaaagtat ttatacattg
     4561 gttttggtat ccgattattg gtatatcata aggcattgcc attacaggaa ggtagttttt
     4621 atgaattact tttaaatgac taccttcctt gattaattct acctcaccac caaacttcac
     4681 cattgttgct tcatttggtc ttacagtttc ccatgcatat cgtccttctg taagccagtt
     4741 atcaggcact tctacctgat aaccatttac aaatttttgt tcaaacagac catatttata
     4801 ccttattcca tatccttgac cacttatgtt caaagatgcc atagaatcta aaaagcaagc
     4861 tgctaatcta ccaagtccac catttcctaa ggctgggtct acttcagctt caattaaatc
     4921 atccaagttg attcctaatt cagataaacc ttctctaact tcttcttcaa tatttaaatt
     4981 taataaatta gattttaact gtctccctat taaaaattct aaactaaaat aataaacttc
     5041 ttttttctta tctaactttg tagcaaccca tttttttgct atctcatctt tcaatagact
     5101 acataataca tttaacaatt gttcatcagt agcttccttg atagattgtg catataagct
     5161 atacattttt acttcaaatt tttgcttgaa tttctttttg cttattgtta ccaaaaaaac
     5221 cactccttta atcacatatg tctctatatg tttctatata ctccttagct gattttttcc
     5281 aactattatc tgttttcatt gcattttcaa ctaatttaat ccatttttct ttgtccttaa
     5341 atactttgat tgcatttttc aaacagaaaa acatctcatg agcattgtaa tttttaaaac
     5401 taaatccatt gccttctcca gtaaatttat tataaggtgt tactgtatct ctaagccctc
     5461 cagtctctct aactattggt aaagaaccat atctcatagc taacatttga cctataccac
     5521 aaggctcaaa taatgatggc attaagaaca tatcacttgc agcatatatc tgttgagcca
     5581 atgatgcatt aaaagtaatt ctagctgata ctttatctga aaaattagaa tcgtaatagt
     5641 tgaacattga ttgatattga tgttctccag ttcctaaaac gacaatctgt aaatcttctc
     5701 tcataagttc tggcatcatg tatgctatta aatcaaatcc tttttgagaa actaatctcg
     5761 aaactatacc tatcaacgga atagatgaat cttttttaag tcccaatata tcttgtaatc
     5821 tcaatttatt ttctactttt ttgtcaattg agtctacatc ataatgaaca aatatatctt
     5881 tatcttttgc agggtcattt aaatcataat ctataccatt taatatacct tttaattttc
     5941 ctgactcttt tctaagtaat ccatctaatt gttcaccata aaaagatgtt tgtatttcat
     6001 ttgcataagt aggactaaca gttataattt tatctgcaaa atttatacca gctttcataa
     6061 aatttattga gccataatac tctatatctc cattatcaaa gtatttttgt ggtaaatcca
     6121 atatatcata taaaacatgc ttatcaaata caccttgata ttggagatta tgtatagtat
     6181 acatagtttt tatattttta tacccctcaa gggttgcata tctttctttt agaattaatg
     6241 gtaacatccc tgtatgccaa tcatttatat gtattacatc tggataaaaa tcaattctac
     6301 ttatagcttc taaaactgca tctgtaaaga aagtatatct ttctgcatca tctccatatc
     6361 catagatact ctttctttca tcttctcttc taaaataaaa ttcattatct ataaagtaaa
     6421 attttacacc atccaattcc atttctaaaa gaccacagta ttgatttctc catccaacac
     6481 gtacactaaa cacagctatt tcttttagtt gatcttttaa ataacttggt atagttgaat
     6541 atttgggcat tattaccctt acatctacac cttctttttt taattcttta ggcaatgcat
     6601 aagaaacatc tcctaatcct ccagtttttg caaagggcca acactctgct gttacataaa
     6661 aaaccttcat tagtcattcc cccttctaaa aaatttattt atatatttgt attcttttcc
     6721 actacaagag gatatttttc atctcctctt aattctttcc catttgatac actcgtattt
     6781 ttatctaata ttgcattata tagttttgca tttgacttta ttacacaatt ttgcattact
     6841 attgagttct ctataactgt tcctttttca acatttacct ttctaaatat gatactatct
     6901 tttacagtac cttctattaa acaaccagat gctataaagc tattttctac tttagcacta
     6961 tctgtataaa ttgtaggtga ttcattcttt tcttttgtaa atatctttct ttcagaatat
     7021 aataattcat ctgcaacttc taaatttaat aaatctttgc tcatttcaaa atatgattca
     7081 acagaattta cacattttag atatcctgta tattcatagg ctcctatttt catgttttct
     7141 gcttcttcaa taataaattc ttctaaataa caataattgc ctttatttac aatgttatat
     7201 atacattcaa caaaatcttc acgcttcata atatatgtct ccatacatat gtttgctcta
     7261 gggaacttac ctaaattatt tcctacattt attaccctat tgttactatc taaatttaga
     7321 gttaagttac cagcaaaatc tttatttgca ttatttacat ttttatatat tatagttata
     7381 tcattatgag attttttatg gtattccaat gcttgagaat aatttaaatt gcatagcata
     7441 tagcttggag ctattaaaat atattcttct tcactctttt ctatataatc tagattagct
     7501 aagatagtat atatatcacc ttctctatgt gagtaagtat ttctatactt agtgttttct
     7561 ggactaaaaa ttgataggcc accttttttt ctacttaaat cccaatcttt accactacct
     7621 atatggtctg ttaatgatct gtatttttct tttgcgaata ttccaacttt agtgattcct
     7681 gaatttatca tattagataa agcaaagtct attattctat atcttccagc tattggtgta
     7741 gatgctattg gtcttccata gttaagtttg tttattgctg ggtctccttt tttgtttaaa
     7801 tttattatcc ctaaacattc atttctcatc ttaaagacct ccttctatta atacgcatct
     7861 aggttcgata ttttcatact cagaaactac atttatttca ttatttacat tctttatctg
     7921 aacattatca tttatagttg cacactcacc tatcatagct ttttctatag ttacattttc
     7981 acctattaca acatttggca ttattactga atcaattact ttacttcctt taccaacaac
     8041 aactccatgt gataatacag agttacctac ttcacctaaa attctacaac catcagctat
     8101 cattgatcta tgaacatttg catttttacc tatatattgt ggaggcattg ccatagtatt
     8161 tgtatatatc ttccacttag ggtctgctaa atcaagtgtt tctggagatt ttattatatc
     8221 catattagca tcccatagac tttgtatagt tcctacatct ctccagtatc ctttaaatgg
     8281 ataagcatac attccaacat tatcttctaa cattttaggt attaaatttt ttccaaaatc
     8341 atcataattt atttctggat ttttttctgc ttccttaaaa taatttctca acattttcca
     8401 atcaaatatg tatactccca tagatgccaa attgctctta ggttcacttg gtttttcttc
     8461 aaattcatag atacttgaat cttcatttgt attcattatc ccaaatcttg aagcttcatc
     8521 ccaaggaact tcaatcactg ctattgtagc tttagagcct ttttccttat gaaattttaa
     8581 cattttgcta taatccatct tgtatatatg gtcaccagat aatatcaata catattctgg
     8641 attataggta tctacaaagt ccatattttg atatatcgca tgtgctgtac cattatacca
     8701 atttccttct ttttcattca taaatggttg taaaacatag acaccaccat ttattctgtc
     8761 caaatcccaa tgacttccca tgcctatatg tgaatttaat atcaacggtc tatattgagt
     8821 taaaacacct acagtatcaa taccagagtt tgaacaattg cttaacacaa aatctataat
     8881 tctatactta cctccaaatg atacagctgg tttggcaatt ctttttgtaa aaacacctaa
     8941 tcttgaacct tgccctcctg ccaaaatcat agctaacatc tcttttttca tttaatgaca
     9001 ctcccctcat ctagtaatta atatttataa ttgcattttt acttttataa actttatcag
     9061 tcaataaatg tcgtgtataa atgttgctta aataatacct taataaaaat aaaataatat
     9121 caactataca attgatataa tgtatacccc ataatggact taccg
//
LOCUS       DAELNY010000150         9148 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.152, whole genome shotgun sequence.
ACCESSION   DAELNY010000150 DAELNY010000000
VERSION     DAELNY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.152"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            52..252
                     /locus_tag="KPE55_002893"
     CDS             52..252
                     /locus_tag="KPE55_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG0493937.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            306..1469
                     /locus_tag="KPE55_002894"
     CDS             306..1469
                     /locus_tag="KPE55_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0493938.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(1680..2102)
                     /locus_tag="KPE55_002895"
     CDS             complement(1680..2102)
                     /locus_tag="KPE55_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG0493939.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(2123..2959)
                     /locus_tag="KPE55_002896"
     CDS             complement(2123..2959)
                     /locus_tag="KPE55_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0493940.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3257..3853
                     /locus_tag="KPE55_002897"
     CDS             3257..3853
                     /locus_tag="KPE55_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG0493941.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILSPIYTVLLKIVYLFV"
     gene            3883..4602
                     /locus_tag="KPE55_002898"
     CDS             3883..4602
                     /locus_tag="KPE55_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG0493942.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            4604..5143
                     /gene="scpB"
                     /locus_tag="KPE55_002899"
     CDS             4604..5143
                     /gene="scpB"
                     /locus_tag="KPE55_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG0493943.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            5305..5934
                     /locus_tag="KPE55_002900"
     CDS             5305..5934
                     /locus_tag="KPE55_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG0493944.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            5888..6316
                     /gene="ytfJ"
                     /locus_tag="KPE55_002901"
     CDS             5888..6316
                     /gene="ytfJ"
                     /locus_tag="KPE55_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG0493945.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            6564..7004
                     /locus_tag="KPE55_002902"
     CDS             6564..7004
                     /locus_tag="KPE55_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0493946.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            7020..8471
                     /locus_tag="KPE55_002903"
     CDS             7020..8471
                     /locus_tag="KPE55_002903"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG0493947.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCFVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(8562..>9148)
                     /locus_tag="KPE55_002904"
     CDS             complement(8562..>9148)
                     /locus_tag="KPE55_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG0493948.1"
                     /translation="TKNILPYSYTVEKIICTLLVIIMALEQLLLISSGNYSTLNSLPI
                     GINYICIYLCIAILIFKQYHLFNIFFSWSLVCSVGELIFSKNLGYEFPSLIYFIFIFS
                     KCLIIYADIYMVDVRKFRVNRYALRDNLAICFIYFSFIFLLNTFTNSRYYYGFLSHST
                     TAIFTFIFVTSIMYIPALLFNRDTFILEKKKKSK"
BASE COUNT         3648 a          941 c         1504 g         3055 t
ORIGIN      
        1 tttattagta aaaactactt atatataaat tttactgata ggaggattct tatggatgat
       61 gtttcaagac aaaatgctat aaaagcttta aaacaaacaa aaatggaaat agcaggagag
      121 tatggaatga attatgaaga tgcttttgaa ataatagaaa atgcgtctaa taaaggtgtt
      181 ttagaaggtt actttaaaaa gcttgaaaag aaaaaaaatt taggtcaagg tatatctagg
      241 catttagaat aaaaaaagtt aaatgtgaga aaataagttt aaataaactt taagaggtga
      301 catcgatgaa aaaattagct agtactgctt tggctatatt aattgcgctt actcctttaa
      361 gcttttcttt tgcaaataat aaggaaaatg ctgatgccaa ccaactaaat atatcttcaa
      421 aatcagcaat tttgatggat gttggtagtg gacaaatttt atatgaaaag gatgctcata
      481 aaaagcttcc tcctgctagt gtaactaagg taatgactat gctacttata gtagaagctt
      541 tggattcagg caagattaag cttgatgatg aagttcaagt aagtgaaact gcatctagta
      601 tgggtggtag tcaaatattc ctagagccag gagaaactca aaaggttgat acattattaa
      661 aaggtatagc tgttgcttct gcaaatgatg cttgtgttgc tatggctgaa cacttagctg
      721 gaagtgttga aggatttgtt gatagaatga atgctaaagc aaaagagtta aatatgaatg
      781 atactcattt tgcaaataca aatggacttc cagttgcaaa ccactataca tctgctcatg
      841 atatagcttt aatgtctaga gaactattaa aacacgaaat gataagtaaa tacttaacta
      901 catggatgga taaggttgta gtagggaaga aacaagttac agtaggtctt gcaaatacta
      961 ataaacttat aaagcattat caaggagcaa ctggtgttaa aactggtttt actcaagaag
     1021 caaaatactg tttatcagca tctgcaaaga gaggtaatac tcatctagtt gctgcaactt
     1081 taggagcaga gacttctcca gaaagattta atgatgcctc tagcctttta aactatggat
     1141 ttgctaatta tgaaagtgtt aagttatgta gtaaaggtga taatatagca acacttacat
     1201 tggataaggc tgatgaaaac aaagttaaat tggttgctaa agaagattta aatgctctta
     1261 ttaaaaaagg aagttctaaa gaatttgaga agaaaattga aatagtaaaa aatcctaaga
     1321 tgccaattaa aaagggaact gttttaggaa aaataaagat atgtaaagat aaaaaagtaa
     1381 taggcgaagt ggaattgata aatactaagg atataaataa ggcaagctat ctacaaatgc
     1441 ttcaaagaat aattgataat atgatataaa taattatcag atgaaatata attataatac
     1501 acaaagatat gattataata cacataaaaa atacccttat gtaattattt gccatagggg
     1561 tatttttatt taccatatag aagtatttta taatcttatg atttttatag aaatatagaa
     1621 aagtttatgt atcttaaaat atcatttgat ttaatatcat atatagggtt gtttaacaat
     1681 tatctaagta aaggcatact accagtaagt ttattcactt tctttttatt cccataaatt
     1741 gctatcccaa aatactgtaa ctcatcttct ggaacactag caatcttttg tgtaaattct
     1801 tcataaagat tgcaactttg tgctacatct gaaaaatcta caacagttaa atcgttaaat
     1861 tccaaagtat ataatttttt acgtaaatct tttattattt ctttgtttcc ttttaaaata
     1921 ggtacaggaa tgtcaataat tcctaagtgg ctattacctg ttttgtcaat tacatctggg
     1981 ccaacagttt ctggggcatg ttttcctagt gtaattccca ttatagctgc tgtattggat
     2041 ataattccca ttggtaaatt ttcgtctatt accatgacac attttgaatt tttagtttcc
     2101 atttttattt ctccttttta ccttatttaa agttgtttga atcatcattt ataaatatat
     2161 tttgatactg ctttggtgta agaccaataa aattcttaaa aaagtttgta aagtgacttt
     2221 ggtctacaaa tcctgtttgt atcgcagcat ctataggttc tacgcctttt tctaaaagct
     2281 ttttagcttt gtttacccgt atattttcta ggtatctata tggtgtaata ccttttaatt
     2341 tcgtaaaatt acgaagtaga gagtacttat tcatacctgc tatagtgctt aattcgtcaa
     2401 gtactatatg ttcagcataa ttattttcta gataatcaca tactgcttga atttctatat
     2461 ttgtattttc caagttagat tgcaaatttg gctctgtatg ttcttcaatt aattgttcaa
     2521 ttaggaagaa aaaaagctct tcctttttaa aatccaattc ttcttccatg attatatagt
     2581 gtaattcttg tagtagaggt actagctcac tgcgaaatat aacaggttga ttaaattttg
     2641 gtaaataatt tttacctgta atttcaaaga cggttttttt cattatttct ggcttaatat
     2701 taatacatct gtaatcaagt actttatcat ctatttgttc acaagtatga ctatcaaaag
     2761 ggttaaaaag tagcaaatct ccagtacttg tggtatactc tttattttta caagataaat
     2821 atctttgacc tttttctata aaaccaatta cataatgttc atgaaaatgg tttgggaatt
     2881 tttgcattat gcctttaaat ctatatgctt caatttttaa ttccctatca aagcatactg
     2941 tgcgttcttc ttgtctcact taggtaacct cctttcatgt tatacaaagt gtaacacata
     3001 tttttttata cttcttgtat aatattgact aagagacaat aatgtcaatt catgtcaata
     3061 taatattata cttacagtag gtgtaattat tatattttca caatgaatat gatataataa
     3121 taaaagttat aaaataaaaa gcaaatttta attttatgcc tagaagttaa aattattata
     3181 atgtaaaata attttgatta taagctattt aattgttata ataatattaa aaatttaatt
     3241 taggagatgt acattaatgt taagtatgaa tataggagaa atagtagcaa gtttagttgg
     3301 tatagctatg gcaatatcaa tacatgagtt tggccatgct tattcagctc acttattggg
     3361 ggatgataca gctaaagctt atggtagaat gacattaaat cctgtaagac atgttgaccc
     3421 aataggtctt ttggctatgt ttatccttaa gatagggtgg gcaaagccag taccagtaaa
     3481 tccaaataac tttaaaaact ataagatagg aaacctgata gtttcacttg caggcgtttt
     3541 ttgtaatgtt ttgactgcta tactgtgtgt aattatcaat aagtatgtgc atatgtatgc
     3601 tataaataca atagctggat atgtgctttt atatagtatg ggatttgcag cttttaactt
     3661 acttccgata cctccacttg atggatgggg agttatatct acatttgtac catataaatg
     3721 gaatgaatat ttgtataaat ttgaaagtat gagttatata attttattaa ttgctttatt
     3781 tactgggatg tatagaataa tactatcacc aatatataca gtgttgctaa aaatagtata
     3841 tttatttgtt taatataata aacagattaa agagggattg atatgaagta taatatacaa
     3901 ttacaagtct atgagggacc attagattta ctttatgatt taataacaaa gcataaaata
     3961 gatataaagg atatatccat aatagatata actaagcagt atctaaatta tttgaaaatg
     4021 ctagataaaa tggatttaga gataactagt gaatttataa ctatggcatc aaaactttta
     4081 gagataaagt ctaaatatct gctttataag caaaaagatg aagaagaaga cccaagaata
     4141 gaacttatgg aaaagttaga agaatataga aagtttaagg tagcatcaca agatataaaa
     4201 gaaaatataa cttatgttaa tgagagattt tatagaaata aagaagagat aattattgat
     4261 gacaatgtgg atttagaaga tatatcaatt gaagcaataa aaaatatact tccatacata
     4321 tttaaagtta aaacatcaca gatagaaaat gctaatgatg agaaactaga taaaatagta
     4381 aggaagaaaa taatatctgt agaagaaaaa atattatata taagagatat tataaaagat
     4441 aagattgaag ttacttttac aaatattata aaatcatatg aaaatgatga aataatagct
     4501 acttttttat ctatattaga gcttataaaa gaaaaagaaa tagtcgtagt acaagatata
     4561 ttttttgatg atattctaat aagaaaaagt tcggagtgtt aaaatgaaaa gagaagatat
     4621 aaagtatatt atagaatctg ttatgtttgc atatggggaa ccaataagta taaaagaatt
     4681 aaactatata ataaataaag aactttcatc aaaagaaata gagattatgt taaatttact
     4741 tattgaggag tacagagaac aaaatagagg tattcaaata atcaagttag agaataagta
     4801 ccaaatgtgt acaaataaag attatgcaga atatataaag aagattattg aaccaaagaa
     4861 aaagaaaagt ttgagccaag ctacattaga aactcttact ataattgcct acaaacaacc
     4921 aataacaaag gtagaaatag aggatataag aggagtaaaa tgtgataagg tattacaaac
     4981 tttgtttgaa aatgaactta taagagaagc tggtagattg aataagatag ggaaaccaat
     5041 aatttataaa acaacagatg aatttttaaa gttgttgaat atagaaagtc ttgaagaatt
     5101 gccacctata gaaaattatc aagaagtggc aacaaatgaa taaaaatata aaatcacaat
     5161 aatttagtat gtaattttaa attttaaaag atttaaaatg ctctaagttg ttgtgttttt
     5221 ttgtataaat ataaaattat aattctctta aaataaatat ggataattaa gtataaatag
     5281 taattttttg atgataatat aaacatggag cttaatgaat tatttaattt taagtattta
     5341 ataatagctg taatttttat tataagtgta actatgttga ttttaatatc tcatataaac
     5401 atcttaataa tagctgatat aaaaaacaaa gatatttgtt taaagcttaa tataaaatac
     5461 atgtttaatt taataaatat aaatagacaa ctttatccag ctgaaaatag taaaaataat
     5521 gataaaaaag aggggatgaa gaataatata gattcaagta tattattagc tgatgacctt
     5581 ttgagcattt atagattatt aaaaaagata aaaatacatg aactttattc aaatattaat
     5641 tttggaactg gaaatattgg cttaacatct tctgtatatg tattgataaa tactttgtat
     5701 ggaaatttat ttaatatgat tgatgcagaa aaaatgtatt taaatgtaaa tccagatttt
     5761 acaaaagatt atgttttagg aaatataagg atacatataa ggccaagaat aaaagcttta
     5821 tttaatatta taataatgat taataaaatt atgaataaaa ataaaggaaa taaggaaggt
     5881 gatagtaatg agagcaacag gttcgataca gagtcttatg gaaacaactc ttgaaacaat
     5941 taaaggttct atagatgcaa atactattat aggagaccct ataaaaacag atactacagt
     6001 ggttgtacct atatcaaagg ttacaattgg ctttggtata ggaggaggag aatactcaaa
     6061 aggatatgat gataaagata gggaaattga attaaaaaat gaaaaatcag atacgaattt
     6121 tgctggaggt agcgcagggg ctatatctgt acaaccagta gcatttgtag tagttgaaag
     6181 tggagagact agaataatga gtttagatag taatatcaat ctagtggata atatattatc
     6241 tataactcct agagtattgg agaaaattca aaatatatca caaagtaata agaatcaaga
     6301 taaaaacaat atgtaagaat aaaatatttt taaatatgta tatagtttta agtaaagtag
     6361 tggggttaat ataaatccca ctattttgtt gtgtacttta aacatttata tctaataaga
     6421 ttttagttta aaacaattta aatagataat aaagttgata tatagtataa ttataaaaag
     6481 aaaagaagta ttatattata gatatataaa cgcatttgag atataagagg gagttataaa
     6541 tcattagttt tgaggtgaat gttatgagcg ataaagaatt atctattttg ttgttagaaa
     6601 gtatgaataa ttttcataat ttaataaaga ttattaataa cgaaagatat aaaaaagata
     6661 aggttcttac agaaagacag ttctttgcct tagtaaaaat aagaaaacat gataaaatag
     6721 agcttaaaaa tctaagtaga gatttgcatg tatctacttc tagtctgtgt atactactta
     6781 ataaattggt tgagcaagag tatgtatata gggaagaaga tagtagagat agacgaaata
     6841 ctttttatgg aataactaaa aatggagaaa agatattgga taatgaaata ctaaagtttg
     6901 tatctataat aagtgataaa atggattgcc tagatataga taataaagat aaattattta
     6961 cttctttgga agagtctaaa aatataatag aacaattatt ttaaaaagga gaatttatta
     7021 tgaatataaa gtattctaaa gagatattaa aaagtttaga aaatgctata acagtagtag
     7081 ctctatttga gggagaagat gtttttacga catttgataa aataaatatg ataagcagaa
     7141 attttaattc taaagaaaat agtatagata ctgtaacatt aattgaaaat aacaatttgg
     7201 taaatgtttg ttttgttggt tttggtaaga gagagttatt gaatagagaa aaaattagaa
     7261 tcataggtgg gaatttgtgt aaaaaattaa aaaatatatt gaaaaatgaa gcatataaga
     7321 gtagtgatgt taatgtactg aatcttaatt taaatagtga tgaaataggt tgttttgtag
     7381 aagggatatt acttggaaat tataagtttg ataagtataa gacaaaaagt aaggattttg
     7441 aaagaaaaga agttgatact ataactatat ttactgaaaa agaagttgag tgtcaaatag
     7501 aaaaggctaa aatattagct aattcaacta taatagctag aaatctagtt aatgaaccat
     7561 caaacgtaat atatccaaaa acattagcag tagaaacagt taaacttggt gcagagtttg
     7621 ggtttaatgt tgatgtttat gaagaagaaa aaataaaggc attgggaatg gatgcttttt
     7681 tcgctgtttc aagaggttca attaataaac caagattcat agtaatgaga tactttggag
     7741 ataaagaaag tacagatata ttaggtctag taggtaaagg attgacttat gatactggtg
     7801 gatattcttt aaaatcaaat gcttctatgc tagatatgaa gacagatatg gctggtgctg
     7861 cgagtgttat aggtgctatg tgtgctatat ctcaaagtaa attaaagaaa aatgttatag
     7921 cagtagtggc tgcatgtgag aatgctttat caggaggttc atacaaacct ggagatataa
     7981 ttagttctat ggcaaagaaa actattgaag ttttaaatac ggatgcagaa ggtagactta
     8041 cattagctga tgcaatatac tacattataa acaatgaaaa agtaacaaag gtggttgatg
     8101 tagctacatt aactggtgct gctttaacat tgttaggtaa tgttgcaact cctatagtaa
     8161 caaataatga tgatttttat tgtgaattag aaaaagctgc cactttatca ggggaaaggg
     8221 tatggaagat gccaatttat gatgaattta aagatatgat aaaaggagaa gaagcagacc
     8281 ttaaaaacac tggtggtaaa aatgctggat gtataacagc aggagctttt attggtgaat
     8341 ttgttgggaa tactccttgg atacatatgg atatagcggg gacaagtaca tcatcaaaat
     8401 caattggata caaagcaaag ggggctacag gagaacctgt tagaacattg tactatttgg
     8461 cagaaatcta aaaaacaatt ataaaatata atttataaag ccacgtggat atttttgtaa
     8521 tttaagatta tctatgtggc tttatttttt atttaagtaa attatttact ctttttcttt
     8581 ttttcaagta taaaagtatc tctattaaat aaaagagcag gtatgtacat aatactagta
     8641 acaaaaataa acgtaaaaat agcagttgtg ctatgactta aaaaaccata atagtaccta
     8701 gagttggtaa atgtgtttag taaaaatatg aatgaaaaat aaataaaaca tatagctaag
     8761 ttatctctaa gtgcatatct gttaactcta aattttctaa catcaaccat atatatatct
     8821 gcataaatta tcaagcactt agagaagata aagataaaat atattaagct aggaaattca
     8881 tagcccaagt ttttagaaaa aatgagttct ccaactgaac aaactaaact ccaagaaaaa
     8941 aatatattaa aaagatgata ctgcttaaaa attagtatgg ctatacacag atatatacaa
     9001 atataattga ttccaattgg taaagagttt agagtactat aattacctga agaaataagt
     9061 aataattgct ctagagccat aattattaca agtaatgtac aaattatctt ctcaactgta
     9121 tagctataag gtaaaatatt tttggtta
//
LOCUS       DAELNY010000151         9124 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.153, whole genome shotgun sequence.
ACCESSION   DAELNY010000151 DAELNY010000000
VERSION     DAELNY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.153"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..8089
                     /gene="tcdA"
                     /locus_tag="KPE55_002905"
                     /gene_synonym="toxA"
     CDS             <1..8089
                     /gene="tcdA"
                     /locus_tag="KPE55_002905"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG0493949.1"
                     /translation="IRPRENEYKTILTNLDEYNKLTTNNNENKYLQLKKLNESIDVFM
                     NKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWIGGEVSDIALEYIKQW
                     ADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEIQNPQFDNMKFYKKRM
                     EFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDETVLESYRTNSLRKINS
                     NHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLALKNFGGVYLDVDMLPG
                     IHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENFDKLDQQLKDNFKLII
                     ESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGSYLTNLVIEQVKNRYQ
                     FLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIAPYLQVGFMPEARSTI
                     SLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLSQLTEQEINSLWSFDQ
                     ASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKIPSNNVEEAGSKNYVH
                     YIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLSDDGESILELNKYRIP
                     ERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLDTIKLDISPKNVEVNL
                     LGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIGANQYEVRINSEGRKE
                     LLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPGLASISEDIKTLLLDA
                     SVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDDLIDEFNLLENVSDEL
                     YELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVETEKEIFSKYSEHITK
                     EISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLIDYSSNKDVLNDLSTSV
                     KVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEGIPIVSTILDGINLGA
                     AIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIGAEVTIFLLPIAGISA
                     GIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKILVPIDDLVISEIDFNN
                     NSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSIYSAIGIETENLDFSK
                     KIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPGKFYWRFYAFFDYAIT
                     TLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGAGGTYSLLLSSYPIST
                     NINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDINKNKLIIGNQTIDFS
                     GDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNYLISNLSNTIEKINTL
                     GLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILEFYNDSTLEFNSKDFI
                     AEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKVNGLYLNESVYSSYLD
                     FVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTLVGKTNLGYVEFICDN
                     NKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDISTSLDFSYEPLYGIDR
                     YINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKVNINLDSSSFEYKWST
                     EGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKDIKKLSLGYIMSNFKS
                     FNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFYFDPIEFNLVTGWQTI
                     NGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPDGFEYFAPANTQNNNI
                     EGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNN
                     KYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFS
                     TSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNSKAVTGWQTIDSKKYY
                     FNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAIASTGYTIIN
                     GKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILYQNEFLTLNGKKYYFG
                     SDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGYITIERNNF
                     YFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDND
                     SKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYY
                     FNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAIL
                     YQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNT
                     NTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQN
                     RFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLP
                     QIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTI
                     NGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(8424..9122)
                     /gene="tcdC"
                     /locus_tag="KPE55_002906"
     CDS             complement(8424..9122)
                     /gene="tcdC"
                     /locus_tag="KPE55_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG0493950.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         3536 a         1145 c         1336 g         3107 t
ORIGIN      
        1 cattagacca agagaaaatg agtataaaac tatactaact aatttagacg aatataataa
       61 gttaactaca aacaataatg aaaataaata tttacaatta aaaaaactaa atgaatcaat
      121 tgatgttttt atgaataaat ataaaacttc aagcagaaat agagcactct ctaatctaaa
      181 aaaagatata ttaaaagaag taattcttat taaaaattcc aatacaagcc ctgtagaaaa
      241 aaatttacat tttgtatgga taggtggaga agtcagtgat attgctcttg aatacataaa
      301 acaatgggct gatattaatg cagaatataa tattaaactg tggtatgata gtgaagcatt
      361 cttagtaaat acactaaaaa aggctatagt tgaatcttct accactgaag cattacagct
      421 actagaggaa gagattcaaa atcctcaatt tgataatatg aaattttaca aaaaaaggat
      481 ggaatttata tatgatagac aaaaaaggtt tataaattat tataaatctc aaatcaataa
      541 acctacagta cctacaatag atgatattat aaagtctcat ctagtatctg aatataatag
      601 agatgaaact gtattagaat catatagaac aaattctttg agaaaaataa atagtaatca
      661 tgggatagat atcagggcta atagtttgtt tacagaacaa gagttattaa atatttatag
      721 tcaggagttg ttaaatcgtg gaaatttagc tgcagcatct gacatagtaa gattattagc
      781 cctaaaaaat tttggcggag tatatttaga tgttgatatg cttccaggta ttcactctga
      841 tttatttaaa acaatatcta gacctagctc tattggacta gaccgttggg aaatgataaa
      901 attagaggct attatgaagt ataaaaaata tataaataat tatacatcag aaaactttga
      961 taaacttgat caacaattaa aagataattt taaactcatt atagaaagta aaagtgaaaa
     1021 atctgagata ttttctaaat tagaaaattt aaatgtatct gatcttgaaa ttaaaatagc
     1081 tttcgcttta ggcagtgtta taaatcaagc cttgatatca aaacaaggtt catatcttac
     1141 taacctagta atagaacaag taaaaaatag atatcaattt ttaaaccaac accttaaccc
     1201 agccatagag tctgataata acttcacaga tactactaaa atttttcatg attcattatt
     1261 taattcagct accgcagaaa actctatgtt tttaacaaaa atagcaccat acttacaagt
     1321 aggttttatg ccagaagctc gctccacaat aagtttaagt ggtccaggag cttatgcgtc
     1381 agcttactat gatttcataa atttacaaga aaatactata gaaaaaactt taaaagcatc
     1441 agatttaata gaatttaaat tcccagaaaa taatctatct caattgacag aacaagaaat
     1501 aaatagtcta tggagctttg atcaagcaag tgcaaaatat caatttgaga aatatgtaag
     1561 agattatact ggtggatctc tttctgaaga caatggggta gactttaata aaaatactgc
     1621 cctcgacaaa aactatttat taaataataa aattccatca aacaatgtag aagaagctgg
     1681 aagtaaaaat tatgttcatt atatcataca gttacaagga gatgatataa gttatgaagc
     1741 aacatgcaat ttattttcta aaaatcctaa aaatagtatt attatacaac gaaatatgaa
     1801 tgaaagtgca aaaagctact ttttaagtga tgatggagaa tctattttag aattaaataa
     1861 atataggata cctgaaagat taaaaaataa ggaaaaagta aaagtaacct ttattggaca
     1921 tggtaaagat gaattcaaca caagcgaatt tgctagatta agtgtagatt cactttccaa
     1981 tgagataagt tcatttttag ataccataaa attagatata tcacctaaaa atgtagaagt
     2041 aaacttactt ggatgtaata tgtttagtta tgattttaat gttgaagaaa cttatcctgg
     2101 gaagttgcta ttaagtatta tggacaaaat tacttccact ttacctgatg taaataaaaa
     2161 ttctattact ataggagcaa atcaatatga agtaagaatt aatagtgagg gaagaaaaga
     2221 acttctggct cactcaggta aatggataaa taaagaagaa gctattatga gcgatttatc
     2281 tagtaaagaa tacatttttt ttgattctat agataataag ctaaaagcaa agtccaagaa
     2341 tattccagga ttagcatcaa tatcagaaga tataaaaaca ttattacttg atgcaagtgt
     2401 tagtcctgat acaaaattta ttttaaataa tcttaagctt aatattgaat cttctattgg
     2461 tgattacatt tattatgaaa aattagagcc tgttaaaaat ataattcaca attctataga
     2521 tgatttaata gatgagttca atctacttga aaatgtatct gatgaattat atgaattaaa
     2581 aaaattaaat aatctagatg agaagtattt aatatctttt gaagatatct caaaaaataa
     2641 ttcaacttac tctgtaagat ttattaacaa aagtaatggt gagtcagttt atgtagaaac
     2701 agaaaaagaa attttttcaa aatatagcga acatattaca aaagaaataa gtactataaa
     2761 gaatagtata attacagatg ttaatggtaa tttattggat aatatacagt tagatcatac
     2821 ttctcaagtt aatacattaa acgcagcatt ctttattcaa tcattaatag attatagtag
     2881 caataaagat gtactgaatg atttaagtac ctcagttaag gttcaacttt atgctcaact
     2941 atttagtaca ggtttaaata ctatatatga ctctatccaa ttagtaaatt taatatcaaa
     3001 tgcagtaaat gatactataa atgtactacc tacaataaca gaggggatac ctattgtatc
     3061 tactatatta gacggaataa acttaggtgc agcaattaag gaattactag acgaacatga
     3121 cccattacta aaaaaagaat tagaagctaa ggtgggtgtt ttagcaataa atatgtcatt
     3181 atctatagct gcaactgtag cttcaattgt tggaataggt gctgaagtta ctattttctt
     3241 attacctata gctggtatat ctgcaggaat accttcatta gttaataatg aattaatatt
     3301 gcatgataag gcaacttcag tggtaaacta ttttaatcat ttgtctgaat ctaaaaaata
     3361 tggccctctt aaaacagaag atgataaaat tttagttcct attgatgatt tagtaatatc
     3421 agaaatagat tttaataata attcgataaa actaggaaca tgtaatatat tagcaatgga
     3481 ggggggatca ggacacacag tgactggtaa tatagatcac tttttctcat ctccatctat
     3541 aagttctcat attccttcat tatcaattta ttctgcaata ggtatagaaa cagaaaatct
     3601 agatttttca aaaaaaataa tgatgttacc taatgctcct tcaagagtgt tttggtggga
     3661 aactggagca gttccaggtt taagatcatt ggaaaatgac ggaactagat tacttgattc
     3721 aataagagat ttatacccag gtaaatttta ctggagattc tatgcttttt tcgattatgc
     3781 aataactaca ttaaaaccag tttatgaaga cactaatatt aaaattaaac tagataaaga
     3841 tactagaaac ttcataatgc caactataac tactaacgaa attagaaaca aattatctta
     3901 ttcatttgat ggagcaggag gaacttactc tttattatta tcttcatatc caatatcaac
     3961 gaatataaat ttatctaaag atgatttatg gatatttaat attgataatg aagtaagaga
     4021 aatatctata gaaaatggta ctattaaaaa aggaaagtta ataaaagatg ttttaagtaa
     4081 aattgatata aataaaaata aacttattat aggcaatcaa acaatagatt tttcaggcga
     4141 tatagataat aaagatagat atatattctt gacttgtgag ttagatgata aaattagttt
     4201 aataatagaa ataaatcttg ttgcaaaatc ttatagtttg ttattgtctg gggataaaaa
     4261 ttatttgata tccaatttat ctaatactat tgagaaaatc aatactttag gcctagatag
     4321 taaaaatata gcgtacaatt acactgatga atctaataat aaatattttg gagctatatc
     4381 taaaacaagt caaaaaagca taatacatta taaaaaagac agtaaaaata tattagaatt
     4441 ttataatgac agtacattag aatttaacag taaagatttt attgctgaag atataaatgt
     4501 atttatgaaa gatgatatta atactataac aggaaaatac tatgttgata ataatactga
     4561 taaaagtata gatttctcta tttctttagt tagtaaaaat caagtaaaag taaatggatt
     4621 atatttaaat gaatccgtat actcatctta ccttgatttt gtgaaaaatt cagatggaca
     4681 ccataatact tctaatttta tgaatttatt tttggacaat ataagtttct ggaaattgtt
     4741 tgggtttgaa aatataaatt ttgtaatcga taaatacttt acccttgttg gtaaaactaa
     4801 tcttggatat gtagaattta tttgtgacaa taataaaaat atagatatat attttggtga
     4861 atggaaaaca tcgtcatcta aaagcactat atttagcgga aatggtagaa atgttgtagt
     4921 agagcctata tataatcctg atacgggtga agatatatct acttcactag atttttccta
     4981 tgaacctctc tatggaatag atagatatat caataaagta ttgatagcac ctgatttata
     5041 tacaagttta ataaatatta ataccaatta ttattcaaat gagtactacc ctgagattat
     5101 agttcttaac ccaaatacat tccacaaaaa agtaaatata aatttagata gttcttcttt
     5161 tgagtataaa tggtctacag aaggaagtga ctttatttta gttagatact tagaagaaag
     5221 taataaaaaa atattacaaa aaataagaat caaaggtatc ttatctaata ctcaatcatt
     5281 taataaaatg agtatagatt ttaaagatat taaaaaacta tcattaggat atataatgag
     5341 taattttaaa tcatttaatt ctgaaaatga attagataga gatcatttag gatttaaaat
     5401 aatagataat aaaacttatt actatgatga agatagtaaa ttagttaaag gattaatcaa
     5461 tataaataat tcattattct attttgatcc tatagaattt aacttagtaa ctggatggca
     5521 aactatcaat ggtaaaaaat attattttga tataaatact ggagcagctt taactagtta
     5581 taaaattatt aatggtaaac acttttattt taataatgat ggtgtgatgc agttgggagt
     5641 atttaaagga cctgatggat ttgaatattt tgcacctgcc aatactcaaa ataataacat
     5701 agaaggtcag gctatagttt atcaaagtaa attcttaact ttgaatggca aaaaatatta
     5761 ttttgataat gactcaaaag cagtcactgg atggagaatt attaacaatg agaaatatta
     5821 ctttaatcct aataatgcta ttgctgcagt cggattgcaa gtaattgaca ataataagta
     5881 ttatttcaat cctgacactg ctatcatctc aaaaggttgg cagactgtta atggtagtag
     5941 atactacttt gatactgata ccgctattgc ctttaatggt tataaaacta ttgatggtaa
     6001 acacttttat tttgatagtg attgtgtagt gaaaataggt gtgtttagta cctctaatgg
     6061 atttgaatat tttgcacctg ctaatactta taataataac atagaaggtc aggctatagt
     6121 ttatcaaagt aaattcttaa ctttgaatgg taaaaaatat tactttgata ataactcaaa
     6181 agcagttacc ggatggcaaa ctattgatag taaaaaatat tactttaata ctaacactgc
     6241 tgaagcagct actggatggc aaactattga tggtaaaaaa tattacttta atactaacac
     6301 tgctgaagca gctactggat ggcaaactat tgatggtaaa aaatattact ttaatactaa
     6361 cactgctata gcttcaactg gttatacaat tattaatggt aaacattttt attttaatac
     6421 tgatggtatt atgcagatag gagtgtttaa aggacctaat ggatttgaat attttgcacc
     6481 tgctaatacg gatgctaaca acatagaagg tcaagctata ctttaccaaa atgaattctt
     6541 aactttgaat ggtaaaaaat attactttgg tagtgactca aaagcagtta ctggatggag
     6601 aattattaac aataagaaat attactttaa tcctaataat gctattgctg caattcatct
     6661 atgcactata aataatgaca agtattactt tagttatgat ggaattcttc aaaatggata
     6721 tattactatt gaaagaaata atttctattt tgatgctaat aatgaatcta aaatggtaac
     6781 aggagtattt aaaggaccta atggatttga gtattttgca cctgctaata ctcacaataa
     6841 taacatagaa ggtcaggcta tagtttacca gaacaaattc ttaactttga atggcaaaaa
     6901 atattatttt gataatgact caaaagcagt tactggatgg caaaccattg atggtaaaaa
     6961 atattacttt aatcttaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa
     7021 aaaatattac tttaatctta acactgctga agcagctact ggatggcaaa ctattgatgg
     7081 taaaaaatat tactttaata ctaacacttt catagcctca actggttata caagtattaa
     7141 tggtaaacat ttttatttta atactgatgg tattatgcag ataggagtgt ttaaaggacc
     7201 taatggattt gaatactttg cacctgctaa tacggatgct aacaacatag aaggtcaagc
     7261 tatactttac caaaataaat tcttaacttt gaatggtaaa aaatattact ttggtagtga
     7321 ctcaaaagca gttaccggat tgcgaactat tgatggtaaa aaatattact ttaatactaa
     7381 cactgctgtt gcagttactg gatggcaaac tattaatggt aaaaaatact actttaatac
     7441 taacacttct atagcttcaa ctggttatac aattattagt ggtaaacatt tttattttaa
     7501 tactgatggt attatgcaga taggagtgtt taaaggacct gatggatttg aatactttgc
     7561 acctgctaat acagatgcta acaatataga aggtcaagct atacgttatc aaaatagatt
     7621 cctatattta catgacaata tatattattt tggtaataat tcaaaagcgg ctactggttg
     7681 ggtaactatt gatggtaata gatattactt cgagcctaat acagctatgg gtgcgaatgg
     7741 ttataaaact attgataata aaaattttta ctttagaaat ggtttacctc agataggagt
     7801 gtttaaaggg tctaatggat ttgaatactt tgcacctgct aatacggatg ctaacaatat
     7861 agaaggtcaa gctatacgtt atcaaaatag attcctacat ttacttggaa aaatatatta
     7921 ctttggtaat aattcaaaag cagttactgg atggcaaact attaatggta aagtatatta
     7981 ctttatgcct gatactgcta tggctgcagc tggtggactt ttcgagattg atggtgttat
     8041 atatttcttt ggtgttgatg gagtaaaagc ccctgggata tatggctaaa atatatgttt
     8101 gataaaaaat tattcctgtg ctactaagaa attattttta tataataaat attgagattt
     8161 aattaaaagt catgtgttat tgtaatacat gacttttagt taaaattttt ctatcattta
     8221 ataatctatt attcttgact attttataat aaaattcata tatggaaata ttaatactaa
     8281 ataattaata gttgataaaa aatagataat atgctaaaag caaaaactaa tttagagcct
     8341 tgtaactgtt tatttgcaat tataaaaaca tctttaaaca tattgactat aatataaaat
     8401 attaactata atacaaaaca atattaatta attttctcta cagctatccc tggtatagtt
     8461 atatttccac ccatagttga ttcataagtt atagttccag cacttatacc tcttatagtt
     8521 atgtaatcat cctctaatac tcttgaagga gttattgatt ttttataact acatagtaca
     8581 accttatcat aatttccaga cacagctaat cttatttgca cctcatcacc atcttcaata
     8641 acttgaataa ccttaccttc aaattttacc tttttgtact tataatcatc aggtgttcta
     8701 gctaattggt cataagtaat accagtatca tatcctttct tctcctcttc ttcttttttg
     8761 cgttgttctt cagccttttt agcttcttca gcttttttag cttcttcagc tttacgttga
     8821 ttttcagctt caatagcttt cttttcgtcg tctttcattt tgaaccatgg ttcagcatca
     8881 gacaatttgc tatttaaagt ttctatttct gttttttgag aatctataac tgatgtttgg
     8941 ttagaaatga cctcctcatg gtcttcagaa caagctggtg aggatatatt gccaaaaaac
     9001 acgcctaaaa taaatgccag tagagcaata ccctttgtgc tcttaaagaa tttaattatt
     9061 ttcttagagc ttccttttcc ttcattacta aattcgttac cctcattttt tttagaaaac
     9121 ataa
//
LOCUS       DAELNY010000152         9107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.154, whole genome shotgun sequence.
ACCESSION   DAELNY010000152 DAELNY010000000
VERSION     DAELNY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.154"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..596
                     /locus_tag="KPE55_002907"
     CDS             39..596
                     /locus_tag="KPE55_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0493951.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSKKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(835..1695)
                     /locus_tag="KPE55_002908"
     CDS             complement(835..1695)
                     /locus_tag="KPE55_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0493952.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            2193..3596
                     /locus_tag="KPE55_002909"
     CDS             2193..3596
                     /locus_tag="KPE55_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493953.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESKIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGYKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            3815..6292
                     /locus_tag="KPE55_002910"
     CDS             3815..6292
                     /locus_tag="KPE55_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0493954.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            6307..6993
                     /locus_tag="KPE55_002911"
     CDS             6307..6993
                     /locus_tag="KPE55_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493955.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKDVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TIIVSDGLIV"
     gene            7267..8100
                     /locus_tag="KPE55_002912"
     CDS             7267..8100
                     /locus_tag="KPE55_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0493956.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            8138..8599
                     /locus_tag="KPE55_002913"
     CDS             8138..8599
                     /locus_tag="KPE55_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0493957.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRFLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            8630..8878
                     /locus_tag="KPE55_002914"
     CDS             8630..8878
                     /locus_tag="KPE55_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0493958.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         3492 a         1026 c         1353 g         3236 t
ORIGIN      
        1 acagtatatt tagattaatg tataaagggg gattagaaat gtctgattct gaatatcctc
       61 aaatgaaaac cttagaaact agaagttgta tactaagacc tgcatcatta gatgatgttg
      121 aagatttttt tgactgttat aaaaataaaa tagtagtaaa acatttacca tttagtgagc
      181 ataaatcttt agaagataca agaaagttta taaaatcatt ttttctaaat ccatataaaa
      241 aagggaaaat tggccacttt gctatagtat ataaaagaga caataaagtt attgggaata
      301 tggggtttaa taatataaat cctaaagcat tagaagcaga aataggtata tgtataaatc
      361 cgagctattg gggacatgat tttgctactg agataacaaa aagagtaata cagtatggat
      421 ttagagaact aaacttaaat aaaataattg caataaccta tgaagaaaac caaaattcta
      481 aaaaatcatt agatttatta ggatttaaat gtacaggaaa gtatatcaaa aaaattcata
      541 ctggaaatac tgtaaaaaat gttccttgtt atcaatatga attgaaaaaa caatagatat
      601 aatcatttaa tatctatgct ggttatttag taatgattgt tttctaaata aaatttcaat
      661 aaagtggttg cctcaaaata tagataatcc tgtttttatt gttaaaatac tgaaaatgca
      721 tttgatttta tacaaaaaag agtgtctcat gaactaaaaa gtttatgaga cactcttttt
      781 gaataaatcg tattttaatt aaaatagtat atatacttaa aatattaatt tttattagtt
      841 ctctaaattt ttaaatcctt ctccaagaac ctcatgtaca tctgtaacaa taacaaatgc
      901 agtagggtca gttcttttaa ctaatctctt tagataaagt tcttgttttt tagaaactac
      961 gactaataga acttctttat tttctctagt gtatccacct ttaccatcaa gaatagtaag
     1021 acctctatca agttcttcag ttatagcttg tcttaaactt tcaggttcat cagagataat
     1081 aaaaaatgct tttgaatggt caaatccttc cattatcata tctgctattt ttattaatac
     1141 acaaagtgct ataccagagt aaagagcagt ttctaaacta cgatttacta taccagaaga
     1201 tataacaacc attccatcta ggcatgacat gaattttgta gcttttattc ctgggaattt
     1261 cttactaagt atgagtgcaa ttaagtctgt tcctccagta gaagcatctg atttaaacat
     1321 tattcctaaa cctataccaa ctaggattcc tcctgcaata gaagacaata ataagtcatt
     1381 agtaaatctt agttttgata atgggtctgt taattgaact atagctgaaa atacaactgt
     1441 tccaaataaa gtttttaatg aattttttgt acccataatt ctaaaagcaa gtattactaa
     1501 tgggacacct ataattaaca tgatagctga tactggaagt cctgtaacct tgtttagtac
     1561 taatgataga ccactaagtc ctccaggagc aattgtatgt ggttttaaaa acatatttat
     1621 accaatactc attgatatac aaccaaaaaa tagtccaagc atttcaagta aaattggcga
     1681 cttttgtttt ttcatataaa cacctccatg aacttgtcac aatttaatat tataacatat
     1741 ttgaccagtt tatattatat aaaatttctt gatataagca atttcaagta tttcattatt
     1801 cttaatattt ttgaattttt atatagtgtt ttcatatttt tactgatgtt gttacactcc
     1861 tatgaagttt ttatatatta taattttttt attcttttat aaagtatccc ctataaagat
     1921 ggttctatga aaattttctc tatactgttt aacgtatata gagattttgt gtataattat
     1981 aaggtattta atggaaaaat ttatcttact tttatatgtt atcaaaatga tttattaaat
     2041 acaacagaca gcttgataac atataattat ataaatataa tttatgtaga tagttgtatt
     2101 tttaaataaa tttttacata tcaataatta ttgtctattt tatataagca acaatcaaac
     2161 taaaataaaa gtttaagaaa agagttgatt aaatgcataa acctagttat aaaacagtat
     2221 ttcattcata cattattttt ttcatgattt ttatttgctt aatcctgaca gtaataggta
     2281 ttttcttatc acttattact attcagaaac caaatggtga atttattcgt agtgattggc
     2341 cgaaaaaatt tacagaaaac tttaatgaac aaatcgtatt tatagataat aagccacaag
     2401 taaaacagtc tggattagaa aaacttcgag aaaatcaatt gtggttgcaa attttagatt
     2461 ctagtggaaa tagagtttat gggtttttag aaccaaaaga gcataaagat tattattcta
     2521 attctgaatt attagcatta gctaaccaaa ataattcaag taatggaact gtatacttta
     2581 atacaattaa aaataataaa aatgactata tttatattat atattttcca ttaaatatat
     2641 ctaaagtgaa tatgtttttt gatggaggta aatttatagg tggtaagtca attgtatttt
     2701 caatcatagg gattatattt ttaataattt tggtttctgg aattgcttat ggttactggg
     2761 taacaaagtg catatcaaat attacatctg ctgttagcga tatttcaaaa cgaagctatc
     2821 ttccagttaa tattaaaggt gtttttgctg atatatataa tagtttaaat attttaaatg
     2881 aagaaattca taaaagtgat gagatacaga ataaaactga tattatgaga gaagagtgga
     2941 tttcaaatat aactcatgat ttaaaaactc cactttcacc aataaaaggg tatgcagagc
     3001 ttcttacaga tagtagtact aattattcac ctgaaaaagt atataaatat gcttcaatca
     3061 tgttaaagaa catagaatat acaaatacac ttataaacga tttaaaatta acctatcaat
     3121 taaaaaatgg aataatccct ttaaataaaa ataattatga tttagtaaga tttttgaaag
     3181 aattatccat tgatatttta aattatccag aatatgaatc aaaaatagtt gaatttaaga
     3241 gtaatataga taatattaac tttgattttg attgtacact attaaaacga gcttttagta
     3301 atcttattgt caatgctttt acatatggat ataaaaatac taaagtttgt gtacatataa
     3361 cattaaatga aagaattgaa atcattatat ctgataatgg aaaagggatg tcaagagaag
     3421 aagcatcaaa tttgtttaat cgttattata gaggaagcaa tacaaaacaa aaaatagaag
     3481 gaactggttt aggactagca ataacgaagc aaattattga agttcatgga gggaatatat
     3541 ctgttgaaag tatacctgga ttaggtagct cattttatat cacctttcct atttaattaa
     3601 ggttaattaa ggtcaaataa aaaatatatt aaggttgata gattattata tttatatagt
     3661 agtttatcaa tcttttttat ttaaggggtg atttgattaa aaatattata aatataatga
     3721 taaaaatatg gaggagtcta ttagagattt aatttagatt aaagtataaa aatattagat
     3781 tgaaatatct tagataaggg aagaaggtgt aagtttgaaa attatattaa agtatatttt
     3841 cacaaatatt aaagaacgaa aaattagaac agcagttatg ttattgtcca tagttttatc
     3901 aacagtttta ctttttgttt cattctcaat tggattatct tatgaaagtg cacaaagaaa
     3961 aatggcaaaa ggtatgtatg gtacagcgac tatatctgtc caaagtaaaa atccagatat
     4021 attaaccaat ttagaagaca tacctgattt aaatactata aaatcaaagg ttggtgtatt
     4081 ggaaagctct gctatttata ataaaggggg atactatgaa gaatttagtc ttatatcagc
     4141 agacttaagt caactaaata aaatcaacaa gccacgatta gaaaatggcg atagtattac
     4201 tgatttttct ggtgataaaa ttattcttcc aaatcgcttt acatcaaaat ataaaataaa
     4261 aaaaggtgat tctattactt tacagattta tggaaagtcc tatacttttc aagttagtga
     4321 tattgcatct tatgatacag tatttttgag gaacacaaga ggtgtaaatg cactgctacc
     4381 caaagagact ctatcaaaaa ttataaataa aggcagtgga tatacaagag ttcttataga
     4441 gtcagaagaa gaaattacag aaaatctagt aaataaatta tccgaagaac tttctactga
     4501 aaaatatatg gtatcaaata ctataaatga aactaagata atttcagatg ctagacaaaa
     4561 gacaatgcct ttctttttaa taagtttttt tgcattaacc cttagtatat ttataatata
     4621 cagcagttat aaggttatta cattagaacg tcttcctttt attggaacat ttagaagtat
     4681 tggtgccaat gaaaaaactg taactcgtat attgatgctt gaaagtatat tatacggaag
     4741 tataggagga ttaattgcta ttcctattgg tgtagttgtt ttaaatttaa tgcttcatgg
     4801 gttaggaaat tctctcgaac aaggaatatc aattccagta gtaatatcac ccattggagt
     4861 gattatatct gttatagtag caataatcgt ttcttcattt agtgcatata tacctgttaa
     4921 aaaagctagc catttgccga taaaaaatat tgtactagga acagtagaag aaaagaatgt
     4981 ttcaaatcgt tctatacttt ttataggaag tataatgttt attttatcta ttttacttcc
     5041 aagaatatcc cctgaaaata cattgtatct agctggtgga ttttcattac tgggattaat
     5101 tgtagctact attgtgttga ttcctcttat tactgatatc atgtctatag tttttgagtt
     5161 tgtttataaa aatattttag ggaatgaggg taaattagct gctagaaata tgaaaaataa
     5221 caaaaatata atacagaata tcactttact atttataagt atttctgcag ttattgcaat
     5281 aagtgttgtt ggtaactttg tcaaaactta tataacagat gtgtttcgtg acgcagaatt
     5341 acaaggtttt gcagatggaa agatgaatga agagtttatt gaagatgtta gacatatgga
     5401 tggaattaaa aaaatattac ctctttatgt tatgaataat gagatttctg gtaatggtgt
     5461 aacattatca agactagagg gtacagataa tataaaacta tataactcta tgtttggaat
     5521 caattataca aattttgaga taaaaaaaca agtaatagaa gcttttaatg ataaacgttc
     5581 tgttattttg aatgaagata ctctaaaaaa agtaggctta tctataggag atacaataac
     5641 tctatctaat gataagtatg atttttctta taaaatagta ggtagtttta aatcccgtgc
     5701 aaatgatgta gaagctgtta taccatcaca ttatgcagtt aatgattttg ataaaactaa
     5761 ttatggtttt ttagtatata cagcagtcaa tccagatgct gttatgattc agataagata
     5821 tttatttgga gatacatata attggagtcg tacagttgaa gaatttaata atgattcttt
     5881 aaatactata agtagttttt tgtctcctat gaataagatg acctatttca tttttcttct
     5941 tgctacagta ggtattatta ataatctact gattaattat attcaaaaaa gacgttctat
     6001 agctatgtat aaatctatag gactaagtga taaacaaaat ataaaagtaa cattgattga
     6061 agggtttact tcaggtttac ttggagctgt tattggtatt gtgatatcta tacttgagat
     6121 tcaaactatc tttatagttg caggaccaaa aatttcaatg aaaccagatt tagattttaa
     6181 aacatttatt attgttgggt tattgggaat tatagtaact ttaataggtt cgatagtgcc
     6241 tattataaaa gggaagaaaa tgaaactgat agaagaaatt aagtttgaat aattatggag
     6301 gttaatatga aaattacaca taatagttta gctgtagaag caaaaaatat tatcaaagaa
     6361 tataaaattg gaaatacaac aactcgagtg ttaaaagatg tatctttaca agtaatgaaa
     6421 ggtgaatttg tttctatcat gggtcaatct ggttctggca aaagtactct tttatatatt
     6481 ttaggtggtt tagatactcc aacgagcgga aaagtgtata tgaatggagc tgatatatca
     6541 cattttaatg atgagaaaat gagcataata cgtcgtagaa atataggatt cgtatttcaa
     6601 ttttataact taataccaaa tttaaatgtg gaggaaaata taatgcttcc tttgttatta
     6661 gatggtaaaa atttaaaaga ttataaaaat caattagatg aaatcttaga tatagttggt
     6721 ttaactgata gacgtaaaca tacaccacga gagttatctg gtggtcagca acaacgtgtt
     6781 gctatagctc gagctttaat tggaaaacca gagattttat ttgctgatga gcctactgga
     6841 aatttagata gtaaaacagg gatagaaata atagatttac tgaataaaat aaacagagac
     6901 aatggacaaa ctataattat ggtaactcac tcaccagaag cagccaaaag tagtagtcgt
     6961 actattatag tttcagatgg tttaattgta taaggactaa ttattgtatt tgagataagc
     7021 atttttaatt atataaatta gacatatgta aagtggatat aagttaaata ctataagtgt
     7081 gctataatat taaaataaca gataaaagta ctatattttt tattgattat tttaatttta
     7141 tttgtttatt aaatatctaa tgagtattca tactatctct attattaaaa gcagttaatg
     7201 atatatagag ataatatgga ttcttcttac tttaataaat attcacgata ggacaggata
     7261 taatatatgt taaaaaaaga aagtattcat tttataaata aaaataggtc acttatagtt
     7321 tgtgaagaca attttgttta tctcttgcct tgtatacaat taagaaattg gatatcgaat
     7381 tttactatat cgtttccaaa tcaaacaata atttctgata attacacgat tattcctcat
     7441 ggaagtgtaa ctttggtgtt tttttatgat gccacagggt tacatagtct attatttggt
     7501 ccaactacta aaccaaaaac agtaggaaat attgctaata gatgtgatgt tatactaatt
     7561 atagaatttc aacctgctgg ttttttcccg cttataggta tacagcaaag tgaacttatt
     7621 gataaagttg ttccattttc tataattaat acttcattag atttagaaat taaaaaaata
     7681 tttaatgaat ctttatctat agataaattg attttaaagt tggaagaatt attgatttct
     7741 aatattaaaa ttgaatactc atatgagttt attttggcaa ttcagttgat tattcaaaac
     7801 tctggaaata tatcttcaca agaaatttct aaaaaagtat tttatagttc taggcattta
     7861 aacagactct ttaatcagta cctagggttg agtatgaagt cattttctcg tttggtacgt
     7921 attaataaat ctataaaact tctaaacaat aataaaactt cattgatgtc tatatgtaat
     7981 gaattaggat tctatgatat accacatttt attaaagatt ttaaaattgt ctgtggaata
     8041 actcctcaaa aataccgagc taatatgtct gatttttaca gtgaaattgc aaaattttaa
     8101 gttataatac ttataacaaa gaaaggatag tgataagatg aaatttggag gagtattgct
     8161 aattgtcaaa gacataaata aatcaaaaaa gttttatgaa gatgttatgg agcaaaaaat
     8221 cacattagat ttaggagaac atgtatcatt tgaaaatgga tttggattac aaagtaatta
     8281 taaagctata attggatatg atttaccaat atatcaacaa tcaaataact ttcagttgta
     8341 ttttgaggtt aatgacatca aacaatggga aagtaagata aatagaatag gaaatattga
     8401 atttttgcat tatataaaag aatacccatg gggtcaaaga acttttaggt tttatgattt
     8461 tgataaaaat attatagaaa tttcagaaag tatggaaagc gtcataaaga ggtttttaaa
     8521 acaaggttta gctttagaag aaatttcaaa acgcactatg tatccagttg aatttattac
     8581 acaatttcaa caattataaa atattaaaat atataatttt ttctttatat tgcaaaaaac
     8641 aaaggcttgg ggcataatag aaccagaaaa acctgaatct caacagcaaa tggctcatat
     8701 ggatttcgct gttaatgatt tagaagaagc agttcaatat gcaattcatt gtggagctac
     8761 aatcgcagag gagcaattta ctgatgactg gagagttatg attgacccag ctggacaccc
     8821 tttttgctta tgtcagatga aatcaattat ggaaagtagc catttttcat tgttatagac
     8881 caaaatattg tttattaatt acaaaaaagc acaacagtat ataccgttgt gcttttactt
     8941 attatacatg aatacactaa aaaatataaa tagtagagct ttatcaataa tgataatttt
     9001 taaaaaatag tattttttta ttgaataatt tcttagatat tttaaagtat ataaattttg
     9061 catatatgaa ttggacatta tcaaatgtgt tataatatta aaacaac
//
LOCUS       DAELNY010000153         9045 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.155, whole genome shotgun sequence.
ACCESSION   DAELNY010000153 DAELNY010000000
VERSION     DAELNY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.155"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1127
                     /locus_tag="KPE55_002915"
     CDS             <1..1127
                     /locus_tag="KPE55_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG0493959.1"
                     /translation="RVKIVNLIDAVNKVVAEDIYSTIDSPPFDRSPYDGYAYNANSEN
                     RNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANCVIKKEDVTVIGDMITLNIK
                     LKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLGISKVTVYDDLKVGILNTGT
                     ELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNCSDDILDLEKKVKNMLQSVD
                     FLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGACYVGTLGEKLLFGLSGNPH
                     AAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSNKDRFVSGKLYTSEGKLYVE
                     LNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVIR"
     gene            1111..1683
                     /gene="mobB"
                     /locus_tag="KPE55_002916"
     CDS             1111..1683
                     /gene="mobB"
                     /locus_tag="KPE55_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG0493960.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            1802..2077
                     /locus_tag="KPE55_002917"
     CDS             1802..2077
                     /locus_tag="KPE55_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0493961.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            2332..4032
                     /locus_tag="KPE55_002918"
     CDS             2332..4032
                     /locus_tag="KPE55_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493962.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            4395..5582
                     /locus_tag="KPE55_002919"
     CDS             4395..5582
                     /locus_tag="KPE55_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493963.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            5642..6805
                     /locus_tag="KPE55_002920"
     CDS             5642..6805
                     /locus_tag="KPE55_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0493964.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPRILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            6956..7759
                     /gene="zupT"
                     /locus_tag="KPE55_002921"
     CDS             6956..7759
                     /gene="zupT"
                     /locus_tag="KPE55_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG0493965.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            7854..8414
                     /locus_tag="KPE55_002922"
     CDS             7854..8414
                     /locus_tag="KPE55_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG0493966.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            8664..>9045
                     /gene="rgbR"
                     /locus_tag="KPE55_002923"
     CDS             8664..>9045
                     /gene="rgbR"
                     /locus_tag="KPE55_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG0493967.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINK"
BASE COUNT         3489 a          898 c         1681 g         2977 t
ORIGIN      
        1 acagggtcaa gatagtaaat cttatagatg cagtaaataa ggtagttgca gaagatatat
       61 attcaacaat tgatagtcct ccatttgata ggtcacccta tgatggttat gcatataatg
      121 caaattctga aaatagaaat ttaaaggtta tagggacttt gtatgcagga gatgtattta
      181 atagggtatt aaataaaaat gaagcagtga agataatgac tggtggaaag ataccacagg
      241 gtgcaaattg tgtaataaaa aaagaagatg taacagttat aggtgatatg ataactttaa
      301 atataaaatt aaaggaatac gataattaca tatttaaggg tgaggatata aaaaaggggc
      361 aattgattat taataaaaat cagaatatag gatatgatgg tattggagta ttagcaagtc
      421 ttggaatatc caaagtaact gtttatgatg atttaaaagt tggaattttg aatacaggta
      481 cagaactaca agatataaat gagactttag agaatggtaa aatatacgat agtaatagat
      541 ataccttata ttcaagatta ttaaaacttg gaatacaagc agatacaata tgcaattgtt
      601 ctgatgatat attggattta gaaaaaaagg taaagaatat gttacagagt gtagattttt
      661 taattaccac aggtggtgtt tctgttggag ataaagattt aattccagat gtattcaaaa
      721 aaataggggc aacagaatta ttttggaaga taaacattaa acctggtgga gcttgttatg
      781 taggtacatt gggagaaaag ctattatttg gtctttctgg taatccacat gcagcaatag
      841 tggtatttga taatgtgata gttcctgtta ttgagtattt aacatataag aagggaacac
      901 atcatataaa agctatattt aaaggagaat ttaataaact ttcaaataaa gatagatttg
      961 taagtggaaa actatataca agtgaaggta aattgtatgt agagctaaat gataagaaag
     1021 atgcaaaagc tagactatct gcaactctaa gaagtaattg tatgattaag attaaaaaga
     1081 atgagacaat aaaagaaaat caattagtgg atgttgttat tagatagttt tatgaaagga
     1141 cacaatttta tgtataatat attgtcagtt gtctcatatt caggagtagg taaaactacg
     1201 ttgattgaag gcataataaa agaattaaat aaaaaagggt atcgtgttgg agttattaaa
     1261 catacctgtc atgattttga tatggatgag gaaggaaaag atacttataa acatagaaaa
     1321 tctggggcga gaaaagtctg tataatatca gataaacgag tagcatatat tgaagaacta
     1381 aaagagaaga atccattata taaaatgatt caattatatg aagatatgga cttgattatc
     1441 atagaaggat ataaaaatta tagatttaaa agattggaag taactagaaa aggtaagtat
     1501 gaggatattt tatctaataa gtctgatttg ataggtattg tcagtgatat agaatataaa
     1561 ttgaatattg aacagtttaa tttaaatgat tataaaaata tatcaaagta catagagtct
     1621 tgtataaaaa atgaaatttt aagtataagt aatgttgaaa taaaaaacat attaaaagaa
     1681 taattttaag atgttcttct tatataaaat gatataatta tttatataaa taaagatata
     1741 agaaatttat caattttatt ttgagttata aaatattaaa gaaattgtag gaggacaata
     1801 tatgaaaaaa atattagtag cctgtggagc aggaatcgca acatcaacaa tagtttgtga
     1861 cagagtagaa agattggtta aagaaaataa tatacaagca gaagttgagc aatgtaaaat
     1921 tgctgaatgt tcaacaaaac aagaaggtgc tgatttaatc gtatctacta ctatactacc
     1981 aacaacttat gatataccta caataaaggc aacaggatat ataacaggta taaatacaac
     2041 agcattagat aaaaagatat taaatgcatt gaagtagttt tgtatttata aaaaaaattt
     2101 agtattaaat atagagttgt atctttataa aattttaaat atagagatat agctcttttt
     2161 atgttgtttc aagtatacaa ctatatgata taaatcttca attttatgaa aaagattgtt
     2221 attactttgt aagtttataa aagatttaaa gttattgtta tgttatacta atttatgggt
     2281 attttttaga agtactaata gaagtactaa taaaggaggg atttaatact tatgataaaa
     2341 caatttgtca aatattataa gccatataag aaaattttta cactagacct tatagcagct
     2401 tttctatttt ccttatgtga tttagtttat cctatgataa ctagaaatat aatggatgac
     2461 gttgttccaa ataaaaactt aagaatgtta gtagtttttg cagtagcact tatacttata
     2521 tttatagcaa aagcagggct taactatttt atgcagtatt ggggacatgt tattggtgta
     2581 gatatgcaag ctgatatgag aaatgaggta tttacacact tacaaagact tccaaatacg
     2641 tattttgata ataacaaatc tggagtaact atgtctagaa tagtaaatga tttaatggat
     2701 ataacagaac ttgcacatca tgggcctgaa gatttattca tttctatagt aatgttggtt
     2761 ggttcattct ttatacttat agatataaat attcctctta cgcttataat atttgctata
     2821 ttaccgttta taatttggtt tgcaatagct aaaaaagata agatgaatat tgcttttatg
     2881 aagagtagag ttactatagg tgatgtaaat gctactttag aaaatagtat agcaggtatg
     2941 aaggtaacta agtctttttg tactgagaaa gaagagttaa ataagtttgt aagaagcaat
     3001 aaattattta gaagagcaag acaagattct tataaggtaa tggcagaata ttactctggt
     3061 atgaatttat acatggatat cttagagtgg gtagttgtaa tagctggtgg ttattttact
     3121 tatataggaa agattacttt gggtgatttt gctgcttata tactatatgt aaaaatgttt
     3181 atacaaccta tgaaaaaatt aataaacttt acagagcaat atcaaaatgg tatgacaggt
     3241 tttaaaagat tcatagagat aatggaacaa gaccatcaaa aagaagctaa aaatcctatt
     3301 gaacttgaaa atgtaaaagg tgacattgag atagaaaata tatctttcac atatgaagat
     3361 aagactcaag ttttggataa tttaagttta tctataaaag ctggtaagac tatagcatta
     3421 gttggacctt ctggaggagg aaagactaca ttatgtaatc tattgccaag attttatgaa
     3481 tttgataagg gtgatataaa aattgatgga aagagtataa aagatgttag tttaaaatca
     3541 ttgagaaaaa atataggtat agttcaacaa gatgtatttt tatttacagg aactataaga
     3601 gataatattc tttgtgggaa ccctaatgct acagatgaag aaatgatagc tgctgctaaa
     3661 aaagctagaa tacatgactt tgtagagact ttgccagatg gatatgatac ttatatagga
     3721 gaaagaggcg taaaattatc aggtgggcaa aaacaaagaa tttcaatttc aagaatcttt
     3781 ttaaagaatc ctccaataat aatattggat gaggctacat ctgctttaga caatgtaaca
     3841 gaaagagaaa ttcaagagtc tttagaggaa ttaagtaaag atagaacaaa tttagttgtt
     3901 gcacatagat tgactactat aaaaaatgct gatgaaatca tagtattgac agataaagga
     3961 attgaagaaa gaggtactca tgaagagcta gtaaacaaaa atggagtata tagtaggtta
     4021 cacaataact agtatacaaa ttttacacca tagctaacat acacattaaa cttacaaaca
     4081 taggatatat taagattcat aaagacaatt cattttatat ttataagatg ttttatgaat
     4141 aaaatgtatc ctatgtttta ttttttgcaa attacattta attctctttg taagaataga
     4201 gttaaatagc caatggtcgg gataacgttt ttacaaaaag aataatttag aaaactaact
     4261 ttaatttatg gaatttatcg aattttatga aaaaaatcga attttgactt gaaatgtttt
     4321 tcaaaagtag taaaataatg gataactata catacatttt ataaaaatat atttattatg
     4381 aggggggatt ttgtatggag gcaataacag ctttttgtct agttttagta tcactgacga
     4441 taggagatat agtttcagca aaaactaaag cttttgttcc gtcggttttt gtatcagcaa
     4501 ttatatttat agtaggattt tggacttttt tcccagaaaa tattgtggac ttagccgctt
     4561 taggaacacc attagctcaa ttaggtatgc ttttgttaat aactcatatg ggaactatga
     4621 tgagtattaa ggaattagca ggtcaatgga aaacaatagt aatagccttg gcaggaatag
     4681 taggaatatg tgtaggtgct ttggcattag ggacagtagt atttggatgg gatacagcag
     4741 taatagctac acctcctctt actggagggc tagttgcatc tataatgatg gcagatgctg
     4801 ctacagcaaa aggtcttgca agtttatcag tacttgcaat tttgatgtat gttgcacaag
     4861 gatttgcggg ttatcctatt acagcattaa tgcttaaaaa agaaggaaaa agacttttat
     4921 cagactttag aagtggaaaa gtaactgtaa atacagatga agaaaaagtt aaggatttac
     4981 ctgaacaaaa aagcagattt aagataattc cagacttacc agaaaaatat gatacgacat
     5041 atatgataat acttagatta ggatttgtag catggttagc agtacaattt acaaatctta
     5101 caaatgaagt tgtatcaaga tatgttatat gtttaatatt tggtgtaata gcatcagaac
     5161 ttggaatagt tgatagaaaa ccacttaata aatctggttc atttggatgg ttaatgacat
     5221 cattgatggc ttttatattt gcagggttag caaaagctac accagcaatg cttgttgaaa
     5281 tggcagttcc tcttgtagga atcataatat ttggtgtaat aggtatggga atattctcta
     5341 ttatagtagg taagctttta ggtgaaagta aagaaatggc tttttcagtt tcgcttacat
     5401 cactttatgg ttttccacca aactatatat taactgaaga ggcagtaaag gctttggctg
     5461 aaacaccaga agaaaaagaa tttttaatgg ataagatgct tccaaagatg cttgttggag
     5521 gatttactac agttacaata gcttctgtag taatagcggg tatctttgta aatctaatat
     5581 agtatttgag tgataaaaag tattttaaat aagaagaatg tgtactactt ggaggtatgg
     5641 aatggatatt aaagaaataa caaagagtta taaagactat gtaattaagc ttagaaggga
     5701 atttcatgaa aatcctgaga aaagtatgga agaagttaga acatcaaaga gagttaaaga
     5761 agaacttgat aaaataggga taccatatgt atcagctgga ggtacaggtg taattgcaac
     5821 tataaaagga gctaatccag gaaagactgt agcacttagg ggtgatatgg atgctctaca
     5881 agttgttgaa tgcactgatg tagaatacaa atctaaaaat gaaggactta tgcatgcttg
     5941 tggtcatgat ggtcacacat caatgcttct aggggcagca aaagtgttaa atgatattaa
     6001 ggatagtatc aatggaactg taaaattatt ttttcagcca ggagaagaag ttggtaaagg
     6061 tgcaagagct atgattcaag atggtgctat ggaaggtgta gatagtgtgt ttggaataca
     6121 tctttggact gatgtagaat ctgggaccat atcggtagaa gaaggaccaa gaatggcttc
     6181 tgctgatttc tttaaaatta ctgtaaaagg tagaggtggt catggttcac ttcctcatca
     6241 aggtgttgat gcagtgcttg caagttcagc aatagttatg aatctacaat ctatggttag
     6301 tagagaagta agtcctctgg aaccattagt tgtgagtgtt ggagttttaa attctggaac
     6361 tagatttaat gtaattgcga gtgaagctat cttagaaggt acaataaggc tatttaatcc
     6421 agagcttaga aagcaaattc ctagaatttt agaacgtata gcaaaatcta cagcagaagc
     6481 atatagagca gatgctgaat tagagtatgg gtacttaaca ccagcagtta taaatgacaa
     6541 agaatgttca aagatagcta cagatgcagc aattaagcta tttggagaag actgtataac
     6601 actatttgaa aaagtaacag gagcagaaga tttagctgag tttatgaata tagcacctgg
     6661 tgcactagca tttgtaggag ctagaaatga gtcaaaaggt gcttgttatc ctcatcatca
     6721 tggatgcttt aatatagatg aagatgctct agaaattgga actgctttat atgtgcaata
     6781 tgcagtggat ttcttaaata aataatttta atatactata aagaaaaagc agagactaag
     6841 tttatatctc tgctttttta ttgtgtatat tttttataaa tattgactat ataaaatata
     6901 tatgatagca taaagaaaat gataatcaat atcaattaaa aagaaggtga tttatttgaa
     6961 taatgtaata tttgcatttt tgataacatt attggctggt ttatctactg gtataggaag
     7021 ttgtatagca ttttttgcta aaaaaacaaa tactaagttt ctttctataa gcttaggatt
     7081 ttctgctggt gttatgattt atgtttctat gatagaaatt tttcctaaag cacaagatgc
     7141 tttgacaaaa tctatgggag agaaattagg aagttggtca actgtaatag ctttttttat
     7201 aggtatggct atcattgctg ttattgataa gctaattcca caagaagaaa atccacatga
     7261 aataaagaaa atggagaata tagaagaaaa gaatatcaaa aaaaataagt ctcttttaag
     7321 aacaggtata tttacagcaa tggctatagc aattcacaat tttcctgaag gattagctac
     7381 atttatatca gctcttgatg atgttacaat agctattccg atagcaattg caattgcaat
     7441 acacaatata ccagaaggta tatctgtatc tgtaccagtt tattatgcca caggagataa
     7501 aaagaaagct ttttattatt cctttttatc tggtatgtct gagcctttag gagcgattat
     7561 tggatatgta cttcttagaa attttttaaa tgatataact cttggaattg tatttgcaat
     7621 tgtaggagga attatggtat tcatatcact agatgaatta ttgccatcag ctagagagta
     7681 cggggaacat catttatcta tttatggatt gattgcagga atgggtgtta tggctattag
     7741 tttattacta tttaaataac ttttaaatat atgattgtaa cctaaaatta gtatcaaaat
     7801 gatataatta tttaaataga tttttaagct atttaaaaat atcattttgg agaatgagtg
     7861 ctatggaaaa gaaaataaaa ttcaaatttg actggtatag ttcacctgat aagatagaga
     7921 aatggttaga agatattgaa gctaaaggat ataatttata taagataaat aaatttggag
     7981 gaatatttta ttttcataag ggaagtccaa gaaaaataaa atacactgtt gattactact
     8041 atgatgtaga gaataattat tttgatgaac ataaatcaaa tggctggaat cttgtattta
     8101 ggagtattgg tagttttatg ggtacatctg catggtatat atggtcgaaa gaatacaaag
     8161 acatacctcc taaattatat agtgattctg ataaaacaaa taaagtaaag tcaattttat
     8221 ttatgaatat gtttatattc tttatgttag tagtaatatt tatatattta ttgaatattg
     8281 aaataagaaa tttatatagt ggttatggag catctgtaac tgctattgta ctacagatta
     8341 tattgttgat tttatatgca gtacaattat ataggagatt aatgtattat ttaaggctta
     8401 aaaagtttaa ataaataaaa gaaggtgtcc taaaattatt tttttaatat tttaagacat
     8461 cttcttttgt cagtaagtac tgtttttaat tatagtaatt tctgtcttta aattgaaata
     8521 tcaaataata gttgtatggg attgagtatt gataaaaata atttaaaaat gttatcctaa
     8581 agtatcagat atacaaaatt atctttaaat atattataaa tattttaatt gtattaacta
     8641 gaaattatgt aaggaggcat tttatgatta agatagcagt ttgtgaagat gaaaaagaaa
     8701 cacaactttt aatagaagat taccttgaga atatattaaa agatataagt atagaatatg
     8761 agatacaaaa gtatatatct ggagaagagt tactggaaag taatttaaaa gatatagata
     8821 tattacttct tgatataaaa atggaaaaat taaatggaat ggatactgca agaaagatta
     8881 gagaagtaga caatgaaatg gaaataatat ttgtaacttc attaatagat tatgtgcaag
     8941 aaggttatga ggtaagagct tataggtatt tactaaagcc aatagagttg gaagaactaa
     9001 aaaaacatgt gctaacttgt atcaaagata ttgaaataaa taagg
//
LOCUS       DAELNY010000154         9015 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.156, whole genome shotgun sequence.
ACCESSION   DAELNY010000154 DAELNY010000000
VERSION     DAELNY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.156"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            7..636
                     /locus_tag="KPE55_002924"
     CDS             7..636
                     /locus_tag="KPE55_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG0493968.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            875..2134
                     /locus_tag="KPE55_002925"
     CDS             875..2134
                     /locus_tag="KPE55_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0493969.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            2393..3388
                     /locus_tag="KPE55_002926"
     CDS             2393..3388
                     /locus_tag="KPE55_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG0493970.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            3634..4701
                     /locus_tag="KPE55_002927"
     CDS             3634..4701
                     /locus_tag="KPE55_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG0493971.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFVEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            5235..7145
                     /locus_tag="KPE55_002928"
     CDS             5235..7145
                     /locus_tag="KPE55_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0493972.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMLF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFVSVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(7208..7831)
                     /gene="ytaF"
                     /locus_tag="KPE55_002929"
     CDS             complement(7208..7831)
                     /gene="ytaF"
                     /locus_tag="KPE55_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG0493973.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            8019..8663
                     /locus_tag="KPE55_002930"
     CDS             8019..8663
                     /locus_tag="KPE55_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0493974.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            8877..>9015
                     /locus_tag="KPE55_002931"
     CDS             8877..>9015
                     /locus_tag="KPE55_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBG0493975.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HK"
BASE COUNT         3809 a          887 c         1438 g         2881 t
ORIGIN      
        1 attattatga aaatagtaac ctacaatatt cataaaggta tggattcaaa taatagatta
       61 actctaacta aaatggggtt atatttaaaa caattggatt gtgatgtaat ttgtttgcaa
      121 gaagtattat atcctcaatt tttagcttta aaagctgttt taaatatgga tggtgtcttt
      181 gcgacaaatg taaaaaaagt aaatatgata tatggtattt gtacatttac aacatctaaa
      241 atgttaaaca gtaatcattt tttcttaact agcaaaaaag aacaacgggg agctctttgt
      301 ataactatag atgcatatgg aagaattata aatgtaataa atactcactt gggacttgat
      361 aggcaggaga gagcaaaaca attagacgaa ataatagact ataggaacag attagtgggg
      421 atagttattt tatgtggtga ttttaatgag aaaaatgtct ttttaagtat gtttaatgat
      481 atggctattt cactaaacaa gtcttactta ccaacatttg aaaaatcaaa ctctagaata
      541 gattacattt ttgttaataa aaatactgaa ctaaaaggat acacagtaga aaaaatttac
      601 ctgtcagacc attatcctgt aataggttac atttaaattt caatataaat gaaattagtt
      661 gttataaaat aaacaatttt ttgttaagaa atagtaaaag aaatataata aaaaatatca
      721 aaaataaaac taattataag acataaattt acaataaagt tacatttgat taaattcctt
      781 gtttaacaaa agctattcaa atataatcta aggtagtttt gaaatgggaa ctctattatg
      841 caaatttaag ctagtgttaa aatgaggtga agttatgggc agaagaacaa caagagtttc
      901 aagattaggt aaagaaaaaa aacgaagtgt tttaattgta ttggcagctt tagttctaat
      961 aatgggtgga tttacttatt tttttaacag taaatttctg tacaatggga aaatcgctaa
     1021 aaatgtatat atagaaggag taaatgtatc tgatatgact aaagcagagg cattaaaagc
     1081 tataacagat aagtatactc cagaagattt aaatcttgct tatgatggga aaaaatatac
     1141 aatatcacca aaagatattg atttaaaata tgatacagaa gatgttgtaa aagatgctta
     1201 tgaaagtaca aaaaaagggt cctattttca aaacttaaaa aaatatattg atataagagt
     1261 gaataaggca aatatgaaga taaaagctga atatgatgag gcaaaactta gttccaaagt
     1321 gtcttctata gcagatagta taaatgttaa gatgaaaaat gcttctatta gtgttggaag
     1381 tggtggacta agttatacag actctgttgt aggtagagaa tttgatttgg cagcaaataa
     1441 agagtctata tataatatga taaaaaataa agagcataaa actttagaac ttaaagtaaa
     1501 tcttcaaaaa ccagatataa caactgaaca agtaaaaact gtaaattctg ttataggtca
     1561 atattcaact acttattcac aagctgtaga gggtaggtca tataatgtag gattatcagc
     1621 aagaaaaact agtgatgtat tattaatgcc aggtgaagaa ttttcataca ataaacttac
     1681 aggacctagt aataaagcaa atggttacaa agatgcacct gttatagttt atggtaagct
     1741 agaacaatca gcaggaggag gtgtttgtca aacatcttca acagtttata atgcggctct
     1801 tctttcgggt atggagataa ctcaagttac aaatcattca tctgcatcta cttatgtgcc
     1861 taaagggaga gatgctactg tgagcgatgg aggattaaac ttaaagttta aaaatccata
     1921 taagcatcct gtatatatta aaaattatgc aggtggagga agtgtatcat cagtaatcta
     1981 tggtaattct ggtgacaaac caaatatatc tatagaggta aaacaaactg gtcagaataa
     2041 atattctaca tatcgaatat ttaaagattc aagtggaaag gtcataaaaa aagaacatat
     2101 atcaaatagt tcttataaag aattaaaaaa atagataatt ttttagagaa atatttgtaa
     2161 tataaaaggg agtgtctcaa aatgaacttt ttagtttatg aggcactctt tttctgtgaa
     2221 atcaaatata ttttcatcat ttcaacaata caaaagaagc gtatctctat tttgagacag
     2281 ccttttttgt aaaaattaac actattagtt cttgttttaa tattacaatc attaatatat
     2341 agaaaatatt cctatatatt ggtataattg taaatagatt tagttatata aaatgtatga
     2401 ggaggtgaca tggttgaatt ttataggcaa tagatatgag gtagtaaatt ctgacgacgt
     2461 tttagaaatt aacaaaatct ataaagctag agatgttttt tacaggaaaa atgtgttaat
     2521 aaaagttata aagcacaata attatatctg tgaagatttt gtttcaaatt taattgatga
     2581 aagtactgct ttagatgaga taaactctcc ttatatacta aaaataatag atgttgggat
     2641 tcactgtaca gaagaaacaa cattatatta tgttgtaagt gaagacttta atggtatcgg
     2701 actagatgaa ttaattctag gtaattatct tcatttagaa gcaattgtaa acataatgat
     2761 acaagtttta aaagctttag aaatgataca taggaatcat tcatatcatg gtagtttaaa
     2821 atcgagtagt ataatagtag atacagaata caatataaaa atttgtgatt ttggaattac
     2881 aaaagcaaat aatggggtta atacaagaag ctatggaaac aggagatatt tatgcccaca
     2941 tcaactatgt ataaattata ctgataaaga aagtgatttt tttgcaacag gtcttattct
     3001 atttgaatct atatttaaaa aattgccttt tggagaatca aattcagaag aaaaaatgct
     3061 acaatctatt gataagggat tagattggaa tagtataaaa gcaattaatg gaaatactga
     3121 acttataaat gttataaaaa ggcttcttgg aagaaataat aagtatacca gagccaatga
     3181 tattattatt gatttaagta aagtaatgta tgaaaaagca tatatagaag ttgaagatga
     3241 gaaagaagaa gagcagaaac aagacaaagt tatacaagtt aaaaaagaat ataaaagtaa
     3301 aatgagaaaa aaattacata aaaaacttgt tgttgccggt ctagctatag tcatgatttc
     3361 aatgattatt attagttcca taatataaaa attgtatatt aagtatttta aaatcaatgt
     3421 ctataaagga tatacataga aattttagtg tagtcttaaa ttatgaaagg aagtggttat
     3481 tatgttaaca aaaaataatg aggtattaaa cgtctaggag tattttaaca aatagccacc
     3541 tgcttttaca gtttagaata aaaaagctgt ggctaaaggt cacagctttt tgctactttt
     3601 taaaggcagg agaatattaa aaaggagcaa attatgagca atataaaaat aatagcaagt
     3661 aataaatgtt ggattgaaga tatagcaagg aaacaacttg aagatatttc aaagttaaat
     3721 ggaattttaa gagtagtagg attacctgac ttacatgcag gtaaaatacc tgtaggttta
     3781 gcagttgaga ctaaaaatat tatttatcca catataattg gaaatgatat aggatgcgga
     3841 atgactttat ttaaaactgg agttctaaaa aagaaattta aaaaagacaa atggattaag
     3901 tcacttagta agattaaaga tttgtctgat atagaaataa aaaacacata taaagaagaa
     3961 tgtccaattt taaatttagg aactataggg agtggaaatc attttgtaga aattcaatgt
     4021 ataagtgaaa tttataataa agaacaattt gaacaattaa aattttcatc tgatgatatt
     4081 atgatgttag ttcactgtgg gtcaagaaat tatggagaag atattttaaa aaaattttat
     4141 gataaagatg gacttgaagt tgaaagtgag aaagctatag attatattaa aaaacatgat
     4201 aatgctttaa tgtgggcaga gcgtaatcgt gaagctatca gtaaaaaaat tatgcagtca
     4261 ataggtacat ctggagatgt tgaaacaata ttctctataa atcataactt tgtagagaaa
     4321 agggaagata aatttataca tagaaaaggt gctgtttcaa gtgaaagagg agctgtgatt
     4381 ataccaggtt caagaggcag tctatcatat atagttatgc ctactgaaaa tacagagata
     4441 tctttatatt ctctatcaca tggagcaggt aggaaatggt ctcgttcagt ttgtaaatct
     4501 cgccttaaaa gtaagtatag taaagatact ataaaacaaa ctaaatttaa aagtcagatt
     4561 atatgtaatg atgtaaattt attatttcaa gaagctccag aggcatataa gaatatagag
     4621 cagattattg aaagtttgat agaatatgaa cttattcaag tagtggcaac tatgaagcca
     4681 ttgattactt ataaaggata atttttaaag ttaaaaaaga ttaaaattat attataaata
     4741 gctaatttta tggaacattt aaaagtgttt tattattagc tattttttta ttttgtagag
     4801 acgataattt atagagctaa attctttgat atataatgat tacaaagtta gactgtattg
     4861 gaaactgcac atacatgaat tgatgacaat ataagtgatt ccaaaatata aatgaaagta
     4921 taagcttatt ttcactataa aaattttcac tgtacaaaaa cattaatgac attattttta
     4981 ttgacataaa tatataattt atggaataat attagaagca ctgatataag tgtttaaata
     5041 aggatagaaa aggagaagtg ttttatgaaa aaaaatacta acattaatga gtccggagtt
     5101 aaacttctta ataaataagg agtttgatat ataataactc cttatttatt aagttgttcg
     5161 caaaataact attatcaaag taaatgatag ggtgatttaa aagacgataa cttataaata
     5221 aaggagaaaa aataatgaat agtaatacat ttgaaagatt acaactaaat gaagttaaag
     5281 aattaataaa aatacattgt gtaagttcac taggaaaaaa tttaatagat aaattaactc
     5341 ctagtgggaa tattggagta gtaagaagga aattagcaga aaataaagaa gctagaaaga
     5401 taattgaaaa tagtaatcac ataccacttg aaggattgtt taatgcaggt tctacaatag
     5461 ataaaattga aaaaggaatg ataatagaac ctgttgaact tgtaaatatt gaagattttt
     5521 taagaggttg taggaagatg aaagctttta tgttagaaaa agaattttat tcacctacat
     5581 taagttctta tgccttaaat ataacagaat gtaaaagtat agaagatgag ataaattact
     5641 gtataaaatc taataaagtt gattctaatg ctagtaaaga attaaaaaag ataagacgaa
     5701 atatagaaat tacagaagga aagataaaag atagattaaa taaatttata acatcaactg
     5761 taaataaaaa gtatatacaa gagtttataa taagtaaaag aaatgataga tatgtaatac
     5821 caattaaatc ttcttataaa aatgaagtta atggtactat attagataca tcatcaaaag
     5881 gaaatactgt atttattgaa cctattagtg tttcaaattt aagtacagag ttaactatgc
     5941 taaaagctga tgaaactatt gaagaataca aaatattatc ttatttaaca gaacttatat
     6001 ttgagaagat aagtcagata aaattaaata tagagatact ttcagaatac gatatgttat
     6061 ttgcaaaagc taagtatagt caaaaaataa aaggaattac tccaaaaata aataataatg
     6121 gctatataaa aataattaaa ggtaaacatc cacttctaac aggagatgca gtacctttag
     6181 attttgaaat aggcaaaaat tatagaagtc ttataataac aggtcctaat gcaggaggaa
     6241 aaactgtaac ccttaagact gttggtttac taactttaat ggttcaatgt ggatttgata
     6301 tttctgcaaa agaaggtagt gaatttagtg tatttgaaaa agtttttgta gatatagggg
     6361 ataatcaaag tattgaaaat gccttaagta ctttttcatc acatataaaa aatattgcag
     6421 agattatgag tttatcaaat aattctactc ttgttctgtt tgatgaaata ggaagtggaa
     6481 ctgaacctaa tgaaggagct ggtttagcaa tatcattatt agaagagttt tacaaaatgg
     6541 gatgtataac tatagcatca actcattatg gagagataaa aaagtttgct acattgcatc
     6601 cagaatttga aaatgctggt atgatgtttg ataaagaaac gttagaacca ttgtataagt
     6661 taactattgg aaaatctgaa gatagtaatg ctctttttat ttctaaaaaa atgggaataa
     6721 aaaatagagt tttagataga gctaaagaat atgtaattga tagaaactac aatttagatt
     6781 tagttaaaag aagtaagcta caatctaata ttgaagataa aaatagttct gaagtcattt
     6841 taaactatac agattataat gtaggggata aggtaaaatt actagatgaa gatgattttg
     6901 gaattgtata taagcctatg gacaagttta ataatgtaga agtattgttt aatgataatt
     6961 ttgtttcagt aaatataaaa agattacaac ttagcataaa agcatcagaa ttgtatccag
     7021 aagggtatga tttagatgta ttatttacta gttttaaaga aagaaaatta gaaaaagata
     7081 ttcaaagagg ttcaaaaaaa gctcttaaaa agattcagaa agaaattaga gagaatagaa
     7141 aataaatata aaaagcacaa aagaaagcaa tctaaagaga aaagtttatc tttagattgc
     7201 tttcttatta acatatcttc ataaatccaa tgactaacaa gattaaacca cttaaccaag
     7261 ataagtttaa atttacgttt ttaacaaaca tatttcctag taaacaacca agtaatactg
     7321 caattatatt tgatattaaa gaaaatagaa taacttgtat ataattcaca cctacaaggc
     7381 cactaccaaa accgatagct aatccatcta aacttaaagc aagtgcaagt gtaaatgctt
     7441 cagcagattt aagcacttta gatttacttg tatctaagac atcaggttcg atacttacat
     7501 taaaatttaa tttaaggtct aacatattaa aattataatt ctttgagtgt atagattttt
     7561 tcttaaaata agatttaaaa aaactttcaa ttagtctagc acttcctaat aaaaataaaa
     7621 taaagaaaca tattaacatt gttatatttt gaggtaaaaa atctctaact attgtacctg
     7681 caaaaagcga tattgctaaa atagctgaac aaactacgct gataattata tttgaatata
     7741 agggaacttt aactttattt acaccatatc caaagcttgt cacaaaggca tctatacaaa
     7801 gagaagtaac taataaaatt gattcaatca cagtagatta acatcctttc tcttaaattt
     7861 tgtacttata ttagttatag tattgtaaaa atggataaat tgttacagat aatccaataa
     7921 attgttgata aaattccaac atcatacaac acctaaataa caaaagaatc atatcatata
     7981 ataatataaa accaatcata ataaagcggg tgaaaataat ggaacaaaat aataaggttg
     8041 ggaatttcgg tgatttgttg aagattttat tagatgaaaa agaattatca atgggagaac
     8101 taagtaaaaa aagtggaatt gataaatcta ctatatctcg tatagctaat aataaacaaa
     8161 agccaaatat aaatcattta gaaaagatgg caatacattt aaatattaac ctagaagaac
     8221 tgttgaaagc ttcaggatat gaatttagaa acagcaataa tcaaatattt aatgcagata
     8281 gtgattttag taactttgat gatattcttg gatttgcaaa tttaatcaat gataagaact
     8341 ttaatggaaa tattgaaaaa gagttaagta agtgtaagtt atatgtccaa acagatgaag
     8401 gtaaaaaatt actttttgat aattttaata aaaaaataga ttctattgaa aaacaaggtc
     8461 aatttacaga tagattaaga caaatgtatg ctgatttttg tacagatgga cttacaataa
     8521 agaaatattt attgattgga agtatgctac tttactttgt tatatcaaca gatgtgattc
     8581 cagacttcgt gttcccaata ggatttatgg atgatttagt agcattaaat atagttacaa
     8641 aattacttaa aaatgataag taagttttag aatcatattt aatatataaa taaatcaaag
     8701 tatataaaat ttattatttt ttatatgaaa caaattaaaa gcacaatgaa gtagattaaa
     8761 aatttaaaac taagaatcta atatatattt tgtaactatt taagtatata aaataaaata
     8821 atatccaaac taatacaaaa tagtaataat tgttttgtac tggaggatat atatttatga
     8881 attgtaaatg gatatctaaa atagatgaga gaaaaaaatg tcatagagaa gcagattctt
     8941 ctggatattg tatctttcat aaagaaaata agtctgatga agagatacag ttgatgatgg
     9001 atactcttca taaag
//
LOCUS       DAELNY010000155         8894 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.157, whole genome shotgun sequence.
ACCESSION   DAELNY010000155 DAELNY010000000
VERSION     DAELNY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.157"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(72..1844)
                     /locus_tag="KPE55_002932"
     CDS             complement(72..1844)
                     /locus_tag="KPE55_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG0493976.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1858..2787)
                     /locus_tag="KPE55_002933"
     CDS             complement(1858..2787)
                     /locus_tag="KPE55_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0493977.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            3015..3203
                     /locus_tag="KPE55_002934"
     CDS             3015..3203
                     /locus_tag="KPE55_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0493978.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            3196..3897
                     /locus_tag="KPE55_002935"
     CDS             3196..3897
                     /locus_tag="KPE55_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0493979.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAIIAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGQVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3899..4822
                     /locus_tag="KPE55_002936"
     CDS             3899..4822
                     /locus_tag="KPE55_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0493980.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4979..5902
                     /locus_tag="KPE55_002937"
     CDS             4979..5902
                     /locus_tag="KPE55_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493981.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKEREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5895..6632
                     /locus_tag="KPE55_002938"
     CDS             5895..6632
                     /locus_tag="KPE55_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493982.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            6643..7416
                     /locus_tag="KPE55_002939"
     CDS             6643..7416
                     /locus_tag="KPE55_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493983.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(7943..8551)
                     /locus_tag="KPE55_002940"
     CDS             complement(7943..8551)
                     /locus_tag="KPE55_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG0493984.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(8729..8845)
                     /gene="rrf"
                     /locus_tag="KPE55_002941"
     rRNA            complement(8729..8845)
                     /gene="rrf"
                     /locus_tag="KPE55_002941"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2932 a         1473 c         1274 g         3215 t
ORIGIN      
        1 atttctacat tttttataaa ttttattcat ttttatattt ttatgtatat aaattctttt
       61 ttgtaagttt attaatttct aattatcttc tttaaataca ttgaaactac atcagaaaat
      121 ctagttatat ctcttgaata tataaaatca tttccatata ttaatctttc agcttcaagt
      181 tctgattctt ttcctgtaaa tactcctaat actaatatat tttgctttct agcttttctc
      241 acttcattgg ctgtatctct caaaccaact atacccctat aagccttttc tcctcttaat
      301 gttctctcac tactttttcc tatctttaca tcatttggtc ttccatcgct gagaactatc
      361 aggattttat tttcttcatc tctatttatt agaccactac atatagattt tattgccaaa
      421 ccatctctat tattaccagc acaaaaatat tcaaatatat tttcactatt ttttatacta
      481 tcatcataat ctttaaatct ttttaatatt gtatagtcta aaaaactaga aaatcccatc
      541 actctatttg gtatgccaac ttctgttaga gcatttgcaa ttatgtaagc ttgaattgcc
      601 acatttccct gatttctact ttgagagcca cttgcatcca ataatatatc aataacgtat
      661 ttcccttttt cattttctat atctttataa aatattttat tattattact tctaccaact
      721 ctccaagctc tatttgctaa tatagtacca caatcagaat atactctact tttagcattt
      781 tcttctatta aaattcttga tagactttct tttaatctct ttatttgctg tctatacatt
      841 ctagcatgtt ctctaaattt tcctaaatta ttttcttttt gtcttgttac atattttagt
      901 tgaaatacat tattacactc acttctaaga actccatctg taaagtgtat tctacaacct
      961 tcatgaacat ttctacaatt ttgtgtctca atttttttaa tttcaccttt actcaaataa
     1021 gtttttccat aataatgttc tatttttgaa tatatcttat ttgcagtgtc ttcatcaaca
     1081 tatataactc ggtctgctga attgctacat ttagaatcat caaaatcccc tacaccttct
     1141 acaagcatgg atgaagatat tgaatctata tcactttcaa taatattttc ttcttcatca
     1201 ctataaagtt cttcatacat gaaatcagca aatgtatcaa aatttacttc tacactttct
     1261 acattatttt catcaataag actattttct tctccattta atatgtattc aaaataagtt
     1321 aaatatataa catctaatgc ttttaaaatc tccataatat cttgattggt atctatattt
     1381 cttatatcat taactattct tctagtcata ctatctaatg ctgggtgctt gtccatacta
     1441 taaagtacaa atgtgtattt aattttatcc ataatattat cattgtgtat tttggtaaaa
     1501 cgtgaaacaa tatctttata agcatttctt cttatgtctt ctatccctgg cctatcttta
     1561 atgacctttt cttcaactat tgtatttaat ataacttgaa ttagacccag tataatatct
     1621 ttgttgtatc cttttcttat tctatttgta agatattttt taactatatc ccaatctaca
     1681 tactttcttc tagcacctgc aattattgca aaatatagac ctatttcttt tgaagagtaa
     1741 tctttttctg atatatctat actttcattg taatcaccag ctattgtcca tgataagtta
     1801 ttagctctat tttcaaattc ataatcatca tatatccatt tcataatctc acctatatta
     1861 aatatgactt attggaaaca catctatact ctcccattta ttaggtattc tagttttaat
     1921 tacatctcca accatttctt tctcatatac atcaaaagtc tttcctgtaa gtcccatatt
     1981 aattgcaaga gttggtttta atcctcttct tatagtctta agagatgcca taagaccacg
     2041 taaatcaata gctttactag atatctctcc attttgagac ttcaattgta aatctaaaaa
     2101 tattcctgca aagtgtatta gtttttcttc atcagcatct gaaaattcat tttttaaaat
     2161 catcattaac ttatcttctt ctataggagg aatatctatt accataaatc tagataccag
     2221 tgcttcatta agttcttttg ttcctgcata ttcataattc atagtcccaa taaatcttgt
     2281 tgctggatgt agattgactc tctcataccc aggtacatca attattcttc tatagtctaa
     2341 agctgaatgt accacaacta tagcatcatt ttttgccata ttaatttcat caaacacccc
     2401 aaaacctcca ttaatagcac actcataaac tgagcctctt ctaagtttaa cttcattgtc
     2461 tataaatgta tctgtaccta taagtgttga actgtctgta tttatatgaa atgatgtatt
     2521 ccactgaggt ctattaaata tttcccctag attatcagca agtaagtttt ttcccgttgc
     2581 ctttggtccc gataataata tattttcttc ttctaaaata gctgctatac acatagacaa
     2641 gatatcttta ccataaaaat aagttttaga ctttgttact ctgtattcta aatctttttc
     2701 taatccataa taatttctaa aatacagtac atcttcaata agtttttcat ctaccccttg
     2761 cacccttaga ttatctatta tatccatatt tttacccctt ttatataaat agtatttgtt
     2821 tcatttctta tcaccatttt agcataaaag gatataagct aaaattgatt ttttaatctc
     2881 cccattttaa gtaaaaccat gcaccatttt atcactacaa aattaagagt actacttact
     2941 gatatttctt gattttgttc gtctatacgt atataatata tgctgataaa ttatgaggaa
     3001 aggatgattg aaatatggaa tatatgtctt gcccagaagt agcaaaaaaa tggggtattt
     3061 ctgaaagaag agtacaaaaa ctatgtgaag aaagtcgcat accaggagtt tcaaaactcg
     3121 ggtatatgtg gctgatacca aagaacgcag aaaagcctgt tgatggtaga acaaaaagaa
     3181 gaaaggaagt acaatatgaa tagcgttttg attatagatg atgacaaaga actttgtgct
     3241 cttatgaaaa aatgtgttga gcaagaaaat ttatctgcta ttattgcaca ttgtggtgca
     3301 gaaggtctac atttggtcga taaaaataga cacacactct ctcttgtaat tttggacata
     3361 atgatgccag atatagacgg ttttcaagtt ctgaaaaaaa ttagagaaac aagtaatatt
     3421 ccagtaatta tgcttactgc taaaaatgac gaggatgata aagtttccgg actacgttta
     3481 ggtgctgacg attacctaac aaaaccgttc agcataaacg aacttatggc tcgtgtcaat
     3541 tctcttattc gtcggtatac tatgttaaac cctgctttta taacagacac agattgcata
     3601 ataataaatg gcatgattat tgacaagaca aaccgaattg tttctgtcaa taatattcct
     3661 gttcaactta caagcaaaga atttgattta cttgcttttc tggcttctaa taagggacag
     3721 gtgtttacca aaaaacagat ttatacacaa gtatgggaag aagaatatgc ttttgacgat
     3781 agtaacatca tgtctttcat cagtaaattg agaaaaaaga ttgaaccaga ccccaaccat
     3841 ccattttata ttttgacagt tcgtggtgtc ggttatcgtt ttaataagga ggcttaacat
     3901 gaatacaagt gtctatctgc ttttgatatt ggttcttagc ctgattatta tagcgtacct
     3961 tgtttctgtc ctgctacgtg tccgaataca acttacactt ataaaagatg ctttagagga
     4021 tataaaaaat ggaaacctta atcgacgtat tttaacagat gaaaacgaca ttacaaaaca
     4081 aatttgctat gatattaacg aaatagcaat caatagccaa tttcaactta ttcagcaaaa
     4141 acaatcagaa caagcctaca aacagctaat gacaagtctc tcccatgatg taaaaactcc
     4201 acttgcttct ttggtcggtt atttggaagc agtagaaagt aaaattgttg taggagagga
     4261 aaaagacgag tatatccatg ttgcgtctaa taaggctcat tacttaaaga attttgtaga
     4321 aaatctattt gaatgggtaa aattggattc taaggaacaa gtttttcatt ttgatatttt
     4381 tgacttgaat gaattatccc gtaatattat atctgattgg atacctgttt tagaaagcag
     4441 tcattttgaa tatgaatttg atataccaga aatagagtac ttcttacgta tagacgcaaa
     4501 tgcatatacc cgtatcatta ataacctatt acaaaatatt attacccata gtagtggcaa
     4561 caaaatgaca ttgagaattt ttgaaaacaa agagcaagct caaattataa taacagataa
     4621 tgggaaaggt atatcttcag acaatctccc acatattttt gaaaggttgt accaatgcga
     4681 ccattcacga gcttcaaagg gaaatggttt aggcttggcg attgcaaagg aacttattaa
     4741 cgctcataag ggaaatatta ccgccaacag cactcccggt atgggaacag aatttacaat
     4801 attgcttcct aaagccctgt aaaagtacga ggctttctta ctctaaaaca agataaaagc
     4861 aagattatgg caaggttttg gcaaggttca tgttttataa ttctaattgt agttgatttt
     4921 gacgttgtat caactatctt ttgtagaaac aaatttatta aaagtgagga aattatatat
     4981 gaatgatttt gtgattgaaa caaagcaact tacaaaaatt tatgggaatc aaacagtagt
     5041 tgacaaagtc aatctccatg taaaaaaagg ccgaatatat ggtctgttag gacgcaacgg
     5101 agctgggaaa accacaatta tgaaaatgat tttaggattg acttcaatca cgtatggcga
     5161 ggtagatgta tttggagaga atattaagga acgtgaaaaa caagtatatc cccgtatcgg
     5221 tgcaattatc gaaacgccag gattttatcc taatctgaca ggaacggaga acttggagat
     5281 ttttgcaaaa ctgcgtggta cagccgcccc taattctgta aaaagagcat tggaaattgt
     5341 aggcttacct tataaaaata aaaagctgtt tagtaaatat tcacttggca tgaaacagcg
     5401 acttggtatt gccaacgcta tcctgcatga cccagaactg ttagttttag atgagccaac
     5461 caatggtctt gaccctattg gtattgcgga gatgagaaag tttattaggg acttgagcgt
     5521 tgagcatgga aagacaattc ttatttccag tcatattctt tcagaaatct cattgttggc
     5581 tgatgatatt ggaattatcg accacggtgt tcttttggaa gaaagtagta tgagtgaact
     5641 agaaaagaaa aacagtaaat atatcctgct acaagtttca gatattccga agacatcact
     5701 tattttagag cgtcaattta acacgaagga ttattccgtt caagatgact acacgttaca
     5761 cctttataac actaatctgg atatgggtga aatcaataaa gcacttgtaa tgcagaatgt
     5821 aactgttatt agttctcaac tttgtaatga tactttagag gactacttta aaaaaatcac
     5881 aggaggagag ggcattgctt aaacttattc aaacagaatt tcttaaactg cgtcgtagga
     5941 aacttatttg gctgatgttg ttgtctgctc tcattatgcc gtttttcgca ctactatact
     6001 tcaattattt tggtaaaaca ggtgttgagc caataaagtt ttataaatgg tctgcatttg
     6061 gttatacact ttggattatt ttgcctgttg ttttgggtat attttgcacc atgcttatac
     6121 acgaagaaaa ccagtatgat atgctcaaac agctttggat tgtacctatt agtaaaatgg
     6181 gatacttttt tagtaaattt ttcgttgttt tgatttactc tatttgtttt atgctgatta
     6241 cagccgttgc ttctgtgtta tttagtgtac tttctggata tgtcgtattt agctgggcaa
     6301 gcattttata tttgttgaaa aaatgtttag aaattggtgt aatcactgct tttgtaatgt
     6361 tgccgatttt ggcaatcgct acatctcaaa aaggatacat tctcccaatt tgtatcacat
     6421 tggtatatgc cttttccggt tttgttataa tgacaataaa tatgtattta cacccattat
     6481 ccagtatggg agttattatc atgagaaata aagacattcc aggtcttata ttttcacagg
     6541 aaattaatgt cccattagca tttctttgta tttgtatttg gagtattgta tcagtattgt
     6601 ttgcaaatat tgcactagga agaagaaagt gaggtgtaga taatgtggaa attactttgg
     6661 gctgaatgtc aaaaaattcg tcgttcaaaa attgtttgga ttacagtttt tgcatccatt
     6721 atgatagcag tcatagtttt tgcagggggg caggatgtat ataatgaccc agatttacat
     6781 tatggcttaa agagcgttta tgacggttca cggtatatag acaatgcagg ttggtatatg
     6841 gacgcagtac aaccttggtc aacttttttt gttcttcctg ctgtcattgc cctattagga
     6901 agctatatta tttgtcgtga agaagaggag gacactatta aatccctgcg tcttattccc
     6961 ataaatgaaa caaatttaac catagcaaaa atgattatta cttttatatt cagtatactt
     7021 ctttatttgt tactctttac tataacattt ttgactgaat ccgttttaca tttttccgat
     7081 ttatcgacaa aattggtttt aagttgcatg aaagagtatt ttcttgatgg cataggtgta
     7141 ttctttgcaa tttctcccat tattgccttg gtatcgcgca tgaaaaaagg atattggctt
     7201 gcattagtat tcacagaagt ttattctatt gctggactat ttgcgggtat gtcaaatgtc
     7261 ttacaaacct tttttcctat tgcggcaata tttaatattt ctggatacta tataactact
     7321 ggagaaaaaa ttatggggag tatcataagt ttattacttt gtgcctgcct ttctgcattt
     7381 atacttaaag ggctaaaaca caatgaaaaa aattgaaata ggtatatcaa aatgaaaaag
     7441 ataaaaaaac tgattattct aattgtattc ttgtttcttt ttggcatgtt acactgttat
     7501 actgaatgga aattcaacaa taacccacct aaaggggtaa tagaaagaat aaatgagtgg
     7561 actggattag taacttatgt taattatagt gaatgtaata ttagcaagtt attgtttcgt
     7621 attttaatac tataacttta aatatttggt tttaatcttc caaacgaata taaaaaaact
     7681 gtcacagagg caacatattt ttgaatattt ataaagtggt agttttgatt ttcaaagcta
     7741 ccgccttttc ttctatataa gcaaacttat cctcaacaaa gtgtaaaaca tggtacattt
     7801 taagaaaaaa gtaccacaga cacaagcaat aaaagaaacg ccgagaacct acaaaaacgt
     7861 agtgcttatg cagatttacg acgccttcta ttttctcaac cgtcttaatg accttgtaca
     7921 aaagaaattt ttctaatatt ttttataatc ttatcttgtg atgtttttta tgtataaatg
     7981 acttaataac aaatccaata aaaattatgg atattggtat aattagaacg aggaatttat
     8041 aatcattata atgtgacatt attcttaccc aactctcaga aaaaatatat cctatactga
     8101 ttaatgaagt attccatatg gttattccca cgatagatga tattacaaaa gttataatat
     8161 tttgccctgc tatacctgct ataaaagata tgtatgttct acataaaggg attaatctac
     8221 atacacaaac agaaatagaa gagtatttat taaaataatc ttgagatgca aatattcctt
     8281 tctttgcctt tggacatatc tctattattt tatttatgat agatttacct ccccaatttc
     8341 ccactgtata acaaaatatc gaaccaagta tacctgcaat tatacttaaa attaaaattg
     8401 tcagaaatcc aaaatgattt cttgcagcaa ttgctccaca taaaggtaaa acaacttcgc
     8461 taggtaatgg aaaacaagca tactcaatca taattaatac aaatacagag attaatccat
     8521 attgtaaaat aaaactattg attacagaca cttacttatc tatctccttt cttatttaaa
     8581 ggttttaagg gtacatgttc taaggattct caccctatat ttaaagaatc aatctcttta
     8641 aactattata tgtatttaca caaagcattt ataacatact ttaaaaatag taccttaaaa
     8701 taagaaactt aataaataaa aaaagattac gtggctacgt cctactctcc caggcagttt
     8761 cccaccaagt accatcagcg ctagagagct taacttctgt gttcggaatg ggaacaggtg
     8821 tatcctctct gctattgtaa ccacataatc tttatgctta atatttccat taagttttta
     8881 ataagtttat agaa
//
LOCUS       DAELNY010000156         8874 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.158, whole genome shotgun sequence.
ACCESSION   DAELNY010000156 DAELNY010000000
VERSION     DAELNY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.158"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(63..284)
                     /locus_tag="KPE55_002942"
     CDS             complement(63..284)
                     /locus_tag="KPE55_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493985.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(786..1766)
                     /locus_tag="KPE55_002943"
     CDS             complement(786..1766)
                     /locus_tag="KPE55_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493986.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1930..2802)
                     /locus_tag="KPE55_002944"
     CDS             complement(1930..2802)
                     /locus_tag="KPE55_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0493987.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(2966..3517)
                     /locus_tag="KPE55_002945"
     CDS             complement(2966..3517)
                     /locus_tag="KPE55_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0493988.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3537..4439)
                     /locus_tag="KPE55_002946"
     CDS             complement(3537..4439)
                     /locus_tag="KPE55_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0493989.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4454..6826)
                     /locus_tag="KPE55_002947"
     CDS             complement(4454..6826)
                     /locus_tag="KPE55_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG0493990.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(7047..7736)
                     /locus_tag="KPE55_002948"
     CDS             complement(7047..7736)
                     /locus_tag="KPE55_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0493991.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7977..>8874)
                     /locus_tag="KPE55_002949"
     CDS             complement(7977..>8874)
                     /locus_tag="KPE55_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0493992.1"
                     /translation="KLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGY
                     YFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMR
                     VKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKS
                     EVCLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKG
                     TITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFEN
                     SIDKFILNIYIPLDQDTDSWGD"
BASE COUNT         2863 a         1559 c          839 g         3613 t
ORIGIN      
        1 tatatgaatt ttatatgaga ttggtataaa tgatatatga aattacatat gtttatatta
       61 tattaaattt tattattaca ttttccacaa caatactcat atgtctccac attatttgga
      121 atatttacaa atacaaattc tttacagttc tcacaatact ttaatataga tatagttcca
      181 caagtgtaac attcaaatat tacacaatcc tcattttcct ctactatttc aaacttttta
      241 ctatgttcac cacactctgg gcacttgtga ttatttacat tcataaatac tcctccttat
      301 tagtatagtt acatgtcaaa aacttaatta atgtattcta tattacttat tatatattct
      361 ataataaatc atattctaat ataataaaac aaattaaata aaaaaaaaac cataaatttt
      421 aaatttatgg attaattctt gtaatgataa ctctatttga ggatgtgagg gagggttatc
      481 attacaatag ggtattatat tatggttttt aagctagtct ttctacacct catatgatac
      541 atatgctata tgaattttat atgattttta cataaatatt ttatgaaatt taaatttttt
      601 agtcattttg aaatttgtaa aaagttctct aaaaatccac agcatcataa acagccaaaa
      661 tttaagtaaa acatcataaa aaaatttgtc caataatttt aacaagtaat ctcacctaat
      721 tataattatt gacacaaact taaataaaca atctataaat cttctataaa tcttaaactt
      781 tccccctatt gatacacaat ttctagtttg tcaattttat tttgcatttc taagaaaatt
      841 tcttcaacag aattgttaaa tatattgcct aaatagattc ttttatcttt ttgaaatgaa
      901 taagatttta cactaccatc agaagaaata tataaatgac tccactctgc tccatatttt
      961 gaaaacctct cctttgtatc tttaaacctc atatcttcta tttttattgc tttttctctg
     1021 tctcctctta ctacagtgtg accccttttt attaaactgg ttatttttat atcttctttt
     1081 aatttaatac gtgtatcatc attttttgaa tatactttcc atatcacttt atgacgataa
     1141 ttatctaatt cctttattaa gcttctatca atagtaaaac tatctttagt taccacaatt
     1201 aaatcaaaat ggtctaacat atattgaaat atttctctca tacttttatt ttttaaaata
     1261 tcctcaccaa taacctctat aaatctacag cctttctcat acatgcagtt caataaactt
     1321 gtgatttcag aaaaataatc atttgtatta ttatctccat ctatttctat agtaaagtac
     1381 aaaggagtaa tgtatgattt atttcctgtt atctcaaata cacttgatag tgtctgtata
     1441 tcatcttcaa acgttaagta cacattctca taaaacataa aaaattccat aactttttct
     1501 ttcatattat ggatatttct atatctattg gataatatat ttattatatc gtcagtagta
     1561 tttttcccat cacattcagc cagtataaca atttgcttcc aacttaagct atccacattt
     1621 ccgatacttt ttttatatcc atcttcttcg tcataaatag gaccaattat aatgttttct
     1681 tttggccaca gatgaaccct agttccatct tttaatttcg gatatttatt ttttacagac
     1741 tcataaaatc tacttgtatc acccatgatt ctttactacc ccccatagtt taaatattct
     1801 atgctaaaaa tagcacttta tttttattaa ttcaacaatt attcaaaatt ggtcattata
     1861 tatgtttaaa tggatttaac atcaacctta tttatatgtt attgatgttt atatatgtta
     1921 tataaaatct tatttctcat acttaaatgt aatcttaact atctctggtt tacaattaag
     1981 cctaattaaa acttttgagc ctcctaagcc tttatttaaa atcatagtgc tattatcttt
     2041 ttcatatttt ccccagtcat acttaggaaa gaactctcta tttggagata atacacctcc
     2101 aacaaaaggt attcttatta tacctccatg aacatgtcct gctaaaacca aatctgctcc
     2161 ccattttgca tatttttcaa agaaaaatgg tgtatgtgct aaaagaatgt tatattcatt
     2221 ttcattaact ttacctagat tgtcttctac aaaattgtca cttagttcta attttttgtt
     2281 attagcaaat aaatatggat agcattccaa tggtataatt aaaccatata aattaagatg
     2341 gctattacct ctatatattc ttacagattc attttccata tttattattt tctttgaata
     2401 tagttcctta aaataatcct tatatagatg tttatacttt tttattaaag atttttgttc
     2461 atgatttcca attatatgat atacttcata tttttcaacc aaattatcta ttaaatctaa
     2521 tgcaacttga aaatttttat tttctccatc tactaaatct cctgttataa atataacatc
     2581 tgggtttaat gattcaattt tatctatcaa taccttatta tttttatcaa acaccttatt
     2641 atgtaaatca ctcacttgaa ctattttaaa gttatcaaat tcctttggaa tttttttgtt
     2701 gaaaatagta tattctgtcg tttctatatg ctgagtttca tacttataca gaatatacaa
     2761 taatattaat actaatactg tcattgaaac ctttataatc atattctctt cctctttatt
     2821 ctattttgtt aatattatta taatgctttt atgttttttt taaaacaata acatcatcac
     2881 atacatataa atcttttcga cattataaaa ataagttatc cttttctaaa attttcattt
     2941 attttagaaa aggataacta taaaattatc tataaacctc tatattgtga aatatagttt
     3001 ttcctttatt taaattttta tatatatgct ctaaagttcc atcaaaccta atagaatgtc
     3061 cagactctaa aatataagtt ttcccatcca cattaaggtc aagtttacct tctattacga
     3121 gtacatactc ttctaaaaca tgactgtgtg tagatgaaac atgagtacag ttttcctcaa
     3181 gctcaattat aaaaagttca aaccttcttt gagggctaaa agggtatatt ggatataact
     3241 tcatgttccc tttttgctca acaataggct ctacttcttc tttctttata acctcatagg
     3301 tatttgtatt ttcatttaat agtgtagtaa aatttatttt aaatccagat gaaattttcc
     3361 ataaagtaga tactgtcgga cttgattcac ctctctcaat ctgtccaagc attgctttac
     3421 tgacacctgt catctttgaa gctttgtcta aacttatgcc catttcttgt ctcaataacc
     3481 ttaaattttc tcccacatta ggaaatttat tatccatgtt aaaatcctcc tgatagctat
     3541 ttgtattcta tgatttttac tcctgtaagt tctactatct cttttaattt taacatatga
     3601 ttgttacaaa tttcatcaaa ttcatatata taatcttcaa gccctaatgc cttatattta
     3661 tcgattccaa atttatgata tggaagtaac tccatcttgc ctcctggaac attttgtttg
     3721 acaaataatg cagtatttct tatattttct tcactatcat ttactccata aataattggc
     3781 actcttataa ccatagattt attcaacttt gaaagcctac atatattatc taatataatt
     3841 ttattactta ctcctgtata ttctttatga atattatcat ccatagattt tatatctaca
     3901 aatatgtggt ctattttctc aaatacatca tttaccttat tccaatcaaa atatccacaa
     3961 gtttcaattg caatatttat gcctttatca taaaaaatat caactaattc tcttaaaaaa
     4021 tcaatctgaa gtgtaggttc acctccagaa aaagttatcc ctccattaga ttcaaagaaa
     4081 aatataaaat ctttttcaac ttcctctact atttcttcaa cactcatttc ttcggtcatt
     4141 atacaaatgg catctttaag acataccttg atacactctc cacataaatc acatttatta
     4201 tttatttgcg ctctatcaaa tagtgaactt atattttgag gacaaacatc tatacatcta
     4261 ttacaggata cacatttatc tttcataaca cctaatttta ctatgttact ccaggaatct
     4321 ggattagaac accacttaca ttttaattta catccttcta aaaatatagt agtccttatt
     4381 ccatctccat catttactga aaaatgttgt acttttacaa ctctaccttt tttactcata
     4441 atctacctca taattaaaaa ttgctatgtg aatttctagc tattatagca tcttgcattt
     4501 ctcttgataa attaacaaat tgagtactat atccagcaac tctcactaat aaatctttat
     4561 attcttctgg attttcttga gcttttaata gagtatcatt tgatatcgta ttaaattgta
     4621 catggaatcc tcctaaagca aaatatgatt taatcataga tgttagattt ctaagtcctc
     4681 tttcagtttc caccaaatct tgattcagtc ttaaatttag aagtgttcca ccagtatgaa
     4741 catcatgatt tatttttgat gcagatttca ttgctgctag tggacttgtt gtatcagtac
     4801 caacaaatgg agaaactccc tctgtcaaag gttttgtcgc ttttcttcca caaggagttg
     4861 cgcctacgat ttttccagtt ggtacatagt ttgatatacc cataaatgca ctattaaatt
     4921 tagagccaaa aatatccttg tattttctag tttcagtata ataaaaatca gaaacttcaa
     4981 ttgctaatga atctacataa tcattatcat ttccatactt aggaacatcc aacgcaagtt
     5041 ttcttaattc ttcataacct tcccaatcat tatttagtgc ttttgttaat tcttctaacg
     5101 tagtcacctt atcctcaaat actaattttt ttatagctgc caatgaattt gaaactacac
     5161 ctaaccctat accagttaat acaggaccta tattatattt tgctcctcct ttacttaaat
     5221 caactccctt atccaaacat ccatctacac aagtagataa aaatggtctt ggtaccattt
     5281 ctttatgaat ttgttgagca acagtagttc ctataacaga actttttaca agatatgcta
     5341 attgctttaa aaactcatcc ttaagttctt caaaagaagt aaattctgtg atattttttg
     5401 tatctaaccc cattttttct ccagttaatc tactcttacc ttcattcata gcatattcta
     5461 aagctgctgc aaaattgata tttacggctg aagtccactg tcctgttttt ctaaaatgag
     5521 gaactacaca accacagtta ctccaatctc tagcatcttc tgcatcatat ccatcttgta
     5581 ataacatttg tgcccctgca ctatcgctgt gtatagcagg gaaaccagtt ccttgtttta
     5641 ccaatttagc aactgccatt acgaaattgt ctggtgctcc ttgactcact ctaacactta
     5701 atcctggttg gtgagttttt acactttctg tagcctttaa acacatatat gagatatcat
     5761 tcgttccatc agtaccattt ctctttttac caccaacagt aagattttgg aattgattat
     5821 atcctgcaaa gtaatttgct gtatttgctg atatagtcca tacccattct gataacttaa
     5881 tccaaagtgc ttctatcaat tcttgagctt ctgattcagt tattttacct tctctagcat
     5941 cattttcata atatggatac atatattgat caaatctacc taaatttaat gccaatggat
     6001 tttctgatag aataccacct aattgaacaa accatacaaa ttgtattgct tcataaaaat
     6061 tagttggagg gtttgctggt acattcatac atatctctga aatttttata agttctaact
     6121 ttctcttttc atctgtttct tgcttactca tctcttttgc aagatttgaa tatctttgtg
     6181 ccaatgtcat aataccctgt gcaactatag aaacagattt gtaaaaatct atcttttcaa
     6241 tattttctga aactgatata tctaattctt taattttttt atctgcatct tccttaatcc
     6301 ctttatatcc ttttacaaac aatatatctt gataatctgg tgttacttca ccaactgcct
     6361 gtctccactt agaatcatta tctattattc ctgtatctac taatatctta gctgtatctt
     6421 ctgggattct tgctaagaaa acttcttcca aagattttcc cttccaatat ggaaatattt
     6481 cttttcttat aaattctatt tgttcttttg atattacata tgggtcttga gttcttttat
     6541 caaaagtatc catttctttg tctacccatt gccaagaaaa ctctggtgtc aatatccctg
     6601 ttcttctaaa ttttcctgct gtacctacta ttaattcatt ttcaaagatt tttataggaa
     6661 gttctttaca tgtctccaaa aaagctttag ctcttcttat acagatagct tctccctcag
     6721 tttttttatg tgattctgta aaaattctag ccctttcaca acatatcata ggtttagaat
     6781 taatatattc ttgtctaaat gcttctattc tattagtttg actcatttaa aatccccctt
     6841 tggataatat atactacatt tttgtatgtt ataacatact ttaattatat tatactgtcc
     6901 tttagcataa aatggaagca aaataactta ttttttttca tctaccttga acacctatat
     6961 attaatactt tttttgttaa atttataaaa tctaaaaaaa ttttctatga tatattttct
     7021 atattgttat agttatgtct aatatactaa tgatttgaaa tatcaaattt gtatactaga
     7081 cttagtttta aaaccttagt tgattgttta actttgttaa cttcaacatg tatatccttc
     7141 atataaggaa gctctacaac acctactccc ttattaaagt cttcttcaac catagaaaaa
     7201 atttctttct cagttaagtt cgtatcagtc aatgatttaa ataaattgta tataaattta
     7261 acatatttat cactctcaga taacttatca tcttctggat actctttagc acagagaata
     7321 tctattgtta cttttccttc tttatcgtct tcagacatag aatagaataa acttcgaata
     7381 tttttttcag gtggtttttc agcaccattt atagatacta caaagctacc atctgatttt
     7441 gagttagtaa tactataacc actgtctatt atagctttcg aaaatctttg agataaatct
     7501 ttttttactt cttctctacc tttcgcatca ttaatctttt gtgagtaatt attaaatagc
     7561 tcaatagatt catctttttt atcttcaact tgttctttca atgaatcttt actaatttgt
     7621 tctgagctac ttaagttctt atcagataca ggagacatac accctaccat caatgctaag
     7681 ctaagattta aagccacaat tattgccact ctatttatat tcctttttac tttcatacac
     7741 aatcctccca tcacactaac cgctctaaag tattattcat tgcactagaa tatatttata
     7801 ttttctttat attaaagatt tattattcaa tacatatcac cttcctatcc aattcttata
     7861 acttactatt agtaaaatta taagctcctt aatacagaaa taaggatgtt atcttttgtt
     7921 attaaaagat aacatcctta tttttttgta tcaatacata tgtcccaact ggtaacttaa
     7981 tcgccccaac tgtcagtatc ttggtccaat ggtatatata tatttagtat aaatttatcg
     8041 atactatttt caaataataa ttctccatta tatttttgta aagaagattt aatactctga
     8101 atacctattc catgtacgaa cttatccatt ttgtttgtta acaatatatt tttattaaaa
     8161 gtaagctgat ttagtttact attttcacat ttaagtacaa aaaatgacct tgttatagtt
     8221 cctttaattc taatatattt attaatattt tcatccacta ttttatcaca tgcttctatt
     8281 gcattatcca aaatattaga aaatatgctt gacacatcta taggttttac aaaatttact
     8341 tttgaaaaat ttatagagca agtaaattta attccttttt ttaaacatac ttcactcttt
     8401 tcgttgatta taatgtctaa tatcatattt cctgtattat agatatattt aaatgttttt
     8461 atttcatctt ttaaattgtt aacatattca ttaatttctt tgctattgtt tctaaggttg
     8521 tctatacaat aaatgtgatt atttatatcg tgatatagtt tcttaactcg catatgagat
     8581 tcttgaatac ttaaataatg cgcatattgc atatctaact tattttttat taattcagct
     8641 tgagatttta atctattctc ttttattatt tctttaaaca atagtataag aaatatacta
     8701 ctagctccta ccaattttgg attaacaaaa ttatcaaaat agtatccttc tgaatgtaat
     8761 gacccaaacc gttctaattt ttttaataca taatttacaa gcatattcat tgtattaact
     8821 aataaggcaa ataataaata tacataattt ctcttatctt ggctaattaa tttt
//
LOCUS       DAELNY010000157         8873 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.159, whole genome shotgun sequence.
ACCESSION   DAELNY010000157 DAELNY010000000
VERSION     DAELNY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.159"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..241)
                     /locus_tag="KPE55_002950"
     CDS             complement(<1..241)
                     /locus_tag="KPE55_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0493993.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVITILSVFLFFFIWYATNI"
     gene            complement(242..994)
                     /locus_tag="KPE55_002951"
     CDS             complement(242..994)
                     /locus_tag="KPE55_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0493994.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(1310..2464)
                     /locus_tag="KPE55_002952"
     CDS             complement(1310..2464)
                     /locus_tag="KPE55_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0493995.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSFLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(2467..2934)
                     /locus_tag="KPE55_002953"
     CDS             complement(2467..2934)
                     /locus_tag="KPE55_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0493996.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(2948..3436)
                     /locus_tag="KPE55_002954"
     CDS             complement(2948..3436)
                     /locus_tag="KPE55_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0493997.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(3450..4781)
                     /locus_tag="KPE55_002955"
     CDS             complement(3450..4781)
                     /locus_tag="KPE55_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0493998.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(4795..6360)
                     /locus_tag="KPE55_002956"
     CDS             complement(4795..6360)
                     /locus_tag="KPE55_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0493999.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSPATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQAYE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(6530..7315)
                     /locus_tag="KPE55_002957"
     CDS             complement(6530..7315)
                     /locus_tag="KPE55_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0494000.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(7482..8681)
                     /locus_tag="KPE55_002958"
     CDS             complement(7482..8681)
                     /locus_tag="KPE55_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG0494001.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         3097 a         1503 c          904 g         3369 t
ORIGIN      
        1 atatatttgt cgcataccat ataaagaaaa ataaaaatac tgatagtatc gttattacag
       61 ctactaacac tgatagtgta aattttttat tttcatcttg taacacattt atagcatcac
      121 catatcttaa taattcaaaa ttagttagca caactactga aaaaaccata gatataaaaa
      181 atgctagata agttcttatg ttactcttaa aattattata agcaattcca agtatactca
      241 cttacatctc acctcccata gaggctagca tatccataat ttttccataa aattcttttc
      301 tactatttcc tttgtttaat ttacacttta tatttccatc atttatcata tatacttcat
      361 ttgaataact tgctgttaga gggtcatgtg taatcattat aatagtagcg ttattttctc
      421 tgtttatttt ttctaatgat tctaaaagtt catatgctga tttagagtct aaagcaccag
      481 ttggctcatc agcaaatatg actgttggat ttgtaattaa tgacctagct gctgcgcctc
      541 tttgtttttg tcctcctgat aactgatatg gatatttatc taaatgattt tctaatccaa
      601 attttttagc tatagaaaat accttagctt ctatctcctt acttttcttt tttcctaatg
      661 ctagaggaag tgctatattg tccattagtt tcatattgtc taatagatta tagtcttgaa
      721 atataaaccc tattttttct cttcttaatt ttgatagttc attatttttt acttttgata
      781 tattaattcc atctagtatt atttctcctg aagtttgttt gtcaagtgta gacattaaat
      841 ttagcaatgt tgtctttcct gcaccagatg gacccattat tgatataaaa tcaccttcat
      901 tgactgtaag agatatattt tttaacacat ttgttttaaa tccttttatt ccataatctt
      961 ttgatatatt ttttattttt aatatttctt tcataataat agcctccagt tgctaatttt
     1021 ttaatatata tttattttaa aggttttata taaataaatc cttaagtaaa tattacattt
     1081 taactgttag cattacactt ttgtaacata tggaagacta ttataattat agtagaaata
     1141 tcttaaaata aaaacttgct tcactttttt gtataaacaa agaaaagaag ctcctaagaa
     1201 gcctctaatc tatacaataa ttcttctcta taaaaaacca aaatacacat tgattgtggc
     1261 aaatacagat actataaaca agtgatatta ctactatata ttaaaacaat tattcctgaa
     1321 gataattcaa taagactatc aagcaataag ctaagcatcc aaaaagactt aataccatta
     1381 atgtataatt atacccaata ttaatgctca aaaaaccagc aacagttgga ataatcagaa
     1441 atgaacctcc cataaatata cttaaagcta ttaattggct actacttcta agttttttag
     1501 gagaaacttc aaatattaat ctttttgaag taattaaaat taaaggagta gaaaacactt
     1561 gaaaaacaca cagtataata atgatatttg aatcatttgt taaagcaaat aatagaaact
     1621 gaactgttat cataatagca cttaatttta ataagtttaa actgctatat ttttttaata
     1681 aataagaacc agccagatat gttggtatct ctaacaatga ctgtaaagac cactttaaag
     1741 atatctgaga atttgtagca tttaaagcta tcattttatc tattacagta cagttatttg
     1801 ctaccaccat tgagtataat aagaataaaa tacatactag taagatatat tttttatctt
     1861 ttaataattc aataatatct ttattagtta catttatttt cttattaatc ttttcaatat
     1921 caggtaataa tagtatattt ataataagta atactacaat taaaccaatc gctacagata
     1981 atccaaaata tgagaatcta agaatcaagt aagatgctac catacttcca atagcccatc
     2041 caatcgaccc aaaagtctta ataaatgaca aataattatt aatattatct ccacaaccta
     2101 ttatccatgt atcatataaa ctacagcaag tatttaataa tcctcctgat aaagcaacaa
     2161 gtaataagta tattataaaa gttgcagatt cagtagaaaa cataataact gatgcaattc
     2221 cataaactga taaactaatt aaaacaattt ttttcattgt ttgatactta tcagctaata
     2281 ctccaaataa taattgaaac aatatattgg ttattgcata gctcgataat ataattcctc
     2341 tttcaaacgc tgagtaacct aattttgata agaaaggtgt gaattttgtt gaagcaaagg
     2401 caaaaataat atatgagaaa aaatagaata tgataaattt aaatttaatc gctttattaa
     2461 tcataatcaa tcaaaattga taactttaat accactattt ccttcctgat ttaagtttga
     2521 aagatgatat tttaccgtat taggattctc tacaataact aaaggaatat ataaagtatt
     2581 ttcaagtaac ttattatcaa tttctactaa cttttccccc atattaactt cttgaccttg
     2641 tttaacttct aatttaaagt tttctttttt tgttgttgca ctatccaatc caatatgaat
     2701 aagaatttga acatcatttt gcaatttaat tcctatagca tggttagttt gagcaataaa
     2761 acttattact cctttagtcg gtgatttaag aatattgtcc tttgctatca tagcaaatcc
     2821 atctcccatt accttagttg aaaatacttt atcaggaatt tctccaagat taattatttt
     2881 tccatcaatg tatgaattaa ttgtaattgc ttcttttttt cttttaaaga acattataga
     2941 cactcctcta cataattaac tttattctaa ttatcattac aaataacgat attaagcata
     3001 ctgcaattga ggaaaaaaga gccttatagc tctttcccca ttgtagttct ttaatactgc
     3061 ttatgatttg tttaattcca acaaatttat aaatataggt aaacatgata acaatagtta
     3121 ttgtgtaaat ttggaatatc tgccctaata agactaaata gaaagcaatc atgttttctt
     3181 ttatctcttt attctttttt atattgtaaa taacatatgc actgtatact gcactaaagt
     3241 tagagattaa atttaaactt gcattaacat cattgtttaa aagttgtgaa aatgaaatat
     3301 ttgtaaataa tgatgtcaat tctaaataca ctatatataa aacaggtaat attagaaaac
     3361 aaaccataac cactgaaaga aatatatttt catcttttaa tactgctgtg atatttttta
     3421 ttttatttgt tatcatgttt ttgttctcct tatttaaaag ttggccagaa atccttatta
     3481 gccacaatca tttcatctaa gatttctttt gctacttttg cacttggaac agttttactc
     3541 atagtaaacg actgccataa tttttgataa cttttttcta tccatgcttc tacagctaat
     3601 ttttcagaag caacttgttg ttccattaaa cctttttgga aagttggaat tttacctata
     3661 gacataggtt caaccatatc ttttccaaca aggcaaggaa tttcaaccat tgcatcatct
     3721 gcaaaatttt caatagctcc attattagga acaatcagta aaaatctttc ctgagtatta
     3781 aatgctaatg cacaagctaa ttcaacaata aattgagaat gctcacttgc atcaaaccat
     3841 gtatcccttg ctgtgccatt ttttataatt ctttcacatt cttcgaatgt atccttttct
     3901 cttctatcca taatttcatt tgctcttgta taatttggat tagcatgttc aacagtttca
     3961 tcagccatta aataatattt taaataagta ttaggtaatg tagtcggttc tatttccaat
     4021 aaatcctttg ctgctctcat agtttctaac caactagatt ctttgtgttg accttgaggt
     4081 gtaaattcct catatccttt tttagcacaa tattcttgta atttaggcat taaatcattt
     4141 ccttctttgt ccttaatact tgtccaccac ccgaaatgat tcaatccata atatctaaca
     4201 tcaaagtctt tacgagaatt taaacctaaa actctagcaa atatttcctc taaacatact
     4261 ggcatatcac aaatattaat aattcttgaa tttggtctta atacacgaca tgcttctgca
     4321 actatagctg ctggatttga ataattaagc atccaagcat ttggagaata tttttccata
     4381 taatcaatca tttcgattat tggacctatt gttcttaatc cataggcaat tccacctgcc
     4441 ccacaagttt cttgaccaac aaccccatat tttagtggaa ttttttcatc ttgttcacgt
     4501 aaagcatata aaccaacacg aatttgtgcc attacaaaat caatatcagt aaatgcttct
     4561 tctggatttg ttgtataact aaatttaatt tctggatttt tctctctaac aattattgca
     4621 catgcttcag caacttttct ttgtctctca tcattattat catataattt aattgattca
     4681 ataggaaatt tagcaacact ttcaattaac attgttacaa ttccagcagt ataagtacta
     4741 cccccaccag caattagtat cctctgtctt tttctttcca tctttccttc tcctttataa
     4801 taaattttca aattcttctc ttacacttgg tactgaaagc ccaataataa tctgaatagc
     4861 ttttccatta tgcactaaac catgtgcacc tactgcctta aaatcttctt ctttagaaat
     4921 aattgagtca tctttcaagg ttaatctcaa tctcgtagca cagtttgtaa catctacaac
     4981 attttctgct ccaccaacta attttatgta ttcatatgct tgttgagagg ctttactcaa
     5041 tttatttcct ttttcatctt tcgtttctaa atattcattt ttagtatata gttttgtttc
     5101 ttcactatct tcttctctac caggagtttt catatcaaac ttcttaatta agaaagtaaa
     5161 tacaaagaac cagataaatg aaaatataat ccctacaaca acttgaacaa catatacctt
     5221 ccagtgatac attccaagag gaagccaatt taatacaacc cagttaataa ttcctccacc
     5281 aaaatcacca acaactccta aggcaaacga aatcgtttgc aagcatgcat ctaaaaatgc
     5341 atgtactagg aataaaagtg gtgctacaaa taaaaatgta aattctaacg gctcagtaat
     5401 tccagttaat actgcagtaa gagtagcagg aatcattaaa cctaatactt ttttcttttt
     5461 ttctggcttt gcagttacat agaaagcacc aaatacacct aatgaaccaa acaccttcga
     5521 taatccggat aatgaaaatc cgattggata catttccttt agagtcttag ctgaagtttg
     5581 aaaatcctga atatgagtag cccaatatac tgatgtacca cctggaacaa caacactatc
     5641 ataacaaatt ggtgcatata caaaatgatg taatccagtt ggtattaaaa tcttttgtaa
     5701 gaatgcataa caccaaactc ctatatttcc actatttata aaaaacgttt gcaaatgtct
     5761 aataacattt tgaatatgag gccaaataaa acaagttaat aatgcaactg gtatcataac
     5821 aaagaaacaa attgcaacta caagtgatga gcctctaaat aatccaataa aatctggcag
     5881 ttctttatca taaaatctat tatgaatata aatgacaata cctgatatta acaaagcacc
     5941 aaccatacca gtatctaatg ttttaatacc agcaatgctt acaagtccac ttccagcact
     6001 tatttcagct gcgaaatcaa ctccaaagct ttttccccaa aaacttaaca tagaacctac
     6061 aaaataatta aaagtcaaat acgttgctaa agcctccata caagctcttg cttgttgctt
     6121 ttttgcaagt gctattggta aagaaatagc aaataataat ggcaattgat taaataccat
     6181 ccaagctcca ctagatatta caccccaaaa ttggtaccaa atattttctg gtgtagcagg
     6241 tgaacccata attgactgat tttgaaatac aattgagaat ccaaccatta tcccagcaaa
     6301 agcaaataat agtgttggtg taaacattgc ccctccaaaa cgctgtattt tcttcatcat
     6361 ataaattact cccctcctca tatttttagt ttatctttgg ccttagatat ctattatttt
     6421 aacaggttaa taaatatata caatctttag ggtacattcg gagcttattc acagtcaaat
     6481 aagtttttgc acatttttat gttcaaattc tcataaaaaa aaagaatgac tacaatttgc
     6541 aatcaatccc ttctgtaatc attctatgcc ttttataaat actatattta ataaataata
     6601 actcaatcaa tataaaatat aacattgtca atttataact agggtgcatt tgtaaaaatg
     6661 agatattagt cgctgaaata taaattgatt catcacttaa atcagtcaat gtattatttt
     6721 taaactctgt aattgaaatt attttagttc ctattaattt taatcttcta gcaatattaa
     6781 gtgtattctc attctcacca cttagggata taataatcat aacatcattt ttactgacaa
     6841 tattgtaaaa agaatcatca aatgttcttc cctcaaaatc atatattatt tcttgacatg
     6901 ataagaacat tctctttatc tgttttgcaa ccacattttg aatatcacct gaaccataag
     6961 cgtatattgt tcttgattca taaagaagtt tacacaatgc atcataatct ttattttttt
     7021 gatcttcaac atacttaatt attgaattac atgcaatatc aaatgactga ttttctatga
     7081 catgaacctt ttctgattcc cagcttaata atactttaaa ctcactgaaa ccttttaaat
     7141 ctaatttttt aacaaatctt gtaatagttg cacgagacac attacatttc atagcaagtt
     7201 catttattga catcttacta caatgttttt tatttgcagt tatatatttc catattaata
     7261 aatctgtatc attcaattta ttataattta aaaaaatcat ttcttctaat ctcataaact
     7321 attaccccct ttaattaaaa atcgtcataa atattatacc aatattcaac attaaattac
     7381 agcgtattct tatatttact atttagccca taaaattgac tataaaaaat actgctataa
     7441 ttgatgttta aaaaaatgta gttttaattt tattctttta tctaataatt aatattttca
     7501 agttctagtg cttttcttat acaattatca tctttaaaat cagcagtcct tttacaatct
     7561 tttactttaa attctggaat agttttaaat tcctcatcac atattccatt cttatttaaa
     7621 tacatacaac ttgccattct aactattaga ccactatttt ttaaattaaa caatacaggg
     7681 tctatacacc ctccatctcc gccagtagtt tcaccaacta aagtcgcaaa tttattttct
     7741 ttacaaaaca ttgcaaatga ctctgatgat gaatatattt ttctatcaac aagtaagtat
     7801 atattaccct taaattttga atcatctgta gattttatag tataggaagt atcttcaaaa
     7861 tcattaaaat cttttataat ttctttaggt gcattttcct ttacacttgc tggtaaattt
     7921 tctacaggat ttaattttat atttcttgca tttgtatatt tttttactat ttcattatta
     7981 tttctataaa ttctataacc atttattttg acatcatttt ttatgagttt agatacgatt
     8041 ccctgccaat acctatcact tccacctaaa ttacctctta tatcaataat taaagcttta
     8101 tgtttttcta aagtgttaat ataatctcca attattttta aatcattatt tatagagcca
     8161 ttctttgatg acattgatgg taaatacata tatccaatct gacccttaat aacatccttt
     8221 aaaattaatt ctttttttac aaaaatatca tctggcattt ttattttaag attcatagag
     8281 tcatatctat ccacaacttt tttatcatta agaaaatcat accaatcatt attagagtat
     8341 gatttcttaa aaagttcata tcttttctta ttatctatta cttctgtgtg tttattattt
     8401 aaatcagata atatagatga cagttcttct ataaattctt catcattttc agtgtttttt
     8461 attcgttttg tatattcatc cttatttgct aaccaatcaa tattattaag cctcttattt
     8521 acacctagat atggatatcc tgtctttaca acattataca cataattaaa gtcatccacc
     8581 ttttcctctt cagacaactg attcttttga gaataagtag aattacctaa taatattata
     8641 aatgctaaaa taattactaa aaataatact ttctttttca tgtaattctc atccactttc
     8701 agattttata ttttaataca ttgtatcatt attttattat cacctaaaca gttaaaagaa
     8761 attcttgaag atatattcca ctattattct atttaaaaat ttttatttgt attttcaaaa
     8821 ttatttcatg aaagttataa aataacaatt atattcaaat atctatttta ctg
//
LOCUS       DAELNY010000158         8794 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.160, whole genome shotgun sequence.
ACCESSION   DAELNY010000158 DAELNY010000000
VERSION     DAELNY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.160"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            174..899
                     /locus_tag="KPE55_002959"
     CDS             174..899
                     /locus_tag="KPE55_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0494002.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(1027..2223)
                     /locus_tag="KPE55_002960"
     CDS             complement(1027..2223)
                     /locus_tag="KPE55_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494003.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDNKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(2226..3251)
                     /locus_tag="KPE55_002961"
     CDS             complement(2226..3251)
                     /locus_tag="KPE55_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0494004.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(3323..4453)
                     /locus_tag="KPE55_002962"
     CDS             complement(3323..4453)
                     /locus_tag="KPE55_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0494005.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            complement(5047..5442)
                     /gene="tnpA"
                     /locus_tag="KPE55_002963"
     CDS             complement(5047..5442)
                     /gene="tnpA"
                     /locus_tag="KPE55_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0494006.1"
                     /translation="MKLDSNNHSVFLLHYHLVMCIKYRRKVIDDSISDRLKDIFVDIA
                     PNYGVVLIEWNHYVDHVHLLFKAKPNTEISKLLNAYKSASSRRIKNEFPSIRKYLWKE
                     YFWSKSYCLVTTGGATLEDLEQYIENQEE"
     gene            complement(5607..6614)
                     /locus_tag="KPE55_002964"
     CDS             complement(5607..6614)
                     /locus_tag="KPE55_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0494007.1"
                     /translation="MRLREYIGDKVVFLGTNLIIFLIASGIMLYSKINVVLIFMLFCI
                     WFLPLFTYMLIDYLQYKKYFNSIDNILDALDKKYLLPEVIDDVNFVTGEKINDILKVI
                     SRNMHENIKYYNDNQEEYKEYIETWVHEIKTPIASSKLIIENNSNEITKKIDTQLDKV
                     ENFVEQVLYYSKIDNVNKDYIIKKLNLEEVVNEVIKRNYRDFINKNIKLELGEINKTV
                     YSDSKWVEFILNQIVVNSIKYIKSKDGLIKISAKRVENSIVLTIEDNGVGIEERDINK
                     VFEKGFTGENGRVFGKSTGIGLYLCKKLCNKLGLGLILESKVNVGTVIKVSFPIDYNL
                     F"
     gene            complement(6611..7282)
                     /locus_tag="KPE55_002965"
     CDS             complement(6611..7282)
                     /locus_tag="KPE55_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494008.1"
                     /translation="MEKIIIVEDEEVIRDELKSFLERYGYEVMAPTEFDNIVEFILSE
                     KPNLVLLDINLPIYDGYYICRVLRKESNIPIIIVTSRNTDMDELMSMNLGADDFVTKP
                     YKTQILLARIVALLKRSGLNSNVNNTLIFKGIELNLFNATLRYKDKEIELTKNEFKIL
                     SYLVENQGSIVSRDSFMEYLWHTNWFVDDRTLTVNITRLRKKLDEIGIENLIETRRGL
                     GYIIS"
     gene            complement(7359..>8794)
                     /locus_tag="KPE55_002966"
     CDS             complement(7359..>8794)
                     /locus_tag="KPE55_002966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494009.1"
                     /translation="LLTIGRKTEDIKFKSPFVYTITFILCIISLIIAYSMVLKVGLNP
                     KNPKFIMSIVLGVIGTILFFYSLAGFALYIVKKNKNIYFKGLNIFVVKQINSKINTNF
                     ISISIICLMLFLTIGILSTGFGFKSALNAGLKGATPFDASATMIVHEKCKVKNIKESF
                     EKVGIKFNKGDKVAYYDEYINGNKLSDVMQGQYSGRVDFEVAYIKSSQYNEIRKLRGE
                     KSITLSKDEVLITSNATEVVPSIEEYMKNNKIIKIDNKAYNIKNKKVIKDNLRTDFTE
                     TNALTVVVNDKLCDSMEISSSIVNINFASDNNEKSQENFDKRLKSKYSSTSFYEVGTV
                     SGGSRNAMYNDNNGMTSTILFVGIYLGIVFLISSMAVLALQQLSEASDSIDRYVSLKK
                     LGASKNSISKTIFTQTLMYFSIPVGLALVHSVVGIQVANNFISRLNKPDIGASSLMTV
                     GILMLVYAVYFYTTYQGYKNIVIKKLN"
BASE COUNT         2947 a         1476 c          880 g         3491 t
ORIGIN      
        1 aaatttaaat tatctctaaa tagcttaaaa atatattctg atatattgaa aaaagtactt
       61 tcaagatgat attttatata ttatttagaa gttttttatt tattgttata taattaaata
      121 tatgactatt ttgctactac atatataaaa ataaatttat gaaggtgatt ttaatgaaaa
      181 ctatttctaa acaagaatta gaaaaaacac ttgagaaaca ttatttatgg cttaaaaaca
      241 ataatgctgg agaaaaggca gatttaaaag aattaagatt agaaaatatg gacttaagag
      301 gatactgctt aagtaatatt gacttttcgt ggtctgacct tatcaattta aatttagaaa
      361 aagcagattt agaaaactct atttttaata attcttatct ttcggattgt tcattaaaaa
      421 attctatatt aaaaaatgca gacttaagtg gtgcaagttt tagattttgt gacttatcta
      481 actcagatat tagaggagct aatttagaaa attcaaattt agaacatgct actttaaatg
      541 gagtaatatc agatgaaagt acccgttttt ttaaattgta ttgtcctgaa acaggagcat
      601 ttataggcta taaaaaatgt tttaatttta gaatggtaca attattaata cccagcgacg
      661 caaaaagagt gtctgctaca tcaaatgctt gtagatgtga taaggcaaaa gtactgtcaa
      721 ttactagtat agatggtaaa gaatctttta aaagtgctcg tgcatatgct gatgaaaatt
      781 ttatatatag agtaggagaa acgattgtag ctgaagattt taatgaaaat agatggaaag
      841 aatcgactac agggattcac ttttttttaa ctagagagga agctattggt tatctataaa
      901 atgctaaagt tttataaata tataaataca taaacaaaat aacaaaagag tatagattat
      961 aatttcaaaa aaattataat ctatactctt ttttctatag cacataagta cattttattt
     1021 cataaattat aataaattat ttatatctgc actattgatt atttgagaaa ctttatctat
     1081 atgttctatc ttattatcca aactgtcata atacttctta ctctcatctc tatttctaaa
     1141 agaataaact tttttatcca attttaaagc ctcttttata atatctatat tcctcttatt
     1201 tgtctctcct acactaaatc ctgtatctat tataatcttg gaatcattta aatttctaat
     1261 agccaaatcg aaactctcat cacttatagg ctcaaatgga ttttctgtaa acatctttat
     1321 ccccatagtt ctaccaattt cataatcaat atcattttca tgtattattc cagaatacag
     1381 acctatacct tttttagtaa aagctctata aattggtgtt gctttacccc caccaccaat
     1441 tataaaaact tcatttttag atttattact aatttctact gaacctaaaa gactattaaa
     1501 gtttttatca tccaattcat aaagcgaatt tataatatcc tcatctacta catcttcagg
     1561 ctgaccataa gctatgactt tattattttt tataagagca actttatcac aactttttaa
     1621 tgctatgtct atttcatgca atgaaagtat tactgatatt gacttttctt tacttaattt
     1681 tttcagtatg tttatgagtt ccaatctatg ttttatatcc aagtgagttg taggctcatc
     1741 taatataatt atttctggtt cttgaacaag agcccttgca accaacacct tttgtttttc
     1801 tccatcactt agttcatcaa aatatctttt ctttaaatgt aaggcatcta cagactccaa
     1861 tgcttcatct accattatta aatctttctt agatagactc ccaaaaaatc ctgtatatgg
     1921 ataccttcct atattgacta tatcttgtac agtcattaaa tcacctaata atctatttgt
     1981 aagtactaaa gccatttttt ttgataatgc ttttcttgat atacaattta tgtttacacc
     2041 attcaaatat atttcacctt ttattggtga tataagttta gataaggacc ttaatagggt
     2101 agtttttcca gctccattac ttcccaaaag acatagtatt tctccatttt ttacttctac
     2161 atttatatta cttataacaa ctttgttatt gtatcctaca cttaaattat tagttttcaa
     2221 catgtctaag ccctatcctt ccttttcacc aataacatta ctacaattgg agctcctaat
     2281 aaagatgtta tcgtacttat tggtaattct actggtgata aaattactct tgatattaaa
     2341 tcacaaagag ctgtaactat actgcctaaa aatattacta ctggtatcaa aactctatta
     2401 tctgatgttt taaatatcaa ttttgaaatt tgtggcactg caagtccaat aaaccctata
     2461 ggtcctgcaa atgctgatac tattgctgct aatatactag atataaatac tatcaaaact
     2521 ctaaaaaatc ttatatttat gccaatactt tttgcatact cttctcctaa taaaaaagca
     2581 tttaaaggtt ttattatgaa tacagtcatc aaaagaccta atataccaac tattataagt
     2641 atttctacct tttcccatgt aaatccagaa aaacttccca tagaccacat agtaaattct
     2701 tttaatttat cactatttgc aaatgattgt aacacatttg ttataccact acatacatat
     2761 ccggtcataa ttcctattat taaaagagtt gttatattct ttaccttatt tgcaaataaa
     2821 atgactatag acattacaat taaagctcct ataaaagctg ctacaaatat actcattgaa
     2881 tttacactct caaatcccaa tgtgtatcct cccaaaatca tgagagctac tgataaagta
     2941 gaacctgaag atactcctaa tatatacggt tcaactattg ggtttttaaa aaatatctgt
     3001 aataaaatac ctgatactgc taggcatgct ccacctacca tggttgcaag tactctaggg
     3061 attcttatat tgtatattat tttataatgt tctgtattat ccatacctct taatacaaca
     3121 tctattactt ctttaaattc aatttttaca gagccaaatg ctacacacac taaaaaagta
     3181 agtatcatac ttataattaa tactataaat attgttgtat tccttattct cattttattg
     3241 ataaaatcca tttgtatact cctcttagca aagctatatt aactaaaatc tataaaacct
     3301 atctttttgt gtcctctctg tactatttat cataatttat ataatgtttg attttttcac
     3361 ctgtagtacc tgggtggaac atctcaacta aatctactat taattcgtcc gtcttatcta
     3421 ctgattgata ataatctgga gcaaacaccc ataggtttcc gttttttaca acatccaaat
     3481 cagccaatac aggtgcacta gattttatag aatctaaatt tggtgcataa tctgaacttg
     3541 atgaatatac aaatatatct gcatcttttg caacttcatg caattgttca atacttactc
     3601 gcccttcatt tgaatctatt gaagcacatg catttattcc accagccatt tttatcatat
     3661 tatccacata tgaatcttta ggagctatat aagcacttcc ttcatataca gatgcccata
     3721 caactttagg tttagctttg ttttttattt ttttacttac ttcttctacc ttttgtacag
     3781 ttttatttag ttcactagta gccatatctt ctttatcata aaatgcagct attaaacttg
     3841 tccactccat tcttccaaat ggattttgct ctttatactc attgtctaca acataattaa
     3901 gccctaattc ttttaatttg tcaataaatt ttgtatcagc ctctgataaa ccactactca
     3961 ctatagttaa gtcaggattt aagtctacta ttttttcata atctggtgct aaatttgaac
     4021 caacgtaatg tatttttcca tctttcatta aatctttaat ttctttaata gtccagtact
     4081 gctcttctgt tgttacggct tttatactat ccagttcatc tattgttctc agagaacaag
     4141 cctgtgttgt agatgctatt aatacattgt ccacaggcgt tctaactata ttagcatttt
     4201 tatatttttc aggaatttta atcttctttg ggattaaaat cagttctcga ttatctccat
     4261 cagttacttt tttaattcca tcttctaaat cttcaacact aaatccacta gcatatttta
     4321 attctaattt cttaccagta ctagcttgtt tattctcttt gtcactttta ctacacccta
     4381 taagacctcc taccataaaa attaccatag caattaaaaa aaactgcttt aaaacatttg
     4441 atttaaattt catctctttc actccaatat cttatttttt atgagtggct aaaaaacttt
     4501 aataagatat accttttaaa gtatatctta ttaaagttat attaaaccat ctttaacaag
     4561 tataactttt taataaaatt atagtgaatt ttgatttcac atgcaattaa ctacttatac
     4621 acttaactac ctacgacaac gtttgaagtg gcatcttctt tgtcaaaata gctattgtta
     4681 ttagtatctc caagctaata ggatagttcc caacacttac gattagcatc tgagtcacta
     4741 cgttggcaaa ctaatagaaa tataacattt atttgatgat gctttagata cagtacaact
     4801 ctatcaaatt gcctatgttg ttttatccta atcattgatt ttaatttaag cagttttata
     4861 tacccatttc tatatgcaca atatcttttg agtaacgtgt tgacaaacta ttttggttag
     4921 tagtatagct atgatagtta attttctttg atagataagt ccgttacatc caaaattctt
     4981 aggaaaatat tccttttatt cactgtttgt ataaacctgt acttataagc tcttaacata
     5041 ttatcactat tcttcttgat tttctatgta ttgttccaaa tcttccaatg ttgcaccacc
     5101 agttgttact aaacaataac ttttactcca aaagtattct ttccataagt atttgcggat
     5161 acttggaaac tcattcttta tccttctgct tgatgcagat ttataggcat ttaataactt
     5221 ggaaatttct gtattaggct ttgctttgaa taataagtga acatggtcaa cataatgatt
     5281 ccattctatc aacaccaccc cataatttgg tgcaatatct acaaatatat cttttagtct
     5341 atcagatata gaatcatcta ttacttttct tctatatttt atacacataa ctaaatgata
     5401 atgcaataag aagactgaat gattattact atctaatttc attataatta cttcccttca
     5461 gcttttacta ctaaattaat gatataatac gtttttatga aaatcaagta aaaaaaagaa
     5521 cctatcaatt atgcttacat tcttaacttt cttaaataaa tacaaaaaac tgacaactaa
     5581 aaattgtcag tttttataac tatacactaa aataaattgt aatctatagg gaatgatacc
     5641 tttatgactg taccaacatt tacttttgat tctaatatta aacctaaacc taacttatta
     5701 cataactttt tacatagata taatcctatt ccagtacttt ttccaaatac tcttccattc
     5761 tctcctgtaa accctttttc aaatacctta ttaatatctc tctcttctat acctactcca
     5821 ttatcttcta tagttaacac tattgagttt tctacccttt ttgcacttat ttttattaaa
     5881 ccatcttttg actttatata ttttatagaa tttaccacaa tttgatttaa tataaactca
     5941 acccatttcg agtcactata tacagtctta ttaatttcac caagttctaa ttttatattt
     6001 ttgtttataa agtctctata gtttcttttg ataacttcat ttacaacttc ttcaaggttt
     6061 agctttttta ttatataatc tttatttaca ttatcaattt ttgaataata taatacttgt
     6121 tccacaaaat tttcaacttt atctagttga gtatctatct ttttagtaat ctcattacta
     6181 ttattttcta taataagttt actagatgct ataggcgttt ttatttcatg aacccaagtt
     6241 tcaatatatt ctttatattc ttcttgatta tcattataat atttaatatt ttcgtgcatg
     6301 tttctactta ttacttttaa aatatcattt attttttctc cagttacaaa atttacatca
     6361 tcaataactt ctggcaacaa atatttttta tctaaagcat ctaaaatatt atctatagaa
     6421 ttaaagtact tcttatattg taaataatca attaacatat aagtaaataa tggtaaaaac
     6481 catatacaaa ataacataaa tattaaaacg acatttattt tactataaag catgatacct
     6541 gatgcaatta aaaatataat taaattagta cctagaaaaa caactttatc acctatatac
     6601 tctcttaatc tcatgatatt atatacccta aaccacgtct tgtctctatc aaattttcta
     6661 tgcctatttc atctagcttt ttacgtagcc ttgttatatt gacagtaaga gttctatcgt
     6721 ctacaaacca atttgtgtgc catagatact ccataaatga atctctagat actattgaac
     6781 cttgattctc cactaaataa gatagtattt taaattcatt tttagttaac tctatttctt
     6841 tatctttata tctgagtgtt gcattaaata aatttaattc aataccctta aatattaaag
     6901 tattatttac attcgaattt aatccacttc tttttaacaa tgcaacaatc cttgctaaaa
     6961 gtatttgtgt tttatatggc tttgtcacaa aatcatctgc tcctaaattc atgctcatta
     7021 gttcatccat atcagtgttt ctacttgtta caattattat tggtatattc gattctttac
     7081 gtaatactct acatatataa tatccatcat atattggcaa gtttatatca agtaatacta
     7141 aattaggttt ttcacttaat ataaactcta ctatattatc aaattcagta ggagccataa
     7201 cttcatatcc atacctctct aaaaagcttt ttaattcatc tctgattact tcttcatctt
     7261 ccactataat tattttttcc attaattttt caccctttat tttcaaaaaa tctatttaca
     7321 gtatgatttt tactgtaaac agattttagt tagttgtatt aattaagctt ttttataact
     7381 atatttttat acccttgata agttgtataa aaatacactg catatactaa cattaaaatt
     7441 cctactgtca taagtgatga agctcctata tctggcttat taagccttga tataaaatta
     7501 ttagccactt gtattcctac tactgagtga actaaagcta gtccaacagg tatgctaaag
     7561 tacattagag tttgtgtaaa tatagtttta cttatactat ttttacttgc tcctagtttt
     7621 tttaaggata catatctatc tatactatca ctagcctcac ttaattgctg aagtgcaagg
     7681 actgccatac tacttattaa aaatactatt cctaaataga ttccaacaaa tagtattgta
     7741 gaagtcatac cattattatc attatacata gcatttcttg atccacctga tacagttcca
     7801 acttcataaa aacttgtaga actatacttt gattttagcc ttttatcaaa attttcttgt
     7861 gacttttcat tattatctga agcgaagttt atatttacta tacttgaaga tatctccatg
     7921 ctatcacaca atttgtcatt tacaactaca gtaagagcat ttgtttctgt aaaatcagtt
     7981 cttaaattat cttttattac ttttttattt tttatattat atgctttatt atctattttt
     8041 ataattttat tatttttcat gtattcttct atcgaaggta ctacctctgt agcattagat
     8101 gttattaata cttcatcttt tgataaagtt attgactttt cacctcgtaa ctttcttatt
     8161 tcattatact gtgaagattt tatatatgct acttcaaagt ccactctacc agaatattgt
     8221 ccttgcataa catctgaaag tttattacca tttatatatt catcatagta agcaacttta
     8281 tcacctttat taaacttaat tccaaccttt tcaaaagatt cttttatatt cttcacttta
     8341 catttttcat gtacaatcat ggttgcactt gcatcaaatg gagttgcacc ttttagtcct
     8401 gcatttaaag ctgatttaaa accaaaccct gttgatagta ttcctattgt taaaaataac
     8461 attaaacaaa taatagatat tgatataaag tttgtattaa tcttactatt tatttgcttt
     8521 actacaaata tgtttaatcc cttaaagtaa atgttcttat tcttcttaac aatatataat
     8581 gcaaagcctg ctaaactata gaagaataat atagttccaa ttacacctaa tactattgac
     8641 ataataaact taggattttt aggatttaat ccaactttta aaaccatgct atatgcaata
     8701 attaaggaaa taatgcataa tataaatgta attgtatata caaatggact tttaaatttt
     8761 atatcctctg tttttcttcc tattgttaat aaat
//
LOCUS       DAELNY010000159         8781 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.161, whole genome shotgun sequence.
ACCESSION   DAELNY010000159 DAELNY010000000
VERSION     DAELNY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.161"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(61..1125)
                     /locus_tag="KPE55_002967"
     CDS             complement(61..1125)
                     /locus_tag="KPE55_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG0494010.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1387..2058
                     /locus_tag="KPE55_002968"
     CDS             1387..2058
                     /locus_tag="KPE55_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494011.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGYGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            2060..2968
                     /locus_tag="KPE55_002969"
     CDS             2060..2968
                     /locus_tag="KPE55_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0494012.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            3045..3731
                     /locus_tag="KPE55_002970"
     CDS             3045..3731
                     /locus_tag="KPE55_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494013.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            3728..6283
                     /locus_tag="KPE55_002971"
     CDS             3728..6283
                     /locus_tag="KPE55_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0494014.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGITKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(6477..7676)
                     /locus_tag="KPE55_002972"
     CDS             complement(6477..7676)
                     /locus_tag="KPE55_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG0494015.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            7795..8682
                     /locus_tag="KPE55_002973"
     CDS             7795..8682
                     /locus_tag="KPE55_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0494016.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
BASE COUNT         3382 a         1113 c         1270 g         3016 t
ORIGIN      
        1 atatttaata aagaacctgt cctatgataa attctagtac agattcttta ttaaataaaa
       61 ttatttcaac caagatggat ttccaggtat aaatattctt ttaccaaatt ttgaagtata
      121 ctcatctaaa gctttttgaa taagctcatt attaccctta aatgaagtag tattctttat
      181 agtttcgaga ttttggctaa atgggcaagc agatatacac ataccacaat ctgtacctaa
      241 atatctccat ttaatatagc atgtctcatg ttcaattacc caattatatt ttccatcatc
      301 accaatttta atatcattag aaagcgaatg agaagggcag ttaaaagagc attttttaca
      361 gaccttacaa aaatcttcta gtccaaaatc aacatattca tcttctaaaa gaggaatatc
      421 tgtagtaaca acacctaatc taagtctaga gccataatct ttattcgtaa gtatagcatt
      481 tcttcctata tctcctaaac cagcatcacg agctataaaa acaggcatta ccaaataatt
      541 agcatccata tgatttctag catcatatcc taaatttctt atatagtaac ttaaaatcat
      601 gccgacgata gatgcatcca catagcactt tgatgtttct ataacttcac atatcattgg
      661 agctctattt atcatttctt tatccatctc acatccaaat acaattgcat acttatgagt
      721 tagattaatt tcttcaccgt aattttcttc atgtctaccc ctatttgtat aaatatgata
      781 gtcttttaat ttagttatac caactacaga agctccatac tgctttgcaa gacccttaat
      841 tttttttgtc attgactttg catctacatc tactttagta tcagaaactt ttccttcgca
      901 taaagatttg atatcagata aaaaatcgaa tgcactactt gccattggag agtttaactc
      961 attgtaagtc attgttcctt cactacataa atttggtcta tttcttatac tatcatcaat
     1021 ttcttttttg tcaggatttt tcttataata atcattataa gcagtactat cttttttgta
     1081 atttgctctt gcaaacatag tatttctctc atcaaccctt ttcaattcta ttccccctag
     1141 taaaaatata tttaaaaaat ataactttat tgttgtataa tttaattata taagaataaa
     1201 gttatatttt caatttaagc gtaattaaat tgggaaatat ataactatat gcttgattat
     1261 gatgttttta ttttgaaaac aaatttaaat acaatctttt tagtgaaata aataaaatta
     1321 aaatattttt agtatgataa tgcaagaaaa taattattta tctaaaaaat ctaaaggaga
     1381 gtaaaattga acttattaat tattgaagat gatataaact taaatgaagg tcttttttat
     1441 gcatttgaga atgatggatt taatgtattt aaggcttata caaaacaaga aggattaaat
     1501 atatttaata gtaaaaatat agattttata atcttagact gcaatcttcc agatggatat
     1561 ggatttgatg tatgtcaaat aataagggaa aagtcagata taccaattat aatgcttact
     1621 gcaagagact cagaaattga tgaagtaaaa ggattagaaa tagggcttga tgattatatt
     1681 acaaaaccat tttctttatc tgtattaaag gcaagagtca aagtagcaat aagaaaaaaa
     1741 tcgaataata aagtaatata ttccaatgga ataaaattgg accaaaagtt actaaaagta
     1801 tataaaaata aagaatgttt ggaacttagc tctgtagaat ataagcttct aagttatcta
     1861 attgaaaata aaggtcaaat attattaaag gaacaaattt tacaccatat ttgggacagc
     1921 gaagaaaatt atgttgatga taatattgta tctgtaaata taagaagact tagagtaaaa
     1981 gtagaggatg acccatctaa tccaaaatat ataaaaacag cttatggaat gggttattta
     2041 tggaatgagg tagaataata tgtttatatt agcctttata atatgtacag ttgttatatt
     2101 tatagtatct actaaaataa atcgaaaaag atatgatgaa ttaaatttga tgttaaatca
     2161 aatattggaa ggtaaagaag ttacttatcc agatacaaag gatacaagag cgtcaaaaat
     2221 ttctcaccaa gtaaaaaaaa ttaaggatat gatagaaata gaggtagaac agtcaaagtc
     2281 agaaaaagaa gcgattaaag gtttaatatc aaatatgtca catcaattaa aaactccact
     2341 ttcaaatata accatttatt gtgaacttct ggagaatata aatatttcca cattacagaa
     2401 gaaggagttt ttacaaaaga tgaaaaatga aacttttaag attgactggc tgttacaatc
     2461 cttatttaaa atgactaaat tagaggatgg agttatagaa tttgaagtag aagaactact
     2521 aattaaagat actcttattc aaagtatcag tactatcttt aataaagcag aagctaaaaa
     2581 tataaggata aatctcgaac cattttcaga cataaagtta gttcataata aaaagtggac
     2641 catagaagca attgtaaatg ttttagaaaa tgcaataaaa tactcaccat cagatagcac
     2701 gattactatt tctgtaataa aaatggaact atatacgaag attactatta aagatgaggg
     2761 aataggtatt gatagtagag aactaaatga tatttttaaa agattttaca gaagtaaaaa
     2821 tgtggaaaac caaaatggca cagggattgg tctatatctt acaagattaa ttttagaaaa
     2881 agaaaatgga aacattatag tagaatctaa actaggaagt ggatgttgtt ttagtatctt
     2941 cttacagaat tgtaagagtt taaactaatt ttgtaggatg agtgtaatta tcgtcactta
     3001 caatatttta tagaacatat atctgtattg aaaggagaaa ttaaatgagt atattagtga
     3061 cgaataattt agtaaaacac tatggaaaag gtgagactaa agtaaaagcc ttaaatggag
     3121 taagtataga aattgaacga gatacattta ctgctatagt tggaacttct ggttctggaa
     3181 aaagcacatt gttaaatatt ataggtggat tagataatcc aacttcaggt gatgtaataa
     3241 ttaaaggaaa gaacatatct aaaataggaa aaaaagattt gacagtattt agaagaagaa
     3301 atataggatt tatatttcaa aactacagtt taatgcccgt attaaacact tatgataata
     3361 tagctcttcc tgttacatta gataaaggaa accatataga ccatgaatat attgaaatgc
     3421 tgatgaagac attgggaatt tgggataagc gtttaaaatt tccaagcgaa ctatctggtg
     3481 gtcaacaaca gagggtagca attgctagag ctttggcaaa taaacctgcg cttatattag
     3541 cagatgaacc aacaggaaat ctcgatagta aaactacaat ggaagtggta tgtttattaa
     3601 aagaaagtag tgcaaaattc catcaaacga tacttatggt aacacacaat gaaaatatag
     3661 cacaagtatg cgactcaatt atccatattg aagatggagt tgtggtgaat actggtggtg
     3721 aagtactatg atgtctctag taaaattttc aattcaatat ataaagcatt ataaaaaaca
     3781 atctatatct atagttttaa gcatagtact atcagtagca ttattgacgg gaattggttc
     3841 tttagttcat agtgcagaca aaagtagaat tgaaaagata agggaagata gtggagatta
     3901 tcattatttc tataaagtag ataaggaaca acttaataaa attaaagaaa ataaaaaatc
     3961 aaaggaatac actgtagata gacttggcat tacaaaacct aaaaatcaga tagatgaacc
     4021 ttttattata gaatttttaa atgctgattc ttcatattta gacatgacag gtagaaaatt
     4081 attaaaagga actcttccta gtaaagatgg agagatagta cttgacacat attctctaaa
     4141 caatttaaat gtaaacaaag aaattggaac agaaatcaat ttagatggta aaatctataa
     4201 gttatgtggt attttatcaa gtctttcaaa cccagaaact gctctggagg gatttgtaaa
     4261 cgattatttt cagcctaaag aaaaggattc ttatatagtt tatgtaaaat atgatgaaaa
     4321 taaaaatatt aaaaagcaaa attataaatt tatgaaaaaa ttcaacatat caaaagaaaa
     4381 aacttatata aactggcctc tttcagttga atttggagta aaaccaccaa aaaatataga
     4441 aaaatataat atatttgagt tcttaaaaaa tttagaacta aatacaaatg caattatatt
     4501 acttgttgga gtgtttggtg cagcagcagt atatagtatt ttccatgttt caatacttca
     4561 gagaatatca caatatgggg ttttagaagt attaggcgct aataataaac aacttttatt
     4621 tctactattt ttagagttat ttttactttt tattgttgga tttcctatag gttgcttctt
     4681 aggtattgaa gtggcgtcta caatatatga acaatttcca catatatttt tgagtagtga
     4741 tattgttcca ggagcatttt ttataagtga aaaatctatt tatttaggct ttttatttat
     4801 cttattgttg ttaattttaa ttgctgtacg tgttgtacat cagttgaata aatactctag
     4861 tatagaatct atgaaaaact ttaagattac taataagaaa aagagaatat tagaaagtgt
     4921 aaaacactct aacatgacaa aagtattatc tcataaatac atgactcaaa aaaagggaat
     4981 gtttattgga attttgtttt cattatcact tggtggaatt atatttctat gttctagtta
     5041 ctctatacag cttactaaaa ataataatga acttacaatg aaagctgatg atggtttaaa
     5101 ttctgactac caaataacta tgcaaacaac agattttgat gtaggtatac caaaagataa
     5161 aatttcaaga ttaaacaatg ttgagggaat atcaaaagtc tatccagtaa gatatttatt
     5221 tggagctatg caaattaaag aagaacaact attttggaaa aatttcttta aacctcttga
     5281 aaaagattac aggataaaga ctttttttga tggtatttgt acaaagcaaa atgaaggtgg
     5341 ctatctttta aaaacaaata tttatggtta taacaatggt atgttagaag gattaaattc
     5401 ttatattata gatggggaga ttaataccca tgatatggtt aaaaataata aggttatagt
     5461 tagactacct atggatggaa ctggtatgta tgatgcagtt gatataaatc caggagatac
     5521 aataaaagtt aaagtgccaa agacaatgaa accaacagat gaaattgtta aatttaaaga
     5581 agaaaataag aatgattata caacaaaaga atttgtagtt gctgctactg taaaacgtgt
     5641 gatggcaaat aacatatatt tcattggtga ttatggtatg gatatagtta tgacgaatgg
     5701 gcaaatgaaa aataactttg atatcgaaaa ttataattca gtaagtataa aaaagacaga
     5761 agatagtaac agtgaggaat tgtctgagaa cataaaaagt gttgtaagtg atgtaaaaag
     5821 atgtattgta acagattata ccattgctat tgaaaaaaat aatacctact taaatcaaaa
     5881 actcttattt atatatggta ttgtatttgt acttcttgca ataagtttgt tccatataat
     5941 caatacagta agctacttaa ttttttcaag aagacatgag tttggaattt taagagctat
     6001 gggtattacg gataataagt ttttacttat gatgataaga gaaggttttt tatatggatt
     6061 ctatgcaagt attataatgg taattggaag tgttattgga caatttatga tttattttat
     6121 ggtaaaacgt gtatacttat atataaatcc aattcttaaa ataaatacac ctctatacat
     6181 aggtatgatt attttaaata tcactattag tatagttgct gtaattattc cagtaagaca
     6241 aattttgaag agtgatatta tttcagaaat aaataagaat tagagttata taaattttta
     6301 agattatata actttctgac aatcttgttt aaattattta aatatatctt atctgtgaac
     6361 attaaataaa cctcttaata gatataactc ttgaaaagtc atatctatta agaggtttta
     6421 ataaattttc atgttaatat atataatcca aaaaatttaa atcttattaa aacatactat
     6481 tttgtatagt tatcgaaata tccttgtatg taaataatag gagtaccctt atctccactt
     6541 ccagatgtta aatctgctaa agaacctata aggtctgtta atcttcttgg agtagtacct
     6601 tgagaaacca tgtttcctgt taaatcatca gatttattat ctttttcaac tatgtatttt
     6661 gaaattgctt cttttaactc atcaccagat aagtcagcaa aatcattatc tgctaagtat
     6721 tttaatttta cttcgtttgg agttccctct agcccttttg tataagcagg agatactact
     6781 gggtcagcta attcccatat tttacctaca gggtctttaa aagctccatc tccataaacc
     6841 attacttcta catctttccc tgttatttct tttatattac cttggatttt gtttacaact
     6901 tcatcacaat ttatagggaa taattttact gtttcttctg tagctttgtt tgaacctaaa
     6961 agtccatact ggtcattgta accactacca tctacacttg ctgtcattat atcatcaagt
     7021 gaatatactt ttttagctcc attttgtctt aaaattctct ttgttctttg tcttgtatgt
     7081 atatcacaag ttaaaatact tgttgtatag ttaagaatag tttttggatt gtttgcaaat
     7141 actatttcta cctctgcacc acaatccttg attaaattct tataataatc aacataatct
     7201 acaccagtaa atgtatgctt gttatatcca aatagttctc tgtatttttc ttctgttaaa
     7261 acatctgacc aaggatttat tcccttatca tcaagttcat caatacttat taagtgattt
     7321 ccaacttcat cagaagggta gcttaacatt aatacgactt ttctacatcc ttttgcaatt
     7381 ccttttaaac atatagcaaa acgatttcta cttaaaattg ggaatataac tccaacagta
     7441 tcatctccaa atttatcttt tacatctttt gcaatattgt caacgtgagc ataatttccc
     7501 tgtgctctag ctacaacagc ttctgtaacg gcaattacat ctttgtccct aacttcaaag
     7561 ttttcacttt ttgacgcatt taaaacagaa tcaacaacta ttttaactaa gtcatctcct
     7621 tgtcttataa taggtgctct aacccctctt gaaacagttc caactaatct atccattatc
     7681 taaaatcctc ctaaagaatt aatattgtca tattagtacc atgtatatta taattcaaat
     7741 ataattataa gtaaaataaa taatactaat atttgacata aggagtaatt atatatgaat
     7801 ataaaacttg aactttataa ggtttttaat gcagttgtaa ataataagag tttttctctt
     7861 gctgctaagg aattgtttat gtcacagcca gctgtaagcc agtcaataaa gcaattagaa
     7921 gaacaattag atactttatt attttataga aacaataagg gagtaaaatt aactccagag
     7981 gggaaaattc taagtgaaca tgtcactact gcattaaagt taatatcatc aggagaagat
     8041 agaataaata aatttaaaaa attagaatac ggttctctga aaataggagt aggagataca
     8101 gctgcacgtt ttttcttgtt aaaatattta gaaatttttc ataagaagta tccacatata
     8161 catgtatcta caataaatag aacgagtaga gaacttatat ctttactcaa agatgggaat
     8221 atagatatag caattataaa catgccaata gaagatgata cactaaatat tgttgagtgt
     8281 attgaaatac atgatatatt tgtatgtgca aatgattaca tagaatacaa aggtaggaaa
     8341 atatcattgg aagaattaaa cacattgcct ttaataatgc tagaaaataa agcaaattca
     8401 agactttatg taaatgaata ttttttatca aaaggtataa aactaaaccc ggatatagaa
     8461 ttgggttctc atgaattact tttagaattt gcgtatataa atcttggagt gtcttgtgtt
     8521 atagaagaat ttagcataga ttatttggaa aatgagaaat tgtttaaatt agatataaaa
     8581 gagccaatac ctaagagaaa tataggttac tgccatttaa aagatatatc tttatcctta
     8641 gcaaccaaag aatttttaag catgatatct aataatatat aatttagaat acatatttca
     8701 caagtaattt aatataaaaa tagagataaa ttcgtaaact tgaaagattc atatagtaat
     8761 tagtttttat taaaaataaa a
//
LOCUS       DAELNY010000160         8674 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.162, whole genome shotgun sequence.
ACCESSION   DAELNY010000160 DAELNY010000000
VERSION     DAELNY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.162"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..512
                     /locus_tag="KPE55_002974"
     CDS             192..512
                     /locus_tag="KPE55_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG0494017.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            650..1294
                     /gene="trmB"
                     /locus_tag="KPE55_002975"
     CDS             650..1294
                     /gene="trmB"
                     /locus_tag="KPE55_002975"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG0494018.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1423..2169
                     /locus_tag="KPE55_002976"
     CDS             1423..2169
                     /locus_tag="KPE55_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494019.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2298..2576)
                     /locus_tag="KPE55_002977"
     CDS             complement(2298..2576)
                     /locus_tag="KPE55_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494020.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2851..3297
                     /locus_tag="KPE55_002978"
     CDS             2851..3297
                     /locus_tag="KPE55_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494021.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3567..4019
                     /gene="accB"
                     /locus_tag="KPE55_002979"
     CDS             3567..4019
                     /gene="accB"
                     /locus_tag="KPE55_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG0494022.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            4035..5396
                     /gene="accC"
                     /locus_tag="KPE55_002980"
     CDS             4035..5396
                     /gene="accC"
                     /locus_tag="KPE55_002980"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG0494023.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5411..6265
                     /locus_tag="KPE55_002981"
     CDS             5411..6265
                     /locus_tag="KPE55_002981"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG0494024.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6286..7230
                     /locus_tag="KPE55_002982"
     CDS             6286..7230
                     /locus_tag="KPE55_002982"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG0494025.1"
                     /translation="MIENNSEKIKILENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7419..7679
                     /locus_tag="KPE55_002983"
     CDS             7419..7679
                     /locus_tag="KPE55_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG0494026.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7832..8203
                     /locus_tag="KPE55_002984"
     CDS             7832..8203
                     /locus_tag="KPE55_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0494027.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8233..>8674
                     /locus_tag="KPE55_002985"
     CDS             8233..>8674
                     /locus_tag="KPE55_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494028.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLK"
BASE COUNT         3384 a          906 c         1486 g         2898 t
ORIGIN      
        1 ttttatttgt ttactaagtt tatgataggg tatttattta aggtaataaa atattatata
       61 aaatatttta aagccttagg taatattagg tagaagtttt agtaaaatat gcaaaaacta
      121 attgattatg taccttgtat gggtatataa tagatattgt tttaataaaa tatttgaaag
      181 ggaatgattt gatgaaaatt actttaccag aaacagctat tgatacttta aaaagtatct
      241 taaaagacaa tcaagataaa cctaataata taagagtata ctttgcaggt gttggttgtg
      301 gaggtccttc ttttggatta gctttagatg agaaaaaaga agatgactta acttatgaag
      361 ttggagaatt acaatttgta atgagctctg atgaatatag tcagtatgga gatataataa
      421 tagaagatac aggatttgga tttagagtta ttcctgaaaa tatgaaagat caaggtggag
      481 gaggatgctc tggttgttca gggtgccact aagattaagt ttttagtaag tttagaactc
      541 tctttataga aataaatttc tattttaaga gagttctttt tattttttgg tgagataatg
      601 tataataaaa atgtatttta ctaatttttt atatgtaagg agaattattg tgagaagaag
      661 acgaaaaaaa ggtgcagatg aaaagctttt aagttataca aaatatgttt taagagacga
      721 tatagacaaa ttaaaaggca agtggaattt aaagtttaga aatgataatc caatacatgt
      781 tgaatttggg actggaaaag gtaagtttat aacaacttta gctaagcaga accctgacat
      841 aaattatatt gccatggaac ttaaggaaga agttctttta aaggctgttg aaaaagcaga
      901 tgcttctaat ttgaataata tactattttt atggggagat gtaagcaata ttttggatta
      961 ctttgaagct aaagaattat cgagaattta tataaatttc tgtgacccat ggcctaaaaa
     1021 tagatggagt aaaagaagac tcactcattc tggattttta gaaatgtaca atagggtttt
     1081 ggaagatgat ggcgaaattc actttaaaac agataatgaa aaattattcg agtttagttt
     1141 aaatgaaatt gctgcaaata attggctatt aaagaatatt tctcttgatt taggaaatag
     1201 tgagtatgaa aataatgtta caacagagta tgaagacaaa tttatgtcac aaggtatgcg
     1261 aatatttaga tgtgaagcta aaaaacgtaa ctagtaatat gtttataagt ttaatagtag
     1321 aaatttattt taatttatat taataaagtg tacgaaagta ggtatatata acaaggaaac
     1381 agaaccctct catagaaatt aaattattat aataaaaaat ttatgtggga gggtactatg
     1441 aatttagcta atttaatacc atttattatt tctgcagcag ctttaagtcc aataaattca
     1501 aatgataagg ttttgagcga aaatcaactt aaagatattt ttcctgatgc aaatttaaga
     1561 gcagttgtaa aaagatatat aaatccagat gagatgacta taagcaatat aaaggcttta
     1621 gatggagaat tttatgcaac tggtgagagt atttcaaatt taaaaggtat atcatatctt
     1681 gaaaatgtag acaattttat tttttggaat aataatataa aagaggttcc taaggaagct
     1741 ttaagtctaa aagatatgga ttcaataaat cttgccaata attatttgat tgatgacgat
     1801 gtagtaaatt ctctttctca taatggtgtg gatgtaaatt gcgatttaaa ttttattgat
     1861 acaaaagata atcagtataa actggactct aaatatcata atgttgatat agcaaaaggt
     1921 gaaagtattg atttgagaaa aataattacg aagaaaattg atagttatta taaatactgg
     1981 gaagttactg ataaccttcc aaaagattta aattttattg tatctgtaag tgataagagt
     2041 gtactttcat gtgaagatat gataattaaa ggtaataaaa caggtcaagc agtggtaaaa
     2101 gttatactaa gcgacaaaaa tgatttaaat acatctcaag aagttctaat tactgtcaat
     2161 gtaaaataat ctgtcaactt attcaatcat tctattttca atttaagtgt ataatcaaaa
     2221 taaaggttgt tagtaacaga acgctaatat tagtgatttg ttactagcaa cctttaaatt
     2281 ttatagaaat agattatcta aaaaattcca tttcttctgt tagtagccca tattcctatt
     2341 aaaattatca gtgaatatat tatatacaaa actattctta tatattggac atctaatcca
     2401 aaaaattcaa tgttactctg tgaggtatgc aacatcacaa ggactgcaga tataccaata
     2461 agcaaaggat tgattactat ttgcttatca gttcttaaaa tcattatttt acccatccaa
     2521 attattgata atataaaaaa taaaatttta agaccaaatt ctaatgtatc taccaaagcg
     2581 aaaacatccc ttcatttata ataaattttt cagctcaaca aaatataaaa ccctacaaac
     2641 aacttttgaa tccataataa gataacttga ttaagcacta tgaaataagt actataaaac
     2701 ttcatctaca ttaatatata ctacactata cttcaaattt cttaaacata aattaatgaa
     2761 attaacaaaa ttttattcag aaacatataa agatatattt tactatttaa atgcatatca
     2821 ttatatatac agttttagga ggtgaggtat atggaatcta aattttttaa ttggtacact
     2881 caatccttag ggggaacttt tggcattata tcatgtatat gcgcatattt aaatggatac
     2941 atgtttgttt atacaaatag tgctagcaaa tattttgaaa gtataggatt tttgggaata
     3001 atttcaagtt atacactact tccactttgt atgcttactt tgatttttgg agttatatat
     3061 tcttatactc cagaaacatc cgacaaaagt atttttaata tacctttttt tactttaaat
     3121 aaaagattaa tacttattac agtagttttt ggttttatag gtgctagagt ttactttata
     3181 attcctgcaa ttctaataac ttttaaccta tatagcaatg ttttgatagc taaattaaag
     3241 tacatcctat gtaaaaataa agaaaatgat actaatttga aagaaagcga agtctaaatt
     3301 gcataatatt ggtatttgtc ttaaaaattc ccgaggtatt aatttgtatt ctcttgtatt
     3361 ttgcaaaaaa tattgaattt tattcatttt atacttataa tttatattaa taatggaaaa
     3421 ttaaaacaaa tataagttac tggttgttaa tttaaaatta aaagatatat tgatgtgttg
     3481 attctctagt cagcactttt tcttttataa atttgttttt tgtgatataa tattagcacc
     3541 aggtaataat acaagggggt aacaaaatga acatcaatga aattaaggaa ttattaaagg
     3601 ccatagactc aactaactta gaatatgtaa aacttgaaag tagcgattta agattagaag
     3661 tatctaaaaa agctcaatct acaagttcac ctgttttaag tgtacaacaa gaatcagtag
     3721 tggatctttc tttagaaaag ccagttgtaa acgatacacc tgtcacatct aatgagaatc
     3781 tatcagtagt agtagctcct ttgatgggaa cattctatga ttctccaagt ccagatgcag
     3841 atagctttgt taaagttggc gatgtagttg aagaaggcga cactttatgt atattagaag
     3901 caatgaagct tatgaatgag ataacaagtg aaattaaagg tgaaataatt gaagtattag
     3961 ttagtaatga agaactagta gaatataatc aacctttatt taagataaaa ccattgtagt
     4021 aggaggtatt taagttgata aaaaaaatac tagttgcaaa tagaggcgat atagcagtta
     4081 gaatcatcag aacatgtaaa gaactaggta taaaaactgt tgctatctat tctgaaatag
     4141 ataaagattg ttttcataga tatatagctg atgaatctat ttgtatagga ccaaataata
     4201 taagtaagag ttataataat atagaaaata taatctatct tgctcttaaa ttaaagtgtg
     4261 atgctattca tccaggcttt ggatttttat cagagaatcc tgaatttgca aagcaatgtg
     4321 aagataataa tattattttt ataggtccaa caagagaaca aatgattttg atgggagata
     4381 aatctagagc aagagaaact atgatggaat tgaatatccc tgttgttcct ggttcagaat
     4441 ctgttttgaa aactaaggaa gaagctttag aagttgcaag agaaattgga tatcctgtta
     4501 tgataaaagc ctcaagtgga ggcggtggaa aaggtatgag aatcgttaga aaagaagaag
     4561 aacttttttc aaattttgat atggcaagtt cagaagctct agcagcattt tctaatagtg
     4621 atttatatat ggaaaagttt atagaaaatc ctagacatat agaagtacaa gtatttggag
     4681 ataaacattc aaatgcaata catttaggtg atagagattg ttctatgcaa aggcgaaatc
     4741 aaaaagtaat agaagaatct ttaagtccat acctttcaga tgaagaaaga caaaaacttc
     4801 ataaaattgc agtagatata gtaaaaggag taggttatat cggtgcaggt actattgagt
     4861 ttatagttga caaagataaa aacttttatt ttatagagat gaatacaaga atacaagttg
     4921 aacatccagt tacagagatg gtaacaaatc ttgaccttat aaaacttcaa atatctatag
     4981 ctaatgggga taaaattcct tttaaacaag aagatattac ttttagaggt catgctattg
     5041 aatgtagaat aaatgcagaa gattcatcaa agaattttgc tccatcacca ggtaagatag
     5101 aatcattaaa tttacctgga ggttttggtg ttagatttga tacttttgta tatgcaggat
     5161 atactatacc acctttatat gattctatga taggtaagtt aatatgttgg gctgaaacta
     5221 gagaagaatg tataagtaga atatatagag ctctagacga aattattgta gaaggtatta
     5281 atacaaatgt agaatttcaa aaggcacttg taacaagtga ggagtttaga gaagatactc
     5341 atcataccaa atttatagaa gatgtcttta tgaagaaaga atttgctaca ttataaggag
     5401 taaatagatt atgataaaaa aatttttatc atcaaaagaa tctaagtatg ttacgatttc
     5461 tttagatgac aactttaaaa agaatagtgt agatgataaa ttttggacct attgcaaagg
     5521 ttgtgatagt catgtattta gaaaggacat agaagaaaat tcatttgtat gtccaaaatg
     5581 ttcaagacat tatggcttaa gagcaagaaa aagaataaat ttattgattg ataaaggaac
     5641 ttttatggag tttaattctg atgtagaatt tcaaaatcca ctaaattttc ctaaatacaa
     5701 agaaaaagta gactcttata aggaaaaaac aaaagagtca gaagctgttg tcacaggtta
     5761 tggaagaata aatggaatta aaacagttat ttgtgtaatg aatcctgatt ttatgatggg
     5821 aagtatgggt tctattgttg gggaaaaaat aacttattca atagagtatg ctgctgaaaa
     5881 taatttacct ataattattt gttctgcttc aggaggagct agaatgcaag aaggtatggt
     5941 atctcttatg cagatggcaa agacttctca agcactttct aagcttgaag aaaaatcatt
     6001 gccatatata tcagttctaa ctgaccctac tactggagga gtaactgcta gttttgctat
     6061 gcttggtgat attataattt ctgagccaaa tacgttgata ggttttgcag gtccaagagt
     6121 aatagagcaa acaataaatc aaaaacttcc tgaaggattt caaacttctg agtttttact
     6181 tgaaaaaggt tttattgaca tgatagtaga tagaagaaaa atgaaggaag tattatatca
     6241 aattcttgct atgcacaaga aatagggcat aaaggaggaa ctgttttgat tgaaaataat
     6301 agtgaaaaaa taaaaatctt agaaaatgat attaaacaat taataactat ttctaaggca
     6361 aataatatag atttaagtga taaaataaat tcacttaatg agaaattgga aaagcttaag
     6421 gaagatgctt tttcacatct atcagcatat gaaaaggtaa cactaagtag agatataaag
     6481 agacctacga cattggaata catagaacat atttgttcta attttttaga acttcatgga
     6541 gataggcttt ttaaagatga cccttctatg gttggaggaa ttggtcagat aggaaagttt
     6601 aatgttacta tagttggaca ccaaaaagga agagatacaa aagaaaatat aaaaagaaat
     6661 tttggaatgc ctcatccaga aggatacaga aaagcattaa gacttatgaa acaagctgaa
     6721 aaatttaata gacctataat aacgtttatt gatacatcag gagctttttg tggtctagaa
     6781 gcagaagaaa gaggacaagg agaagctatt gcaagaaact tacttgaaat gagtaaactt
     6841 tctgttccag taatcacttt tgtaattggt gaaggtggta gtggaggagc tttaggtatc
     6901 ggagtaggaa atgatgtatg tatgttagaa cattcagttt attctgtaat ttctccagaa
     6961 gggctttcta gtattctatt taaagattca tctaaagcta aagaagcttg tgatgttatg
     7021 aagcttacaa gtaatgattt atatgattta aaaataatag ataagattat aaaagaacct
     7081 cttggaggag cacaaaaaga tgtagaagct gtttcaaaag aaataaaagc ttatatttta
     7141 gaaagattaa atcattacaa agatatggat aaagaggaaa ttatagctca aagatataaa
     7201 aaatttagaa atattggaaa atgcttatag aatataaaga aatttgtagt tttttcataa
     7261 taaaatggtt aaaaaaaata aaggttttct taaaacgatt atagaagtat atttttataa
     7321 aagtatactt taaatgatga ttgtaattag taaatggcaa ttatgattta gtactttagg
     7381 taatggatac tttaacaaat taacaaggag atgttgaaat ggaggtacta aaagtttcat
     7441 caaaatctaa tcctaattct gtagctggag ccttggctgg agtactaaga gaacgaggcg
     7501 ttgcagaaat acaagctata ggagctggag ctctaaatca agcgataaaa tcaattgcaa
     7561 tagcaagagg ttttgttgca cctagtggaa tggatttagt ttgcattccc gcatttactg
     7621 atatagaaat cgaaggtgat aagaaaacag ccattaaact tataattgaa cctagataat
     7681 atatctcttt attagaattt aattaagttt taaaggttgg ctatagaaat aggacactat
     7741 agtgaacctt ttttacttta tatgttttat tttatgtaac ttttaatgta taatcttaag
     7801 taaacaaata agattggagg aaatatacat tatgaaatta aaattattgg tagaagagta
     7861 tgcagtatgt agattaaata atgacagtaa aataccaaca tggatagata cagagaagtt
     7921 ttactctata actagaactg atgatgaatt aagtgttgta tgtttaaata ataatattcc
     7981 ttctgatgtc aagtcagaaa aggagtggag aatacttaag atactaggtc ctttagactt
     8041 ttcacttgta ggaattttat ctaagttaag tagtttgtta gcagacaaca aaataagcat
     8101 atttgcaatt tctacgtatg atacagacta tatcttagta aaagaaaaag atatagaaaa
     8161 tgcttgtaaa attttaagct gtaatggata tgaagtagag tagttaaata cttttacatg
     8221 aggacttaac ttatgattta tataatcgaa attttaaaaa taagtgtaat aaatattttt
     8281 tgtttcatag gaatatttat tgtatttgga ttgattttta gcattacaga aaatctaaat
     8341 aataaattta tattttcatc ttttggaaaa actggaatta ttgtaactgg agctatagga
     8401 acatttgttc atgaattgag tcatttgata atgtgtttaa ttttcatgca taaaataaat
     8461 tcagttaagt ttttcagacc aatagaaagt aaaaatgatg gtatactagg atatgtaagt
     8521 catagctata aaaaagataa tctttatcaa agtataggaa atttctttat aggaattgca
     8581 cctattatag gtggaacaat aatgattatt ttaattttta agatattact gccagatagt
     8641 tatatgaaag tgactcaaaa tatagattta aagt
//
LOCUS       DAELNY010000161         8520 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.163, whole genome shotgun sequence.
ACCESSION   DAELNY010000161 DAELNY010000000
VERSION     DAELNY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.163"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            166..1452
                     /locus_tag="KPE55_002986"
     CDS             166..1452
                     /locus_tag="KPE55_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0494029.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1564..2037
                     /locus_tag="KPE55_002987"
     CDS             1564..2037
                     /locus_tag="KPE55_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1696..1698,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG0494030.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2069..3379
                     /gene="grdB"
                     /locus_tag="KPE55_002988"
     CDS             2069..3379
                     /gene="grdB"
                     /locus_tag="KPE55_002988"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3116..3118,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0494031.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3635..5167
                     /locus_tag="KPE55_002989"
     CDS             3635..5167
                     /locus_tag="KPE55_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG0494032.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5178..6323
                     /locus_tag="KPE55_002990"
     CDS             5178..6323
                     /locus_tag="KPE55_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG0494033.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6633..7712
                     /locus_tag="KPE55_002991"
     CDS             6633..7712
                     /locus_tag="KPE55_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0494034.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7825..8424
                     /locus_tag="KPE55_002992"
     CDS             7825..8424
                     /locus_tag="KPE55_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494035.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
BASE COUNT         3306 a          975 c         1702 g         2537 t
ORIGIN      
        1 taaaataatt tttttacaat aaatattttt tagtaataaa ataaagattt aaggcataaa
       61 aacacaataa tatatcttta aaaaaataag ataaattgta aaatgatata attttgagaa
      121 aaactcaaat gcatatacac aaattaaata acaggaggtg caagtatgcg tcttgaatta
      181 gggaaaatct ttataaaaga tattcaattt ggtgatgtaa cagaagtaaa agatggagta
      241 ttattcatca ataagcaaga gatgctacaa gaaattggtg gagatgagca tattaaatct
      301 atagatatag aattagctcg tccaggtgaa agcgtaagaa taacacctgt aaaagatgtt
      361 attgagccaa gagtaaaagt tgaaggtaat gggggaatct tccctggtat catgtctaaa
      421 gttgacactg taggagaagg aaaaactcat gctttaaaag gtgtagctgt tgtaactact
      481 ggaaaaatag taggtttcca agaaggtata atagacatga ctggagaagg agctaagtac
      541 actccatttt ctaaattaaa taatgtagta gtaatagctg aacctattga tggtttaaaa
      601 caatacgctc atgaaaaagc tgttagaatg ataggattca aagcagcaat gtacttagga
      661 gaagtagcaa gaaatcttac tccagatgaa gtttcagtat atgaaactaa acctcttctt
      721 gaatcaataa aagaatatcc agagcttcca aaagtaggat atgtttacat gcttcagact
      781 caaggtttat tacatgatac ttatgtatat ggagtagatg caaaacaaat agtaccaact
      841 ttattatatc caacagaatt aatggacgga gcaattgtaa gtggtaactg tgtttctgca
      901 tgtgacaaaa acccaagtta tgttcatata aacaatgggg ttgttgaaga cttatatgca
      961 agacatggta aagatataaa ctttgtaggt gtaattataa ctaatgaaaa tgtttatcta
     1021 gcagataaag aaagatcttc aaactggact tctaaattat gtaaatattt aggattagat
     1081 gcagttatag tttcacaaga aggttttgga aatcctgata ctgaccttat aatgaactgt
     1141 aagaagatag aaatgcaagg tgttaaaaca gttatagtta cagatgaata tgctggacgt
     1201 gatggaggtt ctcaatcact tgcagatgca gatgtaagag ctgatgctgt tgtaacaggt
     1261 ggaaatgcca accaagttgt agttcttcct aagttagata aagttattgg acatttagaa
     1321 gtagtagatg taatagctgg tggttctgat ggaagtttaa gagctgatgg aactattgaa
     1381 gttgaaatcc aagctataac aggtgcaact aatgaaacag gatttggtca tttaactgct
     1441 aaaggatatt aagaagtatt ttattaaacg attgagtaat gttaataaaa catattatag
     1501 ataattataa taagtgttat agatatataa aaatatatta gaaatataat aaggaggatt
     1561 attatgagtt tacttagtaa taaaaaggtt cttataatag gtgaccgtga tggtatacca
     1621 ggacctgcga tagaagaatg tgtaaaaaca gtagaaggag cagaggttgt tttctcatct
     1681 acagaatgct ttgtctgaac agctgctggg gctatggact tagaaaatca aaacagagtt
     1741 aaagatgctg ctgataaatt cggagctgaa aatgttgtga ttttactagg tgctgctgaa
     1801 gccgaagctg caggtcttgc agccgaaaca gtaactgctg gagatccaac tttcgctgga
     1861 ccacttgctg gagttgcctt aggattaagt gtttaccacg ttgttgagga accaataaaa
     1921 tcattatttg atgaaagtgt atatgaggac caaataagta tgatggaaat ggttttagaa
     1981 gttgaagaaa tagaagaaga aatgtctggt ataagagaag aattttgtaa attttaatgc
     2041 ctaaaagcaa aaggggtgaa actaagagat gggaaaacta aaagcagtac attatattaa
     2101 tcagttcttt gctggtatag gtggagaaga aaaagctgat actaagccac atgtggcaga
     2161 aactttacct ccaataagtt tacaacttga taaattatta ggtgaagata ttgaaatagt
     2221 tggaacagta gtttgtggag atagctattt caatgaaaat atagatagtg caagtgaaga
     2281 agttttatca atggtaaaag gttttgaacc acaacttttc atagctggtc ctgcatttaa
     2341 tgctggtaga tatggggttg ctgctggaac aataactaaa gttgtaaaag atgctttgaa
     2401 tataccagct ttaacaggta tgtatgttga aaatccagga gcagatatgt tcaaaaagga
     2461 tgtttatgta gtagaaactt ctgattctgc agcaggtatg agaaaggcac ttcctaagat
     2521 agctaaactt gcagtaaaac ttgctaatgg agaagaaatt ggaactccta aagatgaagg
     2581 atacatagct agaggaataa gagttaacta cttccatgaa gacagaggtt ctaagagagc
     2641 tgttgacatg ttagttaaga aaataaaagg tgaaccattt gaaactgaat acccaatgcc
     2701 aaactttgat agagttgacc caagtaaagc tgtaaaagat ttatcaaaat gtaaaatagc
     2761 tttagttact tctggtggta tagttccaaa aggaaatcca gatagaatag agtcttcttc
     2821 agcttctaaa tatggaactt attctatagc aggagttatg gatttaacag aagagactta
     2881 tgaaactgca catggtggat atgacccagt gtatgcaaat ttagatgcag atagagtatt
     2941 acctgttgat gttcttagag atttagaaaa agaaggtgta attggaaaat tacatgagac
     3001 tttctataca actgtaggta atggtacatc tgttgcaaac tctaaaaaat atgcttctga
     3061 aattggagca gcattagtgg ctgatggtgt agatgcagta atattaactt ctacatgagg
     3121 tacttgtact cgttgcggtg caacgatggt aaaagaaata gaaaaaactg gacttccagt
     3181 agttcatatg tgtacagtag tacctatttc attaacagta ggggctaata gaatagttcc
     3241 aactatagca atacctcatc cacttggaaa tccagcgctt gaccctacag aagaaaaagc
     3301 cctaagaaga ggccttgtag aaaaagcatt aaatgcttta actacagaag tagatggtca
     3361 aacagtattt gaaaaataaa aataacacac acatatatta tatagataaa aatataaaca
     3421 cacactaaaa ataaaaaata actatacaca aaaaaataat atagatttca tgaatggata
     3481 aattggagtt attcattcat gaaatcttaa aaaatgccaa aatacataaa caataataga
     3541 atcaaatata caataaaata caatgattag agcttttaat aagtttaata agattaaaaa
     3601 ataagtgttc ataaaattta tggaggtgca caaaatgact tatccagttt taaaaggggc
     3661 aggctatgta cttattcata ctccagatat gatagtgcaa aatggaagta cttgtacagt
     3721 tgaaagagca acaaatccag attcagaatt tttaaaagaa gtaagtaatc atataagaag
     3781 ttatgaagat gtagttaact acatgccaaa tcaagtttat ataggaaata gaagaccaga
     3841 agaattaaga gatttaccaa tgccatggtg tgaacaaaag attgagggaa ctagaaatgg
     3901 taagtttgga gaaataatgc ctcaagatga gttcatagct ttaatgcaaa taagtgatgc
     3961 atttgattta gtaaaattat cacaagaatt tattgatgaa gtaaagccaa aaatcgaaaa
     4021 taactaccct gaaattgcac catttgtagg taaattaaag ggtgatgata tagaagaagg
     4081 taaagaatta gtagcaacac acatagctga aggattatac catgatggaa aatttgttgg
     4141 ttatgtaaaa agagcacatg atgtagatgt taacttaaat gcccatacaa tgtttgaaaa
     4201 tctagtagta aaagcatctg gagttttatc tgctattcaa atgctaagac atagcaaaat
     4261 agaccctgct gaaatagatt atgttattga gtgttcagaa gaagcttgtg gagatataaa
     4321 ccaaagaggt ggaggaaact ttgctaaatc aatagctgag atagcaggac ttcaaaatgc
     4381 aactggttct gacacaagag gattttgtgc agcaccaact catgcattaa ttcaagcagc
     4441 agctttagta aaagctggca tacataaaaa tgtaatggta gtagcaggtg gagcaagtgc
     4501 taaacttgga atgaatgcta aagaccatgt taaaaaaggt cttcctgtac ttgaagatgt
     4561 tgttggagga tttgcagtac ttgtatctga aaatgatggt gtaaatccag ttataagaac
     4621 tgatttaaca ggtaagcata ctgttggaac tggttcttca ccacaagcag ttatgacagc
     4681 acttataact tcaggtcttg atagagctaa tttaaaaata acagatgttg atgtgtattc
     4741 agttgaaatg caaaacccag atataactaa gccagcagga gcaggagatg ttccagaagc
     4801 aaactacaaa atgatagggg cacttgctgt taaacgtgga gacttagaaa agaaagaatt
     4861 gaaagatttt gtttctaaca aaggattacc aggatgggca ccaactcaag gacatatacc
     4921 atcaggagct ccttatatag gatttttaat agatgattta actacaggaa atagaaatag
     4981 agctatgata gttggaaaag gtagtttatt cttaggaaga atgactaact tatttgatgg
     5041 agtatctttt atagctgaaa gaaatacagg tgttactgaa gagacttcag gaatatctaa
     5101 ggatgaaatt aagaagataa tagctgaatc tatgaaaaaa ctagcattag atatgttaga
     5161 agaatagggg tgaggatatg tcaaaaaaag taatcgcaga tgtattctta gaagtagcta
     5221 atgcaataga aagcggcgaa tttggcaaaa aagtaaaaat tggtgttact acacttggaa
     5281 gtgaacatgg tgtagaaaac atggttaatg gtgcacaatt agcaaaatct aatttatttg
     5341 atattgtgct tattggacca aaagtggaaa ctgaccttga agtagttgaa gttaatgatg
     5401 aaaaagaaat gcatgcaaaa atggaagaat tattagattc aggatatatt gatgcttgtg
     5461 ttactatgca ttataatttt ccaataggtg tatctacagt aggtagagta ataactccag
     5521 caaaaggaaa agaaatgata cttgcaacaa caacaggaac aagtgctact aatagaatag
     5581 aggcaatggt aagaaatgcc atttatggta ttgctacagc taaatcaatg ggtaacaaat
     5641 gcccaaaagt tggaatactt aatgttgatg gagcaagaca agttgagaag tgcttaaaag
     5701 aattaaaaga taatggatat gacatggagt ttgcggattc aattagagca gatggtggat
     5761 gtgtaatgag aggtaatgat ttacttgtag gtgcacctga tgttatggtt actgatacat
     5821 tatctggaaa tatatttatg aaagtatttt cttcatatac tacaggtgga gattatgaag
     5881 ctcaaggctt tggatacgga cctggagttg gagaggacta tgatagaaag gtacttatag
     5941 tatcaagagc atcaggttct ccggtagtag caaatgcttt aaaatacgct tatgatgttg
     6001 taaagggaga tataagtaat gttgcaagaa atgaatttgc aaaggttaaa aaagcaaaat
     6061 ttgatgacat tatatcctct cttactaaaa aagaagttaa agctgaaaaa gtagaagtta
     6121 aaatgccaga taaagaaatt gttactcgtc aaatagctgg tgttgatata atggatttag
     6181 aagatgcagt atctgagtta tggaaaaacg gaatctatgc tgaaagtggt atgggttgta
     6241 caggtccaat agttttagta aatgaagcta aaggagactt agcagttgaa actttagtta
     6301 aagctggata tactgcaaaa taagtacatt ttttatatgt aaataagaac atatagttat
     6361 aattttattg attataatta tatgttcttt ttattttata tcttgtcaat atttatatat
     6421 taacacaatc catttatttg attctctagc taatttatta tatttattca gttaaaatta
     6481 agctttctaa ctaaataaaa actaatgaag tattaaaatg aaattgataa aatatgaaaa
     6541 aattttagga ataaagtatc tgttagataa aaataagatt aattagtgat ataatataat
     6601 taagataaaa tttattattt ggaggcaaaa tcatgagcag agtaaaaaat gtagtagaat
     6661 tgttggaaac taaaggtgtt gatgcactat atctaacaaa gaaaactaat gtaaactata
     6721 taagtggttt tcctgatgaa gaagcatatg cagtgatttg caaagatgga aactttttag
     6781 ttactgatag tagatatatg gagcttgcag aaaaggtttg caaagatttt gaaattataa
     6841 attggcataa ttttgataga agtgtggcaa aggcagttaa aagtgtatgt gataaagtgg
     6901 gtataaagaa acttgggttt gaaagaacta atatagtgtt tgataaatat gaagaactta
     6961 aaaatctgat tgagaaggat aatggagaat taatacctac agaaaatatt gtagagactt
     7021 tgagatatgt aaaagacaaa gatgaaatca aaaatacaag aaaagcttgt gaaatagcgg
     7081 ataaggcatt agaagaattg attccacata taaaagctgg agttagcgaa attgaactgg
     7141 ctacaaagtt agagtacttt atgaaaatga acggagcaca aaatataggg tttgaaacta
     7201 tactgatatc aggagcaaag acttcattgc ttcatggtaa gcctagtgat aagattatag
     7261 aaaaaggtga ttttgtactt atagactatg gcgctatgta taatggctat atatctgata
     7321 caactagaac ttttatagtt ggaggagctt ctgaaaagca attagaaata tataatttag
     7381 ttaaggaagc tcaaaatgta ggggttgaga acatgaaagc tggtgtacat gcgacaattc
     7441 cagatgctga gataagaaaa gttgtcaaaa aatatgaaga ctattactat caaggtatag
     7501 gacatggagt cggtagagat gttcatgaag aacctttcat tggtaattat ggtgataaaa
     7561 tcatagaaga gggatgtata ataacaatgg aaccaggtat ttatttccct ggatggggtg
     7621 gagttagaat agaagatact gttttgatta ctaaaaatgg tccagaaaga cttactaaat
     7681 tccctaaaga tttgatgatt ttagacaaat aatttaattt atatatattt ttttacataa
     7741 aatataacat aatctcacta ttaagctcta tttacatatt atagtaaaga gggcttaatt
     7801 ttattgtgag aggtgatagt atatatgtat aaaaaacata aatttataaa aaatataaaa
     7861 aaagcctggt ataaaatcaa attaattgat agatgcctta taattgtaat ggcaatatta
     7921 atgtttcaat ctatatataa tttatttgtt aatgaagtaa attcacaaga tactacaact
     7981 attgatgtag tagtaagaac cacttcagca gctatatttg ggtatttttt aagtgctaat
     8041 tttataaaga gacctttaag gaagtataca aatactgatg tatcttcaaa tctttttata
     8101 gataataatt tatccccaaa aggtcaagag tctaatcaaa ataatataat gaatgtcaaa
     8161 aataccattg gatttacttc agaagctgat aattatgaaa agaaaatacc tattaataat
     8221 aatgaaggtt ttgaagaagg agaaactagt gaattgcaaa ttattatagc tactgtaatt
     8281 tgtatagtag cattaacagt actttttgtt gtaagaaatt ttacaactac aacatcagct
     8341 tctttaggta ctatttcaca gatgagagat tttgtctcag gatgcgtggg ttttttgcta
     8401 ggttgtccaa gtaaacctga ttaaaaaagt attatcaata aaaggtattt taaaaagtat
     8461 cagtataata atatttagat ttaaatgtat acaattataa ttctaaacat tctatattgt
//
LOCUS       DAELNY010000162         8454 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.164, whole genome shotgun sequence.
ACCESSION   DAELNY010000162 DAELNY010000000
VERSION     DAELNY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.164"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..357
                     /locus_tag="KPE55_002993"
     CDS             <1..357
                     /locus_tag="KPE55_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0494036.1"
                     /translation="IENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSH
                     IYETKCKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMR
                     SKDIKKTISNYNEKLN"
     gene            545..1939
                     /gene="yfcC"
                     /locus_tag="KPE55_002994"
     CDS             545..1939
                     /gene="yfcC"
                     /locus_tag="KPE55_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG0494037.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            2456..3823
                     /locus_tag="KPE55_002995"
     CDS             2456..3823
                     /locus_tag="KPE55_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG0494038.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            3848..4456
                     /locus_tag="KPE55_002996"
     CDS             3848..4456
                     /locus_tag="KPE55_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG0494039.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            4504..4875
                     /locus_tag="KPE55_002997"
     CDS             4504..4875
                     /locus_tag="KPE55_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG0494040.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            5054..5392
                     /locus_tag="KPE55_002998"
     CDS             5054..5392
                     /locus_tag="KPE55_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG0494041.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            5858..6361
                     /locus_tag="KPE55_002999"
     CDS             5858..6361
                     /locus_tag="KPE55_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG0494042.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            6408..7367
                     /gene="moaA"
                     /locus_tag="KPE55_003000"
     CDS             6408..7367
                     /gene="moaA"
                     /locus_tag="KPE55_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG0494043.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            7371..7841
                     /gene="moaC"
                     /locus_tag="KPE55_003001"
     CDS             7371..7841
                     /gene="moaC"
                     /locus_tag="KPE55_003001"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG0494044.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            7877..8308
                     /locus_tag="KPE55_003002"
     CDS             7877..8308
                     /locus_tag="KPE55_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG0494045.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         3430 a          756 c         1505 g         2763 t
ORIGIN      
        1 attgaaaata agtataattt taatgaaaat aaaatactag gattatcttt acataaagta
       61 gaggaagtag attatttaga aaaattaata agaaatcaaa atataaaaat agattacata
      121 acattatcac atatatatga aactaaatgt aaagaaggat taaatccaaa aggaattgag
      181 cttttaaaag aagctaaaaa gattactgat attaaaataa ttgcacttgg gggaatttta
      241 ccatctaatg tcaaggaaac attaaaatac tgtgatgatt ttgctataat gtcaactata
      301 atgaggtcta aagatattaa aaaaaccata tcaaattata atgaaaagtt gaattagtaa
      361 atatagtata ttgaattttt aacttaaata aaaataaaaa agttatcttt taaattttaa
      421 aaggtagctt ttttatttta tatctttgct aataaaaaat tgtaaaaagg gaaaacttta
      481 gtcagagaga ttaaacaata tcctagaaag atttaaaatt tagtaaagta tggaggacat
      541 tataatgact gacagtaaaa agaaacgatt tattatacca cacactttta caataatatt
      601 tttactaata gtcttgatgg caatattgac ttggatagta ccaagtggtg aatttagtag
      661 agaagatgta gaaggtagaa tggtagttgt accagggact tatcaaagtg tagaaagtaa
      721 tcctcaagga tttgcagatg tttttaaagc accaatagaa ggctttatcg attcagctga
      781 agttgtaggt tttgttttaa tagttggagg tgcttttggt attgtaaata ggactggagc
      841 tatagaagca ggaatagctt cggttatctc taagtttaag ggaaaagaac ttcttataat
      901 tccaatatca atgattttat ttggacttgg aggtacaaca tttgggatgg cagaagagac
      961 attaccattt tatatgatat ttgttccact catgacatcc ttgggatatg attctctaac
     1021 agcaatagca attgtattta taggagcagc agcaggagca gccgcttcaa caataaatcc
     1081 attttcagtt ggtattgcac aggcactagc tcaacttgct cctggttcag gagtaactta
     1141 cagagtagtt atttttatag catatatact aataagtata gcatttgtta tgtactatgc
     1201 taagaaggta aaggaaaatc ctgaaagttc aatagtatat gaaatagata aaaataataa
     1261 atcaatactt aatcaaactt caacagaaat aaaagaattt acttttaaag aagtatctgt
     1321 tcttgctata tttggaatag ggatggcaat aatgatttgg ggtgttttaa gtcaaggatg
     1381 gtacattcca gaaatatcaa tgtgttttgc tgcaataggt gtgctttctg gattggttgg
     1441 tagattatct caaattgaaa tctcagaaag ttttatagat ggagcaaaag atttagcatc
     1501 tgcagctctt gctatagctt tggctcgtgg aatcgtaata attgctcaaa atggatttat
     1561 aatagatact atattaaatt ccgcagcagc ctttctaggt gggcttccaa aggttatatt
     1621 tgtatattta tcatttttct tggaagctgc attggcattt ttaattccat catcttcagg
     1681 tcttgcttca cttacagtac ctgtacttgc tcctcttggt gatttggtta atatatcggc
     1741 tcaagtgata gtaaccgctt atcaatttgg tgtgggtctt acaaacttta taactccaac
     1801 ttcaggagta ctgatgggag cacttgcagt agcccatatt ccattttcaa aatgggtaag
     1861 atttataatg cctatcttgt tgattttgac agctgtatct ctagtctttt taacaatagg
     1921 tatttatata gggttttaat taagtgtagt tttatttaag atatttagta atggtataaa
     1981 atgataatat gttttcaata aattgccact aatttaatta gtggtaattt gttttttgta
     2041 tttaaaaata aatgtattta cttgtatttt gaattaatat ctacttagac attgttttaa
     2101 ttgttatatt tttatttaat taagcattga tttatcattt tttatatttc atataacaat
     2161 gcaaaaaata atttttatat agtaaatgac aaaagtttac aataaatgta tattttctta
     2221 ctcattcctt aaaattactc atttagttta ataactctca tttcatatgt aaagttggta
     2281 aaaaaattaa aattaaaagt aaaaaactaa ataaattaat gttaatttga aatatatatg
     2341 aaggtttttg gagaatactt acttatgata ggaaaaaatg ttaaaataat agtgtaatca
     2401 aatataaagg actgaaataa ttaacagtta ttaaatatta ggagtgtaaa aatttatggg
     2461 attaaaagaa tttgatttta ttgaagagtc tattgatatg ttgaggacaa tgtcaccaac
     2521 attagaaact atatcagatg aaatagaaga atattttgaa aatatcttgg atgaaaagaa
     2581 tcaagaatat ataaatgtaa cctctagaat aaaatcagaa tcaagtttaa gagaaaagat
     2641 aattagaaat agatatttaa aaaaatatgg agaagcaagt aatcttattc ataatgtttc
     2701 tgacttaata ggcctaagaa ttgagtgtag atttattgag gatgaaaaca aaatatatag
     2761 acttttgaga agatatttta ataaaacaga tgacaaaata aactattata ataaagagaa
     2821 taagaatatt aaattaaaac tatctgagag gcaacccaat aagcaaaaaa atggatttga
     2881 aatatataaa atagatggag tttttgcgta tttagataga gaagtaaaat ttgaacttca
     2941 aattaaatct cttgtaaatg tgttttggag tgaaatagaa cataaaataa tttataaaaa
     3001 caatacatat ttacttgctg ataaatttat aaaggatatg atggactcta taaagaataa
     3061 tcttactatg atagataatc agcttttaag tatttataaa aactttcatt cagggaagtc
     3121 ttttaatatg aaagtaagta aaaaagaaat tgaaaaatta tttgcgaaac tagtctatga
     3181 cgctttttct gaaaagatga ataaaagtat aggttttgtt gtagatttta aaaaaccatg
     3241 tgagactata ttgaactatt cttttaataa acaagatata agtgatgaag ttttaggtaa
     3301 ttttatgctt gatgagtttg caagattaaa tgaaattgta aacaaggata tagattttaa
     3361 tgagcaaata gaatttgaaa gagaaccaac atttgatgat aagttttgta aagacctagg
     3421 aaatcatttt agaagtagat taaacacaga atttccatgg aacctatttt ttagaatatt
     3481 atttgaaata gagccatata acaataccaa agactttgaa aactttgtag agtttataaa
     3541 agaaaatgtg ctactagaag aaaataggga gcaattatta tgccaatttg aagaggatag
     3601 taagcttata attgaagata tatatgattg tatatttaat agtataagtg agattgatag
     3661 tgttgaaatt ttatataact ataatcttga aaaaataaac tttacatcaa gtgaaatcat
     3721 aaattgtata tgtagagaat atgagtgtta tggtgaatac ttagaagaaa aagaaaagct
     3781 tatgaataca tttaaagaaa agattattca aatctttgag taataggtca atgctgaagt
     3841 ggcgctaatg cgccattttt caaatatata tgaattaagt aaaattattt tggaggaggc
     3901 gatttgtata gagaaaacag gtgtaatttt ggcaggggga aaaaattcta gaatgggtag
     3961 agataaagcc tttttagagt tacatgataa gctttttatt gaaattgcaa tagaagcatt
     4021 taaaaacttt gatgagttga taataatatc taacaatgaa gaattatatt ctaaatacga
     4081 tattaaagtt tataatgata tagtaaaaga tgttggtcca attggaggaa tatatactgc
     4141 tcttgaatat gcaaagtatg atatagttac aattgcatgt gatatgccat atctaaataa
     4201 tcaaatagtc gaaagaatag ctaataaaat ggatgataaa agtgtgattt cagtgacaaa
     4261 tggaaagtta caaccattgt gttctggata taaaaagagt ataataaata aagtatcttt
     4321 atgtataagg gaaaatgatt taaagctcag aagctttata gataaaatag ataaaagtta
     4381 tatatacttt gatgaagaag atttattttt aaatgtaaac actgttgatg agtataaaaa
     4441 attaactaaa gtttaattag ctatggttgt aaaatagagt aggatttata tggaggagag
     4501 tttatgaatt tacttaaatg cgattcttgg gcagtaatat taaataatag cgataaagaa
     4561 agtaaagcat ataaaatatt agatgaactt aaaaggaata tgtacaaagt tgttgctata
     4621 gatgaagaga aaaaacctat tgaaggaata gatgtgtatg aatgtttaaa agatgttcca
     4681 cacaatattg atgttgtagc tattattgat aaacaatcta agatggatgt gattttagaa
     4741 gaagtagagc ttttggatat acaaaatatg tggtttgaaa aaggaagctt tagtgaaatg
     4801 atgattaaaa agactaaaga tttaaaatta aatatagaat ataatttaag tttatatgat
     4861 gagttgacta gataaaatta tatttcttaa taataaaaat ataattagtt aataaaacat
     4921 attattttat agtatagatt ggttaataaa agtacagatt ggttaatgaa acattaaatt
     4981 acaatatttg ttaaaaaaat tgtgagttat atgatataat gtattaaata ggatactaag
     5041 gagttgatat attatgttaa tagtaactac agaaaaagta gaaggtaaaa agatatcaaa
     5101 ggttttagga ttagtgagag gaagtacaat aagagcaaaa catgttggaa aagatatagg
     5161 agcaagtttt aagaatcttg ttggaggaga acttactgga tataatgaaa tgctcactga
     5221 agcaagacaa attgccatag gtagaatggt tgaagatgca gaagctaaag gtgcaaacgc
     5281 agtaatagca tttagactgt cttcagcttc agttatgcaa ggagcagcag aaatgcttgc
     5341 ttatggaaca gcagttgttt tagaagatga taatagtatt cttgaaaaat aaaatataaa
     5401 tagttaaaaa gaggcatctt ttaaaaattt tatggatgcc tttttttatt tgaaaaaact
     5461 aatttgagaa gaaattttaa ctattatgac aataaaatta gggtgatatt atataaataa
     5521 gtaaaattat attatataat gtatggtata gactttaaaa attttatttg tagagaaaaa
     5581 tatggacaat gttataataa ttatagaaat taaaagtact tacattcatt atgtatgtga
     5641 catgtataat tagttttata tttaatacag ttttgaagta atatcttcat tttgagaacg
     5701 ttggaataat aaaaaatgta ttattatagg tgatataaat aaaaaataaa tataatatgt
     5761 tatattgctg atagaaaaga gaataaaaga aaataggttt tattaaaaga gatttttatt
     5821 tataggactt aatcaatgta tagaggggtg agaacatatg tttaatgttg caattataac
     5881 acttagtgat aaaggatatg aaggaaaaag agaagatata acaggaaaaa agttaactga
     5941 atttgttgaa aatacaggag tttataaagt tactgaatat gtacttataa aagatgataa
     6001 ggaaatgcta aaggaaaata ttataagact ttgtaatagt aataaaatag atttaattct
     6061 tacaaatgga ggtactggtt tttcaaaaag agatataacc ccagaagcaa ctaaggaagt
     6121 gttggaaaaa gaaataccag gtctgagcga atatatgaga atgaaatcca cagaaattac
     6181 taaaaaggcc atactaagta ggggtgtgag tggaataaga aataattcta taataataaa
     6241 tttacctgga agtcctaaag gtgcagttga aaatctaagt tttataattg atgttttaga
     6301 ccatggcata gaagttttaa gaggagaagc tacagaatgt gccactaaaa aaagtgaata
     6361 gtatataatc tagatagata aaaaatagac aaagaggtag ataaagtatg attgataagt
     6421 atggaagaaa aatagactac cttcgtattt cacttacaga taagtgtaat ttaaggtgtg
     6481 tctattgtat gccaccagat gtaaaatttg ataaaaatta tataaatgaa aatctaagct
     6541 ttgaagacta caagtttatt ataaaggcta tggctgaaca aggtataaca aaaattagat
     6601 ttactggagg agaacctctt ttatatccta aattaaatga gcttataaaa tttacaaaag
     6661 aagaatgtgg aataaataac ataggaatga ccactaatgc tattggtctt tctgatagaa
     6721 tttttgagct tgaaaaatgt ggcttaaata aagttaatat aagtttagat tctttgaagg
     6781 aatataagta taaatccata acaagaggag gaaacttaaa agatgtctta aaatcgattg
     6841 aatcttgttt aaatttaggt ataaaagtaa agttaaattg cgttgctata agtgggttta
     6901 atgatgatga gcttaaagat tttatgtttt taacagttaa atcaccaatt gatgtaagat
     6961 ttatagagct tatgccatta ggtgaggcta atagagttta taagaagggt tattttaatg
     7021 taaaagaaat gattgaaaaa atatatggtt tatataaagt caaaaatgat gaaaaaagta
     7081 cagcagaata ttatatgttt aaaggagcta aaggaagaat aggaattata actccattaa
     7141 gctgttcatt ttgtaacact tgtaatcgta ttagacttac ttctagtggt gcaataaaat
     7201 tatgtctaca ttcaaaagaa gaaatagata taaaaccatt tttacataaa ccattgttat
     7261 ttagagaatc tatgaaagat ataataaaac aaaaaccaga aaaacatcat ttaatagaaa
     7321 ataaatgtag tgatacaaat aggcgtatgt atgaaattgg aggataagta atgttaacac
     7381 atataaatga aaaagggtat gccaagatgg tggacataag tgaaaaagat gatactaaaa
     7441 gaacggccgt agctacagct acaattactc ttagtaagga agcattagaa aaagtaaaaa
     7501 atggtcagat aaaaaaagga gacgtattgt ctgtagctca agttgcaggt ataatgggag
     7561 ctaaaaatac atcttcaaac ataccaatgt gccatcagat taaattagtt ggatgtgatt
     7621 tagaatttga tgtagatgat tctatgtctg ctattaagat atacgcaacc tgtaaatctc
     7681 ttgggaaaac tggagtagaa atggaagctt taaatgcatg ttctttagca gcacttacaa
     7741 tttatgatat gtgtaaagca gttgataaaa aaatggttat aagtaatatc catcttgtgg
     7801 agaagactgg tggaaaatca ggagatttta agttcgagta aattaataat atattgggtt
     7861 aaaatttgga ggaaatatgg cgaaggtagt gtcaataaat ataagtgaga aaaagggtgt
     7921 aattaaagtt cctgtacaat gtgcagagct aaaaataaat catgggatag ttggagatgc
     7981 acatgcagga aactggcatc gtcagataag cttacttgga aaagaaagca tagataaaat
     8041 gaaaatgaaa ggctttgacc aattaaaata tggagatttt gctgaaaatg taactacaga
     8101 agggatagaa gttttttccc tgcctgttgg tacaaagtta agaatagggc aatgtgaagt
     8161 tgaagtaact caaataggga aaaaatgtca caatggatgt gaaattaaga aattgacagg
     8221 tgactgtgtg atgccacgag aaggaatttt tgtaaaggtt ctaaaagaag gaattataaa
     8281 tgtgaatgat tcaatagatg taatctaatg aagtcactat taaaatataa ttataagtaa
     8341 atactattaa gatttaaata tatattttaa gagagaatta attttaataa aatattttag
     8401 ttataaattg gaatattggt tatataaagc tatgaatgta aaatcatagc tttt
//
LOCUS       DAELNY010000163         8331 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.165, whole genome shotgun sequence.
ACCESSION   DAELNY010000163 DAELNY010000000
VERSION     DAELNY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.165"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(203..727)
                     /locus_tag="KPE55_003003"
     CDS             complement(203..727)
                     /locus_tag="KPE55_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0494046.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(792..1544)
                     /locus_tag="KPE55_003004"
     CDS             complement(792..1544)
                     /locus_tag="KPE55_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0494047.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1575..2873)
                     /locus_tag="KPE55_003005"
     CDS             complement(1575..2873)
                     /locus_tag="KPE55_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG0494048.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2890..5154)
                     /locus_tag="KPE55_003006"
     CDS             complement(2890..5154)
                     /locus_tag="KPE55_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG0494049.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5409..6227)
                     /locus_tag="KPE55_003007"
     CDS             complement(5409..6227)
                     /locus_tag="KPE55_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0494050.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6220..6918)
                     /gene="cbiQ"
                     /locus_tag="KPE55_003008"
     CDS             complement(6220..6918)
                     /gene="cbiQ"
                     /locus_tag="KPE55_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG0494051.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6921..7199)
                     /locus_tag="KPE55_003009"
     CDS             complement(6921..7199)
                     /locus_tag="KPE55_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0494052.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7189..7941)
                     /locus_tag="KPE55_003010"
     CDS             complement(7189..7941)
                     /locus_tag="KPE55_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG0494053.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8123..8315)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2621 a         1516 c          897 g         3297 t
ORIGIN      
        1 taaacaatca taataaaatt tttttaataa aactttattt tttagttaaa atcatacaat
       61 atatagattt ccacaataca aataaattaa aaagtgaaat gtatttgtct aaaaacataa
      121 taacatttca ctttatcctc atttttaagt aatatatatt tttaattttt atttataact
      181 aaatctagtt tttatataaa ttttagtttt tttctacatc tgagtaatct attccaaaag
      241 tttctacagt aacagatttc ataacttgtg gagtttgtgg cttatcagct gaatctgttc
      301 taactgatgc tatttcatca actgtatcca taccttctat cactctacca aaacctgcat
      361 actgaccatc taagtgtggt gagtttttat gcattatgaa aaattgagaa ccagctgaat
      421 taggtgccat agttctagcc attgatatga ctcctctttc atgttttaaa ttgttagtaa
      481 atccattacc actaaattca ccttttattg agtgacctgg accacccatt cctgtaccat
      541 ttgggcatcc tccttgtatc ataaatcctg gtattactct atgaaatatt attccattgt
      601 aatatccttc atttactaat gatacaaagt ttttaactgt atttggagct atatttggat
      661 ataactcaat tttgatttct tttccatttt ccatttctat agttactata ggatttttat
      721 tttccataat ttattacctt ccttttcatt agttaaatta cataattaca tttattatat
      781 tatactctaa attaaagtct atcaagtata ttttttccta tttcatatcc ttcttgataa
      841 atagttttac aaaactcatc atcaaagtta agcgtgtctt taggagcaaa ttgagtatta
      901 ggtctaacta ctattatttt atcttcatca tatacttttt taatatcttc tggcaactca
      961 taattatatt ttgtggatat taaaataatt ttatccacat tatccccaat taatggttca
     1021 attaatgcac tcctaattaa acctccatct aatttgaaac catcataaat tccttcttgc
     1081 acatctttca caaattgttc tttaaatcca agagttgcgt ttggatttga tggtaacaac
     1141 ccactgtatg aaatgctgat aatggcttca ttacgaggca tttttgatac gtcaacaatc
     1201 ttttctgata ctactttatt atcaacttca acataattaa cataaaagtt catttcttct
     1261 atattactat catctagttc ccttaggttg tctatagcag gagaattatc cacagtatta
     1321 tttactattt ttacatttcc attctggata tcttttatat ttgtccacat tttctccaag
     1381 ttatcaacat tccctgtgta caaataatat ccatttattg ctcctattga tgtccctgaa
     1441 atcgaataaa atttatttat tcctccatca tacaaggctt taactactcc tgcttgataa
     1501 gccccttttg ctcctccacc ttctaaacat aatcctaatt tcatagtctc atctccaaat
     1561 tgtttatctt atatttatat ttcaaaaaac ttactaaatg ttttatatat actatagtgg
     1621 tcctcaacat ttcctaattc tcttatagaa tgcattgcta aaattggact tcccacatcc
     1681 acagatggta tatctatatg agtagaagat attggaccta tagtacttcc tcctctttca
     1741 tctgacctat taacaaattc ttgatactca acattagctt ctctacatat atttttataa
     1801 atagctatag aatatccatc actagtatat gcctgactag catttatctt tataaccgga
     1861 ccttttccca ttattggttt attagttggg tcatgctttt ctggaatgtt tggatgaata
     1921 gcatgagcta aatctgatga tatcataaaa gatgaataaa tagactcaaa gaatccctct
     1981 ctatccttac ccattgatat gcaaattctc tccaatatat ttaacaacat gtttgaatct
     2041 gcaccttgtt tagtagagct accaacttct tcattgtcaa atacagccat tacatttaca
     2101 ccatgttgac ctttagtatt aacaagagca ttcaaactag catgtaccat tgcaagatta
     2161 tcttgtctcc caatagatat aaattcatca ttagccccta ttaaacttcc tttttcaaat
     2221 tcatataaaa ataaatcaaa atctataata tcttccatat ttacatctaa gttagatgct
     2281 atttgcttta taagaaaatt atctttttct aatgtatcat ttaacatccc aactaaaggt
     2341 aacatatctt tttgcttatt atatgaatga ccatcattta tacttctatt tagatgtatt
     2401 gctagatttg gtattataca aattggcttt ttaaagtcta taagcctttc atttggtttt
     2461 aatatactat caccttttaa aactactcta ccagctatcc caagaggtct atccaaccat
     2521 gtacttaata ttgctcctcc atagcattct gtatttaact tcaaataaga tttttcagct
     2581 tctatttctg catttggttt aatactaaat gtaggagaat ctgaatgaga gcctattatt
     2641 ctaaatccat ctctttctat accttctgaa tttactacaa aagctactat agctgataaa
     2701 ttcttagtaa cataatactt tccaccaact cttagattcc atctttgatt caaaactaat
     2761 tcttcaaatc catttttatc taagatttcc ttagtactct cgactgcatg aaaagacgac
     2821 ggactatcat atataaagtc tattaagttt ttagcaaagt ttatactatc cattcctcat
     2881 ttacctccat tataaaatat ttagtgttgt atattcttta tttatttttt taattccctg
     2941 ttggtcaaaa gctatagtaa catctgtacc aattatagaa accactgtcc caaccccaaa
     3001 tttaggatga tgtaccttag aaccaagttt tatatcatct ggattagttt ctttattaga
     3061 atcttttatt gtagcattaa ctttatctgc aactttagat ttgcttatag tactcatata
     3121 ctttttagta tatttatcta acatattata acttgcttta gaatagctta gttctttttt
     3181 tactttgtac aatctttcta tacattcttc tggcagttct tccataaatc ttgaagctat
     3241 cgcaacattt gttttaccat ataatgttct cttctctgtc aatgacatat atagctcttc
     3301 tttagctcta gttataccaa cataacaaag tcttctctct tcttctattt gtgaatcact
     3361 catagatttt atagctcttg atataggaaa taacccttct tccatacctg ttaaaaacac
     3421 aacagggaac tctaaacctt tggatgtatg aattgtcata agcgaaaccc tatcattgtc
     3481 ttcttcatca tttgattcag atgtaagtgc tatacttgtt aggaatgtct ctaagtcttt
     3541 attttcataa gtatcatcat tactactttc aaactccaaa gctatagaaa taaattctct
     3601 caaattatca attctgtctt gagcttcttc acctttgcct gttaaatctt tttcattttt
     3661 atttcttatc tctacaagct cgtccatata tcctgtagta tccaagactt tttctataag
     3721 tttgctaaca ggataaactt cttttatagt tctaagagtc cctataatat caacaaatcc
     3781 acttatactt gcccttgcct tagtagatat atctgaattt gtttctatat caattaatac
     3841 tgagtatata ctttcctgtt ttaagttagc tctatcttca attttttcta tagttctaag
     3901 ccctatacct cttctaggaa catttataat cctttttata gatatatcat cttgtggatt
     3961 ttgaataact cttaaatacg ccaacaaatc ctttatttct tttctctcat agaattttgt
     4021 accaccatat atattgtatg gaatttgact tctatttaaa gcgtcctcta caggtcttgc
     4081 ctgtgcattt gctctataaa gtactgcaaa atccttataa ggtctatttt cttttctagc
     4141 tatttgtgca attgaatcag caatgaaatc agcctcttct atttcgctac cagttaattg
     4201 tatctttata agttctcctt cttttttttc actccaaagc ttttttcttt ttctttctat
     4261 attattagat ataactttat tagctgcatc taatatcact tgtgtagacc tataattttg
     4321 ttctaacttt acaacatgta cattgtcata atctttctca aattcaagta tgtttcttat
     4381 atctgcacct ctccatccat aaatactttg gtcatcatct ccaacaacac aaatattttg
     4441 atgttctctt gctaataatt ttatcaattc atattgagct ttacttgtat cttgatactc
     4501 atctaccatt atgtatctaa atctacttct gtaatatgct aatacttcat cattagcctt
     4561 gaaaagttct acagttttaa gtattaaatc atcaaagtca agtgcactat ttctttttaa
     4621 acggtcttga tatagcgcat aaatatctgc aatcttaacc attctattat ctgccatatt
     4681 gatatcttta aactgctttg gagtataaag tttgtctttt gctcctgaaa ttgcacttat
     4741 aactgctttt ggttcaaaca ctttatcact taaatttaat tcttttagac agtctttaac
     4801 caatgtaact tggtctgctg aatcataaat tacaaaactt ctattgtaac caatcttatt
     4861 tatatctttt cttagtatac gaacacagca agaatggaat gtacttatcc acatatcctt
     4921 agtttcagga cctatatttt gttcaactct ctctctcatt tcatttgcag ctttgtttgt
     4981 aaatgttata gctaatatat ttggtgcttg tacaccttta tcttctataa gatatgctat
     5041 tcttgttgtt aataccttag ttttacctga accagcacca gccagtataa gtactggacc
     5101 ttcagtcttt tctactgctt ctctttgagc aggatttaat gtatctaaat tcatatttct
     5161 cctcctgtta acagccataa atatatttta atctaaaata tgaattactt caaattataa
     5221 ttatgtatta tattaataga ttatttccaa tatattacaa ttaaataaca tttttccata
     5281 taatttattt cattgtttaa taaaaaaaat aaccttatga tttggggaat cataaggcac
     5341 ctttaagggg tctaggttaa gggttattat atataaatct atttttttaa aataggttta
     5401 gggcttaatt atttatttgt attaagttct ctttccttcc aatacttata caaatcttct
     5461 tctttcttaa ataagggtaa attcatattt ctatgtactt ttactaacca tggggattct
     5521 aggccagctt gtataatatt attttcattt ataaacacat tttctgcttt tccttcatca
     5581 atcaaaatcc ccttatccaa tacatatatg taatcacata tttcatacat taaattcata
     5641 tcatgactgg atattactat ttttatatta tttttattta atccctttat tatatctaca
     5701 atagacctag tacttactgg gtctaaacca gctgtaggtt catcaagtaa tacaatttca
     5761 ttttccattg ctataactga tgctattgcc actctttttt tctgtccata gctaagaaaa
     5821 tgtactggcc tgtctataaa atctattcca ttaacagcta ctagagcttt gtttattctc
     5881 tctttaattg ttttttcatc cataccaatg tttctcattg caaaggctat atcatcatat
     5941 acttttgaat aaaaaatttg tttttctggg tcttggaaga caatacctac attttttcta
     6001 aggttgtata aacttctctt atcatatttt aacttttctt ctttaaaaag aatttctcca
     6061 gatgtaggct ttaatatacc cattaaattc ataaataatg ttgattttcc tgaaccattt
     6121 gaacctatta tacctataac atttcccttt gaaaaatcca tattaatgtt taatagtgca
     6181 ttggtatttt tttcatattg atacgttaaa ttatttattt taaacatcat tatcccctac
     6241 tatgtgaaat ttaccatcat acaattttat gtctaatgat atacacatat catcatacct
     6301 tgtcataagt cttttgtaca gcatacttcc aagaatacct aaggatttat atgaagtttt
     6361 taaatttata tatccaaatc ttagctcttg tgacttgtat atttctgaaa attcttctaa
     6421 aaatatgaat ataaacctat atattaacat tgataactcg acaaaagtgt caggtaaatg
     6481 aagtttttta aaaaagaata ttaattgatt aaaaggtgtt gtaagtatac aaaaatacac
     6541 acaagtcaaa caagacatcg ctctaaataa cacatgaata gacatgtcaa tagacgcctt
     6601 tgatattcct acattaaaac taaaaaattc aaaactatat aataaatctg ctttattaaa
     6661 agatatattt acaagagtta ttcctatact aagaaataaa aagtacattg gtattctaag
     6721 taattttaaa taatttttta aatctattcc agccacacaa acaaccaaaa tactcattaa
     6781 aatcataatt ccacttaaaa taaatatatt ttgtattacc atagaagcaa tcagaaaaat
     6841 tactcctatg gctacttttt tgtttggatt tactttactc aatctatttg tatatgcata
     6901 tttatcaatt ataagcatga ttaacttctc tttccttttt gaagacctaa tatgtatcct
     6961 attacaccag cccctaaagc tgcttgtgca gagaataata aactttctat ttctccactt
     7021 ggtggctcat atggactgct aaaccatggt ttgtaatttg gatttatctt agtaatttca
     7081 ctttcagctt gaccatctgc tcctccatat tcagcacctg agttgacaag taatgggaat
     7141 ataataagag ctactactaa taaaagtaat aatatgttag tttttgtttt agcactcatt
     7201 agctttaact cctttatcat tattttctag tatgcttttt tctccgtttt ctgtaattaa
     7261 attatataca attacagtaa gtataccttc tgctattgct attggtattt gagtcataaa
     7321 gaaaattcct aaaaatttaa ttgctgaagc cattacccca cctgatgggt ctgggaaagc
     7381 cagtgctaat tgtattgaag ttattgtata tgtggctaag tctcctattg ctgctgcaag
     7441 aaatactgga atagcattat ttccatcttt tttctttaaa gccttaaata ttaggaatga
     7501 tacgattggc cctattatcg ccattgaaaa agcattagct cctaaagtag ttattcctcc
     7561 atgtgctaat aacaatgctt ggaatattaa aactatagtt ccaagaacaa acattacgct
     7621 tggtccaaac attattgctc caagacctac accagtagga tgtgaacaac ttcctgttac
     7681 tgaaggtatt tttagtgctg ataatacaaa cacaaatgct ccacataaag ctaataatac
     7741 tttcttttta gggtcttgct ttactatttt acctattgat tttaatccga ataagaaaaa
     7801 tggtataaat ataactcccc atgctataga ccattttact ggtaagtaac cttccattat
     7861 atgcattgca tttgatgtaa ctggagttaa aatgattaac ataagtgcag ctataacccc
     7921 taatttaata ttctgtttca tcttagtatc cctctctccc tttctaataa ttaaaacata
     7981 ataatacatg tctgatttat ttaataaaat aaaaaagcct aactcgatgg ttaggcttct
     8041 aattttccac aatctaaata caaacacata aaacaaacat atctgtataa ttattataag
     8101 aaactttctc catccccgaa gaaattactt taatatttta ggcaggtctc ctgacttagc
     8161 ttcatcctac tcctcttcct tcccatgaga tataaaaaat atcatcatag tggctaacaa
     8221 aagtttcgtc tgcatcacag tagcgggggc tgtaaaggaa tttaaccttt ttccctatta
     8281 atcttgctta aagaacctat aatattatat atttaatttt taaattcttc t
//
LOCUS       DAELNY010000164         8331 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.166, whole genome shotgun sequence.
ACCESSION   DAELNY010000164 DAELNY010000000
VERSION     DAELNY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.166"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..828
                     /locus_tag="KPE55_003011"
     CDS             73..828
                     /locus_tag="KPE55_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0494054.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            959..1981
                     /locus_tag="KPE55_003012"
     CDS             959..1981
                     /locus_tag="KPE55_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0494055.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2145..2702)
                     /locus_tag="KPE55_003013"
     CDS             complement(2145..2702)
                     /locus_tag="KPE55_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0494056.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2708..3784)
                     /locus_tag="KPE55_003014"
     CDS             complement(2708..3784)
                     /locus_tag="KPE55_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG0494057.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3785..4468)
                     /gene="modB"
                     /locus_tag="KPE55_003015"
     CDS             complement(3785..4468)
                     /gene="modB"
                     /locus_tag="KPE55_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG0494058.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWSGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4617..5429)
                     /gene="modA"
                     /locus_tag="KPE55_003016"
     CDS             complement(4617..5429)
                     /gene="modA"
                     /locus_tag="KPE55_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0494059.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5622..6551
                     /locus_tag="KPE55_003017"
     CDS             5622..6551
                     /locus_tag="KPE55_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494060.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6712..7161)
                     /locus_tag="KPE55_003018"
     CDS             complement(6712..7161)
                     /locus_tag="KPE55_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494061.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7364..>8331)
                     /locus_tag="KPE55_003019"
     CDS             complement(7364..>8331)
                     /locus_tag="KPE55_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0494062.1"
                     /translation="KNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGLEQLIK
                     NEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMILVHGI
                     GSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKFVKNKF
                     PEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEKRIPDI
                     LINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNSKKMYD
                     LVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2902 a         1369 c         1008 g         3052 t
ORIGIN      
        1 taaatatttc actaaaaaaa aacaaatgca aagctagtac caaaaataaa tcagaaaaaa
       61 gaggtgttgt agatgttaca aattaaaaat ctttcaaaga gtttccctaa tccatatggt
      121 gaacctaata ctatctttga aaatttatca atcgatatag aagatggtga atttgtaagt
      181 atcataggta gtaatggaac tggtaaatct actttattaa atataatatc cgggcttata
      241 aaagaatctt ctggtcaagt attattaaat aaacataact tgtctaattt agctgaacac
      301 agaagaactc aaatagtaag tagagttttt caagacccaa gtcttggaac ttgcccttct
      361 atgactgtta gagaaaactt gtctttagct cttaacaaag gaaaactatt aaatctaaga
      421 aaatgtcttc gttacaaaag agatttctta gaaaatttat tggaaggtgt atctcttgat
      481 ttaaaaaaat accttgatgt tcaagtaaaa tttttatcag gaggtcaaag acaatcatta
      541 tcactaatta tgtcgtgctt aacaaatcca tcagttttat tacttgatga acatacagca
      601 gcacttgacc caaaaacatc aaatgaagta attgaattga ctaataagat tgtaagggaa
      661 aaaaatataa ctacacttat ggttactcac aacttaaaac acgctcttca ttatggtgat
      721 agattgataa tgcttcataa gggtgaagtt gtattagatg taaaaggtaa agaaaaagaa
      781 caattaacag ttgaagaaat cttagaaaaa tttgaatatg cagtttaaaa taatatatct
      841 attgtattaa actcggcttt aaaataaagt taaaattaca ataattatgg ttactttgta
      901 ataattcata tgaatatatg aacatatata acaataaatt tttgaggggg atataaaaat
      961 gattaataaa aaaagattgg caagtttgat tttagctggt gctcttagta tatctatgct
     1021 tacagggtgt tcacaaggag gagactctgg taattcaaaa caagaaagta attctaaagg
     1081 taaagaagtt aaaaaaattg gtataactca actggttgaa catccagcat tagatgccac
     1141 taggacaggt tttgtaaaag cattagaaaa gaacggattt aaagatggag aaaatattga
     1201 tatagacttc caaaatgctc aaaatgacat gcctactaca caaagtattg cgagtaaatt
     1261 tgcatctgat aagaaggact taatatttgc tatatctact ccatcagctc aggcagcatt
     1321 taatgctact aaagatatac ctatacttat tactgctgtg agtgaccctg ttgctgctgg
     1381 attagttaaa actcttgaaa agccaggcac aaatgtatct ggaacatcag attttgtttc
     1441 tgtagacaaa ggtttagaac ttcttaaaat atttgctcct aaggcaaaaa ctataggagt
     1501 tatgtacaat actagtgaag taaattccaa agttcaagtt gatgctttaa aagaatatgc
     1561 atctaaaaat ggatttaaag ttgttgaaaa aggtataact acttcaaatg aagttaacca
     1621 aggtatttct agtttagttg gcaaaataga cgtattatat gtccctacag ataacttagt
     1681 ggcttcttct atgcctatag tctctaaaat agctactgag aataaaatac ctgttatagc
     1741 agctgaatct ggtccagttg aaaaaggtgc tcttgcttgt caaggtatca actacgaaaa
     1801 actaggttac aaaacaggtg aaatggcagt taaaatttta aatggcgaat cagtatctga
     1861 tatgcctgtg gctacatcag atgatacaga tatcattgtt aatgaagata tattaaaagc
     1921 tttaggtatg gaaaaacctt ctaatgaaaa tatttcttat gtaaagacta aacaagaata
     1981 gaaagatatt ttctatatac gattaatatg cctgtttcta taaattaatt tatagaaaca
     2041 ggcatatatt tttattttaa tgtattatct atttaaagat tatatgtaaa tgctcttaat
     2101 ttgtttgtat aaattttact tgtataaact ttatacaatc atatttattc ttctattttc
     2161 tttattactc tacatggatt tccaacagct acagtatttg gtggtatatc ttttgtgact
     2221 acacttcctg cacctattac tacattatca cctatagtaa ttcctggtgt tatgattact
     2281 cccccaccta tccatacatt atcacctatt tttataggag aaccatactc tatgcctgaa
     2341 tttctaagtt gtgcatcaat tggatgatag gctgtataga tttgaacgtt tggagctaac
     2401 attacattgt cacctatttc aattttacat acatctaaaa aaatacaatc ataatttgca
     2461 aagaaatttt cgcctacatg tatgttatat ccataatcac acctaatatt ttgttctaca
     2521 tttatttgct tgccaactga accaaatagt tgtcttaaaa tatcttctct tttttcatat
     2581 tcatcctcta aagtattatt aaacaatcta gttaattttt tacaatactc tctttctttt
     2641 actaacaatt catcatttgc atagtatcct ttcccagaca acattttttc tttttcagtc
     2701 atatagttta ctcctttttg ttataattgt atctaaagca aaataaatat tcctgtggaa
     2761 aatctaaata tattctgtca cctttcttaa atctcatttt acttttttcc aattctattt
     2821 gaataggtac acattcgtta gataaatcag tttttctaac atatatagta tatgtaaaag
     2881 gattttctac aatgttaatc aattcgaaac ataacttttc atttaaatct tcattagaat
     2941 ccaagacctt aatatgatgc tccctgattc ccacatattc tatattatca cctatttctc
     3001 tatttaatgt caactcgcat ccccagtctg tagcataaat agtattttta tccagtctat
     3061 ttaatttgga tatattttta caccctgtta tcttagcttc tataaggtac tttggagatt
     3121 caaagagctc ttttttaggt ctctttggaa gaccttctcc tttgttgtac actataatat
     3181 catcacaaac tcggtaagct tcttcaatat catgagtaac aaatagtata tctcccttgt
     3241 aatcttttaa catattcata agttcttttt ccatattgct tcttaaatga tggtccaatg
     3301 ctgagaaagg ttcatctaaa agtaatatat ctggagatgt tgccaatgct ctagctagtg
     3361 ctaccctttg ttgttgacca ccagataatt gccaagggta tctcccctca aacccattta
     3421 atttaagtat atctaagtac cttgctacta tttccttttt ttcactctta cttagtttta
     3481 aaagcccaag ttctatattt tgacttacag tcatatgtgg aaacaaagca tagttttgaa
     3541 ataaaaatcc tacctttcta tcttgtgttg ataaatttat cttcttttca gaatcaaata
     3601 gaactctatc atttaataca attttacctc ttgttggttt ctcaagccct gctatacatt
     3661 ttaatgtcat actttttcca gaaccagatt ctcccaaaaa ccccagagta cctttttctt
     3721 gtttaatttc tacctttagt ttaaaagagg ataaatcttt ttcaatatcc acgtataagc
     3781 tcaactatcc acacctcttt cctataattt tctgctgaaa ttccgaccaa taatttagta
     3841 ataaaatcat tgctatagat attgctacaa taatcataac ccacatcata gctttattca
     3901 tatctccgcc ttcaacagca aaaaatatag ctattggcat agtttgagtt tttccaggta
     3961 tatttccagc tatcataagt gtagcaccaa attctccaag agctctggca aacgacagca
     4021 ccaatccacc aattattcca gaccatgcta atggaactgc aatcttaaaa aatactttcc
     4081 attcattaag acctaatgtt ctagcagctg acaacatatt attatcaatc tgttcaaaag
     4141 ctgaccttga agttctatac atcataggaa aagacaccac tactgctgaa attacagttg
     4201 ctgtccaact aaatattaaa gtcctatcga aatttaataa aaactttcct ataaatccat
     4261 tcttcccaca tagtaataat aaaaagaaac ctacaactgt tggtggcaaa attagtggaa
     4321 gtgtaaatag tccatctata agacccttcc acttacctct atagttagcc ataatatatg
     4381 acacaaatat tcctattaca aaagttatta ttgttgacaa aatagatgtc ttaagtgata
     4441 tccataaggg tgaccaatct gttcccatct ttccctcctt aattttaaat ggaaatagtg
     4501 aaactaataa ctctattaat ttcactattt ccattttatt ataatacaca attattttca
     4561 attttcattt atttatatat aatttatact taattacaca taatttattt ctaagtttat
     4621 tctacttttt tatatccaaa ttcttcaaaa atcttttgtc ctgactcact taataaaaat
     4681 tcttcaaatt tcttagcagc atctacgttt ttactagctt taattactga aactggatat
     4741 gtaataggcg aatgagtttt ttcgtctgtt ttttcaacta cttttatctt atcattattg
     4801 acagtatcac tgaagtaaac gaatccaaca tcagcatttc ctgattgcac ccatgctaaa
     4861 acttctttta catcttttgc aaatactagc ttatctttta acttatcttt taaatttaaa
     4921 ttagttaaca cctcatctgc atacttacca gctggaacac tttcagcttc accaacagca
     4981 attttcttca ctttatctgt agtcaaatct ttcatacctg atacactact gtcagcagaa
     5041 gaaataagaa ctaaatcatt ttttactaaa tctttcatag tatcagaaac taataacttc
     5101 tcttcatcca aagctttcat ttgtttttga cctgctgaaa taaataaatc acaaggtgct
     5161 ccttgttcta tttgttgttg taatgagcct gatgctccat agttaactgt aagttttaca
     5221 tttgaatcaa cttttttata ttcttcttca atctttgcca ttgcttcttt taaacttgca
     5281 gcagccgata tatttaactc tactgaatca ctacttttag ttgttttttc ttgttttcca
     5341 tcatcttttt tgtcttcatt actattgcac ccaactgtcc ccaaagttaa acatgcaact
     5401 aatcctaata ttcccaatat ttttttcata acaatctccc tttttgtaaa tttgatacac
     5461 attttaacat gttttattat gttttatcat gttttgttat atatagttta attatatata
     5521 catgataata aattttcaag taatttattt acactacttt taaatatttt gctacatttt
     5581 ccttttttta atgttaaaat aaccttatat ggaggtggat tatggactca ttatcatctt
     5641 tgacacctgt cgaggtagct gaattattaa agataacaaa aaatactgta tatgaattaa
     5701 tcaaaagagg tgaacttcct tcttataaag tagggaaaaa aataagagtt gatactaaag
     5761 atgttgaaga atatatcaac agccaaaaaa ctggtaaagt aaaggataca catttcaaca
     5821 ataaaaatat agatataaat aataatttca aaattattat ttctggtcaa gatgtaatat
     5881 tagacatttt agctaggtct gttgaaaaaa aattggatgg aataagtaca tttcgctctt
     5941 atataggtag ttataatgct ttgtatgagt tatataatgg cagagtttct atagcatcat
     6001 cccatctatg ggatttcgaa acggatgaat ataatagcac ttttgttaaa aagctattgc
     6061 caggaatacc atgtgtactt ataaatctag cttatagaat gcaaggtttc tatgttgcta
     6121 aaggaaatcc taaagagata acaacctggg aggatttaac aaaaccaagt ataaccattg
     6181 taaataggga aaaaggttct ggaactaggg ttttactaga tggtaaactt agacttctaa
     6241 actttaatgg taaacatata aaaggttatg ataatgaaga attatcacat cttggagttg
     6301 caagtactgt ttctcgtgga atagcagatg ttggtttggg aaatgaaaaa gctgctttac
     6361 aagtaaataa tatcgacttt attcctcttc acaaagagcg ctatgattta gttattaaaa
     6421 aagaagactt acaaaatcca gtttaccaaa ctataattaa cataataaac tctcctgagt
     6481 ttaaagctga acttcaaggt cttggcggat atgatttaac tgatactgga aaaactatag
     6541 caaaagttta agattaaaat cgcaatctat tttataagat ttataatttt tcacaaaatt
     6601 tataacttaa acacgaaata aattgcgatt ttagtgtctc aaaaaattta tgtctttcat
     6661 tttatcatcc aatacaaaat ataatataat taattgtgca atcatattat attaatatat
     6721 atttacataa tagtattttt ctttaatgta gattaacata tgatatgttt taaacatcaa
     6781 tccccttgct ttcttatata tatttggcaa cacatatgag tttaataaat tatatacaat
     6841 gttgctttct tcttgtattt tttcaaccaa caagttattt ataaaattac ctttctcatt
     6901 ttcaataatc tcactccaat ttatcttaac ttttaatata tatcttccca ctaggtatac
     6961 acactctaca ataaataata aaaatacaat gcagtcagta cctgttgtct tatataaatt
     7021 acccgatact aatggctttg cgaagaaatt ataaaaattc aaagtatcac ccgtataatc
     7081 aaaaagtatt aatagcactc ctagaactct caatacttta atattcaaca tgtttttatc
     7141 tttattattt ttgtttttca tatatactac ctctcttttc tttagatttg attgtaatct
     7201 aatactaact cttttatttg aggtataaat gaatttaatt ggtagttatt ttcattttta
     7261 ctagaaatta attcacatta ctaaacaaaa cttatattta aacataaaaa tagcctatct
     7321 atcttttaat aaatacaaaa ataaataagc tactaatatt caattaactt ttatcactta
     7381 aaacattact atctataaat ttaaatattt tattgaagta tactgtacta tcatgttcaa
     7441 atccatcaac atgaccaata ccttctataa gccataggtc ttttttatca tgtttaacta
     7501 aatcatacat tttcttgcta ttttgataat tacaaacagt atcttttgtt ccatgtataa
     7561 acagtatggg tacattagat ttctcaactg actcatatgg ttttacatca ctgtaccaaa
     7621 atccagattt aaacttatta tataaatctc cacaagtcac cacataattg attaatatat
     7681 ctggtattcg tttttctagt actcccattc taattgcatc tttcatttca gaataaggag
     7741 aatccagtat ataaaaatcg accttatcat cttttgaatt tatctctgaa tgcatagcag
     7801 ctgtccctgc ccccatggaa aatccatgca ctcctaatct cccttcagga aatttatttt
     7861 ttacaaattt aactagacta tctaaatcat acctttcata aagaccaaat gtgtaattat
     7921 ctccaccact attaccagta tttctttgat tataaactaa tacattataa cctttatcta
     7981 aatatctata tgctaccttt aacatttcat agtaactact tccaattcca tgaacaagta
     8041 tcatagtatc tcttgtcttt atatttgaag ttataaaaag actttcaagc ttgtaattat
     8101 tagaactttg tatcattaat tcagatgtct tgtagttttt taatttctcc agtggtttat
     8161 cttttctctc agaaaatatt tttactatat cttcgttttt tattaactgc tctaaaccaa
     8221 ctgaactatt atacactaat tttccaaata tataagatac aattaatagg attaatagaa
     8281 acacaattga tataattata aatttatttc ttaaactaat acaattttta a
//
LOCUS       DAELNY010000165         8216 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.167, whole genome shotgun sequence.
ACCESSION   DAELNY010000165 DAELNY010000000
VERSION     DAELNY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.167"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(174..356)
                     /locus_tag="KPE55_003020"
     CDS             complement(174..356)
                     /locus_tag="KPE55_003020"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494063.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            complement(685..1185)
                     /gene="tcdE"
                     /locus_tag="KPE55_003021"
     CDS             complement(685..1185)
                     /gene="tcdE"
                     /locus_tag="KPE55_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG0494064.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            complement(1307..>8216)
                     /gene="tcdB"
                     /locus_tag="KPE55_003022"
                     /gene_synonym="toxB"
     CDS             complement(1307..>8216)
                     /gene="tcdB"
                     /locus_tag="KPE55_003022"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG0494065.1"
                     /translation="KSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVWIGGQIN
                     DTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFRENLNDPRF
                     DYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEIDELNTY
                     IEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISALKEIGG
                     MYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDE
                     EVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNL
                     IVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMELGKYLRV
                     GFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNISQSTEQ
                     EMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKISSLARS
                     SERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPGDGEIQE
                     IDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLAKEDISP
                     KSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSANQYEVRI
                     NSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELSTLLQEI
                     RNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIKDEFKLI
                     ESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETEKTIFSE
                     YANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYNSSKESL
                     SNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLPIIATII
                     DGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASGFSILLV
                     PLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMPQDDLVI
                     SEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYDVLEVQK
                     EELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEFYWRYFA
                     FIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSGGTYALS
                     LSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIEENKIIL
                     NSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKILMLNSNH
                     IQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLISKVYMD
                     DSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKLNSVHLD
                     ESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANFIISGTT
                     SIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGDISSTVI
                     NFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYINEKINVN
                     INDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNFSDKQDV
                     PVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMLSGLIYINDSLYYFKPP
                     VNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFSTEDGFK
                     YFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYFSPETGK
                     AFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKHFYFAEN
                     GEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTAVVGWK
                     DLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIE
                     SGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGE
                     DVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEKGIMRTG
                     LISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLD
                     ENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISE"
BASE COUNT         2738 a         1385 c          825 g         3268 t
ORIGIN      
        1 tataaccaca tttattcctt ctgaaattta aattgatttt cttgttttta aactttgaaa
       61 ataaatctta tacactcctt tctatttttt aaaaataaat taatattgat tttttataat
      121 ttaagttttc atatttaaat cacttcctaa catttcaatt taaagatact caattagtta
      181 attgactctt ctattagata aggagattgt ctgatagtaa tttgtaaaca tatcttatat
      241 ttttttaatg tttttatttc tttatatatt tttcctataa cttttaaact atcaattaaa
      301 tcatttatta aggtttgata cttattttct atatcatttt gtccttcatc acgtattttt
      361 gtgtttttga ataatttcca tcacaattta cacatatttc tatgtttaac tattatagtt
      421 tgtgatatct gtaactaatg tagcttaata gattttacca actgaccatg caccatcttt
      481 gtgaaccaat gtttgatata ctaccttgtc atctatatag taatgaacac ttgcttcgaa
      541 attatatgta tttaatactt gtgtgtctct tggcatctgc tccctcgttt tcattattag
      601 tttcatgaat tatgataact ttcagctatt cttatttgga taacactttt tatttgttaa
      661 tatctttatt atatctacca ttacttactt ttcatcctta gcattcattt catctgtcat
      721 tgcatctagt aaaattgcta ttttttcctt taatctctta ggtactggta atccacataa
      781 gcacatattt tttaatatac ttacactttc atataaaata aataaaatag agaaaaattc
      841 agatattcca agatgtttta gtcttaaaaa attgatacaa tcttgtggta acataaataa
      901 aaagttaaac tttgtaagaa tgtcaactac tgataaaaaa aatatacatg ctatcattgc
      961 tacttttctg attcctccat ctattccaaa actagaatta aatttacgac tttttattgc
     1021 acttaaacat cctaataatg tatcaaatga tatagttaaa attactaact ttataaatat
     1081 atgctctgat aaaaaagata ttgtcatatt cataacgcct cctaggttta tataaaaaaa
     1141 tattttgtta ccattagaaa tataaaaagg tgaactactg tgcattcatc atagtccacc
     1201 tttttattgg taaatcttca tattagatag aacactttat gtagtgtatc attttgttta
     1261 tttttatata tttaagtata agaggatata tttaacatat ttttatctat tcactaatca
     1321 ctaattgagc tgtatcagga tcaaaataat actcctcacc atcaataata actgaaccag
     1381 ttgctgcaat atattcatct gtaaaataat atctcttttc atctaaatct aaccaaccag
     1441 tatagtttat tgattctccc tcaaaattct catccaaagt attttgatgt gcaaagtatt
     1501 taaatccatc tggtgtatta aatactccaa tctgcatgac accatcttca ccaaaataga
     1561 acatcttatc ttctatattt atataaccaa attgcatttc accattctca ttaaagtaat
     1621 aattattatt ttcaaatgat ataagacccg ttctcattat gcccttctca tcaaaataat
     1681 attttatatc atcaattaaa ttaatacctt tgcatgcttt tttagtttct ggattgaaat
     1741 aatatttatc actttcattt tccatatcat atatccatcc agtctcaatt gtatatgttt
     1801 ctccaaaata atatacatct tccccaactc taactaaacc actatattca actgcttgtc
     1861 cgtaaatatt atcatttaca gtattagcag gtgcaaaata tttatatcca tctgaagtat
     1921 caaatacacc aatttgaact ataccattat catctatata gaaataattg tcatctatgt
     1981 tttgtactcc agattctata attccagagt cagagaagta gaagacttta tcatttatag
     2041 tgacaaatcc aacttgcata attccatcat cattaaaata atattgacca tcatttatta
     2101 atgacaaacc tatatatgct tctgctgtat cttcatcaaa ataatacttt gaaccatcct
     2161 ctaaatcttt ccatccaact acagctgtaa atgaatcatc aaaatagtaa attttattat
     2221 tgaaatttaa tataccagaa tatgagattt cttcaccttc ttcatttcct aaatcttcat
     2281 tatgatgagc aaaatattta aatccatctt ctgtattaaa tactcctatt tgcatttctc
     2341 cgttttcagc aaagtagaaa tgcttgccat ctatttcagt gtaacctact ttcataacac
     2401 cagaatcatc aaaatagtgt ttattatcat ttatactaac aaatcctttt tgcataactc
     2461 catcagaatt gaaatagtat ttatcatcac ctatttgatt tagaccttta aaagctttac
     2521 ctgtttctgg gctaaaatag tgcatttcac catctaattc tttccattct acagctcctc
     2581 tataattatc atcaaaataa taaatatttt cgtcaataat taattttcca gtaaaatcaa
     2641 ttgcttctcc ttctaggttt tcatcaagtg tattagctgg ggcaaaatat ttaaatccat
     2701 cttctgtact aaatacacct gtttgtaaca ctccactttg gttgaaataa taatttttgt
     2761 catcaattat tgtctctcca attgaagcag ctccaccatt aattggatta aagtagtatt
     2821 tatcatcgcc tacagtcaca aatccagtta tcaaattatt tactggtggt ttaaaataat
     2881 ataatgaatc attaatatat attaatccag atagcatcat tccaaagtta ttaatataaa
     2941 atttctcatt atataaagaa actagaccca aatcatagcc aatcaatcca tcctcataat
     3001 atgaaggtgt aaatgataag attatttcac ttacaggtac atcttgttta tcactaaagt
     3061 taaaagatag cttatttgcc aaagtcttat ctttaaaaac attaacgaat cttattttaa
     3121 cttgtgacac cttattttct tcactagttg acataagaat aaaatcatta ccatcattac
     3181 tccatacata tcgtatagat agatcattga tattaacatt tattttttca tttatataat
     3241 ttgcatctaa tacaataact tctggataag tattatttgt ttcatataca ggcgttatat
     3301 ttatttcatc tgtataaata tttggtgaaa ttacaacttt attaacacaa ctgtctattc
     3361 cataaagata cttttgagag aaattgataa cagttgaaga tatatctcca gaatcatcta
     3421 aatcataatt tggttccact atcatatttt gtctatttcc tacatataaa gtataattag
     3481 tttctagtgt attaaactta atgaaatatg gttgtatatt atcattttca tcacaaataa
     3541 actcaaattg gccaatagaa gtagtaccac ttattataaa attagcatct aatataaact
     3601 taatattaga ttgtaagaaa ttaacgaaaa tactttttat attcatactt tctaaaaagc
     3661 tcattaaaga atctgaagta tttgtattac cttttctatt catgaacttc aaaatctcag
     3721 ctactccact ttcatctaaa tgcacactat ttaactttat agttttttca tcttgtagag
     3781 tcaaagaaag agagattttt atatcatcct taagataata acctgttaat atattaacat
     3841 tatctttagt tataactttg acatctttca aattattact ataatatcca aatgaaggct
     3901 tactatcatc catataaacc ttacttataa gaactacatc aggtaattca gatacaaata
     3961 aaccttcttt tgttgaacca ttaataaaac cattctcttt tccttcacta tctacaaagc
     4021 tatatggtat atttttctgt aattcgctat tgaatcctat ataatctatt ttctgttgaa
     4081 tatgatttga atttaacatc aatattttta attcgccaga aataagtaat ttatatgatt
     4141 tagataataa atcaacttct ataattgcat ttattccttc taaaattgaa aatgttaaag
     4201 aaacaaatcc attacttcca tttacctcac cagaaaaatt aatctcatgg ctatttaaga
     4261 taattttatt ctcttcaata cttagtgtag ataaaatacc ttctattaaa tcaccttttt
     4321 taattttatc agattctata gttacatctc tcacaacatt atcaacatct ataatccaaa
     4381 catcactttc acttaattct atatttatac ccatattata ttgagaaaga gacaatgcat
     4441 aagttcctcc tgaaccatag aaagaatatg ataatttttc tcttatatat tctgtagtta
     4501 ttattggaac tataaaactt ctagtattac tatctaaatt tattcttata ttagtatctt
     4561 catatcttgg ttttaatgtt gttattaaag catcagctat aaaagcaaaa tatctccaat
     4621 aaaactcacc ttcatagtta tctcttatac ggtctaacag ttttgtgcca tcattttcta
     4681 agcttcttaa acctggtgtc catcctgttt cccaagcaaa tactctattt ggagcattag
     4741 gtaataccat taaatctttt gacaaatcaa gttcttcttt ttgtacttcc aatacgtcat
     4801 atatagataa gtgtggctct ctatatgtta ttgatggtgc tgaaaagaag tgatctatat
     4861 catcagttac agtatgacct gaaccacctt ccattctcca gatttcacat ttacctaaaa
     4921 ctattgaatt attattaaaa tctatttctg atatcactaa atcatcttgt ggcatcatta
     4981 ttttatcatc taataaagta aatactcctt cagtttcaac taatgaaaca tgtttaaaat
     5041 aatctacaac ctttgttgcc ttatctcgaa gtacaagttc attgtttact aagcttggta
     5101 tacctgctga aattcctgct aaaggaacta aaagtatact aaatccacta gctatcccca
     5161 aagatgaagt aatgattgca gttgtagctg ttgttaaatt tactgccatt atacctatct
     5221 tagcttctat ttcttgtctt aataatgggt cactcgtttc acttagctct ttgattgctg
     5281 cacctaaact tacaccatct ataatagttg caattatagg taatccttca gataatgtag
     5341 gaagtaagtc tatagtttca tctaatgcag ttgatactaa ttcaacaact ttggctgcat
     5401 ctgtaatagt atttaaacca gtactaaata attgagcgta aacttggact ttcattgcta
     5461 cacttaaatt actaagagat tctttagaac tattatattc tattaatgat tgtataaaaa
     5521 atgcagcatt taaagtattt acttcgtgtg tagtatctaa atttactttt tttactaact
     5581 taccatttac agtatcaaat atagtacctt ttatcttaga aatctcttca gttatatgat
     5641 tagcatattc agagaatatt gttttttcag tttctacaaa tatagattct ccagtttctt
     5701 tattaataaa tcttatacta aatccctcat cagtctctga tatgtcctca aaagatataa
     5761 aatgagaatc ttctaattca ttctgttgtt ttaagtcaca tagtgcatca gaaatagatt
     5821 ctattagttt aaattcatct tttatataat taatagagtc agaagttaaa ttcttagctt
     5881 cttcaatcct ttcctcaaca atttgcgtat ctatatttga aataacattt atctcacatt
     5941 ctgttaacat tactttttct tctagttcaa tatcacttga attagaatta tttctaattt
     6001 cttgtaataa tgtagatagc tcaggtaaat ttttagattt tactgtaatt ttattttctt
     6061 taggattaaa tgatatatat tcttttgatg aaatatcctt tataatactt tcttctttgt
     6121 ttatccattc accagaatgg tctaataatt ctcttcttcc ttcactattt attctaactt
     6181 catattgatt tgcacttact ataatagagt cttgacttat agatggcatt aattctgata
     6241 ttttatcttt aactttaagt aataattttc caggataagt ctcctctacg ttgatagagt
     6301 agctaaacat attacatcct aataaattta tttctattga cttaggagaa atatcctctt
     6361 tagctaaatc tattgctgct tctatttctg tggataatga atctacatca aaacctgcaa
     6421 atatatcagt attaaattca tctttaccat gaccaataaa tgttaattta atcttaggtc
     6481 tatcagaaat tatacttgga attttatact tgtctatttc ttgtatttca ccatctccag
     6541 gattataata atatgcaatt tctgaatctt ctatattttt ctgaaacagt acactatcat
     6601 aaggagtctt tgcaaataag ttacatgctg cttcataact aattttatct ccttgtaact
     6661 gaacaatata gtgtatatat cctctctctg aacttcttgc taatgaagat attttttcta
     6721 aaagatactc cttgtcaact actatatttt gagaaaaatc aagattatca tcttcaccaa
     6781 gagaaccttc aaaataattc cttttatatt cttcaaattg agctttagct cttgcatcgt
     6841 caaatgacca taagctagcc atttcttgtt cagttgattg agaaatatta gttttagaga
     6901 tttcaaagtt tcttaaatca gcttctatca aatggatatt catactgcct tctttaaaca
     6961 ttaataaatc ttgataagct gccgcatatg cttcagggcc acttaagtta atagtagttt
     7021 taacatctgg gaagaaacca actcttaaat actttcctag ttccatcata aatctaccat
     7081 tatctgcatt agcttcagcc attatactat caataaaggt attcgttgta gtattaaaat
     7141 cattatcctc gctaatagct ggatttaaac tattattcaa tattttatat ctattctcga
     7201 tttgttttac tattaaattg ctacaatatg agtctttcac agaaattagc ccttgattta
     7261 taataccctt actattaaat gcaattttaa cttctagtgg tgatgcctcc atatcaccaa
     7321 gtgatgagaa tatttctgat ttatctgact tagaagctag aacagattca aaactacttt
     7381 gaacttcttc gtctaacatg tcaaaatgtt ctgaggtata ttctggtata tattctttgt
     7441 atttcattat agcttctaac tttgtcattt cccaaaaatc cactgttact gaactaggtt
     7501 tctctataga ctcaaataag tctggttgta ttcctggtaa catatcaaca tctaaataca
     7561 taccaccaat ttcttttaat gcagatattc ttaatatgtc agaagcagca gctaaattcc
     7621 acctttctac caactcttgt tcatataagt tgaatgactc tccattttta aattcttcaa
     7681 agtttctaac atcatttcca ctattctgtg taattttatt taaggattct tcaatatagg
     7741 tattaagttc atctatctcc tttgaatact catttgaaag atatgtcttt acaatatcat
     7801 caattataag ttcaggattt tcttctcttt gagctttata gtagtttatg aaatttttct
     7861 gtttatcata aattatttcc atacgttttc tgaagaattt attatagtca aatctagggt
     7921 catttaagtt ttctctaaat gattcaagtg tatcatttat tgctgattct actacagttt
     7981 ttttcaatgt gtttatcaaa aatgcattac tatcataaaa aacattaaca ttataatcac
     8041 tatttacatc tttccattga tttatataat taatagcagt gtcatttatt tgacctccaa
     8101 tccaaacaaa atgtaaattt ttctcaactg gagttaaatt attattcttt agctctaata
     8161 cttctgtaac tagatattcc ttaaattttt ttaaggcttt atttctacca gatttt
//
LOCUS       DAELNY010000166         8204 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.168, whole genome shotgun sequence.
ACCESSION   DAELNY010000166 DAELNY010000000
VERSION     DAELNY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.168"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            267..1262
                     /locus_tag="KPE55_003023"
     CDS             267..1262
                     /locus_tag="KPE55_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0494066.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            1240..2103
                     /locus_tag="KPE55_003024"
     CDS             1240..2103
                     /locus_tag="KPE55_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0494067.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            2100..2894
                     /locus_tag="KPE55_003025"
     CDS             2100..2894
                     /locus_tag="KPE55_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0494068.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWKQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLAMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3179..4558
                     /locus_tag="KPE55_003026"
     CDS             3179..4558
                     /locus_tag="KPE55_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0494069.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVSQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            5006..5719
                     /locus_tag="KPE55_003027"
     CDS             5006..5719
                     /locus_tag="KPE55_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494070.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(5991..7529)
                     /locus_tag="KPE55_003028"
     CDS             complement(5991..7529)
                     /locus_tag="KPE55_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0494071.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         2895 a          928 c         1265 g         3116 t
ORIGIN      
        1 tttatagata actgacatat gacaacaaat agtatttaaa atatatatta ctttgttaaa
       61 ttatggtgta gtaggcttta atctgctaca cttttattaa ttttgtatag tattattttg
      121 ataattattt tgaaatagag agcattaatt tttctagtcc acaggagttt tctgtttttg
      181 tgctgttaaa tataagcaca gaaaaaggca gatatatacc tgtggacttt tttttatata
      241 aattatgaaa tatggaggta gttaaaatga gtataattga acttaatgaa ctgactaaat
      301 cttatagaag atttgagaaa aaggcaggat ttatggggag tgtaaagtct ttatttaata
      361 ggaaatatac agaaaaaaca gctatatcgt catttgattt aaagatagaa gaaggagaat
      421 ttgttggatt aataggtcaa aatggagctg gcaaaactac tcttattaag atgttaactg
      481 gtattattgc accgtcttct ggagaaatat ctgtaatggg ttattaccct aacaaattag
      541 aaaataattt taaaaaacaa tatgctgttg ttatgggcca aaaaagtcaa ctattctttg
      601 aattgacacc agcagatacg tttcttttat ttaaagaatt atatgatata tctaatgaag
      661 aatataaaaa aaacttggat tactttatcg aattatttga ggtatctgac tatttaaatg
      721 ttcaagttag gacgctttct ttaggagaaa gaatgaaaat ggaactaata gtagcccttc
      781 tacataatcc aaaaatatta tttttggatg aaccaactat aggcttagat gctatagccc
      841 aaaaacaaat taggtatttc ctaaaagaag taaatgaaac aaaaggaact actattatac
      901 ttacttctca ttacatggaa gatattaaga gtctctgtag tagaagtatt gttgtagaaa
      961 atagtcgtaa aatatatgat ggttctacag agaaattatt taattatttt caaaaatata
     1021 aaaaaataac agttagtttt tccaatgaaa ctaacattgt atttccaaaa gattgtaaag
     1081 ttgttgaaca aacaaatcat aagacatcag taattgtgcc aaaagaaaga ggaaatgaaa
     1141 cgattaaatg gatttttgat aaatatgatg ttaaagatgt aatgattgaa gaagaagata
     1201 taggtagtat tgtagagaga atatataaga aaggaagtga tgagtattat gagcaactat
     1261 aatgaagcaa gttcaaatta ttcaatgttt tataaattta aacagagtat tagaaggtat
     1321 tatgaagtag ccaaaattaa ttttaaagtg caacttatct ggagatttga tgtggtggtt
     1381 aatatggtgt ttacgattgt aaaaatttta tttgcttata ttttatggaa agcaattttt
     1441 ggtgaaaata aaatagtctc tggttttaca tttaactcta tgttatctta ctatataata
     1501 agctcatttt tagcaggtat tgatatgtca aagagtacca gtgaagagat atgtaatcgt
     1561 gtaaaattgg gcaccttttc aaagtacatg attattccaa ttaatgttca aaattatttt
     1621 attgctaaaa attttggaat gacatgtttt tattttgtat ttaatatgat ttctgcattt
     1681 gtatggattt ttatatttaa aatagatttt gcctttacat caaatccagt tcaaatatta
     1741 atttcaattt tgatgatttt tctagggttt atttttatga tgaaactcca cttctttatt
     1801 gggattttgt catttaaatt tcttgagatt agttttttta gaatgataat tgaaaatatt
     1861 gttgaattta ttacaggaac aatgatacca cttgtactct taccagaaac tatagtaaca
     1921 gttatgaagg tatttccatt ttattataca acatatttgc catctatgtt actaataggt
     1981 agaaacagca atgaggcaat acaaggatta attattctag cattatgggt tttatttttt
     2041 tctgtagtaa ataaagttat gtataaaaga atgagaacat tatttgatgg ggtgggtata
     2101 tgatgaaaaa aaatttgaaa ataatattta tgttattaaa aatgaaatta tcaaagatga
     2161 tggtatttag atttgatttt tttggagcat tttttgtaga tagttcatta tttatattac
     2221 aacttctaat gtttaatagt atatattctc atgtagactc aataggggga tggaaacaag
     2281 gagagatgtt aatatttata ggaactttct cattaataaa tgcgataaat atgacgattt
     2341 tcttttttgg tatttatgat attccacgaa aaatccagga aggagaatta gactactata
     2401 ttacaaaacc attagatcca ctatttaggt taacatttga aaatattaat attggttcat
     2461 caccattaat tctagcaagt atacttattg ttttatatgg aatatctgag ttaaactcag
     2521 agataacttt agctatgata ataatatata ttttcatggt gttattgatg acattacttt
     2581 tttatgatgt atgtatcata cttagaacag tttcattttt tgttatatca tcttctgcga
     2641 taatgagatt agaagataat ttattgccaa tgaatatgaa aattcctggt gtaatatatg
     2701 aaggtgtttt taaagtatta ttctatttaa tcttaccata tggaattatg tcgacaattc
     2761 cgactcaaat tttggctggg acaataacaa ttaatggatt aatatactca attttaattg
     2821 tatttttgtt tacattgttt atgtggagat tttggaagtt aggtatgaga cattataaaa
     2881 gtgcaagtag ttaataaatt agtaaaaata gacaatgtat aactatatat aaatatgttt
     2941 catatgacac taaactatgt gaacaagtat ttgcgctaaa ggcactgaaa gctataaaat
     3001 tattaaagta aggatttaaa agtttcttta gtagaaatat aattgtgtaa aaaagcaaga
     3061 ttaattaaaa ctatatccaa ataattagat ataattctta tgcagaaagg aggtctgtaa
     3121 atgtctaatt gtttggaata tattttaaaa ataattaatg tacagaaaaa taaacaaaat
     3181 gagtgatgta tattattcag aaaatcatga acaagataat ctttatgagc aagataattt
     3241 actggcacaa agatttaatt tatttaccct attgaagttt gcatttccta ctatttttat
     3301 gatgattttt atggggttgt atacaatcgt agatactata tttgtatcaa gatttattaa
     3361 tacaaatgct ctatcagcaa ttaatattgt atgtccaatt attaatctta tagttggaat
     3421 tggaactatg cttgccactg gaggaagtgc tattgtagca cgtaagatgg gaaatggtga
     3481 tattgaggga gcaaataaag ctttttcttt aatcgtatta tcaggagttg taattggtgt
     3541 aataattact ggtataggta ttgtatttat ggattatatc attatatggt taggagcaag
     3601 tgaagtttta attaaatatt gtcgttcata tctttttatt atcttggttt ttgcttctgc
     3661 aaatatacta caagtattat ttcaatgttt atttgtaaca gctggaaagc ctcaatttgg
     3721 attatggcta gtgttaggtg ctgggtgtgc taatataatt cttgactatg tgttcattgt
     3781 tctgttacaa atgggtatta gtggagcagc acttgcaact ggagtaggat acctaattcc
     3841 taccgtttca ggtatggttt tcttttcaaa aaacaaaggg ttacttaagt tttgtatacc
     3901 cagactagat aaaagaatat tatttgaagc ttgttttaat ggttcatctg aaatggtcag
     3961 tcaactatca acagcagtta ctactttttt atttaatgct atgatgatga aattaattgg
     4021 tgaagatggg gttgcagcta ttacaataat tatttattct caatttcttt taacttcgct
     4081 ttatataggt ttctcaatgg gtgttgcacc aataataagt tataactatg gaagtgaaaa
     4141 taatacgcag ttaaatcatg tttttaagat ttgtatatta tttatttcag tagcatcttt
     4201 attggtattt atcttttcta tatcatgttc accaatcatt gtagagattt tttctcaaag
     4261 aggaagccga gtttatgaaa ttgcaaggtc tggattttat atattttctt ttagttttat
     4321 tttttgtggg tataatattt ttacttcgtc tatgtttaca gcattatcaa atggaaaggt
     4381 gtctgcaata ttatcttttc tacgtacatt tggatttata actataggtt tattgatatt
     4441 gccaaatata ttaaatataa atgggatatg gttagcagta ccaagtgctg agattttaac
     4501 tttattttta accatttttt gtatatgtaa gtataagaat agatataaat atatgtagat
     4561 attaaataaa tagtttaggt agatatttta tgttttaata agtaattcaa ttagtgggag
     4621 atatatacta ttgaaaatta aagttataat tgatataaag aattaataaa aataacatat
     4681 aaaagtttaa aagggagttg aacttaaagt ttgattagaa aaagtcaatt ttaagacaac
     4741 tccttttatt ttactattgt attaatctgt atctgtaagt gtattagtct aatattataa
     4801 actttggatg aaattaattt tctataggtt ttaatgtatc atacacttta ttttaaagaa
     4861 acttatctta attttcatag tgattgaatt tgtctattag agatgttact attagtaatg
     4921 taaaagcaag agtagtgaat ataaaaaaca agatagtgat actgcaagat tagaatataa
     4981 taaattttta tggagagatt tcaatatgga tacatgtttt aataaaaaaa tattacttgt
     5041 tgatgatgaa aaggatatag tagatttaat tgaagaagta ctcataaatg atggttttaa
     5101 aaacattatt aaagcttata atggtctaga tgctatttcg ctgtgtaaga tagcatgtcc
     5161 agatgttgtc attcttgata taatgttacc agatatagat ggtatagaag tgtgtaaaaa
     5221 aattcgagag ttttcatact gttctattct ttttttatca tcaaaaaatg atgatattga
     5281 caaaatacta ggtcttagta gtgggggaga tgactatata acaaagccat tcagtccaag
     5341 agaaattgct tttcgagtta aagcgcaatt acgtagacaa cagtatcaaa gcattgtacc
     5401 atcagatagt gaagttatta aaataggaga tattactatt gatatagaag gaaaccgtgt
     5461 atataaagat agaaatgaga ttgagttaac tggaagggag tatcatcttt taagctacat
     5521 ggctcaaaat gtaaataaaa ttataggaaa agaaagactc tatgaacaag tatggggagt
     5581 atatagtagt atttgtgaca atacaattat ggtgcatata cgacatataa gagaaaaaat
     5641 tgaagataac ccctcaaatc ctaaaatact tataacagtt aaaggactag gatataaact
     5701 tgtgaataga atcgattaga atagatttta atattaatga agatatctca agttgaattt
     5761 ttagttaatg aggcattctt ttatgggtga aatcaaatgt attttcgtta tctcaacaat
     5821 gaagaagagg tctatctcta ttttgagata gcctcattaa gtttatatcc tggtaatgta
     5881 ctttatgttc taataaaatt tttttagttt atttgcagtg aaacattaac tagaacatta
     5941 aaatttgcat tgaaatacca attaaataat attgtatatt gtgtataaac ttatactaat
     6001 tgtttttcta tattaaatac tttatcaatc caacctttat aaaatttttc ttcatgggaa
     6061 actaggatta tacttcctct aaactgtttc aaagcatttt gtaaagcatc ctttgtctct
     6121 gcatcaaagt gattggttgg ttcatccaga ataagaaaat tacaaggaga taacatcata
     6181 caacataatt taaccttaga ttgttcacca ccacttaaag tagatacact ccttgaaaca
     6241 tgctcttcct tcacaccaca tctggctaga tgacgtctaa tctcttttgt atttaatttt
     6301 ggatatttat ctgctactat ttgaagtggg gttttatcag gattttccca ttttaaatct
     6361 tgctcataat aaccaatttt aacttgttct gaaaactgga aatctccaga tatacgagga
     6421 atatctttga ctaatgtctt taacaaggta gatttaccga ttccattaaa accagttata
     6481 actatttttt gacctccatc aacagagaaa tttaattttg gaagtaaaga atagtagtat
     6541 ccaacttcaa gatttgttat agtaagagca ttttgagccg atatttcaat ttctgaaaat
     6601 tgaatatttg gttttcctgt aaaactagga ggagctattc tttctatttt atccaattgt
     6661 ttttgtcttc ctcttgcaat ttttgaattt actccagctt tatttttacg tataaatgtt
     6721 tcttcttttt ctattttctt ttgttgtgct tgataccttc taatataatc ttctcttaaa
     6781 tgtgattttt gtttaaggaa ctctgaatat tttccatgat atttcttaat catgccaaat
     6841 tctatatcaa gaatacctgt agaaattttt tctaaaaagt caaaatcgtg ggaaactaca
     6901 ataaatgccc cattaaatgt atttaaataa tttgagagcc attcaacatg ctctttatct
     6961 aaaaagtttg ttggttcgtc taataataga acatcatggt tagaaagtag taattttgct
     7021 aaaattacct ttgccctttg accaccacta agctctctaa caacacgatt cattcctatt
     7081 gaatctaatc caagaccatt tgctatttta tttatttcat tatctataga atagaaatta
     7141 cttgctatta gttgttcctg ataatctgat gctttgatta gatattgctc attttcagat
     7201 atagcacttt tttgatagag taaattcatt tccttttcta ttttataaag ttcttcaaat
     7261 gcagtgtgta aatagagtga tattgttgta tctctattga tttctgcata ttggtctaaa
     7321 tggcctatat tgatattagg ttgccacttg atttctcctg aatctggaac aacttctccc
     7381 aataaaattt taattaatgt acttttacct gtaccatttt ggccaactac acctatatgt
     7441 tcacctttga ataaatcaaa gttagctttc tcatacagtg atttatccat aaaacattgt
     7501 gataaattag ttacttttaa taaactcata attcaaactc cttttctttt attaaaaaat
     7561 ttatggaaat aaaaaaagca gaggaatggc attgtaaaat gccgacctct gcttttggaa
     7621 aaacaaaagg tcatagacaa gcattgttgt ctatgaccag aaaatctata ttatctatcg
     7681 gcaaataggt aattttaaat aagcatagcc aaaaaactat actaaaccta tatttgccga
     7741 tgaagataga aagctttaag ctttcagatt aacctctaca caacgcttca tcgtatacat
     7801 ttgtgtagag ttaagttttc taacaagttt aactaattct atataaatca taattattca
     7861 catcctaatc ttagtattaa ttctagttta tctgtattta aattgtactt ttgtactttg
     7921 tggattatct tatttattta aaatatgttt tattaattat agcttaagtt atgttatcat
     7981 aatacttagt tatagtcaat aagaaagtta tatttactat tattatatta atttagtaaa
     8041 tctatcatta caaagttatt tgaagactcg ctatcaataa aattttatag tctataagaa
     8101 aattaagatt atgagtttta taattttgat atagggatac tattttcata aatattacaa
     8161 catgtagaag tatatgtcta ttattgttga attttcaatg atat
//
LOCUS       DAELNY010000167         8071 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.169, whole genome shotgun sequence.
ACCESSION   DAELNY010000167 DAELNY010000000
VERSION     DAELNY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.169"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(73..543)
                     /locus_tag="KPE55_003029"
     CDS             complement(73..543)
                     /locus_tag="KPE55_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0494072.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1141..1530)
                     /locus_tag="KPE55_003030"
     CDS             complement(1141..1530)
                     /locus_tag="KPE55_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0494073.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3187..4593)
                     /locus_tag="KPE55_003031"
     CDS             complement(3187..4593)
                     /locus_tag="KPE55_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0494074.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4638..5345)
                     /locus_tag="KPE55_003032"
     CDS             complement(4638..5345)
                     /locus_tag="KPE55_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494075.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5516..6430
                     /locus_tag="KPE55_003033"
     CDS             5516..6430
                     /locus_tag="KPE55_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494076.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPEKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6430..7191
                     /locus_tag="KPE55_003034"
     CDS             6430..7191
                     /locus_tag="KPE55_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494077.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7203..7940
                     /locus_tag="KPE55_003035"
     CDS             7203..7940
                     /locus_tag="KPE55_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494078.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2721 a         1306 c          865 g         3179 t
ORIGIN      
        1 actagtccta tatattatct tgaattagtt ctattacaat taatttctac taaataatta
       61 taatttaaac aatcattcct tactattttc acactgcttt acaaatttaa ttggatgttg
      121 aattatttta gaagtttctt ctacagagta tccctcagat aaatatcttc tagctataac
      181 ttccatagac tctcctatct caataatatg cttatccggg tcataaatac gcactactct
      241 ttgttgccat tcatgtttta gtggcttatg gacaaactct acatctttat aataattgag
      301 tttttcaata aattcgtcaa aatcatcaac ctcaaaataa agttccatat tatttgattt
      361 ttgtataatt gtatctggat taatccctgt aagccatgcg aagtcttctt gtatagcaaa
      421 tcctccttta aatgttacat ttttacctaa gtccaattca atttcttgct caaataattc
      481 tttgtaaaat ttttttgata cttcaatatc cttcactgct aataatgaaa cctgatattt
      541 cataaatttt cctcctaatt ttttatttct attttttcag tatcttctat taacattttt
      601 ttctctatct cttcagtacc ttttattaat cctttatttt tactttttta gtatcttcta
      661 ttaacacttt tgtttctacc tcttcagtat cttatatcaa tcttttattt tttcaatatc
      721 ttctgttaac acttttatct ctatctcttt aatatcttat attaatcctt tatttttatt
      781 ttttcaatat catcattaat tcctatttat tttatcatct taaatacgaa aacactgtgt
      841 catatttgca aaatatatat tatcatctat attaaagctt cacaatttat atttaaaaat
      901 tctctttaat tacactacct ctatttaaaa agctacctta attatactta taagtaaagg
      961 tagcttttta aatttaatta aatagaatat ccactattaa tttatattta atttatttag
     1021 attcacatta tagtatcata atccacttat aatcatttgt catattactt aagtagacta
     1081 atatattaca taaatacact tacatacact ttatattgat gctataagaa atacgtttgc
     1141 ttatacgtct ttctcctctt cttttagatt ttttatccac tcttcaataa gtaaaatatc
     1201 atctttgctc aaattaatat tttcatgtaa tgacaacaat aaacttttta tagaagaatc
     1261 atgaatattt ctaaaaaaat ctcttgtctc aactcctatg tattcctttt ctttaattaa
     1321 aacttcataa tgcgtatatt tatctatctt ttgagaattt agaaatcttt ttactactaa
     1381 tctagataaa agagtcaaag ttgtcgtttg tttccattga tatttttgct ccattcctaa
     1441 aacaatatct ctagatgtta ctttggaatc tgatttccat ataaatttca ttatctttaa
     1501 ttctgattgt ggcaactttt ttatagtcat gataaaacct tgctactcag aatatagaca
     1561 agcaatatcc ataataaaat ttttcgatta aatacatctt atacggattt atataagctc
     1621 agacttataa gatatttttc aatcttaatc ctaaatttag ctatttgtac acggtaaata
     1681 tatataaata atgtcaaaat tctcttgtat tatattgctt aattttatga aatattttgt
     1741 actcagctat ctattttgga tacttgtcca gtagctctcc tttcttttaa actctttgat
     1801 tttaaatatt tgtatttcag taaatcctac ttcctaaatt ttttatttac tacaacttat
     1861 tactgaactt cctacttcct aaacttttta tttactacaa cttattactg aacttctcac
     1921 ttactaaatt tcttacttac tataaattat tactaaactt cctactttct aaatttctta
     1981 cttactataa attattacta aacttcctac tttctaaatt tcttacttac tataaattat
     2041 tactactaaa cttcccactt actaaatttc ttacttacta taaattatta ctaaacttcc
     2101 tactttctaa atttcttact taccacaaat tattactaaa cttcctactc cctaaatttc
     2161 ttacttgcta taaattatta ctaaacttcc tactttctaa atttcttact tgctacaaat
     2221 tattactaaa tttcttactt gctataaatt attactaata aacttcccac ttcctaagtt
     2281 tcttacttac tactacttat aactaaactt cccacttcct aaatttctta cttactacca
     2341 cttataacta aacttcccac ttcctaaatt tcttacttac taccacttat cactaaactt
     2401 cccacttcct aaatttctta cttactacca cttatcacta aacttcctac tttctaaatt
     2461 tcttgcttct taagaataat gttaatttta attttaagat tatagttttt accttgttta
     2521 attgaaacat tctatataat tatatccttc tattttagag tttttttatt tatatattac
     2581 aaagtttaaa ttttttcatt aattatatta taaattttcc atatatttca acataaatac
     2641 atatatatat atgttatttc taaaaatact acacaaaaac tctactttag tagaacttaa
     2701 tggcgcaaaa tacttcaatt taaaacattt atcgtacttt aaataaataa ctataataat
     2761 atacttacaa tagttacata gaagctatta taactacaca gaacagcgta taattaaaac
     2821 ttttttagga tgtcattata ttttttctta aaattaaaaa acactatacc ataattctat
     2881 tttagtagaa tttttttgtc aataataaaa ttaaatatta actaaaaaat aaaaaaaatg
     2941 gaattatcta aagaatttaa taaccttaca ataattccaa aatattattt ctatgaaaat
     3001 tttgatacca tgagtaactc agccataagc gcataaagca caagttactc tttactttaa
     3061 taaaacttga caagaaaaga atttatcctt attctactct aatagagtta atttgtcaat
     3121 aaagaataaa attattttca aaatattttc attcttcatt cttatctgtt attattttat
     3181 aaaatatcac ttaattagag gtaattttag tattgttttt agtccctgac tatcaatgct
     3241 cgtaatatct aatttacctc tatgtgcatt aataatgcca gatactatca aaatacctag
     3301 accatgattt tgacctttgg catatgcctc acttagtcca ttttggccct ttaataaact
     3361 attaattctt tcttgactca taccttttcc atcatcttca acaattatat agcaaaaatc
     3421 taaatcagac ttagaggtaa cccttatatt acatccttgt tggttatgag ttatactatt
     3481 ttgtattaaa ttactaatag ccctttttaa taatttttta tctgcatcta taataatatc
     3541 ttcatttagt atatcaagtt caacgtaaaa gtcattatcc aaattattat ttataaactc
     3601 agaaataagc tctctcaaaa acgcagaaac tctaatctta tcacaattca atgtttgcat
     3661 attatattca agtttagaca ctaaattgag gtcatttact aattctctga ttttaatccc
     3721 ttgattgcat ataatagaca cttgctcttg ttctctattt gaaaggctac tgctttcttc
     3781 aagttctcca gcatatccaa ttatcattga aagtggagtt cttatatcat gagatattcc
     3841 tgcaatccag ttagacctag cttcctcttt cttcctaagt ttttcatctt tcatttgcaa
     3901 ctctttagat acagtattaa tagattttga aatatcactt agtattcctt tttcttgtaa
     3961 aagtactggt tctccttttg acataagctt tatgccagta ataattggtt ttatagattt
     4021 caccattttt aaacctatga atagagctat taaaaaagta actattgcat taaccagaaa
     4081 catacctatt attaacatag gaaattgttc tatttcctta accgtataag taaggttaaa
     4141 cttagtgtac ttgtcttttg gataacctgt tactaatatt ccatttggat gttcccatac
     4201 atacacagga tagtccttta aaaagaacct tgaaaacttt gctacatctg tcaaagtata
     4261 atgacttgga atttcttttg gtaaatcaaa tccccatatt ctatctccat tgttatcaat
     4321 taacattgcc caaactttat ttttatgtag tacttcctta ctagaatcat ctaaaataaa
     4381 ctttccattt cttaaaatca aattatcttc aatcataatc aagtttgatt ttatttctct
     4441 gctatcactt gtatctaaat tatcagagta tttagataca aatagcatat atgaaaacag
     4501 aagaaaacct gctatattta atacaataaa aagtaatgaa atcaagatac ttgaacctat
     4561 aaatcgcctc aatatgtact ttacactatc caaaattaaa tcactcctct tgatttcttt
     4621 tattctaata tctaaaatta attaatcatc aacttatatc caagacctct tactgtaata
     4681 agatgtttag gtgtagatgg agttttttct atcttctctc taactcttct aacatgaacc
     4741 ataagggtat tttcatatcc atacatatca tctccccaaa tggcttgaca tagtgaatca
     4801 ttagtaacaa ttctcccttt attttcatac aactttaaaa gcaacgaatg ctctttagct
     4861 gttaaagaaa tctcttgccc atctttctca acacatgcac tatttaaatt cacaacaaca
     4921 gaatctccca gtttaaatac tggcaactcc tcttcttcta taggcacata tacacgattt
     4981 aatataccca caagccttaa agtaagttcc tttggtaaaa atggctttac tatataatcg
     5041 tcagccccta agcccaaacc aatcaatcta tcttcatctt cacctcttgc agagagaaaa
     5101 atcactggta ttttagatgt ctctctaatt tttctcataa tagaaaaacc atctccatct
     5161 ggcaatacaa catctaaaat tgcacaatct ggctttacat ttctaaatac cctaacagct
     5221 tcctcataac ttgatgctaa aaatactcta ctaaacccct cttttctcag gatttcatct
     5281 atcattttta ataattcctt gtggtcatca accacaagta tttttttatc ttttattgac
     5341 tccataatca tcaccctcct ttagtatacc atatacccat tttcacaact ttgcctaaat
     5401 agacttttaa ggttattgta aggtaaattt cagctaattg aaatgtaacc aagttattat
     5461 actgatataa gttaaaagaa tcaataagca cagattaaaa aaggggatga aacatatgga
     5521 aatgataatt agaacacaaa gactttcaaa ggaatacaat aaaatattta gagtaaagga
     5581 tatagaccta agagttccac aaggagctgt atatggtttt ttgggtccta atggagctgg
     5641 aaaatctacc actcttaaaa tgcttcttgg acttgctaaa cccacacaag gaaatattaa
     5701 tattttaggt aaagagttaa atgaaaaaaa tagaatatca attttaaaag atataggttc
     5761 tttgatagaa tctccatcat actatggtca tcttacaggg cttgaaaaca tgaccataat
     5821 gcaaagactc ttagatttac ctgaaaaaaa tgtaaatgaa gcattaaaaa ttgtaaggtt
     5881 agaaaatcaa aaaaataaaa aagttagtca atattcttta ggtatgaagc aacgtcttgg
     5941 cattgctatg gcaataatga agtttcccaa attattaata cttgatgaac ctacaaatgg
     6001 actcgaccca gcagggattg aggaaattag agaattaata aaatctctac cagaaaaata
     6061 cgggatgact gttctaatct ctagccatct tttaagtgaa attgaccaaa ttgccacatc
     6121 aataggaata ataaatcatg gcgaactggt ttttcaagat agcataaaag aacttcataa
     6181 tagaggtcaa agtcagattg ctatcaaaac taagaatgtt gtatctgcac aaaaactatt
     6241 aaatactaaa ggatatatgt cacttataca agatgggtac ttgactattg aaaatttaaa
     6301 agactcagat gtggctaaaa taaatggtat gcttgtccat tcaaatatag atgtatttag
     6361 aatagaagaa cataaaaaaa gcttggaaga tatattctta gaacttactg gtaaggcggt
     6421 gactttataa tgaaatacct tggtctagaa ttttataaac ttaaaagaaa gaagattatt
     6481 ctgatgacct ttttatttgt agctgttgaa ataatgtggt gtatcataaa ttcaaataga
     6541 gctattacac gtaatccaga tatgttaggt ggatttgaat attcctatat gcttatgagt
     6601 tttgcaagtt taaatggtct attctttcct atcttaatat caataattac atcacgtata
     6661 agcgatatag aacataaagg agatacatgg aagcttttaa aatcttctgt gacatcttta
     6721 aatagtattt atttatctaa atttttatgt tctgctattt tggtatctat acctgtattg
     6781 atgcaagtgc tatctattgt gttgtttgga cattttagag gggttattga gtcaatttct
     6841 gtatctttac ttttaaaata cacattagga acaattcttg taagtattgc tatcattgca
     6901 ctacacctat ggattgcaac tgtaattcca aatcaaatga ttgcaattac atttggcatg
     6961 attggtagtt ttataggttt gacatcaggt ttgttttatc aaggtattcg tagattgttt
     7021 atatggtctt attacctaga gttatcacct ttaagttatg cttatgataa tgttttaggt
     7081 tcatctgtct atagaattga tatgaatttt tctattacga ttttagtttt cttaattgga
     7141 atgctaattt attatattgg aagaaatcat ttattaaaaa aagaagtata ggagggattt
     7201 aaatgttaag gaagtcagtt ttgggagaaa ttctaaagtt aaaaaattct tttatatggt
     7261 atgtattatt agctttgcca cttataagtg tgttgattgg ttctggtaat ttttatctaa
     7321 atcaaggcat actcaaaaaa gaatggtaca gcttatggac tcaagttagt cttttttatg
     7381 gggaattttt ctttcctatc ttaatcgcaa tcttttgtgc atatatttgt aggcttgaac
     7441 atatgaatca taattggaat aatgtaatga cactgccagt taatttaaaa aatatctttt
     7501 tatctaaact tacagtagtt tcaatactta cagctattac acaagttttc tttgttatct
     7561 tttatatagt tgcaggaaat atgtttggtt tttctggtag tgtacctaaa gaattgactg
     7621 gttggattgc taaaggttgg attatttcta tttcaatagc ttctatacaa ttatacatgt
     7681 ctataaagat tagaagtttt gcaactccta ttggtattag tctttgcctt tctctatttg
     7741 gtatgggatt aatagtagca aaacctgctg ttggtatatt tttcccttat tctatgttag
     7801 gtagtgctat gggggttata aagcaatctg ctttaaatgt taatgacact atagttacaa
     7861 tatgtgttgc atttattact actatagtat tttctgaaat tgccaataga aattttagaa
     7921 agaatgatgt tatttcttaa tggtaaatgc tagatatttt ggggtttgaa atttatattc
     7981 tttttagttg aaatctatat ccattttatt taaaaatata tatcttttat ttgaatttta
     8041 tatgttttat ctactcgcta tataatagat a
//
LOCUS       DAELNY010000168         8021 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.171, whole genome shotgun sequence.
ACCESSION   DAELNY010000168 DAELNY010000000
VERSION     DAELNY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.171"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            456..2690
                     /locus_tag="KPE55_003036"
     CDS             456..2690
                     /locus_tag="KPE55_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0494079.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2721..3554
                     /locus_tag="KPE55_003037"
     CDS             2721..3554
                     /locus_tag="KPE55_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0494080.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3794..5527
                     /locus_tag="KPE55_003038"
     CDS             3794..5527
                     /locus_tag="KPE55_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0494081.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5919..6213
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5988..6016
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6743..6847)
                     /locus_tag="KPE55_003039"
     CDS             complement(6743..6847)
                     /locus_tag="KPE55_003039"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG0494082.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7472..7717
                     /locus_tag="KPE55_003040"
     CDS             7472..7717
                     /locus_tag="KPE55_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494083.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
BASE COUNT         3240 a          944 c         1053 g         2784 t
ORIGIN      
        1 taacaaaaaa tacaagaata aacttaattt taataactaa aattaattat gcaaaattat
       61 taatatggta ttaaattatg tataaaaagt taattatgaa taaaataatt atgcctttga
      121 aaaatcatta aacttaaaat taattataaa aaaactctac cttactaact atacagctca
      181 caatctatct atttatgaaa aaatatgata taattgcatt caaaaaggaa atctttcaat
      241 atactagaat ttaagcatta actaatttta tctaatatta ttactaaact aaaaaatatt
      301 tgtatttgta gcattggagc aagtaactta atgtatattc attttaaata cttatttaaa
      361 atttagcatt taaattattt acaaattaac atttaaatac ttctagcata aatacagttg
      421 tcattaaaat ttaaagtatc ttatggagga ataccatgca aaatcataat tatataaaaa
      481 tgttaaataa aaaggtagaa gtattatcta aggaattaga tagtagaaat tttaaattaa
      541 aaaccttaaa tgaaatagat tttttttcta aaataccaaa cagagaattt atgtaccaac
      601 atattgatga ctgtatttcg aaaaatatac atataggaat catgttatta aatatcgata
      661 actttaaatt atttaatgat atatatggat acctagtcgg agaaaattta ttaattactc
      721 taactcaaaa cctattgcac tttattccag aatcaaaggg aatagtcgct tatttaaaag
      781 gagaaggttt tttaattgta cttccaaatg tatcaaataa agagttaaat aatatgggtg
      841 caactattat agagagcgcc cgagaattaa aaataaaact tcctaatgaa aaagaaatat
      901 actccaatgt tacatttagt atcggcagca ctatctggaa tggtgaacca aacatgacta
      961 ctttgatgct tttcaaccaa gtatatagtg ctttaaaaaa ggctaaaagt gaaggtaaaa
     1021 atagatacat tctatttgat tttaaagata atagttttta caataaatta gaaaatgata
     1081 aggagttctt atcagaaata tcacatgttc tacaaactaa acaaattgaa gtattttacc
     1141 aaccacttta taatataaaa aataatacta tagttggatg tgaggcactt gcaagatgga
     1201 gacaccctat taaaggactt cttactccag ataaatttat tcctttattt gaacagtttg
     1261 gtctgataac ggaattagat ttgcatttat ttgaagagaa ctgtaaaaat ataagaagat
     1321 ggattgataa caatgatgtt tttgttccaa tttgttgtaa cttttctcgt cttcattttt
     1381 ttaaccctaa ttttccaagt atgctaaaag gtattactga aaagtacagc gtctctacat
     1441 caaattttag cattgaaata acagaaaata tattaataga aaatacaaat acaattattc
     1501 aacaactagc tgaacttcgt tcttatggtt tttctgtatc aatggacgat tttggttctg
     1561 gatattcatc ttttggtatg ttgcaaaatc taccttttga tataatcaaa attgatagga
     1621 tatttttatc tcataattta aatgatttta aaaatacaac cattttgtac tctataataa
     1681 acattgcaag agctcttggg atgcttacag tatgtgaagg tgttgaaaca gaacaacatg
     1741 tagaatttat taaaagtata aattgtgata tcgcccaagg ttattattat gcaaaaccca
     1801 tggagtgcaa agaatttgaa aaattactat ctaaagagaa taattcagag aaaatgttta
     1861 taaaatattc tgatatagga aaatctttaa tagagaagat atttgatgga ttttttatta
     1921 tgcaagatat gaaaaaacta agagctaatg tcactgaaga cattcaatgg tttgattttt
     1981 acaacaaaac tgaaattaca tcttttccta aagtaataga gcattttgaa aaacatatta
     2041 aaggtaaaaa attttctatt ctttacaaaa gtataacccc acataatact gaatcagatt
     2101 cattattaat tagtggagaa gccgttttat cagatgaagc ttctgaacat caaaaatata
     2161 gcaatttcta ctttagtgca aattgtataa tttcagaaaa taaattactc ttagcaaaat
     2221 tacatttgga tttaattaaa actgctggct atataaatga atttgaatca tattcaatac
     2281 ctaaaatgac aaatacacat cttgatgaaa gtaatatttt atcacaattt tattcaatgt
     2341 tgcctgtagg tataattcgc tacgatttat ttggtgatat gattattact tatatgaatg
     2401 aagaaatgtt tgatattata ggttatacaa aagaacagtt ttttggtgag atgggcggaa
     2461 atttaagagc aattgttcat ccagatgatt tagactttgt ttataaaaaa agtttggata
     2521 tgattgacac aaataaaatt gaaccatttt tatatagatt cataaaacga gatggtcaaa
     2581 ttgtcacagt tatgtataca caatgtaacc tttctgcaat tgatggaagg tcgattgccc
     2641 agggtatgta tgtagatgta aataaaataa aaaatttaac aaaaatctag tacaaagtat
     2701 tttattttgg agaaattaat atgtttaaag aaattttttt aagaactttt ccaggatttc
     2761 taattataag agattctaac tacagaatta tatttataaa tgataattta aaaaatttaa
     2821 ttaaaagtta tatgcaagat aatccacttg gaatgactaa tatagaaata gctaagaaac
     2881 tccctgataa tattgctaaa ttttttacgg attctcacaa tattcaatta gattgggaaa
     2941 aaaattatcc ttatgataaa atttcaaatt ggattttaga gtttaaaaaa gatactacaa
     3001 gctattggaa tgttttagaa tataaagtag atgttgatga aaaaacctac ataattacta
     3061 tggccaatga tataacaaaa ctctatgaag aaaataagag aaatcttcac tactcaatta
     3121 ctgacccatt aactggagca tataatagaa aatatttaaa tgatagattt gacatattta
     3181 taggagatta tattgtacta attgacttag ataatttcaa aatgataaat gactatgaag
     3241 gtcataatgt aggagacaag attttatgtg attttgtttc acttttaaat aaagaattaa
     3301 taaattctac ttctattata agattaggag gagatgaatt tattgttata ttctctagtg
     3361 atgttgacaa aaattatgtt tattcacaac tagaagcctt aagagaaaat tttttaaaag
     3421 tattctctaa gtataagtat ctttcattta gttatggtgt tgatacagta aaaagaaatt
     3481 taaaattgac aatagttgaa ttggataaaa aaatgtataa aaacaagaaa aaatttgata
     3541 aaaatgatta ttaatattgt tattttaaaa taaattaaaa ttatctttct ttttttaaac
     3601 ttgtacatta gtttataaat atgtatctat acaagatatt gacattaagt actgaagcat
     3661 atattgtttc agttcttttt tgttaaaatt aacttattta acaaagatat catagtttaa
     3721 caaagctttt aatttaaata tattgatgta taatataatt aaaatataat agcaaaaaga
     3781 aaggaggaat atcatgagca tactcctaaa aaaagcaccc aaattagcaa aacatattat
     3841 aacaagtttt tatataaatc gggatattga tgaagtatta aaataccttt gtgaaaatgt
     3901 tacatggatc ggaccaggag aacaggaatt tctcacttct tttaatgaaa taaaaaatta
     3961 tttttatgca ggtcaagatg aaattccttc ttgtgatata aataatgata tatttgaaac
     4021 tgtaagtgaa gatgagaata gatgtatggt attaggaaga tatactgttc gaactaaaga
     4081 aaatactcaa atgattttag aggtaaatca acgttgtaca tttgagataa ttgaggatag
     4141 aggaaaatta ttagtaaagc acatgcacat atcaaaccct tatggtgaaa tgcaacttga
     4201 tgaatacttt cccactaaaa ttggaactca aagttatgat tatttgcaaa gattactaaa
     4261 ggaaaaaaca gaagttattg aaatgattac aaataatatc aatggtggct taaaaggtag
     4321 caatgatgat aatacatact catttttcta tgtcaatgaa ggattgccca aaatattagg
     4381 atacacatat gatgagttca tggaaatgag tggtggttca gcagtgggtg ctgtatatcc
     4441 accagactta cctaaagcac tagaagattg tgaacgatgt tttgcaaaag gacctacata
     4501 ttcatctgaa tatagaatcc gaaaaaaaga tggaacatta atgtgggtat tagattctgg
     4561 aatgaaatca ttaaatagtg atggcatagt aaaaattaat agtattatta ctgacataac
     4621 ccaactaaaa aacatagaat ctgaactaaa attagaaaga gagcgatatc gtattgcttt
     4681 gcaaaatatt acagatatca tgtttgagta tgatattgaa aacgataccc ttataaaata
     4741 tcaacgtgta gaaattgaca aaaaaattga attagaaaat tttgaaacaa aaaattactc
     4801 caaactatta gaatctggta aaattattca ccttgatgat attggaaaac tactagaagt
     4861 acttcatgga aatcttcgtg aaacaattga gataagacaa gtaaattcat taactaaaaa
     4921 tgaatggaga tggattcgtg ttcaatgttc agttatatat gatagtgacc ataaccctat
     4981 caaaacaata ggcgttttaa aagacataac tgaagataaa tctaaattag aaagcttaat
     5041 taatcaggct caacgtgacc cactaacaca attatataat caaagagtat cacaaaatct
     5101 tattgaagag tatctatgta gttctgattc caaaaataat gatgctctat tagttattga
     5161 tattgatgat tttaaaactg taaatgatac ttttgggcat ttagagggaa atgaagtttt
     5221 agttgctgta tcaaaaatac tattacataa cacacatgat aaagacatag ttgctcgaat
     5281 tggtggagat gaatttacaa tatttataaa atcattaaca aaggatttaa taataaaaat
     5341 tactaatgat attttaaatg atgcaagtaa aataaaagta aaggacaatc ataaaataac
     5401 attaagtatt ggtattgcct tcactgatga ttcaactaaa ttatataaag atttattttc
     5461 taaagctgat aaagctcttt atctatcaaa agcagatggc aaaaattgtt atagtacata
     5521 cgaataataa agctatttat acgaccaaga atgaaccttt aaatttaatt tcatagtcta
     5581 acaatcaaag agttgaagct aatcttatag ctagtcaact agttcctaga aaaaatttaa
     5641 acaatatatt tattgatact gctcattttt ctaaagagca aatttatata aaaaattaaa
     5701 cttttatgta catacagata ctaatgataa acagttatca ttagtatctt ctatcttaaa
     5761 acaaaatgat aatttatcta tataaaaaag tgctttgttg acagagttca cttttttata
     5821 taggtacaga atttaagtat aaatacatgt tttattctat acattttagt taatgtaaaa
     5881 tttcttgatt gtactctcta acttttcaac attatactat ttatatacca cttagttaat
     5941 ataaacccct tagctggttc tagtgctttt tctatgattt tagtcatatt tacataccac
     6001 ttagttaata taaaaccaat ttagatgatg tatatgtgca aaaggaagaa ataatttaca
     6061 taccacttag ttaatataaa acggtcacta caacttatac ttccagtcta acgatttgaa
     6121 tttacatacc acttagttaa tataaaacat attttggatt tattaatata aacataatta
     6181 aaaaatttac ataccactta gttaatataa aaccccaaaa taaactgggc attttcaata
     6241 cttacacata tgcaactctc ttaaatttgc agtgaaccat gagtagtgca aatgataaag
     6301 cttatcatat accctcaaca ccttatattt caagcgttaa gctctacttt gtaacaaata
     6361 tcgctcactg caaaactatt tacattttta ttatatcata aatatattat ttttgaatat
     6421 ctataccaat ttgtggtata ataaaagcaa gaagaactac aatctattta gacgtagagt
     6481 gaagttcaaa tactaatgtt tattcttttt gaacttgatt tttagttcaa aactaaagtc
     6541 actcctgcct gtgtgaacta tacccgctgt tttatacagt taaaacatgt aaaaatagtt
     6601 ataaagtact agtgtaactc attagtactc tttttattat attcatataa ttaatatcta
     6661 tattttaaag tttactatct aaaaaagagc acgtcaaata aaaccctaga tatttaattt
     6721 ttttagagat acttaaaata aattatgctc tctctgtact taaatatttc ctaaattcaa
     6781 atggagacat agaatttaat ctattttgat atctaaaatt attatagtag tatatataat
     6841 catctattgc tttaactaat tattcatatg tatcaaattt ccttaaatag tacatttcta
     6901 cctttaaaat tccccaaaat ccttccatag gagcattgtc aaggcaacga ccaactcttg
     6961 tataatatat gtatatctaa caccattaag aaatatatat tttataatat atatttcttc
     7021 atttaatgca cttagaaaag caaagaaaat acagattagt acacttttta gagaaaaata
     7081 aaattaatac atctaaaata ttaaaaaata ctttagataa tctaaagaca catctcaaag
     7141 tttttaacta aattataaaa ctaattacaa atgctatgta acgataataa ataaaaaatt
     7201 tacttcgctt aagcgacgtg tcgttgaaat atttcatgtt gccaactggt tcgtaagttg
     7261 ataagaaaag aacactaatt atttagagtt cttttcttag ctacaagtgg attataatac
     7321 ttttaaatct gtgagaaaat catatataat aatgatatga acaagtaaag aaaatacaaa
     7381 aatgacaaaa ttataaataa aatattataa aatcataagg tcataattgt tatattgtag
     7441 aaacaaagta aagagagagg gtggagaata aatgaatcaa aaaaaaataa agattagtat
     7501 taaaatggta attataggta tatttgctgt aattattgct tttgtattat ccagattatt
     7561 cgttgagtat attgatgaat tattaaggtc taattggcaa tatcagctat ttgaaagtga
     7621 gagttcatat atagtatttg ccaattgtat tatgttctcg aatatattat tagaaatatt
     7681 tttaatatat atttgtagaa aatttagaaa aatctaatat ggtcaacgta tttcgaatcg
     7741 aatacttcat gattatttag tctattgtgc tagttaaagt ttaattccca tataaagtaa
     7801 attgatttta gtaaaacaaa tcacccctaa gggttagttt atggataagt catccaaata
     7861 ttaactcttt aatttttgct atagattcat ctttgctcat aactttactt ataaaaaatg
     7921 aaatattata agctagaact tttattctaa ttctagtcat aaatcctagc atagagttac
     7981 tttttttata ttataaataa aactatacaa caaagataat a
//
LOCUS       DAELNY010000169         8015 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.172, whole genome shotgun sequence.
ACCESSION   DAELNY010000169 DAELNY010000000
VERSION     DAELNY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.172"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..1831
                     /locus_tag="KPE55_003041"
     CDS             35..1831
                     /locus_tag="KPE55_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG0494084.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENLQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(1971..3752)
                     /locus_tag="KPE55_003042"
     CDS             complement(1971..3752)
                     /locus_tag="KPE55_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0494085.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(3775..5661)
                     /locus_tag="KPE55_003043"
     CDS             complement(3775..5661)
                     /locus_tag="KPE55_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG0494086.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(5678..6175)
                     /locus_tag="KPE55_003044"
     CDS             complement(5678..6175)
                     /locus_tag="KPE55_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG0494087.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(6493..7974)
                     /gene="cls"
                     /locus_tag="KPE55_003045"
     CDS             complement(6493..7974)
                     /gene="cls"
                     /locus_tag="KPE55_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0494088.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         2770 a         1346 c         1140 g         2759 t
ORIGIN      
        1 gtttcattat ataacaagaa agttggaatt ttttatggga atagaatatt ttagtaaact
       61 tttagaaagt agtactgaaa aagaattgct cttaagtaaa aagtcaaata atataagtac
      121 agcaagatta atatcttttt tgatagtgat agcagggctt gctattggat tttacaataa
      181 aaatatagta ggtatttttg ttggagtttt atcaataata atatttatag cactcttagt
      241 tattcatagt aaagtaaaag aagaagaaac atattttaaa agcaagagcg aagtattaaa
      301 taagtatatt aagagatttg gagatggttg gaaggaattt aaaatagatg gaaaagaata
      361 cttaaaagat gaaaacttgc aagcaaaaga tttagactta tttggaagag catcattgta
      421 tcaatatatc tgtgtagcta atacatctta tgggaaaaat tttttagcca aatatttgtg
      481 gaatgaaaat ccagatgaga atgtaatttt agaaagacag agagctataa aagaactttt
      541 atcaaaagat gatttttcta ttcatattca aacactcagt aatataattg gtaaagagca
      601 aaaaaataat tcagatagta gtatagaatc gtttatagag tatggagaaa ataagaatgt
      661 gtatatacca aaatggatgc atatttttac ttggggttta ccaactgcaa caattttatc
      721 ttttatattt tgtatgttag gatttttacc tgttttacct gtttttttac tttttataat
      781 ccagttaggg tttagtggtt ttggatatcc aagacttatg caaactcttg ctccattatt
      841 ttcatttagt agaagtatac aagtatatga aaaaatgttt gaggttttag aaaaagagac
      901 gtttgagagt gcatatttga aagaattaca agataaactt tctaagggaa gtggagtttc
      961 aagaggtgtt aaacaattaa attcaatagg aaatgctgta aacctaaggt ataatcagat
     1021 actatatata attgcctgtg gtgttttgat gtggaactat cattgtgcag aagctttgga
     1081 gcgatggaaa ggtatatatg gaaatcaaat tagagattgg tttgaatcta ttggagaatt
     1141 tgaagctcta ataagtttaa cagttatttc tcatgtaaag gaaaatacat gttttccagt
     1201 aataaaatat gaagatactc caagattgaa agttgaagag gtataccacc cattaatagc
     1261 agaaaaaagt gtcatagcaa attcgataca attaaattct cagacttgta ttatcacagg
     1321 ttcaaatatg tctggaaaga caacattttt aagaagtatc ggtgtaaact tagtgctggc
     1381 atatgcaggt gctccagtat gcgctaagag ttttgatgcg acctgtatgg ctatatttac
     1441 ttctatgaga atacaagatg atgtaagtca aggaatatca actttctatg ctgagattct
     1501 tagaataaaa tcaatgattc aatatagttt aaaagaactg ccaatgttgg ttctagtaga
     1561 tgaaattttt aagggaacta actctgctga tagaattatt ggagcaagtg aggcagtaaa
     1621 gaaattatca aaaccctggg ttataagtat ggtgacaaca catgactttg agctatgtga
     1681 cctttcaggt agtggagatg ttgaagttgt aaactatcat ttctcagagt actatgtaga
     1741 cgataaaatt cattttgact ataagataaa agatggaaga tgtaaaacta caaatgcaaa
     1801 acaacttatg agaatggcag gtattcttta attaatagat aggttaatta aagaataagt
     1861 ctatttagaa atataattta atttgtgaaa ttaataagga ttgtcttaaa ttacgtaaaa
     1921 gtattttaag acaatccttt tttaagaata aattttatat tttgttatga ctacttagct
     1981 tcttcacaag cacattcttg tggacactct ccatagacac aactcttatc aaagtaatga
     2041 gtatgaaggt atttatgggc tacatcacta tttggttctc caaagaagtc tttataaata
     2101 gcttgtaaat caggattctc atgagattta cgaatagcct tatttctatc tatttcatat
     2161 aacgcatttg cacgtctatc aactatttca gtattaccat ggtgataagg ttgaccagca
     2221 cctccaacac atccaccagg acatgccata acttctatga tatggtattt acattctcca
     2281 cttctgacct tatccataat ctttctagcg tttttaagtg tatttacaat acacacatta
     2341 actgtagttc catcaacatc tatacttgct tccctaattc ctttaagacc tctaacatta
     2401 gtgaagttta cattatctaa agtcttatta gttatcttct catatgaagt acgtagggca
     2461 gcttctaaaa ctccaccact tgctccaaat atagatgcag cacctgtaga ttttcctaga
     2521 ggattatcaa aatcttggtc ttgaagatta ggtaaatcga ttgcagcttc ttttatcatc
     2581 ttagctaact ctctagtagt tatagaaagg tcaacatcta gtattccaga ttcacttaat
     2641 tcttctcttg aagcttcata ttttttagct acacaaggca ttacagatac aacataaagt
     2701 tcttcaggat tgattcctaa tattttaggt gcatagtagt ttttagctat agaaccaaac
     2761 ataccttgag gagacttaca gctagatgct aagtttaact tgtctggata attgtgctct
     2821 aagaagttta cccatgcagg acaacaactt gttaatatag gaagattttc acctttttgg
     2881 attctttcta taaattcagt agcttcttcc attatagtaa aatctgcacc aaagtttgta
     2941 tcaaatacat gttcaaatcc aagtgctttt aaagcagcta ccattttccc tgtagagatt
     3001 gaccctggtt ctaatccaaa ctcttcacca atagcaactc ttacagcagg agcaacttgc
     3061 actactatag tcttttcttt tttatttaaa acatcccaaa gtttaggtac attatctact
     3121 tctgttaatg ctccagttgg acagacagat atacattgac cacagaatgt acactctgtt
     3181 tcaaccatat cagagttaaa gaatgtacta actaaagtat taaatccacg attaactcca
     3241 gataatgctc ctactgtttg tatgtcatta catacagtct cacatcttct acataatata
     3301 cacttagaat ggtctttaac taaagattta gtagaagtat ctttacctgc aaaagattta
     3361 gaaccttgat atcttatttt tcttactcct aaatcagcag ctatagtctg taattcacaa
     3421 tctccatttt tttcacagac aaaacaatct tgtggatggt ctgataacaa taattcaaca
     3481 atagttctac gagcatttaa tgctttagca ctattagtct gaactttcat accttccttt
     3541 ataactgttc cacaagctgg aactaatcct ctctctgtct caaccataca aacacgacaa
     3601 gaagcacaag tgtcaagctt atctatttca ttcatatgta aatgacaaag gttgtgtatt
     3661 tttatattta ttaatttcgc agcatctaag atagaagttc cactaggtgc tgaaacgtgt
     3721 ttgccattaa tagttaaatt aactaaactc attgttattc ctcctccaaa taagttattt
     3781 cttgattata gcgttaaatg tacaattatc tatacaagca ccacatttta gacacttaga
     3841 agtatctatt gtatgttgtt ccttaacaga acctgttata caaccagcag gacacactct
     3901 agaacatttt gtacaaccct tacagtcatc tgttataaag taagaaagta agttctgaca
     3961 tttaccagca ggacatttct tatctacaac atgagctata tactcatctt taaaatattg
     4021 aagagtactt aaaacagggt ttggagcagc tttaccaaga ccacataatg aagcagtttg
     4081 tatagtttca gcaaggtttt ctaggtcatc taaatcttgt agagtaccat taccttctgt
     4141 aattttagtt aatatttcta ataatcttgt agtacctata cgacaaggtg tacatttacc
     4201 acaagattct tcaacagtaa actcaaggaa gaacttggct atatctacca tacagtcaga
     4261 ttcatcaaga acaagcatac caccagagcc catcattgaa ccgatagaac ttaatgaacc
     4321 aaagtctata ggagtgtcta aatgttcagt tggtatacat ccaccagatg gtccacctgt
     4381 ttgaacagcc ttaaatttct taccttcagg gattccccca ccaatttcaa atacaatatc
     4441 tcttaaagta gtacccattg gaacttcaac aagtcctaca ttttctactt ttccaccaag
     4501 agcaaagact ttagtaccac tagaatcttc agtacctaag ttggcaaacc aatctccacc
     4561 cttaagtata attgcaggta tgttagcaaa agtctcaacg ttatttaagc aagtaggaga
     4621 tttccataga ccactcttag aagaactata agttttcatt ctaggttcac cacgtctacc
     4681 ttcaattgaa tgcattagcg ctgtaccttc accacaaacg aaagcacctg caccatattt
     4741 taattctaat ttaaagttaa atccagtgcc taagatattt tcgccaagta aaccgtattt
     4801 ttcagcttga gctatggcta cttttaatct ttctatagat tttggatatt ctgctcttat
     4861 gtatatgtat cctgtatcag aacctatagc gtatccacat attgccatag cttctagtac
     4921 actatgaggg tcaccttcaa gtacagatct atccataaat gcacctgggt caccttcatc
     4981 agcattacat acaacaaact ttttatctgt tggaccagct ggattttttg aagcagcttc
     5041 ccatttagag ccagtaggga aacctgcacc acctctacca cgaaggccag aagtttttac
     5101 ctctgcaata acttcttgag gagtaagaga agttaaacat ttgcctagag ctaaataacc
     5161 atcattggca atatattctt ctatactatc tgggtctata agaccacaat tttttaaagc
     5221 tatacgtaac tgttttttgt agaaagacat ttcttcttgt tcttcaactt ttttacttaa
     5281 agattcttcc tcatataata attcctcgac aactgtattt ctaattaagt ggctttgaac
     5341 tatcttttca gcatcagatg gctcaacttt tacgtagaaa acgttatcag gataaacttt
     5401 tacaatagga ccttgagcac agaaaccaaa acaacctgtc aaacgaacac taactttatc
     5461 ttggattcct gatttcttta tttcattttc taattcacta actatttcta gactgtttga
     5521 agatgtacat ccagtatcac aacaaacaag taattctctt cttaatacat tttccttaga
     5581 ataatttttt cttaagctaa gattaggttt cagctcatca gctaaaactt ttaattcatc
     5641 aaatgaattt actttacgca ttatttattc acccctctta acaattcaat tctaattctc
     5701 tataagtatc taatatttcg cttacttggt caggtttcac tttaccataa actttaccat
     5761 ttacagtaac aactggagct aaaccacatg cccccaaaca tcttaaacct tctaaagaaa
     5821 atttaaaatc actagtagtt tctccaactt ttatgccaag ctgtttttca aaagcactta
     5881 agattttgtc agcacctttt acaaaacata cagttcctag acagacactg attttatact
     5941 tacctacagg ttctgtagta aaataagaat agaatgtaac aaccccatat acctttgcag
     6001 gagctactcc aagttttcta gcaacatgaa gttgaacttc ttttggaaga tatccaaata
     6061 taccttgggc ttcatgtaga acttgtatta acgcaccttc ctttgttgca agagagtcaa
     6121 taaatatatc tagttcgtca aataattgct tgttgtgtga aataaaatcg cacataacaa
     6181 atcccccttt tctttttaaa gttattattt ttcataaaat aattaaccat gagttgaccc
     6241 caacattatg tgggtgaaca aaaccaaaac aataaagaaa gaataatata caacaatttc
     6301 tttatttata aaaacaatgc aaaaaagtta cctattaaaa ataaaaacta tactagtcta
     6361 ttataatata aaataattca gaatagttaa aaaaaataaa aaaaatgagc taaattcaat
     6421 aaaaaaaaaa taatacatat aaaaaacttt aaaaaataaa ccaaaatgta aaatttttat
     6481 aaaataaaag atttataaaa ctgctgataa tagtcttgaa attgattcct tgatttttat
     6541 ataagtagac ctagaattat atatatctaa tgttatttcc ttagaattta aaatatcatt
     6601 ttcaaatata attctttgtt ccaatgcttt ttttgaagag tacataaatg cattaacttc
     6661 gaaattaagc tcaaaacttc tgatatccat attagctgtt cctatagagc atatagagtc
     6721 atctataact attgtcttag catgtaaaaa tgcattttga ccataagtat agattttagc
     6781 tccaaacttt aaaagttctc cagcatatga atatgatgcc cagtatacaa agggatggtc
     6841 aggtttagaa ggaatcatca ctctcacatc aacaccagat aaacaagcta tttttaaagt
     6901 atctataaaa gttgagtcaa gtattaaata aggactctgt atgtatatat actttctagc
     6961 tttctgaatc attttaaggt aaccatattt aatctcatca agttctgata tatctggacc
     7021 acttgatact atctgaatcc caatattttc agaaggatga ttagaatttg tatcttcttc
     7081 tataaagtat ttttctaaat ctaagtcttc tttagttgta tatctccaat caaggatgaa
     7141 tctcatattt aggtcaatta cgcaatcacc agtaagctta gtatgtgtat ctctccatag
     7201 accaaatttt ggatttctac ctaaatattc atcaccaaca ttatatccac ctacaaatcc
     7261 taccttacca tcaatgacaa caatttttcg gtgatttcta tagtttaagt taaagtttac
     7321 aattttcaag aatgatggga agaagcttcc aactttaaca ccactttctt taagtctgga
     7381 aagtgtacta ttttttagtg ttctaccacc aactgcatca aataaaagtc ttacttcaac
     7441 accttcttta gctttgtcta taagaatgtc tattatttca gttccgattt tgtcatcttt
     7501 gaaaatgtaa aattgtatgt ttatatattt tttagctttt ttaagttctt caagaagact
     7561 gttaaaaaat tgactagatt cagcataaat ccacacatcg ttattattgg tataatgagc
     7621 attatttgaa ttagccaatg cataaatcat atccttatgt tgatgtatat ctgaatcaat
     7681 ttcagatgta gattggagtt taacctgagt atctagtata ttacttttga ttatcttatc
     7741 atccttttct ttcaatctaa acatattgtt ttttgaaata tttctgccaa aagctatata
     7801 aagtatgaat ccaagagcag gaatcaatac taatataaga agccatgtta aagttgtatt
     7861 tatgtttctt ttttctctaa agataaggtt tagtataacc atgaaattaa ttatatatat
     7921 tgtagtaact gctatttcat atattgacat ttcgaaaaat gatatatcaa acataaaatt
     7981 ccctccttca aatatttatt aaaataagta attat
//
LOCUS       DAELNY010000170         7782 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.173, whole genome shotgun sequence.
ACCESSION   DAELNY010000170 DAELNY010000000
VERSION     DAELNY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.173"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            901..2259
                     /locus_tag="KPE55_003046"
     CDS             901..2259
                     /locus_tag="KPE55_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0494089.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2366..3160
                     /locus_tag="KPE55_003047"
     CDS             2366..3160
                     /locus_tag="KPE55_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0494090.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3153..5459
                     /locus_tag="KPE55_003048"
     CDS             3153..5459
                     /locus_tag="KPE55_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0494091.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5598..6875
                     /locus_tag="KPE55_003049"
     CDS             5598..6875
                     /locus_tag="KPE55_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0494092.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7058..7780
                     /gene="yqeC"
                     /locus_tag="KPE55_003050"
     CDS             7058..7780
                     /gene="yqeC"
                     /locus_tag="KPE55_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG0494093.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
BASE COUNT         3065 a          840 c         1451 g         2426 t
ORIGIN      
        1 acaactgaga tacatagctt attaagtttt tctgtcttat gagaattata ttataaaata
       61 ttcataaata ttttgagttt aaattaaata attattttat gcttagatta attgatattt
      121 aattaattta ataacaaaaa cagtgaaaga gtaaataata ttatttacag ggggtaaagc
      181 aaagtatgtt aaataaattg tacttttggc cccgtacata gagaaatcta tgtaggacac
      241 aactttaaaa ccagtaaatc ctaaagcctt gcaactacaa taaaagatag aaagaaactt
      301 ttatgaatgt ggtgaaaacc agaaacaata gtgaggatgg tatatggtga gagcctaagt
      361 acttaaaaat ggacatttgg tcgccaagcc tagaaatagg aaggtcaaac gactatctcg
      421 agagagagta cactataagc gattgatagt ggaaacgggt tgctcttagc aggtaatgct
      481 gaagatgaag aaatagtcta cgctcatatg aaagtatgag gtcactggat ttgaagtgac
      541 atctataaag tagcgtttat aggtaaatgt ttaaaacttg aaacatgtaa atacttatga
      601 aaacttataa cttataatga ttaagattga aagatttaaa ttgaattaaa tttactataa
      661 aaaattaaaa attcatagct ataatactct tatatttgta aagcatggta tataatgtat
      721 acaaaattaa agaatacgtg tatttgttaa taattaaatt tattgcatct gttttttaag
      781 actaaaaact taaatatatg ttggagtata gttgatattt atgaacatca ttaatttaat
      841 atattagatt tttaagtatt tatataattc aataagtttt atgtaacggg agtttttatt
      901 atgaaagcaa aaagtacatc aaaatttgaa ttagacggaa taccaccact aaaggaagca
      961 atacctttag ggttacaaca cttaatggca atggtaatag gtagttgttt accagcgata
     1021 ttgattgcaa atgcagctgg acttaatcat aagatgtcaa cattattgat tcaagccgca
     1081 ttagtttttg caggattatc aactttatta caattatatc caataccttt gtttaaaggt
     1141 tttaaattag gaggaggaat accagttata atgggagcta cagctatgtt tataggagct
     1201 ggggtatctg ttgcaagtaa gtatggacta ccagtattat ttggctctca aataatagct
     1261 ggtatagtaa taattataat tggttttggt cttaaaaaaa tacgatttat atttactcca
     1321 gctgtatcag gaacaatagt tgcatgtatg ggtcttggac tttttccaat agctataaaa
     1381 aacttagctg ggggtatggg caatgagact tttgggaatc caattaattt tatagttgga
     1441 atatctgtat ctcttatgat attaggatta aataagtatg gaaaaggatt atttaaagat
     1501 gcatcaatat tagttagcat aatttttgga tacattttat cattaatttt gggtatagta
     1561 aatttttctt caattcagga gtttactttg gttgcacttc caaaaccact tgcttttggg
     1621 ctagatataa gacttgaagt tgttgtaatg ttttcaataa tatatttagt tgagatagct
     1681 gatataatgg gtgcttgtac actttctgcg gtaggagggc ttaatagaca agtaacagat
     1741 gaagaacttt cttctgctgt tttaggaaat ggagtactct ctacaatagg tgcattattt
     1801 tctgcaactc caatggggat gtttggacaa aattctgcaa tcgtttccaa tacaaaggtt
     1861 gtaagtaaat ttgtactagc tattggagga attggattat ttttggcagg aatatcgcct
     1921 ttattagcta atttaataag aacaattcct ccctgtgtgg ttggaggagc aacactcgtt
     1981 atattttcaa ctcttacaac atctggttta agacttgtta gtatggatgg atttaatcaa
     2041 gaaaatagca tgatattagg tctttctatg gcttctggta ttggattcat ggtagctcct
     2101 caagttcttg aaaaatttcc aaaatttata gagacattac ttgcagattc aagtgtagtt
     2161 tcaggagcaa tggtagccat aataattcaa gctttatata tggtaaaatt tcctggaaat
     2221 aaaagtgata aggttgagga taaaaataaa agtttataat gtaaaggttt aatatatgtg
     2281 aattaaatat actacaatta taaatataaa taaaagattt attaaaaact tttgtgtttt
     2341 aataagttaa tgatgggggg ataagatggt tactataaag gaatattcag ttccacagag
     2401 tttagaagaa gcatataaaa ttttaatttc taaaaaaaat aatgttattt taggtggatg
     2461 tggatttata aaattaagta ataaaaatat aggaacagcg atagatttaa aagatataaa
     2521 tctaaattat ataaaagagg ataaaaaaaa tattttgata ggtgcagata cttcacttag
     2581 aagtttagaa attgataaaa ctataaaaaa ttactgtagt ggaatacttt caagcgctgt
     2641 ttctaatata gttggtgttc aatttagaca aggggctaga attggtgcaa gtgtattttc
     2701 aaagtatggc ttttctgact taataccagc gcttttggtt gttggagcga aggtgagatt
     2761 atacaataaa ggaatactag agttaagtga gtttttggat agtgaattaa gtagggatat
     2821 attggtcgaa gtaatactac caaaaaaaga tgcaatagct gtttttgact caataagaaa
     2881 atgcacagga gatttttctg tattaaattc agcaatgcta aaagaaaatg atacttacaa
     2941 aatagcaatt ggggcaagac ctcaaaaagc tagattagca cttgaagcga gtaatatatt
     3001 aaataaagaa aaagatatag ataaggcatc tattgttgct agtaaggaac ttacttatgg
     3061 aagtaatatg agagctagta aagagtacag aaaggatatg gcaagtgcat tagttaaaag
     3121 gatgtataat gctattgagg agggaatgta taatgattaa taaggggata agaaagatag
     3181 atagtacagc aattgtcaca ggaaaacctc tatatactga agacctcatt atgcataaag
     3241 atgttttgac attaaaacta cttagaagtc cacatgctca tgctaagata aagaatatag
     3301 atacatcaaa agccgaaaaa attccagggg ttgtgggtat ttatacgtat aaagatgtac
     3361 cacaggtcag atattgtgtg ccaggtcaaa cagaaccaga agcatcacct tatgatagag
     3421 ttttacttga agatacagtt agatttattg gagatgaagt agctttagtg gctgctgaag
     3481 atgagaaaac ggcacttaaa gccttaaaaa tgataaaggt agattatgag gttttaaagc
     3541 ctgtgtttga tgcaagagaa gcagaaggaa atgaagttgt tatacatcca gaaaaagaca
     3601 tagtagagcc atatcctttt ggattggatg caaaaaataa tatagtatca aaagaatttt
     3661 ttgaatttgg agatgttgaa gctaagctta aagaaagtga ttttgtggtt gaatcttcat
     3721 acaaaaccca aagtcaatcg cattgcatga tggaaactca taggtcatat tcatataaag
     3781 atataaatga tagaattgtt gtagtttcat cattacaatc accacacaat gtaaagagaa
     3841 tacttagaaa agtattgggt ataccatctt caaaaataag agtaattaaa cctagaatag
     3901 gtggtggatt tggaggaaag aatatagcag caacagatat atttactgca tttgttacta
     3961 tgaaaactgg tagaccagca aaatttgtat ttacaagaag tgaaactttt gtaggttcta
     4021 atagtagaca tgaaatgttt tttgatgtta aagttggtgc agataaagaa ggaaatttaa
     4081 aagcaataga gctaaagtca ttaaacaata caggtgctta tggtgggaat ggtgtctctg
     4141 tttcttccga atctggacac aataccttac caatatataa tgatattgat gctgttagat
     4201 ttaatgctag aacagtatat actaataggt taccagcagg agcacttaga gggtatggtg
     4261 ctacacaggg aatatttgca atagatagtg ctattgatga attggcacat aagattggaa
     4321 tggatccact tgaatttaga attaaaaata taatagatag gtataccaat ggaaaagcag
     4381 ctgaagacga aatattaagt tgtgagctga gacaatgtat agaaaagggc aagaaattaa
     4441 ttaattggga tgataaatat ccatgtaaag atataggaaa caacaaagta agagcagtag
     4501 gtatggcagt agcaaatcat ggttctggta taccgcgtgt agatatggca acagtcacta
     4561 taagaatgga tgaagatggc acatataaac tattatctgg tcttgctgac ttaggtcaag
     4621 gggctgatac aattcaagct caaattgctg cggaagcttt aaatactact gtagatagaa
     4681 tttctattta tacaggtgat tcagacttat gtccctatga tacaggagca tttgcttctt
     4741 gtacgacaac ggttacagga aatgcaacaa taaagacagc aaataaatta aaagatatat
     4801 tactttcagt tgcagagtca aagctaggat ttaaaaaaga agatttagag ttgggaaaag
     4861 acagggtttt tgttaaaaac gataatagca agtttgttct attagaggaa ttgggtagag
     4921 aatctgttgg aggaatagga tttgctagtg atgatgtacc agtagttttg gaagcaacag
     4981 aatcttttgg catgttgaaa aaaataaaac catttatagc aggatttgca gagatagaat
     5041 tagataaaaa tacaggcgaa tataaagtca taaattttgc atgtgtacct gattgtggaa
     5101 cagttataaa tccaacttta gctagaatac aagtagaagg tggtgtgatg atgggtatag
     5161 ggtttgcaat gtatgaagac cctatatttg ataaagatgg tagattatta acagatagtt
     5221 tcctacagta taaagttcct acaaaaaaag aagttggaaa tatattggta gattttgctg
     5281 agaatttcga cccaggaggt ccttatggtg ctaaatcatt aggagaaata gttatacata
     5341 caccagcacc agctatagca aatgcaatat ttaatgctac aggagttcgt ttaagagagc
     5401 ttcctatgac ttttgaaaaa gtttatatgg gtatgaaaaa taataataaa caattataat
     5461 aaataataag atgtataaaa gcaagtaaat ttaaattatt ttgtaaaata aagtaattaa
     5521 atggacatta tatatggtgt tttgtaaaaa taccgtatat aatgtctagt agagtatatt
     5581 ctaaaggagg tgagttcatg tcaaacagaa taaaaaatat acactatgga actcagagac
     5641 aacaattaaa aaacttaact aaaaaagaat atcaagctct acgagaaatg tgcttcttag
     5701 ctaagaactt atataatgtt ggtacatata atgtaagaca gcattatttc aatcaaggag
     5761 aacatcttaa ctatgaagct aattatcatg tatgcaaaga aaatgagaac tataagcttc
     5821 tcaatagtaa tatagcgcaa caaatattaa aagaagtaga cagttcgttt aggtcatttt
     5881 ttggattaat taaattagcc aaagaaggaa aatatgactt tagagggata aaatcaccta
     5941 aatatttaga taaagaagga ttttttagca taataatagg tcaaataaga atcaaagaag
     6001 atgggatttt aaatgtacct atgtctccag catttaaaaa actatatggg aaagtaagta
     6061 taaaagttcc acaaaatata ctagagaaaa aaattaaaga aataagaata atacctaaac
     6121 ataaagctag gttctttgaa attcagtatt gttatgaaat accaaaatca gaggaagttt
     6181 caaatcaaaa aaatgcacta gctatagact tgggtttaga aaatttatgt acatgtgtta
     6241 ccaatttagg tgatagtttt atcatagatg gaaaaaggct aaaatctgtg aatcagtggg
     6301 caaacaaaca gaatgataaa atagaagcct taaaattaaa aaagaatata actgcaataa
     6361 caagaaaaca atttaaagtt tggaataaga gaaataatca agttaaagac tatgtaaata
     6421 aaacctgttt ttatataatt gattattgtg taaaaaataa tattggaaat ttaattgtag
     6481 gatataatgc taagttagaa aaaagtgata atatggagag aaaaatatat agaaatttta
     6541 taagaatccc atttggagag ataaaaggaa aattagaata tctgtgtaaa ataaaaaata
     6601 tcaattttat taggcaagaa gaaagctata ctagtaaatc agattttttt gctgatgatt
     6661 ttctacctga tatcaatgtg gacaatgtta aaaaatatga ctttaaaggg gaaagaataa
     6721 cgcgtggaca gtacaaatcc agtgttggaa tcattataaa tgcagatgta aatgcgagtc
     6781 ttaatatact aaaaaaatca caagtagtta atttatctaa tttacagaat aatcctcaaa
     6841 tattaaagca accacagaga gtaagaatat cttaattata tttaaaagat aaactgatag
     6901 cgtaagctta cagcttagcg gcgaattgga tccgccttgt tgtttacaac agaatctatt
     6961 tactttcgag tttatggaat ttcaaaaatc acttttacta aaaaggtcta actataataa
     7021 ttaagagatt agaaaactgg ttataagagg tgaaagtgtg gaactatcaa atataataga
     7081 aaagaatgaa ataataactg tagttggagc aggaggaaaa acttcgctta taaactattt
     7141 tgcaaatttt tatagagata aattgaaagt tctccttaca acaacaacta aaatatatgt
     7201 accaaacgat tatgacaata taattattac aatagatgga actgttattc catctatttg
     7261 tcatggaata acagtctgtg gcagttatat aaataatgaa aataaattag taagtattga
     7321 ttcctctatt ttggatgaaa ttgtagacca atttgattta gttttaatag agggagatgg
     7381 ctcaaagaga aaaaaactta aaggttggaa tgctaaagaa ccagtagtat atcataaaac
     7441 tacaaaaacc atagggatat tagatataac atcatttgga atgaatatta atgaagagaa
     7501 tatacataga gtagaaatat ttaaaaaaat agcaaattta gatacaagta gtattaatag
     7561 tagttctaca gtaagtattg aaaacttaaa aaatatagta ttaaatccaa atggattatt
     7621 taaaaattat tctggaaaaa gagtattatt tattaataaa gttgaaaatg aaaaatataa
     7681 aaatttagct ataaaattaa tagaaaatat taaagaatat gaaagtgaaa tagagatatt
     7741 ttatggaagt gtaaaacaaa aattttgtgt aagatattga ag
//
LOCUS       DAELNY010000171         7718 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.174, whole genome shotgun sequence.
ACCESSION   DAELNY010000171 DAELNY010000000
VERSION     DAELNY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.174"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1452
                     /locus_tag="KPE55_003051"
     CDS             <1..1452
                     /locus_tag="KPE55_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0494094.1"
                     /translation="EVDIKVLILILLTLFSINVFISYLKIKLYEDKINLGLINNSDKF
                     INITKDENFLKHVQNYIISNEKENCVMACFDLCRLKLINDIYGYELGDEVLNNILSNL
                     KNYFGKEAIYGKLKSDVFVLIIKLENREQKIPYLVNLIKNKIVILSQIDSFKMDINIE
                     ASIGIYKFNNNEIDIKKAIDNADMARLKSKGLKHIEYVEFDNAMEEEIQSIMKIERDL
                     FLAIKNKEFVIHYQPKVDSSTGNIIGSEALIRWLHPSLGMVGPNKFIPIAEKNGLINN
                     IGRWLIQEVFITINKWISEGINVLPVSINLSRVELYKNDLVDFFKLMFNTYNIPKELI
                     ELEITETTALRDVKFISERLYEIKSLGMNISLDDFGVGNSNFINLKGIPLDIIKIDRS
                     LILDIVTNTKTKFMVKAIVNLSHDLNVTVICEGVEDMHQVKVLSELGCNFIQGYVFFK
                     PLDEMNYKKLLTDGSYINLKDTLLDKCMTVEEE"
     gene            1455..1853
                     /locus_tag="KPE55_003052"
     CDS             1455..1853
                     /locus_tag="KPE55_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0494095.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1893..3626
                     /locus_tag="KPE55_003053"
     CDS             1893..3626
                     /locus_tag="KPE55_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0494096.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            3858..5069
                     /locus_tag="KPE55_003054"
     CDS             3858..5069
                     /locus_tag="KPE55_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0494097.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5262..5522
                     /locus_tag="KPE55_003055"
     CDS             5262..5522
                     /locus_tag="KPE55_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG0494098.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            5876..7588
                     /gene="ptsP"
                     /locus_tag="KPE55_003056"
     CDS             5876..7588
                     /gene="ptsP"
                     /locus_tag="KPE55_003056"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG0494099.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVFNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         3202 a          682 c         1345 g         2489 t
ORIGIN      
        1 gaggtagata ttaaagtttt aattttaatt ttattaacct tgtttagtat aaatgtattt
       61 atttcatatc ttaaaattaa gttgtatgag gataaaatta atttaggatt gataaataat
      121 tcagataagt ttataaatat tactaaagat gagaacttcc taaaacatgt tcaaaattat
      181 attatcagta atgaaaaaga aaactgtgta atggcttgtt ttgatttatg tagactcaaa
      241 cttataaatg atatatatgg ttatgagcta ggggatgaag tgttaaataa tattttaagt
      301 aacttaaaaa attattttgg aaaagaagct atttatggaa agttaaaaag tgatgttttt
      361 gtattaataa taaagcttga aaatagggaa cagaagattc cttatttagt aaatcttatt
      421 aagaataaaa ttgtgatact aagtcagatt gacagtttta aaatggatat aaatattgag
      481 gcttctattg gaatttataa atttaacaat aatgaaattg atataaaaaa agctatagat
      541 aatgctgata tggcaagact taaatcaaaa ggactaaagc acatagagta tgtagaattt
      601 gataatgcta tggaagaaga aatacaatca attatgaaaa tagagagaga tttattcttg
      661 gctattaaaa ataaagagtt tgtaattcac tatcaaccta aagttgattc aagtacaggt
      721 aacattatag gttcagaggc attgataaga tggttgcacc catcattagg catggttgga
      781 ccaaataagt ttatacctat agctgaaaaa aatggtctga taaacaatat tggtagatgg
      841 ctaattcaag aagtttttat taccattaac aagtggattt cagaaggtat aaatgtctta
      901 ccagtctcaa taaatctttc tagagtagaa ctatataaaa atgatttagt tgattttttt
      961 aaactgatgt ttaatacata taatattcca aaagaactta tagaattaga aattacagaa
     1021 acgacagcac taagagatgt aaaatttata agtgaaaggc tttatgagat taaatcttta
     1081 ggtatgaata tatcattaga tgattttgga gtaggtaatt caaattttat caaccttaag
     1141 gggataccac tagatattat aaaaattgat aggtcactta tattggatat agttacaaat
     1201 acaaaaacaa agtttatggt gaaagcaatt gttaaccttt cacatgattt aaacgtaact
     1261 gttatatgtg aaggggtaga ggatatgcat caggtcaaag tcttaagtga actgggatgt
     1321 aatttcatac aaggttatgt attttttaaa cctttggatg agatgaatta taaaaaactt
     1381 cttactgacg gaagttatat aaatctgaaa gatactcttt tagataaatg catgacagtt
     1441 gaggaggagt aaggatgatt tcagaagatg tagattattc aataagaatc ataagagcta
     1501 tatttaataa tggagaagat aaaccaatag atgctaaaat gatattaaaa agtgaagata
     1561 taccaatact aacactatac agactgctta gaaagctttg tgcaaacaat attcttactt
     1621 ttaacaaaga agatggcaag acatacatgt taaatgtgtc attggataca ataagtctaa
     1681 gaactattgt agaagtaatt gatggaaaaa tatatataaa taaatgtacg agatatgtat
     1741 ctaattgcag taattgctta aaaggttgta aagtaaatcg tgaattgcta gaaatagaaa
     1801 aagaaattta taaactttta tcgagaaaat ctataagaca aatactagaa taaaaaattt
     1861 tcagctatga taataattgg aggttttcgg ttatggatga tatatctcaa gacaattttc
     1921 tactgtctaa agagtatgag aatagcttag atgtagatac taaaaaagca agtggaatat
     1981 attatactcc taaaataatt gtagattata ttgtaaaaaa aactttaaaa aatcacgata
     2041 taattaaaaa tccatatcca cgaatacttg atatttcctg tgggtgtgga aattttcttt
     2101 tagaagtata tgatatttta tatgacttat ttgaggaaaa tatatatgaa ttgaaaaaga
     2161 agtatgatga aaattattgg actgttgata acatacaccg tcatatatta aactattgta
     2221 tatatggtgc tgatattgat gaaaaagcta taagtatatt aaaagatagc ttaacaaata
     2281 aaaaggtagt aaatgattta gatgaatctg atataaagat taatctattt tgttgtgata
     2341 gtcttaaaaa gaaatggcga tataagtttg attacatagt gggtaatcca ccctatattg
     2401 gtcataaaaa acttgaaaag aaatataaaa aatttctcct agaaaaatat agtgaagttt
     2461 ataaggataa agctgactta tatttttgtt tttataaaaa gattatagat attttaaagc
     2521 aaggagggat tggcagtgtt ataactccaa gatatttttt agaaagtcta tcaggtaaag
     2581 atttaagaga atatattaaa tcaaatgtca atgttcaaga aatagtggat tttttaggtg
     2641 caaatatatt taaaaatata ggggtatcta gttgtattct aacttttgat aagaaaaaaa
     2701 caaaagagac atatatagat gtatttaaaa tcaaaaatga ggatatatgt ataaataaat
     2761 ttgaaacatt ggaagaactt ctaaagagta gcaaatttga acattttaat ataaatcaga
     2821 gattgctatc tgacgaatgg atacttgtaa ataaagatga tgaaactttt tataataaga
     2881 tacaagaaaa atgtaaatat agcttggaag atatagcaat aagttttcaa ggaataatta
     2941 cagggtgtga taaagcgttt attttaagta aagatgatgt aaagttgaac cttgtagatg
     3001 ataaattttt aaaatgttgg ataaaaagta agaatataaa taaatatata gtagataaaa
     3061 gtgaatatag gctaatatac tcaaatgata tagataatga gaatacaaat aaaaggatac
     3121 tagatgaaat tatagggcta tataaaacaa aattagaaaa cagaagagaa tgtaaaagtg
     3181 gaataaggaa atggtatgaa cttcaatggg gtagagaaaa gcttttcttt gaaagaaaaa
     3241 agataatgta tccttataaa tcaaatgaaa atagatttgc tatagattat gataataatt
     3301 tttcaagtgc agatgtatat tcttttttta taaaagaaga gtatttagat aaattttcat
     3361 atgaatatct agttggaatt ttaaattcca gtgtttatga taaatacttt aaaataactg
     3421 ctaaaaaaat gagtaaaaat atatatgatt actatcctaa taaggttatg aagataagga
     3481 tttttagaga taataattat gaagagatag agaatttatc aaagcaaata atatcaattt
     3541 tattaaataa gtctatagat aaaggaaaag tagaaaaatt gcagataaaa atggataact
     3601 tgataatgga ttctttaggt atataagcag ttgtaagtat taaaaaatta taatctttta
     3661 attgtttatt taaatttaga aataaaaagt tagccatata aaaaatatta aaaatatata
     3721 cttttcaata tttttttgat aaaataagaa tgaatttgtg caattttatt tttctccaat
     3781 aactaaaaat agttttttga attaatattt gcttaagaat aaaaatcaga aacgtttttg
     3841 atatagtagg tgataatatg aaaggattag ttttagaagg tggaggaaca aaaggagcct
     3901 accaaatagg tgcatataaa gctttaagag agcttggtat agagtttcaa ggagtaactg
     3961 gcacatcaat tggtgcttta aatggagcat atatagttca aaatgatatt gaagtaatgg
     4021 aagatatttg gttaaattat gactatactc attttatgaa tattgataga gaaacctatg
     4081 agaggtttaa aaatgtagat ttcactacta aaaatctcaa tgcagtgttt gagcttataa
     4141 ataaagctag aaaaaatgaa ggtatagata taagcccatt aagacaacta atggagacaa
     4201 cattaaatga agacataata aggaaatcaa aaaaagactt tggacttgtt acggtctggt
     4261 gggataaaaa aattaatcca tatccattgt atatcgaaga tatacctaaa ggaagattga
     4321 ttgattattt aattgctagt tccagtttgc ctatttttca aacgaacatg atggatgata
     4381 agttgtatct tgatggaatg ttttgtgata acatgccaat aggactcttg gaagacaaag
     4441 gttatgaaga tgttgttgta attaggttga ttgatgattt tattgggaaa atgaatctaa
     4501 atcgacatca aaatataaat ataaaaacta taataccatc agaatatcta ggaggttctc
     4561 ttaataaaga tagagataat gtagaaaaaa acatccgact aggttactta gatactatga
     4621 aagcatatga aagatgcact ggaattaagt attattttga tgtagagtat aaatataatg
     4681 aagaatattg ttttgataaa tttagaagta tgagtaatga aacaatacaa tatatctgta
     4741 atttattgaa tataaaaaga gatgcttcac ttagaaccgt tatggaaagt gtaataccaa
     4801 agcttggtga ggtgcttgga ttgccaaagg atttttcata taaagatttg ttttactgta
     4861 tatatgaaaa gaaacttgaa gaaaataata taaacaggat aaaattatat gattttaaca
     4921 aggttataga tgttgtaaat acaaatattg attctaatag aaatttagct tctaattttg
     4981 aagttagacc taaaattagt attaaaaaga acaaaaacac aaaattattg acaaattgta
     5041 ttataaaaga cataaaaaat caaagttaaa taaaaaacaa aaaaaaattt gtaattatac
     5101 tcgaaaaaag tgtatattag tagtataata tttttgtagc actagtggga acaaaaaatg
     5161 accataggac aaaaaagtac cacttagtac cattaaagta aaaaacagga aaacaatttt
     5221 aaaaaatata aaatgtaaag tttataggag gatttaaaaa aatggaaaaa gtagttagta
     5281 tcaaaaatgc aagtggatta catgctagac cagcaggaat gtttgttaag aaggcagctg
     5341 aatttaaatc aacagtagag gttgtagcaa aagacaaaac tgtaaatgca aaatcaataa
     5401 tgggaataat gagtttaggt ttagctcaag gagaagaatt aaagttagtt gtaaatggag
     5461 aagatgaaga agtagcttta gctgcaatgg ttgaattagt tgaaagtgga tttggagaat
     5521 agatataata attttttaaa aatttgatat ttagaatcta atattactta aagtaaagta
     5581 acagagaagt attaaacata tacagaccaa catttgtaga tatttaattt ctttgttact
     5641 ttcatgttta tttaatcaga agattaagac tattgttaat tgggtataaa aatataaata
     5701 ttttagaaaa tgaaataaga tatttttaat cataaattca gagtaaaaat acagtaacaa
     5761 atagtttata aaattttata aaaaattata agtaaataaa actataaaaa tgtaaattat
     5821 ttatataaat ttataaagtt tatgtgctag atgatatttt ttaggaggta gggttatggc
     5881 ttacaaagga ataggtgctt ctcctggtgt tgctttaggg aaagcgttag tagttgaaca
     5941 tagtgaatta gtaattgaaa aaaaatctat agataatgtt gaagctgaaa ttgctaaatt
     6001 agaagatgca gttgcagttt ctaaagaaga attagtaaaa gttaaggaaa aggcttctga
     6061 agaattagga gcagaggaag cagaaatatt tgaagcacat ttattagttt tagaagaccc
     6121 agagttaatt ggttctgcaa tagataaaat taaaactgaa agtgtaaatg cagagtatgc
     6181 tttaaatgaa ataaaagaga tgtttgttag catgtttgag tctatggaca atgagtacat
     6241 gaaagaaaga gcggcagata taaaagatgt tacaaataga atacttagac atatattagg
     6301 aattaaagtt gttgatttat ctgctttaag cgaagaagtt gtattaatag cacatgattt
     6361 aactccatca gacacagcaa ctatgaataa aaaaatggtt ttaggatttt taactgatat
     6421 aggtggaaga acttctcata ctgctatcat gtctagaact ttagaaatag ctgcaatcgt
     6481 tggattaaat gatataactt caaaagttaa ggatggagat tttgtggttt tcaatggaga
     6541 cacaggtgaa gtaatcgtga atcctgatga agagactata aataaatata ctgaattaaa
     6601 agctaagtat gaagatgaga gaaaagcatt acagctatta aaaggaaaac catctgtaac
     6661 tttagatgga aaacatgtgg aattagctgg taacatagga acacctaacg atatagaagg
     6721 tctaataaag aatgatgctg aaggtgttgg tctttataga acagaatttt tatacatgga
     6781 tagagattct ttcccaacag aagaaataca atatgaagca tacaaagctg tattagaagg
     6841 aatggatgga aagcctatag ttataagaac attggatata ggtggagata aagaattaag
     6901 ttatttaagt atggaacctg aaatgaatcc attcttaggt tacagagcaa taagactttg
     6961 cctagataga aaagatatat ttaagactca attgagagct ttatatagag ctagtgttca
     7021 tggaagatta agaatcatgt tcccaatgat ttcatcttta gaagagttgt tacaagctaa
     7081 agaagttgta aaagaagttt tagcagaact tgattctgaa ggtgtggctt atgctaaaga
     7141 tgtagaaatt ggtatgatga tagaagttcc atctgctgct gtgatatctg atgtattggc
     7201 taaacacgta gatttcttct ctataggaac taatgattta attcaataca cttgtgcagt
     7261 tgatagaatg aatcaaaaga taagttatct ttacaatcaa tttaatccag ctgttttaag
     7321 acttataaag actgttatag acaatgcaca taaagaagga aaatgggcag gtatgtgtgg
     7381 agaatctgct ggagaccaaa aaatgatacc aatactttta ggtatgggat tagacgagtt
     7441 ctctatgtct ccaatatcaa tacttcctgc tagaaagtta ataacttcag ttaaagagtc
     7501 agatatgaaa aaattagctg atgatgtatt taatatgggt actgctgaag aaataaaaag
     7561 ctatatagaa aagactttta atatataatt aatgttatat agttactaaa attcattttt
     7621 ctaaataaca aaattatatt ttacgaataa aaaaactata gtatatcata tatactatag
     7681 tttttttgtt atataaatta cgtatcaaaa tttgtaaa
//
LOCUS       DAELNY010000172         7710 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.175, whole genome shotgun sequence.
ACCESSION   DAELNY010000172 DAELNY010000000
VERSION     DAELNY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.175"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..336)
                     /locus_tag="KPE55_003057"
     CDS             complement(<1..336)
                     /locus_tag="KPE55_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0494100.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDN"
     gene            1433..2347
                     /gene="queG"
                     /locus_tag="KPE55_003058"
     CDS             1433..2347
                     /gene="queG"
                     /locus_tag="KPE55_003058"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG0494101.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            2453..4690
                     /locus_tag="KPE55_003059"
     CDS             2453..4690
                     /locus_tag="KPE55_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0494102.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            4887..5939
                     /gene="nth"
                     /locus_tag="KPE55_003060"
     CDS             4887..5939
                     /gene="nth"
                     /locus_tag="KPE55_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG0494103.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMETIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            6133..6603
                     /gene="trmL"
                     /locus_tag="KPE55_003061"
     CDS             6133..6603
                     /gene="trmL"
                     /locus_tag="KPE55_003061"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG0494104.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            6639..7487
                     /locus_tag="KPE55_003062"
     CDS             6639..7487
                     /locus_tag="KPE55_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0494105.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         3263 a          675 c         1416 g         2356 t
ORIGIN      
        1 gttatcttta tctataaaat caaataaaag tactggctta agttgtttat cattttttat
       61 tattccactt ttttcaatac catcacaaat actttttgga taattatgta aatcccccat
      121 tctcttattt gggaagaata atttcaagac tgttattaga ttttcttcaa cagtttcccc
      181 ttgatactgt tggtttataa aacctgcaat catgttttcg tatgcaagat acttagaata
      241 ttttcccttt gaaggcatcc atgcttgacc atttatgtta tgaagcttaa aattagattt
      301 actaataggt ctacctggaa ttgttatttt aatcaaattt ttcaccatcc ttatacagta
      361 tttattataa tctgtatatg atttaaaagc aaattgttat ttaaagtgtt ttacagagct
      421 tttttgtaaa attttccact ttagatgtag taatataaga taagtatttt gcttacaaat
      481 tttaaaattt gatatatgat ggacatattc atattagact attataagac ctttataata
      541 ttaataagta tagtttagta aagagagtaa aaagaaatcc atataagtca agtaaagact
      601 taataaatgg caatgtaaat gggatattaa ttataagaaa aaatcaatct ttttgcttaa
      661 ctatagaaaa aatgtcaaaa tataactaat gatatttgaa gttttagagt ttatattaaa
      721 aggtaagaag gttaagtggg taaaaaaggt tgaatagatt aaaaaggcta agtatctaaa
      781 agagttaagt aggtaaaaaa gttaaataag taaatgaaaa aggctaagta tctaaaagag
      841 ttagtaggta aaaaagttaa ataagtaaat gaaaaaggct aagtatataa aggagttagt
      901 agataaaaaa attaagtatc taaaagcgtt agtagataaa aaaattaaat aaataaaaaa
      961 gactaagtat tcaaaagagt tagtaggtaa aataattaaa tagataaaaa gactaattat
     1021 ttaaaggagt taagtagata aaaaagttaa ataagtaaat gaaaaagacg aagtatctaa
     1081 aagagttagt aaataaaaaa gatgaagtat ctaaaagagt tagtaaataa aaaagatgaa
     1141 gtatctaaaa gagttagtag gtaaaaaact aaataggtaa aaagatgaag tatctaaaag
     1201 agttagtagg taaaaaatta aataggtaaa aagacgaagt acctaaaaga gttagtaggt
     1261 aaaaaaatta aatagataaa aagacgaagt atctaaaaga gttaagcagg taagaaaatt
     1321 gaatagctaa aaagattaag tatttaaaag agttagtagc tagaaaaggt taaaaagact
     1381 aaaaaaatta aataaattta aaaagtcaat tagattaaaa aggaataatt ttatggataa
     1441 taaggaatta aaagcatttt gtaatttaat aggattaaac tgtgtaggag tagcaggggt
     1501 agataagtat gataatttag aaaagatact taaggacaga caacaaaaag gttatttaac
     1561 tggtatggaa gaaccaataa ttgagaacag aattaaccca agacatataa tgaaggatgc
     1621 taactctata atagtctgtg catttccata tcatataaat agacaagaaa agaataaaaa
     1681 ttcaaattta tcaaaatatt gtcatggaaa ggattatcac attgtagtaa aggacttttt
     1741 acaacaaatt tgtgattata tttctaaaac agtaacagat tttaaataca agttatttgc
     1801 tgataatgga ccattagttg acaggtattt agcttattta tcaggaatag gatattttgg
     1861 aataaataat aatatcataa ctgatgaata tggttcttac gtatttatag ggtatatagt
     1921 aaataattat aaatttgaag aagataaacc actagaaaaa agttgtttta aatgtggtaa
     1981 gtgtgtaaaa tattgtccgg gaaatgctat attgggaaat tatgatatga accccaaaaa
     2041 atgtttatca tatataactc aaaaaaaagg agatttgtca gagaaagaaa aagttgtttt
     2101 aaagaatggc aaaaaggtat ttggatgcga tatttgtcaa gaagtatgtc cacataatgt
     2161 tgaaatacca acaactcata ttttggaatt taaagaaaat ataatagact atttggatta
     2221 tgatgagata caaaatattt caaataaaga atttaaaagg agatatggag atagagcttt
     2281 tagctggaga gggagaaata taataaagag aaatatggaa ataattttag ataagtatga
     2341 tgactaataa taagagtctg tttcatatac tttaatgtgt atttacttta acacttgttg
     2401 tgtaaaagtc atattatatc attaaagtta aatatgggga ggtgggggaa tattgaaata
     2461 taataataca tctgaaataa atccactaga ttatatattg ggtcaagaaa gagctgtaga
     2521 agctatggaa ttagggttga aaataaataa tccagcgtac aacatatata tagctggtga
     2581 acctggaact ggtaaaagta cttatgcctt aaaggtttta aatgagtatg catccaaaaa
     2641 aaatacgcat aaagattggt gttatatata taattttgaa aaccctagag agcctattat
     2701 agttgaatta gaaaaggggt ttggaagaga acttaaaaaa gatatggaga aattaattga
     2761 aagtctttta gaagatttta aaaatgcgtt tgaaagtgaa aattttgaaa tagaaaaaaa
     2821 taaacttctg gatgaatatg aaatagaaaa agatatgctt ttgaagcaaa ttaagaaata
     2881 tggagaagaa aaaggattta aattaaaaca gagtaaaatg ggaatagttt ttatcccttt
     2941 gaaagaagag gaagatgaaa gtgatgagtc tgatgaagag ttttacaaag ctaaaagaga
     3001 actagaaaat atggcgatac aggtcgtata taaaattaga aacttagaag aaatagctga
     3061 aaaagcagtg ttagagcttg aagaagagat agctaaactt gtagtagaac ctcatatcaa
     3121 gagattgtgt gaaaaatatg aaaattatga taagattcaa acttatctgg aaaatataaa
     3181 gaaagatata atagaatata tgtatttatt ctatttagat gaagaggagt taaaagataa
     3241 gtatgataaa gaacatttta ttaaatataa aattaaccta tttgtagaca atggaagctc
     3301 aaagaataaa gaatcagcac ctgttgtagt agagataaac ccaagtccag caaatctatt
     3361 tggaaaggca gagtatgatt atgcaaatgg aaatataaag actgatttca caaaactatt
     3421 atcaggagca tttcataggg ctaatggtgg ttatttagta ctttatgcag accaattact
     3481 aaaatataca atgtcttggg aaattcttaa aaagactttg cagactaaaa aagtaatatt
     3541 ggaaacacaa acagcaatta aaccagaaaa tatgccattg gatgttaaat tagtactgat
     3601 tggaagccat tatatatatg atattttata tagatatgat gaagattttg aaaaatattt
     3661 taaagtattt gtagactttg atagtgaaat ggataaaaat gaagataatg aagaaggaat
     3721 agcaagattt atagcatacc aatgtgataa gaataattta aggcatttta ctaaatcagc
     3781 agttgaagag gttattaaat ttagcactag attatctggt gatttagaaa aactatcaac
     3841 taagtttaat aaaataatgg aagttgtaat agaaggaagt gcttatgcag agtttagaaa
     3901 ttcggagtat acagagcagt gtgatgttaa aaaagcaata agtgaaaaga gaaaaagaat
     3961 caatagagtg gaaacacata tggatgaatc aattgaaaat ggatttactc taatagaaac
     4021 agagggaaga aaagtaggcg tgataaatgg tctgtcagtc ttaagtacag gagaatactc
     4081 ttttggtaga gcttcaagaa ttacagcgac cactagccca ggaagtaaag gcatagtaaa
     4141 tatagaaaga gaagtaaata tgagtggctc tatacataac aaaggagtac ttatattagg
     4201 tggctatctt tctgagaatt ttgcacagga tttactattg tcactaaatg catatgtttg
     4261 ttttgaacaa aattatggtg gaatagatgg agatagtgca tcttctgcag aattatatgt
     4321 actattatca tctcttagtg gagtatcaat taaacaaaat attgctgtta caggttctat
     4381 aaatcaaaag ggtgatatac aagtagttgg tggtataagt gaaaagatag aaggatttta
     4441 ttctatatgt aaaactaaag ggttaaatgg tgagcaaggt gtcataattc ctagaaaaaa
     4501 tcaaagaaat ctcgttttat cagatgaagt taatgatgca gtgcgagatg gaaaatttaa
     4561 gatatatact gttgaaagag tagaagaggc tatagagata ttgacagatg ttaaatttga
     4621 agaaataaag aaattggtaa aagaaaaatt aatatctttc agcaagatac aaactgtgtc
     4681 aaaagaataa gtttattata gaatttaatg ggtatttatg tataataaaa agcaagaact
     4741 ttaataggtt tttgcttttt ttgtaaatat atataaatta aattcttatt ttgtatgttt
     4801 tatatattga tgtttatttt ttgttgatat gattaaacta tttataaata gcgttttata
     4861 aattagtatt ttaaaggggg ataattatga aaaatgtaaa tctaaaagag ataaatgaaa
     4921 aagatgtaat tgtggaagaa gatataaatc taaaaggaat ggataagaaa gatgtaattg
     4981 agaaggaaga agtaaataaa aaaagtatag ataagaaaga tataattgag aaagaagaga
     5041 tagataaaaa aaatgtaaag aaggtcgagg ttcaaaaagg aatagataaa atgattggta
     5101 aaaaagatga gataattgat gagaaagata tagagaattt tatggatatt gaagaagata
     5161 aaaaaagtat caaagtaagt aaaaaaagta ctaaaaaagg tgaaacggag aaaaaaacaa
     5221 agaagagtag ttctaataag gctacacaaa aaacaagtaa agaaactaaa agtaagtcta
     5281 aaattaaaaa tgagtcagca aatgaaaaag acgtaaataa gattttagac gagttagaaa
     5341 aattgtatcc agatgctaaa tgtgagttaa attatgggac agcctttgaa cttttaatag
     5401 caactatttt atcagcacaa tgtactgatg taagagtgaa taaagttact tcagagttgt
     5461 ttaaaaagta caacacagct agagactttg ctaatttgag cattgaagaa attagtaaag
     5521 agataaagag ttgtggtctt tacaaaagca agtctcaaaa aataaaggat acatctgaac
     5581 agctatgtga attatatgat ggtgaagtac ctgatagctt agaaaagctt ataaaattac
     5641 ctggtgttgg aagaaaaacg gcaggggttg tacttagtaa cgcttttaat catccagcaa
     5701 tagctgtaga tactcatgta tttagagtta gcaatagaat aggaatagtg gacgagccaa
     5761 atcctcaaaa aacagaattt gcacttatgg aaactatacc aaaagaaaga tggtcacact
     5821 cacatcatgt attaattttt catggaagaa gaatgtgtaa agctagaaat ccagaatgtg
     5881 caagttgccc aataaaagaa gattgtaatt attataaaga acttaatgaa acgaaataac
     5941 ttttagaaag taagttgtac aagagggatt ttctgatata ataatacttg tgaaaatgtt
     6001 agataaaaaa ctattatata catgaaataa aaattattat taacattatt ttatataata
     6061 gtataatatt attgttattc tgtttataaa ataaaatttg ataaataaaa gcaatactta
     6121 gggaggatta aaatgtcatt aaatatagta ttagtagaac cagaaatacc tcaaaataca
     6181 ggaaatataa taagaacctg tgcagcaaca ggttcaactt tacatttagt aaaaccactg
     6241 ggtttttcat tggatgataa acacctaaaa agatgtgggc ttgactattg ggatatagct
     6301 gatatacaat attacgatag ctttgaagaa ttacaagaaa agtatccaaa tagtaaatac
     6361 tttttttcta caacaaaggc taaacagtca cattcagatg tgaaatatga agaaaattgt
     6421 tttatagtat ttgggaaaga aacaaaggga ttaccagaac ctttgttgaa agcaaatatg
     6481 gatacttgta ttagagtgcc tatgttggat gttgaaaaag ctaggtcatt aaatttatca
     6541 aattcagttg ctattgtagt gtacgaggct ttaagacaaa taggataccc aaatatgagg
     6601 tagttttaac aagcctaaat gagaaaggag catacttaat gaaaaatcta aagataatct
     6661 gtgacagttt atcggacgtg ccaaaagatt tgttagataa atatgatata gatatagttt
     6721 cattaacagt tatactggag gaaagagaat acaaggatag aattgatata tctggagatg
     6781 aattttataa aaggcttaga gaagaaaatg tatatccaaa aacttctcaa gctacttatg
     6841 cccaatttaa ggaaattttt gagaaatata caaatgagga tagagagata ctttatatag
     6901 cagggtctgc cacagctaca ggtacgtatc aaagtgctgt aatggcaaag aatgatgtag
     6961 atggtaaaat atatacatat gactctaact tgctttgtat gggaactggt ttacttgtaa
     7021 taaaagcagg agaacttgca agtgaaggaa agtctgttgc tgaaatattg cctgttttgg
     7081 atgaattaaa agagaaggct tttgttatat tttcagtaga tacattggaa tatttacaaa
     7141 agggaggtag aatatcatct actaaggcag caataggaag tatattaaat ataaaaccga
     7201 ttcttgaaat aagagatgga ttagtttcgc aaatatctca agtaagagga aagaagaatg
     7261 taatttctaa gatgatggaa ttgataaaat caaattgtgg tgatgattta tcagaccaaa
     7321 ttgtgtatat aggatatagt gatgatttta aggaaaagga acaattatct aaggttatgc
     7381 aagatgaatt aagacctaaa gaaattagat ttttccaaat tggtgtgtgt ataggtgcac
     7441 atgctggtcc tggtgttact ggtatcatat gcttaaaaaa taaataatat atttttggat
     7501 gacattattt atttttttat taaattagga tagaatatta attattgaga tgttaaattg
     7561 gatttatttt ggatgaaatt aagtgttgat atattagatt tagtttgtag ggaagtgtgt
     7621 aaatagataa gaatctattg cacacttttt tatagtaaga tttattaaaa aaatttcaaa
     7681 atgtacttgt aaattgtata taaaaaaatt
//
LOCUS       DAELNY010000173         7649 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.176, whole genome shotgun sequence.
ACCESSION   DAELNY010000173 DAELNY010000000
VERSION     DAELNY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.176"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..360)
                     /locus_tag="KPE55_003063"
     CDS             complement(<1..360)
                     /locus_tag="KPE55_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0494106.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
     gene            927..1289
                     /locus_tag="KPE55_003064"
     CDS             927..1289
                     /locus_tag="KPE55_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494107.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1273..1998
                     /locus_tag="KPE55_003065"
     CDS             1273..1998
                     /locus_tag="KPE55_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494108.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            2018..2437
                     /locus_tag="KPE55_003066"
     CDS             2018..2437
                     /locus_tag="KPE55_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494109.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2427..2951
                     /locus_tag="KPE55_003067"
     CDS             2427..2951
                     /locus_tag="KPE55_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494110.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3263..3820
                     /gene="efp"
                     /locus_tag="KPE55_003068"
     CDS             3263..3820
                     /gene="efp"
                     /locus_tag="KPE55_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG0494111.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3912..4367
                     /locus_tag="KPE55_003069"
     CDS             3912..4367
                     /locus_tag="KPE55_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0494112.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4530..5240
                     /locus_tag="KPE55_003070"
     CDS             4530..5240
                     /locus_tag="KPE55_003070"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG0494113.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5294..6364
                     /locus_tag="KPE55_003071"
     CDS             5294..6364
                     /locus_tag="KPE55_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0494114.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6392..>7649
                     /locus_tag="KPE55_003072"
     CDS             6392..>7649
                     /locus_tag="KPE55_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0494115.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENI"
BASE COUNT         3254 a          647 c         1371 g         2377 t
ORIGIN      
        1 ttctttatct gttccgatag ggtcttgtag agatacttga gttttatttt ttttgttaga
       61 tctaatattc attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt
      121 tcctttatct atgttataag tttctatagc tttaattaat cctattgtac ctatggatat
      181 taaatcatcc tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct
      241 catgtttctt tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt
      301 taaatactca atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa
      361 taggtcaccc ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga
      421 ctgttatcta ataaaataat atgcatattt ttagaattta aaacatatat ttgcaaactt
      481 ttgaatggaa tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat
      541 gaaataaggc gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa
      601 atataaaagg aaatagtatt tatatatgtt ttggtgttga agtaaaagat taatgcaaag
      661 cattagagtc tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta
      721 aatgtaatta attgtttgta taaagttgat tacaagcaaa agtatcagtc atagttaata
      781 ctgttaaggg tgaaggtggt gtgttaattt atatatgtat gggaagaaat tttgattaca
      841 atgaaaaaac ttacaaaaga tatttatgaa aaaacttata gttaaatgtt tatatataaa
      901 atattattta ggggttgttt ttattattga aaataagaaa gagtggtttt atatcaattg
      961 aatgtataat aagtattgct atattatatg tggctgttta tttagtttct acatcattgt
     1021 ataattgtta tagttttatc agtagaaata tatctgacag agaaatgtta agtacagcaa
     1081 aaaaatatat agaagatgag aagtatagaa tacaaaatag taagtatgag ttaattgaag
     1141 ataagataga aaaaaattac ataaatggat atgaaattaa cagtaaaata gagcaaattt
     1201 tagattatta tcaatgctat gaaataaata tagagataaa aaatgaattt aaaaaactga
     1261 ggtttaatag ctatgttact agaaaataaa ggctctatat taataataac attgataata
     1321 ttttctatta taagtactgt gtgcattatg tgtataggtt taatttatac acataataat
     1381 atgtttgagt taggatacaa ggacattcaa ctaaaagaaa tttctttaag tggaatagaa
     1441 atatcagtaa gtaatgtact ttcttgtgtt gaggaagcta ttgatgtatc taataatgaa
     1501 gacgaattta gagaatattt cttaggaaat aatatgattg aatgtataaa aaaaataaaa
     1561 gatacatctt atagtagttt agaagaactt tctttaaaag tagaaaataa tactatttat
     1621 gacagaggag atttttttga atttgatata gaaagtaatg ttagagaaaa agaattttta
     1681 aaagcaataa aagtaagagt gaaaatcaaa aatccatgga taggaattga cattgaagat
     1741 tatatggata aatctaataa caataaaatt gatgacaaaa cattcaataa ttataaagca
     1801 tctgaaaata atgaaattct aaatcaattt aaaaatgaaa gtgaaaatat aaggaatcaa
     1861 gaggttggtg gatttacaga agaagatgaa gaagatgaag aatatgaaga agaacttaaa
     1921 caaaacataa ttgaaaatat aaagaagaat tttgatgaaa aaaacctagt acaggtatat
     1981 aagtataggg gtgtataact ttgaaaaaag aaggaatgtg actgtaatag aattaatagt
     2041 atctatcagt ataatactct tgatagttac attatctttt ccaaaagata atcttgaaaa
     2101 tcataaaata aatttatttg cacgtcaact ttgttcagat ataaggtatg ttaggcgaat
     2161 taatatgttt agcaactaca atacatatat atattatata aatcaaggta aacacgaagg
     2221 ttatgtattg agaaaagatg gtaaaaacat taaatcaata atgctaccta aaaatgcaaa
     2281 aatagctagg agtattgaaa taataaagtt taaatctgat gggtcgcctc aaaaggcgtc
     2341 tacaatagag atatataata atagattaag aaaaactata acaataacac ctgtaagtgg
     2401 aagagtactt ctgaaagagg gaaaatatga aacataaata tggttacttg cttttggaaa
     2461 gtgtcgtatc attgtcttca atggtgataa ttatactagt tttatattct atatttttgt
     2521 ctacaataaa cttaaagtta aaagtagaag ataagataga actacaacaa caaagtcttg
     2581 aaatcataaa atctatggaa ggaattatta gcaattctat gggaataatg aatgtatcta
     2641 attatgaaga aacatttaaa aagacaacat ctataaaatg tagatattta gatgaaaata
     2701 ataatgaaga aggcataagt aataaagaaa ttatattgaa tgaaagacga aataagttat
     2761 ttgtaaattc tttaaatggc gaaagtagtc aagcaggtgg ttatgaaatt ggagattatg
     2821 tagatgagat gtatgtatca attactaata atggtcaata tgttaatatt aaattgaaat
     2881 tatcaaaacg tagtcaaaaa tatgagactg atttcaaaat aaaagtatgg aattttagtg
     2941 aaagtatata gaaatacata aatttaagag aaagaagtga catcaataag tcatataaaa
     3001 tataattatt gagaactata taactaaata atatcaatat gtaaagtgta tagattaaga
     3061 atatagagga taataataaa aatatagaat aagtaatttg ccaaatactt gtttatattg
     3121 tataacttat gatattatga tattagtaaa aatttgtaat cctgacttga cagaattcaa
     3181 gtaagagata taattttaaa ggcatagtaa tgtaaaattt actaaaaaat aagaaattta
     3241 ttttaatatg gaggaacaaa aaatggtatc agcaggtgat tttagaaaag gtgttacatt
     3301 tgaaaaagat ggacaaccat gtttagtagt tgattttcaa cacgttaagc caggtaaagg
     3361 agctgctttc gttagaacta aatacaaaaa cttaaaaaca ggagctataa gagaagaaag
     3421 tttcaatcca agtgaaaaat tccctaaagc tgttatagat acaagacaaa tgcaatatct
     3481 atacaatgat ggtgagttat attattttat ggatcaagaa aattttgaac aaataccatt
     3541 aaactatgag caagttgaag atgcaattaa gttcttaaaa gaaaatgaag ttgctacaat
     3601 aagattttac caaggacaac cattccaagt ggaagcgcca aactttgcag agctagaagt
     3661 tacagataca gagccaggta taaaaggtga tacagcaagt aatgtaacta aagcagctac
     3721 agttgaaact ggagcagttg ttcaagttcc attatttata aatactggag ataaagtaaa
     3781 gatagatact agaacaggtg aatatttatc aagagtataa aaatttatgt atataaaagc
     3841 ttatatgtct taataatata agatatatga gcttttagtt tatatataaa aaagaaagcg
     3901 aggggtgtta catgaaacat ttatatgaag aagttgcata cttaaaaggc ctagcagaag
     3961 gactagaaat aagtgctgaa agtaaagaag ggaaaatgat acataaaata gttgatgcat
     4021 tagaagtatt tgcagatgct attgtaacat tggatgaaga acaagaagaa cttcaagatt
     4081 ttgttgaatc tatagatgaa gatttagcag atttagaaga agatattgct gacatggaag
     4141 aagatcttta tgaagaagat gacgatgagg atgatgagga cttcagctat atagaaatgg
     4201 aatgcccaaa ttgtggagag ttagtagaaa tagacgaaga tttgttatat gatgatgaag
     4261 tagatgttgt ttgtcctgat tgtaaggctg tgatattatc atcagaagat gattgtgatg
     4321 atgattgtac atgtggtggt tgttcaagtt gtgatgatag agagtaaaaa ttcataagaa
     4381 ttaaaactgc ctatgatgcc tatgagatat tttgcttcat aggtagtttt atttttatga
     4441 ggaagggtat tatgtatttt ggttttgcaa tacttggaca aaaatagtac actagaattt
     4501 atagaatagg attttggagg gatgtaaata tgaaaataag taaaaaatta ctagataata
     4561 tacaaagatt tgaaaatgtt ataaattata aatttaaaaa taaagaatat atactagaag
     4621 ctcttactca tagttcatat tctaatgaaa ataaaaaata taattttaac gaaagattgg
     4681 aatttttagg ggattcggtt ttaggcatag tgataagtga ttatttattt aatgaagaag
     4741 ctaatctgcc cgaaggggaa ctaactaaac ttagagcaaa tatagtttgt gaagattcat
     4801 taagtgaagt agctaatgat ataaatttag gtattcacat gttattaggt agaggagaag
     4861 aagcaacagg aggaagacat aggacttcaa tattagcaga cgcatttgaa gctgtaatag
     4921 ctgctatata tcttgatgga ggttttgaaa gtgcaagaca gtttatactt catcatatgg
     4981 aaaatataat ttatgattca agaaaaggaa atatatttag agactacaaa acgcatcttc
     5041 aagaggtgtt acaagggaat ggagaaaata atatctggta caggcttata gaagaaaaag
     5101 gaccagacca taataaaaga tttgtaatgg aagttggaat taatgacgat gtacttggta
     5161 taggagaagg caagagtaaa aaagaagctg aacagttagc tgctaaaata gctttaaaga
     5221 aaaaattatg ggaaaaataa atataatatg taaaaatata aaaagaaata tttatattag
     5281 aaggatgttg tagatgaaaa agagaattat acctatattt gttcctcaca aaggatgtcc
     5341 tcatgattgc atattttgta atcaaaaaaa aataacaggt gtgagtactg atgttactag
     5401 tgaagatgca agaaatataa ttgaggaatg tttagagact atagataaag atgcagatgt
     5461 agaaatagca ttttttggtg gtagctttac tgctatagat attgatatac aaaaaagttt
     5521 attatcagtt gctaaagaat atgtagaaaa aggtcttgta aaagatatta gaatgtctac
     5581 aagaccagat tgtattgatg aagatatatt aagtatgtta aaagaatata agacaagtat
     5641 aattgaacta ggagtacaat ccttagatga aaaagtatta ttagatagta taagaggtca
     5701 tcaatctgaa attgtataca agagttcaaa gatgataaaa aatagtggaa tcaaacttgg
     5761 attacagatg atgattggtc ttccagctga cacagaagaa aagtgtatat ttacagcaaa
     5821 aaaatttgta gagctaaaac cagattgtgt tagagtctat ccaactttgg tagtaaaaga
     5881 tacaggtctt gaaaagttaa tggaacaaaa taaatataat ccatttactc ttgaagagag
     5941 tgtaaatata gtaaaaaaag ttcttgtact gttttatgtt aataatatca atgtaataag
     6001 agttgggctt caagctactg atgatataca aattggaaaa gctgttttag caggaccata
     6061 tcatcctgca tttagagagt tggtagaagc agatatgata aaagattatt tagaatttgt
     6121 tattttgcaa aataaaaata taaagcaaat gttggtcaaa tctaataaaa agaatatttc
     6181 taaaatcata ggaaataaaa aaacaaatgt taaatatatg gaagaaaaat ttggtgtgct
     6241 tttaaaaaca caagaatcag atttagatat aaatcaatta gaaattgtgt tagatgggaa
     6301 aagtttaatt agtgctaata tgagagacat tcatagaaaa ttgtatgaca tttatgacct
     6361 ttagttttaa taatgaaggg agagaaaaga tttgtattta aaaagactag agttaaaggg
     6421 atttaaatcc tttccagtaa aaacagatat tatttttaaa gaaggaataa cagccatagt
     6481 aggaccaaat gggagtggaa aaagtaatat atctgatgct gtaagatggg tattaggaga
     6541 acagagtatt aagagtctta gaggtgataa actcgaagat gtaatatttg caggtacaga
     6601 tacaaaaaaa cctatgaatt attgtgaagt ggctcttact attgataatt ctgaaaatca
     6661 attggaatta gattttacag aagtaactat aagaagaagg gcttatagaa atggtgaaag
     6721 tgaatttttt ctaaataata aaagttgtag attaaaagat ataaaagaag tctttttaga
     6781 tactggtatt ggaaaagatg gttattcaat catagaacaa ggaaaagtag atgaaatatt
     6841 aagtaataat cctttaagca gaagaaaagt ttttgatgaa gcttgtggca tatcaaaata
     6901 tagatataaa aaacaggaag cagaaaggaa tttaagtaat acaaaggaaa atttagaaag
     6961 aatagatgat gtatatatag aaattgaaaa tcaattaaag cctcttttta atcaacagac
     7021 aaaggcaaaa aagtacttgg aaataagtga aaaattaaag acacttgaag taaatagctt
     7081 tataagagaa attgaaggaa tagaaaaaga attaagtgag gttaatgaac atcgtaaggt
     7141 tattgaaaag gagctcaatg aaaaagaaga acaaaaaaat gttgtagaga aaaagcaaga
     7201 agatattaat aaagaggtag aggtattaca agatgtaatt gaaaaatctg tagattatat
     7261 aaattcaatt aaaggagtta tatcaaaaaa agagtcccaa ataaatttaa ttaaggaaag
     7321 aataagaaat tttacgaatg aaattagtag aaaaaattta gaaataaagg atataaaaga
     7381 gaagctaaat gaaaataagc aatacataaa agaattagaa agcaataaat tatcaggtag
     7441 tgaagaacta tctaccttgc aggaaaatat aaaagtattg gaaggaagta aagataaaca
     7501 aaaaattaag ttagaatccc taaataatga gatagaatta ttgaaagaaa gtataattga
     7561 tatattaaat aagaaacaag agtttagtaa taagctgtct actttaaacg caaataaaga
     7621 aaatatgaat atcagagatg aaaatataa
//
LOCUS       DAELNY010000174         7597 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.177, whole genome shotgun sequence.
ACCESSION   DAELNY010000174 DAELNY010000000
VERSION     DAELNY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.177"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..1519
                     /locus_tag="KPE55_003073"
     CDS             116..1519
                     /locus_tag="KPE55_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0494116.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1610..1850
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1893..2591
                     /locus_tag="KPE55_003074"
     CDS             1893..2591
                     /locus_tag="KPE55_003074"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG0494117.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2614..3615
                     /gene="trpS"
                     /locus_tag="KPE55_003075"
     CDS             2614..3615
                     /gene="trpS"
                     /locus_tag="KPE55_003075"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG0494118.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4267..5834)
                     /locus_tag="KPE55_003076"
                     /pseudo
     CDS             complement(4267..5834)
                     /locus_tag="KPE55_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5809..5952)
                     /locus_tag="KPE55_003077"
     CDS             complement(5809..5952)
                     /locus_tag="KPE55_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494119.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(6170..6352)
                     /locus_tag="KPE55_003078"
     CDS             complement(6170..6352)
                     /locus_tag="KPE55_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494120.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(6741..7487)
                     /gene="cdtR"
                     /locus_tag="KPE55_003079"
     CDS             complement(6741..7487)
                     /gene="cdtR"
                     /locus_tag="KPE55_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG0494121.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         2713 a          911 c         1114 g         2859 t
ORIGIN      
        1 ctgtttttga atatgcaaat attttgaaaa atatcagttt aataataatg aatctatttt
       61 attaattttg aaattaaatt taaaataatt aatagagagg ggaaattaaa ttaggatgga
      121 aaagaaaaaa ataggcctat ggagtttggt atttatgaat gtatcagctt tatatggtat
      181 aagatggata gctaaatcta cagcatcaag ctttggttta ggattaggtg caatacctat
      241 gtggttatta ttctcattta tatactttgt accaggagct ttaatttgtg ctgaattagc
      301 ttctacatat caaaaaggtg atggaggtct ttacgattgg gttaaggaag cctttggaga
      361 aaagtatgga tttttagttt cttggttaaa ctggacagct aaaatatttt ggtattcttc
      421 atttttaact ttcttagcag taaatgtttc ttatacaata ggaaatccag cccttgctga
      481 taataagatg tttgtactta tattatcttt aataatattc tgggcattat ctcttatagc
      541 aactaaagga ataagtttta caaaaatatt tactaataca ggtgcattag gttcaacaat
      601 accaagtgct atacttataa tattttcatt tgtagcagta tttgtgttga aaaaacatga
      661 tatagcttct acatacacaa tagcttcaat gactccaaaa cttaatatgg atgcatttgt
      721 tgctatatca gctataatgt ttggtttagc aggagcagag actgctgcta acttcataac
      781 agaaatagat aagcctgaaa agaacttccc taaggcaata ttaatatcag ctggaatagt
      841 agcttcatta tatgtattag gctcaattgc aataacaatg ataataccac cagaccaaat
      901 tactgcttct aaaggtgtat tagatgcact ttctgctgtt tgtgcgtctt taggaattgg
      961 ttcttggttg gttcaattaa tagcttttgg tatagctttt tcagtattag gagcaatagt
     1021 tctttacata gcatcaccaa taaaaatgtt atttggtagt gttaaaaaag gaatatttcc
     1081 agatagttta gtagaggtaa atgaacacaa gataccttct aaagcagtta tacttcaagc
     1141 aataatagtt actataatac tattggtgac tacattaatg ccttctgttg atgctatata
     1201 caatgttctt gtaactatga cagcattaac tgcattattc ccatatgtat tgttatatgc
     1261 ttcttatatt aaactaagaa aagagagacc agatgaaatt agaccttata ctatggcaaa
     1321 atcaacaagt acttgcctag gtttagcaaa aatggtttta gttgtaactg tagtgggtat
     1381 attattatct gcagcaccag ttatgcctac atttgctgaa aatgttatat atgagataga
     1441 gatgattggt ggaggtcttc tagttatatt atctggtcta tggttatgga atagatatga
     1501 aaagaaaaca gctaaataaa atgtatttta aaaatttctt ttggatatta taatatatag
     1561 ttgacaattt atagtgtaag gaatattata aaataaagaa attaataaat taaatcctga
     1621 gataagaaat agtaaataaa tatatttttg cacagagaat taaggatgct gagaacttaa
     1681 taaaaagtat ttattgaatg ggtctttgag gagtaaaatg aaatgtaatt taatattaca
     1741 gagtatttga tatcgtgtat gcacgttata gcatatatta agatatatat attcaatttt
     1801 gagtgtatat aaatttaggt ggtaccacgg aaaatatatc cgtcctattg atttaggacg
     1861 gattttttaa tatagaaaat ttaggaggaa aaatgaacgt aataggaatt ataggtgcaa
     1921 tggatgaaga agttagtatt ttagttgatt taatggatat tagagagact ataaaaaaag
     1981 ctagcttaga attttataag ggaattttgg aaggtaagaa tgtagtgtta gtaaagtgtg
     2041 gcataggtaa ggttaactct gctctttgtg cccagatact aataagcgaa tttaaggttg
     2101 atgctattgt taatacaggt gttgcaggag ctttaaacga aaaacttgat gttaatgata
     2161 tagtaatatc aactgatgct atacagtatg atgtagacac tacagcattt ggagacccta
     2221 agggagttat accaagaatg aagacttctg tatttaaagc agatgaaaga cttatagatg
     2281 ctgcttacaa atcttctgtg gaagaagtaa agactcataa agtattaaaa ggaagagtag
     2341 ttacaggaga taaatttata aattctaaag aattaaaaga agaattagta aatgattttg
     2401 gtggatattg tggagaaatg gaaggtggag ctatagctca tgtatgctat ttaaataata
     2461 ctccatttgt aataataaga gcaatgtctg ataaggcaga tggaagtgct gatgttactt
     2521 atgatgtatt tgtacatgat gctgccaata actctaaaga catagtttta aatatgttaa
     2581 aatcaatata aatatataag gaggtacaaa aaaatgagtg aaaaaaaagt tatatttagt
     2641 ggtgcacagc catcaggcaa aatgacttta ggaaattatt taggagctat aaaaaactgg
     2701 actgaacttc aagacaatta tgattgttat tatagtatag ttgatttgca tgcaataaca
     2761 gtacctcaag accctaaagt tttaagagcc aatactatag aattacttgc tcaatatata
     2821 gcttgtggat tagacccaga aaaaaatact atatttatac aatcacatgt caaagagcat
     2881 gttgaactta tgtgggtatt gaatactatg acttatatgg gtgaattaag tagaatgact
     2941 caattcaaag ataaatcaca aaagagtgaa gcaaatctaa atgcaggttt attcacatat
     3001 ccagttctta tggctgctga catattgttg tatcaaactg acctagttcc agttggagat
     3061 gaccaaaaac aacatttaga attagctcgt gatttagcaa atagatttaa taatagattt
     3121 tcacctactt ttgtagttcc agaaggatac tacccaaaag gtggagcaag agtgatgagt
     3181 ttgcaagagc caactaaaaa aatgagtaaa tccgattcaa atgaaaatgc atttatacta
     3241 ttagcagatg attcagattc tataaagaga aagataaaaa gaagtgttac tgattcttta
     3301 ggtgttgtaa aatacaatga tgaacaacca ggtattaaaa atctaattga tatatattct
     3361 aatttaagta aaaagagtgt agaagagatt gtaaacatgt atgaaggaaa gggttatggc
     3421 atatttaaag aagatgtggc tgaagtagta tcagaagctt taagacctat aagagaaaaa
     3481 tatgttgacc ttcttaataa taaagattat ttagaaaaaa tatatagtat gggagcagaa
     3541 aaagccgaaa aacaagctag aaaaactctt agaaaagttt ataaaaaagt tggatttata
     3601 gagagaagat actaattatt agctatcttt acttgataaa taaattttaa agttattaaa
     3661 aaggtatatg atatgaaaga gagagtctca aagtgaactt ttgttcatga ggcactcttt
     3721 tttgtatgaa ataaaatata ttttaatcat ttcaacaatg aaaaagagga ttatctctat
     3781 tttgagacag catcttttga taataaaaaa agatgagctt ttgctcatct caaaggggtt
     3841 agattattct tactttgggg aaagtagaat aaaggtaaaa gttaactgat gtcaactttg
     3901 tcttttttgc atatcactac tgagcataaa aaaatcagaa aaccatagcc ttctgaaaca
     3961 acttctaaca tacattgtat tttataccca atatgtaatt tatgagttaa ttttaactct
     4021 tataatgaaa attgtcaata atatatcaaa aaaatatttt ttaaatttat ataattaaat
     4081 gaatttatgt ttatatatag gtgatatgat acagataatt atctttttaa tacaatatag
     4141 ttctaaaaaa gaatatatta gaagaagata atatttaaat aaaaaatacc ttataaagtt
     4201 aattttttaa tactttataa ggtaaataca tattgtattg tggggataaa tttaaatcat
     4261 ttgatactaa tcaacactaa gaaccaataa ctctctatca tcttgagtaa ttgtatatat
     4321 tcttaatttc ttgtatgtca taatattttc tccacctgta aaataactcc taagattaac
     4381 acaatcagtt ttaggttgat ttgtatctcc taaataatta cgcaattctt tatctttagt
     4441 ttcaatatct ttaaatccaa aatattgtaa agtagcttca actctatatt tttggacata
     4501 atcgagagct tctttattag tttatgttgg tgtaaaatac ataccattta tataagcctt
     4561 tattgtactt ggattaaatt taaaatctgc agaatatatt ttatgtgtaa ctattatttc
     4621 ttgaacagtt ggaattttaa cttctggttc atcaagtatt tcaggagtac tatttagctc
     4681 tgtaatagat aagttatcta agtatattgt atcactatca attaatgcta tatttatatt
     4741 agaagaatct ttttcagtag tttcaaattc ataactaaat tttgtatatc cttgttctag
     4801 taccaaataa tccgtttttt cttcttttac ttttatattt acaattattg aattatatgt
     4861 taaaggattt tttgaatttt actaaaaaca taacgtttat aaggttttag ttcagacata
     4921 ggtattttaa tcttcgtctc accatttaat ctatttgctg agccttttaa accatcttga
     4981 tttgtagtat taacattact ccatatacta gggtaattat tgtaatcatc aaaattagta
     5041 aagtaagttg gtgtttttat aagtatattc attcctcttt caagtttaac attacatatt
     5101 tgtttatcag acaaagtttt taaactgtct ttaatcttat tgcctatatt atcatcaaat
     5161 atgagttcaa cacaactttc atctattggt atattattaa aatataacaa accatctttt
     5221 ttagtagtgc caaaagtttt ttcaattgct tctccaattg taagttcagg tgttttatct
     5281 tctgggacct gtaaactttt agcagtaatt cttcttttgt aagattcatt ttctgtatct
     5341 aatataatag atacagaaat actgtcaatt tgacctatat cgtcttacca actatttcat
     5401 tctgttacta tttgtccaga accatttttt gtaccaaaat ttccacttac ttgtgttgtt
     5461 tctaatttaa tttgctttcc atcatctaat ttttttaatt aattataatt tataggaatc
     5521 agtctagaac taaattgata cattgtgtta agagccatag gtgaaaatcc ttttttgaga
     5581 taagtatttc caggagatag attattgcca atttgatttt cttgtgcttt gatagttgat
     5641 aatgcatctc catctaacac taaatttgtt gttggtgtca ctttgtacat aggtgcagta
     5701 cctgtgttgt agtatctaat atttgcattt atatatgcag attctccttt gtttatactt
     5761 aatccagtat ttcatgatta tccattgcta ttttgaacag cagttgaatt atctgttgta
     5821 tggtaataat ttgtcttgta tagtttcttt aaactcatta aatttttcta atgaaatttc
     5881 gttttgattt tttgttctta ttactttatt aaatgcaaat ttttctggag attcaaaata
     5941 atatacatac ataggtttat ctattttatt ttttgatata gcatttcgaa gttctctata
     6001 ttctttttct caagaatttg cttctatttg ataatcataa aaataatttc ttgtctgaga
     6061 atatttgctt atctctatag aatctttttt atatgatcct aatacttctt tttcagattt
     6121 ttcaagtttt tgatcttttc tttctcattc tttagccttt tctttatctt taaaaaaatc
     6181 cttctgatct ttctatagga gccttgtaag tagtattaca aacttttcca ctatagcttt
     6241 gtaaatcttg tgcataaatg ttattaggta acaaactacc taaagttaga tataatatca
     6301 acaatataga tatacaatta ctaatacttt tatgtttcct aaattttttc atttattctc
     6361 cctacaaata ttagtttagt taacttttga attttaattt atttagatta tataatctaa
     6421 ataagaattt tttatataaa tttcataaaa tatgtaggta tatatcataa aaatatgtat
     6481 ttatatgata tgtcagtaca aaataacaac tgaatatttt aaagagacta tttcgtactt
     6541 ttatgattta atgagctatc ttatcgaatc ataatctagg aacaattgtt ataatatcta
     6601 atagtagtta tatatgatat agtcagtatg tgttttttca caatatgatt attatataga
     6661 ggattcttag ggattactct ttttgtataa aaaagagatg gttcaaattt ttatcttgag
     6721 acatctcctt tttctattta ttatgtttta ataatattct ttaaaatatt tctatatgta
     6781 ttagatatac gacatatatg gccattactc attttaatat tatttttctt taggtcgaga
     6841 gaaacaatat gatatttatt aactacaaaa gatttatgta cacggataaa gcatgcatct
     6901 aaatctggta aagactttaa acttttatag aattcaaata ttctagaagt agtcactaat
     6961 tttattttat gtggaatagt gcttgtttca aaatatataa cttcatcaaa tgaaattttc
     7021 catagtgaga aatctgaata gatagtaaaa aaattagagc gataattttt ttctttaatg
     7081 tatcttttgt gagcaatttt aatacaagct tttattttac tttcagcaat ttccatatca
     7141 tgttttagta taaagtccat aacttctaac ctataatcga agatccctaa aatcatttct
     7201 atatgatttg taagaaatat gattggacta atataatctg attctctaat tttttgtgct
     7261 atccataatc catcacataa ttcattactt atcttatatt ttgcgtctac aactataaaa
     7321 tatattctta ctttattttt ttcagtaagc tcttttaata acaaatttgc attatgatat
     7381 aatcttacaa catcattatc aaaaccttct tttattaaaa tattatttat tttttctttt
     7441 agtttcattc caaaacaaac atcattatca aagattaata tatccactaa ataccctcct
     7501 ataaaaaatt caaaagtaaa aaaacaaaag tattttatag aaaatacata aaataacact
     7561 aggagataaa taagattaag gatagttaga attaagg
//
LOCUS       DAELNY010000175         7581 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.178, whole genome shotgun sequence.
ACCESSION   DAELNY010000175 DAELNY010000000
VERSION     DAELNY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.178"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..268
                     /locus_tag="KPE55_003080"
     CDS             <1..268
                     /locus_tag="KPE55_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0494122.1"
                     /translation="TGYTSNIVRRMNEHKLGINSKYTRAKGFEKLEVYFVTNTKSNAM
                     KLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQLT"
     regulatory      361..453
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            553..1347
                     /gene="thiD"
                     /locus_tag="KPE55_003081"
     CDS             553..1347
                     /gene="thiD"
                     /locus_tag="KPE55_003081"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG0494123.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1371..2159
                     /gene="thiM"
                     /locus_tag="KPE55_003082"
     CDS             1371..2159
                     /gene="thiM"
                     /locus_tag="KPE55_003082"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG0494124.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVKGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2182..2814
                     /gene="thiE"
                     /locus_tag="KPE55_003083"
     CDS             2182..2814
                     /gene="thiE"
                     /locus_tag="KPE55_003083"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0494125.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            2953..3255
                     /locus_tag="KPE55_003084"
     CDS             2953..3255
                     /locus_tag="KPE55_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0494126.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3321..4076)
                     /locus_tag="KPE55_003085"
     CDS             complement(3321..4076)
                     /locus_tag="KPE55_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494127.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4064..5026)
                     /locus_tag="KPE55_003086"
     CDS             complement(4064..5026)
                     /locus_tag="KPE55_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494128.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNALSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5030..5488)
                     /locus_tag="KPE55_003087"
     CDS             complement(5030..5488)
                     /locus_tag="KPE55_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0494129.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNFPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5843..6199
                     /locus_tag="KPE55_003088"
     CDS             5843..6199
                     /locus_tag="KPE55_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0494130.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6271..6987
                     /locus_tag="KPE55_003089"
     CDS             6271..6987
                     /locus_tag="KPE55_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494131.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            6980..>7581
                     /locus_tag="KPE55_003090"
     CDS             6980..>7581
                     /locus_tag="KPE55_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494132.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFS"
BASE COUNT         2951 a          889 c         1243 g         2498 t
ORIGIN      
        1 cactggatat accagtaata tagttagaag aatgaatgaa cataaattag gaattaattc
       61 aaaatacaca agagcaaagg gatttgaaaa actagaagtt tattttgtga ccaacacaaa
      121 aagcaatgct atgaagttag aatactatat aaaaaaatta actagaaata aaaaattgtc
      181 tataataaaa aacccaagta tacttataaa tttaattgat aataaagaag attatattat
      241 tggtaaggaa atagaacaat taacataaat atttatagta acattataat gggtgcatat
      301 ggtgtaaaaa cttgacaata aatactattg taatataatg aagttataaa tatagtaaat
      361 taaaatagct aggggagcca agagctgaga ggtataccga cccttacacc tgatctggat
      421 aataccagcg taggaaagct tagtaaaaat tcaagtatgt attatttgtg atatatgcta
      481 tttctaagct atacctattg taggtatagc ttttttaatt aagttattta aattaaactt
      541 taggaggcaa caatgagtaa ttataaaata ccaacactta caatagccgg ctctgattca
      601 tctggaggag ctgggattca ggctgatttg aaaacgttta gtgcaattgg tacttatggt
      661 atgagtgtaa taacagctat aacagcacag aatactaaag gagtttttgc tgtagaggat
      721 ttaaataaga aaataataaa aaaacaaata gaggcagtct ttgaagatat acctccaaga
      781 gctgtaaaaa taggaatggt ttcaagtcct gagattatac ttgaaatagt tgagaatcta
      841 aagaaataca atccaaaata tttggttgtg gacccagtaa tgatatctaa aagtggatat
      901 tatctattaa agccagaggc aaaagaaaac ttaattaagt atttgatacc acttgcatac
      961 ataattacac caaatatacc agaggcagaa gaaataacag gaataaaaat acataatgta
     1021 gatgatatga agagagtagg agaagaaata ctacaacttg gacctaaatt tgtacttatg
     1081 aaaggaggtc atttagatgg agaagcagtg gatattttag ttggtaaaaa tatatttaaa
     1141 gtttataaaa gtgaaagaat tgataaaaaa aatacacatg gaacagggtg cacattatct
     1201 tctgcaataa catcttatct agcattagga tatgaaataa cagaagcagt aaatctaagt
     1261 aaaatctata taacagaagc aataaaaaga agttttgata taggtcatgg agtaggacca
     1321 gtacatcatt tttataaatt tgagtaataa aaaaatattg gaggtagaat atgtataatt
     1381 taattaaaga tgtaaaaaaa ttaaatccat tggtaattca ttatactaat aatgtaacta
     1441 taaatgattg tgctaatgta actttagctg taggagcaag tcctcttatg agtttttcat
     1501 atgaggaagt agaagaaatg gtaagtgtag caaactctgt agtaataaat ataggaacta
     1561 tgaactcaaa tatgttggat ttattcctat tagctggaaa agcagcaaat aagtacaata
     1621 aacctgttgt tttggaccca gttggagtat ttgcaagcaa agcgagagca gaacttacaa
     1681 gtagactttt aaatgaagtc aaatttagtg ttgttaaagg gaatgtatca gaaataaagt
     1741 ttataggtgg ctttaatgta aagggtaaag gtgtagattc atttgatgaa gaagaagatt
     1801 ctactgagat aataagaaag attgctgaaa agcttgaatg tgtagttgta gcaactggaa
     1861 aaatagatat aataactaat ggtaaaggta catataaaat aaacaatgga actgataaat
     1921 taaaaggaat tacaggaact ggatgtatga ctgctagctt gattgcaagt tttatggctg
     1981 ttactgagaa tatattagaa gcagcaacaa tgggtgttct tacaatgtct ttaagtgggg
     2041 aattagctaa tttaaataat ccaccaatag gtacatttaa agaaaatctt atgaatgcta
     2101 tttatcaaat ggatatagat actttaagta aaaattctaa tattgaattt ttaaattaat
     2161 aaagagtaag ggagacttaa aatgattgat aaagaatctt taaaaaaatg tttaaagttg
     2221 tatttagtaa ctgactctga aatgcttaaa ggtagagact tttataaatg tcttgaagat
     2281 gcaatatctt ctggaataac aactgtacaa cttagagaaa aaaatgcttc tggaagagag
     2341 tttttaagaa aagctatgaa acttagagaa ataactaaaa gatatggtgt taagtttata
     2401 ataaatgata gagtggatat agcacttatt tgtgatgcag atggagtaca tgtaggacag
     2461 agtgacattg atgttagaga ggttagaaag cttataggaa acaataaaat tctaggagta
     2521 tcagcaagaa ctttagaaga agctatttgt gcaaaaaatg atggcgcaga ctatttaggt
     2581 gtagggtcta tattcacaac atctactaaa ttagatgcaa aatcagcttc gtttgaaact
     2641 gttaaagaaa ttaaagaaaa agtagacatg ccatttgtac taattggagg tataaattta
     2701 gacaatatag ataaacttaa atgtttagaa agtgacgggt atgctataat atcagctata
     2761 ttaaaagctg aagatatctc taaagaagtg gaaaagtgga cattaaaaat ataaaattgg
     2821 ctctggtagt gaaaaaaatg gaaaaatttg atataatcta agagtaaaat ttaagcaatt
     2881 ataaatgata aacttttcta catatacttt ttaatcaaga tatatgtaga tttggttaaa
     2941 aaggagctat aaatgattag gatattgtta gtgtgtgttg gaggcatgtc ttctacccta
     3001 ttggtaaata aaatggagaa ggatgcaaaa aaaagaaata tagactgtaa aatatgggca
     3061 gttggagaag gagatataaa atcggagctt gataactttg atattttact tcttggacca
     3121 caacttagat ttatgcttga tgacgtaaag tctatagttg gagatagagc tccagtatct
     3181 ataatagata tggtaaacta tggcacttgt aatggtcatg ctgtgttaaa ttcggtttta
     3241 gaaatattaa aataaataat aaaaatggat tctatataat agagtccatt tttattattt
     3301 gttattaata ggcttaaaac ttagcttacc atttcttttt ttaatctcca catagcaagt
     3361 gaaaaacttg aacagcataa tattaaaaat agtaggataa caggcattat atctccaata
     3421 agaatagtct caccaaatat taagctacgt aaagcgttaa taacatgggt aaatggattg
     3481 agcatcactg ctacttttaa tcctccagat aaactttcta tagggaaaag agctgaactt
     3541 aaaaagaaaa taggaagtac aattgcagtc ataattgttt cataaactat ttcatttggc
     3601 aataataaac ttatagaata agctagactt gataaaaaga aggctgtcat aaatatcagt
     3661 actatcataa gtaggattcc accaatgcca gattctatcc ttactgagaa gaatatactt
     3721 actatacaca aaatagttac ttctatgaaa gaaactaaga tagcttctaa taattgacca
     3781 aggacgattg aatacctact gataggtgca attaagactc tatagaaact tccactgttc
     3841 ttcattataa agtttataat accaccacta ctacaagaag aaaacacaac cagtacaatg
     3901 atacctggta gtataaaagc agtataatta ttgatattta tattattcat agtttggttt
     3961 gcaatagaac tataaagcac aagccaaaga aaaggttgta atatagtaac tacaaatgag
     4021 attggatttt taaaacgcca cttcatacta cgccataaga tatttaacat tcccattcct
     4081 cctttaagct ttttttagaa cttgtaagtg ctaaaaatac atcttcgaga cttggctcta
     4141 caatttctat tgcatcaaat tctatatcat gttctaaaag ccatttattt attaaagtaa
     4201 aagcagttct actatcgttt acacttataa aaattgaatt ttctcgtacg ctcataaact
     4261 ttactaaacc agtactttta attgcatctt tatatttttt tatatcttca gtattatgga
     4321 aagttatacg tagtatattt tgtctaatgt aactacgtaa acttgatggt gttccttgtg
     4381 ctaaatcctt tccattcttc ataatacaaa tattatcact caattgttca gcttcttcaa
     4441 gataatgagt agttagaaat atagttgtgc catactcatc tctaattttt aataacatat
     4501 cccacataga ttttcttgaa tcgacatcca taccaacagt tggttcatct aaaaatagga
     4561 ttttaggaga tgataccata ttcatagcta tgtccaatct acgttttaca ccccctgaat
     4621 atgatgaagt aggatatttt aagtaactag ataagtcaaa cttatcaatt aaagagtcta
     4681 ttctttgttt tgcaatctgt ggttctactt tatacatttt actttgaaat accatatttt
     4741 ccataaggga taaatgttca tcaatagaga tttgctgtgc tacacaagca atttgagtac
     4801 gtatccaaga aggattatct actaaatctt ttccaaacat tgttacattt ccagatgtag
     4861 gtttgtaaaa ggtagtcaaa atatttataa gagaagattt accagcacca ttaggaccaa
     4921 gtaaagaaaa aatttctcca gcatttacat taaagcttag agcatttaaa gctttcacac
     4981 catttttata ttcttttact aaattgtcta tttcaatcgc taacatatat tattcctcct
     5041 ctttaagtgg aaatattatc tctgtaatat agttatctgg gtttcctttt atcaacatac
     5101 ctggtccttt aataaatact tctctccaag gagtttgtat ttttatatta ttttcatgaa
     5161 tatatttaaa tatcatttca tacgtttttg gaaaattact ataagagcct gtgtgagtaa
     5221 aaaataaagc ttttgtttta ggaaattctt ttgtagttat acccatacga tttggagttt
     5281 cagcagttgg aacacataat tccatttctg caacaccagt tttttggtca acatcatagt
     5341 agcaaaagaa tggtgcaccg ttagactttc cttttatagc tttaaataca tttggaaaat
     5401 actttgttgc ctcagtcata ggtccattat aacgcatggt agccactgtg actgattcga
     5461 tatttttgat ttcaattttg caattcattg tttacctcct aatgtgtgtt catattctta
     5521 ctagattttt aagcattaat acatataaga ttttgattag ttgaaaacat ataatcttat
     5581 ctcgtttgct tgttttcaag tctaacataa cataaaatat atatcctctc atttgatgct
     5641 ccattttgta aagttattat ttaagcttta ctttagattt tttagcaaat taaaataaag
     5701 caatttataa aataagtatt tacaaattaa gtgtattaat cgtataatac acttaatagg
     5761 atactagata aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac tatcgtatta
     5821 atacactaga ataggaggaa tcatgaattt tgaattagat aatacaactc ccatttattt
     5881 gcaaatagta aagtatataa agagacaaat agtaacagga gagttgaaac caggagagac
     5941 aataccatca agaagagaaa tggcacttaa tctaaaagta aacttaaata cagttcaaag
     6001 agcatataag gagatgggtg atatgaatat aataaatact tttaaaaatt atcaaagcag
     6061 tgtaacagtt gatgaaaata tattaaaaaa tttaaaatta gagcttataa atgaatcact
     6121 atctgttttt atagaagata tgaaagcaat aaatgttagc aaagaagaag ttttaaaaat
     6181 aatagaagat aagtattagt tttttaacaa atagaaaact tagtttagaa agtttattta
     6241 taagcatatt ggatgtggag gatagtaaat atgatagaaa taaagaatgt cagcaaaaca
     6301 tataaaagga tgcaagggct taaaataaga aagatagaag ctttaaaaaa tgtaagtttt
     6361 aatatagaaa agggaaaaat aactgcatta cttggtataa atggtgttgg taaatcaact
     6421 atgttaaaag caatagcagg acttataaac attgatagtg gagaaattcg tatagatgga
     6481 gaaaaaataa atgaaaaggt atacaataaa ttggcttttg ttccagatgt acaatctcac
     6541 tttagcaata caacaataaa agaaacattt gaatttatgg aaatttttta ttcaaagtgg
     6601 aataaagaaa aatcaaaaga aatgatggac atatttaaat tggatgaaga tgaaataatt
     6661 gataacttgt caaaaggaaa cattgcaaga gtaaaattaa tacttggatt ttgccaagac
     6721 ccagagtaca tacttcttga tgaaccattt acaggaattg atttgtttaa gagggaagag
     6781 tttataggag taatagcgca atatatggag gaaaatcaag ccattatcat aactactcat
     6841 gagatagtag agatagaaag tttagttgat gaggttgtaa ttcttgatga agggcagatt
     6901 ataacttcat ttaatgcaga agaattaaga gaaagagaag gtaaatctat attagataag
     6961 atgagggagg tatataagaa tgaataaact tctatatcta ataaatgtag atttgagaag
     7021 aagcaataag ttttatatag cttatatatc atttttttca ctaattacac ttagtttaaa
     7081 tttattcgaa attaataaat ttaaaaattc taaatttatt atgaacttag caatagaaga
     7141 ttttggaggt atattttatg gactagggat acttagtaat cttggtttta tccaaagtat
     7201 attgttcttt gggatattgg gactttttat atactatatt tttatgtgga aaagagagtt
     7261 cttttcaaat aatcaaagta tttatacact tatgatgtta cctcaaaata agtttaagat
     7321 ttgtatatct aaatcaatag ctttgataac aatgatatat ggttttttga taagtcagac
     7381 agcaacatta tttatatgta aatatatatt taatttcatt tttagaaata tgcctataat
     7441 caatatgaac tttgccaaag atttatatta tttaaacttg aagagtatac caattgattt
     7501 tatctctttt atggctatct atgtattttt actacttata gctatttcta ttgtaaattg
     7561 ctgtatatta tttatctttt c
//
LOCUS       DAELNY010000176         7441 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.179, whole genome shotgun sequence.
ACCESSION   DAELNY010000176 DAELNY010000000
VERSION     DAELNY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.179"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(105..824)
                     /locus_tag="KPE55_003091"
     CDS             complement(105..824)
                     /locus_tag="KPE55_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0494133.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLKHLDYIIASL
                     HRPCIASGTKEKNTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(942..2216)
                     /locus_tag="KPE55_003092"
     CDS             complement(942..2216)
                     /locus_tag="KPE55_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG0494134.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAKLQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(2416..3783)
                     /locus_tag="KPE55_003093"
     CDS             complement(2416..3783)
                     /locus_tag="KPE55_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0494135.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(4033..4497)
                     /locus_tag="KPE55_003094"
     CDS             complement(4033..4497)
                     /locus_tag="KPE55_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG0494136.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(4502..5131)
                     /locus_tag="KPE55_003095"
     CDS             complement(4502..5131)
                     /locus_tag="KPE55_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0494137.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(5165..6052)
                     /locus_tag="KPE55_003096"
     CDS             complement(5165..6052)
                     /locus_tag="KPE55_003096"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG0494138.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(6081..7319)
                     /locus_tag="KPE55_003097"
     CDS             complement(6081..7319)
                     /locus_tag="KPE55_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494139.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
BASE COUNT         2583 a         1269 c          755 g         2834 t
ORIGIN      
        1 tgctaaaacc atatatatag tcattccttt tttaaaataa ccattgtcat aataattcat
       61 atagataaac ttatttttac tcaaattttt tcactcccaa gattctaaaa atttatatca
      121 aaaaattcta taatttcatc ttcatgataa tttatgacta attcatctgg aaaatctact
      181 gcttctaaaa ctttttctgc attttcaaat tctcctatag aataacatat atgagagtca
      241 gtaccaagaa ttactcttgc accatatgtt ttacacaatg tcaacatatg acttacattt
      301 tcccaagtac ccgttctatg agagtttgaa cttaatgatg aattatttat ttctaaaagg
      361 atatttttat ctttagcttt ttttactata ggttcataat ctaaaggata cctactgtca
      421 tctggatgtc caattatttt taccttaggt ttatccataa cttttaaaat agcatttgta
      481 tttttttctt ttgtaccaga ggctatacaa ggtctatgca aacttgcgat tatataatcc
      541 aaatgtttca acatatcttc ttgaacatct aaattaccat tataatccat tatatttcct
      601 tctattcctc taagaatctt tacgccttgc acttcacgtg gaagcaaatg caaattataa
      661 aaatagaatg gatgtggtcc tcctggcata ttaggtccat ggtctgatat tcctaagtat
      721 tttatattct tcttactcgc ctcttcgaca ttttccttga aagtactatg tgcatgtcca
      781 cttattagag tgtgacaatg tacatccata atagactttt tcattaaatt tcctcctaat
      841 attttacata aattcgtgaa aatagctaaa gggctatttc aaaactaaag ctgtcataat
      901 agacagtgta acgttttaaa acagccccat tttcaattat tttacacttc taaatcttta
      961 aatttatctt taaataattc tccaatactt cctaaagatt cctctacttc taggtattct
     1021 gttatatcct cttctggctc tctagttgct tcttttattg aaaccaacat tcttttagtc
     1081 tttggtttaa aatcaagaat cttaacttta acctcatctc caatattaac aacattagta
     1141 actttagcca ctctagtatc actcaattca gatattggta aatacgcatc tacaccttct
     1201 ttaatttgaa caaaagcccc tttttctatt attctaagaa ctttagcatt gacaacatca
     1261 tcaatttgta cttcttttgc tattaaatcc caagggtcat tatttatgtc ttttaatgcc
     1321 aaggcaattt ttttagtctc tttatttaca tccaacacat aaacttctac ttcttgtccc
     1381 tctgaaacaa catcttcaac actttcaact cttgtccaag ctaaattatt tatatgtgct
     1441 aatccttcaa tcccaccaat atccacaaat gcaccataag gcattatctt tgtcacctta
     1501 ccatctcttt tttgtccaac ttcaagagaa ttaaataagt cttctttagc tgctttgata
     1561 cgagcttttt cttcttctct ttcttgctta attcttgctc tttcagcttc tttttctgcc
     1621 ttaatttgag ctcttctttc ttctctctct ttatttatac gctcttgtaa tacatctcta
     1681 tgagatgcta caagtctatt ttttctaaca tccaattctt ttatataaac ttctaagtta
     1741 agaccaacaa atttagatgt atctgtaata aattttgtat caagttgaga aattggtata
     1801 aaaaatgtat atgtattgta attagcaaat acacctttat ctatattctt ttcaacattt
     1861 acagttatta ttctatgttc atcataagcc ttttgtaact tggcaaaatc atttcttttg
     1921 tcaagttgta atttagataa agttattgta ccatcttttt gagatacttt tgttattaca
     1981 gcagttattt catcacctat gtggtatata tcttctatat attgattttt ttctatgttc
     2041 aattcactta taggtattat tccatcaaac ccatagtcta atcctaattg tacttcatca
     2101 tttctcactg cagttatttt tccagttgtc aattctccaa ctttcacttt gctgaatact
     2161 tgttcttgct ggtcaagtaa ttcttgcatt gttaaatcat tttccattat aaacatcctt
     2221 tcctcttatt gattatcata tctgtaaata ttataccatt aatattgcaa atacttacat
     2281 ttttcttact ataattttta aaataaattc aacttcatat atttatacat tatagaaaat
     2341 atcttattaa attaaaatac aaattctaat ttttatctaa taaaatattt tcataaaaaa
     2401 tttatattgt atatcttatt cttttattgc tttaggtttt gatagaaaac atttataaaa
     2461 tataaagaaa gaaattgctg ttatagtagc agctgttata tctgcaactg gctctgctaa
     2521 aattactgca aaaagttttt cattaaagaa tgttgggagt ataaatatta aaggtattaa
     2581 aagtacaatc tttctaagta gagctaaaac caaagatatt gttgcttttc caagtgctaa
     2641 aaatgtctgt tgacaagcta tttgaatccc aaacataaaa gcaccagcaa aatatatttt
     2701 tatactccaa ctagtcatac taagtaactg cggattctta ttaaatatac taacaaaaaa
     2761 tgcaggaaat atcattaata aaagccataa aatagtagaa tatgtaaaac atgacaacaa
     2821 acacaactta aatgtctttt ttactctatc aagttgacct gaaccataat tataacttat
     2881 tattggttgt gctccttgtg taacccccat attaggaagc ataagaattt gcattatact
     2941 gctcattatg gccattgaac ctactgctaa atctcctcca tacatttgta atttactatt
     3001 taaggctata agtactaaac tttctgtaga ttgcattata aatggagaga ttcccaaacc
     3061 aagcactggt aagatgatac ttgcttttgg tatcatatac ttctttttta ttttaagtat
     3121 acttttcttt ccaaagagaa acataagtac ccaaatagca gaaacgcctt gtgcaattat
     3181 agtagctaat gcagctcctt ttacacccat attaaagcca aatataaaaa tcgggtcaag
     3241 tattatgttt attaaagcac ctatagcaac tgtcatcatt ccagttgtag caaacccttg
     3301 ggtatttata aaagtattca tacccattga aatctgaaca aacactgtac ctattaaata
     3361 aatatttaaa tagtccattg aatatccaat tgtagattca cttgctccaa acatccaaag
     3421 taaaggctct tttccaatct taaaacctat agtcaaaagt attgctaaaa tcaccagtac
     3481 agaaaaactg ttactcatta ttttctctgc ctcatcattg ttttgctcac ccatttttat
     3541 agcagctaat ggacctcctc ccataccgat taaataacta catgcagtta ttataattat
     3601 tattggaaat gccactccaa caccagccat agcaacttct ccattttcca ttctaccaat
     3661 aaaaatccta tcgactatgt tgtataaaac atttacaatt tgtgcgataa tagcaggtgt
     3721 agcaagttta aataataatt tacccacact ttcactacct aagttagtgt ttgaattttc
     3781 cattttaaaa cctcctcaat ccaataatta aaatgtatca ctaaaatttt attctatata
     3841 aaaatttcat tttattagca gacaaattta gagtactttc cctacaagta atactatact
     3901 atatttcttt tgccatagta aacattttag aactaactga agttaaatac tttatctcat
     3961 tttttattta ttattaaaaa tttttcacac atactcataa aaaaactatc caagtatatt
     4021 acttagatag ttttatatta tatgtaaaga aagagctgaa actataaccc ctgataacaa
     4081 attaccaaga aatactccca taaaagcatc ttttattctc atttttaata tggaagctat
     4141 agcagatgca gtccatgttc ctgtagttgg cagtggtata cctacaaata gtattatacc
     4201 aattatcgaa acacttttta atttcttcat tcttgagttt atttttctat caattacatc
     4261 agcaatccct ttaaatagtt tcttccctct taaaaattgt aatacatgtc tgaatgtcaa
     4321 tatcaaggga atcgatacca atgtagaacc tattacacta aatacataaa cccatatagg
     4381 atttaagccc attgcaatac ctataggaat ggctcccctc aattctgtta caggaaccat
     4441 agataaaatt aatatgtgta aatatcccaa actattccct cctattctaa gtgacattaa
     4501 gttatgaatt tatttgctct ataacctcac catccttttt actaactaaa aatgcatttt
     4561 tagtcaatga aagcattggt aaatcactct tagagtctga atatgcatat gaattttcat
     4621 aatcaatctc tatcccaagt ttacctaata cagcctttat tctttttaat ttttcaattc
     4681 ccttacaatt ttctccaatg aattgatttt tatatcttga attttcataa aaaaactccg
     4741 tgcctataac ctcgtctgca agaccataat acttaaaata tttcatatat gcatatggag
     4801 atgcagttat cataattata acattatctt cttctttatt tttgtaaata acctttttca
     4861 tagattcgtt aatttttttc attatgcaat catcaaaaaa cttttttaaa tcttcttcac
     4921 taaaatctac tattggataa aagattgcct cttttgcctt cttcaaaggt atcattctaa
     4981 ttaaatacat aaatagaata aaaataatgt atggcaactt tataatagtt ttaaaggttt
     5041 tattttttaa agagtaaata aaaaaatcta tcatgctatc agtatttata acagtataat
     5101 ctacatcaag taataataca ctttttttca taagtatttc cccatcctat cactaaaaat
     5161 atcactaaaa aatcaatatt attaaacatg taattatcca taagattacg tttattttta
     5221 aagcattatc ttttaggaca atatcagtag gattattttc atttgtagtg ttaattatat
     5281 aattatatct aagtacacca taaactacaa atattgttgt aaatatcata tgtggctttt
     5341 ctggattact tgtagaatac aatgcataac atacaattgt agaacttaat ataactatca
     5401 tcatttgatt tagattttct atgtcataat cttccaatat ttttctatgt tcaactgcat
     5461 catctcttag agtttctatt tcttttttac gttttccaag agctaagtac agagataaga
     5521 aaaatgtaca taaaattatc caatttgata aactaacact tatggctaca catcctgcat
     5581 aaaccctaag tataaaacca agagatattg aaaacacatc taatagtatt atattcttta
     5641 gtttaagaga atataatata ttgtttatca aatagagact gagaattata gaaagattaa
     5701 aactaacaac tgttgataaa cagactgaaa ttaaagctaa tactacaaat agtatattag
     5761 cacttttttt gcttattctt ccacttgcca ctggtctatt acatttttta ggatgttttt
     5821 tatctttatc catgtctata gcatcattca gtgcatatac tgatgatgac attaggcaaa
     5881 aacacacaaa cgcaataaac gtatttaaaa ataaatttat atttagtatg ttgtttgaaa
     5941 aaattatagc tgaaaataca aagaaattct ttatatattg ctttattctc attaacttta
     6001 tataatctga aattttagta agaagcctta tacttgtatt ggtctgtttc ataaacttac
     6061 taccctttca atattatttt ttaaacagga tatgatttta tccctatgtt taaaatttct
     6121 ataaatccat tgacattact gattactata gtaaccaata acagcgatag aaatatatta
     6181 tacatattct tttcattttt tctaagcgct ttcgtaaagt aagccaataa aattataact
     6241 gcaaaactaa aatttatagc cataatactt gaattgacta gattatatcc tactatacca
     6301 tgtaaagtta gattgaatat aattattccc atacatagct taggaactct tttttctgaa
     6361 ttttcaataa ctgataaaac acaaaaacat aaagctaata atgtaagaat tactggtatt
     6421 gtagcaactt ctccattttg caaaaataac ccttttttaa tgcttgctcc aggaaaataa
     6481 aaaaccgaat acaataatct ctctataatc attgcaatct tttccaaaat tggttctcca
     6541 cttataaatt tcgtatcata ctgtattaca tttccaaggt tcataaaact ctcataagac
     6601 ttcgttgaaa ctacacaaat atatgccatt aggataaatg taattaaatg ttttaacatt
     6661 tttcctattt ttaatttaaa ctcaaatatc atattaaaaa aatacaaata tatattacta
     6721 atagttattc caaatagagg aatagctgct aattctacta aataagaatt ttttcctttc
     6781 atcttatttg caataactat gaaaaatatt aatgaaaatt ggctatatat aaatctctct
     6841 acatttaaag tcataaatag ctgtggaaaa gaaaatatca ttatcatagt aagtaaaatt
     6901 aaagttaatc tctgcatttt tatcttttta atatgattaa agactaaagt tattgacata
     6961 gcactagtta ttatttgtag acaaattagt ccaaacccat aggcactatt aaaatctgtt
     7021 gtcccaggat ttgctattga agtacaaaat aggtatattg gatatgcaaa aattgatata
     7081 attgctgaaa acaatatgtg cttactattg tcttgactta tagcaaatac atttaaattg
     7141 aataaaactc ctgtatctgt atctaacaat acatcataag tattgtttaa atcataaaat
     7201 atagtagtct tagaaaaaaa tatcttagtt attattaagt aagttattac aactaatata
     7261 aacaaaaaca tctcgttttt atattttaat attttactca tcaatatatc accatccatt
     7321 cattatatag gatttgctga cacctacaat ttattcctta tatttaatag ccatcttcat
     7381 aatgttctta cttgttttat tgtaattgaa taactcttta tttacaacca caatatttac
     7441 t
//
LOCUS       DAELNY010000177         7380 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.180, whole genome shotgun sequence.
ACCESSION   DAELNY010000177 DAELNY010000000
VERSION     DAELNY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.180"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..978
                     /locus_tag="KPE55_003098"
     CDS             1..978
                     /locus_tag="KPE55_003098"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG0494140.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPNMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            971..1939
                     /locus_tag="KPE55_003099"
     CDS             971..1939
                     /locus_tag="KPE55_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG0494141.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2122..2712
                     /locus_tag="KPE55_003100"
     CDS             2122..2712
                     /locus_tag="KPE55_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0494142.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2731..3501
                     /locus_tag="KPE55_003101"
     CDS             2731..3501
                     /locus_tag="KPE55_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG0494143.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3638..4606
                     /gene="dusB"
                     /locus_tag="KPE55_003102"
     CDS             3638..4606
                     /gene="dusB"
                     /locus_tag="KPE55_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG0494144.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4715..5194
                     /gene="greA"
                     /locus_tag="KPE55_003103"
     CDS             4715..5194
                     /gene="greA"
                     /locus_tag="KPE55_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG0494145.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5209..6738
                     /gene="lysS"
                     /locus_tag="KPE55_003104"
     CDS             5209..6738
                     /gene="lysS"
                     /locus_tag="KPE55_003104"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG0494146.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7047..7241
                     /locus_tag="KPE55_003105"
     CDS             7047..7241
                     /locus_tag="KPE55_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494147.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2935 a          752 c         1499 g         2194 t
ORIGIN      
        1 atgagagata aaataataga gattatatta aataataaat ctgaatttat ttcaggagaa
       61 gaattatcaa aacaattagg ggtgtcacgt gcagctatat ggaaacatat gaaagcctta
      121 aaagaagagg gatataatat agaatctgta aataaaaaag ggtatcgatt agcggaaaat
      181 ccaactgatt tattaagccc acagaatatt tactataaat tagatacaga gtttattgga
      241 aaaaatattg aacatcttga cactatagat tcaacaaatg aacatgctaa aaggatagct
      301 ataaatagtg tagatgggac tgtgatttta agtgaacatc aagacatggg gaaaggacga
      361 ttgggaagaa gatgggattc aaaacctcat gaaggaatct ggatgagtat tatattaaaa
      421 ccaaatatgg aaccattcaa agctccattt ataactttaa tagcaggagc aagtgtaacg
      481 aaagctttaa acaatctagg agcaaataca ttaataaaat ggccaaatga tgttatttta
      541 aatggaaaaa aaatatgtgg tatattaaca gagttatctg ctgaaattga aagaacaaat
      601 tatgttgttc ttggaattgg aataaatgtt aaaataatgg attttgaaca agaaatagct
      661 gacaaagcca catcattgta taaagaaggg tatatgttat ccagagtaga tattgttagg
      721 aatgtattga ctgaatttga aaaattatat ttagattata ctgtaaataa ttccaaagaa
      781 aaaaccttaa aaatatgtag agattattct gcaattatag ataaaaatat atatgtatta
      841 aaaggagata atagagaact agtgaaatgt ttagacataa atgaagaggg aaatttatta
      901 gttcaaagaa gtgatggaag tataaaagaa attatttcag gtgaagtttc tataagagga
      961 gaaaaaggat atgtataata atattttgga tatagaaggc ttaaaggttg ggcaggttga
     1021 ggatagaaat ggtcttactg ggtgcacagt agtaatatgt gaagaaggag ctacttgcgg
     1081 tgttgatgta aggggctctg ctccagggac tagagaaacc gatttattag accctgtaaa
     1141 tatggttcaa aaagttcatg cagtagtttt ggctggaggt tctgcatatg gtcttgaatc
     1201 cacttgtggt gtaagtagat acttagaaga aaaaggaatt ggttttgatg taggtgtagg
     1261 taaagtgcct atagttgtag gagcagtttt atttgattta ggagtaggga gctttaaatg
     1321 tagaccagat ttaaagatgg gttatagagc ttgtgaagtt gcaaatactg atattttaaa
     1381 tcaaggaaat tatggagcag gttgtggagc cactgttgga aagataagag gtcaggaata
     1441 tgctatgaaa ggcggaatag gtagctattc tataaaatta gataatggtc tagttgtatc
     1501 tgcattaatt gctgtaaatg ctcttggaga tgtttatgaa aatggtaagg ttatagctgg
     1561 tgctttaaat gataaaaaga cagaaacaat tgatacatat aaattaatga aacaaggtga
     1621 aagtaaaggt ggatttagtg tagataatac tacaattggg ataattgcaa caaatgctaa
     1681 gttaaccaaa gctggatgta aaaagatatc tcaaatggga catgatggat atgctaagtc
     1741 tatttttcct atccatacac ctcatgatgg agatacaata ttcacaatat caacaggaga
     1801 aatagaagca gatataacat tagtaggttc attggctgta gaggttgtgg aaaaaagtat
     1861 aataaatgca gtaaaaaaag cggatagtgt agaaaatata atggcatata aagatatagt
     1921 aaaaaaacga gaaatatgat gaaattagca tatttttctt gtcaaattgt tgacaatggc
     1981 aactcatctt gataaaatta ttcttgttag agcgggcatc ctacaatggt agcttgactc
     2041 ataattataa gaaaaaaacg aaaaaattaa aaaattaagt agtccggcat cttaatgagc
     2101 tggaacggag gtgtattttt tatgatgaac acaagtgcat cagtaagaaa agtaaatgta
     2161 agaaagatga ctataatagg agtactatca gctatttcta ttatgctatc tatgactcca
     2221 ttgggattta ttccagttgg acctacaaaa gcaactataa tgcacatacc agttattatt
     2281 ggagctatta tggaaggacc aatagttggg gcagcaatag gatttatatt tgggatatca
     2341 agtctcttaa atgctataac aaacccaaca gtaacatctt ttgtttttat aaatccatta
     2401 gtttcaatat taccaagagt tatgataggt gtattagctt attatgtata tcagttaata
     2461 attaaggcta caaataaggt atacatatca ggtttaatta caggggcaat aggttctctg
     2521 ttaaatacag ctggagtttt aggaatgata tatgtcttat atgctgacaa gtatcttcat
     2581 gcaatgggta aaagtggttc agcaggaaaa gtaataatgg ctcttgccgc agcaaatggg
     2641 gtgccagaag caatagtagg ggctttagta gtagctgctg ttgccactgt acttaagaaa
     2701 agcaaaaaat agaaagaagg ttaatgacat atgcttctag tatttgatgt tggaaatact
     2761 aatatggttt taggtatata taaaggtgat aaattagtta attactggag aattaaaaca
     2821 gatagggaaa aaacgtctga tgaatatgga atcctgataa gtaacctatt tgattatgat
     2881 aatgtgaata taagtgatat tgatgatgtt ataatatcat ctgtagttcc gaatgttatg
     2941 cattctcttg aaaacttttg tataaagtac tgtaaaaaac agccattaat agtaggtcca
     3001 ggcataaaaa caggtctaaa tataaaatat gataatccaa aacaagttgg ggcagataga
     3061 atagttaatg ctgtagcagg gatagaaaag tatggagcac caagtatact tgttgatttt
     3121 ggaacagcaa ctacattttg tgctatctct gaaaaaggtg aatatttggg tggaacaata
     3181 gcaccaggaa taaaaatatc tagtgaggcg ttatttcaaa gtgcgtctaa attacctaga
     3241 gtagaattag ctaagccagg tatgactatt tgtaagagta ctgtatcagc catgcaatct
     3301 ggaataattt atggatatgt tggtttagtt gacaaaataa taagtatgat gaagaaagaa
     3361 ttgaattgtg atgatgttaa ggttatagct acaggtggat tagctaaact gattgcttca
     3421 gagacgaaaa gtatagatta tgtagatggt tttttaacac tagaaggatt gagaataata
     3481 tatgaaaaaa accaagaata attaatatac agggaattgg gttatatatc caattccctt
     3541 tttatgatag atttctagct atatgtggta tattataatg aggaaataag aatttaaagt
     3601 taaatcacaa aataaaggca aaaataggag aaaaacaatg aaaataggaa atttagaatt
     3661 gaaaaataaa gtttttctat cacctatggc aggtgtaaca gaccttccat ttagattaat
     3721 ttgtaaagaa cagggatgtg ggcttttata tacagaaatg ataaatggta aggcactttg
     3781 ctatgatgat gaaaatacta aaaaaatgct taagatagaa gaggaagaac atcctgtagc
     3841 agttcagata ttcggttcag aaccagagtt tatgggaaga gcagcagaga taatgaatga
     3901 ttattcaaat gagattctcg atataaatat ggggtgtcca gctccaaaag tagttaaaaa
     3961 tggagatggc tcagcactta tgaaaaatcc taagcttgca gaagaggtat tgagggcagt
     4021 agtgaagaac tcaaaaaagc cagtgacttt aaagattcga aaaggctggg atgataatag
     4081 tgtaaatgct gttgaaatag caaagatagc agaggattgt ggaataagtg ctttagcaat
     4141 acatggaaga actagagaac agttttatac aggcaaagca gactgggata taattgcaga
     4201 gattaagaaa aatttgagta tacctgttat aggaaatggt gatgtattta caatagaaga
     4261 ttcaataaat atgttagaca aaactggttg tgatgctata atgataggta ggggtgctca
     4321 aggtaaccct tggatattta aaagaataaa ccattatatg aatacaggcg agattttgcc
     4381 agagccaact cttaacgaaa aaataagtac tgccattaag catttaaaac ttgcagtaga
     4441 agaacatgga gagtatgtag ctgtaaggga aatgagaaaa cacatcgctt ggtatttaaa
     4501 aggacttaga aactctgcaa aattaaggga tgaaattaac aaaatagaag actatcaaga
     4561 agttgtgtcg aaattggagt actatatgga agactccttg acataaaaat aatagtgact
     4621 tataatatag cgcataaatt aaaaaaagat tataaaattt ttaaaatata attaggaaaa
     4681 ttcctaagaa atttgtgaag gagttgtatt tataatggaa gaaaataaag agtttttatt
     4741 gacacaagag ggatatgata aattagaaga agaattagaa aacttaaagg ttgtaaagag
     4801 aaaagaagtt gcagagagaa taaaagttgc tatatctttt ggtgatttat ctgaaaatgc
     4861 tgagtatgat gaagctaaaa aggaacaagc acaagttgaa gaaagaattt taaaattaga
     4921 aaatatggtt agaaaagctg ttatcataga tgaaagcaaa atagacctta atgttgttac
     4981 aataggatca atagttaaag ttaaggattt agaatttgat gaagaggttg aatatactat
     5041 agttggttcg acagaagcag acccatatga tggtaaaata tcaaacgaat ctcctgtagg
     5101 gaaagcactt ttaggtagag ctgcgaaaga agttgtggaa gtgcaagttc ctgatggagt
     5161 tgctaagttt gaaatattag aaataagaag ataatttgga ggaaaaaaat gaaaaataat
     5221 caacaaagca atgaagaagc gcaaattcaa gaagacctta gtgaagtact tcaagtaaga
     5281 agagataaac taaaaaaatt acaagagtca ggtagagatc cttttaaaga aagtagatat
     5341 gatagaactc attattctat ggatataaaa gataactttg attctcttga aggtaaaact
     5401 acaaaaatag caggacgtat aatgagcaag agaatacaag gaaaagctgg ctttatagat
     5461 attcaagacc aagagggaag aatccaatct tatgtaagac tagatgctat aggagaggaa
     5521 gaatatagtg tattttcaac atatgatatt ggagatatag ttggaattga aggagaaatc
     5581 tttaaaacta aaaaaggtga aatatctgta aaggctaaat ctgtagtatt actatgtaaa
     5641 tctttacaag ttttaccaga aaaatatcat ggattaaaag accaagaact aagatataga
     5701 caaagatatg ttgacttaat agtaaatcca gaagtaaaaa atgctttttt aattagaaca
     5761 aaagcattaa aagcattaag agcatactta gatgatagag gatttttaga agttgagact
     5821 ccaatattaa atactatagc aggtggggca aatgctagac catttataac taatcataat
     5881 actttacata taccaatgta tttaagaata gcgaatgaat tgtatttaaa aagacttatt
     5941 gttggtggat ttgataaagt ttatgaaatg ggaagaatgt ttagaaatga aggtatggat
     6001 ttaaaacata atcctgaata tactgcaata gaattatatc aagcatatgc agactatact
     6061 gatatgatgg aaataacaga aaatgttatt gcacatatgg ctgaagttgc tacaggaagt
     6121 atgatagtta actatcaagg aacagaaata aactttacac caccttggaa aagaatgagt
     6181 atggaagatt gtgttaaaga atattctggt gtagattttt ctactataaa tacagatgaa
     6241 gaggcactag aagttgctag agaaaaaggt atagagataa aacctggaat gagaagagga
     6301 gaagtaataa atgctttctt tgaagaattt ggagaagata aattaatcca accaacattt
     6361 ataactcacc atccagttga agtatctcca ttatctaaga gaaatgttga agacccaaga
     6421 agaactgata gatttgaggc atttgctaat aagtgggagt tagctaatgc attttctgaa
     6481 cttaatgacc ctattgacca aaaaggaaga tttatagacc aattgagaaa aagagaatta
     6541 ggtgatgatg aagcttttga aatggatgaa gatttcttaa aagcattaga ggtaggtcta
     6601 cctcctacag gaggactagg aataggtata gatagagtaa ttatgttatt aactaactct
     6661 ccatctataa gagatgtatt attattcccg actatgaaac ctattgataa caatccaaat
     6721 aaagaagaag aaaactaaga aatattagca ttctactaag ttttaaataa agtttagcta
     6781 ttaaattatc aataaatagc taaattttat ttaaaatgtc agtgtggaat gctttttata
     6841 tgcttcaaag attcttttag atatttaatt tataaattac caacaaacgt tagaaattga
     6901 tattagtttt tgttgattga gactttatat ttagaaaata tgattgaaag taatttatta
     6961 tgaaaatatt tagaataatt tatgagaagg taaaaagaat aatttatgag aatactatat
     7021 ataaaaaatt attgggggaa tgtctgatgt ttcaagtaat taatttttta tgtttatttt
     7081 gtgttggatt tttggttctt gatgttatga tatatttttg taaggatgca atggatattt
     7141 ttgagggtac aactaatgtt aataaagtta agagtataaa tgtttgggca gatagagtga
     7201 gagctaattc atattcttat gaaaataaaa ttgcaaaata ggtaaaatta tatatgcatg
     7261 tattgtttgt aatatcaagg gttatatcta ttagttttaa ttatatttat taaaaaaacc
     7321 aaaagttata acctttttta aaataaaaaa caaaaaaagt attgacgaaa catttttaag
//
LOCUS       DAELNY010000178         7300 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.181, whole genome shotgun sequence.
ACCESSION   DAELNY010000178 DAELNY010000000
VERSION     DAELNY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.181"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            115..1503
                     /locus_tag="KPE55_003106"
     CDS             115..1503
                     /locus_tag="KPE55_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494148.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            1531..2484
                     /locus_tag="KPE55_003107"
     CDS             1531..2484
                     /locus_tag="KPE55_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0494149.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            2544..2768
                     /locus_tag="KPE55_003108"
     CDS             2544..2768
                     /locus_tag="KPE55_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494150.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            2761..3273
                     /locus_tag="KPE55_003109"
     CDS             2761..3273
                     /locus_tag="KPE55_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG0494151.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            3305..4159
                     /locus_tag="KPE55_003110"
     CDS             3305..4159
                     /locus_tag="KPE55_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG0494152.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            4693..5487
                     /locus_tag="KPE55_003111"
     CDS             4693..5487
                     /locus_tag="KPE55_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0494153.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            5591..6415
                     /locus_tag="KPE55_003112"
     CDS             5591..6415
                     /locus_tag="KPE55_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0494154.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPDKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIVAIVLVAINPGNALYKRFSKNTTQIL"
     gene            7046..>7300
                     /locus_tag="KPE55_003113"
     CDS             7046..>7300
                     /locus_tag="KPE55_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0494155.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNT"
BASE COUNT         2818 a          817 c         1332 g         2333 t
ORIGIN      
        1 ttaataagaa taacttatat attccaatga aaacgatatt ggatttaagt aagtttgtct
       61 cataaaatga aagtatatca attgataata ctttaaaata agaagggaga aaaaatggaa
      121 gataaatttt atgcaaaagg caacggaaat aacggatata ttaaaaatct tgaagtttgt
      181 tcctttaata acttagatgg aacttgtgga atgtttcaaa tggctctgta caaaagagat
      241 gaaaaatact atttatatgg atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt
      301 agcgatatta cagaccctta taatccacaa tttataaaac attttcaaat gttagaccct
      361 aaagagtatc ctacaacaac aactcccaaa attcaaatag cagatgattt aatgatagta
      421 gcaatgagtt gtggaagtgg accaggagca cttgttgacc aagctaaatt agcaaatatt
      481 aagtgtgaag caggaattag aatatacagt ttaaaagaag accctttaaa tcctaagttt
      541 ttaggatatt gggattgtgg cttaaagcat gtaatgggtg ttcatagatt catgtacaat
      601 ggtggaagat atgtacattt atcaagtgat tgtgttggct ttgaaggtct gatttatagg
      661 gtcatagata taataaatcc tactaatcca gtggaaatag gtaaatggtg gagaccagac
      721 caatatgcag atggatatcc aaatagaact tttgatgcag gagcacctca ttgcccagaa
      781 tttatggata aaggatggct tcatggacct ccatttgtaa gagacggaaa agcatattgt
      841 ggttatggag gagctggttt agttgtatta gatgttgaag atttaacaag accaagatgc
      901 ttaggtgaat tgccatttac gcctgcattt tctagtagac ttgcaggtgc aagaactcat
      961 acagcattac cattgccagg aagagattta gtcgttgttc aaaatgaggg agaaagattc
     1021 cagttcttta aaccagataa cattacagat gttcaagcta tgaataatat acatatggtt
     1081 gatgttagtg acccaacaaa accaacatta attgctcaat ttccatatcc tgaagttcca
     1141 aaagatttcc cttatcctaa ctttaatgtt gcaggattag gaaaaccagg gccatttggt
     1201 ccacataatc ttcatgaacc aatggataat aagccatggt tagagcaaag aggagataga
     1261 gtatattgct gttatttcca tgcagggcta agggtttatg atgtatcaga cccatattat
     1321 atcaaagagc tagcatattt tataccacca aatccaaata aaacaccaga agaatcttat
     1381 ttcccaggat tcccaggacc acgcttggca gtaacagaag atcttatcgt tgatgataga
     1441 ggctacatca tcatagatgc tttagatgat ggattctata tattaaaaat gaaagaagat
     1501 taaataaata ctaaagaggg agagttgaaa atgaaaatac taggtatttc ttttggaaca
     1561 aaaaatggaa ataatgacac aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg
     1621 gctgaagtag aatttattca aatgtcatct attaacatta aacattgtac tggatgttgt
     1681 gcgtgtgtta aaactttatt atctgggaaa ggtagtatgt gtgtattaaa agatgatttt
     1741 gaatggttgc ttgacaaaat gaaagatgca gatggtatag tagtttcaga ccctattttt
     1801 gaagaagggg catcaggatt attccatact ataatggacc gttttggacc cagagcagat
     1861 agaggaaata acattatagg aacaaaagtt gcagaagtta taggaggaaa aattccagat
     1921 tcaagaatgc ttaatgataa agtaatatca tttatgggaa taggaggttc tgactggggt
     1981 acaagagttc aatgtgaaca tgctatgcta gctctcatac caatgtggaa agttatagat
     2041 aatgcttggt tcccatgggc aaaggaatta gtaatggatg atacaaggct tgttcaagtt
     2101 catcaaattg gtttaaatat agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa
     2161 ggagaagaag gtgtatgtcc acattgccac aatagattat tctaccttga gccaggtact
     2221 aaaaaggcaa tttgtgctct atgtggtata gttggagagt tagatactat aagtggaaaa
     2281 actatattta gttttccaga agagcaatta ggtcatgctc atgatacttt accaggtaag
     2341 tttatacatg gtgatgatat taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca
     2401 caagaattta aagaaagaaa aacatcttat aattttatag agccattaac taaagaaaag
     2461 caagctggag tttcattagg ataataatta aaatactaag actctttttt aagggatgtt
     2521 agatgttata ggaggcttat tgtatgaatg aaaagccaag ttttaaacaa attgaagtta
     2581 ttttagcagt tatttttttg gtacttacat attacaaggt tagattaact ccagcattta
     2641 ttgttatatt tataccacta tatttaagtt attttgcagt atctatgatt gtaaatcaaa
     2701 tagcaattaa gctattggag ataaggcaag ctagggaagc tactaattac aatataggaa
     2761 atggatagaa agaaaaaaga aatattaaca ctgtctatgg ggattgcttt actacctcct
     2821 ttatgggcag tattagcacc ttatatagga ataaaaactg gagctgtagc actaatctgt
     2881 gcaggattat atgtaactaa tggtaataaa caaaaagatg gattaaaaat aatgtttggt
     2941 ttttggtgtg gagatttatg ggcagtgtta gccattttaa ttatgggata tatgaatttc
     3001 aatcaaaacc tagaattatt tttaacactt agcatattag gtttttttgc agttgtaatt
     3061 gcatccttat ttgaaaaaat aattttccta ccatcatggc tttgtggttg ggctataggt
     3121 ctaactgtta tgacaggtga aaatattatt aatttacaag atatttgtat tcaaattgct
     3181 gtagctatgg cagtaggagt atggtacgtg ggtgcaggtg tagatttgtt tacaatatat
     3241 attttgagta aagataggaa aaagggaaat tgaaagcatg tgaatatact ttgattatag
     3301 gagaatggct atgggttttg aatttaaaat aatgagaagt ttaatatatg taggacttgc
     3361 caaggaagaa tatagaccta agctaatgga ctggttatat cgtcaccata ttccagatag
     3421 tattagcact tttggaccat attgtactaa atatgccttt tatcaagcat atcctacacc
     3481 aaatgaaggt gagcgttttg gtgcacgtaa gatgcaacta acagaacatt attggcttgt
     3541 agatgaacat atgcctgaga tggcaaatag aattatgaca gaatatatgc ctatggatgt
     3601 tctacgttgg caagggtgta taccagatgt agaaaataaa agggttcatg aaaatgtaga
     3661 aagtggagat gcaggacgtg cagtaggtgg agataatgga tgtccaccat ttatatttgc
     3721 ctttgttcca ataaactggg aagaagactt tagaggaaaa ggacgtactg tacaagatgg
     3781 accaaactat cgttggcaat ttatgattaa gtatccagat ggtatctcta aagaagaagg
     3841 agaaaaatgg ttctatgatg aggtagtgcc atactttaca aactgttgct atgttaatcg
     3901 ttttgtcagt agtaaaataa tgattaatta tggagcaact gcttttgacc gtgtatcaga
     3961 actatggttt gaaggggaag aagaatggta taaagctgtg gttgaagaga caaagtcgtt
     4021 tattaaaaaa ccagaatggg cacaagaaga ggagttccca tatttaaaac cacaattcaa
     4081 tatcgcatca gtattcttag gtgatatagc aactatggat gcatactcac agtatcgtgg
     4141 atatatacca atgagataaa aattatagtt tgtgataagt agaggaggca tttactctta
     4201 ctggtagaag aagacttatt gtatccttaa aagtaggtga atatatagtt ttatatttaa
     4261 atttatataa aactaagact aataaatatg aaacaataga attagaataa tttgataata
     4321 tttgttaaaa gagcatttag aatgtgttta aagattaaga tattcctttt atggtagcag
     4381 atagttgaaa taataaaact gttatcatga aaggaatatt tttatgtata ggaaattttt
     4441 aaaaataaaa gttaaatatg tttaagaaca tcttaaagtg aaagaatcat tgttagaaat
     4501 atgtagacaa tatagtcttt ataacgtatc atattaacaa gtttacactt ggattatgaa
     4561 ataaaaagag taattccaaa ggttgtaaat actatgctga ctttgatact tcaaagttta
     4621 ggtgattttg taattgttaa tagaaatacg tttaatgagc ataaagaatt tatctaataa
     4681 taggagaata taatgtatac aattggacaa gtatctaaat ttttaggaat atctagagat
     4741 acactaaaat tttatgagga caaaggatta gtaaatccta agaaaaatga tgaaaatgga
     4801 tatagaatat ataatcaggt tgatatctat gatattgcaa caataaactt ttatagagaa
     4861 attgatatag aaattaaaaa gattcaagaa ataagaaaaa gtaagagtat aaacaattta
     4921 gaactcttac tagaagagaa ggaacaaatc atactaaaag agatagaata taaaaagctt
     4981 ctcttaaaaa aggttcagtt agtcaaagaa gattgtgaca aaataaaaac atattttgga
     5041 aaatacacaa taaaagaaat gaagcctttg gaagtaaaag gtaaaataaa acatgttact
     5101 aactatgagg aatatgaagc cctaaaagag aatacaaaca acttaaaaaa agcagttaca
     5161 ttaactgatt taagaagagt aataaatttt aatgaagaag gtataataga agaaaaattt
     5221 ataattgtaa gggaagtaga tgactacaac aaaatcatag aaggtgaagt tctatctcat
     5281 cccaattgta tttatactat tattgaagat ggcagatggt caactggagg agaaaatatt
     5341 gataataaag tggaagacag attaagagaa atagcaatag aaaatgaatg tgaattactt
     5401 ggaatagtct atgttaatat attgcttact acttatgaag aagggattga gcgtatattt
     5461 ttagagattt atgcacctat aaaataatat aaaagcattg acctgggact tactcatagg
     5521 tttaaactca tgatataagc agttataact tatatagcaa ttaaatatta atttgaaatg
     5581 aggattactt atgagaacag agaaatcatt aaaaaggcct atagccagtt ttattattct
     5641 aacaaatata atttttttac cacttttttt attggtagta gtgactataa tgttaaaact
     5701 tccaacagtg atatctgaca ttgcgttgtg tatatcagcg tggtcatcca cttttgcatt
     5761 tatgatatta tttaaaaaaa tttatccaga taagaaattt ttagtacacg taaaagatag
     5821 atttagaaat aaacttaaat tttcaacagt atctataata attagtatac aagtcctaat
     5881 agctgtagta gttatattta tattagcatc taaaaatcat ggaataatgc ctaactttac
     5941 agtttcatca tggggagcat ttatatattt atttttaaaa aatttatttg caggatcatt
     6001 aggtgaggaa ttaggttgga gaggatttgt tcaaaatcat cttcagaaaa gacattctcc
     6061 tttaaaagca tccataatag taggtttttg gtgggggatg tggcatttac caatatggtt
     6121 tacaacagga ttcaaaggac ttgatttaat aaaatatatt atatttttta tgattgcaat
     6181 tatatcaatc agtattataa tgacaacttt ttataatcta aataaaactt tgataatacc
     6241 tattgtaata catcaactat ttaatttttt aatagggata attaatggag atttaataga
     6301 gcttataaat tactatgcaa tgttatattt aatagttgca attgtattgg tagctataaa
     6361 tccaggaaat gccttatata aaagattttc taaaaacaca acacaaatac tttaataata
     6421 taagtgagaa tcatatcaca aatgagagaa ataattctcc ttattaatta attttaatta
     6481 ataaatatta caatagattt atctggaatg gaaggtatgc agagtgtatt tcattagatg
     6541 aaacacttaa aagaatgaaa tagataagta tcaacttatt atatgagaga aactttaact
     6601 ttcttggttt aaaatattaa aattataagg aacatttatt attaaatact ttatttatat
     6661 gaattaattg aagaaaaagg atattaaact acaagttaaa aaataaattt aatatattag
     6721 aattataaat aaggcacatt aattttattt taaattaatg tgccttttat ttatggtaat
     6781 ttatgcataa ttagtatcgt tttgattctt taaataaatt tttcttggtt tcttcacata
     6841 aggaactttt tttaatttat aaaatctaca gttatattat agtatctatt ttagagaagt
     6901 ttaatttttg tatcaatcta aaacaaaagt tatacttaaa tgaaattgaa aacgataaaa
     6961 ttctcaaata aaataagtat tgtggaggtg atgaaagaat ccaatgataa tatgaagact
     7021 aatcttaata agaaagtagg ggttaatggt agtggacaaa gaaattgtat caattctcca
     7081 gattttatta aatagcacat tgattacaac taatggatta caagaggagt ctaattcatc
     7141 aaagagacaa attacttata gaataaataa aatcaatgac atgcttaaag taaaaaatgt
     7201 accacttata tgtttaagag ctgataaaga tattatcgtt agaaaagaaa caaaaaaagc
     7261 aattaaagaa attttagaaa aaaactattc taagaatact
//
LOCUS       DAELNY010000179         7278 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.182, whole genome shotgun sequence.
ACCESSION   DAELNY010000179 DAELNY010000000
VERSION     DAELNY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.182"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            594..1103
                     /locus_tag="KPE55_003114"
     CDS             594..1103
                     /locus_tag="KPE55_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG0494156.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1131..1325
                     /gene="rpmI"
                     /locus_tag="KPE55_003115"
     CDS             1131..1325
                     /gene="rpmI"
                     /locus_tag="KPE55_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG0494157.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1381..1737
                     /gene="rplT"
                     /locus_tag="KPE55_003116"
     CDS             1381..1737
                     /gene="rplT"
                     /locus_tag="KPE55_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG0494158.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2047..2592
                     /locus_tag="KPE55_003117"
     CDS             2047..2592
                     /locus_tag="KPE55_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494159.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            3027..4952
                     /locus_tag="KPE55_003118"
     CDS             3027..4952
                     /locus_tag="KPE55_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0494160.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5353..7257
                     /locus_tag="KPE55_003119"
     CDS             5353..7257
                     /locus_tag="KPE55_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0494161.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3059 a          718 c         1379 g         2122 t
ORIGIN      
        1 gtaacatatt agaatataat gtaggatttg ttatgtagta tagtagcata ttagaatata
       61 atgtaggact tgttatgtag tatagtaaca tattagaata taatgtagga tttgttatgt
      121 agtataatag tatattagag tacaatatag aatttgttat gtagtatagt agtatattag
      181 aatacaatat agaatttgtt gggtagtata gtgttatatt agaatacaat ataggatttg
      241 ttatgtagaa tatatagagt catgtagaat atacatatat aaaaggattt tgggtataaa
      301 tttaaaaatt aatagcaaaa tgagtagtat agacatataa ttagtagtag gaagtaatta
      361 aatttttcct aaacaaaaaa aatagttgca aataatttga ttaagtgata tagtaaataa
      421 ggtaagaata aaacaaagaa gaagtccgct tctcacctta cgccggaggt tagtaaggta
      481 aatataagtt aaagcttaat tttaatttaa gtttgtttaa atttggtaac ttatattgcg
      541 gatgattacg tcatccgctt tttattttat aaaaattttg gaggtgtcct acaattagca
      601 aagaattagc aatcaatgaa caaataaaag ataaggaaat aagagtcctt tcaccaacag
      661 gtgaacaact tggagtaatg cctactaaag aagctcaagc aatggctaat agcaaaaact
      721 tagatttggt tcaaatctcg ccgaatgcaa atccaccagt atgtaagata atggattatg
      781 gaaagtttag atatgagcaa gcgagaaaag agaaagaagc taagaaaaaa caaaaaacta
      841 tagttgtaaa agaagtaaga cttagaccag gtattgaaca aaatgattta aatacgaaag
      901 ctaataatgc tataaagttc cttaaaaaag gggataaagt caaagttgaa cttagattta
      961 gaggaagaga attaggacat aaagacatag gtaaagaagt tatgcttaag tttttagata
     1021 taataaaaga gtttggagaa ccaacaaaag cccctgcatt tgagggtaat aatatggttg
     1081 taattataga tccaaaaaaa tagatgatta actgagagga ggaatttgtc atgcctaaaa
     1141 tgaaaactca tagaggtgca gcaaagagat taaagaaaac aggaactgga aagttaaaga
     1201 gagctaaagc ttttaaaaaa catatattaa ctaaaaaatc tgcaaaaact aaaatgaact
     1261 taagaaaatc aactttagta agtgatggag atgctaagag aatagcacaa ttattaccat
     1321 actaagatac agattgtaaa taataactat agaagaggga tataaaagga ggtcataacg
     1381 atggcaagag ttaaaaaagc tatgaacgct cgtaaaaagc ataagaaaat attaaaactt
     1441 gcaaaaggat tcagaggatc tagaagcaaa ttatatagac cagcaaatac attcgtaatg
     1501 aaagcattaa aaaatgctta tataggaaga aagttaaaga agagagactt cagaaaactt
     1561 tggatacaaa gaataaatgc agctgctaga atgaatggaa tatcttattc aagattaatg
     1621 aatggattaa aattatctgg tgttgaagtt aacagaaaaa tgttatctga aatggctata
     1681 caagacccag aaggatttgc aaaattagca gaagttgcta aagcaaaatt agcttagtat
     1741 tgaatcatga gatgtaaagt ctttgagtta tataaataat tcaaggactt ttttattttt
     1801 tgaaaaatac ttattagtgt catgtacaac caattttata atgataaata aaataagcaa
     1861 tacaatagaa ttttatgaga tttattataa gaattgttac aattatttat gatagctata
     1921 aaaagtcata gtaaaaatga tattgatgta tgttgatata tgtataaagt ttccaatcaa
     1981 tcttgaatta atattatcaa catgataata ttatcttatt aataatataa ataaggggga
     2041 cataatatga atgaaaaact aatggattgt tttgtaaacc caattaaatg caagttattg
     2101 attgaaattt tttcaaataa tcgaactaca gctaaacaat taggagaaaa atttcaagat
     2161 atatcaaagg caacacttta tcgttacctt aataaaatgt tgaaagatga tattataaaa
     2221 gtggttgaag aaaataaaat aaggggaact gttgaaaaag tatatgctat gaagttagat
     2281 atagctgatg atgtgaacaa aattatagat gagaactctg gagaagaata tgctcaactg
     2341 tttactcaat atatgatggg atttttaaga gaattttatg aatacgctga aagaaaggaa
     2401 aaggatatcc taaaagatgg ttcaggattt tctgtctgtc cagtatatgc tagtattgat
     2461 gagatagttg aagtgagtaa aaaaattgct agtatactag aacctctaag aaataactca
     2521 catgaagaag gtagaagaat gcattctata ggagtgataa ttactccacc taaaaaggat
     2581 gaagggaaat aaatctgatt tacatggggt agagccaatt tatacaggga atcgttttat
     2641 gatttacata tgtatccaga gcaaagtatc tctgcttgga ttgtacttca ttgtaccatt
     2701 tcatccaatg acttggagtg gtacaatgaa gttttttaat ttttcatatt aatgctaaag
     2761 aaatgaactt tagaccacat atgcaattag gttgcattat acaattagac cacatataca
     2821 aaataaaaat cagtattttt atagcagata aattgactgt cattcttctt aaaaatcatc
     2881 tattagttat tgttagcata ttgaaagaaa ttatagtaat ctattgaata aatgaatatt
     2941 gcctataatc atataaactg ctatagttta aaataaagca ggacgttaaa ttttatagta
     3001 atctaaaaat ttaagagggg gattttatga aaataaaatt tgttattatg ccattggtga
     3061 tatttttaat ggtattattt gtggggtgtc aaagtacaaa agatacagaa gttaaagcaa
     3121 aagatacaga aattaaaata ttagggacag gtgatttaca ttcaattttg acagactcta
     3181 tggtttcgta tgtaaatgag gaaagggaaa agaataaaaa tctgcttatg gtagatgcgg
     3241 gagatttcta tggtacacaa agtagagaaa tgtgggaatg gtcttctgga aaaaagttaa
     3301 ttaatattaa aagaaatggt agagcagagt atgatgacat aattaagagt agtaaagatg
     3361 aagtacctat agttaaagat atggcaaagt tgaaatatga tgctgtagtt ttgggagata
     3421 atgaatttgt ttcaaatgat aagcaaagtc ttgataaact ggtttctgat tttaaaaata
     3481 ataatatgcc attggtatca gcaaatatat atgaacaatc tggagaaaat tatgtacaac
     3541 catatgtaat gaaaaatatt aagacagatg agggcaatgt aaaagttgga atactaggtc
     3601 ttactataaa agaagtaggg gaaacccttg gtcttgagga ttttgaaaag gataaaaaag
     3661 ctagagagct tggagaacaa gctgattaca aaggaaaatt atatgcaaat gatttagtag
     3721 aagatgcaaa aaaatggata aaagttatgg aaaaagaaag tccagacata atagttgcag
     3781 ttgtacactc aggggaagaa cctaaaacag atagaaatcc aggaaataga ataaaagagt
     3841 tagcaactac agtggatgga atagatgcaa ttgtagctgg tcatactcat gaaaaaatag
     3901 atgaacacaa atataaaaat aaatctggag atgaagtaat tgttacacaa ccaggttggc
     3961 atggagatag agtatcagag attaatttca aactaaataa agagaatgga aagtgggaca
     4021 taaaggaaaa gtctagtaaa ttaaaaataa ttgataaaga agtaagtata gttgctactg
     4081 cagatttaca ttcacatttc tctgataaat taacagttga ccttgttaat gaacgaagta
     4141 acccaattga acctgttgta gtagatgcag gcgacttctt agaccctcaa acagatgaaa
     4201 tgactcaatg gtataaagag tggaaaagta taagagataa taatagtgat aaaacgatta
     4261 gtagatgccc aatggtcatg gatatgaatc agggaatgta tgatgcagta gtacttggaa
     4321 atcatgaatt tgtatctgaa aaagatgaat tttggtcaga tgaacgcctt ttagatgctg
     4381 ttatagagga ttttgaacag gtggcaattt ctgtgttatc ggcaaatata tataggcaat
     4441 ctggtaaaaa ttatgttaaa ccatatatta taaaagatgt ggagacgagt gaaggtaatg
     4501 taaaagtagg aatattggga cttactataa aagaagtagg taaaggtctt aaaaatgtaa
     4561 acttacaaga gcaatttcaa tataaggata aattgtatgc aaatgattta gtggaagatg
     4621 caaagaaatg ggtaaaagtt atgaaaaaga aaaaaccaga tataatagtt gcagttgtac
     4681 attcaggaga agaaccaaaa aatccaaaac atccaggaaa tagaataaaa gaactagcaa
     4741 ctacagtaga tggtattgat gcaattgttg ctagtcatac tcatgaaaaa atagatgagc
     4801 atgaatataa aaataagtct ggagaaaaag ttatagtgac acaaccaggt gagcatggta
     4861 aatattattc taaaattacc tttaaagtaa ataaagaaaa gggttcgtgg actattaatg
     4921 ataaattcag taaaacaatt gaggttgaat aactttataa aatttataga taaagtaaat
     4981 tatctgatat tggcatgata tataagataa agtttctaat attagtataa tgcataatgt
     5041 gaaatatttt aataataaga gaataaacaa gacaaataaa aatattttaa catgtaagat
     5101 atagtatgaa ctattatact aatgcttact ttataatttg aacatgttag aataagtctt
     5161 atttgaaaat tttagtgtac tagagtaagt attattttac tagtaaaaaa tattgagaac
     5221 tatatgtaga tttgcttaaa aaggaattta catatagttc ttttcgtata ataaatagta
     5281 caatcttata tggtaaagat aaagtgtgat attaaatttt attatcatcc aaaatctaag
     5341 gaggagtctc ttatgaaaat aaaatttatt atagtagcat caataatatt tttaatagta
     5401 ttacttgtgg ggtgtcaaag tactaaagac acagaaatca atgtactcgc cacaagtgat
     5461 ttacattcaa ttattccaga taatttaatt tcctatgtag aagaggagag aaaatatgat
     5521 gagaatctac ttatggtaga tgcaggagat ttttttgaca tgcaaagtgg tgagatgaat
     5581 aagtggttta ctggtcaaaa attagtaaga tttaaagatg ggataccaga gtacaaaaaa
     5641 atagctgaac cacgtgaagg agaagttcca ttggctaaaa aaatggcaaa gctgaagtat
     5701 gatgcagtta catttgggaa tcatgaattt gtatctaatg acaagcagag ccttgataaa
     5761 ttggtttctg attttaaaaa taacaatata ccattagtat cagcaaacgt atatgaacaa
     5821 tctggagaaa actacgtaaa gccatatgta atgaaaaata taaatactga caagggaact
     5881 gtaaaggttg gtatacttgg tctgactata aaagaagtag gggaacgttc aaggtttaaa
     5941 agttttgaac aagataaaaa ggctagagaa cttggggaac aacctcaata caaagggaaa
     6001 ttatatgcaa atgatttagt agaagatgca aataagtggg taaatgttat ggaaaaaaaa
     6061 gaaaagccag atataatagt tgccattgta cattcaggtg aagagccaaa aaatcctaaa
     6121 aatccaggaa atagaataaa agaactagca acaactgtag agggaataga tgctattgta
     6181 gcaggtcata cacatgaaaa aatagaacag catacatata aaaataattc tggtgaagaa
     6241 gtaatagtaa cacagcctgg agctcatggt aatagtatat caaagattaa ttttaagttg
     6301 gataagaaga atgataagtg gtttataaaa gacaaatata gtgaattaaa ggtttttgga
     6361 aaggaagtaa atataattac aacttctggt ctgaatccaa aattttcaga tgaaatggca
     6421 gttaatttgt ttaatgaaag agatagagat gttcaaccta tctttataga tactggagat
     6481 tttttaaata ctaatacaaa agaaatgact aagtgggcta aagagtggca atacaatgaa
     6541 aataaaggca tctataaaaa aataaatgaa tggccaattg tatatcttgg ttatgatgct
     6601 gtagtacttg gtagccatga atttacagag gataaagaga cttttgagga aaaaaaatct
     6661 acattggatt atattataga aaactttgaa gaaatgaaga taccagtatt atcagctaat
     6721 atatatgatg aatctggaga aaattatgta aaaccttata taattcataa tgtagaaact
     6781 agggaaggaa atataaaggt tggagtatta ggtcttacta ctactgataa ggatgaagaa
     6841 gacttaagta actcaagctt aagcaaagac tcaaagactt cgaaaattcc tgaagaaaca
     6901 ggaaagttat atactaataa tctagtaaaa gatgcaagcg aatgggtaaa agttatgcaa
     6961 aaggaaagtc ctgatgtgat tgttgcagtt gtacattcag ataataatag tactctaaaa
     7021 aatccaagta gtgaagtcaa aaaacttgca atgtcagtag atggaataga tgctattgtt
     7081 gtaggtcata cgaaaaataa ggtagaagaa catatttaca aaaataagtc tggggatgag
     7141 gttatagtaa cacaatctgg agaagattgt tattcaaaaa taagtcttga actaagaaaa
     7201 gaaaatggca agtggaatat cataaacaaa tatagtaaag caataaaaat gaagtgaatt
     7261 tattgatata taaaccat
//
LOCUS       DAELNY010000180         7202 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.183, whole genome shotgun sequence.
ACCESSION   DAELNY010000180 DAELNY010000000
VERSION     DAELNY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.183"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(244..1083)
                     /locus_tag="KPE55_003120"
     CDS             complement(244..1083)
                     /locus_tag="KPE55_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0494162.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1107..1865)
                     /locus_tag="KPE55_003121"
     CDS             complement(1107..1865)
                     /locus_tag="KPE55_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0494163.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1912..2385)
                     /locus_tag="KPE55_003122"
     CDS             complement(1912..2385)
                     /locus_tag="KPE55_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0494164.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDIKCFIQIVPDAKKIDVGTLL"
     gene            complement(2414..2830)
                     /locus_tag="KPE55_003123"
     CDS             complement(2414..2830)
                     /locus_tag="KPE55_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0494165.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2877..3929)
                     /locus_tag="KPE55_003124"
     CDS             complement(2877..3929)
                     /locus_tag="KPE55_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0494166.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECNECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKGLGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4325..5005
                     /locus_tag="KPE55_003125"
     CDS             4325..5005
                     /locus_tag="KPE55_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0494167.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5138..5458)
                     /gene="sugE"
                     /locus_tag="KPE55_003126"
     CDS             complement(5138..5458)
                     /gene="sugE"
                     /locus_tag="KPE55_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG0494168.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5840..6667
                     /locus_tag="KPE55_003127"
     CDS             5840..6667
                     /locus_tag="KPE55_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0494169.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGDLIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2443 a         1297 c          846 g         2616 t
ORIGIN      
        1 aatttatatt tttttgaaat tacttaatat tttatataaa aatgaaaaag cacctcattc
       61 tgattaaaaa tctttctgag atacttttta aaatattaaa tactacatat agcttacata
      121 agtttaaaat ttaatctatt ataaaattac cctaattgcc aaacttttta atataaattt
      181 tccaacatcc tacaaatata atcaatagat atagaatcct aacttacacc aatatatatt
      241 tatttaaaat agtcctaaaa tacctgtaat agctcccact actatcagta taaacattat
      301 ttttattgga gaaagacctt tttttaaagc aaagagtact aataaagtaa gtaccaaagg
      361 aattaatcct ggaaatatct tatcaagtaa atctgtttga agactaaatt taaaatcacc
      421 actagtataa cttattggtg tagataaatt gataaaccta cctacaagcc cccccatgac
      481 taaggtacct agtatagaag ccccttctat aaccttattt actattccac cttgaagtat
      541 actttcaaca gcttttttcc catacttata tccatttgtc cacattccaa aagaaattga
      601 agtcattatt atatattgag ctattacaaa taatataggt cctgcaattg atgctcctcc
      661 ctcagttaaa ccaatacaaa cagccaacac tataggagtt ataactcctt gagttaaagt
      721 atcgccaatt cccgctagtg gacccatcaa gcctgttttg atagcattca taccatctcc
      781 acttatttca tctgctccat tagctttttc ttcttccata gctattgtta ttccatgtat
      841 aacacatcca catataggtt ctgtgttaaa aaatgctaga tgagtctcca cttcttgttt
      901 taactcttcc ttatctggat ataatttttt aagtactggc aacattgaat gtgaaaaagc
      961 tgttgattgc aatctttcat aattgtaatt agattgtgca aagaaaaacc atcttaacca
     1021 tgattttatt acatctttct tgcttaacgt tttcttagat acttcatttt ttatatctga
     1081 cataatattt tccccctcac atgtatttaa gatgtagcac tatcttttcc gtacttaatt
     1141 gaagtaattg taagagcaag tattactcca ataacagata ttactattat atctacttta
     1201 aaataaacag ccattagtat tcctaatatg aaaaagggca tagtaaactt gtttccaatc
     1261 aattttaaat tcatagcaat cccaagtgct ggcaacatag ccccaattac attaaataca
     1321 tgtaacacat tatcattcat tacttccaac ataccatcta tcgcatttgg accaaaataa
     1381 acaactaata atgctggtat aaaactcatt gcaaataaca gtatctgtgg tggtactata
     1441 tttataaatg ctactctatc aacattccct ttttgaacct ctctatcagc ccaatgtgca
     1501 aaaaaagaat ttactgtcat ctttccaatc caaagtacag ttgcaacagt tcccaatggt
     1561 acagctattg ccattgcagt acttacatct agcttagatg ctatagctaa agcagtacct
     1621 acatatcctg caaaagctgg gtcccccatc tgagctcccc ctgctgatat aaaacctaga
     1681 tatatcatat ttatacctgc tcctataatt gctccttgaa cagggtctcc taatataatt
     1741 cccgttacaa atccagccaa tagaggtcta ttccatgtaa aaaatccaac ataagtcaac
     1801 cacggactcc aagaaagata ataaaaaatt gctattaata ctgcttgaat tagtgaaatg
     1861 tgcatattct tcctccctat tttcttttaa agatttacaa gatagtcttc tttataataa
     1921 tgttcctaca tcaattttct ttgcatctgg tactatttgt ataaagcatt taatatcttt
     1981 tgatattagc tctctaaata tttctttttc ttcatctgac acagatatat ttttataaaa
     2041 actttttcta ccaagccctg ctcccatacc tccaacattc aattccttta aatctacccc
     2101 gctatttact aaacctaaga cagttttagg gtatttgacc aatactatta aattttcatc
     2161 tccattatat gattttaaga cttcagcacc tgattctaca tcatgtactt caaccttaac
     2221 agttgatgga gctgccattt gtaaaacttg aactataaat gggtccttta ctactaaatc
     2281 atccacgatt actatccttt ttgctgatgt aatcttacac caagcagtaa taacttgccc
     2341 atgtattaat ctatcatcaa ttcttgttaa tgctatatta ctcataatta ttcctccaga
     2401 tttttaattt attttacaac tttaaaacag ttttaacatc tttgattcct gatatcccaa
     2461 cttcctcaca taaattcttc aagtctttta acttcatagt atctctgttt aaaaataact
     2521 ctaaaagcat aggtaaattt acacctgtca aacattctaa ctcacatacc ttagcaaatt
     2581 tatttattat aatagcagat gtattaaatg gagttccgcc ataaaaatca agcaatacta
     2641 aaaccccttc atccttatat ttctcaattc catcttctaa ctttctacta aactcatcta
     2701 tgctgtcacc atggtttaat cctaaagttt ctacattttc ctgtttgcct actattaatt
     2761 cagcactttc taacattgct ttagccatat ttccatgtgt ggctataatt attcctgcca
     2821 tatgaatcat acctttctta acctttttta tatggattat tatttagtac tctaatctaa
     2881 ttatctatat taatcattat cttaccaaag aaaatatccc ttttgtatac tttttcaaaa
     2941 taagctggta cttcttctaa atttattcta tgtgttacta atttttcaac attcatatca
     3001 cccttttgca tatgatacaa tccagcactc cattcttttc ctgggaatgg aaaggagttt
     3061 ccaaaccaag ttccctttac agttaattca cttcttacta tcttttcaaa ttgctctcta
     3121 gtcaaagcta catccccata tggtacacct gcatacaata ctgtacctcc ctttttagct
     3181 agcaacagta cttgaccaca tgtcagtgga gtaccagccg attcaataac aatatctgca
     3241 ccatctccat cagttaacct ttttatttct tctacaatat tcttttcttt tgcatttata
     3301 caaatatctg cccctaaccc ttttgccaag tctaacttct catcaaatac atccacagct
     3361 attacttttg tagaaccaaa tatttttgcc cattgtattg aaaataatcc tattggacct
     3421 gtaccaagta ctacaacagt atctccaact ttagcttctg acctaaaaag accatgtcct
     3481 gctatacata ctggttctag cgcagcggca gtctcataac ttatctcatc tggtattttt
     3541 attaaatttc tctctttcac ctttgtatac tctgcaaaac aacctccaag ttctttattt
     3601 cctattattg ctacattatt acatcttgaa tacaatcctt ttttacattc attacattca
     3661 aaacatggca tggctggaca aacagcaact ctatctccta ttttaaaact ccttacttct
     3721 ttaccaacct gtgccacttc tccagaaaat tcatgcccta aaatttctcc aaccatatgt
     3781 ggacctgtct ttgaatattt tgatatatca gaaccacata ttccagctac ttttacttta
     3841 attattacat catctttttc taatattttt ggtacatcca catcctccac tcttgtgtct
     3901 cttataccat agaatctaac tgacttcact attaaccacc cctacttttt atttgagaat
     3961 atatccctat tgtttatatg ttaaatatct aaagcaactt tcatgccaat atatgtaagc
     4021 attgtattca tgcattttat aaaattatca ttaaatgtgt gtcaaagtgt gtcaaaagtt
     4081 tcttatatta aaacttatgt gtgtcaatat aaacgacact aacattttat attagttctc
     4141 ttattataag ttttataaaa tgtacttttt ttgttatttt tataaaattg ttcggaaatt
     4201 ccccttctat ttgtgatata atattaaata taaggtatca atataaattt tcatgcgaat
     4261 atcagaactt ttttgacact aatgccttat tcaatataat aatttaactt tttggaggtt
     4321 gtttatgaaa aaagtgtatc aaggaaagac taaagatgtt tttgaattag ataatggaaa
     4381 ttacttactt aaatttaaag acgatgtaac tggtaaagat ggagtatttg acccaggcga
     4441 aaatagtgta ggtctaagta tagatggaat tggtagagct aatttagaga catctgttaa
     4501 gttctttgaa attctaaaca atgctggaat taaaactcat tatgtaagtg ctaatttaga
     4561 agaagcaact atggaagttc taccagcaaa agtttttggt aatggattag aagttatttg
     4621 tcgttataga gctgttggta gtttcttacg tcgttatggt tcatgtgttg aagaaggtgc
     4681 taaactagat tgttacgtag aagcaacttt aaaagatgat gaccgttgtg accctcttat
     4741 aacatctgaa ggtttagaag ctttaaacat tatgactcaa gctcaatttg attcaatgaa
     4801 aagcatgact caaaaaattt ctaacatagt tcgtgatact atagctgaaa aaggtcttga
     4861 gctttatgat attaaatttg aatttggatt ctacaatgat gaagtaatct taatagatga
     4921 aatagcatct ggaaatatgc gtgtttataa agatggtgta atagttgacc ctatggattt
     4981 aactgctctt ttattagata aatagtatat acatataatt tacaaatata ataaacttat
     5041 aaataagata agctatgaag taaagacttt ttaaatgttg attattttta aacattttct
     5101 cgcctttaat ttcatagctt atttaaatta atatactcta attagtagat aatagcttta
     5161 accctacaat tcccaccaca ataaaaccta tacatgcaat tcttgatata ctaataggct
     5221 catttaaaat agccatgcct acaatagctg taccgacggt accaattcca gtccatattg
     5281 catatgaagt tccaagtgga agatgtttta gtgctaatga caaaaaataa aaacttacaa
     5341 tcattccaac aacagtaagt atacttggag ttaatttaga aaatccatgt gaatttttta
     5401 gttgaaaagc ccaaaacact tcaaacaaac ctgcaacaaa caaaaataac catttcataa
     5461 attcatccct cttttcaaat atgtctctta tttttccaat aaaataacct gagaaatatt
     5521 ataaaatatc tcccaggttt ttgtccttcc gtgttcacaa caaaaactat gttgtgggtt
     5581 ttatctcgga ccagaccaat cattaataat tgcggaaccc tataaaactc tattaattat
     5641 tctattttat ctataataat actatcaaca tatataaaaa aagtcaatat atgggttcca
     5701 tatataataa aaataacttt tatatacctt ttaatattct tattcacttc tctccatacc
     5761 catttgggtc aatctaagaa tatctaattt ttatttaata tgttataata acataaaaat
     5821 attgaaagga gtaatttcta tgaatttcta taaaattgca gtatgtcaga tgattactac
     5881 agaaaataaa attgaaaata tcaatcatgc tgtagatatg gtaacagaag ctgccatcaa
     5941 tggagctaaa attgtagttt tgccagaaat gtttaactgt ccgtatgaaa ataaatattt
     6001 tcccaaattt gctgaagaat atccaggtga aacaacaacg atattaagta aattagctga
     6061 gaaacatgga atttatctag tttctggtag tattcctgag ttagaagatg gaaaaatcta
     6121 taatacttgc tatgtatttg ataaaaatgg agatctaatt ggaaaacatc gtaaaatgca
     6181 cttatttgac attgaagtta caggaaaagt tagttttaaa gaatcagata ctcttacagc
     6241 tggaaatgat gtcactgtaa tagataccga atatggaaaa gttggtatag ctatttgcta
     6301 tgatattcgt ttccctgagt tatcacgttt aatggctctt aaaggtgctg aaatcgttat
     6361 cttacctgct gcatttaata tgactactgg tcccgcccat tgggagttat ctataagaat
     6421 gcgtgcttta gataatcaga tattctatgt tggtgctgca cctgctagaa atatgaatgc
     6481 ttcttatata gcttttggaa attcaagaat ttcagaccct tggggaagaa taattgctca
     6541 agctgatgaa aaagaatgta ttatttatgc tgacattgat agagatttaa ttcctgacat
     6601 aagacaacag ttaccacttc ttaagcacag aagaactgat ttatatgaac ttaatacttt
     6661 gaaataaaac agaacataac cccataaatc ataaattaag tgttaaaaaa atttatgggg
     6721 ttctcattaa tttgatttaa aattaatttc ataaaaataa tgctaaaatt aatttaatta
     6781 aataatacta ataattacta atgattatgc taattatcag catttactaa tttaatcact
     6841 attagttatt tcttttatta aatatcttta cttttttact tattatgtct tctagcttta
     6901 catttggatt atggtatttc tttctatttt tagtcacatc tttctattga attacttgct
     6961 taaaacacca atgcacacat gccagataat gttcacaatt atgtagaaat cttgctctac
     7021 cttcttcaat gttgatatta tctctcctag ttgatattat ctttcctaaa atttctttta
     7081 attaaaaaat aacattagtt gatatatcaa gtatatttta atcaactgaa aaaaacaata
     7141 ataattgata tatcaactaa ttaatgtaga ttaattcatt tttataattt atatactttt
     7201 ta
//
LOCUS       DAELNY010000181         6970 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.184, whole genome shotgun sequence.
ACCESSION   DAELNY010000181 DAELNY010000000
VERSION     DAELNY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.184"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      34..136
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            230..1606
                     /locus_tag="KPE55_003128"
     CDS             230..1606
                     /locus_tag="KPE55_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0494170.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1720..2865)
                     /locus_tag="KPE55_003129"
     CDS             complement(1720..2865)
                     /locus_tag="KPE55_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0494171.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVIVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3118..3825
                     /locus_tag="KPE55_003130"
     CDS             3118..3825
                     /locus_tag="KPE55_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494172.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3872..5467
                     /locus_tag="KPE55_003131"
     CDS             3872..5467
                     /locus_tag="KPE55_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494173.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIGSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            5570..6652
                     /locus_tag="KPE55_003132"
     CDS             5570..6652
                     /locus_tag="KPE55_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0494174.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            6801..6962
                     /locus_tag="KPE55_003133"
     CDS             6801..6962
                     /locus_tag="KPE55_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG0494175.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
BASE COUNT         2447 a          781 c         1079 g         2663 t
ORIGIN      
        1 ttatataaat taaaataata ttcgtaaaga aaaattcatg ttttgtactg tataacatat
       61 aattttgaca atatgggtca taagtttcta ccggaatacc gtaaatattc tgactatgta
      121 tactattatt tagttaatta aatatttaat taactgaatt atgaaggata cggctcagct
      181 tatcaactac tgtgtcgttt ttttgtaaaa tttaatagga ggtgttgtta tgcaaacaac
      241 aaacagtagt actatgcaga aactttttcc aattttgact aataaaaatg tcgacatgaa
      301 gaaggaaata attgcaggag taactacttt tttaactatg gcttatataa tagctgtaaa
      361 tccaaatatt ttatctgaga caggcatgcc tgcaggtgca cttgttacag gtacttgttt
      421 agcagcagca tttggatgta ttttaatggg tgtagttgct aaccttcctt ttgctttagc
      481 atcaggtatg ggtcttaatg cattttttgc atatacagta gttttacaaa tgggtgttcc
      541 ttgggaagta gctcttacag cagtatttgt agaaggtatt atttttatag tattatcagt
      601 aagtggagtt cgtgaggctg ttgtaaatgc gataccaaaa aatatgaaat tagctgttac
      661 aggtggtata ggaatattta ttgcacttat aggtctagtt aacagtggaa ttgttattgg
      721 agaccaagcc actcttatca aaatgggtag atttacacca gcagtaataa taacttgtgt
      781 aggattaata attattgctg tattagataa aaaaagagtt aaaggttcta tactttttgg
      841 aatagtagtg agttcattac ttgcttgggc atttgctttt ataaatccag agtatgcaca
      901 aaaattaggt atatatttac ctggtggatt atttaaattc gaaagtttag caccaatagc
      961 aggtaaaata gatttaggat atgtactaca tccaacaaat ataggtggtt ttttagtagt
     1021 agtttgtaca tttttatttg ttgatttttt tgatacagta ggtacattag ttggtgtttg
     1081 ttctaaagca aacatgttag atgaaaaagg gaatgttcct aatgtaggta gagccttact
     1141 tacagatgcg gttgcaacta cagttggtgc aggacttgga gtatctacag ttacaactta
     1201 tgttgagagt tcaacaggag ttatcgcagg tggaagaact ggatggacag caataacagt
     1261 tggaatttta ttcttagctg caatgttttt ctctcctgta tttatagcaa ttccatcatg
     1321 tgctacagca ccagctttaa tatatgttgg atatttaatg cttggtacag ttaaggatat
     1381 agagtttgat aatataactg aaggtgttcc agcttttgtt actattgctt gtatggcatt
     1441 aacatatagt ataggtgatg gtttaacttt aggtatatta acttatgttt tcgtaaatat
     1501 attttataat atatttggag ctaaaaaggc agaagacaaa aaacatgttt ctgtagtaat
     1561 gattgttctg gcaatattat ttataataaa actttggcta ctataatagg tattatttaa
     1621 aattttaatt taatataaaa taaagggctg taatttatta tcaattgtga tgataaacta
     1681 cagctctttt gtatttgttg tatttttgta tttattgtat tatttaataa tgtctattag
     1741 ttctccattt tgtaaagttt taggtgtaat tactatattg aactcttttt gcaaatcttt
     1801 tataatatct aattcttttt ttgttattat tgagattgtg ttaccacttc gatttcctct
     1861 agcagttcga ccacatctat gcaggtattc atttttactt tttggaaaat ccaagttaaa
     1921 aacatgactt acatccacaa tatctagtcc acgagcagat aaatcagttg taattaaaat
     1981 ctttgcttta ccaagtttaa atttatttat tgcattcttt ctatcttctt tttccatatt
     2041 tccaaaaatt cctattgctt tgtaattatg ataatttagt tttgatacta tgacttctat
     2101 atttttttca ttatttacaa atacaatagc tttttttgga tttgtagccg ctaatgcctt
     2161 tctaagaaaa gtaaatttat cccttatctc acctaataaa tatgagtgat tgatgttagg
     2221 atttatttgt gatttttcct ttgtttttat tatttcaaac tccttcataa gtttatcaca
     2281 aatttttata gtgctatcag taagacttgc agagcatcct ataatttgtc tatctcttaa
     2341 cgttgttctt attatgtctt ctatacaagt tatatttttt ccattcaata gattgtccac
     2401 ttcatctaag acaatagttt ttatattatg tgattttagc ttcttttgtt ttatcaaatc
     2461 taaaactctt ccacaagagc caattactat gtgtggtttt actgctttta tattttttat
     2521 ttgtttttgt atattgacct ccccaattaa agctaaagaa gtaacggaga gttctgcatt
     2581 tttagccaat aattcaacct ggtttgtaat ctgcattact aattcatgcg taggtgcaag
     2641 tataagagct tgtgtttctc tttttgatgt atcaattttt tcaaatatag gtaaaaggta
     2701 tgctagtgtt tttcctgttc cagtctgtga attaatcaat aaatccttat tttgaattat
     2761 attccctata acaagtgact gaacttctgt tggagaagtt atgtcttgtt ttttgagtcc
     2821 atctattaga gtggaactta tttttaattg ttcaaatgta ttcatatttt actccttata
     2881 ttttaataat tatagtcaac tattttttat tatacattgt ttattcaaat cgaactatat
     2941 attcttttat aattatgtgt aatttgaaag taaatatcaa ttatatttat acctttatat
     3001 ttctgatacc tttgcatttt atagatttct aaaataaaca aataaaagtt gtaattgttt
     3061 gatatttgtt atatattata tataacaaat atcaaaacta taatatggag ggaaattatg
     3121 ttaaaagtaa tcaatgtatc taaatcatat aaaaataaaa aagtagtaag tagtgtctca
     3181 tttgatgtaa atgatggaga gctttttggg tttataggac ataatggagc aggtaaaact
     3241 actactatta aagcaattgt tggaatacac gattttgaag agggagaggt actaataaat
     3301 tctaaatcta taaaaaaaca gcctgttgaa tgtaaaaagg aaatggcata tatacctgat
     3361 aatccagatt tatacgagtc attaactggt atacaatacc ttaattttat agctgacata
     3421 tttgaagttg ccaaagataa aagagaagag ttaataaaat attattcaaa taagtttgaa
     3481 ctcaataaag cattaggaga tttaatatct tcatattcac atggtatgaa acaaaaatta
     3541 gctataattt cggctttgat acacagccca aaaatcttaa ttcttgatga accttttgta
     3601 ggtcttgacc caaaagcttc ctttatattg aaggaaatta tgagggagtt ttgtgacaaa
     3661 ggtggttgta tatttttctc aacacacgtt ttagaagttg ctgaaaaaat ttgtgataag
     3721 attgccataa taaaagatgg aaatataatt gcttatggaa ctacagaaga agttaaggga
     3781 agtaattcac ttgaaagtat tttcatggaa ttaatagaga aataaaatgt tttttataca
     3841 atatagtgtc taaagtgagg aatgataaaa tatgagcaat ctaagtatat taataaaaaa
     3901 taatataata aatgaattta aattaaatgc gttaaaaaaa gctgatggta gagaaaaaca
     3961 aaaaataatg ggaatgatgc tttcagttgt tataatagcg ggtctattaa ttttttatgt
     4021 aacgagctta agtctctctt tagttgatat cttaaagcaa ataaatcaac tggaaatgct
     4081 actgatattt ggttttggat tatcaacatt aacaactatt gttacatcat tatataaaac
     4141 gtcatcatat ctatttcaag caaaagattt agaccttttg actagtttac ctataaaaga
     4201 atctactgta ctagcaagta agatattaat gttggttgga gcaaactatt tattttcttc
     4261 aatatgtatg attatacctg cagtggttta ctttttaaat tctgatgtaa gtataactta
     4321 tttcccttat ttgatattgc tatttttagc tttaccatta ttaccgatag ttgcttcatc
     4381 aataattttt ctatttatag ggcagatatc atcaagaata aaatacaaaa atttagtatt
     4441 aatcataggt agcattatat tgatgttgct ttatacccta cttatgtcaa gacttaattc
     4501 tatgtcagtt gaaatgctaa agaatagtgt gtctatttca gagacgatta gaaaagtata
     4561 tcctcctata tattattttg ttgatgctct taaaacagga agtatagttt catttttagc
     4621 atttatagcc atctcaataa taccattttt tatgtttgta actctgtttg caaaaaaatt
     4681 taaagatata aatgctaaga tgagtgaagg atataagtca aaaaattatg aatttaaggg
     4741 tcaaaaaact tcaagacctt caaaagcttt attaaaaaaa gaatttaaga gatatacttc
     4801 ttcgtttata tactttttaa attcatctgc aggtttgtta tttctaatag taggtacaat
     4861 tttaatatta ttatttggag cagataaagt gggtgagtta ctgaaattga atatagattt
     4921 taatcttata aaaatacaat tattgggagt attcttcttt ataatagtaa tgaattgtac
     4981 aacatactgt tcaatttctc ttgaaggaaa aaacttatgg atactgaaat cttctccaat
     5041 agatgaaaga gaaatattct ggtcaaaaat attgatgaat tttttattga atgcaccact
     5101 atctatttta tgtctaatat tagtatctat aagattagac tttgaaattc agtttattat
     5161 tgtatcaatc tgtttgattg gaagtttttc aatgtttgta gcattgatgg gattattatc
     5221 aaatttatta tacccaaacc ttgattggaa aaatgaggtg gctgtagtaa aaagaagtgc
     5281 aagtatgata gttacaatgt tagtatcatt agcttatgta attatacttg gaggtatata
     5341 tgtattactt ggaatttcgt ttttaaatat atatattatt ttggcaagta tacttacttt
     5401 gatattagat tttatgatat ggagaattat ttctactaaa ggagtaaaaa tatttaaaaa
     5461 tttataagtg tatttatgtc aaataaagac ttatcttttt tagtcttgca aataataggt
     5521 taagtagtat actatgtata gtgatatgaa aaaatttagg agaaaataaa tgagaaaatt
     5581 atccaaaaga gaaaaagatt ttattcaata tggattttta ttatttttga tatgtattac
     5641 tacttattta gtattaacta ctttagatat aaaactaata cctaaaatta taaggctagt
     5701 aaataagact tatatatttc ttggcatatt gacaattata gtatatatga taattgaatc
     5761 tattgtaact catttgataa taaatgcagt tcaaaaaaca aaagtaaaat ttattggtat
     5821 taaaatggct acaatgggat tttactataa cttagttact ccatttgcat ctgggagtca
     5881 accaatgcaa atatatgcac ttacaaaata tgatgtaagc cttagtaaat cagtggctat
     5941 agttactaat aaaactgtag tatttcaaag tacagttact gtattttgtg gaattttaat
     6001 tttgctaaat aagtcattac tgatggagca agtacattct gtgaaagtct tgataacttt
     6061 tggaatgatt atgaatgtaa gtatgttgtt aggtggacta ctaatagtat ttagtccaaa
     6121 acaagtaaaa atgatagcag aagttttgat taatttttta tctaaattta aaacatttaa
     6181 atttttagat tcaaaacgtg atagaataaa tcattatatt gatgattata attattctat
     6241 aaaaatattt attaaaaatg taagaatctt agttttaagt atattactta ctgtgttaca
     6301 attaatcgca tatttcagta tagtttattg tgtgtataaa gcctcaaatt taagaggagt
     6361 ttcatatgta catataatga cattacaggt ctttttatat atggctatat caccaattcc
     6421 aacaccagga aatgttggag ctaatgaggt tacatttttt actatgttct ccaatataat
     6481 tccaaaagaa ttattggggt actctgtact cttatatagt gggtttgtat actattttat
     6541 attaatagtt tcaggtttat ttacatttgg aacgcattat acattaaatt cttggaaaaa
     6601 ggaagataaa gtaaattcta atttagataa ggttattgaa aaattaaatt aataaaataa
     6661 ttagggaaat tctatatata aatatggtgt tgaataagct atattatatt gagatatttc
     6721 ccttttttga ttaaaattat agaaatttga aataataagt aataaacaga aaaagtgata
     6781 ttgttttgaa aggagaaact atgaaacaag ataaacaaaa tgaaaaacaa agtgagattt
     6841 ctaagataaa acaaagcaat gataataaac ttgaaatagc tcaagaatta catgtaagta
     6901 aaaatatagg gaatatgctt ggatatagaa tagataagaa aaaaatgaat agaccaagtt
     6961 aatgaaaatg
//
LOCUS       DAELNY010000182         6865 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.185, whole genome shotgun sequence.
ACCESSION   DAELNY010000182 DAELNY010000000
VERSION     DAELNY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.185"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            158..1075
                     /gene="pfkB"
                     /locus_tag="KPE55_003134"
     CDS             158..1075
                     /gene="pfkB"
                     /locus_tag="KPE55_003134"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0494176.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            1112..3031
                     /locus_tag="KPE55_003135"
     CDS             1112..3031
                     /locus_tag="KPE55_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0494177.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            3627..5153
                     /locus_tag="KPE55_003136"
     CDS             3627..5153
                     /locus_tag="KPE55_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0494178.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            5181..5738
                     /locus_tag="KPE55_003137"
     CDS             5181..5738
                     /locus_tag="KPE55_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494179.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            6260..>6865
                     /locus_tag="KPE55_003138"
     CDS             6260..>6865
                     /locus_tag="KPE55_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494180.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAIN"
BASE COUNT         2726 a          630 c         1280 g         2229 t
ORIGIN      
        1 attgtatata cacctgtaaa tttggataat ataaaactaa ataagataaa aacgataaat
       61 ttctctttta attaagagaa attttttgac tttcaaggat attaatataa agttaagata
      121 taaacataaa gcaaataaat aaaaaagagg tggcaatatg atttatacag tgacattaaa
      181 tccatcaata gattatatag ttaagctaga tgagctaaag acaggaagta caaatcgtgt
      241 aaaagaagaa tacgtttatc caggaggaaa aggtataaat gtatctcgca tacttaagga
      301 acttggaaat gataatacat ctttaggttt tataagtggt tttactggag aatatataat
      361 aagaacattg gaagaaaaag aattgaagac agattttata aagataaaaa gtggtttttc
      421 aagaataaat gtaaaaataa aggcgttaaa ggaaactgag attaatggac aaggtccaaa
      481 tatagatgat gaagacattg atactttata taaaaaactt gataagctaa atcaagatga
      541 tattttaata ttggcaggaa gtataccatc tactttagat gaaaaactat acgaaaatat
      601 aatggctaga ttagaaaaaa agaatataaa agttgtagta gatgcaacta aaaatcttct
      661 tcttaatgtt ttaaaatata aaccttttct aattaagcct aataacgacg aattagaaga
      721 actatttggt gttaaattaa attcaattga agacatggta aaatatgcta gaaggttaaa
      781 ggaaatggga gctataaatg tcttggtatc aatgggaaaa gatggagcat tgttgattac
      841 agaggaagat gaagtgctta taagtgatgt tccaaaagga aaagtaaaaa attctgtagg
      901 agcaggtgat tcaatggtag caggatttat atctgggtat ttgaatactg ggaaatatga
      961 ttatgctctt aaattgggag cagcttcagg tagtgcaact gctttttcat atgacttagc
     1021 caaaagagaa tacattgata aattggtaaa tgagatatct gtaaagcagt tttaaaatat
     1081 taaaaaatat atagaaaggt aattaatatt tatgaaaatt atagatttat tagatgaaaa
     1141 gtcaattaag ttaaatttaa attcaaaaac caaatcagaa gcaatagaag aattagtaga
     1201 tttggtcgca aatagtggaa atttaaatga taaagaaaac tacaaaaaag caattttggc
     1261 tcgtgaagaa atgagtacaa caggaattgg agaaggtgta gctatacctc atgctaaaaa
     1321 ttcttcagtt actaaagctt gtatagcagc agcagtgtca aaagaaggta tagattatga
     1381 atcgtttgac ggaagtttat caaatctatt ttttatgata gctgcaccag acggagctaa
     1441 caatactcat cttgaagtat tatcaagatt atctacaata cttatggatg aagactttag
     1501 aaataaactt ataaattcaa gctctgaaaa agaatttaag gaaataatag ataaaaagga
     1561 aagagaaaaa ttcagtgaag aatatcaaga tgagaaagtt actgaaaaaa atataataga
     1621 tactaaagag aatgatgcta ataagtatcc taaggtttta gcagtaacag catgtccaac
     1681 tggaatagca catacattta tggcagctga aagtttaaat aaaatggcag aaaacaaagg
     1741 tgtatcaata aaagtagaaa ctaatggttc agcaggtgtt aaaaataagc ttacaaaaga
     1801 tgaaatagaa aatgcaacat gtataatagt ggcggcagat aaaaatgttg agatggctag
     1861 atttaatggt aaaaaagtca taaaaactaa ggtggctgat ggtatacaca aagcggaaga
     1921 acttatagac aaggctgtaa atggagatgc tccaatttac catggaggag atggaagtca
     1981 caacgaatca aatgaagaaa gtgaaagtgg atttagaaaa gtatacaaac acttaatgaa
     2041 tggtgtatct aatatgttac catttgttat tggtggaggg atacttatag cgatagcctt
     2101 tttacttgat gattatacta ttaacccttc aaactttggt tcaaatacac ctatagcagc
     2161 attttttaaa ggaataggag ataaagcttt tggatttatg ttacctgtac ttgctggata
     2221 tatagcatac tctatatctg atagaccagc gtttgttgta ggatttgtag gaggtgctct
     2281 agcaggtgat ggtggttcag gttttttagg tgcactactt gcaggtttta tagcaggtta
     2341 tttagtagaa gggcttaaga aaatatttag tgtactacca gcttcattgg aaggaataaa
     2401 accagtattg ttataccctt tacttggaac acttttgatg ggaattataa tgacattttt
     2461 agtaatacct ccagttacag caattaataa tgcaatggtt ggatttttaa atggtttagg
     2521 tggaacttca aaaatagttt taggattagt attaggtgga atgatggctg tggatatggg
     2581 tggacctgta aataaagcag catatgtatt tggagttgca tcacttgaat cagggcagtt
     2641 tgaaataatg gcagcagtaa tggctggtgg tatggttccg ccacttgcta tagctcttgc
     2701 aactacattt tttaagaata gatttactaa agaagaaaga gattctggta aagttaatta
     2761 tgtaatggga ttatcatttg ttactgaagg agctatacct tttgcagctg gagacccact
     2821 tcatgttata ccagcttgtg ttggaggttc agcagtggca ggagcactta gtatgttgtt
     2881 caacgcagct cttcgtgcac ctcatggagg agtatttgtt ataccagtgg ttacccatcc
     2941 atttgcatat atattagcta tagctgtagg tgctttggtt ggaatgatgc ttttagcatt
     3001 acttaaaaaa ccattaaatc aagaagtata gcaattaaag gatattcaag aacttaaaat
     3061 atgatgtttt ataaaaatta atatattgct gaattatttt aaaataagga ttttatgagg
     3121 ctgttatata acgatttgta attgttatct tgacagtctt ttatttttat ataagtaaaa
     3181 taagtgtgtt ttgctttatt tgtatttaaa ttgtttttaa gttagaatat aaagaaaatt
     3241 atatattatt ttaatattca ctcgaactag aaaataatat ttgttgtatt tttttgtata
     3301 taaaatatat tgtaatatta aaaaaataat agtaaactga ttgcataaaa atgattttta
     3361 tataaaattt aagcagaaga ataaatttct aacgaaattg gatatttctt ctgtaaatac
     3421 atattcaaaa aaagaattat aagagtttag tcagcagtcg tgtaagtaat aaggacttta
     3481 taaatatata gaaatataat gatattaata taaccaagcg atttttaatt tagcttttta
     3541 ataagcttat tttttatact atttataata aataaactcc atgatgggaa aactattact
     3601 atttaatatg taaaggagta ttttaaatga atgaatcata ttggttttta aactcaagtc
     3661 caaaagaata taacaaatta ggtgaaaata taaaaacaga ttgtcttata gtaggtggtg
     3721 gaataacggg attgacaact gcatatttat tagcaaaaga aggtaaaaaa gtagtgcttg
     3781 ttgaggctga taaaatagga tatggaactt ctggaaggaa tactggtaag gtaacttgtc
     3841 agcatgatat attttactct aagatagaga aaaaatatgg attagataaa gcaaaatcat
     3901 attataatgc aaataatgaa gctttaaatt tagttgaaca aataatagaa gaaaacaata
     3961 taaaatgtga ttttaaaagg gaaacttctt ttgtatttac agaaaaagag gatactataa
     4021 aggatattaa agatgaatat cgtacatgta aaaaaatagg aataaattgt gagtatcatg
     4081 aaactataga aaatattcca ttagatataa agggggctat tagttttact aatcaaggtc
     4141 aatttaaccc aaagaagtat attgatggat tggcaaaagc agcagtaaat ttagggctaa
     4201 aaatatatga aaatacacca gtagtagacc ttgaaaaagg aaaaatatgt agggttaaaa
     4261 caagagagga taatataata gaagctgaga atgttataat ttcatctcat agtccatggt
     4321 atgatggatt gaatttttat tttgcaaaag aatatgcaga gcgtgcttat ttaatggttg
     4381 cagttttgga aaataaatta gcagatggta tgtttataag tatagatgac ccatcaataa
     4441 cttttagaca atataatgat ggaagtgaaa atttactgat ttttggagga ggagaccata
     4501 aagttggtca gggaggaact gagaaagaaa tatttgatga tttagagcat tatggtaaag
     4561 aagtctttaa ggtaaaagat tttaagggga aatggtctgc tcaagataat atgagttttg
     4621 acaatgttcc ttatattggt tatatcaaca aaagagaaga caatatttat gtagctacag
     4681 ggttctctaa atggggaatt actaatggaa ctgctgctgg aataataata aaagatttaa
     4741 taataaataa taattctgat tataaagata cttttaatcc atccagacta ggtagctact
     4801 tttcaaaaga ttttataaag gaaaatgcaa acgtagcaat aaattatgta agtggaaaat
     4861 taaaaatagg tagtggagat atgcctaaaa ataatggaga aggaaagatt gttaatattg
     4921 atggaaaaag atatggtgta tataaggatg ataatggaga tttttatatt gtagacacta
     4981 catgtactca tttaggatgt gaacttaatt ttaacagtga agaaaaaaca tgggactgtc
     5041 catgtcatgg ttctagattt gactataagg gaaatatact tgaaggacca gctcttaagc
     5101 cattaaaact ttatggacat ggtgataatg atgttaatcc taagttatta taaaattatt
     5161 tttaggaagg taaagtgtat atgaataaac ttttaaagcc aataactata ctattgtata
     5221 tagtttatat tttatctgcc gtattttttg tagcaaacaa agaatatgga agtctaggtc
     5281 tagtagcaat tagtttagtt ggtactttca ttttgagttt tataaataag caaactgaaa
     5341 gattattgag taaagaatta tatataatgc ttgctttatt tataatgttt tcatcattgt
     5401 taggtacttg ttttaatttt tatgatataa attattatga tgattttttg catttatggt
     5461 caggtataat atcttgtatt gtggcttttt ctataattaa atacttttat acacaaagag
     5521 agataagtaa aatgagtaaa gtgtttttag ttatattttt atttatgttt agtatgggag
     5581 tagcgagttt atgggaaata ggtgaatttt taatggatac ttttgttggg actactacac
     5641 aggccggagg gttagaagat actatgattg atatggtaga tgctttaata ggcacaatta
     5701 taactattcc ttttatagta aaaaaatcta atatatagtt ttaaattttt tatattaatt
     5761 ataatggatt taattaatca tgttgagttt caaggtctat atgattaatt aaatccattt
     5821 ttgttattta taattattat tgaatatatt tttacatata agatataagg tgatagatag
     5881 aaattgttgg attatgtaaa taattaatat ataataccat tatgggcaat aaatacaaat
     5941 caaatgataa tatagtctat ttatgtaaat gttatattgt atcctaagta taatagtaag
     6001 ttttttgata gtggtgtaga aagtaaaaat ctactagcct aaattgatgt atggattagt
     6061 ttaggaacag taattgcata tcttaagttt gatgtcatgt tgggctacta gaaataggtc
     6121 atctttttca cttgaatatc ttttctttaa acatggggag tatcatatca aaaacatatg
     6181 aaaattaagt gatttatgag aacagtaata ttttatatga tagtatagaa tagtaataaa
     6241 gtattaggag gaacattgag tgttaaaaaa gtggtttggt attgtgaaaa aaagacaaaa
     6301 aagtgagtca gtaaaagaag aaggtgaagt aatattaaaa aacgaaaaaa tattatctga
     6361 agaaaagttg atagatgaag aaggagttgt agttaatatt gataatcaaa tattaactaa
     6421 agtagtaaat gatgacaatg aaatagaaga aaaaataata gaagaagatt tgttaattag
     6481 tgaaaatacc ataaaattag atgataaaga agcaattatt aatgataaaa acatagaatt
     6541 atgtaaaaaa gaagttcaag ttgaaagtga aaaaataggt ttaaataagt ttgaaggact
     6601 ggaccaaaat gaaaattata atctagaaaa aaatgttatt gaagaaaaaa aagtaagtga
     6661 atgtttgaca gaagaagatt tagagtacat aaaagaaatt aaaataaaaa gaggaaaaag
     6721 tataaaagct ataaacttgt atactaaaga agagtgggtt tttgacactc atatacagtg
     6781 tagtaaaaaa ctcaaagttc cattagggta cataagagaa aatttaaaat atggatatat
     6841 ggattacttt ggagatgcaa taaat
//
LOCUS       DAELNY010000183         6849 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.186, whole genome shotgun sequence.
ACCESSION   DAELNY010000183 DAELNY010000000
VERSION     DAELNY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.186"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1265
                     /locus_tag="KPE55_003139"
     CDS             <1..1265
                     /locus_tag="KPE55_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0494181.1"
                     /translation="LFLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSI
                     VLNYEEKLFIESLILGMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNND
                     ILLYEQLICHLNVTMHQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITE
                     DEIGYLTLHFQTSLERKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETL
                     REEDLKRDNFNKTIDFIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTS
                     YKLMNRLMMSDYLIRELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNV
                     GKGILFPHGDMKYIKQSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFK
                     EISNLTEDENMLDILSKCDIEKVKDILI"
     gene            1406..1714
                     /locus_tag="KPE55_003140"
     CDS             1406..1714
                     /locus_tag="KPE55_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0494182.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            1737..2063
                     /locus_tag="KPE55_003141"
     CDS             1737..2063
                     /locus_tag="KPE55_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0494183.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            2102..3436
                     /gene="celB"
                     /locus_tag="KPE55_003142"
     CDS             2102..3436
                     /gene="celB"
                     /locus_tag="KPE55_003142"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG0494184.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            3447..3968
                     /locus_tag="KPE55_003143"
     CDS             3447..3968
                     /locus_tag="KPE55_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG0494185.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            4061..5020
                     /locus_tag="KPE55_003144"
     CDS             4061..5020
                     /locus_tag="KPE55_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG0494186.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIERRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            5069..6121
                     /locus_tag="KPE55_003145"
     CDS             5069..6121
                     /locus_tag="KPE55_003145"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG0494187.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            6124..6798
                     /locus_tag="KPE55_003146"
     CDS             6124..6798
                     /locus_tag="KPE55_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG0494188.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
BASE COUNT         2778 a          694 c         1289 g         2088 t
ORIGIN      
        1 tgctattttt gcttataaat ataatacgaa taaggctggg aaattcttta aaagtatcaa
       61 atactaagaa agagcttaag ctaaaaatgc ttatattgga tatagaagat gaattgaaat
      121 ctaatttatc catagtatta aattatgaag aaaaattgtt tatagaatct ttaatattgg
      181 gtatgaacaa acaaataaat agtaataata ttaagaaatc tttatatcta aatgaagaat
      241 tagcaagtta tacaaaaaaa attataaatt tagtatcaga agaatcagga ataaatttta
      301 ataatgatat attactttat gaacaattaa tatgtcattt gaatgttaca atgcatcaaa
      361 tgaaaagcaa tgtttactta gaaaatccac ttttagataa cataaaaact aaattttgtt
      421 ttttatttac agtaatatca agttcaatag aaattgaatt aaaaggaaaa caaataacgg
      481 aagatgaaat tggttatctt acacttcact ttcaaacctc tctagaaaga aaatacagca
      541 aaagggaatc aaataaaaag gcaagtattg tatgtccatt tagttttgga gtaagtatgc
      601 tattaaaagt aaagatagaa aagagatttg ataacataaa aattattgaa acattaagag
      661 aggaagattt aaaaagagat aactttaata agactataga ttttataatt gcatttcaag
      721 agtatgaaaa tatccaaaaa ccaattttta taacaacacc attatttact gatgaggatg
      781 agaataaact taaagagttc acaaacaaaa taaaagaaaa agacacttca tataaactta
      841 tgaacagatt aatgatgtct gattatttaa taagagagtt agaacatgat gatatttatg
      901 aagctataac atatttaact aatttattaa ttgaaaaaca ttatgcagaa aaagagtatt
      961 tacaatctat tataacaaga gaaaagtcat atccaaccaa tgttggaaaa ggaatattat
     1021 tccctcatgg agacatgaaa tatataaaac aatctgtttt atgttttgca aggttaaaaa
     1081 caccaataaa aattagaaat ggaaaggata taaagattat acttttgtta gcatataaaa
     1141 aagatgatga tagaaaagta tttagagaat tatttaaaga aatatctaat ttaacagagg
     1201 atgaaaatat gctagatatt ctaagtaaat gtgacattga aaaggtaaaa gatattctta
     1261 tataaaattc atagtgaact atggataagc acaaaatttt ccatgtgatg gaaaaagttg
     1321 tgcatgtttt tttgttttta taagatatta tatgtttgta aggtataaat aatctaaaag
     1381 tagagaagca acttaggagg aaaaaatgga agaaatagtt ttaaagatta taattcatgc
     1441 tggaaatgca aaatctatgc tttatgaggc acttgactat gcaaaggaaa acgattttaa
     1501 aaaagcagat gaattaattg aaaatgcaaa tgaagaaatt ttaaaagcac ataaagtaca
     1561 aactgaactt atacaaaagg aagctggggg agataaatca gatatatcta ttttattgat
     1621 tcactctcaa gaccatttaa tgacttgtat gagtgagaga aaccttataa aagaaatgat
     1681 tatgttaaga aaagaaattc aaaaactaaa ataaaaaatt aagggggatt taaaaaatga
     1741 aaagaaaagt atatttattt tgtagctttg gtatgtcaac aagtttactt gcagataaaa
     1801 tgcagaaggt agcagatgaa cacaatttac caatagaagt agaggcattt ccgatagcag
     1861 aaattgacaa aatagttgaa gaaaaaaatc cagattgtat attattagga ccacaagtta
     1921 aatacatgct aaaggagtta aaaccaaagt ttgaagctca aggaaaactt atagatataa
     1981 tcaatgaagt tgattatgga actatgaatg gggaaaaagt attaaaactt gctataaagc
     2041 taataaaatc taaacaagcg tagaattata gtaaaaaata ataggaaatg agggtggata
     2101 gatgaataga ttggaaagaa tacttatgcc attggctgaa aaaataggta aaaataaatt
     2161 attaattgca attagagatg gttttttagt atcatcacca ttattaataa taggttcatt
     2221 atttttatta gtagcaaatt ttcctataaa aggatggaat gaatttgtag gtcaaatttt
     2281 tggtccagat tgggctgtaa aattgcaaca accaactgta gcaacatttg aagtaatgac
     2341 tctacttgca gttttaggga ttggatattc ttatgcaaag caaatagatg tagacccaat
     2401 agcatcagca gcagttgcaa tagttgcctt ttttataata acaccatttg taataccata
     2461 tacaccagaa ggaacagaat cagtttacat ggtaacaggt ataccactag gttggatggg
     2521 ttctaaggga atgtttgtag gtatgataac agcaataacc tctgtaaaat tatttgctgc
     2581 agttgtaaaa aaaggatgga cattgaaaat gccagatgga gttcctccaa ctgtatcaaa
     2641 atcatttgca gcattaatac caagtgcaat agttatggta gttttctttt tagttaagat
     2701 agcttttgaa gctacaccat atggaagtat acatgaattt atatttaagt ttttacaaat
     2761 gccattatta aaattaggaa atagtttagg tgctatggca atcgcttata tattcttaca
     2821 tttattctgg ttctttggaa taaatggaag tagtgttgtt ggggctgtgt ataatccaat
     2881 attaaaaatt ctatcagctg aaaacttagc tgcttttcaa gctggttcaa aaataccaaa
     2941 cataataaca ggtcaattcc aagatatgtt tgcgacattt ggtggagcag gtagtacact
     3001 atcattagtc atagctatgt tattaatatg taaaagtaaa cgtataaagt caatgggaaa
     3061 actatcaatt ttaccaggaa tatttggtat aaatgagcca ttgatatttg gtctaccaat
     3121 aatgcttaac ccaatactat tgataccatt tgcaatagtt ccaacaataa atataataat
     3181 agcttacttc tgtatgtcag caggattagt gcctcttaca aatggagttc agttaccgtg
     3241 gacaactcca attatattct ctggtttttt aacaacagga tggcaaggtg cagtcttaca
     3301 attagtactt cttatactag gaatatttat gtatatacca tttataaaaa tgctagataa
     3361 gcaatacttg agagaagaaa atgaaactgt tgaagaggaa gatgatgata tatcttttga
     3421 tgatttagta ttataggagg aagattatga agaaaatagt tatgatattg gaccaaatac
     3481 aagctggagc tggaggaaaa gagaaaagta atatacctcc tgctggaaaa tcaagcccat
     3541 taggaccagg tgtaatgatg gagcaatttt taaatgaatc taaggtaata gctaccttat
     3601 tttgtgggga tgaatttttt gtaaataatc aagaagaagt aacgagtaag atgatagcta
     3661 tggtaaaaaa attaaaccct gatgtagtaa tctgtggacc aagctttaat tacgaaaact
     3721 tttctaagat gtcagcaata ctgtctaaaa atataaatga taaaacagat ataccagctt
     3781 ttgcagctat gtcagaagaa aatatagatg tgataaacga atataaaaat gacatatgta
     3841 tagttaagac tccaaaaaaa ggtggaattg gtcttaatga ttcattaaat aacatatgta
     3901 aattggcaaa agcaatagca aataaagaag atataacttt gatgaaagaa gagttttgtt
     3961 atcaataaga gtattaatta ttagcaagtt taaatattat actatttata ataagcatac
     4021 attagtaaaa tctatataat ttaaggaggg gaattttaac atgtggggaa tgatagcgac
     4081 atggagaatg gccctagaag gaataactga ggcatcagaa catttagaaa aaggcatgga
     4141 agcaggtgat gctgtagagc ttgctgtaag aagagttgaa gactatccat tctataaatc
     4201 ggttggatat ggtggacttc caaatgaaaa ttgtgaggta gaacttgacg ctgcatttat
     4261 ggatggtaac acattagata ttggcgcagt tgctggtata agagattatt ctaatcctgt
     4321 cagcatagct aggaagttaa gttatgagaa ggtaaataat tttttagttg gaataggtgc
     4381 tgaagattat gctcataaaa atggatttga aagaaaaaat atgttgactg atagagcgaa
     4441 gttacattat aaaaagagaa aaaaagagac tttagacaaa ggattaagcc catatgctgg
     4501 tcatgatact gttggtatga tatctttaga caaaactgga aaaatgtgtg cagctacatc
     4561 aacaagtgga ttgtttatga agaaaagagg tagaatagga gattctgcac tatctggttc
     4621 aggattctat gttgatagta gtataggagg agctacagct actggtttgg gagaagattt
     4681 aatgaaggga tgtatatctt atgagatagt tagacttatg aaagaaggga agcatccaca
     4741 agaagcatgt gatattgcag taaatgaact tgataaacag cttatcgaaa gaagaggaaa
     4801 agctggtgat ttatcattag ttgccatgaa taataaaggt gaatggggag tagctacaaa
     4861 tatagctgat tttacttttt cagtagtaac taaagaagaa tctcctactg tatatatttc
     4921 aaatagagta gatggaaaga ctatacataa ggttatatca aatgagtgga tggaagcata
     4981 tgaggctaga ataaaagcac caatagaagt aagtgagtaa agtagtggtt acataattaa
     5041 aatagagttt tataataagg agatttttat gaatatcata agtaataaaa tagatgagtt
     5101 aaaagaagat ttattaagtg atattataga tattgtaaaa atacctagtg tcaaagggga
     5161 gtcagaaaat ggcttccctt ttggcgaaaa agtaggggag gcacttaata aagcattaga
     5221 aatatcagaa aaattgggat ttaaggttag aaacttggat aattatattg gttatgcaga
     5281 gcatggagat agtgatgatt atgtttgtgt aattggacat gtagatgttg tacatgaagg
     5341 agatggttgg aagcatcagc cttataaggg agaagaaact aatggaagaa tttatggacg
     5401 aggagtacta gacaataaag gacctataat gtctgcctta tatggattat atgctattaa
     5461 agaattaaac ttaaaattag ataagagagt tagaataata tttggtacaa atgaagaaag
     5521 tggatttgaa gatataccat attatttaga aaaagaaaaa gcacctatta tgggatttac
     5581 accagattgt aaatatcctg ttgtatatgg tgaaaaaggc atggcaaaga ttagaataaa
     5641 aagtaaaatc aattatgaag aagatgtata cctaggtttt atagaaaata tgtctgaaaa
     5701 tgtgcttgtt acatataaag agctgaatat agaaaatagt gatactatat tagatataaa
     5761 ggtaaaatac gattttagtt ataaattgaa agatgtattg gatgaaataa aagctagttt
     5821 tccaaatagt atcgatatag aggtaatttc taattttaat cctgtatatt ttgataaaga
     5881 gagtaatctt gttaaaaagc ttcagctagc ttatgaaagg gttacatcat tagatggaac
     5941 tcccgtaaca actaatggag gtacgtatgc aaaagtaatg ccaaatatag ttccgtttgg
     6001 accttcattt ccaggacaaa aaggaatagc tcataatcct gatgaataca tggatataga
     6061 agatataata ttaaatgcaa agatatttgc aaatgcaata tatgaattag caaaggagta
     6121 gttatgttag aaataatagg aatgtcagta gaagacgcta aaattataga agattgtggt
     6181 gcagatagaa tagagttagt tagtgcttta actgaaggtg gattgacacc aagttttggt
     6241 cttatagaaa gtgttgttaa cagtgtaaag atacctgtga acgtaatgat aagacatcat
     6301 gcgaaaagtt ttgtatacag taaagaagat ataagtataa tgcaaaaaga tataggtgtg
     6361 gttaaagaga ttggtgctaa tggagttgta tttggtgtat tggataaaaa caataatata
     6421 gatgagaaaa atctaaatgt tttattgaaa tgttgtgata atcttgatgt tacatttcat
     6481 agagccatag atgaatcaaa tacaatagat agtgttaaaa ttttgaagga ttatgataaa
     6541 attacgaata tattaacatc aggagggaaa ggaagtatag ttgataacat acaaatgata
     6601 aagaatatga tgcttagttc taatcacata aaaattcttt taggtggagg tcttaatttt
     6661 aataatatag aaaagataaa ggaattgact aaggcaagta attttcattt tggtacagcg
     6721 attagaataa ataacagtcc ttttgaagat atagatagac aaaagcttaa acaactcgta
     6781 aatataattt caagatagta tatgaatata aaatacttag tttaaataaa gagagtaagt
     6841 taattataa
//
LOCUS       DAELNY010000184         6783 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.187, whole genome shotgun sequence.
ACCESSION   DAELNY010000184 DAELNY010000000
VERSION     DAELNY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.187"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..486
                     /locus_tag="KPE55_003147"
     CDS             <1..486
                     /locus_tag="KPE55_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0494189.1"
                     /translation="KLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKL
                     STKLIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVG
                     KFFASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQN
                     K"
     gene            918..1172
                     /locus_tag="KPE55_003148"
     CDS             918..1172
                     /locus_tag="KPE55_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494190.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1208..1531
                     /locus_tag="KPE55_003149"
     CDS             1208..1531
                     /locus_tag="KPE55_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494191.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1805..1957
                     /locus_tag="KPE55_003150"
     CDS             1805..1957
                     /locus_tag="KPE55_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494192.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2151..3074
                     /locus_tag="KPE55_003151"
     CDS             2151..3074
                     /locus_tag="KPE55_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG0494193.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3341..4420
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3341..3369
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4611..4763
                     /locus_tag="KPE55_003152"
     CDS             4611..4763
                     /locus_tag="KPE55_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494194.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4884..5697
                     /locus_tag="KPE55_003153"
                     /pseudo
     CDS             4884..5697
                     /locus_tag="KPE55_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5749..5910
                     /locus_tag="KPE55_003154"
     CDS             5749..5910
                     /locus_tag="KPE55_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494195.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(6239..6703)
                     /locus_tag="KPE55_003155"
     CDS             complement(6239..6703)
                     /locus_tag="KPE55_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494196.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
BASE COUNT         2769 a          658 c         1159 g         2197 t
ORIGIN      
        1 aaacttgcta aattacaaag agaattatca agaaaaacaa tcggtagttt aaatagaaat
       61 aaagcaagat taaaagttgc aagacttcaa gaacatatag ctaatcaaag aaatgatttt
      121 ctacaaaaac tatctactaa attaataaaa gagaacgata ttatttgcat agaagattta
      181 caagtaaaga atatgattag gaatcgtaaa ctatctcgct caatttctga tgtgtcatgg
      241 tcagagttta ttcgtcaatt agaatacaag gctaattggc atggtagaca aattgtaaag
      301 gtgggtaaat tctttgcaag ttcacaaata tgcaataaat gtgggtataa aaacgaggaa
      361 gtgaaggact taaatataag agaatggatt tatcctagtt gtaatgaaac tcacgataga
      421 gatataaatg caagtataaa tatattaaaa gaaggattaa gactaataac aattcaaaat
      481 aaataacgta tgtaagaacc gtaggaacta cggggatagc ctgtggagaa ttagtaagac
      541 atatgttagt atgcaaaatt ctgtgaagca ggaaacctgt gactttagtc atgggaggtt
      601 caggctagta ttactcatta ttgtattcaa aagctaaagt ggaaacctag tgaatatatg
      661 aatttagaag ttaatgagag agcattagca gccgactcaa tacttattaa aattgaagat
      721 gaagaagaaa caatgaaaga agctgaaaga gagaaaaaga gaggtagaag aaggtagaat
      781 ttaaagttaa aatagttaca ttgctaatat atgaaaatgt tacaaatttg gaaatagatg
      841 gatatatatg ttgtatagta taatgtagat aatatatcac caagtaaaaa acaacaaata
      901 aatctaaatt atgaggggtg gaaaataaag atattaaact tacaatatgt atatttgctt
      961 ttgtaattgg aataatagtg ttattttgct gtataggtct aggtcaattt aatatggatt
     1021 gtattataaa acaaaatggt gggtcaatag ctaccgaaca atacaacata tatttagaac
     1081 aatctatttc acaatataga aattttggtt ctatactggt tttactagga ggaataggaa
     1141 tactgtttga taaaagtgtc agaaaatact aaaaaagtat atttttattt aggatgtgat
     1201 gaatgatatg gcaagagatg tatttagtgt aaagatgaca ccactagaag cacagaaaat
     1261 ggtagaaaaa aatttggaag cagatttagt ttctgttgat atttataacc tagaaaataa
     1321 taagtctata atagttacaa tctttgaaaa atattatgca agaagcaata gtgatgcagg
     1381 tttggtagtg atttgtgaaa atactacagg aaaaacactt gttaaaatta cctcaactgg
     1441 tagtgcaatt ggactatttc agattgattg gggaacaggt aataatttga ttaaaagagt
     1501 aaaaaaaata cttattgatt atattattta attattttat gtagatattc ttaggttggt
     1561 atggaatttg tactaaccta ttaagtaatc aataaaacta aataaacata gaaagcactt
     1621 acaaatatgt aggtgctttt tattgaaaaa atagaaaata aaataaaaag taagatatag
     1681 gaaaaatatg taagaattat atgttataat aattgtagca agaagatgta atctacaatt
     1741 tatagagtgg agttcatgta ttaaaaaatt atcctcccaa cgataagaag ggaggtgaat
     1801 atacatggat aattttttat ttaatgtgtt agctagtgta acagctagtt acatagttta
     1861 tttgattagc aagttaatca aaaaagtaaa aagccactct accgcaaaga gtgacttaaa
     1921 gattgatata aattttaaat atcatcgtaa aaaataactt tcagttatag aacttcactc
     1981 taccgcaaat agattgtagt tcttcttgtt tttattatac cacaaattag aaaaaatatt
     2041 gcatataata ttttttatag tcaataaaaa tatgaaattt tttatacaca atgatgatga
     2101 atctaactag atgaataaat ttaattgtaa ataagaaagt acttacttaa atggtaggtg
     2161 cttttgtttt gccaaattat gctataatat tgagtgagat gagtaatttg tgggggaatt
     2221 ttataatgtg gatattagta ccaataataa caatagtatt acttataata gctgtttctt
     2281 ctatgcaata tattttagtt atgatagctt ttttgttgat tatatattct tttatagaaa
     2341 agaaaatagt tatgggtctt gtgtcagttc tttttttcac ttattcaatt tatctttgtg
     2401 caacttggga agataaatcc ttgatagcag ataataaagt tgagactgtt agggcacaaa
     2461 gagaaactgt agaaagagaa aaagaaatgg aaagaagaag aatacaagaa gaagtagaca
     2521 aagaaagata tattgaaaaa catggaatgg aaatatcaga aaatgattta aaagttaagc
     2581 tagaggcttt agtacctcaa gaatataagg ggaagaaata tgaattaaaa gtaggaaaat
     2641 ttaaaagata tagtatgtat tttgatttaa ctgtacaaaa tgaaaaattt agtaattctg
     2701 aagaatgtaa aaaatttgta aaagaaattg ctaatgatct taaaaaaatt aaaataagca
     2761 aggcgtactt taagtttcat tcaaaagatg acggcggtat atataattct gtttatattg
     2821 attattttag aaacatacaa aataatgtag ataatgttga aaatttagaa ttcaatgagt
     2881 ttgaacttaa aaccgaagaa gaagagaagc gagaacaaga gaaaatagaa caagaaaaaa
     2941 acagttataa taactacatt caaaacagag tggttgaccc actagataga ataaagaaac
     3001 taaaagagtt gctagattca ggagcaatta cacaggaaga atataataaa aagaaaaaag
     3061 aattattaga atagataata tagtaagcac ttacaagtat gtaggtgctt ttaaatttac
     3121 aaagtattcc attttaattt tatagtttag attttatgat ataataaaaa tatagaagtt
     3181 ttgcagtgtg cgatatttgt tacaaagtag ggcttaatac ttgaaatcta agatgttgag
     3241 ggtgcgtgat aagtgttatc aattgcacta ttgcccgctc actgcaattt taagagtatt
     3301 gtatatatgt aggtattgga aatgctaagt ttattttggg gttttagatt aactatatgg
     3361 aatgtaaata ttacatgggt aaaaattttg atgattttta tgacagtttt agattaacta
     3421 tatggaatgt aaattgtata gaattaagaa tttttttaat actaattata gttttagatt
     3481 aactatatgg aatgtaaata ttacatttaa tttgcattgc tttgatgact tctttgtttt
     3541 agattaacta tatggaatgt aaatattttt caatatcaat atagaacttt catctttccc
     3601 gttttagatt aactatatgg aatgtaaatc aagatatttt taataaaatt tctgagcgaa
     3661 ctgtttgttt tagattaact atatggaatg taaatgagga gattcagaca gcgactaaaa
     3721 agactgatat aaagttttag attaactata tggaatgtaa ataactttat atctccattt
     3781 gcatatccat ctgatgaacg ttttagatta actatatgga atgtaaatgc gtacaaggtt
     3841 gagaagctcc gcaatctcaa cgtcgtgttt tagattaact atatggaatg taaatatttg
     3901 aaaggaataa attttaaatg aatgaaagga taggttttag attaactata tggaatgtaa
     3961 atcagccaaa aacttcgaat ttgaactaaa atgcgtgggt tttagattaa ctatatggaa
     4021 tgtaaattct catgactatt gaataaatat aataaacaag catacgtttt agattaacta
     4081 tatggaatgt aaatttagaa tcaaaagcac ttaatcctag ttctttcata gttttagatt
     4141 aactatatgg aatgtaaata gctttttagc ttcgtctact actttataag caaagcgttt
     4201 tagattaact atatggaatg taaatgatga acaataaaac aatcatctaa agacgaagaa
     4261 agttttagat taactatatg gaatgtaaat gttcctgttg tgttttttac taatcccatt
     4321 ttatatgttt tagattaact atatggaatg taaattgctg tgatttcact aataaaacaa
     4381 ttaataattg tgttttagat taattatgtg gattcaaaat taaataaaca aagaaagcac
     4441 ttacaaatat gtaggtgctt ttattctgtt caaaattggt cggttgggta aaataattag
     4501 aaaaaattag taaaaacctc ttttctgtaa ctcgttacaa tattattatt aatgtaacga
     4561 gttacagaaa agaggtgaat aaaatagcaa ctaaaagtag ggcagagtat atgaaaaatc
     4621 gtcgaaaaga taaaagaggt tttagtgtac ttttagacaa agaaaagtta gataaatttg
     4681 atgaagtatt agaggaaaag aatctaacca agaaagaatg gctagaagaa aaaatcgacg
     4741 aggaactgga acaaaaggaa taaaaaataa gggtcactcc caccgaccaa agtttgagta
     4801 acccctatga cgtatactat catatatcaa ttatagtata tgtcattcct taaaaaaaat
     4861 caattattaa ggagtgtaat attatgaaaa atgaattaat gatgtttgaa ggaaaagaga
     4921 ttgaagtatt tgaatttgaa ggaagaattt tattcaactc aaaagatgta gcaaactgtt
     4981 tagacataaa aaatgttaat gagaatataa gacttatgaa taaaaagcaa gttgtaaagt
     5041 taagaaattc ggatatcagt aatactgaca tccgaaaact taataatgct ggagaaaatt
     5101 ttcttactga aagtggagtg tataaattaa tctttaaatc aagaaaagaa gaagctgaga
     5161 gattccaaga ctggataagt gatgaagttt taccaagcat aagacaaact ggtgcataca
     5221 taactaataa tgcaatcccg aaaagttgag agaaaaagca agtgagattg aaaaattaca
     5281 gttgcttata acagtacatc tatgttaaaa gaattgttag atgatgcagg ctttgacaat
     5341 aaatcaaaac tattaacagc taaaacatta tataagaaag caggaattga tttaccaata
     5401 gagataaacg aagaagaaca ttattttgat acaaaacaaa tagcatctaa actgaaaata
     5461 tattctaaga gtaataaacc agctcagatg gctgtttgtg agattattaa aaagattgat
     5521 ttagaagatg gtgaagttaa aggtgtttgg taaactaatg gttcttggac tggtactgta
     5581 aataaatata caaagagtgt aatagataag gtgagaactt ggatagagga aaataataga
     5641 cctaccaaga ttgcaggtga gaagaagaat tatcatgtat cttacaaaat tgagtaaatt
     5701 tatcagttgt attaaataat ttagtttagt tttgggggga ttaatacaat gtgtgagaat
     5761 ttacttgata atatgtatat tgaaaagaga aaagaagaat atagaattaa acttttgaaa
     5821 ataagagaaa cagatataga tatatataat aagttagaaa gtatagtata taaactttct
     5881 gagaaaaaat tagagaaaaa taataaatag acatataaag cacttggata ttctactgtt
     5941 tcaagtgctt tgtttggtat aaaatggtat aatggagata gagaattata ttaacccaaa
     6001 aatggagaaa atagagaaga tgatttagtt gacgctttat cagtaggagt acttgcaggt
     6061 aagacagaat ctctagtact attagttgga aatggaataa gtgatactca aaaaagttta
     6121 ataaataaaa gagaatttag aaatataact caaattggtg gaaatggaaa tgagaaagca
     6181 tttagtgaag tagaaaactt agttaaatag attagaaggt agcaactaga gttggttgtt
     6241 accttctttt tttatacttt ctctctctaa gtattttctt tttcatatct tctaatacat
     6301 ctaatgcagt agtataaaat tgtatattat ttctataagg tatagctatt ggaagaatgc
     6361 ccttgttaac taaaaaaata agaagtacaa taaataaaag tgtcataatc attctaataa
     6421 aataattagt gatatctgat aaatcaaatg ttaagttaat tgttgtagaa aatacaacag
     6481 ccataataga tatatacaaa gcaaaaatgg ggttactatt tttttgtaac tgtcctttaa
     6541 gcctagattg ctctatttct atatcttgaa tactttttat ggaatccatt tcactataat
     6601 acttataaac ttttaaataa tattttttat acttgtttaa agcttcttca ttatctttaa
     6661 ggtctttgtc acttaactct gatttaactt ccccaaaatc cataaaatct ctcctacaat
     6721 gttatatagt atactttaat ttatagcata tttaagaact tatatcagca atttgtcgaa
     6781 caa
//
LOCUS       DAELNY010000185         6759 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.188, whole genome shotgun sequence.
ACCESSION   DAELNY010000185 DAELNY010000000
VERSION     DAELNY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.188"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(60..1337)
                     /gene="hflX"
                     /locus_tag="KPE55_003156"
     CDS             complement(60..1337)
                     /gene="hflX"
                     /locus_tag="KPE55_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0494197.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVELASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRYKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            complement(2056..3888)
                     /locus_tag="KPE55_003157"
     CDS             complement(2056..3888)
                     /locus_tag="KPE55_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494198.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(3881..6127)
                     /locus_tag="KPE55_003158"
     CDS             complement(3881..6127)
                     /locus_tag="KPE55_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494199.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(6195..6656)
                     /locus_tag="KPE55_003159"
     CDS             complement(6195..6656)
                     /locus_tag="KPE55_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0494200.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
BASE COUNT         2153 a         1235 c          711 g         2660 t
ORIGIN      
        1 tcaatttata ataaattata aattaaatat attttaggct ataataagtt tttcaaaatt
       61 tactctaaag catacttttt atacttatta tattctatat tgctacattc tacatctaaa
      121 atagctccat cttctctgta ttctgtattt ataatgctcg tattttcgtt aaaataagaa
      181 actacatgac ctttatcata tggaattaag aacttatatc ttacataatc tttaaatatg
      241 ctatcacaaa tacattttat cattttatct atatttatat cttttttaac tgaaacaaat
      301 ataccttctt tatcgatact atctaaaatt ctatccaata cttctacatc tattaaatca
      361 attttattat acacatgtat cataggtata ccatctgcac ctatttgttt taaagtatct
      421 tctgtcactt taatatgact tttatagctt ggatttgaaa tatcaataac atgtaacagt
      481 acgtctgcct cacatgcttc ttctaatgtt gacctaaatg cttttactaa atcatgtggt
      541 agattactta caaaaccaac agtatcagat aataaaaatt ctttgttatt tgcaagaact
      601 atatttctga cagaagtttc aagtgtagca aataacatgt ttttctcaaa cactttcttt
      661 tcttcagagt ttttaaatat atctactagt ttattcatta ttgaagattt accagcattt
      721 gtatacccaa ctagagctac atttggaaga tttgattttc ttctcatact tctttgagtt
      781 tctctttgga attttaaatc atccaactcc ctatttaaag atgttatctt ttcttctatt
      841 cttctacggt caagttctaa ttttttctca ccagaacctc tatttttagt accaactcca
      901 ccactttgac gtcctaaatt ttcatttgaa cctataagtc ttggtaacat atattttaaa
      961 cttgctaatt ctacttgaag tttagcttct ctagtttttg ccctttgtgc aaatatatca
     1021 agtatcaatg ccgttctatc tataatttca caattaagct tttcttcaag attttttagt
     1081 tgtgatgtag aaagttcatt gtaaaatata actatctcta tattttcttt ctttattaag
     1141 tttaatattt cctcaacttt acctgtccca atgtaaaatg ctctattaac ctgctttgca
     1201 ttttgagtta tactccctac aacatcaata tcacatgctg aacataagtt ttcaagttca
     1261 atcattagtt cattaaagtc ctcagattta ttatttatgt ttaatcctac tgttattcct
     1321 tttttcgcca tatccatatt ctccttatct gctatttttt atttaatcta tagcacgaga
     1381 ttatggctct ataattaaat tcatcaagaa ttttgctttt tatcttatcc ttaaatgaca
     1441 cttcttattt actattataa taaccataat cgtgcattat tttctcaatc ttatataaaa
     1501 aatcactttc ttacacctcc tcccaaatat attatgcagt tttttcaata aaaaaactcc
     1561 ttacaaatct aagtaataca aaacaagcta cttatatcat aagaaggatt aaaattctaa
     1621 atatatatta aaaatacatt agtttaatta acttatatgt aattatcatt ttatatttaa
     1681 ccctattctt aaatttaagt agttatttgc atgattaata aaagtaataa aaacatatac
     1741 ttcaatataa tcatcaacta ttaaaattaa attctattta taataaaatt taaagaataa
     1801 aattaaatat acattttgat aatcacctcc aacattttcc ttactacata ttataataaa
     1861 ataaacactt tgtaaagaca taaaattatg tggctatctc aaaatataga gaatcctctt
     1921 tttcattgtt aaaatgataa aaatacactt tatttcatac acaaaagagt gccttatgaa
     1981 ttaaaaagtt cattttaagg caccctcatt tattttatct tattaattac atgtctatgc
     2041 taagatagtt ttttgttaca ttgcttcatc ttcttcaaat tgagaattgt aaagattggc
     2101 atagaatccg cctttcttta gaagttcctc atgatttcct tgttcaacta tatctccttc
     2161 attcattaca agaatcatat cagcatcacg tatagttgat aatctgtggg ctataacaaa
     2221 acttgttcta ccttccataa gattatccat agctttttga atcaatactt ctgttctagt
     2281 atcgacagaa cttgtagctt catctaatat taaaatttta ggatcagcta aaatagcacg
     2341 agctatagtc aataattgtt tttgaccttg agaaacatta cttgcttctt catttaattt
     2401 catcttatat ccatctggta atgtcttaat aaatctatga acatgtgcag atttagcagc
     2461 ctctataact tcttcatctg tagcattcaa cttaccatat cttatatttt ccataattga
     2521 accactaaat aaccatgtat cttgaagaac cataccaaac atttctctaa gctcactacg
     2581 attaaaatct ttaatattat gaccatcaat cagtattgaa ccgctattaa catcataaaa
     2641 tctcattaac agtttaacta ttgtagtttt acctgctcct gtaggtccaa caatagcaac
     2701 tttttgacca ggtttaacat ttactgagaa atcatttatt atagtcttgt ttgggttata
     2761 tccaaagttg acattttcaa aatcaatttt accttctaaa ccatctattg aaactgcatt
     2821 ttcaacaatt tggacttctt cttcctcttc taaaaactca aatactcttt cagaagctgc
     2881 tgctgttgat tgcaataaat tggcaacctg tgcaatctga gtcattggct gtgtaaagtt
     2941 tcttacatat tgtataaatg actgtatgtc accaacctca attgttttct taattgcaag
     3001 ccaacctcct aagattgata ccattacata tcccaagttt ccaataaaca tcataagtgg
     3061 ttgcattata cctgataaaa attgagattt ccacgctgaa ttatacaggc tatcatttag
     3121 tttatcaaac tcttcaatta cttcatcctc acgattaaat gcttttacta ttgtttggcc
     3181 actgtaagtt tcctcaactt gaccatttac atttcctaaa tattcttgtt gatatctaaa
     3241 gtatttttgt gatttcttta ttattcttga aattacaaac attgatattg gtaatattaa
     3301 aactgccact aatgtcataa ttccacttat agataacatc attatcaaaa cacctatcat
     3361 agttgttact gatgttataa gctgtgtcat actttgattt aaactttgat ttaaagtatc
     3421 tatatcattc gtaattctcg ataatatttc accatgcgtc ttagtatcaa aatacttcat
     3481 tggcaatcta tccaattttg ctgaaatctc ttttcttaaa ttataggata ctttttgaga
     3541 aattccactc attataaatc cttgtataaa agagaacagt gcacttatca aataaaggaa
     3601 aagtaaaaag gttaagattt ttccaattgc attaaaatct atacctacgt ttcctccaga
     3661 aactttactc actagccctt caaatatctt tgtagttgcc tttcctaaaa tttttggacc
     3721 tactatagaa aacgacgcac taccaatagc aaatacaatt actagaatta ttgatatttt
     3781 aaatttagat aaatatgcaa ttagttttcg catagtccct ttaaagtcat ttgctttttg
     3841 tcctgctccc atagattttc ccataggacc tcttcccatt ctactcatta gctagctcct
     3901 cctttgaaag ttgtgataaa gctatttgct cataaacttc gcaatttttt agaagctctt
     3961 tatgtgttcc tattccaact atttcgccct catttaaaac aattatctgt tctgcattta
     4021 agatagtact aattctttgt gcaactatta gaactgtact ttcagcagtt tttgatttta
     4081 aagccttacg aagagctgca tcagttttta agtcaagtgc tgaaaaacta tcatcaaata
     4141 tataaacttc tggatttttt gctattgctc ttgcaataga aagtctttgt ttttgacctc
     4201 cagatacatt tgttccacct tgagaaattt cagttttaaa agcttccggt ttagaatcta
     4261 taaattctgt agcttgagct atttcagctg ctgttctcat ttcttcatct gttgcgtctt
     4321 ttctaccata ttttaagttg gattctatag taccagaaaa caatactccc ttttgaggta
     4381 catatccaat tttttccctt aaatcatgtt gagaaacatt ttttatatta actccaccaa
     4441 gtaatatttc tccttcagta acatcaaaga aacgaggaat taaatttata agagttgact
     4501 taccactacc agtacttcct ataaaggcag ttgtttctcc tggctttgct gtaaaagata
     4561 tatttgttaa tataggttct tctgcgtctg gatacatgaa cgaaacattt ttaaattcaa
     4621 caagaccctt tttatcttcc ttaaactctt gtgcctctga tttttctggg tcttttatgc
     4681 ttatatctga cactaatact tcatctatac gtgtagctga aacggatgct cttggaagca
     4741 taatagatac cattgatatc attaagaatg acattattat ttgcattgta tattgtataa
     4801 atgccatcat atctccaact tgcattgctc cagaatcaac actgtatgca cctctccaaa
     4861 ctataagaac tgtgataagg ttcattataa gcatcatagc aggcatcata catgtcataa
     4921 ctctatttac aaaaagattt gttttagtta aatttttatt tgctttatca aatctttctt
     4981 cttcatgttt ttcagtacta aaagctctga taactggaat accagtaatt atttcacggc
     5041 ttactaagtt taacttatca acaagctttt gaaccagttt aaacttaggc ataaccactg
     5101 agaataacac tacaattaat gataagatag caactactgc tactgctata atccaggcca
     5161 ttgaagtatt tgtgtttaaa acctttatca ctccacctat agccattata ggtgcataga
     5221 atactattct aagcaacata accatcaaca tttgtatctg ttgaatatca tttgtactac
     5281 gagtaattaa tgaagctgta gaaaattcat tcatttcagt atttgaaaat gacataactt
     5341 ttctaaatac tccacttctt aaatttctac ctacacttgc tgctactctt gctgctaaaa
     5401 atcctactgt tattgtagct accatactaa tcattgctat accaagcatt ttagcaccag
     5461 taaataatat ataattattt tgagtttttt ctgtatcaat acctattttc tcatattctg
     5521 accttacata tgaaacagag ctttgtgtta tcatactctc tggcatactt tcaaagcctt
     5581 tgctcatctc tttagtcaat gtatctaact gttcctttgg cattgctgat aacaatttaa
     5641 atatatcaac atctccactt tgagtagctt gtggtggaag tttgctcatt aactgagctt
     5701 tcatagcttt tacctcttta ctatcgccct caagccctga tactattaaa atagctttac
     5761 caaaaataac atttaaatca tctatagtgt ctttgtcttt agtatttaac tcataaagag
     5821 attcatcttt tagacctggg tacttatctt tatacttaac ataatccttt ttattcagca
     5881 aagtataatt atctagtact ttatccttac tacttttatc cataaataaa gtaattttat
     5941 ttaattcact ctctctaatc acagatggaa cagagttttc tactccacct tgttgtatac
     6001 ctacatttac tatattggat gtgtattctg gtaaagaaag gtcacatacc gcttgaatta
     6061 ccaatagaca gactattatt actattgata atgccgattt ctttaaatat ttaattaatt
     6121 taaacataca tttcttccct tctgtaatat taattagtct aataacatta aattgaaaaa
     6181 cattatttta aactttaaaa atatttttca tcttcatgct tatgacatcc tgcttttaaa
     6241 ttatctcgta cttgcattag aagtcttcta agtactattt tttcttcttc actaaatcct
     6301 aaaaaaaact ctctttccaa ttccttgtta aattcttcaa ctttattaca tatttctaaa
     6361 cctttgtctg tcaaaaaaat cctagatact ctttggtcct tttcatcttg ttttctttcc
     6421 acaagtccag ctttttccat cctctttatc ataactgtag ttgtagatgc ttttactttc
     6481 cttttatctg ctaattcttt ttgactttgc ccatctttca tctttaaaga aaaaagtatt
     6541 tggggttgac catgatataa gcctaattta tcaaaataaa aatgagttct tataaaatat
     6601 attcgcatta attgcaagaa aacatcattt aatttttctt cagaattgct attcatactt
     6661 gccctcctta ttttttatat tacttagtca gctaagtatt attttactat actgcatgca
     6721 ttttttcaat atatttataa aaaattcata taaatttca
//
LOCUS       DAELNY010000186         6690 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.189, whole genome shotgun sequence.
ACCESSION   DAELNY010000186 DAELNY010000000
VERSION     DAELNY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.189"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..1135
                     /locus_tag="KPE55_003160"
     CDS             2..1135
                     /locus_tag="KPE55_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG0494201.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            2051..3073
                     /locus_tag="KPE55_003161"
     CDS             2051..3073
                     /locus_tag="KPE55_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0494202.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3369..4289
                     /gene="pyrB"
                     /locus_tag="KPE55_003162"
     CDS             3369..4289
                     /gene="pyrB"
                     /locus_tag="KPE55_003162"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG0494203.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4328..5020
                     /locus_tag="KPE55_003163"
     CDS             4328..5020
                     /locus_tag="KPE55_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG0494204.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            5013..5915
                     /locus_tag="KPE55_003164"
     CDS             5013..5915
                     /locus_tag="KPE55_003164"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0494205.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            6000..6584
                     /locus_tag="KPE55_003165"
     CDS             6000..6584
                     /locus_tag="KPE55_003165"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG0494206.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
BASE COUNT         2682 a          677 c         1213 g         2118 t
ORIGIN      
        1 tatgtctaac tttattctaa atgggatttt tagaacagtt atagtaagta gtctaagcat
       61 tcttctgata ttgattttca agaaaaatgt atttaaaaga tttagcaaaa aatttaatta
      121 ttatatatgg atgatagttg ttattaaatt atttttacca tttacatatt atacttttac
      181 aataaatatc ttgagaagta aaaaacacat taatattaat aacataaatt tagagggatt
      241 taataatgtt tcaactataa gtaataatat tatattgtac acttggatta taactgtagt
      301 agtgtattta atttacacta tatttaaata tataaaactt aaaaatttaa taaatgactt
      361 atcttatgat gttgatgata gagagattgt aaatctttat aaaaatatat tagaagaatt
      421 taatataaca aaagatataa aactcaaata ctcttatgaa gttgaaacac cagcattttt
      481 taattcatgt gtacttttgc caccacatga gtataaattg aaagagttag actggatttt
      541 tagacatgag cttatgcatt ttaagagtag ggacctttat ttaaaatatg taatcctgtt
      601 tttaaaaaca gtatattggt ttaatccatt tatatatata atggataaac atatagattt
      661 agattgtgaa ctttattgtg atgaaagagt tttaaaaaat cgaaattcag aggaaaaaaa
      721 ggattatgct ttaacaattc taaatgcaat gagaaaaggt tcaaatactt caaataaatt
      781 tatagctgga ctccataagc aatctgatat taaaaagaga gttatgaata tgtttaatga
      841 aaagtataag aatggtatat taatggcatt aactttatgt ttattatctt ctattacttt
      901 tttgaaggtt aattcaacta gtattgttaa tcttaatgta ttacctaaag gatttgaaga
      961 cttacgaact gttagcagta tacgtttttc agttatagga tttacatatg cagatgctcc
     1021 taaaaatatg agggaagaat ataaaataaa ttgtgaatca ttaggtttga tacctaaaga
     1081 ctctgataaa atagaagttg gtatagaata ttatgacagt ctattgaatg attaaataaa
     1141 tattatttta acagatatta attataaagt tttgaatagt aatttgttga gaaataaata
     1201 tatccttata aaaatagata cttatatacc aagaataaaa aatagtcaag atagtacaca
     1261 aggatatact tataaagaac ttaaataagt gaaataaatt attaattata aggtttataa
     1321 ttttaaaaaa ttagataaat tcaggtgtaa aatactataa aacacaattc ggaaaagtgt
     1381 atgttgaact tcatttaaaa tggaatttca aagttgctat agtaagtagt ttaagcattc
     1441 ttcttatatt aatctttgag aataatgtat ttaaaaaagt tagtaaaaaa tttaactatt
     1501 gtatattaat gatagttgct attgatatat tatctaaaaa aattaagtat gaaaagatta
     1561 ctgctaggac aatcttttca tacttaattt ttaatattta tattatccaa aattttcatc
     1621 tttttaactt agaatttgag aatcactatt aggaaatagg ctttgattta tgtagggtta
     1681 aaaatttcac agcatatcta actttatact aaatccatca aaaaatatac gccctataaa
     1741 agcgcataaa aaaacacata aaacaatatt tatgcgcact ttaacctcga atggtacaaa
     1801 attctttact tttacagacc catattatat aattaagtaa ataatgtata tttaggtgac
     1861 atattggata agctaattta tttattttat taatataatg atactataaa agttatataa
     1921 agtggtttac aaaaaagtta cagatagagc ttttattaat gtattgtgta ttagtatttt
     1981 atccttaggt acacttcacc tacaaaatag aatcataaga taattatggg aaaattttag
     2041 gaggtttatc gtgaagaaaa agatattaat accagtattt gcatcagtta tggcattatc
     2101 agtaagttca attgtaaatg cagatgaagt aaatgattca tctcaaaata aggatgataa
     2161 aacaaatact gaattaaatt taggtgaata taaagaagta aaatataaag ttgcaaaaat
     2221 aaaagatgga gtagctataa agataagaga agaaggtcaa gttcaaaaca tagcttattc
     2281 tggagatgaa tttacggttt taggaactca aggagaatgg gttaaggtta aagttgaaga
     2341 tggcgaaggt tggttagcta ctagatatgt agatatatct gaaggtgtag gatatactaa
     2401 cgctgataaa gtaaacttaa gaaaagataa gagtgaaagt tcagaagtaa ttgaagagct
     2461 ggaaaaagga agttctttat tggttttgga agataatgga gattggttaa aagttaaaga
     2521 tggggaaaca gaaggatatg taaaatcatc ttatatatct gataaagctc ctgtaataga
     2581 agagccacaa gtaaacccaa gtattgacca aaatgtagat tctacaaata ataatcaaca
     2641 aaacaatgca aatactaatc aacaaaataa taattcaaat gtgccaacag ctaactctaa
     2701 tgcagttcaa gctgtactta acttggcata ttctaagcaa ggttgtccat atgtttgggg
     2761 agcagaagga ccaaacactt ttgactgttc tggatttaca caatatgtgt atagaaatgc
     2821 agtaggaaag aatataccaa gaacatctaa agcacaatct aaatatggac aaactgtaag
     2881 taaagcaaat ttacaaccag gagacttagt attcttcaca actaatggtt ctggttctgt
     2941 tagtcatgtt ggaatatatg taggtggggg taatatgata cattcaccat caacaggaaa
     3001 aacagttagt gtaactagta taaattcatc atactataca gcaagatttg ttacagcaaa
     3061 aagaatatta taatcggttt gtaatatgac aaattgataa atacataaat atatataaac
     3121 gctctatatt atatttaatt taatcatgga aacaaaaaat gattatattt taaatgtact
     3181 atagagcgtt tttgtatcac tttaaatcta atcaagattc aagttaatat gactgttaaa
     3241 taggagcctt ttcatatgat gattatttgt gcaatccctt gacaggatag ggcaaaggtg
     3301 gtaagctact aacagtacaa aaatactttt aattttagtt cagagaggct aaaaaaggag
     3361 gacgaaagat gttaaaatca agaaatttaa tccaaccaga agacttttca atagaagaaa
     3421 tagatgaaat attagaatta gctcaaaaaa taattgacaa tccgtcaaaa tattctcgta
     3481 tatgtgaagg aaaactatta gctacattat tttatgaacc atcaacaaga acaagattaa
     3541 gctttgaatc tgctatgaac agattaggag gacgtgtagt aggcttttca gagccaaatt
     3601 catcctctgc atcaaaaggt gaaactctag gagatacaat gaggatagta tctggttatg
     3661 ttgatataat agctatgaga catcctcaat caggtgcagc ttcagaagca gctagatata
     3721 cggaagtgcc atttataaat gcaggagacg gcaagaacca gcatccaact caaacactta
     3781 cagacttact tactataaaa tcattaaaag gaactttaga aagccataca ataggattat
     3841 gtggtgattt aaaatatgga agaactgtgc attctttagt taaggctatg gcaagatata
     3901 aaaatacaaa gtttgtattt atagcgcctg aagagttaaa aatgccagat tatataaaag
     3961 aagctataaa gggacatgca tattatgaaa ctaataattt ggatgatgtt ataggaagct
     4021 tggatgtttt atatatgact agagttcaac aagaaagatt tgaagataaa tcagagtatg
     4081 aaagacttaa aaactattat attttaaata aagctaaatt agaaaaggca tctaaagata
     4141 tgttagtaat gcatccatta ccaagagtaa atgaaataga catagatgta gatagtgatg
     4201 atagagctgt atattttaaa caagctaaat atggaatgta tgttagaatg gctttaataa
     4261 taaaactttt aggtataaat gaagactaat agttatcata taatagtaaa acaaaggaga
     4321 ttttgggatg tataaaattc tggaaaatat atatataggc gaagatatgt atagaatgaa
     4381 agtaaaaggt aattttgaag gtaaaatggg acaattctat atgttaagag catgggatac
     4441 ttatccagta ctttcaagac ctataagtat acatgatata gatgaagaag ggataacttt
     4501 tctttataaa gtagtaggag aaggaactca aattttgagt aacctaaagg taaatgataa
     4561 tataaaattg gaaggccctt atggtaatgg atatgctaag gttgatggaa aggtagctct
     4621 agtaggagga ggaatcggtg tagctccact atatttggtt gctaaaaata taaaaaattg
     4681 tgatgcatat cttgggttta gagaagatgt tatattagaa gatgaatata aacaagtatg
     4741 taataaagtc tatacaacag taggaaatac ttttgtaaca gatataattg atgttgagaa
     4801 atatgattat atattaacat gtggtccaac tccaatgatg gaaaaattag ttaagatggt
     4861 agaaggtact aaaacgagaa ttatggtatc tcttgaaaac catatggctt gtggagtagg
     4921 tgcctgttta gtatgtacgt gtaaaactaa tggaggaaat aaaaagactt gtaaagatgg
     4981 tccagtgttc tggggagagg acgtgatttt taatgggtaa tttaagtgtg aaatttggaa
     5041 gtgtagaatt taaaaatcca gtaataatgg cgtctggaac ttttggattt ggaaaagaat
     5101 ataatgagat ttatgatata caaaaacttg gtggtataag tagcaagggt cttactttaa
     5161 ataaaaaacc tggtaacaat ggtatgagag ttcatgagac accatccggt atgatgaata
     5221 gtgttgggct tgaaaaccct ggagttcaag gatttataga ctatgaatta ccttttttta
     5281 gtaaactaga cttagtcaga atagctaatg ttggaggtgg gacattagaa gactacttat
     5341 taggggttca aatgttaaat gacaagccta tagacattat tgaattaaat atatcttgcc
     5401 caaatgtcaa agctggtgga atggcttttg gtataaaaaa tgaagtggca agagaagttg
     5461 ttcgtgaggt aagaaacata acgaaactgc ctttagtaat aaaattatca cccaatgcag
     5521 aagatattgt aggaatggca aaagtttgtg aagaagaagg tgctgatgga gtttcattag
     5581 ttaatacttt taaagcaatg gcaattgata taaaaaatag aagaccagta tttgaaaatg
     5641 tatatgctgg gttatctgga ccagctataa aacctatagc tttgagaatg gtacatgaag
     5701 tgtgtaaaaa tgtaaatata cctgtaatgg gaatgggcgg aataactaaa gccacagatg
     5761 ctatagagtt tataatggca ggagctactt gtatccaagt tggaactgca aattttatga
     5821 atcctagaat aggtattgaa ataatagatg gaattaatga atttatggat agagaaggaa
     5881 taaaaagttt agacgaaata agaggaatta tataaggtag aataaatagt attttaaaga
     5941 taaaaaatat agattaaata aacaataatt ttataacatt aacatggagg aaaaataata
     6001 tgagtaaagt agatgtagtg gatatattaa agaaaagtga tgcattgttg gagggacatt
     6061 ttttattatc ttcaggaaaa cacagtaata gatacgtaca atgtgcaaaa gtattaagat
     6121 ttccagaata tgctgctgag gtattaagta cagttgttga acaaataaaa gacttagata
     6181 tagacttagt agtaggacca gctatgggtg gagtaatagt ttcttatgag ttaggaagac
     6241 aattaggaaa agaagctgta tttactgaga gaaaagacaa tacaatggag ttaagaagag
     6301 gatttgaagt taaaaaagga gcaaagataa taattgctga agacgttgta actactggta
     6361 aatcaactat ggagacaaaa agagtattag aagccttagg tggagaagtt gtaggtgttg
     6421 catgtatagc agatagaact aatcatgata taggtatgcc tatatatagt gctataaaac
     6481 ttgatattca agtttatgaa tctgatgagt gtcctttatg taaggaagga aaattaccag
     6541 ttgttaagcc tggaagtagg gagttcaagg aattagggat gtaataaaaa cttaattatt
     6601 tatagtgtta cttaaaaaat gtaaattttt tagagtaaaa atttgtaata taacatataa
     6661 cttaaaaata aaaagcagtt agccttataa
//
LOCUS       DAELNY010000187         6662 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.190, whole genome shotgun sequence.
ACCESSION   DAELNY010000187 DAELNY010000000
VERSION     DAELNY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.190"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            18..869
                     /locus_tag="KPE55_003166"
     CDS             18..869
                     /locus_tag="KPE55_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG0494207.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="KPE55_003167"
     CDS             complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="KPE55_003167"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG0494208.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2840..4777)
                     /locus_tag="KPE55_003168"
     CDS             complement(2840..4777)
                     /locus_tag="KPE55_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494209.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4764..5531)
                     /locus_tag="KPE55_003169"
     CDS             complement(4764..5531)
                     /locus_tag="KPE55_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494210.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5819..6493
                     /locus_tag="KPE55_003170"
     CDS             5819..6493
                     /locus_tag="KPE55_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494211.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
BASE COUNT         2295 a         1002 c          787 g         2578 t
ORIGIN      
        1 cacaggaggt acattctttg aaaaacatta tttcagcaaa agaacttata tcaaaactag
       61 aaaaaaatga taatctagtt gtaatagatt gtagatttga tcttatcaat agaacttatg
      121 gtattgattc ttataaaaaa ggacacatca aaggcagttt tatcttagat atagacaagg
      181 atttatcatc tcctacaaaa gaacatggtg gtaaaaatcc tttacaagac cctttaatac
      241 taaaagaaaa attagaaaaa atgggtgttg ataacgacac tacgattgta acttatgatg
      301 atggagattt aaatggagct tgcagattat ttttccaatt aaaacatttg gggcttaaaa
      361 atgtctatgt actagatggg ggaataacat ctttcgtaaa ggaaggtgga gaattagaag
      421 agaaagtgaa tataccaagt tgtacaggta aggaaattag accaaatata aacaatgatt
      481 tagttgttcc aatggaatat gttaagtcta aattatacaa aaaagatact gtaataatag
      541 attgtcgtgc taatgagaga taccagggtt tagttgaacc tgcttactca aaagctggtc
      601 atataccaag tgcaaaaaat tatttttgta aggatttaat aaaaagtgac tttgaaaatg
      661 gctcacttaa agatatagaa tttttaaaaa aattctttaa agatttaaat aattatgatg
      721 aagtaatttt atcttgtggt tcagggatat ctgcttgtgt taacagtctt gctcttagag
      781 aactggatat acctcacaag atttatattg gtagctttag cgactggatt agttatgatg
      841 acaatgaaat caaaactggt caagaataag gctcataatc tatataaaat cagcatatga
      901 tagcttaaat tttgtaaagg cgtcagtcat tgtagacttt cgcctttact tattatttca
      961 tatactcttt aaatttatta tatatatttt agttgataat attaataaac tgcaatatga
     1021 ccatcacatc ttgattccgt cccacaaata taactttcct gttctgtttt aaatattatt
     1081 tgacctcgtc ccatgtcgac agtatcatga attactttta tttcatgacc cttttcaatt
     1141 aatgaatcta ttatatgctt tggcatctct ggttcaactt ctatttcttt tccttttatc
     1201 cactgccatc ttggagcatc tagcgcctct tgtggattta atccaaaatc aatcatattt
     1261 gttaacacct gaatatgtcc ttgtggttgc ataaaagctc ccattacacc aaatggacct
     1321 attgctttat cttctttacc taaaaatcca ggtattatag tatgataagg ttttttaaat
     1381 ggttttacta cattgtggtg tttagggtct aaattaaaat tatttcctct actatgtaaa
     1441 gcaatcccag ttcttggtat cacaattcct gaaccaaaat ttatgtaatt gctctgtata
     1501 tatgaaacca tatttccatc cttatctgca gtacaaaggt atactgtacc accacaatat
     1561 ggctctcctg cagttggata caaagcctct ttattgtcaa ttaataaact tctttttttg
     1621 gcatactctt ggcttagtaa ttcctgaatc tttaccttca tatgctctat atcagttaca
     1681 tatgttttag aatctgcaaa agctaatttt aaactctcta taattttatg tatgtcttct
     1741 acattttcta tatttccttc taattccaat tcttttaata tgttaagtgc cattaaaact
     1801 gttattccat gaccatttgg tgggatttca aatattttat atcccttata ttctgtactt
     1861 ataggttcta cccatgttgg ataaaaacac tctaagtcac tctttcttat agccccatta
     1921 aattttttag aatattcatc tattttatca gcaagtcttc ctctataaaa gctttcagcc
     1981 tgagtatttg ctatctcttc taatgtactt gcctgttctt cacatataaa tatatctcca
     2041 gcctctgggg ctttaccatc tttagaaaat gtatcaaacc atggtttgaa ctcttctcca
     2101 ataagttctt tttcatagag ttcatatgat tttttccata ccttagctac attaggagat
     2161 actacgtatc cctctcttgc atagtttatc gctggagata agcattccat taagctaagt
     2221 ttaccatatt ttttatttaa ttctgaccaa gctgctggta ttccaggtac agttactgct
     2281 ccaaacccat attttggcat ttctttcata ttattataat tttttaattc catattttct
     2341 ggagcaaagc cactagaatt taaaccatac aatcgttttt cttcctctat ccaaacaagg
     2401 gcatatgcat ctcctccaat cccattgctt gtaggttcta ctactgctaa tgtggcggct
     2461 gttgcaactg ctgcatcaac agcatttcca ccctttttta gtatttcaag tccagcttga
     2521 gatgctaatg gtgttgatgt tgcaactgct ccatttttag catacacgac atttcttatt
     2581 gatgagtatt tatgttggta ggcattaaac ttcataaatt tcagctcctc gtcatttttt
     2641 atttgatatt ctgcttagat ttattatata acatcaagta atgtaactga tacaaaatga
     2701 tataaaatat agactttttc ctaactttaa ttttattttt aaataaatat cttaaactca
     2761 attctagtat tttctatctg cacttaaata agttatttta ttcaaaacat atatctaatt
     2821 cttttaactt aaaaataatt tattctttta tcatgtttag agcatatttt aaagttaaaa
     2881 aataatatat aaaatatcct ataaaataaa tgactagagt tatgataatt ggtacaagta
     2941 atgaccttgg tataaacttt tgtgctatag acatagcaaa taaattatga actgtaccta
     3001 caactaatgg cattaaaaat ataactttta actgttttga tattattttc tcaatatctt
     3061 tattatttgc tccaagtttt attaacataa tatatctttc cttatcatca tatatatttg
     3121 ataattgttt aaataagatt atactacctg tacataaaag aaatactaca gacaaaaata
     3181 tgcctataaa cagcattaga ccacttaatt ttatcaagtt attataactt tcaatacttg
     3241 atgtaaaatt aaaaggataa gtaaatttca tattttcact tactatattt tttaatttta
     3301 aagtcaaatc cagttctttt ttttcatttt ttatatcaat taatcttatt aatctagaat
     3361 ttccatgtaa ttttaactga ttaaatacgt catcttttac tacaattaaa tcaaacgtag
     3421 cttgtgaatt tatttctggc tccaagatgc ttttttgtac tttaaacaga tttatatcat
     3481 actcatttgc atctttaata tgcaagttta ttttttcaat ctcaaagtct ttaaaaaata
     3541 tttttttgta tgaatcagaa acatagtaca cagattcctt tgaagttaat tcttcataat
     3601 tgttattctg atattgtctt aattttttaa aatcactttc tctaataata tctacagacg
     3661 ttctatcttc tatataacta tttccatttt tataatacat ttcaatattt atatctttgt
     3721 ctataagttc tattgtttta tcaaagatta tcttattatc ttttttatat ttatataata
     3781 atttgtcaat ttcgttattt acataagaat ttcctaaatt tatggtataa ctgtatttat
     3841 aattttcact tatgttccta tctatatcgt aataaagtga tatggtaaac cctagcatag
     3901 ttacacttgt agctattaat attgctatta cagccaatac actactattg ctctttaatt
     3961 taaatccaag ttcagaaaat gcaattaaat tctgtccctt ataataaaaa ctttttctct
     4021 tcttaacagc atcaataatc atatacatag ttgatgaaaa aaataaaaaa gttccaggta
     4081 ttattgtaac taatataaaa actgaaagca taatattttc agcaataaaa taactaattg
     4141 acattaaata agaaatagct attaataaaa caccaagtac acattttaac tttataaatt
     4201 ttttaaactt atacttttct ggatatttat taaataactc tataattctc ttatttctga
     4261 taactatagt atttcttatt ccaataacta taaatattat taaaaataca gctaatgtct
     4321 gaactaaggc ctttatactt aagttcatat ttactactat catttcattc attaatttta
     4381 caagcatcat agtcataagc tttgaaaata acagtcctaa aaatatacct attagtattg
     4441 caactatacc catcaaaaaa gtttcatata aaaatatttt tcctatctga tttttcctca
     4501 ttccaagtaa actataagtt cctacttctc gcctacgtct attgaaaaaa aatgagtttg
     4561 aataatatat aaataaaaat acaaatacca ctattacaat tgaacttgta ttaatactca
     4621 tcctggtttt ctctccaagt gaactcaacg cttcattata ctctatagat ttaaaactaa
     4681 aaaatacaaa tacactaaat acaattgaaa taaagtacat taaataatta aaaaaactat
     4741 tctttacatt ttgcattgca attctaaata aagtcatttt tattaactcc tccaattaag
     4801 gaaactgtat ttactatttt attatagaac tctctgttac tttctccctt tacaatttct
     4861 gtgaatatta ttccatcctt tataaagagt attctattgc aaaaactcgc acttatagca
     4921 tcatgagtta ccatcaatat tgttttattg ctgtctttgt ttaattttaa tagacaattc
     4981 aacaaatccc tagcagattt agaatctaat gctcctgtag gctcatctgc aagtactaat
     5041 gatggttttg ttgctatagc tctacataca gctcctcttt gcttttgtcc accagaaact
     5101 tcgccagggt atttatctaa tatagattct atatttaatt cttttgaaat ctcttttatt
     5161 ctattttcta ttactgatac tctttcattt tttaaagaaa gaggcaacat tatattttct
     5221 tttattgtaa gtgtatccaa aagattagaa tcttgaaata caaagcctag attatcacgt
     5281 ctaaaatctg ccaattcatt ttgcttcatt ttaatagtat tctttccatt tatataaata
     5341 tctcctgatg aaggtaaatc tattgtagaa ataatattta aaagtgttga tttaccagca
     5401 ccggaagacc ccataatacc aacaaattct cctttataaa ccttgaaagt tattcctctt
     5461 agagcttcat gttttgaacc ttttcttcca tactcctttt ttatattttc aacagataat
     5521 atttcttcca tagttaataa tcacctttca tcacaattag ttatttatca attctcacaa
     5581 atatattata tatattaaaa tggcttttaa acatttaatt atattaattt aaggttacat
     5641 tttcgtaagt attaattata tttcttcaaa tttattgcaa ttatgtttaa aatataattt
     5701 atcaagtaaa atatagttat attaatataa ttattttagg taataaggta gaaatatttt
     5761 aatttatcaa gtagaatata gttatgttaa tataactatt ttgaattagg aggtaagaat
     5821 gtttaaaata atggtagttg aagatgatgt ctcattgaaa aatatcatag caaagtgtct
     5881 tactaaatgg ggacatgatg ttcatcaaat agaaaatctt gaaaatataa tagaagagtt
     5941 caaaaactat aacccagaat tagttttatt ggacataaac cttccatttt atgatggttt
     6001 tcattggtgt aatgaaataa gaaaaatttc aaaagttcct ataattttta tttcttcaag
     6061 aaattctaat atggatgtaa taatgggtgt aaatttaggt gcagatgatt atatacaaaa
     6121 acctttttct gtagatgtct tagtagctaa agttaatgcc cttttaagaa gaacttataa
     6181 ttttgttgat aataactcaa atcagattat tcacaatggt gttactttag atttatcaac
     6241 tgcaacaata aactatgaag ataatactat tgagctaaca aaaaatgaaa tcaaaattct
     6301 ccatgagctt atgaaatata aggggcagat agtaagtagg aataaactca tgaaaaaatt
     6361 atgggataat gattggtttg tagatgataa tacattaact gtcaatgtaa atagaatacg
     6421 ctcaaaatta aatgaaattg gtttagaaga ttttatagaa acaaaaagag gattgggtta
     6481 tataatcagt taaaacaatt ctaagtaggt gaatttatga gctttaaaga ttttctcaag
     6541 gataaactag gttttttagt atacaatttt actttcttaa tttttacagt atcagttatt
     6601 atattttctc ctgtagatgt ttttctgact gatactattt tatatatatt agttgtaaat
     6661 gt
//
LOCUS       DAELNY010000188         6656 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.191, whole genome shotgun sequence.
ACCESSION   DAELNY010000188 DAELNY010000000
VERSION     DAELNY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.191"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(312..689)
                     /locus_tag="KPE55_003171"
     CDS             complement(312..689)
                     /locus_tag="KPE55_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0494212.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            1030..2142
                     /locus_tag="KPE55_003172"
     CDS             1030..2142
                     /locus_tag="KPE55_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0494213.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(2350..2826)
                     /locus_tag="KPE55_003173"
     CDS             complement(2350..2826)
                     /locus_tag="KPE55_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494214.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(3025..4650)
                     /locus_tag="KPE55_003174"
     CDS             complement(3025..4650)
                     /locus_tag="KPE55_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0494215.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4988..6007)
                     /locus_tag="KPE55_003175"
     CDS             complement(4988..6007)
                     /locus_tag="KPE55_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG0494216.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(6004..6573)
                     /gene="mocA"
                     /locus_tag="KPE55_003176"
     CDS             complement(6004..6573)
                     /gene="mocA"
                     /locus_tag="KPE55_003176"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG0494217.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         2316 a         1102 c          660 g         2578 t
ORIGIN      
        1 ccctagaaat aatcaaaaca aaaataaaat tatgcccttt atatctaaaa caattaattt
       61 acatgattaa tatacaaaaa ttctgacttt taaattcgta tacaatatca aactaaaata
      121 atattaatga caaacccaat ataaattaaa ttcaaacagg tttgatttta catcaaactt
      181 attgtattaa tattaaataa caagacaaaa atatttataa aaatcacaaa ttttacataa
      241 aactttatat ttgattcatt taactaatta ttgtaaaaac tatttatttt atgataatta
      301 actacaccaa attattcatc ttttttaata tccttaaccc atttttcaac agagttcaat
      361 tcttcttcat ttaccccctt tttgaacaag gatttcataa aactctcaat agaacttcca
      421 tgcatattat ctaaaaaagt ttttgtttca aaatgtaaat actcttcttc tccaataaga
      481 actgtataat gtgtatgtct atcaattttt cttgcactta agaattgctt atttactaat
      541 cttgataaaa gtgttagtgt tgtagtttgc ttccaattat atagtttttc catagcttca
      601 atgacatctt ttgatgctac tgtaaaatct gaactccaaa taaatttcat gacttttaat
      661 tctgctggtg gtatcttagt aatagtcata tgattttaca ctcctctcaa acttctacat
      721 aattaaatac tatattaata gacttttcga gtcaaaggtt ttatttaaaa tatctcatcc
      781 aaatttatta atacaaaaaa tttatatttt tattcaaaat taaacacaca atctattaat
      841 ataaagataa atatctattc tataagtata acagattatc aaattaaaat tctaatattt
      901 gacataatat atacattttt taaataaata tatacaatgt attaaaatgt atgcaactct
      961 atttcaaaat tattttgtga tatatatatg ttataataaa tgtataaaat tttgattggt
     1021 gggttatata tgaatgcatt tattagaaaa agaaataaaa actatgttgt ttacctggaa
     1081 tttgaagatg aaaagacagg taaaagaaaa caaaaaaata tgggtacttt tgataaaaaa
     1141 agggaagcaa gtaaaagact aaatgaggta aaagaaagct tatataatga tggtttttta
     1201 gtaccaagcg aaattacagt gtcagaattt ctattagatt ttctaaataa atatagtgaa
     1261 aatatatctt tagctacata taataattat gttagtattt gtaagaatta cattaatcca
     1321 tctattggaa aatataaaat acaagactta catccggttc acatacaaaa ctacatagat
     1381 aaactatctt ataaactaaa tcctcaatca attaagattc atataaatat attgaaactt
     1441 gcaataaaaa gagcttatag actcaaatta ataaaagaaa atgttatgga tagtatagag
     1501 gctccaagat ataaaaaatt taaaaatgag atttatgata gagaacaaat gataaaatta
     1561 ttagatttag caaaaaatac agaaatggaa ttacctatta atttggctat tggattaggt
     1621 cttagaattt cagaagtttt aggtctaact tgggataata ttgattttga agaaaataca
     1681 ataacagtaa ataagataac ttcccgaatt aatggttctg ttattctcaa agagccaaag
     1741 acagaaagct ctgttagaaa aatatcttct cccaaagaat taatgtcttt attaaaagaa
     1801 tataaaataa aacagaataa aaatttatta aaatcttcca ttagaaataa taataatttg
     1861 ttgtttttca ataagaaatg tgaacctatt gctgaagatg ttatgagtaa aaaatttaaa
     1921 agatttttag aaaagaatga attacctcat attagattcc atgatttaag acactcacat
     1981 gttactttac ttataaattc taaagtacct ataaaggtta tatccgagag agtaggacac
     2041 tcaaatataa gtactactct aagcgtttat tctcatgtac ttaaagaaat ggataaagag
     2101 gcttctgaca aaatttctga aagtttattt aatgctaatt aatattaatt tatcttagct
     2161 ttaaattagc ttagcttatt gagatagatt cattaatatt aaatgtatct atctcaaatt
     2221 aataactact taaataatct gttttatttt aatttatcgc ctcagttatt atactttttt
     2281 acgaaaaatt atactttaca ataaaaaaga tattccctta tagaaaatat ctttttaaat
     2341 actatattat tatatacttg ctaacattgg aatatctaac atcttcttca ttacatcatc
     2401 tacttttttt gctttatatt cattgtatcc aattaaatat cctttttcat atgaactttt
     2461 taaaatctct ttttcatata aattatcaga tttggcttct gtgacactta aattataaag
     2521 ccctttacac ccatcttcat aacctttatt ataccctttt tctgcaaaat agtttttatg
     2581 atttttttcg ttatgtactg aagatattgt tactccagaa ttttcttttg tactagcata
     2641 aggacaaaaa cctttattgt gttgatgtgc tgaatgtcct ccacaataat aacgtgttaa
     2701 ctctgattta tttttattat gatgacctcc tgatgaatta tgccctcctg agtgagcata
     2761 tgaaaaattc cctacactaa tcatagctaa actaattact acagctacta accttttctt
     2821 aaccataatc tattcccccc aaaatgttat atatttttat tatatcatct actatattat
     2881 atttaaattc ttaatataca aaatattact ctattctgct aataaacatg tttaaacaac
     2941 ataaaaaacc tcctaatatt tgattaaaaa tttacacact catgcatagc atgtctcaaa
     3001 tattaaaagg ttttatacta ataattattt atcttttcca tacattttag ctaattggtc
     3061 ttgtatctct ttactttcta aatactcatc aaagtccatt tttctatcca ttattccatt
     3121 tggagttatc tctataattc tatttgctat agtagatata aattcatggt catgagaagt
     3181 aaataaaagc actcctggga acttttcaag tcctttattt acagaagtta tactttctag
     3241 gtctaagtgg ttagtagggt catctaatac aagtacattt gcatttgaaa gcattagctt
     3301 actaagcata catctaacct tttctcctcc agatataact tgagcttctt ttaatgcttc
     3361 ttcacctgag aataacattc tacctaaaaa tcctcttata aaactttcac ttttttcttc
     3421 agagaactgt ctaagccagt caaccaaaga atattctact ccgtcaaaaa acttattatg
     3481 attttttgga agataatctt gagaagtagt tactccccat ttatactctc cactgtctgg
     3541 ttcaagttct cccataacta tgttgaaaag tgcagtagta gctatttcat ctcccataaa
     3601 tactacttta tcatctctat ccaatctaaa tgacacatta tcaagaacct taaccccatc
     3661 tatagtttta gttaaattgt gtacttctaa aacttcattc ccaatttctc ttgcaggtgt
     3721 aaatcctaca tatggatatc ttcttcttga tggttgtata tcttcaactt ctaatttctc
     3781 taactgtttc tttcttgatg ttgcttgttt agcctttgaa gcattagaac taaatctagc
     3841 tataaattcc tttaattgag ctatcttttc ttcagtcttt ttattttggt ccttagccaa
     3901 ttgtaaagcc aattgacttg actcatacca gaaatcatag tttccaacgt acatttgtat
     3961 tttaccaaaa tctacgtcaa ctatattagt acatatttga tttaggaaat gtctatcatg
     4021 tgatactatt ataactatag attcttctaa atccattata aaattattta accagtttat
     4081 tgatttaaaa tctaagtggt ttgtaggttc gtccattaat agtatttcag gcttaccaaa
     4141 aagtgattgt gctaacaata ccttaacctt ttccccacca actagttcct tcatttgttt
     4201 gtaatgcata tctttcgtta tgccaaggcc cataagtatt ttctcagcat tagtttctgc
     4261 atcccatccg tcaagctctg caaactcacc ttcaagttcg gcagctttta tcccatcttc
     4321 ttcattaaag tcttccttca tatacaatgc atctttttct ttcattatgt tccaaagtct
     4381 ctcatgacct cttataacaa catttaaaac agtttcttct tcatattcaa agtggtcctg
     4441 ttttaaaact gacattcttt ctttatcagt tatagatata ctacctgtat taggctctat
     4501 ttctcctgat aatattttta aaaaagtaga ttttcccgca ccattagctc ctattattcc
     4561 atagcaatta cctttagtga attttaagtt aacatcttta aatagttcct tatcaccaaa
     4621 tctaagtcca acacctgtaa cttgtaacat taatatattt tcctttcttt ttgctcataa
     4681 tttattttat cataaaactt tgataacatt tttattatat attatcacgt aaatagtata
     4741 ttttttatac taaatttgtg aaatatttat taaaataata ctaaaaattg taaatttata
     4801 aaaattaaca atctcttata ttatacttat aaagaaatca aattgcaact ttattcattc
     4861 ttttacacat tgaaaataag ttattataat ttctaaataa atatgctttt cttaaaacat
     4921 cattctagct gttttgtttc atatttaaaa agagtactta atttgtaaat actctttttt
     4981 aataacttta tattttagca aaattacatg caggataagt acatacatca cagttcatac
     5041 aaaggccacc atgaccatat ctagctatat catacttatc aagcttttca tcaattaaaa
     5101 ccctagataa aactatatca aatactgtag ttttgtgata cattgcgcat cctggtatac
     5161 ccataattgg aacttctcca taataagcaa gtaaaaacat agctcctggt aatattggtg
     5221 aaccatatgt cactaaatca gctccagttt cttttatagc tgttggtgta acatcatctg
     5281 ggtctacact catcccacca gtacaacaga taagttctgc tccttgactt ataaattctt
     5341 ttattgcact ctttataatc tctttatcat ctgggcatat agtctgtcct ataacttcac
     5401 aaccaaagcc ttttactttt tcttctatta cgggtccaaa tttatcaaca attctgctat
     5461 aaaacacttc atttcctgta gtaacaattc ctactttctt aggtttaaaa ggtactacat
     5521 ttactatttt tctattacca actacttttt ttgcatcttc taattttttc tcatctataa
     5581 ccaaaggtat cactcttgtt ccagctactt ttaaaccttt atttactata aaattattat
     5641 gaagagtagc catcattatt tctccaacac aattcaaatc aaaaagtgca tcaacatcta
     5701 tctttaatag accatcacat gctgctataa aatctatttt cccttctttt atttcactaa
     5761 aatctaaatt ttctccagca gttagttctt ttaaaaacaa tgcgccttca ttttcatgaa
     5821 ccattccttc tgacttttcc catacataca aattatcttt accaagagat aataacactg
     5881 gaatatcttc ttcttttact atgtggcctt ttttaaattt tcttccttta aattctccag
     5941 gtattatttg agttatgtcg tgacataaaa cttgtccaac agcatctata gtttttatta
     6001 gcttcataat ttacccccaa ttctctcata gtccattttt gtatcgatat caaataacat
     6061 ttctttagga acttctacat actttatttt tgatgaattt tttaaaatgg ttttaccttt
     6121 ttcatcttct ttaagtgcca acaattcttc tttcttgcta tatgggaata tgactggatt
     6181 tccacacata tctttgtatc ttggaattac tataaaatct ctatctaatt taaaagtatt
     6241 tataatttta tctatatata agttatcaat aaatggctgg tctcctacaa agaacatata
     6301 accatcacat tttgagctat ttaaaactcc taattttata gattcacttt gacctatatt
     6361 tgcattttta ttatctatat attcaaaacc atatttatta cacaataaca aaacctcttc
     6421 atattgactt actactacaa cttgatgaaa atcaatcttg ctaacttctc taaatacatg
     6481 ttctattata gaacagcctt tataatttaa taaaagttta tttgctccca ttcttctggc
     6541 aaagcctgat gccattatca ctgcatttat catatatatc atccttggta tttataatat
     6601 attgatagtt ctatgatttt tataatatct ctattgatat tatctatata tataat
//
LOCUS       DAELNY010000189         6644 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.192, whole genome shotgun sequence.
ACCESSION   DAELNY010000189 DAELNY010000000
VERSION     DAELNY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.192"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(483..2870)
                     /gene="cadA"
                     /locus_tag="KPE55_003177"
     CDS             complement(483..2870)
                     /gene="cadA"
                     /locus_tag="KPE55_003177"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG0494218.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDIVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2893..3258)
                     /locus_tag="KPE55_003178"
     CDS             complement(2893..3258)
                     /locus_tag="KPE55_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0494219.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3457..4305
                     /locus_tag="KPE55_003179"
     CDS             3457..4305
                     /locus_tag="KPE55_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494220.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4742..5830)
                     /locus_tag="KPE55_003180"
     CDS             complement(4742..5830)
                     /locus_tag="KPE55_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494221.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5955..>6644
                     /locus_tag="KPE55_003181"
     CDS             5955..>6644
                     /locus_tag="KPE55_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0494222.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDII"
BASE COUNT         2329 a         1109 c          722 g         2484 t
ORIGIN      
        1 ttatgtttgc ccttttctta aggacaagta atactatatc accttaaaaa taattcgtca
       61 atacttttta aaattatttt ttaaaaatat ttactttctt atattacata atttattgaa
      121 tggcagtcat actgttggtt tagagtattt taacacacac attttgttgt aaaaattaca
      181 actattaata taatatatat acaatcataa gtattataat ttttatgaat ctaatttaaa
      241 cttaatataa acttattaat aaaccttcta tgtctaaatt ttaaaattac aatataaaag
      301 ctctacacta aataaataca ttaaataaac ataatggata atgaatataa atttatatat
      361 gcaaagcgct ccaataaaaa ggtaccaata aagttaaaag aaacttattg atacctttca
      421 ttattacata cttcaatata taattataag ttttttaaat agtatggatt tacatttatc
      481 ccttatctca tagctctcat agcatttact actgctataa gtgccacacc tacatcagca
      541 aatatagctt cccacatagt agctactcct ccagcaccta gtatcataac tataattttt
      601 actcctagag caaatattat gttttgccac actatcttat tagtcttgtt tgctatttca
      661 attgctttag atatcttgct aggctcatct gtcataagca ctacatctgc agcttcaata
      721 gcagcatctg aacctaagcc tcccatagct ataccaacat cagcacgagc taatactgga
      781 gcatcattta ttccatcacc tacaaacgct attttctctt tttcagttct tccttcatat
      841 aaatcttcta atctatctac cttttcatta ggaagtaaat ttgagtaaac tgtatccaat
      901 tctaattctt gagcaatatt ttttgctact ttctcattat ctccagttaa cattacaact
      961 tcttttaccc ctatttcttt taagcttctt atggcatttt tactatcttc ttttacttca
     1021 tcagagataa ctatataacc tctatatact ttatcaacag caatatagac tactgtgcct
     1081 acttctttag ctgatgaata agatatattt tcttttttca taagtttctc attacctgct
     1141 aatataaaat tgccattatg ctttactcta attccataag ctgctatttc ctcataactg
     1201 tctattgtat ctaaatcaat ttttttatta tagtaactta atatggactt tgctattgga
     1261 tgatttgaat ttacctccgc aatagctgca tattctaata attcttcttc tgatatacct
     1321 tcaggattta acttagttac attgaacaca cctttagtaa gtgttcctgt tttatcaaat
     1381 actactatat ctacacttct taaagcttct aaatagttac ttcctttaat aaggatacca
     1441 tttttagatg caaacccaat tccactaaaa aaactaagtg gtattgataa aactaatgca
     1501 caagggcaag aaacgactaa gaatatcaat cctctatata accaatcact aaatacctca
     1561 cctgagatta caagtggtgg cacaaatgct attaataaag cagctattac tactattgga
     1621 gtatagtatc ttgaaaactt agatataaag ttttctgtct ttgatttttt aatgctagaa
     1681 ttttcaacaa ggtcaagaat cttagaaact gttgattctc caaaactctt tgtaacttca
     1741 atactcaata aagcattttt atttataaca cctgataaga tttcatctcc tttttcaact
     1801 tctctcagca ctgactctcc tgtgagtgca gaagtatcta acatagaaac tccatctact
     1861 actacaccat ctaatggtac tttttcacca ggttttacta ctattatatc acctacattt
     1921 acctcttctg gtgacaccac cttaacttca gaattaactt ttagatttgc atagtctggt
     1981 cttatttgca taagtgaagt tatagacttt ctggatttac caactgcaac accttgtaaa
     2041 tactctccta ctttatagaa aagcataacc cctactgctt ctgataactc ccctattgtt
     2101 aatgctccta ctgttgctag agccatcaaa aagttctcat caaatactct tccttttgat
     2161 atatttctta ttgctcttaa aagcacatca ccacctacta ttacataggc aactataaat
     2221 actatattgc taaactgact ttcataacct gtagctgttt gatatatacc aaatatatat
     2281 acaagtgctc caattataag tggaattaat tctttcttat ttgtatcatt tacagctcct
     2341 gatttttcag tagttctccc ttttactttg tcagtagacc ctacttgtat atcaagccct
     2401 ggctctgttg aatttattat ttcttttatt ttatttataa catcatcctc ttcaaagtta
     2461 ttacttatat ttactgtaag ttttttattt acaaagttta aattagaaga ttcaacttct
     2521 tttaatttag aaactttgtt attaatctct tctgcacaat gagcacagtt taatcctcct
     2581 aatataagct cttttttaat tgatgtttta ctagctgcat tttctttaga acttatttgt
     2641 atatcaagac ctggctctgt tgaatctatt atatctatta tcttttctat agtagtatcc
     2701 tcattaaaac tttcctttat atttactgta agttttttat ttataaaatt taaattggaa
     2761 gatttaactt cttgtagttt agaaacttta ttattaatct cctctgcaca gtgagcacaa
     2821 tttaatcctc ctagtataaa ttctttttta attgaattat ttgcttccat atatattcac
     2881 tcctctcatt tatcatttat aagtctctct aatatgattt aatccacaat caaatatctg
     2941 acttatatga ctatcatcta atgagtaata cactgttttt ccttctcttc taaactttac
     3001 taatctagcc tgctttaata ctctaagttg atgagatatt gcagaatgag tcatatttaa
     3061 taaactcgct atatcacata cacacatttc atttgcaaat aatgcatata atatctttac
     3121 tctagtagta tccccaaata ctttaaataa ttctgctaaa tcatataaca tttcttcatc
     3181 tggcatagtt gatttagcct ctgttactat ttcttcatgt actatattac aattacaatc
     3241 atttatctct tctctcattt ttaatcctcc attattttaa tatatgaata actgttcata
     3301 tgttcattat atgttatcat ttctaaattt gcaacaataa tttttattaa tttaatataa
     3361 atagaatgca catattataa acttttttaa tatatatatt attagaataa gtttttgtaa
     3421 acttttttat agttttctag gaggtgcaag tttaatatga gtaacaagaa aaaaaaggat
     3481 ttagatacta gttacatgcc ttacttagat ccagcgaact atgcagatta tattactaat
     3541 gaagaaattc cccaagatga attgagaaat gaccctcaaa tttctaatgg ttatacaaaa
     3601 atacctaata accagaacat aaataaccta tatcctaata atgtaaatca ttatcctaaa
     3661 agtagtaatt ctccaaacaa cttgcctaac acaaataata catccggaaa tatgaattac
     3721 aatacatctg ctacaaataa tatgcctaat aacatgaata ataatacggg tatgcctaat
     3781 aatatgtcta acaatatgaa tagtaatatg ggtatgccta acaatatgcc taataacatg
     3841 aatagtaata tgggtatgcc taacaatatg cctaataata tgaatagtaa catgggcatg
     3901 cctaacaatg tgcctaataa catgaatagt aatatgggta tgcctaacaa tatgtctaat
     3961 aatatgaata gtaatatggg catgcctaac aatatgtctg gcaatatgaa taacaatatg
     4021 tatggtaata caggtatgcc taataacatg aacagtaata tggggtataa tatgaacaat
     4081 actatgggta ttcctaactt ctcatgtaat caaaatatgc ctcctaatgt acttatgatg
     4141 ataccaggag ttatatgtca taatacaatg caaggtatgc ctgtagttat gcctagtact
     4201 atgcctccta atatatatcc tactccttat ggttctagta atatgtctgt acaaggtata
     4261 ccacaagcta ctaatattga ggaatttgat gaggaagaaa tgtaatatat atttttttaa
     4321 gtaagttgta taaaacatta ataattgacc ataataataa atgtattcct cataatattc
     4381 ccccaatatt attataggta tagaaagttg actgaatatg cgcttattcg gtcaacagat
     4441 attaacataa tctaataaga taaacataga aaaagtcaga taaaatttaa ttttatctga
     4501 ctttttcatt taaaaatgct ctcttgtgtt ggtgattcag aatagtatgg tactcgtatt
     4561 tgagactaat attggcaaac aacataaaga aaaatataaa ttttttataa taaactgtat
     4621 aaaattttaa taattgacca caataataaa tgtattcctc ataatattcc cccaatatta
     4681 tagaaataaa tttaagcgag attaaattca gttaatctcg cttaaataat gtctaaattt
     4741 attattccaa aaaatcatca aaatctaatg aacctgattc tgcaaaatca tataatgcat
     4801 tttgctcaaa gaaaaatttc tcttcaaatt taatatagtc gctataatta gattgactta
     4861 tttcattgat ataattgcaa tcattttcgt tataagtacc aataaaaaac ttatttccca
     4921 ttaagtaata tatagctttc atatcattat ttattatata tacattttct tttacatggt
     4981 cattattaaa attaaatcta gtaaagaata taccttcatt catattactt tttaacagaa
     5041 atggattttc tctataaaat actcctgcaa attcttcagg tgaatttaca ctgtaaacac
     5101 ttacaaattc acttttagaa atctcatcaa atacctcaaa atcatacata ggttctttat
     5161 ctgtcactaa tagagaatta attctaaaac cagtttcata acacttactt atgcaaaaag
     5221 caattttaca tatataatat ccatcattat cttcatataa agcttcactt atatatctgc
     5281 cttgaccttt atcacttacc acgtttttaa gtaatgttcc atttatatta gtaatgtgca
     5341 tatttgcaat ctcgtcactt ccagaaaagt actttaaact ttctctatcc catgctataa
     5401 atctcatggt catatagttt ataaattcaa cttcctcgtc aactctttta catattttat
     5461 tgtataattt ttctatagtt aaatctgttt tatagttttt tataatatca atatactcac
     5521 atttatctcc tggcaatagc ttatcataat aaagattctt attaccaaaa taagatacta
     5581 aaaataaaga ttcatccttc gttaattcaa ctctatctga accaaatcct cccatcaatc
     5641 tttcttcttc catataagct tcttcttgtg ttgggttatt taaactcaca taatccgcta
     5701 atcctaatcc ctctgcatct aataaaaaat attgacaaaa gtgattgttt tcatcatcta
     5761 tccaatttat tataagacct aaactgccca taagtctaga atttgtagcc tttgcaaaat
     5821 taaaattcat atagtcacat cctttaaact ctaaaatact tgttttttaa tatataaatt
     5881 attatacata tattttaaca acttagatac aatttataat ataatataag gaaatgatta
     5941 attgaggtgg tcttatgaaa aatatagcag ctttttttga tatagatggt actctttata
     6001 gagactctct tatggttgaa cattttaaaa aattaattaa atacgatata attgaccaaa
     6061 aaaattggta taaacatgct agagacactt ttatggattg ggataaaaga caaggtaatt
     6121 atgatgatta tctattagaa atatgtgacc tttatgttga ttcactaaaa ggattagata
     6181 aaacctgtat caactttaca agtgaccaag taattaaatt aaaagcagat agagtttata
     6241 aatacacacg ttcaaggatt gagtggcatc taaatcaagg acacatagtt atatttatat
     6301 ctggtagccc tggatttctt gttgaaaaaa tggcaaaaaa atataatgta accgattttc
     6361 taggaagtga ttatgtcttt gaaaacaata tatttacagg cacagttatt ccaatgtggg
     6421 attctataag taagaataat gctataaacg attttgttgt aaagtatgat ttagatttat
     6481 caaactcgta tgcctatgga gatactaatg gtgatataaa tatgctaaaa agagttggaa
     6541 atccaatagc aataaatcca acaaaggaac ttttatctca aatatcactt gatgaggaaa
     6601 tttctaaaaa tgttgagata atagtagaaa gaaaagatat tatt
//
LOCUS       DAELNY010000190         6493 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.193, whole genome shotgun sequence.
ACCESSION   DAELNY010000190 DAELNY010000000
VERSION     DAELNY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.193"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..728
                     /locus_tag="KPE55_003182"
     CDS             <1..728
                     /locus_tag="KPE55_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494223.1"
                     /translation="ETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNGEQQNINE
                     IKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDNLNKEKNA
                     NPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYEILDLKVD
                     YNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQVFDMKLKK
                     NIYEAELINGDISLQEKLFFVAN"
     gene            1094..2275
                     /locus_tag="KPE55_003183"
     CDS             1094..2275
                     /locus_tag="KPE55_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0494224.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(2417..3334)
                     /locus_tag="KPE55_003184"
     CDS             complement(2417..3334)
                     /locus_tag="KPE55_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494225.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            3587..4381
                     /locus_tag="KPE55_003185"
     CDS             3587..4381
                     /locus_tag="KPE55_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494226.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            4382..6442
                     /locus_tag="KPE55_003186"
     CDS             4382..6442
                     /locus_tag="KPE55_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0494227.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2525 a          697 c         1055 g         2216 t
ORIGIN      
        1 gtgaaactaa tatttcaggt tctaaagtca ataatatttg tatactaaag atagatataa
       61 aaaataagtc ttataaatta ttaaatagat ttaatttagg attacagaat ggagaacaac
      121 aaaatatcaa tgaaattaaa ttttctattg gaagtaaatt atacttagaa atagtaaata
      181 ataaaagtaa aaatggatat attgaaggac atgatagtta ctctaacttt attatatatg
      241 atatagaaga taacactttt aatataagta ataaatttat ggataatcta aacaaagaaa
      301 agaatgcaaa tccaaaggag attggcttat taagtaaaac taagctagat tatatggtaa
      361 aaaataataa attgaatctt atagttacgg atatgagtaa ttccataact aatcttgttt
      421 atgatattaa ttctaatgag attaaattag aagattatga gatacttgat ttaaaagtag
      481 attataatga agcatcatct ggagttagga aagttaagct actggatgat aagatatatt
      541 ctatttcagg tattaagaac agtatatcag gagaattaaa aggatttaaa gggattgttg
      601 atgtagtcgg aactaagcca gtaaaatttc aagtatttga tatgaaactt aaaaagaata
      661 tttatgaagc tgagctaata aatggagata taagtttaca agaaaagtta ttttttgttg
      721 caaattaagt gttagaatta attttagaac tttaaacttt tatatgtatc acagataagt
      781 ataagtaagg gagtgtctca aaataaactt tttagctcat gagacacttt ttttatatga
      841 aatcaaatat attttcattg tttagattta ttttgataat gaaaaaaaga ttatctttat
      901 tttaagaaag acatttattt ataattatat acaaaaatat tttttaatgt ttttgtgtat
      961 aattataaca atttaattta tattaatgca cttaaatttg aaatagaatg cttatagtgg
     1021 taaaattaat tattagaata ttagaaataa taaataaata atacattgta cttaaaaggg
     1081 ggagaaattt aagatgaaca aaaaatatat taaagcaggg aatttaattg atggaaccgg
     1141 aaatagtatt atgcaaaata agggtataat tattgatgga gatactatag ttgaaattga
     1201 agatataaga gatgatttaa atggatatga tgtgtacgat tacttagata aaactgttat
     1261 gccaggcatt atgaattgtc atgttcactt gacaatggag ccagttggaa atccaaagat
     1321 atattatgat aatgtatcag atgtagagct tgtagtaaat acgataaaac aacttgatgc
     1381 ttatttggat tcaggagtaa catatattag aagcttaggt tgtcccaaat atgtggatgt
     1441 acaacttaaa aatcttatag acaaagggtc tatagatggt cctggtatag ttacttcagg
     1501 tccagttata tgtatgacag gagggcatgg atattatttt ggtattgaaa gtgatggagt
     1561 agatgaatgt agaaaatcag ccagaacagt tttaaaaaat ggagtagatt gtgtaaagat
     1621 aatggcaact ggaggtgtaa caactgatgg agttgagcca gggtcaccac aattaacttt
     1681 ggaggaaatg aaggctgctg tagatgaagc tataaaagca ggtaaaataa ctgccacaca
     1741 tgcacaagga agaactggta taaaaaatgc agtgcttgca ggaattactt ctatagaaca
     1801 tggtgtttat ttagatgatg aaataattga tttgatgctt gaaagaggaa cttacttagt
     1861 tccaactgtg gctgcaccat attttattct aaatgcagga aaagaaagtg gaataactga
     1921 agctactctt agaaagtgtg agattgttgc cattccacat aaagagtcat tcctaaaagc
     1981 atataaaaaa ggaattaaaa ttgcagttgg ttctgatgca ggaacttctt ataatgaaca
     2041 tggaaaaaca tattatgaaa tgaaactaat gtcagattat ggtatggata atatggatat
     2101 aatagttgct gctacaaaaa cagcatctga acttttaaga attgataaaa attatggaac
     2161 tctagaaaaa ggaaagaaag cagatgttat agttgtaaat ggaaatccag tagagaatat
     2221 agacgtactt gcagatgtgt tagcagtatt taaacttggt aaaaaagtta gataaatagt
     2281 ataaaaataa aaaagagtgt ctaaagaaat ttttaaataa ttatagacac tcttttttat
     2341 tagtaataat taaatctttt atactttaga aaatagattc tattttatat ttagatataa
     2401 ctctttaaaa tcattcttat gcaatataat ctatagaatc tacacataca aagtatattc
     2461 ttgaatcatt agaatttcca agtactataa catcattttt cttacctaga acttcaacac
     2521 cagttagaac caaactacca gctactacag taacttgatt atttatattt gacatataaa
     2581 gattatctag gataccttta ttacaacagc aatcatcaca atcgcagttg caattacatt
     2641 ctttgtaatg ttttttatca agatagtaac ctaaagatgc tatgaaatct tcataatctg
     2701 caggtgtaat atcaaatgca acagctttaa gattacacaa tgatgctaaa tctacgtttg
     2761 ctaatggagt agatgcagca agtataaaat caattatagc agcaagtatt gcatctaata
     2821 tagccccaag gtctatcgta ggaggaataa ctgcatcaat aagtgcaatt aattcagcta
     2881 ctcctggtat agttcctata gtattaatta atccctcaag agttaaaggg actggaatag
     2941 gataagatac tctaccagct atatctatta aatcacagtt acaagcagaa aatctttcaa
     3001 aagtaccatc aagtccagat aaattatctt ttggaggagc tgaaagatct atacctacca
     3061 aattagcacc tactataaag aaatctgaga taaaagcaaa ttgattaaag tttacaaaag
     3121 gtcttagagc atcatatctt agaagttcta atgcttctct catacttttt ttacaacaac
     3181 attgttttat tttaggttct ttatatttat ttggataatt atagtatcta tcagctactt
     3241 gataattttc atagtatctt tcatttgtat ttggataatc ttcctcatat tcttgtgtaa
     3301 atctaaagtc ctctctacat ttattatttt ccactaaata cactcctttt atattttaat
     3361 ttaaatgtat agataattaa gcttatctat atagtaatat tatatttata atgtaaaaaa
     3421 atgttacaaa agatattata aacgaataaa aaataataat ttgccagata aacttgtaaa
     3481 tttttatgaa taatccattc taaaacaaaa aactgtgtat aaaaaaggtt tatatgttat
     3541 atttatataa atataacata tgaacgagaa aggagtggtt tgataaatga ttgaggtaga
     3601 taaattgtgt aagtctttta caagggttgt aaaagatgat aagaataaaa gttcaataag
     3661 aaagctaaag aaagtaaaaa ctaaaaaaga agaatttttt gctgtaaata atgtttcatt
     3721 taaagttatg gaaggtgaaa ttgttggaat attaggtcca aatggtgcag gaaagactac
     3781 attacttaga atgttaggtg gcatattaac accaacatca ggaagtataa atatttctgg
     3841 atatgattat tcaatagata gaaattcagc aaaaaaagaa ataggttatc tctctggaaa
     3901 tacaaaacta tatggaaggc tttctccgag agaacttcta acaacttttg caagtctata
     3961 tgagatgtca aaagaagata tagaagaatc aattgaaaat attgtaaaaa taatggatat
     4021 gagtgaattt atagataata gaattgaaaa tctatcaact ggtcagactc aaagaacatc
     4081 tatagctcgt tgtttaatac attctccaaa agtatatatt tttgatgagc caacactagg
     4141 tctggatgtt ttaagtagtg catctattat agattttatg aagaatgaaa aactaagggg
     4201 aaaaactgtt ctttactcaa ctcattatat ggaagaagca gaaactttat gtgacaagat
     4261 ttttatgatt cacaatggga aaataattgc atcaggaaca ccacagagtt taaaagagga
     4321 atctggagta aataatttga gagatgtatt cataaaactt gccagaaagg aggacattta
     4381 aatgagaagt aagattgtaa aatacatatt taaaaaagaa atgttagaca tattgagaga
     4441 taaaaagact ttatttatga tgattgttgt gccacttctt ttatatcctt tacttatgtt
     4501 attaatgatt caagtaatga atatgtctgc aagtagtatg aatagtaagg acattaatat
     4561 agcttttaat aataaaccta ataaggtttt agtatccatg attgaagatg ataatttaga
     4621 aaaagatact aaaaatactt caaatgataa aggcaaaata agagtgttag atgtagataa
     4681 ctataaaaag tcattagaag aaaataaaat tgatgcttat ataaagatgg aagaaaaaaa
     4741 atcatctata aattatcaaa tatatataaa ttcttctcaa gaaaattctt ctaatgcact
     4801 aaaaagaatt gaaactgttt tggataaata taaaagattt accatagaaa aaactttatc
     4861 tgaaaaagga ttagatgtac aagctacttt agaaccaata acatatagca ctgtagatgt
     4921 tgcaaagacg gaagaagtgg caggatactt tttaggtcag atacttccat ttatattaat
     4981 aataggagtt ttactaggtt caatatatcc agcaatagat gttatggctg gagaaaagga
     5041 aagaggtaca ttagaaacat tatttacact gccaatatca aacttagaac tggttatggg
     5101 aaaatatatg gcagtttctc ttagtgcaat agtaacagca attttaaaca ttttatctat
     5161 gggactgact atggcattta ttttagttag tggaggtatt agtagccaat ttggctttgg
     5221 aaactttaat tatggagact tagtcttacc aattataact actttaattt gcatatgtct
     5281 tttttcaatg gtagtttcag caataagtat gtgtgtatgt tcattagcaa aaagttttaa
     5341 agatgctcaa aactacataa cacctcttat gttaattatt atgataccat catatgtttc
     5401 tatgatacca aatatagtat tagatagagt gacagctgta atacctgtag taaatatatc
     5461 tttacttata aaaagtgtat tatcttttaa aagcgattta aatttaatag ccttagtttt
     5521 agcatcaaat gtagcctttg ttatattggc aatcatacta ctgtctaaga tgttcaattc
     5581 tgaggaaata ctttttggta atagtaagag tttctctttc ttagagaaaa gaagtaatat
     5641 aaaaaaaggg tctataccaa ctgtaagtga tggtgttatt ttatatgcag taggattagt
     5701 tctattgatt tatgttggtt ctttagttca aattaaattt ggtatggcag gtattataat
     5761 gacacaattc atgataattt ctttaccgtt attatttgca tactatatta aagctgattt
     5821 taaagaggta tttagcttga aagttcccaa attaaagcat gtgtttggga gcattgttct
     5881 ttggattggt ggatacatac ttattatgat tataacacag ctaattttat tcttatttcc
     5941 tcaaaatatg gaagttgctg aaagtttaaa taaggctctt tttatcaaag atagtgtttt
     6001 actgaattta ctagcaatag ctttattacc agctttatgt gaagaaatgt tttttagggg
     6061 atttatattt tcttcattta gtaaatcaaa agataaaaat aagtctatca agctagctat
     6121 aatatgcagt ggggtactat ttggtattat gcatatggat tttattagaa taattccaac
     6181 atcaatatta ggtattatat ttgcatatag tgtgtataaa tctggttcaa tatttgtatc
     6241 aatgttattg cattttttaa ataatagtgt agcagttgtg ttaaatcatt atacaactgg
     6301 aaatataacc aacttatata aatttgttga agttgatttt tcgaacttaa atgtacttaa
     6361 atttgcctta attcttctaa taagtcttat attaattatg ttaggattaa gagttttaga
     6421 tagaaagtct ttaagaaatt aagtataaag ttattatata gattaaaata ttaataagca
     6481 ctaaagtata tgg
//
LOCUS       DAELNY010000191         6484 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.194, whole genome shotgun sequence.
ACCESSION   DAELNY010000191 DAELNY010000000
VERSION     DAELNY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.194"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(91..468)
                     /locus_tag="KPE55_003187"
     CDS             complement(91..468)
                     /locus_tag="KPE55_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0494228.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            1373..1858
                     /locus_tag="KPE55_003188"
     CDS             1373..1858
                     /locus_tag="KPE55_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494229.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(2048..2587)
                     /locus_tag="KPE55_003189"
     CDS             complement(2048..2587)
                     /locus_tag="KPE55_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0494230.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(2580..2993)
                     /locus_tag="KPE55_003190"
     CDS             complement(2580..2993)
                     /locus_tag="KPE55_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0494231.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(3029..4150)
                     /locus_tag="KPE55_003191"
     CDS             complement(3029..4150)
                     /locus_tag="KPE55_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0494232.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            4749..6401
                     /locus_tag="KPE55_003192"
     CDS             4749..6401
                     /locus_tag="KPE55_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG0494233.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
BASE COUNT         2352 a          956 c          827 g         2349 t
ORIGIN      
        1 cttttttaat ataaactttt ttaattgtag acttttttta ttagttttta atttttatta
       61 gttttgttat tggttttatt gttataatac ttataatact gccattgctt caatttctaa
      121 aactgcatct tttggtaatt gagcaacttg tacgcatgac cttgctggtg gattttcttt
      181 aaaatatgta gcatatactt cattaatatc attaaaatcc tttaaatctt ttacgaatac
      241 tgtagtctta aatattttat ccatacttgt accagcttgt tctaatattg cttttacatt
      301 atctaaagaa gcttttgttg cttcttttgg gtcagatact aactcgccag tcttcatatc
      361 tattggaagt tgacctgatg tagaaactat gtttccaact tgaattgctt gagaatatgg
      421 tccaattgct cctggagctt tctctgtagc tattactact tttgacatga cttcacctcc
      481 tattattaag ttatatttac aggaaaataa aatttcctaa acaccaatat taatacgaac
      541 ctttagatta aatttcaaat ctaaaacttc aaataatcaa tacttagatg attatgttta
      601 tttgtgtctt gttaactttt attaaagcaa ttaacgtgcc aaactttatt tatatataaa
      661 attttgtgaa gtcattcaaa atagttactt ctgaattttt tcgtaaaaaa acgctctaaa
      721 attacttttt tatgatgtca atttttttct catatctaga aatttaccaa catgagaatt
      781 cttgttttga gaatttacag attaaagtta ttttttaacc ttaaattctg aacctcccat
      841 gactaaagtc acagggttcc tgcttcatag aattttgcat aatcaagtta ttacatatga
      901 aagaactata gttgacaatc ccactattat aaataataaa acctttaccc ataccttatt
      961 taccctagtt atcatagagc ctattgaaaa tataacagct aaatataata ataaccaaaa
     1021 aattatcaat atctaccact gttgctgaaa atccaaaaaa ctaccttttg atagtcttac
     1081 ttaacaatct aattaatctc ataataaatt tacaacttat aaataaattg ttatattggc
     1141 cgtatctcaa caactttctc ctttcctagt tttaaatctt ttatagaatt gtaccacgtc
     1201 taagtaaatt ttcatactat taagtcttat ttctaataaa tcattagctc tcattctatg
     1261 cccaataatg taagattgca gtaattttat tgattttcat agttttattt aatatactta
     1321 cattaattat tttcatatga aattattatt tatatagggg gattttttta atatgagcaa
     1381 aagtgaatta acagcagaaa caacagaaga aatgttagaa gtactaagtg gtaaagatta
     1441 tgatattgca tgtcatttac atgaacttgg taaatcatta gattgtaaaa ttgaaccaaa
     1501 aacaggtgct cgttcttaca aaatagtata ttcaactaag aaaccaaaac gtagcttatt
     1561 tactattgaa tgtaatgaaa aaaaatggag agttaaagca aatctttttc atctaaatac
     1621 atataaagat gctgtggagg aatgctctaa aactattaaa gatagtataa ctaaaactcg
     1681 tacttgtaag aaatgtaatt caaagtgtat tggaggttct tcgtttgaat tagatggaaa
     1741 gtcttacctg acttgcatag gaagtggtca ttattttgca aatatggaag aaatggattg
     1801 gaaaaaccta gaaaaattaa ttactaaaga aaataatatt atgcaggaat ctgtatagtt
     1861 ttttaagggt aattattaaa atatatgagt aacaacagat acttaaatag gcataaaaaa
     1921 ataaagggta taataaggat atagaataaa aataaatgat atagcacaaa gaggctgtct
     1981 caaaatacag ataagcctct ttttattgtt gaaatgataa gaatatattc aattttatac
     2041 aaacatacta cgccaactta cccatttcat atgcctgttt cattgcctta cttcccttaa
     2101 tatcacctac attccaagca cctacaccat atactatacc tttttcctta gaaccattta
     2161 gacatgatgt aaatcctcta aatccttcta ttgttctttc catatcagcc acattttcat
     2221 ctgctgctgt aacgataaaa tagaaatctt tatcacaaat ttcagtatat ctagagcaag
     2281 ttctatcaat catagttttc atttgtgcat tcatagtata aaaataaact ggagtcgcta
     2341 aaacaatcac atctgcccca atcatttttt caagaatttc tcccatgtca tctgtctgtg
     2401 aacaatttgc ttctttttca taacaagcac cacatccagt acaatagtta atatctttat
     2461 cttttaaaaa tatcttctcc acttgatttt tagattctaa taccccttgt ataaactggt
     2521 cacacaataa atcagaattt ccaccttttc taggactagc tgatactatt aatacatttt
     2581 tactcataag atactgcctc cattttctta tttctactgc tctttaatac tttttctttc
     2641 tcaataatac cttcttcata tctatcaatc ttaaaattta atcgttccaa agttttttgc
     2701 atttcttcta ttctttttgc tagttggttt ctctgctcta ttaaaagttc ttttctagct
     2761 ttgatagtac tgctcccttg ttgaaacata gctacatact caattaatgt ctcaatagaa
     2821 agacctgcac ctctcataca ctttatgaaa ttaacccact cacaatcttc ttctgtaaaa
     2881 gacctaattc cacttttatt tctatttaca ggtggtatta aaccaattcg ttcataatat
     2941 cgtaaagtat ccgctgaaag ctcgtatttt ttactaactt ctgaaatcgt catacatatc
     3001 ttctcctctt gatattgtat agattgaatt atttctcaaa ggtttctgct acattcttta
     3061 aagcatctat aattttaatg tgttgaggac agctttcttc acattgttta cattcaatac
     3121 aatctgaggc ctttccataa gttttagtat aattattata gtagaccatc tgtgttgaaa
     3181 atccagtatt aagagcttgt ttttctgcat tgtacaacgc aaaatagttt ggaatagcga
     3241 tattttttgg acagccttct acacaatact gacaggcagt acaaggaatt aaaattgatt
     3301 cgttgataat ctctactgct tcatctataa tattatattc ctcttgtacg aatggtttga
     3361 aattttgcat atatcctgta ttgtctaata attgttccat atttgacatc ccacttaaga
     3421 ccatcataac attatcttta cttgctgcaa aacgtattgc ccaagatgaa attgacatat
     3481 ctggattgta gtcattcaat aatttctctg ctttttctgg tatctttgca agtgtacctc
     3541 cttttactgg ctccataaca attacaggct tattatgctt ctctgccacc tcgtagcatt
     3601 ttctagattg tatgctttca ttatcccaat ctaaataatt aatctgtaat tgcacgaagt
     3661 ctacttctgg atgctcactc aagactttat ccaataattc tgcactatca tgaaaagaaa
     3721 aaccaatatt ctttattttt ccttcttttt tcttttcttc aataaactta aagctatcaa
     3781 attttttagc tacttcgtaa tgtgacacgc ctatattgtg taaaagatag tagtcaaaat
     3841 agtctacacc acatttttct aattgctcat taaaaatggt ttcttgctct tcttttttct
     3901 ttaaagccat taatggaagt tttgtagcta tagtaaaact ctctcttttg tgtcttttta
     3961 ctagagattc tctaagtgct atctcacttt ttcccatgtg atacacatag gccgtatcaa
     4021 aatatgtaaa tcccctttct agaaaagtgt ctaccatttt atttaaatgt tccatatcaa
     4081 tagttgtaac atcattttca tcttttattg gtaaacgcat taatccaaat cctaattttt
     4141 ttgcctccat tgctttactt ccccctttaa aacttgtttg ttttttctct ctactttaac
     4201 tattcttaca agtatataat tcttgtataa ttatacttta ctacttggag ttaacttcaa
     4261 gtcaatagat ttttttattt ttaagacaaa tattataaaa atacagttct ttaatattaa
     4321 tccattacat gtttctttca ccctccaatt ctcaaacttc acaaactcca acatgtaaag
     4381 aaatatatac tttaagaaat tcagtccatt aatattacta caaatacttt ttagatatgt
     4441 ttaaaaagaa atttcaccta aaagatttag aaaagattta gaaataggtc ttgtgtctat
     4501 tttaaaatat ataaaacagt ggtaaagact tatttataag ataaaaaatg aatagtgtat
     4561 acccttttta aaaagttttt tcctttttat atttgtgata caatgttaaa tataaaaagg
     4621 aaaattttgt attaaagaaa tcttatttgt tgattttgta acttcaaact cagcatcaat
     4681 ttgattttaa gattatattt tattaagtat gctaaataac aaatttttaa agattgtgag
     4741 gtaaaaatat gaataagaaa aatttagcta tattaatatc aggtatgatg tttttttcta
     4801 gttcttcagc aatttttgca gacaatacta caaagcaaga aagattaata ggtaaaacaa
     4861 gatatgaaac agcagtagaa gtaagtaaat tgggatgggt tcaatcaaaa acagccatta
     4921 tagttaatgg taattctatt caaagtgcgc tttgtgcaaa tccatttgct aagctaaaga
     4981 atgcacctat acttttagta aacaacaaca gtattgaaaa ttcaactaag gctgagctta
     5041 aaaggcttgg tgtagataat gtgtacattg tagatagtgg aaatagcatt tcttctaaag
     5101 tagaaaatga aataaaatca cttaacataa aaataaataa aatagtagga aacaatatat
     5161 atgaaatgtc tacaaatgta cttaaagaaa tagataagat aaaaaaggta gaaaatgttg
     5221 cagttgtaaa tggactaaag ggactatctg atgcagttag tatagcttct ccagcagcaa
     5281 tacataatat gcctataata ttagtatcag atgaaaacag caatgatagt agtataaatt
     5341 tcttaaacaa taggtctata aaaaaatctt acatagttgg aggaaataag attttatcag
     5401 acaatacagt aaagaaatat ccaaacagag aaagaatcag tggttcagat agaaacaata
     5461 ctaatgcaaa aattattgat aaattttata aagataaaaa aataaataat gtatttgtag
     5521 ctaaagatgg tattaaccaa gaaaatgcat tagtagattc attatcaata ggagtactag
     5581 ctgctaaaga aaattctcct ataatgatat ctaatggtag ccttgtaaat agtcaaaaag
     5641 aatttattaa aaataaagat atagatgaaa taacacaagt aggaggagga tataatcaaa
     5701 aagcatttga agatatttta aaactaaaaa atgtgaacat aagaaataaa actaaaaaaa
     5761 tatctttaat acaggctaaa gaaatattaa aacaagctct tccaggaagc aatattttag
     5821 tttgttatta tgatgaagat gataatgaat atgactgcaa agttataaag gataatatcc
     5881 tatatgagat agaaattgat gcctactctg gtaaaataat agaaattgaa aaagatgatg
     5941 acttaaatga taataattta ggtacacaaa atatatcatt ggataaagct aaatctatag
     6001 cattagatgc cattcctaat ggtaatttaa tctattgtaa atatgatgct gaagataacg
     6061 aatatgaggc taaagtaata aaaaataaga aaacatatga attagagata agtgcatttg
     6121 atggaagaat tgtagatatt gacatggaca atgattctga tgatgacttt gatgataatg
     6181 ataagaataa ctctaatact aatgacaaca gttctattat tagtgctgaa aaagctaaat
     6241 caataatgct agataaagtg cctggtggga aaatagtaaa atttaagtat gatgaagatg
     6301 acaaagaata tgaaggtgaa ataataaaag gaaaaactga atatgagata acaattaatg
     6361 caattactgg taaaatatta gaatttgaaa tagatgatta ataaaaaata tatattctat
     6421 taattattac aagcaataaa gccataaata ttgaaatttt caatatttat ggctttattt
     6481 tatt
//
LOCUS       DAELNY010000192         6480 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.195, whole genome shotgun sequence.
ACCESSION   DAELNY010000192 DAELNY010000000
VERSION     DAELNY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.195"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    94..305
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            468..1757
                     /locus_tag="KPE55_003193"
     CDS             468..1757
                     /locus_tag="KPE55_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0494234.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1811..2890
                     /locus_tag="KPE55_003194"
     CDS             1811..2890
                     /locus_tag="KPE55_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG0494235.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2911..4455
                     /locus_tag="KPE55_003195"
     CDS             2911..4455
                     /locus_tag="KPE55_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG0494236.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4618..5160)
                     /locus_tag="KPE55_003196"
     CDS             complement(4618..5160)
                     /locus_tag="KPE55_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG0494237.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5308..5592)
                     /locus_tag="KPE55_003197"
     CDS             complement(5308..5592)
                     /locus_tag="KPE55_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0494238.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5655..6419)
                     /locus_tag="KPE55_003198"
     CDS             complement(5655..6419)
                     /locus_tag="KPE55_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0494239.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2255 a          805 c         1125 g         2295 t
ORIGIN      
        1 aaactaaaaa atgcaagtaa ttgacttttt aagttgacaa aatattgcat atatattatg
       61 atataaatca taaattaact acaaatcaat attatatatt cgtagaagaa gaaagtagca
      121 taaaggaatt ttacagagag ttagatttgg ctgagaacta atattggaat ttatgtgaaa
      181 aggactttgg agaatactat tgtaattatg ttagaatagt ggtttaattc gccttaagaa
      241 aatgagtgag taaaattaat ttttactaat aagagtggta acacggattt atattcgtct
      301 cttggatttt tccaagaggc gttttttatt ataaacttat ttcagaaaat ttaaaactat
      361 atattaaatg aaaattataa aaatatgata gttaataaat ttataaatta gaaaattgta
      421 aaatatgcta aggtcagtag ttttacgatt taaatggagg gatgaaaatg gaagaagaaa
      481 aaattaacta tgctctggaa cagattccag agaatcaaaa acgtggttgg gtagctatgt
      541 tttcagtttt agtagcaata ggtgtggatt tatcatctgt tattttaggc gctgagttgg
      601 cacagagtat gccaatgaaa caagcaatat tgtcagtaat tgttggttca tttttttctg
      661 caattttgta tactacatgc tctttggttg gttcatctac aagtttatct acatctatga
      721 taacaaagta tgtatttgga gaagctggag caaaaatatt ttctctagtt ataggggttt
      781 ctctattagg ttggtttgga gttcaagtag gtttctttgc tcaaaatgca cagataataa
      841 taaaagatat atttaattta gatgtcagta tgcagatttt aagcctaata ggtggtctct
      901 tgatgatgag tactgcaatt tatggatata aggcaatgga gaagttgagt gtgtattcag
      961 taccattttt attagttctt atgatgctaa ctatattctt agcttttaga gcaaatggaa
     1021 tttcagttga tgataatatg aaatctacta tgacatttgc aggtggagtt tctctgtcaa
     1081 tgagtataat aatagttggt gcaattgtat ctcctgatat aagtcgttgg gctaaaagta
     1141 gaagagactg tgctctttca agctttttgg gaatacaatt tggaaatgct tttatgataa
     1201 tagtttcgat agtattagta aaatgtatgg gaacttcaga tattatgaga atttttatta
     1261 cattaggaat tgctatacca ggtataatag tacttacatt agcacaatgg actacaaata
     1321 caagtaatgt atattcagca tcattaagta tagctcttgt acttaagaag gctcctgaga
     1381 aagtattaac tatagtcctt ggtattatag caacattatt ggctgtattt ggaatatatg
     1441 aaggttttat aggattttta aatttacttg gaattgtaat tgcacctgtt ggtggtgtat
     1501 atacagcaga atattacata gtaaagcaag aacttaaagg ctttgataaa ggagtattat
     1561 ataaaccaat agttaaacgt tcaatagtgt cttggataat tggtatatta ataacttacc
     1621 tttcaacata tggatttata actcttacta ctatagcacc tttagatggt tttatagcag
     1681 ggtttgtagt acaatctatt attggaaaag tgctttgttc aacaaaaaat aagcaagaaa
     1741 tagacaaagc agtataaaga aatttgttta taaagtataa aataaattag aaatatgagg
     1801 agaaagtaac atgagatttt taacagaaga aagtgtagat aaaatagcag ttggtgcagc
     1861 agtgcttgga actggaggtg gaggtgaccc atatgtaggt aaattggttg caaaacaagc
     1921 cattaaaaag tatggtcctg taaaagtaat aagtttagat gaacttgatg atgatgcttt
     1981 ggtagttcca gtatctggta tgggttctcc tgtaattaca atagaaaaat tactttcaga
     2041 agtagagtta actacccctt tagaaataat ggagaaatta ttaaatagaa aagtggatgt
     2101 tataattcct atagaaatag gagggataaa ctcacttatg cctatagcag ttgctgctaa
     2161 aaaaggactt cctatcttag atgcagattc tatgggtaga gctttccctg aagctcaaat
     2221 ggtaactttt tatcttgaag gatatgaggc atctcctgcc gtaatgtctg atgaaaaagg
     2281 taattctgcc atcctttatc ctgtggatgg aatttggtca gaaagattag ctagaacttt
     2341 aactgtagaa atgggaggaa gttcttctat tagtgactat aatttaagtg gagctcaagt
     2401 taaaaaagct gctataggta atactcttac aatagctgaa actataggag ggtttttact
     2461 tgaaaataaa tctgattcaa aaggtgctgt tgagaatata ctaaaagaat tgtgtggata
     2521 caagttattt gaaggaaaag taattgatat taagagagag ttgaaaggtg gatttactag
     2581 aggacatgcc ttttttgctg ggattaatga gtatgatgga gagtattcta tattattcca
     2641 aaatgagaat ttaattgcca aaaaaggtga tacaccactt tgtattacac cagatttaat
     2701 agcagtatta gatttagaaa ctggatttcc aataactaca gagagaataa aatatggctc
     2761 tcgtgttatg gtagtagctt ttccttgtaa tgaaaaatgg agaacagaaa aaggaattga
     2821 gacagttgga cctggatact ttggatatga tgtggaatat aaaacagtag aagaattaca
     2881 aggaaaataa aagtttagga ggaaattttg atgtatcgtg taggaattga cgttggagga
     2941 acaaatacag atgcttgtat actggatggg gaattaaaag taattcattc agttaaggtg
     3001 gcaactacaa aagatgttga aactggtgtt tacaatgcct taaaaaaagt tatagatgaa
     3061 agtaagattg accattcatt aataaaatat gctatgcttg gtacaactca ttgtacaaat
     3121 gctattgtag aaagaaaaag acttaataat gtggctatga taagaatagg aaagccggcc
     3181 actactgcca ttcttccatt tatagattgg ccagaagact taagagaaaa aatagaatta
     3241 gattcaatat tggtatctgg tggatatgag tttgatggca gaaaaataaa tgaactttgt
     3301 aaagatgaag ttgtagagtt ttgcaataga ataaaaggca aagtggaaag tgttgcaata
     3361 agtggagtat ttgctccagt aaataaacaa caagaagaag aagttgcaaa atgggttaga
     3421 gaaattttag gggatattcc tatatcttta tcaaatgaaa taggtaatat aggtatatta
     3481 gaaagagaaa atggagctat tttaaattct gcattgtctc aagtaggtaa agctgtatct
     3541 ttaggatttg aaaaagcact taaagattta ggtatagatg ctcaaatata tttcagtcaa
     3601 aatgatggaa cattaatgaa tttagagtat acaatgaaat acccaatttt tacaattgga
     3661 tgtggaataa caaattctat aagaggagct tccttcttat caaaagtaaa aaatgcaata
     3721 gtattagatg ttggtggaac tacaagcgac ttgggtgtac tatataatgg attccctaga
     3781 gaatcatcta ttccagttac aattggagga gtacatacaa actttagaat gccagatgtt
     3841 ttaactattg gtcttgctgg tggtacatgt gtaaaaggcg aaaaagataa tataaagata
     3901 ggtcctgaaa gtgtgggata tagaataact gaagatgcaa taatatttgg tggagacact
     3961 ctaactttat cagatgttgt tacaaaatta gatatggcgt ttgaagatag aaaaagcaat
     4021 gttgaaaact tagataaaga gtattgtaat gaggtatata agaaggttat tttaaaatta
     4081 gaagatgctg tagaccaaat gaagactagt caagatgatg ttgaattggt tttaactggt
     4141 ggaggaagta taattgttcc tgaaaaacta aaaggcataa gcaatgttat aagacctcct
     4201 cattatactg ctgcaaatgc tattggtgca gctttaggac aaataagtgg agatgtagaa
     4261 aaagtataca gtttagataa aatgtcgaga gaagatgcca tagaagatgc aaaaaaagag
     4321 gctgtaaata aagctctaaa agctggtgct aaagaagata ctatagaagt attaaatata
     4381 gaagatgttc cacttgcata ccttcctggt aatgcacttc ttataaaagt aaaagttgtt
     4441 ggtgatttaa tttaaagaat aaatataagt atttccagaa ttatataaat atttctggac
     4501 tcatttttaa acttatttta tcaataaaaa cagcataact tttgactata ttcttataag
     4561 tttccgaaca gccgatatat acctattttt tgaggtttat cggctgtttg ttatctttta
     4621 tcgcattcca actatttttc ttaccgtatt tgctgtacga attgtaatct tatcacttac
     4681 atttgaactg gcagtctttg accaccaatt tgtcttttta taatctttac gactaaaaga
     4741 ccaaaagata gcatttttat aatgatatac tttttcatac tcttctttag gatttccaac
     4801 ctcatcataa aactcgtcat aagataagtc aggaaacata aaaataagat tatcatatat
     4861 ttctttgttg ttatctcccc accaattagg agcattattt agtatatctt ttaactcttc
     4921 ttgtgaaata ataaaaactg gtatttccaa ttcaaactgt ttttttatca ttaatcttat
     4981 tgtatttgag ataaccttgc tgtcatctac atcacttaaa aagacaacat taccactatt
     5041 aaggtatgta gaaacttctg aataaccaag ttctacaaag cttgctttta attcagacat
     5101 aattatcttg ttcttaccac ttatattgat acctcttaat aatgcgatat aacttttcat
     5161 ataataattt cacctctaaa cattcacatt tttctatgta gtacaaactt gaatttgcat
     5221 cttttataat tctttcaaaa cttcttcata tgtaattttt ttaatataaa aaaatcggtg
     5281 cttaatttgt accgattttt gtttttccta gtaaagaact cgtttttttg cttctgctat
     5341 agctctagca gcactttttg cttgcattct tttttcttct tcttttttaa attcatttat
     5401 tgtttcattt agattcttat catgttcatt tttttcttta tcttcgtttt ctctgtatac
     5461 taatatataa tctattattg attcgtttat tatctgagat atacttttat tttcattaat
     5521 tcctattctc ttaacttcct taagtagagt ctcatcaata tgaaccgttg tttgctttgt
     5581 attttttgcc accttaatca cctcttaaaa aaaattctaa cattaaaatt ttttttagtc
     5641 aataaatttt aatattaaaa ttctttaaat gttttatttg attttctaaa attttctatt
     5701 gtatttatca tcttattttt tatattttca cctagtacag aagactttag tctaggattt
     5761 tcgatatggt cataaattgg ttctaaaaat tcaacttcga caggaacaga gttaatttta
     5821 ccaataggtt ggtatccttc ataagtatct tttgaatttt ttattacgag tggaacaata
     5881 gggcattttg ctttatatgc tatctttaaa gctccacttc taaactccga aaccaatgaa
     5941 tttggttctt taatccatgt taaatctcct tctggaaaca ctgccatact ttgacctgta
     6001 agtatatttt gagaggcttg ggctatactc tttataccct cacgattgtt ttttctgttt
     6061 acatatacac atcttagtaa ttttgcccat tctttaaata taggtatatt tctccaaact
     6121 ggttcatctg caattacaca tccaattggt ctttctacac ttgctgttag tatgaagctg
     6181 tcaagcatac tagaatgatt tacaacaaat agaagtggtt cttttggcag tgtttcctta
     6241 ccaattatat taagctctat attcactata tctaaagatt ttttagcttg tttttgcata
     6301 aattcaaatt tttcctttaa gctaaattta tcaggattct ttttaatttt aataagccta
     6361 ggtatagaag atgtaaaacc tataagttga tatgtagcaa attttaagta attattcata
     6421 aaattatctc cttaaaataa aattatttat tactgtaaag attttaacat aaaataaaat
//
LOCUS       DAELNY010000193         6466 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.196, whole genome shotgun sequence.
ACCESSION   DAELNY010000193 DAELNY010000000
VERSION     DAELNY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.196"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            354..1628
                     /locus_tag="KPE55_003199"
     CDS             354..1628
                     /locus_tag="KPE55_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0494240.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1835..2152)
                     /gene="trxA"
                     /locus_tag="KPE55_003200"
     CDS             complement(1835..2152)
                     /gene="trxA"
                     /locus_tag="KPE55_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0494241.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2419..3171
                     /locus_tag="KPE55_003201"
     CDS             2419..3171
                     /locus_tag="KPE55_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG0494242.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3158..4039
                     /locus_tag="KPE55_003202"
     CDS             3158..4039
                     /locus_tag="KPE55_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0494243.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4265..5620)
                     /locus_tag="KPE55_003203"
     CDS             complement(4265..5620)
                     /locus_tag="KPE55_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0494244.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2492 a          936 c          817 g         2221 t
ORIGIN      
        1 aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta tttctaatta
       61 gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa taagtgacta
      121 tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa aatcaagtga
      181 gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat ctcaacaaga
      241 attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc ttgattaaaa
      301 cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt tttatgcaat
      361 tcatggatgt atctaaatac attgaaggtg caaaccttaa agttaaaact aaaagtggtg
      421 aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta aaaaacgtag
      481 tggataactt aaactgtgag attcctttgc ttacttatat taatgctcca ggaataatct
      541 cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa cttattgatg
      601 gagacgaaga aaaggattct gttatttatg tcaaaaatgc tacagaagga ataaacttct
      661 tagcaaaatt ttttaaagat gaaaatccag ataaatttgt tattaccaca gcaatggagc
      721 atatggctaa ttacctgcct tttaaagtta attttcctac aaaagttgtt ggaattacag
      781 aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat gctggtaatg
      841 tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact cctatatatg
      901 aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt gtgcaagtaa
      961 ttcagcataa acctttttca atgatgccac atgaatgtga tgagcatata gattttgttg
     1021 tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta ggacctaaaa
     1081 aattctttga aaaatttaat cctgtactat atggctcagg ttctaccaaa tttgtaaatg
     1141 ataacaaaat actatatgca aacgtacccc aacgttttga agcaggatac ccagagtatt
     1201 ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt ctaaacagaa
     1261 tttcaaaata cgaaagagaa ttatttttat atgctaaaga agaaatgagt aaaataccta
     1321 atataataat atatggagat aaatcaaata atgtaattat cccaaatata tcttttaata
     1381 taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt ggaatagaaa
     1441 ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt ataagtccaa
     1501 aagaagcata tgtcagattt atgagggaaa atcctgtagg tcttgttagg ataagcttag
     1561 gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa actttggctt
     1621 ttaaataagt tttttctatt cttttttatc aataaataga gagtagtttt gttttcacaa
     1681 aaaagcaaat tatatcaaca gtcaatttca ttagcaatta gacactcatt tgtaactata
     1741 caaactattt tacactatac aaacaaaaaa ctcattacaa tgagtttttt tgtttatata
     1801 tgagatttat tttaattact tcaatttact ttaattacat atatgattca attttgtttt
     1861 ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca tccttaaata
     1921 ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata ctttggtcta
     1981 tgtccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct tcatatattg
     2041 gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact actaaaccat
     2101 ctttattttc tacttcattt ataaattcat tattatttac tatttttacc ataatataca
     2161 tctcctttat tatcttcttg atattaatat actacgacct atataacagt tcaattaggc
     2221 acttttttgt aaccgcctat ttttaataga tttaatatta tgataaatag atttataaga
     2281 ttaaatattt ataagactaa atatttctaa tatattttat ttttttctta atatagttga
     2341 cattttttcc cttaaattat aaaatcaaat taataactac ataggtagtt actaatcttt
     2401 tatcaaagga gtttctatat ggataatatt attaatgaat tacaaaatat aggatttact
     2461 aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac aggatatgaa
     2521 ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac aaagctatta
     2581 aacaataata taattatcaa tgttggaact gaccctatta agtacatacc aataaaccct
     2641 aatgaattat taaaaaataa gaaaaaagaa tttgacaatt ctatggataa tctaaagaat
     2701 agcttacaga tattaaataa aagtaaaagt gtagaatacg tattaaacat aaaaggagat
     2761 aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg caaaacaaga aattataatt
     2821 tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc ttacaaaaaa
     2881 aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct tgataatatt
     2941 catattcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa aggaattatt
     3001 ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag tgaggccatt
     3061 tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat aaaacatgaa
     3121 atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta agattaaaca
     3181 agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg ttactgatac
     3241 ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt tatcagaaac
     3301 tgcatttgtt tcagagtcaa atattgcaga ttttaaagta caatttttca caccaggttt
     3361 agaagttgat ttatgtggtc atgcaacaat aggtactttc catgcacttt tggaagaagg
     3421 gaagcttaat actaataaaa gtatatttta tcaagaaaca aaagctggtg tattatctgt
     3481 agagcttaag aaaataaata atgaaaatat atttatgatg gaacagaata cacctgaatt
     3541 tgaaaattta gataattgca gaaaagaact tgcacaaatg ataggtgtgc atgaagatag
     3601 acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg tatttggtct
     3661 aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca aaaaatttag
     3721 tggtgataat aatataattg gtataactcc attttgtata gaaactatag ataaaaagtg
     3781 tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc ctgtatgtgg
     3841 aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag ataaaactag
     3901 ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg taaatataac
     3961 tagagaaaac aataacataa cttctgtaaa aattggagga agtgcttata ctgtcttaag
     4021 tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac tagctaacta
     4081 aagctagtat ttttatttgt tatttttatt taataatcat actctaacaa atattataat
     4141 tatatatatc ataatattgt atttagaagt aaaattttat ttttattatt aattagttat
     4201 aaatcaatct aatacaacat aattacagta gattaaccta taaaacataa taattgctta
     4261 ctgattattt atcgtcctca acaagtctac ttaatgtctt atcagataag aataataata
     4321 tcgctgcaac aaatagtatt actggaatta tagtatatac tttaatcata tcaaatttaa
     4381 gagtaaatgc atatatatat ccatatcctt taccagctat aaaagtagca aatgtccata
     4441 ctcccataag aacacctaga agcttcttag gtgcatactt acttacaaaa gagttaccaa
     4501 gtggtgagaa taacatttca ccaagactta aaagaatacc aaacatgatt atccatagta
     4561 tactagcttt agcagtttca ggagctccaa ctcctcttga aaattctgca ccaacaagca
     4621 ttaagaatgc aagacctaag aatattaatc caagacctgt ctttttaaat aaactcatat
     4681 ctccttgtgg tctactagca agtttaaacc ataatgctcc aagaactggt cctaatacta
     4741 tacatacaag tgaatttaat gagtcaaacc aagcaagagg aacatcaaat cctccaacat
     4801 tcatgtttac aaatgctgca ccataatcat aaactgcaag ataagttaga taccagaata
     4861 cccagaaaat tacagagaag aatgatataa gtataatagc ccaaattctc ttcttatcca
     4921 tgcttgtaag aggagactta tctttttctt caattttttc agcaactaca ccttctttaa
     4981 aaggtctctt tcctgcattc ccaaggaatc tccatccata tatgaaccat attgcaccta
     5041 ttacacaaag tacagctgct ataaagaaac attgagaaaa tccaagtaca ccattttttg
     5101 caaatgtttt aaggtaaaga ataccaactg ctatagtacc aataaaagaa cctatattaa
     5161 caaaagaata ttgtactgaa aatgctgtat caagttcttc ttgactatca aaaagctgtc
     5221 catttaccgc agatacgtta cctttaaaga atgcagtacc aattgaaact aagattatca
     5281 ttgcatttac catagaaact gtagttgcaa tagaaccaag atagtatcct acccccatga
     5341 taagcatacc tataggtata gtatatctag ctcctatcca tctatctgat atataccccc
     5401 caattatagg tgcaaggtat gtgaatgcaa caaggttggc ttgcattata gcagcttgtc
     5461 ctttatctat tccaagacca ccatgaacta tagctgttgt aaggaatata tatattaagt
     5521 actttgaagc ataatatgct gctctttcaa atgagaaagt tgttgagcag atataaaatc
     5581 ccataggaaa tttacccttc ttttttactg ttgagctcat aattttaaat cctttcttaa
     5641 ttgtattttt attttttacg ttttgtccaa attctttctt ttatgaaaga tataatatta
     5701 ttattttgta aaacaatttg taattattat taagataatt gcgcatttat ttgtagagta
     5761 catattctca tggaataaaa ttaatacact tcttaatcat aaatttaaat tatataaatt
     5821 cactattttc agatatagtt ataaattaat atattctcat ctaataccat gaaaaaacct
     5881 tcgccatatg caattcttat tttttacgtg atagtatttt acaaactaaa atcccccttt
     5941 tgtagcaact tatgtaatta cattttagtg cttttttaaa ggagtctcga ataaagcaac
     6001 atatattttg ttcaaataat gtttttattt attttttaaa tttatatatt acttcaccca
     6061 accaattctc aacttaaata actaaactac tatctattca cagattaggc ttatttctta
     6121 cacattagtt tataataaac actgtgaatc taaacataaa aactgttatt ttaaagtaat
     6181 tacccatata ttcgctttga tactatttat tgaggctata ctctttatgt ataatatatt
     6241 atttataaaa tacctcaaaa tatattattc ataaaatacc tcaaactgaa aactttttca
     6301 cgaatattta tttttcacct cccctttttt atttcaccat gtaaatcgta ccataaatat
     6361 gcacttatat gcaagaaaaa aatctattaa tcgacagtaa ataattagaa aaattcaaat
     6421 tatatttatc aattatatta tataaaatat ttcctatgaa aatata
//
LOCUS       DAELNY010000194         6442 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.197, whole genome shotgun sequence.
ACCESSION   DAELNY010000194 DAELNY010000000
VERSION     DAELNY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.197"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            268..1593
                     /gene="cdeA"
                     /locus_tag="KPE55_003204"
     CDS             268..1593
                     /gene="cdeA"
                     /locus_tag="KPE55_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG0494245.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1688..2482)
                     /locus_tag="KPE55_003205"
     CDS             complement(1688..2482)
                     /locus_tag="KPE55_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494246.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2483..3358)
                     /locus_tag="KPE55_003206"
     CDS             complement(2483..3358)
                     /locus_tag="KPE55_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494247.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3362..3730)
                     /locus_tag="KPE55_003207"
     CDS             complement(3362..3730)
                     /locus_tag="KPE55_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0494248.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3913..4572)
                     /gene="deoC"
                     /locus_tag="KPE55_003208"
     CDS             complement(3913..4572)
                     /gene="deoC"
                     /locus_tag="KPE55_003208"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG0494249.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4608..5795)
                     /gene="murJ"
                     /locus_tag="KPE55_003209"
     CDS             complement(4608..5795)
                     /gene="murJ"
                     /locus_tag="KPE55_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0494250.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5872..>6442)
                     /locus_tag="KPE55_003210"
     CDS             complement(5872..>6442)
                     /locus_tag="KPE55_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0494251.1"
                     /translation="LDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGS
                     LNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRL
                     ISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICP
                     SCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2130 a         1008 c          708 g         2596 t
ORIGIN      
        1 gttaaaatct atttcttttc agacaaaatc ttatatatat ataatagctt gtaaatctta
       61 agattatata catatatttc taaagatttt cttaagaaac agttagattt tactaatata
      121 agtttattaa ttagcactat actaatttat cttaatatga ttttttgttg attttaagct
      181 gtttatcgta taaataaaat ttttcttgac tatatagtta ctatatagct tattatatat
      241 agagagtaat aaaaggaggt aaaatttatg gaaaatttat ttacaagaaa attcactact
      301 tttgaatttc taaaatttgt ttctcctgca attatatcca tgatatttat atctttgtat
      361 acaataatag atggcatctt tgtatcgaca ttagttggtt ctgatgctct tgctagtata
      421 aatattgtac tacctataat taaccttgtt tgtggatttg gaataatgat ggcaactggt
      481 ggaggtgcta tcgtttctat acgtatgggt gaaaatagac aggatgaagc caactctaca
      541 ttttctttta tagttttgtt ttcattgatt gttgggattt tattcacagt aatctcatat
      601 ttctttatca aagaaatatc tattttgctt ggtgcaacag ataagttatt accatattgt
      661 ataacttatg gtaaagttat gattttatgt acaccatttt atattttaaa atttatattt
      721 gagtactttg caagaactga tggaaattct aaatttagtt tatttctatc agtcattggt
      781 ggtgtaacaa atataatatt ggattatgta tttattaaat attttggaat gggtctttta
      841 ggagctgcag ttgcaactgc tataggtatt attttaactt gtgttttagg tattatttac
      901 ttcttatcta ataaatctac actaaaatta agaaaaccaa aaaccgattt tagacttata
      961 agagatacta tgataaacgg ttcttctgaa atggttacag aattatctac aggaattaca
     1021 acattcttat ttaatgtagt agctttaaaa ttagcaggag aaaatggact tgctgctctt
     1081 actatagtat tgtatgctca ttttttaatg acatcagtct atctaggatt cgctgctgga
     1141 gtgtctccat taataagcta taattttggt gctgaaaaca gtgataaatt aaaagaaaca
     1201 tttaaacatt ctctaaaatt tatatttgtt tcttctcttt tagtgtttat tattgcttta
     1261 gtatttgcac catttattgt tagggtcttt gtaagtccag ataacacagt atttaaacta
     1321 gccttacaag gattaaaaat atttgcattt gcttttttgt ttgttggtat aaatatattt
     1381 gcatcaggat tttttacagc atttcacaat ggaaaaattt cagctattat atcttttagt
     1441 cgtgcctttg tttttataat cataggaatc ataattcttc ctcctatgtt aaacatgact
     1501 ggattatggc ttacagttcc atttgctgag gttataacca tatttatatc tattctattt
     1561 ataaaaaaat ataaatgtag atataagtat taaaaattaa tacttaaaat caacatttat
     1621 taaaactaat aaacctgctt taaaatacaa atttacaaat ttgattttta agcaggtttt
     1681 attttagtta taataagtca tttgcatact ctttaattgg tgcttttcta agtaataaaa
     1741 ttataaatat tatgcaaata actataattc ctacctttgt tccaaataaa ataaaaggat
     1801 tctgaaatga ataaataatt ttttctaaat acaatagcat tttatgcata tttttagttg
     1861 caaaattaac tatataaatt atcattaata tcggtaatcc tatcaatata gtaatactag
     1921 taacttttct aactctatat gtaaaagctc caagtaacac tcctataaaa cctatacata
     1981 tttcatttat acatatttgt acaaaataat ttaaataagt tagtggcata cttgatgttg
     2041 aaggatctat cagtgctgat atagatgtcc aaatcatatc tctctttagt aaaactgata
     2101 gttctatact gtaacctgtt ataatttcta taaataactt cgataaaaaa atcaaaacta
     2161 tacctaaaat agaaatcaca aagcttaaaa taattaaatc tatgacaata gcttttatac
     2221 aactttttct atctgctctt atacttaaag ctcctgaaaa gtctttattt gctatattta
     2281 ctccatacac aaatagcagt atagttatat atgttaaaat aggattcaca atcgtggata
     2341 aattagtttt agatgcttct ccaaaaatct tgagaataaa cactggcaaa atattcataa
     2401 caattcccac aatcataaat ataattatat acaatttagt atttttatta aaaaatttta
     2461 tatatggctt taattcattc aattacaatt cctccttctt agtcatgtta acaaacatat
     2521 cttgtagccc tatgtatcct acatcaatat ttgaattttt aagtattttt tcatcatttt
     2581 cactaaaatc accatagtaa aaataagcga ctgcatttcc aaatgccttt ttaggtttta
     2641 tattttttat acattctaat ttttctaact cttctttatt tccagtaata taatgagctt
     2701 tttgttttat gacatcaatt tcttcatcta taataatttt tccctcattt aatattatta
     2761 catgctccag taaatcatct atttcatcaa tcaaatgagt tgatattatt attgtccttg
     2821 gattttttat ataactatct aaaattatgt tataaaactc ttgtctattt actgcatcca
     2881 gaccaattgt tggctcatca aatatggtga tagctgaatt agaacaaata cctattatat
     2941 ttgaaagtat agttttcata cctcttgaca gttgtttata ctttttttta gtatttaaat
     3001 caaaatattt gcataattct tcttctaagc ctttatcata acttttataa aaactagaag
     3061 aaaattcaaa tatctgacct actttaaaat ctggactata aaattctttt tccctgacga
     3121 tacaaacttc ttccaaaact tttatatctt cttttgggtt ctttcctaat atttctatct
     3181 caccttcatt ttgaattaac tgatttacca ttatatttaa taatgttgtt tttccagctc
     3241 cattcttacc taataaacca tatatcttat ttttttcaaa gtttagagac atattattaa
     3301 aaatattttg cttcttaaaa ctgtgagata aattttctat cttaatagaa ctactcatat
     3361 attaatcctc cttgaaatta attataatat caaccaattc ttctctattt atttctaatt
     3421 tgttagcttc ttgaagtagg ttttttacaa aattatgttt aaaattttct tttctagctt
     3481 cttttataat agtttttgca ccatctgata caaacatccc tatccccctc ttcttatata
     3541 aaatattttt atcaaccaat atatttatac cttttaatac tgtagctgga ttaattttat
     3601 aaagctttga aagctctgtt gttgatggaa cctgttcttc ttcttttatt ccatttgata
     3661 gtatctcatc ttcaattgct cgtgctatct gaataaatat aggttcttca ttatttaata
     3721 ctaatttcat aggttcctcc tttcttcatc agttagttac tcaactaatt aactatataa
     3781 cttataatat cttgaaaaat attttctgtc aatactaaat aaaaaacaga gggaaaattc
     3841 cctctgtttt ttgttcttta tatttataat taaattttga tttttgtaat cctatcccat
     3901 aagttaaaaa ttttaataat ctgttgtatc ttcatttttt aatattttac atattgagct
     3961 agttcctaac ctttctgctc ctgcctctat aaatttttct gcatcagaaa ttgatttaac
     4021 tccccctgca gcttttattt ttacattttt tccaacatgc tctttcataa gctttatatc
     4081 ttctaaagta gctccacctg ttcccattcc agtagatgtt tttataaaat ctgaacctga
     4141 cattgttact atttcacaca tttttatttt ttcatcttca tctaaataac atgtctctat
     4201 aatgactttt aatactttat ctcctatagc tttttttatt tcttttattt cttttcctat
     4261 attatcataa tctttatttt ttatatctga aatgtttaca accatatcaa cttcatctgc
     4321 tccattttca atagcatctt ttgcttcaaa tacttttgta gcagtagttt gatatcccaa
     4381 agggaaccct atcactgtac atatctttat tttacctttt agatattctg atgctctttt
     4441 cacatatgat ggtggtatgc aaactgatgc tacactcata tctacagctt catcacataa
     4501 aattttaata tcttcccatg tagttgtagc ttttaaaata gtatggtcta ctgtttttaa
     4561 tatatgtttc ataaaatcct cctaaattta taattaatat aattagatta cttttttaaa
     4621 tgcaattttc tctttgcaat attcagtatc atatcaacct cttcaacttt aaaaatagtc
     4681 attagtagtg tataaattcc acctccaact ataactgaaa tagctagtga tacaaactca
     4741 ttaaatgttc ctactcccaa aattccaaat ataaatttat acgtaaagta tgataaaaca
     4801 cccataatta aagatgcaac taccgatttc aaagttgctt ttattatttt atcttgtcca
     4861 aaatatccaa cttttctctt taggttcaag aagagtaaca atatacatgc aattgatgat
     4921 gaactagttg caagagcaag accaccatga gccattggtt ttattaaaac taagtccaat
     4981 actatattta cccctacaga tataatccca tttaccatag gtgtttttgt gtcttgtaat
     5041 gaataaaaaa cctttcccaa aatatctctt aagccaaatg cagtcatacc aactgcataa
     5101 aaaattaagg cagttgctgt catttgggtt gccctcgcat caaaagcacc tctctcaaaa
     5161 atgattctaa caacaggagt tgcaaaaaaa atagaagcaa ctgaaatagg aatcatagaa
     5221 attattatca tatttatact ggatttaact gaactagtaa acttcttttg attattttct
     5281 gcacttaact tagataacat aggatacata acagatacta ttgaagctgt aaatgtccct
     5341 gtaacaaaac catttaatct atctgcataa gtaagtgctg gaatgcttcc tttaactaat
     5401 gttgaggcta aagttgtatc taccaatgag ttcacttgat taacagcaac acctataaat
     5461 actggtgaaa gtaatgctag taatttaata aggctatcat ctttaaagtt taagtagtat
     5521 aagtatttgt agttcgtttt tttaacaaaa aacatgtaaa agagcagttg aactaccata
     5581 gcaaccacag caccaattgg aagtatgtat ggtccaaata cggtacttaa cattattgaa
     5641 ataattatta ctatattata gggtatactt ccaaatccaa ctacaataaa gttctcttta
     5701 atttgtaaaa atgctgacat aacactcgta atacctatga atattattcc tgttctgaac
     5761 ctcccatgac taaagtcaca gggttcctgc ttcatagaat tttgcatact aaaatatgtc
     5821 ttactaattc tccacaggct atccccgtag ttcctacggt tcttacatac gttatttatt
     5881 ttgaattgtt attagtctta atccttcttt taatatattt atacttgcat ttatatctct
     5941 atcgtgagtt tcattacaac taggacaaat ccattctctt atatttaagt tcttaacttc
     6001 ctcgttttta tacccacatt tattgcatat ttgtgaactt gcaaagaatt tacctacctt
     6061 tacaatttgt ctaccatacc aattagcctt gtattctaat tgacgaataa actctgacca
     6121 tgatacatca gaaattaagc gagatagttt acgatttcta atcatattct ttacttgtaa
     6181 atcttctatg caaataatat cgttctcttt tattaattta gtagatagtt tttgtagaaa
     6241 atcatttctt tgattagcta tatgttcttg aagtcttgca acttttaatc ttgctttatt
     6301 tctatttaaa ctaccgattg tttttctcga taattctctt tgtaatttag caagtttgtt
     6361 taatgacttc ttaagatact taggattttc tataaattct ccacaacttg agatacaaaa
     6421 ttctttaatt cccaaatcta aa
//
LOCUS       DAELNY010000195         6395 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.198, whole genome shotgun sequence.
ACCESSION   DAELNY010000195 DAELNY010000000
VERSION     DAELNY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.198"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..1399
                     /locus_tag="KPE55_003211"
     CDS             206..1399
                     /locus_tag="KPE55_003211"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG0494252.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1605..2723
                     /locus_tag="KPE55_003212"
     CDS             1605..2723
                     /locus_tag="KPE55_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG0494253.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDIVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2854..3099
                     /locus_tag="KPE55_003213"
     CDS             2854..3099
                     /locus_tag="KPE55_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG0494254.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3086..3832
                     /locus_tag="KPE55_003214"
     CDS             3086..3832
                     /locus_tag="KPE55_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0494255.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENIFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4115..4990
                     /gene="hslO"
                     /locus_tag="KPE55_003215"
     CDS             4115..4990
                     /gene="hslO"
                     /locus_tag="KPE55_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG0494256.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFICECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5381..6181
                     /locus_tag="KPE55_003216"
     CDS             5381..6181
                     /locus_tag="KPE55_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0494257.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
BASE COUNT         2548 a          607 c         1262 g         1978 t
ORIGIN      
        1 tatattatat taatattaga aaaattttaa ataaatgttt tattctcaaa atatgcatat
       61 taatttaata aaatatacca aaatggaaat aaaaaataaa ttttatgaga atttaataac
      121 taataattaa tatatttagg ttataataaa tatgtgcgta aaatctaaca ataagcaata
      181 ataccgtaaa tttaggagga ataatatgga tactttaaag gaacttttta aaataggaag
      241 tggaccttca agttctcata ctatgggacc acaaagagca gcagagagat ttaagaatga
      301 aaacccagat gcagaaagtt ttagagcaat attatatggt agtttggcag ccacagggaa
      361 aggtcattta actgactata taattgaaaa aactatagca cctaaaaaag tcgaaattgt
      421 ttgggaagaa gatattataa aagattttca cccaaatggg atgaagtttg aagctttaga
      481 taaagataaa aatgtaacag ctgaatggac tgtatattct gtaggtggag ggactatagc
      541 agaagaaggc caaagaaaca gtaaaagcaa tagtatatat cacttagata ctatggatga
      601 gatagtaaaa tggtgcaaag aaaataataa gactttggta gattttgtgt tggaatgtga
      661 accaaaagac ataaaagatt atataaaaac tataaaagat gctatgagaa aatctattga
      721 tgatggacta agtacagatg aaataatacc aggcaagtta ttattaaaaa gaagagcaag
      781 tacattttat aatgcatata aaaaagataa gagcttctca acacttgtat atgcatatgc
      841 attagcagct tcagagcaaa acgcttctgg taatataata gttacagcac caacttgtgg
      901 ttctgctgga gtaataccag gaatattctt tgcaatgcag gatttctata attatgatga
      961 cgaaaaaata attgaagcac tacttgtagc tggaataata ggtaatataa taaaaactaa
     1021 cgcttcaata tctggagcag aggtagggtg tcaaggtgaa gttggagccg cttgttctat
     1081 ggcagcagca gcagtagcat acttaaaagg tggaactata gaccatattg aatatgcggc
     1141 agaaatagca cttgaacatc atttaggaat gacttgtgac cctgtatatg gatatgttca
     1201 aattccatgt atagaaagaa atgcaatggc agctcaaaga gcttatgatg cagctaatta
     1261 tgcattatta actgatggct cacattctgt ctccttagac caagttgttg aaacaatgaa
     1321 agaaactggt atagatatga tggataagta caaagaaaca gctaagggtg gacttgctaa
     1381 acatttcttc tcttgctaaa aataaaattt tccaataata attcctctgt tttgatttat
     1441 gaaacagggg aattattatt aatggtgaat tatttaataa tgcaaaatat ttttatattc
     1501 aatttagctt tttattttta attagtataa catgctatat aatattttaa ttataaagaa
     1561 atatagaaat attttttaat aaattagaaa ttggaggaga ttacatgaga gaggcatcat
     1621 atttacaaga acaactttat aaacatagga agtcattaaa tcctattgca gagattggga
     1681 gaaaagagta taagacttca aaatatataa gagattatct tgataaatta ggaattgagt
     1741 atgaagttta tttagaaaca ggtatagtag gtgtaataga aggtaaaaga cctaaaaaag
     1801 atattgcttt tagagctgac atagatggtc ttcatagtga agaaggggta aaacatttat
     1861 gtggtcatga tggacatgct actatattac ttggattaat tgagtattta aatgataata
     1921 aagaagactt aaatgataat atagtattta tattccaacc agcagaagaa ggtcctgggg
     1981 gagcaaaagc tttaatagat gaaggagtgc ttagaaaata caatattgat gaaatatatg
     2041 ggcttcatat atatccagag ttaccagaag gttatgtagg tacaagagag ggctatttta
     2101 tggctcaaat aggagatatt gacatagaaa tagtgtctaa aagtggacat ggggctatgc
     2161 cacaaaatgg tattgatggt atagtgatag cttcaaactt tgtaagctca cttcaaacta
     2221 tagtgtctag aaatataagc ccaatagaca atgcagtgtt gactattggt agaattgaag
     2281 ggggagctag aagaaatata atagctgaaa atataaagat ggaaggtact atgagatgtt
     2341 tcagcccaga agtgtatgag aaaatgaagc ttagagttag agaacttgct agagggtttg
     2401 aattggctta taactgtaag gttaatataa atattataga tgattatatt gctgttaaaa
     2461 atgacaagga tttgtatcaa gagtttgtag aggctattgg aagtgatatt gttgtagagt
     2521 tagagccact tatgatatca gaagattttt cttattatca aaaagaagtt ccaggattat
     2581 tttttatgtt gggttcaaga aacgaagaaa aaggatttgt aaatggtctt cataatataa
     2641 actttaattt tgatgaaaag atatgtgtaa atgcattgaa tgtttattca aaattattaa
     2701 aatacaaaga agcaatagat taggaagata agttataaag attaaaaagc ataaatacct
     2761 aaatttaaaa aaagtgtatt aataaatacc ccttacatat ttgtataaat ttattttaat
     2821 tgacaaaaat acaaataaga aggggtgatt tttttgaaag aaaaagtaca aagagaaaag
     2881 aagataacta atggcaagaa tactaaaata ttgacaaatg atgatataat gaaatatgag
     2941 atagcctcag aactaggtct tatggataaa gttggtgaac gtggatgggc aggacttaca
     3001 gctaaggaag caggaagaat tggaggtatg cttacatcac gcaaaaagag gcttaaaaag
     3061 attgtggagg agcagaataa aaaggatgag tcaatatagt gattttgcgt ttgtatatga
     3121 tgaacttatg aatgaagttg attataatgg ttgggttaaa tatatagaag atataataga
     3181 tagtgaaggt gtaaaagtaa aaaatatatt ggaattggca tgtggcacgg gtaatcttac
     3241 aattccattg actaaaaaaa attacgatat agctggtata gatatatctg atgagatgct
     3301 tagtgtagca agagaaaagg cagaaaaaga aggagtagaa ttagttttac ttcaacaaga
     3361 tatatcagag cttgattttg aaatatctga tttggactgt gtactttgtg cttgtgatgg
     3421 atttaattat ataacttatg atgatgattt ggaaaatata ttttcaaaga catatgaatt
     3481 attaaagaaa gatggaatat tcatatttga tataagttct ttttataaat tagccaacat
     3541 attgggaaat aacatgtatg gagaaaatag agaagatata gcgtatatgt ggcaaaatta
     3601 ctttgatgaa gaagagaatc taattgaaat ggaacttaca ttctttatta aagatgaatg
     3661 tggaaagttt gaaagatttg aagagataca ccaacaaaga gcatatactg aagatgagat
     3721 agttgatatg cttgaaaaat ctggatttaa ggatataaaa tattatgggg attttacatt
     3781 tgacaaacct agtgaagaaa gtcaaagaat cttctttgta tgtaaaaaat aagatattct
     3841 tgactatata agataattat taaaaataac catgatataa aaatatagaa aatgcagttt
     3901 tatagattaa aagtaaagtg atgatacttt atgactttta ttttttaata attataaagt
     3961 tctatagata aaaattaaaa tatagtaact attacttagt aaaattaaaa taaaatgggt
     4021 tttgcccaag gttaagatag ataagtattt atagataaat taaggtagta atataataaa
     4081 aataagacaa ttaacgggac atggaggaat attaatgaga gattatgtac taagagcgac
     4141 atcaggaaat ggtcaagtta gagcatttgt cgcaactact agaaatacag tagaagaagc
     4201 tagaagatta catgaaacta caaaagtagc aacagcagca cttggaagaa cattaacagc
     4261 cacatctata atgggattaa tgatgaaaaa tgatagtgat aaattgactg taataataaa
     4321 aggtggaggt ccaattggaa ctattatcgc cacatcagat tctaagggaa tggtaaaagg
     4381 atatgtagga aatccacaag tagaggttga agactatcca aatggaaagt taaatgtagc
     4441 agcagcagta ggtacagaag gtgttgtaaa agttataaaa gatttaggtc ttagagaacc
     4501 atataatggg acttatccat tggtaagtgg agaaattgca gaagacttta cttattattt
     4561 tgctgtatca gagcagactc catcagtagt agctttaggt gttcttacaa aagaagatga
     4621 agtagagttt gctggaggat ttatagttca attaatgcca gatgcagaag aagagactat
     4681 tgctaagtta gaggaaaatg tagctaaatt accttctata actaacatgt taaaagaagg
     4741 aaaatcacca gaggatatat taaatattgt tttggatggt ttggaaccta aaatacttga
     4801 tacctgtgaa gttgggttta tatgtgaatg ttcaaaagaa agagtaaaaa ctgctttggt
     4861 tgcaattggt aaaaaatcat tggctcaaat aatagaagaa gataaaaaag cagaagttgg
     4921 ttgccagttc tgtaataaga agtatatgta tagtgaagaa gagttgcttg aaattttaaa
     4981 ggaaatgtag actataaaaa tagttgtatt gatataagta atgttatagt gatatagtaa
     5041 agtcgtattg atatagtaat taaagtataa tttattaata atagacgtat ttatattaaa
     5101 aaataagttt aataaatttt taagcttaat aaattttaag aattaagaag ctttctggct
     5161 gtaatgtttg caatgttgca gttagaaggc ttttttattt tattatagtt cgtgtcaaag
     5221 attgtatgaa acaataaaat attgtaaaat aatttgtata tttagacctg attggcacaa
     5281 aggtttataa ggtatttgag ttgattatgc gaaattctat gaagtagtaa ccctgtgact
     5341 ttaatcatgg gaggttcaga agaaggagaa aattttaaat atggaaagta aaacgaagta
     5401 cataggaata agtatattaa tattattgat gatattttta attgcaaaga attttaacgg
     5461 aaaacttgaa atacctattc ctgagagtaa tagtagaaaa tttaaatcag cagcagtgtt
     5521 ttatcctcaa catcaagatg atgaggtttt atggggagga agtgccattg tagatgctat
     5581 aaaacagtgt ggtgttgata atgtatatgt ggtattagta tcagatggtt ctggagttaa
     5641 cgttttcaaa gctaacacaa aatttagaaa tttaactagg aagcaaaagg aagagcttag
     5701 aaataacgag tttaaatcag ctttgagaga gcttggagtg aaacctcaaa atgtgattat
     5761 acttgcagat atagataaaa aggaaggcac acattatgaa ttaatggaaa agactatatt
     5821 ggattttgaa cataagttta aaggcaatgt gacccatata gcccatcact ataagtatga
     5881 tgaccatata atgcatagaa agaatgggga agttttaaag aaacttaaac gtgaaggtaa
     5941 gataaaagat gacttgtatt ttgttaagcc aaaatatgta aagtatatac caagaaatga
     6001 aagagctatc tataaagcaa tggattttga tgactacaat aaggttaaga gtgcttgtta
     6061 tgaatataag gttgtaaatt caaagcgtaa aatgtatggt ataggatata tttcggcaca
     6121 tagttatttt gaacatcttc taaatgaccc tgaacttacg tctgtattaa gtgaagatta
     6181 atttcttata agtagtaagt tatgtttgat aagttgattg gaattttcta tagttttgaa
     6241 ggtattggaa atttagtttt atttaaagat ttaaagatag aggtttttag tgtttaaaat
     6301 tttaaagtta agttgaataa ttgtatagaa ttatttgtga aagaagaaac agtgtaaaac
     6361 atagtatctt atttatggtt ttaaaatcat aggca
//
LOCUS       DAELNY010000196         6379 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.199, whole genome shotgun sequence.
ACCESSION   DAELNY010000196 DAELNY010000000
VERSION     DAELNY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.199"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(63..959)
                     /locus_tag="KPE55_003217"
     CDS             complement(63..959)
                     /locus_tag="KPE55_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0494258.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRIDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1100..2527
                     /locus_tag="KPE55_003218"
     CDS             1100..2527
                     /locus_tag="KPE55_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0494259.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2723..5317)
                     /gene="clpB"
                     /locus_tag="KPE55_003219"
     CDS             complement(2723..5317)
                     /gene="clpB"
                     /locus_tag="KPE55_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG0494260.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5354..5950)
                     /locus_tag="KPE55_003220"
     CDS             complement(5354..5950)
                     /locus_tag="KPE55_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494261.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5967..6371)
                     /locus_tag="KPE55_003221"
     CDS             complement(5967..6371)
                     /locus_tag="KPE55_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG0494262.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2031 a         1036 c          686 g         2626 t
ORIGIN      
        1 tatttattaa gaaaaactca aagatttaaa aatatatatt ttctataaag tatatttatg
       61 gattatttca tatcttcttt taaaataaca ttactaactt ctctatgttc atttctatac
      121 ttagttggag atatattata attcctttta aataagcgac taaaataatt tatgctatca
      181 aagccacatc tcatagcaat ttctgtcata tttaaatctg tttcctttaa ataatatata
      241 gctttatcaa tcctgattcc atttataaaa tcgattgtag tcttacctgt tatttgctta
      301 aatgttctac aaaagtgatg tgcactcaag ttcacacgac tagcaagttc ttttaatgta
      361 attttatcca tatatttttc ttctatcaaa tcaaagatac attcaaattt ttctaaagaa
      421 tactttcttt ttataaattc atcttcagaa tatattttat caatatgact tcttaataag
      481 agtactatta attgatagat attagctttt atagataact catatccaac ttctttattg
      541 tgatattcat caagtatagt tgtaatacat tttaaaatca gagaatcatt ttgaattaaa
      601 ttattaaata caatatgatt taaagccaat ggtgctaagt acttggtttg taacaaatca
      661 acttgattac taaaaaggaa tgtgggttca atacgaatac tatagaattt taaattatct
      721 gataagcttt ctaaataatg taattcatta ctgtttgcta ttacaatatt tccaggcact
      781 accttaaaat tatccaatcc acattcaaga tatgctatac catcaataaa ataatatatt
      841 tgtagatgct catgccaatg tcttttaaag tttgctcctt ttttaatgtc atcctttaaa
      901 aatatatcta taaagttttc ttgacctttc ctgttaaaat atgtattttt atccttcatt
      961 attcccccct cagcaaaata gtgctaatta taagcaatat agttattgta ttcattattt
     1021 catatcgcta ttatataaaa tgtaactatc attgtaaaca ttaacataat gcagtcatta
     1081 taataaggag gtaataaaaa tgacaaactc ttttaaaaaa gatgataata gaaaaaaaat
     1141 cacagtttta tttgtagtca ttgccatgac ttttatggca acattagatt ctagtattat
     1201 aaatgttgct ctcccagttc ttgcaagcaa actaaatgta tctctagcat ctattgaatg
     1261 ggtaatagca agttactcaa ttattatttg ttcaacacta ttattttttg gaagacttgg
     1321 agatattatt ggtaaatcaa gagtatttca agtaggtact attctcttta catcagcttc
     1381 attactatgt ggattaagta attctttaac tttacttata gtttgccgct ttattcaagg
     1441 aataggcgct tctgcctaca tggcaaacaa tcatggaatt atcacagaac tatttccaaa
     1501 agaaagcaga ggaaaagccc tagggattct agttactgca gttgctattg gaaatatggt
     1561 tggaccatct gtgggaggat ttattttgtc aatatttgat tggaatgtta tattttttat
     1621 aaatattcca atcggtctta ttgtaatctt tttaaataca aaatttcttc ctaatagtaa
     1681 aaaatcttca gaaaatatgg ataaaactgg tgctatactt caatttttag gaacaacatt
     1741 attttttagt gctctaatta gtgctcaaca aacaggatta ctaaatccat atatattaat
     1801 tgcactatta ttatcaatta tttttattat attatttttg atattagaaa aaaaacatcc
     1861 tcaaccgctt cttgaccttg aaatttttag aaattttaaa ttttcattga atttaatttg
     1921 tgctcttaca tcattcattt gtattgcatc atcaagtatt ttaattccat tttatttaca
     1981 aagtactatg aaacttcccc ctattcaagc aggtttattt atgatactat cacctttgat
     2041 tttagccatt ttttcaccta tatttgggaa tatatcagat aaaattaagt cagaaaaaat
     2101 tattttaatt ggtcttctcg ttatgagttt tggattcttt ctaatgtcaa gattgaaaga
     2161 aagttctgcc cttattttat ttgtaattta tatatcgata atatcaatag gacaagctat
     2221 atttcaacct gctaacaatg ctttgataat gtcaagctgt tctagaagta agctaggagt
     2281 tgtaggaagc ataaattccc ttgtaagaaa tttaggacaa gttataggta ttactatttc
     2341 tacaacactt ttatataatt ttatgagtat taaagctgga tatagggtca atgattatgt
     2401 gattaataat gataaaatat ttgtatttgg aatgagaaat gtttatatta ttgttacact
     2461 tgtttgctta ataggtgcaa tattaattgg cttttattta tttaaatata ataaaaatga
     2521 acaatagttt ttataattat attcaaatta tgattatatt ataagaaatt tactaaaata
     2581 cttcctttga gttaaattta atttggacat attgacttaa aaatgtttta ttaatttgat
     2641 aaaatatttc ttttcctata gtttataaat aaaaatcagc tgagcaaaat atttagtata
     2701 ttatgctcaa ctgattcttt tattatttaa ctaaaacttc aatattttca tcttttccat
     2761 ctataactat agtacatcca ttatacacat ctccagcaat aagtttcttt gcaattatag
     2821 tttctaatgt atttcctata tatctcttaa gaggtcttgc tccatataca gggtcatatc
     2881 cttctctcac cataacatct tttgctgaat ttgtaacttc aatagtaata tctttttctt
     2941 ttaatctatt ttttaaatct ttcataaata tatctatgat tttcttgata ttttcttggt
     3001 ctaaaggctt aaacattata atatcatcta ctctgtttaa aaactcaggt ttaaatctat
     3061 gcttcatttc attcattact aaattgttag tagtctctgt tatatttcct cctgcttcta
     3121 atagataatt acttcctata ttagaagtca ttattataag agtattttta aagtctactg
     3181 tcttaccttt attatcagta agtctaccat catctaatat ctgtaagaac atgttaaata
     3241 catcctcatg tgctttttct atctcatcaa atagtattac agaataagga gcacgtctaa
     3301 ctgcttctgt aagttgacca ccctcttcat atcccacgta ccctggagga ggtccaacta
     3361 gtcttgatac agcatgtttt tccatgtatt cagacatatc tattcttatt atattatctt
     3421 cactatcgaa taaatttcta gcaagagttt ttgcaagttc agttttacca acacctgtag
     3481 ggcctaagaa aataaatgaa ccaattggtt ttctttcatc ttttaatcct gctcttgcac
     3541 gtattactgc atttgaaact gctgtaactg cttcatcttg tccaataact cttttatgaa
     3601 gttcatcttc tagtttaagt aatttttctc tttctccttc aactagttta gttactggta
     3661 tacctgtcca ttttgataca atgctagata tttcttcttc agtaacttct tcttttaaaa
     3721 gagaattttc agaaccatca ttcatatttt cttcgtactg ctttatttgt tcttctaact
     3781 taggtatttc accatattta acctctgctg ctttgttgaa atcatattct ctttcatact
     3841 tttcagcttt acctttagct tcgtctaatt gagcctttaa gtttttaata tctaatattt
     3901 gactcttttc tttttcatat tttgctgtca tttcatcatt tttagatttt aactctgcta
     3961 tttctttttg aatatcatcc aatcttgatt tacttttatc atcattttct ttaagtaatg
     4021 cttctctttc agtttctaat gtaaataact ttcttctaac aacatctaat tctgttggca
     4081 atgaatctat ttcactacga atcatagctc cagcttcatc aattaaatca attgctttat
     4141 ctggtaaaaa tctatcttgt atatatctat gagaaagttt tgctgctgct acaatagcat
     4201 tatcatgtat tcttactcca tgatgtatct caaatctttc ttttaaacct ctaagtatag
     4261 atattgtatc ttcaacagta ggttcttctg caattacagg ctgaaaacgt ctttcaagag
     4321 ccttatcttt ttctatatac tttctatatt catcaaaagt agttgcacca atacagttca
     4381 attcacctct tgcaagcata ggtttaataa gattacctgc atccattgaa ccttctgttt
     4441 taccagctcc aacaatagta tgaatctcat ctataaacag tataattttt ccatcagaac
     4501 tttgaacttc ttttaaaact gcctttaatc tttcttcaaa ttcacctcta tattttgcac
     4561 cagcaattaa agctcccata tcaagtgaga atacaatctt atcttttaaa ccttctggga
     4621 cgtctcctct tactattctc tctgctaacc cttctacaat tgcagtttta cctacacctg
     4681 gctcaccaat caatacagga ttattttttg ttcttcttga taaaattctt ataatccttc
     4741 ttatttcttc atctctccca attacagggt ctaatttatt tttcttggct aaatctacta
     4801 agtttgttcc atatctagca agtgcatcat atgtgccttc tgggtcttgt gtttctactc
     4861 tttgactacc tctcacttgt gataaaatat ttaaaaagtc attcttattt atattatatt
     4921 gttttaatat tttaccaaca gctgattttg attctgtttc catcattgca agcataacat
     4981 gctctacact tatatatgaa tctttaaatt ctttagaaat actctctgcc tttataagca
     5041 cctcatttat ttttctagta gctgatactc cttgtgagtc agcaccttct ccatatactt
     5101 ttggaagctt atcaatttca aagtttacag tatttctcaa agaatcaatt gatatattca
     5161 ttttttcgat tatatttggt atcaatccat cctcttgatt aataagtgct gagaaaagat
     5221 gaattacatc tacttgttgg ttatgatttt ttactgcttc gttataagcc tcatttaaac
     5281 ttttttgaac tcttaaagtc attttttcta cgtccatata aatccctcca ttttaatatt
     5341 atatccaaat aaattaaatt ctctcattta taattattga ttttctaagc tctaaaagac
     5401 tatcaagtaa attctcgttt ttttgtatat cgaaactcaa tgttaaatta aatgttccat
     5461 ctgaattaaa aaaatcgaat acttcttttg attttgctat taaatacaag tcttcatcta
     5521 taagtctttt ttcacctatt aataaatact gacatttatc tataaaactc tttttaaaat
     5581 caatagtgta tagattttct aaaatattat attgataaat agcaatatta ttaaacactt
     5641 ttaatatgtt tgaatttaca aaattgtaaa tttgattatt gttatacagt gatatttgtt
     5701 cgtatatata ttccctaaat aaaggatgaa tgttttgctt aagttttaaa gtaacttcag
     5761 ctggtatttc aaaataaagc ttccagtact tagagttatt tttttctaaa ataaaagcta
     5821 ctttgttcaa actggtagaa ctaatctcct ttagtgtaca attaaaatta tagtgtctca
     5881 aactagtttt ttcattctca aatacatagt caaaccactg tagaacattt ctatcaagtt
     5941 ttaaactcat ataaaaacct tcttccttat ttactaacct tattaggtct ttttatctga
     6001 ttatattctg ctaataaact ttctttatca gctctactac ctttatatgc tatatgtgga
     6061 tttatcatat actcaccatt tttcactctt tgtaaaaaat ccattttttt cagctccgta
     6121 aacaatgaag ataaagaagt ttttggatat tcaaattcac aagataattc atataatgtt
     6181 tttctaatct tattattttt atccatagag tttattaatt ttaatggaat taaatccctc
     6241 ttagtataca ttccttctac tactttaata taattcttaa aacaaacatt catatactta
     6301 cttttattca taactaaact tttgctctct gtatattcct gatttaaatc cttttttaat
     6361 gtcttatcca aattaaata
//
LOCUS       DAELNY010000197         6303 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.200, whole genome shotgun sequence.
ACCESSION   DAELNY010000197 DAELNY010000000
VERSION     DAELNY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.200"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(416..823)
                     /locus_tag="KPE55_003222"
     CDS             complement(416..823)
                     /locus_tag="KPE55_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494263.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1297..2181)
                     /locus_tag="KPE55_003223"
     CDS             complement(1297..2181)
                     /locus_tag="KPE55_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0494264.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2472..2948)
                     /locus_tag="KPE55_003224"
     CDS             complement(2472..2948)
                     /locus_tag="KPE55_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG0494265.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3214..4389
                     /locus_tag="KPE55_003225"
     CDS             3214..4389
                     /locus_tag="KPE55_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG0494266.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4470..5273)
                     /gene="proC"
                     /locus_tag="KPE55_003226"
     CDS             complement(4470..5273)
                     /gene="proC"
                     /locus_tag="KPE55_003226"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0494267.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5313..5897)
                     /locus_tag="KPE55_003227"
     CDS             complement(5313..5897)
                     /locus_tag="KPE55_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494268.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2071 a         1090 c          712 g         2430 t
ORIGIN      
        1 gctactacct atgcagttct cttatgaact tcttacactt tcatgtaagc acagactata
       61 tcttatcctt cagcattatc cgttaaggtc tacccacttc cactaccaat agcttgtagt
      121 gtacttccct caagaggaat agtcgttgaa gtttctccta ttcggagttt acctgctgat
      181 tgcccatttt aaagtactta ggatttaacc ttataccatc taaccaattt tttctacttt
      241 cgtaactttc acacttaggt ttatttcatc cttatgtttt agtttgatta gctttagggg
      301 tttccagcaa ttcaagtagt attggatagg ttctatccta tctctacata taggtttccc
      361 tatatgctga ctattttagc tactaactca tgactaaagt cacgagtgtg cgtagctatt
      421 ttttaatcaa tattttagta aattttacgg taagtaaaaa cctctctcct tcaaatccta
      481 tagcaaatat acttacaagt tgctttgaga aaataactcc taatatagct acaactatgc
      541 atataccaac tattatattt aaaacattgt ttaaaaactt tagtgcttgc ttttcacctt
      601 gtttactact tatatcttga tacattggaa taaaagtagt aactatagca gtacctattg
      661 ctgcaaatat aatatttggt atattcatag ctgttaaata actctctgta tataagcctg
      721 taccataaat tgatgataat actaattctc tacccattcc aagaatctta gagattatag
      781 taactatcat caaataaaaa gttgctttag ctaccttgct cattttatca ctccacttct
      841 aacatacctc tttttaatca agtaaaattt agctcattag atataattct atcaactaat
      901 ttgccttgct tagccctatc aaaatataat taacacctat ttcttaatat atcacaaact
      961 acattattct tcatattttt ttatttctcc aataagtttt acatttttcc tatataaatt
     1021 gtatagcttc aattatattc aaagaaatgg agttgctacc cgataacttt aaaagtaaaa
     1081 aacagatttt attatctttt taaactatat tctaacatat tttgtccata ttgagaaatt
     1141 ttactaaact attatgtact ttattaaata aataaaataa cagcacacct ataaaataca
     1201 agtgtgctgt tcataaacag attttataaa aaaataattc ctaagtagag attaacatgc
     1261 tttctcatac tctcatatat aatgtgtcta aaaatattaa taaaaatgct ttaattgttt
     1321 taacttactt ggatgtctta attttcttat tgctttagct tctatctgtc ttattcgttc
     1381 tctggtaact ccaaatacct ttccaatctg ttctagtgtt ttagggtcat catcatcaat
     1441 tccaaatctc attcttaaaa cttgctgttc ttgctctcca agacctgtaa gtagttcttc
     1501 tatcttttct tttaaattat gctgttcaac ttcgtgtatt acaacatctt taaaatcagc
     1561 atctgatggt ataaattcaa ctaaactact atcctcatct tcttttaatg gagtatccaa
     1621 agatacaaca tttttatctc ttcttaataa ttccaacact ttgtcaactt ctaagccaca
     1681 tgcatctgca atctcatcta tagttggctc tcttaataat acatttaaaa gttcttgttt
     1741 ctttttcttt acaaaattta ttctctgatg aagatgtata ggtatcctaa tagtattttc
     1801 gcaatcgtcg atatatcttg ttatactttg ttttatccac caagttgcat aagtgctaaa
     1861 tttgtatcct tttttataat cgtacttttc tacagctttt ataagtccta tatttcctgc
     1921 ttgaattaaa tccaacatat caatactatc tcttttatat ctctttgcta tgctcactac
     1981 caacctataa tttgaattta taaatttttc ttttgctact gaagatgagt tgattatttc
     2041 cttagccagc tctacctctt ctcctgctga caacattttg tattcctcta tttcctttaa
     2101 atacattttc atcgcattcg atacatttga tgaacccaca caaatataat tttcataatt
     2161 attttctttt ttagtatcca taatcaaacc ccttctattc atagaatttg ctaaaacaaa
     2221 aataaataat ttaaacctac ataatctaca attacataat atcccattct ttaaatgaat
     2281 tgttttgtat attatgtata caaatttata aaaactatac ctataatatt attctacttc
     2341 taacagcaat atcctcctta tatatataat ttattttatt atatgttgtt atattcttag
     2401 ttttttgaat gatatatgtc taaaaaaaga ctgtttacta aacagtcttt ttttatatat
     2461 atgctcaata tttaattttc atgatgatga ttacatccac atccacaatt ttcatcatgg
     2521 tgatgatgtt cttctaaatc ttttacctgt tgttccattt catgaaactc ttttaccatt
     2581 atatcataag ttggaagagc tgcctcagta tcgtattctt cccctctcca atcagcgtac
     2641 atcatagcat ctccattagt taaaaccaat ctacctgaca tagttatatt atccacttct
     2701 tctaaaaatt ctatttgttt ttgtaaaact tctccttcta actccacatc ttcatctaaa
     2761 cattgcacta tcattacagg tgcataataa tcttcttcat ctttaacatt tacaacaata
     2821 tctaaaattc cttctgcacc ttcttcaaat tctgccaact caacatttgt atcaatcata
     2881 tcttgaggta ctaataaatc ttctattaaa tattttatta ctctatcttt tactaattct
     2941 tttgacattt atttttctcc ttccgattct ataacattat cataatcatt tctatattat
     3001 agtatactac attaattgtt ttttcaatct cttacaaaaa ttatttttta ttatcttcct
     3061 tatattccct tttttattac aaagttgtaa tcgcattgat ttaaaaaata gtttatattt
     3121 agtataatag aaatgtagca catattttta ttcaatatat taataaaaaa atatattgaa
     3181 aatgtaatgt atgttaaatc taggaggaat attatgaagt ttaaaaaaca aattagttct
     3241 attgttgtgt ctactatgct tgtacttggg agtatgaacc tttcttatgc agacggtaca
     3301 aatgtagtta cacttggagc taacttatca gagtctcaaa agcagcaaat gcttaattac
     3361 tttggagtaa aaaaagacca agtcttagtt ttagatgtaa caaatgcaga agaaagacag
     3421 tatttacaag gtgttgcaac agagggtcaa cttggaaaag ttactatctc ttgctcatac
     3481 gttgaaccta ctaaaaaagg aaatggaatt aatgttaaaa cagcaaactt aacttgggta
     3541 actagttcaa tgattgcttc aacccttaca acagctggtc ttgaagatgc aaatgttatt
     3601 gctgctgctc catatcctgt aagtggtact ggagcattga caggaatcat gaaagcattt
     3661 gaggatgctt caggaaagaa acttgatgaa actaagaaag aaattgcttc tgaagaattg
     3721 gtagttacag gtgacttagg agatgatata gggcaagaaa aagcaacagg tgtaatgaat
     3781 gatataaaaa cagaaattgt aaagaatggt acaaaagata ccaatcaaat agcaaataca
     3841 ataaataatg ttgtaaataa ttacaatatt actattacac cagaacaaaa agaacaaatt
     3901 aaaggtgtaa tgaaaaaaat agctgaccaa aattacgatt acaatagcat gaaaaataca
     3961 ctagataatg tatcagataa tgtaaatgaa caattaaaga aattaggtga aagtgttaag
     4021 ggttctggta tactagatac tattggtgga tggtttagtg gaataggaga ctggttctct
     4081 ggtttattct caggcggaga taaaaaagat ttgggaatct taaataatac taatgatgag
     4141 tctcttggct ctgatgctgt aataaattct acagaaggtg ttcaaataaa tccacaaaat
     4201 aatactaatt ctaacaatga tagtgataca aatagctcaa aaaatgatac ttcaagtaat
     4261 aatgaaaata acgatagttc tcaaacaaat gaaaacaaca caacaaataa ttcagaaaat
     4321 caatcaaata ataatgactc agttggtttc tttgaaaaaa taaaaaattg gctttcaggc
     4381 ttattttaga aattaaacaa ataaaaagaa ttgcctaaaa tggaaatttt ctataaaagg
     4441 caattctctt tatttacaat aaatttttat tactcactca tttttttaga tttttctata
     4501 catgcacata ttgcttttat cacagaagct ctaaaacctt cttcttcaag tactttaact
     4561 gcttctatag tagttccacc aggtgaacat accatatctt ttaattcacc aggatgtttt
     4621 cctgtctcaa gtaccatttt tgcagaaccc ataacagcct gtgatgcaaa cttatatgct
     4681 tgtggtcttg gcattcctgc aattactgct gcatctgcta tagcttctat aaataaaaat
     4741 acatatgctg gtgcagaacc acttgcacca attacagctt ctataagata ctctggtact
     4801 acctcagtat ttccaaaaga attaaatact tctgtaactg cttctaagtc ttctttgttt
     4861 atatttttat ttatcgaaag agaagacatg gcttcattta caagagctgg agtatttggc
     4921 atagttctaa ctattttttt atcttctcct ataatagact ctatatcttt tatagatttt
     4981 cctgctgcta ttgtaactat aattttattg tcatctataa agtcttttat ctcttccaaa
     5041 atatcatcat atatatctgg tttaacagct acaattacta tatcagaggt tctagttact
     5101 tccttcgagt cttttgtagt atttattcca aaatttgcat ctattctatc gagagttcct
     5161 tgactatatg ctgatgctat aaccatatta ggttcaacta attttgaatt tactattccc
     5221 cctatcatag cgcttgccat atttcctgca cctatgaatc caattttttt cactttagta
     5281 cctccttatt gactaatagt aatcgttaaa atttaagctt ctttaactgc atcagatttt
     5341 tcaaatggat ttggtatact tactaccaaa attttcacta ccttattagt atagttgacc
     5401 caattatgat tgttcttttc actattatag tgtattgtgt ctcctggata catttctatt
     5461 tgttcatctc ccaaaaacac tgtaagagta ccctctaata catacacaaa ttcctcacct
     5521 tcatgtacgt aacaacaaat ttcctcacta ctcttacttg gaagaatttc aattaatctt
     5581 ggtagcattg ttttttcctt taaactagat gataaatgat aatgtataaa tgttgagttt
     5641 tctacatcaa atacttcttt ttcataactt cttaatacag ctcttttatg ctcttttggc
     5701 ttcataaaaa agtaagttaa ttctacatct agtacacttg ctatttttcc taaggaatct
     5761 gttgccacac ttgttaatcc tctttcaagt tgtgataaaa aaccaattga caagtttgtt
     5821 ttttcactca tatccttcag cgtcatttgc ttttgagttc tgagttgctt tatttttgct
     5881 cctatgtcct tattcaaaac catcacccct aaataatttt aattttttca taatataaat
     5941 acaaaaatat aaatataggt actgaaccca tctttatttc atttaaaaaa atttttataa
     6001 gtattactat aatttatcaa tctttttttg tctacatttt tacgattatt tacgatttat
     6061 ttttcacata tgtatattta atcttgccaa ctaaagatac attttttata agcatttact
     6121 ctttatataa taaaacttaa gttgttcatg tagtcttctt ctggtttaat tattaaaatt
     6181 tcaaattata ttattatttc tacacattat tacatccttt agtacaatta taacataatt
     6241 ttacaattta atgatatcta ttcaatgtct ttttttactc tacaacatat tttattttag
     6301 agt
//
LOCUS       DAELNY010000198         6300 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.201, whole genome shotgun sequence.
ACCESSION   DAELNY010000198 DAELNY010000000
VERSION     DAELNY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.201"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            246..1190
                     /locus_tag="KPE55_003228"
     CDS             246..1190
                     /locus_tag="KPE55_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG0494269.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1212..1838
                     /locus_tag="KPE55_003229"
     CDS             1212..1838
                     /locus_tag="KPE55_003229"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG0494270.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1872..2921
                     /gene="hisC"
                     /locus_tag="KPE55_003230"
     CDS             1872..2921
                     /gene="hisC"
                     /locus_tag="KPE55_003230"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0494271.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2924..3502
                     /gene="hisB"
                     /locus_tag="KPE55_003231"
     CDS             2924..3502
                     /gene="hisB"
                     /locus_tag="KPE55_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG0494272.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3504..4109
                     /gene="hisH"
                     /locus_tag="KPE55_003232"
     CDS             3504..4109
                     /gene="hisH"
                     /locus_tag="KPE55_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG0494273.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4106..4828
                     /gene="hisA"
                     /locus_tag="KPE55_003233"
     CDS             4106..4828
                     /gene="hisA"
                     /locus_tag="KPE55_003233"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG0494274.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4810..5568
                     /gene="hisF"
                     /locus_tag="KPE55_003234"
     CDS             4810..5568
                     /gene="hisF"
                     /locus_tag="KPE55_003234"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG0494275.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5596..6264
                     /locus_tag="KPE55_003235"
     CDS             5596..6264
                     /locus_tag="KPE55_003235"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG0494276.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2573 a          536 c         1248 g         1943 t
ORIGIN      
        1 gtgagattaa atatagtaaa attaaataca aaattaaatc atcaccatca tcatagatta
       61 aagggattgt aatatgaaat taattcatag acacaagccc tgtttttagt gtaattggtc
      121 ttgtatctat gatgggaagt aattttaaga gaaaattata gccatatagg tataaagcct
      181 atgtggcttt tttattgttt taaatttgca aatataagag taaatttaat gaagagggtg
      241 agaatgtgaa atttttgagg attgaagatg aggttattta ttctaaaaaa agatataaat
      301 taaaaaggga aatagataaa ctttttttgg atgaagaata ttttcaaata gaaccacaat
      361 tatttgaaga atatgatgaa tttacaacta taaataataa aattccagaa gaatctatgg
      421 taaaggttgt aaatggaaaa gtaatggtac ttagagctga tataactaca agtataataa
      481 aaagccttgt accaagatgg gaagatggtt taaagcttaa gcttttttat aattcatcaa
      541 tatataaaaa taaaaataca gttggaataa aagagataag acaaataggt tgtgagtatc
      601 taggagaagc ctctgtagaa gcagatagag aagtagtgaa attagctctt aagatacttg
      661 aaaaatacaa taataatttt attctagagg ttgggagtag caagtatatt catggtttgt
      721 tagaagaatt aaatttaaat aagaattgtg aaaatcaaat taaaaattta ctatatacga
      781 agaacactca tgaactaaaa gtatatattg aagaattaaa aattaagcgt gaagtaaaag
      841 agttattatc aaatatttta agtttacaag ggaatttata taatgttatt gaaaaatcaa
      901 acaagttcta ttgtaataat aagatgaagc aagctcttga agaattaaag caagtaaata
      961 atttgattga ggaatgtaat tttttagata aggctagatt tgatttatca atgattacta
     1021 tgcttgacta ttatgaagga attatgttta gaggatacta cccaaactca tataaagaaa
     1081 ttcttagtgg tggaagatat gattctttaa ctaaagagta tggaaaagaa attccagcca
     1141 ttggatttac actaagtgtt gatgagttaa tgaagtatgt atataagtaa agtttagaag
     1201 gagggattgc tttggattat ttgactatag cactagcaaa aggacgaatt gaaggggaat
     1261 catttaaaaa atttaaaaaa atgggtctag gagacagtat agacacagat acgagaaaac
     1321 taatttttaa agatgaagaa aataaaatta tatatataca tgtaaagcca tcagatgtag
     1381 ttacatatgt tgaaaaagga gtcgctgacc tcggtatagc aggtaaagat actattttag
     1441 agaatgagac agatgtatat gaaatatatg atttaggttt tggaaaatgt aaatttgctg
     1501 tagcaggatt aaagggagat agcatatata gagaagatga atatttaaaa gtggcaacaa
     1561 agtatcccaa tatagcaaaa aagtatttta aagagaaagg tcaaaaaatt gaaataataa
     1621 aattaaatgg ttctgtagaa ctagctccaa tagtagggtt gtcagatgta attgtagata
     1681 tagttgagac tggcaataca cttaaagcaa atggtcttga aatattagaa gacatatgta
     1741 atataagtgc tagaattata tcaaatagag ctagctatag atttaaatat gaacaaattc
     1801 agaatataat tcgtttattt gaagaacttg ataattaaaa tataattgag ctttataagg
     1861 agggatttaa gttgagagaa aaagaaagca taagagagtt aaagggatat gaaccaaatc
     1921 acgtaaactg taaagtaaaa cttgatgcaa atgaaggaag taagaggttg tttaaatacc
     1981 ttattaaaga aatttcagat agtgatatag atttaaattt atatcctgaa gattcttata
     2041 gtgatttgaa agaatctata atagattaca taaatatttc tggagtaaat aagaaaaatc
     2101 ttctagtagg aaacggttca agtgaaataa ttgatttaat aatacatact tttgtagata
     2161 aagatgaagt aatattgagt ttctctccaa gttttagtat gtattctata tatagtcaga
     2221 taaatgggtc taaatttata ggtgttgaaa gtgatgaaaa tttagttata aatatagata
     2281 atgtgattga aaaagtaaag gagaataatc ctaaaatagt aattgtatgt aatcctaata
     2341 atccaactgg aacaatatta aagagggaag agataataaa gcttttagat tcaactaata
     2401 gtctagtggt tttagatgaa gcatatatgg attttggaga agaaagcatg ttaagtgatg
     2461 tttttaagta tgataatcta atagttctta gaactttatc aaaggcattt ggattggctg
     2521 gaataagaac aggatatatg ttatctaata gtagtttaat aaattctgtt gagaaagtaa
     2581 gacctccata taatttaaat tcattatcag actttattgc tacaagagca cttagaaata
     2641 aagatgtggt aaaggcttat ataaaggaag taaaagagga aagagaagtt ttatataaag
     2701 aaatgattgg tatgggaatt aaagcatata aatcacaagc aaattttata ttattttatt
     2761 ctgaaatcga aaatttatca caaaaactta ttgatagagg tgttttaata agaaaatttg
     2821 gtggaaagtt agaaaattat tacagagtta caattggtga taaagaagaa aattctatgt
     2881 ttgttggagc tataagagat attttgaaaa aggagaaata ggtatgagaa tttggaaggt
     2941 agaaagaaat actcttgaaa cacagatttt agtagaatta aatattgatg gttctggtaa
     3001 agctgaaata gatactggaa taggattttt agaccatatg cttaccttaa tgtcatttca
     3061 tggcaagttc gatttaaaag tgatttgtaa gggtgataca tatgtagatg accaccatag
     3121 tgtagaagat ataggtatag ctataggtga agcatttaaa aatgctctag gtgacaaaaa
     3181 aggaatacga agatattcta atatctatat acctatggat gaatctctat caatggtagc
     3241 aatagatata agcaatagac cttatcttgt atttaatgct aaatttgata cccaaatgat
     3301 tggtagtatg agtactcaat gctttaaaga attttttaga gcttttgtaa atgaatcgag
     3361 agtaacatta catatcaatc tcttatatgg ggaaaatgac catcataaga tagaaagtat
     3421 ttttaaagca tttgcaagag ctttaaaaga gggtagtgaa atagtttcta atgagatagc
     3481 atcttcaaag ggggttttgt agaatgaata taatagtaga ctatggtctt ggaaatatag
     3541 attctgtgtc taggggtttt agaaaagctg gaatagaaac taaaatttct agtgatatag
     3601 atgaaataaa acaggcagat tcacttatac ttccaggtgt tggagctttt agagattcta
     3661 taagtgcctt agataaacta ggattgatac caataataaa ggagcatgtt tctaagggta
     3721 aatttatgat aggtatatgt ctaggaatgc aattacttta tgaaaaaagt tatgagtatg
     3781 gagaatatga aggattagga cttataaaag gaagtattga taaactggat ataagtttaa
     3841 aagtgccaca tatgggatgg aataatctaa aatttaataa ggcaaatgat gatatactta
     3901 aatatataaa tgaagatgat tatgtatact tcgttcactc atattatgca aattcatcaa
     3961 atgaagaact aattgcattt tcagaatatg aaaagaaaat ccctgctatt gttagaaaag
     4021 gcaatgtata tggtatacaa tttcatcctg aaaaaagtgg agaagtaggg ttaaatatat
     4081 taagagcata tggggagatg ataaaatgat aatatttcca gcaatagata taaaagataa
     4141 taagtgtgta agactcacac aaggagaatt tgacaaagta aatgtatatt atgataatcc
     4201 attagaagta gcatataaat ggaaaaatga aggagcagaa tatatacata tagtagattt
     4261 aaatggtgct agaagtgaat ttggtgtaaa tacaaaaata attgaggaca tagcaaacaa
     4321 tatagatata cctattcaag ttggcggtgg agttagggat aaggaaaaag tcaaaagttt
     4381 aataaatgct ggtgttacta gagtaatatt aggcagtata gcgattgaaa atttgaattt
     4441 agttgaagaa ttggtcaatg aatataaaga gaaaattgta gtttctatag atgctaaaga
     4501 tggaaaagta gcagttaggg gatgggaagt tgtaagcaat gtagactcat taacattatg
     4561 taaacaactt gaaaagatag gtgtacaaac tattgtttat acagatatat caaaagatgg
     4621 gatgttgcaa ggtccaaatt ttgacatata tgaaagaata gcaaaggaaa catcactaaa
     4681 tgttattgct tctggaggcg taacttctat agaagacgta aaaagactaa aagctatgaa
     4741 cttatatggt gcaataatag gtaaagcgct ttatgataaa aagatagact ttaaggaggc
     4801 acaacaatta tgcttactag gagaataata ccttgtctag atgtgagaaa tggaagagtt
     4861 gtaaagggga aaaaatttaa agatatagta gatgttgata gtccagaagt acttggaaaa
     4921 ttttatagtg attgtggtgc agatgaactt gtattctatg atataactgc atcaaatgaa
     4981 gagagaaaga catctttaga atttgtgaca aaagttgcag aaaatataaa tataccattt
     5041 tgtgttggtg gaggagtaaa taaattagaa gatttcactg atattttaag aaaaggtgct
     5101 gataaagttt ctataaattc atcagcagtc aaaaatcctg aattaataag ggaagcttca
     5161 ttaaaatttg gagcacaatg tgttgtttta tcgatagatg caaagaaaaa tgaagagggt
     5221 tcatggagcg tatacgtaaa aggtggaaga gaaaaaacta atctggatgc aattgaatgg
     5281 gctgttaaag gtgtagaact tggagcagga gaaatcgttg taaatagtat ggatgaagat
     5341 ggaatgaaaa atggatatga tatagaatta ttatcaaaga taacttcttt agtaaatgta
     5401 cctgttatag catctggagg cgctggaaaa aaggaagatt tttatgaggc ggttaataaa
     5461 tccaatgtag atggaatttt agcagcatct gtttttcatt ttggcgagat aaaaataaac
     5521 gatttaaaga aatatttaaa agacatgggt gtagaagtaa gattataagt taaatactaa
     5581 aaggagatta gaaatatgga taataagtgt aacaatgtat acagtgatga agtagagaaa
     5641 tttataagaa gtataaagtt tgatgataaa gggttagtcc ctgttgttgt tcaagaggta
     5701 gttagcaaag atgtactgat gttagcatat atgaataaag atgctataaa gaaaacctta
     5761 aaagataaag tagcttgcta ttttagtaga agtagacaag aactttgggt taaaggtgag
     5821 acatctggta atactcaaaa agtagttaag atgtcttatg attgtgatgt agatactata
     5881 ctactttttg ttgaacaaac tggtgtagct tgtcatacag gcaattattc ttgtttttac
     5941 agagacttgt ttgatgatag ttcaaaaatg gagcttgaag tacaaacaaa tatattgaaa
     6001 gaattatatg atttaatcaa tgaaagaaaa aataatccag ttgaaggttc ttatactaat
     6061 tatctttttg aaaaaggaat agataagatt cttaaaaaag taggtgaaga gagtagtgag
     6121 gtaattatag catcaaaaaa taccgataaa agtgaactta tttacgaaat tagcgattta
     6181 gtatatcata cattggtttt aatgatagaa aaaggtgttg aaattgatga aataaaaaaa
     6241 gaattactta aaagaagaaa atagaataag atagattaat gggtaataag aaaatatacc
//
LOCUS       DAELNY010000199         6204 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.202, whole genome shotgun sequence.
ACCESSION   DAELNY010000199 DAELNY010000000
VERSION     DAELNY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.202"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..1837
                     /locus_tag="KPE55_003236"
     CDS             56..1837
                     /locus_tag="KPE55_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG0494277.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1919..2449)
                     /gene="lepB"
                     /locus_tag="KPE55_003237"
     CDS             complement(1919..2449)
                     /gene="lepB"
                     /locus_tag="KPE55_003237"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0494278.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2489..3313)
                     /locus_tag="KPE55_003238"
     CDS             complement(2489..3313)
                     /locus_tag="KPE55_003238"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0494279.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3570..4478
                     /locus_tag="KPE55_003239"
     CDS             3570..4478
                     /locus_tag="KPE55_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0494280.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4622..6067
                     /locus_tag="KPE55_003240"
     CDS             4622..6067
                     /locus_tag="KPE55_003240"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG0494281.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
BASE COUNT         2521 a          771 c          946 g         1966 t
ORIGIN      
        1 ataaaaatat agttcaaaat ggtataatta agtataacag aaggaggtaa tgtaagtgag
       61 ccaatacata aatataaatt ctcagttaga cgccctaaaa ttgaaggagt tcgagaaaat
      121 attttacgaa aatatcaatg aggacgttaa agtagtgcca aaagttactc cttttaaagg
      181 aataaatacc gatttgttgt atgttaaaga tggaagaatt ttatttataa aatttatgga
      241 tacaacagaa gatatatttt ttattttaga agaagagtta cttgaagtta tgaatgagga
      301 atatgaactt ttgaaactaa aaatgggaca aaagaataga aatataagtt ataactacgt
      361 ttacattatg ccatacgttg aagttgagga aacatatgaa tttgaagaat ttgttaataa
      421 taatataata gataaaaata agcttcaaga tataatgaat aagggttcgc tggaggaata
      481 tcttaatgat gagaatgatg aaataaactt aaatttattt ttactagaca tatgttcaga
      541 atactatata ataaatgata aacttcatct aaatgaaaaa tttaaaaaaa tttcattcta
      601 caatgatgac tataagtata cagcaactat gatggaagac gttcaaatta aagatgttgt
      661 ttctattaaa tatgggaata cacttattga aggtggttct ggtataggca aaacagcaat
      721 tatgctttct agggctataa aattagcaaa agtatatcca catcataaat tagtcatatt
      781 tacacataca aaacaactgt gcaatgagct tcgagagagg atagagttat tatacaaaga
      841 taataacaat ttagaagttc acacatttag ttcttttata tttaaactag ctaaaaaatt
      901 caatttaatt gtagattata acatgttaaa aaatgattac gaaaagacct ttaataatct
      961 tgtaaaacaa gcacagaaca ttataaaaaa taaaaatatg ttcaaggcta tttttattga
     1021 tgaagcagaa agctttttag agtatgaaat agattttata agggagtttt tatataaaac
     1081 aaaattcata tttaatgtgt gttcttgtaa ctccttgaat atctctaata gattgaatat
     1141 atttaaaaaa ttatataatg gtatagaatt tgatgataag attatcttaa gtaaaaatta
     1201 tagacaagct aaagagattg tagattttac aaataaattt tcaaataatt ctaactcata
     1261 tatcaacgaa ttaagaccaa atacagaatt tagtactttc ttttatacta aagcacttag
     1321 aggtggaaat aagtctgttg atataataaa agtaagtgac cttgatgacc aaataagttc
     1381 tgtaatctgg gaaattgagt atttaataag taaaaaaggt cttgattatt ctgaagttgc
     1441 tatcgtttat ccttacaata agaagaaatt aaaaagtgga aaaaccattt actttcaata
     1501 tatgttaaaa aaagcattgg aagaggcaaa aattccttat atatgtgcag aagataattt
     1561 gacaaatata agtaagaagg taggaactac aatagcaaat atatatgcaa taaagaattt
     1621 agagtataaa gcggtaatag tatgtgaatt agagatgtta tacaatcaaa ctataaatga
     1681 tattgaacaa gattatcaag taaatgactt tgtaggagat ttaaataagg tttatttagc
     1741 tatgagtaga gcaacagact acctaagtat agtaacaact tttaatgaag aagcaagtga
     1801 tataataaga cttattacag aatcaaaaga tatttagtaa aaaaatattt gtaatttaag
     1861 aaaataaaaa gagactgaga tttgtaaaat aaactttacc aattcagtct cttatttttt
     1921 agtctacttt cccaatatta tcaagaggta atagtctcac cataacctta ccaagaactt
     1981 catcttcatc aaccatacct acatcaggga atctactatc attactattt tctctattat
     2041 ctcccattgc aaatagttta ccttttggaa caacagtatc tatatctcca gaagtatagt
     2101 tattgtgtat atatggttca tttaataatt ttccatttac atacacttta gaatttgata
     2161 tttttattct gtcaccttca gtagctataa ctctttttat taagtctttt ttctttcctg
     2221 tttctccatc aactaaattg gttttaaaaa ctacaatatc gcctttttca ggtttaccaa
     2281 ccttgtatga tatcctattt aaaatcagat aatctttatc atctaaagta ggatacattg
     2341 attctccact tacaatagaa ggtcttataa attgtagaat tacaaatgca agtacaatag
     2401 ccatagctat tgacttaatc caatcaaata tttctttttt aacactcata acttttctcc
     2461 ttatcatttg ttattaaatt agaatacatt ataatagaaa gaaataagca agaaccacta
     2521 tacccaagac tattctataa taaccaaata ctttgaagtc atgttttttg atatagtcca
     2581 taaaaaattt aataactaca attgaaacaa caaatgctac tacagaacca acacctaaaa
     2641 taagccattc aaatccagta aatccaaatc cagcttttag aagttttaat gcactagctc
     2701 caagcattgt agggatggct aagaagaatg aaaactctgc tgctacatat ctagaagtac
     2761 caagtaatac tgcacctatt atagtagaac cagacctaga tgtacctggt ataagagcta
     2821 gacactggaa taatcctata caaagagcta atttatatgt aacttgagaa aaatctttat
     2881 acttaggttt tttatttctc ttttcaagcc ctatcattat tataccatat acaataagag
     2941 caatagcaac tactgttgaa ttaaagaata atctatctat atcatcttca aacagtattc
     3001 caataactcc agatggtata actgcaatta taaccttaag ccataaacgg actgtttctt
     3061 ttttctgctt aatattcttt gcgctatcaa aaggatttaa ttttctaaaa aatataacaa
     3121 gtacagctaa tattgaacca aattgaatta caactaaaaa tgtacttata aatgtatttg
     3181 aaaaattcaa ttttataaac tcatcaacta atatcatatg ccctgtacta cttactggta
     3241 gccattctgt tattccctga actatgccca aaatgactgc cttaaaaatt tcaagcaaac
     3301 taaatgtttc caaatcaaaa tacctccaat acaaatttat agtagcatat tttattcata
     3361 gattaataaa aaaatgccac cttttactaa cttataatac catagtactg tgtagtgcac
     3421 aatggaagat tgatattttc ataaaacctt cataatataa caaaataaac ttaaaagtta
     3481 tctattgaat aaggggtata tttgaggggg aaatatatgg tatagtaaaa gtatagctat
     3541 tttattttat gaaaaggaaa ggagttatta tgtatagtac aaaaatacta gatgattatg
     3601 agtatgaaac ttttaatgtt ttttctacaa aattaaatcc aaacacgaaa cacgattatt
     3661 tatcaaaggt tatactgttt aaagaatttt taaaagggaa agaattgatt tatgctacga
     3721 aagaagattg taaaaatttt gtagactata tacagactaa atatgcgaaa tcaacttgtg
     3781 aaaagatata tagttattta catagttttt ataacttctt aaaaaaagaa ggatacatag
     3841 atataaatcc atttagatat gtagaaaaac ccacagtaac aagaataaaa acaaaagatg
     3901 atgttctaag tatacaagaa ataaataaac ttataggtat cctaccaaaa ttaaatataa
     3961 gagatagagt aataatagtc tgtctagtta ctactgggtg tttattaaat gaattagtta
     4021 gcttaaagtg gaaagacctt atggtagatg aaaacgataa ctcatatgta aggttaggta
     4081 agggaagaaa agaaagagta gttaaattac atccatactt ttttaaactt ttagaagatt
     4141 acaggaatta ttctgggctt ccagaggtaa taatacctag tgatgatttt atttttacaa
     4201 ctcaaaagag taattctata acagatagaa atgtcagact tatagtcaaa aaagcattag
     4261 atttagctgg actttctcaa tattcagcaa gagattttag acattcattt gcagcaataa
     4321 gtttaaggct tggggcagat gaaagtgatg taaaaaatca gcttggatgg agtgataaat
     4381 actatgcaat tagatataag tatgtattaa attttgttga tagtgaaata gtggattact
     4441 taattgaaaa agataatttg gatataaata aaaaatagta ataaataaaa aatactatat
     4501 ataaatgtga acaaaaaata aaaaagtctt gttatagttc gtaaaattat atataattat
     4561 ttatgtacta atgtgtacac agtaatcaca cgacaaaaga tttaaggaag gtgctaatat
     4621 aatgatagat aataaatata ctacttactc aaacccactt acagacagat attgtagcaa
     4681 ggacatgagt tatatatttt ctccacaatt taaattctct acatggagaa ggttatgggt
     4741 tgcacttgca gaaggtgaac atgagttagg aatcaatata actgaagaac aattagatga
     4801 gctaagagct aatatagata atataaattt tgatgaagct agaaaaatag aaaaagaagt
     4861 aagacatgat gtaatgtctc acgtaaaagc ttatggttta caatgtgaaa aagccaaagg
     4921 aataatacat ttaggagcaa catctgcata tgttggagat aatacagatg ttatacaaat
     4981 gaatgaagct ttaaaacttc taagagtaaa gcttgtaaat ttaataaaca atttaaaaga
     5041 atttgcactt aagtacaaag atatgccagc tcttggattt acacacttcc aagctgctca
     5101 acttacaaca gtaggaaaaa gagcttgttt atgggctcaa gatttaattc ttgatttgga
     5161 agatttaaac tatagaatag acaacatgaa gttaagagga gttaaaggta ctactggtac
     5221 tcaagcaagt tttttaagct tatttgaagg agaccacgaa aaagtaaaag agttagataa
     5281 gaaaatttgt aaaaagatgg gataccattc ttcctatgca gtaagtggac agacatatac
     5341 tagaaagtta gattatcaag taataagcat actatcaggg atagctcaaa gtatgcataa
     5401 gatgactaat gatataagac ttttacaaag tttaaaagaa ttagaagagc catttgaaaa
     5461 aaatcaaata ggttcatcag caatggctta taagaggaat ccaatgagaa gtgagagaat
     5521 agcatcttta tctaagtata taatctctga atctataagc cctgcaatgg tacaagccac
     5581 tcaatggtta gagagaacat tagatgactc agccaataag agattggcaa tacctcaagc
     5641 atttatggca gcagatgcta tacttgaaat agggataaat gtaactgatg gtttagtagt
     5701 atatgaaaat atgataaata gacgtgtaaa tgaagaactt ccttttatgg ctacagaaac
     5761 aatacttatg gaagctgtaa aaagaggtgg agatagacag gaattgcatg aaataataag
     5821 agaatattct atgaaagctg cctatagagt aaagcatgaa ggcaaggata ataacttaat
     5881 tgaacttata atgaatgatg attcatttaa aatgagcaag gaagaaatac tatctataat
     5941 ggacccaaaa aactttatag gtagagctcc agaacaagtt gtagaatttg taaatgaagt
     6001 tgtagaacct gctataaaag attataagga agacttagga aaagtagatg tagacctaag
     6061 agtatagaat tgtacagata aaatataatt aaaaacggct atcttattta taaataagat
     6121 agccatttta atgggctagc gtcttctctc caccgctgtg aagagttctt ctttacttac
     6181 tataccatga atttaataaa atag
//
LOCUS       DAELNY010000200         6199 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.203, whole genome shotgun sequence.
ACCESSION   DAELNY010000200 DAELNY010000000
VERSION     DAELNY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.203"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(416..1006)
                     /locus_tag="KPE55_003241"
     CDS             complement(416..1006)
                     /locus_tag="KPE55_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG0494282.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1009..1884)
                     /gene="dpsA"
                     /locus_tag="KPE55_003242"
     CDS             complement(1009..1884)
                     /gene="dpsA"
                     /locus_tag="KPE55_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG0494283.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(1960..3351)
                     /locus_tag="KPE55_003243"
     CDS             complement(1960..3351)
                     /locus_tag="KPE55_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG0494284.1"
                     /translation="MNYYELLRIKKERQKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3355..4491)
                     /locus_tag="KPE55_003244"
     CDS             complement(3355..4491)
                     /locus_tag="KPE55_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG0494285.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYDTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4523..5068)
                     /locus_tag="KPE55_003245"
     CDS             complement(4523..5068)
                     /locus_tag="KPE55_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG0494286.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            5392..6171
                     /locus_tag="KPE55_003246"
     CDS             5392..6171
                     /locus_tag="KPE55_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494287.1"
                     /translation="MKVNENNINNIDEELGELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEISLSQREHFKI"
BASE COUNT         2145 a          964 c          750 g         2340 t
ORIGIN      
        1 ggataagcat ggaaaaatta aaaacaatta gcgttttttc tttgattata agtgtaattt
       61 taacgattgg aggtattgga atagtaactt attatgtaga taatttgttt attagaggac
      121 tatcagtatt tgttttaatt atgtcctctt cttttgtatc aacaacagtt agattgatat
      181 ttgaagagtc aaaacgatat aaattctaat ttaaaaattg gattaagtaa ataattttaa
      241 tatagcaaaa attacggaga ttagctcaaa atagactttt tagtccatga ggcatttttt
      301 tgtatgaaat taaatatatt ttcatcattt caacaagaaa aagaggcttc tctctatttt
      361 gagacagcct ctttgctagt attgtatcta atacataata ttataagaac caattctatt
      421 gatgtggact ttttataaca ggctgtagct gtttgttctc tagagcaagt tctatagttg
      481 gaattattag ctcagtatgt gcaatcatag aattaggctt tgctttgcaa ttatcttgac
      541 caaaaggtac aaaatatata tttttagaat taaggagaat gccaatattt ttgaaattaa
      601 agcttagagc atcattcgtt gatattgata ttactagtgg tttatcatta cgaagatgac
      661 cttttgctgc cattaatact ggtgaatctg taataccatt tgctaatttt gcagcagtat
      721 ttccagtaca aggagctatt attataatat cagcaattcc tttaggtcca aatggctctg
      781 cctcttcaat cgtaactata ggtttatttc ctgtaagttc atatgctttt ttcataaact
      841 cttcagaatt tccaaatcta caatcgatat tttgtgatac atctgaaaat atagtgtgta
      901 cattagctcc ttctttaacc aaattctcta actctataaa tattttatca taagtacaaa
      961 acgaaccagt aaatcctact ccaattgtaa gtccttcaag tctcatttct aatcccttct
     1021 ttcatttata atattttcta tagcgttaac aagaatttca ccagatgtct ttggtgaata
     1081 tttaccagga agcccaaggc aaagagtcgc attaatccct gcttctttaa caacagaata
     1141 atctactcct ccaggagaag aagctatatc tataatagtt acttcttgtg atactttttc
     1201 taacctttct ttcgttaaaa tacactctgg tatggtgtta aaaatataat caaattgtaa
     1261 aagtttttca tcaagttgac ttaaagaaat actggaatat ccaaatgcat tagccgtact
     1321 tcttgcatca ttatttcttg cgccaacaca tacatttgca tccaaagcgt ataatttttt
     1381 tgcaagtatt tgtgcacatc ttccataacc taatactaaa cagttacttt gatgtatatt
     1441 gattatactt cttttaatag cttctgctat agccccttca gctgtagcaa ctgcatttaa
     1501 aatagcaaca tcattcattt tcataaaatc ataaatctga atagagtgtt gctcgcaaaa
     1561 tttacaaact ttagttggta tgttacctcc aaataatatt tgattaggag ataaattaat
     1621 tagcaagtta tcaatagtag catcaagaca atcatccgta ttttcaatat taacttgatt
     1681 ctttgtaaat ggaattggac ataaaataat ggaacttttg gatagtgctt cagctagtga
     1741 agttgatttt ccaacgattc catttataat ttcatttgat atcccataag tgactacaga
     1801 atacccttta ttaattagtt tttttaccat gtacacttgt cgcaggtctc ccccaataac
     1861 agttatatcg tattgatttg acatattgta agcctccttt actatataca atatgttttt
     1921 atcaatacat tggttcacta aaattactaa atattatact tataacattt ttaataatgc
     1981 taatctatct atcttttcag aagaggtata tggaaatgaa tttaaaaaga taattttctt
     2041 tgggatttca tttttcatta gattatcttt cattttaatc aacaaatctt tcttcgtaat
     2101 tttgtctata gttattacaa ataatacaat ttgatttcct cttatagtgt ctgagtacgg
     2161 caatacaatt gcatcttcaa tttgaggaat ttttttaact tcattttcaa tcttagtaga
     2221 actaacttta aatcctccaa tatttataat gtcatcactt cttccttcaa atattaagta
     2281 tccatcatta tcataatatc caatatcatt gacagaataa ggtttggttg cattatatac
     2341 tgcatattct gtattcacaa aaatttttcc ttctctaata tatatatcta ttcctggaaa
     2401 tggtctgcct acacttagag gtttttcaat tagttcatca tagcataaat atgtaatata
     2461 atttaattca cttgccccat agtataaaat aatttcagac tttggcatat atttttttag
     2521 atttcttgca gtatcttcaa ataataattg agaccctgta aaaatactta caactttgag
     2581 tactggttct tttaaatgtt taaccaaaag ttgaagtttt gtaggtataa ggtatatgtt
     2641 agttacatta tattgtttta ttgtccttat ccatgattta caattaagac ctgaacttat
     2701 tacaatacta cctccttcat ataatactga cattatagaa tttagattcc ctgtaaaact
     2761 aagtgttcca ttaacaaaca agattgattc cccagagata ttaaaaatac cattttgtat
     2821 aggaaaaaat ccagcccaac tctcatatgt tctatataat acctttggaa cacttgtaga
     2881 acctgaagat aaagctccca tacaaatact tttatctaaa taatttataa gcttcttata
     2941 ttgatacata tatatattaa atttaggatt aaaaatagaa aaattaactt tttttgctac
     3001 ttcattattg ttatcatcat ataaatcaat aacgtccata gattcatcag atataatgat
     3061 tgaaatatta ttttttaata aaatatcatc taatactttt ttagaaagat tataatgaca
     3121 aataatagga atattttttg aataattaat agcaaaaaaa ctaatcaact gaaacattat
     3181 atttttagaa tatattaaaa ttgggatatt ttcacctaaa gaaatttgct ttcctagttc
     3241 ctcactataa ctcaatatat tttcataagt atatttttct ccatctataa ttaaaaaatt
     3301 tttatttttt tgcctctctt tttttattct taaaagttca taatagttca taatctacac
     3361 cctttcaagc agtaaagctg aacctagacc accagctgca gctatagagc aaattccata
     3421 tctaccctta gtcttttcca atgcttttaa aagatgtaat gcaataattg cacctgaagc
     3481 accatatgga tgtccatacg ctaaagctcc accaaatata ttatatctat ctattaactc
     3541 tggatacttt ctttgaaaca acacatctat aactgcaaat gcttcattaa attcaacagc
     3601 agatacatct aaataattaa gactttctat ctctaaaagt ttatccattg cttgtatcgc
     3661 tgatgttgga cttaaggttg catctgttcc tattgtgcat gtattaataa tttttgcttt
     3721 aggcttcttt tttacatgtt ctaaatactt cttagaacat attataataa atgaagctcc
     3781 atcattaata agacatgaat ttgctgcatt tgtaatagtt tcttttccta aaattgatgg
     3841 catcctatct aataaccttt gactcatctt atcacgtata ccttcatcat aggtactatt
     3901 atttatagga gatataatat cctcaagtat tttttcttct cttgcttctt ttgctctttt
     3961 atgactttcc tttacccaaa aatccaagtc tgctttctca atttgatata gctctgctac
     4021 tctttccgcc ccttcgagca tactattttg gctattatca tcaggagaaa attgagcaac
     4081 tgtataattt ggattatttg tatcatatcg cttatcattc ttatgatatg tgcgcattgg
     4141 ttgtagagaa ctactttcaa aaccacctgc aataattaaa tcacactgac cagatttaac
     4201 ctttgaaaaa gctatgtcta tactcatcat agcagaagca cactgcatat ccacagtaaa
     4261 agcaggtact tcatttgaca ctcctgctgt taaagtcatt aatctagcta tattccctcc
     4321 tgtcccaact gcatttccac atataatctc atcaattttg tcaatttcat actttttaat
     4381 taaatctttt aaaacacttg ctcctagtaa ttcaggttga acaaattgaa aaattccatt
     4441 ttttagacct atatgacttc tcaatcctcc taatataaaa acttccttca tacattacac
     4501 ccttccttat attatagatt tatcatagat ttcttctgag tacattgttc agtttagtgc
     4561 ctaaaaaaga tgcaataact gactttattg tatctccaat taaaaatggt actactgctg
     4621 caaaaagtgc tgcttctact gtcatttgat taaaatagca cataaatact gttcccataa
     4681 aatatataat aggcattcca acaaaaattg ttattagtat atatctctta atatcattgt
     4741 ttcgtccttt taataaacta ataattagtg ctgcaaacaa aaacccaaaa taaaaaccac
     4801 ctgttgggct taacaacttt ccaataccag atgtacctcc accaaatact ggaatcccaa
     4861 ttaaacctat cattatatat acaccaactg tactaaatgc ttgtcttggt gttaaaatca
     4921 aagctattaa atttatcatt attgtttgtg ctgttactcc tgctggagta aatggcaatg
     4981 gtatatatat gtaagatgat atacacaata atgctacaca tattgacatt ttagttaaat
     5041 cttgaatatt tactttagta tttttcattt ttataattcc cttcatatgt cttatttcat
     5101 ttagtttata tgtacatata caaagcttct attaaagctt tgttttagta taaaataaag
     5161 acgttttttt gtcaactatt ataattattt aggtttacat ttaatttata agtaaacttt
     5221 atactgttac tttatatttt aatagtcatt tataaaactt tgcttaaaaa cagctctatt
     5281 tagactatac ttgtaaatta taatatgata tatttttata taagatttta ttttatataa
     5341 aaaatagtaa caaatagttt atacacaaga tatttaaaag gaagtgatga tatgaaagtt
     5401 aatgaaaata atataaataa tatagatgaa gagttaggtg aacttagtag gaaagtttac
     5461 ttagagtccc cagatttttg gataagtatt tctgacaaaa taatagataa agtatttgat
     5521 gaaatgttat tattttttgc agttaaatat aactatttca atatagtatg ctttgctatt
     5581 gaaaacaatt atctggattt aaacatgcct tcaaaaaaca aaaaatacac taatataatg
     5641 gagcatttgt tagatacatc aaagcagaat gaagataaaa aaatttataa ttatcttcta
     5701 agcttaaata aaggtaataa cctagatgaa agaccaattt tagatgaatt aaactgtaat
     5761 attagtagtg atacttttga aaaaaaagat atatatcttc caaagtactt atgtccaagt
     5821 tgtaattcta atatatttga tacaggttat aaagtatcaa atgacataac atataaattt
     5881 tcttctaaag aagctgaacc tattgaaatg tgtagtgaag tttcttctat cagatgttgt
     5941 aattgtgatg aattattaga agatattaca cttgataaac tatataacct atgcaaaatt
     6001 caaaactgta ataattgtgg ttctaattta actaaggtag gaattattgc aaagaaaaaa
     6061 atgaactttg atgagaatac tgaaaaattt aaagatgtct caacatctta ctgttgtgga
     6121 aattgtgact ctgaaataag cttatctcaa agagaacatt ttaagattta aatatttata
     6181 ggctaaagta ataagatta
//
LOCUS       DAELNY010000201         6197 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.204, whole genome shotgun sequence.
ACCESSION   DAELNY010000201 DAELNY010000000
VERSION     DAELNY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.204"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..528
                     /locus_tag="KPE55_003247"
     CDS             <1..528
                     /locus_tag="KPE55_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494288.1"
                     /translation="SNRVYTNVNKIVFKQLNISNTLKAPKNLRDILKVNVNSKIFDVK
                     PMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNRSNFKTVNKSNDIKKDKYYKNDIQN
                     NNENINDCKKQISFGDNKVKKITKTFGFSCFICSPVGIIYEDMKNINIKIEHTSINEL
                     NPEELFVNTSLLLYY"
     gene            818..1360
                     /locus_tag="KPE55_003248"
     CDS             818..1360
                     /locus_tag="KPE55_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0494289.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1688..3181
                     /locus_tag="KPE55_003249"
     CDS             1688..3181
                     /locus_tag="KPE55_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0494290.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNVQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEQSRITHGKVLFEEYSKG
                     VLKEVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            3255..3488
                     /locus_tag="KPE55_003250"
     CDS             3255..3488
                     /locus_tag="KPE55_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494291.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            3644..4408
                     /locus_tag="KPE55_003251"
     CDS             3644..4408
                     /locus_tag="KPE55_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0494292.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4693..>6197)
                     /locus_tag="KPE55_003252"
     CDS             complement(4693..>6197)
                     /locus_tag="KPE55_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0494293.1"
                     /translation="NKALYINDVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPAN
                     LNANLRDYQINGLNWFKVLDYYKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPT
                     SLIYNWKNEFEKFAPDIKVLLIHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSE
                     IKFDYCILDEAQNIKNPVALVTESVKSINAENKFALTGTPMENNLLELWSIFDFIMPG
                     YLYSKAKFQELFINKEDNVKNLKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQ
                     KKIYSVYSKDIQDKMKDKNLKKDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETC
                     LEILRDSINENHKILLFSQFTSVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFN
                     NSMDKKVFLISLKAGGTGLNLTSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVI
                     KLIAKGTIEEKIIKLQESKKELINQFINGELSNEGVLKSLSDEEIINLFN"
BASE COUNT         2476 a          613 c          945 g         2163 t
ORIGIN      
        1 agtaatagag tttatactaa tgtaaataaa attgtattca aacaattgaa tataagtaat
       61 actttaaaag ctcctaaaaa tctaagagat atattaaaag taaatgtaaa tagtaaaata
      121 tttgatgtta aacctatgaa aaatcttata aacaaaggta gtaatttgaa attatgtaac
      181 ttgatagttt ttgggtgtat agaagttgaa atagactata gttgtaaaaa taggagtaat
      241 tttaaaacag taaataagtc taatgacatt aaaaaagata agtattataa aaatgatata
      301 caaaataaca atgaaaatat aaacgattgt aaaaagcaaa taagttttgg tgataataag
      361 gtaaaaaaaa taacaaaaac ttttggattt tcttgtttca tttgttctcc agttggaatt
      421 atttatgaag atatgaaaaa tataaatata aaaattgaac atacatcaat aaatgaatta
      481 aatccagaag aattatttgt aaatacatca cttttattgt attattaagt attttaaagc
      541 taaaacttaa acttattaaa atagcatttg gttaaaggaa aatagatatt gatatattta
      601 tcatatatat aaatgattaa ttaaacaatt ataaataata agtatagtat aatatttaaa
      661 aaatatagat ttatgataaa ataagacaaa tatgttaaaa aaatgataat cacggagaac
      721 ttttaccttc ttcaattaat atattattat tagtaataac tttaaaatta tattactatt
      781 atataaaaat tggcttaaaa gaaggtgaaa aaagtaaatg aaaatatgca taacagtagg
      841 tcatagtata ctaaaaggtg gtaaaagtac aggtgtaaat ggtatagttg atgaatacaa
      901 ttataataaa aaattagctc ctatgttagc tgagatgctt ataagtcaag gtaatacagt
      961 agatgtaata atttgtccag aaaggcactt tatgtcagaa agagaagaat ttttctatag
     1021 agttccaaaa gtaaacagtg gtaaatacga tttattagta gagctacatt taaataaagc
     1081 tgatggaaca caatgtggta cagaagtgct ttactatggc actgaaggat tagaatatgc
     1141 taaaagagta tctaatagac ttggagaatt atttgaaaat agaggagcta aaaaaagaga
     1201 aaatttatat atattgagaa atacaaatcc agttgcaata cagattgaaa gttttttctg
     1261 tgataatgtg aatgattgta acaaagcaaa tgaatcaggt tatgattata tagcacgatt
     1321 gattacagaa ggtatattga ataaagatat aaatatgtga gattatggtg atttaggtat
     1381 tgattaatac cttttaatat tagatataaa taaaaataca atgtattagt ataatttaaa
     1441 ctttcaaaag atagattagt ttgattattg acaagagata cctccttgaa aatattgatt
     1501 tatcagtata tttaaggagg tatttaaata taaaaattta attaatttgc tactaaacta
     1561 taggtttata gagtatatac ttttatcttc agtctaataa ataatataaa aatagtagaa
     1621 ccttgtatta taataatata atgtagaaaa atatatagat aagataaata taataaggtg
     1681 gtaatatatg aaatatacat ttgaaaaatt taagttaaat gaaaagatac ttaagtcatt
     1741 gaagagttta ggatataata taccttctag agtacaaaga gaagttattc caaaattatt
     1801 aaaaggtcaa aacttagtag ttaggtcaaa aacaggaagt ggaaagacag caagttttgc
     1861 aataccttta tgcgagaata taaatgtaga ttacaataat gttcaagctc ttatagtggt
     1921 tccaacaaga gaattagcct tgcaagtaaa agatgagata tcagatatag ggagactaaa
     1981 gaaggtgaga tgtagtgcta tatttggcaa acaatctata aaagaccaaa tagctgaatt
     2041 gaaacaaagg gttcatattg ttgtagctac tccaggcaga atacttgacc atataaatag
     2101 aggttctatt aagttggaaa atgtaaagta tttagtaata gatgaggcag ataaaatgtt
     2161 taataaaggt tttgttgaac aaatggaaaa aatattatta aatttaccta aggagaaaat
     2221 agtaagtctt ttttcagcta caatagatga agagataaaa tatatatgtg aaaaatatat
     2281 gctagattac agtgttataa atatagaaga aaatgagagc gatactaatc aaaaaacaag
     2341 acaaatagat gacaaaataa tcaaggcaaa tggaagagag aagtacatcc ttttaaaaga
     2401 attaatatac agcgaaaatc caaagagtgt gataatattt tgtaatacca aagagaaagt
     2461 atctaaatta tataataaga tgagtaaaga aggtttttta ataagagagt tacatgcaga
     2521 tttatcacaa gaaagaagaa tttttgtaat taaggatttt aaaaaccaaa aatttaatat
     2581 attagttagt agtgatgtag cgtccagagg aatacatatt gatgatattt ctttagtaat
     2641 aaactatgat gtgccacaag ataaagaaaa ctatatacat agaataggta gaactgggag
     2701 aaaaggaaat agtggaaagg caattactat agtaactgaa aaggatgaaa aatatataga
     2761 gaatatagaa acgtatatag gatataaaat aaatgaatta aaagatatag aacaaagtag
     2821 aattacacat ggcaaagttt tgtttgaaga atattcaaaa ggagtactaa aagaagtatc
     2881 aaagagaaaa aaagttgata aaaattctta caaaagtaaa gatataaaac taaatataga
     2941 aagtgaagtt gttaaattat atttaaatgc tggtaagaag aagaaaataa gagttttaga
     3001 tatagttggt gcatttagta atataaaagg tataactaat gatgatatag gtgtaattga
     3061 agttcaagat ttatgttcat atgtagatat attaaattat aagggagatt tgatattaaa
     3121 aaaatataag gagataccta taaagaaaaa aatggttaaa gtgaaaagag atatacgata
     3181 atttagtcaa tattgatgaa ttgctttgtt atcgaaatga agttgcaaaa aacacaatag
     3241 aagttgttaa ggatttgaaa gctgaagata tgaaacgaaa ggtctctcca attgacttag
     3301 aaaggatttt ggtgactgga ggagtaataa agcatgaaga ttctatttgg ttgttggact
     3361 tttgggggaa caaagatatt gctggaattt tgcttatgcc tcctactcgt catgttatac
     3421 ttcatttgaa tgattgttgt aaatggaagg aatattttcg tacaaaaaag aaattttaca
     3481 gaagttaaat tataataaat tagacaaaca atagataaat tagcaaggaa tgttgatttt
     3541 atctatattc taagttatac tatttttaag tggctaaggg aatagtcatt ttaaatataa
     3601 gtaataaata tatacctata gggatattaa aggggggaaa tatatggcat attttaacta
     3661 taacgacaaa cagtgctact ataatgaaat tggggatggt acaccattac tttttttaca
     3721 tggaaataca gcctcttcca agatgtttaa tgatataatc gatttttata aagatgaata
     3781 taaagtaata ttaattgatt ttgtaggtca tggaaaatct caaatggtcg ataaattttc
     3841 tgctgactta tggtttgatg aggctatgca agtgatatgt tttttagaag caatgaacta
     3901 taaaaaagta aacataattg gtagtagtgg aggcgctctt gttgcattaa atgttgcact
     3961 tgagagacct gatttagtga ataaggtaat tgcagatagt tttgaaggag aagttcctct
     4021 agagtcattt gtacaaaatg tgaaaataga gcgtgaagca tctaagcaag atgatggtgc
     4081 aaaagctttt tatatttata atcaaggaga aaactgggaa aaagttgtag ataatgatac
     4141 agaagctatt tttgaacatt ataaaactat aggaaagttt tttcataagc ctctagaaac
     4201 tatgcaacca gaggtgcttt taacaggaag tagagaagat gagtttgtat ctctaatatc
     4261 tagtgatttt tttgaaaata ctttttcatt acttcttgaa aagattaaaa atggtaaaat
     4321 gtatttgttt gataaaggtg gtcatcctgc aattttatcg aatggattag atttttcaaa
     4381 tgtagcaaag aaatttttag aggaataatt taaccaaaat ttacaataat gaaaactgta
     4441 aaatggattg caaaacttac aaaagtgagt gtacatatac ttcattatta tgatgaaata
     4501 gtattgttaa aacaaaatat tctaatagtc aagttattac atttgtatat gcaatatgta
     4561 tagaaagaga tatcattaga ttgtaaagtt gggattaaat aacaatatta aattttattt
     4621 attcagatat tttagcttaa ctatataaat gttagaacaa atttatatag ttaagctgta
     4681 ggattaaata atttagttaa ataagtttat tatctcctca tcagatagac tttttaatac
     4741 accttcatta gaaagttctc catttataaa ttgatttata agctcttttt tactttcttg
     4801 taattttatt attttttctt ctatagtccc tttagcaatc aattttataa cttgtactga
     4861 atttttttga ccaaatctat gagctctatc actagcttga ttttctactg atggattcca
     4921 ccatgggtca aagtgaatta ccatgtcagc agatgtaaga tttaaaccag ttccgcctgc
     4981 ttttaaagat attaaaaaaa cttttttatc catactatta ttgaactcat ctacaagttc
     5041 aagtctttct ttggcattag ttttaccatc tatataatgg tatttaattt tatacttatc
     5101 aagttcctta cttatgttct taagcacaga agtaaattgt gaaaacaata atattttgtg
     5161 gttttcgttt atactatccc taagtatttc taaacatgtt tcaatttttg aacttttttt
     5221 attgtaatct tttactacta tacttgggtc taaacaaagt tgtcttagtt tagttaaata
     5281 agaaaatata actattttat ctttctttaa atttttatcc ttcattttat cttgtatatc
     5341 ttttgaatat acactatata tctttttctg ctctttattc aattcaacaa aaaagttttt
     5401 ttcaatctta tctggaagtt ctttcataac ttgtttttta cttcttctaa gaataaaagg
     5461 ttttatcaat tttttcaggt ttttaacatt atcttcttta tttataaaca attcctgaaa
     5521 ctttgcttta ctatatagat agccaggcat tataaaatca aatatagacc ataattctag
     5581 tagattattt tccataggtg taccagtgag tgcaaattta ttttctgcat ttatactttt
     5641 tacagactca gttactaaag ctactggatt ttttatattt tgagcttcgt ctaatataca
     5701 ataatcaaac tttatttcac tatatttttc taaatcattt ctaagagtac catatgttgt
     5761 aagtattaca tcaaaatttt ctaattccat aaaacatttt tctctatctc ttttatttcc
     5821 atgtatcaat aaaactttta tatctggagc aaacttttca aattcatttt tccaattata
     5881 aatgagtgat gtcggtgtaa caataagact ttttttatta gataatgata ataaaaatgc
     5941 aatagtctgt attgttttac caagacccat ttcatcagct aatataccac caaatttata
     6001 ataatctaaa actttaaacc aattgaggcc atttatttga tagtcacgaa ggtttgcatt
     6061 taaatttgcg ggaatttcta aatttatgct atcaaaattt ctaaattttt cacatatact
     6121 gtctagttct tttttaccat ctataaactt taaattttta ctacgtatta catcatttat
     6181 atatagagct ttatttt
//
LOCUS       DAELNY010000202         6192 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.205, whole genome shotgun sequence.
ACCESSION   DAELNY010000202 DAELNY010000000
VERSION     DAELNY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.205"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1789)
                     /locus_tag="KPE55_003253"
     CDS             complement(<1..1789)
                     /locus_tag="KPE55_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494294.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDNKEKLEAVLEDSQIIIYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNY"
     gene            complement(1912..4455)
                     /locus_tag="KPE55_003254"
     CDS             complement(1912..4455)
                     /locus_tag="KPE55_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG0494295.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(4733..5067)
                     /gene="rnpB"
                     /locus_tag="KPE55_003255"
     ncRNA           complement(4733..5067)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPE55_003255"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5199..5876
                     /gene="srtB"
                     /locus_tag="KPE55_003256"
     CDS             5199..5876
                     /gene="srtB"
                     /locus_tag="KPE55_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG0494296.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSNINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(6036..6110)
                     /locus_tag="KPE55_003257"
     tRNA            complement(6036..6110)
                     /locus_tag="KPE55_003257"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6076..6078),aa:Thr,seq:tgt)
BASE COUNT         1918 a         1025 c          578 g         2671 t
ORIGIN      
        1 tatagttttt ccacaataac tcaaataact taaacatttc cctactagtt tcatctacat
       61 aataaggata catattttct actaaatggt ttaatcttga tatggcttgt tttaaaaata
      121 catcaagact tgcactttca ctttctttta acattctata attgtatttc ttcttatctt
      181 ctcctgaaat tacaaagatg atattgtcta aacttttttc aacttcattt aaataactgc
      241 ttatattata tgataaatca gacaatagat ttttaatctg cttaaacact tgtacatcaa
      301 gtttagtatt atacttttca aacaaatcta tttcttgatt ttctaatatt cttggcatat
      361 atgctgacaa taaatctaaa ttgtttcttt gttcttgtat ttgcttataa agtgaattta
      421 taacttctat tcttatttca tcatttaact ctttttctaa aatcttatca aattcagatt
      481 tcatatagta aatatttcca aagaaattaa atattatcat atcattcata gctttagacc
      541 aattcttata tatcacttca atagaatttt ctttaagtag aggttgcttt gcatatataa
      601 atacatcttg cataagtttt tgaaccatac ttttacttaa gtttaaaagt actttttgat
      661 tattaaattt tatttccgtt tctacaaaat tagaattttc gttttcaagt tctttattaa
      721 ttacaatgtc ttttatatca ctatttttta tagatatgat ttctttttga aaagacttta
      781 gaatagtcaa gctatcgaaa tcttcttgaa taaaaaatgg agcatgtttt aatgtatatc
      841 ccactatttc attttctata tcttttaggt catctatttg cataatttct ctatttttat
      901 catttatatg taggttaaat acatttttat tatcatataa tattatatct tctcctaaat
      961 ttaacttaga acaattatct ttcgctgact ttacaatagt cttcttagat ttcttattta
     1021 taaaaattaa ttggtcttga tattttacta aaagcatgtt tgtgttttta taatcaaatc
     1081 catctatatt tcctgatatc ttgcagaata ctatttcaca atctttagga agatttccta
     1141 ttttttggtt atccttaact ctctcattta aatcagatgg gactacacta tctatattgt
     1201 tattaaatat agatacagac cttactataa tcccctctct ttccatatga aagtatccct
     1261 tcaatgacac tcttttatct tctaaaatgt atctttctat atcatcaaaa tttatttcaa
     1321 atctattttt cccactgata aatataaact tctcattatc tttattaatt ccacccatat
     1381 aaaacttatt gtcatctaaa cttaatatag tcatattatc ctttagatag aatataaatt
     1441 ctctttgact tttgcagatt aaatcatatt catccaaagt tattttcata gtcaaatttg
     1501 ctgtaacatt tttacttgaa ttttcacttt ttatgtaaaa aggcaagtta tttccataga
     1561 taattatttg agaatcttct aaaactgcct ctaatttttc tttattgtct gaaaaactat
     1621 atatatattt tacacctgta tccttattaa cttcatctga tttgctgatt attatattcc
     1681 ctagatttgt tatatgaagc tttttatatt cttttgagta tctacttatg ccactaagct
     1741 caaacccatc tgaaagctca tatatgattt tttctcctaa tttcaacata ccttcaccac
     1801 ctaaacagaa ttataagtat tttataaagg tgaacaaaat gtctcacaat aaataatatt
     1861 ttttttaata ctttttattc tgagacatag tttctccttt tctataatgc tctaagcacc
     1921 ttcaactatt tcttcataaa gtttatctaa atgtttaaca tattcacgat atccagattt
     1981 gttctttaac tcacttctta aatcttcata tttttgttta agttcttcag cactcaaaga
     2041 aggagctgat tcttttaatt cttctaactt ataatcatag tacttatcat acatagctat
     2101 atatgtatca atagatttct tagctagttc attacatttt tcttgtgctt ctccatattg
     2161 agaataatca cctattttta ttttatagta tccattttct tgaattattg ttgaagaaat
     2221 acctttagcc tcatattctt ttgctgtttc ttttgctgtt ttttcaactt taagtgaacc
     2281 tgcataaaca gtgtaatata cacctttacc atctttatat gcttccattt cttttgcttt
     2341 cttttcagct tgtgcttttt cttgagcctc tttttctgct tttgcctttt cttctgcttc
     2401 ttttttctct ttttcagctt gtgctttttt ctcttcttca gttaatttat tgttagcttg
     2461 ttgttgagtt gatgactttg gaatattctc tttatcattt ttcttattgt tgcctactgt
     2521 tataaaatat cctacaccaa caataaccac tataactact gctataatag tttcttttaa
     2581 tctactcttc ttagaaacaa cctcttcatc atctaaagaa tcttctttat ctgatacata
     2641 atttttcatt tctttcttgt tttctctata tttgtcctta gaagaatttt tagatttttt
     2701 attactattt tctttctttt taataggtat tactactgat ttttcatttt ctaatttttc
     2761 ttcttttatt tcttttgctt ttttatcttc taagttttta ctttcttttt caagcttcga
     2821 ttcattattt gaagatactt tttcatctaa gttattattt attggttttt ctatttttac
     2881 attacgttct tcttctatat gttgacttgg ttcttcttgt tttatttctt ttaattcatt
     2941 ttgctctatt tcttctgatt ttacttgatg attttcaact ggtttatcta aaattatatc
     3001 aggagaaata tcatttaaat tctcttcttc ttgttcaact tcttcattgt cttttttaat
     3061 aaatccaaat aatttagcct tctttttact ctttttattt tctggttcaa ttatatcttc
     3121 tttattgctg tcgtactctt tatctgtagt atctttatct tctacaacgt cacctatatc
     3181 ttgattatta ctatctattt cttctttaac cccttcttca gcatcatctt ctattgaaat
     3241 actacttcta aagcaaatat ctttatctgc attttttatc atgtaaatgt attcttcaat
     3301 atcgatatct ttatttcttc tagttctttt tgatgcaatt tcatgatttt ctaattcact
     3361 atagttatct tttattagat tctgtgcttt attccatgta tcttcactaa tttctgtttt
     3421 atttttaatt acatagtcca caaattcctt aaaattttca ggaccaaatt ctccaattat
     3481 agtcagtctc attgcacctt ctataaggtc tctatttctt ctctttaaaa attctaatac
     3541 aaccgttgca caagcaaaat ctttaataat ttctttgcca aaaccatatc cttcaacaga
     3601 atgaagttct tctaatccac ataacttctt atattcattg tgtattgttt gagatacact
     3661 ttcattattt aagctattta acttatctat aaacttcgat tctatatcat ttgattttga
     3721 ttcatgtttt acttttgaat ttttaatttc atttaatagc atgtcatttt ctgaattatc
     3781 tttacctaat tgcaaatcct caatttcagt tgcttttatt tctactttgt cttgttcgtt
     3841 attctcttcc acattattag gtaccacatc tttattgcta gctaatattt tgtttacaca
     3901 gaatataatt tctcttttta attctctttt cgcatctgct tgatttttaa gaaataaatc
     3961 tccacattct ttcttttcta aacttatttt tctaagagat atattaagaa gatctttata
     4021 taggtttaaa accttataat ccttttctaa acgtcttctt ctctctgata gttccatttt
     4081 atcataagct aaaaaactat ttaaatcatt ggcttttaga gagtctaatc cctctttcat
     4141 agccttattt acatactttc tacattttgc tgtagttaat tctaaatctt tttctaattc
     4201 atctatttca ggtatgtact tatctactat ccatttgcca ataagtggtt ctgtatttct
     4261 tgctaatgtc ttataaaaca atatattaaa ttttaaaagc tctgcatatt ttgcttcatt
     4321 aaattctact ttttcaaatt tcttttccaa aacaccatct agcttttcat ttattttaga
     4381 ctcttgtttt aaaaaacttg ctgtgacatt taaaacagtg tttcttatta tattattttc
     4441 actcaaaacc ttcactcctt aaatcaatta ttttattgta atatttacat tgtgcaaagt
     4501 tgctaatttg attatgatat ctaaataatt atttactaaa tttgctacat cttatatcat
     4561 atcatattat ttacatcatt ttactttttc tttttatatc tatcttatat tataccattt
     4621 ttaatatctg tactaactta tattacaata ttcctcaaaa ttttaaaagt agattagaca
     4681 aatttacaaa taaaaaaaca tcagtttaaa ctgatgtttt tgactatcga aataattaag
     4741 caagtctata agcggagttc tgtattagat aatcatcttt ctaggcctat tgtcaccaat
     4801 aggctcaagc gacctaccta ccggagagta cgagcagcac tctttattcc tatcttggtc
     4861 ttgctccagg tggggtttac atagccaact agtcgcctag ctgctggtga gctcttacct
     4921 cgcctttcca tccttaccac aatagtggcg gtttatttct gttgcactat ccttaggatc
     4981 gctcccacta ggcgttacct agcaccctgc tctatggagc tccgactttc ctcatgtaag
     5041 attacatgcg attatctggc ttacttaata tttattttac ttagttatgt taacacaaat
     5101 tcttttacat gtcaaagtaa tattatacta aaaatttaaa tttattcaaa aatagtgtaa
     5161 tatagatata taaactacat ttaactcgag gtgatatatt gaaaaaatta tatagaatag
     5221 taattaacat tatactagta ttagtaatac tttacagtgg atttaatata tactcaaaac
     5281 ttaccaaata caatcatgat actaaaatat cctcagaact tcaaaaaaaa gaatataaaa
     5341 aagaggattt atcaaatatt aacagtgatt ttaaattttg gctttctgtt gagaatacaa
     5401 atataaatta tccagttgta caatctaaag ataattctta ctatcttgac aaagattttt
     5461 ataaaaaaga ttctatttct ggtacactgt ttatggatta tagaaataaa tctattgatg
     5521 acaaaaatat cataatatat ggacataata tgaaaaataa aactatgttc aacaacctaa
     5581 ataagtttaa agatgctgac ttctttaaaa aaaataataa aataaaaata accttgaatg
     5641 gaaaagaatt tttatatgat gttttttctg cttatattgt agaatctgat tatgattatc
     5701 taaaaacaaa ttttaacaat gaatctgatt atcaaaatta cataaatgat attacttcta
     5761 aatcattgta taaatctcct attaaggtaa atagcaatga taaaatagtt acactttcta
     5821 catgtaccta tgaatttgat gatgccagaa tggtgattca tggtagattg atttaaaact
     5881 cacagacaca gtttaaaaca ataattttaa actgtgtcat cttttatacc tataatttgt
     5941 aaattagacc actaaaagta tttaatacaa aataacaaat acatcaaaca ataaaaaaca
     6001 ataaaaaaag actatgctaa aaacatagtc tttactggag ctggtgatag gaatcgaacc
     6061 tacaacctgc tgattacaag tcagctgctc taccgttgag ccacaccagc acacctattt
     6121 tattgtaaat tagcaaaact aatttttaaa agttaacact ctaaaaacta cacatatatc
     6181 ttaatgaatg aa
//
LOCUS       DAELNY010000203         6135 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.206, whole genome shotgun sequence.
ACCESSION   DAELNY010000203 DAELNY010000000
VERSION     DAELNY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.206"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            72..515
                     /locus_tag="KPE55_003258"
     CDS             72..515
                     /locus_tag="KPE55_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0494297.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            546..2363
                     /gene="ftsH"
                     /locus_tag="KPE55_003259"
     CDS             546..2363
                     /gene="ftsH"
                     /locus_tag="KPE55_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0494298.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFEESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2541..5243
                     /locus_tag="KPE55_003260"
     CDS             2541..5243
                     /locus_tag="KPE55_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG0494299.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKDIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5277..5975
                     /locus_tag="KPE55_003261"
     CDS             5277..5975
                     /locus_tag="KPE55_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494300.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         2405 a          694 c         1141 g         1895 t
ORIGIN      
        1 gttaagtatt tatttacagt atgcacttca gcctaaattt gaaataaagt attttcgtag
       61 ggagcgtttg tatggatgag atgagtttta agaaagagct actagagttg actagagata
      121 taaatatgaa atttacaact ttattaagtg atttttatca gccacttggt attacagcag
      181 tgcaagcttt gattttatct gagttatgtg aacatggaga gaaaaaaata agcgatttag
      241 ggaagaactt aaacatgaca aatagtaatg tatcagtaat ctgtcaaagg cttgaaaaaa
      301 atggcttttt aaatcgcata agagatatag aagaccaacg aattgtaaaa gtaaaagtta
      361 caaataaatc attggatata caagaacata tttcaagtag tatttttgat agttattttg
      421 aaaatatgac cagtgaaaaa ttgcaagata tggaagatat aatagaaggt ttagagaaat
      481 taaataaact tttaacttac attgattgca aataacataa aaataattta aaggagaata
      541 aaattatgga tgaacataaa ccccaaaaaa ggtcagcact ctattattat acagttataa
      601 taatagtagt aatgttttta aatgctttaa tatttccaag tttaatggat aaaaaaatgg
      661 ttgagattga ctatggaaag tttctcacaa tgttggatac aggaaaggtt aaatctgttg
      721 agatagaaaa taatagaata gcaataaatc catctagtag caaggataag aatatataca
      781 taactggtag aatggatgac ccagaattgg tagatagact aaaaaatgca aaagtagaat
      841 ttactaaggt tattcctaag gaaaattcac ctcttttaag tattttactt acatggataa
      901 tacctatagg tattcttatg ctatttggta acttaatgat gaagtctatg caaaagcgaa
      961 tgggtggtaa tgctatgcaa tttggaaaaa gcaacgccaa aatgtatgta tctgctcaaa
     1021 gtggaaaaag atttaatgat gtagctggtc aagatgaggc aaaagaagct ttaactgaaa
     1081 tagtagattt cttgcacaat ccagaaaagt ataaaaagat aggagcacaa atgcctaaag
     1141 gagcactttt agtaggacct ccaggtacag gaaaaacact ccttgcaaaa gctgtagcag
     1201 gtgaagcaaa tgtaccattt ttctcaatat ctggttctga gtttgttgag atgtttgtag
     1261 gaatgggagc ttctaggatt cgtgatttat ttaaacaagc aaaagaaaaa gctccatgta
     1321 tagtgtttat agatgagatt gatactattg gtaaaaagcg tgataatgga agtggattag
     1381 gtggaaatga tgaacgtgaa caaactttaa atcagttgtt aagtgagatg gatggttttg
     1441 atggtggaat tggagtagta atacttgctg ctacaaatag acctgacagt cttgacaaag
     1501 cattacttcg tccaggaaga tttgatagac gtataccagt tgaacttcca gaccttggag
     1561 gacgtgaagc tatattaaag gtacatgctc aaaatgtaaa tgtaagctca gatatagatt
     1621 ataatgcaat tgcaaggtct acatcaggag catcaggtgc agaacttgca aatattgtaa
     1681 acgaagcagc acttttagca gttaagtctg gcagagatat agtagaacaa caggattttg
     1741 aagaatctgt tgaaactgta attgctgggt atcaacgtaa gggagctgta atttcagata
     1801 aggaaaagaa aattatagca tatcatgaaa taggtcatgc acttgttgct gctatgcaaa
     1861 aacattctgc acctgtccat aagattacta taatacctag aacttcagga gcattgggat
     1921 atactatgca agttgctgaa gatgagagtg tacttatgtc taaagaagaa gcaattgata
     1981 aaataactac ttttacaggt ggtcgtgctg ctgaagaggt tatctttaac acttgtacat
     2041 caggagcatc aaatgatata gaacaagcta ctaaaattgc aagagctatg gttactcgcc
     2101 ttggtatgag tgaagagttt gatatggtcg ctcttgaaac tgtaagcaat caatatttag
     2161 gaggagatac ttctcttgct tgttcttccg acactgctgc taaaatagat gaagaggtat
     2221 ttaaaatagt aaaggcttgt cacaaaaatg caattgaaat cttagaaaat aatattgata
     2281 aattgcatga acttgctaat tatttattag aaaaagaaac tattacaggt gatgagttta
     2341 tggaaatact taacttaaac taaaatgatt atacacaaga taaaaatgcc tattgttagg
     2401 catttttatt ttacaactaa attttttaaa ttcatattta aatctttaca taatcttaat
     2461 cattaaaaat acatcttaac tacaatttta tatcggaatt gatattataa tagtaatgaa
     2521 tggtgtaagg aggtttttta atggatagac ctaatcctga tattctatta gaaaaaatta
     2581 aaaatgaaga agaaaaacta agtagaggtc aattaaaaat attctttggt tatgcagctg
     2641 gagtaggaaa aacctactct atgttggaat ctgcacaaaa tttaaaaaaa gtgggtgtgg
     2701 atgtagtagt tggatatatt gaaccacaca ctagaccaga aacattggct ttacttgatg
     2761 gcttggaaat tcttcctgta aaggatatag aatataaatc tataaaacta aaagaatttg
     2821 atttagatgc tgctttagca agaaaaccag aagttatatt agtagatgaa tttgcccatt
     2881 ctaatgttgc tgggttaagg catacaaaaa gatggcaaga tatagaagaa ttgctgttag
     2941 caggcataaa tgtctacact actgtaaatg tacaacattt agaaagtcta aatgacattg
     3001 ttgagataat tacaaacgta tcagttaaag agactatacc agataagttt ttggatacaa
     3061 atacacagtt agaacttata gatgttgaac ctgatgtact tcttgaaagg ttcaatgaag
     3121 gtaagatata taagaaagaa caagctacta gagcaaaaaa taattttttt ataaaagata
     3181 acttggttgc acttagagaa atcgcactta gaaaaacagc agagagagtt aataaagaag
     3241 ttcaaatgac cagactatca aagggtgatg taactgtaat acctacatca gatacacttc
     3301 ttgcatgtat aagtccatct ccatcttcag caaaagttat tagaactgca tccagaatat
     3361 cagacagtac atttgcaaaa tggatagctt tatatgttga aactccaaac acagcaagac
     3421 ttccatttga tgaacaaaaa caactacaaa ataacctaaa attagctaag aaattaggtg
     3481 gagaaataat tgttttacat ggtgaaaata taatagagca aatacttaga attgcaaaac
     3541 tgagaaatgt aacaaaaatt gtaataggta gaaatcattc aagtaataaa agatttagta
     3601 aaagatttaa aaaagatata gttgacaaat taattgatga agttgattat atagatatac
     3661 atataattcc atataaattt gcctcagatg ttaaatacaa acctaaaaag gataaatcat
     3721 caataaaatc taaatttaaa attagcaaag ttgattttat aaaacttata tttattactt
     3781 tgatgataag tattttagca tatgcagtac aaagcatggg atttataaga gaaaatatat
     3841 tgttaatata tatgttgggt gtagttttag tttctatgtg gactaaaggg tatagtacag
     3901 gtataataag ttctgtattt aatatagttc ttttaaatta tttttttaca gcaccattat
     3961 atacattatc tatagcagat tcaaactata tagtaacact ggttgtattc tcaatagtag
     4021 gtataataac tagcactctt acgtctaaga tacagcatga agcagaaact gctgctaaaa
     4081 gagaagaaaa tacaaaaatg atatatcaaa taattaaggg atttttaaga ttatcaaata
     4141 aagatgatat agtaaacaaa ggtatagaac ttttgtctct tagcttaagt agagatgtaa
     4201 tttgttattt aaatgtagat aagcataata aatcaaagct ttacaaaaaa aatactaata
     4261 ataaggataa aaatgatttg gatagtgaag atgaaaaagc tgttgcaagt tgggttttat
     4321 ccaattcaac agtggcagga aatgacacgg atacacttcc tggttcaaag ggatactata
     4381 taccaattgt aggaatgaat ttaactttag gagtagtggg aatatcatgt attgattcaa
     4441 aattggatac agaagatata agcttaattg aaacaataat tgctcaaatg gcaatagcgc
     4501 ttgatagaga aatcttgtca gaagctaaag aaaataccaa tcttgagatt gaaagggaaa
     4561 gattaagaag taatctttta agagcagttt cgcatgacct tagaactcca ctagcaggaa
     4621 tatctggtgc agtaagtaca attataaaaa acaagggtac tataggtcaa gatataatag
     4681 atgaattact tagtggtgta tatgaagata ctcagtggtt aataagactt gttgaaaatt
     4741 tacttagtat gaccaagata gatgaaggga aattggaagt taaaaaacat tctgaactgg
     4801 ttgaggaaat tatatcagaa gcgttacaaa aaataaaaaa aagaattgaa aatgcactta
     4861 tagatattaa tataccagaa cagatattat ttgttcctat ggatgcaaaa ttaatagaac
     4921 aagttttaat taatttaata gataattctt tgaaatattc aaaggaagat tgtaagatag
     4981 agataaatgt atatgagaaa gatgattatg tttggtttga agtatctgat aatggtcctg
     5041 gtatatcaaa ggaacttaaa aagcatatct ttgacagatt ttttactgga gaagaaggag
     5101 ctaaagattc cagaaagggt gttggacttg gattatcaat ttgtaaatct ataattcaag
     5161 ctcataaagg tgaaattatg gtagaaaaca ataaagataa gggttcaact tttaagttta
     5221 gtttgcctaa ggaaaatgaa tagttttaaa ttttaaaatt agttttggag gaagaaatga
     5281 gtaaaaaact tatattgtta gtagaggatg ataagacaat aagaaaattt atatctactg
     5341 cacttttaac acaggattat gatgtcaagg aggctataac tggaaaagaa ggtatatcta
     5401 tagctgtctc atattctcca gatgttgttc ttttagattt aggcttagag gatatggatg
     5461 gaatagaggt aataaaagct atcagacaat tttcaaatat accaataatt gtagtttcag
     5521 ctagagagca agatagagac aaggtagaag tatttgatgc aggggcagat gattacttaa
     5581 caaaaccatt ctccatagta gagttattag caagagtaag agtagcattt agacattcac
     5641 aagtagaagc tcaacaaaaa gatgatgtta aatctacgtt tgaagtagat aaattattga
     5701 ttgattttga taaaagaaaa gttattgttg atgatgttga agtccatttg acacctattg
     5761 aatataatat tctttcatta cttgcaaagc atcatggaaa agtgcttact cataatttta
     5821 taataaaaga aatatggggt agtgttatag ggaatgagac taagtcttta agggtcttta
     5881 tggcaacgtt aagacgaaaa atagaaaaac aacctgcaaa cccaagatat ataattacag
     5941 aagttggtgt aggatacaga ttaaatgatg aatagttgta atatattaat tattagatta
     6001 tgtgcttatt aatatatatt aaaattatat agtttagtta aaataagaat gaagtgtatg
     6061 tgcttgttgt cagtttagca catgcatttt attttttagc ttttttagta tcaataattt
     6121 tttagtatca ataat
//
LOCUS       DAELNY010000204         6116 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.207, whole genome shotgun sequence.
ACCESSION   DAELNY010000204 DAELNY010000000
VERSION     DAELNY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.207"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            133..699
                     /locus_tag="KPE55_003262"
     CDS             133..699
                     /locus_tag="KPE55_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0494301.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            739..1320
                     /locus_tag="KPE55_003263"
     CDS             739..1320
                     /locus_tag="KPE55_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0494302.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1377..2294)
                     /locus_tag="KPE55_003264"
     CDS             complement(1377..2294)
                     /locus_tag="KPE55_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0494303.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2424..4055
                     /locus_tag="KPE55_003265"
     CDS             2424..4055
                     /locus_tag="KPE55_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0494304.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4058..5005
                     /locus_tag="KPE55_003266"
     CDS             4058..5005
                     /locus_tag="KPE55_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG0494305.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5290..5553)
                     /locus_tag="KPE55_003267"
     CDS             complement(5290..5553)
                     /locus_tag="KPE55_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG0494306.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5652..5897)
                     /locus_tag="KPE55_003268"
     CDS             complement(5652..5897)
                     /locus_tag="KPE55_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494307.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         2200 a          830 c          986 g         2100 t
ORIGIN      
        1 attcatgtag ttatataata aataatttat aaatatagcc aacttagcct aaatacgtat
       61 ataatatagc taatatggtt cttatacttg ataccatcaa taaaactaaa aatataaaaa
      121 ggagaactta aaatgagtgt tagtttacaa aaaggacaaa gagttagctt agtaaaagat
      181 aataatcctg taaaaaatct agtagtaggc ttaggatggg atatgaataa attgggtaaa
      241 aaaaattatg acctagatgc atttgcaata gcattgacta accaagataa aatgaatgtt
      301 agaggtgact tggtttattt tggaaatctt aaacaccctt ctaaagctat tatacacact
      361 ggagataact taactggaaa aggtgaaggt gatgatgaac aaataattgt taatttagaa
      421 gacatacctg aatatgtaca taaaatagtt tttggagttt ctatatacaa agctaaaaaa
      481 agagaccagg attttggtca aatgaacaac tcttttataa gattaattga ttcaaattcc
      541 aagcaagaat tatttaaata taatcttcaa gaagactttt ctggtaaagt aacagttttg
      601 gcaggagaaa tttatcgtag aaatgaagaa tggaaattta atgctttagg aattggtcaa
      661 aatgaagagc ttcgtgaatt aattaataca tataagtaat aattacaata aaaaatttat
      721 aatgaggagt tggtttaaat gggtataact ttagcaaaag gacaaaaagt aagtttaaca
      781 aaaagtaacc ctggtctaaa aaaagtaata gtaggactag gatgggatat taataaatat
      841 gatggtggct ttgatttcga tttagatgcc tctgcatttt taactggaac tgatggaaaa
      901 gtaacgaatg atggagactt tatattctat aataatttaa aacacgcttc tggagctgtt
      961 gaatatatgg gtgataatag aacaggtgtt ggtgatggtg atgatgaaca aataaatgta
     1021 gacttatcta aaatacctca aaatattgct aaaatatcat ttagtgttac tataaacgaa
     1081 gcaattacaa gaagacaaaa tttcggacaa gttgaaaatt catatataag aatatataat
     1141 gaagaaacta atgaggaact tattaaatat gaattgggtg aagattttag catagaaaca
     1201 gcaatagttg tggcagaact atatagacat aatggagaat ggaaatttaa tgctttaggt
     1261 tctggatttg aggatggact tgcaggattg tgtaaaaatt ttggcgttaa tataggctaa
     1321 tatatctaaa taaaaataag agtatattga tatactctta tttttattta gatatattat
     1381 aaactgttta cacttctact cttctttctc gcttgttttt tcccatattc tactgaaata
     1441 tcaaaaaatg ctgaaatatt ttctgtaaaa aacttgcttc tatgatatgc aatatcaaat
     1501 gtcctatgta aattaatacc ttcaatatca caagcccaca aaagatgctt ttttaaataa
     1561 tcctcaataa gtaattcgga tataactgca atgccaaaat tatctacaac tgctcttaat
     1621 atagcttcag agttataaca tgaccatcta atattcattg ggatttttaa ttcttttaat
     1681 tgcttttcta attgtgctct tgtaccactt cctaattcac gcataattaa tggttgattt
     1741 gaaagttctg aaacttttat agagtctctt ttataaaact catgcttgtg tgaacatata
     1801 agtacaagat tatcattaat aatgcttttt gtaacaatat ctgggtgctt aatttttcct
     1861 tctacaatcg caatatctaa ttcactatta agtaattttt ttgcaatcag tcttgtatct
     1921 tctacaaata cttcaggttc tataagagga tttaccttgt ataattctaa cattattgga
     1981 cttattacac atgtaccaac agttactgtt gccccaactc ttatacggat attttttgca
     2041 caatgcttca tacaatcatt catttcttca tttacagcta aaagatgcct cccatatcca
     2101 agaagttttt tccctgcctc agttaaatat aatttttttg aaattctatc aaatagctta
     2161 acaccatact ctttttctat ttctgaaata gcttggctaa ctgatgactg ggtaatatac
     2221 atatttcttg ctacttcact cattttacca cattcagcaa ccttaacaaa gatttcaaga
     2281 ctacgaattg tcatattatc acaaccttag caaacattag taaatgctta tgattttatt
     2341 aatattttag tattttacaa atatattgtc aagaagtata attttattat agagttataa
     2401 agtagattgg agtgtgatat attgtgaaag tattaattat aggtggagtt gctgctggaa
     2461 caaaaactgc tgcaaaaata aaacgtgaaa tgggtgataa ttgctctgtt acaatactaa
     2521 ataaaggtga tgatatttct tatgcaggtt gtggtcttcc atattacgtt ggtaagataa
     2581 ttgaagataa atcatcttta atcgtaaata cacctgaaag tttttctaaa ctaacaggag
     2641 cagaagttcg ttgtggagtt gaagttacat ctattgatag aaatgcaaaa caagtaactt
     2701 tgaaaacgct ttcaacagga gaaaaagaaa atctaagcta tgacaaactt gtaattgcta
     2761 caggggcaga cccaataaaa ccaccaatac ctggaataga tttacctgga gtattcttta
     2821 tgcgtactcc aaatgatgct ataaaactaa gagattctat tgaaaacggt attaagagag
     2881 ctgttgttat tggtggaggt ttcattggtc ttgaaattgc tgaaaatctt tctgcaatgg
     2941 gtatacgtgt aagtgttgtt gatatggctg aacatgttat gcctggattt gatactgatt
     3001 ttgctgaata cgttgaaaat catatggctg ataaaggcat aatgattttt acaggtgacc
     3061 aggttgtagg tatcgaaggt gaaaataaag ttgaaaaact tcgtactaaa aatagagcta
     3121 ttaaaacaga tttagtggtt atgtcagttg gaatccgtcc taacacaggt tttctaaaag
     3181 acacaggttt agaatttgcc cctaataata caattattgc agatgaatat atgcttacaa
     3241 atgacaaaga tatatacgta gttggagact gtgcttttgt aaaaaattct ttaacaaata
     3301 aacctacatg gtcacctatg gggtcatctg caaatcatga gggtagaatt tgcgcacaaa
     3361 atatatctgg aaaatctaaa acttacaatg gtgttcttgg aacaacagta gtaaaattac
     3421 ctgagctcaa tgcaggaaaa acaggtctaa gtaaagaaat agctgaaaaa gaaggttata
     3481 atgtttgctc tgtaaccata gctactgatg ataaagctca ttattatcca ggtgcatcta
     3541 attttattat aagaatggtt gctgaaaaaa atactagaaa attattaggt gttcaagtta
     3601 tgggacctgg agcggttgat aaaatagtag atatcgctgc tactgcaata actttaggcg
     3661 ctgaccttta ttcattagaa agtatggacc ttgcatatgc accacctttt tcaacagcta
     3721 ttcacccttt tgtagtggca gtaaatgttt tacaaaataa acttaatgga gaacttgata
     3781 gtgttttact tgacgaaatt gaggattttg acagttggac taaacttgat gtttcaaaag
     3841 ctccttctat tcctagttta cgttatattc ctgttggtga aattaatgga gaaattgaag
     3901 gccttgctaa ggatgaaaaa attcttctta tatgtgctaa aggtaaaaat agctatatgg
     3961 cgcaaaatcg cttaagacgc tttggctata caaatacgaa agttcttgaa ggtggaatac
     4021 tattttatcc aaatcttaag gtaatggagg agtaaatatg agtaatgtta cagcagaaca
     4081 aaaaaaggaa gtaaaaggta gaggttttct accaagcagg gatggagaac attttgcagc
     4141 acgtataata actgtaaatg gtgtaataaa tgcatcacaa gcaaaaaaaa ttgcagaagc
     4201 tgctgaaaaa ttcggaaacg gtcagttagc atttactact agactaacag tagaattacc
     4261 tggtgtaaaa tttgaagata ttgaagctct aagtgaattt attgctagcg aaaatctaat
     4321 aacaggtgga actggatcaa gggttcgtcc tgttgtggct tgtaaaggta ctgtttgtgt
     4381 tcatggtcta gctgatacac aggcacttgc tgctgaaata cacgaagaat tttacaaaaa
     4441 ttggtatgat gtaaaactac ctcataagtt caaaattggt gttggtggat gccctaataa
     4501 ctgtattaaa cctgacctaa acgatcttgg aattgttggt caacgtgtac ctgactatga
     4561 ttcagagctt tgtgttggtt gtaaaaaatg tgctgttgta gaggtctgtc ctgttaaagc
     4621 agctaaatta acagataaag gtaaacttga aatagatagt aatctatgta ataactgtgg
     4681 taagtgtata gaaagttgta attttgatag tattgaagaa aaagaatctg gttataaagt
     4741 ctatatagga gggaaatggg gaaaatcagt tcgtcctgga acacagattg atagattatt
     4801 ttcaaaagag gaacttatga ctttaattga aaaagcaatt cttttattta gagaacaggg
     4861 taacacaggt gaacgttttg gaataactat cgatagaatc ggtgctgata aatttataga
     4921 aatgctttta tcagatgaag ttttggaaag aaaagaagag atactagagg ctcttttaca
     4981 ccttacaggt ggagctgtat gctaacaata aatattttaa ttgtacagat aacctatgga
     5041 aaaaatttta gttaagaata attaaaattc tatatatgat atatgataaa tgaggctatc
     5101 tcaaaacaca gatacgcctc tttttcattg ttgaaatgat aaagtatatt tgagttcata
     5161 taaaaaagag tgtctcgtga attataaaat tcattttgag gcacccacat tattttataa
     5221 taattgctta ttaattttat tttaactata taaaaagtac aaaaagtaag aatatccaat
     5281 attctaaaat taattttgaa ttgtattgat tgaaaagttt actattccac tatttccatc
     5341 ttgatttact actagatacc atgttccagt attaggaact ctcattctac atggattttt
     5401 ctttgcaaat ccaccatagt aatcataatt tctttgattt ttaaatttca cataatttat
     5461 atggtctaac aatattacat tggcttgttt ctctaaattt acctctacaa tatccccttt
     5521 ttttaagttc atcttactat aactatattc cattgaatta ctcctccagt atcgaataat
     5581 ctttgttatt tactcatctc aaatatgtaa aaattcattt aaaatattac aaatactata
     5641 taattagact attatccagt tttaacctta tttattacaa tccaaaggtt attaaaaata
     5701 ttattggcaa caacttctcc atcccataca actctttctt ctttattttc taataaagca
     5761 tagtacccag agtcagaact ttcatcttta tagactgtaa cataaaactc ttcatcacta
     5821 tattcaggta tatttttaaa gtcttcagat attttacatg ttaatatata accttctaat
     5881 tcttcatcta taaacaaagc aattcctcca atctctatat ttttactaat tataccatat
     5941 tatgtcataa actttttatt ttattacaaa tttctatttt ttctttactt tataaataaa
     6001 ttatataaat tttcactttt taactttata ttatactaat taattctttt attttcctat
     6061 atatttcttt ataatttata aatttagcca ttaatttata cgattttata taggta
//
LOCUS       DAELNY010000205         6097 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.208, whole genome shotgun sequence.
ACCESSION   DAELNY010000205 DAELNY010000000
VERSION     DAELNY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.208"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            174..1565
                     /locus_tag="KPE55_003269"
     CDS             174..1565
                     /locus_tag="KPE55_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0494308.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            1672..2712
                     /locus_tag="KPE55_003270"
     CDS             1672..2712
                     /locus_tag="KPE55_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0494309.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            3201..3854
                     /locus_tag="KPE55_003271"
     CDS             3201..3854
                     /locus_tag="KPE55_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0494310.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            3930..4139
                     /locus_tag="KPE55_003272"
     CDS             3930..4139
                     /locus_tag="KPE55_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494311.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(4279..4845)
                     /locus_tag="KPE55_003273"
     CDS             complement(4279..4845)
                     /locus_tag="KPE55_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG0494312.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            5006..5659
                     /locus_tag="KPE55_003274"
     CDS             5006..5659
                     /locus_tag="KPE55_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG0494313.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(5817..5993)
                     /locus_tag="KPE55_003275"
     CDS             complement(5817..5993)
                     /locus_tag="KPE55_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494314.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         2357 a          640 c          962 g         2138 t
ORIGIN      
        1 ttatttttac ttattaaaaa aaaattatac tagaaaagtt tggttttttg attaaagtgg
       61 tatataatag atatataaat taatatttaa aaagtagtta atattagggg agggcatttg
      121 cacctccctt gtaatatgtg gaaaattgaa tattagaaga aggaggagac ttgatggaaa
      181 atcaacaatt attaggaaca gaacgaatta gtaaattact actaaaatat tctataccag
      241 ctataatagg tatgttggta aatagtttat ataatgtagt agatagaatt tttattggaa
      301 atataccagg ggttggacca cttgcaataa caggtcttgg tgtaacaatg ccaataatga
      361 caataatact tgcatttggg atgttgatag gtataggaac tactactacc atatcaataa
      421 aacttgggca aggtaaagta gaagaggcaa gaaaacttat aggaaatgca atgacgttat
      481 ctgtaataac aggaattatc ataatgatac ttggaatatt atttgcaaat aaaatactta
      541 cattgtttgg agcaagtgaa aatactttaa tatatgcaaa atcatatatc aatattatat
      601 tattaggtac agtagtaaat cttctttcct tttctttaaa ccactcaata agagctgatg
      661 gtagtcctaa aatttcagca ggaataatga tagtaggatg tttaactaat atagttttag
      721 actggatatt aatatttgga ttcaatttgg gaatacaagg agctgcaatt gctacagtaa
      781 cttctcaggc tcttacagct attttaacta tagggtatta tataagtgga aaatcaaact
      841 tacgattcag taaatctaat ctaaaattag ataaaaagct aataaaagca gtgtttgcaa
      901 taggtatgtc gccatttgct atgcaacttg cagcaagttt agttcaagta atatcaaata
      961 ttgcacttaa aactcatggt ggagatttag ctataggggc tatggctact atttcttcta
     1021 ttgcaatggt atttttgatg cctatctttg gaataaatca aggagcgcaa cctattatag
     1081 gtttcaatta tggagctgaa aaatatgata gggttaaaaa agcatattta ggttcattag
     1141 tagtagctac tataatttta tgtatgggta tggtagtagt tatgcttttc ccagaagcta
     1201 ttataggtat atttaataaa gaccctgaac ttatgaatat atctgttaat ggattaagaa
     1261 tatacttact tatgttgcca attgtaggct tatcagttac aggaacaaac tttatacaat
     1321 ctataggaaa agcaaaaatg gctatgttat tgagcctttt aagacaagta attttattga
     1381 taccagcagt attaattctt cctacattct taggtttaca gggtgtatgg acagctcaac
     1441 cagtgtctga ttttatagct acagtaatta caggtatagt tgtatttaga gaattaaaaa
     1501 gatatactcc taaaactgaa aaattaaatg aaaatgaaag actaaacgag ataactactg
     1561 agtaaaaata taaattcaga taattgagca agaaattgca aaaaatacga ttatatgata
     1621 aaataaaacc gctgtcaagg ataagagaaa ttctaattat ggagtgattt tatggaattt
     1681 acaaagaaaa gcaaattaaa aaatatgtat gtaaacatga atgtagctag aatgttgtct
     1741 aagataaatg agtataaggg tagacaactg ctttacaaaa aacaaccaaa agaaatttta
     1801 gaaaacttag aaaaaaaatc tttagtagat tgttctgaat ctacatacat tggaaatcaa
     1861 agagatagta gtaattttaa tctagaaaag ttgataagta atgaagttac acctagaagt
     1921 agagaggaat tgagtattgt tgaatacaga gatgtggtaa aaactattaa cagtgcttat
     1981 gagagtattc ccatcagttc acaaacaatt cttgagcttc atggatactt atataaattt
     2041 tcatctacta gaggaggaag ttataagtca gataatgatt ttattgaaca caatttaaaa
     2101 cctagcgaat ttataagtac tgattcaagt gtcaataaag ttgaaaaagc tgtagaagaa
     2161 atatgtgaag cttataatac attgatagag gaagatgaaa ttgatatatt aatattaatt
     2221 tctgcatttg tgttagattt catattgata catccattca aagaaggtaa tataaaaatg
     2281 gcgagagtac ttatcctatt gttactaaat aaaaatgggt atgaagttgg aagatatata
     2341 agtttaggaa agatatttga tgatagttca tatgaatatt atagtaattt aaattatttg
     2401 aaagcctcaa taggtagtga aaaagcagat atgaatgcat ggattgagta ttttttagag
     2461 acgatattaa ctgcatatga gaagctggat gatagtttaa atatatctga taagaaaaga
     2521 caaacaaaaa ctagtagaat agagaaaata ataaattcta cccttggata ttttacaaaa
     2581 gaagatataa gagatttatg tcctgacatt ccagaaccta ctataaatag agtttttaat
     2641 aatctaagaa aacaagataa gatagaagtt gttgcaagag gaagaagtgc aaaatggaag
     2701 aaaaaatatt aaaaaatgtt tagataacta aaatggtata gtgttttaca ctataccatt
     2761 tttaatgtca ttagcatgag caataattta acaacttaag caatatcaat tttatttatt
     2821 gattattttt gaactgaaaa atggcgttat atggtatacc tcagtggtaa ttaattttgg
     2881 atgagaagat ataatattac agaatatatt aggagtatat gtagaaaaaa tataataaaa
     2941 gtgtgaggct tagtaattaa caaattatgt ggcttcacac ttttttataa aataaagata
     3001 cttagcagaa taatatttta gttgatatgt ttataagatg gtacttgttt gggtaaaagt
     3061 ataaatagta aaaaagtaga agtaatgtgt ataaaaacat aagttacatt ctgtaataat
     3121 agttatttta taattcaaca aaagaataga atgggtatat ttgtgaaata ataatataaa
     3181 ataatcttag gagtgagctt atgaagaaaa aattattata tataaatgta aattcaaaac
     3241 ctgaagactt atcttcaagt aaaactgtag caagaaaatt tataaataaa tttatggaaa
     3301 aaaataaaga ttttgaggtt gaagagatag acctttataa agaacatata ccaagacttg
     3361 agtatcagta ctttgaaaaa agaaatagca tagttagtga agaaaatgct aaaaatttag
     3421 acgataagga tagaaaagag cttacaaaga ttagaaatct ttgtgaccaa tttgtgaatg
     3481 caagtgttta tgtaatagca gcacctatgt ggagtttatc ttttccagca ccacttaaag
     3541 agtatataga ttgtataatt caagatggaa aaactatatc ttttgaaggc aatgataaac
     3601 cacaaggaat tttaaatgat attgatagaa gtatggtata tatacaatct tctggtggac
     3661 acataccatg ggtgttgaag ccagttatga ataaagggtt aaattatgtg gaaagtatta
     3721 tgaaatttat aggaattaaa aaatttgatg aattgttagt agatggaact ggaactagtg
     3781 aagaagaaag acaagctgca atagaaaaag catctgaaaa aatagatggt ataatagatg
     3841 ggatgaaatt ttaattataa tattttatta aaaatataaa ggaaaaacct cttttaatgt
     3901 agaaacttat attgaaggag gcgagaatca tgcgagaaga aaaaagtaat gaaaagtatg
     3961 attgttattg gtgtaatcaa gagaataatt tctgtgtaga aataaaagat aatatagtca
     4021 tgatagatga tggcactggt acgttaaaac aagccgtttt catagggtat aaacaaatcc
     4081 aaattaattt aaactgttca cattgtcaaa acttaaatag aataaaatta aatttgtaga
     4141 attttaaaat ggaaattatt gaaggaatta aaaatagaaa gtgttaatat ttagactgaa
     4201 aattatattt tatacaataa aagtttattt aaataacatt gtattttaat atttattttc
     4261 agtcttactt tattaaaatt attttttggt tatgtctgga aaatatctgt ctactaattt
     4321 ttctgaaata ttttttaatt ggtcagccat tgaagtcaga cttaataagg ctatcaataa
     4381 atcatcttta ttttctggat ttatactaat attttcaaaa ttagactcta taaagtccgg
     4441 tatattcttt ctttgatttt cttttattaa catgaatttt tcgtaacatg aaactaaatc
     4501 tttttcactg aaattatcct tttcatacac accatgtaag attcttttaa tttcttgtat
     4561 tgtaagtgta tttttcatag aataaataat tatcatctgt aaaatttgtt ctttagtata
     4621 cttttttccc ttaactggtt tgattaatcc ctctttgctg taattattta tcatggtttt
     4681 agtaagaatt ttatcacttt caaatctttt attagcagaa agcttattgt caattagaga
     4741 aataatttga tccatatata agtcaataga tggaatatcg tttgaagtta tatctgcatt
     4801 atttagtgtt tctagtatta tagtattaag ttcattattc atcatatgtt acctccataa
     4861 tatatataat acactaaatt agaaagtaaa acaatattaa ggcttgtaaa aaaaacataa
     4921 agtggtataa ttaaatatat aatatagtaa tcaaaactat atttatgaat ctgattttta
     4981 aaatacataa ttgtaggagg acgaaatgta tcaatttttt ttaaagggta gagacccaat
     5041 aagcagttta actcatttta taggtgcttg tttatctctt ttagcaacta taattttagt
     5101 ttttcaatct gttacattac aggaaacttc tctcttgatg atagtatctg tatctgtatt
     5161 tggactttca ctaattgctt tatatagtgc aagttcgtat tatcattttt taaaggggac
     5221 accagaacaa gaattgtttt ttagaaaagt agaccatgca atgatttacg tattgattgc
     5281 tggctcttat acaccaatat gtctgaattt tatggaaaaa aaagaaggca taatatttgt
     5341 tacagcaata tggatagttg catttattgg cataataata aaaatatttt ggatggatgc
     5401 acctagatgg ttatctacca gtatatattt acttatgggg tgggcaattg tttttgatat
     5461 aaatgcattc aattcaattc ctaaggattg ccttagacta ttaattatgg aaggtgtatc
     5521 ttactctata ggtgcgatta tttatataat taaaaagcct aatataagtc ctgagtttgg
     5581 atttcatgaa atatttcata tttttattat gataggttct ttatttcact ttttagcagt
     5641 tttattgtat gttttatagt tttaaaagat ttgattaatt gatactaaaa ttctacaatg
     5701 aataaaatgg aactgcctta tatagattat atcttagaca ataagacagt tccttattat
     5761 aaatattaaa ttctacttat aataaaattt tgataaaaat agtccattca aaatgcctat
     5821 tccataattc ctttaatgta cttttcattt acgacatata gtttgaattt atgtactata
     5881 attatatcct ctaaattctc tgaaacacta acaattctga cacatttgtt tctaatttct
     5941 acaggtaaat ctaaatcatt tgttaaattc acatagtcat catatttata cataaaatca
     6001 tccctttaca ttaagatata cttattatac tatattttaa taagtttttt taaattatta
     6061 caatttttcc cgaaatttca ttttttatac ataaaat
//
LOCUS       DAELNY010000206         6073 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.209, whole genome shotgun sequence.
ACCESSION   DAELNY010000206 DAELNY010000000
VERSION     DAELNY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.209"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..754)
                     /locus_tag="KPE55_003276"
     CDS             complement(<1..754)
                     /locus_tag="KPE55_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494315.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFD"
     gene            complement(996..3008)
                     /locus_tag="KPE55_003277"
     CDS             complement(996..3008)
                     /locus_tag="KPE55_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0494316.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGITKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            complement(3513..5531)
                     /locus_tag="KPE55_003278"
     CDS             complement(3513..5531)
                     /locus_tag="KPE55_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0494317.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            complement(5805..5921)
                     /gene="rrf"
                     /locus_tag="KPE55_003279"
     rRNA            complement(5805..5921)
                     /gene="rrf"
                     /locus_tag="KPE55_003279"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5930..6003)
                     /locus_tag="KPE55_003280"
     tRNA            complement(5930..6003)
                     /locus_tag="KPE55_003280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5969..5971),aa:Gly,seq:tcc)
BASE COUNT         1845 a         1007 c          718 g         2503 t
ORIGIN      
        1 aatcaaaata tatataactt tgtaatgtat tatccaatgc ttctgttata aaatcaactt
       61 ctgcattact tgagctttca aggctatcca caattatata tctcacttct gtaaataagt
      121 tttttaaata aaaatcatca ttaagtaggt attgattgta taaatatatg cttaatgcat
      181 tatctaaaat tgaatttgat agtaaggaat ttatataata tgatataatt tcatccattt
      241 gagtgtatga aaatctatat atactatctt tatttttctt tgatgaatat attttttctc
      301 caataaaatc aaaatcaatt aaattaaatg cagacttatt tatgttagtc attatactat
      361 ttgctatgga tttattggtt cctgtaatat cttcaaaata tccttcttgt atcctttttt
      421 ccttaacttt ttgcactatt aaatattcac ttaaactatt tgatataaat gttggagata
      481 caacctttgt acttatttca tcacattttt ctattattag tggccagtat ttaactaatt
      541 ctttcttcac aaaacttata tatgttgtta ttttcagttc ttcactataa tttaatctta
      601 gagacctttc atatcgtatc tttgtgttat tgtttggtac taacaaaaga atacaccttg
      661 aattattacc agttttatct aataattctc tatatttatc taaagcttca tttccatatt
      721 cttttaaact acttttttca aataaatatt ccatataatc accttcctca attaaattaa
      781 gtgtatatta tctaattata tcatattgtg tattttacaa aacacaatca aattattgca
      841 tttaactaca aaaaaagact gtatatatac ttgcattcac atgtgcaaat gtacatacag
      901 tcttactatt gatacactta tatttcattt tttgacttta gtataaattt acaactataa
      961 atttacatta agtttttaaa tatttttatt tccatttatt ttaatgaatc ttctattcct
     1021 ttacctattt gtgtacccat ttcttttact tttgcagatg agtttattaa actcatatca
     1081 ttttcattat ccaaaaatgc acattctaca agtacagctg gcatatcatt acttcttaaa
     1141 acgtataaat aatcttttgt agaatcacta ggaagtactc ttgtttttat acctctattt
     1201 gtaagtttaa atttctctaa aattctattt aatatattgg tagctacagt tttagtaatc
     1261 cctccatttt tatctttaag cttataaaat acttctacac catgaccttg tttattagtt
     1321 gagccattat aatgtatact tgtaaataaa tctggtttca atgaattaga tagagcagtt
     1381 ctatttccaa gagataaagt cttatctgtg tctcttgtca ttattacatt gaatcccttt
     1441 gaacgtaaat attcagttgt tgcaagtgaa gtatttaaat tgtaatcttt ttcaatcatt
     1501 cctccagatg aatttccagg tgctgaacca ccatgccctg ggtcaatcat aactgtcttg
     1561 ccaccactat ttcctggttc tgttggagtc gtattgtgtt tagataatga tgatgctata
     1621 gagctcatta ccttagaagt caatcctcct cctattttgt ataccttatt agctgattta
     1681 gcttctaaat tatctttatg gtatgcagat acatacgtct ttggtgttat aagtattgga
     1741 gattttaagt tcgcagccaa tggacctgct gctaaagcat caacaagtac atctgattta
     1801 gctactaaaa cagcctctaa ctcatcatct gtatagaatt tttttattac gtttgcattt
     1861 gtttcgtgtc tatctgctcc gtatactcta ttattagtaa cactatcttt agttattcca
     1921 tttaccttat ttataacatt tgttgatatc atttgaggac cacctataaa ataagcattt
     1981 tgtaaatcct cactctttaa ccatttatat gtattgtctg taatactatc tttatcagtt
     2041 aatataattg gttgcttgtc ttgacctgct tttgctgcta tagtaagtgc atccacttct
     2101 ccgccattag catttgttat gtatactttt tccacatcat gatttttatc tatttccttt
     2161 gctatcaata aagatgtctc atatctatta gaaccattta aacgtgtttg actagcattt
     2221 acagttgatt taatttgatt agcagtagtt ttagaaatag cattctcatc tccaattata
     2281 attatatctt tagggtttag gcgctttaat tctgatttta cactattagg tacattgttt
     2341 ttttcaacca aaagtattgg tgcattatat gtggttgcca gtggagttga tataatgcca
     2401 tctatactta catccccatt tattataact accttatctg aaccattttt ccagccctct
     2461 ttacttattt taactgctgt ttcatatcta tcacttcctg taagttctgt aataggtgaa
     2521 gaagtttttc tcaaatattt atttacatct tttctagaag ttatatcttc aattatacat
     2581 tcctgtgtta cattatagtc ttttaaattt aatcccagct tttctaggtc ttgtctgttt
     2641 aaagttttag gattattttt gctgtttatt gttatccctt gtagttccca aacatttaga
     2701 tattctgggt cttggttata attgatagtt ccttcatatg ccccagttga ttgattgtaa
     2761 tttagagttg tattcaatgg catgtcatag catttatact ttaatgtaat gctatctgca
     2821 tctggttttt ctttaaattt gacactaact tttgatttgc tacctaaaac gatacttttt
     2881 ttctctaagt ttatatttaa atcagcgtta ttttccatag cattagattc agtagcaaat
     2941 gccattgtag gtgcgacgat tgccattgaa agaatccctg ttgttaataa ttttgtattt
     3001 cttctcatat taatctcctt acttaacata aatttctact ttatttttaa tcttttaact
     3061 tttgaaaata tgtcaaagtg tatctaaccc aaaatataaa taatatataa gtaatatata
     3121 aaaataaact tataaaactc aaaaaataaa aaaattaaat atatttaata catttaatta
     3181 tctatactat atcatttatt gacataaaaa tccacaaaaa acacacaaac acacaataaa
     3241 atatagtaat taagctttta ttgtgtatta caaatgaata ctccaactct tgacaattaa
     3301 ctgtagcctt aagatatgta aattcttcat ataatataaa tccttttaca attaatcata
     3361 aaaagactgt atatacctca tttcgcactt atatgcacaa tgtatataca gtctttatta
     3421 ctattataat tacattctta tcttatagct ttagtttaaa tttaaaattg cagattaatt
     3481 tattttaaat ttttctatgc tctcattgta gactatttta atgaatcttc tatgccttta
     3541 cctatttgtg tacccatttc ttttacttta tttgatgtat taagtaaact catatctttc
     3601 tcattatcca aaaatgcaca ttctacaagt acagctggca tatcattatt tcttaaaaca
     3661 tataaataat cttttgtagg gtcagttgat agtgttcttg tttttgcacc tctatttttt
     3721 agattaaatt tctctaagat tctatttaat atatttgtag ctacagtttt agtagtccct
     3781 ccatctttat ctttaagctt ataaaatact tctacaccat tcccagttgt atcagatgca
     3841 ttatagtgta tactcgtaaa taaatctggt ctcaaagagt tagataaagc agttctattt
     3901 ccaagagata aagtcttatc tgtgtctctt gtcattatta cattgaatcc ctttgaacgt
     3961 aaatattcag ttgtcgcaag tgaagtattt aatgtatagt ctttttctct aataccccct
     4021 aatggtttac ctgttgttcc agtgtccgaa ccaccatgtc ctgggtcaat cataactgtc
     4081 ttgcccccac tatttcctgg ttctgttgga gttgtattgt gtttagataa tgatgatgct
     4141 atagagctca ttaccttaga agtcaatcct ccacctattt tatatacctt atttgccgat
     4201 ttagcttcta agttttcttt atggtatgca gatacatatg tctttggtgt tataagtatt
     4261 ggagatttta agttcgcagc caatggacct gctgctaaag catcaacaag tacatctgat
     4321 ttagctacta aaacagcctc taactcatca tctgtataga atttttttat tacgtttgca
     4381 tttgtttcgt gtctatctgc tccatatact ctattattag taacattatc tttagttata
     4441 tcatttacct tatttataac atttgttgat atcatttgag ggccacctat aaaataagca
     4501 ttttgtaaat cctcactctt taaccattta tatgtgttgt ctgtaatact atttttatca
     4561 gttaatataa ttggttgctt gtcttgacct gcttttgctg ctatagtaag tgcatccact
     4621 tctccgccat tagcatttgt tatgtatact ttttccacat catgattttt atctatttcc
     4681 tttgctatca ataaagatgt ctcatatcta ttagagccct ttagacgtgt ttgactagca
     4741 tttacagtcg atttaatttg attagcagta gttttagaaa tagcattctc atctccaatt
     4801 ataattacat ctctaggatt taaacgcttt aattctgatt ttacactatt aggtacattg
     4861 tttttttcaa ccaaaagtat tggcgcatta tatgtagttg ctagtggagt tgatataatg
     4921 ccatctatac ttacatcccc atttattata actaccttat ctgaaccatt tttccagcct
     4981 tctttactta ttttaactgc tgtttcgtat ctatcacttc ctgtaagttc tgtaataggt
     5041 gcagaagttt ttcttaaata tttatttaca tcttttctag aagttatatc ttcaattata
     5101 cattcctgcg ttacattata gtcttttaaa tttaatccca tcttttctag ttcttgtttg
     5161 tttaaagttt taggattatt tttgctgttt attgttatcc cttgtagttc ccaaacattt
     5221 agatattctg ggtctttatt ataattgata gttccttcat atgactcagt tgattgatta
     5281 tagtttaaag ttgtatccaa tggcatatca tagcatttat actttaatgt aatgctatct
     5341 gcatctggtt tttctttaaa cttaacactg acttttgagg tactacctaa aacgatactt
     5401 tttttctcta agtttatatt taaatcagcg ttattttcca tagcattaga ttcagtagca
     5461 aatgccattg taggtgcgac gattgccatt gaaagaatcc ctgttgttaa taattttgta
     5521 tttcttctca tattaatctc cttatttaac ataaatttct actttgtttt ttaacctttt
     5581 aatttttgaa attatatcaa agtgttttta cccaaaatta taaataagta cataaaaaat
     5641 aaacttctaa aaaaattata aaaacaaaaa ataaaaaaat taaatatatt gaatatattt
     5701 aattatcttc actatatcat ttattgccaa aaaaatctat aaaagacaaa taaaaacata
     5761 taacaattgt aggtttatta aatttaatac aacaaaaaaa gattacgtgg ctacgtccta
     5821 ctctcccagg cagtttccca ccaagtacca tcagcgctag agagcttaac ttctgtgttc
     5881 ggaatgggaa caggtgtatc ctctctgcta ttgtaaccac ataatctttt ggagcgggtg
     5941 aaggggatcg aaccctcaca gctggcttgg aaggccagaa ctctaccatt gagctacacc
     6001 cgcattaaat ttttataaat tagtaaaact aattttttag agttagcact ctaaaaacta
     6061 catatatatc cta
//
LOCUS       DAELNY010000207         6044 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.210, whole genome shotgun sequence.
ACCESSION   DAELNY010000207 DAELNY010000000
VERSION     DAELNY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.210"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            171..824
                     /locus_tag="KPE55_003281"
     CDS             171..824
                     /locus_tag="KPE55_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0494318.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            965..2236
                     /gene="brnQ"
                     /locus_tag="KPE55_003282"
     CDS             965..2236
                     /gene="brnQ"
                     /locus_tag="KPE55_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0494319.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2438..3625
                     /locus_tag="KPE55_003283"
     CDS             2438..3625
                     /locus_tag="KPE55_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG0494320.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3855..5879)
                     /locus_tag="KPE55_003284"
     CDS             complement(3855..5879)
                     /locus_tag="KPE55_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0494321.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         2129 a          712 c          944 g         2259 t
ORIGIN      
        1 ttttatacta ttaatggaag tttattataa atgagtttgt gatataatta taaaagtact
       61 gaatacatgg atatttcgta aaaacttttt aatatatcaa atatgtatat taagttaata
      121 tattaaaaaa aataatatca aaaataatat aatttatagt ggaggggaaa atgactaaaa
      181 ttattaaagt gcaaaaacct aaatttacag gagaattaga aattattgaa aatcttgaag
      241 atatacttga agatatattt aatgatgaaa aaatatttaa taaaattata gaaaatgaag
      301 ttataagtga tttagattct gtaagagtta gctttgaatc ttgtatattt agaaatgtgt
      361 cttttgaaaa ttgtaattta aaaaagattg atttacttga tgtggtattt gaaaactgca
      421 atttatcaaa tacatttttt gatggaggaa gtatatacag agttgaattt aaaaatagta
      481 aattgatagg agttagattt gatgattgta tacttaaaaa cgttttgttc aaggatgttt
      541 taggtaagta ttctaacttt tcattctcta aatttaagat agtttctatc ttagattcaa
      601 attttgaaaa ttctgtattt caagaagtaa aaggtgacaa tctagtattt gagtatacaa
      661 atttaaaaaa agcaaacttt aatggaacta aactttctgg catagatttt actactaata
      721 atattgaggg aattgagatt ggtatagatg atattagagg agcaattttt gatgtgtcac
      781 aagcaattga tttaacgaaa ttgatgggaa ttatcataaa atgaaaaaaa tattcataaa
      841 ataaattggg aaaacgttaa caatgtgtga atttttacca gattgacatt tttctgaaaa
      901 taaagtaaga ttatcttaat attcaaaata ttttcaaagg gggtaattta gtgtgagtaa
      961 gcagatgaga gatattttta tagttggttt tgctttattt tctatgttct ttggtgcagg
     1021 aaatcttatg tttcctccat ttttagggat ggaatccggc aaagactggt taattcctct
     1081 tattggattt gttgttgcag atgctggtat agcaatgctt gttattatag caacagcaag
     1141 atgtaaggga agcatggatg atgtacttag gagagctggt aaagggttag caagagtaat
     1201 gagtgtagct gctctagcat gtttagcact tcttgtaata ccaagaactt gcgcaacaac
     1261 atatgaaatg ggtattatgc ctatatttgg agaccatggt ccagtagcta aagctgtatt
     1321 ttcaataatc ttctttgggc taactttagc atttactatt aggtcttcta aggttataga
     1381 cataataggt aagtatttaa ctccagcgtt attattagta ttatttgttt taatagtaaa
     1441 gggtataatt tctccagcag gacctatgag tcctgagcat atgatagata aaaatctatt
     1501 tggtgaaggt atatcacaag gttatcaaac aatggacgct ctaggtgcag catctatggc
     1561 aactataatg ttgatgtcta taatagctaa aggatataca tcagataaag accaaatagg
     1621 aatgactatt aaagctggtt ttgtagcctg tgtatttcta gcagtagtat atggtggtct
     1681 tacttattta ggtgcaacag tttcaacttt atatgatact agtatacctc aagcatcttt
     1741 attagttgaa ataactagta tattgttata tggtcaagta ggaaaggtaa ttttaggtgt
     1801 aattgtggca cttgcctgtc tgactacttc tacaggttta acagcatcaa tatcaactta
     1861 ttttgaagga ataagtaagg cgagttataa acaactggtt ataggtatat gtgtagctag
     1921 tatgcttata tcaaatttag gtgttgattc tataatagcc atatcagttc caatcctaca
     1981 aactatatat cctgttttat tagcaatagt tgttatggag ttggctggta aacatataaa
     2041 gaatgataat gcttttaaag gtgcagcata tgttacatta gtgattagtt tattatcagc
     2101 tataaatgga atgacaggag ctgttccatt tatacaaagc ttgccacttg caggtttggg
     2161 ctttaactgg gtaataccag ctatattggg tggaatagtt ggtaacttta taaaaagcaa
     2221 caaacaagta gcataataat ttgtaattaa tttaatttta taatatatta atttttagta
     2281 ctatgggtag gtttaatcta aacctaatct acccatagta ctttttagat tggattataa
     2341 tagcatgtat ataatttttt ataatataat ttttctttga agtatgataa aattagtaat
     2401 agtgataaga atgtacaaat ttaggaggtt ttaattaatg cttagaaaaa taacagcatt
     2461 tatgctatcc atatgtttaa ttattggact tacaggttgt agtttacttg taggcaagaa
     2521 agataaagag aaaattatag atgattttaa agttgcagtg gtaactcaac cattatctga
     2581 aaataaagta caatacaata tggtggaaga gatggcaaaa gaatatgagg aagagaataa
     2641 aattgataaa gacaaagatg gtcaaactaa agttaaacaa actataaagc atgttgtatt
     2701 gccagagaat tttacttcta atatagacag tgctattaat aagatagtaa agttggctga
     2761 cgataaagaa gttcaggcta ttgtagtttc aactgaccaa gcagggcttt tacctgcttt
     2821 acaaaaggta aaggaaaagc gtccagaaat aataactatt tctgccccta tgggagatga
     2881 taaaaaccaa ttaagccaat ttgttgatgt gaatctaggg gttagtgcag aggaaagagg
     2941 aaaagtatta gctgaacgtt caaaagaaat gggagcaaaa gcttttattc attatgcctc
     3001 tacagatgat ttgaaagatg ttaatatagc caaaagatta gaaatgatta aagagacttg
     3061 caaaaatata ggattgccat ttgtacaagt taatactcca aatataaata ctgaagaaga
     3121 caaaaacaaa gttaagcagt ttttaaatga agatattgaa aaacaagtta aaaaatatgg
     3181 aaaagatata aatgtatttg gtgtaaatga gtatatggat gaagttatat tgactaaggc
     3241 tttagaatta aagtatatag ttgcagagca aagcaatcca tcaccaatac aaacatatcc
     3301 tagtgttatg ggtctaaaaa tatctgaaaa agatgctcaa aattatgata aaataaacga
     3361 catgataagt gaaaaggcta aggcttttgg catgtcaaat agacttggtg gatatcctat
     3421 gccaatggat gcttttctac catcagtagc aatttatctt gctactgaaa tggtgaaaca
     3481 agatttaaca caagaagatg tatgtgaccc agattattta gaggcgttta cagaattaag
     3541 atttggaatc ggttcagaat ttactccttt aacagaggta ctttataatt atcaatcagt
     3601 aattttaagc caattaattt attaatatta aggaagtgtc tcaaaatgaa ctttttagtt
     3661 tatgaggcac tctttttcta ttaaatcaaa tatattttta tctttttaac aattaaaaag
     3721 aagcttatcc ctatattgag atagcttcgt ttttaatata aataagtttt attattttat
     3781 aaaaaattta tagaaaaata aaaaattata attatatata aattttgata aagtaaagat
     3841 gaattactat aggtttaaat tatattattt tctattattt ttatgccttt ctcagttatt
     3901 gatgagcctt ttcttccttt actagaaatt aagtatccat tgcgtgtcaa atattctagc
     3961 atatttctta cttcgtattc tgtgacatta tattccataa atttcattaa ctttgtcctc
     4021 cccattggtt tcatgtgcaa tgattttagg atagaaatgc atatgttttt gtcatattgg
     4081 tttaaatcat tgaatatgga catattactt gacatatgat tattatttaa ttttgaagat
     4141 atgttttgag gtaaatcatt tattgtaaga taattggaac ccatgtaagc catatattca
     4201 atacaatttc tcagttctct aatattcccc atccaagtgt atgaatttaa agcttctaaa
     4261 agactcttat cgagttttat gtttttatag ggaagttctt ttaaaaatga ttctaaaaga
     4321 agaggtatat cttctaatct ctttctaaga ggaggtaaat ctaaagtgaa catacttatc
     4381 ctataaaata aatccatcct aaatttattt tctataatta atttctctaa atctttattt
     4441 gtggcagcta atattctcac atcaattgga ataatacttg taccaccaat ttttctaatt
     4501 tctttttctt gtaagactct taaaagttta acctgtaaat catgtggcat atctcctatc
     4561 tcatctaaaa atatagtgcc agtgtgagct aattcgaata atcctttttt tccacctttt
     4621 tttgctccag taaaagaacc atcttcatat ccaaatagtt cactttcaag taattcactt
     4681 ggtaatgaag cacaatttat tgctacaaaa ggtttatttt ttctatttga atgattgtgt
     4741 attgactgag taaaggcttc tttacctgtt cctgtttcac ctgttattaa tataggatta
     4801 tctgtaagag ccattttttt tgccttttta atacaatctt ttataataga actatttcca
     4861 ataatattat taaatttata tttagctaca taacctcttt tgacagaatc acttcttatt
     4921 ttttgttcta atatttctat attgtttaca tctttcaatg taattaagca accctctata
     4981 tttttataaa gataaaatgg ttttttagaa agtatgaatt ttttatctat ttcgtctatg
     5041 tatataatca tattatcgat atttaagttc tttgtaattt tatctaagaa actatttggc
     5101 atatattcca ttaaggaagg tgattttata agtttttcat ctagtcccaa catattaagt
     5161 aaagacttat ttacaaaagt aaccttgtta aaggtgttga aaaatattag accatcacct
     5221 atttcatcaa tggtcatgta aagtatagtt tttaatttag atatattatc taatgatgta
     5281 tttaagaaat catgacttag tgtctctttg gacaatttgt atagtttttc tataaatttc
     5341 tcatttttta gttttaatac tactaataat gacatatatg tatctaatga gatttttctc
     5401 caacctaaat caacaatatt ttttatgtat tttggtataa atctaatctg acctggtgtt
     5461 atagctgtat ctaaatcata tatttcatca caatctggat atgtcggtac aaaatctata
     5521 tgatttatac caattttata tagataagta gccatctcaa atgctgaagt tttattggtt
     5581 gtcataagta acgattttgt acctggaggt agtttcatca ttttttcata tccaagtttt
     5641 gaaagtgctt tcgttccatg aataatttca atatcttcct taaaatgttt tattaaaaaa
     5701 ttactagtta aaggtgatag agaaagcact acatctccct ctaaattttc ttttattcca
     5761 tcaagtatat tttgtgtttc tatgattatt tcgtctccaa aaaagtttgt taattcttct
     5821 ttgtagtggt cactattcaa tttatcatgt gttataagga ttaacttctt tttatccatt
     5881 ttatttctcc taacaaaata gtatttattt ttaaagtatt taaagtataa ggcaagttat
     5941 attatacaac aaatagtagt ataaatttat atatttgatt aaataatagg tttttttagg
     6001 attttatcct ataaaaacct attttaaaat tatttttgca agat
//
LOCUS       DAELNY010000208         6020 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.211, whole genome shotgun sequence.
ACCESSION   DAELNY010000208 DAELNY010000000
VERSION     DAELNY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.211"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            239..1051
                     /gene="modA"
                     /locus_tag="KPE55_003285"
     CDS             239..1051
                     /gene="modA"
                     /locus_tag="KPE55_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0494322.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1061..1852
                     /locus_tag="KPE55_003286"
     CDS             1061..1852
                     /locus_tag="KPE55_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494323.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1854..2549
                     /locus_tag="KPE55_003287"
     CDS             1854..2549
                     /locus_tag="KPE55_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0494324.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMSVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2536..3267
                     /locus_tag="KPE55_003288"
     CDS             2536..3267
                     /locus_tag="KPE55_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494325.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLERLCFCSK"
     gene            3581..4381
                     /locus_tag="KPE55_003289"
     CDS             3581..4381
                     /locus_tag="KPE55_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0494326.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4921..5637
                     /locus_tag="KPE55_003290"
     CDS             4921..5637
                     /locus_tag="KPE55_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494327.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2345 a          723 c          851 g         2101 t
ORIGIN      
        1 atctacaata agttaatttg atttaagttt ttaattttta ttaatacttt aaagtattaa
       61 taaaaattaa aaataaaata actacctatg gaaatgacat tataaataga ttttttctta
      121 actatacata acaaaacata acaaaacatt ataaaacata acaaaaactt agacatcgtt
      181 ttcttagtat gatatactaa aaaagaattt acactaattt actaaggggg aatatcttat
      241 gtttaaaaaa ctttcagtac tagctttagc tatgattatt tcattaagta ttactgcatg
      301 taattctaaa gaaaacaaag ctaatgatga atctaatact aaagtgacaa cagctgatga
      361 ttcaaaagaa aaagagactc tttttgtcta ttcaggggca ggtttaaaga aaccaatgga
      421 tgaaatagca aaaaaatttg agaaagaaaa taatgtcaaa gttgaatata gctatgcagg
      481 ttctgcacag ctaatagctc aaattgagac aagtcacaaa ggagacgtat ttattgttgg
      541 ttctgaacca atttattcaa aggcctttga aaaagaactt gtaggtgaac ataaaacggt
      601 tgcacatcat acacctgcaa tagttgtacc taaaggaaat ccagctaata ttcaaaaatt
      661 ggaagactta aataaagatg gagttaaact tattttggga gacaaagagt ctaatgctat
      721 aggtaagact acacaaaaaa tacttgaaaa aaataattta aaatctataa atgataatgt
      781 tgtctctaca gctgcaactg taaatgaaat gattgtacag ttaacatcaa gtaaagctga
      841 tgctacaata gccacaaaag attcggtctt tggtaatgaa gatgtagaag ttatagaaat
      901 tgcccaagat aaaaatatag accaacttat atcagcagga atcgtaaact atagtgataa
      961 aaaagattta gctaataaat ttataaatta tatagagtca gacgaagcta aacagatatt
     1021 taaaaaatat ggttttgaac ctgttaagta gtgattaaac ttgaaaaata aattttttac
     1081 tactctactt actggatttt ttgctatagt ctttttgttt attgtatcac cacttcttat
     1141 gttaatcatt aaagggataa gttatgttcc tatatgctta aaatcagtgg aagtacagta
     1201 tgcaataaca cttagtatta aaacatcttt aatatctact atagtatgcc ttttattagc
     1261 tattccagta gcatattttt tacatattac taaattacca tttaaaaaac tgattataca
     1321 gataataaat ctccctatgt ctcttccaca cttagtatca ggaattgctc ttttactttt
     1381 gtttggaaga atggggattg gagattccat ttataagatt tttaagcttg attttatatt
     1441 tacaaagcaa ggtatagttt tagcacaaat atttgtaaat cttccactta ctattaaaat
     1501 acttcacact tcattaaatg aaagtaatga aaagatgata tttgttgcac gtacccttgg
     1561 atgtaatagt tgggaagcat ttagatttat aattcttcca aatctaaaaa ctggaatcat
     1621 atctgcaact gtaatgactt ggtcaagagc acttggtgaa tttggtgcag tagctatgat
     1681 agcaggttct acacgtatga aaactgaaat tattcctaca tctatatatt taaatatgtc
     1741 tactggtgat atagacattg caatcggaat agctgtaatt ttgatattta tctcacttac
     1801 atgcttgatg ctttttgaga tattttttaa tagagaggtt gataagaatt aatatgttaa
     1861 aaataaaaaa tttatctctt gatttaccta attttaaatt acaagaaaat agtttaaata
     1921 ttaataatca tgaatacttt gttttattag gagctagtgg gtcaggtaaa actctattcc
     1981 tagaaacatt agcaggaaga tatccatctg ctaaaggaaa tgtatcatac aaagaaaatt
     2041 taatatcaaa cttacctcca gaaaatagaa atataggatt tgtatatcaa aattttgaat
     2101 tatttccaaa tatgagtgtt gaagataata taaggtttcc acttaaatta agaaaaatac
     2161 ctatagatga acagattctt aaggctaacc acttgtctaa attaatgcag atagaagata
     2221 taaaaaatag gtatcctaaa aatttaagtg gaggcgaaaa gcaacgagtt gcttttgcac
     2281 gtgccttaat agctgaacct gatatattac ttttagatga acctatgagt tcattggatt
     2341 atattacaaa aaaacatata agcaaaattt tgaaggatgt ctacataaaa tacaaaccaa
     2401 cagtgattca tgttactcat gatatttctg aagcaatgtt ttttgctcat aagataggaa
     2461 taatgaacaa tggaaaaata aatcatattt ttgatttaaa tgaagaaata aaaaataaag
     2521 gagaatcttt tttctatgaa tatatttgat attttgagag attatctatt agtacaagtc
     2581 gataaatata atttaaatat ggatatgata agtattgtgt ccaaatctct atcttcaaaa
     2641 gaagcaattg gaaatactaa gcgtaaagat tttccaatta tagttggtaa agaaataatg
     2701 cttgaagctg actttaaagg agctaaagga caagcattta cttctactcc atctacattt
     2761 gaaggttctt taaaagacat attatcatta gatttacatg acaatcctca tgatagaagt
     2821 ctttttatag catcattaaa tgcagttatg aaatacttag gcaagacgga tagaaccatt
     2881 cattgtaaaa ataacgagcc agaggtatgt gcaaaaaaat tccctgagtt tataaaaatg
     2941 gaatttggta atccaaaagt agcaattata ggatatcaac cagccataat tgataatatt
     3001 aaggactttt tcgaaacaag agtattagat ttaaatcctg aatttgtgga tactattcaa
     3061 tataatgtta agattgaaga tggtatacga gattatgaag atgtaatatc ttgggctgat
     3121 ttagtgattt gcacaggaag tacattatgt aataactcaa ttataaattt tctatcactt
     3181 aacaaaccag tttactatta tggaactacg atagctggtg catcaaatat tttaggttta
     3241 gaaagactct gtttttgtag taaataagca atcatatcat caaggagtac acaaatatat
     3301 atgtgtactc tttaaattta aaaagttatt acttttattc taagtactaa agtaaaattc
     3361 tattaaaatg aatcccgaat aaatgtacaa tccctttaaa tctcaattcc tatcaatcat
     3421 taatattttg attagtctta tacataaatg ttttctttat tttaaactaa taaaaaaatt
     3481 ttattccatt aatctactct atatttttca gcatttaaga aataattttt agtactttgt
     3541 ttgataatat aactaagaca acttttcagg agatgattaa atgacattta agaataaaaa
     3601 atttactata aataaatata aaattctatc tattattcta gttttatgtt tgattttaac
     3661 cgcttttagt agctatgtga attatagtca taaaaaattt aaagattatg ttgtcttcta
     3721 tgttcagcat caagatgatg aagtgttgtg ggctgggagt gctattgtta atgctataaa
     3781 agaaagagga aaaaatcatg tatttgtggt tttagtatct actggatgtg gtatatccgt
     3841 ctttgataaa tataaaaaat atgaaaacct aacaaatata caaaaatcag aatatagaaa
     3901 tagagaattt ttagcttctc ttaatagcct tggtgtaata agggaaaata taatattatt
     3961 accagaaaca aatacaaatg gtaatactga cttcaattac atggaaaaaa ttgctcttac
     4021 atttgaagaa aaatttaaaa atgtaactca tatagcacat acttataagt tagatgacca
     4081 tttacaacat ttaaaaaatg gttctgttat ccaaaattta tataatgctg gtaaaataaa
     4141 agatgctaaa tattttgtaa aacctaaatt tgctgataaa ataactttta ataataagat
     4201 agtttataaa gctcattcta ctgaagacta tgaaaaggtt aaaaatgcct gtcaacaata
     4261 caaaattgtt gatgaattag aatatagaga aggtattggc tataagtctg accataaatc
     4321 atttgataag ttattaaata ataaagatgt tcctgctata cttcatacac ctaatttata
     4381 agaaatattt attcttttaa tttaagttat aaaaaggaaa ttatttgaga ttttgtgata
     4441 tagcactttt tgtgctatac tactttttct catacaacta ttacccatat ctaatttcat
     4501 catctcttaa aatatataaa gatatcatca ttaaaaccta taagccttat taaatgcttt
     4561 atacatacct acattaaact acctgtttat attaaactac attaaaataa atatttgtta
     4621 taaattctta ataattatga aactcatttg ttatttatat ttgttaatat atgtttaaac
     4681 ctaaaaaatt aataaaccaa aataacataa gaagtgtgat agattaccct agatattttc
     4741 ataaactttt accttcagat taatatgggc gtctatactt ttaaatttac tatatgcatt
     4801 gacaatatat taaaagtact agtttttatt tcataatgaa cgggcttact gttttctaat
     4861 acctcttttt ggtataaaat aaaaattggt taaaaataaa atttcaaggg ggttaataag
     4921 ttgaatcaaa atatattggt tgtagatgat gatagagaga tagttgatgc tatagagttc
     4981 tatttgagac ctgataactt taatgtttta aaggcatatg atggacttga agcattggac
     5041 atactaatcg aaaatgatat taagttaata ataatggatg taatgatgcc caatttagat
     5101 gggcttaaaa ctacacttaa aataagagaa aaaaggaata taccaataat tctcttatct
     5161 gcaaaatcag aagatatgga caaaattata ggtcttaata tgggtgctga tgattatata
     5221 actaaaccat ttaatccact agaacttact gctcgagtaa aatctcaact tagaaggtat
     5281 ataaatttag gtgattttag taatacaaat aactcaaatg atactaatta tattctaaaa
     5341 agtggtggcc tagagctaaa tacagataca aaaatagttt ctttagatgg tgaggaaatt
     5401 aaaatgacac cacttgaata taaaatactt acacttctta tgtcacgaaa aggtaaaatt
     5461 ttctcttctc atgagattta tgaaaatgtc tggaatgagg atgcttatag ttgtgagaga
     5521 actattgctg ttcatattag aagaattaga gaaaagattg aaattaatcc aaaagaacca
     5581 aaatatttaa aggtggtgtg gggcattggt tacaaaattg aaaaacttaa ttattaatga
     5641 ttctaaagca aaatttattt tattattatt actgtttcta ttagtttcta tattatttga
     5701 agcagtttat gatttgaata ataacttttc ttataatatt aattgttatt ataaagagtt
     5761 tgctctccct tatttttatt ttttcggtac tcctgtacat ttgtcaaaag tagaattaga
     5821 gtttgtctgc ataggtatta tattatcaag ttttttattg actatactat gttttacatt
     5881 gtataaaaaa aataaacaga ctaaagtcaa attcttagag aatgttataa gccttttttc
     5941 taaggtaaaa atagaactta cacttatatt aactttgata ctattaacct taaggtttca
     6001 tgcatttaca agtaataaaa
//
LOCUS       DAELNY010000209         5842 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.212, whole genome shotgun sequence.
ACCESSION   DAELNY010000209 DAELNY010000000
VERSION     DAELNY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.212"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(964..1626)
                     /locus_tag="KPE55_003291"
     CDS             complement(964..1626)
                     /locus_tag="KPE55_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494328.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(1643..4198)
                     /locus_tag="KPE55_003292"
     CDS             complement(1643..4198)
                     /locus_tag="KPE55_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0494329.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNRLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(4431..4625)
                     /locus_tag="KPE55_003293"
     CDS             complement(4431..4625)
                     /locus_tag="KPE55_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0494330.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(4637..4816)
                     /locus_tag="KPE55_003294"
     CDS             complement(4637..4816)
                     /locus_tag="KPE55_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494331.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(4821..5078)
                     /locus_tag="KPE55_003295"
     CDS             complement(4821..5078)
                     /locus_tag="KPE55_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494332.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5338..5842)
                     /locus_tag="KPE55_003296"
                     /pseudo
     CDS             complement(5338..5842)
                     /locus_tag="KPE55_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         1917 a          925 c          593 g         2407 t
ORIGIN      
        1 taactttgta tacttgtaaa tatgaattta aagatgctct tactatagtt catgctaaat
       61 taatttcaaa aaagagacct ataagtaggt ttgtgtaaat gcagacttat ctacagtctt
      121 ttttgaacgt aaaattggta agtatagaat aatattcata ttaaactttt gtaggcaaag
      181 aaatgaaaaa tcaaagagaa ataattatta cagatttaga ttaccaagct gaattaagaa
      241 agtgtaaaac tatggaggat gtagttagtg aagataatat ataatagttt aacttataaa
      301 ttatcttggt gatattaatg aagtttcaaa gtatatatta tttattgata taaataaaat
      361 taaatctgtt gctaatagat gtacctttgt ttggagacaa actattgaag aaatataact
      421 aaaatgttga agtagctacc tgactttatt ttgaaatgcg aacaagattt tggcattaag
      481 ataatttaga aagaatcact ggtacacaag gatgtgaact tagaatgaat agaagtattc
      541 aggcataagg agctttcacc caaataaaac atagtatgaa ctttaaaaga attctaatca
      601 gaggcaatca agatattctt tcagagttta tacctgtagt gttagcctat agtatttcta
      661 agtttcacaa ataagataat cattaataaa tgtagtatgt atattcatcc tctcaaaaat
      721 attttttaat cttacacttt gtaaattatt ctattttaaa atggtatttt tataccttta
      781 tttcttatga ccgttttcaa gctcaaattt tgcataaata taaattaatg aaaagcaagt
      841 aaaaaaagaa ctgtcgcaca gcaatttaaa attactcatt cgacagtcct tatttcgtgg
      901 atttaaatag tggagaagaa tcacaagaat aatacccacg attaaacctt tatacaatac
      961 attttatatg actttaccat cactaatagt tattatacaa tcagccatct tagctatttc
     1021 atcattatga gttatcataa ccaacgtctg cttgtattca tttacacaat actttagtaa
     1081 attcatcacc tcattagttg ttttactgtc taaatttcca gtaggttcat ctgcaaaaat
     1141 tattgatggc ttattggcaa gtgatcttgc tattgcaact ctttgttgtt gaccacctga
     1201 taattcatta ggaaatttat taatctgact ttctaatcct aacttagtta ttaaatcatt
     1261 aatataatta ttatctatct ttcctttagc aattaaaata ggaagaacta tattatcata
     1321 aacattgata acaggtatta gattatagtt ttggaatata aacccaaatt ctttacttct
     1381 aagattagat agcttttcat cacttaaagt atataaatct aaattatcca taaatacatt
     1441 acctaaagtt ggtttatcaa gccctgccat acagtgaagt aatgtgcttt ttccagaacc
     1501 actttgtcca attattgcac taaatttatg gggttctatc tctaaagtaa ttccatcaac
     1561 agcctttatt tcattttctc cttttccata tattttttta atatctataa cttttaacat
     1621 cttcatacat ttacctccca aattattcat tctcttttat accttcaata atattcatac
     1681 tttcaaaatc tctattactt acaactactg ctattaaaca tacaaataca gatatagcaa
     1741 agaatagtaa agattcctta attgggaatg taaaagacat aaaattctct attccatagt
     1801 ttatatacgc atcattattc caatttgtaa atttaactag atttataaat gatggaatca
     1861 tagctattat aaagctaaaa actgcatatg ctaatgcttc atatataagc atttttttca
     1921 tatttttaag actaacacct aatgctctaa gcgttgaaat ttcttttttt ctaattagta
     1981 gatttgttct tatagtacag aaaatattaa ttgatgctat tattaaaact agtgcaatta
     2041 ttgctatttg tgatttaatg taacttttat atatactttc tgacatttca tgataactta
     2101 atctactatt aaatagagac cctggtatac ttcttgaaat tttctgtatc tcattattaa
     2161 caatctcatc atatggattt tctaaattta tgcctaattg agtgtatttt tgttctcctg
     2221 ttatgttttt agagtgaata ttacttgtta taatttctag atctggcata aaatcgccat
     2281 ctcccatagc aatccattca tcctttaaaa ttacagccac tcttaccttc atctctttat
     2341 aaacaattgt attattttct tcaacaggaa tttttacagt tattatgtct cctatattta
     2401 attctttgaa tataggcttt atagtatgat catcaactat atcataaaaa taattatata
     2461 ctgctacatt tggatatcca tcagtgtcct ttttaatatc atctaagcta ccttcttcaa
     2521 caaagtcatt taacttttca tctatataat catcatcata aagctttaaa actaatttat
     2581 cttccattat ctcttttttt tctactccat tataggttgt aaattctttg tttatatcct
     2641 tattattaaa agtaaacatt ccatttgtta tttgaatttt atgtatactt ttaacaccct
     2701 taattttttc tatattctct acatcactat ccgtgtattt tgatatttgc gggtctgcat
     2761 ttactccaaa ttgcattgtt atattatatc tattttgcat atcttcaatt ttactgttga
     2821 cctcttcttg catacttgag aatgtagtaa tgaatatata tcctcctaaa gcaatagaca
     2881 acatagatat agcagctcta accttatttc ttccaatatt tttaagtccc ataaatccat
     2941 aaaatccaaa tattttgctt actagtctgt ctattccaag tctacttctg tgatttttaa
     3001 ttttgtctgt atttattatt gattcaatag gtgatatctt gccgatttta aaagttggta
     3061 taatacaaga tataaaaaca gaaattattc caaccaatat agcttttata actatttcat
     3121 tatttatata taatttaggg ttaagaccag cttcactgta taaaacttcg ttataattac
     3181 taattcctat atatgaatat gtaaccccag ctactaaccc aagtagtata cctattattg
     3241 ttataattaa tgcttgatag attatcatac accttacgtt tcttttcttt gcacccatca
     3301 gtctaaacag tcccatttct tttactgatt gatttagtgt aatattgaaa atattaaata
     3361 tgaaaattat agatactgta gttattaaaa catttctttt aaaaacattc ggatcattct
     3421 tatcatcttc tacataacga agttccctta caagaggtac atttggtaga aaactttttt
     3481 gtcctaactt atattttttc ataagataat cacattcacc ttcaatattt ctccatccac
     3541 ttttaaaact taatatacta ttataactaa tcgcatcatg aggtataatg ttatttgttt
     3601 catcattgcc ataagttact gcttcgtatt gccaaaccac atcatagtac ccttttggtt
     3661 tttccataac tcctactatt ttaaattctt tatttataga aaataatcga ttacttccgt
     3721 tagtatcttt atagttcttt tttattgtta aatttaatgt agatcctact ccttcactta
     3781 aattcattgc ttctagtgct tttttctcta atactatttc attgttgttt ttaggtagtc
     3841 taccttctat tattttgttg tatctacttt ttagatactc ttcgtttgat gaagttaata
     3901 taaatgagtt tcctttttta tctgaaatac gtcctaaatt ttgtaccgaa ttaccctttg
     3961 atactttttc atcagtttct aattttttag catcatcaag tgtcaaatca ctaaactcta
     4021 catggtatcc actgtaaagt tcatgtattg ctactacatt gttttcattt tgtgaatctt
     4081 ttataacact aggtccaaat atcaacataa cacctaacat aactcctatt attaaaagta
     4141 gtgttctaag tttatttttc tttaaatatg ctagtgacat ttttaagtaa atccccatat
     4201 aatcctccta ttttactata aattatttat ccaactcatc tgttaagtag attggtggat
     4261 attttaaatc ttttaattct tcattatttt ttgaagctta ctaccatctt cctcatgata
     4321 aacttgcagt atcaccttat ttacatgtag tgcataataa tcaccactat aaaaatattg
     4381 gtattcataa cctggataat ttttgtaaaa tgatttataa agtacccatc ctaattctct
     4441 tttgtatact tttcttttgt aatacgctct atatcatcac ctgaaactcc ttgatatgtc
     4501 atatatttat ctatatattt ttcagcttca ttccattttc taaaatagtt gttgtatata
     4561 ataaatccta ttatggctat aatgcttata attaaagtta ataaaatagc tttgtatttt
     4621 ctcatagtga atcttactac ttcaatttaa attctttaat aagttttaat tctaaagact
     4681 tagttttatc atcaaatttc ttaacttgaa tttttatatc tcccaaatta tcaacctcta
     4741 tatttccttc acctttatca aatacttctt caatttttct aaaaaattct ttctcactta
     4801 tttctacatg aatcaatgat ctatatgtta taaatagcca ttatgaattt atcatcttct
     4861 gacagttttt cattataaga aaaatcttta acacattgaa ttgataatat ttcctttgtc
     4921 cccttcttgt ccttagtcat tgaataatgt aaaacttggc taggttgttc ttttgtatac
     4981 tccaagtttc ccaaagacaa aacaaaactt ccatctgatt tattttcagc taccctaaga
     5041 ccactatttt ctattgattc tgatattttc tttgacaagt cttcttgata ctagaaatcg
     5101 gttcaatagt atatattttt tcatttgtat ctgaaacctt atttgtgcat cctgttatta
     5161 aacctagcgt taagcttata gctataacca tagttattgt aattttttta tcttcatata
     5221 atccccctta tatattgaaa actcgttatt tctcataaaa aaaagacgct atctttttct
     5281 attaaaagat aacatcccta tatgtttagt atcaatatat gttccccaac tggtaggtta
     5341 atcgacccaa ctatcaaaat tttgatttaa tggtatataa atattcaata taaatttgtc
     5401 ttttttatct tcaaatgata aatctcctct atattttttt atagaagatt tgatgcttta
     5461 aatccctatt ccatgtacaa gtttatcttt tttatctgtt aataataaac ttttattctt
     5521 tctaattgaa tttattttac tattctcaca tttaattacg aaataggact ttgagatagt
     5581 tccttttatt tgtatatatt tatcaatact tttatcatgt atcttatcgc aagcttctat
     5641 tgcattatct aagtatttgc aaatatatta gatacatcta ttggttttat aaaattcact
     5701 ttggaaaaat tgatatcaca aatgaattta atttttcttt tttcacttac atcacttttt
     5761 tcatttatta ttatatccaa tataacatta cctgtattgt aaattgattt aaattcttta
     5821 atttaatctt ttaaactatt ta
//
LOCUS       DAELNY010000210         5801 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.213, whole genome shotgun sequence.
ACCESSION   DAELNY010000210 DAELNY010000000
VERSION     DAELNY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.213"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            288..1907
                     /locus_tag="KPE55_003297"
     CDS             288..1907
                     /locus_tag="KPE55_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0494333.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2281..2475
                     /locus_tag="KPE55_003298"
     CDS             2281..2475
                     /locus_tag="KPE55_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0494334.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(3034..3357)
                     /locus_tag="KPE55_003299"
     CDS             complement(3034..3357)
                     /locus_tag="KPE55_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494335.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3402..3656)
                     /locus_tag="KPE55_003300"
     CDS             complement(3402..3656)
                     /locus_tag="KPE55_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494336.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(3920..4201)
                     /locus_tag="KPE55_003301"
     CDS             complement(3920..4201)
                     /locus_tag="KPE55_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494337.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            4514..5323
                     /locus_tag="KPE55_003302"
     CDS             4514..5323
                     /locus_tag="KPE55_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494338.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            5365..5631
                     /locus_tag="KPE55_003303"
     CDS             5365..5631
                     /locus_tag="KPE55_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0494339.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT         2151 a          728 c          694 g         2228 t
ORIGIN      
        1 gttatttttt tacatatttt ttacaattat agttaattct ttgttaaatt gtttgtacat
       61 aaaatactaa aaatctaagt tcaagtattt aaattgctat atatcataaa ttttctaaaa
      121 ttaacaacaa cttaaaaatt taacattctc ttaacgtata cttaacaaaa cattaatatt
      181 tttattgaca aaatttgtta ttctatatta tggaaacaat aaattttagt taataaagtt
      241 tataattcag ataaaaaaat tattaatttc ttaagggagg aaatatcgtg aatatagtaa
      301 ttagccttat cggtggactt ggactttttc tttacggaat gagtttaatg ggagaaggtt
      361 tacaaaaatc agcaggagac aaattaaaaa aaattattga actcttaaca agtaatgttg
      421 ttatgggtgt actggtaggt acagtcgtta caggtattat acaaagttca agtgctacta
      481 ctgttatggt tgtaggattt gtaaatgcag gtataatgaa tctaagtcaa gctattggtg
      541 taataatggg ggcaaatata ggtactacag ttactgctca attagtttca tttaatcttg
      601 aaggaattgc accaattgct ttaggtatag gtattttatt ctatttattt acatcaaatc
      661 aaaaaacaaa gcatttatct gagatactta taggtttcgg tatattattt acaggaatgg
      721 agtttatgaa agatgcagta gctccacttg ctgaatataa agcattcact gatgcacttt
      781 tatattttag taaaaatcct gtattaggta tattggcagg atttgccata actggtattg
      841 ttcaaagttc aagtgcatct atgggtatgt taatagcact agcttctcaa ggaatacttc
      901 ctctatcatc tgctttacct attctttatg gagataatat aggtacttgt gttacttcac
      961 ttttatcaag tgttggtgca agtagaaatg ctcgacgtgc agctgtaatg catttaagtt
     1021 ttaatgtaat tggtacaata atatttatgc ttgttttaaa taaacctata tcagccatag
     1081 ttactcattt tgacccaact gatacagcta gacaaatagc aaatgcacat actttattta
     1141 atcttacaaa tgttataata ttattaccat tttctaaata catagttaaa ttagcaaata
     1201 gacttatacc tattaaagaa actgaatcag aaatagtaaa taatactaag tacttagatg
     1261 aaaggatgtt tagcactcca tctatagcac ttggtaatac tgtacaagaa gttgtaagaa
     1321 tgggtcataa agctacaaac tctttggaac attcaattgc aggattttta aataagagca
     1381 atgaagatat taataaagct tttgagtcag aaaaagttgt caataagtta cagaaagata
     1441 tattgaacta tttgttaaaa ctttctaaag aacctcttag agatgatgaa cgctttagaa
     1501 cagatttgct ttttaatact gtaaatgaca ttgaaagagt ctctgaccat gcagaaaata
     1561 tagctgaatt ggccatgagt gttaaggaaa tgaacattag tttttctgat agtgcgataa
     1621 gagaaatata cgaaatatac aataagacaa ttactaattt taaggatgcc ttaatagttt
     1681 tagatgttaa agattttgag ttagcaaata aagttttaga agttgaaaat gaagtaaatt
     1741 atttagaaaa aacttttaga aattcacata tgattagact aaataatgga agttgtacaa
     1801 ttgatgctgg tgtactttat cttgacttac ttactaatct agaaagaatt tctgaccact
     1861 caacaaatat agtaaaacaa gtactaaaac ttaaacaaaa aatatagtga ttatttttat
     1921 atataatctt aatcaatgag aagtaagaag ctatagataa tgcttatatc atttatggct
     1981 tcttattttt tatgtaatac tcaaattttt atagtattct cttattatta aacagtttct
     2041 gcctagaata ttccatatat atatgacttc tttcataaaa cttgaactgc ccataaataa
     2101 agtcacaagt atacataact attttttaat caattataac caatatttgt tttatttatc
     2161 ttcattttaa ttcttcattc aataatgtaa taatattatt ttttatttgg atactattat
     2221 atcatctaaa tatgttatta tatatttgga tattttcata ttaataggag ggattctaca
     2281 atgataaatt atatggtaga aattatatat agcgaaaaaa taagcgaagt agaaaacatg
     2341 ttgaaaattg aaaatataca atatgaattt acttctcgtg atggctttat attttttggt
     2401 ttttatgata tgacagacgc atatagattt gagactttat gttcagatga aaaaattcga
     2461 tttaaaagaa tataatagat aaaaaagtac atatttctaa actatttgtt tttcaaattt
     2521 aggttgtgtt ttaatgtagt gttgatatta taaacaattt taataataaa aaagtaggga
     2581 cattatatta atatgctcct actttttact catacttatt aatattattt atcaaaatta
     2641 ccctacaaac tggaatttat cattctttca taattttaaa ctatcatatt taaaatatat
     2701 aaaattctac atgatttttg tataaacata atatcaacca ttatccaatt taaaaatatt
     2761 ttttagtaat ttccatttca ttttctctta acatattaat taaactttta tatgtaaatg
     2821 gtgtatttac atattattta atatccaaca attatataaa gaaatgtgct ctttgcctta
     2881 taaagaatta taaagccaat tcgttttatt atattatttt tctttatacc ttaaaaactt
     2941 taccttcaaa ttcttattta tctatatatt ataaaaatgt tcttaacctc ttttacgaga
     3001 ttttcttgac atattatcag ttagataaaa taattaaata atataatcag caagtatgtt
     3061 ttttactctt ttaagtaaat ttccacctgc accccaatca atttttaaaa ttccaattgc
     3121 actgcctgtt gaagtagctt taataactgt ttttcctgtg gtattttcat aaactactat
     3181 taatcctgca tcactattat ttcttgcata atacttctca aaagttgtaa ttagtataaa
     3241 tttatcatct cctaaattat aagaatcact aaaaactaaa tctgcatcaa gattttcttt
     3301 tatcattttt tgtgcctcta agggtgtaat ttttacagta aatatttctg ttgccatttt
     3361 ttatctcctc ataagaattt attttagaca aaacataatc tctagtattt tctgatatat
     3421 ttatcaaaca atatccctat tcctcctaac agagctaata tagaaccaaa atttctatat
     3481 tgagaaatag attggtctag ataaatatta tattggtcag tatccatgat tccaccattt
     3541 tgttgaataa tatgctctgc actaaattca cctaaaccta tacaacagaa taatactact
     3601 actccaatta caaaagcaaa tatacatatt gttaatttaa aatccttatt ttccatagtt
     3661 atccctctca taagctaaat ttatttgatt atttttacta gattttatat tactcttatt
     3721 atacaataca acatatattt acatctattt ccaaaattgt aacctttata gttttatata
     3781 tttaaatttt ttaaacaaaa aatacacatc tataaattat aaatgtgtat tctgtaccat
     3841 tactaataat atattatact tttttacaaa tttctaatta tttataacta ttcaaaaata
     3901 aaattcatat aaaataattc tattgtacaa ttgaaacttt aataatgtct ccattttttc
     3961 tctccaagtg aactacttct ccagttaaat catagtcaaa tgccttaact aatatttcag
     4021 taactttttt accatctact acatcatctt ttccattaaa taaatccaaa gcattattct
     4081 caatcattct ctttgcaaaa tttacaggta actttatatg tattttatcg cctgtatgtg
     4141 ataagactct tattcttaaa aacttttcta aattgtttct tggtatttgt tttgatgcca
     4201 tgctattaaa tcactctcct ttatttttta tttaataata aattttattc ttaaaaatat
     4261 ctttatatgt atttattatg aaagtaacac tattattttc taatcttgtt ttatattcct
     4321 tcttctttaa tatatttatt taataaatat ctatgattta taataattgt atattttgag
     4381 attttacttt acaattattg tatctaaatt taacaaaata tcttattaga atatttcaat
     4441 taaaacaaaa ctggtttatt atataaaaaa taaattagta tacttatttt ttatatatca
     4501 ggaggatata cttatgaata aaataatcaa taacatttta aatacctgca aacagtatac
     4561 atatattttg ccacataaat tgttaagaca gtatatagca cactatacaa tatctattcc
     4621 agatacaagt attaaagaaa atcttacatt aatacctgat gctagtggat gtatgatatt
     4681 taaatttgac aaaaaaggta tagaaagcgc attctgggga gccacaacaa aaactactat
     4741 tgtaaaaaat gatatagaaa atgtactttt tagagtattc gttgagtttc gcccaggtgg
     4801 agtatattat ttgacaggtc tttctcaaag agaatctaca gatttaaaaa tttcacttga
     4861 agattttaac acattattta gcttagaggt taattccatt tttgaaagaa caagtactat
     4921 aaaggaatta gtagaacaat tagatatgtt attcttatca tacttattga aatcaaatat
     4981 agtagatatg actataccaa ttcttgaaaa tgctagaaaa caaaactcta ttatgtctgt
     5041 taaaaacatt tcacaaatta gttgttatag tgaacgacat ttaaatagaa tttttaataa
     5101 ttctcttggt atgagtgtta aatcatacct tagattactt cgtataaacc ttgtattaca
     5161 agaaatacaa aataataaaa taccatttgc aactttagca caagatattg gttattacga
     5221 ccaatctcat tttataaatg attttaaatc tatttgtggt gttaatccaa caacctatat
     5281 aaaaaatttg tccgattttt acaatgaaaa gtataaattt tgatatacaa tcatataaaa
     5341 tttatattta aggagtgact taaaatgata gaatcaagat gtggaatttt atgttatgaa
     5401 tgtggataca aggaaaaagt caattgtaaa ggttgtacag agattgagaa accattttgg
     5461 ggtgaacaat gtcctgtaaa atcttgttgt gaagataaaa accttacaca ttgtggtcta
     5521 tgtgatacat ttccatgtga aatgcttaat caatttgcat atgacaaaga acaaggtgat
     5581 gatggcaaaa gaatttctca gtgcaaaaaa tgggctgtac tagccttata atatactctc
     5641 ttgttataaa ataagttttt gtaaggtaaa ttttttaaac tgttgtagaa atttctgcaa
     5701 cagtttttat aattaaattt aaaggttatt taattaaatt aatagaatag taatttattg
     5761 ataaaatctg attactaaaa ttggaaatat aaaatctcaa t
//
LOCUS       DAELNY010000211         5757 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.214, whole genome shotgun sequence.
ACCESSION   DAELNY010000211 DAELNY010000000
VERSION     DAELNY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.214"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            19..474
                     /locus_tag="KPE55_003304"
     CDS             19..474
                     /locus_tag="KPE55_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0494340.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            523..1107
                     /locus_tag="KPE55_003305"
     CDS             523..1107
                     /locus_tag="KPE55_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG0494341.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1112..1435)
                     /locus_tag="KPE55_003306"
     CDS             complement(1112..1435)
                     /locus_tag="KPE55_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494342.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1587..2105
                     /locus_tag="KPE55_003307"
     CDS             1587..2105
                     /locus_tag="KPE55_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0494343.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2281..2949)
                     /locus_tag="KPE55_003308"
     CDS             complement(2281..2949)
                     /locus_tag="KPE55_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG0494344.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3064..4479
                     /locus_tag="KPE55_003309"
     CDS             3064..4479
                     /locus_tag="KPE55_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0494345.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4702..5160
                     /locus_tag="KPE55_003310"
     CDS             4702..5160
                     /locus_tag="KPE55_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494346.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5157..5741
                     /gene="clpP"
                     /locus_tag="KPE55_003311"
     CDS             5157..5741
                     /gene="clpP"
                     /locus_tag="KPE55_003311"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0494347.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
BASE COUNT         2195 a          655 c          935 g         1972 t
ORIGIN      
        1 ttaaggaggt gagtagctct ggatgaaata gatttaaaaa taattaattc tctaaaaaag
       61 aatagtaggg cttcaacctc tgaaataagc agacaaatta atttatcagt accagcagtc
      121 tcagagcgta taaggaaaat tgagcagtca aatttaattc aaaagtacac tattcaaatt
      181 aatcgggaaa aaacaaaata taagcttttg gcttttatat ttatcaatac tgatttatct
      241 aaaagtatga aagaatttaa gaaaactata ttggaatatg atactgtatt agaatgtcat
      301 catatttcag gagagtatga ttacttaata aaagtattag cagaagatac aagtaatctt
      361 gaagagttta tatcagtgac tttgaaggaa attgggggga ttcaaaaagc taatacaacc
      421 attgttttat ctacaattaa agaagaattt aatacaataa aagatatttt ttaaataatt
      481 tactagtaaa gtgttaatta tattttagga ggaaaataga atatgaaagt tttagttttt
      541 ttagcaaaag gttttgaaac tatggaattt agtgtatttg tagatgttat gggatgggct
      601 cgtaacgatt atgggcatga tattgatgta gtgacttgtg gatttaaaaa acaagttatg
      661 agcacattta atatacaagt attagttgat aagacgatag aagaagtatg tgttgatgat
      721 tatgatgctc tagctattcc tggaggattt gaagaatttg gattttatga tgaagcatat
      781 gattcatctt ttttaaatct aataagggag tttaattcaa aagaaaaaat aatcgcttct
      841 atttgtgtgg cagcattgcc agttggaaaa agtggtgttt taaaaaatcg taaagctact
      901 acatatcatt tgaaaaatgg taaaagacaa agacagttaa gtgagtttga tgtaaatgta
      961 gttaatgaac caattgttgt agataaaaat attataacct catattgtcc agaaacagct
     1021 ccacatgtag cttttaagct tttagaaatg ttgacatcta aagaacaaat ggatgaagtt
     1081 aaattagcaa tgggatttaa actgtaatgg tttatagatt ttctaagtaa tcagaacccc
     1141 agtcacacat ggaatctaaa attggaataa tactctttcc aaaatcagat agcgagtatt
     1201 ctacttttgg aggaacaaca ggataaacca ctcttataat taaaccatct gattctaatt
     1261 cacgtaattg ttgagttaac atcttaggcg ttgctttagg aattaatttt tttaattcat
     1321 tgaacctcaa tatagtgtct ttaagatgcc aaagtataag tgtcttatat ttaccaccaa
     1381 tcaatgcaag tgttgcttct attgggcaac tgtaattatc taaacatttt ttcatttttg
     1441 ggctccttta tttattttaa agtatctttt taggtactat attacaaaaa agtgcattat
     1501 tgtaatatag taccatagtg ataaaattat agcataggtt aaataaatca tcaacttagc
     1561 ctaataaaac aatagggggt aaataaatga atttcgttga acttgcaaaa aaaagatatt
     1621 cttgccgtaa ttatcaagat agaaaggttg aaaaagaaaa acttgagaag gttttagatg
     1681 ttgcaagaat agctccaact ggtggtaatc gtcaacctca aagattgatt gttattcagg
     1741 aaaaagaagg tataaataaa ctttctaagg ctgccaatat atatgatgcg ccattagcaa
     1801 ttcttgtatg tggagataaa gacaaagttt ggacaagacc ttttgatggg aaacaactta
     1861 cagatattga cacaagtata gttacagacc atatgatgtt acaggcaact gaacttggat
     1921 tggcaagtgt ttgggtgtgc tattttaatc cagatataat tagagaagag tttagtttgc
     1981 cagataatct agaaccaata aatatattat tgatgggata tgaatctaag ataccagaga
     2041 gcccagagag acatgaaaaa actcgtgttc ctttaagtga aattgtttct tatgaaactc
     2101 tttaattata aaattaagtt attcatagga taaatgtaat aaagtgtttg ttataaacaa
     2161 aattcaaaat aaataatatc tacatataat taattttagt gaagtatatc tagtttaact
     2221 agaatgtata cttcactaat ttatttattc aaaatattta tgaggatttt aatatattaa
     2281 ctaaaatata tcaattgaat taacttttaa atctggaaaa gcacttgaaa tatagtgttt
     2341 tatatcattt aaatggtctt gaatttgctt ttctgatatt ttatcaggtc catatacaaa
     2401 atacataata tttttagtat cttttgttat agctgttcga ttatcagggc cattaagaat
     2461 actagataat ataccaatat tatctgataa aaataaatca tcttttgtta aaatatgttc
     2521 tttaccacct ataccataaa aatgttcact tccatttgct aattctattt tcaaaggtaa
     2581 ttccatttta tcaaggtcat gacctgctgt tagaagaaga tttttcaatt ctgctaaaaa
     2641 cattgactca attgttacac ctacatctgg gatacctttt gattttaata gaatagattc
     2701 taattgaagc aatacagggt aagttttctt gaatttttta taatacttta tataacttga
     2761 aacaggctct gttttaattt tttctttgcg attgtattct gggtgttgtt ctataatatt
     2821 tttaattgta gagtttttaa gaactaaaaa attttcttta ttaggttttg aatgtgtaat
     2881 atttataacc ataactccaa atttaatatt gggataaatt tctttaactt tatgtgatac
     2941 ctctaacata atatagactc ctttttattt tatattttat gatataatct tagcaaatta
     3001 aaagtggatt ttatagttcc atttttatag taattttata gaaccacttt tggaggtatt
     3061 agaatgtgga ttacgattaa tagaaatagt aatatatcat tatcaagaca aatttattgt
     3121 gagataaaag atatgattct ggatggaagc ttaaagtttg agcaaaaact gccatcatct
     3181 aggactttat caaaagaact atctgtatct cgtaatacag ttttagatgc atataatcaa
     3241 ttaattgcag aaggatattt ggaaacaagg aatggatatg gaacaattgt agctaaagga
     3301 atatcagggc ataaagttga ttttaagtta aatgatactt ttagagatga aaaagattta
     3361 catactaata aacaaaaaaa ttatatagat tttcgttcag gagttccaga attaaaacta
     3421 tttccacgca aagaactttc aaagctatat aaacaaatac tgtatgattt atcagattcg
     3481 gatttcagat atggaagtac agctggtgta tgggttttaa gagtggaaat atctaagtat
     3541 ttatttcgta ctagaggaat tagatgtaat cccaaaaaca ttatgattgt ttctggttca
     3601 acacaaggtt tatctttaat ttcacagctt ttatacaaaa gtaataaaaa ggttgctgta
     3661 gaagacccta ttcataaagg cttattaaag gttatttcat ctattggata ttcagttgtt
     3721 ggaattaaag tagatgataa gggaatgaat accaacttaa taaaatcaat aaaggggatt
     3781 tcttttgttt atactacacc ttctcatcaa tacccattag gagggatttt acctattcaa
     3841 agaagactag agcttattaa atgtgcaaca gagaatgatt actatataat tgaagatgat
     3901 tatgatagtg aatttagata tgagggtcaa ccaattagtt ctctttatga acttagccca
     3961 aataaagtaa tttacatagg ttcttttagt aaaatattat ctccagctat tcgtgttgga
     4021 tttatgttat taccagacaa tattttagaa agctataaaa ccttaaaaat gtactctgat
     4081 gttcatacag aaacaatatt gcaacatgta ttagctgaat ttattggaaa tggaggttta
     4141 gagaagtaca tatttagaat gaagaagttg tacaatagaa aacgtaagta cctcataaaa
     4201 gttttaaaca cggaatttaa aaatgaattt aaaataaaag gtgaagcagc agggcttcat
     4261 atagttgctc atttctataa tgttaaattt acggaaagtt taattcataa aatttttgaa
     4321 gcaggtataa aagtttatcc tatacaacaa tatgcaattg agaatatatt agaatatgac
     4381 tatgaaatta tactaggtta tgctcatctt agttttgatg aaattgaaaa aggtgtaaaa
     4441 atactcagca aaataataca tatgaatcaa ggtacataga taaaacatgt agcaatctac
     4501 atataataga tgtaattaag tttaaacttt aaaagaaata tatattaata tttttgtata
     4561 aaaatacctg atattttttt atcgatactt tattgctata cactaaatta agatagcaat
     4621 ttatattaag aaaaaacttt ttgcataggt tataatactt aaaaggagag gatagagttg
     4681 aaaacaaata cagatgttat tatggagata attgattata tagaagaaaa tcttacagaa
     4741 aatctaaatt tagataaaat agcttatatt gctaattatt caaaatacca tttacacaga
     4801 atgtttacaa atgttgtagg atgtactatc catcaataca tacagaggag gagacttact
     4861 gaagcagcta gacaacttat atttacagac aaatatatta tagacatagc tattacagta
     4921 ggatattcta cacatcaatc ttttacacta gcatttagaa atttatataa agaatctcca
     4981 caagtatata gaaataaaga tgaattttat cctattcaac agagatttgt tttaaatcta
     5041 aatcatcaaa ttcaaagtga aaaatgtatt tcttatgcaa atatgaatta taaacaaaga
     5101 agagatgaaa tcaattctta tcttaacttt aatattaatt gtgaggtgaa agtagcatga
     5161 gtttagtgcc acatgtaatt gaacaaacag gtcaagggga aaggtcttat gatatatatt
     5221 caaaattatt aaaagataga attatattta taggagaaga aattaatgat gctattgcaa
     5281 gtttagtagt agctcaatta ctgtttttag aatcagaaga cccagattca gatataatta
     5341 tatatataaa tagccctggt ggctctgcta catcaggatt tgctatatat gatactatga
     5401 attatgtcag atgtgatgta tcgactattt gtattggtat ggctgcaagc atgagtgctt
     5461 ttttacttgc aggaggaaca catggaaaaa gatgtgcttt acctaattca gagattatga
     5521 ttcatcagcc aatgggagga gctaaaggtc aagcaacaga tgtaaaaata gctgtagata
     5581 atatattgaa aataaaagaa cgattagata aaattctttc tgaaaataca ggtaaatcaa
     5641 tagaagatat taggagagat acagatagag ataattttat gactgcacta gaggctaaag
     5701 agtacgggct gattgattat ataatgaata ggaatgaata aagtatcaaa gtatcat
//
LOCUS       DAELNY010000212         5623 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.215, whole genome shotgun sequence.
ACCESSION   DAELNY010000212 DAELNY010000000
VERSION     DAELNY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.215"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(196..879)
                     /locus_tag="KPE55_003312"
     CDS             complement(196..879)
                     /locus_tag="KPE55_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG0494348.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1177..1767)
                     /gene="zapA"
                     /locus_tag="KPE55_003313"
     CDS             complement(1177..1767)
                     /gene="zapA"
                     /locus_tag="KPE55_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG0494349.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1792..4185)
                     /locus_tag="KPE55_003314"
     CDS             complement(1792..4185)
                     /locus_tag="KPE55_003314"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG0494350.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMSGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4202..5221)
                     /gene="pheS"
                     /locus_tag="KPE55_003315"
     CDS             complement(4202..5221)
                     /gene="pheS"
                     /locus_tag="KPE55_003315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG0494351.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5271..5505)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1718 a         1093 c          602 g         2210 t
ORIGIN      
        1 tctattaatc tatatattgc ctactatact taagacattt taattaattt atacactgct
       61 tactatactt aaaatacttc tattaactta tacattgctt actatatttt aaattcttta
      121 attaatctat acaatatttg ctatatataa agtatatcaa ataacagctt catttttttg
      181 taaatttaca gaaagttaat tttcattaaa tgaatcatct ttattgataa acataattcc
      241 aaagaccact gccaaaataa aaaaacatat tagtattgat ttttccacga aaatttttgt
      301 aaaaaaagtt cctaaaatag ctccagatat aaagaatata ataatagcat aatactgtaa
      361 actctttttt atcataaaaa tattcttagt atgtatataa tgaaataaag tttctgtagc
      421 acttcttaaa tttccagtac acattgtcgt tgcaaaacta ttcccattta cttttctaaa
      481 actctctact tgcattgaac aaataaaaga aatagcaaca tttacaaaca tattaaatct
      541 tcctctagga ataaatgaaa caataagtat tgtaatcatt tcaattataa tgataacctg
      601 acgccaatga atctttgtct tattatttgt aaaatacttt cttatcttct cggctataaa
      661 tacacctaac ataaatgcca aaataggaat caaataataa aatgattcct ttacattacc
      721 ttctgctata ttaagcccaa acaaaactat atttccagtt tgtgcattag caaagacttt
      781 acctcttacc acataagtat aaatatccaa aaatcctcct gttattgcca acaaagaaca
      841 taaaaagaat gattcagaca tctgactctt gcttgtcata tataaacctc tcgctttcta
      901 atgatagata caaaatcaaa atcattttag attctatttt tcattgatat aaaaaaatta
      961 tttatattta aatattgtct atatttatac atcatttata tttatacatt atttgtattt
     1021 atatagcatt catatttatt tatatttaaa cattatctat atctatatag catttatatt
     1081 tatttacatt taaacattat ctatatttat atagcattta tatttataca ttaaaagaag
     1141 ctgtctcaaa aaacaagaga cagcaaatct tttagtttat tccacatttt ttgcatcatt
     1201 atttaaccct gtacatttta actgtaaatc ataagctttg ttttgaaact cagaagctaa
     1261 gttttcagct atagtagctc tttcatttgc ttcttcaagt tgagtactta actcctcaat
     1321 cttgttttta taagcatcta actctgctct agaaccttct gtcgtattac taagttcgaa
     1381 tatttcttgc tttttatttt ctataatctc aattaactct ttcatcttag tttcagcttc
     1441 acgattttct gcttcttttt ctgctatcgt aagctttaaa gacttgattt ctaattgaag
     1501 ctcttcatta gatgctccaa ccttcttact caattcttca ttagctttaa tcaacttctc
     1561 attctcatct gaacattcaa ataaaaggtc tgatatattt aatgcagtta atatcgcagc
     1621 aactgataag ctcaatttcg ggttttgacg tgttatttta tccatttctt tgtctacaaa
     1681 atcagcaata ctaatcataa acttctctga tttgtctcca accattggat attcagcgcc
     1741 atggatttta accattactt tgttcatcct aacctccccc gacattagtt gttaatttga
     1801 tcttaagtta gcatttaatt tttcgcttaa ttcacttaat atcttatcat gcaccttagc
     1861 tacatcttca tctgttagag ttttatcttt acttctataa gttattgaat atgcaattga
     1921 cttgtatcca gcttctattt gagaaccttt gtaaacatca aataacttgt agctttctac
     1981 taatccttga ccattttcct ttattatatc ttctatttgt tttacaaata catcatcttt
     2041 aacaataaga gctatatctc ttgatgtaga tggatatttt ggtaatggca cataattctt
     2101 tgttctatca gaattatcaa atacaaagtc aatatctatt tcagcgacat aaactctttg
     2161 acctagattg tagttttcta taacatctgg atgcaattca cctaatgtac ctacatattt
     2221 attattatac actattttag cacatctacc agggtggaag gtagtattat tatcttgagg
     2281 ttcaatttca taacctttaa atccgacatt tacaagtata gtttctatag ttccttttag
     2341 tgcaaagaag tcaacatctt taccatacat acctacacac attctatttt cttccttagg
     2401 taattctgaa tcttgtggga tgaatatatg tccacactca aatgcagata cttcttctat
     2461 tttatgagat acattagttg ataaaacatc taacatattt ggtattaaag tagttctcat
     2521 tacagaagtt tcttctccta atgggttaag taatttaaca aaatttctct ttgcattacc
     2581 ttctggtact cttatcttat ctactccctt agggcttaca aatgaatatg ttaatatttc
     2641 atttaatcct acagcagttg aattatcttt taaagcatcc atgaattttt gttttgaagt
     2701 tttaattcct gctgtcgtat ttccttctaa ttgtacagaa ggtatattct caaatccata
     2761 aattctagct atttcttccc atacatcagc ttcttgttcc atatctgttc taaagcttgg
     2821 tacatctatt tctaatttat cattagctac caaattacat ttaaactcta atgattctaa
     2881 aattcctaca aactgctcca ttggtacatc tacacctaat agatggttaa ttctttgagg
     2941 atttactacc aattttttag gttctggttt atttggatat acatctacaa caccttttaa
     3001 aacttttcct gctcctaaca tttcaacaag ctgtgcagct ctatttgctg ctatctctgc
     3061 aagattaggg tctaagtctt tttcatttct tgaagatgct tctgacctaa taccaacttt
     3121 tttagctgtc attcttatgt tttctggttt gaaattggca ctttcaaaaa gtacagtctt
     3181 cgtattagaa gttatttctg agttagcacc acccattaca ccagctaaac caagtgattt
     3241 ttctccattt gttataacta gcatatcttt gtctaatgtt ctctcaacat catctaatgt
     3301 tacaagtttc tctccatctt tagcatttct tactactatt cttccagtct ctacttgatt
     3361 tatatcaaaa gcatgaagtg gttgaccaag ctctaacatt acgaagtttg ttatatcgac
     3421 tatgttactt ataggtctta ctcctgcttc tgtaagtctt ctttgcatcc aatatggaga
     3481 aggttctatt tttacatctg taaccattct agcaacatat cttctacata aatctggatt
     3541 atctatctca actttgaaat ctatctcttc gtcactttct tttacttcga tttcaggata
     3601 ttttactttt gttcctatag ttgcagctgc ttctctagct atacccatca tgcatttaca
     3661 atcaggtctg tttgaagtta attcaaaatc tattaaagca tcttttaatc ctaaaacatc
     3721 ttttatatct tttcctaatt cataagaatc ttcgtggtct aaaatatata taccacctct
     3781 tttatactcc tcaatgtaat gttcatctat acctagttca gctgctgaac acatcatacc
     3841 atctgataat tcacctctga aatcagtctg tttgatttca actcctccag gtaacttaga
     3901 accatttaca gctactggaa tataatctcc ttctgatata tttgtagctc ctgtaactat
     3961 ttgaacaact ttatctccaa tatctacttt ggttacaacc aacttatcag catttggatg
     4021 ttgttttatt tctaaaatct ttccaaccaa tatattttct atttcttcac cataataatc
     4081 tattgtttca actttagttc ctgacattgt cattttatca gcgaactctt ttacatccat
     4141 gtctatatca acatagtctc taagccattt taaagatact aacatctaca taccctccta
     4201 attaaaattg atttaagaat ctcatatcat tttcgaataa taatctaata tcatctattt
     4261 catatttaag cattgcaagt ctttcaaccc caactccaaa tgcaaatcca ctgtaaactt
     4321 ctgggtctat tccacaattt ctaagcacat ttggatgaac catacctgaa cctaatattt
     4381 ctatccaacc ttcatattta cacattgggc aacctttacc accacatttg aaacaagtaa
     4441 catcaacctc tgcacttggt tctgtaaatg ggaagttgtg aggtctaaac ttagttttga
     4501 tatcagaacc aaacaatttt tcaacgaaga tatccatagt tcctttaaat tctgccatag
     4561 taacatcttt tccaacaaca agcccttcta tctgatggaa cattggtgag tgtgtagcat
     4621 ctggcgagtc tgacctaaaa catctacctg gtgcaattac ttttattggt aactcttgac
     4681 ttctcatagt tcttacttga actggagatg tttgagttct aagtaatacc ccatcattga
     4741 tatagaatgt atcgctcata tctcttgatg gatggtcttt aggagcgttt aatgcgtcaa
     4801 agttgttttc aacagtctca acttctggcc cttctgctat agagaatccc attccgataa
     4861 atatatctgt tacttcatct ataatttgag ttattggatg cttcttccca acctttaaaa
     4921 cttttcctgg ttgagtaaca tctatcactt cctcttttaa ttttctttct ttttcaatag
     4981 catttatttc ttctttttta gaatttatgc taagttcaat gttttctctt acctcattgg
     5041 caaccttacc aactactggt ctttcttcag cagataattt acccatttct ttaagtatgg
     5101 cagttatctc accttttttt cctaagtact taactcttaa actttctaca ctttctatgc
     5161 tttgtgcttc ttttatttca gccaaagctg cttcacgtaa agcaagtaat ttttcttgca
     5221 cacatatcac cctttcgttt ttttgtataa aaaaagcccc tcaatccctg tctagggacg
     5281 aaaggctata gtttcgcggt accaccctag tagtttaaca atttaaaaat catcaaacca
     5341 cctctaacaa acttaacgcg tttttgacgt caaagcctac tgtaaaattt cagctctgtt
     5401 actccagagg gaacttctca tgacctctta gaaaacactc ccagcctagg tgtttatctc
     5461 tttactaagt ttaatcaaaa tactttcctc tttcattgca tttacaatat tatattgttt
     5521 attttagtat tatatcattt ccattcaaat tatgtgacta tttttaatat atatccaata
     5581 cataattaat ttaattattt tttatataat atacatctaa tgc
//
LOCUS       DAELNY010000213         5610 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.216, whole genome shotgun sequence.
ACCESSION   DAELNY010000213 DAELNY010000000
VERSION     DAELNY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.216"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(42..2102)
                     /locus_tag="KPE55_003316"
     CDS             complement(42..2102)
                     /locus_tag="KPE55_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0494352.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(2298..2990)
                     /locus_tag="KPE55_003317"
     CDS             complement(2298..2990)
                     /locus_tag="KPE55_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494353.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            complement(3602..3976)
                     /locus_tag="KPE55_003318"
     CDS             complement(3602..3976)
                     /locus_tag="KPE55_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0494354.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            4195..4428
                     /locus_tag="KPE55_003319"
     CDS             4195..4428
                     /locus_tag="KPE55_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494355.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            4412..4825
                     /locus_tag="KPE55_003320"
     CDS             4412..4825
                     /locus_tag="KPE55_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494356.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            4844..5044
                     /locus_tag="KPE55_003321"
     CDS             4844..5044
                     /locus_tag="KPE55_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494357.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILEDIEIDLI
                     IIDEKNNVVWRNEKNTPAHENA"
     gene            5422..>5610
                     /locus_tag="KPE55_003322"
     CDS             5422..>5610
                     /locus_tag="KPE55_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494358.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVT"
BASE COUNT         1915 a          848 c          571 g         2276 t
ORIGIN      
        1 gctttcgaca gtaaaaaaaa tatatatact taaatactat ctcattccat atttgttatt
       61 tcttcttcat atttttgaaa ctctaaattt gctttaaata aatctccatc tatttctata
      121 taaaattttc caccttgtaa agctactaaa tctcttgcaa tagcaagccc taatccactt
      181 ccttcagtat tccttgattc atcagctctt ttaaatcttt ccataatctc acttgggtca
      241 aaatttaatt cataagaaga tatatttttc atagttacct ttacattttc accatagtca
      301 tatacatcaa tatagactct agtttcttct aaggaatatt ttgctatatt agataaaaga
      361 ttttcaaata ctctatacat tcttcttcca tctgcccata tatacacctt atcatttgga
      421 acatttaatt ttaaatataa attattttta gataattttt cttcaagttc acctatactt
      481 tgtcttaata gttgcactaa atcaatcttg ctaatttgta attctatatt gccactactt
      541 actctactcg cttcaaataa atcttctatc aataatttta gtctttttga ctttgactca
      601 agtacattta tataatcatt tatatactct ggttgtattg attcttcttt tttaattaaa
      661 tctacataat ttattataga agtaagtgga gttttcaaat catgagatac atttgttata
      721 aggtcagact tcattctttc actttttact tgctcttcta tagctctatc aagaccttct
      781 ccaatattat ttatattttc agctaaagac gtaaaattat cattcccagt aatttttatc
      841 ttataatcta attctccctt ttttattctt tctgtacctt ctattacgta actaagatat
      901 gaaagtcttt tagttatata tattgtaaat cctaaaaaaa tacatataga aaacagtaat
      961 cccataccta tacttcgagt gctaattacc atgattaaag ctaaagatat tatcactata
     1021 acaataactt tccttgctaa agatactttc tttatagcgt tactgacctc ccttacagtt
     1081 tttattaacc ataaaatcac tcttactata atacttgtct taaatataca cattttattt
     1141 tctaaataac tcaattgcac atttataaaa aatatattat ttgatactat cagtaaagat
     1201 aaagaaacta atgcaattac atcaatatga taagatacag caagagcata tatagttaaa
     1261 attgcaattg aaatagatac taatattaca ttaaattcca taggtatctt actgtatatt
     1321 agtaaaaata cattatttct ttttaaaact tcacctttta ttttgatata taaaattata
     1381 gataataaac atataattgc taaacttaaa ctagctacaa aaaataatct tactttttct
     1441 gtagcttgtt taaatatttg aaaattagta tatactattc catcttctat agttggctct
     1501 tctggaaaag atacatatgc ctcaaaacct tcatctactt catttagttc cagactcaga
     1561 ttattcagtt caaatttttc tccatttatt gttttaatat atgagctctt tcctatctta
     1621 tttattatct ctacattaca atagctctta gttttttgtt taaaatcata taaactagca
     1681 tatgacgtat ttgtataaaa tactcctgtg tatgtattaa taacatacac ttttatattt
     1741 tttagataac ttaaatattc ttcagaagct tgttcattaa tcatgatttt atcatactca
     1801 gaatcactac tacttaaatc tatactttta ccttcttgtt ggaatttacc tttttcttta
     1861 ctagttaatt taccacctac agatagctta aacttgggta atagacttgc cattgttcct
     1921 tcgtcaaata tattactatc ataaaaatca tcagatatat caaaaatccc actctttgta
     1981 gaagtttttt gtatattgta tttaggctca gcaagtccta ttgtcaccaa caatatagtt
     2041 aatggaaatg agatagatat tatgattaat gaaactaact ttaatactct atatttctcc
     2101 aagttgccca cctcatctac attgtcagta ttttatataa aataaagttt aagaaaagca
     2161 taatccccat agagtaaatt aaactctcag agaaaaaatt aaaacatatt aaaattccta
     2221 acatttgtgt aaatgtaaaa atgactaaaa tcaaaatgca attaaatatt aaactctttt
     2281 cttttataat ttttccacta aaatttttca atcttataac caactcccca caccaccttt
     2341 aaaaatcttg gttcttttgg atttatttca atcttttctc ttattcgtct gatatgtact
     2401 gctactgtat tttctgaatt aaaactttcc tcattccata ctttttcgta tatctcatct
     2461 attgagaaaa ctcgtccttt attttctaaa agaagttcaa ttattttata ctcaattgga
     2521 gtcattttta tgttttcacc atcaatttta acttcttttg ttagtgtatt taactccaaa
     2581 gctccacttt ttaaaatttc tttacatgta tcttcataat tacctaaagt tacatatcgt
     2641 cttaagtttg atttaaccct agctataagc tctaatagat taaatggctt tgttatataa
     2701 tcatcagctc caatatttaa tcctattatt ttatctgtat cttcactttt tgctgaaatt
     2761 aaaattattg gaatatttct ttcttctcgt atttttacag ttgctttgat tccatctaat
     2821 ttaggcatca ttatatccat tagtattaga tgaatgtcat tctctgttat tatttccaaa
     2881 gctttcattc catcatatgc tttatatatt ttaaatcctt ctgatttaag atatatttct
     2941 atcgaatcaa caatttcttt atcatcgtct actactaata tattgtacat attttcactt
     3001 cctctattgt ataataatac ttatctatat tatacaatat ctttcactcc ctttctcaac
     3061 aatgactaaa ttgttatatt actttggctt acatttttgt aactttccct tataactata
     3121 taataaaata taaatcttaa gaaaatatta tcctaattct taaatatttc ttaagattta
     3181 agataaattg aggtttattt gaataaattg atttgaatta tgttgttttc agattacttt
     3241 catttaagta ttttgattgc attcagctga attgatgtat attaattctt attgaacagt
     3301 ttttattttt tgggtggaag aatggtggaa gaattacatt tcactaactt tcttccacca
     3361 ttcttccacc caaatatact tagaaacatt catttcactg caataatagc tacttattta
     3421 aattctattt ttatacattc tatctactat atactgtaca tattctatta aaatttatat
     3481 aactaaaata caaacttatt tcattttctt aacatgttca gaataataaa caaaatgaag
     3541 gtagaaaaag ttataatttc ataccttcat tttatttatt atgtatcaat ttgtcatttt
     3601 cttatttcaa attcttaaaa tattcttcga gttcacttaa tttatcttca tccacttcgt
     3661 cgtcttcacc atgtaacgct gacattaaac ttgaaattga atttttatgt atgttcttca
     3721 aaaattcctt tgtttcaaac cttagatatt ttttctcttt tactagtata ttataatgtg
     3781 taagtttgcc tattttctct gtacttaaaa actcccttcg ctctaatctt ttcaaaactg
     3841 taaatgtagt tgattcattc cactcatatt tttcttccat tgcagcaact aattccctag
     3901 aagttaatac cttgttagct tcacaaatat atctcataac ttttaattcc gcttctggta
     3961 atttactaat tttcataatt cgcacccctt tgccgattta tgtatatgtt aaattctata
     4021 tcaataaagt tttacaaaca agagaattaa ggtaaataat tccatccaat ttttaccata
     4081 ttattctttt aggttttata atacttatct aatgagattg acattttact cattttagga
     4141 tattatgaag ttaacttcac atgaagttag cttcatatag aaagaggtta tggaatgaaa
     4201 acaaaagtac gagaatttcg aacaaatatg ggtctaacac agcaacaact cgctgattta
     4261 gtccatgtat caaacagaac aattatttct attgaaaaag agcaatatag cccttctctt
     4321 atgcttgcct atcgtatggc acaagtgttt ggtacaacga ttgaagaact atgttgttta
     4381 aaagaaaata aagaaatgga ggataaaaaa catgaaagta aagagtaaac gaagttttat
     4441 agtgggtatt atagtttgta tgttatgttg tgctagttta gttatttatt gcattttaaa
     4501 agataaaaga ttcctaattt catcatttct tttaattgta atagcaatct ttaatttttg
     4561 taatgctttt tcaagaaaag gcattgttga agaattacac gatagtgcag atgaaagaga
     4621 cttgtactta acaatgaaaa caagtcatat cttagttaaa attatgaatt atacattatt
     4681 tacttttaca tttcttttca tcatagctta tagtgcatgg aaaaatcaat cacttctagt
     4741 tatagctata acactatgtg tcattgagat ttttttattt gttgcatatt tacttatcaa
     4801 tatattttta gagaaaaaag agtaaataca aggggtgata taaatgtatt ttaaaactta
     4861 tatactaaaa agagaagtaa ataacaaaca actagagata actcgaagtt ttgacttaga
     4921 taatctaatt tgtactgcaa cagaaatttt agaagatatt gaaattgatt tgataattat
     4981 tgatgaaaaa aataatgttg tttggagaaa tgaaaaaaac actcccgctc atgagaatgc
     5041 ttagttataa ctatagaagt gtactgcgaa tacacttcta tttttttatt atatcatata
     5101 aattatgcta tcaaaaagag ctatttaaat cgattttaag gtgtgttgaa taagattctt
     5161 gatagtttat atattgatga atttcaaaaa aataagcact ctttattata aggagtgctt
     5221 tggtgtatgt taatcttcta tctaatacaa ttataacatg tcttatgttt tagtatgata
     5281 attttcgttc gctttattat tacaattttt actatagttt tatctatcct gttaatattt
     5341 gtaatatttt atatactctt tgtacttgtg ttactgatat actttgacta agaaaagcaa
     5401 atttaatagg aggtatctgc tatggatttt ggaaatatca atcttatact tattggaata
     5461 atagttataa taggaactac aataatatat ttaattaaac ctaaaactgc tttttgtagt
     5521 aaaaaatatt ttaataaatt agaatcaata tatggaaata tagataagaa aaaaactgtt
     5581 aaactagaag tactttatcg ttatgtaaca
//
LOCUS       DAELNY010000214         5586 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.217, whole genome shotgun sequence.
ACCESSION   DAELNY010000214 DAELNY010000000
VERSION     DAELNY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.217"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            414..893
                     /locus_tag="KPE55_003323"
     CDS             414..893
                     /locus_tag="KPE55_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0494359.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            890..1669
                     /locus_tag="KPE55_003324"
     CDS             890..1669
                     /locus_tag="KPE55_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494360.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDEVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1919..2419)
                     /locus_tag="KPE55_003325"
     CDS             complement(1919..2419)
                     /locus_tag="KPE55_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0494361.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3030..3737)
                     /locus_tag="KPE55_003326"
     CDS             complement(3030..3737)
                     /locus_tag="KPE55_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0494362.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(4540..5409)
                     /locus_tag="KPE55_003327"
     CDS             complement(4540..5409)
                     /locus_tag="KPE55_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0494363.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT         2070 a          821 c          570 g         2125 t
ORIGIN      
        1 ttcattgttt tttcttaaaa gcctactttt agtcaagata ctgttatctt tactcattat
       61 ttaaagaact tattttaaat ctgtacatga agtaaataat ccaaaagcat tgttattcta
      121 actactcaac attaaatact tactattttc taattactca atataaacac ttactataaa
      181 actaaatata taaatggctt ctaaagttta atatatctac atggctttat atatgatggg
      241 aaaatactag aaaaaaatat attatagaat atttgtatag aataggcaaa actttcaaac
      301 ttaaatttat caaataatca taaaaaaagt gaaccatcta tagtaaaatt ttatctaata
      361 agtgaaaggc aaaaaagtag cgctacgatt tgcatagaag ggaaggaact tagttggata
      421 aaactacatt tacaaacaat atacttgaat cagaacagac tttgtatcga gtatctaagt
      481 ctattctagg aaacgaccaa gactgtgaag atgccgtcaa caatgctatt ttaaaagcat
      541 atgaaaagtt ggattctctt aaggaagaac aatactttaa aacatggctg attagaatag
      601 tcataaatga atgtaattca ttaagacgca aacgattaaa atctctatca tttgaagatg
      661 tcttcaaaaa caaaaaaatt gatgaaaaag atgactatag cgatttgtat actgctattc
      721 aaagtctacc taaaaaaatt aaaattccaa tagtccttta ctatattgaa ggttattcag
      781 tagatgaagt taaggaaata ttagatatac ctcaaggaac tgtaaaaagt agattatcaa
      841 gaggaagaag attattaaaa actaaattag aaaacacgga ggtaatcata tgaataaaaa
      901 tatattaaat aattcgtttc cagaagttcc agaaagtttt cataatagtt taagcaaaac
      961 actaaatagt ttaccagaaa gagaggaaaa ttataaaatg attaataata aaacacacaa
     1021 attaccattt agaaaaggct tagttgcaac tttagccatt actttgatat tgagcacaac
     1081 agcccttgca gtaggacata tatatagttt ggttggaaaa tcaagtacta aggctaccta
     1141 tacatctatc ccaactacag aacaagtaaa aaaagacttt aactttaccc ctaatatagt
     1201 ttctgaattt agtaatggat ataaatttaa aggagcatat actgtaaata aaaaagcatt
     1261 tgacaaagaa aataatgttt taggaaaatc aaaagcactt agttttgaat ataaaaatgg
     1321 caatgatgaa atatctcttg acacagcaaa taatgtactt gggaagagtt ctgacaatct
     1381 aaaagttgta gatacttaca aagatattaa tatttcttat gaagcattcg cacaaaagtt
     1441 tgtaccagaa ggttataaaa tgacagaaca ggataaaatt gatgaaaaat caggcaaata
     1501 cacatttagt gtagggccaa ataatgaatc agacaaagtt gaagttaata aatttaagtt
     1561 tttgacatgg aaacaagatg aagtttacta ttcttttaca gttcaagact caaatttatc
     1621 tatgaatgac ttagttaaaa tggctcatga agtaatagat gcaaaataaa cataagtaat
     1681 aataagttgt ttatgagttt attatttatg actttaattt ttttattagt aaacatggtt
     1741 gtatcttcca tctttatatt ttctttaaat ttaatatata aaattttaga tatttagcta
     1801 ccttcaaaag aaccattatt aaaaagccct taaaaatatt tcttttatat ggaattttaa
     1861 gggctttaac tttattttat acaagtgtat atggaatttt aaagacttta actttatttt
     1921 atataagtgt aagctccatt tgaattactt cttcttttac aggtctatca ttccatgtaa
     1981 aaccactaga tattgtattt acctctacaa accctaaaga tttataaaca tgaatagctc
     2041 tactattact tttaaataca tcaagctcaa ttttttcata cccatattct tttgataaat
     2101 caataatttg ttttaaaaga ttacttccga tacctttttc cctatactct ttaagaatac
     2161 ccatgcctaa ataaccaact ttttcagttt taggtaaaac gtcacaccaa ccaatacagt
     2221 tatctgattc taaatcaatt ataaacagtt gtggtaagtt tttatttata atatttttta
     2281 taaattcaac tgtttcttca aaaggaaagg cttcatccat agctagatat ttcctctctt
     2341 ttgctatttt atcaaaagtt tgccaatacg aataaatata cttttcttct gcctttataa
     2401 attttatgcc ttctatcaac ttaacatttc ccctttttaa tttgtgattg tagttaactt
     2461 attttatatg attctagttt ataatgctat ataaattact atatattagc ttaaaatatc
     2521 tttgtatatt tctataaaat taaaaccaat atatctatct cttgtatctc tattaaaata
     2581 atttttaata gtataataaa tatagctctt taatcttttg agtatatata cttaatcatt
     2641 ttgaaatttg atttaaagta taataaaata tataaaaaaa tatataaaat ggtactataa
     2701 aatttttaat cttcatcaat accttttttg ctttcattgt taaaactatt ttttctatta
     2761 gtttatttct tataaaacaa acatttagta ttaaaatata tatcacacaa tagtatttgc
     2821 aatttaactt taaaaaataa aaatacccaa ctgacctaag caactcgatt gggtataaat
     2881 aatctaaaaa atcatttttg tataaactct ttatttaact tacattttta tttaccttct
     2941 actttaattc aacttatatt tctatgcttc ttatactctc attcaactta tatttctatg
     3001 cttcttatac ttttataaat tcatcacaat tatttagttc tcgctatcaa agaaattgta
     3061 ttaaaatact ttccattctc tacttccatc atttttttgt catttatagc atatgaatta
     3121 tcacaattta tccaatcctt ccaagcttca tcatgacaat ttaaagcttc acatttctct
     3181 acaatcacag actccgattc agaccaaagt ttattccacc atgtaattga atggaagttc
     3241 atattatctt gccaaaatgg aatgagttca tctggtatac aatccacgaa atcttctttt
     3301 aatccaggca ttgccatagc aagtattccc ccctctttta caagaggtga tatatgattg
     3361 tctaaaaatc cttctttgtt tccaaaatag tgataagaat cgatacttat caccgcatca
     3421 aagaaacctt ctgcataagg tagttcatgt gcctctgcct gtattggaaa tattttatca
     3481 tccaatttaa actctttaaa tcttttatag ttttcagttg gttctatcca taaatctgtt
     3541 gcaaatacag tagcatcaaa ttcctttgct aaaaatatcg aagatatacc tttaccacaa
     3601 cctaagtcta aaatacgcat tcccttttct agttttatct tacttgttaa ttcttccaat
     3661 atttttatac aattaggtcc catcatattt ttcatgagaa agtccttatc gtatttatta
     3721 ctttttgtaa atatcattct atctttcctt tccctatcag ttattattta ttaaatttta
     3781 tacgtttgat tttttatttt ttatatgctt cttatattga gcaatctctt ttttattcat
     3841 tctttaacag acaattttaa tgtgtaattt tatccactct gtgatagtaa ctttaatgaa
     3901 taatcttatc tactgtgata gttattttag tgagtaattt tatctactcc acagcaatcg
     3961 ctttaatgag taatcttatc cactctatga tagttatttt agtgagtaat tttatctact
     4021 ccacagtaat cgctttaatg agtaatctta tccactctat aatagtcact ttaatgagta
     4081 atcataattt ttatatcatt ttagatactt actctaataa gaagtcattt tctaatactt
     4141 aataagtttt aattacttcc actttaacct atatactaat taacttattt aatttagtgc
     4201 atatttgaca ctttaccttt tattatacca tttattatca taaatataat aattctattt
     4261 ttttaaaata aatatttata ataatctaaa ataaatattt ataaattcta atcaatcgta
     4321 aataataatc taaaatacat caaaaaataa actaaaaaat atcgaaaaat aatttaaaag
     4381 acatcaaaac taaatcttct aatcagcaat taaaagtcta ttttcttagt ttaaaaaata
     4441 aagatagatt ttttaaaaac actggataaa taggtaaaaa aataaacagt tagtaaaata
     4501 aatttttact aactgtttat ttttattgtt taatatgtcc tataaatcct ctaagtaata
     4561 ctttccttgc aaaaattctg attctaaacc tagattttta aaataatcaa ccatgcccat
     4621 acacgcaaat ttttctgtag aataatgagt agcacctata acattaatta aattatcttt
     4681 tgctatttga tggaactcta atactggttc aaaatgtttt aaaggcttag taaacccagt
     4741 aatatataga tttataccaa gttcagcaac ttctcttgct acaaatggat aacttcctcc
     4801 ccctgctgca atagcaacta aaccatcttt taatacagaa tctccataat catacaattt
     4861 cacatcatga ccaacagatt ttttaattaa ttccttaatc tcttctattg attttagtga
     4921 tgttttacat ataactccta cctcaatttt atcgtcatat ttacaaaatg gtttgactat
     4981 ttctaaaccc agagcttttg caaaactaac agaagttgaa tagtcactat agttgtctag
     5041 tggagaatgt aatacataaa aagaaattct acgatttttc atctcttcca tataagatag
     5101 tggtatgtcg tgaaatggaa aaccttcatc tgatgctatg tatcccattg catgatgtga
     5161 aaaaattaaa atatcttttt catctttatc tataacttgc ttaattattt ctttatcagg
     5221 aaaagtagtt gtgtatactt tattaatatt ttgagcatta tctaatacta atcctatgta
     5281 tttttttcta aattctggag taataaaatc attttcaaaa ttcataaaac tccaatcatc
     5341 attaatattt ttaatattaa attctaaatc taatttttta taaagttcaa ttgccttaat
     5401 aataaccact cctttataat aatattgaat tactacatat taatatagaa atttccgata
     5461 ataaacttta tacgtttaag tattcctact tcaaattaaa aatattataa atattcaaat
     5521 ttattaatat attttaatat tatatttatt ttttctttat taatattccc cttattttta
     5581 tttatt
//
LOCUS       DAELNY010000215         5559 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.218, whole genome shotgun sequence.
ACCESSION   DAELNY010000215 DAELNY010000000
VERSION     DAELNY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.218"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(53..1360)
                     /locus_tag="KPE55_003328"
     CDS             complement(53..1360)
                     /locus_tag="KPE55_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG0494364.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(1785..3560)
                     /locus_tag="KPE55_003329"
     CDS             complement(1785..3560)
                     /locus_tag="KPE55_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0494365.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(3557..3694)
                     /locus_tag="KPE55_003330"
     CDS             complement(3557..3694)
                     /locus_tag="KPE55_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494366.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(3772..4341)
                     /locus_tag="KPE55_003331"
     CDS             complement(3772..4341)
                     /locus_tag="KPE55_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0494367.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(4525..5160)
                     /locus_tag="KPE55_003332"
     CDS             complement(4525..5160)
                     /locus_tag="KPE55_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0494368.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         1719 a          995 c          533 g         2312 t
ORIGIN      
        1 ttatttatgc tatttttgtt atacctcata atacaaaaca tattataaag tactagtcaa
       61 ctttattcat atcattcatt acatttaaga ttatctcatc tgttatagac atacctttgt
      121 cacttactct acctaagttt tgtatacttt gctcaacact tgaaccaact ataccattta
      181 gtggtggtac aaaacaatct tgcttagcta ttatagcact ttgtatagct gcagatgctg
      241 cagaagataa ttttaatgca caaccagctt tcgcaccgtc acatatcatt ccacttaagt
      301 cagctatcat attttctata gctccttcta tttgcttttc agtaccattc ataagccaag
      361 agataccagc agttgctcca gtagaagcag caacaccaca tccacataca gcagataatc
      421 ttcctgtata gttcttaata taaccagtta ctagatgaga tatagctaaa gcttttgcta
      481 gtctttcatc agattgtggg aattttttat tataagcaac gattggaaga attgctgtta
      541 atccatgatt tccacttcca tttgaactca taacaggcat ttttacccct gccattcttg
      601 catctgaagc tccagcagtt aacatcattg cataatttat aacatcatta cctaaaagac
      661 cttcttctat agacttttta attccatatc ctacaccaat accagttttt tgttttaatc
      721 catattcagc catctcttca ttcattttta ctccatctag taaaaattct atatctttaa
      781 aatcaattcc ttctacattt tttattaatt cttgtatagt tactgtatcc attaaacttt
      841 cagctttttc tgccgcatca tttgaagctg atacttttgg ttcattgtct aataatactt
      901 ctccatcttt ttctaagaac gtaaaattat catgtttagt tcttattctc actttagcaa
      961 tatgattttt tccttttaac acaacttcta taaatacttt ctctcttgta tcagcaggtg
     1021 ttattgatag tggagtatta tccatataat cctcagctat tttaacttcc tcttttgtta
     1081 gagtttctaa cacacttaat ccattttcag aatgtccacc tactatacct agagctgctg
     1141 caatttttaa tcctaatctt tcagtacctg gtatacctac gcacatacca tttttataaa
     1201 tactaggact aactaacata cgattttcaa ctatttcttc tcctaataat tcctttgcct
     1261 ttgcacatgc tagagctaag gctactggtt cagtacaccc tactgctggt ttaacttctg
     1321 cctttaacat ttccactaat tcttttctta tatctctcat tatatataaa cctcccaatt
     1381 aagtttagat ttagtactca aataattaat taaataattt cttatctata tttatagcaa
     1441 aatacatgcc atatttttta tatcttttta tatttttagt cttatattct ataaccattg
     1501 gtatgactgc ttctatttat gtattttttt tatttacata gagtatttat attctttaaa
     1561 atggagaaac atatgacaat ttcgcaaaaa gacaaaaaaa agtctcaaaa tgagattcta
     1621 tatcattttg agacggctca aatgtattat tttttaactt ataaaaacca ttttttaatt
     1681 cataaaaaaa gattctgcaa aacacttata gtataagttt aaagctttct ttaaagttat
     1741 gaatttagaa aaatttaaat tttatatagc tttttattat attactatga aattatattg
     1801 tatttttcaa tttttctata taatgtagct cttgatattc caagtgcttt agctgttaat
     1861 tctttatctt ttttataatc tttgtactta tttaaagctt ttattatctc aatcttctct
     1921 agttcatcaa gtggaattat cctatcgctt ttaacttcta actcttctac agaggtattc
     1981 ttaatttttt gaggaattac attcattgtg attattgaag aagaagacat attcacacta
     2041 tattcaataa tattctgcaa ctctcttata ttccctggcc acttatattc catcatagca
     2101 tcaacaaccc tgctatctat gtcaataaca tttttatcta atttttttga atactcttta
     2161 accatatgct tgattataag tgggatatct tcttttctct ctcttaaact tggtaatttt
     2221 ataggtataa catttaatct atagtataaa tcttctctaa aagtgccatt ttgaaccatt
     2281 ttttctaaat ctttatttgt agcagcaata attcttacat ctattgaaac atttgattta
     2341 cttccaattt tattaagttc cttttcttgt aatactctca ataactttga ctgtaaatgc
     2401 aaactcatat ctcctatttc atctaaaaat atacttccct tatgagctac ttcaaacatt
     2461 ccaagctttc cacctttttt agctccagta aaagctccct cttcatatcc aaataattca
     2521 ctctctaata aattgtctgg tatagcagca cagtttacag ctataaaagg attatctact
     2581 ctattgctat ggttgtgtat ggctcttgca aatagctcct tacccgttcc actttcccca
     2641 gtaataagta ctgttgatgt tgatttagaa gctattaggg cttccttttt agaatttctt
     2701 atagctacac tttctccaat tatgttttcc aattttattc tataatcttt attcatctta
     2761 ttgtagtttt ttatagcatc tctcttatct ataaaatcaa taacatatcc ctttaactca
     2821 ttttttaata ctattttatt gatgttgtat attcccttcg aatcatatcc gtatctttta
     2881 taaaaaaaac tacaggattt gtccttctta aagttattta tagaaggttt ctttataaaa
     2941 tctaattcga caaatatgtt tttgccagtt atactatctt ttatattgaa aagctcttta
     3001 aattttgaat tatatttatc aatttttccg tccacatcaa tagacacaac agctttgtcc
     3061 atattgttta atagtgtctc taattttttc ttttctaatt caagctcata agttttaatt
     3121 tgtgctttta atttattaga tattaggtct gccattttgc ctaaaaagtt tataagacca
     3181 cttttattat tttctataat ttgtgcttgt tcttttgtaa aggctataag gcctataaca
     3241 ccatgagaat cgccatcaca tataatagga caacacacct ctgcaaactc tacacaagcc
     3301 ccacttttat agcattccct acatatatca ctactctttg ggtcatcaat tagtattgta
     3361 atctgttctg taagtgattt tttaaaagcc gtatatttat ctactttctc acctattttg
     3421 tctatatata ctcctgtacc tgcaatccta aataactcct tatttactat agttacatct
     3481 atattgagta ctgatactat agcttcagct atattttgaa tatcagattt tatatattct
     3541 aaacctatca taatcatcat tccttatttt tctttagcat aagtatttgt cttatttctt
     3601 taagaatcaa gtatatctta tttaaagtaa attggattga catcaggatt acaaagagca
     3661 taacaattat tattaactca taatttattt ccatctaaac atctccttat aacaaaagac
     3721 taggcttaac ctagtctttt atattatatc catatttata ttttaactca tttatcttct
     3781 ttttcccaca taaatatatt ttaatttatt tgactcttta atcataccat caaagtttaa
     3841 tcttgaaaat atattaaata cttcatgtgg atagtgaatc tttgcaacag aaccacaaaa
     3901 tttatgttta agatagttta acttaacttt taaattcttt aaatttaaat cctctaatat
     3961 aacctctcca ttgcccttta acactctttg tatttcagat gaagcttttt cacaatctgt
     4021 aataacatta aattggtcaa ctattattat cttgttgaac tgattatcat caaatggtag
     4081 aaactctaac ttacctttaa ctacattgca attttcatta tacactagag aataacagac
     4141 atcttcataa ttactattct ccagtatagt tacagaagtt ccaagacctc tcaatctctt
     4201 gcctaggttt cctatatgac caacaagcaa tattttatca cctacattat actcaattgt
     4261 atccaaaaaa cattctaatt catcagatgt atatttagct ttaactggct gtttatttat
     4321 ctgacataat atgcttttca caatgcttca cctcttcatt tcctcaatta taaattatgt
     4381 acagattaca ttataataaa tggcgagcaa ctattcaaga gatatttcat tagtattttt
     4441 ttaaacaatc tttatacatt ttaatatgat attataccaa atttattagg attttattca
     4501 taaaaatcag tctatttttt atgtttattc attgttattt tcatccaatt tttttatttt
     4561 cgatatggat acttcatatg ccattttttt gaccacatta ttttcatcta tttttttctc
     4621 atattctcta ctttgaaccc taccccaaag ctgtattcta tcaccaacct ttagattctt
     4681 tgcaaattta gcatttctac cccaaactat tgatggtata taatcagatt tattataagg
     4741 tctgtttata gcaactaata aatcggttat ttcccttcct aaaggtgtct tcctatacac
     4801 aggttcctta catacatatc catctaaaaa tatactatta gggtctttat tttcctcgtc
     4861 tatacttttt atttctctga caaacactgt aagcactaat ctatttttat tatttaatgt
     4921 tttgttatat gatcttaatt ggcctacgac attgactaaa ttttgtttat caaggtcaat
     4981 atcttggaga agtctttctg atacagtcat tggaagtata tcaaaagaat cgcttagtct
     5041 gtttattttt attttcatat tataaaattt ttctccaaat atctcatggc taaattctag
     5101 cttattgtct aactcccctc ttaagtttac tacgttattc tcatctttaa cagctatcat
     5161 attttatccc tccacaattg ttaattctta tatttctggt atcagcttat ataataaata
     5221 tatttataag ggatgtattt tattacaaat ctttgattgc tgtttaaaag tttttattta
     5281 gttataaatt tcatttaata tttcactttt ataattataa aattattatc tttttgtacc
     5341 acatatacta attaaactac ctatttcagt tttgtattaa ttataaaaat cctattatat
     5401 aattaataag tcatatataa cttaaattta ttcactctat ataagcaact tcaacgaatt
     5461 aaatttattg aaataattca gatgaatatt gagtcattat aaacttattt ttcttaatta
     5521 taagttatat tagacttatt acaggttata tttttatta
//
LOCUS       DAELNY010000216         5533 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.219, whole genome shotgun sequence.
ACCESSION   DAELNY010000216 DAELNY010000000
VERSION     DAELNY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.219"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="KPE55_003333"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="KPE55_003333"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="KPE55_003334"
     CDS             complement(385..1569)
                     /locus_tag="KPE55_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0494369.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="KPE55_003335"
     CDS             1942..4293
                     /locus_tag="KPE55_003335"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG0494370.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="KPE55_003336"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="KPE55_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG0494371.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2155 a          628 c          892 g         1858 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aggtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAELNY010000217         5495 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.220, whole genome shotgun sequence.
ACCESSION   DAELNY010000217 DAELNY010000000
VERSION     DAELNY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.220"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            205..711
                     /locus_tag="KPE55_003337"
     CDS             205..711
                     /locus_tag="KPE55_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG0494372.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1124..1792
                     /locus_tag="KPE55_003338"
     CDS             1124..1792
                     /locus_tag="KPE55_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494373.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1951..2457
                     /locus_tag="KPE55_003339"
     CDS             1951..2457
                     /locus_tag="KPE55_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG0494374.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2585..3601
                     /locus_tag="KPE55_003340"
     CDS             2585..3601
                     /locus_tag="KPE55_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0494375.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3955..4659
                     /locus_tag="KPE55_003341"
     CDS             3955..4659
                     /locus_tag="KPE55_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG0494376.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            4954..5433
                     /locus_tag="KPE55_003342"
     CDS             4954..5433
                     /locus_tag="KPE55_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0494377.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         2285 a          471 c          914 g         1825 t
ORIGIN      
        1 tatatacttt ataatcaata tattttatct atattttaca cttataaaat atgaaaacta
       61 cttattgcga aagtaattga tttgttgtat tataatttac atgaattatg taggacaatc
      121 aaaagtcagt attataagag tacatatatt caaattttta acaattaatt tatataatta
      181 aaaaatgaag ggtaggatga agtcatgggt tttagagaag taaaaattga ggaattacag
      241 tttaatccat ttactaagat tggcaaagaa tggttactta taacagctgg tgattcagaa
      301 aagtttaaca caatgactgc tagctggggt ggtgttggag tctattgggg taaaaatgtt
      361 gttacaactt acataagacc acagagatat acaaaagaat ttgtagatag taatgataca
      421 tttacaattg ccttctttga tgaaacgtat agagaagctt taaatatttg tggcactatt
      481 tctggtagag atataaataa aatagaaaaa gcaggactta caccatattt tgtggatgat
      541 acggtagctt ttgaagaagc taatatgatt atagtatgta aaaagctata tcatggtaat
      601 atgccaccag aaaactttga tgctaaagaa aatgataaaa aatggtatcc agagaaagac
      661 tatcatacta tgtacatttc agaaattata aaagttcttg taaaagaata attataataa
      721 aaaatatatg aaaagaactt attataagta aatgactgtg ctttcttatc tgatatctaa
      781 gtagatgcta ctaactaaat aaatattaat tttaaataaa tactattata aaaaagatga
      841 actatattta ctatagttca tctttttatt tatataagca cttatataag tagagattta
      901 tttgaataaa agtatttatt tgaaatattt gagtatactt tataagtatt tttatgctaa
      961 ctagaaacac ttaatttatg gcatttgtac ttgatttatt ttaatgtaaa taatttcaaa
     1021 gttaatttta ttcaatatag tactataaaa gtataaataa catttaataa aaatatatat
     1081 tatattaggt attttaaata aattttttaa agggtgattt tttatggaaa atataactag
     1141 ttattttaca acaatactat gtgtatttat atgcttatca tctgtattta tatttactca
     1201 actagctaga gtatttatta ataagaagaa gattaatcaa aaaattaaat caagaaacgg
     1261 atttaggtat gatagagatt ttattgaagc cagaagagaa gaaatacata taaaggataa
     1321 caataataat aaaaataaga gtaacaataa gaaattaaag gaagaaaaag tatttaaata
     1381 cgataatggt gatttatata aaggtgagtt tgttgatgga aagaaaaatg gatttggaat
     1441 atatattttt tcaggtaagg aaaagtatga aggattatgg aaagatgaca agatgcatgg
     1501 aataggaaaa tatacttata gagatggttc aatatataca ggtgaattta aatacggatt
     1561 aaaaaatggt ttgggaaaat taacatatcc caataatgac atttataagg gatatttttt
     1621 agataataag aaaaatggta agggtgtatt atataaaaat gatggaaata aacaagcagg
     1681 aatatgggaa gatgatgaac aatgtaaaag tttagatttt aaagatttaa ataataataa
     1741 aataaataat gtttatcaaa atagattaaa taacaataaa ttaaaagttt aattattcta
     1801 ataaaaaaat ctttatttat gatggaatat aggatatatc tatgttgtaa tttgtaaaat
     1861 cttcctatat catataaaat taagtattga ttagtctatt acataaatat agaatataaa
     1921 tatatccatt taataataag gagaagagat atgaaagagt atgtagttgt ggatttagaa
     1981 actacaggat tagatccata taaaggttgt gaaataatag aaataggaat aacagaaata
     2041 aaaaatgaac aaattgtaaa aaactattca aggttgataa aacctaaggg gataatttct
     2101 tcatttataa cagaattgac acatataagt aatgaaatgg tagaaaatga agaaccacta
     2161 gaattagttt tacctagatt tagaaaatac ataggtgata gaactataat tgcacacaat
     2221 gcgaaatttg atttaaaatt tttaaattac tatcttagaa tgttagattt agaaccaata
     2281 aacaattata tatgtacagt agagttatta aaaaaatgta aaagttataa aggaaaaaac
     2341 aagaaacttg aaacagcttg tgcttattac aacatagaga atataaatgc tcatagagct
     2401 gacagtgata cattagcaac tgcaaaacta tttttgaaaa taaaagatga atattaatta
     2461 taaatataga gaaaattagt gaaaatatcc tttataaaat tgacccttaa gcttacaatt
     2521 ttgttctttg atttattttg tagaacaatg tataatgtac cttatatgaa gaagggagac
     2581 taaaatgggt tattacaatg ataatgataa taataaaata agacgtaaaa aaattagtaa
     2641 caacaaagat agtaataaaa aatctaaaca ttcttatact tcggcaaata aaaaaaataa
     2701 aagtcctaat acaagttcca gtaaaagaat gagtgaaaaa gtagattcac agaatcttat
     2761 aaaaagagat agagatagtt ttacaagaga taatgtaaga cctttaaata cagattacaa
     2821 tcaaagagaa agaagaagag tcgagtataa acaaagagag aaaaaattaa aaagtaaaaa
     2881 aagaaaaaaa caaaaaagga ttagaatagt tagggcatta ttatctatta tgttagttgc
     2941 actaattata ttttcagtgg caggtgcaat atttacagtt tcagctataa aagggtcacc
     3001 agaagttact aaaaagttaa ttaggtctaa atatataagt agtgaggttg taagtataaa
     3061 acaaatgcca aatgatttaa aacatgcaat agtttccata gaagatgaga gattttataa
     3121 gcataaagga gtagatgtta tttctctagt taggtcagta ttaaacaatg tgtttacaga
     3181 tacaacacaa ggtgcaagta ctatagatat gcaagtttca aaaaacctgc ttactagtaa
     3241 tgataaaact atgaagcgga aaataaggga tatgtataat gctaagcaga tgaataagat
     3301 tatgactaag gatgaaattc ttgaagcata tttaaataat atgtatctag gtaaaaatgc
     3361 atatggagct gcaaagggag ctgaagtcta ctttggtaaa aaagttagtg aattaaattt
     3421 agctgagtgt gctatgttag ctggtatgac aaataatcct gcaaggtata ttgaccatgg
     3481 agaggctaag aagagacaag tgattgtact gtataagatg catcagcttg gttatataac
     3541 tgataaggag tatagaatag caaaagctga tgacactcca tttaaatcag aaattgatta
     3601 aaattgtaag aatatagtat tttcaatagt ttaaacaaac aaaaatgtaa taaaaaaggt
     3661 gatttgccac ccatttgcca ccataattat aatctaatgg caaattgttt taatgtacaa
     3721 actagataat agaatattgt cttaaatgct atattatgct aaaatttaat tgtattttgt
     3781 aaattaaaaa acttataatt tagttttcta gggttccgca ttttataatg gtcaggtcca
     3841 agagaaaacg cgcaattgtt gtgtacacgg aaggataaaa gcctgggaga tatttaagaa
     3901 tatttctata ggctttttta tttttacata ttttaaattt gaggaggttt tctaatgaaa
     3961 aaatttgtta ttgaggatgc attttggaat ctatttccta gtgcaaaaat tggagttgtt
     4021 atttgtaatg atatagataa ctctataaaa gatgaagact ataagaaaat aattttgaag
     4081 ggagaagaag aagcattaaa atacttggag gactcagaat ttagcaacaa ccaagtgatt
     4141 aaagtatgga gagaagcatt taaaaaattt aaaacgaaaa aaggagcaag gtcttctatt
     4201 gaagcattat taaaaagagt tcataatgga aataatatag gaactattaa ccctttagtg
     4261 gatatctaca attttatctc attaaaatat gcattacctt gtggagggga ggatatagat
     4321 aaatttatag gagatattag gctaactaga gcaattggta atgaggagtt tatcccacta
     4381 gggacagatg aaaattcatt tccatatgaa ggagaaataa tttataaaga tgatggtgga
     4441 gcaatttgta gatgttggaa ttggcgtgaa gcagtgagaa caatgcttac tgaagatact
     4501 aacaatgcct ttttatgcat tgaattagta gatgaaagta gatttaaaga atttgaaaat
     4561 gctttaaatg aattagctaa aatagttcaa gaaaaattgg gtgggaattg taaaatttca
     4621 attttagata ttgataataa agaggtatca gtggattaat atggaggtgc tgttgtaatg
     4681 gactattcta gttataaaga tacttatatt tagggtaaga aggtctattt tagatataac
     4741 gattatcaaa ggatataata tagaaaataa aaatgtagaa tataaaaagc ctttaaaata
     4801 agaaaaaaat gtattttaaa ggcttttatt atactctttt ttatttgcat aattaacaat
     4861 aaaaatatat tgttaattat aatattttta tttaaaaacg atataatata atgtattata
     4921 ctatatataa aatattttaa aggagttggc atgatgtcta ataaacttat aaaaaattta
     4981 ctaagtggta tattacaaat tttatttttt cttttaggat tagtaattgt tgtaggagga
     5041 tttaaaagtt ttatgtacct ctgttttagt ggagaagcaa ctctacaagg tactatttat
     5101 ggaatattaa tgtttatttt aggtgtatca tattttatca ttataaaatc attgatagaa
     5161 gtattaggta gttctgaata tagcctattt gtaaaagaga atgtaaaaag atttaggatt
     5221 attggatatc ttttgctttt aaactctcta attgagttta tttcaacttt tggtactact
     5281 ggaaaaggaa tgagatttct agatttagga tttggatttt attttactgt tccagtattt
     5341 gtatatttta taacttctct gatgagtttt gtaattgcag atggatttgt aaaggcaata
     5401 aagataaaag aggataatga tttaactatt taggtgtgac actttaatat ataggagata
     5461 aataacccga aaagagtgac taactagtaa tttaa
//
LOCUS       DAELNY010000218         5488 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.221, whole genome shotgun sequence.
ACCESSION   DAELNY010000218 DAELNY010000000
VERSION     DAELNY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.221"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(198..806)
                     /locus_tag="KPE55_003343"
     CDS             complement(198..806)
                     /locus_tag="KPE55_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG0494378.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(1066..1611)
                     /locus_tag="KPE55_003344"
     CDS             complement(1066..1611)
                     /locus_tag="KPE55_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0494379.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(1729..3552)
                     /locus_tag="KPE55_003345"
     CDS             complement(1729..3552)
                     /locus_tag="KPE55_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494380.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLLIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(3565..5340)
                     /locus_tag="KPE55_003346"
     CDS             complement(3565..5340)
                     /locus_tag="KPE55_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494381.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQNFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRTRIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1847 a          949 c          472 g         2220 t
ORIGIN      
        1 atttattatt atctatttaa atacactatc taatattagt ttcttataag tcataaaagc
       61 acaactaaca ctataattct agtgattaat tttatttcaa aaaatataat aacactacta
      121 atattttaaa tatcaatagt gtcataaaaa cttttcagat taattaaatc tttctatata
      181 ttttataata gtctaaatca tgctaaatat ttagacagaa aacttttttc tttaatagta
      241 aatacttttg tttgaatatc agaataatta tttgaatatc tatctataaa accatgattt
      301 gcatctaaaa tctcaatttg gatattttgt ttcctagaaa gttggcaaga aatagaatca
      361 acatcaaatg aatcgtgctt tgcaaatact aataaggtag gacattttgg aattacatcc
      421 atgtaatctc gaatccgaga gccataacaa caaattatgc catcacaaaa agaatcttca
      481 ctgcatctcc atgcaatagt tgctcccacg ctatacccag caacaaatac atatttatac
      541 tctgatttta aatcatttat aagcttttca acttttttat aaatatcaaa accaacatta
      601 tttataaaaa aatcatatgc ctcttggact tttgaatatg gaaaaactct tttttctccc
      661 agaagttcag gacaaaatac atcatatcca tatttataat acttttgaca aacatcttct
      721 ataaagctat taatcccata gatttcatgc aaaacaacaa tagctacatc acttttatgt
      781 atatacttta tcagattatc caccaccttt acaaaacagc aaatttttta tacttagttt
      841 aatatattct tctttacatg tcaatttact tttctaaaat caaatttatt tttggattaa
      901 tcatttattt ttaaaagttg tgcattatta ctattaaatt attctctatt attaaaaata
      961 taatcgttag aaataaaaaa taaagtacaa ataattaaaa aatatctcac tcataacttt
     1021 aaaaattata agtgagatac tcctaaaatc tactttatat taaatttatt ttccaaaata
     1081 tctatttaat aatcctaaga atgctttacc atgtctagct tcatctttac acatttcatg
     1141 tactgtatca tgtatagcat ctaatcctaa ttcttttgct ttcttagcta attttaattt
     1201 tccatcagtt gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc
     1261 aactacaact tctccaagaa gttctgcaaa tttagcagca tgttcagctt cttcaagagc
     1321 tattctctta taagcttctc ctacttctgg ataaccttct ctatcagctt gtctactcat
     1381 tgctaaatac attcctactt ctgtacactc accaacaaag ttagctctta atccttcgat
     1441 tatttcttca tctacacctt gagctactcc tattctgtgc tcatcagccc aatttttttc
     1501 gcctttcatt tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc
     1561 agcatctcct tcatgtatat atccacatac tgtacaaaca aatttcttca taattacttt
     1621 cctcctaaat tttatatatc aaattaaatt ttttatttat taagttagtc tatatatacc
     1681 cgcaccatat acctttaaac aattttttta aaagtttttt ctaaatactt attttccctg
     1741 tgtcaataat atgtttctat aataatgtcc ctttaatttt atcaattctt tatgatttcc
     1801 tgattccaca attctaccat tttctaaaac tattatatta tcacaatttg atatggtact
     1861 taatctatga gctataatta tacttgtctt tccataagtt aaattattta ttgcttcttg
     1921 aattttagct tctgttgcta tatctatgtt acttgtagcc tcatccaaaa taagtattgg
     1981 agcatttttt aatactgctc tagcaatact tattaattgt ttttgacctt cacttaaatc
     2041 ttcaccccta tccgataata cagtactata tccttcacgc attttaacta taaatttatg
     2101 tgcatttgct attttactag catatataac ttcttcatca gtagcctcca gatttccata
     2161 ccttatattt tccataactg tatcattgaa tatataacct tcttgtggaa ctactccaaa
     2221 acaagttctc aaatagtttc tgttaataga gtatatatcc ttaccatcta aaaatatact
     2281 tccttcatct atatgataaa aatttgtaat tagatttata attgttgttt ttccacttcc
     2341 cgtctctcct atgatggcat ttgatgtgcc agatttgatt tttaggttta tattatctaa
     2401 aattttcttt tttgaatcat aagaaaaact tacatcttta agctcaattt ctccctttac
     2461 aaaatgagag tttaaattac catctttatt atcgttaatc tcacttttaa acatgttatg
     2521 cacttcttgc tcttcttgtt catctataat ttcaaaaact ctttcacatc cagctatggc
     2581 tgaaagtaat gtattaaata gatttgctaa ttcatttaaa ggtcttgtaa attgtcttac
     2641 ataacttaaa aaactagcta taacccctat ggttataata tctttagatg caagaatgcc
     2701 tcctataata catatagttg caaatcctat attactaatc acattcataa taggcattaa
     2761 aagagaagtg taaatttgcg atttaaggga aatatttaga agtctatcat tgacttcttt
     2821 aaaatcattt acagattttc tttcataatt aaaagatttt actacattta tgtttgatat
     2881 catttcttca atatgtgcat ttagttttcc caactcaact tgttgttgct taaaaaaaac
     2941 tctagttctc tttgcaataa aattactcac aaacattact atcggtatta atattacact
     3001 gcaaaaggtt aaaattgggc ttaatacaaa catcataata aaagtcatca atatgtttaa
     3061 gattcctgta attaactgta ctaatgaatt tgatattcct gtactgatat tatcaacatc
     3121 atttgtcact ctactcataa tatctccagt agaactttta tcaaaaaagg ttattggtag
     3181 atttttaaat tttaaaaata aattacttct catttcttta actatacttt gagataatct
     3241 agctgtacat atccccttag acagtttaac tgaaaactca attatataaa atgaaagtaa
     3301 aataattatt ccttttaaga aaatggattt tgaataagta tcgatatcta ttatgtcaac
     3361 tacctttcca atagtatatg gtattacagt tgatataatt gaatcaatta ttaatagcaa
     3421 aaaaataaat ctagtaatct tcttatcttt tttaaagtac ataaaaattc ttttaaacgt
     3481 ttttttaaaa tctttaggct tttctttttt ctttaatcta ctgaccttct gatttccagt
     3541 acttaaattc aatatacttc ctccttaatt tacatcttca cttactaaat tttgcaacgc
     3601 aaaaattttt ttataaattg gagaactaac cagtaaatct tcatgtgtag caaatccaac
     3661 cacctcaccc tcatccaaga ctaaaatctt atcacagtca attatagaac taatcttttg
     3721 tgaaatagtt attacagtac atttcttact ttccttttct cttaaattac ttatatactc
     3781 ttttatattc tttcttataa ttgtctctgt attaatatcc attgcactaa aacaatcatc
     3841 aagaattaat atttgtggtt ttttggctat agctctagct atagtaattc tttgtctctg
     3901 tcctcctgat aaatttttac ccctttggaa taaaacttca tcataagtat tttcgagttt
     3961 tgttataaat tgatttgcat ttgctatttc acatgatttt acaacatctt ctttgcatat
     4021 atctctattt cccataacta cattctccaa tatacttttt ccaaacacac tactattttg
     4081 aaatactaca cctattcttg tccttaaact ttcttcagaa atactgtata tactacgtcc
     4141 tccaatctta atatcacctt tatcaattat atataatttt tgaattaaat ttactaaaga
     4201 agtctttcct gaaccagttt gaccaatcac accaatattt tgaccttctt ctattttaaa
     4261 ggatacgttt tttaatattt tttcatctgt atttttgtta tacgaaaaat ctacattttc
     4321 aaaaattata tcacaattta caaactttaa atctttttct ccaacatcta aactttcttc
     4381 tctctcaatt acttcttcat tctcatcaac caaaacctca cttattctct cataagatgc
     4441 ttttgcccta acaaaaacat taaaaacatg tgaaatcatt ataagagatg ttaatattct
     4501 agtcatatag tttacataag caactataat cccaatttta atttctcctg aataaattaa
     4561 atgccttcca attagtaata ttattattat ggaaacatta atgattattg taattgtcgg
     4621 tctaaaaaat gacatcatag ttgtagcttt tctactattt ttaaaaagtt catcatttaa
     4681 ctcttcaaag ctttggttct cataatcgta tctacaaaat agtttaacta cttttattcc
     4741 acttaaaaac tgtcttaatt ttgaatttac tttatctgta ctttcttgta catttttaaa
     4801 taatctatac cctatcttca tattaagcaa tattataata aatataatta aaactgcaat
     4861 tagaataatt attgacatct ttaaatttaa ttttatagct ataattaaac ttcctataca
     4921 cattattggt gactttataa aaactctcat cattccatta acgaattgtt gcatttgatt
     4981 gatatcatta gtaattcttg ttattaatga agctctacca aatttatcta tttgttttaa
     5041 tgagtatgta tttatctttt caaatacatc ctgtcttaaa tcataagaaa aattctgaga
     5101 aactcttgcc gagattacac ttcttattat tgcaaatatt gcacctaaaa aagttatacc
     5161 tatcattata gctcctgttg atagtatgta gtctaagttc ttatttgaaa cccctatatc
     5221 tattattttt gccattatag ttggttgagc taaatcacaa aaagcttcca atgttaaaaa
     5281 aaatactgct gttagaaata atactatata tttttttatg tattttttaa aatacctcat
     5341 tcaatcatcc cttcatagaa ttttctcatt accaaaagta tatcataatg gctaatttaa
     5401 ttgattatat caattataaa tatttgattt attattaatt tttataccat atattttata
     5461 aattatttgt ttttatttcc tatattta
//
LOCUS       DAELNY010000219         5464 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.222, whole genome shotgun sequence.
ACCESSION   DAELNY010000219 DAELNY010000000
VERSION     DAELNY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.222"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            67..249
                     /locus_tag="KPE55_003347"
     CDS             67..249
                     /locus_tag="KPE55_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494382.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1081..1995
                     /locus_tag="KPE55_003348"
     CDS             1081..1995
                     /locus_tag="KPE55_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG0494383.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2385..3032
                     /locus_tag="KPE55_003349"
     CDS             2385..3032
                     /locus_tag="KPE55_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0494384.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3199..3768)
                     /locus_tag="KPE55_003350"
     CDS             complement(3199..3768)
                     /locus_tag="KPE55_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0494385.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3773..4672)
                     /locus_tag="KPE55_003351"
     CDS             complement(3773..4672)
                     /locus_tag="KPE55_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0494386.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4683..4832)
                     /locus_tag="KPE55_003352"
     CDS             complement(4683..4832)
                     /locus_tag="KPE55_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494387.1"
                     /translation="MSNFIKDNKKSISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4825..5409)
                     /locus_tag="KPE55_003353"
     CDS             complement(4825..5409)
                     /locus_tag="KPE55_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG0494388.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1989 a          830 c          641 g         2004 t
ORIGIN      
        1 tatgagtgaa ataatatctg ctatttactg aatattttta tttttcgttt tttaggggga
       61 ttagttgtgt ttaaagataa gattgatgaa tgtgttcata taatgactgc atatattgct
      121 agtttaaaag aatactattc attcatagaa actcaaattg gtgattttat taaaaaatat
      181 ggagaagatg ttgtagagtt atgtctccat agagtgatga ttctactatg cgaatgtggt
      241 ttggcataaa attaaattta ttaaaatggg taagaagaat cctatataat attcttctta
      301 cccattttaa tatatcaatt tcatagctta tcgtttaaaa tattttacat ccattactag
      361 acaactttac attctctttt taattctagt atatttaaat cattttagtt gctccttatt
      421 tattcttatt acttgtttta ttttataaat aaaataattc tttttaataa tgttttttgt
      481 tgatattcct atcacacatc caattattta tcattgtctt gaaattgttt gcatacttaa
      541 tttaatccaa ttgaaacact ccagcataga atcccctgta aaatagaagt tggattaaat
      601 cctaacatct atacagaaaa tactactcca agcacaagca atataatgga atatatttat
      661 cacttaattt tttatacttt ttaaatccca tacctaaaac attaagtgca gctattagaa
      721 ttaataattg ttgtggtata aaattatgct tgcttcatat ttattcattt tttcctataa
      781 aagttcttgt tttacaaaaa acatttgttt tgtatcaaaa caatacttat aaattacata
      841 attctcataa aaatttctaa tattacttgt actcttaata aataatagcc aacaagcacc
      901 ttaaatcatt atatctatat ctaataagaa acacagtaag ataatttttt cgcccataac
      961 ttaataaaaa ctgttgatat tagtagcaat tgaatttagt taatttattt ataatataca
     1021 taaatttaaa tattacgaat aaagtattat aaataggata ctttctaaag ggaggaaatt
     1081 atgatagata atcaaaaata tgttatttta tcactagaat tacatttatt tttttcaaga
     1141 attatgaaag agcatgctct ttttttagaa gcaggattca caaataaaaa ttataatctt
     1201 gctatggaag ctgaccatta taaaaagcaa tttgaagatt tattatcata cactgttagt
     1261 gctagtaatg gtataattag acctgatata ttatattcag aagagcttgt aactactctc
     1321 acatcagttg cagaacaaaa aacagaagag tttacaggga tagaaataaa caaaaacatc
     1381 actacaagag aattaaatct acaaagtggt gtaaacccac aagttggtca agatttagtg
     1441 aactatgtag ctcaacttaa ctctgatgca ataagattac ttgatgggct tattaatttt
     1501 aaagaaagag tcttagatgg tgtactatca tgtactatat ttacatcaaa ctaccctcta
     1561 cttcttgaac atataataca tgaagcaaat ttatatcgtt cttatgtagt tgaccttgaa
     1621 aataaaatag atattgagtc aaaaaacgct aaagaaatag aattattctg ggaccatatt
     1681 atgatggaac atgctctgtt tatgagagga ttactagacc cctcagaagg tgaactaata
     1741 aatacttcaa atgattttgc tataaaattt aatgaattaa ttgaaaaaac aaacgaaatg
     1801 actgattcta atatcaagaa cattacagaa gaaactctaa atgaaactgt tgagtttaaa
     1861 gattttaaag aagcaggagc atcaggaata gaacagtgta agataaaatc tataatatta
     1921 ccacttttag cagaccatgt tttaagagag gcaaatcatt atattagaat attggagagt
     1981 tataaaaaca tgtaattaaa aattactttt aaaataatct ttgcatattt agataaaata
     2041 aaggtacagt taactgtacc tttatttttt ataaacttat aaaatttaaa caagttattc
     2101 ataaaataaa ataataatca tctataaccc acaactttat tttatattaa ataaaaagta
     2161 ttagtgatta tgttttatag gtatttcttt attcagttat ttattaaaaa ctaatagtaa
     2221 atcttatact aatcgcatat ttatgttata actatattgt aactattttc gtaatcttag
     2281 gaaaaggtta cttataaaat taaccttata caataatata tcgtataatc ttaattagca
     2341 taatagttac aatattaaaa tttaaatatg ggagtgattt tagtatgata agacaaacac
     2401 cattagagaa acgatatgac aagctaggtc ctcatattgt aacagcactc aaaaaacgtt
     2461 attttgatgc ttattactgt aaaactagaa atgaagcttt gcaacaaatt ttagatttaa
     2521 ttcctcaaaa tcatgtagta tcatggggag gatctgaaac acttaaagaa atgggcgtac
     2581 aaactgctgt tagagagaaa ggatttaaag ttattgatag agatttagca aaaactccag
     2641 aagaaagaac tgaaattatg cgtcaagcac ttctatgcga tacttttttg atgagtagca
     2701 atgcaattag tgaagatgga caactcttta atattgatgg aaatggcaat cgtgtagctg
     2761 caatgatatt tggtccaaaa aatgttatag ttgtagctgg tatgaataaa attgtaaaaa
     2821 acattgatga tgcaatacaa agaactagaa caattgcatc tccagcagtt gttcagcgtt
     2881 ttcaagatgt caatacacca tgctatatta atggaagttg tatagactgc acaagtcctg
     2941 aaagcatttg tgcatacatg gttactacaa gaatcagtcg tccagcaaaa aaaataaagg
     3001 taatattggt aggagaaaat cttggtctat aaaatataca tacatgtaaa tagctctata
     3061 aataataaaa tcatatgaaa taacttattt ctaaaagtgg tagaataaaa gcttctacaa
     3121 gactttcatt ctaccacttt tatattcttt taatttgtgt ttctatatta ttaattgctc
     3181 cgattttcat atatattact aactattttt attttcataa tttttttgtt cttcatcaag
     3241 ctgtttataa taatctttat tatagtcagc tagataatct ataccacctc ctgtaatcac
     3301 atttaataat ttcattgcag attcttgatt aactttacaa acataattat caccagattt
     3361 aacaagctct aatgtaatat taaattcttt tttctttgca gaatcaatat ctttcatagt
     3421 tggatttggc atcatttctc ctagtttagg aggattcttt tttaaaatag aaaccatatc
     3481 agtattgtta atttttaaat ctacagtcac tttatcctta ttattcttta tatctgaaat
     3541 ttcccaagtc atatttttag aatacatttt aaatccttct ttatcttcat atataagatt
     3601 acctaaccct gattttggga aatacttatc aacagtatct gcatcttgtg tttttattga
     3661 attaaacata ctatccactt cattttttat cttattatca ttagtacaac cagctaacac
     3721 tagtactgat attgcaaaca ttataataca tataataaat ttcttcatta aattatatcc
     3781 ccctatactt ttttgcacaa atattattct cttttttatc ttttgaaaaa gttgtagtaa
     3841 ttgcattttg tggacaaact tttatacatt caccacacct aatacagtct ggactattta
     3901 catctttata tattggtata tcaagcttac attttgtttg acatgctgta caatttgtac
     3961 atttatcttt atcaatttta aatctatata ttgaaatagg attaaaaaaa ccatagattg
     4021 ctcctaaagg gcatatatac ttgcaaaatg gcctataagt aatgattgac atcaataata
     4081 tcaatagcaa aatacttaac ttccagctaa ataaaaatcc tattgattct cttaaattct
     4141 catttcctaa aataagtggt attcctccca aaagtgttcc tgaagggcaa atccatttac
     4201 aaaaccaagg atttccaccc cctgctacat ttacaatagc aactggcaaa ataattacaa
     4261 acattattaa gactatatac tttagatatt ttagatattt atcaccataa acttttttta
     4321 tctttactgg aaagggtact ttatgtagta aatcttgtac aagtccaaag ggacaaagcc
     4381 aaccacaaac aaatctgcca aaaaaagcac caaatgccat taaaaaacca acaacataaa
     4441 atgagaattt atactctctg cttgataaaa ctgcttgtaa tgaacctatt gggcatgctc
     4501 caagtgcacc tggacatgaa taacaattca atccaggtac acataatttt ttactttctc
     4561 ctccatatat agttccttct aaaaatccat ttacatatcc atttgttaat gctgtaaatg
     4621 caacttgagt aataagtctt atattattat ttactttaga taataacttc attcctcttc
     4681 tcctaaccaa taccaataca ctcaagacaa atcataattg ctttagttaa tactgtattt
     4741 gtttctccac gatatgtacc taatgcaata aatataatac taattgcaat aatagaaatg
     4801 ctttttttat tatctttaat aaaattactc attggctttt accattttta gtctttcatc
     4861 tataattttt ttccaatctt ccttactatt tgcacctaca accttctcac ccacaaagtt
     4921 tcctttcttg tctacaaaat aagttgttgg aaactccata actcttttaa aatcagtttt
     4981 tttaagttct tcagatacag tcaattgttg gtaagttgca tctgactttt tcataatgtc
     5041 tttaactaaa tttttctctt catttgaaag accagttctc atatcagttt tatcaacttc
     5101 tacaactaat ccctttatag ctacattgga attattactc tcatattctt ttgataatgc
     5161 ttcaagctct ggcatttcac ccacacatgg tccacaccaa gtagtccaga cattaataag
     5221 agtaagttct ttatttgaaa atatctcttt tgttacctta tttccatcaa catcctccgt
     5281 atctaaatta ctaaaccatt cactattatt cttgtttttt tctactggct tagattcttt
     5341 tttgctacat ccagaaatca tacctaaaac acaaatacat cctaaaacta ctatgaaaaa
     5401 ccttttcata tctcacactc ctagaattta tatagtatat aaaatcgctg cttgaaataa
     5461 aaat
//
LOCUS       DAELNY010000220         5323 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.223, whole genome shotgun sequence.
ACCESSION   DAELNY010000220 DAELNY010000000
VERSION     DAELNY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.223"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(152..868)
                     /gene="dapD"
                     /locus_tag="KPE55_003354"
     CDS             complement(152..868)
                     /gene="dapD"
                     /locus_tag="KPE55_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG0494389.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1128..1877)
                     /locus_tag="KPE55_003355"
     CDS             complement(1128..1877)
                     /locus_tag="KPE55_003355"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0494390.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(1944..2831)
                     /locus_tag="KPE55_003356"
     CDS             complement(1944..2831)
                     /locus_tag="KPE55_003356"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0494391.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(2948..3949)
                     /locus_tag="KPE55_003357"
     CDS             complement(2948..3949)
                     /locus_tag="KPE55_003357"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG0494392.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTGRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4374..5255)
                     /locus_tag="KPE55_003358"
     CDS             complement(4374..5255)
                     /locus_tag="KPE55_003358"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0494393.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         1735 a          973 c          646 g         1969 t
ORIGIN      
        1 ttgtaaaaaa ataaaatgat tataaataaa agataaaaat attgtaaaaa agagggtgcc
       61 tctggtgaat taaattcaca cagagacacc tttttaatta attttttaaa ttagtatatt
      121 attgtttaat taatagcata gtactattta attagtctaa atttctaagg tcttccataa
      181 gcttagtttt gtcagccgtt ttttcatctt tcatttttat aactttagca ggagaaccag
      241 caacaacggc accagcttct acatcagtag ttacaacagc accagctgca acaacagcac
      301 ctttaccgat tctaacacct tcaagaataa ctgcattagc tcctattaaa acatcatctt
      361 caactataac aggagtagca gaaggtggct ctaaaacacc agcaacaaca gcaccagcac
      421 ctaagtgaac atttttacca agagttcctc tagctccaat aacagcattc atatctacca
      481 tagaaccttc accaataaca gctcctatat ttataacagc tcccatcatt acaacagcat
      541 ttttttctat agtaaccatg tctcttatta ttgcacctgg ctcaattctt gcatgttcac
      601 ttaaagtatt ttttagagga atagcagaat ttcttctatc atattctaca tgagtatctg
      661 ttatatcatt tccatgttta tctaatactt ccattatatc ctcatagtca cctacaaata
      721 tataagaacc attacttcca aataccttaa atttatcagt tgattggaag tgtaaagagt
      781 tagtatttac atatacctta acaggggttt tctttttaac ttcttttata taccttgcta
      841 tttcataagc atcgtttaaa tttaccattt gttaattctc cttgtatttc attatatttc
      901 ttttattata tacttatttg tcaattttag tatatactta cttcttatta tttatatttt
      961 atttagtttt tattgtatag acatagtatt aaataaattt attatatatg tttgatttgt
     1021 attgcgtatt attatatcta tattgcatat gtttgatttg tattgcgtat tattatatct
     1081 atattgcata tgtttaattt atattgtgta ttattatatt tatactatta tcccaacatg
     1141 ctgtccatat tataaaatcc tggtttttgc ttaactaagt atttggcagc agctatagaa
     1201 ccacttgcaa agataccacg agattgagct tgatggctta ttgtaagaac ctcattgtct
     1261 ccagcaaata gagcatcatg ttcgccaaca atatttccac ctcttatagc atgaatacca
     1321 acttcatttg gttgtctttt tgcactacga ccatatctac cataattatt ttcaacgttt
     1381 ggcaaaacct cttttatagc gttagcaatc ataacagctg tgccacttgg ggcatctact
     1441 tttctgttgt gatgtttttc tattatttct atatcaaatc cttctagtaa tttagctgca
     1501 tctttaacca attttactaa tacatttaca ccaagagaca tattatatga ttggaataca
     1561 ggaataactt tagccgcttc acgtattttg ttcatttcat catcattata tccagtagta
     1621 gcaattacta aaggtgtttt agtctttaaa acgtaagata agatattatc taaattagag
     1681 tgatgagaaa aatctataac tacatcagct tcctcaacta tagtactcat atcttcataa
     1741 attttaaggt cgccttcata gctatccaga cctctagctg caacacaaac caattctaat
     1801 tcattatctt ctcttatagt atcggctaaa atcttaccca tttttccatc ataaccagtt
     1861 gctataactt ttaacataat ttcctcctat gattaagggc taacttttga ccaaacttac
     1921 tttttaccat gcattaatct ttactatgca ttaactttta gtccaaagtt aactaattct
     1981 ttctttaata cttctaaatt tgctgggtcc atttctgcta gaggtaatct taaatctcca
     2041 acattgaatc ccaataaatt cattgcagtc ttaactggta taggatttac ttctataaat
     2101 aaagctgcga ttagagcatc cataccaagt tgtaattttc tagaaccttc aatatcgcct
     2161 tcaaagaatt tagctaccaa atcgtgagtt tcttgagggc aaacattagc aagtactgat
     2221 ataactcctt gacctcctaa agataataaa ggaagtatag tatcatcatt accagagtag
     2281 atagcaaagt catctggaac taaactagct atttctgcaa cctgtcctag gtctccacta
     2341 gcttctttta cagcaactat gttatcaatt ttagcaagtt caactataag gctaggactt
     2401 atatttacac aagttcttcc agggacattg taaagtataa ttggaagttt aacactgtta
     2461 gctattgttt caaaatgacg cttaagtcca gctttattag ctttattgta ataaggtgtt
     2521 attattaata gagcatctac tcctaatttt tcagcttctt gacttaagtt aactgagtgc
     2581 atagtatcat tagaaccact tccagctata acaggaactc ttttattaac tttttcaaca
     2641 acatacttaa tagtagcaag ttgttcttca tcagtcatag tattagcttc accagtagta
     2701 ccacaagata ctatagcatc agtaccatta gctatttggt attcaactaa ttcacctaat
     2761 ttgtcaaaat caactttgtt atcctttgta aatggtgtaa ctagagcaac agcagaacct
     2821 ttaaatatca tataaaatca atcccctctt ttttattttt gtgttttata ttacagttac
     2881 ttctaatgct tgtttttgag ttactattaa tattttacaa aattatatat ttatacaagt
     2941 ttgtatatta tactaaatcg tatgataaag ccatttcagc tatttgaatt gcgttagttg
     3001 cagcaccttt tcttatatta tcagcaacaa cccaaagatt tacaccatta tcaacactaa
     3061 aatctcttct tattcttcca acaaatactt catctctatc atcaaattct acagcagttg
     3121 gatattcgtt tttagcagga ttatcaacta ctacaacacc tggagcttct tcaagtgttt
     3181 ttactaaatc atctaactca aatggattgt taaactcaac atttatagat tcactatgac
     3241 cattttttac tggaactctt acagtagttg cagttatttt taaatcgtag ttatcaagta
     3301 ttttcatagt ttcatttacc attttcattt cttctttagt gtatccattt tcagtgaatg
     3361 aatctatatg aggaagacag ttgtaagaga tttgatgagg gtagaatcca gtaggattac
     3421 cgttcattcc tttctctaaa tcttcaacac ctttaacacc agaaccagat acagcttggt
     3481 aagtagagta aacaactctc ttaattttgt atttatcatc aagagcttta aggggaacca
     3541 tagcttgtat agtagagcag ttaggattag ctattatacc cttatgattt tttatggctt
     3601 ctggattaac ctcaggaact actaaaggca catctggatt cattctccat tgactagagt
     3661 tatctactac taagattcca tttgaagcag ctattggagc atacttttca ctaatacttc
     3721 caccagcaga gaataatgct atttgtatgc ctctaccagt aaatgaagtt tcctttaatt
     3781 cttctactat atattcttta ccacaaaaca ttactttaga accagctgat ttagaagaag
     3841 agaataaaaa tagattttct attggaaaat ttctttcttc taatactttt aaaaatgttc
     3901 tacctaccat tccagtagca ccaactattg ctacatttac ttttttcata taaattcacc
     3961 ccaattttaa attattaaaa cattatttta ttttagttat acaagtaaga tatgttaata
     4021 tttttatata tccaaaaact ttaatattat attaacaact ttaattatat acaaaaaacg
     4081 tacaaaggta tactttgtac gtctaattta tcactcaaag taaaccctgt agctcatcac
     4141 ctacaaaaat aagtgacagt attacacatg ttatgtgcaa tcccagaaaa tattattgca
     4201 agaaatattt tcttcggctg taaatccttt ttccaatcat taacacctgc cagctactat
     4261 tggatactct tagttacagc tacctctacc ctaggttaat aggatattaa aatttgatta
     4321 ttctaataat aatacatatt taagaaaaaa tcaacttatt tatataaata ttgctaaaat
     4381 ttaaatccaa aatttgtcaa ttcttgtttt aacacattaa gattagtttc ttccatttca
     4441 gctagaggaa gtcttaaatc accaacatta aaacctaaaa tgttcatggc agttttaaca
     4501 ggaactggat ttacttctat aaataaagca gcaattaaag catccatatc aagttgtaat
     4561 ttttttgagc cttcaatatc accttcaaag aatttagtta ctaaatcatg tgtttcttta
     4621 ggacatatgt tagcaagtac agagataaca ccacttccac ctaatgataa tagtggtagt
     4681 atagtatcat cattaccgga gtatatagca aagtctttag gtactaattt agctatttct
     4741 gcaacttgag ctaagtctcc acttgcttcc ttcacagcaa ctatattatc tattttagca
     4801 agttcagcca caacagatgg ctttatattc actttagttc ttcctggtac gttgtataaa
     4861 ataattggca acttaacgct atttgctatt gtttcaaagt gaagtttaag acctttctca
     4921 ttagtcttat tatagtaagg agttattatt aaaagaccat ccacccctaa tttttcagct
     4981 tcttgactca tatgaacaga caacatagta ttgtttgaac cagtacctgc tataacaggt
     5041 attctcttat taactttttc aactgtatat tttattacag caaaaatttc agattcactc
     5101 atagtagtcg cctcacctgt tgttccacaa acaactagtg catctgtgcc attctcaatg
     5161 tgatattcga ttaattcacc taatttttca aagttgacat tattatcctc tgtaaatggg
     5221 gtaactaaag caacagcaga acctttaaat aacatataat caaatctcct tttttatcta
     5281 aatttaattt tttaagtaca ataataatta tagtacaaat taa
//
LOCUS       DAELNY010000221         5306 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.224, whole genome shotgun sequence.
ACCESSION   DAELNY010000221 DAELNY010000000
VERSION     DAELNY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.224"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            17..1222
                     /locus_tag="KPE55_003359"
     CDS             17..1222
                     /locus_tag="KPE55_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0494394.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1293..1514)
                     /locus_tag="KPE55_003360"
     CDS             complement(1293..1514)
                     /locus_tag="KPE55_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494395.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1655..2254)
                     /gene="recR"
                     /locus_tag="KPE55_003361"
     CDS             complement(1655..2254)
                     /gene="recR"
                     /locus_tag="KPE55_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG0494396.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2267..2617)
                     /locus_tag="KPE55_003362"
     CDS             complement(2267..2617)
                     /locus_tag="KPE55_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG0494397.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2676..4313)
                     /gene="dnaX"
                     /locus_tag="KPE55_003363"
     CDS             complement(2676..4313)
                     /gene="dnaX"
                     /locus_tag="KPE55_003363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG0494398.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4390..4654)
                     /gene="ffs"
                     /locus_tag="KPE55_003364"
     ncRNA           complement(4390..4654)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPE55_003364"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4692..4783)
                     /locus_tag="KPE55_003365"
     tRNA            complement(4692..4783)
                     /locus_tag="KPE55_003365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4747..4749),aa:Ser,seq:gga)
     gene            complement(4784..5239)
                     /locus_tag="KPE55_003366"
     CDS             complement(4784..5239)
                     /locus_tag="KPE55_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG0494399.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1676 a          960 c          623 g         2047 t
ORIGIN      
        1 ttaggaggat tttattatga gcaaaaaagt attaataatg tctgcttcca ctggtggtgg
       61 gcataatagg gctgctctag ctattaaaga agaacttact tccaaaacac ttgatggaga
      121 gccaattgaa tgtgaaataa tagatagttt gaaattagtt aataatacaa tggataaaat
      181 aatttcaaga ggatacgaaa aatctgctat atatactccc aaagcctatg gaagtgtata
      241 tagattatct gaaacaaatc ttttatcaaa aaatgaattt aaagataatc tactaataac
      301 attcatggct aaaaaattta aaaaacttat tagaagcgaa aaaccagact tgattatagg
      361 aacgcatcct tttccaatga ttgctctaag tacattgaaa aaaaacttta atttacataa
      421 taatgaatca aatgcttaca cagagcattt ttataaacat tatacaaata ccattaatgt
      481 tcctccgctt atttctgtat taaccgatta tactactcat tctacatgga tacaaaatga
      541 aattgattac tatattgttg gtcatgaata tgtaaaagaa ttattggtgt ttgatggagt
      601 tgaaccgagt aaaattagaa catttggaat ccctgttgaa aaatcttttc tctctcacag
      661 agataaaggt attgttcttt ctgagttgaa tttatctcct gataaattaa ctgttttact
      721 tatggggggt agctttgggg ctggaaatat aaaagagaca cttgatgaat tactggatac
      781 tgatagagac tttcaaatac ttgtaattac tggtaagaat gaaagcctaa aagaaaaaat
      841 cgaaaaaaaa cttatgtctc gttatcatga taaaaatgta tgtgttcttg gatacactga
      901 taagatgaat gatattttag cttctataga tgtattagta agcaaacctg gtggacttac
      961 tacaactgag gctcttttaa aagatgtacc tatgatagtt ccttattata taccaggtca
     1021 ggaagaagag aatcttgatt tcttatctaa ttgtggtgca gcacttagaa ctacaaaaaa
     1081 atataacttg tcaatattac taaaagtttt aatagatgac cctagcagat tagaaatgct
     1141 aaaaagaaat attaaatcaa taagaaagtc aaattcagcc caaaatatag ctaatttaat
     1201 tttaaatata atttctggtt gataaaataa ataaaatccc tatagtatat atattatcta
     1261 ttagggattt tttattttct aattatttaa tattaaattg gttcataaaa gtcttcttca
     1321 taatcatact gataattttt ataattattt ctaaattcag catttttatt tcgtacattt
     1381 tttttagttt ttttcttatt agaaaaactt atatcctcaa aatcatcata tttaggttta
     1441 tcttttgatt tcattttttt aactcttttt tctttcattg cattatgttc ttctaagttc
     1501 ataattggtt tcatgttgca cccctatcaa aatttttaaa tgatttatac aacaaattat
     1561 acaatacaga agaaatttat tcaataaatc taaatcaaaa ttaatttgaa tttaactcaa
     1621 aaaattacaa aaatcgaggt attaacctcg atttttatat ttctcttctg ccttctagtg
     1681 cttttgatat tgtgacctca tctgcatact ctaaatcgcc tccaacagga agaccatgag
     1741 ctatccgagt gactttgatt cccattggtt ttactaacct agcaatatac atggctgtag
     1801 cttcaccttc aatagtagga tttgttgcca ttattatttc tttgacttcc tgattgccaa
     1861 gtctttggat aagttccttt atttttaaca tatctggtcc aattccatcc attggagaaa
     1921 ttactccatt taatacatgg tactgtcctt taaactctct cgttctctcc atagctgcta
     1981 catctcttgg atcttccaca acacatataa cacttgaatc tctactttta tttgaacaca
     2041 tgctacatgg gtctgtatct gttatattac aacaaattga gcaatatctt atttctcttt
     2101 ttgcatctat aatcgcttta gataaagctt ccacatcatt agtattcata ttaataatat
     2161 gaaaagccaa tctttgagca gtctttcttc ctactccagg aagctttgaa aattcctcta
     2221 taagtctcgt tatgggacct gtataaactt gcataaatac cacctactag aataacccag
     2281 gtatattcat acctccagtt accttactca tttgacttgc ttgtatatca tcaatatttc
     2341 tcaatgcttg atttactgca cttaaaacta aatcttgtag catttctata tcatctggat
     2401 caactacttc tggcttgata gttatatcta taacttcttt tttaccatta acttttacag
     2461 taactgctcc tccaccaact gaagcttcta cttctttact ttctaactct tgttgagctt
     2521 tttgcatatt ttcttgcatt ttttgagcct gctttaacaa gttgttcata tttccaccac
     2581 caggcatcat tccgcctcca aaaccttttt tagccataaa cactattcct cctaaactta
     2641 ttttcatttt attattatat cataatagta ttttattatt tactctcatt ttcatcaata
     2701 ctatctttta tttctaatat ctcttcactc actatgttct ttaatatttc ctcaccaatg
     2761 tcctttttct ctttaacttc aagatttata tctttaactt ctgatgtcag tactattttt
     2821 acattaaaac ttctatttaa aacatctctt attgtttttt ctaaataatt tattgtatct
     2881 tgagagctca gtctagtttt tgcaaaatca aatttatcat caaatataac atacaacata
     2941 ttagaatata cattaaaact ttttacttca cttaagagtg cgtatattgg cattttttta
     3001 tcttttttaa tttgtttaag tatattcttc caagatgact ctataagtat aacgtcctca
     3061 ttttttacat cttcataaac tacattttct tgttttacat ctatttctct atcgacctca
     3121 tactcttttt ctttattatt cccaattttt tcagacttaa agttccccag ttcaatcatt
     3181 ttttctaagt tctcaactct ttttataaga gcttctttac tttcatcaaa cattggttga
     3241 gcaattttca ttatggttat ttccactaaa acccttggat ttgaagatga ctttatatca
     3301 tcctgagtta ttgataatat atttaaaatc cttattaaat catttatatc tatattttta
     3361 gactgaattt taagttgttc tatagtttct tctggtaatg aaattatttc atccaattct
     3421 gatgaaacct tacataccat taaatttcta aaatgatcaa ttaagtcatt tataagattt
     3481 cttatatctt tgccccataa gacaaattca tttaatatct caagagactt tttagtgtcc
     3541 tgctctacta tacattgaga caattcaaat aattgttcta tatttacgct tcccattaat
     3601 tcaacaacat ctttataatc tatcttactt tctccaaaag acatacattg gtctaatata
     3661 ctaagtgcat ctctaagagc tccttgagaa tttctagcta ttaaattaag agctttatca
     3721 tctatatcaa ttccttcatc ttcacaaatc tttttcattc taagggtcat atctttaact
     3781 gtaactcttt taaaatcgta tctttggcat cttgataaaa tggttgcagg tattttgtga
     3841 ggctctgtag ttgctaatat aaaaattaca tatgatggag gttcctctaa ggtctttaat
     3901 aatgcattaa aagctccttg agacaacata tgaacttcat ctattatata tactttatat
     3961 ttagcattgg ctggagaata tttcacactt tctctaagct ctcttatatc atctacacta
     4021 ttatttgaag ctgcatctat ttctacaaca tccattgtat tatcttttaa tacagattcg
     4081 catatctcac actcattaca cggttcttca tttattttat tcaaacagtt tactgctctt
     4141 gaaaaaatct ttgcagtaga tgttttacca gtacctcttg ttccacaaaa caaataagca
     4201 tgtcctatat tatcactata tatttgattc tttaaagtct taataatatg atcttgacca
     4261 ataacatctt caaatttttg tggtctatat gctctatata atgctttatg catcttttta
     4321 cctctcttta gcaaaattaa taaaaaaatc cctcattatc taagggatta gtcttttata
     4381 tattaaatgc tgtacaccat ctatcgactt aattccatag gcgttaccaa aacagttagc
     4441 tcaacttagg cacccccacg gcacacaaaa gtgatcactt accgctgctt ccttccggat
     4501 ctgacggggt tcatgagctt tcattgcgca ggacctaagc atcaacacca cttatttagg
     4561 gcggccctac agaaaataag cctttagatg gaattcaatc ctgctatagc ggattgcagg
     4621 ttacagggca ccgctacctc cccatctagt acagcaaaat tacaaccata tttaattact
     4681 ttcattgttt ctggcggaga gagtgggatt tgaacccacg atacagtttt acccatatac
     4741 tcgctttcca ggcgagcgcc ttcgaccact cgaccatctc tccttaatct ctttttctca
     4801 atgccttaaa aaattcttgc aatatgatcg agcattcttc ttgcaaaaca tcaaaagcta
     4861 cttttacatt attattatta aaataatcta atttaaactc atgctgtttt tctatatatg
     4921 aattttttac atgtcttgtc cctattataa gcttttttat tctagaatta actattgcgc
     4981 cactacacat tatgcaaggt tccattgtaa catataaatc acaatcagtc aacctccaac
     5041 cacctaattt ttctgatgct tgttttatcg ctaaaatttc tgcatgtgcc gtagagtctt
     5101 ttaacgtttc tgtaaggtta tgagctctag ctattatttg attatcttta actataatag
     5161 cacctattgg tgtttctttt ttattatatg ctttatatgc ttcttttaat gcttctttca
     5221 tataaaatga actttccata attcctccca aaacttataa caaacttatc ttatcataat
     5281 tcattttata attcaactta atttta
//
LOCUS       DAELNY010000222         5239 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.225, whole genome shotgun sequence.
ACCESSION   DAELNY010000222 DAELNY010000000
VERSION     DAELNY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.225"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(258..458)
                     /locus_tag="KPE55_003367"
     CDS             complement(258..458)
                     /locus_tag="KPE55_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0494400.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(683..859)
                     /locus_tag="KPE55_003368"
     CDS             complement(683..859)
                     /locus_tag="KPE55_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494401.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTHLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(913..1002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1182..1382)
                     /locus_tag="KPE55_003369"
     CDS             complement(1182..1382)
                     /locus_tag="KPE55_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494402.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1384..1734)
                     /locus_tag="KPE55_003370"
     CDS             complement(1384..1734)
                     /locus_tag="KPE55_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG0494403.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1896..2084
                     /locus_tag="KPE55_003371"
     CDS             1896..2084
                     /locus_tag="KPE55_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0494404.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2561..2731)
                     /locus_tag="KPE55_003372"
     CDS             complement(2561..2731)
                     /locus_tag="KPE55_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494405.1"
                     /translation="MHVYQPKTSEYLLNTSAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3120..3608)
                     /locus_tag="KPE55_003373"
     CDS             complement(3120..3608)
                     /locus_tag="KPE55_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0494406.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4414..4788
                     /locus_tag="KPE55_003374"
     CDS             4414..4788
                     /locus_tag="KPE55_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0494407.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4893..5239
                     /locus_tag="KPE55_003375"
                     /pseudo
     CDS             4893..5239
                     /locus_tag="KPE55_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2103 a          644 c          601 g         1891 t
ORIGIN      
        1 tgtatttatg agatagttga aaattttgat gtgccacaac taaaattatt atattactat
       61 atattctaat taaaagagta cttgtaggtt taagtacata ctttataaat atatttttgt
      121 cagatttatt ttataaaaag tatttagact aagtattatt tttaagtatt ttcttgttga
      181 agttattttt tcctttttta caaaatgcgt aaataaaaaa aagactctta aaagagtctt
      241 tccgaaaaat ctatatatta taatatagtt atattttctg cttgaggacc tctagcgcct
      301 ttaactatat caaagcttac tttttgacct tcttctaatg atttaaatcc tgaagtttgt
      361 atagctgaga aatgagcaaa tacgtcatct ccactttcca ttgatataaa tccaaatcct
      421 ttttcattat taaaccattt tactattcca ttattcatta aaaatactcc tattaaccta
      481 tatcaagata tacttgattt acttgtgtat taccacaaat actattgtat catagattaa
      541 cataaatgtc tatttaatta taatttttag ttaacatttt tatttattct taatatgaaa
      601 ataaatttaa acattttata ttaccaatat attaatgtaa atgtagataa aactaaaaag
      661 ggttttctag aaaaaaatct gtctaattat acttacttac caataccatc acttgatgca
      721 agcaagattc cacaactttt tctccgtact ctaatataaa atcatctaaa tgtgtatcta
      781 taaaatcaca ataatcatat gaactaccaa tataagcagt taacatatgt gtacatttat
      841 ccattttatc tctaaacata atgtatttat ccccctaaaa aactttcatg aaaaacaaaa
      901 agaaaatatc tttttggcaa ctggctgaca taacttattt tataagcgtt agtccctata
      961 gctttgcgtc actaaatttc tctagttttg ccgatttagt tttattctac agattaataa
     1021 tacaatagaa ttagttatta ttcaacatat ctagctgaaa aattaataaa tttatatatg
     1081 aacgttaaat tatctgaaaa aataaaaagc ccctctgtca ggagtgactt aaaaattgaa
     1141 tttaagtttg tatttaaaac acatttttaa taatgcaaaa actaatcaaa aataaacaat
     1201 tcatcaatag gtgtatctac tatttttgat atatctattg ctaatttcaa agaaggatta
     1261 tactttcctg cttctaaacg cataatggtt tctcttctaa ctcctacttt ttctgctaat
     1321 tgttcttgag ttaattcttt taattgtcta tattttttta ggttacaagt gaatttaggc
     1381 atattatttt tcctctaaaa tataaagttt tatagcataa acatttaaag tgattgcata
     1441 tgtaattgat agtaaaacac acattaattc tatattccta gacactatag taatacacca
     1501 taaaatgaat aattctataa tagcaagctg tcctgtaaat gccatagctg ctttttcatc
     1561 ttgtacatac ctttcatctg ctttatcacc atttattttt gctataataa agtttgagaa
     1621 aaaagcaaaa aatccaatat atgtaaggtc agcaacattg ccagatgaaa aatatctaag
     1681 acttaaaaat ccaaggaaac cacaaaaacc cataaaaaat tttttctgaa tcatttttta
     1741 tcctccttag taattttaaa agagatatat ttatatcttt atgatacaaa tatatctctt
     1801 ttatattctt ttgtcaatag aaaatcagta ttatacaata tttgtactgc accataattg
     1861 ttagagcaaa aatctactgc ttgagatgtt tatgtatgac taaatatgat tttaatttca
     1921 aattaaaagt agctaaattt tatttgaata gagaaggaag agatatttta atagtaaaac
     1981 aatatggagt ttatgaccat tctcaagttg aaagatgggt taatatatta ggagagagtg
     2041 aactaaaaat aaaaaaattt tctatactat atggttcaag ttagatgtta taaactatat
     2101 gatgataact aaatatagtg tacaataaat cgctaagtat tttggaataa acaatagcac
     2161 agttatagca acatgaaaat aaaaattttt acatagtggt gttgatgcct tttccaaaat
     2221 gaaaggaaga ccataatgaa taaaaacaaa aacactaaag aaaaaataaa ttaacaagag
     2281 aacaagaatt agaatatgaa aataaatttt taaaagatga gcttgagttt ttaaaaaaga
     2341 tgtgtgcctc agggaagcca cattaaaaaa cagtggtatc attattaatc aaaagaaagt
     2401 tagatgtatt atcaaaaaat tagaacttaa atgcatagca ttttttcata aatcacataa
     2461 atataactcc tataaatgta taattggaaa agtaaaaaat aataaaacaa atagaagatt
     2521 taaaacatca atagcatatc agaaaataac aaatgatatg ctaaaattta taaaatatat
     2581 ctaatgataa aaatggaaaa tcaattataa aaaaggcata tctcgattca ttttttgtta
     2641 tatagtcaaa taagaatact atagatgaaa gatacgattt tgcagatgta ttaagtaagt
     2701 attctgatgt ttttggttga tacacatgca ataaagaagt tgtgtttgca gataaattag
     2761 caaaactata gtataagttg caacaataaa gaaaatgaaa attatcatac taaagcataa
     2821 ggcatgttac aattttatta gataggacat taacatacac taaagtactt tttactataa
     2881 agagtactta tttttttgaa attcattaat gtataaacta tcaagaatat tactcaacat
     2941 aatttaaaaa caacttgaat aggtattttt aaattatgtg atataataaa aaatagaagt
     3001 gtatgccaat acacttctgt agttataact aagcattcta ttcttgagga gtgctttttt
     3061 catttttata accaactctt tttactttaa caataattat cagacctata tatttatttt
     3121 taattgtctg atttattaga taaaaaagtt attatcatat ttttatcatt tggtattttt
     3181 attgaactac cataatcttc ttctaattta tctagaccac ttttacttat ttctccatct
     3241 ataattatgt ttaattctaa gtattcttca ctaccattag atgacactaa agtgtacgaa
     3301 ccattataag gcatgcctaa attttctatt ttatgtgcca aagggtcttc atcaccaata
     3361 ccagagttta ctgttatttt tccatctttt ttagtccacc atattatgtt tccttcagta
     3421 tattttgatt catctgcata gtagctaaga gacgcacttt ttattgcact tatatcagct
     3481 tcaagtttag ctatctttgc tttttctata tttttaaata atgctggaag tgctactatt
     3541 gccaatattc ctattatagc aattactact aataattcca ctagagtaaa tccttttttg
     3601 tttttcattt atttcccctc catataaaat taataatata taaattttta ttttgttgat
     3661 ttctactttc tatattttac catatataga caaaaaaatc actttctatg acaaatatac
     3721 tattatttta caatttatgg aaatgattta aaatgacatt tcttgttaga aaagatataa
     3781 gcaatggatt aaaataaaga aaactacagt catatcaacg ccaataatat tatttaaaag
     3841 cagatgaaaa taaaaagagg gcccagaaaa taatgtctat ttaattatac ttacttacca
     3901 ataccataac ttgatataaa caagattata ctacattttt ttatgttcta gtataaaatc
     3961 atctaaccgt gtatctataa aatcaaaata attatatgaa ctatcagtat aataagtcaa
     4021 aatatgtata catgaataca tattttttaa aagatatata ttattacata cctaaaaaac
     4081 tttcataaaa ataaaaataa aatatgattt tagtaacttg ttaacataac ttataattta
     4141 ttttataagc attagcctat atagctttgt gctactaaat ttctttgatt ctaacgattt
     4201 agtgctattc tacaactaaa ataatacaat agagctattt attgtttggt agcattgttt
     4261 gcaaattaaa caaatttata tatatgaata ttattttacg ttcaaaaaaa cagactaaat
     4321 atgtagaata ttcggtaaaa cttacctcta gtttttgtct taaaaagctc ctattattaa
     4381 cttaagttag aagaattaag gaagtgacat aaattgaaag aagaaatatt aatagaaaaa
     4441 ttattaagag cagaattaat agtaatggag tatttatgga aaaaagacac tctaatgcct
     4501 aaaaaagaaa ttgtaaaaga aataaaacag atttatggat ggcataaaag tacaataaaa
     4561 atactactaa aaaggctagt tgctaaaagg tatttggcta gatatattat aaattctcaa
     4621 tctcattata aaataataga tagtaaagaa tattatactt ttaagaaaaa ggtactaaaa
     4681 tctagtaaaa gcataaaaat aatgcgttca cttaccacta cacataaaaa tgtaagtaaa
     4741 gagaagttag acctattaga tgagtattat agaaacttaa aagaatgata tagtaaaaat
     4801 cgaatgtaat tatttacctt aattcttttg tttgtaaaac tttattgata cataatttaa
     4861 catatacata aattggtaaa ggaatgtata ctatgaaaat tagtaaatta ccagaagcgg
     4921 aattaaaagt tatgagatat atttgggaat cagaaaaggt attaatatca agagaaatca
     4981 ccaagtctat ggaacaaaaa tatgaatgga aagatacaac tatatttaca gtattaaaaa
     5041 gattactaaa gaagagagtt tttagaaacg gagaaaatag ataagtgtac acactataat
     5101 gtattagtaa aaaaaaataa tatcagagat tttaaacaaa agaattttta aagaatacac
     5161 ataaaaattc aatattaagt ttaatgtcag cactatgtag taaggatgat gaagtagatg
     5221 gtgatatttt aaatttatt
//
LOCUS       DAELNY010000223         5209 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.226, whole genome shotgun sequence.
ACCESSION   DAELNY010000223 DAELNY010000000
VERSION     DAELNY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.226"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(152..2431)
                     /locus_tag="KPE55_003376"
     CDS             complement(152..2431)
                     /locus_tag="KPE55_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0494408.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2702..4156)
                     /locus_tag="KPE55_003377"
     CDS             complement(2702..4156)
                     /locus_tag="KPE55_003377"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG0494409.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4320..5189
                     /locus_tag="KPE55_003378"
     CDS             4320..5189
                     /locus_tag="KPE55_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0494410.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1772 a          815 c          558 g         2064 t
ORIGIN      
        1 catattaata aactttgccc aataattcct aaaataatat cctaacaatg atttatgttt
       61 ttataatagt atatttttac ataaaaaagt gaacattgat taacaaagtt cactttttta
      121 taaattttta tgttataatt cacaatttta tttaatttgt ttcttttcta tttaaactat
      181 atctatctaa tattaatttt tcatattctt ctattggcat tggtctagca aataaaaatc
      241 cttgggccat atcacaacct ataaacctta aaaactctgc ttgctctaca gtttcaacac
      301 cttctgaaac aacttttata tcaagttgct ttgccatttg aacaatactt gaaactacaa
      361 tttgactctt ttttgcattt cccactgtta taaagaattg cctatctaat ttcaatacat
      421 caattggcat atctttaagc atatttaaag aagaataacc agaaccaaaa tcatccatag
      481 aaattttaaa tccaacagat ttcaaagttt gcattatttt aaataatatt tttgtattgt
      541 caaatattgc actttctgtc aattcaatct caattaaatt tggttcaatt tcatattctt
      601 ttgttattct ttcaagctca gaaataaagc ctggattctc aagatgtact cttgataagt
      661 ttattgatat aggaaatgtg gggaaatttt cattttccca acgtttaaaa tttttacaaa
      721 cttgggtaaa tacaaacata tccaattctg ttataaaacc atttttctca aaaataggaa
      781 taaatacacc tggacttatt agaccttttt taggatgttg ccaacgtaca agtgcttcag
      841 cacctacaat tttagtagtt gataaatcaa ccttaggctg taaataaact ttaaattgtt
      901 cattttttaa agcatcatgc atttcttgtt caatatcacg ttcttctatg agagtttctt
      961 ttaaattttc agcataaatc tcataaactt ttaaatattt tctttttact gtagtttttg
     1021 caatcatagc tttgtcaaga caggtagaaa tatttgtttc tccctcttca acaaaatata
     1081 cacctatgga tggaattaaa ttgagttcta ctcccaaact tgtttttatc attgacaatt
     1141 ttttacgtat aagttcacat atttctataa ttgactctct attatttttc ttttcaaata
     1201 ttatagcaaa attgtcattt gaaattcttg aaaatacaga ttgctcttta aatatattct
     1261 taattatctc tgctatttcc tttaatactc tatctccttc ttcataccca aacctatcat
     1321 ttactagctt aaacttatct atatcaaaat aaattaaaac tagattcttt ttctcatgag
     1381 aggataaaaa ttttccccct tcaagattaa atttatttga attgccaata cctgtaacgt
     1441 tatctttaaa agcaaattct tccaattttt ccttactctt tttatgtata aatagtatat
     1501 aaattgttaa tgccgtaaag attataatca agactacaca tgctgataat gtaagtttta
     1561 taatagcata tgatttctca gatatagcag ttttaggaac ttcagaaaaa agataccaat
     1621 cattgattcc tattggggca taacttaaat aatatccttt tccaaacata ctataactta
     1681 atgaacctga tttaaagttt tttatatttg acttcatatt gttgatacta gattttgata
     1741 ttgaatcact tctgccaatg taagaaaata aattttcttt agtagagttg gcgtttttac
     1801 tgcttggatt taaaacaata tcaccattac ttctagttat ataagaatat ccttttccat
     1861 taaatgtagg tacagaaagt attttttcat acaacttcgt actatgtgtg gcaaatataa
     1921 ctcctttaac ctcattatct ttataaattg gtacagaata tacattaata ttactattgg
     1981 tatttttatc atcttctatt acatctttta atttgcctgt tataaccact tctccttgca
     2041 ttgattttaa gaaataatct cttttagaaa agtcttctat ataaccatct gtagtgtatg
     2101 ctgtaccatt tggtaaaata actcccattc ttttaaatga actattaata gcttgccttt
     2161 ttagtataga aaggatatta tctacttcaa aagactcttc tccttcaata aatattgaaa
     2221 ttgactctag taactttata tctccattaa tttgtttttt taacacattg acgctttgat
     2281 ttgctatttc tgaaagatat tgattcgttt cttcacctaa tgtcttattc aaccttgaac
     2341 cataaatcaa acaggaaatt gtaattacta agatacttat gacagcaatt atggacaaac
     2401 catacctatt attttttgca tcacttaaca ttatgcttcc tgcctttctc gaatctatgt
     2461 atgtataatc attatcttat gacaattatt gtaaacattt tataataata cttatttttc
     2521 catatttact gtaattatac aaaattataa actttattac aacaataaat taattaatat
     2581 agatattagt gtttatatta ttgcattatc tattttacaa ttatttacat ataaaaaaat
     2641 gtaatatcaa agtgaagctt gatattacat ttttgttctt gtattttgtt taatttttat
     2701 gctacatctt acttaaacac tcatttgcaa cttctttatc atcaaaatga tgcttaactt
     2761 tacctataat ttgataagtt tcatgtccct tacctgttat aattacaaca tcatcctttt
     2821 ttgccatact tatagcttct gctatagctt gttctctatc aacaacaaca tggtaatttt
     2881 cttttttctt gtcaattcct tcaagtatat cttttattat tgcctctggt tcttcagttc
     2941 ttggattatc agaagttata atacataaat cagaatattt ttgtccaatt gctcccatca
     3001 aaggtctctt ttcagtatct ctatctccac cacacccaaa cacagaaatt atttttcctt
     3061 ctgcaaattg ttgagttgtc tttagtacat tttcaagtgc gtcaggagta tgagcatagt
     3121 ctacaattac gcttatacct ttatcatttg gaactgtttc aaaccttcct gatactccat
     3181 tagaaagtct aaggccttct ttatatattg gtttaggtat gccaagtaca tagcaagcag
     3241 ctataactgc taaagtatta tatactgtaa acattcctgg aactggtata aatattactt
     3301 cttcatatga aggtgttatt aatctataag atactccatc cgaatctgat tttatatctc
     3361 tagccataaa atcagcctta gtatcaacac cataagtgta acaaggtaca tttatacctt
     3421 ttatattttc atatatcttc tttccacctt catcatctat gtttattata tttgccatag
     3481 tagtcttaaa aaataatttt tcttttgctt ttctataatc ctctaaatct ttatgaaaat
     3541 ctaaatggtc tggtgttaaa tttgtaaata tgcctagttt aaagtctgtt tcatcaactc
     3601 tatttaatgc caatgaatgt gatgaaactt ccattgcaca gtagtcacat ccattatcca
     3661 acatattatt aaaatgatat tgtaaatcta tactttctgg agttgttgaa ttggaaacaa
     3721 tgtctttttc tccatcaaaa atttttatag ttcctataag tccaacttta ttcttattta
     3781 aagttaatat ctcatttagt atagtagtta tactagtttt tccattagta cctgtaactc
     3841 ctataacgtt aaatttttga gatggatgat tgtaaaaatt atcagctact tttgccatat
     3901 cttttcttgt atcttttacc tttataaatg tacatccttt tattgctaca tcttcttcaa
     3961 caagaaatgc acttgctccc ttttctatgg catcttttat atacttatga ccatctgaca
     4021 caaatccttt tatacatata aataatgtac cttttgtaac ctttcttgaa tcatattgta
     4081 cattattaat atctatattt aattcaccct tgacattgat tatatccaac ccctgtataa
     4141 tatcatttaa tttcataaat gctttcctcc ctttaaactc tttctgctaa aattacacta
     4201 catataatat tatattaaga attttttact atgtgaatgg ttaaaaatgc ttgaaacatt
     4261 gcaaaaaatg ctctataatt ttctataata taactgagga aaggtcttct ataaaaacta
     4321 tggataataa aatcgggtac ttaaacgata actttaaaat atttaatatt agagataaaa
     4381 aagatataca atttgaatac cataatcatg attttaataa gataattata tttataaatg
     4441 gaaatgtaac ttattttgtt gatggtattc cctataaact aaagccttgg gatatactct
     4501 ttatcagcaa taatgaaata cataaaccag ttatagattc aaatgtcttt tatgagagaa
     4561 ttgcaatatg gattagccca gaatttatga aaaaaaatag tgatgctact agtgacttag
     4621 aaaaatgttt taaaatagct acagagaata aatgcaatag attgcgttta aatatacatg
     4681 atattgaatc tattaagagt tttattgagc aaataaatat ttccgaatcc aatactgaat
     4741 ttggaaatga aatattgaga aatgcattat ttatacaact tatgatattc ttaaacagaa
     4801 ttttcctaaa aaatgaaaat atggaatcaa cagaagacgt tttttatgat gaaactatta
     4861 agaaagtatt aaactatata aattcaaata tagatgataa gctttcaata gatagcatat
     4921 cgtctgaatt ttttataagt aagtattatc ttatgagaaa atttaaatct catactggaa
     4981 cgtctataca caattatata gttcaaaaaa gattaatttt ggcaaagtca ctaataacca
     5041 atggtcacct tatgagtgaa gtttgtaata aatgtggatt caacgattat tctagttttg
     5101 taagggcatt taaaaaaaac tacggtgtat ctcctaaaaa ttatataaac aataaaaatc
     5161 ctttagatat tactctttta gatgaataga taatttatgt aaattttat
//
LOCUS       DAELNY010000224         5141 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.227, whole genome shotgun sequence.
ACCESSION   DAELNY010000224 DAELNY010000000
VERSION     DAELNY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.227"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            189..1007
                     /locus_tag="KPE55_003379"
     CDS             189..1007
                     /locus_tag="KPE55_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0494411.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1140..3260)
                     /gene="feoB"
                     /locus_tag="KPE55_003380"
     CDS             complement(1140..3260)
                     /gene="feoB"
                     /locus_tag="KPE55_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0494412.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3286..3513)
                     /locus_tag="KPE55_003381"
     CDS             complement(3286..3513)
                     /locus_tag="KPE55_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0494413.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(3840..4280)
                     /locus_tag="KPE55_003382"
     CDS             complement(3840..4280)
                     /locus_tag="KPE55_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0494414.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4432..>5141
                     /locus_tag="KPE55_003383"
     CDS             4432..>5141
                     /locus_tag="KPE55_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG0494415.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSV"
BASE COUNT         1894 a          702 c          700 g         1845 t
ORIGIN      
        1 aaaagttaag aggggatatc ttttttatga tgggaatgga taaaatattt ataactgaca
       61 tctaactgat atttaagctt taaaatcaaa atcatattga ttttctaaaa taataactac
      121 ttgatttagt aaataataaa gaatatagta actatataga caacaaaaag aaaaggagtg
      181 tataaccctt ggaaaaacag tattttacaa caggtgaatt tgctaaattg tgtggtatat
      241 ctaagcagac attgattttc tatgacaaaa taggtatttt ttctcctgaa tataaagata
      301 aaaataatta tagatattac tcagtttatc aatatgatac acttgatata ttacaatcat
      361 tgagagaaat aggaatgagt ttagaggaaa taaaagagta tatacaaaat agaactcccc
      421 agctttgtgt gaaaatgctt aaagaagaag aaaaaaagat aagagaaaaa ataaaaaaac
      481 taagaaagat aagttctaag atgcaaaata gaataaatat aacagttgaa ggaatttcta
      541 aaatgaatag tgaagaaatt tatatatcca aaaagtttga agaatattta gttctatcaa
      601 gcgatttgag taatatgagt aatagtgatt ttatgatgga attaatagat ttcaccaatt
      661 attgtaaatc taaaaatttg tatcaaggat atgagttggg agtaatagta agtaatgaaa
      721 atataaaaaa tggggactat ttaagtatat caagttttta tttgaagata gataaaaaaa
      781 taaaggataa aaagctatat gtaaagccag aaggaatgta tgcttgcata aaacatattg
      841 gaaaatatga ggattcatat aaatcttacg aaaaattgaa aaaatatata tatgaaaatg
      901 aatataaaat tgttggaaat tcgtatgagg agagtatact tgactttttt tgtgaaagta
      961 atgaagataa ttatatgact gaaatttcaa tccaagtatc cagataagaa ttaaaataga
     1021 gatatattca aatgttaagt aattttactt atcattgaat atatctctat gctttataat
     1081 aaagtatata aatttttaaa tagtttctct aatatatgtc tactgtcagt taatactaat
     1141 tatataaata aattgtaaac tgtagttgtt aaaaaacaaa caacaaaagc aagtatagta
     1201 ggcattaaga atcctaaggc agtccatttt aaactaccag tttctttttt tattgttaaa
     1261 agtgttgttg cacaaggcca atgcagtaaa ctgaacagca taacatttaa agctgtcaaa
     1321 taagtccatc catgttctct aagaacttga cctaaagcac taaagctatc taattctatc
     1381 atagaaccag ttgctaggta agccattaaa agtataggta caactatctc atttgctggg
     1441 aatccaagaa taaaagcaag taatataaaa ccatcaagac caattaattt agctaaaggg
     1501 tctaaaaagt ttgcaacatg tgataatata cttaaatctc ctatatatat atttgcaaat
     1561 atccaagtta tgacaccagc aggtatggct accattacag cccttcctaa aacaaatatt
     1621 gttctatcta ttatagaggt atataaagtt cttcctattt gaggaactct ataaggagga
     1681 agttctaatg taaatgtaga cggaactcct tttaatagcg ttttagaaag agtataagaa
     1741 accaacaatg ttattataac acctagaatt atcagtaaag ttatgcaaag tgcagttgct
     1801 acacttgata cgaaagagtt ggttataact gagctaaaaa atattgtaga tatagctatc
     1861 aaagtaggaa aacgaccatt acaaggaaca aagttgtttg taagtatagc tataagtctt
     1921 tcccttggag agtcaattat cctacaacca ataactccag ctgcattaca tccaaatccc
     1981 atacacatag tcaaacactg ttttccatgt gcacaagctt ttttaaatag gtggtctaag
     2041 ttaaatgcaa ctcttggcaa atatccaaaa tcctcaagta gggtaaataa tggaaaaaat
     2101 attgccatag gaggtagcat tacagagatt acccatgcta atgttctgta taatcctaaa
     2161 accaacatat catttaacca tgatggacaa ttaatactat ttaatatacc cgaaatactt
     2221 ggctcaaatc cgagtaataa attagataat aaagtggatg gatagttagc tccttctata
     2281 gtaatccaca atatagtacc taataataag agcattattg gaagcccaaa tattttagat
     2341 gttaatattt tatctacttt ttcttctcta tcagttgatt ttttagcaac attagaacaa
     2401 cactcatcac tcagtttttt agcataatca tagttgattt tagtaaactc atctcttatt
     2461 ttctgtttat ttatattgtc tggaatcttt ttctttactt cattaatagc atctatagaa
     2521 tctttgtcta tataattact catagattca aatatacttt catcgccatc aataagtcta
     2581 agtcccaacc atctagaatt tatacctggt ataatattat ccaattctgg ttgaattgat
     2641 tttaccacat tctctatatt ttcattgtat ctaacaggtt tattatttaa tttatatttg
     2701 tcaaaagata catcatttaa agtatccaat agttcatcca taccagagcc atttcttgca
     2761 gcagttaaaa taactggaat accaagagaa tcctctaaaa gttttttatc aatagtaatg
     2821 cctttttttc tagcttcatc tattaaatta atacacaaaa taactttatc tgtaagttcc
     2881 atcacttgaa atactaaatt taaatttctt tcaagacatg tagcatcaca tactactatt
     2941 actgcatctg gattaccaaa acatataaaa tcacgagcta caatttcttc ttgtgataag
     3001 gcaaataaag aatatgttcc tggtaaatct attaatgcat attcggtatt tttatattta
     3061 aaattaccac gagctgtagc tacagttttt cctggccagt ttccagtatg ctgtctaagc
     3121 cctgtaagat gattaaatac agtactttta cctgtatttg gattgccagc caatgcaatt
     3181 acaaactggt cttccttatt gtctatatca aacatgtctt ttaaagaact cattttagtt
     3241 gagttatgtg tcaaccccat attaatatcc tcctgctaga aatttttaaa tgtcagaaac
     3301 taatattaag ctactttctt cttgtcgtag agctatcttg ctacctctga ttttatagac
     3361 agttaaatta ttttttggac cttttctaac tacatctata acagctcctc tagtaagacc
     3421 aagcgctaac attctttctc ttaaattacc actagataat atatcttcaa ctttgacagt
     3481 ttttttcact tgaatatcgt taaggttttt cataatagca cctccatgct tgaaatttag
     3541 cctgggctaa tcttcttatt ttaaactatg cattatatta ttaccttgtt acttttacaa
     3601 aaaaaagata ggcaagatgc ctatcataca aagttgggta taattcttac ttttggggaa
     3661 agtagaatat agaataaaaa ttaacacttt gaatccatat aattattgaa ttttgataat
     3721 aaaaaagtca gaaagcaaaa gccttctgac tctatgtaag gtattttcaa atacctaaat
     3781 atcaattcaa tatttaattt atacttaaat tatatattaa caatattagc ttgtcaacac
     3841 tataatctca ttttaatatg ttcaatacct gcttcgtcgt atacttctga gatttcttta
     3901 aaaccacaag ataagtaaag gttctttata taagtttgtg ctgataaggt tatattattt
     3961 tcgtgtaagt taacttttat aaaatctata gcacaatcca tcatttgttt tgctaatcct
     4021 tttcttctat gactagaaag aactaaaact ctaccaatgg aaacatcatt ataagagctg
     4081 tattctttag gaattattct agcgtaagca caaatcaatc cattttcttt taaaaatatg
     4141 tgataagagc ttttatctat atcatcataa tcgtttaatg aaaatatctt ctgttcacaa
     4201 gcaaatacct catatcttga ttttgctatt tcataaaatt catctaaact caaatcatta
     4261 aagtgtttaa ttttaaacat agtaattacc tcctagcatc ttaaaaaact gaatattaag
     4321 atacattata ccatttttaa cataaatacc tttattaaaa tttatattta ttaaaaatat
     4381 ttttaaaaag tgatatacta tattaaaaac aaaactttag ttagaaaatt tatgatttat
     4441 ggaggcagtg ttttgaatac tactaaaaaa ttaactgaag cagctttatt gtcatcttta
     4501 ttcatagtag taacaattat agctgtgagt actggatttg gatatgctat ttacttagat
     4561 tttatagtgc cagtattttt ttgtataatc tgcttgaaat gtgatttaaa gtataccatt
     4621 ttatctggaa ttacttcgct tttaataatt tctctggtac ttggaaattt aggaactgct
     4681 atttgggcaa gtcagagtgt attacttggt ataatgtgtg gctatctaat aaataagcct
     4741 acaatggtta tggatgattt agtatatggt tcagtatttg gggttattgt aatggttttc
     4801 attgatatat atgcttcaac attaataggt tatagtttta tgaaagaatt tcaaggatat
     4861 agcaaatgga tttattttaa tgggtataca aattttattt attatttaat gatagcattg
     4921 tttccttttg gtatggtgtt ttgcatatat ttattaagtt taattttagg gaataaattg
     4981 catattttag attctaattc tttaaaaaaa tttttaatat ttaagaattt tagaaattta
     5041 aaccaatttt tatgttgctc aaaaaaagct ttttacatat gtgtttctta tctagccatt
     5101 tttgaagttc taaaactgtt gaatgttaaa gttgattctg t
//
LOCUS       DAELNY010000225         5019 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.228, whole genome shotgun sequence.
ACCESSION   DAELNY010000225 DAELNY010000000
VERSION     DAELNY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.228"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            400..1701
                     /locus_tag="KPE55_003384"
     CDS             400..1701
                     /locus_tag="KPE55_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0494416.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1777..2052
                     /locus_tag="KPE55_003385"
     CDS             1777..2052
                     /locus_tag="KPE55_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0494417.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3878..4108
                     /locus_tag="KPE55_003386"
     CDS             3878..4108
                     /locus_tag="KPE55_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0494418.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            4101..4682
                     /locus_tag="KPE55_003387"
     CDS             4101..4682
                     /locus_tag="KPE55_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0494419.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
BASE COUNT         1973 a          411 c          976 g         1659 t
ORIGIN      
        1 ttactatcag gtagtgaatt atattgaacg atatattaat aattggcagt aaattatatt
       61 aaataagata ttaatatcga gtaatgaatt atattgaacg atatatcaat aattggcagt
      121 aagttatatc aaataagata ttactatcga gtaatgaatt atattgaaca atatattaat
      181 aattggcagt aagttatatc aaataagata ttgctattat gtagtaaatt atataaaaca
      241 atgtatcaga ataaatagta tattaataat ttgtagtata tcatgacgaa taatacagtc
      301 ataacaaata gtgagtcata acaaataatg tatttataat tgataatgta tttaaaaatc
      361 aaaatataaa aacttataag gaaagagaga ttaatttata tgggtaaatc aaaagatgtt
      421 gaaatagaga agtctatttt aggcacaatt cttttagaca ataaattaag ctataagcta
      481 gatgaattaa atgaaaacat gtttatgaat gatatatgtc ttgagatatt taaaattatg
      541 aaagaactta aaaaagaaaa catagtgatt gatgtggcca ctgtgaaatc aaagatagat
      601 agaaaatctc tagcaataaa aacgagtgat gtgactaatc taattacttg gggtcagaat
      661 tttggtcttg atggtcatat taaaatttta aaagaaaatt tagctagaag aagcataaat
      721 caaaactgcc aaaacctttt acacagccta aaccttggag aaaatatcga tacttgtatt
      781 tacaaatttg aaagtaatat aaaggagatt ttggacaagg atacttatga aaatgatgat
      841 gtaaatagta tagcagggaa ggtgcttgat tttttagaaa acaaaaaaga tattggattt
      901 aaatttggta taaaattact tgataccact ataggaggtc tttttaaagg agagctaacc
      961 acaatagcag ctaaatcggg ggttgggaag actgctttgg cacttcaaat aatgttaaat
     1021 tcatttaagc aaggtaaaaa aactttattt ataagtagag aaatgaccag tgagcaagta
     1081 tttatgagaa atatatgcag agtaactggt gtaagtacaa ggaatatgaa atctaaagaa
     1141 atagatgaaa atgattggaa acttattgta aatgctatag gtgatttaag cgaaaataac
     1201 ttaatttata tcaatgataa gatagataca attagtgcaa ttagaaagag gataaggcag
     1261 gtaaagccag atttattaat agtggattat gtgcaactcc ttacatctca gaaaagcatg
     1321 gataagagag aaagggaagt agctactttt tcgagagaac ttaaaaatat gactttagat
     1381 tttaatatac ctgtaatcca attaagtcag ttaaatgatg aaatgaaaga ttcaagacct
     1441 tatggagata gacctatgag ggatagcaaa gccatctacc acgatagcaa caatgtggtt
     1501 tatatacatc aattaaaggg gagtgattat gaagaagcag tgagagatat tggagaaagt
     1561 gaggaggcag ttagagcatc agaatacagg ggaattaaga tggttgattt aatagttgcg
     1621 aagtgtagag atggacagac taggcataag catttttgtt actttgggga taagttacat
     1681 tttcaagaac taaattatta ggtcaataaa gtagtaataa caagcttatt aaaagtcggg
     1741 aaatagcaaa tagattataa aatatgaggt gaaaatatgt tggaattaag aaaaattata
     1801 gaattacttg aagaacacaa gatagaagta agtattccaa aggagttaca taagtatagt
     1861 ttattggaat accttgaatt gtgtcaacaa agtgggggaa catattttta tattaaaaca
     1921 ctggaagggg ctttaaaaga ggctagaaat gagcttatga ttaaattgtt taatgggaaa
     1981 aattatatgg aattagcgag gatgtttgaa atgagtgtta ttaatgtcag gcatattgtt
     2041 agaaaaaaat agatattttt ggagaataca agtgtgatat acttctgaaa tagaagttat
     2101 tatagttgaa ataaaattgt ggttaataaa ttaaagttgt gtataagttt aagattatag
     2161 aggagtataa gtgtgattaa caacatgatt ttgtgaataa aattgaggat atatatgagt
     2221 taaatgtgtg attaataagg taaggttgtg tataagatta tgagtattta aaaggagggg
     2281 ctataattaa tagtgtagag ttgtgtgttt gcttaagatt atataataat ttaatagatt
     2341 aaactatact aataaggtaa aatcgtgtgt ttagttaaag cttattataa aatcttgata
     2401 tgtgattaat gatgcaatat gtgaataaag ttaagattat aatagattta acctgtggct
     2461 aatagaataa aattgtgaat aaagttaaga ttataatagg tttaacctgt gattaataga
     2521 gtaagagtgt aaatagagat aaacttcaat atgagcttaa cctatgatta atagagtaag
     2581 agtgtaaata gagataaact tcaatatgag cttaacctat gattaataga gtaagagtgt
     2641 aaatagagat aaacttcaat atgagcttaa cctatgatta atagagtaag agtgtaaata
     2701 gagataaact tcaatatgag cttaacctat gattaataga gtaagagtgt aaatagagat
     2761 aaacttcaat atgggtttaa cttgtggtta atagagtaag gtgtaactaa agataaactt
     2821 caatatgggt ttaacttgtg attaatagag taaggtgcaa ctaaagataa acttcaatat
     2881 gagcttaacc tatgattaat agagtaagaa tgtaaataga gataaacttc aatatgggct
     2941 taacttgtgg ttaatagagt aagagtgtaa ctaaagataa acttcaatat gagcttaacc
     3001 tatgattaat agagtaagag tgtaaataga gataaacttc aatatgggtt taacttgtga
     3061 ttaatagagt agggtgtaac taaagataaa cttcaatatg agtttaactt gtgattaata
     3121 gagtaaggtg caactaaaga taaacttcaa tatgagctta acctatgatt aatagagtaa
     3181 gaatgtaaat agagataaac ttcaatatgg gcttaacttg tggttaatag agtaagagtg
     3241 taactaaaga taaacttcaa tatgggttta acttgtggtt aatagagtaa gaatgtaaat
     3301 agagataaac ttcaatatga gcttaacttg tgattaatag agtaagagtg taaatagaga
     3361 taaacttcaa tatgggatta acttgtgatt aatagagtaa gagtgtaact aaagataaac
     3421 ttcaatatga gcttaaccta tgattaatag agtaagaatg taaatagaga taaacttcaa
     3481 tatgggttta acctgtggtt aatagagtag ggtgtaacta aagataaact tcaatatgag
     3541 tttaacttgt ggctaataga ataagattgt gaataaagtt atagttttag atgggtatta
     3601 acctgttact aatagaatat gattaggtgt aaaattatag agctgtataa gaaaatatta
     3661 tagatatatt aaatattaga taataaatat ttatatagat acacaaaaga aaaggcgata
     3721 tgaagctaaa cttaatgact tcatatcgtt ttttatttga aactcctttg tatttagagt
     3781 gtgattttta atattgaggt tttatgtgat taagtcacag ataaaaagta agtatcttga
     3841 tataattctt atagattaat aatataggga ggttattatg tctaataaaa aaaagaagta
     3901 tgcaaatatt ttagcaaatg agtgcgttgc ttgtggtagc tgtataaaag catgtccaag
     3961 aagtgctatc tcagttccat gtggcattag tgcaaaaatt aatagagatt tatgtgttgg
     4021 atgtgggatt tgtgaaaaga tatgtccagc atctgtaatt gagattataa caatattaaa
     4081 agaggaagaa ggtaagtgtc atgaataata aaaaacattg gtatgactat ttatggattt
     4141 tttcttcaat atatttgata ttgggatttg taaatattat tttcgcttgg attgggctaa
     4201 tttgtttctt tgttccacta gctatttcta ttgttaaggg aaataaagct tactgtaata
     4261 agtattgtgg aagagggcaa ctgtttaatt tgcttggaaa taaattgaag ctgtcaagaa
     4321 atagagatat tcctaaattt attagaagta agtggtttag atatgggttt ttgacatttt
     4381 ttatggtaat gtttgctaat atgctttttt caacttattt agtgtttgaa ggaagtagaa
     4441 attttaaaga agtagtgact ttgttgtgga ctataaaaat gccttggcaa tgggcgtatc
     4501 atggaacttt agtttcacct tgggtggtac agtttgcatt tggtttttat agtgttatgc
     4561 ttacatcaac tgttcttggt ttgattacta tgatattgtt taaacctcgt tcatggtgtg
     4621 tttattgccc tatgggtact atgactcaga tgatatgtaa ggcaaagagt aagtctgtat
     4681 agaaaggcct tatgtgatat ttacagtttt gttcaagtag agatgagtag taggtttgaa
     4741 tatatatgac aaatatatga gtataggggc atagatttag tttgtgtaag gcaaactgat
     4801 ttatgcttct tttttatgtc ttagattatt ggctaagtag aaacaatgaa tttagatatt
     4861 aagaattatt cagttatttg aagaaaatag aataataatt aacattatat gaatggatat
     4921 tcattacata taagcagtta atagcaagat ttatttaata tgaaataggt caagtattta
     4981 aactaaataa ggtcatgaca attaatataa gtatagata
//
LOCUS       DAELNY010000226         4909 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.229, whole genome shotgun sequence.
ACCESSION   DAELNY010000226 DAELNY010000000
VERSION     DAELNY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.229"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1123
                     /locus_tag="KPE55_003388"
     CDS             92..1123
                     /locus_tag="KPE55_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0494420.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLTTRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1256..2554
                     /locus_tag="KPE55_003389"
     CDS             1256..2554
                     /locus_tag="KPE55_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG0494421.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2668..2913)
                     /locus_tag="KPE55_003390"
     CDS             complement(2668..2913)
                     /locus_tag="KPE55_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0494422.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3061..3927
                     /locus_tag="KPE55_003391"
     CDS             3061..3927
                     /locus_tag="KPE55_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG0494423.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3931..4467)
                     /locus_tag="KPE55_003392"
     CDS             complement(3931..4467)
                     /locus_tag="KPE55_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG0494424.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4658..4861)
                     /locus_tag="KPE55_003393"
     CDS             complement(4658..4861)
                     /locus_tag="KPE55_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0494425.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
BASE COUNT         1955 a          531 c          785 g         1638 t
ORIGIN      
        1 gcaagaaata tagcatttaa aagataaatt gtgcaactta aatcaaagtt taagatgaac
       61 tattaacata gatatattaa aaacttttgt aatggagaat atatgtgata tgaataataa
      121 agttaaggtt gcagtattag atacaggaat agacaaagaa catgattact taaaagataa
      181 tttagttgga ggaattgctt ttgaatgtat gcatgattat atctttatat cagataaatt
      241 tgatgatgaa gatggtcatg gaactgcttg tgcatcaata ataaagaaag agtatgaaga
      301 tgtagagtta tttgtaataa aaattttgag aaaagatggt attacaaata taaaagttct
      361 tgaagaagca ttaagatacc ttttagacac taacataaga ttaattaatt taagtttatc
      421 agtcatagga gtagaaagtg ttaagggctt atttgaagtt tgttatgaat tgtttagaaa
      481 aggaaaaata atagtatgtt cactcgctaa tgattttgac ttaagttatc cagctatgtt
      541 taacaatgtt ataggtgtaa gaggatttac tttagatgga gatgattcat tttggtataa
      601 taaaaaatat gatgttcaat gtgttatgga tagcaattca tatataagtt gtgatataaa
      661 taattcctat agattaccac caaaatgtaa tagttattta tcagcaaagc ttactggtaa
      721 aatagctaag atattatcag aaaaaccagg cataacaata tctgatttaa atgataagct
      781 tgaatcatta acaactagaa atcattggga tagttgtgat tttgatgagt gtagtagaat
      841 tgtaaacttt aaattggatt tgtatgacaa agaaaatgct ctttttatga aagttgctga
      901 cgttgttagg gattgcttag atattgaaga gaataatgaa aaactcttta agggtagcct
      961 ttttaataag gagataggct tggtttatga taattgtttt aatctgttga aaaagctaga
     1021 gcatagattt gacataaagt ttaattatat ggacatctca aaatatgact tagtttcaat
     1081 atatacactg actgaattag tggaaagata taaaaactta taaataaaga tttgtagcta
     1141 aatttaatgt agaaggtatt aaattatatg tattctttgt cggtagatta aaagtaaaaa
     1201 atatactata attagatagc ataagagaaa atgacaatac agggggagtt agataatgaa
     1261 atatgaagaa gcattagagt acatatcaca aactaataaa ttcggtataa gattaggatt
     1321 agaaaatata ggaaagttat tagaattatt aggaaatcca caagaaactt tgaatataat
     1381 acatgtagct ggaactaatg gaaaaggttc tgtgtgttct tttgtttcta atatactaag
     1441 agagtgtgga tataaagttg gtttatatac atctccatat ttagaaactt ttactgagcg
     1501 tataagagta aatggacaaa atataccaca ggatgatgtt gctagaataa ttgagttaat
     1561 aaaagaaaaa atagagatta tggtaaaaga aggctatgca tatccgacag aatttgaagt
     1621 ggttactgca atggcatttt attactattc agaacaaaag gtagactttg tggcattaga
     1681 agttggttta ggtggcagat atgatgctac aaatataata acaaaatcac ttgtaagtgt
     1741 gatagtatct ataagtttgg accatacagg tatacttgga gatacaatag aaaaaatagc
     1801 gtacgaaaag gctggaataa taaaggaaaa tggagtagta ctagtatatg accagactga
     1861 tgaagcaaag gatgttataa agtcagtttg taaagagaaa aaagctaaat atatagaagt
     1921 tgattttgat gatataaaca taaaaaaatc agatataaat tctcaaatat acgattgtac
     1981 tgtaatgaaa gaaacttata gagaccttga aataaaattg ataggcgaac atcaaataaa
     2041 caattctatc ttggcaatta gtgtaataaa atatctaaaa gacataaata aattagcaaa
     2101 tataaatgaa gaatctataa gaaaaggact tataaatact aaatggccag gaagaataga
     2161 aaagataaaa gaaaatccaa tttttataat agatggtgca cataatgaag atggagcaaa
     2221 atctttggca aaagcacttg ataagaattt taaaggtaga aaattaacct tattaatagg
     2281 tatgttagaa gataaagata ttgatagtgt tttagagata ttgttgccac attttaataa
     2341 ggtaataaca acaacaccaa ataacccaag ggccataaat tcagatatat taagagaaaa
     2401 agtattaaaa tatgtagatg atgtaacatc aaagcatgag atagaggatg cagtaaatta
     2461 tacattagaa acatcaagtg aggatgatat tataataagt gcaggttcat tatatatgat
     2521 tggtacagtg agaactttag taaagaaatt ataattttat aaaataagta tacaaataaa
     2581 aatataaaaa agagttgtct aaaaaatgga caactctttt tatataaaat aagtttttaa
     2641 taattttact gatgtttata taagtaatta tatagattga cgtatagctt tagataattg
     2701 gtctgggcaa gaagtacctt ttccaccaca gtcaatacca tttaatttat cagcaacttc
     2761 aagagcattt tggccaatta ttaagcttct aagtcctgat aaattaccat tacatcctcc
     2821 tataaactca gcatcaacaa tcacattatt ttcatcaact tcaaaaatca tttctctaca
     2881 acatacacca cttggattaa atgttacttt cattaaaaag acctccatta aatattattt
     2941 ctttttttaa cattaataat aataccaaat atatgcgtaa taatgcaact ataaagcata
     3001 atacaagcta atgaaataat atattattgt agtatatgta ttgtagagag gaggggttat
     3061 atgaaaatct atatttctaa ctacttatca aagagtatta gcatagttga ttattctaca
     3121 cttgaactag aaaaagaaat agtattagaa gataatatat atccacacca tttttgcata
     3181 gaaaaagaaa aaaatttaat atacatacct agttcaagta atgggatact ctatgtctta
     3241 gatttaagta atgataaaat aattgatact gtctctatag gaggaagtct tagtcaagtt
     3301 gtacttagcg ataatgaatt gtttgtggca aatgaagatt ctaatagtat atatgtacta
     3361 gataaaaaca cattaaatcc aataggaatt attggagttg ataatatgcc acatggattt
     3421 gactttgata gagaaagcaa aaagttatat gtaccgtgta taaactcgat agtatgtata
     3481 gatacaatta ataaaaatat acaaaagaaa ataaatatgg actttagagc ttggcatata
     3541 gaacttgata aacaaaagaa agaaatgtat atatcaacac ttgatggaaa gcttataata
     3601 atagatgaag taagcatgga tataaaaaag gtattatatg aatttttatt accagtagaa
     3661 ataagattta attatagtgg caaaaaagtt tatgttgctg atttgggtta taataatgta
     3721 agaattttag attatacaac gagtagatac attggaaata ttgaaataaa tgggattccc
     3781 caaggattag aaatatctaa agatgaaaag ttactatttg tatcagatac acaagaaaat
     3841 tcagtaaaag tttatgaaac tgcaaataac aaattaataa aagaaatcaa ggtaggaaaa
     3901 gagcctacaa ctataatttg tttgtaggct ttaatgtatg tctcttataa gtaaatcgat
     3961 aacttcggca tacatataag atgcattatt tttccagtta ttgcaaataa gttttgcttg
     4021 tttctctgat gaaacattta gatttaaatc tattaagtta gactgatttt ctatgactct
     4081 aaggttgaca ataaattcat tatcactttg ttttacaaaa ctagacttta cttgagtatc
     4141 tgctagtaga ttttctttgt tagaatctat atacttgtct attttttcag taacactagt
     4201 aggtattcga ttaaagaagt attctagact ttctacgcct ctttgagtaa gagcataata
     4261 ttctctgtca gagtctttat atgtagtcaa aaacttagat tccataagct gagataaaag
     4321 ctgttgtaat gaaaaataat tcatcatctc agtctctaat actacttgag ttatttgaga
     4381 attagttaaa tccattttaa ttttttctaa tatatataaa attaaaagtt tgtgataggc
     4441 taattcttca gacgagtttt caaacataaa tatcatcctc cgtcaaacta tttctatata
     4501 taatatatta aactaatttg tacaaaatta aaagaacaat ttgtattgta ttattaatct
     4561 ttaataattc atataaaaac aattttataa ataaatatag agaaacaggg ctgctaatta
     4621 gcagccctgt tgatacccag gtgtcaaaat ttactattta ttttccagcc atttgtcttt
     4681 cagccatttc aactaatttc ttagtcatat atccaccaac atatccattt tctctagctg
     4741 ttaagttacc tttgtctatg ttttcatagt tagataaacc tatttcatta gctatttcta
     4801 atttcatttg gtttaaagca gcttttgctt ctggaactac tgttctgttt gaatttgaca
     4861 tagtaaattt cctcctcaaa taattagttt atgatgtcta acaacactt
//
LOCUS       DAELNY010000227         4892 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.230, whole genome shotgun sequence.
ACCESSION   DAELNY010000227 DAELNY010000000
VERSION     DAELNY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.230"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            30..641
                     /locus_tag="KPE55_003394"
     CDS             30..641
                     /locus_tag="KPE55_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG0494426.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            939..1277
                     /locus_tag="KPE55_003395"
     CDS             939..1277
                     /locus_tag="KPE55_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG0494427.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1684..3120)
                     /gene="miaB"
                     /locus_tag="KPE55_003396"
     CDS             complement(1684..3120)
                     /gene="miaB"
                     /locus_tag="KPE55_003396"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG0494428.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3368..4840)
                     /locus_tag="KPE55_003397"
     CDS             complement(3368..4840)
                     /locus_tag="KPE55_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG0494429.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPT
                     ETSARLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSI
                     KKVNTVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASM
                     STTLVNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGP
                     MVLVNDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTI
                     KDGMYKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKN
                     GQYIKIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRN
                     DSLGNAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT         1641 a          750 c          541 g         1960 t
ORIGIN      
        1 ttttaataat attattttag gaggtaaaaa tgataaacga tattaaaaat aaattcaaaa
       61 attacaaacc atatataaat ggatacaaga atatgaagag agcatctgtt ttaattccaa
      121 tagtagaaat aaataacaca cactatatat tatttgaagt tcgctcaaaa aatttgaagc
      181 atcaacctag tgaaatttct tttccaggtg gtaaaataga atcaggtgag actccatatg
      241 aagctgttat tagagaaact tgtgaggaac ttggaacttt tagtgacaat atagaagtaa
      301 tatcaagtct tgacctcctt ataacaccag taaagtttat catacatcca tacttaggat
      361 atataaaaaa tattaataat ttaaatataa acaaggatga agttgaccat acttttttgg
      421 ttcctataaa atatctttta gagaatcctc caaatactta tagtaatact gtaaaagttt
      481 ttccagacga aaatctacca tatggttcaa ttccaaacca aaaagattat aaatttgaaa
      541 gtggaagtta tcctattctg ttttataaat ataaagatta tgtaatttgg ggaatgacag
      601 caaaaatact agaaaacttt ttaaattttt tatcagaata acactcataa taaatcatca
      661 aaacttttac ataagaaatt tataaaagtt tctaaattta acaattctaa aatatcaata
      721 actatatttt gtagcacttt tttattttat tattgtttga atatttgatg gttttcaatg
      781 caatttcaac ataaatacta tgtattagtg tatactgttg atgaattaaa gtatgataat
      841 ctatttaata aagtatgaag taaatgattt taatgtgcct ttatggtaaa attactaata
      901 agtaataaaa ttaattaaaa tatatggagg tgcttataat gtttgctgta ggatttgttg
      961 ttataattgc acttgccttt ttcattttaa gtgtactatt atttaatgga aaagccagta
     1021 tgctactagc aggttataac tctatgagta agaaagaaaa agcacgatac gataaaaaaa
     1081 aattatgtag aagttctagc attgttactt ttattgtctc tataatgcta tttattatgg
     1141 cgtttttggg atatagaata gagagtggtc aaatggacga aaaaaaaatg gttccctttt
     1201 ctataatatt tataatagtc atactttcca gtgtattttt taatatcctc tactctaaca
     1261 aaaaatgtaa aaaataaata gttgtaagtt gagcagttaa ttataaaaga ttgactgctt
     1321 tttcatgtat attgactact cttattaaat attttaacat ttaaaaatct atgtaaaaat
     1381 aatgaatata gtacaaactt ctaataatga ttttaccttt gtggttacag tccactatat
     1441 ataatttaat atgataacag ggaattacta atgacaacta ggtaagcttt tataaaatat
     1501 aataaaacaa aaatagatgt acaatgaaat tatatcttat atcaaattta tttttattaa
     1561 tagcaaaaac ttacattagc ataacatatg acaaattcgc ttaaaataaa aatttttaat
     1621 ataatttgtt ctatagtaca tctattttga ttagtttaca taattttatt attttgtaca
     1681 atattaatta tttactaata tcccatttaa tgaaaaagtt ctaggttcag taatttttac
     1741 cataactaat ttaccaatta aatcatcatt tccaccttcg aaatttacga gtttattttg
     1801 tctagttcta ccagcaaact tattttcatt atttttactt cttccttcaa ctagaacttc
     1861 aactattcta tctttatatc catcatttat ttctgcagaa atcctattta cagcttctaa
     1921 caccctatta aatctatcat gctttatatc ttctggtatt tgattttcca ttttagcagc
     1981 aggagttccc tgtcttttag aataaataaa tgtaaaagct gaatcatatc taactttttc
     2041 aaccacatct aaagtgtcca ataaatcttc ttctgtttct cctgggaaac caatcattaa
     2101 gtctgtagaa aaagcaatac ctggaacttc ctttttagct ttttctatta tttctaaata
     2161 atattccttt gtataatgtc tattcatttt tttcaaaagg ctactgcttc cacattgtat
     2221 tggtagatgt aaaaactcac acaccttatc acaatctcta attgcgtata ttacttcatc
     2281 agatatatct tttggatgag atgtcataaa tcgaaccctt tcaattcctt ctatatcatt
     2341 cacttttctt aaaagttctg agaatgaaac tggattttca agtgtcttac cataagaatc
     2401 tacattttgt ccaagtaaag tgatttcttt cgttccattt gcaacaagct cttttatctc
     2461 gttaattata tcttcaggag ttctacttct ttctcttcct cttgtgtatg ggactataca
     2521 atatgtgcag aaattattgc atccatacat tatatttaca aatgctttta attcaaattt
     2581 tctattactt ctaagacctt ctattacttc tccatccaca tcccatacat ccactaacat
     2641 tttgtcagaa tttattgatt ctgtaagtaa ttgcggaaat ttatatagat tatgtgttcc
     2701 aaatataagg tctacatgtt tatatttttt tcttaattcc tctacaacat gtggttgttg
     2761 catcatacat ccacatacag ctattttcat atctggattt tttcctttta gatgttttaa
     2821 ttgtcctaag ttaccataca cttttagctc tgcattttct ctaactgcac aggtgttata
     2881 tattattaaa tcactttctt caaccatcat tgacatttgg tatcccatct cttctaacat
     2941 tgaacataat ttttctgaat catgttcatt catttgacac ccgaaggttt gagtaaacac
     3001 gagttttctc tttccagttc tgttgaagta ttcttcattt tgtctgtgta tttgttctat
     3061 atatttatct tgttcttgta ttttctctat aggtattgtt acttgttttc ttttgctcac
     3121 gtctattcct cctagttaat ttcttatatt acacataacc ttaattatat caaataagaa
     3181 aaatctttta aagatattat tatttttcta gttgtgtttt aatagtaaat ctaatgattt
     3241 atatatacta tatttctaaa atataaaatc atataattat tataataata aaaagatgtt
     3301 ttaaattaaa acttaaaaca cctttcctta aataaaatat atgatttact attaaataca
     3361 attacatcta ttcattttca attaaataac aatcatttaa tattaaatat tgatcttcct
     3421 caaccgttat atatcttctt cctgaaaaag tatcattagt aactattccc tctgcattac
     3481 ctaagctatc atttctaact tcataatatg catcagaaga atttgacatt ataatatatt
     3541 ctccagcagg aatttctaat ccaattttat acataccatt tccaaactta ccattcttgg
     3601 ccttctgggc tttagcatac tttgctaatg tcattgttga atcctcaatt ttaatatatt
     3661 gaccattctt taaggtgata tatctagtcc cactaaaaat atcatttgaa agtatactat
     3721 cagcattacc tgtactatca cttgtaactt cataataaga agcataactt cctgagtttg
     3781 aagttattaa gtattcacct gctgagatat cttttccaac cttatacatt ccatctttta
     3841 tagtctttgt agtaggttta acatttttta caaccccagt attaatatct ccaattccat
     3901 ttgttatatt taaacactgt tgtactatat ttgaatctat atatcctata gaagttatct
     3961 tcttagcatt ttttaaaatg cttttattgc taccatcgtt aactaaaacc attggaccat
     4021 gttttgataa agaagatcct acaagtgcat ttgttaattc ataagctcca gctatataaa
     4081 actctctagc atccttataa aacttattta ttattgcttt attagtttca aaacgagtac
     4141 tcccccctag tctagtagat ttagtattgt ttactaaagt ggtactcatt gaagctgttc
     4201 ctcctattac ataagacttt aaaccactag ttgaaaaagg tatactttgt ccattagtaa
     4261 gaattattgg tgctttatct cttgctgcta cagatgcaat acttatagca tctgcttctc
     4321 ctttatatgc attggttagc ataacagtat tgactttttt tattgaattt atttcttttg
     4381 caacattata acttgttttt attctgtcat ttcccttaat tcttacaact ttcattttct
     4441 tagattttaa tgaattttcg acagatgtac taatagaata tgttcctcca attatataaa
     4501 cttttgatac attttttaat cttgcagatg tctcagttgg tatagtattt ttttcagtta
     4561 atagtatagg ggcatttaaa gctcctgcta gtccacttgc acttaatcca tctgcaatag
     4621 agttactagt attaattaat atagcagttt tatatgcagt tttatatgca gttttatatg
     4681 cagttttata tgcattttta tctgctattt tacctgctat ttcatactta ttttttcctt
     4741 gtattttttc tacagatgat attgcatcta ctaattgaat atttgcaaat acaaaagaaa
     4801 tacttaaagt caatgttaat attcttttat agaatttcat aataaaattg tccccctcat
     4861 aataaatttt taatatataa atacatattc tt
//
LOCUS       DAELNY010000228         4763 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.231, whole genome shotgun sequence.
ACCESSION   DAELNY010000228 DAELNY010000000
VERSION     DAELNY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.231"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            185..1336
                     /locus_tag="KPE55_003398"
     CDS             185..1336
                     /locus_tag="KPE55_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG0494430.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1382..2557
                     /gene="thiI"
                     /locus_tag="KPE55_003399"
     CDS             1382..2557
                     /gene="thiI"
                     /locus_tag="KPE55_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG0494431.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2713..3516
                     /locus_tag="KPE55_003400"
     CDS             2713..3516
                     /locus_tag="KPE55_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0494432.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3759..4400
                     /locus_tag="KPE55_003401"
     CDS             3759..4400
                     /locus_tag="KPE55_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG0494433.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1915 a          452 c          921 g         1475 t
ORIGIN      
        1 tatagtataa gtatattata ttttactata aaaatttgta ataaagtgaa aaaactagct
       61 tttcatgaaa taatatttta taatttaaga ttgtcttttt aataaagatt aacaaaaaaa
      121 atactataat tagtggtata atagttagag ttgaagattt taaataaagg taggtgtaat
      181 taagttggaa atatacttag acaatagcgc aactacaaaa ccttatcaag aagttataga
      241 taaaatggtt tatgcactta atacggaata tggaaatcct tcttctgtcc atagaaaagg
      301 tgtagaagtt gaaaaagcta taaaagaagt aagacaagat atagctaaat ctctaggagc
      361 gaaagaaaag gagatttatt ttacatcagg tggtacagag tgtaataata ctataataag
      421 aggtattact agtttaaata aaaaaagaaa aaatcacata atatctacta acatagaaca
      481 tccttctgtt ttaaatacat taaaagattt agaagaagat ggttttgaag taacttattt
      541 agaggtcgga aaagatggga aaataaatat agaggattta aaaaatgcga taaagtcaac
      601 tacatgctta gtaagcatga tgcatgttaa caatgaaata ggaactatac agccaatagg
      661 tgaagttgga aagtatttga agggtttaaa agaaaaaatt tatttccatg ttgatgctat
      721 tcagtcatat ggaaaaatta actttagacc atctaaatac aatatagatt ttatgagtgt
      781 aagtgcacat aaatttcatg gtccaaaggg aattggattt atgtatataa aagaaaataa
      841 tcgccttaag cctatgttaa caggtggtgg tcaggaaatt ggaattaggt cgggaacaga
      901 aaatgtacct ggaatatatg gtcttggaga agctgttaga atactaaaca aagatttaga
      961 tgctgttatt tcgaaagtaa ataacttaaa aaatatatta aaaaatgaaa taattgataa
     1021 tatagaagat ataaaaatta attctccaga agatggagta tgtcatatat taaatgtatc
     1081 atttagaggg acaaaaggag aagttttact tcattactta gagcaaaaag gtatttatgt
     1141 atcaactggc tctgcatgtt cttctaaaaa gaaaggtagt tacgtattaa atgctatagg
     1201 tcttacaaat gaagaaataa atggaacaat aagatttagt ttatctgata tgaatacaga
     1261 agaagaaatg ttagaagctg tgaaagtttt gaaagaatca atttgtgact taaggtcaat
     1321 aatgaaaaga aaataaagaa tactgtatag atagtgataa taaattagtt taagaggtga
     1381 aatggtgtat aatatattaa tagtaaagta tggagaaatt ggagtaaaag gaaagaatag
     1441 atatatattt gagaataggc ttataagaaa tataagaaat atgttaaaac caattggaag
     1501 atttaatgta tataaagagt atggaagaat atatgttgat ttagaagact atgattatga
     1561 agaagtaatt gaagaagtta gaaaagtatt tgggatagtg ggtgtgtgcc ctggagtaag
     1621 agctaaaaaa gattatgata cattaaaaga aatagcttta aaaatgttag aagaaaaaat
     1681 agaggctgga tataaaactt ttaaggtaga gtcaagaaga ggagataagt ctttcgggtt
     1741 gacttctcaa gaaatgagta tggatatagg tggatactta ttatcaaagg taggagatag
     1801 gattaatgtt gatgtaagaa atccagaagt taaaataaag tgcgaataca gagaatttca
     1861 tactatggtc tatagtgata ctatacctgg atatggtgga ttgccacttg gaacgaatgg
     1921 tagagctatg tctcttttat caggaggtat agatagccca gtagctacat ggatggttgc
     1981 aaaaagaggt atggaagtag aggcagttca ctttcatagt tatccattta caagtgaaag
     2041 gtcacaggaa aaagttaagg atttagctaa aatactggca aaatactgtg gtagagtaag
     2101 gcttcataaa gttaatatct tagaaataca aaaagcaatt ggcgagaatt gtaatgaaga
     2161 agaagctact attctttcaa gaagatttat gatgaggata gcacagagac tttctgaaaa
     2221 aagacattgt gatgctttaa ttacaggtga aagtattggg caggttgcat ctcaaactat
     2281 acaaggactt acttgtacta atgccgttgt agacttacca gtgtttagac cacttatagc
     2341 aatggataaa tcagatatag ttgatatagc caaaaaaata gggacttttg aaacatctat
     2401 attgcctgaa gaagattgtt gtagtgtatt ttctcctaga aagcccgtca ctaaacctag
     2461 attagagaaa atagaaaaat ctgaaacagc acttgacatt gaaaaattag tacaggatgc
     2521 tattgacaaa atagaggtag aggatattga gttttagcat acaagttatt taattactaa
     2581 attgaatatt gagtatatta gtagttctgt aagtatatta gtagttctgt tgaaaggatt
     2641 tatgtagaca atttattaat atgtagtatt taaaaataag tactataatt ttatttaatg
     2701 ggaatgatta ttatgaataa aaagggtttt acactaattg aattgttagt agttatatct
     2761 ataataggca ttttagttat agtagctgtt ccagcattgt ttagaaatat agaaaaaagt
     2821 aaagcagtta catgtctttc taatagagaa aatataaaga ctcaaattgt tattgcaatg
     2881 gctgaggaat caagtaaagg caagaatgaa gtcatgaaag aggtattaga aaacaaagat
     2941 ggtaagtact ttgaaacaga accaaagtgt aagtcaggtg gaatatattc agcaacgttt
     3001 gatgatggtt atgatggaat aactggaata gaaagcattg caaaagtgta tgttacttgt
     3061 acaaaacatc cagacggtgt tgaaatggct agggatatac atcaaagtat gaaagatttg
     3121 attgcatcat ttgcacaaga cccttctata ataccaggag cttcaaaggg taatgatgat
     3181 tttagaaaat atttattaga caataaatat aaaaatgggt ggcctacaat tccagatgaa
     3241 tttaaggcaa aatatggatt aagtaaggat acactatata tacaaccata tgcatatagt
     3301 cctactaaat ctgatgctac tgtagttgta tttgcaaata ataagactgg gggtaattgg
     3361 tatacttccc tagtttacga ttatgatgaa ggtagatggt ataaaggtaa aaatggtatt
     3421 tctgttgcag gtaggtcatg ggatgttgac acagatagtg ttaagtctgt aaaaacagag
     3481 attcattcta aagagggatg gggtccttta aattaatata tcatgtttta ttaatctggt
     3541 attgattaaa ctatagaaaa gagcagtttc tatagatgac gaataccaga ttttcattta
     3601 tatatataat tgagatatat taaaagtgtt tatattttta agtcttcaca tacaagctaa
     3661 aaataattat aataatattg taaagaaatt taattataag attaattttc tgaagattta
     3721 gtatttgtac taagaaatac ataaaaaggg gaatagatat gaataataat tttcaagaca
     3781 accaaacaat aaatttaata caaagtcgta gaagtatacg aaaatttacg acagaacaaa
     3841 ttagtgatga gcaagtaaac acattattac actgtgcatt tgcagcacct tcaggatgta
     3901 ataaacagcc ttggcacata actgttgtgc aagaccagaa acttttaaaa gagattagtg
     3961 atgatacttt atctaggatt catgaggtga gtaatgttga gataaataaa aactttaagt
     4021 tattttatgg tgctccaacc gttttattta tttcttatga tgaaagtagt tcatgggctc
     4081 catatgatat aggaatactt acagggaata taacaacagc tgcacaagca ctagggcttg
     4141 gaagttgtat aataggaatg gttagaggtt tatttacacc tgttgaacaa ggggatattg
     4201 aaggtcttgt gagtgtattg gataaagaag atgtaaaaga atcagagtca ataaagatga
     4261 agtttgatac aaataaaaaa tacagagaac tacttgatat accagaaggg tattctgtac
     4321 catttggtat tgctgtaggt attccagatg gaaatttacc aaatgctaga gaggtagttt
     4381 acaaagtttc cagagtttag gataaagtat tgcaagaacc aatcagttac ttgaagaatt
     4441 aaaccagtaa tatacaaata aattgatgga agaggaagct atttaaaaag tagtttagat
     4501 ttttcgttgt aaaataatta aataaagaat aaggtgataa gtattttatt tattatctta
     4561 ttctttttta tttttgtttt gtagaatgat aaaaataagg attaagtgta ataaaataga
     4621 aaacaagtat gttaatttta agtatatgat ttaaaattta tccaagttaa aaatttaaga
     4681 tatcttttca gtataatgga aaaaatgtca aaatattgaa atagggaaaa aatagtgata
     4741 taataagaaa aaatagagaa aaa
//
LOCUS       DAELNY010000229         4747 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.232, whole genome shotgun sequence.
ACCESSION   DAELNY010000229 DAELNY010000000
VERSION     DAELNY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.232"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            34..501
                     /locus_tag="KPE55_003402"
     CDS             34..501
                     /locus_tag="KPE55_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBG0494434.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
     gene            1632..2294
                     /locus_tag="KPE55_003403"
     CDS             1632..2294
                     /locus_tag="KPE55_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494435.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            2303..>4747
                     /locus_tag="KPE55_003404"
     CDS             2303..>4747
                     /locus_tag="KPE55_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494436.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIVVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMIT"
BASE COUNT         2057 a          399 c          740 g         1551 t
ORIGIN      
        1 ataaataaag tatgtgtata ttaaggaggt aatatgaagt ttttaaatgt tttagtagta
       61 gttgaagata ttgagaaatc aaaaaagttt tattatgatg ttttggggtt aaaggtaatc
      121 tgtgattttg gagaaaatgt tgtattagaa ggtaatatat cattacaaga aaaaaagctt
      181 tggttagaat ttattaataa gagtgacagt gaagttaagt tcaatggaaa tgatgctgag
      241 ttatattttg aggaagataa ttttgatact tttgttgaaa gattgagtac aatgaaagat
      301 atagattatg ttcatctagc tatagagcat aggtggggtc aaagagcaat tagattttat
      361 gatttagatg ggcatattat tgaggttgga gaaactatgt catctgtgtg tagacgtttt
      421 ttagatagtg gtctttcaat agatgaggta gctaaaagaa tggatgtaac tgtcgaatat
      481 attgaatcag ttttagaata gatggattat attaaagtaa aattgaggtg aaggctgtaa
      541 gtaaacttgt gtgaaaacaa atttgcttac agtttttatt tttctaaaat aaagaatata
      601 atacttgttt agctttattt gagtttttaa aaatattatg atatttctta tatatttata
      661 ctaaagttta aatttagaaa aaatatataa aagatagtta tatttactat tatcttgtat
      721 aattatatat actattttat ataactatgc taaaatttaa ttgaataaat taaaaatata
      781 atgaaagttt agttttaatt catttattta ataaacattt caagtaaaat aatgtttaaa
      841 tcagaaatat aaataaatat tattataatc gttattaaag accaaaaatc ccaccttaga
      901 agtttgtatc atatgaaacc tttatagatt atgaaagcaa taatatagtg tatgatatat
      961 aaaataagtt ataaaagtat ttttttagat attcaaaaag acattgtaag ttatattaat
     1021 aatatgggta tcaacgaatt ttggatataa tgagtttttc aacttaaagt tatgtaataa
     1081 tgaatgtaaa gaatatttaa tgaaatcatt ttgttggtta tgtctagctg aatacaataa
     1141 tcttttagat aaagatttta tatttgaaga taggtgtgaa aaaattaaga agatattaac
     1201 atcagcagtt tagaggcaaa gcatgtaaag taaagtctgg gtaactaaaa gtagtaattt
     1261 agattgtaac tattgttata aatatgaaaa tgaggaaaca ggaaatataa gttaggatgt
     1321 agaaaatagg ttactaaata aaaagcttaa ttatataatc aaagaaatag gtatatactg
     1381 tacaatttca tggtgaagaa cttttcctaa ataaaaaggt tggtaaaaga gatataagaa
     1441 ataaaattca attataagat atggtatgat aactaaaggc actatatgga gtgaataggc
     1501 ggaagaattt tttgaaaatt ataaaaatag tttctgttgc tacatatcaa taagtataga
     1561 tagagattgc aaaatccata gcaaaaatcg aatttataag tgtaaataac atgtaagaca
     1621 aggagtaaat tatgagtatt ttagagataa aaaacctaaa taaaatatat ggtaaatcgg
     1681 aaacagaggt gaaagcgtta agtcaaataa atctaaaaat aaatagtgga gaatttgttg
     1741 tgatagtagg taaaagtgga agtggtaaaa gtactttact acacataatg gcaggacttg
     1801 aaactccaac aaatggtgat gtgattattg ataatataaa tatatcatct cttgatgaaa
     1861 agaaaagatc tgcattacgt aaagaaaagt tgggattaat atttcaatca tataatttga
     1921 ttccagtttt aactgttgaa gaaaatataa aactaccaac tataaatata aaaaataaag
     1981 atgaagctta tattaatgaa ttgatggagt tactaggaat aaaagataga agaatgtatt
     2041 tgccaaatca attatcaggt gggcaacaac aaagagtagc aatagcaagg gctcttgtaa
     2101 ataaaccatc aattgtattt gctgatgaac ctactggaaa tttagacaca aagacagagt
     2161 cagatgtatt aagtttatta aaagaatctc aaaaaaaata taatcagaca ataataatga
     2221 taacacataa catagatata acaaaatatg ctgatagagt tatagaaata gaagatggat
     2281 ttataagaga gtaggtagat ttatgaaact taattatatt gtaaaaaaat atataatggg
     2341 aaataagaaa aatacattat taatagttgt aagtattgta atttcaacag cgttgttttt
     2401 aattatgaat ataataagtg aagatgcgac aaacttaatg ataaatcaag ctaaaaatga
     2461 atttacatta aaacatgcac aatatatcaa tccaaaagac gaagaaataa aatatataga
     2521 aaataacaca agtatagata aagttggaaa atcaatgctt ttaggaatta gtgatattgg
     2581 taaaggtcaa acactacaaa ttctatctca ggataaagtt gctgaagaac taaatgatat
     2641 ttacacacta gaaaaaggta aattaccagt agctgaaaat gaaatcgcta tagattcatg
     2701 gtatattaaa caaaaaaaga taaaaaaccc tataggaaaa aggataacat tagattatag
     2761 aagacaaggg atagatcaag aagatttata tactggggaa aaagaattta aaataactgg
     2821 tatcttaaat agtaatccta ttttaaaagc tcaaggaaca tcaattggcc ttataagtaa
     2881 cgattgtgct atcaagaata ttcctactaa aaataaatat gatcaagtta tgtttacttt
     2941 taaaaaggaa aaaaatatac agagacaatc acaaaaactt attaaaaatg gaaatttgaa
     3001 tgagaataac atttatttta ataatgaatt attaatagct atatctgata gtatgaattt
     3061 aaaaatacct tatataatag ttaatatagt actagcacta gcaactatat tattaattta
     3121 taatattttt tatatattag tatcaaatag aataaaggat tttgggatat ttagggcttt
     3181 agggtttaat ccaaatgata tttttaaaat aatgatttta gaagtatcta tatactctat
     3241 tataagtatt tctataggtc taattttagg tgggataata gctagtttat caagggagta
     3301 tgttatagga gtaatataca atgtcaacta tataaattct ataaaaaacc agaactatat
     3361 aaacacatac ataatttcta tattattaag tttaggaact ataataatat cagtttcaaa
     3421 acctcttatg ttatcagtta aaactgatcc aatgatttgt ataagaagaa ataatgaaaa
     3481 agtggatata aaacaaaatt cctttataaa taaatttatg ataaaacttt tcaaagacta
     3541 tggtaacata gcatcaaaaa atatacaaag aaataagaaa cgtacaaatt tatctatagc
     3601 atcaatggta attatatttt tcttaatggc aacaatatac acaaagtcta ctagtaactt
     3661 tttaagtgat ggtgtattaa aatattggat acaaggagat tatttattac ataatattga
     3721 tataagtact atacgttcaa ataataaagg ttatgataaa aatgtactac aagagataaa
     3781 ggcaattgat ggtgtaacaa aagtaaactc atataggcat aagtggttta atataaaaat
     3841 tgatgataaa agtataaata agaactctga ttattggaaa aaaaataaag ataatattga
     3901 agctagatca gaagtaaaag atggaatcaa ggtatataat aattcttttg aatttttagg
     3961 aatagaagat acagatatac tagaggattt cttgataaat ggaaaggaaa atatagataa
     4021 atttagtaaa caaccatatc tgtatataac aaagaaatcc agtgattcgc ttaatataaa
     4081 aaaaggagat agaataaaag tatactttga tattatagat tcaaaaacaa ataattttac
     4141 caaaacaata tctcaagaat ttatagtatg tggaattata actacattac cagtaacctc
     4201 acaagtggga gctgaatttg gcggagtaat atcaacaaat caatttaata agtttacagg
     4261 aatatcatct tatgaaagat ttgacatatg gacgagtaaa ctagccaatg ataaatatgt
     4321 tgaaagtgag ttaaacaaaa taacagaaaa aacaaataaa ggaattttaa ttccatacaa
     4381 agctgaaaca gctgaatatg aaaaaattga taatcaaaaa acactaatta tggtattagt
     4441 aactggaatt atagttatat tatctttatt taactgttgc aatactatag ttactagtat
     4501 taatagcaga agtagggaat ttgcactatt tagaggaata ggaatatcaa aacatgaaat
     4561 aaataaaatt gttaaattgg aaagttatat atatattata gtaagctttt gtatatctat
     4621 aatacctatt ttaattgtaa gaagtataat aataaaacct tttgaaagta tcaatttaat
     4681 aaattggaaa tttatagggg ctattatcct aatagtattt attttagtat ctataatcat
     4741 gataact
//
LOCUS       DAELNY010000230         4682 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.233, whole genome shotgun sequence.
ACCESSION   DAELNY010000230 DAELNY010000000
VERSION     DAELNY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.233"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      <1..62
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            245..817
                     /locus_tag="KPE55_003405"
     CDS             245..817
                     /locus_tag="KPE55_003405"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG0494437.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            881..1531
                     /gene="fsa"
                     /locus_tag="KPE55_003406"
     CDS             881..1531
                     /gene="fsa"
                     /locus_tag="KPE55_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0494438.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            1683..4607
                     /locus_tag="KPE55_003407"
     CDS             1683..4607
                     /locus_tag="KPE55_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0494439.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         1894 a          431 c          920 g         1437 t
ORIGIN      
        1 ggttcaaatg tttctactaa gctaccatta atagcttgac tacaggagtg aagaagatat
       61 agtttatact tattttaaat tatatattaa gttaattact ttgaatttta agctcctgtc
      121 tatgatatag acaggagctt ttttagtatt ttaatataaa atacttacaa gaatagatag
      181 caaaattaga acaaatataa tttttttatt aatataattt tagaaagggc taggtggaag
      241 ttcaatgaag gctttacaag aaaagatatt gagagaaggt agtgtttcag gaaatgatat
      301 actaaaagtt gatagttttt taaaccatca aattgatgta gcttttttaa atgaaatagg
      361 aagagaattt aaagagagat ttaaaggaga aaaggtggat aagatattta caatagaggc
      421 ttcaggaata gctatagcat caattgtttc acagtatttt gataatgcac ctgtagtatt
      481 tgcaaaaaaa tcagaatcta aaaatttgga taaagatgta tatgaaacta atgtatattc
      541 atttactaag gcaagagagt attcagttaa agtttctaaa aagtacataa acaaaggcga
      601 aaatatattg atagtggatg attttttggc aaatggaaga gctgctcttg gacttaaaga
      661 tttaattgag caagcagaag ctaacttagt tggagtagga attgtcattg aaaaaggatt
      721 tcaagctgga ggagcacttt taaaggctaa tgatgtaaga ttagagtctt tggcagtagt
      781 tgaatctata gataatggaa ctgtaaaatt taggtaattt atgatattat atatttgtgt
      841 ttagcacata ttttaactat gtaattatgg aggatttttt atgagatttt ttatagacac
      901 agccaatatt gaagaaataa aagaagcaaa tgatttaggt gtaatctgtg gtgtaactac
      961 taatccgtct ctaatagcta aagaaggtag agattttata gaggtagtta aagaaataag
     1021 tgaaatagta gatggaccaa taagtgcaga agtaataagt ttagaacata aaggtatgat
     1081 agaggaagca gaaaaattat ctaagataca taaaaatata gttataaaga tacctatgac
     1141 agcagaaggt ctaaaagcgg tgaaagtact atcaagtaaa ggtataaaga ctaatgtaac
     1201 gcttatattc tctgctggac aagctctttt agctgctaga gctggagcta catatgtaag
     1261 tccttttgtt ggtagattgg atgatatatc acaaaatggt cttgatttaa ttgaagaaat
     1321 cgttgatata tttagtgtaa atagtattga ggctcaaata atagttgcta gtgttagaaa
     1381 tccaatacat gttttacaag ctgctagaat gggagcagat atagcaacag ttcctcttaa
     1441 agttataaac caaatgatta agcatccttt gacagacaaa ggaatagata gctttatgaa
     1501 ggattgggaa ggtgcttctt taaaattata attataaact acgctttata agtatattaa
     1561 agaggttatt atggaaattc taagtaataa ggctaggcta ttttgcctag tcttattttg
     1621 gtgtataata tattgaaaca tattattgaa atagattaat tttttaagtg aggaggcgaa
     1681 aaatggataa aatattaaga gagatacaaa gagaagataa aaaaaatcca tatactgacc
     1741 aagaacttgc tgaaatgtta aatgtagcta ggtctgaagt gatatcagtt aggaaaaaaa
     1801 ataatatttt agattcgaga gaaagaagaa aaaaaatatt aataaaagat atatcaaaaa
     1861 ttgtaagtga aaatcctcaa atgtctgaaa gaaaaataac tgaaatatta atagaaaatg
     1921 gatataatat atcgagaagt gcagtttcaa aattattaaa agaggaaaat ttaaatcagt
     1981 caaagattgg gattaaggat aaagacccag catcatcagg aaagtatgtg aaggtgagta
     2041 aagttgtaga agaagatgga tttgaagaat tgataggtat aaagggaagt ttgaaagaaa
     2101 aggtaaattt agctaagtca gcaataatgt atcctcctaa tggccttcat actataatat
     2161 atggagaaac aggagtaggt aaaagtgaat tagctacatg tatgtataaa tatgctataa
     2221 aaaacaacat taaagaagag aacagcccat ttatagtgtt taactgcgca gactatgcag
     2281 aaaatcctaa tctattgata gctcaactct ttggggtagt aaaaggtgca tatacaggag
     2341 cagatgcaaa tagagaaggt cttgtagaac aagctaataa tggaatattg tttttagatg
     2401 aaattcatag attgcctgct gctgggcaag aaatcttatt ttcattaata gatagaggag
     2461 aatttagaag gttaggagaa agtagttcta atagaaaggc aaatgttctc ataataagtg
     2521 ctacaacaga aaatccagat tctaatttac ttcaaacatt tagaagacgt ataccaatgg
     2581 taataaattt acctaatatg agtgaaagac caaaatctga aagatatgaa ataataataa
     2641 agttttttga aagagaagca aagcgagtaa acaagaattt tataataact aaagaagtta
     2701 tgtgtgcact tatgaattat aagtgtactg gaaatatagg tcagttaaag agtgatatac
     2761 aagtaacttg tgctagagct tttagtaaat cgatattttc tagtgataaa gtaattatag
     2821 atttagatag tttaagggat tatataaaaa gtggatatta tgattgtaat tgtaaaaatg
     2881 aaaagtattc tgagttttca gttgaggata tatatataga tattagtgaa ttggtgagtg
     2941 gaaaaaatag aattttatct gataatgaga taagcgagat atataattat gcagaaaaag
     3001 agcttaaggt tttggaaagt aagtgctttt cagaagaaga gttaaaaaat gcatttatag
     3061 aaaagttaga taagaagttt aatgctatta aaaacaataa aaatttatct gatagaagaa
     3121 gtcgaataga ctggggagga caattaaaag agtctacact ggagataatg gataaggtaa
     3181 ttgtatttat taaggagcag tttaagcatg taaatcaagg gctgtacttg gctctagcaa
     3241 ttcatattga gcatgctata ggtaggataa aagatggaaa aactataata aatccgagtt
     3301 tagataaaat aaaagcatca atgccaatgg aatatgagtt gtcaagatat ataatgggta
     3361 tagtagaaga tttgacacaa gtaacatttc cagaagatga gcttggatat ttggcttatt
     3421 atataaataa attttgttat aatgaggaaa gtataaaaga taaagttaaa gtagtaatag
     3481 ttacgcatgg taaagttgga attgaaatgt ctaaagttgt aaatcatatt ttaggtatag
     3541 aatgtacact aggtatagaa atagcactta cagattcacc atcagagggg atagagcatg
     3601 tattagaaga gcttcaaaag atagaagcta gaaaaggtat tctagttcta attgatatgg
     3661 gttctttggt aattcttggt gatgaggttg aaaaaaggct tggaattaga tgtaaaactg
     3721 taaatagagt agatacatta ttggcaatgg aagcaggaaa acttgcaacc attgaaggga
     3781 aaagtttaga tggtataata gctgatttaa agaaaaataa gaattatgct atgataaata
     3841 caaataaatt tagttacaga aaaaacgagt atggaaagaa aaatgtaatc attactttat
     3901 gtttgagtgg tgtaggtaca gctttaaatt taaaagagca tatagaaaaa caaattaagg
     3961 aatatgacac tttaattgaa gtaaagccag ttgctttttt aaataataat ttagaagaag
     4021 agttaaatga tattgaagct gaatataata ttattgctat ctgtggaact attgatatag
     4081 attataaaaa cgtgcctttt atttcttata atgaggtact tggtaaaaat ggtattaata
     4141 aagtattaga gcatttaaat agtaaacaac cagctttaaa tattgaaaac cagttgaata
     4201 atttaataca tgaagatttg attttataca attttgaagg aatttcaaag gagtatataa
     4261 ttgatactct tgtttcaaaa cttgaagaag gtggatatgt cgactcaaag tatatattaa
     4321 gtgtatataa aagagaagca atgggaagtg ttgtaatggc ctctaaggta gccgtgccac
     4381 atggacttcc agaaaacgtt ataaagcctg caatagcaat tgcgaggttg aataaaccta
     4441 tagtatggga caataaattt atggtggatt tggtggtact actggcactt aaagaaaata
     4501 ataagaaaga aatacgaagc ttattttcaa agattaatga ccagcatact ttagaagttt
     4561 tattaaatac tgaagataaa aatgaaataa aagaactttt aagctaattg ttagtaccga
     4621 gaagaaagac aaaaaaattt gctttagtag tataaaattt aaaactactc aattgctaag
     4681 ta
//
LOCUS       DAELNY010000231         4641 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.234, whole genome shotgun sequence.
ACCESSION   DAELNY010000231 DAELNY010000000
VERSION     DAELNY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.234"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..861
                     /locus_tag="KPE55_003408"
     CDS             <1..861
                     /locus_tag="KPE55_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0494440.1"
                     /translation="ENTVASYFMDIKKYMEYINQKSIKLVDIVENDIISYLIKLEKDN
                     VSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIKKENINILTEEEIERLLNF
                     PKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDIDYIYCNSCCKNQRVIPLC
                     DITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGLWKVIKKYSNLANIDKNIN
                     PTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNHIDKNVRREIKEKHPRNDV
                     DVELQKAEAK"
     gene            903..2075
                     /locus_tag="KPE55_003409"
     CDS             903..2075
                     /locus_tag="KPE55_003409"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG0494441.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            2122..2934
                     /locus_tag="KPE55_003410"
     CDS             2122..2934
                     /locus_tag="KPE55_003410"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG0494442.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            3138..4463
                     /locus_tag="KPE55_003411"
     CDS             3138..4463
                     /locus_tag="KPE55_003411"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG0494443.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT         1848 a          477 c          892 g         1424 t
ORIGIN      
        1 gagaatacag tggcatcata ctttatggat ataaaaaaat atatggaata tataaatcaa
       61 aaaagcatta aactagtaga tatagtagaa aatgatatta taagttactt aataaagttg
      121 gaaaaagata atgtatcaat agctacaata gcgaggatga tatcctctat aaaatctttt
      181 catgattatt tatttttaaa tcatatatgt acaaataatc ctgcaaagga tataaaaaaa
      241 cctaaaataa aaaaagaaaa tataaatata cttacagaag aagaaattga aagactatta
      301 aattttccaa agttaacaac acctaaattg attagggaca aggctatttt tgaagtatta
      361 tatggtactg gtataaaagt atctgaatta gtagagatga atatagagga tattgaccta
      421 gatattgatt atatatattg taattcatgt tgtaaaaatc aaagagtgat accactttgt
      481 gatattacaa aactttactt agaaaagtat ctaaaagaag caagacctaa aatggctgta
      541 gaaggtgaaa aatcattatt cgtaagttca ttaggtcaaa ggtttacacg acaaggtctt
      601 tggaaagtca taaaaaaata ttctaatcta gccaatatag ataaaaatat aaatcctaca
      661 atgcttagac attcatttgc tattcatttg ttaaatgaag gtgctaacat tgctgttgta
      721 agtaaaattt taggaaatgt taatttatct agtttgcaag tatatttaaa tcatatagat
      781 aaaaacgtga gaagagaaat aaaggagaaa catcctagaa atgatgttga tgtagaatta
      841 caaaaagcag aagctaaata gactatatag caaattttat aatttagaag gagcatattt
      901 atatgagcag agtaatttgg attgtaattg atagtgttgg gataggagca ttgcctgatg
      961 ctgaaaaatt tggtgatagt aaggatgtaa gtacccttgg aaatatattt aaggaatatc
     1021 cagatattca gattcctaat atgagaaatt taggtatagg aaacatagat ggtatagatt
     1081 tttttgaaag tataaaagaa ccaataggtt gttttggaaa gtgtaaggaa atgtctcagg
     1141 gaaaagatac tactacaggt cattgggaaa tgactggtat aatagttgac aaaccattta
     1201 agacatttga acatggattc tctaaggaga taatagaaga gtttgagaaa aaaactggaa
     1261 gaaaagtagt aggaaataaa ccagcttcag gaactgtaat aatagatgag tatggagaac
     1321 atcaaataaa aactggagat gtaatagtat atacatcagc tgatagtgta tttcagattg
     1381 ctgctaatga agaagtaatt cccctagaag aactctataa tatgtgcaaa atcgctaggg
     1441 aaataatgat gggggataat gcagtagcta gagtaattgc tagaccattc ataggtaaga
     1501 aaaaaggtga gtttgtgagg acttcaaata gaagagatta ttcactagac ccatttgaac
     1561 caacagttct tgataatata aaagaatcag gacttgatgt tttagcagtt ggaaaaatag
     1621 aagatatttt taatggaaaa ggaattacag atgctatcca cactaaaagc aatatggatg
     1681 gagtggatga aactttaaat tatatgaaac aagataataa aggtctaata tattcaaatc
     1741 tcgttgattt tgattctaaa tatggtcata ggagagaccc agaaggatat aagaaagctc
     1801 ttgaagagtt tgatagtaga cttcctgaaa taatggctaa tatgagagaa gatgatattt
     1861 taataatcaa tgcagaccat ggaaatgacc caacatataa aggtacagac catacaagag
     1921 aatatatacc tgttatgatt tatggcaata agattaaaaa aggttttaat ttaggagtaa
     1981 aagatacttt tgctgatatt ggagcaactg ttgcagatat actaaatgta aagttgccta
     2041 aacatgggtc aagctttaaa gaagatttat tttaaactat aaaatgataa aaacttagct
     2101 taataggatg gagagattgg aatgtttgaa aagatagcac aaagtagtaa atttataaat
     2161 tctaaaagtg atataaaacc taagattggt ttgatattag gttctggact aggtgattta
     2221 gcaaatgata tagaagaccc tgtcgcaatt aaatatagtg aaattccaaa cttccctgta
     2281 tcaacagtag caggacatgc tggacaactt gtaattggaa aacttgaagg aaaagaagtt
     2341 attgctatgc aaggtagatt tcattactat gaaggatatt ctcagaaaga agctactttt
     2401 ccagtaaggg ttatgaaaga attaggagtc gagattctta tagtaactaa cgctgctgga
     2461 ggcgtaaata aagaatttaa agcaggagat ttgatgctta taagagacca tataaacttt
     2521 agtggaagta atccattagt aggaaaaaat gatgacagat ttggtgctag atttccagat
     2581 atgtcagatg catattcttc taaatatgta gatgtagtta aaaactgcgc taaagaatgc
     2641 aatatagatg tcaaagaagg cgtttatatg ttttttagtg gtccaaacta tgaaacacca
     2701 gcagaagtga gaatggcaca aattttggga gctgatgctg taggtatgtc tactgttcca
     2761 gaagtcatag tagcttctca ctctaatata ggagttattg gtatatcttg tataacaaat
     2821 atggctgctg gcatcttaaa tcaaccacta agtcatgagg aagtaataga aactactcaa
     2881 aaagttaaat cagaatttat gaatttagtt aaaacaacgg ttaaaaattt ataagttaaa
     2941 gcttattata gattttttga ggaagtttat aataaaatag ttaatgttta atttgaaaag
     3001 aattaataaa aagctgtctt gatatagaaa gttatttcta ataaaggaca gtttttttat
     3061 aatactacaa catttgaaag tgttttaaag tttaatattt atttaaatta ttataggtta
     3121 gaatggagat ttcaattatg agaatgtatg atattataaa aaaaaagaga gataacctag
     3181 agcttagtaa aaaagagata aactacttta tagaaaacta ttcaaaggga gatatacctg
     3241 actatcaagc ttctgcacta ttaatgtcga tttatttaaa taagatgaat aaacaggaaa
     3301 ctgtacatct tacagaagca atgatgaact ctggagacat gataaaccta tctgagatag
     3361 atggaataaa ggtagataag catagtactg gtggtgttgg agataagact accatagctc
     3421 ttataccttt ggttgcttca tgtggagcac cagttgcaaa gatgtcaggt agaggtcttg
     3481 gtcatactgg tggtactatt gataaattgg agtctatacc aggtttttcg acagaaatgg
     3541 agataagtaa atttattgat tctgtaaata agtctaaaat agcagtttgt ggtcaaagtg
     3601 caaaggtagc aattgcagat aagaaaatat atgctcttag agatgttaca gcaactgttg
     3661 ataatatatc attgatatca tcaagtgtaa tgtctaagaa gttagcttca ggagctgatg
     3721 caattgtgct agatgtaaaa actggtgatg gagcatttat gaagacatta gaagagtctt
     3781 ttgagttagc taaatctatg gttgatatag gtactggtat gaatagagat acaataggga
     3841 ttgtaacaga tatggatgaa cctttaggtt ttgcagtggg aaactcttta gaagttatag
     3901 aagcaattga gacattaaaa ggaaatggac ctaaagacct tgttgagcta tgcgaagtat
     3961 taggagcata tatgttggtt ttatcaaagg tagcacatga ttttgaagat ggtgttgaaa
     4021 aaataagaga atcaataaaa tctggtactg ctatacaaaa actcaaagag tttatagaga
     4081 atcaaggtgg taataaagat gtagtagatg attattcttt attcaaaaaa tcaaagtata
     4141 aatatccagt taaatcaact aaatcaggtt atataagcaa aattaaagct gaagatgttg
     4201 gtgtatcggc tatgattctt ggagcaggta gagaaactaa agatgatatt ttagatttat
     4261 cagcagggat aattttagaa aagaaagtag gagattatgt aaatgaaggt gaagttttag
     4321 cttacatgta ttataatgat gaacaaaagc tttcattggc aaagggaaga tttattgatt
     4381 cctactctat tgtagattca aaaatagaaa aaaataaatt aatatatggt attgtaacta
     4441 aagaagagat taaaaagttt tagaaaattt tagaaaagtg attataaaag gcctaacatg
     4501 tcaaaaataa tgttaggttt tttatttttt gatagactat aaattatacc aaaaatatat
     4561 attttaaaac attttttaca atataaataa gtttattcta caagatgatt gaaaattaaa
     4621 gtatctagtt ttataataga a
//
LOCUS       DAELNY010000232         4639 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.235, whole genome shotgun sequence.
ACCESSION   DAELNY010000232 DAELNY010000000
VERSION     DAELNY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.235"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            322..699
                     /locus_tag="KPE55_003412"
     CDS             322..699
                     /locus_tag="KPE55_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0494444.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            692..1840
                     /locus_tag="KPE55_003413"
     CDS             692..1840
                     /locus_tag="KPE55_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG0494445.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1857..2459
                     /locus_tag="KPE55_003414"
     CDS             1857..2459
                     /locus_tag="KPE55_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0494446.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2552..3574)
                     /locus_tag="KPE55_003415"
     CDS             complement(2552..3574)
                     /locus_tag="KPE55_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0494447.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3903..4085)
                     /locus_tag="KPE55_003416"
     CDS             complement(3903..4085)
                     /locus_tag="KPE55_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494448.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4398..>4639
                     /locus_tag="KPE55_003417"
     CDS             4398..>4639
                     /locus_tag="KPE55_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 family protein"
                     /protein_id="HBG0494449.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYH"
BASE COUNT         1825 a          582 c          649 g         1583 t
ORIGIN      
        1 cataattaga aggttattaa aatttattag atattactct agtttatttt aaaaaaaagt
       61 attataatat tccttgtgtg aaaatatagg aatttttatt aaatagtttt aatgtgtaaa
      121 taacattcta cactattgta attaaatatg taaaaagtaa aatatttaaa tagatatatt
      181 ttaaaatcta acttttaata ttgtaaaaaa attagaaatt taacaaaaag tacaaaaatt
      241 cacatttttt tcataaagtg tttgactaaa aaattcacat gttgtagaat taaggtagca
      301 ggagttttga gggaggtgta aataatgata agaaaaatac cacaagcaga actaaaagta
      361 atgaaattca tttggaaagt agatgttaca gtgacatcaa aagatgttat tgaagctatg
      421 gaacaaaaat atggctggaa acaaaccacc acactgacac ttttatctag gctggtaaaa
      481 agaggattct tagatgctca aaaaatagac agatatacac attacacaat aagagtaaaa
      541 cacaaagatt atttaagttt tgagacaaag gatttcctaa gtaatataca tgatgactct
      601 ttaagaagct taatttcagc attacatgat gatgaaaata taggtaaaga taagctagat
      661 tttctagaaa gttattttga taatttaaag gatgaataat tatatgctat atgtttttga
      721 aaatcttata aagacaacta ttgtatgtag tcttggtatt tgtctattat tatttttgaa
      781 aagatattta tttaagaagt ttagtaagag attcaattat tatatttggt tgataatagt
      841 ctttagaatg ttactttttc tatttaatta tacaatagtg tatgaagtaa aagaacccaa
      901 agaaaatgca ttaggaaata atattacaca aattgatatt tctacagata ataatcttat
      961 gctgtatgta gcttatttat ggttatttgt aactatagtc attgcagttt atacatttat
     1021 aaagtataca agatttaaaa atctagttgt tgatgtatct tatgatattg aagataatga
     1081 tataaactgt atctacaaaa acttgttaaa agaattaaat ataaaaaaga aaattgaatt
     1141 aagaggttct gatgaattga taagtccagc aggtatggga ttatttaaat cttacatatt
     1201 cttaccagac tatccttata gtaaagatga actaacttgg attttgaaac atgaattaat
     1261 gcattttaaa aataaagata ttttaataaa gtttttagtt ttgtctgtta gaataatata
     1321 ttggtttaat ccacttgtat atgtaatgag caataaagta aatctagatt gtgaattatg
     1381 ttgtgatgaa agtgttttgg tagaatgttc tttaaaagat aaaaaagaat atgcactagc
     1441 acttataaag tcaattaagt ttagtaaaaa ttataatagt aagattttga caaccgagtt
     1501 tgataagaca aatttggaaa agagacttga gagtattgtt aaaaagaaag gcaaaagtgg
     1561 aatactaatt gctgttttag tatttatgac ttctataaca tcttttgttg aagttgatgc
     1621 tcaagttaga attgatgagg gtaataatgg aatgaataac aatattaatg aatttttatt
     1681 gaataagacg actgataaaa ttataaatat ttattataaa gatgcaccaa atgaaattag
     1741 gaaattttat gaagataaat gcgaattaga aggtaaaata cctagagact atgacataat
     1801 agaaatggat atgaaagatt ataatcaatt aataaaataa ttataaggag gattttatgt
     1861 taacaaaatt aagaaaaata gtagctatta tgtgttgctt attgcttact tttacaagtg
     1921 tggcatttgc tgacacaagt aaagagaaag gaggaagtaa agagactgtt ccagagaaaa
     1981 acctagattc ttttactctt tctgggtata tatatacata tgaaaatgcg ccacaagaaa
     2041 taagagatga acataaacaa aattgtgaag agataaatat agaccctaag ccagatgatg
     2101 agatatttgt tccagaatca gctaatctaa tgaatgagga caatagtaaa ggtgtttatg
     2161 caagttatac tgtttcttat aatataggtg ctaaaactat aacaattatg agtaatgagt
     2221 atttgactat aagtacaaca aaagttatac gaaagggtaa tagcggaaaa gaagtaaaag
     2281 ctgcacaaat catgttaact ttacttggtt ataatgtagg tatagatagt tcttttggaa
     2341 gtaagacata taatgcagtt gtaagtttcc aaaaaaaata cggtttgtct gcagatggaa
     2401 taattggtcc agctacttgg gataaattgg gtagactaac tgaccctact ctttcatagg
     2461 attaaaatct aaaaatatat taaatagtgt ctcaatatga tttttcaatt taaatgagac
     2521 actatttaaa tataaatgtt ataaaactat tttacaataa attccatata actctaccat
     2581 ctatatcctt aacattttga caaccagtca aaatcatagt ctcacataac tcattcctaa
     2641 ccttctctat aaaaaactcc acaccatcaa gaccaccgcc aaaagaagca gtaacaaaag
     2701 gtcttcccat caaaacagca tcagcaccca atccaagcat cttaactaca tcaacaccag
     2761 tcctaactcc accatcaaca agtattgtta tctttccctt tacagactta gctatatctg
     2821 gaagtacctc acaagtacca ggagtataat caagtactct tccaccatga tttgaaacaa
     2881 taatggcact agcaccagac tctacagcca tcaaagcatc atctacagtc ataattcctt
     2941 ttataataaa aggtaatcta gtggattcaa ccaattctct aatctcatca attgttttag
     3001 gagaaaaagg attttcttga aattgattat taataagtcc acaagcatca aggtctacac
     3061 caactgcaaa agcacctgct tcttcagaca atctaatttt atctattatc tttgaattat
     3121 tccaaggctt tataaataca attccatttc ccctattatc cttcaataca tctaaattat
     3181 ccagtaaaaa agtatctaca ttagtatcgc caaccatagc ataaattcca gaattagaac
     3241 aacctctaac aacaggctct atgtattctt tttcggaaac tttaccaccc atgttcaata
     3301 tagtacctga tacaggtgca gcgaatatag gagaactcat ttttctacca aataattcta
     3361 tggatgtatc tggctttgaa acattatgta tgactctcat atttatcttt attttttcta
     3421 gactttttct attttcaatg aaagacgaac cacttccttt accacccata ccaggaacat
     3481 ctccagcaca agctaatcca ttacaaattt tacatacttt acatgttcca ttaaagttct
     3541 ctctagctga ttttagtaag tcattgtaat ccatacaaaa tctctccttt ttataatatt
     3601 aaaattgagt attactatat tgaaaatatc ttaattgtaa atatagggat gttattatga
     3661 tgagctaaac tagataatac atattagata ttttttattg ttgtagatag ttaaattata
     3721 tcatattaga tagtcaaatt ttgaaaaaaa ctaacttatt taagtataca ttttgaaaaa
     3781 attaaaaaag ctatatatag atttttaaag aaatctatat atagctttta agtttatgtc
     3841 aatctttttt gttaatgaat aaataatata gaatacaata tacaaaaaag tttcttgaaa
     3901 tgctaagata atccccattt acaaagcaac atcattatac tgtgtaaatt tgaatcaaca
     3961 atgttttcac catatttcat cataaaatcc tctaattgtg tgtcaataaa tgaataatat
     4021 ccacttatac tatcgatgta tgcagtcatt aagtgtatac attcatcact tttatcttta
     4081 tacatatata tctcccccta tatacaatat ataaatttag ataccaaatc gtatcatatt
     4141 aaatagttaa gtgtgtaaca tctatgcagt tatatattta tatcattata ttaataacta
     4201 aagtgagttt tgacaataga ttttgggaaa ctggatgttc aaagtgcaaa cttggatttt
     4261 aaaatcatta attaagatag cgcataggaa tatatgtata cataacttaa ttataaagat
     4321 tataaacaat tacttttatt aactttattt aaatggtaga ataacttgga gattaataat
     4381 tataaggagg atataatatg agtttatata agtttatata tgatgataag gaatatttat
     4441 taaaagagga gaactgtagt gctttaataa atgatgaaga aaatccagtt caaggagtaa
     4501 gtatttcaaa aataattgat atacttaatg aagcagaaga agttgatttt gatgtagaat
     4561 attatcaaga agcatgtcca ttatgtttag aaggagtaaa agaaaagaaa aaattctttc
     4621 cttttttaga atatcactt
//
LOCUS       DAELNY010000233         4557 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.236, whole genome shotgun sequence.
ACCESSION   DAELNY010000233 DAELNY010000000
VERSION     DAELNY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.236"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            29..832
                     /locus_tag="KPE55_003418"
     CDS             29..832
                     /locus_tag="KPE55_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494450.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            844..1974
                     /locus_tag="KPE55_003419"
     CDS             844..1974
                     /locus_tag="KPE55_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0494451.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1978..3399
                     /locus_tag="KPE55_003420"
     CDS             1978..3399
                     /locus_tag="KPE55_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494452.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDGEEDDSDKYGDIEI
                     DYSPERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPW
                     NLYLNRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEF
                     KQAIKEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYA
                     NNKKINLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTN
                     DEILEINDVRNDGYSMNSQAPIL"
     gene            3610..4119
                     /locus_tag="KPE55_003421"
     CDS             3610..4119
                     /locus_tag="KPE55_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0494453.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         1891 a          393 c          815 g         1458 t
ORIGIN      
        1 aaaataatta tagaaggaaa ggcacattat ggaaaaattg agagttattg aaatgataaa
       61 aaataatccc aatattatag ctacaataga taatccaact gatgaaatga aattgttagc
      121 tattaaagaa aatgggcttg tactagaata tataaataat ccaaccagag agatgcaaga
      181 actagcaata gataataata ttagagcaat taaatttata gataatccaa cagaagatat
      241 gatgataaaa gcagtaaatg agggatggag tatactggat tatatcaaaa acccaactga
      301 taaagttatt gaaatggcta taaatcaagc aggatgggca attaaatatg ctaaaaatcc
      361 aagtgaagaa ttacaactgt tagcagtaag aaaaaattat gattcgataa agtttataaa
      421 acaaccttat gaaagtgtac aagaggaggc agtgagaata agttatgatg ccttgagata
      481 tatagattca cctagttata atgcagaact tatagctata aaaaataatg aagcagctat
      541 tagctttata actgatttag ataaaaataa aatgttggaa tttttaaaag tgaatatttt
      601 agtgattaag tacattctga aaaaagtatc taaagatgaa ttagagagtg tattaaaaga
      661 agtattatcc aaagaagatg ttgaggaaaa gtatattaga gattttttga attgtagtgt
      721 tatagataga aatagcgaaa gtataccaat agataaaatt atgtttattt ataaatatgg
      781 aagtaagaca gcaaaaaaaa tagctgtaga tgagaaatta aagattatgt aggagagaga
      841 aaaatgaatt ttatagacac attaaaaagc gttgacttaa tattatccac tggagaagta
      901 cctttaatag taggtgaaag tggaatagga aaaacagctt tagcaaataa gcttgccaaa
      961 gaaaatgatt ggagtttaat tgttattgat ggaaacttac ttaaagaggg tgaaataggt
     1021 ggtcttccaa ctatagaatc ttatgtagga gttaattcta atggatataa gactgaaaag
     1081 aaaacaacag tgtatgctgt tcataacaaa ttaagagaaa ttgatgaaga gatatctaaa
     1141 agtaagacag ttcttttatt tatagatgag ataaatcgtt gtgagcatac agtacagcaa
     1201 gaacttatga atttaatttt aaatagagaa attaatggat ataaactgca tgatgatgta
     1261 aagattttag cagcaatgaa tccatcaagt aaatatggtt cagattttga ttatcaagtg
     1321 gtagatatgg atgcagccca agaaaatagg tttgtttggt taaatatgga gagtgaccat
     1381 acacaatgga taaaatgggc aatagatgag ggtattgaaa gaaaagttat cgagtttatt
     1441 tccacttttc cagaatatct gcataaaata aatgaagatg atgtaagagc cactcctaga
     1501 agttatgaga gagtatctaa aatttataaa gtttataaag aaaaaaataa ttctattcct
     1561 agagcagtat ttttaaatgt tgtaaagggt aatgtaggta aagttattgc agaagaattt
     1621 ataagtttta ttgaatcaaa ctctgaacca ttaatatcct atgaagatgt ttttttagga
     1681 gaatctattg atgagtctat tgtagaaaga gtaaaaaatg agagtcatac aagactttat
     1741 ctatcagcta tgaacattct aaaagactta gaattaaata ttaagaatga taaatatgaa
     1801 tcaaaccact atattaatag atttattgaa tttttaaaaa tgtatcctgt agatttaatg
     1861 ataggtatta tgaaggatat tagaaacagt tatattgaag tatataaaaa agctatagaa
     1921 aatgaagagt ttgtaaaatc atattttgaa tcttatagtt taataagggg ataatgtatg
     1981 gaaaattatt ttgataaaca agcaaaagag ctttataata aagcaagtga aattataaat
     2041 actcattcta tgctaaaggc aaatcgtgct aatgagaaat ttgatataga tataccacaa
     2101 gattttaagt atgagttttt tagtcttgta gataaagtga atttaagtct tatggaagaa
     2161 gaagataatt tttatggata ttttttgttt cagatgtcaa gagaaataag atttgatata
     2221 agtagtccta caggtgtgaa ttttaaggga gctaaatata ttatatattt taatccaata
     2281 atctttttaa cgcttgacat aaagcaaatg gagactacaa ttaaacatga aattcatcat
     2341 atattatcta tgcatttgat gagagcaaag gagttaaaag gaaagtatag tacattagca
     2401 attaatatgg caatggatat agtagtaaat aaatttctaa acaatttacc accatatgca
     2461 actaccttag agtgggtaaa tttaaaatat tccttaaaac ttgaaccata tgaacctttt
     2521 gaatattatg tagaaaagat tcagactgaa ttagatttga tggaagtgga tgaagatggt
     2581 gaggaagatg gtgaggaaga tgatagtgat aaatatggag atatagaaat tgattatagc
     2641 cccgaaagaa ctcatgatat atgggatgaa tccaatgaga tagatgaaaa tacccttaga
     2701 gaatttactg aaaaatttat taacagttct caaaaaggta aagttcctgc ttatttagaa
     2761 agtatgatat catcacttaa aaatagtaag ggtgaattgc catggaattt atatcttaat
     2821 agaataatgg gaacagttga aaataataaa aagaagacta taacaagaag aaatagaaga
     2881 caaccaaatc gtttagattt gaggggagaa cttagaagtc ataagtcaga aatagcagtt
     2941 gcacttgatg taagtggaag tattagtgat gaagagttta aacaagctat taaagaagtt
     3001 cttagtatag tgaaaaacta taatcatgaa attacaatta tagaatgtga taatgaaatt
     3061 agacgtgtat ataaagtgag aactgaaaaa gatataaaag atagaattag gataggtgga
     3121 ggtactagat tttctccagt ctttgagtat gctaataata aaaagattaa tttgttagtc
     3181 tactttactg atggtaaagg tgaaaataaa ctacaagtaa tacctagagg gtacaaaatt
     3241 ttatgggtta tttctggaag aggagataaa atttcattaa aagaacctta tggagcagtt
     3301 aaaaaactta ctaaggttaa aacaaatgat gaaatattag aaataaatga tgtaagaaat
     3361 gatgggtact caatgaatag ccaagcacca attttatagg tattcacagg agataagctg
     3421 tattaattta ttttgatatg cttgttataa aaatctaaaa aatacattaa tcaatggtta
     3481 atgtattttt taattttaat aatataagtt ttacttacat ttaagtctca aaatgtgtta
     3541 aaatataata tatagcagat agttttggca aggtggattt agttgtcaaa actatttttt
     3601 ttattaagga tggtgatgtt tttgtttaag attgatgatt atataatgta tggtatgaca
     3661 ggagtctgca aagtcatgga tataacaaat gaacgattta caaatggaat aaaaaaagag
     3721 tattatgtat tgagtcctat ttattctaat aatacagtaa taaaaattcc tgtagataat
     3781 gaaaaagtac caatgagaaa gttactttct aaaattaatg tgctttcatt aataaatgat
     3841 attcctaata tggatacatc atggatagat aatgaaaaat tgagaagtga gcaatttaaa
     3901 aaaatactca gaggtggtaa atgtgaagag ttaataaaat tagtaagaag tatagataat
     3961 aacagagagt atgtaaaatc tattggtaaa aaaacacatc aagcagatga taatataatg
     4021 aaagaagctg aaaggttatt aaatgaagaa tttgcaacta ttttagatat ttctcctaat
     4081 gaagttagtt catatatttc aagtcatata cctcaataaa gtgttttagt atcctttatt
     4141 taatctattt taagtattat ttatgtatgc tggttttggt aaaataaaat atataattaa
     4201 attatataaa aaataataat gattgtattg aagaattaca gaatagtgcg gagagcctac
     4261 tcctttaggt gtgagatgga tatcacttaa agatttaaat tgttattgta gatgttttta
     4321 atataaaaca agattaataa gtgtattgta agctttaagt gaaattgata ctataatgag
     4381 ttacttagat tttaaaatat ataatattat aaaaaatcct tttaggtttt gaatgaataa
     4441 gatggatact atgttgaata gaatccatct tatttaagtt ttatttatgt tgtttatttg
     4501 taagcattat ttttgtacat gaaaacacag tgtaaaaata tgcttagaat tatgaag
//
LOCUS       DAELNY010000234         4527 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.237, whole genome shotgun sequence.
ACCESSION   DAELNY010000234 DAELNY010000000
VERSION     DAELNY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.237"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            268..1743
                     /locus_tag="KPE55_003422"
     CDS             268..1743
                     /locus_tag="KPE55_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0494454.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1830..2243
                     /gene="speD"
                     /locus_tag="KPE55_003423"
     CDS             1830..2243
                     /gene="speD"
                     /locus_tag="KPE55_003423"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG0494455.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2273..3124
                     /gene="speE"
                     /locus_tag="KPE55_003424"
     CDS             2273..3124
                     /gene="speE"
                     /locus_tag="KPE55_003424"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG0494456.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSCYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3114..3992
                     /gene="speB"
                     /locus_tag="KPE55_003425"
     CDS             3114..3992
                     /gene="speB"
                     /locus_tag="KPE55_003425"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG0494457.1"
                     /translation="MKNNFYHRNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDAAVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4172..4372)
                     /locus_tag="KPE55_003426"
     CDS             complement(4172..4372)
                     /locus_tag="KPE55_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0494458.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1811 a          502 c          764 g         1450 t
ORIGIN      
        1 aaaaaccaaa ataataaaaa ataagaaagt ttataaatac taagttttaa aaaaattaat
       61 ataaacaaca aaaaattaaa aagtttataa atattgatat agctaatgta tatgtttttg
      121 agtaacttca aaaaaacact tgtaaaaatg ctgagatgtt aatataataa atatcatgag
      181 tttaataaaa gtaaaaataa aaaatagaaa taataaataa taaatattaa aaatatttga
      241 atatagagag gagatgaatt tgaaatcatg gatagcgagt ttgcaaatgc agttgaatta
      301 agctatgagt gtgctccatt attagagtcg ttaaaggaat actcagagaa agatatagct
      361 tgttttgatg tgcctggaca tgtaaaaaat agaggtgtag ctatattaaa taaatatcta
      421 ggtgaaagtc tcatgaaaat ggatataaat tcatcaccaa ctatggataa tgtatctgcg
      481 ccaaatggga ttataaaaaa tgcacaagat ttgctagcac aagcatatat ggctgatgag
      541 gcattcttta taacgaatgg cacaactcaa gcaatacatg caatgatttt gagtgttata
      601 aaaccaggtg aaaaagtatt acttccaaga aatatacata aatcagtaat aaatgcgtta
      661 atactttgtg gaggaatacc aatatttata caaccagaat ttgatgaaaa actaggtata
      721 agtttaaata ttactcttga aaaagttaga acagagatag aaaaagattg taatataaaa
      781 gcattatttt tccttaatcc aacttactat ggagtttgtg cagaccttga gagtattata
      841 gaactatgcc ataaaaataa tgttttagtg ttagttgatg aagctcatgg tgcacatttt
      901 ccatttcatt tagatttacc tccttctgca ataagtttgg gagctgatat ggtagctgta
      961 agtatacata aaactggagg agcattgaca caatcttcag cactcttatt gaatagagat
     1021 aatgtaagtt ttgaaaaggt tcttcaatca atcaatatgc tacaatcaac atcagcatct
     1081 tatttgctta tggcaagtat agatggtgct agagttaatc tagttgagaa tggtgaaaag
     1141 caactgtcaa aagcattaaa tctatctagg tatgcaaaat ctaaattaaa caaaattgat
     1201 ggaataaaag ttttatctac agaaatattg aaacaaaaag gtgttaaatt tatagatgaa
     1261 acaaaattgt gtataaatgt aaaagaatta aatttgactg gatttgaagt atacgattta
     1321 ttatataaaa atttttcaat tcaagtagaa ttgggagatt catataatat actggcactt
     1381 gtgtccattg gaacaaataa atcagatata gatagattag taaaagcact ttctataatt
     1441 gcaaagattt atagaaaaga atcaacatta aatgaattta atatggttca aataaatcca
     1501 attattaaac ttaatccaag agaagctttt tatgcaccaa aggaaagtgt agaaatcaat
     1561 tcatgtatag atagaatttg tggagaatca ataatggcat atccaccagg tataccgatt
     1621 attgctccag gagaattgat aacagaagaa attatggagt atattatttt cttaaaaaat
     1681 agcaatgcat atcttacaga tgtacaagat aaaaacttag atagaatatt agtaataaaa
     1741 taaattactt tattttgcaa gggcaagtat ttgttaagta gagtttatca aatagaactt
     1801 attaaaagta taaaggagtt ggggttgaaa tgaaatttga aacactagga agacatatac
     1861 tagtagaata ctataattgt gatgaaaacg tacttaaaga cccacgtctt atagaaaatt
     1921 ttatgaatga ttcagcatta aatgcaaaag cgacaattgt ggattctgta ttccaccatt
     1981 ttaatccttg gggggtttca ggagctgtta taattgctga atctcatttg acaatacata
     2041 catggcctga atatggttat gcagcagcgg atttttttac ttgtggtgat atagacccat
     2101 ggaaaagttt tgaattatta gagcaacttt taaagtctga aagaagcgag tctacagaaa
     2161 ttccaagagg cttaactaca aaaattaaaa aatattcaaa aaaagattta ggtaagatta
     2221 ctcataaacc agaagcagta taaaacatac aagagaaagt gaggattatt atatggaact
     2281 ttggtataca gaagaatgga cagaaaatgt aagattttct ataaaagtaa ataaacatct
     2341 atttgaagga aagtctcaat ttcaaaggat agatgtattt gatagtgatg aatttggaaa
     2401 atttctaaca atagatggat taatgatggt aacatataaa gatgaattta tataccatga
     2461 gatgataact catgttccta tggctacaaa tttaaacata aagaaagtat tagtcatagg
     2521 tggaggtgat ggaggtacag taagagaatt aagctgttat cctcaaatag aaaagattga
     2581 catggttgaa atagataaaa tggttgtaga tgtatctaag gagtatatgg atatatgctc
     2641 atgtaaatta gatgataaaa gagtgagttt atattttgaa gatggtgtta attttgttaa
     2701 gtgtgcacat gataaatcat atgacctgat aatagtagat tcaactgacc caataggacc
     2761 aggcgaaggg cttttctcaa ctgattttta taaggattgt tatagaatac tgactgatga
     2821 tggaatttta gtaaatcaaa gtgaaagtcc gtattttgac tttaatgcta aagagatgaa
     2881 aagagctaat aaaaagttaa aacagatatt tccaatatca gaagtttatc aagcacatat
     2941 accaacatat ccatctggtc actggttgtt tggatttgct tcaaaaaaac ttaatccagt
     3001 aaaaaatcaa gatagaaatg gatgggagaa attaagttta aaaactaaat attataatag
     3061 tgatatacat ttaggttcgt ttatgttacc tcaatacgtg aaggagatgc tagatgaaga
     3121 ataatttcta tcataggaac acttttatga gtatgaacaa aaattatgaa gaatctaatc
     3181 ttatagtatt tggtgttgga tttgatggaa ctacttctaa cagacctggg gctagatttg
     3241 caagtagctc tatgagaaaa gaattttacg gtcttgagac atatagtcct tttttagatt
     3301 tggatttaga ggattataat atatgtgatt atggagattt agaaattagt gttggaagta
     3361 cagaacaagt cttaaaagaa atctatcaag agacatataa gattgttaga gattcaaagg
     3421 taccctttat gattggagga gagcatttag ttacattacc agcctttaaa gcagtacatg
     3481 aaaagtacaa tgatatatat gtaattcatt ttgatgccca tactgacttg agggaagaat
     3541 ataataatag taaaaattct catgcaacag taattaaaag aatatgggat attgtaggtg
     3601 ataataaaat atttcaattt ggtataaggt ctgggacaaa agaagaattt aaatttgcta
     3661 cagaagaaaa acacacatac atggaaatag gaggaataga tacgtttgaa aatatagtta
     3721 atatgctaaa tggaaagaat atttatctaa ctatagattt agatgtattg gatgcagctg
     3781 tgtttccagg aacaggtaca ccagaacctg gtggtgtaaa ttatagagag tttcaagaga
     3841 tttttaagat tataaaaaac tctaatataa atatagttgg ttgtgacatt gtagagttaa
     3901 gccctgatta cgatacaaca ggtgtatcga cagttatagc ttgtaaaatc ttaagagagt
     3961 tatgcttaat aatatctgat aaaattaaat agagtatttt atataaatag tatatattat
     4021 aatataagaa gttattttaa aaaagttagt ttaagtttga cgcttttcta aagtaacttc
     4081 ttatttttgt aattaaaaat aaatataata aacaacaaaa aagagccctc gatagctcct
     4141 tttctaaaat atatatattt aaattaaata attatattct atttacgttt tcagcttgag
     4201 gtcctctatt accttgagtt atatcaaagc taaccttttc gccttcttct aaagttttgt
     4261 atccatcatt ttgtatagct gagaaatgta cgaaaacatc atcttctccc tctacagata
     4321 tgaacccaaa tcctttttca ttgttaaacc attttactat tccgtttttc attataagaa
     4381 aacctccgca ttattaaaaa atacaaacta aaaaaataag tctgttctat taatataaca
     4441 taaattttaa ataaaagcaa ataaaatttt taaaaaaatt aattactttt agaatttaat
     4501 aaaaattgtt aaattataca gtgataa
//
LOCUS       DAELNY010000235         4396 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.238, whole genome shotgun sequence.
ACCESSION   DAELNY010000235 DAELNY010000000
VERSION     DAELNY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.238"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            147..737
                     /locus_tag="KPE55_003427"
     CDS             147..737
                     /locus_tag="KPE55_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0494459.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            851..1696
                     /locus_tag="KPE55_003428"
     CDS             851..1696
                     /locus_tag="KPE55_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494460.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            1693..2376
                     /locus_tag="KPE55_003429"
     CDS             1693..2376
                     /locus_tag="KPE55_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494461.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            2373..3089
                     /locus_tag="KPE55_003430"
     CDS             2373..3089
                     /locus_tag="KPE55_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494462.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            3186..3449
                     /locus_tag="KPE55_003431"
     CDS             3186..3449
                     /locus_tag="KPE55_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494463.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            3421..3876
                     /gene="def"
                     /locus_tag="KPE55_003432"
     CDS             3421..3876
                     /gene="def"
                     /locus_tag="KPE55_003432"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0494464.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            3928..4389
                     /locus_tag="KPE55_003433"
     CDS             3928..4389
                     /locus_tag="KPE55_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG0494465.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
BASE COUNT         1713 a          412 c          708 g         1563 t
ORIGIN      
        1 tagataataa aaaatgctat ataaaattca gaattttcga aagtttttaa gcttattttt
       61 aaatatacta ataatactat tgacattaga tagaaaaagt attatattga ctatataaat
      121 tattttagtc aaactggagg gaaaagatgc ctaaatcata ttctgataaa gaaagagagt
      181 atataataaa aagacttaaa gaagaagcaa gattgtgtat ggagcaatat ggtattagaa
      241 aaactactgt tgatgagctt gtaaaacggg taaaaatacc aaaaggaact ttttatctat
      301 tttttcaatc aaaagaatta ctcttttttg aggtattaag agatatacat gactctatac
      361 aaaaagaaat cttatatgag ataaatagag tagatgaaag tattacatgt gaacaattga
      421 cagatatatt tatgaaattc tataggatgg ttgatagtac ttcaatttta aatctcatga
      481 taaatggaga atttgagata ttggtgagaa agttaccaga ttctataatt gaagaatact
      541 ttagacatga tgactttgaa attggagaga ttatatcttg cataccaaat gcaaaaaata
      601 aagacataga aagctttagt ggagcattta gagcagtttt tcttaccatg ctatataagc
      661 gcgaggttgg aagtaattgt tttgaagatg ctttaagatt aatgataaac ggattagtta
      721 tacaacttat ggaatagaat ttaaattttg tttgctaata acttttatta aaaattatta
      781 acaaacaaaa aaattatatt ttattgacca tatagtcaaa atagtcaaaa atttgaaagg
      841 gtgatattga atgattaaag ttgatgattt atcatttagt tatacagata gagatttttt
      901 acaaaatatt aactttgaag taggaaaagg ggaaatacta ggatttttag gaccatcagg
      961 tgcaggaaaa tctacattac aaaagattct aattggaatg ataactaatt atggaggaag
     1021 tgtcattgta aatggagtag agagtaaaag acactcaaat aaattttatg aaaatatagg
     1081 agttgatttt gaatttccta gtttatatga aaagttgact gcaatagaaa atttaaaata
     1141 ttttgggtct ctatattcta aaaaactact atctattgat gagttattaa aatcagtagg
     1201 actagaaaat gaatcaaata aaagggtatc tgagtattca aaaggtatga aatctcgatt
     1261 aaattttata aaagctttat tacataatcc agatatttta tttttagatg aaccaacaag
     1321 tggtcttgac ccatcaaata gcaaggttat gaaagatata attttatcag aaaaatcgaa
     1381 aggtaagact attattttaa caactcataa catgttagat gcaacagagc tttgtgacag
     1441 agtagcgttc attgtaaatg gtaaaatatc tgctttagat acaccacata atcttattat
     1501 gtcaaaagga gctattaaag ttagatatac ttactttgat aatggagaaa aaacttctga
     1561 atgttttctg aacaatactg ctaatgataa aaatttgaat atgcttattg agaaaaataa
     1621 acttttatca atacatagta gtgaaccaac attaaatgat atttttattg agataacagg
     1681 gaggaattta caatgaggct aaaaaatctt attttaggag atataaagtt tcaatttaag
     1741 tatggttttt attttttata tcttttttta agtattatat atatttgtat tatcaatgta
     1801 tttccaactt ttatgagaga aaaaatagct ataattatga tttattctga cccagcagca
     1861 atgggattgt tttttatggg agctattgta cttcttgaga aaagtcaaag ggttttaaat
     1921 tccttagctg tatctccagt aaaagtatct gagtatattt tatcaaaggt aatttcatta
     1981 ggagtaatat ctagtattgt agctatgttt attgctatta cattaaattt agataatatt
     2041 ataatatcaa ctataggtac ctttttttct tctattatat tttctttgct tggattgata
     2101 attgcaagta aagcatctag tttgaatcag tttattgttt taagtattcc tattgagatt
     2161 atatgcttta ttccaccaat actgaatgta cttcttgaca caaaatcata tgcaaatttg
     2221 taccctttta atatatgtat ttctcttatt agtggagata aaaattttat aatgattaat
     2281 attttaattt taatatcaat tataataata atttatttta taacttatta ttttatttgt
     2341 agcagttgga aaaaggtagg tggagtaaaa ctatgataaa gattataaat gcgtttaaac
     2401 aagagtttga acaaattaag agagatgcta tgctttttat agtatgtgtt tctccaatat
     2461 tatgtggagt atttataaaa tttggaatac cattaataca gaatatatca cttaacaagt
     2521 tttattatca attaaattta gagccgtatt tcttgatgtt tgatttactt ttagctttta
     2581 ttacaccttt tatgttcttt tttgcttcta caatggtcat attgggagaa attgatgaca
     2641 gcatttcaag atatttaata gtaactccgc ttggtaaaac tggttatctt atttcacgat
     2701 ttggaatacc aggtatctta gcatttatta ttactatgat tctacttatc tttttttctc
     2761 taactaaaat ttctctcttg ttaaacttat caatttcttt attagtttta ttacaaggag
     2821 ttattatatc aatattagtg atttctttat catcaaataa actagaagga atggtaatta
     2881 cgaaattctc aggtgttttt atgatgggta ttttagcacc attttttatt ttaaataaag
     2941 cacaatatat tctgttcttc ttaccaactt tttggatatc gaaggcattt aaggaagata
     3001 attatgtcta tatgtttatt tcaattgtta tatcattaat atggatttta ttattattta
     3061 aaaagtttag taaaaaaata gttaaataag aaaatttaat atataaatgt aaggttgact
     3121 tgacagtaat gttgaaaaat gttacattta agatgtaaag ttaaccttac atgaatggag
     3181 caaatatgaa aaataaaata aagatattaa gagaaaaatt aggattgaca caagaacaac
     3241 tagggagatt agttggaaca tctagacagg ctataaatgc tatagaaact ggtaaaaatg
     3301 aaccttcaat atggttggca tatgatattt caagaatttt taatgaacca attgaaagtg
     3361 tatttttatt tgaagaaagt gaaagaaaat caagagcaca gataagtagg ggagaatatt
     3421 atggctataa gaaagataag aacatttgat gatgaaattt taagaaaaaa gagtaagtat
     3481 gtagaaaacg tagataataa gataagagaa atattaaatg acatggcaga gaccatgtac
     3541 aatactccaa atggaggagg attagctgca tgccaagtag gagtattaaa gcgattagtt
     3601 gttatagatt taggagaagg acttattaag cttgttaatc ctgaaattat tgaacaagaa
     3661 ggggaacaaa ttgttgttga aggatgttta agttttcctg aagtatgggg taaattaaaa
     3721 agaccaaaaa aagttacagt gcaagctcta aacgaatacg gtgagaaaat agaaattaaa
     3781 ggttcaggtt ttatggcaaa atgtctttgt catgagatag accatttaaa tggtatagtt
     3841 tttacagata aaattattga acatgtaaaa ttgtaagata gattggttat tgaatttata
     3901 aaatatttta tagtaaggag ttaaattatg agattatggc ataaggattt aattgatgta
     3961 ttacctaaaa atcaattagt aagtcaatgg agagagctac ttgctataaa aggttctata
     4021 gataaaaaag gtactccaaa ccatctatta gtgaataaag tattgaatta tagtatagat
     4081 gaatttaaat tttatacaaa aattgtacat gatgaaatgt taaaaagaaa ttataaacct
     4141 aatgaactta aatatacaag tatattaaaa tggaaaaata gaaattttgc aaatgatata
     4201 tctaatgaac atagtttaaa tttagaaaat ttatatgatg attggcataa taaaatgtat
     4261 ttaaagcaat gcttatataa tttagaagaa aaagcaacat gtggaggaat accaataaat
     4321 gaatggaata tattactttg taaatatagt aaagattatg aattgtggtc tggaaatatc
     4381 atgttttaat tactat
//
LOCUS       DAELNY010000236         4378 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.239, whole genome shotgun sequence.
ACCESSION   DAELNY010000236 DAELNY010000000
VERSION     DAELNY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.239"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..1310
                     /locus_tag="KPE55_003434"
     CDS             231..1310
                     /locus_tag="KPE55_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0494466.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINIQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKKSRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KPE55_003435"
     CDS             1634..2002
                     /locus_tag="KPE55_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0494467.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="KPE55_003436"
     CDS             2119..2922
                     /locus_tag="KPE55_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0494468.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="KPE55_003437"
     CDS             3307..4197
                     /locus_tag="KPE55_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0494469.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT         1669 a          474 c          825 g         1410 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt gtttatataa ataaatttat atagtttata gtttaacagt aggaaatatc
      121 gtatgagtga gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg gtcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtatgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgt agaactctgc taaaaaaagg aaatcgtgat gtattagctc
      961 tttttggata tgatgatact acttcaataa atattcagga ttttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaaag agtaggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatccaggt gttaatccct
     1441 gccgattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tgcaataccc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggatagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggtgta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaagcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat taactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgcatgta tggtgtaaca
     3961 atgcgtagtg gtcagagaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtacttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tcttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gagattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtattg atattctagg taattgctag agttagtatt
     4321 atagatgaat attaatattg atattctagg taattgctag agttagtatt atagatga
//
LOCUS       DAELNY010000237         4365 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.240, whole genome shotgun sequence.
ACCESSION   DAELNY010000237 DAELNY010000000
VERSION     DAELNY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.240"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..2076
                     /locus_tag="KPE55_003438"
     CDS             58..2076
                     /locus_tag="KPE55_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0494470.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVISQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            2290..3147
                     /locus_tag="KPE55_003439"
     CDS             2290..3147
                     /locus_tag="KPE55_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0494471.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            3244..4113
                     /locus_tag="KPE55_003440"
     CDS             3244..4113
                     /locus_tag="KPE55_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0494472.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1831 a          383 c          724 g         1427 t
ORIGIN      
        1 gaataagata acaaatatga taaaattggt cttgttgaaa tttttagggg ggatatgatg
       61 acaaagaaac tagtaatatc aaaaacaaag ttgtttattt ttataatagc atttatttct
      121 atatttatat atttatttgg aagtaaaaat acacttatag gtgtaggtat agtaacagct
      181 atgctaacac tcttggaaag agatttaact atttctccaa ttaaaaattt actgaagtat
      241 ttagcaataa atataatctt aggaatacta tcattttttg cagtacaaaa tatgtattta
      301 ggagttttat taaattttat agcattattt ataatagggt atgtatttag ttatgactta
      361 aaaagagcag tatatgtacc ctttggtcta atgtatatat ttatggtaag tatacctgta
      421 ggaaaaagtg aatttcctat gagattgtcg gctttagctg ttggagcagt tataataatg
      481 attgctcaat ttgttatgaa tagaaatagg atgaaaaatg tcggagataa agaactgata
      541 tctatatgtg atgaattatt agaaaaaatt agtcttttaa aaaatactat taatgatgat
      601 agttctataa ttaaatctag tatgagaaaa atagatagct gtaattatag aataaattct
      661 atatctaaaa atttgaaaat ggttattttt gataatagaa aagatgactt ttatatcagt
      721 attagaggta tagacataat gaatatttta ttttcattag aaagaataag tttaatcctg
      781 gaaagataca aaaaggatag taaagaattt gaagatgaag atataaaaaa tattttagaa
      841 gaaagtagca taaatagtaa atcagatgtt ttagttgtag caacgaaaga aataaactat
      901 attaagatgt gtttagaaaa taaagatacc ataactgata aagagatatt aggatttaga
      961 gattatgtta tttctcagga tactaaaaat ataaatttaa aagaaattta tagtgtttta
     1021 gaaaatttat atgagttttt agtagattat aaaaaagtaa aatcaagaga cgaaaagaaa
     1081 gctgaaagaa aatcaaagat acctcatgaa tttaaacgac tatcaatata taagaaaaac
     1141 tttaatttaa attctataag attttcttat gcagtaaaga ttgctttagc tacagctgta
     1201 gcgggtttta ttatggatta tttccattta agagatggaa gatggataat gttaactgtc
     1261 ttttcattaa cacaacctta cgcagaaaat tgtatacaaa ggtctagaaa aagaatagaa
     1321 ggtacattta taggagctgt aatatttatt gtattatttt caataataaa agatagtaca
     1381 ttaaggtcgt taatagttct tttagcaggg tatataaact cttatgtagt tgattataga
     1441 aaattaatgg tatgtgtaac tgtttcagct cttggttcag cagtagttat gggagaccca
     1501 aatgttttaa caataagcag gatttcatat gtagcattag gagcaattat tgcattaata
     1561 gtaaataagt ttatattacc atatgatgct aaaacaggct atcaacatgt tatagaaatg
     1621 tataaaggaa tagttaaaaa tataattgat gaagtcaata agtctataga aaatgtagct
     1681 gatgtttact atataaaaaa tttattattg ataccatcac ttattgaaga tagacttatg
     1741 ttgataaatg ctatatataa agataaacat caagaggatt ttttagaaaa tcagaaatta
     1801 ctaataagta atatgtataa tctatatata aatgttaata aaaataaaat aaaagatgaa
     1861 gatgttgaaa aaatattgag agataccaat tatatttcaa attacaatgt agataagtat
     1921 gatgaaggaa gaagtgtaat tttagagagt attgtaaata ctaaaagtct aggtgacaag
     1981 ataatatgtc taaacttact gcaaactcta aatggtgtca aagaaatgta tagaataagt
     2041 aatataacta aagtttcaat aaaagaagca gcttagatat taaattatag aatagccata
     2101 tatttaaaat aaagtatatg gctatttgtt ttataaaata ctaaaagaaa ataatatata
     2161 aaaaacaaaa acatatattt atgaatttta tatgaaaata gttaaaatat tgattttttt
     2221 cattggttga aaaaacaatt tacttaggat aaactataat agtattcaaa tattatgggg
     2281 gttagattta tgagtaaaga tgtaaaggta aagcgtaata agaaatatga tacatttagt
     2341 ataactatgg aacttgagag acctttactt tatgatgagg ttgagaacaa agatgataaa
     2401 aataataata gaaaatttaa cataaagaat aagatattta agggaatagg tatactattt
     2461 gtattgattt tgcttggctt tttcatatgg ataaataaaa catatgaacc tcaaaagctg
     2521 gcaaaagaag ctctagtcag caatagtaaa gttgaagtaa ctgtaaatga aaacatttca
     2581 tttacaccaa aaggaagaaa agtttctaaa ggtttaattt tgtatccagg agctaaagta
     2641 gaaatagaat cttatgctcc acttgcaaga aaaatagcag aaagtggata tgaggttgta
     2701 atcgtaaaga tgccacttaa tctagctata cttggaacta ataaggcaca gaaagttatg
     2761 gattcttaca acaacataga acattgggta ataggaggtc attcattagg aggagttgca
     2821 gcatcaaatt ttgcacgtga taataaatta attgaaggcg ttgtattttt agcttcttat
     2881 ccaatgggtg atgagttgaa agaattagga aaaaaagtta tatctatatg ggggagcaaa
     2941 gatggagtag tcaattttaa aagtcttgta gaatcaaaac aaaaacttcc tgatgataca
     3001 acttatgttg agattgaagg aggaaatcat gcacaatttg gagattatgg aaaacaaaag
     3061 ggagataatg atgctattat aagtcaagaa aaacaactta atattactgc caatagtatc
     3121 attaaatttt taaagaatat atcttaaact aaaatttaaa aaatataatt ttataagtta
     3181 ttatttaaag taaatattaa taatataata gaatgaatat tttattacaa gggggatttt
     3241 aatatggaat acgatatgcc actttacaga ccaccaagtg aggcttatag tcttataata
     3301 caagtcacac taggatgttc tcataacaaa tgtacgtttt gtagtatgta caaatctaaa
     3361 aaatttaaaa taaagccact agaagtaata aaaaatgaaa ttgatatatt tagaagatat
     3421 tataagcatg tagaaagaat attcttagca gatggagatg cattgataat accaatggag
     3481 aaattaagag atattatttt atatataaaa gaagtttttc cagagtgtga aagaattact
     3541 atgtacggct ctcctaaatc tatagagaaa aaatctgatg atgaacttaa agaattaaga
     3601 aaacttggtg ttaaaatgat ttatctaggg cttgaaagtg gaagcaatga agtattagaa
     3661 gatattaaga aaggttttag tagtgaacaa ttaataaaag ttggtagaaa agttaaaaaa
     3721 agtgggatta aattatcagc cactgtaata gcaggtcttg gaggaactaa aaaaacacat
     3781 cagcatgcag ttgatacagg aaaaatgtta ggagcaattt caccagatta tgtgggtgta
     3841 cttagtttaa tggtagaacc taatactgaa ctttacgatt tacttaaggc aggagagttt
     3901 acaatactag aggataaggc tgtgttacaa gaaattaaag agatgataaa gaatatagat
     3961 gctaatgaaa aaattgtatt tagaagtaat catgcatcaa attatgctaa tttaaaaggt
     4021 gtattacctg atgataaaca acgtctaatt gatgaaattg attactatct aagtaaccaa
     4081 aagctaaaaa gagaagaata tagaagacta tagtttttga tatatcaatc tatattgata
     4141 ggtaaatact aagattattt agatacaaaa aatcatgcta cattaatagt tgcttactat
     4201 tgatgtagca tgattttttt attaaaaaac aaaatggtat attcatatat aatatatcaa
     4261 taataatgaa aatgtgaact agtttaatag aattttacct attataataa aaacaggaat
     4321 ttaaataata aaaagtaaag taacttttta ttaagtaact ttact
//
LOCUS       DAELNY010000238         4343 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.241, whole genome shotgun sequence.
ACCESSION   DAELNY010000238 DAELNY010000000
VERSION     DAELNY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.241"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..367
                     /locus_tag="KPE55_003441"
     CDS             68..367
                     /locus_tag="KPE55_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494473.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            691..2346
                     /gene="ilvD"
                     /locus_tag="KPE55_003442"
     CDS             691..2346
                     /gene="ilvD"
                     /locus_tag="KPE55_003442"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG0494474.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKYVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2690..3322
                     /locus_tag="KPE55_003443"
     CDS             2690..3322
                     /locus_tag="KPE55_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0494475.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            3345..4157
                     /locus_tag="KPE55_003444"
     CDS             3345..4157
                     /locus_tag="KPE55_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0494476.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWINMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1683 a          488 c          796 g         1376 t
ORIGIN      
        1 gtatgtataa atattataaa tatatggaaa taataatagt atattttggt aaagtaggag
       61 gaattttatg aaatggatta aatggtatag tattacatgc atatgtattt ttatagtagt
      121 agctttttat atgtttatat ttcctaataa aatagaaact attgatactt catctgcata
      181 tagctttgtt gaaaagaaag taccaaatag tgctgtgtat caaggataca aaaagaatcc
      241 agttgatggt actactacaa tttattacag ctatgataat tcaactcata tagttagatt
      301 atcacatcca gaagactatt caaggaagat taattgggat aaagtttcaa acatcagatt
      361 tgattaatct ttaatattta taaaagtcta tatatctaag gcaaatatat tattaatctt
      421 attcatagtt gagcactttc tattatagaa gtgcctcttc tgatttatat cataaaatat
      481 tacttaacaa tttgtaaaat atattagttt ataaaatata ttgaaaagat aatattatat
      541 aatacgattg tgtaagatat tgataaaaaa tcgtaataaa ttttcattag tatatgctaa
      601 tattttttta caattttcgt aattaattaa tagaaaaata tattataata atattatgaa
      661 aatttaaaaa agtaagaaag aggtaatatt atgagaagtg atataaaaaa aggaatagaa
      721 ggagctccaa agagagcgtt aatgtatgga atgggtctta ctaaagaaga aattgaaaga
      781 ccattaatag gtatagtaaa tgcacaaaat gaggtgattc ctggtcatct gcatcttgat
      841 gaaatagcag aagctgcaaa aaatggagta agaatgagtg gtggtttgcc attagaattt
      901 cctgctatag gggtatgtga tggtatagct atggggcatg tcggaatgaa ttattccctg
      961 gcttcaagag aattaatagc agattcaata gaagctatgg ctatggcaca tggttttgat
     1021 gctttggtac ttatacctaa ttgtgacaag atagtgccag gtatgttaat ggctgctgca
     1081 agacttaata taccaagtat tgttgtgagt ggagggccta tgcttccagg taaaaaaaat
     1141 ggaaaagtat atgattttaa ctctgcaatg gagggtgttg gagcatgtaa agatggaaca
     1201 gtaagtgaag aagaattaga agcgttagcc atgaactctt gtcctggatg tggttcatgt
     1261 tctggtcttt tcacagcaaa tagcatgaat tgtttaacag aagctttagg catgggtata
     1321 ccatataatg gtactgctgc gtcacattct ggagaaagaa aaaggatagc aaaatatgca
     1381 ggtatgtatg ttatggagtt acttaagaac gacataaaac ctagagatat tttaacaata
     1441 gatgctttta aaaatgctat agctgtggat atggcaatgg ctggctctac aaatacagta
     1501 cttcacttac ctgcaatagc ttatgaatca ggaatagagc ttaacttaga tttttttgat
     1561 gaaataagtg aaaaaactcc ttgtttaaca aaattaagtc caagtggaaa acatcatatt
     1621 gaagatttac atatggcagg aggaatacca gctataatga acgaactttc aaagataaat
     1681 ggaataaatt tagattgcaa aaccgtaaca ggcaagacta taggggaaaa tataagaaat
     1741 tgtgaaatag aaaatgaaga agtaatacat acattaaaga atccatatag taaccaaggt
     1801 ggacttgcaa tattgaaagg aaatcttgct ctaaatggag ctgttgtaaa aaagtcagca
     1861 gttgcagaag aaatgttagt tcatgaagga ccagcaagag tttttaattc agaagaagaa
     1921 gctgtaaatg ctatttttgg taaaaaaata aacaaaggtg atgttatagt tataagatat
     1981 gaaggtccaa agggtggtcc aggaatgaaa gaaatgctat ctcctacatc agcagttgca
     2041 ggaatgggac ttgataagta tgtagcactt cttactgatg gtcgtttttc aggagcaact
     2101 agaggagcat ctataggcca tatttctcca gaagctatgg aaggtggttt aatcgggtta
     2161 gttgaagaag gagatataat ttctataaac ataccagaca aaaaattaga gttaaaagta
     2221 gatgaagttg aaatagaaaa tagaaaatta aaatttaaac ctttagaacc gaaaataaag
     2281 catggatact taagtagata tgctaaactg gtaacatcag caaatacagg agcagtttta
     2341 aaatagtaga ctatatttat gtaaattaga aaaagttaag aaaatattaa ttggatatct
     2401 taggtgttat agcactaatg aggtatcctt tttattttgt atgaaaaaca cagcaaaatc
     2461 ttgattatct ttgaaaacaa tacatctcta ttataaggaa tacgagtaca gcatagagat
     2521 gtatctttta gaccttgttg agggaataca actttttaaa agtttgatgt ggaatgcttt
     2581 tcgttccatc taaactatga agaaattgaa agtcagttaa ttcttgacat aataaatatt
     2641 aaaatgtata atgaaaaaca aacagacagt ctgtttttag gagggaaata tgaatagaga
     2701 agaaaaaagt aaaaatagta aagaaaaaat tatccaatca gcattttcac tattttcgtc
     2761 taaaggatat gattcaacat ctacacaaga tattatcaat ttatctggtc tatctagagg
     2821 tgcaatgtat catcacttta aaactaaaga agatatactg agaagtgtca caaaagaact
     2881 ttactcacaa atgaataatt ttttagagca tcttgttgct gatgacaccc ttacagcaaa
     2941 tgaaaaaata atagaattgg ttgttcatag tgcgaatgat tacacacgta gaaaaatggt
     3001 acattgtagc tggttagaaa aaatcccatt cgctttaata gaggaagttc gtaatctcaa
     3061 caatgtagtt gcacccaata ttgctaagat aattaaacaa ggtgttgaaa ataaagaatt
     3121 ttcttgtgaa tatccagagg aattagctga aatgctcgtt tttagtattg atattttact
     3181 tgaccctgta ttatttaaac gtgaatacag tgaagtatgt aataggttgg attttttatt
     3241 attcatgtta aaaaaaatgg atattcctct aattgatgag tatggtattc aaaaatttaa
     3301 agatttattt agacaattat aataggaaaa gcgaggaata gactatgttt tataatgcaa
     3361 aaagttgtca aatgatgata ggaagtaata cagtgaatta tattgaattt ggtagtggta
     3421 agaagacact aattatttta ccaggattag gagatggttt atttccttta catggaaaaa
     3481 tacaggcgat tgcttttgct tttagatata agcaatttac aaaagattat aaggtttatg
     3541 tatttagtag aaaaaatcaa attacagaaa aatattccac aagggatatg gctaaagacc
     3601 aagcagatat aatgaaaaaa cttggaatta tgaaagcaga agtaatgggt gtttctcaag
     3661 gtggaatgat agctcaatat ctggcaattg attatcctga gttagttgag aaacttgtgt
     3721 tggcagttac ttcttcaaag caaaatgata ctatccaaaa tgttatttgt agttggatta
     3781 atatggcgaa gaagcagaat tacaatgatt taatgattga cacagcaaag aaatcctatt
     3841 ctgaaagata tttaaaaaaa taccaattat ttattccttt tttaggaaaa gtaggtaaac
     3901 caaaggattt taaacgattt attattcaag cgacctcttg catagaacat aatgcctttt
     3961 ccgaattaaa taaaataaca tgtccaacct taattatagg aggtgctaat gataaaattg
     4021 ttggaaataa tgcttctttt catttagtag aaaaaattaa aaaaagcgaa atatttatct
     4081 atgagggatt gggacacgct acatatgaag aagcacagga ttttaatgaa agagttttag
     4141 agtttttaaa taagtaaaca cctttgttaa aaatatacat atgaagattg gaaatttaat
     4201 gttaaaaata gaaacatgaa aatatgctta actggtattt ttactattca taaccaattt
     4261 ttgatacatt atctactata aatacaaata tagcttcaat gtgattatat attgttgtat
     4321 tggtaaagca cttatacaaa cag
//
LOCUS       DAELNY010000239         4214 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.242, whole genome shotgun sequence.
ACCESSION   DAELNY010000239 DAELNY010000000
VERSION     DAELNY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.242"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..1674
                     /locus_tag="KPE55_003445"
     CDS             76..1674
                     /locus_tag="KPE55_003445"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG0494477.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1913..3958)
                     /gene="bclA1"
                     /locus_tag="KPE55_003446"
     CDS             complement(1913..3958)
                     /gene="bclA1"
                     /locus_tag="KPE55_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG0494478.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         1238 a          774 c          650 g         1552 t
ORIGIN      
        1 acaaatactc actacctttt gatataatat ttttatcaat tattattata ttcaagtttt
       61 gaaaggagta ttaaaatgag tttattagtt ttcggacata aaaatccaga tacagattca
      121 atatgttctg caatttcatt agcacattta aagaacaaac taggaataaa agcaacagct
      181 tatgctttag gagatatcag aaaagaagct aaatatgcct tggattactt taaggtagat
      241 gcacctgaaa ttttagataa tgttagaata caagttagag atttaaatta tgataaagta
      301 gtacctttaa caccaacttc atctatatta gaggcttata accttatgga tgaaaaaaat
      361 gttaaaacac tacctgtaac ttatgatgat ggaacttttg ctggtatcat tactatgaaa
      421 gaaatagcta aaaatctact tcatcaacac tttacaacta tacacacatc actatcaaac
      481 atttgtaaaa accttaatgg tactattctt gtggactgtg gtgaagatat aaaaggtaga
      541 gtagtcactt tatcatttgg aatggagact ctaatagaga ctttaaaaga aggagacata
      601 gctattgttg gagatagata tgatagtatt gaatatgcta ttgatacaaa agttaaactt
      661 ttaatactta ctaatcatac agagatttca aaagatttgc tagctcttgc taagataaat
      721 ggagtgtctg ttgtttctgt tgaaagtgac aattataaaa cttctaatgt aataaatcaa
      781 tgtagtttct tagactctat agaagctaca gaaaatttaa ttacattcaa agaagatgat
      841 tatatggatg aaataaaaga gattatgcta gaaacaaact tcagatctta tccaatatta
      901 gatgatgata ataagttttt aggtttagtt tcaaaaggtc atctattaaa cccatctaaa
      961 aagaatgttg ttttagttga ccataacgaa tatgctcaaa gtgctgatgg aattgaacaa
     1021 gcaaacatag ttgaaataat tgaccatcat aaacttggtg gtatttctac tgatgttcct
     1081 atgtcattta gagttatgcc tgttggatgc aatagtacta taatttatca aatgtacaaa
     1141 gaaaataatg ttgagatacc ttatgaaata gccgggcttc ttttatctgc tatattatca
     1201 gatacattat tatttaagtc tccaacaaca actgatatag ataagaaagc ttgtgaagaa
     1261 ttaagtaaaa tagcaaaagt agacatggaa aaatacgcta tggatatgtt taaatgcggt
     1321 acttctttag atgaatatac tatagaagaa atagtaaata tggactttaa agaatttaat
     1381 atgagtggac atagagttgg tattggacaa gtatttacct tagacataga ctctatattt
     1441 gctaaaaaag atgagttttt atcttatata aattcgactg attatgacaa actaattctt
     1501 gcggttacag atataatcaa agaaggttct tatttaatct ataaagcaga agacagctta
     1561 attgctaacg catttggtgt tgaaggtgtt caaggtacat ttgcaccagg tgttgtatca
     1621 agaaaaaaac aattagtccc taatttaact actgcaatca aaaattttaa ataatataaa
     1681 atttttaaat aacgaaaact gtctcaaatc tatatttgag acagtttttt attatatata
     1741 tatatattga taataaagtt aaataaaaat ccataaattt aaaattttta tgaatatatt
     1801 ttattattat tgttatttta ttcattctaa aataggtaaa actcaatata taaaaatact
     1861 tttgtcattt aaataaagcc taaatagttt ttaagattat tttagacacg ttttaagtca
     1921 taacagtatc agctattcta aatatcgtca aagtagccgc agttcctgct gattgcccat
     1981 ttcgtatccc tactgttgta gatgaaaaat tatttattgt aaaagtagtc ccaccaaata
     2041 aaaaaccagc aaaatatcca gaaagtactc ctccgcctcc tggctcagta gtaagccctg
     2101 ccaaatcttt tcctcctaaa attgggaata ggatagcgaa agaatattga tttatacttg
     2161 atgaacctgg ttgtaatgct actgaaactg atatataata cacaccattt tcttgtactg
     2221 taaacgtacc atttgcatct actgttatac ttgaactatt tcctattatt gtttgccaac
     2281 ctgttatagc atctccatta tttagtgttt gtggagatgg tgcatttacg aggaagttag
     2341 caactggctg agaggaattg cctgttgctc ctgttggacc tgttgggcct gttactcctg
     2401 tcgctcctgt tgctcccgtt ggtcctatac ttcctgtcgc tcctgttgga cctgttactc
     2461 ccgttggccc tatacttcct gtcgctcctg tatttcctgt tggccctatt tctcctgtca
     2521 ctcctgttgg gcctgttgct cccgttggtc ctatttctcc tgtcactcct gtatttcctg
     2581 tcggacccgt tgctcccgtt ggtcctattt ctcctgttac tcctgtattt cctgttggac
     2641 ctgttgctcc cgttggtcct atttctcctg tcgctcctgt tgggcctgtt gctcccgttg
     2701 gtcctatact tcctgtcact cctgttgggc ctgttgctcc tgttggtcct atttctcctg
     2761 ttggtcctat ttctcctgtc gctcccgttg gtcctatact tcctgtcact cctgttgggc
     2821 ctgttgctcc cgttggtcct atttctcctg ttggccctat ttctcctgtc actcctgtcg
     2881 ctcctgttgg acctgttgct cctgttggtc ctatacttcc tgtcactcct gtcgctcctg
     2941 ttgctcccgt tggtcctatt tctcctgttg gtcctgttgc tcctgttggt cctatacttc
     3001 ctgtcactcc tgttggacct gttgctcccg ttggtcctat ttctcctgta tttcctgttg
     3061 ggcctgttac tcctgttggt cctatacttc ctgtcactcc cgtatttcct gtcgggcctg
     3121 ttgctcccgt tggtcctata cttcctgtca ctcccgtatt tcctgtcgga cctgttactc
     3181 ctgttggtcc tatacttcct gtcgctcctg tacttccggt tgggcctgtt actcccgttg
     3241 gccctatact tcctgttgct cctgtatttc cggttgggcc tgttggtcct atgcttcctg
     3301 tcgctcctgt acttccggtt gggcctgtta ctcccgttgg tcctgtactt cctgtcgctc
     3361 ctcttggtcc cattggacct gtaattctaa ttggaagaca ctcacattta ggataacaat
     3421 tatctataat tctatcttca aatgttacaa gtatatacgg tttgttacaa cctctactag
     3481 atgtaaattc aagaatctga taagggtaat ttaaagataa agctaaccca taattaggat
     3541 atttattcat tgcccatgct tttatagttc ccgtaatatt taaacaaata tagttggatg
     3601 tatcacttat cccaactggt aaatatcccc taatattttc catagacact ctatcattcc
     3661 atgttatagt tcgtaaatta taatattcac ttattctttt aatttcaata tctacttttt
     3721 ctttaaaaat atgattctta tttgaatcaa tatgaataaa taactctgct tttaatatac
     3781 ttttgtcttt taatatatct ggtattctca ctttaaaaaa agtaatcaac ttttctttta
     3841 caaaactatt tgaacattta cttcctatta agcaataatc taaattacta aagtttttat
     3901 ctggatattt tttagatata aaagtatcat catttaaata aagtataata tttctcattt
     3961 ataatccctc cattttgggt ctatatcata ctatgtttaa aatacatgct tagttacaat
     4021 ataattaaaa tttatagtat taaattttta tataataatg ctgatttcta caaaaaaaga
     4081 acatgtcatt agaaatttct aatagcatgt tctttttcat tattatatat tacactttaa
     4141 attattcctt gactggctta gctggtgtaa actaaaccag tctttctaaa atattaagca
     4201 cttttttctt atta
//
LOCUS       DAELNY010000240         4197 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.243, whole genome shotgun sequence.
ACCESSION   DAELNY010000240 DAELNY010000000
VERSION     DAELNY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.243"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..207
                     /locus_tag="KPE55_003447"
     CDS             <1..207
                     /locus_tag="KPE55_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG0494479.1"
                     /translation="CLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIKE
                     KFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(361..1422)
                     /gene="ytvI"
                     /locus_tag="KPE55_003448"
     CDS             complement(361..1422)
                     /gene="ytvI"
                     /locus_tag="KPE55_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0494480.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            1696..2436
                     /locus_tag="KPE55_003449"
     CDS             1696..2436
                     /locus_tag="KPE55_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG0494481.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILRELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            2448..>4197
                     /locus_tag="KPE55_003450"
     CDS             2448..>4197
                     /locus_tag="KPE55_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0494482.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSS"
BASE COUNT         1791 a          367 c          653 g         1386 t
ORIGIN      
        1 tgcttaatgt taattttatt ttctgtagtt tataatgctg aatttttttc tgtaggatat
       61 ataaaagaag tagagaaatt tattccatat acaatagatt attcattaga gattgaagaa
      121 aaaataaaag aaaaatttaa aaatatagat gttttaaata atgaaaatga atataaagaa
      181 atgattgata ttatttgtgc agagtagaaa aaaagggagt gtcttagaat agacttttta
      241 gtttatgagg cactcttttt gtataaaatc aaatgtattt tcatcatttt aacaattaaa
      301 aagaacttat ccctatttga gaaagccctt tttaatttaa ataaaaatgt caaggttaaa
      361 ctatttaaat gcagtaataa tcccattatc atgtaagttt tttatgattg tagctgcgat
      421 agggaatcca aataatccag ctattccaaa tagttgagcc ccaacaaaca tacacattaa
      481 tgttattaaa ggatataggc caatttggtg tcctactacc tttggttcta atatttgtct
      541 aacaacggtt ataattatgt atagtattaa aagtcctatt gctaaattaa tatttccatt
      601 aatgagtgag ataatcatcc aaggtgtcaa aatacttcct gtacccaaga ctggcaaaat
      661 atcaacaagt gctatgatga cagatatagt aaatgcattt tcaaccttta ggatagtaag
      721 tccaataagt agttctatga atgtcactga tagtagtatt gcataggctt ttagtagttt
      781 taaaagagca ttaatactgc tattttttat atttattatc ataaattgac ttttttctgg
      841 aaattgtttt aagataaagt ttactatttt ttgatagtca aaagtaaaga aaaaagaagc
      901 aattaaagta ataataagtt ttaacataaa tgcaggtgtt ttacttgcaa tactagcaat
      961 agcattgagt gcatttgttg aaatagaagc tactaaagac atcatagatt gacttatatt
     1021 atcccaacca agtattaatg ctaggttaac attaggtgta ctagcatcta tctttgaaaa
     1081 taaagtattt agcgttggtt gtatacttga tttatatatt tcaggaagtc taaaaaatac
     1141 atcacttaca cttgcaaata tcttcacacc aaagctaact aataaaaata tacacaaccc
     1201 ataaaaaatt aaaagtatta ctatagaaat aagtgtacga tttaaatttg tattatttgt
     1261 aataagtctt ataataggtc ttaagatgga tgcaatgata aaactgatta caaaaggcat
     1321 taataatggc aataagaatt ttaatacaat atatacaata cttgttatta aaactacata
     1381 tgtaaattta attataaaat tcttttgctt ttgaatatcc ataatataaa acctccaatg
     1441 tatttgatat tattctaaat atggtctttt ttacagaaaa tatactactg tatattgatt
     1501 ataaactata aagcttatgt aaagtaaata aattatgtaa aatttaggtt aattttgtat
     1561 aaagagatac ttttatattt tacaattaat aaccaaatac taaaaaatat tttaaataaa
     1621 gaacttaaaa ttgtgataaa attaatttat agatattaaa aaaagtataa tatatggttt
     1681 ttaagaggtg ctaagatgaa aaattttaga gatttaggtg ggaacaaaac tgaggatggc
     1741 agaactgtaa agaagggatt attttatagg tctgcaaaat tatcaaatct aagtgaaaat
     1801 gatataaaaa ttttaagaga gttaaatatt aaatatatat ttgattatag aagtgatgaa
     1861 gaagctcgta aacatccatc aacaataata tcaaatataa aaaatataag aataccagca
     1921 atgagagagc tagaagaatc aggaggaagt tttggttcta ttgaagatat gattgatgga
     1981 ttgtttgaaa aagatggtgc atttaatatg ttaaataata gttactataa tttgcctata
     2041 aataatccgt catataaaaa attagtagaa cttataagag actattctaa tttaccaata
     2101 ttaaatcatt gtacagctgg taaggataga actggtgtgg ggagtgctat aattcttatg
     2161 attttagggg tatcgagaga gaatataatg aaagattatc taaaaagtaa tgattttgca
     2221 gataaagaga tagaaagatt tatagaatat aagcctaaat ttaaggacat tccaaaagaa
     2281 aatttgaaat atatatttgg tgtaaatgaa gagtatatga aaactgcatt tagaagaatt
     2341 gatgaagaat acattagtgt agaagcatat ttatatggag aatttaattt aaataaagaa
     2401 gaaataagaa aattaagaaa tcaatatcta gagtaaaagg tgagtttatg agtttaaata
     2461 taacagcaag acagataaat ataataaaaa tattgttaaa ttcaaaagat acaattagtg
     2521 gtattgcctt aagtcaagag ataggatgtt cttcaaaaac tatacaaaat gaaataaagg
     2581 atattaataa acaacttaga aatggtaaaa ttttgtcaat aagaggagtt ggttataaac
     2641 tagaaggaag ttttgatgag gtaaatctaa atagcaatct ctatgatgat gtagatagag
     2701 tagaatatat aatcagaaaa atattaacct taagtaatga agaaaaaaat actataaaac
     2761 ttgaagattt agccgattct atgtatgtaa gtgtttcaac agtaaaaaat gatttaaaag
     2821 aagtaaaaag aatgttggaa aagtattcaa tatcagttaa atctaagcat aaacaaggta
     2881 tatgtgtttt ggaaagtgaa gataagatat taaattgtat agttgactta agcaataaga
     2941 gagataatca attgagttta aatgattttt taaatgatga tataaaggaa agaaaactat
     3001 taataaaaaa attattatta gacatattaa atgaaaatgg attagtatta actgatatag
     3061 aatttaagag tgtgttaaat aatatattag ttatgttaag cagacataag tataatgaaa
     3121 aagaatttat agagaattat atagaaaatt ataggagcaa aagaagctca attttagaaa
     3181 atgaacaaaa caaagaaatt ataataaatt caataaaaac attttgtaaa aatttaaaat
     3241 tagctacatc aatagatata agtaatgata aggtatttga agagttttta tataagcata
     3301 ttagtagttt atgtaaaaaa ataaagttag gtataaatca atcacctata atggctcaag
     3361 atataaaaat aaaatatcca tttgcttttg aactagctaa tatagctaaa aagacaatag
     3421 aaaaagattt gaagatagaa attaatgaag atgaagttgc aaacatagct ctacatatag
     3481 gaggagcagt tgaaagagca tcttataata ataaagataa ggttttcaaa actataatag
     3541 tttgtacttc aggcataggc acatcaatgt taataaaagc taaattagag aatatattta
     3601 aggaaaaatt agaaatacta aagattattc cttcatactt agttgaatat ataaaagtta
     3661 tagatgttga ttttgtaata tctactgttc ctattgaagt aggagatgtg ccagtaataa
     3721 atatatctcc aattttaagt gagaaagaaa ttagattaat tgaaaaatat atagaaacag
     3781 gaaatatata cctagattta gatataaaaa gcctgtttga tagtgaattg ttttttactg
     3841 atttggtgtt taaaaggaaa gaagaagtta tagattttat ggcaagtaaa cttgtacaaa
     3901 agggctatat agatgaggat atgaaaaaat cttactttga gagagaaaaa atagcaacaa
     3961 cagaaatagg gaatatggta actataccac atggagctat tggaaaaatt tttgataata
     4021 aaatagcaat tggtatatta aagaagccta tttcttggga aatatcagat gttcgtttaa
     4081 taataatgtt agcccttgat aaagataaaa tattagatta tgaagtaata ttttcaaaaa
     4141 tatataaaag agtagattct atagcaaaag ttataagtat atgtgaaaat agtagtt
//
LOCUS       DAELNY010000241         4192 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.244, whole genome shotgun sequence.
ACCESSION   DAELNY010000241 DAELNY010000000
VERSION     DAELNY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.244"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(367..1434)
                     /gene="rsgA"
                     /locus_tag="KPE55_003451"
     CDS             complement(367..1434)
                     /gene="rsgA"
                     /locus_tag="KPE55_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0494483.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYYDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKAEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            complement(1431..1832)
                     /locus_tag="KPE55_003452"
     CDS             complement(1431..1832)
                     /locus_tag="KPE55_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG0494484.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2628..2724
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2778..2954
                     /locus_tag="KPE55_003453"
     CDS             2778..2954
                     /locus_tag="KPE55_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494485.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3364..4002
                     /locus_tag="KPE55_003454"
     CDS             3364..4002
                     /locus_tag="KPE55_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494486.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
BASE COUNT         1570 a          614 c          529 g         1479 t
ORIGIN      
        1 tatattattt gtattttata attcatatat ttttacttta taaattagca atattgcagt
       61 tttgctctta agaaacatct ctatcataca taagcatata ctctattatg tattagtata
      121 aatatgcctt ttcttatctt acataagacc tctttataaa ggagcctatc gtaacaaata
      181 tgtatacatt atcacatgta tatatcatca tataaaaaat caaactttat taaaaaaatt
      241 aataaattgc tttaaataat accacaaacc aaatttgcca tcaaaatcac attaaagtaa
      301 caaattgatg gcaaatttaa tatattaata gtatatagac tcatagaatg aacaaaaaaa
      361 ctacacttat cttctaatcg tcttatttat ttttgagata tttttaaatt tcttttcttt
      421 ttttgctaag tattcgcttt tattgtctgc aaatactgct tcagctttaa gcctacgata
      481 ggactcccat cttttaggcg aaagttcttg atgagcaata gcttcccaga tagcacagcc
      541 tggttctgag gtgtgagtac aattacgaaa atgacatttt ccgaaataag cttctatatc
      601 tgcaaaactt ttatctaaac cttctgaaac atcccacatt cccaactctc tcatacctgg
      661 tgtgtcaata acaataccac cacctgaaat taaaaatagc tctcgtctag tcgtggcatg
      721 tcttccttta tcatcatcta tgcgaatttc ctttgtattt aaaaattctc cacccatcag
      781 acaattaatt aatgaagatt ttccaactcc tgaagaacca agtaaggcaa cagtacagcc
      841 tggtttaata tacttagaaa tctgtttata accatctggc tctatagatg aggtaacaac
      901 tacatctaca cccaaggcta tagaattaac tacttgtaat ttacgatgca cattgtcaca
      961 taagtctgat ttagtaagta ctactactgg gatagctcca ctttcccatg ttaaagacag
     1021 atacctctcc aatctatgaa tgttaaaatc cttattcagt gacatgcaca aaaaaacaat
     1081 gtcaatatta gctgcaacta cctgctcctc atttaccttt ccagcagcct tacgcacaaa
     1141 caagcttttt ctatgaagca catgatgaat aatagcatta ccatttttat tattgcaacg
     1201 atccaacata acgaaatcac caactgtggg aaaatcagat tgtatagtag cctcaaaacg
     1261 cagctttcct gaaacctctg caaacagtac accatttaca catacagctt gataaaagtt
     1321 cttttcctgc gagataatcc gagcaaggta taaatcataa tattccatac tttgagtaat
     1381 aaaattccta tttagaccat agtcttgtaa attgatttgt ttcataccat tcattctgtt
     1441 tcctcctttt ccaacttttc aatgattctt tctagtggga aagggggctg tgccagtggt
     1501 tttaatgcaa ttgacattaa tttcactgaa ttatcatcag ctgcaatacg attagaacta
     1561 agatgtccac agcgtgtaca acgatgaata attgcccact caccattttt tctcacccac
     1621 actccaatag gttccatatt agcatcacaa tctgcagcac ggtcaccagg aatgatatct
     1681 acatgaagac tatgtaaaca atgtgggcaa tgatttctat gttgtgtacc agctccttca
     1741 ggaactacaa ggcaaccaca atttttgcaa ataaagttat ccatacagga attttcctga
     1801 taataagctc gttttctatt tttataattc atttttattt cctccgtatc ttgtattttc
     1861 tacaaaaaaa gtccacaggt atttatatct gcctttttat gtggtataca tatttaccta
     1921 cacaaaaaca gaaaacccct gtggactaga aaatttaata tcttctattt caacataatt
     1981 atattcaaaa taatactgtg taaaattaat aaaagtgtag cagattaaat cccactacac
     2041 cacaatttaa caaagtaata tgtaatatta ttatttgttg tctaatgtta gttatctata
     2101 aaggcagagt aagacacaac aatcaaatgt atcaaaaata cacttaaaaa ttctgattaa
     2161 acagaaccca tatgcgtctc caactattta tttgttggtt accattatat ataactctga
     2221 catcaggcaa gacactcctt cactaataat tattattgaa attttatcat aaaatataag
     2281 tatgttcaag tataatttaa tatattcata tatttatgtt tgtctcattt tcttattcta
     2341 taaaatctag tggtatttta aaatttaatg ttcacctagt attaataata aaaattaact
     2401 gctattattt gttttctcat tttatgccta ttgctcaatt tatctcactt aaattaataa
     2461 caactgtctt ttcatacaaa acctacaatc aagtttttcc atatattcta catgcatagt
     2521 ctattttttt gatataaaat aacgttcata tttgtaaatt tatttaattt tcaaataagt
     2581 atattgaata ataagcaatt ctattgtatt attgtaattg tagaataaaa ctaaatcggc
     2641 aaaactagag aaatttagtg acgcaaagct atagggacta acgcttataa aataaactat
     2701 gagttatgtc agccagttgc caaaaagaga tattcttttt gtttttatga aagtttttta
     2761 gggggataat aatatatatg tttagagata aaatggatag atgtacacat atgttgactg
     2821 cttatattgg tagttcatat gattattgtg attttataga tacacaacta gatgatttta
     2881 tactagagta cggagaaaat gtggtagaat cttgtttgca tcaagtgatg gtattagtaa
     2941 gtaagtataa ttaaatagat ttggttttct agagaaccct ttttagtttt atctacttta
     3001 aatagcctta tgttagaaag ctaattaaga tagatttcta gtattcataa ttttattttt
     3061 tatttaaaat acatataata aagatgtaaa tttgtatcaa ataataaata tgagaactta
     3121 aattctctta tctatgtgaa aataaacatc tcttaaaatg aaattctgta aacataaatg
     3181 attttttagt ttgaaggatt agattaacaa aattataaat ttgcaaataa gctatattaa
     3241 aaaatataat aattttttat ataacttatt cttttataac ttttaatttt aaaagaggag
     3301 aatgtatata agagtattca atgtactaga aaaaatcaaa agaaatatta gaagaaatgg
     3361 atattgagta atcaaagaaa agattttgat attacaaatg atatgtttca agaaagcatt
     3421 cctatcacaa caaaaattat tcttgaagac atgccttcaa atgatgaatt aaatcatgtt
     3481 ttttcaaaag gttgtgaacg aaaaatgaaa aagagaaaaa ttgtattaat cacactacta
     3541 ttaataggtg ttttgttatt aggaagtatc ctatataatc tttttttagg aaaaacagct
     3601 aatatttcaa tgctaaagga atcttggaat tttgatatac caatacctaa caaagaaatt
     3661 gaagtatttg atactcaaga tagtattaat ggagatggac aaagttattt tattcaagga
     3721 ttttctgaaa aaaatttcaa aaaagttttt aatttaaaag gtggtatagt tgtaagcaaa
     3781 gataatatta atgaaataga aaaatatatt gataaattta aaagagattc tgttaacatt
     3841 aataaaagta ataaaaataa aattgaagaa gattttaaaa aatataagtt agaagttaaa
     3901 aaagatgata agtatatata taagagaaat tatgagaact acgtagtttt aattataaaa
     3961 aaagatgaac aaaaattata cagtttaata tggaatcagt aggtacatat gtattattaa
     4021 gaattataaa taataaaaat aaattttagt caatatacaa aaacctttta aattgtctgg
     4081 aaatcacctc ttcatttgtt ataaaaacat acaacaaatg aagaggtgat tttatggttt
     4141 taaaaaaaat aattataagt ggatatgtgc tttcacatgt ttatcaacat tt
//
LOCUS       DAELNY010000242         4165 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.245, whole genome shotgun sequence.
ACCESSION   DAELNY010000242 DAELNY010000000
VERSION     DAELNY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.245"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            184..1056
                     /locus_tag="KPE55_003455"
     CDS             184..1056
                     /locus_tag="KPE55_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494487.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            1053..2342
                     /locus_tag="KPE55_003456"
     CDS             1053..2342
                     /locus_tag="KPE55_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494488.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLEALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2272..2916
                     /locus_tag="KPE55_003457"
     CDS             2272..2916
                     /locus_tag="KPE55_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494489.1"
                     /translation="MFGQSFQNYLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3183..3647)
                     /locus_tag="KPE55_003458"
     CDS             complement(3183..3647)
                     /locus_tag="KPE55_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0494490.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1595 a          480 c          609 g         1481 t
ORIGIN      
        1 aaacttaaaa aatttcagaa acaaagatac tttaatttaa ttaaaaacag aaaaattaaa
       61 atctttagaa aaaatttaga aatttataaa taatatctta agttttagaa tttattatga
      121 aaatacagta aaaatttata aaaataaaat tctataaaat tgtttctggg ggtgtattta
      181 tatatggaaa tgaaaatagc agtatccaat cttagcaaaa gatttgggaa aaaacaagca
      241 cttgataata taaatcttga aatcgacagt ggtatgtttg gtcttttagg aagaaatgga
      301 gcaggaaaaa ccacatttat gagaatttta gccactttat tatcaaaaac agaaggttca
      361 atacatatgt gtggagttcc tattgagaat actaaggaaa ttagaaatat ggttggttat
      421 ttgccacaag atttttcaat gtatcctaac atgacagtat ataaagcaat ggattattta
      481 gcagttttat cggatatacc actaagaaaa agaagaaaaa ttatagcaaa gttattaagc
      541 aaagtaaacc ttactaatta tcaaaatgtg aaagtaaaat ctttatctgg aggtatgaat
      601 agaaggcttg ggatagcaca agcattaatt cataatccta gagttttaat agtagatgag
      661 ccgacagcag gtcttgaccc agaagaacgt ataagattta gaaatttact atgcgaaatt
      721 gcaaatgata gaatcgttat tttatctaca catattgttg gagatataga agctacatgt
      781 gaaaacgtag ctatattgaa tagtggaaaa gtcatataca atggctcaac agaagcatta
      841 gcaaaactag ctgaagggaa agtatatagt attgaagtag ataaaaaaga tattgaaaat
      901 ataaaaagta gatttatagt tataggaatg ttgactcatg gtggaaaggc tatacttagg
      961 attatatcag atgataaacc ttttgagaca gcagtaaatt gtaatccaac tattgaagat
     1021 ggctatatgc ttataatggg aggagataat atatgaatct atttataaaa gaatgtaaaa
     1081 atatattaaa gagtatgata tattatattt ttattgtagt tttagttttt ttctatattt
     1141 ctcaacttgg aagttcagtt tcaaatgatg taagtaaaaa tattcctaat gatggaaatc
     1201 cattagttgc tccacaaaaa gtagagaata ttgaggaata ttactctgta cacaatcaat
     1261 atccatatgg tcaaaaagaa tctatgaaaa cagaaaaagt cattccaaat gcaactataa
     1321 acctaattag agaatatcaa aaaaacacat atacaacgta tccaacaggg gtagttaagt
     1381 atataaaatt agaccaaaat aaaacgaaga ccatagaaaa atatatagaa gaaataactg
     1441 gtaaaagtat tagcgaaata aattcacttt ggaaaactga tgaaagtata aaaatagatt
     1501 attctaaagt atctcttgaa agatttgaag aaataatgca aagagtagac gacattttag
     1561 gaggagggtc tgcatatgct tttgacaagt tacaaagatt tggagtagtt ccagttacct
     1621 atgatgaagc tatgaagaat tacaactata caatacaaaa agataaagtt agtaatgggt
     1681 atgcaagaat tttttgcgat tatataggta tagttttaag tatatttcca gtatttgtgg
     1741 cagtttttat gtcacttaaa gataagcgtt caagaatgag agagattatt tattcaaaac
     1801 aagtatcttc atgcaaaata gttttatcaa gatattttgc acttgtcttt atgatgatat
     1861 tgcctgtatt aattttaggt atgaaagatt tacttccttt gattttcttt gctaaagata
     1921 aaggtatatc tattgataac ttagcattta taaaatatac attatggtgg atactaccaa
     1981 ctcttatgat agttacagct gtaggaatga gtattactat attaacagat acaccagtag
     2041 caattattgt gcaattaatt tggtggtttt acagtataaa ttctacagga ctcaaaggtg
     2101 attattcaac aatgggacta atgataagac ataatagtgt attaaatgga gatattatta
     2161 tacaaaactt caatcaaatt atgattaata gaattgtgat aagcttggaa gcattggtgc
     2221 ttgttttgtt agctatattc ttatatgagc aaaaaagaag ggggaatctt gatgtttggg
     2281 caaagttttc aaaactattt agttctaata aaagaaaact tcaaattaag cactttaact
     2341 aatttaatat gttcaattat attgctatgt atgattcctc taatacaagg agtagctaat
     2401 ttagacagaa tttcaacagc tatatgttta gaaaattatg ttgcaataat agggatagta
     2461 atgttggtac ctgtattttc gctagagcaa tcaaaagaaa ttgatgagat agtatcttca
     2521 aaagcattat ctcaagttat tgtacaatta attaggatac tgatgtcaat tataggtata
     2581 gtggtattaa tcataggttt tgtaaatgtt ttaatatatc ttggatgtaa ttttccagta
     2641 gttgagtata ttattggaag tttcgcatca gctatgttta taggaagttt aggaatgttt
     2701 ttctcaaatt tatttaataa tacaatcata ggttatatga tatctatagg atatttaatt
     2761 ttaaatatga tgactaaagg caagtatgtt ggaaaatttt ttataatgtc tatgagtcaa
     2821 ggtagttttg atgagaaata ttggctgata cttggaagtg ttgtgttgat aattgcatct
     2881 ctattaataa aaccagtaaa aaggaagatt agataataaa atgagagctt agataaaata
     2941 aaggtaatac aaataaaaag tacatttatg aaatataaat ctatttttat atacacaaac
     3001 ctacttacaa tctctttata tatgtagata gatttatata tccgtagtat aaaatatata
     3061 tgtataccaa attttatcaa aagctctcaa atttatattt caaagacact tattgctatg
     3121 gcctacacat atgtattttt tcattttaat tgttgtatta acaataagac tattgttata
     3181 aactattgat acctcataac aaaaaattct ccatctccta aatgcgtttt ctgtacctta
     3241 gtctcaacta ttttaaatcc ttgagactca taacatttta cagctctcat gttccaggtt
     3301 cttacattta atacaattgg tttagtacta tatttatttc tacattcttg taatcctagg
     3361 ccaattattt ctttaccaat tcccattcca caataattcg gtttcatacc aataccaaag
     3421 aatacagagc ttccttcatc taataagttt ataaaaccta tcaattcttt attgtcattt
     3481 ataaaactta taaaattttt tcttttatct tcttttgcta gtgaaaaatt cttcttttta
     3541 atttcctccc atggaggtag attgtatatt gaatatttac cttcatattt ccaattagta
     3601 acttcaatag catgttcttc agtaaattta actttattta aattcatatg tcctcctatt
     3661 aaaatattta aggtaattaa tggtatttta aagttttaga caatgagtaa aactatttat
     3721 atttatccat aaaatcttta aaaattttac taacaattct tgtaccctgt actttttatt
     3781 ggattcacag aagactataa actaattttg aattaccttc taattcaaaa atatcacgat
     3841 atatatataa tttcttagct tgattaaatt ctgttaaatc cgtttcttat agttgtataa
     3901 ccaacatcaa tatctttatg tgtattagtt aatttattgg ttttaggaac ttattattaa
     3961 tatattgtat ttgctccaat aatccaagta gtaggtaaat tataggcaaa ttatcaaatg
     4021 tatatctatc catgcttaaa ctttagagat acaataacta ctaaattagt attctagtag
     4081 ttacaaaaaa gaatctttaa aaacattaat ttaacttata ttttttaata aattttctaa
     4141 aatttaataa gttaatttaa ttata
//
LOCUS       DAELNY010000243         4148 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.246, whole genome shotgun sequence.
ACCESSION   DAELNY010000243 DAELNY010000000
VERSION     DAELNY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.246"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            248..2347
                     /gene="nrdE"
                     /locus_tag="KPE55_003459"
     CDS             248..2347
                     /gene="nrdE"
                     /locus_tag="KPE55_003459"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG0494491.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLKKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2360..3331
                     /gene="nrdF"
                     /locus_tag="KPE55_003460"
     CDS             2360..3331
                     /gene="nrdF"
                     /locus_tag="KPE55_003460"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG0494492.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3407..4033
                     /locus_tag="KPE55_003461"
     CDS             3407..4033
                     /locus_tag="KPE55_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0494493.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQKLHIGSYDNEPES
                     FDIMNEFCKENKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1668 a          450 c          770 g         1260 t
ORIGIN      
        1 taaataaaaa taatgtcaat tataaagtaa taaaagttta gatataaaaa aatttgtgta
       61 tatactatat gtagtatata atagataaag cactgactat atgtagtgct tttgtttttg
      121 aaaaatactt attgcatttc tataattata gattaatttt gggatttcaa ctgataagta
      181 ccaaaagaaa taataagcaa attaagtgaa attatggcac aagattatgt taaaaggacg
      241 gtaaataatg aattggatag agcttaacaa tcaagtgata attaaaaatg aacaaggcaa
      301 gtaccaattg gaaaaggata aggaagcctt aagtagttac atagaagaat ttatacagcc
      361 aaagttgaaa aaatttaata acctagaaga aagattgaag tatttaatag atgaaggtta
      421 ttattcaaaa gaagtaataa atcagtatag catagatgtt atcaaaaata tatatgatat
      481 gattgataaa cataagtttg aatttcaatc atatatgagt gcaaataagt tttatcaaaa
      541 ttatgcatta aagagcaatg atggaaagga aatattagaa acatataatg ataaagtgtt
      601 aattgtagct ttgacactag gaaatggcaa taaagattta gctttaaact tggcagataa
      661 aatagtaaaa caagaatttc aaccagctac acctactttt ttaaatgctg gaagaaaaag
      721 ggctggagaa atggtatcat gttttttact atctgtagaa gatagtacag aaggtatatc
      781 gtatgctata tcttcatcaa atcacttatc aaagattggt ggaggagttg cactaaattt
      841 atctaaactt agagcatctg gcgaatctat caaagatata gaaggtgcag ctggaggtgt
      901 tgttggagta gctaaaatgt tagaacaatc atttagttac tttaatcaaa tgggagctag
      961 acaaggttct ggtgcggtgt accttactgt attccatcct gattttgaat tgttgatgga
     1021 tactaaaaag ataaatgcag atgaaaaaat aaggcttgct acattatcat taggagctat
     1081 aataccagat aaatttatgg agttggcaga aaaaaatgaa gtagcatatg cattttatcc
     1141 tcatactgtg tataagaaat atggagtttc tcttgatgag atagaaatgg atgagtggta
     1201 tgataaattg gttaatgata gtgatataag aaaaaaagaa ataaatccta ggcagatgtt
     1261 gacaaaaata gcacagatgc aacaagaaag tggatatcca tatgtagtgt acatagatac
     1321 agctaataga gaacatacat taaaagatgt tggaattatt aaaatgtcta acttatgttg
     1381 tgagatattt caatatcaaa caccttcaga aatagagggg tatggaggaa aaaatgaatg
     1441 gggacaagat ataagttgta acctaggttc tttaaacata gccaatgtaa tggataataa
     1501 aactatagaa agtacagttg aaacagcaat aagagcactg tcatttgtag cagatagcac
     1561 agatataaaa ccagtaccaa cggtaagcaa tagtaacagc aagtctcatt ctataggatt
     1621 aggagctatg aatttacatg gatacttagt aagagaaaat attttatata cttcagatga
     1681 tgctatagat ttttctaatg tattttttgc aatggttaga tactattcaa ttaaagcttc
     1741 tatgaaaata gctatagaaa gaaatcaaac ttttgaagga tttgataaat cagaatatgt
     1801 taagggaaga aatagcaagg ttttatcaaa atactatgaa caatcttatc tacctaagtc
     1861 agaaaaggta agagcattat ttgaagggat ttatatacca acaaaagaag actggacaaa
     1921 attattggat gaggtaaaag aaaaagggat atataatgca tatttaatgg cagtagcacc
     1981 aactcaaagc attagttatg ttcaaaatgc tacatcaagt ataatgccaa ttactgagcc
     2041 agtagaagta agaacatatg gagactctac tacaatatat ccaatgccat ttttaacaaa
     2101 tgataatatg ttgtattatc aatcagcata caggatggat atgagaaagg tcatagattt
     2161 agtggcaaca gttcaaaacc atgttgacca aggtatatca acaacactgt ttgttacaga
     2221 tgaaaagaca actagagata tagctagaca ttacatatat gcatataaaa aaggtttgaa
     2281 aagtttatat tacacaagaa caaagatgac aagagatact catgagtgtc tagtgtgttc
     2341 agtataggga gagaaatata tgacatttaa tttacataaa atacataatg cagttaactg
     2401 gaatagagaa gaagatggat ttacacaagc tttttgggaa caaaatgtaa aacaattttg
     2461 gcttcctgaa gaaatatctg tttctaaaga cataaaggta tggaatgaac taagtaataa
     2521 agaaaaggaa ttatataaaa aagtattggg tggtttaaca ttactggata caaaacaggg
     2581 gaataatgga ataccatcta tgatgagttt aacagaaaac ctacaaagaa aagctgtctt
     2641 gtcttttatg gggactatgg aagaaataca tgcaaaaagt tattcttcta tatttatgac
     2701 tctactttct aatttagaaa tagatgaatt atttgaatgg atagaaacag aaccaacatt
     2761 acaaagaaag gctgatttag ttttagctca atacgaaaat actacaaatc aagagggatt
     2821 atacctatca atggttacaa gtgtattttt agagagtttc ttgttttatt caggattctt
     2881 ttatccattg tatctatctg gtcaaggaaa gatggtagct agtggagaaa taatatcgct
     2941 tattcttaga gatgagtctt tacatggtaa gtatatagga ttgctagcac aagaaatata
     3001 cgattcattt gataaaatgg ataaggagat gttagaagag aagatgtatt ctatacttta
     3061 tagccttatg gagaatgaga tagagtatac aaatgttata tacagagaaa gtgggcttga
     3121 aaaagaggtt gtaaactttt taaaatacaa tgcgaataga gctttagaaa atctagggtt
     3181 tgagaagcta tatactgtag atgctataaa tccaattgta ttaaatggtc ttagcactga
     3241 aaccaaaaca catgatttct tttcaactaa gggtaatgga taccaaaaag gagtgtatga
     3301 ggaattagag gatgaagatt ttataatata atttataatt atgacacact gttgtcactt
     3361 ttagcgtgat aatatcaatg tataaaatat atatggaggt atttttatga aatatgaatg
     3421 gagaaaaaga gataaagagt tatatttacc taaaaagaat cctgtaatag ttgaaatacc
     3481 agaacagaaa tttatcatgt taaaagggag tggagaccca aattctaaag aatttactga
     3541 agcaatagga gtactttatt ctttggcata tgcaataaaa accatgccta aaaaaggaat
     3601 tgtaccagaa gggtattttg actatacggt ttttccttta gaaggtatat gggataaggg
     3661 agaaaaaagt aaaggcttag atgtacttat taaagaagat ttaatttaca ctataatgat
     3721 acgtcagcca gattttgtta cacaagacct gttggataaa gctactgata tagtaaagaa
     3781 gaaaaaacct cataatcttc ttgaaaaagt tagatttggg tctattaatg aagggctatg
     3841 tgttcaaaag cttcatatag gttcatatga taatgaacca gaaagctttg acattatgaa
     3901 cgaattttgc aaagaaaata aacttataag aaaatcttac acacatcgtg aaatttatat
     3961 aagtgatgct agaaaagtat cgcctgaatc attgaagacg gttttacgtt ttgaagtaga
     4021 aagggaagta taaatatttt gaataattta taagagttat aaaaagtgaa gtctgttgag
     4081 acttcatttt ttgatgcaat tttaaaatta aatttgatgt ttcataatat tttaaaaact
     4141 ttctactt
//
LOCUS       DAELNY010000244         4047 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.247, whole genome shotgun sequence.
ACCESSION   DAELNY010000244 DAELNY010000000
VERSION     DAELNY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.247"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(541..1860)
                     /locus_tag="KPE55_003462"
     CDS             complement(541..1860)
                     /locus_tag="KPE55_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0494494.1"
                     /translation="MLNINVFWIFLPIFSTTIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1854..1982)
                     /locus_tag="KPE55_003463"
     CDS             complement(1854..1982)
                     /locus_tag="KPE55_003463"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494495.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(2121..2831)
                     /locus_tag="KPE55_003464"
     CDS             complement(2121..2831)
                     /locus_tag="KPE55_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494496.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKNTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT         1521 a          571 c          298 g         1657 t
ORIGIN      
        1 aataattact agttgatttt ttctataaat aattaaatct tctacattaa aaatcatttc
       61 ttcagaatct atcacaacta agtcctcctc aaattagtaa aatatttttc tttaatattt
      121 attactaata tcaactttac tatctaatta gttatttaat tgttgttttt atgttcctag
      181 ttataaagtt tatcaaattt acattttatt tttttatact atatagaact tttggtcaat
      241 aatgttacca tactaaggtc gcatttgcag ataaaaataa ctctattttt ctaaatttat
      301 ttaagatata tcatgcaaat aatataacta taaaacctat caacatttta aattcaaaca
      361 aatataaatc acattttttg ttctattatt ttattgagta ctaaaaagga tgttatctta
      421 ttagcatagc attcaaccat ttttatcacc tccaaatatc ctatgcctta aagatagcat
      481 cttctatttt atctttcaat actactgctc caactgggca atttagagcc ccaactagaa
      541 ttagttccta gttaatggta tataaatagt aattgtaaac ttgtttttat ctgagtatat
      601 ttccacattt ccattatatt tttctactga actttttaca ctacttattc ctattccatg
      661 taaaaaggaa tctttcttac ttgtaataac cttattattt tttaatacaa cttcatttat
      721 ctttgtattt tcacatttta ttacaaataa cctatttata acagttcctc ttaatttaat
      781 ctttttttgt atacttgtgt ccttaatctt aatacaagct tctattgcat tatctattat
      841 atttgaaaat atactacata tatctggcat ctctataaaa ttacattcta aaaaattaat
      901 atctactaga aaatatatat tattcatatc acaaatagat tttttatcac ttaaaataac
      961 atctagaatc atattctgag tattaaatat agtattacat tcttttattt gcttatttat
     1021 atctttaata tacttatctg caaactcatt tttcccataa acattctgaa tacaaatgat
     1081 atgattattc atatcatgat atagttttct tattttcaac tgatattcct gtaaatttaa
     1141 ataatattta tattgcatat tcattctttt ttttgtaatt tcatattcta tctttaaact
     1201 attatctttt attattcttc ctattatgct tactaatgaa agatttgata gtaaaaatac
     1261 agttgatatt gtaagtatta ttaaactctc tttagaatcg ataatcttat ctttaaaaat
     1321 aaattcaaat attactatta caccaataat gtttgctatt aatggaatag ataaatatat
     1381 acaatccctc ttgtttattt ttgtttttaa tctgataaac ttaagcaatg gtattaatac
     1441 tattagtaat gatttagata tcattattaa ctctaacttc aacaaattat cctctaataa
     1501 atcattcata ttttttatag aattaagtgt cattactata cttaatccaa tactatcaaa
     1561 tccaattaat aacatccaat atagcaaact tataaataca cttttccatt tatccactac
     1621 ataatttatt ttatacaata catatgtaat aaatatacat ataaataatt taatatttat
     1681 attaaactca attgtattca acatagtaat tatcataata gcaattacta tgctactatt
     1741 tagggttaat ctattctttc ttaactcact aaattcattt aataaaaatt ttaatacaat
     1801 ccattctatc gtcgtagaaa atataggtaa aaaaatccaa aatacattta tatttaacat
     1861 aatacatctc ccaatacata tgttaatttt ttctttaaat tagccattct atatttacta
     1921 actggtatat ctttaccatt aataactaca gtatttttac atataaattg aatatactcc
     1981 aaattaatta aatagctctt gtaacatttt taaaaattta ttctttctta aatcactgaa
     2041 ttcatttgat aaaaatttta atacaattca ttctatcgcc gtagaaaata taggtaaaaa
     2101 aatccaaaat acatttatat ctaacataat acatctccca atacatatgt taattttttc
     2161 tttaaatcag ccattctata tttactaact ggtacatctt catcattaat aaccacagta
     2221 tttttacata taaattgaat atactccaaa ttaattaaat agctcttatg acatctaaaa
     2281 aaattatatt tttctagctc taattctatt ttatccatac tatttttagt ataataagta
     2341 tcatttttag tatgtatagt caaaaccttt ttcctaacct ctatatacat aatactatta
     2401 attaaaattt tattgattat acctttattt tctatcagca taaaattttc tctttttttt
     2461 ataatatccg atatacaact caaaatactt tcatttaaat ctccaaaatc tataggcttt
     2521 aataaatatc tataagccct taccttatag ccttcctgta tgtaatcaac tattgaagtt
     2581 ataaatatta tttcagaagt atcattagtt tctctaatta ttttagccac atccatgcct
     2641 gttagttgaa gcattttaat atctaaaaaa aatatatcaa taccttctaa atttgtttcc
     2701 aataattctt ctcctgaatt aaactcaaat agttcaattt gactagaaat accctctaag
     2761 gactttaata tacactctct taaaaaagct atttgtgtaa tatcatcctc acagataact
     2821 attctataca taaacacccc tccttagaca ttagatctga aaacaaataa aatatcaatt
     2881 tataaataat tatttagtta tacaactaac atggtcatca aatactatta ttcacttaaa
     2941 aaatcacccc ctatttttag tatgttaaca acaaaataag aagatatctt agatttttaa
     3001 aaacaattaa tacttaatgt agttttgtat actatttata ggtaattttt tatatcattt
     3061 taaacattaa aattattgat gtttaagaac tattatattg atagtaatat ataattaatg
     3121 atatcaaaaa tttttaatta gacttctctc ttattcaaga tttttctata taaaatctaa
     3181 gaataaatag aaaactaaca tctaaccttt tattttttat tttcgatcta tttacttgaa
     3241 ttttaacata aatctatctt ttagtatata ttaatcatgc tgaatttgtg atgacatgta
     3301 tacaaaatat cgtttagtat tttctaataa aaaatattac tgaaattaaa aatctacaat
     3361 agcatttaac aagtaaaaac atgtttacac aaatatataa tttaaggtct ttaaaataga
     3421 gtatgtatta ttctaaaaat tagttatagt tatcacataa aaatagaaaa gaattttcaa
     3481 aagatttaaa gaacatacat acttcaattg cagaagatgt aatgttaagt gaactagcat
     3541 tcttcgagga aaaatagact caaaaaatta gaaaatagaa tcaggtttta gatgatttaa
     3601 gtatttattt tcaaaaaaga aatttgattc taagttaata aagaaatatt taaaaaaatc
     3661 aaaaaatttt cttaaataag taataggttt tgtaaaaatg gaaaaatatc aaaataagac
     3721 tatgattttt aaaactatag ccttatcaat aaatttttat ttaaagaaca cacataaata
     3781 tttttaaacc taatttatgt gaagttttat gtatttttta tataagtagt attctaatct
     3841 tactcatcta atataccaaa aagactgtaa gtaagttcac ttttatgcaa acttacccac
     3901 aatctctttt acacattaat tctatttatt aaaattaaaa atatttactg ctattctacc
     3961 caataaagat gatactgttg ttgttctggc tggtactgtt acttctattt ctcctgtatc
     4021 tgctgtttta ccacttgatg tgtgtgt
//
LOCUS       DAELNY010000245         3962 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.248, whole genome shotgun sequence.
ACCESSION   DAELNY010000245 DAELNY010000000
VERSION     DAELNY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.248"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(122..1414)
                     /gene="brnQ"
                     /locus_tag="KPE55_003465"
     CDS             complement(122..1414)
                     /gene="brnQ"
                     /locus_tag="KPE55_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0494497.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
     gene            complement(2533..3825)
                     /gene="brnQ"
                     /locus_tag="KPE55_003466"
     CDS             complement(2533..3825)
                     /gene="brnQ"
                     /locus_tag="KPE55_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0494498.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         1372 a          669 c          443 g         1478 t
ORIGIN      
        1 ttatcaaata ggattaataa tgttctttta tctacaataa tttaataatt cttttaaata
       61 taaaaaggaa ttgcctatat aatttaaagg caattccata attatcaatt agtttttatt
      121 attatgcagt tttactgctt ttagatggaa taaagtttcc tattattcct gctattacaa
      181 ctggaactat ccagttaaat cctaatgaag caaatggtaa gctattaact agaggaattt
      241 ttataccaaa acttgttgca actgttaata cacttactaa taatgccatg tatgcagcac
      301 atttgaacac gttgtcattt ttaattttat tactaaataa tgctagtata actagagtta
      361 tagttgctgg atataccata cttagtatag gagaagagaa ttgtattatt gtacttactc
      421 caaagtttga tatgattgca ctaaatacac aaactactat aactatatgc tcatacttta
      481 attttccatc tgtaagtgtt gagaaatatt gaccagctgc tgaagaaaga cctatagctg
      541 ttgttaaaca tgctaatgca actataatag ccaataaaat tttacctgta gttccaagta
      601 aagctgctgt tattgcaact attaaagctg tttgttgaac atcttgtcca tattgttgag
      661 acacagttgc tccaagataa cataatccac cataaactaa tgccattcct attactgcaa
      721 ttacacctgc acttattgtg gctttcatct tttctttctc acctgtataa cctttagcta
      781 ttactgacat gattataatt gatgcaaata cactagctgc caatgcatcc attgtttgat
      841 aaccttgagt tataccttct gcaaatacat tttgtattaa agaactgtct cttatttctc
      901 ctaatggaga tacgatacct ttaactatta atactgctaa agctataagt aaagctggag
      961 ttaatattga cccaactata tctacaactt ttgatggtct tattgttaaa accaatacta
     1021 gagcaaagaa tactatagag aatattactg ggcttattcc agaacctatt gtaggtaata
     1081 tacccatttc ataagttgtt gctgctgttc taggtattgc taaaagtggc cctatacaga
     1141 ttaccattgc acatcctaat gcaatactta atcctttgcc tgctcttgag aacatcttcg
     1201 atgtgtctcc attgaactta gttgctgcta taactgctaa taatgccaaa ccaccatctg
     1261 caaaaaggaa tcctatgaaa gctactagcc aactacttcc agataaaact cctagatatg
     1321 gagggaagat taagttccct gcaccaaaaa acattgcaaa tagtgcaaat ccaattacaa
     1381 ttacatcttt aaaattgatt tgtttctcac ccatttaaag cacccccatt tgaattttac
     1441 taccttttac taaaaaataa gtccaatctc ttaattaaat gaaaaccttt tgacgagatt
     1501 ttatttaatt atacattttt gacttaaatt tgtcaattat gtttcataat ttataaaatc
     1561 ttcacaatat ttaatacaac tttcttaatt aaacaaaatc aacctgtata ctttaaacat
     1621 agttggtttt ttgttttttc tctaatttct cttgataaaa ttaactattc cgaataatta
     1681 aaatattaag attttttaga ttattcgatt tattattaag ttgtattgct ttatatactg
     1741 tatatatcac tctccttttt tacattcata taatcttttt aattcaattt aaattttaaa
     1801 tattttgtgt ttattatata atctgaaaaa aaagcagtca atagcatttt taattttttt
     1861 aattttcaaa attatgattc tcaaaaaatc gttttccttt tatcataaca cctatttacg
     1921 ttaattcttg tagtattttg tatttatttt gctaaattga cagttaactt tattcaattt
     1981 ttattctact tgcgtagatt tacctcttgt tttttactct taaaaccatt ttttctgaaa
     2041 attccttatt atgatttatc aacttatttt tcttcataac aattactctc taacttccaa
     2101 cttacttaaa ctttgcagtt gctagtattc acgtttacat acttatatgt cataattaac
     2161 taaaaattct taatatatct ctcaatgaaa acaatttact catttattta atacatttat
     2221 ataatcatat gtcttaaatt ttgttttatt attttattat tgtcacaatt ttatttatat
     2281 tatatttttg taaaaatcat ttaatatgta tacttaaatt tataaaatta ctctaaaatt
     2341 cttttttaat ctctattatt gttaaaaaat aaactatata tttttttgat attttcttat
     2401 actttttcca tattaaatac aaataattaa atatagaatc taaaaataaa aggagttatt
     2461 tgtacaaata actcctttta cttttagtta actcatttat ttacaattga ttatatttta
     2521 attaaacaaa acttagtttg ttccaagtgt attagattga ctctttgatg gtataaaatt
     2581 accaattatt ccagctatta taactggcac cacccagtta aatcctaatg aattaagagg
     2641 taaagaattt actactggaa cagctactcc ataactagat ataactgtta gtatacttac
     2701 aagcaatgac atatatactg ctcctttgta tacattatca ttcttaattt tttcattgaa
     2761 gaaagctaaa attattaaaa caattgatgg tggataaact atacttagta taggagatgc
     2821 tatctttatt atagttccaa caccaaaaga tgctactact gcactgaata cagatacagc
     2881 taatactatt ttttcataac tcaatttacc ttttgtaagt ctagagaaat attgacctgt
     2941 agcagatgta agacctatag atgtagttaa acatgctaat gatactacta ctgctaaaat
     3001 cgctttccca acatttccaa gcaatccttc agttatattt actattactt gtgattgtac
     3061 tgcatctgtt ccatacattg ttgatacagt tgccccaagg aaacataatc ctccataaac
     3121 tagagctaat cctccacaag caagaacacc cgccttacca atcattttca acttgtcact
     3181 aatagtagaa tatcctttat catttaaaga tactattagc actgatgcaa gtgctattgc
     3241 tgcaaaagca tccattgtct gatatccttg acctatacct tctgcaaata cattaggtat
     3301 cataggttta tctactatat ctccaagagg tgatataata ccttttataa ttataaatgc
     3361 taaagctatt agaagagctg gagttaaaaa ctgtccaact atatctacga ccttagaagg
     3421 tcttattgtt agtacatatg aaatcgcaaa aaaaagtata cagaataata acactggaat
     3481 tgaagttcca aacaaaggca ttatacccat ttcataagtt gttgcacctg ttcttggtat
     3541 agctaagaat gggcctatac ataaaatatc tgcacaacct ataagaatcc ctaacttcat
     3601 accacatctt ttaaacattt caactacgtt tccatcgaat ttagctgtag ccattactgc
     3661 tagaagtgcc aatccagcat cagcaaacgt aaatcctaag aaggcaatta accattctgg
     3721 acctgtaatt attcccaagt aaggtggaaa tattaagtta cctgctccaa agaacattgc
     3781 aaataacgca aatccaaata ctataacatc cttggtctta cccattataa aatccccctt
     3841 tttaacctta ttaaattaat aaaatgtctc gattgtttac gcttgcataa gtaagttgaa
     3901 ttaaacaata agaacttatt attaaattaa taaaattaaa taaattaaat ttttaatata
     3961 tt
//
LOCUS       DAELNY010000246         3953 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.249, whole genome shotgun sequence.
ACCESSION   DAELNY010000246 DAELNY010000000
VERSION     DAELNY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.249"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            45..248
                     /locus_tag="KPE55_003467"
     CDS             45..248
                     /locus_tag="KPE55_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0494499.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
     gene            412..1566
                     /locus_tag="KPE55_003468"
     CDS             412..1566
                     /locus_tag="KPE55_003468"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG0494500.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1579..2583
                     /gene="rseP"
                     /locus_tag="KPE55_003469"
     CDS             1579..2583
                     /gene="rseP"
                     /locus_tag="KPE55_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG0494501.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2765..3820
                     /gene="ispG"
                     /locus_tag="KPE55_003470"
                     /gene_synonym="gcpE"
     CDS             2765..3820
                     /gene="ispG"
                     /locus_tag="KPE55_003470"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG0494502.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
BASE COUNT         1535 a          394 c          775 g         1249 t
ORIGIN      
        1 aaaatagata aaatgtgata gaatatattt tgcgaggtga taacatgatt tcatacgctc
       61 cattatggaa attgttaatt gacagaaaaa taaagaaaat ggaatttgta aatatatcag
      121 gtataagtat ttctgtttta ggcagactag gaaatgataa atctgtttct atggatacaa
      181 tggagaagat atgtttagca ctagactgta aaatagaaga tgttgtggaa ataaaaaaga
      241 gcatttaata atactagaat attttataat attccacaaa atacaaaaaa taatagatta
      301 attttaatct gtggaataat atatgaaatt tctatgaata gtaaaaataa ttgttttagt
      361 agtgttataa tgttttgaca gggtatattt attattagaa aggattatta tatgaaaaag
      421 atatcaattt taggttctac aggctctata ggtaaacaaa ctttggatgt agttagagat
      481 aatagagata agtttgagat agttgcaata tcagctaaca gtaatattga attattgcta
      541 gaacagatag tagagtttaa acctaaatat gtaacagttt ttgaggaaaa taaagcattg
      601 aaattaaaag aaatgttgcc taaaaatata gaaatagaag ttttagcagg aatggaagga
      661 ttaaaaataa tatcatcact tgatgaagtg gatgtccttt taactgctgt agtaggtatg
      721 ataggattag tcccaactct ttgtgctata aagaagggaa tagacatagc attagcgaat
      781 aaagaaacgt tggtaactgc tggtgaactt gtaatgaaag aagcagaaaa atacaatgta
      841 aatattcttc ctgttgatag tgaacatagt gctatatttc aatgtctaaa tggagaaaat
      901 aaaaaaaata tagaaaagat aatacttaca gcatctggtg gtccatttag aggaaagaaa
      961 aagggagaac ttgcaaatat aactaaaaat gaagctttga agcatccaaa ttggagtatg
     1021 ggaagaaaaa taagtattga ttcttcaacg cttatgaata aaggacttga agtgatagaa
     1081 gctagatggt tatttggagt agaacaagaa aatatagatg tagtagttca cccacaaagc
     1141 ataattcatt caatggtgca gtatacagat agctcaataa ttgctcaatt aggatgtcca
     1201 gatatgagac taccaataca gtatgctctt acatatccag atagaatgga aagtagcttt
     1261 gaaagaatga atttttcaaa atttagtact ttaacttttg aagaaccaga tttagaaact
     1321 tttccatgtt taaaattggc atatgagtgt ttaaaaatgg gaggaactta ttcctctgtt
     1381 ttaaattcag caaatgaagt tttagtaagt gaatttttag aggataaaat tggtttctat
     1441 gacataccat attacataga gaagacttta gaagttcata gtagcataag tgaaccaaca
     1501 ttagaagaaa tcttagaaac agatagatgg agtagagctt atgttgcaaa tctgataaaa
     1561 aaataggaag gtgaatttat gactataata gccgcattaa tactatttag tataattgta
     1621 ttaatacatg agttaggaca ttttatattt gcaaaaagaa gtggtatcaa agtaaatgag
     1681 ttttctatag gtatggggcc taagatatat agtgtaaaaa aagatacaga gtattctata
     1741 agagcacttc caattggtgg atatgtcagc atggaaggtg aagatgaaga acaaataagt
     1801 ccaaattctt ttggaaacaa gtctatatta caaagattta gtactattgt agcaggacca
     1861 atattcaata taatattagc agcaatactt ttagttccag tatttttgta tataggttct
     1921 ccaacgacta aattaggaaa gataatgcca gatactcctg cacaggctgt agggcttcaa
     1981 gtaggagata agataaataa aattaatggt aattctgtta agacttggga tgaagttgca
     2041 aatataataa atacttcttc tggtggagaa ttaaaactta gtataactag agatggaagt
     2101 gataaagttg taaatgtaac acctaaaaat aacaatggaa agtatgaaat agggattcaa
     2161 ccacaaagag aaaaagactt tttggggtca atagtaaatg catgtaaaac tactgtagat
     2221 atgacaaaac agatgctaac atttctaggt caaatgatta caggacgtgt accaggaggg
     2281 attggcaatg ctgtagcagg accagtaggt gttattggta tggtatcaga tgctgctcgt
     2341 acaggcatca taaatgtagt gtatttagca gctgtcataa gcttaaattt aggtatagta
     2401 aatctattac caataccagc acttgatgga tggagaatac ttatgctgtt actagaagct
     2461 gttagaggtg gtaaaaaact cgacccaaat aaagaaggca tgataaatgt tgttggtttt
     2521 ggagctttaa tgttatttat gctttttatc acatataaag atatactaag attatttcaa
     2581 tgatttagtt tttagagatt ttaagaaaat aggctatgta gttttaaata agaataaatt
     2641 aatttttata tatgaaattt attcttattt aactagatag ctttttaatt tataatatac
     2701 atgtaggtta taatatttta atatttaaag agattcaata taaaataata tatagaggtg
     2761 gaatatgagt tacgagagaa gaaagacgag agaagtaagt gtaggaactg taaaaatagg
     2821 aggagaaaat cctataagta tacaatctat gacaaatact gatacaagag atgcagatgc
     2881 tacaatagct caaataaaga gattagagga agctggttgt gatatagtta gagttgctgt
     2941 tcctgatatt aaagctgcta aaaatatagc aaagataaag tctagtgtaa atataccaat
     3001 tattgcagat atacatttcg attataagct agcccttgaa gctatagaac aaggtgtgga
     3061 tggaataaga ataaatcctg gtaatattgg aagtatagag agagttaaga tggtagttga
     3121 gaaatgcaag gaaagaaatt taaaaattag aattggagtt aatggaggtt ctttagagaa
     3181 agaactttta aaaaagtatg gctctgcaac tgctgaagca ttagttgaaa gtgcaatggg
     3241 acatattaag attcttgaag acttagattt tcataatata gttatatctc taaaatcatc
     3301 cgatatttat aaaactgttg atgcatatga attaatatca aaaaaagttg attatcctct
     3361 tcatattgga attacagaat ctggaagtgt tcacaaagga acaataaagt cttctatagg
     3421 ggtaggagca cttcttctta aaggaattgg agatactgtt agaatatctt taacaggcga
     3481 ccctgttgaa gaggttattg ttggtaaaca gattttaaga agtttgggac ttttaaatga
     3541 taagattaaa gttatatcat gccctacatg tggtagatgt aatatagact taattagtgt
     3601 tgtaaatgag gttgaagaaa agattggtag tatggaaaaa aatatcactg ttgcaattat
     3661 gggctgtgct gtaaatggac ctggtgaagc cagagaggca gatataggta ttgctggggg
     3721 taaaggtgaa ggtcttttat ttaaaaaggg tgaaatagtt agaatgattg atggtaacaa
     3781 attagttgat gagttgctag aagagattga aaaattatag tatacaattc atatatatat
     3841 taattgacta gatttttaaa tacttttgta ataagttttt atttacagta tttaattatc
     3901 tagtcatttt ttattttata aacttcatta aatgtaagaa acttatattc taa
//
LOCUS       DAELNY010000247         3943 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.250, whole genome shotgun sequence.
ACCESSION   DAELNY010000247 DAELNY010000000
VERSION     DAELNY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.250"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..981)
                     /locus_tag="KPE55_003471"
     CDS             complement(64..981)
                     /locus_tag="KPE55_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG0494503.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFRNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1243..1620
                     /locus_tag="KPE55_003472"
     CDS             1243..1620
                     /locus_tag="KPE55_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0494504.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1776..2225)
                     /locus_tag="KPE55_003473"
     CDS             complement(1776..2225)
                     /locus_tag="KPE55_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0494505.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2389..2793)
                     /locus_tag="KPE55_003474"
     CDS             complement(2389..2793)
                     /locus_tag="KPE55_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG0494506.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2906..3772)
                     /locus_tag="KPE55_003475"
     CDS             complement(2906..3772)
                     /locus_tag="KPE55_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0494507.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1283 a          676 c          434 g         1550 t
ORIGIN      
        1 tttatttttt cttaaaaata aaataattgc ataatttatt tcaaattatg caatttataa
       61 aatttattcc caaaactctt ttactgcttc gattatctca ctataaggca ataaagtcgc
      121 ttcgattatt gatttatttg tcttttctaa ataatactta atcatataat atccacaagc
      181 atatcctgca caataaggca accctactgg aaaatatcct tgcattttag ctatatcatc
      241 accataaaga taagatgtta tatttcgaaa tccagtttct tttaaagcac tttttattat
      301 tggctttata taagtattta atgtttcgat atctgttcta ctgacccaag gtcctaacat
      361 ttcctctcca aacatccatg ttgcaaaatt ttctgcaaga ccttcattta tcatcatttc
      421 ttctaatgtt atattattat tccactctat aaactgaaat ctaatattgt gattacattc
      481 atgtgctatt aatactggta atctattgat agtatattca ttaggaacca atgatagaaa
      541 tatatatcca ggaatcccac catcacccca gtatccatca cttaatattg tataaggact
      601 atttggattc gccaataata ttgaatattt atagtcctta atgtttaaat catacccttc
      661 ttttataaaa gcatttatag agttttctat ggtttctttg caagtgcccc aaattttatc
      721 agcagataac acatttatat tctttttttg cttttcatca atatccttag gcgataaaac
      781 ccctaacatt tcacttgcta taattacatc atatccacct ttctgtctag caactattgg
      841 aacattcata cacttccact taaattcaaa aggcttcatc aattcatatc tgtatatatc
      901 ttcttttttg tttattggtg catccattat cttcttgtat attttatctg agcgaatttc
      961 tataatattc atatttacca tctcctttaa gacaaagtat atacaattac gtagcgtcaa
     1021 tgtcaatata cattttttat aatgtcacct taatcttttt attatatttt atcctgtaaa
     1081 ccttccgtac atataacatt tttttaaatt aataaatttg tataatataa taataaaaaa
     1141 ttatatactt caaggtttcc ctatgataca atttactaaa tcataattta gattatcagg
     1201 tgcaactata aattattcta tatcaaggag gatatattgg tcatgctaac tccatattta
     1261 atatttaatg gtacttgtga aaaagcattt aatttttatg ctgaggcttt cggaggagga
     1321 aaaactatat ttgcgcgatt agacagcaat ccaaacaatc ctattatgca cgcaagtgtt
     1381 actttcacaa aatatgaagg ttgtataatg ggtgcggata cagacaagcc tgttgtaatt
     1441 tctggcatgg cgatttgtgt tgttctacca tctcgagaag cgatagaaga aatatctgta
     1501 aaacttgccg aaggtggtac acttgtacaa gaatttttac cacacccacc accacatcaa
     1561 aatgatggcg ctgctgaagt acttgatagg tatgggtata cttggtattt aagtacatag
     1621 ttaaaataaa aaaactacat ctaataatca taatatagaa cagataaatt tcaatttaat
     1681 agtgtagtaa aaaagtcgtg tcctaagact gtcacgactt ttatttgtat aaaaatttaa
     1741 gtaaatattt ttgcgaataa atgagaagta taactttaaa ttaatttcat atgtagtatt
     1801 ggaaatgggt taccttgtcc atctatttca gacctatcaa acactttaaa tcctaagtga
     1861 atataaaatc ctacagcttt tttattctgc tcatttacat caacatattt tacattgtat
     1921 ttatctatag catattttat taatttttta ccaataccat ttcctctata tttatcttct
     1981 ataaagagca tctcaatttt atctttatct attcctataa agccctttat tatatcatta
     2041 tcttttatac aaactatatt ttctacttct ttaagtccaa tctcaactaa tggtatcaaa
     2101 gattcaatat ctttttctgt taaaaaatca tgcgttgctc ttactgatga ttcccagact
     2161 tgcaacattt cgtcatattc tgttgaattt atttgtttca tttcaagaat tattccagat
     2221 ttcataaaaa tttcctccct atatcacttt taattgcata acactttttt atacaaaact
     2281 tagaaaggac tacgaaggtt aaatatcata gtcctcatta agtttaaata ttatctatta
     2341 tatattatac tataatctag caaattcact acaataattc acatcagctt atactaaagg
     2401 tgcttctatt tgagtagtaa aaaggaattt atgacaatga ccatcattaa cagtagtttc
     2461 tccacatatt aagtgaatgt gtttatcagt tccaggtatt ctaattgctg gccctgttgt
     2521 aacacatatc ttatggaaat ggtcaaggaa atctacatta tcatttattt tatgaacatg
     2581 atttgtacct ccatttattg gtatagcttc accagtaacc cctgctgcac ggtggttatg
     2641 tctatcctca cattcttctg ctaacttaac actttctgaa tattcatgaa catgtgtcat
     2701 ttcctcataa tcattatagc acttgttgca cttattgtat ttataatcat catacacata
     2761 atctctttca catttataat tttgataact catttttata tctctcccta tattatatta
     2821 agaaattctt gtaaagaatc tctaatgtat aatatgaatt aaagtcaaaa aagttcatat
     2881 tttgtaaaat tatgtattaa tttaattatt tttcccagta tttagggcgt attagtgaag
     2941 ctggtaactt cgtactagaa tctccttttg cagtatttac ttgtaattgt gttaaaaata
     3001 tactattttc aagattagca cctctaatat cacaatctct taagtctgct cctattaaat
     3061 ttgccccact caaatctgta tttcttaaat ttgctgctat tagaaatcta cctcttaaat
     3121 ctgctcctat tagatttatt tttctaaggt ctgctcctat taaatctata tttttactta
     3181 attttttcat gtttttaaga gtagaagtct tgataaatga tttaaccttt cccatcacag
     3241 actcactcgc ttgtgataat aataaattaa cctttgttct atgtgatatg atatccaaat
     3301 taattatctg ttctgggccc atattagtta tcttttcagt ttcttcaatt attaaatcaa
     3361 tttttgattt gattgtatct gatatattta atacagatgc ttctttaaga taccataaca
     3421 tttcatgtaa ttgtctcata attaaaaaca cttcagacat ttgatttgtt agttctggtg
     3481 attgcatcca gtctatcccc ttatatgtta cttgtgctac tttttgacct gaaccaaaac
     3541 aatcatatgc agtacaccct ttaaaacctc gcttcataag actattatgt acagaacatt
     3601 taaaatctgg ttgtaaatta atacaaggtt ttcctgattc cttatcgata ggaaatccat
     3661 ctgaagcaga aaaataaaga gctacacagc aaagtccaaa gcacttctta cagtcaatct
     3721 ttagctcctc aaataactta ttatcatata tatttttatg tatatctatc atattttttc
     3781 tcctcttaaa ctttattttt aatattatat tatctataac taaatgataa tcaagtccct
     3841 tatttaacag ttcttattct taattataat ttaaggtatt atttattcca atagcataca
     3901 taagtttctt tatacttgta acaatacctt tatactaaga ctt
//
LOCUS       DAELNY010000248         3902 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.251, whole genome shotgun sequence.
ACCESSION   DAELNY010000248 DAELNY010000000
VERSION     DAELNY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.251"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(92..868)
                     /locus_tag="KPE55_003476"
     CDS             complement(92..868)
                     /locus_tag="KPE55_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0494508.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(925..1896)
                     /gene="asrC"
                     /locus_tag="KPE55_003477"
     CDS             complement(925..1896)
                     /gene="asrC"
                     /locus_tag="KPE55_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG0494509.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(1909..2700)
                     /gene="asrB"
                     /locus_tag="KPE55_003478"
     CDS             complement(1909..2700)
                     /gene="asrB"
                     /locus_tag="KPE55_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG0494510.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(2701..3708)
                     /gene="asrA"
                     /locus_tag="KPE55_003479"
     CDS             complement(2701..3708)
                     /gene="asrA"
                     /locus_tag="KPE55_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG0494511.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         1238 a          772 c          360 g         1532 t
ORIGIN      
        1 gtttcaaata ttttttcaaa aatataaaaa taactctctc aacaatacta aaaatgtatt
       61 tatcaagaga gttataattt atacaataaa tttaattaag cttttcttta cctaatatat
      121 aagttccaag acccataaat aaagctcctc caacaatatt tccaagtgtt acagctacta
      181 aattataaat agctcctcca atagtaactg tacttattgt tggtgaaaat aatgaaacac
      241 tataaattgt catatttgct acactatgct caaaacctga tgttataaaa gcaaataaac
      301 ataagaatat cataattatt tttgcagtat cactattagt tctaaacgaa cacaacactg
      361 atacacatac tagaatatta cataaaatcc ctctaaagaa aagagctgta aatggcatgg
      421 atgctttact agctgctgta tttgcaaaaa attcagctac aggtcctttg tctactagac
      481 cagttcctac aaatataatg cctaaaacca aagctccgat taaattacct acccaactgt
      541 atgcccatat tttagaagta tcttttatac ttacaccttt atttaacata ccagcagaca
      601 taaccatatt gtttccagta aataactctg ttcccgtcat tataaccaaa cttagtgcta
      661 ttgcaaatga taaccccatt actatcttag tcataggtga acctgcatca gtcaaaagtc
      721 ctccaatagt aaatatcaaa agtataccta tacctacata aagtcctgca aacgcagaag
      781 ataccaaata ttttacttta cttgtgttta ataaatttat cttatttata gctgcattag
      841 ttaatttatc caaagtttct ttatgcataa ctccctcttc cttctattaa attttaattt
      901 attcaaatat atgttttact caatttatag atatttaatt ccactccagt atatgttttc
      961 gtacatttta gttatttctg gaagctctac accttcaagt gcccattttt taaattccat
     1021 aaatcctgtt ctatcaatta tatatcctat atgttctttt cctcctggtg catctttatc
     1081 tatatacttt tctacatatc tataagtatt aagtattatc tttattatgc tgtcctcatc
     1141 tacccataaa agaaaatctt ctgctagtct agggtttttc tttcctgttc tacccattat
     1201 agctaatcta tagtattttt tctcatctct agtccatgca tttgttgggc aatttagtac
     1261 acactctcca cagcctatac acttatcttt atctcttacc accttattat tttctgtttt
     1321 taatgctcca acagatatct tagaacattt ttttacacat gctccacaat ttacacatct
     1381 atccttttca tatattggta aagccatacc aataattcca aagtcattca ttctagcttt
     1441 tatacaatca tttggacaac cagttaaagc cactttaaaa tgtaaatcat ttggaaatat
     1501 agctttttct attcttttag caaattcagt tgtattatat tgagcctttg gacagacttt
     1561 atttcctata caagctgcta tatttcttgt acctgctgct ggataacctg cgtttttctc
     1621 actttgattt atattcattt tttcaataac aggttgtata attttattta tttcttccat
     1681 atcttccata tttattccta atatttcaaa cccctgtctt gtagttatgt gcacctgtcc
     1741 attgccgtac tcacttgcaa ttttagaaac tacagctaaa ctctcagggt caataagacc
     1801 tccaggtact ctaactctta aagaagtctc atattttgtt tttgtaattc tataagcatt
     1861 tttcattact tttttggtat ttaaatctct tatcacaaac ttcaccccct aatcaataag
     1921 atttttagca tatgaataat caaatactgg tccatctaaa catatgtaag tatcatccat
     1981 tttacaatgt ccacatttac ctattccaca acacattttt ctttcataag aaacccaaat
     2041 gttcttttca tctaaatctc tttttaaaaa ttcttctact gtaaatttca tcatcattgg
     2101 aggtccaact acaactatag aagtattgtt tatatctttc atatccaatt ctggtatgta
     2161 ttttgttact aatcctacat taccactata tccttcctct gctccatcta cagtaactaa
     2221 aatatcaagt tttttactcc atctctttaa atcatctgca aataaaatat cttttggtga
     2281 tttaaatcct actattaatt taaacttttc gcatttttct ttattattat aaaaatattc
     2341 aactattcct cttacagggg ctaatgcact tcctcctgcc actactacaa tttctcttcc
     2401 ttcataaagg gatacatcaa atccatttcc ataaggacct cttataaaaa aagaatctcc
     2461 ttctttataa ttaaacaact cgctagttac ttttcctaca tttcttattg taaagtcaat
     2521 ataatcctct ccatatcctg atactgatat tggactttca ccgtatttag gtatagatat
     2581 ctcataaaac tttccaggca atacaccttt tgtattgcag tttactctaa aagtccattc
     2641 tttatcagta tgtttaacta tatctagtat ttttgcactt actggtatat atgggttcat
     2701 ttatacttcc tccttactaa ttactaaatc atttattttt tctatacact ttgaaaatga
     2761 tatgtattgc ggacaggcat catcacatct tccacaacca acacacatat gatatccgaa
     2821 tcttttttta aaatcatgta ttttatgcat agttttaaat ctcattcttt caccttgttt
     2881 ttttctaaaa gaatttccac ctgctatatc tgtatatcca tctacttgac aagaagccca
     2941 aacccttctt ctctcaccta cattttcgtt ttccttatag aaaatatcct gcattgtaaa
     3001 acatgaacat gttggacata caaaattaca tctaccacat cctatacatc ttgtatcata
     3061 ttcatcccat attgaatttc ctatcatttc atttgaatca atattctctg gcacatctac
     3121 agatattaaa ttatcagtta cataatcaac attaaattca gttgcttctc cattaaatac
     3181 ttcaaattca ctatccttta tatctaagta cagtgtatct ccttctatat ttaatccaat
     3241 tgaataatta tcagttttgt tagaatccat gctcacacaa aaacagtttc taaaactttc
     3301 cttacaccct acaagtgcaa actttacttt atctctgata tttttataaa aataatcttt
     3361 ttcttcacca ttatttagat atatttcatc aagtcttttt ataccattta tgtcacaagc
     3421 tcttaagaaa attaaaattt tcttgttgtc taaatcgctt tccttaaact ctttttcagt
     3481 aaaataaaac aatacttgat ttatgggaag tacaacttct tttggtgaaa agtttgcttt
     3541 cttagcaaat tccatttctt caaaagcgtt tacctccttg tatcttataa catcagtatc
     3601 tgaaaaagta cctttaaatg ggattaaaac tggtgcaaat atcttgtagt ctttttttag
     3661 ataattcaga ccttcattga acttagatga atctaatttt agtttcattt ttatcacctt
     3721 tcttatattt tagttttact tacttgctat cttgtttaaa tcttaacact ttattttcta
     3781 tgcttccgtg ataattatca cagtttcaaa aataataaaa aaaatattaa tggagtttat
     3841 taagttctgt taatattttt ttattatcta cattattcaa tttcaaatct aatcacttaa
     3901 at
//
LOCUS       DAELNY010000249         3886 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.252, whole genome shotgun sequence.
ACCESSION   DAELNY010000249 DAELNY010000000
VERSION     DAELNY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.252"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(78..401)
                     /locus_tag="KPE55_003480"
     CDS             complement(78..401)
                     /locus_tag="KPE55_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG0494512.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(394..1095)
                     /locus_tag="KPE55_003481"
     CDS             complement(394..1095)
                     /locus_tag="KPE55_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG0494513.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(1373..2758)
                     /locus_tag="KPE55_003482"
     CDS             complement(1373..2758)
                     /locus_tag="KPE55_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0494514.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(2866..>3886)
                     /locus_tag="KPE55_003483"
     CDS             complement(2866..>3886)
                     /locus_tag="KPE55_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0494515.1"
                     /translation="NYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIENFK
                     KEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNKEL
                     RFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFHELCELSCDEIVIDKC
                     TTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRLNSMFNQDKKKINLGL
                     KFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIEPELI
                     QTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIG
                     RFEIN"
BASE COUNT         1380 a          547 c          463 g         1496 t
ORIGIN      
        1 tcaataatat aacaacttaa attaaaattg tacacatagt tttaaaataa cttatacaat
       61 aaaacattat ccaaatatca caagataatt ccaaataaat atatagatat cactcctgta
      121 ataatagaaa taccaataga tttaaactta tatgcaacaa gaagtacaat cataaaagat
      181 ataattttag ggttagctat agataaatat agactatctc cattaaaaaa tacatcttta
      241 gcagttaagg aagtaagtat tgctacaggt ataaatttca tgattttttt caaaaaatca
      301 ggcatctctt tttttgaaaa aattaacata ggaagcactc ttggtagata tgtaacaatg
      361 tacattccaa ttataataaa aaatatataa ttattattca taagattcct cctttttaca
      421 gtaagattct tctataaaga aaccaattaa agatgctaaa attgatgcaa ctattattac
      481 taaattgctt ttgaataatg catataaaat aacagaaata atgattgatg atataaatac
      541 tacaatataa atcttatttt ttatttgaga aactattaaa gctataaaca tagaagtcaa
      601 tatgaagcca gaaatagaac tgtctataga tatactttct cctaaaaatg ctccaataaa
      661 attagctaaa actgatgaaa ttaagcagaa gaaattaaga taaatagcat ttctatcaga
      721 ccaatttcca gcagtaaatt tttcataatt aactgcaaaa gtttcatctg ttatagtatg
      781 acaaaatggc aatataaatt ttggactttt attttttatg tacatgttca aattagaact
      841 cattagaaaa tgtcttaagt taactataaa tgtagtcaaa acaatagatg ttacacttgc
      901 accagtagaa atcattgaag ctgcaatgaa ttgagaactt cctccaaata ctaaagttac
      961 cattagtgct atttgaaaag gtgaaagacc agatttttgt aatagtattc ctcctactat
     1021 acctataggt atataaccta agaatatagg aattgaagca ataaagcttt cttttaattg
     1081 ttgagttctt tccacaataa atcactcctt ttgtattaaa tgtattaaag tattcgtaaa
     1141 acagaaaaat agattaattg tataattgca attataatta aaaataaatt tggattaata
     1201 tataaaatac tatacaacaa atagataatc ttaaacggtt attaaatttt atctatttgt
     1261 tattatgatg taataataca tattaatctc tataagttaa ttactatctg gtgtaatttt
     1321 attttaacaa caaaaaaaaa tacacacaat taaaatattg tgtgcaatac aattattttt
     1381 tctcttctaa tagtcttttt aatacgctca aaccagcttt aagttcttct gtactttctg
     1441 tagcacatac agctattctt attgcagaaa ttggtttttc ttttccaaca gcaaaacgtt
     1501 cagaagcata gacctgtaca ccttctttaa gtgcttgtat ctcaaatttt tctgccatta
     1561 ttccatcagg tagaatcaac catctaaata tacattcttc atttcccaaa agattatatc
     1621 cagataaata ttgattaata atcttgtttc ttcttcttgc agatttacgg cgtgcattaa
     1681 ttaatttatc ggcttcacct gatgtaatca tgcgataagc aatttctgtt aaaaaataag
     1741 attgagatag attaatgttg tataatgcgc ttgataatgc ctctcgatac tgtttaggtg
     1801 tagagatata tgctagccgt aaactaggtg caataatttt tgataaactc gtaatataaa
     1861 ttgtttgatt gggcagatag gaagccactg gatttaaatg tgtttcatta agtaaactat
     1921 gaatagcatc ctcaataaca attaaattgt atttagatgc aatatttgca atcatttttc
     1981 gtccattttg tgacattata tgtgtagttg gattttgata atctggtatt atgtatagac
     2041 ctttaatatt ttcattttta caagcatata atagtccttc ttcactaatc tcattatctt
     2101 cttgtttaat tggaattagt tgaatcccaa gcattttagc tgctgtcttt attccaggat
     2161 aagttaatgg gtctacacct attctatctc catgttgaaa caaaccagct aaaatagcaa
     2221 caattgcatt ttgtccacca cttgcaggaa gtaagctatc tgatgtagtt tcaagcccag
     2281 ctttataaat taatttggca atagcttctt tttgccattt agcaccatct ctttgtccat
     2341 attggaatat ggttttaaaa cctatttcct taattatgtg tttaaacaaa gtattcattt
     2401 cttctagcgt gaaatctgga ttcattgtgc ccatttcaat aaatgtaatt ttattattac
     2461 tggacatcaa gaatagattt gaacgtgtat cataagatac aaaagttcca cttcctgtaa
     2521 ctccacttat tagacctttt ttttcacaaa ttttaaaagc tctagagata gtactcacat
     2581 taacatctaa gaaatctgcc aattcacgtt ggggtggtaa tttagtacct ggtaataaaa
     2641 atccattgtt gatatcttgt tctagttgtt ctgcaagcgc cttatataat atttgccctt
     2701 ttgaaggtct ctttggtttc cagttcatag gataattttc aaaagaatta attggcataa
     2761 gttttcacct cacattatta atttgatttt ttctttatct aaaacaataa attctaaaga
     2821 tacaaataaa aactgatagg aaataattct atcagttttt gtcttttaat tgatttcaaa
     2881 tcttcctata atttttatac cttctttatc ataaagaggg attgtttttt taaatatatt
     2941 aaaatttctt tttcttgtat ataaaacagc ttcttctggt gtctttggtt gattatcaca
     3001 atcgtacaaa tctctttttt ttacgtagcc gactttttta cctctaccta ttgtttgtat
     3061 aagttctggt tcaatggaat ttccatttga gtctattata tgtgtaccat aggtttctcc
     3121 atttttatta taattaatct taccaatagt atattctaca tcttcatttt gtttaaaatt
     3181 ggagttgata gaaaatattg accctattat tataaaggtt aataaaaact taagacctaa
     3241 attaattttc tttttatctt gattaaacat agaatttaac ctctttttta aaataatatt
     3301 ttttttattt agaaactgag atacgaatga tgaatacttc aaagattttt gatactttat
     3361 tgtatctaat attatatatg catactcttt aatttcactc gtggtacact tgtcaatcac
     3421 aatttcatca caagataatt cacataattc atgaaaatat ttttttaata agtataaaat
     3481 tggattgaac caatatatga tacaaattat actaattaga agcttaaata agttatcttt
     3541 tcttttataa tgaattaatt catgtttaaa gatgaatctt aactctttat tattgtactt
     3601 tttttctgga attattattt tacagcgtgt taaacctata atggcaggag aataaatacc
     3661 tttaacctta tatatctgaa gttttttatt tatacttagt tcttttgctt gttcatctaa
     3721 gatactagta atatctattt ctaaaatttc ttttttaaaa ttttctatgt aattataaaa
     3781 tttaaaattt gttcttaaat agtaaaaaga gattgctata attcctaata tccagatgta
     3841 cataaatata tctaaagtaa tgccctggtc atatggtata taatta
//
LOCUS       DAELNY010000250         3864 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.253, whole genome shotgun sequence.
ACCESSION   DAELNY010000250 DAELNY010000000
VERSION     DAELNY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.253"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            431..1324
                     /locus_tag="KPE55_003484"
     CDS             431..1324
                     /locus_tag="KPE55_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG0494516.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1324..2718
                     /gene="gltA"
                     /locus_tag="KPE55_003485"
     CDS             1324..2718
                     /gene="gltA"
                     /locus_tag="KPE55_003485"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG0494517.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3084..>3864
                     /locus_tag="KPE55_003486"
     CDS             3084..>3864
                     /locus_tag="KPE55_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG0494518.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKW"
BASE COUNT         1523 a          390 c          749 g         1202 t
ORIGIN      
        1 aatatgaaag ctggtattgc aatagattaa agccttatac acgtttagtt tttaaagaaa
       61 ttagtgttat tatgaaaacc taagttaatt tattaaataa ataattgaat aaaaaaattt
      121 tattatgtat ttataaaatt tttgttacaa atttggcata atatagtata ctttaagtgt
      181 gtattaggtt aaatctataa caaattagac gtatgatata gacttagtgt ttattggcat
      241 ttacaagaaa tttgttaata tcaactattt gtagtttggg cagttataat tattataggg
      301 gggatgtcca atacaaaaca atcaaccaag aactaatatt atattataaa gagttatagt
      361 tttgtgtgtt tatgttttta attaaattta aagtgggaaa aatatatttg ttagaatgga
      421 gaatagcgag atgagtaata aaatagttag taagagacaa ttaacagata gtatttattt
      481 aatggaaata gaggcaccta gagttgcaaa atcttctcaa ccaggtcaat ttataataat
      541 taaaaatgat gaaaaaggag agaggattcc tcttacaata gctgactatg atagagaaaa
      601 aggaacagta actattgtat tccaaacagt tggtgcttca actaaaaagt tagcaatgtt
      661 tgaagaaaac gattttgtaa tggattttgt agggccatta ggacaagcaa gtgaatttat
      721 acatgaagat atagaagaat taagaaataa aaagatatta tttgtagctg gtggagttgg
      781 ttctgctcca gtttatccac aagttaaatg gtttaaagag catggattag atgtagatgt
      841 aataattggt gcaagaacta aagaattaat tatattagaa gatgatatga aaaaagtagc
      901 taaaaatgta tatgtatcaa cagatgatgg tacatatgga tttaatggaa gagttacaga
      961 cctattaaaa gatttagttg ataatcaagg taaaaaatac gaccaagcaa tagtaattgg
     1021 accaatgata atgatgaaat ttatgtgcca attaactaaa gaactaaata tacctactat
     1081 agtaagttta aatacaataa tgatagatgg aacaggtatg tgtggtggtt gtagagttag
     1141 tgttggaaat gaaactaagt ttgcatgtgt agatggtcct gaatttgatg gtcatttagt
     1201 tgattttgat caagcaatga gaagacagtc tatgtacaag actcaagagg gaagagctat
     1261 gctaaaactt gaagaaggcg atagtcatca tcatagtaat tgtggatgtg gaggtaacaa
     1321 ataatggatg ctaaaaaagt aaaagtacca gttagagaac aagaacctgc agtgagagca
     1381 acaaattttg atgaggtatg cttagggtat aataaagaag aagctatggc agaagctaat
     1441 agatgtttag cttgtaaaaa accaaaatgt gtgggtggat gtccagtagg aatcgacata
     1501 ccagggttta taacaaaaat taaagaagat gatatagaag gtgctgctaa agtaatagct
     1561 aagagcagtt cattaccagc tgtttgtggt agagtatgcc cacaagaaag tcaatgtgaa
     1621 ggtgtatgta tacttggaat aaaatcagat gctgtatcaa taggtaaatt ggaaagattt
     1681 gtagcagatt ggtcaaaaga aaatgacata aatttatctg atacagagcc taagaaaaat
     1741 caaaaagtag cagtaatagg aagtggtcca gcaggtctag cttgtgctgg tgatttagct
     1801 aaaaaaggct atgatgtaac aatctttgaa gcaatgcatg agccaggtgg agttttaact
     1861 tacggaatac ctgagtttag acttccaaaa caagctgttg ttcaacctga aatagacaat
     1921 ataagaaaat taggtgtaaa aatagaaact aatgtaattg ttggtaaaac aattacagta
     1981 gatgaactta tagaagatga aggatttgaa gctatattca taggttcagg agctggtctt
     2041 ccaatgttta tgaatatacc aggggaaaat gcaaatggag tattttctgc aaatgagttt
     2101 ttaactagag taaacttaat gaaggcttat agagatgatt atgatacacc tattagttct
     2161 ggtaaaaaag ttgctgtagt aggtggtgga aatgttgcta tggacgctgc tagaactgca
     2221 ttaagattag gttcagaatc atatatagtt tatagaagaa gtgaaaaaga gcttccagca
     2281 agagctgaag aagttcatca tgcaaaagaa gaaggcatta tatttaatac attaacaaat
     2341 cctaaggaaa ttttagtaga tgaaaatgga tatgttaaag gtatggtttg tataagaatg
     2401 gaattaggag agccagatga ttctggaaga agaagaccta tagaaataga aggttctgaa
     2461 tttgtacttg atgtagacac agttataatg tcacttggaa caagcccaaa tccactaata
     2521 tcttctacaa caaagagtct tgatataaat aagaagagat gcttaataac agatgaaaat
     2581 ggtcagactt ctaaagaggg tgtatttgca ggtggagatg ctgttactgg agcagcaacg
     2641 gtaatatctg caatgggtgc tggtaaaaca gcagcagcat caatagatga atatttaaaa
     2701 gcaaaagtta atgcttaatt tgtaaattaa agagagggtg tctcaaaatg aactttttag
     2761 ttcatgagac actctttttg tacaaaatca aatatgtttt aatcatttca acaatgaaaa
     2821 tttcattcct gtagaaagtc taacttttag gtcttagtac aatctctatt ttgatgtagc
     2881 cactttttta gatggtttaa taatttatca gttattattg taatatttga tactaagtaa
     2941 cataactttg tctaactatg ataaaatagt tatacaataa aggtcttgta aggagtgaat
     3001 aatgtgtggc taataaaatc agatttgtca aatctaacat taaataatag aaaaataaat
     3061 tgtgtaatta atggtatttt aatatgaaaa aaagagaaaa agtatttgat gggctagttt
     3121 ttaagcatat attaatctta ttaatactat tagttttgat tgatttaaca agtaccatca
     3181 tatatgcaag taactctatg gaaattactt ctagaaatat gattgaggca tcaaaaagag
     3241 aacttgaaaa ctatttagaa gtaaacatat ctttattaaa agctcttagt caagatgata
     3301 gattttctaa tggtgaaaca tcactaattg aaaagggaaa attattaagt ccatatcaaa
     3361 aggaatataa tctttttatg attggtataa cagatacaaa aggaaatgct tcaagtacat
     3421 atagggaaaa tgttaactca ataaaagata agcctagttt tgagaaagca attaaaacaa
     3481 agcaagtagt agtttcagat attgaagtca gtaatgtaac aggtgacaaa gtatttatca
     3541 tatatgtacc aattgtaaaa aataatgaga tgattgggac catttttgct tctttttatt
     3601 ttcaagatgt aaataattta ataagtagaa gcaattttga tgattctatt aaatttctaa
     3661 tgatagataa agattatact attatttctc atccaaacaa aaaatatgta aatgataaaa
     3721 gtaagatgct tgatttagaa gggaatatta taggaaccac taaaagtgaa atcttaaaaa
     3781 atataaataa aaaatgtcaa ggtgattttt tatcttggga taattggaga ttatataatg
     3841 taaaatatac gaacataaag tgga
//
LOCUS       DAELNY010000251         3664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.254, whole genome shotgun sequence.
ACCESSION   DAELNY010000251 DAELNY010000000
VERSION     DAELNY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.254"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..188
                     /locus_tag="KPE55_003487"
     CDS             <1..188
                     /locus_tag="KPE55_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG0494519.1"
                     /translation="AKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAPK
                     RVKVCTRCLRSGKIERA"
     gene            complement(348..500)
                     /locus_tag="KPE55_003488"
     CDS             complement(348..500)
                     /locus_tag="KPE55_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG0494520.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(598..1074)
                     /locus_tag="KPE55_003489"
     CDS             complement(598..1074)
                     /locus_tag="KPE55_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0494521.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1293..2054
                     /locus_tag="KPE55_003490"
     CDS             1293..2054
                     /locus_tag="KPE55_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0494522.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2314..>3664)
                     /locus_tag="KPE55_003491"
     CDS             complement(2314..>3664)
                     /locus_tag="KPE55_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0494523.1"
                     /translation="LIELQRVVLSEINKFNINFLDFNLKNFIIHLAITVSRILDGYCL
                     DNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVYLYNHFITKSSLLDNVSNRV
                     DTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKSILNSKSYNLNKVNPLINTI
                     KSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIGAGIERFFQSNIKCKNVVLV
                     CGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASELSDVDIIISTIPLNHDSIPI
                     VLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLFIVNPKIKDKDELIKLMCNK
                     LTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELSSIRTKICISILNEPIYWSE
                     NSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDSISNCNDFDNFLSIIKSIV"
BASE COUNT         1384 a          504 c          440 g         1336 t
ORIGIN      
        1 tggcaaaagt atgtagcgta tgtggtaaag gtaaggtttc tggaaaccaa gtatcacact
       61 caaataaaca taataaaaga acatggtcag ctaacttaag aagcgtaaga gcgattatag
      121 atggagctcc taagagagta aaagtttgta ctagatgttt acgttctggt aaaattgaaa
      181 gagcttagta accacgttta aaagcctact tccgtaagta ggctttttaa tttaatcatt
      241 tttactcttt ctcataaatt ttaggattat acttgaaatc cattttggta attttattgt
      301 tactactttc atcaatactc accccacact ttatagttaa aactatttta acaaaaaaat
      361 aataatgctc cacatccaat aagtattaat gcggtaagtg ctgaccaaat ccagccagga
      421 agcaacattg acagaattac agccattcct gttgctagac aaagtattcc tagcaatacc
      481 ttagacttgt tatttctcat ggaacacctc cacatagaac ctgtatctat aaatattttc
      541 atattcaagt ttatgcgaca gttttccatt ttagaccaat aaacacatta tgtaatttta
      601 atttttcata ctagaattat tactgaactc cgatatagat tctacaatat ccaaaattcc
      661 tttttttcta cttaaccttt caggtccact atataaaaca actggtataa ctttaggaag
      721 tttaaaattt ttatcttcta tttcttcaag gctttttttc ataataaaac ttttccaaat
      781 ttgttctcta tacgatttca atcttgggac aatttcagta tccatttctg tttgaaattc
      841 taacactata taatgtgata ctttgccgct tttagaaaat accttataaa ttatatcaac
      901 ttcttttcct ttatattcac atataaaaga tgtttctaac tctatatttt taagagttaa
      961 ttcttttgct attgagacac ttgtaaaatt ttgaatcaaa cttaaaaaac tttctttatc
     1021 tgaatacatt cttctgtact cttctcgttt ttcattgctt ctttttaaat tcatacagtt
     1081 cctcctagac tatagacctt ctatttttta gtaaagatag tctataaatt ttatttttta
     1141 attttcacta gtgagtattg acagttgtgt gtaaaatatc cagtaaagat atttttttat
     1201 atttttatta aaagatataa atttttatat tttaatgtat tgacatttac gttgcgtaaa
     1261 agtgtatatt taatttatag gaggtactac ttatggaata tacagttcaa aaattaagta
     1321 aactagctgg tataagtact agaacactta gatattatga tgaaataggt cttcttaagc
     1381 cacttaaaat aaattcttca ggttatcgta tatatggtca aaatgaagtt aataaacttc
     1441 aacaaatact tttttataga gaattaggaa ttaacttaga aaatataaaa aacattataa
     1501 attcaccaaa cttcgatagt ttaagtgcgt taaaagaaca ccataataaa ttactagcca
     1561 aaagaaagca aattgattta ctaatagaaa atgtaactaa aactatagct ctgaaggagg
     1621 gaaaatacac tatgacagat atagaaaaat ttgaaggttt taaagaaaag atgattgatg
     1681 aaaatgaaaa aaattatgga actgaaatta gagagaaata tggtaaagat attatagata
     1741 catcaaataa aaaacttaaa aatatgtcta agcaagatta tgaaaattgg caaaacctaa
     1801 atatcgaaat aattagtaaa cttaaaaaag cttttaaaac tggtgatgcc tctagtgaac
     1861 tggctcagga agttgcaaaa ttacatcata aatggttgtg ttacacttgg aacacatact
     1921 ctaaagaagc tcattcctct ctagcacaga tgtatgtata tgatgaacgt tttacatcat
     1981 attatgatag agaacaacct ggattagcta agtttttaag ggatgctata gtaatatata
     2041 caagagataa ataatataaa ataataaagg tggtgtctca aaatgaactt tttagttcat
     2101 gagacactct tttttgtatg aaatcagata tatttttatc attttatcaa tgaaaaacag
     2161 gtttctctct ctattttgag acagaatctt tattgtataa taattataat caatacaaaa
     2221 taataacaaa ttatacaaaa cttacttata ctatttatgc taaaaacaaa aaatatttta
     2281 ttaaagtttt taaaacttat gtaatataaa aatctatact atagacttga ttatggaaag
     2341 aaaattatca aaatcattac aattagatat actatctcta atatcattgt catgaattat
     2401 ctttaaaagg atatcataaa tggagtttat ttttatataa tcatctttat ttatagccaa
     2461 catcatgatt aatttaacag tagaattttc actccaatat attggttcat tcaatatgga
     2521 aatacaaatc ttagttctta tagagcttaa ttccatagga tgaggtatag ctaaaaaatc
     2581 atctatattg gtagaagata aactttctct gtaaaataca gattcagcaa atgatggaaa
     2641 tacaatttca ctttgagtta atttgttgca catcaacttt ataagctcat ctttatcttt
     2701 gattttagga ttaacaataa ataaattttt atcaaagaaa ttgtccaaaa gattactata
     2761 aatatgggaa ttattagtga ttgacttaga tatattctct atatcttttt ttaatagttt
     2821 taaatccact aatacaatag gaatactatc atgatttaaa ggaattgtcg atattattat
     2881 atctacatct gacaattcac tagctaaaaa ttgattaaat gatagacatt gaagtatatt
     2941 tattttatca tgaaaaactt tgtttaattg aacctctaat aacctacttg aaccataccc
     3001 tgaaccacat actaatacta cgtttttaca cttaatatta ctttgaaaaa atctctctat
     3061 tccagcacct atatgcaggg atacatatcc aatttcatct tctgttaaag agtaaatact
     3121 atttttaaaa actttttcta tagcatttaa agttatttca aatgctaaag gataattact
     3181 ctttattgta tttataaggg gattcacctt atttaaatta taagatttag aattgagaat
     3241 tgatttaaag tgcagtacta aatcatcaaa taatacacta tcatttctaa ggtcaaaagt
     3301 atattgattg ttgattactt ctaaaatttc ttcaacatac tctataattt tagtatctac
     3361 cctattggaa acgttatcca aaagagaact tttagttata aaatgattat atagataaac
     3421 tctgtctgcc tttgaaataa ttatagtata tttactctca atataattaa atatattttc
     3481 aacagttgta ttcgattgaa aatctgttaa aacaacatct aaaacattat ctaagcagta
     3541 accatcaagt attctactta cagtaatagc taaatggata ataaagtttt ttagattaaa
     3601 atctaggaaa ttgatattaa atttattaat ttcacttaac actacccttt gaagctctat
     3661 taaa
//
LOCUS       DAELNY010000252         3637 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.255, whole genome shotgun sequence.
ACCESSION   DAELNY010000252 DAELNY010000000
VERSION     DAELNY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.255"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            193..1773
                     /locus_tag="KPE55_003492"
     CDS             193..1773
                     /locus_tag="KPE55_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG0494524.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTIN
                     ATDLISNISLTMKELLDI"
     gene            1947..2393
                     /locus_tag="KPE55_003493"
     CDS             1947..2393
                     /locus_tag="KPE55_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG0494525.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2414..3457
                     /locus_tag="KPE55_003494"
     CDS             2414..3457
                     /locus_tag="KPE55_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG0494526.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1463 a          336 c          687 g         1151 t
ORIGIN      
        1 cataataact taaattatat ggtataataa attaaataat tgttttaatt caaaattttt
       61 tgttatttta aattatttca aaactcttag ttttagtgat tttatttatg atatttatgg
      121 gattaaaaag atttcaatag atatttttgg taagtattta agaagtcttt aactttatag
      181 gagtgataat ttatgctaat aggtacatct aaattaagca gaaatgttga tactgcatgt
      241 gtagaagaag tagggattcc aatgattgta ttgatggaaa atgcggttat atctgcaatg
      301 aaaaatatgg atatagacat gtataatagt tatacagttg tatgtggtgt tggaaataat
      361 ggaggagatg gtcttggaat agcaagacat ctaaatacat atggaaaaga agtaaacgta
      421 tttttagtag gtaatcttga aaaactaagt gaatgctcta aaataaatta caatgtactt
      481 ttaaatatgg gaattaatat tataaatatt aataaaaatg atttaagtga aaaaaattat
      541 ggagatgaaa atgaagtaga taaaattatt aaagttgaga aacaaaatat tataaaaaat
      601 aaaaatttaa caatttttaa agatagagta tctaaaagtg atgttgtagt agatgctata
      661 tttgggactg gattaaaacg tgatataact ggtattttta aagaagttat tgacatagta
      721 aacgataata gcaaaaatac gtattctata gatattcctt caggtataga tagcgataat
      781 ggaaatgtat taggaacttg tattagagca aacaaaacga ttagctttga gttttataaa
      841 agaggttttc taaactatga cactagcgca cttataggtg atgtagttgt agaaaagata
      901 ggtgttccag attttattac aagaaaatac catgatgaag agttcataac tgataagact
      961 tttgtaaaaa ataatattag aagaagagat aagcatggtt ttaaaagtga ttttggaaaa
     1021 gtatctatag ttgcaggttc aaaaggattt tatggtgcaa gtttcatagc aactgaatct
     1081 gctgttaaga gtgggagtgg acttgtaaca ttaattagta gtgaagatgt gcaagaaaag
     1141 gtatctatta gacttacaga agcaatgact ttaaattttg aagaagaaag attaaataaa
     1201 ttacttaatt ctagtgatgc aattggtttt ggtccaggta tgggagataa tagtcaaaca
     1261 tttgataaat tacttaagat agtagaaaat tctaattgcc caatagtatt agatgcagat
     1321 ggattgaacg ttatgaagga cagatgttat aagtttttag aatggaaaaa tagatttgta
     1381 attacacctc atttgggaga aatggccagg ttgacaggag attcaattgg gtatataaga
     1441 gaacatagag tagatgttgc aaaagaattt gctcaaaaat ataatctggt ggtgctttta
     1501 aaaggttatc aaactgtcat aacagatggt aaaaaaacat atataaatcc tacaggaaat
     1561 agctgtatgg caactggtgg aatgggtgat tgtttgcttg gaatgattac ttcatttata
     1621 ggtcaaggta tggatatatt ggaagctaca gttagtggag catatataca tggctatata
     1681 ggagataaat tgagcaaagc aatgtatacg ataaatgcta cggatttaat aagtaacatt
     1741 tcattaacaa tgaaagagtt gttggatata taattggtga tatttgatac tactttgtaa
     1801 caattttaac aaagtatgtt gatataatac tattaattaa ttaatttatt ttattgtttt
     1861 gtaatttaaa tttatattca aaaacattgt ataaacatat aatttatata gggttttata
     1921 attcatatga aaagagggta gatagtatga agttaatatg gttgatagta gcagtcttat
     1981 ttggaatagc agaaatgtta acaccaagct taacattgat atggtttagt gtaggagctg
     2041 taatacttat atttttaagt agctttatag agagtatttt cttacaaatt ttaatatttg
     2101 cagtaatttc tatagcaatg ttagtcgtag ctactaaaaa aatagtaaaa aaagataaag
     2161 gctataaatc taacactaat ttacaagcaa tgatgtctaa aaaaggtatt gttacagaag
     2221 agatttctcc aaataacaca ggtctagttg tagttgaaca tgaaacatgg acggctattt
     2281 ctatagatgg tgaaaagata gaaaaaggtt ctacagtaga agtattaaaa attgaagggg
     2341 taaaattagt tgttaaaaaa gtagacgcca cagttaatgt gacaaaccaa tagaataaag
     2401 gaggatttat agtatgggta ctaaaattgt tttaagtatt gtattaatag tagtagtcgt
     2461 agcaataagt ttaacttgta taagagttat aaaacaatct aaagtaggta taataatgag
     2521 acttggtaag tttcaaaaag tagctgaaac aggtgtacat ttcttaatac catttttaga
     2581 taaaatggca tatgtaattg accttagaga aattgtaata gattttccac ctcaaccagt
     2641 tataactaaa gataatgtaa ctatgcagat tgatacagtt gtatattata aagttacaga
     2701 cccagttaga tatgtgtttg agatagctaa tccaatagct gctattgaaa atttaacagc
     2761 tacaacacta agaaatataa ttggtgaact tgatttagat gaaacattaa catcaagaga
     2821 tataataaat gtaaaaatga gaactattct tgatgaagca acagataaat ggggtataaa
     2881 agtaaataga gtagagttaa aaaatataat gcctcctcag gatattcaag ttgcaatgga
     2941 aaaacaaatg agagcagaaa gagaaagaag agaggcaata cttcaagcag aaggtaataa
     3001 gtcagctgca atacttcaag ctgaaggtga aaaacaatct gctatattaa cagcagaagc
     3061 caaaaaagaa gctatggtac gtgtagcaga aggtgaaaaa gaatctgcta tattagtggc
     3121 agaaggtgaa gctgaagcta taagacagac agctattgct aaggcacaag gtgaagctga
     3181 aatgataaaa agaactcaaa tggcaacagc agaaggttta aaattagttt tttcagcaat
     3241 gaaagaggct gatatagaca ataatattct agcattaaaa tctatggaag ctcttgaaaa
     3301 aatggctgaa ggtaagtcaa caaaactggt tttaccttca gaagcagtta atttcctagg
     3361 aacattcaaa ggaataaagg aagttatgag tgatgataac aaagaagtac ttgatataaa
     3421 agaagtttta aatgataatg aatcattaaa aaaataaaag agttttgtta tcataggaag
     3481 gatatggaaa ttaaaggtcg aattaattaa atatagcaca aaaatgcacg ttgaaaagaa
     3541 gaggacttat aaatttttaa gtcttcttct ttgtttttat tagaaagatg aaattataaa
     3601 aataaattaa cagaatcttt atacaattta tccttta
//
LOCUS       DAELNY010000253         3502 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.256, whole genome shotgun sequence.
ACCESSION   DAELNY010000253 DAELNY010000000
VERSION     DAELNY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.256"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            140..982
                     /locus_tag="KPE55_003495"
     CDS             140..982
                     /locus_tag="KPE55_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0494527.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            1285..3357
                     /locus_tag="KPE55_003496"
     CDS             1285..3357
                     /locus_tag="KPE55_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0494528.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         1367 a          338 c          630 g         1167 t
ORIGIN      
        1 acaatatata agcgttaaca agctggattg ttactggtaa gcaggcaata cctaggttaa
       61 ggtatacagg tgaattgtat cttaatttag gtattttttt atgaataagg gaaattgtat
      121 tttgggagag atattgatta tgattattca acaaatttat aataataatg ttgtattagt
      181 tttagatgaa aatataaaaa aagaattaat attaacagga tgtggaattg ggtttcaaaa
      241 aaagaaaggt caagaaatag ataaaagtaa aattgaaaga acttttgtaa tacaagatga
      301 aagcttttta gataaaatta gtaaactagc aagccaggtt gatgagaaat tttttgaggt
      361 tagtactgaa ataattgctt atgcagagga gaatttgaat actaagcttt atgaatatat
      421 ttatgtagca cttactgacc atattgcatt tgcaattaaa agatatcatg aaaacataac
      481 tataaagaat gatttactac atgagataaa aagaatacat aaaaaagaat atgagattgg
      541 gaaatgggct gtagattata taaataaaga atttgatgtg aaatttccag tagatgaagc
      601 tggttttata gcaatgcata tagtaaattc aaactataag ggaagttcaa aggaatcact
      661 attaattact aaaattgtaa aagacatact aaatattata agatattatt atagagttga
      721 atttaaagaa gatgatataa attatgatag gcttctgact catttaaaat tctttgcaaa
      781 acgattagta aaaaaagaaa aaatcaatga tacaaataat gaaattatag atataattaa
      841 agtaaagtat gaaaaggatt atgattgtgc atataaaata aaaactcatg tagaaaagaa
      901 ttatgattac tatgtaagtc aagatgaact actttattta acattacata tcaaaagagt
      961 tatatctgta ctgaatttat aataaagagt agtttaatac agaatgtata gatttaatat
     1021 attatgttta attttaatat attatttaaa atttaatata aaataataat tttaaaacat
     1081 taagattgtt actgataatg caggcaagac ttatgtttat aaaaaaatta taatagttta
     1141 tttttaattc tgaaattagg ttaatatagg tttatttata ttaacataag tttaatttta
     1201 gaactaagta tactaatgta actttttata aacttaagtc ttttttattt aaaaaaatat
     1261 aaaataataa ggagagaaaa gtaaatgaaa aaagtatttg gtgttttaca aaaagtagga
     1321 aaatctctaa tgctaccagt tgcattactt cctgcagcag gtatattact tggcgtgagt
     1381 aatgctttag caggagacgc attgttggct cattttccag ctttggcgaa tcctacattt
     1441 caacttataa taagtataat ggaaagttca ggtcaaatta tatttaataa cttaccactt
     1501 atatttgcag ttggggtagc tgttggttta actgaaggag aaggtgttgc agcattggca
     1561 gccatagtag gttttttaat tctcaataca tctatgggtg ttatggcagg tgttacagaa
     1621 gatatggtag acaattctat gtatgcaaat attgttggaa taccaacaat tcaaacaggg
     1681 gtatttggtg gtattattat agggatagtg gctgcttatc tttataaaaa gttttataaa
     1741 attgaattac ctcagtatct aggttttttt gcaggtaaaa gatttgtacc aatcgttact
     1801 tcattagttg ctatagcaat aggaattata ttgtctttta tttggcctcc aatacaagag
     1861 ggattactta gtttctcaaa aaatgttata gaagcaaatc aaacactagc agcatttatg
     1921 gaaggttctt tggaaagact attaatacca tttggtttgc atcatgtttt ccataatcca
     1981 ttttggtatc aatttggaga atatgtaaat aaagctggtg agcttgtaat gggtgaccaa
     2041 aagatattct ttgctcaact taaagatgga gtaaatttca cagcaggtac ttttatgaca
     2101 ggtgcatttc catttatgat gtttggtctt ccagcagcag ctcttgctat ggtgcatgaa
     2161 gcaaagccag aaaagaaaaa atttgtagca ggtataatgg catcagcagc attaacctct
     2221 tttttaacag gaataaccga gccaatagag tttgcgtttt tatttgttgc accagtatta
     2281 tttgcaatac attgtttatt ttcaggttta tcatacgcaa ttatgcaatt attaggagtt
     2341 aaaattggat tcacattttc aggaggatta atagatttct tattatttgg tgttcttcct
     2401 aatagaactc catggtactt ggctatcttg gtaggtattg gttttgcagt attatactat
     2461 agtgtattta gatttatgat aagaaagttt aatttaaaga caccaggaag agaagatgag
     2521 tcagaaacta acgctgaagt cattgaaatg tctaagaatg aattagcatc agagattttg
     2581 attgctttag gaaataaaga caatttaaag agcctagatg cgtgtataac aagacttaga
     2641 ttggttgtaa atgatataaa taaagtagat aaagaaaaat tgaaatcact tggagcagca
     2701 ggtgttatgg ttattggcaa caatgttcaa gctatatttg gaccaaaatc ggattcaata
     2761 aagtctgata taaaagatat aatagaggga aaaacaaagg ttgaaagtac tgttactaaa
     2821 aatgttgata aaaataaagt gaaaaaaaat atggaagttg caaatatgga aaatgcttta
     2881 gtagaaaaga ttgattcatt aacaaatgga gagatattag atatagaaga agtgccagat
     2941 tctgtatttt caagtaaact gatgggggat ggatttgcaa ttaaatctag tgatggattg
     3001 atttattcac cagttgatgg aactattgga gttatatttc caactaagca tgcaataata
     3061 ataaaatccc aaaaaagtgg agtagaaata cttatacatt tagggataga aactgtaaac
     3121 ttagaaggaa atggatttga agtatttgtg aatgtaggag atgaagtaaa agcgggagaa
     3181 aaattagtta aaatggactt agaatatatt gaaaaaaatg gtctatcaac aataagtcct
     3241 gtagtattta cgaatttaga acaaaatcag aaattaagca ttaaaaaagg agtaaccaca
     3301 gcaaaagagg agaatagagt aagtatatta agagatgata ttgaaaaaat aggatagcta
     3361 tatgatagtg taactaaaag ttatgtgaga tttaataaat atataaatag gttcagttaa
     3421 catgcaattt atttaatata tattttaagt aatagaaata tatatcatta tattttgata
     3481 tattttaaaa cattaaaaaa ct
//
LOCUS       DAELNY010000254         3500 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.257, whole genome shotgun sequence.
ACCESSION   DAELNY010000254 DAELNY010000000
VERSION     DAELNY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.257"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            627..839
                     /locus_tag="KPE55_003497"
     CDS             627..839
                     /locus_tag="KPE55_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494529.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELELGEYGVLGWTNEGLYLH"
     gene            complement(1003..1203)
                     /locus_tag="KPE55_003498"
     CDS             complement(1003..1203)
                     /locus_tag="KPE55_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0494530.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1429..1605)
                     /locus_tag="KPE55_003499"
     CDS             complement(1429..1605)
                     /locus_tag="KPE55_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494531.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1660..1757)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1998..2381
                     /locus_tag="KPE55_003500"
                     /pseudo
     CDS             1998..2381
                     /locus_tag="KPE55_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      2737..2830
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2913..3398
                     /locus_tag="KPE55_003501"
     CDS             2913..3398
                     /locus_tag="KPE55_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0494532.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT         1430 a          391 c          473 g         1206 t
ORIGIN      
        1 aatacaatca cctataatta aatttttatt aattatattc tagcatcaaa tattgaataa
       61 gtcatcttct ttaaaatata ataaatagta ttttgctcaa attttcaaat gcttctctgt
      121 ccatctcttc taaaaactga gagtagctat tcatagttat ttttatatct gcatgaccta
      181 atctttctga aatagttttt atattagttc cagaaagaaa cattaaagtt gcattattat
      241 agaatttaaa cataattaaa tgtaaaaatt aattgaaaat attaattata tgttatgata
      301 taataaaaat atagaaattt tgcagtgagc gatatttgtg acaaattgag gtttagcagt
      361 tgaaatataa ggcattgaga atatataata agtattatca attgcactat tgcgcgttca
      421 ctgcaatttt aagagcattg tatatgtgta aacattggag atgctaagtt tattttgggg
      481 ttttatatca actatgtggt atgtaaagag aaaagtttcc agtaggaata cctaagtata
      541 ttaagtttta tatcaactat gtggtatgta aagagtttta acacctgccc tttatagatg
      601 gtgtatttac gttttatatc aactatgtgg tatgtaaaat ttttattaca ttaaaaacaa
      661 ttctcccaaa aacacaaata attgtcacaa cacacagtcc tcatatttta caaattgatt
      721 ctaaagaaga aatgattgtg ttagatatgg ctgaaagtga taatgtatat aaaaaagagt
      781 tagaacttgg agaatacggg gtattaggct ggaccaatga aggtttatat ttacattaag
      841 agcactcttt ttataggagt gcttattttt ttgaaattca ttagcatata aactatcaag
      901 aacattactc aatatacctt atttacttca ccaatgatta tcttacatat attaacaata
      961 aaaaaagact ctaaaaagag tctttcccga aaaatctata tattataata tagttatatt
     1021 ttctgcttga ggacctctag cacctttaac tatatcaaag cttacttgtt gaccttcttc
     1081 taatgattta aatcctgaag tttgtatagc tgagaaatga gcaaacacat catctctacc
     1141 ttccattgat ataaaaccga atcctttttc attattaaac catttcacta ttccattatt
     1201 cattaaaaat actcctatta acctatatca agatatactt gatttaattg tgtattacca
     1261 caaatactat tgtatcatag attaatagaa atgtctactt aattatattt tttagttaac
     1321 attttagtta ttcttaatat taaaaatata tttaaacgtt ttacattgct atatattaat
     1381 ataaatgtag ataaaactaa aaagggttct ctagaaatca atgtctatct aattatactt
     1441 acttaccaat accatcactt ggtgtaaaca agactctact acactttctc cgtactctaa
     1501 tataaaatca tctaattgtg tatctaaaaa attacaataa tcatacgaac tgctaatata
     1561 agcagtcaat acatgtgtac atttatccat tttatttcca aacatataaa ttattatccc
     1621 cctaaaaaac tttcataaaa acaaaaagaa cactatctct ttggcaactg gctgacataa
     1681 ctcataagat atttatataa gtcttagtcc ctatagcttt gcgtcactaa atttctctag
     1741 ttttgccgat ttagttttat tctacaacta aaataataca atagaattag ttattattca
     1801 acaggattgt ttgaaagtta aataaattta tagatatgaa cattatttta cacttaaaaa
     1861 caggataaac acgtagagta cctggtaaaa attaaatata gtttttgttc taaaaagctc
     1921 ctattataat ttaagctaga taaatttgaa gaagtgacat aagaagaagt gacataaatt
     1981 gaaagaagga atatattatg ctaaataaaa aattaccaga tacagaatta aaaattatga
     2041 aatgcatatg gaatactggt tataaaactg taatatcaaa agatgttgca gatgaaatag
     2101 aaaaaacata tggttggaag catacaacta caattatact tttagctaga ttaaccaaaa
     2161 aaggtttttt aatatcatag agaattggga ggcatgtaca ctatacagta ttaataaaag
     2221 agagagaata tatcaaaata gaggaaaaaa gagtttttag aggtttacat aacaatcctt
     2281 tatcagaatt aatttcaaaa ttacatgatg gagaagaaat aactgaagaa aaaataatag
     2341 aaataggaag ttggataaaa agctggaaat atgaagatta ataaataaaa ggtagctaat
     2401 aatataagag ctaccttttt tatgtggata atactgggga taagttgtgg aaaacttata
     2461 taaaaattgt ggataaagtg ttttgctgac attttactga caaaagttaa aaaaataaat
     2521 gttatatata ttgtaaaatg ttgaaaaata gccatatata taaaatataa gtaacactat
     2581 agataaatta ctgtctttta tattgactat aaagaacagt aattgaataa atataaagat
     2641 attttttgat tattattaat taaaaattta atttatattt aaataatata aaatttgagg
     2701 taatattatt ttttaattgt tcaaattatg ttattattca gatataggca atattagata
     2761 aatctaatga cgcaaaacta tagggactaa aatctttatg taataaaggt catgtcagcc
     2821 agttgccaaa aagataataa ttctttttgt taaaagtatt tttagagtta tatattttaa
     2881 taacttaata tttggcaaga ggagggctta agatgatatc tgaattaatg ttaaaaaatt
     2941 ttcgtaaatc tagcatcaat tttaagacta aaattaaatt gaatttaaga gaaaaaccag
     3001 acataaatag tttgaaatta aaaagtatac cagaaggtaa aattgttaag ttaaagtgtg
     3061 tagatggaat atgggcagaa gttgagagta attatgacaa aggttggtta ctttataaat
     3121 atttagaaag attaagtaat gctaaaaaca atagtaatga tacaataaaa aatagaaaaa
     3181 agaattacat aggaaatata agaacaaatg gattaagttt agaacttaga aatgatagaa
     3241 cattagaatc taaagttata actacaatcc ctgatggttt taaagttgaa gtatgttata
     3301 gcgttggaaa atgggcaaga gttaatatca ataaaaatga aaaacgatat tctggatatg
     3361 tatataatca atacatagaa gagcatttaa gtttataata cgatactagc aagctttaaa
     3421 tatattaata aatcgcaaaa aagaatccca ttaatgggat tcttttttgt agaaacatta
     3481 taacagaact aaagagaata
//
LOCUS       DAELNY010000255         3496 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.258, whole genome shotgun sequence.
ACCESSION   DAELNY010000255 DAELNY010000000
VERSION     DAELNY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.258"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1277)
                     /locus_tag="KPE55_003502"
     CDS             complement(<1..1277)
                     /locus_tag="KPE55_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494533.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNK"
     gene            complement(1277..2023)
                     /locus_tag="KPE55_003503"
     CDS             complement(1277..2023)
                     /locus_tag="KPE55_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494534.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(2170..>3496)
                     /locus_tag="KPE55_003504"
     CDS             complement(2170..>3496)
                     /locus_tag="KPE55_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0494535.1"
                     /translation="LSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYILKNYYQDNF
                     YLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDHFILLIACN
                     TEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRANIAA
                     KSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVNEIE
                     GAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYTPVP
                     ISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILIDIMKKIKE
                     IGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISKIVEMAKAI
                     DIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
BASE COUNT         1220 a          570 c          316 g         1390 t
ORIGIN      
        1 attttatttt ttgatttttt tacttctata tttaaatttt ttatactata attttttaat
       61 tgttttaaag aatttatatg ttcttgcgaa ttgctactag gaattataat cccttcatat
      121 ttttctaact ttgaactaat tttatctaat cccaattcct ttgataattt gttataattc
      181 tttacactta gtaagacttc cctattatat ttaagtacag gaaaacttaa ctccttataa
      241 tcatatcctt tgattgcttc tctaatttgt tgtagttctt catattcttt ttcattatta
      301 tgctctgaaa aatatgagta aaccattggg aaattatttt tagaagcttc tccttgagaa
      361 cttacaagtg ttgaagttgt tgtaaatgct actaaagtag ccgataaaag tattgttatt
      421 aaaaataaca atcgtgtatt atcctttaat ttatatatca aattacttat aagtaacata
      481 tttactttgt tcttataaaa tttttcttta ttttttaaat actttaatat caaaatagta
      541 aattgagaaa aaaataaaaa tatccccaaa gtaattaata gtgctgttgt atacatacct
      601 ccatttattt ttaacttatt gccaaatagt atcatagcat atcctaatat aagacatatt
      661 acaccaatta ttccaaatag ctttgaagat tttatctctt ttttaggctt ttttgtacct
      721 actaaaagct ctaaaatgct ctttttactc aataatttta agctcattgg tcctgttatc
      781 aaaaataaaa tcataaatga tacaatagtc aatagcattg cttttactgg aaaataaaat
      841 tcagttaaat tcatataaaa cagtttgctt aaatacaata aaaacaattt tgaaattgat
      901 aaaccaagta atattcctaa catcactgct aaaaatccta ttatcaaatt ttcaattaat
      961 atcaattttt tcagttgtct gttactcatt ccaattatca tcaatactcc aaaatctttc
     1021 aatctatatt ttaagaaatt tcctaaagag tacaatataa aaagtagact aaaaccgaat
     1081 attattaatt ctgaagtcat aagagccata aatgcagtag aaccactctg cattccagat
     1141 tttgttataa ttggatggaa catagacact gcaaaaacaa aaaatatcat tatagaaaaa
     1201 acagaactta aaaaatatgc taaatatgcc ctgaaatttc taactatatt attataagca
     1261 aactttctaa aatccactat cttacacctc ctatttgaga caaaacagct agtatatcag
     1321 aatagaaaga attactgctt tcagattttt ttatttctgt atatatacaa ccatctttta
     1381 taaaaattac tctatctgaa taacttgcac tatatgcttc atgagttacc ataagtgttg
     1441 ttaccttttg ttctttgttt atcctagtaa ataatttcaa aacatcatca gtagattttg
     1501 aatcaagatt tccagttggc tcatctgcaa gaagtatggc aggtttattt ataatagctc
     1561 tagcaatagc acacctttga gcttgacctc ctgaaatttc ataggttttt ctatctaaaa
     1621 ttttatctat tcctaaaaat ttagatatac ttttagtttg tatattcata tctttgatac
     1681 ttgccccctc tagtgtaagt ggcaacataa tattttcgcc aacagttaaa gtgtcaatca
     1741 aattaaaatc ctgaaataca aaaccaagtt gtttccttct aaattcagca agtttttctc
     1801 ctttcaattt acttacatca tatccatcta atacaacctc accttcactc tgtctatcaa
     1861 ctgtagatat tatatttaaa agagtagatt taccacttcc agatggtccc ataacagaca
     1921 caaattctcc cttttctata tttagactaa ccttatctaa tgctttaaat ggaatatttc
     1981 cacaatatgt ttttgatata tttctaatac tcaatatgtt cattttatat tctccataat
     2041 tttttatttt tactacaata ccataatatc gtgaatatac tactttaacc ataaaaatac
     2101 cctaacaatt aacttacata tttgtaagta agttgttagg gtatcttata gtctaataat
     2161 tattaatgat taatcaaatt taaagtttaa gtgtttttca aattcatcta ataccatagg
     2221 tttagcaaat aagtatcctt gaaccttatc acaacctatc tctttcaaga actctacttg
     2281 ttcataagtt tctacgccct cacagattac tttaatatca atcgcttttg ccatttccac
     2341 aatcttagaa ataataattt ttccacgttt tgtatctgtt gtttcgatta aaaatcctct
     2401 atctaatttt aaaatatcaa aagaaaggtc ttttaacaga tttaaagatg aatatcccga
     2461 accaaaatca tccattgata taaggaatcc tatctcttta atctttttca ttatatcaat
     2521 caatatattt aaattatcaa acacaacact ttctgttagt tcaagctcaa taaactcagg
     2581 agatatattg tatttactta tcaaatcagt tatattttct ataaaattat ccctatataa
     2641 atgaactcta gacatattta cagagattgg tacaggagta taccctttat ttatccactt
     2701 gtttaatgtt ttgcatactt cttcaaacac gaacatatca atatttacaa taaaaccatt
     2761 cttttcaaat aaaggtacaa aatctatagg tgaaataaac ccaaattctg gactattcca
     2821 tcttatcaag gcttctgctc cttcaatttc atttacatca tctaaactat acttaggttg
     2881 ataatatact ataaattcac ctttttctaa agctttattc atattatctt ccaatcgttt
     2941 ttccttgtac aatcgatttc ttgtatcttc attaaaaaat gaatatgaat gttcatattt
     3001 ttccttttta ctttttgctg caatatttgc tctatcaatt aacaagtcaa tctttttaat
     3061 atcatccttt ttagtaatct tatatattcc acttgacata acaattttat acttagatga
     3121 gttagtagta tttaagttac aaatttcttt atttatttta gataatctat ttaaaagttc
     3181 atcttcagtg ttacaagcaa tcaacaatat aaaatggtca ttatccatcc tagcacaaat
     3241 ttcatattta tttgtattat tatttaaaac ctgtgatata tgaattaata tcttattccc
     3301 ttcagcatat ccaaacatct catttatata tttaaattta ttgacatcca attgtactaa
     3361 gtaaaaatta tcttgataat aattctttaa aatatacata gaatcttttc taaacttttc
     3421 tttatttttt attccactta aagaatcata ataagccaat tttttaagtt gttctattaa
     3481 tttagcatta cttaat
//
LOCUS       DAELNY010000256         3455 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.259, whole genome shotgun sequence.
ACCESSION   DAELNY010000256 DAELNY010000000
VERSION     DAELNY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.259"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(199..1470)
                     /gene="serS"
                     /locus_tag="KPE55_003505"
     CDS             complement(199..1470)
                     /gene="serS"
                     /locus_tag="KPE55_003505"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0494536.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(1596..1801)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1929..2810)
                     /locus_tag="KPE55_003506"
     CDS             complement(1929..2810)
                     /locus_tag="KPE55_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0494537.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(2822..3331)
                     /locus_tag="KPE55_003507"
     CDS             complement(2822..3331)
                     /locus_tag="KPE55_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG0494538.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         1120 a          593 c          329 g         1413 t
ORIGIN      
        1 aaataatttt tagatattca tacaagagtg ttttataaaa atatttagtg ttttatgatg
       61 aaaattctgc tgtgtaaatt aacataaaaa aactatgaaa ttaatattta taataatttc
      121 atagtttttt tatttattgt ttactcataa tatattctct tcatcaaagc atactctata
      181 tatacaatta atataaattt attttataac atcaacaccc atataaggtc ttaattcttc
      241 tggaactact acagaaccat ctgcctgctg ataattttct aatatagcag ccaaacatct
      301 tcctattgct aatccagaac catttaatgt atgaacatat tctgcttttg attttttatc
      361 tcttttaaat cttattcctg ctcttcttgc ttggaaatct tcaaaatttg aacaagaaga
      421 tatttctaca tatctattgt aacttggcat ccaaacttct aaatcatatt taaatgcagc
      481 tgtaaatccc aaatctcctg tacatatttt tacaactcta tatggaagac ctaacatttg
      541 taacatagtt tcagcatcat gagttaatga ttctaattca ttataagaat cttctggttt
      601 aacaaattta actaattcaa ctttattaaa ttgatgttgt ctaactaaac ctcttgtatc
      661 tcttcctgct gaacctgctt cagatctaaa acaaggagta tatgcacagt atttaattgg
      721 aagctctgca acatctaata tttcattagc atgtatatta gtaacaggaa cttcagctgt
      781 tggtattaag aaatactcta atccttctat tttaaacata tcttcttcaa atttaggaag
      841 ttgaccagtc cctataaaac tatttctatt ggccataaat ggaggtaaga cttccgtata
      901 gccatgctta gctgtatgag tatttaaaaa gaaattcatt aaagatcttt ctaatctagc
      961 tcctaatccc ctatataaag taaaccttga cccagttatt ttccctgctc tttcaaaatc
     1021 taaaatacct agacctgtac ctatatccca atgcgcttta tgctcaaaat caaatttagt
     1081 aggttctccc caagttctta tttgaacatt gtcttcatct gtttcacctt gtggtacctc
     1141 tggatgagga acatttggta ttctcattaa tgtatattcc atttttgctt ctacttcttt
     1201 tactttctca tcaatacctt ttatcttatc tgataactct ttcaatcttg ccttttcttc
     1261 tgttacatct tttccttctt ttattaattg aggtattttt ttagaattta catttaactc
     1321 atttttcata acttcaactt cttgaagtat ttctcttctt ttattatcta attccactac
     1381 agcatcaagg tcaaattctc tttcacctct tctttccata gcttttttga tatcatctag
     1441 attttctctt attcttttaa tatctaacat attaattcct cctaataatt ttgataatat
     1501 aagttataat tccaacttaa tttacaaaca ttatacttgt aaactcttag tttaaacaaa
     1561 ttttaaaaat aaaaaaagac ctccgtttac cctataaaag ggacgaaaat cttttatccg
     1621 cgttgccacc ctaattgact atactaaaat gtataatcct ctcagaccta taacgtaggt
     1681 aaacgtctag ctttaactag aagctctaga gtagattcaa aaaccattgt aatgagtttt
     1741 caccatccac tcattctctg aatacaatct gttttttact attttccttc acagcttttt
     1801 aactaattgt tttatttgat aatcattata ttatatatat taacatcttt caatactatt
     1861 attcaaattt ttttagaatt ttctttaatt ttttatatta aattcatttt ttctatttaa
     1921 ttaaaacctt atcttttatc cattgtattt agattacctg tattattaaa taataatgaa
     1981 gaaggaacaa atggaacttt tatatcattc tcatcaaatt tattttttac cttttccatt
     2041 acatcaaaat atacatccca gtaatcatca gtttttaccc aaggtctagc aactatttgc
     2101 atggtgttat ttcctataga gtttaaacct attataggct ttggatcatt taatattctt
     2161 ttttcgtttg taaaaatttc atttaatata gacttaacta catctatatc actatcatat
     2221 gaaatattat atgaaaaatc tattcttctt ttgttttgat gtgtgtaatt aattatatta
     2281 ttacttgtca gttgaaaatt aggtataatt atagttttat tatctggcgt cttaagaaca
     2341 gtgctaaata tttgtatgtc agaaactgta ccttctacac ctgaaccttg aatataatca
     2401 cctatattaa atggcttaaa aaataaaata atcataccag agcctaaatt tgataatatt
     2461 tctttaaaag ctaaacctat actaaatcca gcagctccta acacagctac aactgatgta
     2521 gtttgtatcc ctataatacc taagcttatc aatgatataa tagataacat agatatatat
     2581 attccatata caataaaatt atttacacct ttactcattc catatttgat tagaaacttt
     2641 gaaaccatcc ttcttactat tttagatata taaataccta tcacaaatac aattattgca
     2701 tatataatat ttggtatatt atcactaaaa ttatcgagca tattttctaa agttctctta
     2761 gttatattgc tatttgttga ggcagtctta gccaatgata aaaaattcat tcaactcctc
     2821 cttatgataa tattccgttg tcctttaact cttttactat agcttctata gtttcttctt
     2881 ttattgcctc aattgtatga atatggctac attctgtcag aaccgataat tgtttaaatt
     2941 catttgtctc aactttcttc ataaagttgt ctatatccat tttagaataa atattaagtt
     3001 ctgcagttat ctgaccatat gttggatgat ttacgatgac atcttttata ataccaccta
     3061 aatccacaat tatctttaat tcatcataaa tttctttaga atccttatga tttttacact
     3121 ttatttgttt tgtaaaatac ggttgattat atattatata tccttgaggt gtagctatta
     3181 tttcaaatcc tctagctctt ataagggcta tatcttgaac tattacttgc ctacttacat
     3241 ttaatagttc agctagtgtt ccacctttta caggatgctt agattctttt aaaatatcta
     3301 ttaatttact tcttctttca ttagaattca tattacacct tctttccaaa taaaaacttt
     3361 acctatacta tataatttta tattttatct tattataaat caatattgac tttatattct
     3421 atttaatctt aacttttata ataaataaaa aagac
//
LOCUS       DAELNY010000257         3452 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.260, whole genome shotgun sequence.
ACCESSION   DAELNY010000257 DAELNY010000000
VERSION     DAELNY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.260"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..1020)
                     /locus_tag="KPE55_003508"
     CDS             complement(46..1020)
                     /locus_tag="KPE55_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0494539.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(1060..1407)
                     /locus_tag="KPE55_003509"
     CDS             complement(1060..1407)
                     /locus_tag="KPE55_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494540.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1625..2581)
                     /locus_tag="KPE55_003510"
     CDS             complement(1625..2581)
                     /locus_tag="KPE55_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG0494541.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            3024..3392
                     /locus_tag="KPE55_003511"
     CDS             3024..3392
                     /locus_tag="KPE55_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG0494542.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1244 a          449 c          427 g         1332 t
ORIGIN      
        1 tttaattcaa agtataaaaa accttaaata attggattca actatttaag gtttttttga
       61 taattttaat attaaatttt ttatggattt tctgttactt ttatttattt taataacaga
      121 ttcaacaaaa ctctttttat ctcctaattt taagataaaa tcaggatatt cttcaaattc
      181 attgagtttt attaatttga aagaagaaga tagattttta aagttaataa ttttagagtt
      241 attcactaat acacttcttt ttttaaaata aatttctttt ttacttatat ttagtatagt
      301 taatatttgt ttaagatata ttattatatc ttctggagtt ctatcaaatt tgagttcaaa
      361 attttcatct tcaaagtagg gaatataata taaaggtgtt ttgagagcag gtaatttatt
      421 atataattct tcaagaatat cgatattttt aggggagtta aagaaaaagc atttacatac
      481 attcattaaa tccacagcat ctgaaagtga gttatgttta atatctcctt ctacagaaaa
      541 aatttttttc atattcagta aagatatttg cttagataag attctattat tgtatgtaat
      601 atgttgagat ataaaaggtt gaatgtcaat catcatatta aataactcta agcatttacc
      661 tgtatattta tacactctaa atgaagttag aaggccagtt atatcaaaat caccccaatt
      721 atatattgta acatcatcaa atttacaaaa ccatttataa aaatcatcca taacatataa
      781 aagattagga gcattattga tatctagttg gttcaatttg gttaaatttt tacatttatt
      841 tgaaagtatt ttattgtatt ttggcttaac taaagagtaa taattatcta ttgtattaaa
      901 ttcgttatca actaaaactc caccaataga aattaattcc gcaccattat attcttttgg
      961 tttattttta ttatcatcta gtatattaaa ttcaaagtct ataaataact tgtatttcat
     1021 aaatctaaag cacatcctca ggtaaattat ttattgagcc taagcatcat aagaatcaat
     1081 agatataatg actctaaaca tcaaaggttc attacaactt ttatttattt cataatttaa
     1141 ttcaaataaa tcttttaaga tataatttat aagtattctt tcccaaccca tttttaaatt
     1201 aactttagag tctattaata tattttctat ctttttaaat atttctatat ttgctggtat
     1261 tatactgagc tcaatattac ttttaaacgg gttagactgt aaatctgtta taagtatatt
     1321 ttttaattcc tctgtaagtt cagaaattaa actatcacct atgtattttt ttatgatttc
     1381 ctctctaatt tcttttaaat ttagcaatat tagtacctgc tacttacata gtagcttttc
     1441 ctttcctttt aatactatta ttcatttttg aatcttattt agatatattt taagattaaa
     1501 agtctttagc tatataattt tttacccatt tttaacttat attaacatat tacaggctta
     1561 tatctccacc ctgtttacat tgaggatgga gtatattgcc tattttgata aaaatgatag
     1621 gttgctattt gcctattcct ttttctgata atttttctaa catatctttg gtcattttat
     1681 ctaaatcata actaaactta aagttccatt cttttactgc actactactg tctagagagt
     1741 taggccaaga atcagctata ctttgtctta cagggtctac atcatattct atcacaaaat
     1801 caggaatata tttttttata gaatcagcta tttcttcagg agaaaagctc atagcagtaa
     1861 tattaaaagc atttctatgg atgagtttat cagctggagc ttcgattaaa tccactattg
     1921 attgaagtgc atcaggcata tacatcatat ccatttttgt accttctgct atgtaagatt
     1981 tatatctctt attttttaat gcctcataat atatatcaac tgcataatct gtagttcctc
     2041 caccaggagg cgtaacataa gaaataagac ctggaaatct aacacctcta gtgtctactc
     2101 caaattttga gtgataataa tcacatagta attctccaga aactttagtc acaccataca
     2161 tagtattagg tctttgtaat gtatcttgag gagtcatatt ttttggagaa ttttcaccaa
     2221 aagcagcaat tgaactaggt gtaaaaaatt tacaatccaa ttctttggct atttcaagac
     2281 catttattaa tccattcata tttaagttcc aagcttctag aggtttactt tcagcaactg
     2341 cagaaagtaa agatgctaga tgaattatag tatctacatc atattttttt gcaatttcaa
     2401 aaaaacgatt cttatctgta acatctaata tctcaaatat accagattca catactggat
     2461 taccttcttt tactctcctt gaactagcga ttacattttg ttctccatac tcatttctaa
     2521 gctttattgt taattctgaa cctatttgtc ctaatgcacc tgttataagt atttttttca
     2581 attttacaac ctcctaaggg tttaatttat gtagtactac ttatttttat tttagcaaat
     2641 cttttgttta aatacaaaga attaaagata ataatatacg acttaattga atgaagtgca
     2701 gtaagtgcgt aaaaatttaa agctaatata taatggtcta ttgtataaca ataaaagagt
     2761 agattaaatt attatatata gattgtgtga ctaagtcaca gaaaatagag ctaatttaat
     2821 atataataaa agcatgtcaa gtaaagcttt aaattaaagt aactttatat aaaaagtaat
     2881 ataaattgat aaaattataa atttatttat agtgaatata ggttggattt tatagataca
     2941 aaaatagtat atatgatatg tagaccatta gcatgtttaa agtagcaaaa catataaaat
     3001 aaaatttgaa aggaagtata aaaatgacaa gaccatcaat atatcatagc caaggataca
     3061 cttgtgcaga ggcattaata aagtcttata atgaggaaca caacacagat ataccaatct
     3121 caataggaag tggtatgggg gttggaatga atgttggaag tgtttgtgga gctgtaaatg
     3181 ctgctgttgt tataataggg tatttaaatg ggcgaaatag taatttagat gaaaatatag
     3241 caaggaagta ttcaagagag ctattgaata gagtaagaaa taaatacagc tcagaaatat
     3301 gtgtatctct taaaaaaaat aaagtaactt gtgaagaaat aataaatttc acatatgatg
     3361 ctctaaatga tatgctaaaa aatcaaaaat aattctaaga aacaagtatt ttacattcga
     3421 tagaatactt gtttttttat gatattttta at
//
LOCUS       DAELNY010000258         3387 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.261, whole genome shotgun sequence.
ACCESSION   DAELNY010000258 DAELNY010000000
VERSION     DAELNY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.261"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..622
                     /locus_tag="KPE55_003512"
     CDS             <1..622
                     /locus_tag="KPE55_003512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494543.1"
                     /translation="RINESMVKKNETVGKDTYLSNIMKQKQELNDRIRDLKYRQEVYT
                     KKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEDKNKSTMLDENSTYLNTNDEKES
                     LITKESNEKIEEEILNDEKLEELEQKKDSNKKEKVSEDLSLVGKTREELENMLKNFIN
                     LTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTDEQKKHINVLI"
     gene            800..1690
                     /locus_tag="KPE55_003513"
     CDS             800..1690
                     /locus_tag="KPE55_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494544.1"
                     /translation="MKKNILTTVITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGDNYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2092..3048)
                     /locus_tag="KPE55_003514"
     CDS             complement(2092..3048)
                     /locus_tag="KPE55_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0494545.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKKMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
BASE COUNT         1320 a          391 c          506 g         1170 t
ORIGIN      
        1 aagaataaat gaaagtatgg ttaagaaaaa tgaaactgta ggaaaagaca cttatttatc
       61 taatattatg aaacaaaagc aagaacttaa tgatagaatt agagatttaa aatatagaca
      121 agaggtttat actaaaaaaa taaatgacgc aattaagaac ttatgtaaat cagaaataag
      181 agaaacaact aataactttt ctaatataga aataggtatt aaaaatagca ttatagaaga
      241 taaaaataaa agtacaatgt tagatgaaaa ttcaacttat ctaaatacaa atgatgaaaa
      301 agaatcttta atcactaaag agtctaatga aaaaattgaa gaagaaatat taaatgatga
      361 aaaattagaa gagttagaac aaaaaaagga ttctaataaa aaagagaaag tatctgagga
      421 cttatcttta gtaggtaaaa ctcgtgaaga gcttgaaaat atgcttaaaa attttataaa
      481 tttaacacaa gaagaaataa tgaaacttga gtcgagaata gaaaagttag ataaaaatgc
      541 tgaagaatac aaacaaaatt caaagactaa tatatttgat aaaacagatg aacaaaaaaa
      601 acatataaat gtactgattt aaacaatatg ttttaaaaaa ataagtttat tatataatga
      661 aaatataaag gtacaatttc aatacacttc tattaaacca ataatttatg aatcagactt
      721 aatttcaatt tactaataac catatattta tgagttaata attttatgtt ttaaaaagaa
      781 ttaaataagg gggagtagta tgaagaagaa tatattaact actgtgatta ccatgattat
      841 tatattgatg atgagtatga cggtaatggc agagccatat caagaagatg aaacatctag
      901 cttgaaaaaa cagagtgcaa atgaacgtaa tcagactaat ataggcacaa tggatggcag
      961 agtgcaaaca aatgcaataa gtaaaggtga taattatgac cctaaatatc ctcttaaagg
     1021 aatgttaaca caacttggtc ttaatacaac aggtaaagag ggatgggatt tactctatgg
     1081 aggaaatccc aatggagttg agcttcgagc agatggtaaa tattattata taaataaggg
     1141 tatggaaggg ctaggatttt atgcggcagc attgtcaggt ggctcatatc agagaatagg
     1201 ctttggaaaa cttgacccta tagaagtaat agctgatgga aattggattt cttacaaaca
     1261 agctcaagat acacttacag taataaggaa ttttttaaat agttttgact ggagaaatgc
     1321 atctgagatg gaaagagcta atagagctgc aaaattggta accgaagcaa aatatgcaga
     1381 ctctaaatat tgtaacatag tctatggtaa tctggttgat aaacgtggtg tatgtggaag
     1441 ttttgcaagt agctttcatt tattaacaag gttaatggga atggattcac tatctatatt
     1501 aaatccatca ttaaatcatg cttggaacta tatacaaatt gatgggaaat ggtataggtt
     1561 tgatgggtct gaaatatcag cttttggtgg atcattagac tttgattacc gtaaattaaa
     1621 ggatgcaaca agagagatga ctacttacta tgatgcaaag gcattgtcta tattaggatt
     1681 taatcaataa aaataggggg aggcttctta tagagccttt ttttattgat ttgaaattta
     1741 ataaggaatt ttatatgaaa atttaatcaa taaaagcaaa aaaggctctt tataaagcct
     1801 ttctaagtgt ttttttaagt aaaatttttt atatcaacat cagttttaat caaaacatat
     1861 aacattttgg acttactttt aaaaataaaa ttattaaaat ataatattaa tcatgttaat
     1921 aatgttttta gtttattata ttctataata gtggtcatgt tgactattgt agttttatta
     1981 agaatagttg gtttatataa atatatgcaa agatagcttg ttgtttactt tacttattag
     2041 tatataggac aagatactct aataaatttg cctattttaa tatcttttgt actaaaacca
     2101 actaaagttt cctttattat ttcgttttga ataatacaaa gcataatccg attgtttgta
     2161 taaagactgg aaagtatcag agttattttc taatttagat atacctatag aacaagaatt
     2221 actctctagg cataattcta aaaccttctt tgccttattt tcaacattct cttttgattg
     2281 attactatat tcaaaaacag caaattcatc tccaccaagt cttccaataa tagcatcatt
     2341 tacgaatact ttagtcaaaa tatttgaaaa atgtattagt atttcatctc ctcgaacatg
     2401 accaaagata tcatttacct gtttaaaatt atctaaatca aagatgtaaa agtatccatg
     2461 tgaaacagtt ttaagcattt tttttatgtg tacttcaaaa aaatgtctat tgtatagtcc
     2521 agtcataagg tctttttctg attttagttt aaattcattt gccattgcta gtgcttcttg
     2581 tgctttttca gttattgttt tagggtaata ctcatctttc atttggtcaa agtttggaat
     2641 agaatggtga acatggataa gtttaatcat atcatctgta actctgtata ccatagtaaa
     2701 tcgagtatcc atttctacat agatatcttt ttctaggtaa tctttttcta ttacccaaag
     2761 agtaccatat actataaaag cttcgtctgt gactttggta catgaatacc actcatctat
     2821 aatctcaaag tctatttgct ctgattctgt ttggtctttt tcaagaccta taataaattc
     2881 ttctaatgtt tcgtaaaatt catgttttcc tgttccaatt aatgcaaggt tgtcatctaa
     2941 taaggaaata ataaatttaa ggttttcttc ttctggattt aaaatataaa tatgccaaat
     3001 tttttttgat aaatctctta attgtttttg tattgtatta ttttccatag attaccccct
     3061 taagatttac agtttatatt ttaatgtatt ataagttgaa ctcttattac aatattatta
     3121 taatatagaa ctaaagaaat tacatcctgt ataggtaaaa gtatatcttt tacaataaaa
     3181 atattacagt aaaatgacca gttttaatat acgtattata tttttaattt ataaacgaat
     3241 attataaatg aaatttaaat tcaaccttga agaaataagc taaatgtatt agtatataaa
     3301 tccatgcttg aacataaata acctttatga tatgatatca ataataatat tatgagagga
     3361 gtgccttgat gtcggagtta tataatg
//
LOCUS       DAELNY010000259         3387 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.262, whole genome shotgun sequence.
ACCESSION   DAELNY010000259 DAELNY010000000
VERSION     DAELNY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.262"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(65..2284)
                     /locus_tag="KPE55_003515"
     CDS             complement(65..2284)
                     /locus_tag="KPE55_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0494546.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            complement(2277..>3387)
                     /locus_tag="KPE55_003516"
     CDS             complement(2277..>3387)
                     /locus_tag="KPE55_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494547.1"
                     /translation="EKIISKIKPKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEML
                     DLISEKVISLIEEDVSPRDIAIISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDY
                     PYGNALVVATCIFYGYLDFIKDDEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEH
                     SQYREILQYIEEKKKSDIKIHEFLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSES
                     LSLLGMKKEKIFIEALKSTINDYYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIW
                     VDIGSNAWNMKIEKDISNLIVLRKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKK
                     IYAFKSEYAINGYIQESILYSILLKISHKGDKNYD"
BASE COUNT         1042 a          586 c          288 g         1471 t
ORIGIN      
        1 tgtacatatt gttttatccc cttttagata tatttcaagc aatcttttaa taacttatta
       61 tatattaatt tgaccttttt tcaataatat gttgattaag tctgcttaaa tatatacatg
      121 gtttttgctc ttttctctta ttccctatat cactttcatc tctataagca cttcctgaaa
      181 gtactaacat ttcctttgct ctagtaatcc ctacatataa aagtctaatc ttttcattta
      241 tagaatcaat cttcgtttta tgagcataat ctgttgaaat acttcctttt aatatagctt
      301 caacttctga tttaataatt gccatagggt ttttatattt ttcttttaga taccatttat
      361 cactctgaaa cttttgatta atattgtcag ggaaattata ttcaacaaga cctagtaaaa
      421 acacacaatc ccattccatc ccctttgact tatgatagtt acatagtgtt atactaccgg
      481 gctctggttc atatccattt atctcatata ccacatctat tatatgatta aacaccctgt
      541 tctttacatc aaacaataaa ttatatacat cttctaaatc catatttata ttttctgata
      601 ctagatactt tatataaaaa gatatataat ccagtacagc tttatcttct ttttctaaat
      661 taagtttttt cccaataaac aaaactaaga ggtctaacct tattattgga tattctaata
      721 tctgttttat aatatctatt cctcttttaa aacttaagta tatatcactt tcttctttaa
      781 attctattaa atcattatct aacttactaa attcaaaatt ataaataatt tcttcggtag
      841 aatagttttt taattcttct aaaaaatact ttatttcttc ttcattatca gtatctataa
      901 aaactttatt aagtacaact attaactttt ctacatcatc acaatgcaac ataaaatcaa
      961 taatatatgc tatattgttt ataaccttcc ttttattcaa agaatttggt cctaattctt
     1021 caaaggttag attattctct ctaagttctt tagcaacttg tgtcacttgt tcattaaatg
     1081 gaaccaatat acctatactt ttatctggat atttattttt tataccctta acgtatctag
     1141 cagtttgctc tatttcaatt ttccaactct cataccactt tacatttata ttataatgtt
     1201 ctggattagg gttttcttta tatcccattc catttggcac agtttttatt tgcatatctt
     1261 caagagcatg tctacattct ttctgtctaa attcctttgt aacatacttg acaagagtgt
     1321 ttgctaaatc aagtatatct tttgaacttc gatttgacat atccatccta taacagaaat
     1381 ctgcactatt tataaattct ttaaaaaatt tagggtctga tgatgaaaat gttcctgtta
     1441 tactctgatt tatatctcca actctaacta ggtttttatt ttcgctagat ataattttta
     1501 ttatattacc ttgaatttca tttgagtcct ggcactcatc ttcaaatata tatttgtatc
     1561 tagtttgaaa cttctttcta agcccttcgt ctaagcttaa agttttttct gctaaaatta
     1621 atatatcatc ataatctagc aatccatttt gctttaattt tctatcatac aatttatata
     1681 ttggtaaaat aattttaagc attcctttgt gtcctcttga caatatttct tcaagtttat
     1741 ctggagaaat tcccttatac ttaagttcac ttattgcatt tcctgcaaat tcaaaaaaac
     1801 ctctttccca ggcttcaaga gttatttctt tccagcgttc atctttttgc tcctttaaaa
     1861 accatctaaa agctctttct ccaccttcaa atctaaatct acttatacat tcatttaata
     1921 taatactttt ttgcaaatcg tcagctatgt taaaatcctc acttaacatt acaacttctg
     1981 gcttttcctt tattattttt acagccaaac tgtgtatagt cattacttca tacgaattag
     2041 tatcatctat tttattctct tcaagaatct ttcttattct acctttaaaa ttatttacag
     2101 cactattcat atatgtaaga attaatattt taccaccctt atgtttctgc tcaagtaata
     2161 gtttagctac taaatttgtt attataaatg tttttccagc acctggtact gctggtactg
     2221 ccatagtacc attatcgtaa tttattattg gtatctggtc ttctctataa tttatattaa
     2281 tcataatttt tatctccttt atgtgagatt tttaaaagta tactatataa tatactctct
     2341 tggatataac cattaatcgc atattcactt ttaaatgcat agattttctt tgcatttaat
     2401 aacaaattat atactgtatt gtatagatag tattttttat aatattcttc catttcattt
     2461 gtataaatct ttttttcttc aaaagatttt ctaagtacaa ttaaattact tatatctttt
     2521 tctattttca tattccaagc attacttcct atatctaccc atatttgtat acttctatct
     2581 atatttgatg atatatatga ataaggagta gttattacta ttttgtcttt ggatttcaac
     2641 tcttctatat cgacaacaga gtaataatcg tttattgtac tttttaaagc ttctataaat
     2701 atcttttctt ttttcatccc aagtagactt aaactttcac taaaagactc actttcagct
     2761 attatctttt tacataaccc aacattcttt ttaccttctt ttaaatttaa cattttatct
     2821 atgtaaaatt gagttaaaaa ttcatgtatt tttatatcac tcttcttttt ttcttcaata
     2881 tactgtaaaa tctcacgata ctgactatgt tcatctatat tatcaatact taaatgtctt
     2941 gtttcattaa aaatcttaaa tgcttttatc ctatttattt ctaaaagagt ttctaaaaag
     3001 cttaagtatt catcatcttt tataaaatct aaatatccat aaaatataca tgtagccact
     3061 accaatgcat tcccatatgg ataatccaca gcttttttat ccttcttagt actaaaaaca
     3121 tctatatttt tttctatcaa agaatctcta atttgatgct ccaatatact attgtttata
     3181 ggagatataa ttgctatatc tcttggagat acatcttctt ctataagaga tataactttt
     3241 tcacttatta aatctaacat ttcattatat aattggcttg attgatttaa ttctatattt
     3301 gcaggtaata agtataaatc ttctattcta atctccttct ttttaatatt ttcttcttta
     3361 ggcttaattt ttgatattat tttctca
//
LOCUS       DAELNY010000260         3340 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.263, whole genome shotgun sequence.
ACCESSION   DAELNY010000260 DAELNY010000000
VERSION     DAELNY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.263"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..967
                     /locus_tag="KPE55_003517"
     CDS             <1..967
                     /locus_tag="KPE55_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0494548.1"
                     /translation="NNQYKGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVES
                     INSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFY
                     TWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRT
                     NWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSST
                     VNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCF
                     EAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1021..2145
                     /gene="wecB"
                     /locus_tag="KPE55_003518"
     CDS             1021..2145
                     /gene="wecB"
                     /locus_tag="KPE55_003518"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG0494549.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2223..3197
                     /locus_tag="KPE55_003519"
     CDS             2223..3197
                     /locus_tag="KPE55_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0494550.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1398 a          330 c          588 g         1024 t
ORIGIN      
        1 aaataatcaa tataaaggta agattaaatc ggccaattta tctgtagatt atgaaataaa
       61 tcaagttatg aaggatatag ataaattggg attgaataca gtaaatgtac ctataattat
      121 aaatgtagag tccattaatt ctgatattat gtcaatagat aataatagta aagaaaaggc
      181 aattaagtta ataaaaaaat taaataaaaa aggtatttca acaatattag aggcatatcc
      241 atggattgaa aatggaaaat tgtatgagac tgactggaac cctataaata aaaaaagatt
      301 tttttatact tggcaaaatg ccatattaaa tgaacttata attgatgtag caaatccact
      361 ggatgtaaat gttttaaata ttggttctaa ttttgtacat ttggaagaat atcaacaaaa
      421 ttggggtgaa ataatagatt ttgtacaatc caaatttgac ggtcttgtaa cgtacagaac
      481 taattggtgg tatacaaaaa aagatgattt aagtagtaag ttgttttatg aacaaaagct
      541 gaacaataat ttttttgata agttagattt tatatctata gcagcatatt ttgagttaag
      601 taataaacct gttaatacag tggatgaatt aatttctgcc ttacactcat ctactgtaaa
      661 caatagagga caaaatataa aacaagaaat atataacctt tataaagctc atagaaaacc
      721 tatatttttt ggagaattag gtttttcaaa tagagaaagt gcaagttctc agccgtggaa
      781 tcatactcct tcaaaagctg taaatggcga agaacaagca aggtgttttg aggcatataa
      841 gaaagttttt gaaaatgaag actggataaa tggtttttct gtgttttgtg taggtaaaat
      901 agatgacgaa aaaaattttt acccatcaaa agagagtata aaagttatta agtcttggta
      961 tgaataattt tgaaagtgta gaatttatat agattaaata gatgattagg agagatataa
     1021 atgaatgaca tcaaggttat gactgtattt ggaactagac ctgaagctat caaagtagca
     1081 cctttaataa aggaacttga aaaaagagag aatataaaaa gtattgtttg cgttacagct
     1141 caacatagag aaatgcttga ccaagttata gaaaccttta atataaatgt agactatgat
     1201 ttggatataa tggaaaaagg acaaagcctg aatgatataa catgtaagat tttaaataaa
     1261 cttcccttga ttttaaacaa ggaaaatcca aatataatat tggtacatgg agatacaacc
     1321 acaactctag ctacaagttt gactgcattt tataataaaa ctcttgttgg tcatatagaa
     1381 gctgggctta gaacctatga taaatattct ccttttccag aggagctaaa tagacaactt
     1441 acaggtatta ttgctgatat gcactttgct cctactaatt tggctagaaa aaacttgatt
     1501 tctgaaggaa aacctaataa taatatattt gtaactggta atactgctat tgatgcttta
     1561 aagatgacaa taaaagaaaa ctataatcat cctataattg atgagattgg taacgatagg
     1621 atgattttgt taacctctca cagaagagaa aatttgggta aacctatgaa aaatatattc
     1681 agagctataa agcgaatagt agatgacttt gaagatgtac agatagtata tcccatacat
     1741 ttaaatccta aaatacgaac tatagctgat gagatatttg gaaaatttcc tgaaaaaatt
     1801 catattatag agcctttaga tgtagctgat tttcataact ttttaaataa atcttatatg
     1861 ataatgactg atagtggagg aatacaggaa gaagctccat cattaggtaa gccagttctt
     1921 gtgttgcgtg ataaaacaga aagaacagaa ggaattgaag caaagacact taaattagta
     1981 ggtactaatg aagatagaat atataattca gtgagcgatt tattaataaa taaagataac
     2041 tatgttcaga tgagtaaagc atctaatcca tatggagatg gaaatgcaag caaatatata
     2101 gtggatataa taattaaaaa atttaattgc aaatatttaa attagataag gcctaaaaca
     2161 ttaactaatt gatataagat ttatgataat attaagtaat acaattttta agggggagta
     2221 atatggctag aaaattaata aaaaatttgg gtaaaagtaa aagtgtaaag agagtgaaac
     2281 ttttatttaa gaagattttt attactgtat tcatagtagc aagtatagtt gctattttta
     2341 atataacgaa atattttgag gaattataca aagtaaggga tttaaaaagt actaaaattg
     2401 aatactatat ggatgtggct gatgaagcag gagatggaaa ggtccaatta agttggaagg
     2461 ccttacttgc tatagacatg gtaatacatg atgaagattt aagtaatata aaaaagaaag
     2521 acacattgga tataggggaa aagtttatag tagaagataa aaatggtaaa ggcgaaaagg
     2581 tgtataaggt aaaaaagttt aataaggtat taagcgaatt gaaatttgac tcttctcaaa
     2641 aaagtagagc aagaaaatac atgaaagatt tagaatacac ataccttgga aataaacaat
     2701 tagatagtag tgatgaaaaa attaaattta taaagaagtt agaagactca gctataagag
     2761 aatatattga ttatggaata ttgccctcta taacaattgg acaagctata ttagaatctg
     2821 gttggggaaa ttctaaactt acaaaacaga gtaataattt atttggtata aaagcagata
     2881 aagcatggaa aggaaaaagt gtagaaattt caacttcaga gcattataat gaaaaaattg
     2941 tagctagttt taggtcgtat aattcattac aagattctgt caaagaccac agtttatttt
     3001 taattaataa taaaagatat agaaaacatg ggttgtttga ggcaaaagat tatattagtc
     3061 aagctcaagc attagaaaat gcaggatata gtacagcaga agataaaaaa ggaaatcgta
     3121 tatatgcaga gctattaata gatgttatta ggagttataa tttgcaattg atagataata
     3181 aagttgagac aaaataattg ttaactttat atgaggagat aacaagataa gatttttttc
     3241 tacaaaaaga atagaaaaat tgtaaaaaaa actaaaaaaa tgtattgact tgttatttaa
     3301 aaggtgatat tattattact gtactcgaaa gagaacaaaa
//
LOCUS       DAELNY010000261         3324 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.264, whole genome shotgun sequence.
ACCESSION   DAELNY010000261 DAELNY010000000
VERSION     DAELNY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.264"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..785)
                     /locus_tag="KPE55_003520"
     CDS             complement(<1..785)
                     /locus_tag="KPE55_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494551.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITA"
     gene            complement(1594..2631)
                     /locus_tag="KPE55_003521"
     CDS             complement(1594..2631)
                     /locus_tag="KPE55_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0494552.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2728..>3324)
                     /locus_tag="KPE55_003522"
     CDS             complement(2728..>3324)
                     /locus_tag="KPE55_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0494553.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT         1141 a          511 c          444 g         1228 t
ORIGIN      
        1 atggcagtta ttagttttaa gtcagttgat aacattccta aactcattgc aaagtatata
       61 gtgaattggt atataaaaga gccaacaatt attgcagtag tatctttgat aaaagtaaat
      121 ttcttaaata gtgtaatacc aattatgata gaggcaattc caagaactaa agttcctata
      181 cccatattta catctgagaa tcccaagaac tgagccataa gactaccaga taaagctata
      241 agtccgtttg aaatcataag tcctagtatt tttatagaac cgatatttat acctagggat
      301 ttaatcattt gagagttatc accaacacct tttaaagtgt atccaagacc tgtttttagg
      361 aataaatcta aaaggacttt acagatgaat acaaaagcta aggctaaaac tataggagat
      421 atttccccat tgaataagtg tttaaagcta aatagaggaa tatttgattt tcccattatt
      481 cttaaattga tagagtatag cattcccata actaaaatac cagaaagtaa gttagatatc
      541 ttaaacttaa tgtgaagaat ccctgtaaca agaccagcag tacaaccaca taagatagcc
      601 attaaagttg ctactacagg agaaatacca ttagtaagag aaaatgctac aatagaagca
      661 cccattgtgt agctaccatc agcagacata tctggaaaat ctaagatttt gtaagttata
      721 taaaccccca gtgccataat cgataaaatt aaactttgcg tcattacaga tataatacct
      781 gacataaata aaacactcct ttgaaaatat ttttataatt ttaattattt gctaattttg
      841 tgtataaaaa aaccacatag tgtacaaaac tatgtggtaa aaacacgaat aagccacata
      901 gtttaatcta tgtggctctt atatagttaa atgaaaactt tataaacaat atagccaaca
      961 tagatacaat cccaaaattt tagtagtctt aaggggtctg tatctatgtt aaaacgtagg
     1021 tacaaaccct aaaaatagtg gctataataa taataatatt ttctattgtt taataaagta
     1081 ttcataatta tctctccctt ttaatatttt aattagtcag aaaattatat gtattctcga
     1141 cttgttataa attataaatt atacaataat aaaagtcaac tgctaaattt aattaaaata
     1201 ctaaataatg tatactcaga atacttttaa attaatgtat tgtttgcaaa aaacgttatc
     1261 ctaagttata agtgaatgtg gaaattttat caaaatattt actagatatg gaataactag
     1321 acatgtatat caatcaaata tcataaaaaa aagctgtctg gagaatatta aaattctttt
     1381 ggacagctca agtagtgatt agaaagttat ataactttca taatcagtcg tcaatataga
     1441 cgacttttat ggaaaatgaa taactttata tgcaatattc aaattgctac aataagcaca
     1501 attttcaaag ggttagggtg gataagagta cttattatat aaagttttca tattacctac
     1561 ttcatattac ctattttttg aaattaatag gttttatttt gcagattttg cattttcatc
     1621 tgactttata tactttatat tatctgctga taacttttgc atgtcaagtg cttttagtgt
     1681 atcttcgtta attattattt cagtttcatc taatgtagta actggtatat cgccaacaga
     1741 ttttccttct aatacttcaa tagcaagttc tccagcctta taacctagtt tttcataatc
     1801 tattccacaa caagctaaag caccagaaga cacagaacct tcttcagagg ctattatagg
     1861 aatcttgttt tcattagcaa ctttagaaac tattggcata gaagaaacta ttaaattgtc
     1921 agtagggaca tataaaacat ctattttgcc aactaaacta gaaatagctt ggttaacttc
     1981 acttgaagag ctgattcctt tttcaactac ttcataatta tttttcttag cataatcatg
     2041 tagagaatca acttggattt ttgaattaac ttcactagta ttgtatataa cccctatttt
     2101 ctttgctttt ggagtcaatg ttttaaccaa ttctaatgtt ttatcaattg aaagataatc
     2161 agatgtacca gaaacatttc cacctggttt ttcaagagat ttaacaagtc cagcttctac
     2221 agggtctgta acagcagtca ttattatcgg aatatcttta gtagcattat aagctgcttg
     2281 tgcagatgga gtagatatag catatatcaa atcttttttg tcggatacaa acttactagc
     2341 aatactttgt gtagtaggca tatcattttg tgcattttgg aaatctattt ttatattatc
     2401 tccatcttta tagcctttat cttcgagtgc tttgatgaat cctttctttg ctttatccag
     2461 agatggatgt tccactaatt gagtaatacc tatatttttt tgttttttgc tatctgtttc
     2521 tttattttca tttgaagcat tggagccatc attttgagaa catccagtaa gcattgatac
     2581 accaagtact cctgctataa ttaaacttaa tattttttta ctttttatca ttttattttc
     2641 ctcccttaaa atttattaat tatatttgtt catatattca tatgatagat tataaatatt
     2701 tctgattaaa actaagattt taatttctta tactgcatat tcaaattttt ctaagatttc
     2761 ttcaactgtt aaatcttctt tttctttatc ttttacatct aatacaactt cacccttatg
     2821 aagcattatc aacctatctc catattgaag agcgtgtttc aagttgtgag taaccataag
     2881 tgtagttata tttttctctc ttacgatttt atctgttaac tctattactt catttgatgt
     2941 ttttgggtca agtgctgctg tatgttcgtc tagtaataag accttaggac ttgctaagct
     3001 agacataatt agtgatagtg attgtctttg accccctgaa agatattgta ctttaatatc
     3061 taaatatttt tttaaatcca aagaaattcc ttcaagtaag tgttccaagt cattcgtttt
     3121 atgacgaaga cattttttta agttcaataa tttcccttta ttaagtgcta gagataaatt
     3181 ttctctaacc gtcatagaag gacaagttcc aaggcttggg tcttgaaaaa ctctacttac
     3241 tatttgagtt cttttatact cagctaaatt tgttatagta gtactatcta aagtaatttg
     3301 accagaagat tcttttataa gccc
//
LOCUS       DAELNY010000262         3289 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.265, whole genome shotgun sequence.
ACCESSION   DAELNY010000262 DAELNY010000000
VERSION     DAELNY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.265"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..811
                     /locus_tag="KPE55_003523"
     CDS             <1..811
                     /locus_tag="KPE55_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0494554.1"
                     /translation="LVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLAGISAK
                     QANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLSIGRVC
                     KFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRKGNNRN
                     MVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNNFMNIF
                     VSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            940..2205
                     /gene="gluD"
                     /locus_tag="KPE55_003524"
     CDS             940..2205
                     /gene="gluD"
                     /locus_tag="KPE55_003524"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG0494555.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            2418..3008
                     /locus_tag="KPE55_003525"
     CDS             2418..3008
                     /locus_tag="KPE55_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG0494556.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCEFYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFVAGLNKQSDIIKRVMNMFSEKHKNGI
                     LMALIISLFSSITFFKFDSTSIIEENDFLERLRYTFEGRQEDMFVTIDFTYADTPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         1315 a          318 c          607 g         1049 t
ORIGIN      
        1 tttggttggg tcaagtggca cagggaagag ctttaaggct ttagagttcg catatgaaaa
       61 tgatatagaa tatataattg atgatgggat tttaatatac aaaaacaagg ttttagctgg
      121 gatatcggca aaacaagcaa atactactat agaagcagtt aaaagggcta ttttttataa
      181 tctggagcat agacaagaag ttagagaagc aattaaaaaa gaaaatataa gaaaaattct
      241 tattattggg acatcaaaaa aaatggttaa tcaaattgta gagagattgt caattggaag
      301 ggtttgcaag tttataaata tacaagatat aagtactaaa tatgaaatag aaatagcaaa
      361 acaagctaga aaagaaggaa atcatgtaat tcctgttcca gcagttgaga taaaaagtat
      421 ggcaagtggt cttagtataa attctttgaa gagacttttt agaaaaggaa ataataggaa
      481 tatggtagtt ttagaaaaaa caattataag accaactttt agttatatag gaaagtttta
      541 tattagtgct gatgtaatta aacagattat tgaatatgaa gtatacaact ttggaagcat
      601 tgatagaata aataaaatca atatagagaa ttgcaataat tttatgaata tttttgtaag
      661 tataaatatc aatgatttag aaactataaa aaaaattgaa gacatacaac agacaataaa
      721 aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa atagatatta acattcataa
      781 aattaaaaaa ataaacttcc tccaatgtta aaaaataaac tgagaaaatg atatactaat
      841 ttgcaataaa gtgtgctata attaaactgt aaatttagga aatggttttt tctaaatgga
      901 gtatacataa ttataacgta tttataaggg ggctttcaaa tgtcaggaaa agatgtaaat
      961 gtcttcgaga tggcgcaatc tcaagtaaaa aatgcatgtg ataaattagg tatggaacca
     1021 gcagtttatg aattattaaa agaacctatg agagttatag aggtttcaat tccagtaaaa
     1081 atggatgatg gttctataaa aacttttaaa ggatttagat cacaacataa tgatgcagta
     1141 gggccaacaa aaggtggaat aagattccat caaaatgttt caagagacga agtaaaagct
     1201 ttatctatat ggatgacttt caagtgttct gtaacaggta taccatatgg tggaggtaaa
     1261 ggtggaataa tagtagatcc atcaacttta tctcaaggtg aattagaaag attaagtaga
     1321 ggatacatag atggaatata taaattaata ggtgagaaag ttgacgttcc tgcaccagac
     1381 gtaaatacta atggtcaaat aatgtcttgg atggttgatg agtacaataa attaactgga
     1441 caaagttcta taggtgttat aactggtaag ccagttgaat ttggtggttc tttaggaaga
     1501 acagctgcta ctggatttgg tgttgctgtt actgcaagag aagctgctgc taaattagga
     1561 atagatatga aaaaagctaa aatagctgtt caaggtatag gaaacgttgg ttcttataca
     1621 gttcttaact gtgaaaaact tggtggtact gttgtagcta tggctgaatg gtgtaaatca
     1681 gaaggttctt atgctatata caatgaaaat ggtttagatg gtcaagctat gttagattat
     1741 atgaaagaac atggtaactt attaaacttc ccaggagcta agagaatatc tttagaagag
     1801 ttctgggctt cagatgttga tatagtaata ccagctgcat tagaaaactc tataactaaa
     1861 gaagttgctg aatctataaa agctaaatta gtttgtgagg ctgctaatgg accaactact
     1921 ccagaggctg atgaagtatt tgctgaaaga ggaatagttc ttactccaga tatattaact
     1981 aatgctggtg gagttacagt ttcttacttc gagtgggtac aaaacttata tggatactac
     2041 tggtcagaag aagaagtaga gcaaaaagaa gaaatagcta tggttaaagc atttgaatct
     2101 atttggaaaa tcaaagaaga gtacaatgtt acaatgagag aagctgctta catgcattca
     2161 attaagaaag ttgctgaagc tatgaaatta agaggatggt actaagattt actcttaata
     2221 aataatagga tataattatt taagatataa aggggctgta catagtacag cccttaaatt
     2281 atgtggctta caatgaagta agctattttt gttttaactt ataaataact tgataaatta
     2341 tgtaatttag aatgtatttt ttaccaccac atgattacaa attagaggag ttagattgaa
     2401 tttttagaca tgaacttatg cattttaaaa gcaaagacct taatttaaag tatctagtat
     2461 tatttttaaa aatagtatat tggtttaatc catttatatg tataatggaa aaatatatgg
     2521 atttggattg tgaattttat tgtgatgaga gagttttaaa aaattgtact gtacaacaaa
     2581 agcaagaata tgctctaact attgtaaatt caatgaaaag aggtacaaat ttttcaaata
     2641 aatttgtagc tggattaaat aaacaatctg atattataaa aagagtaatg aacatgttca
     2701 gtgaaaaaca taaaaatggt atcttaatgg ctttgattat aagcttgttt tcttctatta
     2761 cattttttaa attcgattca actagtatta ttgaagagaa tgatttctta gagaggttaa
     2821 gatatacgtt tgaaggaagg caagaagata tgtttgtaac aatagatttt acatatgcag
     2881 atacacctat aaaacttaga aaagagcatg aagaaaactg tagagtcaat ggaatagtac
     2941 caaaagattc agattcaata gaaattattc ctagatttta taaagattgt atggatttaa
     3001 aaaaataagt tagacttaat attgatgttg atattatgaa ttttttatat ttaagcaatt
     3061 gaaaatttta ttatagattc aattttatat gtgtttaagt atgaaattta tagatagttg
     3121 aaataattaa atatgattga tgcataggaa tcattactaa gctataatta attatagggg
     3181 agtttaaata aattatgagt tacacaatta taaaatgata aaaatagtta tctgttacaa
     3241 aaaatgtatc tttttaatta ataaagataa tatgaaaact taaaaggca
//
LOCUS       DAELNY010000263         3260 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.266, whole genome shotgun sequence.
ACCESSION   DAELNY010000263 DAELNY010000000
VERSION     DAELNY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.266"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            535..729
                     /locus_tag="KPE55_003526"
     CDS             535..729
                     /locus_tag="KPE55_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG0494557.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(831..1340)
                     /gene="vanZ1"
                     /locus_tag="KPE55_003527"
     CDS             complement(831..1340)
                     /gene="vanZ1"
                     /locus_tag="KPE55_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG0494558.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1660..2049)
                     /locus_tag="KPE55_003528"
     CDS             complement(1660..2049)
                     /locus_tag="KPE55_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0494559.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            2442..2798
                     /locus_tag="KPE55_003529"
                     /pseudo
     CDS             2442..2798
                     /locus_tag="KPE55_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3108..>3260
                     /locus_tag="KPE55_003530"
     CDS             3108..>3260
                     /locus_tag="KPE55_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494560.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKR"
BASE COUNT         1213 a          402 c          339 g         1306 t
ORIGIN      
        1 cactactctt tatatgtaat tttctactat tatatattta tttactctat aaaataatcc
       61 ccattcaaaa cacttttctt aaatcactca aactaaaata catataagat tatcattagt
      121 cagtaagtat atatttttct aatttctaaa aatacatcca aatcatcaaa cgtttttagc
      181 aattacttta ttattataca gtgttaaagt aaagttccta aataaattgt tattcatata
      241 gcaaataaat acttggtatc aattttttaa tatctattta aatagtattt tttattttgt
      301 cactttatag atattaatgt gtatttaaca ctgctaaaaa ataataaaat tttataatct
      361 attcaataat atactttttt ctataaatat tttacccata acctttattt ttttattaaa
      421 atgcattata ataaaaagta aatctaaatt tgatacagtt ttaacaatca ttattttcag
      481 aaaagtgata ctattgaata tattaagaat aattagagat ggaggaacta agatatgagc
      541 gtaattgtaa tgtgtagtcg ttgtggaaaa gaaatagata aatatagaaa taaatatata
      601 acttattatg agaaaaatac caatgcagaa attaaagttt gtataaattg tgcgttagat
      661 ttattagaaa aaacaagaga aaatcaagaa ttgaacatac aagagtttca agatacaata
      721 attctttaat aatttaggaa agcacctgta taggtgcttt ttatgtgtaa ataattttat
      781 atgtaactgt atcttacgtt ctattttaag ctaaacatat atacttattt ctataagttt
      841 gcaaaaataa ctaatcctaa aaataataca acaataattg tcacaatcaa tgccaataca
      901 ttgataattt ttatggtatt attcttaaat attttcgata atagcataaa aacagctatt
      961 cctatgattc ctcctagtgt gtttcctatc aaatccgtta tatcacttgc acctagtgca
     1021 aatatatatt gaagtgtttc aaataccaaa ctagttataa aaataggtat aacttttttt
     1081 ataaagctcc attcttcttt tagcatacac acatatatcc caaatggtac aaatatagcc
     1141 acatttaata taatctctga tatatctact ctattattaa ttataagtga cccagcaaaa
     1201 ggtattaagt ttatgcttct aagattcatg gtttcaagag atgagatatc aaattgcaac
     1261 ttaaatagta ttatccatgt taaaataatt atgtaaacta taaataatcc ttttgtaata
     1321 ttatgttttc tagatttcat ttattacctc cttactatat tattgtatta ttttattaat
     1381 acaataatat agtaaggtaa tttatctgtc aactatttct tctaaaatta tattcctttt
     1441 ataatttcaa tatctttatc atatttacat gatttaagct attgtagtat ttcatatatc
     1501 tttaaatttt aattggtatt attctctata ttttttacta tcaaacacta aggaaataat
     1561 cacttttctt tatataatgt tctaccatca cataaaacta acattaaatc caaacgctac
     1621 tctcaattgt ctcaaactaa aaagatactg atagctttat tagtcatcaa tatcttcttc
     1681 tttcaaattt ttaaagtatt cttctaattt gtccaagctc ttactatcta aaacttcatc
     1741 atcatgtagt gctgaaataa aactcttaaa tgaattgtta tgcataaaac taaaaaaact
     1801 ctttgtctca acttttaagt aatctttctc ttttatagct atagtataat gcgtacatct
     1861 atctacctta tcagtagtta aaaagccctt atctactaaa ttttttaaaa atgtcaatgt
     1921 tgtgctcttt ttccaatcgt atttcaactt catgtcttct atgatatctc ttgaggctaa
     1981 tacatcatct actccccaaa tatacttcat tagttttagt tctgcatgtg gtaatttatt
     2041 aatcttcatt agtttacacc tcttaccaat ttatgtataa ctaaacttta taacaataag
     2101 tgtatgtaat caattagatt ggaataaata taaattccaa tttattctat ataaaaataa
     2161 ttaataaaaa agaagtactc tactttagag agtacttttg gattatatat tcaaaaattt
     2221 atctaacatg attagagtat gttttatgct ttagtgtgat aattttagtt cgttttatta
     2281 ttgcaacatg tattatattt cattataatc tataagctta aatacaggat atttatttca
     2341 tatacttaat ttatattaag ttttattata tgtattatta agttttacta tattttttat
     2401 taatatttaa tatagttaat ttttaataat tatattatac aataacaata tataagctaa
     2461 ttaaaaacct taagaagaac gatattgaca ctacttgaat tatattatag aaaagctgaa
     2521 ataaataaaa tagtttgggc tagaataggt tttaatttat atctttctaa aggagttgca
     2581 ttaactttaa ctgatgctgg tatagggatt tcaggacttt tggctggtgc tattcctggt
     2641 ataggacagt acttgccact tatagttaga ttaattacaa tgtaccttga gtcaaatata
     2701 acatctggaa aaatctttaa atttaaaaaa tacgttataa taaaggaggt gcttgtgtaa
     2761 ccaaatacta cttacaatct gtagaaatca ataggtgata aaatgattat aaaatatcta
     2821 ctttttgcaa ttgtttcagc attaatttta ttattttgta ataaagcatt ccaactaagt
     2881 aaattacaca agttcattat attgtttgtt tcatttttta tactaacttt tgtagcatca
     2941 ttcattagta gataaaaaat aagatggtgg ggttaactta ccatcttttt ctcatttaat
     3001 atttgtaata ttttagatac tctttatact tgtgttactg ttatactttg attaggaaaa
     3061 ttaatacaac atgagttgat gattatatta taaaggaggt atcttctatg aattttagga
     3121 acatttatct tatacttatg ggattgggaa ttataatatt aactacaata atatctttaa
     3181 ttgaaattaa aattggtttt tgtaatgaaa aatattttaa taaattagaa tcaaaatatg
     3241 gaaatataga tagaaaaaga
//
LOCUS       DAELNY010000264         3234 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.267, whole genome shotgun sequence.
ACCESSION   DAELNY010000264 DAELNY010000000
VERSION     DAELNY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.267"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            318..2960
                     /gene="adhE"
                     /locus_tag="KPE55_003531"
                     /gene_synonym="adhC"
     CDS             318..2960
                     /gene="adhE"
                     /locus_tag="KPE55_003531"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0494561.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1250 a          404 c          620 g          960 t
ORIGIN      
        1 ttaataagta aaaaaaatga aagatataaa ttatcatttt agtattttat ttagtcgaag
       61 taaattattc aaatgaaata tatctcattt agataaaaaa ttttagtaaa agtaaaaaaa
      121 ataatatatt tgttaaaaaa taaattgaca aaatattcag aaaaaaactt cttgacctaa
      181 gagagtaaaa taaataaaat ctaaggtata gagatgaaat ctataaaatt attttaagta
      241 taagttaaaa atatagatga tgcatttaag atttataaaa attagagatg cataattatt
      301 ttagggaggt ttttagtatg gagaaaaaag aaaaggtagt agaaaaatca aatgttgaag
      361 tatgtagtcc tgaaattgtc aacagtgttg aaacattaag aatgagatta gaagaaatta
      421 gattggcaca aaaagaattt gcaacattta cgcaagaaca ggtagataag attttcttag
      481 ctgcttcaac tgcagctaat caacaaagaa tacctctagc aaaaatggca gtggaagaga
      541 ctggtatggg tattgttgaa gataaggtta taaaaaacca ttttgcctca gaatatatct
      601 ataatgctta taaagatact aaaacttgtg gtgtcataga aaaagatgaa gcatttggtt
      661 ttacaagaat tgctgagcca gtaggtgttt tatcagcagt tataccaaca actaatccta
      721 catcaactgc aatattcaaa tccctaatag cactaaaaac tagaaatggt ataattttct
      781 ctcctcatcc aagagctaaa aactgtacta tagaagcagc tagagttgta catgatgcag
      841 cagttaaggc aggagcacca aaaggactta taggttgggt agatgttcct tcgattgaat
      901 taactaatgt tgtcatggca gaggcagacc ttatattagc tactggtgga cctggaatgg
      961 ttaaatctgc atactcttca ggtaaaccag ctgtcggagt tgggcctgga aatgtacctg
     1021 caataataga tgagagtgca gatataaaaa tggctgtaag ttcaatctta gtatcaaaat
     1081 cattcgataa tggtatgata tgtgcttccg agcaagctgt aatagtacca gaaaaaatat
     1141 atgaagaagt taaaaaagaa tttaaatatc gtggagcaca tttcctaaat aaagaagaaa
     1201 ctgaaaaagt aggaaaagta gtaataattg atggttcatt aaatgccaga atagttggac
     1261 aaccagcaca tgtaatagcc aaaatggcgg atgtagaagt tccaaaaaca gctagaataa
     1321 taattggtga agtagaatct gttgaattaa atgaaccttt tgcacatgaa aaactttctc
     1381 ctgtacttgc aatgtacaag tctaaaagtt ttgaagatgc agttgctaaa gcagaaaaat
     1441 tagttgcaga tggaggctat ggtcatacat cttcattata tgcagactct ataaatcatc
     1501 cagagagagt agaaaagttt gtaaatgcta tgaagacttg tagagtatta gttaatactc
     1561 catcatcaca aggtggtata ggtgatttat ataactttaa attggcaccg tctcttactc
     1621 ttggttgtgg ttcttggggt ggaaactcag tatctgaaaa cgttggagta aaacatttgc
     1681 ttaatatcaa gacagtagct gaaaggagag agaatatgct ttggttcaga gcaccagaaa
     1741 aggtttattt caaaaaagga tgtttaggtg tagcagcaag agaatttaag gatgttatgg
     1801 ataaaaagaa agcatttata gtaacagatt ctttcctata taataatgga tatactaaga
     1861 aattaactga tttattagat gagatgggaa ttaagcatac tacattcttt gatgtagctc
     1921 cagacccaac attagcttgt gcaagagaag gagctaaagc tatggctgat ttccaaccag
     1981 atttaataat agctgtaggt ggaggaagtg caatggatgc tggtaagata atgtgggtaa
     2041 tgtatgagca tccagaagta gatttccaag acttagctat gagatttatg gatataagaa
     2101 aaagagtata tgtatttcct aagatgggtg aaaaagcgta ttttgctgct atcccaactt
     2161 cagcaggaac tggttcagaa gtaactccat ttgcagttat aactgaccaa gattcaggtg
     2221 taaagtaccc attagcagat tatgaattaa tgccaaacat ggctataatt gatgctgata
     2281 tgatgatgga gatgccacca agattaacag cagcatcagg tgttgatgct ttaacacatg
     2341 cattagaagc atatgtatct atgttaagaa ctgaaccagc tgacggtcta gcactacaag
     2401 caggtaaaat aatatttgaa tatttacctc gtgcatataa aaatggaaag aatgataaag
     2461 aagcaagaga aaaaatggca atggcatcta ctatggcagg aatgtctttt gcaaatgcct
     2521 tcttaggaat atgtcactct ttagcacata aattaggtgc attccaccat gttcaacacg
     2581 gtgttgcaaa tgcattatta ataaatgaag taatcaagtt caattgtgca gaagctccaa
     2641 ataagatggg tgcattctca caatacagat atcctgattg catacaaaga tatgctgaat
     2701 ttgcttcttt tgcaggtatt aaaggaagta cagaccaaga aaaagtagat aatcttataa
     2761 aagcaataga tgaactaaag gcaaaagtag gtcttcctaa gactataaaa gaggctggag
     2821 tagaagaatc taaattctta gaaagattgg atgctatggt tgaacaagct tttgatgacc
     2881 aatgtacagg tgctaatcct agatacccat tgatgagtga attaaaagag atatatctaa
     2941 aagtttatta tggtaaataa tatctattgt gcaacatagg ttctattggg tttgtatata
     3001 aattattaga tttgaatata aatagagttt tattagagac acgtagaagt aactataaat
     3061 aatagattat aatttaaatt ctaaatggaa atgataaaaa gtcagtaatt taatggtaat
     3121 ttcgaatatg tgtatttaat caattaactt taaaattagt cggaaaattt ttttattaca
     3181 tataatttta aagcataata tataaaaaac tttatgaaaa aattataaaa atat
//
LOCUS       DAELNY010000265         3219 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.268, whole genome shotgun sequence.
ACCESSION   DAELNY010000265 DAELNY010000000
VERSION     DAELNY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.268"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(185..862)
                     /locus_tag="KPE55_003532"
     CDS             complement(185..862)
                     /locus_tag="KPE55_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0494562.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSNTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(1051..2415)
                     /locus_tag="KPE55_003533"
     CDS             complement(1051..2415)
                     /locus_tag="KPE55_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0494563.1"
                     /translation="MKESDKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKECG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2543..3064
                     /locus_tag="KPE55_003534"
     CDS             2543..3064
                     /locus_tag="KPE55_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0494564.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1259 a          504 c          451 g         1005 t
ORIGIN      
        1 aaatagtcaa attaacttat tagaaacaaa aaatatacat aaaaaataaa tgtaaatatc
       61 aaataagata ctatataaaa atagatataa gtatcttttt tattattgaa attatgaaaa
      121 tatattttat tttacacaaa aaagagtgcc tcatgaacta aaaagttcat tttgagacac
      181 acttttacag tgtcaaagaa cgaagtttat ccttatccaa tacttttata ccaccacgag
      241 ataaagacac aataccttct ctagcaaaat atttcaacat tctcgataca acttctctgg
      301 cactacccat atacttagca atttgttcat gagtcatatt tatagtgtta ctgccatttt
      361 tagctatctc atcaattaaa aaagatgcca aacgcttatc aaaactcata aaaagaattt
      421 gttgcatagc ccacataaca tcagaaaaac gttcggtagc taatttatat gaaaataact
      481 ctgcatgaat attttctgta gataattttg caaaaacaga ggaactaatc tgtataatat
      541 cgcagtccgt ttcagcatct acatagacat caaaagttat tgtttttaga atacaggaag
      601 ctgataaaat acatacatct ccatcatcaa gtcggaacag tgtaacctct cgaccttcat
      661 ctgaaagtat gtatgtacgc actgttccag attttataat caaaatacca atacagtcac
      721 tatcaccaca atgtactgga ttcccttttt tgtaatgcga ggtatttgct gaagaaatta
      781 ataattcttt ttgtaagtca gtaagtttat cccaaaaagg caagctatgt gtaagtatat
      841 caatttcatt ttgtgaaatc atacaatatc tcctttctta aattgttata atcccatgat
      901 acaagtgaat agataaattg acaagtagtt tgttaagagg attatttagt cagatagtaa
      961 tagtatgatt tatgtagaaa aaatatgata atatatattc ttgctattaa tgagaaagta
     1021 atatgttttg atgatagtag gtttgtataa ctaatgcata ccgtttattt ttaaaagatt
     1081 aagttcctta tgtaacctta tagacataaa accagcaatt atagcagcca tcaaatcagc
     1141 tattggttgt gcatataaga taccatttaa cccatagaat acaggaataa tcaaaattat
     1201 agggataaaa cagacacctt gtctacacat acctaaaaat ccacattcct ttgcttttcc
     1261 tagtgcgagg aataatgtag aatacactgt atagaatcca aatagcataa atgaaaaccc
     1321 atttgcccta agagctttac taccattttc tattaataaa atgtcaccac ttgtaaattg
     1381 tgaaattata gattttggga agagtatcaa tataagacca agcactacac aaaatactgt
     1441 agtccataat atggaagttt ttattgctat atgtaatcta tcgtagttct ttgaaccaaa
     1501 attataacca gcaattggtt ggaaaccttt tataaaacca aatacaacaa gactacctaa
     1561 agatgttatt cgtgttacag cacccattcc agctatcaca gaatcaccat actttgtagc
     1621 ctccatatta gttaatgtaa tggcaagact agtcaataat tgaaatatta aggtaggtat
     1681 gccaattttt aggatttctg aaaaaatttc ttttgaaaaa cagcattcgt taattctaaa
     1741 actaaatata cttttcttgc ttaaaatata tcctacataa acaagtgttg aagcaatctg
     1801 tgagatagct gttgcaatag aagcacccct tacccccaga ccaaaagtat aaatgaatat
     1861 cgggtctaaa atcatattaa gcaaagcacc taataacata gcacacatag ttgttttagc
     1921 agctccttca cttgtgacaa tattagtcat agtgacattg aatatattaa aaatagatga
     1981 cacgacataa atgctagcat aagatattgc gtaaggcata atactttttg tagctcctaa
     2041 caattttaaa attggattga gaaaaatcat aatcacaata ataactaatg tacccactat
     2101 aatactactg taaatagatg tacttaccac ttttcctgct ttttcattat caccatggcc
     2161 taaaagtctg gcaatatatg aagcagctcc atttccaaac aataatccta atcccacaac
     2221 aagctgacct aatggaaatg caacggatat agctcccatt tgacttgttc caagtccacc
     2281 cacaaagtat gcatcaacca aattgtataa tgcagttatc atcataccta tcatggtagg
     2341 taatcccata gataacaatg cttttgagat tggggcagag cctaataatt caattctttt
     2401 gtcactttct ttcatactta acactcctct tctattgaca ttaagtctaa attataatac
     2461 tataaattat agtaagtatg gaaaatagta ctataattta tagtatttgt caatagtaag
     2521 aaaactaaag gagatgattt ttatggctac aatagattta attgtattgg gaatagtaaa
     2581 gcaacaaccc aagagtgctt atgatattca aaaggcagtg gaatacagga atatatcacg
     2641 ttgggttaaa ataagtacac catcaattta taaaaaagta attcaattag aacaaaaggg
     2701 atatattgaa agtaaaactg tgaagaatgg aaaaatgcca gaaaaagcag tttattcatt
     2761 aactgaatta ggtgagacac attttttaga tttaatgttt gatacatcta gcaaaccaat
     2821 aaatattctt ttagatttta atgctgttat agtaaattta aatagcctta caccagaaaa
     2881 acagaaatta tgtctcaata atattgaaga gaatgttcaa gagttaaaga aaactattga
     2941 ggaaaatata agtataaaga cagaagtacc agagacagca aaagcagtat tggaacaaca
     3001 gttggtgcta gcacaagcat tggaaacctg gataaattct ttaaaagata atatgggaaa
     3061 ttagaattag agaagctaac ttaataatca ttaaacaaaa accagcatat ttaataatga
     3121 gatattagaa aaaataaaac aaatgatata aactgaaaag tatatttaaa ataaagctca
     3181 cttaattgag ggagaatgtt aaacttttta agagcaaga
//
LOCUS       DAELNY010000266         3125 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.269, whole genome shotgun sequence.
ACCESSION   DAELNY010000266 DAELNY010000000
VERSION     DAELNY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.269"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..530
                     /locus_tag="KPE55_003535"
     CDS             15..530
                     /locus_tag="KPE55_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBG0494565.1"
                     /translation="MNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMTAQ
                     DLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISNQE
                     LSDIENKMFIKEALTKLPELEFEVIILRYYHDLKFKDIAKIMESSVSVTKYRMSRALK
                     KLELYLEGGKL"
     gene            527..1288
                     /locus_tag="KPE55_003536"
     CDS             527..1288
                     /locus_tag="KPE55_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494566.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFIILCTICIFLFSTSADSNSFYNTLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1326..2192
                     /locus_tag="KPE55_003537"
     CDS             1326..2192
                     /locus_tag="KPE55_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494567.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVKFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSVEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2213..>3125
                     /locus_tag="KPE55_003538"
     CDS             2213..>3125
                     /locus_tag="KPE55_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0494568.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNIVTNESGFSDF
                     KPDIYWKFFYPNKTFYNFIIESYSKPNDFNPEVIKSMPLENEINFYKFRNEKVNTILN
                     MEYPEGNYTQKEKDFWLSKNKDIKEPYLNGYYQGWEYFLLARDLLIFTIMAVCISIAS
                     VFSGEYQSGADSVVLTSKYGKTKVITAKIISSFLFGTIIFTINTIVSIGILLLSFGTD
                     GWNLPLQLTTSSIPYPYTFLQAILLSVL"
BASE COUNT         1270 a          338 c          503 g         1014 t
ORIGIN      
        1 cggaggtgat aatagtgaat gatgctcgga ttatatcaca aataaaagca ggaaaaaaag
       61 aattgtttga tactctaata aaaaaatact atgatagcat ctattgttat tgttatcgcc
      121 atgtagacaa taaaatgact gcacaagatt taactcaaga tgtatttgta acagttataa
      181 ataatataga aaattataaa cattatggta agtttgaaaa ctatttatat gttattgcaa
      241 aaaacaagtg taaggatttt tggaagaaaa aacaaccgat atatttagag tcattagata
      301 aggagatatc aaatcaagaa ttgtctgata tagaaaataa aatgtttata aaagaagctt
      361 tgactaaatt accagaatta gaatttgaag tgataatttt acggtattac catgatttaa
      421 aatttaagga tattgctaaa attatggagt caagcgtctc tgtcacaaag tacaggatga
      481 gtagggcatt aaaaaaacta gaattatatc tggaaggagg taaattatga ataataaaga
      541 aataaaaaga agtctacaga agtataaggt tcccaaatat gattcttcag ctcttgaaaa
      601 gactattaaa actatacacg agttaacgct tagtaatact tataaaagaa tgaataataa
      661 agactttttt atatctcaaa ttggttttat aagaaaaaga acttggatta tgcaatttat
      721 aatactatgt acaatatgta tatttttatt tagtacatca gcagatagta attcatttta
      781 taatacttta tccttgattt ctatagcatc accacttttt gtgctgacaa atatagaaga
      841 attaacaaaa gtatataata gtagtatttt agagatagaa ttttcaacaa aaaatacatt
      901 acataaaatt attgctacta gaatgcttat atttggaata agtgatttat tgtttatgat
      961 tattattttg gtatctgcaa aacaaatcat tgatgtaaac attctgtata taataattta
     1021 tatactagtt ccttttaacc taatgtgtat aggatgtatg gaaattataa atagaaataa
     1081 aggtaagcat acaaattatt attgtatgat atatggtata tctcttgtat tattaatagt
     1141 tacactgagt aactttagag ataatctata tcaatttgaa gcaataaatc aatggctaat
     1201 tgtttttcta gtcacagcag taacattaat atatgaaata agaaaaacgt ggagacatct
     1261 ttgttcattt gaaaatatta ttatctaagt aaaaaataag aatacacagt atcaggagga
     1321 atagtatgga gttaagtgta gaacgtttaa caaaacagta taacaataaa attatagtag
     1381 ataaaattga ctttaaatta aatgagggta tatatggatt tttaggagaa aatggagcgg
     1441 gaaaaacaac tcttatgaga atgatttgcg gaatattaag tcctacatct ggagaagtaa
     1501 aggtggatgg taaaaataat ttggatatgg gagaagattt tagagatatg attggatacc
     1561 ttcctcaaaa ctttggatac tacccagatt ttactgccaa agaatttatg ctatatatag
     1621 catcattaaa aggattaatg cctagctatg caaagagaaa aacacaagaa ttattagaaa
     1681 tggtaggatt atcagaggta attaataaaa aaataagaac attttctgga ggtatgaagc
     1741 aaaggctagg atttgctcaa acactactta atgacccaaa aattttaata ttagatgagc
     1801 caacagcagg gctagaccct aaagaacgag ttaaatttcg caacctaatt tcagaatttt
     1861 ctaaggaaaa gattgttata ttgtctactc atattgtgtc tgatgtagag tatattgcag
     1921 ataaaatttt aataatgaaa aaaggaagat ttgttttaac tggctcagta gaagaagtaa
     1981 cttcaaagat aaaagataag gtatggaaat gtgtggtcaa tgagaatgaa atagattata
     2041 tgtctgatag atattgttta gcaaatatcc atcattctgg tggagttgca aatcttagaa
     2101 ttgtaagtga tataaagcca catgacaatg catgcaattt aaaaccaaca ttagaagatt
     2161 tatatttata ttgtttcaac gaagtactat aaatatacat aaggagatag gtatgtctac
     2221 attaattaaa tttgagttaa aaaaaataat aaaaagaaag tctacaatag cagttatttt
     2281 aggaagttta ttcttaagca tcattctttt tgcattaatg ccaattcagg aaaaagctaa
     2341 tgatgaaagt ggaaaagagt ataggggttt acaggctatt gagctaaaaa agcaacaatc
     2401 taaattaaat gagggataca taacagataa aaaagtaaaa aactcaatca gagattacca
     2461 aaaaatgttt aataatccaa ataacattgt aacaaatgag tctggattct cagattttaa
     2521 gccagatatt tattggaaat tcttctatcc aaataaaact ttttataatt ttataataga
     2581 gtcatatagt aaaccaaatg attttaaccc agaagtaatt aaatcaatgc cattagaaaa
     2641 tgaaattaat ttttataaat ttagaaatga aaaagtaaat acaatattaa atatggaata
     2701 tccagaggga aattacacac agaaagaaaa ggacttttgg ttatctaaaa ataaggatat
     2761 aaaggagcct tatttaaatg gatattatca agggtgggaa tattttctat tggcaagaga
     2821 tttactgatt tttactataa tggctgtctg tataagtata gcttctgtat tttcaggaga
     2881 ataccaatct ggtgctgatt cagtagtatt gacttctaaa tatggaaaaa caaaagttat
     2941 aacagctaaa ataataagtt ccttcttatt tggtacgatt atttttacaa taaatacaat
     3001 tgtatctatt ggcatacttc ttttatcttt tggaactgat ggctggaatt taccactaca
     3061 acttacaact agttcaattc catacccata tacattttta caggcaatat tactaagtgt
     3121 gttaa
//
LOCUS       DAELNY010000267         3124 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.270, whole genome shotgun sequence.
ACCESSION   DAELNY010000267 DAELNY010000000
VERSION     DAELNY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.270"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..572)
                     /locus_tag="KPE55_003539"
     CDS             complement(<1..572)
                     /locus_tag="KPE55_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0494569.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTD"
     gene            complement(572..1432)
                     /locus_tag="KPE55_003540"
     CDS             complement(572..1432)
                     /locus_tag="KPE55_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494570.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1567..2367
                     /locus_tag="KPE55_003541"
     CDS             1567..2367
                     /locus_tag="KPE55_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG0494571.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2392..2961
                     /gene="yfcE"
                     /locus_tag="KPE55_003542"
     CDS             2392..2961
                     /gene="yfcE"
                     /locus_tag="KPE55_003542"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG0494572.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1168 a          461 c          380 g         1115 t
ORIGIN      
        1 atatcagtta cataatttga aagatatact cctccaactt tactataatt aaaatgcttt
       61 acttgatata caatcatact aatcatcttc aaaagacttc catatacaaa gtaaattaaa
      121 atacttacta taaaaccaca aactttcatt tttccatatc caaacttaaa aattaaaggc
      181 agtaaaatcg tcaaaattgc taaaaatata tttacagaaa aaaatacatc atttaaaacc
      241 atatttctaa agaacactag tgatgtcaat ttacttagta aggacacaat tattgttaca
      301 gaaattatta atcctacacc taataaatat tttgagtaaa ctatatcttc tatactaact
      361 ggcatactcc ttataaaaat ccttgaatca ttttcataat cacactcaaa cgtatttata
      421 aatatcaaat aactaacaac aatacttact gtataatatg aaattgaatt aaggaaaaaa
      481 taaaacacaa aaaatagcaa tatgtacttt aaattacgga taccatctga actaatccct
      541 attattaaat cttttcttat taaattgaac atcatctcac ctcatttaca taataataca
      601 ttagttcttc tatagtagga tttttgacct tatgcttatc aatttctatc aataggcttt
      661 ttactttcaa gctatctcca actttaacaa gtgcctcatt ataatattta tactctttca
      721 caccaactat gctttcttta attgcttcta actctgcttt atttgcccta attatcttat
      781 attcttgttc tattaattct ttcttaacag taaaaatctg atttcctcta tttataaaaa
      841 caagataatc agcaatttta attaaattat cagtattatg agtagaaatt attacactac
      901 tacttccatt tttcacaaaa tcttgtaaat actgcattat ttcagttctt acaattgggt
      961 ctataccagc agttggttca tccaaaatca ataacttagc attgtgacta agtgcgcttg
     1021 ataacataag cttttcagat tgacctttag ataaattttc aatatgcata ttttcttcta
     1081 tatcaaactt attaaggtat tcatcaaacc tttctctatc aaaattctta taaaataaag
     1141 aaactatttt tctaaattcc ttaaccttta aatgcggata aaattgtaaa gaatcatata
     1201 caaaaccaat attgttttta aagcttattg gagaattttc aatagtttca ttacaaagtt
     1261 caatttcacc ttcatctgca tcaataatcc ccattataat ttttattaaa gtcgtttttc
     1321 ccgaaccatt tggtcctata atcccaacaa tatccccttc ctttaactct aagtttatat
     1381 tatttaactt gaattttcct aatttttttg tgatattcct tatgctaaac acatcttcac
     1441 ctcttatgca tttttttata cccacctata catattttaa cacattcatt acaaaggtca
     1501 tttataaatt tataaattaa gaattataaa actatgttat aatacaaagt atgaagaggt
     1561 gaatatatga aaagaattat aactataaat acaaaccaat taaataagtc ccttgagact
     1621 aaagaactct taactagaaa acttatcaat gctggctttg aagtgtattc agatatttat
     1681 ccagacacag agcttattat atcaattggt ggtgatggct cttttttaag aacagttaga
     1741 gatttcgatt ttcctgagat tcccataatg ggaataaata caggtcatct ggggtttttt
     1801 cctgatattt taccagataa gattgatagc tttattgaag cttatacaaa aaaagattat
     1861 attattcaag aaatgtcact tcttaatgcg gaagtatata ctactactag tggttctaat
     1921 atgcttgctg taaatgaggt tgttataaga ggagacaagt ctaggactat acacctaaat
     1981 ttaagccttg ataataaaca tatacaaaat tttagtggag atggtatgat tatttctact
     2041 tccacaggtt caacagccta taactattct gctggaggta gtattgttga tataaatctt
     2101 gaacttatgc agattacacc tttacatccg ataaatacca atgcatatag gtgctttaca
     2161 tcaagtataa tatgttcaaa tgaatctgtt ataaagattg caccagaata tagatttgaa
     2221 gattctgtat tgattgtcgt tgatggagtt gagcacagat ttagacaaat cgaaaatata
     2281 aaagttagta tatctgatgc aaaaataaaa ttacttagaa tgtcaaacta tgagttttgg
     2341 catagagtat ctgaaaaatt tttatagact gtacttagga ggatttttaa tatgaaaata
     2401 ggaataatga gtgatactca tggtagctta ctttactttg aaaaagcgtt aaatgtatta
     2461 tctgattgtg atgttttatt acatggtgga gatgtcttat atcatggacc tagaaatgat
     2521 attccagaag gatataatcc taaaaaattt attgagacat taaataaatt agaaaacatt
     2581 gtgattgtaa aagggaactg tgatgcagat gttgaccaga tggttataga acatcctatt
     2641 cagagtcctt atgtcatgag ccaatttggt gaaattagaa ttatattgaa tcatggttat
     2701 atagaatcag aagaagaaat tattgacaaa gcaaagaaaa tgggtggtga catattagtt
     2761 ttaggacata ctcatgtaaa aaaactatac atggatgata atttaatagt aataaatcca
     2821 ggaagtactt ctatcccaaa agatggaagc cattctgtcg caattataga tatcataaaa
     2881 actgatgatg aggatgagct tgaacttgat ataaacttaa ttgacatcaa tactggaaat
     2941 ataataaata tcaatgatta gtaaattttt aataaaaata aaataagctt taatttatta
     3001 aactcataat aaaaacaagt aaaccatttt atttctatct caattagaag tttaagtaca
     3061 ataccttaaa cttctaatta aatagaatct ggtttacttg ttttattata tgctatattg
     3121 aaat
//
LOCUS       DAELNY010000268         2941 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.271, whole genome shotgun sequence.
ACCESSION   DAELNY010000268 DAELNY010000000
VERSION     DAELNY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.271"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            216..1139
                     /locus_tag="KPE55_003543"
     CDS             216..1139
                     /locus_tag="KPE55_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG0494573.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1114..1941
                     /gene="panB"
                     /locus_tag="KPE55_003544"
     CDS             1114..1941
                     /gene="panB"
                     /locus_tag="KPE55_003544"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG0494574.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1960..2808
                     /locus_tag="KPE55_003545"
     CDS             1960..2808
                     /locus_tag="KPE55_003545"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG0494575.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAI
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELIIAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1186 a          343 c          465 g          947 t
ORIGIN      
        1 tttttttact gatattttat tatataaatt ttatagaatt aattgtcaat tttttatgaa
       61 aatggtaaga tatttatgga tggtatcttg ttataagaac tatactttaa atgtttttaa
      121 ttattttgtc ttatgtaact catatcctca ggaatgagat tagaaattaa gatgttgtat
      181 cacaaagtgg aggtgatact attaaaatag gctttattgg ttctggaaaa gttggaacat
      241 ctttggcaaa ttatttttta tcaaaaggtt attgtgtttc aggcttttat ggaaggaatc
      301 aattatcttt attagaatca acaaatttaa caaaaacaaa gatttacagc aatttgaaag
      361 atataattta tgaaaacgat atattattta ttacaacatc agatgattct attgaaatta
      421 tagacaaaaa actttccaaa tttaatttaa aacataaata catttgccac acaagtggct
      481 ctttaaaatc tagtatattg ttctgttcaa aaaaagcagg tgcattaatt tattctatcc
      541 atcctatatt tgcattttcc aataaaaata ccaacataaa aaaaatgaaa gacatttgtt
      601 tttccataga aggtgataat gaacaagatg atttagttat tcaaaatttt atagatgatt
      661 tgggaaacca attttttata agagataagg atacttcatc cacttatcat ttagctaatg
      721 ttttagtttc aaatttagta ttatctttac tagatatagg agtaagttac tttattaaat
      781 taggtttatc tgaagaggaa tctttaaaag ctatcagtcc tttagtaaaa aaaaatatag
      841 aaaatatact tgataatggc tttactaaag ctcttactgg tcctgtttct cgtggagata
      901 taacaccaat aagaaaacat ctatctgtat tggataaaaa agatgaggat atttacaaaa
      961 tactatcctt aaacctacta aaaataattg ccttaggaaa tgacaattca ttaaaaggta
     1021 taaaaaatat tactatagaa aatgcaatcg aaaatttaat aagtaaatct gaaaaataca
     1081 aagaaattta caaactttta ggaggaaaaa acgatgaaaa ataccataca gacttttaag
     1141 aatgctaaat atgaaggaaa aaaactatct atgcttactg cttatgacta ctcaattgca
     1201 aaaataatgg atgagtgtga tataaatggt attttaatag gagattcatt aggaatggtc
     1261 ataaaaggtg aagaaaacac tctatctgtt accatagacg aaattatata tcatacaaaa
     1321 gctgtaaaaa atggtgtaaa aaatgcctta atcgtaagtg atatgccttt tctatcttac
     1381 catgtttcta tagaagatgc agttaaaaac gctggtagac ttataaaaga aggaggagct
     1441 catgctgtaa agttagaagg tggctcaaac gttataaagc aaatagaaag cattgtaaat
     1501 gctcaaatac ctgtaatggg gcatttagga ttaactcctc aatcagtaaa ttcatttggt
     1561 gggtttaagg ttcaaggaaa taccagtgaa acagcaagac aacttattga agatgctaaa
     1621 ttaatagaaa aagcaggtgc attctctatt gtccttgaag gagtgcctac gaaaatagct
     1681 gagatggtaa ctaattctat ttcaattcct acaataggta taggggcagg aattaactgt
     1741 gatggacaaa ttttagttta tcaagatatg ttgggaatgt ttggagattt tgttcctaag
     1801 tttgtaaagc agtatgcaaa tataggagat ataatgaaag attcaattaa aaattacatc
     1861 ctagaagtta atacaggtgc atttccacaa gaaaaacaca gcttttcaat aaatgaatct
     1921 gagttagaaa aattatacta atattaagga tggtacaaaa tgcttgtaaa agaaattaaa
     1981 ttactaagaa atataatcaa agattggaga aaacatggct attctatagg ccttgttaca
     2041 acaatgggat ttttacatga aggacaccaa agtcttataa aaaaagctat aaaagaaaat
     2101 gataaagtag tagtgagtgt atttgtaaat ccaactcaat ttggaccaaa tgaagatttt
     2161 aatagctatc caagagatat agataaagat tttaaatatt gtatggatag tggagcaaca
     2221 gtagtcttta acccatcacc agaagaaatg tatttaaaag gtaattgtac taccattaat
     2281 gtttctggac ttacagattt tctttgtgga gctaaaagac ctgttcattt tggaggtgtc
     2341 tgcctagttg tttcaaagtt tctaaatata gtaacacctg ataaagctta tttcggagag
     2401 aaggatgccc aacaattagc tgtaattaaa agaatggtca aggatttaaa catagataca
     2461 gaaataatag gctgtcctat aattcgtgaa aatgatggat tagcaaaaag ttcgagaaat
     2521 acatatctct ctgaggaaga acgaaaatct gctctaatat taaataagag cttaagttta
     2581 gcaaaagaag aattagtaaa aggcaatcta aatccagaga atataaaaga attaataata
     2641 gctaaaataa attctgaaca cttagcaaaa atagactatg ttgaaattgt agatagtgag
     2701 acattacaac ctgttaagca aatagaacat tctatacttg tggcaattgc tgtgtttata
     2761 ggaaaaacca gactaattga taattttaca tttgagttaa atatatagtc tataatttta
     2821 tataatatat ctaaaaaata atagaatacc taaaaagtaa taaattacct aaaaaataat
     2881 aaattgctta gaaaataata gattacaata actaaaattt taaaattaaa ttccttaaaa
     2941 g
//
LOCUS       DAELNY010000269         2905 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.272, whole genome shotgun sequence.
ACCESSION   DAELNY010000269 DAELNY010000000
VERSION     DAELNY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.272"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(112..1788)
                     /gene="bclA2"
                     /locus_tag="KPE55_003546"
     CDS             complement(112..1788)
                     /gene="bclA2"
                     /locus_tag="KPE55_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG0494576.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGAVGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLATNNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2166..2705)
                     /gene="hpt"
                     /locus_tag="KPE55_003547"
     CDS             complement(2166..2705)
                     /gene="hpt"
                     /locus_tag="KPE55_003547"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0494577.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
BASE COUNT          709 a          677 c          428 g         1091 t
ORIGIN      
        1 ttgtttatta ttttatagtc ttttcatcat ataaacctat taaatattat tctatttatt
       61 ggtatatgaa atcatattgt ttattatttt ataatctttt catcatataa actattgtat
      121 tctataaact gatacatatc cagctgcaga agtacctgtc gtgcctgaac caaatgtaaa
      181 acttaaagta acagctgctg ctctagcatc aactaaaaag cttaaactta cacttacatc
      241 tgatgaagta agtgtattta ctgcttttgc aataaagtta ttttgagcta ctccattaaa
      301 gttagtagta attgacataa atcctgcaag tgaaagtgtg gcacgtatac cgaaagatac
      361 attgtatatc cctccaactg caagatttat agtactactt gttggaaaag ttatattagt
      421 tccatttata aatgatgaat taaatgtcac taatgtatta ttcactaaac ttgaagagga
      481 cactgtaaat tgtgcattgt ttgttgctaa cactcctgtt gctcctgtcg ctccggttgg
      541 acctgttgct ccatttgctc ctgttgctcc ggtcgcccct gttgctcctg tcgctccatt
      601 tgctccagtt gggcctgttg gtcctatcgc tcctgctact ccatttgctc ccgttgctcc
      661 tactgctcct gttgggcctg ttgctccatt tgcccctgtt gctcctgtcg ctcctgttgc
      721 tcctgctact ccatttgctc cggttggacc ggttggacct attggacctg ctactccatt
      781 cgctccggtt gctcctactg ctcccgttgg gcctgttgct ccatttgccc ctgttgctcc
      841 tgtcgctcct gttggaccta ctgctccatc tgctcccgtt gggcctgttg ctcctactgc
      901 tcctgttgga cctattgccc cagctactcc tgttgctcct gtcgctcctg ttggacctac
      961 caaaccattt gctccggttg ctcctactgc tcctgttgga cctaccaaac catttgctcc
     1021 ggttgctcca gttgggcctg ttgggcctgt tggacctacc aaaccatctg gacctgttgc
     1081 tcctactgct cctgttggac ctacctctcc atctgctccg gttggacctg ttgctcctat
     1141 tgggcctgtt gccccagttg ttccggttgc tccagtcgct cctatacttc cggttggacc
     1201 tgttgctcct gtatttcctg ttggtcctgt cgctcctgtt actccatttg ctccggttgc
     1261 tcctgtattt cctgttggac ctgttattcc atttgctccg gttgctccgg ttgctccagt
     1321 tagtccggtt ggacctgttg ctccagttgc tcctgtattc cctgttggtc ctgttattcc
     1381 atttgctcca gtcgctcctg tatttcctgt tggtcctgta gagcctgtta ttccatttgc
     1441 tccagttgct cctttgtttc ctgttggtcc tgttattcca tttgctccgg ttgctcctgt
     1501 attccctgtc ggacctgtta ttccattcgc tccagtcgct cctgtatttc ctgttggtcc
     1561 tgtagaacct gttgttccat ttggtccagt cgctcccata tttcctgttg gacccgttat
     1621 tccattcgct ccggttgctc ccgtatttcc tgttggacct gttattccat ttgctccggt
     1681 cgctccagtt gcacctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc
     1741 tgttggacct acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct
     1801 ccttttttaa agttagagta ttactctata aatattgagt attactctat ataattatat
     1861 gaatcgacaa tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg
     1921 attcttgaat ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa
     1981 ttattaatac aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgtt
     2041 ataaaaaata gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta
     2101 aataaaaaaa cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata
     2161 attgcttatc tatcttcatc agtaacattg aatatataag gaacatttct ataataatca
     2221 ccaaaatcaa acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct
     2281 ggcactaaat cgacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta
     2341 gcaggatttt tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta
     2401 atatcgtcta caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaactact
     2461 ttaacccttc cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact
     2521 ctaagtggta aatttatatt tctaactaag tctgcacaaa atataaagct accttttaat
     2581 agagaaacta ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc
     2641 cttaatttat ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata
     2701 tccataaaaa gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt
     2761 tttcaatact cttattcaat attagtcaat attattgagt ttttatgttc attcaacaat
     2821 tattaacaat gtatcttata ttttaagtag ttcatatatc cacactaaat tacatactat
     2881 atattttaga caaaaaaaga aaaaa
//
LOCUS       DAELNY010000270         2903 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.273, whole genome shotgun sequence.
ACCESSION   DAELNY010000270 DAELNY010000000
VERSION     DAELNY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.273"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            19..1128
                     /locus_tag="KPE55_003548"
     CDS             19..1128
                     /locus_tag="KPE55_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0494578.1"
                     /translation="MDFKSLGISENTINILKKLGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDHLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRSLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1505..2689)
                     /locus_tag="KPE55_003549"
     CDS             complement(1505..2689)
                     /locus_tag="KPE55_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494579.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1096 a          443 c          403 g          961 t
ORIGIN      
        1 atgaaaggta agataacaat ggatttcaaa agcttaggga ttagtgaaaa tacaataaat
       61 attctaaaaa aattaggaat aacaacacct acacctatac aaaaagaaag tattaaacta
      121 ataaaagaag gaaaagatgt aatagcagaa gctcaaactg gaacaggaaa aacactagct
      181 tttttacttc cgatatttga aaatatatca ttagatataa atgatataca agtacttata
      241 ctttctccta caagagaact agctattcaa ataacagaag aagctatgaa acttaaagaa
      301 tctaaagatg taagtattct tgcagcatat ggaggaaaag atattggttc tcaaataaag
      361 aaactaaaag gaaatattca tatgattata gctacaccag gtaggctttt agaccatcta
      421 aatagaaaaa caatagacct tagtaagttg aagacttttg ttttagatga agcagaccaa
      481 atgttgctta tgggatttaa aaatgaagtg gaagctattt taaaagagac ttcaaacaaa
      541 aagcaaacac tatgcttttc tgcaactata aattctcaag taaaaaaatt agcttataga
      601 tacacaaaaa atcctgttgt agtttctatt caaaaagaag aaatcactct taataatata
      661 aaacaagaag tagtagaaac tacagataga aaaaaattag atgccttatg taaggtacta
      721 gatgaagata atccttttat ggcaataata ttttgtagaa ctaagagaag ggttgaccat
      781 cttgaagaag ctcttgctat aagaggatat aattgtcaaa aacttcatag tgatattgct
      841 caatcaaaac gagaaagaat aatgaaatca tttagaagtt tagatataca atacttaata
      901 gctacagatg tagcttctcg tggtcttgat atatctggag taagtcatat atataattac
      961 gatttaccag aaacaccaga agactacatc caccgtattg gcagaactgg tagagctggt
     1021 gaagaaggat atacatgtgc ttttattgac cctaaaaatg aacgaatgtt aagtgaaata
     1081 gagacggcta tagaatctaa aattagtaga agaattatag aattataatt tatagattgc
     1141 ttaaaatacc aatgccacac aaacactgta atatatagaa agctacagaa aacatgtatc
     1201 aatctatact ttgctgttat actattttat attaaagaaa aaagaggcta tccaaagtag
     1261 gattagtctc ttttattgtc aaaataatga aaatatactt gacttcatat aaaaaagagt
     1321 gccttataaa ctaaaagtcc attttgagac accctctttt tatcttttat tcttagtttt
     1381 atattgcttg tttatagttg tttaatatta gattttgcaa ataatcttaa taaacatgca
     1441 gatatattat ctatagtatt cttatctata gtattctctt tagcaatatt ttttgctact
     1501 acatctaatt tacattttca tttaattccg ctggccattt agcttctgga ttttcctttg
     1561 caaacttctt attcttataa tgtcctagtg taaggaatgg aattactata gtgaatagac
     1621 caagataacc acaatatcca tatccatatt taacaatatt acttaatcct gtaagtgata
     1681 taagcattga cactaccatt attgcacaag ctgtaaatac ccttcttttc attcttgaag
     1741 aaaggaaaga aagtgcttta tggtcttcaa atctatttac aagaccaaat atagtagtta
     1801 ctcctgtaga cattaaacaa aggaataatg caacattata aattatgtat atagtatgat
     1861 ttccagattg acctgcaaca taaagagtag ggagtgttgt ttgcccagca gcaataattt
     1921 ctctatacca tcccattagc atagttacag caagtcctaa tgcaattgca ttcataacaa
     1981 atgaaattat cattgcctta gtagcttctt ttgagttctt aagaagtggt cttgaacatc
     2041 ctgcaagtgt aggtataaca accgattgaa aacctgcata aataaaagta ttaagtattg
     2101 gcttgatata gccaggtgtg aaactcgttc tatttgataa tatttcagtt atttctggta
     2161 ctttagcttt tataccaaca ataaaaatag taagagtaca tactagaata gctattgtca
     2221 ttgctgtagc tgctttagat acaagcatag caccaaatat agtaaagaca agaagtacaa
     2281 ctccaataat aaatacagct acaaaataat ttactcctat actttgaaat actgctgctg
     2341 cacctgctat aacagcactt acagctatta aaaccattag atagtaatat atctcccata
     2401 ttatttcaag ttttggatat ggtctccaaa gctcacaaaa taaatcctta taattcctac
     2461 aattattatt attatacatt atgataactt ctctcatggt agcagtaagt ataatcattg
     2521 ctaaaattgc cataagtggt gctgtccatc catactgcac atagtactga gtttcttgat
     2581 tccctgtagc aaatcctcct cctgcatgtg aactaaaaag aacgcttgca acaccaaaaa
     2641 tggctaacat agagcccgtt acttttgctt tctttacact tttttgcatg aaatcatcct
     2701 ccctaaaatg atattaaaag tttttatttt aacactttat tgataaaaca ataacatttt
     2761 tgctcattaa attcaactat atttttccaa tgactttaca ttttcaactt atttctacct
     2821 ttatattaaa tattttttat atatttattt tattttttaa atagtattca aattcacttt
     2881 gttttttaat tttatgaaat atc
//
LOCUS       DAELNY010000271         2810 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.274, whole genome shotgun sequence.
ACCESSION   DAELNY010000271 DAELNY010000000
VERSION     DAELNY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.274"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            567..707
                     /locus_tag="KPE55_003550"
     CDS             567..707
                     /locus_tag="KPE55_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494580.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(704..1075)
                     /locus_tag="KPE55_003551"
     CDS             complement(704..1075)
                     /locus_tag="KPE55_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494581.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1624..1881
                     /locus_tag="KPE55_003552"
     CDS             1624..1881
                     /locus_tag="KPE55_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494582.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1991..2671)
                     /locus_tag="KPE55_003553"
     CDS             complement(1991..2671)
                     /locus_tag="KPE55_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0494583.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMDTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT         1172 a          297 c          317 g         1024 t
ORIGIN      
        1 tgtatagtta aatgtgaaaa tgagtatgaa agaatgcagt gtctatgaaa agtatatttt
       61 aatatgaaac taaaactgga aaaataagaa tagcagaaaa aatgattata ttataaatca
      121 gtttttttga gacatctggc ttagattctg aaagatattt aatcaaagaa ataaaactat
      181 taaataagca agtaaacagc taaaatagta tgatttttat agaaaattta aatccatcct
      241 aaagaattaa aagaaagtca aataaatata aaaaaattat atatttataa aaaattttat
      301 aataaaaatc atcctgaaaa atatataaaa atagaaaaaa catacaagat tctacaataa
      361 tagatttttt tgttattatt acgagttttt tattgcaact gtagaataaa agtattatta
      421 tataattaag ataattatct agggaaattt tggataatca aaatatattt tataaaatca
      481 aaatacaatt acataaactt tattaaaaat ttgttgggaa tttctgacta acattaagaa
      541 cgtaagtttg ttgggggatg gtatatgtgg aaaagtcaaa cactactgtt acaaaaaaag
      601 aaaaaattga agatatagga atgaaattag aacatttatt gagtgaagat attacttttt
      661 atgaattatt acttgagatt ttgtataaag aaaaagaaga gaattaattc tcttctttaa
      721 ggaatctttt taaataggca ttatcaatca ttttttctat agcttctcta tcttcttttt
      781 caagagaata tattttttct aagagctttt ttgttttttc acttactgat aagttctcta
      841 tgattaattt atcttctttg cttatttttg tattatcatt tacgcctaat aaataatctg
      901 ttgatgtttc taatgctttt gctaatctaa ctattatttc acttttaggt tctcttaaat
      961 tattttcata acgtgacaag ctagcctcag ttataccgac tttagtagca agttcttttt
     1021 gatttatatc taattctttt cgtagatttg ctattctatc tcctaaactt tccatatgta
     1081 catcactcct taccgaaatt ataagttatt ttgataaaaa gtaaaataaa attaccaaaa
     1141 atataaaaaa aaaaggttaa aataataaaa tatattgaaa attaccattt gtatatgtat
     1201 aatatgaatt atacaaatgg taatttgatg agaggtggta tgttgaaata agaaactgaa
     1261 ttaattaata aatacataga aagttttgaa aacctttaat tgagatataa acaatatgta
     1321 caaaattttt tgcaagtaaa attaccaaat gtataaaata aatcagtagc atagataatt
     1381 ttatgcttta atacattaga ttatttaatt tagttataat ggtcaaaaat gcaatctaca
     1441 aataaaacaa agtattatcc aaaaagtatt ctttaagaaa attttttatt agagatgtaa
     1501 ttgatataaa taatcgtatt aaaaaataaa atactaagtt taaaaattta aataatctat
     1561 aaatcaatta taaaatatta tttcttagaa attaaaatga attaagtaaa tggaggaatt
     1621 gatatgataa taatcagaag tcaagataaa actaatttaa tgcagataaa ccaaattaaa
     1681 atagatggta gtcaagttta tgcagtattt gaaagtaaaa ttgatactgt aaaaatagga
     1741 gattatgaaa ataatacaag agctatacaa gtgttggaca atatacaaaa cttcattgaa
     1801 aatggaacaa aatatgatta tataacatca aataaagtca gatacaatgt taataaaata
     1861 tttcagatgc cagctaaata gtgagtgtgc attttaattt aataactaaa gcccactttc
     1921 ttttgtagta tgaaagaggg ctttacatat ttgtaaatat cgaagtacac ttgttttatc
     1981 aataaattat ttaataaggt ataatggacc aattaaacaa taaaaattct cctaaaacag
     2041 caagtattaa atatataata gcttcatttt tgtaaagaga ggtaattttt tcgcttatgt
     2101 catcatcaat tgtaatagca atttcatctt gaataatatt tttatatata gatgggaaaa
     2161 tatctttttt ctttataaga ttaaataaat tatttacacc tacccatatc aaatatccta
     2221 aaacagcacc taatgtgtcc atcattaaat catcaatatc tgtagctctt aagttaaaca
     2281 attgacaaag ttcaattgct aatgaaaaac ctaatccagt taatgaaaca ttgataatat
     2341 ttctaaaatt tttccaaatt aaaggtagta aaaatcctaa aggcataaac ataattatat
     2401 ttaaaatata tgttaagatg ccaactgagt caaaaggtat tagatttatt tcatcaattc
     2461 taaaaagccc actatacctt cctatatccc atatgctacc aattccagct ttactaagtg
     2521 ctagatagat atataataaa aaaatatata cccatactaa atgtatcatt acattctttt
     2581 caactttcaa attttttcgt tttataacga ctatttggta tattaagcat ggtaatataa
     2641 cgcaaataaa tgaataaaat gtccatatca ttttattttg tcccctttca aatttatgtt
     2701 tttagtttta taagtactat ctttatatta aactgtaagt tttaaaaatc ttttaataaa
     2761 atcttaaaaa tttattaatt tataaaaata tattattaag attaaaaaat
//
LOCUS       DAELNY010000272         2800 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.275, whole genome shotgun sequence.
ACCESSION   DAELNY010000272 DAELNY010000000
VERSION     DAELNY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.275"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            247..2031
                     /locus_tag="KPE55_003554"
                     /pseudo
     CDS             247..2031
                     /locus_tag="KPE55_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1237 a          397 c          501 g          665 t
ORIGIN      
        1 acaattcatt aacataaaaa tcaaacttac cttaaaatat aaatttacaa atttttcaat
       61 caataagaaa atttctttat tccttacata atgactgcaa tgtaacaaaa taattacaaa
      121 ttaattatga aatcattata gttagaaacc tctttaaaat tagatattaa gtatccttta
      181 gaattagaac gggaacaaag ttaaaaaatt aagtaatatc aaatggacaa gggggaagaa
      241 aataaaatga aaataccaaa gaagattgct gcaatgctta cagttacaat gatagctgga
      301 agttcaacag caggcatagc tagtgcacaa actgtggcaa caaatttaac agggcaagaa
      361 agatatgaaa ctgctgttaa aataagtcaa gatggatgga aaaatgctga tgaggtagta
      421 atcgtaaacg actcatctat agcagatgct ctatctgcaa caccatttgc aaaagcaaaa
      481 aatgctccaa tacttttaac aagtaaagat aaattaaatg ataagacgaa agcagaaata
      541 caaagattaa aagctaaaaa agtatactta ataggtggaa cttctgtatt aagtacaaat
      601 atagaaaaag aaataaaaaa tttaaaaata tcttttgaga gaatctctgg agctgagaga
      661 tatcaaacat ccttagaatt agcaaagaaa ttagatgcta taagtgatgt taagaaaata
      721 gctgtagtta atggtgaaaa aggacttgca gatgctgtaa gtgtaggagc accagctgca
      781 caaaacaata tgccagtaat acttgctgat tctaaaaatg gaacagcagt tgcagacaaa
      841 tttataaaag atgctggaat aactcaatct tatgtagtag gtggagagag ttcaatttct
      901 gaagctgtta aaaataaact accaaatagt acaagactag gtggaacaga tagaaatgat
      961 acaaatgcaa aagtaataaa agaattttat aaaaagacag atttaaaaaa tgcttatgta
     1021 actaaagatg gtatgaataa acaagaccaa cttatagatg cactagcagt aggtgtatta
     1081 ggtgctaaaa atcaatcacc agtagtttta gttggaaaga acttatcagc ttcacaaaaa
     1141 tctctagtta actctaagag ttttgataag ataactaaag ttggtgggaa tggtaatgag
     1201 acagcattta atgaaatgaa gtcattacaa gaagtaaaaa cagttgaagc taaaacaatt
     1261 tctgaattaa aatcagctat agataaagct actgctaatg atgttataaa ctttaagcca
     1321 actagtgagg ttaaagaagc atttacaata caaactgata aagcagtaac agtaaactta
     1381 aatggtacat atacaaagac tgtaactatc aacatgccta atggagatgt aaataactat
     1441 gcaaaagtgg atgatttagt tatagatgat gtaaaagatg gaacttttgt taactatgga
     1501 aaaataacta acttaaaggt taatgataaa aatggagcca agatagaaaa taattctaaa
     1561 ggtgagattg gtagtttaac agtagcatct ggagcaagtc aagtaaaagt gactaatggt
     1621 ggtaagataa ctacagttac aaataattct aaagggacta ctatagataa taaaggtaca
     1681 ataagtagtg ttaagggaga taattctcca acaataagtg gaaatagtcc aagttctaat
     1741 tcatcaggag gaagttcatc aagtggaggt tcttcacatg gaggaggctc atcttcagga
     1801 ggaagttctt ctaatcaaac atcagtaaat aatgaagcag caaaaataac aagcgtgcct
     1861 acaccagcaa aagatgcaac tagattaact atgccatcag tatcaagtgg atatactata
     1921 gcaataaaaa catcaagtaa tgagagtgtt ataaaaaaag atggaacaat aatacctcca
     1981 aatacagcaa caacagtaaa attagtattt acggtaacac atacatcaag tggtaaaaca
     2041 gcagatacaa aagaactatc tgtaacagta ccagctaaat caacagatga agaattacaa
     2101 gcagcattag acaatgaagt ggcaaaaata acaagcgttc ctgcaccagc aaaagatgca
     2161 actaaattaa ctatgccatc agtatcaagt ggatatacta tagcaataaa aacatcaagc
     2221 aataagagtg ttataaagga agatggaaca ataatacctc caaatacaga ggaaacagta
     2281 acattggtat ttacagtaac acaagaatca agtggtaaaa cagcagatac aggagaaata
     2341 gatgtagtag taccagccaa atcaacagat ggggaaatac aagcagaagt acaagctgag
     2401 gcggataaaa taacaagtgt aactcagcca acacaagatg caacaacact aactatgcca
     2461 acagtagcaa gtggatacac aataaagata aaatcatcaa caaatgaaag tgttatagcg
     2521 aaagatggaa caatagtgcc accagataca gaaacaaaag taacattagt atttacagtg
     2581 acacacacat caagtggtaa aacagcagat acaggagaaa tagaagtaac agtaccagcc
     2641 agaacagcta tagatacaga acaattggca gtacaagctg aggcagataa aataacaagt
     2701 gtaactcagc caacacaaga tgcaacaaca ctaactatgc caacagtagc aagtggatac
     2761 acaataaaga taaaatcatc aacaaatgaa agtgttatag
//
LOCUS       DAELNY010000273         2787 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.276, whole genome shotgun sequence.
ACCESSION   DAELNY010000273 DAELNY010000000
VERSION     DAELNY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.276"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1280
                     /locus_tag="KPE55_003555"
     CDS             <1..1280
                     /locus_tag="KPE55_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG0494584.1"
                     /translation="SSKYMLSLNGKKSYGFVNIMHFTLKIVFISFLILSINNCILNYN
                     MLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEKDAFLI
                     DSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIFNLIDY
                     DDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKSDLNVN
                     IIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFNSKKLD
                     ALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISNLMITY
                     NIVASYYERNKYKLYIKKIFGYSATARSMLLIVSLILTNIIPIGIISTRVDLSNNIIL
                     FGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            1285..1902
                     /locus_tag="KPE55_003556"
     CDS             1285..1902
                     /locus_tag="KPE55_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494585.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            2091..2435
                     /locus_tag="KPE55_003557"
     CDS             2091..2435
                     /locus_tag="KPE55_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG0494586.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT         1186 a          268 c          397 g          936 t
ORIGIN      
        1 gaagttctaa atatatgcta tctctaaatg gaaaaaaatc ttatggtttt gtaaatataa
       61 tgcatttcac attgaaaatt gtatttatat catttcttat attatccata aataactgta
      121 ttttaaatta taatatgcta caaactcagt taggaaatct atctgagtgg gataaagcaa
      181 aaaatgttta taatcttact cttaaagata caggtgaaca atctttagga aaggaagaag
      241 taattaaaaa tgccaagatt aaaggtttct ataaaaattt ggtagaagaa aaagatgctt
      301 tcttaataga ttcaactaat tttgaaaaac ttgaagatgg aagttacatg tatgaaataa
      361 attcaaaggg taaaaaccca gaagtaagtc aatatggtaa aaatataaaa ataaataaaa
      421 actatcttaa agttaaccct atttactcaa atggcaaaga aatatttaat ctaatcgatt
      481 atgacgataa gactataaat ttattagttc ctaaaaagtt acaagtacat gaaaatgaga
      541 ttaaaaaaga gtttagagaa ttattttatt ttgaaaaaat agaagtagaa aatatgtaca
      601 ataaagagtt aaatagagag ttaaataaaa caaagaagtc agatttaaat gttaatataa
      661 tatatgtaga taataatcaa agctatttta cttataactc ccttgttatg gatgacaata
      721 ggaatctcat tgatgaccca atagcaatag ttgacataga taatattgat gattcttttt
      781 atctttctta tatatctaga tgtgtatatt ttaattctaa gaaattagat gcccttgcag
      841 atataagtaa cattattgaa gctcaaggtg tagaagcgca tatccaaagt ttacatgctg
      901 tttataatga gtatggttta gaaataaata aactagaaaa atatttgaat agtgaaattt
      961 ttactataat aataatagca atatcaaact taatgattac ctacaatata gtagctagct
     1021 actatgaaag aaataaatat aagttatata ttaaaaaaat atttggttat tcagctacag
     1081 ctagaagtat gttgttaata gtctcattaa tattaacaaa cattattcca ataggcatta
     1141 tatcaacaag agtagattta agtaataata ttatcttatt tggacttcta atattagtta
     1201 tagaagtaat tgtatctatt gtattagaca agattatctc aaatagttca tttaataaaa
     1261 ttataaaagg agaacattaa aaagatgatt tcattaataa atatatctaa aaaatttaaa
     1321 gataaatcta taatagacaa atttaaccta gaaatttcta agggcgagtt tgtagcgata
     1381 acaggaaata gtggttcagg taagacaact ttacttaata taatgggatt attagaaaaa
     1441 cctaattctg gtgatattat aataaataat ataaaaaatc caaatagtaa acagataaga
     1501 attttacaaa gggattttta tggctatcta tttcaaaatt atgctttaat tgagaatgaa
     1561 aatgttgaaa ataacttgaa aattgcttta aagtatcaaa aaaatatcaa ccataatgaa
     1621 aaaataaatt acgcacttga gtctgtaggt cttagaaaat acaataaaaa aaagatatac
     1681 gaattgagtg gaggagaaca acaaagggta gcattagcaa gagtaatatt aaaagaatgt
     1741 gaagtaatat ttgcagatga acctacaggt aatttagata aaaaaaatag agacatagta
     1801 tttgatatac ttaaaagtct aaataagaat ggtaaaacta tcgtatttgt aactcatgat
     1861 ttagagttat cggagcaggc agatagagtt ataaaattgt aataatatca actataataa
     1921 atataagaaa gagattacag ctaaatcaag tttaataata atacgaatag aatttaaaca
     1981 ttaagtaaat tagtagtttg taaatataag tataatatga tatcataaag gctcaaaaga
     2041 aattttatat aataagaaat ctaaaaataa caaatggagg acatataaag atggaaaatt
     2101 taccaaattg cccgaaatgt aattcagaat atacttatga agatggaact cttttagttt
     2161 gtccagaatg ctctcatgag tgggcattag gattgggagc tgaagaagat tcaaatatta
     2221 tcaaagatgt aaatggaaat gtattaaatg atggagattc tgttacagta attagagacc
     2281 ttaaagtaaa aggaagttca tcatctataa agattggaac aaaagtgaaa aatatccgtt
     2341 taatccatga ctcatcagat ggacatgata tagaatgtaa gatagatggt tttggagcta
     2401 tgaagctaaa gtcttctgtt gttaaaaaag cgtaatatta taaataagct gtagtgttta
     2461 ttgtttttca gtacagtata aaaccataag aatctattaa gtaataaaag tttgtttatt
     2521 tatctttata ttttcttatg gttttattaa atataaattt tattgaacac caaaatattc
     2581 tctcctctac aaataagcca atcttattta aactaatata ctttgtacta tttatactga
     2641 aattgtaaat ttaaatgcta tgttatttag gtttgtatag ttaatttata tatttctgat
     2701 tttataaatt gtatttatta ttatatcttg attctaaaat cattaaaaat ttcaacttct
     2761 ataaagattt acaatttatc aaataca
//
LOCUS       DAELNY010000274         2760 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.277, whole genome shotgun sequence.
ACCESSION   DAELNY010000274 DAELNY010000000
VERSION     DAELNY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.277"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            274..2409
                     /gene="rnr"
                     /locus_tag="KPE55_003558"
     CDS             274..2409
                     /gene="rnr"
                     /locus_tag="KPE55_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG0494587.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1209 a          260 c          507 g          784 t
ORIGIN      
        1 attaggtgat aaagagtaat ttaagcaatt atgataaaga ataacttaag taattataat
       61 gatatttaca aataatttat aaaacataat aatttgtttg aagttagtat gacaaagctt
      121 acattaaatc aaaaatataa ataagtaatt ttaacttgta ataataataa ggaaatatag
      181 aaaattatgt gataagtaaa aaatgttttg tctgaagaaa tataaaagac aatataaata
      241 ataatataat gaaaagtaaa acaggaggga aaaatgatac caggacttaa agaaagtttg
      301 ctaggattaa taaatgaaag tgcatataat cctctaaaaa aagaagaatt agcagaaata
      361 tttgatatac attcatcaga aatgccaatg ttttataatt ttttaaatga attagaagaa
      421 gatggttata tctgctttac taaaaaagga aaaatagtat ctcccaatca aatgggatac
      481 tttgttggaa aatttgtatc acacaaaaag ggttttggat ttgtagaatc tgatgttgaa
      541 tatacacagg atttatttat ctcaagtgac aatataaatg gtgctatgca taatgataga
      601 gttatggcag aaatagtagt acctgcaact gaagaaaaga gagcagaagg aagaatagta
      661 aaagtaataa agagagaaat aactagaata gtaggtacct tccaatcaag taaaactttt
      721 ggatttgtaa caccagacaa caagaaattt actaaagata tatatatacc taaaaaacat
      781 tttagtggag cagtagataa tgataaggta gtatgtgaaa ttacagtatg gccacaagag
      841 gatagaaaac cggaaggcaa aattatagag atactaggtc aaaaaggtga aagaggcgta
      901 gaaatagatt ctataataag agaacatggt cttccagaag aatttccaaa gaaagtattg
      961 caagaagcag aagctgtagc tgttgagatt cctcaagaag agataaaaag aagaagagat
     1021 ttaagagatt taaacatatt tactatagat ggagatgacg caaaagactt agatgatgcc
     1081 atatcaatag aagtattacc taatggaaac ttcaaactag gagttcacat agctgatgtt
     1141 acacattatg taagagagaa aaataaactt gacaaggaag ccttaaaaag agctacatca
     1201 gtttatttag tagataaagt aattccaatg ttgccaaaaa ctctttcaaa tggagtatgt
     1261 agcttaaatc catttgaaga taagttaact ctttctatat ttatggaaat agaccataaa
     1321 ggaaatgtgg ttaaacatga aatatgtgaa tctgtaataa attcaaaagc tagaatgact
     1381 tatacagaag tatctgatat attagaaaaa gatgatgaaa aacttaaaaa gacttttgaa
     1441 catgttgttg aagattttaa aaatgctgaa attttagcta ggattcttat gtcaagaagg
     1501 gaaaaaagag gagctataga cttcaatttt ccagaggcaa agataattct aaatggaaaa
     1561 ggcgaagttg tagatataaa gccatatgaa agaagaatat ctaataagat aatagaagaa
     1621 tttatgctta taagtaatga gacaattgcc gaacattttt actggatggg aataccgttt
     1681 gtgtatagaa tacatgagac accatctttg gaaaaaatgg aagaattgag taagtttata
     1741 tctacatttg gatatataat aaaaggtgat aaagaggaag tacatccaaa agcattacaa
     1801 ggtataatag agaaaataaa gggcaaaaaa gaagaaggag ctatcagtac tataatgctt
     1861 cgttctttaa aacaagctaa gtactcacca gaatgtagtg gacattttgg attagcagct
     1921 aaattctact gtcactttac ttcaccaata agaagatatc ctgaccttca aattcataga
     1981 ataataaaag aatctttaaa taataaaata agtggaaaaa gacaagaaca gcttactact
     2041 atagtggatt atgcatctgt tcaatcatcc gagaaagaaa gaaaggcaga gttagctgaa
     2101 agagatgtac atgatttcta taaggctctt tatatgaagg ataaagttgg tcaagaattt
     2161 gagggtgttg tatctagcgt aacttcattt ggaatatttg ttgagttaga taatactata
     2221 gaaggattga taagacttgc aaatatgaat gatgattact atatttatga tgagagttct
     2281 tatagtgttt taggagaaag aactaagaag tcatataaga taggagacct tgttaagata
     2341 aaggttgaaa gtgttaatgt agattttaaa gaaattgact ttgagatagt tgaaaaaata
     2401 gaggagtaga taaaacagct aaaataagag ataacaagtt gtagtattta tgatatgtat
     2461 aaatatatag tgcacaatac tctataaaat aaatgataaa aataggtaat tgagaaatat
     2521 ctaatggaaa tagttaactg aaagataaat aattaagaaa tatttaatga aagtagctaa
     2581 ttgaaaaata agtaattgag aaatgtttaa tagaaagagc taactgaaag ataagtaatt
     2641 aagaaacatt taatagaaaa agccaactga aaaataaata attaagaaat gtttaataga
     2701 agtggctaat tgaaaaataa gtaattaaga aatgcttaat agaagtggct aattgaaaaa
//
LOCUS       DAELNY010000275         2671 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.278, whole genome shotgun sequence.
ACCESSION   DAELNY010000275 DAELNY010000000
VERSION     DAELNY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.278"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(180..731)
                     /locus_tag="KPE55_003559"
     CDS             complement(180..731)
                     /locus_tag="KPE55_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494588.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            922..1884
                     /locus_tag="KPE55_003560"
     CDS             922..1884
                     /locus_tag="KPE55_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG0494589.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2178..>2671
                     /locus_tag="KPE55_003561"
     CDS             2178..>2671
                     /locus_tag="KPE55_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBG0494590.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIP"
BASE COUNT         1005 a          289 c          422 g          955 t
ORIGIN      
        1 tttgtactcc atttgtcaaa aatataaaac ttttatataa gcttattata acataattga
       61 taaaagtgta aagatatata atgaatttat aaagaaaaat aaaataaaaa agataaatcc
      121 taatcaaagg atttatcttt ttgtatagaa aataattatt ctattattaa atggcatggc
      181 taaactggat aataatttat tattactgct ttcaatgttt tttctccttg attatggtat
      241 gtatgaattg attcagctga aaaacaaata gagtcatttt ctctaagttg ataaatagag
      301 tcatttactt ctaattttaa ttgaccttct ataatcataa tatattcctg tgatttttca
      361 gaatgaccaa cagatgtata actatgacct tcttctaatt ccatttgaaa taattcgaag
      421 tttctagttg gtgtattggg ataataacaa taaagacgat aatctttatt ttctgaaact
      481 tgtacatcta tatcagtttt tgatacaata aaagtttcat tttgaggttg ttccagtatt
      541 gcagtatatg gaacatttag tccacttgca attttccaaa ttgtgtttac tgttgggttt
      601 gagtctccct tttcaatttg cgaaagcatt actttactta cgccagataa ttcagcaagt
      661 tgacctagac ttaagttacg ttcattacgt aatctattta aatttttagc aataacagta
      721 ttaatactca tagtagctcc ttaaaatgta aattattgac aaatataatt tacgaatgta
      781 taatttattt aacattattt ataatttaca aatgtataat ataatgaacg ttaaatacac
      841 tgtacatccg taatataaat tatactgcat aaagagttgt ttttcaacgg atgatagttg
      901 agagattaag ggggaattgt aatgcgtgta cttaataatg ttacagaggc tataggcaat
      961 actcatatgt tacatttatc caaaatagca gatgaatttg gagttcaagg taatatttat
     1021 gcaaagatgg aatacttaaa tccgggattt agtaaaaagg accgtgtagc attacaaatg
     1081 attaccgaag cagaaaatgc aggtttatta gttccaggtc aagcagttgt ggaattaaca
     1141 agtggtaata ctggaacagg actagcaata gcttgtgcat gtaaaggata taaatttata
     1201 gcgtgtatgt caaaaggaaa ttctatggaa agagcaagaa tgatgaaagc tttaggtgct
     1261 gaagtagttc ttgtagacca aatgcctaat tcagttcatg gacaggtatc tggtgaagat
     1321 ttggcacttg tagaaattga ggctcaaaaa atagcaaaag aaagaaatgc atttagagca
     1381 gaccaattta atttagaagc ttgtaatcat gcacatgaaa agtatacagc agaagaaatg
     1441 tgggaacaat tggatggaaa tattgatgta tttgtagatt ttcttggaag tggtagtact
     1501 tttgcaggat gttcaaaaac acttaaaaaa cataattctt ctatcaaatg ttatgttgtt
     1561 gaacctgaaa cagcagcaat ttattcaggt aaagaaataa ctgaccaagg gcataaaata
     1621 caaggtggag gatattctat ggatttgcca ttagtagaca agaaacttat tgatggttat
     1681 attcaagtta cagacgaaga ggcaactgat gtagcaagaa aattagctaa attagaaggt
     1741 gtatttgcag gattttcttc aggggcaaat gtatgtgcag caattaagtt gttaaagagc
     1801 agtgaaaaag gaaaaaatat agttttaact cttaatgata gtggtttaaa atatttaagt
     1861 actgatttat atgaagaatt gtaataatag cacaatagtt atgcaatgtt ttttaaatta
     1921 agtatataga tttaaattat aaaaagtatc tcaatgtgat ttttgaaagt tataaaggag
     1981 atactttttt attttgtaaa tatggagtat cctacttata agtattaagc tatatagata
     2041 ttgatgatta gctatgcaat aaaaaagagt atttgttttg catttataca tcataacttg
     2101 gaataataca tattaatgtt ctaaatgtat ttaaaacata tgataaaatt agaacaaaca
     2161 gatacaagga gataaacatg caaaagagaa ttttaagaaa ttttatattt acgggtaaaa
     2221 atattttaag taaacttgtt tttataataa aatataattt tagtacatta attttatttg
     2281 aattatttca taaaggatta gccttatttt ttatatggcc atctattaga tatatatttg
     2341 atattttaat aaaaagttta catatagtct acctaaatat ggataatttt ataaaagttt
     2401 tgaccaatcc catgtcaata gttctgataa tactatcagt tataatacta gctttttatg
     2461 cattttttga atttacctct gtaattattt gttttaataa atcgataaaa tatgaaaaaa
     2521 tagggttatt tgaactcttt aaaatatcat ttgagaactc tataaaattg ttatatccta
     2581 aaaatatact attatttata tttgtgatat taataattcc attagtaaat acagttttaa
     2641 tttcagggtt tataggaaaa atacaaatac c
//
LOCUS       DAELNY010000276         2666 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.279, whole genome shotgun sequence.
ACCESSION   DAELNY010000276 DAELNY010000000
VERSION     DAELNY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.279"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            155..823
                     /locus_tag="KPE55_003562"
     CDS             155..823
                     /locus_tag="KPE55_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494591.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            825..1310
                     /locus_tag="KPE55_003563"
     CDS             825..1310
                     /locus_tag="KPE55_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0494592.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1371..2492
                     /locus_tag="KPE55_003564"
     CDS             1371..2492
                     /locus_tag="KPE55_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG0494593.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
BASE COUNT          999 a          297 c          533 g          837 t
ORIGIN      
        1 ttgaaaagta agtaattata agctatttaa tcactggaat aaatttacaa taaaaaaata
       61 aatacaaagg ctttatcatt aaggtattgg cataagtttt gcttattaaa caagtatact
      121 aataaaattc atttaaacag gagggagaat ttatatgagt aaattagaat caacaacaga
      181 aaaagttgag gtaaaatggt atgggtatgt agcattaata ttgggagcat tatttttttc
      241 aggaatattt aaagatgcac caggaccatt aaaagtattg gattttaaca atgtattagg
      301 ttcttttgga acattgggaa caattaatga tggtgtaggt acattagctg caaactttag
      361 aggtgatgga ggtactggcc ctcgagatgg atggctatat gcattgacat taataccatc
      421 agttatgttt gcacttggag tagttagagt tatagaccac ttagatggaa tgaaggcagc
      481 tcaaaagctt cttagtccac tattaaagcc attattaggg ttacctggat ttgcaggact
      541 aactttgata gctagtttac agtctacaga tgcagctgca tctatgacta aagaacttaa
      601 agatgatggg tatatagatg aaaaacaaaa agctgtattt tgtgcatttc aattttcagg
      661 tgcgtctgca attacgaatt tctttgcttc tggagcagca ctatttccat ttataggaga
      721 tgtaccaata tttataccac ttgctttaat attaattatg aagtttgttg gggcgaactt
      781 acttagatta tatttaaata aatttgacaa gggggaagct taacatgagt gaggtagcaa
      841 aaaaagaagt aaaagttaaa aaaggaaatg tgatagatgc gtttattgga ggagctagaa
      901 atggattcca aataagtaca aattcaatgg ctccaaatgt catcttcggt tttgctataa
      961 tttctgtgtt caatttaaca ggtcttttgg atgtaatagg gacaatattt acacctgtaa
     1021 tgagtatctt tggacttcca ggtgttgcag caacagctat aatgacaata tttatatcaa
     1081 tgggaggagc atctggagta atcgcaggtt tatttacagc aggtcaaata agtcaagcac
     1141 atgttgcaat tctattgcca atgttattgc tttcaggagc gcagatacaa tttatgggga
     1201 gacttttagg aacagctgag ttaaaaacta aatattatcc acatatgttt gttatagcaa
     1261 ctttaaatgg ttgtattgca atgcttataa tgaattattt cgtagtatag gtaattataa
     1321 taaagattta atataaatgg aaagtttaaa ttttaaggag gagctagatt atggaaacta
     1381 acaaagacta tattgagtat gtacataaag tgtatgatta tctacacact ataccagaaa
     1441 aaggatttga agaatataag acatcagagt ttgttttaaa agagcttgaa aaaatggggt
     1501 ttgaaaatat taataaagga attgcaggaa ctggtattat agtaactata gattctaaaa
     1561 aagaaggacc agtatttgct ataagagcag atatggatgc attggaattt atcatagatg
     1621 gagaaaagaa aatgatacat gcttgtggtc atgatggtca tacttctatg cttttaacag
     1681 ctgcaaagtt aataaaagag agaaatttag taaaaagagg taagttaaaa atactttttc
     1741 aaccaggtga agaaaaatta tatggagcat taagagtaat tgattctggt ttgttggatg
     1801 atgtagaaga aatggtaggg atgcatttaa gacctggaca agattgtagg ttgggaaagg
     1861 cgatacctgc aatgtaccat tgtgctagct gtataatgga atttacgatt aaaggtatca
     1921 cttgtcatgg ttctaaacca catttaggag taaatgcagt agatgctgtc tgtttagcag
     1981 tgaatgcaat aaatgcaata agagtaaatc cagctatacc aaattctata aaagtaacaa
     2041 atatatcagt taagggagaa acttataata atataccaga tgaagcattt atggctttag
     2101 atataagggc agaaagtaat gatataatag aagaattaaa agaaaaagca atacatgctg
     2161 taaaaactgc tgtaagttct ataaatgcag aagctgtact aatttccaaa gatggagtac
     2221 cagcagcaga atttgatgaa gaacttgtaa acttggctga agaaactata aaagatgtat
     2281 tgggagctga tgcatctata ggtatattta aaaattctgg tggagaagat ttccattatt
     2341 atacacaaaa actaaaatgt aaaactacat atattggatt aggagctgat gctacaccag
     2401 gatttcataa tccaaatgta acatttgata cgaaagcttt agaatatggt gtagatatat
     2461 ggtgtagatt ggttgaaaag agattgggat aacatccaaa ctaataattc tacatttaaa
     2521 attataatta tagaagttat agaaaaagat tgcttatgtt aattagaaaa taattaatat
     2581 gagcaatctt attttgatat aatgaaactg attttggaat attgaaaaaa tatgatggaa
     2641 aagtaattat attttaaatc tattgg
//
LOCUS       DAELNY010000277         2644 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.280, whole genome shotgun sequence.
ACCESSION   DAELNY010000277 DAELNY010000000
VERSION     DAELNY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.280"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..1316
                     /locus_tag="KPE55_003565"
     CDS             117..1316
                     /locus_tag="KPE55_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494594.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIETIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1325..2137
                     /locus_tag="KPE55_003566"
     CDS             1325..2137
                     /locus_tag="KPE55_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494595.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVKVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2148..>2644
                     /locus_tag="KPE55_003567"
     CDS             2148..>2644
                     /locus_tag="KPE55_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494596.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKT"
BASE COUNT         1127 a          211 c          396 g          910 t
ORIGIN      
        1 tttcaattta acaatttagt agcagggtta ttaagttttt taattataat gtttattcta
       61 aatggaatat cttatttgct aattaaaaag aaagtatctc tttaaggagg ggtggtatga
      121 aattaaaatc taaaatatct atacttatta tatttttgac tttagcaata tattttagta
      181 taggtttatt aaataatgaa gataatttaa agataaaaac tataaaaggt gactctaatg
      241 aattagacca tataaacata aattttggat atgctttgaa tggatatata aattcatatt
      301 atacaataaa tggaagtaac aaaaccaaaa agactgaatt aataaaacta caaaaggata
      361 gtaagaacac aaataattta attgatgtat ctagtggttc agatttaatt gcacatgttg
      421 gatttgaaat gaatgaggag tcaaaagata taaactttgt aatagatgag aaaaaagaag
      481 atttagaaaa aggtgtattt ccatcagaag gaagtgataa agaaattgaa tatgaaaaat
      541 tagatatgaa taattttaca aggacaagat ataaagtaga taaaagtaca gatgattatt
      601 atgcaaatat actagcatca aaaagatata aaaaatactt atatgtatct gtactttatg
      661 ctacaagtga ttcatcagaa aataacaaag aatattcaag tactatagat ataataaaaa
      721 ttaatccaga aaataaaaaa atcaagacta taaaaagtat aaatttaagt aattattttt
      781 ccgaaaaata ccaaaacata gagaccatag gtgtaagtta tgctagcaaa tatgaggata
      841 agatgtatat tttagtaaat gtactaaata caaacatagg tgataatagt aattctaaat
      901 ttcatttact tgagtataat cttgaagaaa ataaggaaag cataatggaa ttaaaaactg
      961 atagcaaaat ggctttagca aaagcaaagt tagaagctaa taaattatat atggtaatgt
     1021 cgaatgatta tgataatagt tcaattacag agttaaattt acttacctat aatatattag
     1081 ataaaagctt agatgaagat aaatatgttt tagataatag agaaaaaaga ggaattagaa
     1141 gtattgaaat tgattcaaat aaaatcgttg catatacatc aaggctaaac tctatattat
     1201 cttgggataa taaatttatg ctatatataa taaataaaaa ttctaaggaa attgtatatg
     1261 aaggagaatt acaatttaac agttcaaata gtatgttaga atttgaaata aagtaggtga
     1321 gttgatgaaa gatttaataa gattattcga atttgaacta aaaagaaata taaagaatta
     1381 tatatttata ataatttgtt gttgttcttt tgtaatttta aacatagtaa aaaatttaaa
     1441 tgactataat tacataatag aaaatgcagt taaaagtgaa cagttaacaa aaatcggaaa
     1501 tacgatagaa acggtaaagg ttacaggttt ctcaagcttt agaaatataa tgggttctac
     1561 agaatcgtgg tttatgtttg gtataattgc atgtatttgc tatgcttttt ttatttggta
     1621 tagagacttt aatggtagaa gtaaatctat atatacactt ataatgctac ctaaaaatag
     1681 aatcaatatt tatatatcta aattattaaa tatattattt ctagtgtata gctatacagt
     1741 agtacttaca atatctttgt ttattgcaag taagttgttg cctagacata tgcttggtaa
     1801 tgtcacaaac tatggttttg tacaagaaac aatatatgag ttaaaaatgt tacttccata
     1861 tagctttgaa atactatttg tagagtatat atttttattg ataggatttg tatctgtagt
     1921 atttacatca attcttataa ataaatctat ttataaagta agcactttaa taagttttct
     1981 atttttagtt atagaaattt tagtgtttat aatttctata gaatttatta taccctacga
     2041 atacagtgat gtatttactg ttctatattc aagtataaac atatttgtat gtagtcttat
     2101 atctaataaa cttttgaagc aaaagattga cttttaggag ggatattatg aaagcaaaaa
     2161 atataggaaa atatacaata tgttttataa tagtatgttt tttaatgatt actatgacct
     2221 taatattgag ttattttaat aaggataaac tatctgtaaa aactttaaaa ggcaatgtaa
     2281 aagatatagg gagagtaagt gtagtatata cacctaatat aagcaaattt tcttatgagg
     2341 aggtaattat aagtaaaaat ggagtaagaa ataatacaaa cccaaaatca agaattagaa
     2401 atgattattt aaatattgac aaaaaataca gtgactttgt taatgataaa ggaaaagaat
     2461 ttatatataa agatgatttg aatataggag aagcgtatac ctctgttaag tctgatttag
     2521 tacaaggaat gaatgcttta aatgattgta taaaggtttc aaataaaaat ttaaaaagtg
     2581 gggtagaaga aaattttgat attaatttaa ataagtctga ttttaaagaa aatagttgga
     2641 aaac
//
LOCUS       DAELNY010000278         2626 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.281, whole genome shotgun sequence.
ACCESSION   DAELNY010000278 DAELNY010000000
VERSION     DAELNY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.281"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            199..1704
                     /locus_tag="KPE55_003568"
     CDS             199..1704
                     /locus_tag="KPE55_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0494597.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1818..2516
                     /locus_tag="KPE55_003569"
     CDS             1818..2516
                     /locus_tag="KPE55_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0494598.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
BASE COUNT         1098 a          251 c          399 g          878 t
ORIGIN      
        1 tagaagtaat aaaaaaatag ttaatataaa agtgatacaa taataataga agtaataaaa
       61 aaaaataatt aatataaaaa tgtgtacaaa ctaatgaata ttgatatagt aagctatcat
      121 atgaaagagt atgtacacat tttttttact aatttttcac aatatgaaca taaataattt
      181 acaggaaagg aatcttttat gaaaattcaa aatactgagt ggggatatat tgaatggaaa
      241 catacttatg atgaaaataa tccaaaacaa gctatgaata tatacattgc agtaactatg
      301 cctggtaaga aacattttaa tcatgttcat tatggtcaag agcagatgat atatatttta
      361 gagggagagg gtttgtatat tataaatgga gtttggaagc cattttatca aggtatgatt
      421 ttttatatag aatctggtag cacacatgaa actataaata caggtgatag agaaataaaa
      481 gaacttattg tttcaaataa tgtagatgat gtaggagaga gtgaagttat agatattaat
      541 cctaataatt atttaaagaa gaccttgata aattatagtg aaagtacatt aaatttgtat
      601 gcagcagtag aatcaattcg tggtcaattt atagacccgt ttaaaattcc attaattata
      661 tatgatgatt catggaatat agttttaaaa aatccttatt ttccactttt ttgttttgaa
      721 aaatgtaatc ctatgaaatt tcctcaaaat tgtgattgta tgaatcaaaa aagtagcaat
      781 caatttgtat gtgaatatgg gataactata tataatatac caatactgta caaaagcaat
      841 agcataggtg ttataagagg agggtatgta ctattatctg atttgaattt agatacagag
      901 cataataatt tatatgatat tccagaggga gctgctagaa gtataaaacg cctcctgaaa
      961 caaatatcta aaaatataat aaatttttgc tcttttaatg atataagaaa ggatttacaa
     1021 gaaaaagaga agactatagc aagaacatat cattatggag agcaacttga aatgaattta
     1081 aaagtagctc aagatatggt tactaattta agaataaatc atcacttttt atttaataca
     1141 ttaaattcaa tggcaagcat agcgctagat gatggtagtt atgatttgta tagtgcaata
     1201 atagatttat caagaatgtt tagatataca atgaggtctg atttaagatt tgtagaatta
     1261 gaatctgaaa tattatacat taaaaattat ttgaatttac agaaattacg ttatggagat
     1321 gcacttaaag taaaatatct tattccagag aagttatata acctttctgt gccttttaac
     1381 tttattcaac ctattgtaga gaatgcattt acacatggat ttagagatat tgacacagaa
     1441 aagcgaattg aaataattgc aagattagat tctcagtatg ctattataga gattcataat
     1501 aatggaacta tcttagatgg aaataatata gacaaaataa aggcaggtat tcgtagtaat
     1561 aatggtcatg gtttgtcttt aatatataca aaattcacat ctgcttatgg aaataatttt
     1621 gatatggata taaagtcgag tgataatgaa ggaacttaca taatgattaa aattcctatt
     1681 gaaaattata aggaatatgt atagaacttg tgaaaaatta ataagtttta taaatgttaa
     1741 gaaatatatt agatgttttt atgttgtttt atatatctta cttttttata ttattaagct
     1801 aaaagggagg tattctcatg agaagagcac taataataga tgatgaagaa gctgttctaa
     1861 aaataataac acattttata gaaatgaaag atatgccaat aaaaattgtt ggtaaagcaa
     1921 catctggtga tgaagcagta gataaaatca ttggtttaga gccagacata gtttttatag
     1981 atattcaaat gccaatatac aacgggttag aagtaataga aaaaacatct catttatcta
     2041 aaaaaataaa ctttgtagta ataactgcat ttaactattt tgaatatgct caaaaagctt
     2101 tgagattaaa tgtaaaagat atactcctta aaccattgga tatgaaagaa tttactaagt
     2161 ctgtagaaaa aattattgga tatcaatata ccaataatga tttattaaat gagatactgg
     2221 aatatataaa tctaaactat agcaatgact taaagttaaa tgattgtgct agattatttt
     2281 taaccaatcc atctaatata tctaggattt ttaaaagtaa tttaaataca acttttatat
     2341 catatctaaa tcatataaga atagaaaaat ctattgagtt acttgaaaat actacaaaaa
     2401 gtataaatga gattgcagaa atagttggat ataatagcct aaataacttt tataaaaact
     2461 ttaagataga aaaaggaatg actccaaaag tttataaact gaacactaaa aattgaaatt
     2521 gataaataaa aagtcaatat agataattgt ttgagtgtgt ctgaataagt atactttaat
     2581 tatgataaaa attaaagcgc tttaaacata tttaataata ttaaac
//
LOCUS       DAELNY010000279         2560 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.282, whole genome shotgun sequence.
ACCESSION   DAELNY010000279 DAELNY010000000
VERSION     DAELNY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.282"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..276
                     /locus_tag="KPE55_003570"
     CDS             106..276
                     /locus_tag="KPE55_003570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494599.1"
                     /translation="MLTNDAFIASYTHRNLFIKYRKIFIELARRTDSIKESFNRIIEE
                     IAIIRGDDNNVF"
     gene            266..1111
                     /locus_tag="KPE55_003571"
     CDS             266..1111
                     /locus_tag="KPE55_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494600.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLVICIFYSFNSMESQKALM
                     EMSTSKKPDLEMFKNMISYISVFVSAILCTLILYINNLLIKKRNKKLGIYMTLGMGKI
                     EMSKILVFETIIVGIVSLVSGLILGLLVSQGLLILLLKLFEFNMSEYSFVISIPAIIK
                     TIVYFGIIFLLVMIFNIYLISKYKMIDLLIVERKTEDIKFKNPFVYMITFVSCFIFLF
                     TVYNTTNHIIINLIDDKQLNSKSKGVEINEFTYQDWQQFCDIKYYGKKGLISMTLKEY
                     IEKYK"
     gene            1366..2133
                     /locus_tag="KPE55_003572"
     CDS             1366..2133
                     /locus_tag="KPE55_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494601.1"
                     /translation="MKNILSVEKIEKYYGNKDNITKAIDNISFKVDEGEFVGIMGPSG
                     SGKTTLLNCISTIDNVTIGKIIINNNDITRLKSKNLDKFRQNELGFIFQDFNLLDTLT
                     AYENIALALTIKGEKPSKIDSKIRDVATYLEIEKVIDKYPYQMSGGQKQRVASARAIV
                     TNPSLILADEPTGALDSKSARLLLERFESLNQDLKATILMVTHDAFTASYAHRILFIK
                     DGKIFTELVRGADSRKEFFNRIIEVTTLIGGDDNDVF"
     gene            2123..>2560
                     /locus_tag="KPE55_003573"
     CDS             2123..>2560
                     /locus_tag="KPE55_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0494602.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLAICIFYSFNSIESQKALM
                     EMNASTKSHVEMLKNMISYMSVFVSVILCSLILYANNFLVKKRNKELGIYMTLGMEKV
                     KISKILVFETIIVGIVSLVSGLILGLLVSQGLSILLLKLFEFNM"
BASE COUNT         1073 a          238 c          377 g          872 t
ORIGIN      
        1 cattaacaat taaagggaaa acaatataaa tttgattcaa aagcatcaag attattactt
       61 gagagttttg aaagtttaaa tcaagattta aaaacaacaa ttttaatgct aactaatgat
      121 gcttttatag ctagttatac tcatagaaac ttatttataa aatacagaaa aatatttata
      181 gagttagcaa gacgaactga ttcaataaaa gagtctttta atagaataat tgaagaaata
      241 gctataatca gaggagatga taacaatgta ttctaaaata gcgattaaaa atgttagaaa
      301 aagctttaag gattatagta tatatttttt aactttaaca ttagtaattt gtattttcta
      361 tagttttaac tctatggaat ctcaaaaagc tttaatggaa atgagtactt caaaaaagcc
      421 tgatttagaa atgtttaaga atatgatttc atatatatct gtgttcgtat cagctatttt
      481 atgtacttta attctttata ttaataactt attaatcaaa aaaagaaata agaaacttgg
      541 aatttatatg actttaggaa tgggaaaaat tgaaatgtcg aaaatattag tttttgaaac
      601 tataatagtt ggaatagtct ctttggtaag tgggctaata cttggattat tagtatctca
      661 agggttatta atactattat taaagttatt tgaatttaat atgagtgaat atagctttgt
      721 aatatcaata ccagcaatta ttaaaactat tgtctatttt ggaataatat ttttacttgt
      781 aatgatattt aatatctatt taatttctaa atacaaaatg atagatttat taattgtgga
      841 aagaaaaaca gaggatataa aatttaaaaa tccatttgta tatatgatta catttgtatc
      901 atgctttatt ttcttattta ctgtatataa cactacaaat catatcataa ttaatctaat
      961 agatgataaa caattaaact ccaagtctaa aggtgtagaa ataaatgaat ttacatatca
     1021 agattggcaa caattttgtg atataaagta ttatggaaag aaaggtttaa tatcaatgac
     1081 tttaaaagaa tatatagaaa aatacaagta gaataaaata aattttagat ggaaggcatt
     1141 gccctctata tttttgtgtt ttctagaatt gagtaaattt tgttactcta gcaaagtata
     1201 aaagaaagaa tgctggtctt gagtatttta tattagtaat tattagggaa taatatataa
     1261 ttactacaaa aatgaaacat taatgaaatg taaatcaatt taaaaaaatt aaataaaaaa
     1321 ttacaataga gatataagaa aattaattta aaggagaaat agaacatgaa gaatatatta
     1381 agtgttgaaa aaatagaaaa atattatgga aacaaagata atattacaaa agcaatagat
     1441 aatataagct ttaaagttga tgaaggagaa tttgttggga taatgggtcc aagtggtagt
     1501 gggaagacaa cacttcttaa ctgtatatca acaatagaca atgtaacaat aggtaaaata
     1561 ataataaata ataatgatat tacaagatta aaatctaaaa acttagataa gttcagacaa
     1621 aatgaattag gatttatatt ccaagatttt aatcttttag atactttaac agcttatgaa
     1681 aatatagcat tggcattaac aattaaagga gaaaaaccat ctaaaattga ttcaaaaata
     1741 agagatgtag ctacatactt agaaatagaa aaagtgatag ataaatatcc ttatcaaatg
     1801 tcaggaggac aaaagcaaag agtagcatca gctagagcaa tagttactaa tccatcattg
     1861 attttggcag atgaacctac aggagcactt gactcaaaat cagcaagatt attacttgag
     1921 aggtttgaaa gtctaaatca agacttaaaa gcaacaattt taatggtaac tcatgatgct
     1981 tttacagcta gttatgccca tagaatccta tttataaaag acggaaaaat atttacagaa
     2041 ttagtaagag gagctgattc aagaaaagag ttttttaata gaataattga agtaacaact
     2101 ctaataggag gagatgataa cgatgtattc taaaatagca attaaaaatg ttagaaaaag
     2161 ctttaaggat tatagcatat attttttgac tttaacatta gcaatttgta ttttctatag
     2221 ttttaactct atagaatctc aaaaagcttt aatggaaatg aatgcttcaa caaaatctca
     2281 tgtagaaatg cttaagaata tgatttcata catgtctgta tttgtatcag ttattttatg
     2341 cagtttaatt ctttatgcta ataacttctt agtcaaaaaa aggaataagg aacttggaat
     2401 ttatatgact ttagggatgg aaaaagttaa aatatcgaaa atattagttt ttgaaactat
     2461 aatagttgga atagtttctt tagtaagtgg actaatactt gggttattag tatctcaagg
     2521 gttatcaata ttattattaa agttatttga atttaatatg
//
LOCUS       DAELNY010000280         2503 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.283, whole genome shotgun sequence.
ACCESSION   DAELNY010000280 DAELNY010000000
VERSION     DAELNY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.283"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            185..442
                     /locus_tag="KPE55_003574"
     CDS             185..442
                     /locus_tag="KPE55_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0494603.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            911..2149
                     /locus_tag="KPE55_003575"
     CDS             911..2149
                     /locus_tag="KPE55_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0494604.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1088 a          201 c          401 g          813 t
ORIGIN      
        1 ctttattaaa gctaacaaag ttataagtta tgagaaattt tattaaagtt aatacaaaaa
       61 ttaattttaa agctaagaaa ttttttatta gattcaataa aactaataaa tatagtttag
      121 tcaataatgt attaaataaa aactttgtta agtctataaa aatatcttaa aggaggtgta
      181 acttatgaag ataaaagtgg aggatatacc agttcaattt catgcaatgg tagaaataat
      241 tggaattgat aagtttatag aggttgccaa gttgtatgga ggaacaaata cttatatacc
      301 tacgtataag gggcttttta gatatgctag aaatagggat atagtaaaac aatttaatgg
      361 agtcaaccac aatgaattgg ctattagata taatatgtgt gtaagcaata ttaagcgaat
      421 actcaatgaa aataatgttt aaatataata aaataatgtt taaacataat gaaaatagcc
      481 ttgttaaacg taagagtaaa gacaaagaag atgttttata aagttttttt agacaaatta
      541 actaaattta ctacttatta atttaaatac attgtatatt ctataaaaaa atttgttaaa
      601 tactggagat atattgagaa tacaaagaaa atggtcgaaa atatttgttg gctaaaaaat
      661 agataaaatg atacaattgc ttgtataaag gggaaaaata aacaaataaa ctatataaat
      721 aaagaaatag caattaatat gacataatat atttataaaa ttaagaatat aaatagatat
      781 tttaaggtat tagttgttaa attaggaaat attaaaaaaa atatatattt atatttaaac
      841 tattttgtta tttatataaa aatacttatc tagttactag caattattag tagaaatggg
      901 ggaaatagag atgaataagg gattttacaa attaaattca gtggtattga actttgatat
      961 taagaatgat gttaacaaga taagatttaa tcattatata atgagaaaag aaaacataca
     1021 aagacttcaa ggaactctgc caaacggaca attttatatg acagtgagaa gagctgctac
     1081 agatttacaa ctttctattt ctactataag taggcttgta aaagagtttt tggatttagg
     1141 gataataaga ttagtgtcta agggtgtaaa aggtaatcgt tctgtatata gttacgtaag
     1201 tatagagttt aatggaagtt tatttgatga tagtaaatat atggaacttg actgtgtatg
     1261 taatgatgaa tttattgata atagtggcat agagtttgat tgtgatagca aagataattc
     1321 ttttaatgga gatataaatg tagaaactgt gcattgcatt aatgagtatt tgtctgatga
     1381 tgatgtagag cataattata acaatttcca taataaagat actataaagg ataaagccat
     1441 ttctaatatt agcactatca aaaaatatat tgatgaatct tttgataaaa ataaatcaga
     1501 aaattctaac ttacaacagg agtcaataaa tttcaacttt aatgatacat gcaatgaaat
     1561 agataaaaga acgaataata gtatagaaaa taatcaaaat aaaaggccta tgagattgag
     1621 tgacatcagt gttttaagtg ataaaggtat gacaaataaa aagaattact ttaatcataa
     1681 tcatggtata caaaaaggga ctaaatctgt aacaaagaaa aaagaatcct taaataaatt
     1741 taataaaaaa aatatacagc aaagcattat aaatatgcta aataaaaaat caggtaagaa
     1801 ttttaaaatc gattctccaa taagcataaa acttatcaat gaaaggctaa aggaaggcta
     1861 tgtcttagaa gatttctaca aagttattga ggtaaaagtg gctaagtgga aaaataccat
     1921 aatggagatg tatataagac cagaaacctt gtttagctat aagtttgaat cctatgtaaa
     1981 tgaaaacata tctatgaaaa gtcaaagtaa tgttcaccca tgtaaagaag gcaatagtaa
     2041 tgatagcagt aattatatac attctgatta tacaacatat gatggcagtg tgcgtgagag
     2101 aaaaaaatat gagaatgtaa gatactggaa tcctatatgt gagatttaat tagtttaaga
     2161 atgtatttat gataaataag atattaatag agaatagtga gctatagcaa atgatatatc
     2221 aatagttggt agtaagttat attaagtaag atattactat caggtagtga attatattga
     2281 acgatatatc aataattggt agtaaattat attaagtaag atattactat caggtagtga
     2341 attatattga acgatatatt aataattggc agtaagttat atcaaataag atattactat
     2401 agagtaatga attatattga acgatatatt aataattggc agtaaattat attaaataag
     2461 atattactat agagtaatga attatattgg acgatatatc aat
//
LOCUS       DAELNY010000281         2461 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.284, whole genome shotgun sequence.
ACCESSION   DAELNY010000281 DAELNY010000000
VERSION     DAELNY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.284"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(176..2269)
                     /locus_tag="KPE55_003576"
     CDS             complement(176..2269)
                     /locus_tag="KPE55_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0494605.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKVLQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT          840 a          405 c          235 g          981 t
ORIGIN      
        1 tttttattat agttatccat ttttaaaata ctttaatcat atactactcc caatttaaca
       61 tccattgaaa tgctattttc ttatattaca atttataatt gatatatctt caatataatt
      121 atttaatgta tttttgattt taaagctttt cagtgatatt attgataaat tagaattaat
      181 tatctagctt taaaaggtca aagatttctg ttgctgtctg cggttttcca aataaatatc
      241 cttgtatgta atctggcttt atttgtttaa ctaaatcata ctcttcttgt gtttctacac
      301 cttctaaaca tacttttatt cctacgtcat gacaaattgc aacaataaaa tgtataaatg
      361 tagcatcaaa cttactcttt aatatatctt taacaaatac tctatcaatt ttcactatat
      421 ctattggaga aaattttaaa acttctagag acgagtatcc cgtaccaaaa tcatccatag
      481 caatataaat tcctaaatct tgtaaaacct taaatttaaa ctgtaaaata ctcatatttt
      541 gtactgtatg actttctgtc aattctaaaa caagattttg ataaggaaaa ttttcatcat
      601 ctataatttt tactatatct tcaataaatg tgtcttctaa tatttgaaca gctgaaacgt
      661 ttacactcac tttaaaaaaa ggatagtaat caatccattt cctaaaagta cgtaatgcca
      721 ttcgaagcac ccaagcccct acaatattta tcatatcatt ttcttctaat attggaataa
      781 attccaaagg agaaattgct ttgcatttgt cattagtcca ccttaaaaga acttctacac
      841 ctattatttt atgactttct gtatctactt gtggctggaa tcgaagacta aaccccctaa
      901 aatcatcatt aattgatgcc tttaaatccc gcatcatttc taatgaatac attttatttt
      961 ttaatatttc ttgggagaaa aaaacaagcc tgtttttacc atgctctttc gcatattgta
     1021 aagagtaact agcacactta taaagttctt ttactgtatc tccatgcttt ggatatatta
     1081 cacaaccagc agaaattgtt atatttaatc catattttct ccatagttga agatgtataa
     1141 tcatattctg tatttgatta tatagagtta gaatttcatt ttcttcaaca ttatcaacaa
     1201 gaatacccat ttcgtcccca tcaagtttat aaagttcagc attatctggt aaaatagatt
     1261 gtatagactg tgctagtgtt tttattatat tatctccaaa atccctatca tacatctcat
     1321 taacattttt aaaatcgtca atatttaata atactatgca taatttttca atcataggat
     1381 ttgaaatttt atcttcaaat acactcataa attcatgata attcagtaac tgtgtcaatg
     1441 aatcaatctt attttgttta cctaattgcg tcattattcc tgcaaaaata cttggttctc
     1501 caaattcatc acgcattagt tgacctctac atcttaacca tatatattct ccactacgat
     1561 ttttagccct aaattccact gtatgataat ccatctggtt tttacctatt tcagtgtttg
     1621 acttataaaa acgattccag tcttctggat gtacaatttt tttccagtaa acaagcggat
     1681 tttcaacaat ttctcctggt aagtcaaata attcaacttg tgatggtgaa tatcgaaata
     1741 cgcctgtttt catattgcaa atgtatatat attcatctgt acctctaatc aaagcatcat
     1801 aaagcaatgt agaattataa tcaaaattgt ctcttgaaaa attaaaaact cctttttctt
     1861 ctttattatt tgatatttca tgaaattcat taatcttaat tggtttattt aagcttgttg
     1921 agtttgcaat atctattgat tgatgcatat gaacaactgt gccatcttgc catgtaatta
     1981 aactatcata attttcaaaa acacgattta acttattaca tttttcatac cactttatta
     2041 atactccttt tttatcattc tttaataatt ctaaaacttt acaaaaacta caagtagaat
     2101 ttttttcagg atataatact tgccaacaaa cttttccttc tggatctaaa atattatatt
     2161 cttctttcat ttttttgttc ataaaaataa tttcattagt atgaatattt gtaacatata
     2221 tattaatctg tagttgattt aatataactt caaaattatg tttgttcata tacttatctc
     2281 ctccatagtt actgctcttt ataacaacat tgttaaaatt attcttttta gaagatttac
     2341 ttataactta tacatataca ctaaatacct taatttaatt agaaagttta ttacttcata
     2401 ttttaaatta taattgataa aaaaaaatac agcaatatag tattaccaat ttaaaaaaaa
     2461 t
//
LOCUS       DAELNY010000282         2402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.285, whole genome shotgun sequence.
ACCESSION   DAELNY010000282 DAELNY010000000
VERSION     DAELNY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.285"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1259
                     /locus_tag="KPE55_003577"
     CDS             <1..1259
                     /locus_tag="KPE55_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0494606.1"
                     /translation="IFSIHEITLNTENFKRARKESVNLIKGKIIRTIIYSLILSLVVA
                     IIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIAFEEYFRLILSIFIFILDIGFI
                     SATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFS
                     FYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTT
                     GVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEKIKYSKGKVKLLIE
                     IKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAY
                     GDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIIT
                     DNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1581..2165
                     /locus_tag="KPE55_003578"
     CDS             1581..2165
                     /locus_tag="KPE55_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0494607.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          943 a          230 c          390 g          839 t
ORIGIN      
        1 ggatttttag cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag
       61 aaagtgttaa tcttataaag ggaaagatta taagaacaat tatttattca ttaatattat
      121 ctttagtagt agctattatt ggatatgcga tttatcatag tggaattata tttataggca
      181 tatggactaa atattgtact gatatggaat ttttgaagaa tgtatttata aatagatgta
      241 ttgcatttga agaatatttt agattgattt tgtcaatatt catttttata ttggacattg
      301 gatttatctc agcaacttat tatcaatata aaggagtaag tatttctaaa aataatatta
      361 aaaaagagaa aaaatctata tcaaaaattg tttttaattt agtattaata ttattaattt
      421 cttatataga gtcagttgca ttttcttcac atataaatgg tatgtttaat acatcatttt
      481 cattttatac aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt
      541 tggcagcaat agaagaagct tcacatgcta aagctgatta tgcagaaata gatgttcaag
      601 aaactaaaga tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag
      661 tagataaaaa tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct
      721 tttattatta tggaaaaggt tattatgatg aaaagtttgt tggggaaaag ataccaacac
      781 tcaaagaaaa gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta
      841 atggacatga aaagggagac gtagtaaaaa aagttgttca gttaattaaa gaaaataatt
      901 ttgaaaatca atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac
      961 tggatgagaa tttactgaca tgctatttta tggcaattgc atatggtgat ttttcaaagt
     1021 taaattatgt tgatatatat ggaattgaat caacttttgt taataaaaca gtagtagaaa
     1081 agattcataa aatgaacaaa aaaatcttcg tttggactgt taatagtgac agtttaattg
     1141 agaaaatgct tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga
     1201 gtgcaattta ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa
     1261 tattctataa ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt
     1321 taacataaat aaggttgtta aattaaggtt attcttataa aaattgtgct tatcagctat
     1381 aaacaaggaa taaaagtgaa aaaattgctc tgagattatt acagttaaaa gagttttaag
     1441 atgacagaga gatagattgt caactgtata tttaataaat cttgttattt attaaatttt
     1501 atataagtaa aatatataaa ttaataaatc ttatgctaga atatatatga aaaacaatat
     1561 aaatttggag gattaaaaac atgacagagc gagaaaaaat gttggcagga gaactttacg
     1621 attgtggtga tattgagttg ttaactcaat ggcataaagc aaaagaccta gtaagagatt
     1681 ataaccaaac agattcctcg gatttggaaa aaaaagaaaa aatattaaat gagttacttg
     1741 gtggtaaggg taaaaactta tggattactg caccatatta tgtagattat ggaaataata
     1801 tttattttgg aaataattgt gaagtcaata tgaattgtac gtttttagat gataataaaa
     1861 ttatcattgg ggataatgca ctaatcgcac caaacgtaca aatatatact gcatttcatc
     1921 caactaatgc tggtgataga tttggtaatg ccaaagaaga tggttcattt gaattttgta
     1981 aaactcaaac agctccagtt actattggag ataatgtttg gattggcggg ggtgctatta
     2041 tcatgccagg tgttactatt ggagataatg tagttatagg tgcaggaagt attgttacaa
     2101 aagatattcc aagtaatatg attgcatacg gaaatccatg ccgtattatt cgagaaaata
     2161 aataaagtta aattttcttg caaaatctca aaaaatacct ttcttacttt atacataggg
     2221 tattttctaa tactttcaca tccatattca tttgttaaat ttttatggaa tgtagttttt
     2281 atcatctcta tcttacattt tcactatttt tttataaaag tcattccttt atagatttat
     2341 ttataatagt atatattcta tttgatgttt gaataagcac ttatttttaa gtaaaatact
     2401 aa
//
LOCUS       DAELNY010000283         2393 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.286, whole genome shotgun sequence.
ACCESSION   DAELNY010000283 DAELNY010000000
VERSION     DAELNY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.286"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..463)
                     /locus_tag="KPE55_003579"
     CDS             complement(<1..463)
                     /locus_tag="KPE55_003579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494608.1"
                     /translation="MQLIINKEYFKQNYMIDKGIARHNSVKNIVILPYTTTTKEKSKL
                     KEKVERFESCVGEFCRLVYNKKMNIKERQLKDIKLLIKDINVNSSDDSVQLESIIREL
                     FLDEEENMYIFHPKLLNYIHMPSDSYYKKLSKFLYDVLLNSTDNITIKNKIN"
     gene            complement(451..2259)
                     /gene="dptF"
                     /locus_tag="KPE55_003580"
     CDS             complement(451..2259)
                     /gene="dptF"
                     /locus_tag="KPE55_003580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HBG0494609.1"
                     /translation="MSNGCLIDELKKLKESSKEAVENISDFSSFKKYMHVKRQVENEL
                     LDIIKYTADSNKAELILVCGGVGDGKSHLISYLNNKYPEIMSKFIIHNDATESFEPNK
                     TSIDTLNDVLDDFSDKKMDNSDKKLVLAINLGALSNFLESEYVNKFTKLREFVENKGI
                     LDISISDDDDKNSNFHFVNFSDYHIYTLNADKPKSRYIESIINKIIAEDNQNIFINSY
                     KTNCTHCIVNYKCPIKNNYEMLMRDSVRETLVDILIESTVKYKIIISTRSLLNFIYDF
                     IVNVDLDNKNNKDLIKFVEELSYEAFIKYIFVSNLYNHSELSQILNAVNKIDPINITS
                     ENLDNIIIKLSNTKDMTILFNQYLNLSKNSYLESSMTNREIIESALNSSKNELSRKIL
                     KYNIINTFIRLHIFIPKNNLQLRDNIYISYMRNLYYFNKGEKTYLSNLYDDIKEALYS
                     WNGRIQDDRINMSIGKNQMRYKISQRLNIIPDLSKLRKCTEEELHKFKPSLEIGYKND
                     EKENQDTCTVDIDFNLYKLLMRVKYGYTPNNNDKYTYINFVVFMNKIHKLGNQNRELY
                     IEDRLEDTVPKYKLTYDKEFDKFKFIRVNGVKVCNL"
BASE COUNT          781 a          342 c          200 g         1070 t
ORIGIN      
        1 tatttatttt attttttata gttatattat ctgtactatt tagtaaaaca tcatataaaa
       61 atttagacaa ttttttataa taactatcag atggcatatg tatataattt aaaagctttg
      121 gatgaaatat atacatattt tcttcttcat ccaaaaataa ttctcttatt atactctcaa
      181 gttgtacact atcatcagaa gaattcacat ttatgtcttt tataagtaac tttatatctt
      241 ttagctgtct ttcttttatg ttcattttct tgttatatac caacctacag aattctccaa
      301 cacaagactc aaatctttct accttttctt taagtttcga tttttcttta gttgtagttg
      361 tatatgggag tataactatg tttttgacac tattatgtct cgcgatacct ttatctatca
      421 tataattttg tttaaagtat tctttattta ttataagttg cataccttca ccccatttac
      481 tcttataaat ttgaatttat caaattcttt atcatatgtt aatttatatt taggcacagt
      541 atcttctaat ctatcctcta tatatagttc tctgttttga tttcctaatt tatgtatctt
      601 attcataaat actacaaaat ttatgtaagt atatttatca ttattgtttg gtgtatatcc
      661 atattttact ctcattagta acttatataa attaaaatct atatccactg tacaagtatc
      721 ttgattttct ttttcatcat ttttataccc tatctctaaa ctaggcttaa acttgtgcaa
      781 ttcttcctca gtacattttc ttaatttgct taaatctgga attatattta gcctttgact
      841 tattttatat ctcatctgat tttttccaat ggacatattt attctgtcat cttgaatacg
      901 tccattccaa ctatacaaag cttctttaat atcatcatat aaattactta aataagtttt
      961 ttctcctttg ttaaaatagt ataaatttct catgtaactt atatatatat tatctcttaa
     1021 ctgtaaatta tttttaggta taaatatatg tagtcttata aatgtattta ttatattgta
     1081 ttttaatatt ttcctactca gttcgttctt tgaagaattc aaagccgact ctattatttc
     1141 tctatttgtc atagaacttt ctagataact gtttttactt aaattcaggt attgattaaa
     1201 tagtattgtc atatcttttg tattactaag cttaatgatt atattatcta ggttttctga
     1261 tgtgatattt atagggtcaa ttttatttac tgcatttaaa atctgagata attcactatg
     1321 attatataaa ttagatacaa atatatattt tatgaatgct tcataactta gttcttctac
     1381 aaacttaatt aagtctttat tatttttatt gtctaaatca acattaacaa taaaatcata
     1441 tataaaattc aaaagagacc ttgttgaaat tataatttta tatttaactg tagattctat
     1501 caatatatcc accaaggttt ctctcactga gtctctcatt aacatttcat aattattttt
     1561 aatcggacat ttataattga caatacaatg tgtacaattt gttttataac tatttataaa
     1621 tatattttga ttatcttctg ctattatttt attaattata gattctatat atctagattt
     1681 aggtttatct gcattcaaag tatatatatg ataatcacta aaattaacaa aatgaaaatt
     1741 cgaattttta tcatcatcat ctgatataga tatatcaagt attcctttat tttcaacaaa
     1801 ctctcttaat ttagtaaatt tattaacata ttctgattca agaaaattac taagtgcccc
     1861 taaatttatt gctaatacta actttttatc tgaattatcc attttcttat ctgaaaaatc
     1921 atcaagtaca tcattaagtg tatctattga tgttttattt ggttcaaaac tttctgtagc
     1981 atcattatga attataaatt ttgacataat ttctggatat ttgttattta aatatgatat
     2041 taaatgcgat tttccatctc ctactccacc acataccaat atcaactcag ctttattaga
     2101 atcagctgta tattttataa tatcaagtaa ttcgttttct acttgtcttt taacatgcat
     2161 atatttttta aaactactaa aatctgaaat attttctact gcttcctttg atgattcttt
     2221 taatttcttt aattcatcta tcaaacaacc atttgacata attttaaaac tccttctaat
     2281 atactctttt atactacact tatattataa atgctattta tttctttgag atccctttat
     2341 gtaaaaaaaa ccattatttt atataatagt aaattataat tatatataaa gta
//
LOCUS       DAELNY010000284         2355 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.287, whole genome shotgun sequence.
ACCESSION   DAELNY010000284 DAELNY010000000
VERSION     DAELNY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.287"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            213..1664
                     /locus_tag="KPE55_003581"
     CDS             213..1664
                     /locus_tag="KPE55_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0494610.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1883..2344)
                     /locus_tag="KPE55_003582"
     CDS             complement(1883..2344)
                     /locus_tag="KPE55_003582"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG0494611.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          813 a          270 c          402 g          870 t
ORIGIN      
        1 attaaatata tctatattat aaaaacaata aaaaaaggtt tgatttgaat ataaaactgc
       61 aatatattaa aaaaattatt cattttaaac cttgtaattt gattttataa atattataat
      121 taaattaatg aatttttaac aaggaattat tcattttttt gattttaaga tttattgaga
      181 aaacaaaact aaataggggg gtaattaata taatggattt tttcaatggt attttaacga
      241 gtattaacaa tgcagtagta tatgtaaaca attacttatg gtcgtatatc ttaattataa
      301 tgttagttgt aattggtatt tactttacta ttaaaacaaa ttttgtacaa ttcagatatt
      361 ttatagaaat gtttagactt ctgggtgatg gtacagcgaa taaaaatgca aaaaaggaag
      421 gaaaaatctc ttcatttcaa gcattttgta taagtacatc ttcaagagtt ggaactggaa
      481 atatagctgg aatagcaatt gcagtggtag caggaggacc tggtgctgta ttttggatgt
      541 ggcttatagc attaattggt tctgcatcaa gctttgttga gagtacatta gctcagattt
      601 acaaagtgaa agatggtcaa gcttttagag gtggaccagc gtattatatg gaacaaggtc
      661 taaataaaaa atggatgggt attttattct caatacttat aactatatgt tatggttttg
      721 tatttaatgc agttcaagca aatactgttt cacttgcatt taataatgca tttggaataa
      781 gtaaaatggc tatgggaata ggtcttggaa ttttaactgc aatagttatt tttggtggag
      841 tgcatagagt tgcaaaagtt tctgaaataa tagttcctgt atttgcagga ttatatatat
      901 tagttgcttt ggctatagtt gtaatgaata taacagagat accaagtgtt atagcattga
      961 tagttgaaag tgcatttgat tttaaaggaa tggcgattgg tacatttatg ggagttgtaa
     1021 tgactggagt gaagagaggt ctattttcta atgaagcagg tatgggtagt gctcctaatg
     1081 ctgctgcaac agcacatgta actcatcctg taaaacaagg tttaattcaa tctttaggtg
     1141 tttttacaga tactataata atatgtagtt gtacagcatt catggtatta ttatattcag
     1201 gttatagtag tagtggagca actggaattg aattagctca agaagcttta actcatcata
     1261 taggaccagt tggaaatata tttatagcag tttgtatatt cttgtttgca tttagttcta
     1321 tagttggtaa ttactattat ggtgaatcaa atatggagtt tatgagtgga agtaagacta
     1381 aacttaatgt atttagagtt tttgttgtag gtatggtttt atttggttca cttactcaag
     1441 tggatgtagt ttggaatctt gcagacttat ttatggcatt gatggcgata ataaacttaa
     1501 tagcaatagc acttttagga aaatatgcat ttattgcttt aaaagattat acaagtcaaa
     1561 aaaaatcagg aataaaagac cctgtgttta ttgcagatga aatagaaggc ctagaaaagg
     1621 tatcagagtg gcatagggaa ttatctgagg aacatttagg ataaaaaata gtataaaatt
     1681 ataaaaccat tttgaaagac tagagtatgt atatacttta tatgaatata atattgaatt
     1741 aatttatatt actggaattt ttaattccag taatattttt ttattatata atttagcttt
     1801 attaaaagtt tttattatta aaaatagctt atttatcgta aagtgaatga tattgggttt
     1861 taattattat gaatgttagt atttaattta aagacttaaa taaatttgcc atttctagcg
     1921 cattcatagc acaatcataa cctttatttc cagcttttgt acctgctctt tcaatagctt
     1981 gttcaatatt ttctgtagtt acgattccaa atataacagg taattcactg tctaaagata
     2041 ctttggctat ccctttagag acttcactac atacataatc ataatgagaa gtagcccctc
     2101 ttataacaca gcctaaacag attatagcat catacttacc acttttagcc attttttgag
     2161 caacaagagg aatctcaaaa gctccaggta cccaaactat ttctatattt tctggagaaa
     2221 cgtcatgtct taaaagacag tcttcagctc ctgataaaag ttttgatgtt ataaattcat
     2281 taaaacgaga atttattata cctattttta aatcttttcc aataagtttt ccttcatata
     2341 tcatttttac ttcct
//
LOCUS       DAELNY010000285         2282 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.288, whole genome shotgun sequence.
ACCESSION   DAELNY010000285 DAELNY010000000
VERSION     DAELNY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.288"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            158..1408
                     /locus_tag="KPE55_003583"
     CDS             158..1408
                     /locus_tag="KPE55_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0494612.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILVVGFITLLIAFVSKITLRNENL"
     gene            1569..2138
                     /locus_tag="KPE55_003584"
     CDS             1569..2138
                     /locus_tag="KPE55_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0494613.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT          809 a          307 c          355 g          811 t
ORIGIN      
        1 agagcaaacc cattccactc tactctaagc catctataat agtttgtata tttctaataa
       61 tattagatat taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta
      121 taaatatttt aataatctta gataggtagg tacatatatg gtaaaatcct cgaacacttt
      181 taataaaaac ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat
      241 acttaaattt gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc
      301 tactatacta gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat
      361 tgcggatata ttcaacagaa gaaacttaat ggttattttt gatttttcaa gtagtcttac
      421 agtactaatc ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat
      481 tattatgacc atattatcag tcataagcac aatgtatcaa ccaactgttc agtcaagtac
      541 acccctttta gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc
      601 atctcttaca aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat
      661 taatgcaatt atagttatca gttgtgtaag tttctttcta tctgcaatta tggagatatt
      721 tatacagatt ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac
      781 tgatataaag gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact
      841 ttttttagct atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta
      901 tatagtaaaa atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat
      961 ttcttttagt tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat
     1021 cactaatttg catatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc
     1081 agtatatcca tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc
     1141 atctatgcta atatttttta caacgactct tatctcaata tatgttataa cagtcacaca
     1201 attaaaaact ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc
     1261 aattgcaatg ccccttggtc aggttatgta tggtaaactt tttgatttat tatcagatag
     1321 agtgtacatc attatactag tagttggatt tattacatta cttatagcat ttgtatcaaa
     1381 gataacattg aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt
     1441 tcactaattt ctgaatttaa atgcaaaatt gtgtatcagt atgttgacgt tttagaaatt
     1501 gattgatata atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag
     1561 tgataaaaat gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca
     1621 ataaggaaga atttcatata agacatgctc aaattgtatc tggagttatg agatactatg
     1681 caaaggagta cgaccctgaa agagtagaat tttgggaaat agttggactt ttacatgatt
     1741 tagattttga aatgtatcct gatgaacatt gcgttaaaca aagagaatta atgacagagc
     1801 ttgatttaga taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt
     1861 cagatgttaa accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg
     1921 gtttaattgg tgctgctgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa
     1981 aatctgttaa gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta
     2041 taagaagtgg tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag
     2101 aagctatgag aagcttgatt ccagagatgg caatatagct cctccataca tataccatag
     2161 aagttacatc tttaaagagc atttctcttt ttgatgcaac ttctttttat attttgtaga
     2221 ataaaaagtt taatatttta aactcactat ataataaata atataatgta taatagtcat
     2281 aa
//
LOCUS       DAELNY010000286         2276 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.289, whole genome shotgun sequence.
ACCESSION   DAELNY010000286 DAELNY010000000
VERSION     DAELNY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.289"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..814
                     /locus_tag="KPE55_003585"
     CDS             23..814
                     /locus_tag="KPE55_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494614.1"
                     /translation="MTGCSPRESASEVAKKYIDKMNSGDYETAYSLLSKDSKKKISIE
                     NLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVVKLEETFNIKNLLYKKDDS
                     LKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLNDFGLKYFKEVDLILKKAIA
                     INPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSLKYLDKNDSDYKKNASNIY
                     NLKGVILMFHKRFDEAQQYLNKSLYFNKDNEDPKYNLNTIKKHLN"
     gene            complement(1038..1202)
                     /locus_tag="KPE55_003586"
     CDS             complement(1038..1202)
                     /locus_tag="KPE55_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494615.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(1744..2076)
                     /locus_tag="KPE55_003587"
     CDS             complement(1744..2076)
                     /locus_tag="KPE55_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG0494616.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          923 a          303 c          205 g          845 t
ORIGIN      
        1 aacattatta attgtgatat taatgacagg ctgttcccca agagaaagtg ctagtgaagt
       61 tgcaaaaaaa tatattgata agatgaactc tggtgactat gaaacagcat atagtttatt
      121 aagtaaagat agtaaaaaaa aaattagtat agaaaatctt gaagaatttc aaaatatttt
      181 agttagtaca aaaagaataa ttagttataa aataggtaaa gaaaaaatca taaaaaaata
      241 taaacatgat ggaaaagaat ataaaaatgt tgttaaatta gaagaaactt ttaatataaa
      301 aaaccttctt tataaaaaag atgattctct aaaagtaaat agatattttg tagttgaaaa
      361 taacaaatac aagctattat tgtatgaaag tttcaaaaaa gaatttggta ttaatagtat
      421 ggaccttgct caacttaagc ttaatgattt tggactaaaa tactttaaag aagttgacct
      481 tatactaaaa aaagctattg ctattaatcc tactgattca aagctttatt atgcccaagc
      541 atgtaattat atgaattttg atggatttcc tggttattca gatacaacat ataatgcttt
      601 agattcaatt aataaatctt taaagtattt agataaaaat gattctgact acaaaaaaaa
      661 tgcaagtaat atatataact taaaaggagt tattcttatg tttcacaaaa gatttgatga
      721 agcacaacaa tatttaaata agtccttata ctttaacaaa gataatgaag acccaaaata
      781 taatctaaat acaataaaaa aacatctaaa ctagatattt aatttttgaa tttataaaat
      841 atttttttat gtataattca gcactgaatc ataatctcta ctaaactaat tacaagttta
      901 aattataaag acttatttct atataaatat tactctataa caataagttt cttatttaaa
      961 ttttaaaata ttatctaaaa atcataaaaa agaaggtata aagagctacc ctcaagctca
     1021 atatacctta taaatatcta aacttcattt tttactaatt tttctatatc tttgataata
     1081 tgttctttag atttttttcc agctatggca tcaaaaattg caacagcctt tttctctcca
     1141 aacttttctg ctacatcatt aaagtctaaa aatctaacac tttcttttgg tttaccaaac
     1201 atataacacc tctcatacat ttttatatat ttattataac ttctatagac ttatttttca
     1261 ccaataaacg ttagacaaat tagtcccttt atttttttaa actagtttta ccttcaaaat
     1321 tcttaacaaa aacagtttat tctactctat aaataaaaat agacaattta atttgtagga
     1381 aactgataat agaatacagt aaataaaact ttgtttaatc tatctgaaaa agttatctat
     1441 taaaactgct atgaatttat aaagaatatt ctagttcata aaaatacaaa tatgtaaata
     1501 taatcacctt aatattcaat ttatttaaaa taaaaaatgt ataaaagatt taataatgac
     1561 aatatactat tctcaataca atctatgatg taaataattt aaaactacta tattatttaa
     1621 aaatttatat ttatatatgc aattctaaaa acttaataag aagtgaactt agaaaataaa
     1681 gcttaatttc aaagacttaa atagactatt attattagtc taaataaact acgtctatca
     1741 aaattatata ttaaattctt ctaaaatttt tattgcaaat cttgcgtttg gatatgtaat
     1801 agacccgcca ccaattacac ctatttttat agcttctata atttcatctt tacttgctcc
     1861 agaattaata caaccctcaa tatgataaca aatacactca tcacatcggc ttaaaattga
     1921 aatagctagt cccattaatt ccttattttt tttacttata gccccttctg aataagtctt
     1981 atcatcaaga cttccaaata ctctaaaaaa ctcatctgtt tcgcttaatt ttttattgta
     2041 aaatacccta tattctttca tttctctagt attcatcttc tacctcctat gtttttctta
     2101 taagaatatc acactgtact tatattttca atattaaata caaattttca ttttatgttc
     2161 attagcctaa tttatattat tttttgaaat ctcaataagt tatcatataa gtaagatttt
     2221 ttcagaacta aaatagaaat cttagtaatt tctttattac ctatctcagt tctgaa
//
LOCUS       DAELNY010000287         2268 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.290, whole genome shotgun sequence.
ACCESSION   DAELNY010000287 DAELNY010000000
VERSION     DAELNY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.290"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1472
                     /locus_tag="KPE55_003588"
     CDS             <1..1472
                     /locus_tag="KPE55_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0494617.1"
                     /translation="DENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIETLYIKIKEI
                     NDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLCNIKIGECE
                     CSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVAINNLAKSE
                     VYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILNMIKNIEEY
                     IIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKSIYLCFEKI
                     QNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVKLDLDKKKY
                     VDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDIALKNITII
                     IKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNLTVSIGIDT
                     CKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            1499..>2268
                     /locus_tag="KPE55_003589"
     CDS             1499..>2268
                     /locus_tag="KPE55_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0494618.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTE"
BASE COUNT          997 a          174 c          330 g          767 t
ORIGIN      
        1 gtgatgaaaa taattttcta attgaagaaa ttatttttaa atcacaactt atttacatta
       61 aggcaagtaa gtgtatagaa tcttctgtaa taattaaaga gatagaaacg ttgtatataa
      121 agataaaaga aattaacgat tttaattgta aaaaaatata tttttttatt attggtaaaa
      181 tatatttttt gtttttggat gatgctctaa ttgctaaggt aaatttttta aaagcaagaa
      241 aatatgcaga tttactaaag gatatgcaag tatcttcact atgtaatata aagataggtg
      301 agtgtgaatg ttcgtttgaa aattatgaat gttccatgga atatttcaat gaagtaataa
      361 gcaataaaaa gtatagaaat gtaaatataa ttcaaaaata tagagcatca aacaacataa
      421 ctaaaatatt aataaagact agaaattatc ctgtcgcaat aaataattta gcaaaatctg
      481 aagtatatct agaaaaaatt aaaaatcata gcttaaaaga agtagagaag tttgatttat
      541 ttataagttt agccatgtat tatgcaaaaa gtaatgaaaa atcttttgaa caatcaatat
      601 gctatttaga tagagctaaa aacatattaa atatgataaa aaatatagaa gaatatataa
      661 tatatgattt agaaataatt tatcaccagg caaagatata ttatatcttt ggatattatg
      721 ataatgcctt aaacacaaat aaaaaactcc ttgaaaaatc taaaaatgct aaaaattata
      781 attatgtgaa gttagcatac aagagtatat atttgtgttt tgaaaagatt caaaattatg
      841 aaatgagtat gaaatacttt aaaatgtatt atgagttaaa aatgatgtat ataaaagctc
      901 gaaatagaaa ttatatagat tccttaaatt atagacatga gtatatagaa aaagaaataa
      961 ataacatgag aaaagtaaaa cttgatttag ataaaaaaaa gtatgttgac catttgacaa
     1021 gtgcatataa tagaactttt ttgaacgatt ttttagaaaa acaagaagtt agtgaatatt
     1081 gcacagcatt tatgatagat gtagattact ttaaagaata caatgatact tatggacact
     1141 ataatggaga tattgctctt aaaaatataa ctataataat aaaaaaatat ctaaaaaaag
     1201 acatgcttat gataaggtat ggtggagaag aatttctgat attatctgta tgtaaagatt
     1261 ataaaaaaag taaaatattt ggaagaaaat tatgtaggat tgtaaagaaa tttttaaaca
     1321 ataatttgac agttagtatt ggcattgata catgtaaaaa tagtagtata gatataaaag
     1381 aaataattga aaatgctgat aaagctttat ataaagcaaa ggaaagtggt agaaatagat
     1441 gtttgcatta tcatgatttt aagaattttt aactgttgaa ataggattgg tgatttactt
     1501 gaaaaggagt ttaatagaag atttaaagag aaatataaac aagatagaat acgatatgtt
     1561 gactaaattc tatctaaatt tgacagattt aaactacatc ttggattatc acaaagacct
     1621 tttaaataca aaattagaag ataatagtag agtctatact ttatatttaa ttagtcttat
     1681 aaactatata aaaaaagata gagaagcatc atacaactat ttgatagagt cattaaagta
     1741 tattaaaaaa ttgtcattaa aagatgattc agatataata tctaaaattt atatttatct
     1801 tactttatca gctatttcta taagaaaata tgataaggta aataattttt atttctcagc
     1861 aaaaaaaatt ataagacaaa atcatttaaa tgagttgttg gtattgttaa atatgagttt
     1921 atgtgtagaa ataagtgctt tatatcagac caaaacggat gtaatagctt tagtagaaga
     1981 agtgtctttt tttaaaactt taaaaaataa agctcttgta agtagggcaa attatatatt
     2041 tgggagagtt tatctatatt tatttaataa ctttataaaa agtatggaat acttaattga
     2101 atgtctaaaa ttggctcaag aaaataattt atactctata gaggcttttt gtaaatgtat
     2161 aataagtttg tgttatttag agagtggaaa taatgtagaa gctataaaat acataaataa
     2221 tgtattagat gttgcacaca gtaataactc aatatcagta actgaaag
//
LOCUS       DAELNY010000288         2202 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.291, whole genome shotgun sequence.
ACCESSION   DAELNY010000288 DAELNY010000000
VERSION     DAELNY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.291"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            289..903
                     /locus_tag="KPE55_003590"
     CDS             289..903
                     /locus_tag="KPE55_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0494619.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            921..1283
                     /locus_tag="KPE55_003591"
     CDS             921..1283
                     /locus_tag="KPE55_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494620.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1389..1886
                     /locus_tag="KPE55_003592"
     CDS             1389..1886
                     /locus_tag="KPE55_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG0494621.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          845 a          243 c          344 g          770 t
ORIGIN      
        1 tctcatataa gtattactat ataataattt attctattag tatactcaaa tcatgtaaaa
       61 tttatataac tcaattaatt ttttaaatac aactttataa tataaaactt ataataaaag
      121 taaatttata tgttaaatga ggttgatttt ttattatttt ttatttatgc tagtactaaa
      181 gacttgcaaa cgttttttga caagtcttta ttttgtctaa attaaacaaa cttttttaca
      241 aataaataca taaaatttaa actaatctat ttattggagg agataaaatt ggaagaagta
      301 attgcaaaaa ttattactat agaaaatgga ttcaaagaaa ttgaaaacat tgctaaaaaa
      361 attgtaaaaa ataatgactc aaaaaagtgt tattatctat caatctactt atataggtca
      421 gaatattatc aagtaagaga attggctgtg tttatacttg gttttatatc agtaactatt
      481 tctgaagctt tgttattttt aaaagataat gtgagtaaag atgaaaactg gagagtacaa
      541 gaaatactag ctaaatcatt tgattattat tgcagtgaag taggatatga aaaggctctt
      601 cctgttataa aagaatggct taattctgat agtccaaaca taaaaagggc tgtgacagaa
      661 ggtcttcgaa tttggactag tagagactat tttaaatcta atcctgacgt tgctatatca
      721 ctactttcta gtttaaaaga agatgatagc gaatacttga gaaagtctgt aggtaatgct
      781 cttagagata taagtaaaaa atatccagat ttagtaaaaa aagaattaga ttcatgggat
      841 atatcaaata aaaaagttga gtttgtatat aagcttgctt caaaatgtat cttggcaaag
      901 taaaatggag gtgaacttat ttgaaacttc gtgatgagta tacttgccca ttagaactaa
      961 cacatgatat gataaaaggc aaatggaaat ctattatatt atggagatta cgcataggac
     1021 caacttcctt atcaaaactt gaaaaggaaa tcaatggtat taatcaaaag atgttacttg
     1081 aacaacttaa agaattaatt gaatttggtc ttgtagataa aaagacatat gaaggttacc
     1141 cacttaaagt cgaatactca cttacaccta ttcgagggaa acaaatattg gatgctttgg
     1201 ctataatgca agatatagga attagcttta tgttggataa tggtatggaa gatattctta
     1261 aggaaaaagg tgttttaaag taaatagata cccacaaaaa agtaagtaat ttaccattat
     1321 tttcaactgg tatataatta aattatagat atattctatt aatttattat aataggaggt
     1381 aaattattat gaaagtaact agtacaggta tagtaaatgg tgttatagaa gataaatacg
     1441 gtaaaagagg aactcagttc aatgaaggtg gtatgcctac ctattcacta cctctaaagt
     1501 ttgaagatgc tccaaagaat acagtatcct ttgctgtatt tttagaagat aaagactctg
     1561 ttcctgtatg tgggtttgca tggatacatt ggttagctgt aaatataaca aaagatgagt
     1621 taaatgaaaa tgaaagtgta acagctactg attttgtaca agggactact agttggcatg
     1681 gtaaactagg aggtcttgac agattagagg ccagtgtata tggtggaatg gctcctccag
     1741 acagacctca tgtctatgaa atacatgtat ttgcacttga tactatcctt gaccttgaaa
     1801 aaggattcta tatgaatgaa tttttcaaag ctattgaagg gcatgtttta gatacattta
     1861 cactaaaagg aagttattct aattaattag gttgactata gctaatcatt aattaaatgc
     1921 tacatttaat tgatgattag cttaattgtt attagaatat tttctgcact ttatcttaat
     1981 atatatttta gttgactggt attttaattt atattagatt attatcaatt tatataaatt
     2041 gattttagct taaaacttac ttatctattt cagtttctat tagaattatt attagactca
     2101 ttaaaataaa agcattaata taactaataa tatatgttaa attcattaac tctacataaa
     2161 gttttataat ctaaatgaaa tacaataatc tgcataaaat tc
//
LOCUS       DAELNY010000289         2200 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.292, whole genome shotgun sequence.
ACCESSION   DAELNY010000289 DAELNY010000000
VERSION     DAELNY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.292"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            24..476
                     /locus_tag="KPE55_003593"
     CDS             24..476
                     /locus_tag="KPE55_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0494622.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(811..2007)
                     /locus_tag="KPE55_003594"
     CDS             complement(811..2007)
                     /locus_tag="KPE55_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0494623.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT          789 a          319 c          294 g          798 t
ORIGIN      
        1 tttaaaacaa ccgaggtgat tatatgaact atatagtaaa agataccata agttttttag
       61 taaaaacatt tcctaagatt tattctagcc tgtacttaga agacctaaaa aaatttgcac
      121 cagattataa tgtaaataaa acacaattaa gagcattagt tttcataaaa aattatggag
      181 taataagtat gactgattta tgctctaagt taaatataga aaaaggcagt ttaactagta
      241 tggttgatga tttaactgat aagaagtatg taacaaggaa aagagattta gttgatagaa
      301 gaaaatatct aattgacatt actgaagagg gagataagat agcaactgat tttatggata
      361 aattaagtga tggtttagga gaaaaacttt ctaaattaac tgaagaagac agaaaaaaat
      421 atttagaagc aataaatact cttcaatata tattaaacaa agaagaattt agatagaaaa
      481 aatataaaaa tttttgtatt taatggtact aagattaatt ctttgttatt tatttaacta
      541 tataattata agtctaaata caatctaaaa agtacatctt aaattttctt gatgtacttt
      601 tttataattt ctctaactaa ggcaagacaa gttttagatg ctttaattaa agtgaataaa
      661 gattactggc caacactata taaagaaaat gaaaaatagt caggtctaag tcttaattaa
      721 tatattaatt aagaaatgtc cccttttcaa ataatatctg aaaaggggac attttatatc
      781 attaaattta tataaattaa gtttaaaaaa ttatttttgt aaagctacat attgctctaa
      841 accttctttt aataatgtag cagctctatg aaggtctttt tcatttaaga tgtaagctaa
      901 tcttatttca tcacgtccta aacctggagt agcatagaat ccttcagcag ggcaaaccat
      961 aactgtttca ttatctttat taaattcttt taacatccaa attgtgaaat cttctgcatt
     1021 ttcaactggt aatttagcaa ctatatagaa agcaccagtt ggttttttac atataacacc
     1081 ttcaaccttc attaattcat tgtataatac atctcttctt tttttgtact cttcattaac
     1141 ttctttgaag taagaaacag gagttttgta taactcaaca gctcctattt gctctaatgt
     1201 aggaacacaa agtcttcctt gacataattt aagtatttga gcgataagac ctttattttt
     1261 gcaagcgata gaaccaattc tagcaccaca agcactgtat ctcttagaaa cactgtctat
     1321 tattataact ctatcctgta caccctctaa gttaccacag cttgtatact caagcccatc
     1381 ataaacaaat tctctataaa cttcgtctgc tattatccat aaatcttttt ctttagctat
     1441 ttcagctagg atattaagtt cttcttttgt ataaattgca ccagttggat ttcctgggtt
     1501 agaaagaatt atagccttag ttttatcatc tactttagac aatatttctt ctttagatgg
     1561 aaggtggaaa ccgttttctg cctttgttgt aacagcatta acttctacgt ttacagattg
     1621 accaaatcca ttgtagtttg tatagaatgg ttctggaact aatagattat ctcctgggtc
     1681 acaaactgcc atcatagtga aaagtaaagc ttcactacca ccgttagtta ctaataattc
     1741 atctttttca aaattcatat cgtaagtttt atagtaattt tgaagtgctt ctaataattc
     1801 tggaatacct tctgatgtag catattctaa gacttcgcta tcaaagtttt taacagcatc
     1861 aaaaaatcct ttaggagttt ttatgtctgg ttgtcctatg tttaagtgat atactttgat
     1921 tcctttatct tttgctgctt gtgcaaatgg aacaagtttt ctgattggag aggcttgcat
     1981 tgcactaact ctgtttgaat aattcatttt aataccccct atttaattta aatacgtata
     2041 ttgtattatg taaattgtat gtttgaatac aagacatctt tacaagataa taataacata
     2101 cttatgggta aaaatcatca aaaaaaatta atatagcttt aaaataaact tttaacaaat
     2161 tatagtaaaa tacccatgtt tatgccacta aattatgtat
//
LOCUS       DAELNY010000290         2111 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.293, whole genome shotgun sequence.
ACCESSION   DAELNY010000290 DAELNY010000000
VERSION     DAELNY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.293"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(126..662)
                     /locus_tag="KPE55_003595"
     CDS             complement(126..662)
                     /locus_tag="KPE55_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG0494624.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(680..1993)
                     /locus_tag="KPE55_003596"
     CDS             complement(680..1993)
                     /locus_tag="KPE55_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG0494625.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
BASE COUNT          672 a          408 c          206 g          825 t
ORIGIN      
        1 gagtataatt ataaaaaaaa gaataggata tttttcctac tcttttttct ttacttatat
       61 ataatattaa aactctttat tttttaatat atatttttct tttaatatat ctcttttata
      121 tctctttaat tttcttcttc tatatcagag ccaaagggaa tccatgcatt accaaactct
      181 ttatctttaa ataaataagc taatccagtt atttgcttca atctaagaat ttcatcaact
      241 tccataccca ggtgctttga tatccattca tcagattttc caatttcaca aagttcagtt
      301 ataattttac tcattaaatc cacattatga gaacctcttg cacgattgtg tcgaatagta
      361 cttgccattc tttcatcaag agatttatca attactgaaa caggaagaag tccgttttct
      421 ctatcatata tatcctcatt ttcaagcatt actctatatc tatgaaaacc atctacaata
      481 atatacttat ccttttcctc attataataa cagacaattg gcattgtata cccatcttct
      541 tttatacttt tatacaacaa ctgcatttct ggtggggcta ctgcatttgg attatagtcg
      601 ttagattcta tcttttcgat tggcactgcg atgattccat atacaggact tttatattcc
      661 atagcatctt aactcctatc taatgaattt tttcatattt cttcttaata tattccgttc
      721 taattctctg ttcttttgta ggaccaaatc ccataaatct acacatatgg tcatttttta
      781 aaatacaata acacattctt ttccaacttg gtatatcttt ggtaaattta atatcatccg
      841 tatcatcagg tatatcatcc aaaaatatga ctctactctt tttatctttt gtatgatttg
      901 aaactccatt taattctatt ttataacctt ttctcaacaa ttcacgtata actatttctg
      961 acaaaccacc acctgtattc tgccaaaact caatagatgt tgaaaatttt ttaatatagc
     1021 tattccttat attttcaggt aaggtatcaa gcaaaaaata tgtgaaactt ttccatgtgt
     1081 gtccatatgg caatttaaca ctacgatatc ccatagcttt tgttttgcca taaatagaag
     1141 taaaattaac tccttgtact ctagcaacca atttcaccca tacatctggg tccaatattc
     1201 tgtataaatt taaactctct tttgctgaat cattaaaagg tgatgccact ctcatttttc
     1261 ttggagctac tccagcttta tagaataagt cataaatttt attatagtca tagtcaaatt
     1321 tatagtgtgc taaccatata tcttctattt cccaatcata tataggagat gccgtccaga
     1381 catttttaaa ttccttagtt atccagcact ttcctttata tccatgctgt ttattcacaa
     1441 tagaacaata acgttgtaga gattctgctg ctcgtgttcc taataagcaa acaacttttg
     1501 caccttcatg tatatctcta atccatctac caaattgttt tgccaaactt tcttgtctca
     1561 ttttatattt ataataagta aaaggattat ttttttgatt tattatgtat tctttgactg
     1621 gcatttccct tacccataac tcttctttct catcatccca tggataccaa tacatttcat
     1681 aatttcctac agcagttctt gtagccattg gtaaacacac ccaatatggt tcaatatctt
     1741 caatattatc ttcaaatact ttttcaacat attcagtagt caaactatat tgagcctcaa
     1801 aatcttgatg aaatactcct attttggaac ttatattatt ctttctctta taatccaaaa
     1861 ctaaatttag cagtaaacca ctatcttttc ctccgctaaa agatacatag atataatcaa
     1921 attcattaaa aagataagaa agtctttctt gaaaagcttc aaatacatcg gattctaaat
     1981 atctcttatc catttgaatt atcccctttc aattagaagt ttataaaacc ttacaatgca
     2041 atattattat gcttttttag cagtaatcag cataaattat acattattta aatacacaaa
     2101 tccaactaaa t
//
LOCUS       DAELNY010000291         2065 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.294, whole genome shotgun sequence.
ACCESSION   DAELNY010000291 DAELNY010000000
VERSION     DAELNY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.294"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            29..427
                     /locus_tag="KPE55_003597"
     CDS             29..427
                     /locus_tag="KPE55_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0494626.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFG
                     NIKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            415..1353
                     /locus_tag="KPE55_003598"
     CDS             415..1353
                     /locus_tag="KPE55_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0494627.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1737..1961)
                     /gene="secG"
                     /locus_tag="KPE55_003599"
     CDS             complement(1737..1961)
                     /gene="secG"
                     /locus_tag="KPE55_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG0494628.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          757 a          275 c          255 g          778 t
ORIGIN      
        1 aacttatata aaatcttaag gagttgccat gataaaaaaa atagaacatg tggtcattac
       61 taataataat atagaaaatt atatcaattt ttataaaaaa tttggttttt catctaaaga
      121 aatgagcaaa aattataaac ttaatgctag aaatgttaag ataaatctta acataaaagg
      181 ttataaattt ttaccttatg ttaaaaattc aaaagcaagt agagataact tatgttttaa
      241 aataaacaca aatattgatg agccaaaaaa atacttagaa caaaatgaga ttcaaattga
      301 gcgaggaatt gtagaatgta caggtgtctt tggtaatata aaatctattt atttgaaaaa
      361 aactaatgga aatttaattg aattatcaaa tcatagaatc acggagagta aaccttggaa
      421 aatttaatcc tatcaataaa tgttgttctt cctttgtttc tcacaatgtc acttggatat
      481 gttctaaaaa aattaggtct atttgatact catactttaa ataaaatgaa taatgtctgc
      541 ttcaagtctt tcttgccatt attattattt tttaatatat acaatacaga tttagatggt
      601 tctcttaatt ttaagcttat ggctgtagct gttataatta ttttttcaat cttcttaatc
      661 ttagtcttta ttattcctaa aattgaaaaa gataataaga caaggggtgc attaatacaa
      721 ggtgtatttc gtagcaattt cgttatattt ggtattcctg tgacaatttc actatttggc
      781 gataagagta ttggagtcac atctttatta attgcagtta ttgtacctat gtttaatgta
      841 ttatctgttg taattcttga aatttttaga ggtggcaaag taaatataaa acatatgatt
      901 aaaagcgtct taacaaatcc actcataatc gcctctttta taggattgac aatgctaatt
      961 ttaaatatta atatacctac atttttacac aaaactattg gtgatatttc taaaatagct
     1021 actcctttat cacttataat tctaggcggc tcttttagtt tttctgctat aaaggggcat
     1081 ataaacccca ttatattggg cgtatgtgga aaacttatac ttgtacctgc tatattttta
     1141 ccaattagca tttttcttgg atttagaaat gtagaattag cttcacttat gataatgtta
     1201 tctgctccaa ctgcagtttc ttcatttaca atggcacaac agatggattc tgacagtgaa
     1261 ttagctggac aaattgtagt atttacttct ggattttcta taattacagt atttttaata
     1321 atatttattt taaagcagaa taatcttatt taatgatatt tgataacaca aattggtatt
     1381 atgagaaaca tatataaaat tgatttaact gcgtaaaaac atattattat actattttta
     1441 ctatataata taactttgtg tatattatct cattaaacat ggtgaaaata gtgttgctat
     1501 atatttttta atttgaaaaa aatatataaa tacaaacaat atataaatat aaataataaa
     1561 cgccaacaaa tagtcaatct ataaaataaa aactggacta atcatataaa atcattagtc
     1621 cagcttttat tttacgtact ttttatgttg atgtactttt attaaaaact aaatttaatt
     1681 tttaaatgtc tagcaaatgc actaattaaa taatggccag ttattcaaca atatcactac
     1741 ttcttaacta taagtaatgc caatgcattt ataaaaaata atactgctgt tatctttgta
     1801 actctagcaa gaaatgcttg ttttcctttt ggtttaaagt aagcttggct tccttctcca
     1861 ccaaattctg aaggtaaagc attcttagaa tcttgaggca ttatgctaat tactagtaaa
     1921 atccctagta ctacttgcac acccattaaa atgttgctca ttcttgcccc tcctatctgt
     1981 ttattactta aatttcatta ctctttagtg tatcacaata atatagccat ttcaagacta
     2041 ttatgctata aataacatac aaaaa
//
LOCUS       DAELNY010000292         2022 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.295, whole genome shotgun sequence.
ACCESSION   DAELNY010000292 DAELNY010000000
VERSION     DAELNY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.295"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            298..582
                     /locus_tag="KPE55_003600"
     CDS             298..582
                     /locus_tag="KPE55_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494629.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(739..912)
                     /locus_tag="KPE55_003601"
     CDS             complement(739..912)
                     /locus_tag="KPE55_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBG0494630.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1353..1568)
                     /locus_tag="KPE55_003602"
     CDS             complement(1353..1568)
                     /locus_tag="KPE55_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0494631.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1579..>2022)
                     /locus_tag="KPE55_003603"
     CDS             complement(1579..>2022)
                     /locus_tag="KPE55_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0494632.1"
                     /translation="ILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKVIALIARIL
                     YFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECINGYNLNTNNI
                     RIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          808 a          217 c          247 g          750 t
ORIGIN      
        1 tacagtttct ttatttttta ttattgtact tggcgtttta tattaactat atggaatgta
       61 aatacaatac ccccctattg caatgcttca ttaactatta gttttatatt aactatatgt
      121 aatataaatg ttttttctta ttaaataaag attgagtcac tttatattta ataatacata
      181 ttctaaattg attttgtttt tatataagag aatttgaata atatggaaaa agatgattaa
      241 taaattatta attagggtat aaaatgatat aaatagaata aataaggggt ggatgaaatg
      301 cttgtatata ataaaagttt ttatcctaat gacatatttc caagattaga tttttcaaaa
      361 ataaaaaaac agttaaaatt gatagataat gacctgtcag attttggaag catatgtata
      421 atagaaaaag aacattatac aataagtgta aacagtatag gtgaaataaa tgtgtattat
      481 gatttagagt acgaaaataa ggtgtataga atagtttatg agattgaaaa gttatttaaa
      541 tctcaagttg gaaggtttag tatatctaca tacagaaatt gataattaaa aaagtagaaa
      601 ttaaaaaact taatactaaa gatatagata aatatatgag gtaacagcta gagagagttg
      661 ttaccctttt taaatactaa gtatatatta ttcttattaa ttttttgtaa attcactaaa
      721 agaccttttt atagaatatt attttctttt tttattaaat aggtaaatca gtaaaaatgc
      781 aattccagtt ccaattatta ttccaatgat attttctata attaattttt ttaatataaa
      841 agtaaataat aaaaatgcta tacataaacc taaaatatat cctttaaaat aggttgaaag
      901 ttttcttttc atataatcac caacttgtat cagatttagt ttacttaatt agagtataac
      961 agtgttgtaa cttcaaagag tatctaaaag attacaaatg ttagataaaa tcatcaataa
     1021 tgtaatttat aacacgtagc tataaactat gcaataatat aaatggaggt ataagtaaat
     1081 gaaaaagttc catgttttgc taacagttta aacaaatatt aggtagaaaa gaatgtatat
     1141 aggaatctgt ggtaaaatta tcaatttaaa agtatgattt agtgtgatac agttcttata
     1201 ggtaatggat aacaaataga taaaggaaca gtttctttct tagaacatat tttgttatga
     1261 ctttaaacaa agttaatatt aatgtcaata atatagtaaa agaataaaaa tcatatagta
     1321 taataaaaaa gagtgtttct aaacactctt ttttaatcat tttcatcttc tacatattca
     1381 agtatatcac ctggctgaca gtctaaagct ttgcaaattt cgtttagagt ggtaaatcta
     1441 atagcttttg cttttccagt ttttaaaatt gataaatttg catttgtaat atctattttt
     1501 tctgccaaat ctttaagtga tattttcctt tttgccatca ttacatctag gttaactact
     1561 attgccataa catcacctct atattgttaa atcattatct tcttttattt ttatagcttt
     1621 atcaaatgtt tctgcaatta caaagcacat tagtgcaaat ataaaagaga taaccataag
     1681 tggtgagata ccgccactat cagttccaaa aattcttata ttattagtat ttaaattata
     1741 accatttata cattcaatta tagtgtttat caataaacaa catcccatta ccttaaacct
     1801 ttttacattt tcaagtacga atggggaata cttagtacta ttaattataa atatcaagaa
     1861 taagatgact ataaagtagt cagaaaaata tagtattctt gcaattaaag cgattacttt
     1921 gtcagaacta aaatcatata aaaacatagg tatcatacat aagaatatta taccagcaac
     1981 tcctaaaagt gataatagaa aatatagaaa tactgataat at
//
LOCUS       DAELNY010000293         2004 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.296, whole genome shotgun sequence.
ACCESSION   DAELNY010000293 DAELNY010000000
VERSION     DAELNY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.296"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            227..601
                     /locus_tag="KPE55_003604"
     CDS             227..601
                     /locus_tag="KPE55_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0494633.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            598..1461
                     /locus_tag="KPE55_003605"
     CDS             598..1461
                     /locus_tag="KPE55_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494634.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1461..>2004
                     /locus_tag="KPE55_003606"
     CDS             1461..>2004
                     /locus_tag="KPE55_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0494635.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIIL"
BASE COUNT          766 a          204 c          305 g          729 t
ORIGIN      
        1 tttttttaat ttagtatttc caatttattg gcaggatttt tgaacaatat ttataaattt
       61 tttaaaaaaa tttatatcta tttaatatgg cagactttca taaaatacat tacatttatt
      121 aaatgtataa tattggcaat tagttttttc taaaatcagt tgacatatta tatatatatt
      181 tatataatgt gtatatacta tatatacaat ttaaggaggt gtcttattga atatagttat
      241 tagtaattcg agtgggaagc caatatatga acagattact acacaaatta aaagtatgat
      301 tataagtgga gagttaccag aaggctcagc tcttccaagt atgagactat tggcaaaaga
      361 acttagaata agtgtaatta ctacaaaacg agcatatagc gatttagaac gagacggatt
      421 tattgagaca gttactggta aaggcagttt tgtatctagt aaaaacatgg attttgtcaa
      481 agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa gcagttgata cagcaaaaag
      541 tagtgatatt acatatgacg aattaaaaga tattttattt ttattatata aaggagaatg
      601 ataagtatgg atgcaataac tataaaaaat ttaaataaaa cttataaaga ttttagccta
      661 caagatatat ctttttcagt accaaaaggg agtgtaatgg gatttgtagg tgaaaatggt
      721 gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa tatcttatga tagtggaaat
      781 atagaaatct ttggtcttga tagtaaaaaa aatgaaaaag aaattaaaga gcaaattggg
      841 gttgtatttg agggaagcaa ctttcatgaa aatttaaaca ctgaccatgt ttcaaaaatc
      901 atgagtaaaa tctataaaaa ttggaatgac acattattta aagactactt aaaaaaattg
      961 agagtaccag acaataaatt aattaaggag ttttcaaagg ggaataagat gaaacttagt
     1021 attgctgtag ccttatctca taaaccaaaa ttattaatac tagatgaggc aactagttca
     1081 cttgacccta tcgttcgtga agaaatacta gatatatttt tagattttat acaagatgaa
     1141 gaacactcta ttatcttatc ttcacatatt acaagtgatt tagataagat agcagactat
     1201 attacattta tacataaagg aaaaattgta tttagtgaaa ataaggatga attaattgat
     1261 actatgggag ttttaaaatg taaacctagt gattttgata acctttcaag agaggattat
     1321 tcatattatc gtaaaagcca atttggatat gaagtacttc ttaaagataa gcatagattt
     1381 atctcaagac atccaaactg tattgttgat aatacgtcta tagaagaaat tatgttattt
     1441 tatgtgagag gtgataaata atgaaaggtc taattttaaa agatttactt aatttgaaag
     1501 gaaatataaa gtttatactt ttattcatca ttatgtttgg ctttatgtct tctttaggag
     1561 atggaaatgt taataacttt ataggtgtta ttatagtttt atgtactaca atgattgttt
     1621 caacattttc atatgatgat ttgaataaat gggatagtta tgtacttaca atgcctatta
     1681 atcgcaatga tattgtatta agtaaatatt taactatgtt aatatttagc tttataggtg
     1741 ttcttgtttc acttattgtt agtgtaacta ttggttattt taaaaataca cttatattaa
     1801 atgaaacact acttattaat gcactaatct tatcaatttc agtatgtttt ggtagcctta
     1861 ttctaccact tatttataaa tttggtacag aaagagctcg tttattaatg attttatgct
     1921 ttttagttcc aacactagct ttattagttt ttaaaagtat tttagaaaat attagttcac
     1981 ctatttctat agaaattata ctta
//
LOCUS       DAELNY010000294         1976 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.297, whole genome shotgun sequence.
ACCESSION   DAELNY010000294 DAELNY010000000
VERSION     DAELNY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.297"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..694
                     /gene="cprR"
                     /locus_tag="KPE55_003607"
     CDS             35..694
                     /gene="cprR"
                     /locus_tag="KPE55_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG0494636.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            905..1963
                     /locus_tag="KPE55_003608"
     CDS             905..1963
                     /locus_tag="KPE55_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0494637.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT          759 a          171 c          333 g          713 t
ORIGIN      
        1 gaatgctatt agatggataa atggaggagt tattatgtct aagatattag tagtagatga
       61 tgagttagat atgttaaagt tgattgaaaa tgtgttgaaa agagatggac atgaagtaaa
      121 ggtaattagt gatgtagaaa aggctatgaa catggatttt aattatttta atctaataat
      181 attagatgtt atgatgccta agatggatgg ttttgaagta tgtaaaacta taagaaataa
      241 ggttgattgt ccaatactgt ttttgacagc aaaaaatatg gaaagtgata ttatgtacgg
      301 tcttggtata ggtgcagatg actatataac caaacctttt ggtgtaggag aattaagggc
      361 taggataact gctcatttaa ggcgagaaag tagagaaaaa aagaatttat taactatctc
      421 aaatgttcaa tttaactttt taggcaaaga agtttcaatt gatggtaaaa aaattaattt
      481 tactaaaagt gaatatctaa tatgtgaatt tctagcaaaa aataagggac aagtattttc
      541 aaaagaaaga atatatgagt cggtatatgg ttttgatgga gaaagtgata taagtactat
      601 cacagagcat ataaaaaata tacgaagcaa attaaaacaa tatgatattt atacaataga
      661 aactgtatgg gggatagggt ataaatgggt gtaataagta taagtgttgt ttagtgaaaa
      721 tattatatat atctacaaaa tgtttgtaaa atattaatct atatattaaa atttaattta
      781 tagattaata ttttttgtaa atgacaaaat tgtaaggtat tttgttataa aatattaaga
      841 ttcatagtgt atactaataa tatagtattt agtataaatc ttgagcaaag gatggttatt
      901 tctaatggat agactaaaga atattagaaa gagtttacta cagtatacat ttttattttt
      961 gttaataggt ataacaattt acctagtgtt taaaacattg gatattaaaa tgctatcaaa
     1021 tgttatggtt atggtagata agaagttttt attcataggt gctttggcaa taatgtgtca
     1081 cataatgctt gaaggagtag taatgaagat aattatagaa agcactcaca aagttaatat
     1141 tagatttatt ggttttaagc tagccattat gggattttat tataatttaa taactccttt
     1201 tgcttcagga agccaaccag tacagatata tgtacttaaa aaatgtaaaa tgccacttag
     1261 taaggcaagt gcagttgtta ccaataagtc tattatcttc caaatagtag ttacttttta
     1321 ttgtacaata cttgttttaa tgaattttgc tatgttagaa aaacaaatga aaatgattat
     1381 gcctctagtg tttctaggga tagccattaa ttcatttgtc ttaattatga taatattagt
     1441 tattttaaat cctaaaaaaa taaaatattt tatgagaatt actataaagc atttatcaaa
     1501 aattaagttt ttaaaatttt tagataataa gattgaatcc atagaaagtt ttatagatga
     1561 ttatagtaaa gcaataaatt tttttgcaaa gaataaaaag gtattaattt caactataat
     1621 agtaactttt attcaattaa gtgcttactt tagtgtttca ttttggatat ataaagcatt
     1681 taatttacaa ggttatagct atatttatat actgacatta caagcatttt tatatatggc
     1741 aatatctcca ataccaacac ctgggaatgt aggagctaat gaattagcct ttttcactat
     1801 attcaaatca gtattccctc aacctttgat gggatatgca gtatttttat atggtggatt
     1861 tatgtactat ttgattctga ttggaagtgg aatatttact gtaattactc actatagaat
     1921 gaaaaataga attggcagaa atattgtttt aagtgaaaac taaataagtt taaaga
//
LOCUS       DAELNY010000295         1946 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.298, whole genome shotgun sequence.
ACCESSION   DAELNY010000295 DAELNY010000000
VERSION     DAELNY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.298"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            426..1766
                     /locus_tag="KPE55_003609"
     CDS             426..1766
                     /locus_tag="KPE55_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0494638.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          780 a          142 c          299 g          725 t
ORIGIN      
        1 aaatttccat ttgtagtgtt taaatataaa tctataacag catttctata aatgttataa
       61 atcactataa tgattcaact caaagttact gcttaaagaa gcagagattt tatcaaggta
      121 tatcatggaa aaaaatattt tattgtaata tttataagat tttattatgg aaataatgta
      181 aaaactttat aaataatata ttttatacct agttatagat aaatttatat agttattatt
      241 aaaaaaagca ataaaaagtt taattcaatt tagcaaattt agtgatataa ttaaaaaaga
      301 aaagaaatat ggacttatta ggaataattt tgacaatcta tttactacac gtgtagagtc
      361 gtatataatt aaattataca taaaatataa aaaaataatt tagtaatacg ttaggagaaa
      421 gtattatgat aaagtcaagc ttattttgga atattttcat tgcgatatcc tttatgatag
      481 aatggtttct ttgtaagaaa atgctagatt atacaagtga gaaaaggatt tctaacaaaa
      541 aaataaactt ttgtatggta ggtattattg tttttgctga cataatatat ttttttgatg
      601 tacctccaaa tataagagta attatatcta tgataattac tattgcattt tataaaatct
      661 catatgatgt ggggtttata aagtctgtat taatatcctt aatttattgg atgttactaa
      721 tcgccacaga tgctttgagt atatctttaa cagtttggct taattctata aatgacatga
      781 gtagtatggg gacgggagat atatacagaa ttgagtctat aatattggga aagatacttt
      841 tgattgtgtc tttgctttta tatagagcct taaagataaa acttaatata gaaataaata
      901 aaaggacatt attttatata gttattccta tagtcgctaa tatagcatct ttttttgtaa
      961 tttttgaata catatttaat ccagggtctg gtagcttaat acataatttc caattcgtta
     1021 tgatttctgt tgtattgctt atatcaagta tcactttagt tttaattgct aaaaaattac
     1081 agcaatataa tactttagtt gtagaaaaca atatgattaa aaatactttg aagtatgaaa
     1141 atgagtatta tgaagatata gaaaagaaat atttaaaaac tagattacta agtcatgata
     1201 tgaaaaatca catattgtgt ataaatgcta tgattaaaaa aggtattgat gttacttctt
     1261 atttaaataa tttgcaggta gaaattcaga gtaatgactt gttatttaat actggtaatt
     1321 ttatattaga tgctatatta agtgaaaaga aagaggtttg tgataagaat gatatagaac
     1381 taaaaatagg aataaatttt agttgttgtg aagctgttga attagtggat atatgtcata
     1441 tattttcaaa tattttagac aatgcaattg aggcatgtaa taaaattgat agtggaaata
     1501 gagttatttg tgttaaaggt gatgttatgc aaaattttta tttaataaga gttgaaaata
     1561 ctaagatgaa taagattatt actaaaaatg gagatatttt gacagacaaa aaaggttctc
     1621 ttcatggact tggaataaaa agtgtaaaaa actctgtaaa taagtatggt ggtaatgttg
     1681 taattgaata tgatgataaa aaatttgtag ttaaaattgc tattccactt agaaatgtga
     1741 agaaagcagt taatcatgtg atttaaatgt atataaattt gatgtgtaga aatttaatat
     1801 ataagaatta atgtataaaa gtgatgcata gaagtttaat atataagaat taatgtataa
     1861 aagtgatgta tagaagttta atatataaga attaatgtat aaaagtgatg tatagaagtt
     1921 taatatataa gaatttaata tgtata
//
LOCUS       DAELNY010000296         1939 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.299, whole genome shotgun sequence.
ACCESSION   DAELNY010000296 DAELNY010000000
VERSION     DAELNY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.299"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            141..980
                     /locus_tag="KPE55_003610"
     CDS             141..980
                     /locus_tag="KPE55_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494639.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            970..1413
                     /locus_tag="KPE55_003611"
     CDS             970..1413
                     /locus_tag="KPE55_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494640.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            1424..1915
                     /locus_tag="KPE55_003612"
     CDS             1424..1915
                     /locus_tag="KPE55_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494641.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
BASE COUNT          777 a          137 c          331 g          694 t
ORIGIN      
        1 ttctaattat tttaaattta agatacaatg attagaagga attttttaag tttcttagaa
       61 tactgtaaat tatataatct tgataaaaag tgttatacta tatataatct aaataaaaaa
      121 tagtacaaaa aggagtattt atgtttggat atgttaaaat aaacaaaatg gatttaacat
      181 ttagagaata tgattattac aaagcttact attgtggact atgtaaatac ttaaaaagaa
      241 atcatggaga aatctctagg ttttctttga actatgatat aacattttta attgttttat
      301 taacagcagt atataaccca gagtctataa gtacagagga agtgtgtata gtaaatccat
      361 ttaaaaagaa aaaggtaatt acaaatgata ttacagaata tgcagcaagt atgaatattc
      421 ttttaacgta ttataaattg gaagataatt taatggatga taaacgcata aaagataaat
      481 tggcatatta tatatataaa aacaaattaa aattagcata tgaaaagtat cctgaaaaag
      541 cagagtatat aaaacaacaa ctaaatgaat taaataagtt agaaaaagat aaaaatataa
      601 atatagatga agtatctagt atatttggaa atataatggg tgaagtattt gtctataaaa
      661 aagatgaaaa tgagagaaac ttaagaatga taggttttaa tataggtaag tatatatatc
      721 tactagatgc ctatgaagat ttagatgaag attttaagaa aggaagatat aatccattta
      781 tagagtatat tgataaaaat gatgagttaa aagaaaaagt aaaaaaaata atagtaacat
      841 cattaggatt tttagctagg ggaattgata atctaaatct aaataaaaat gttggaataa
      901 tagaaaatat aatttattct ggtgtttatt taagatatat taatatatta gaaagtagag
      961 gtggaaagaa tgtacagtaa tacttataat aatgaaaatt ttatgagagc aagatcattc
     1021 atagaaaata gtgaatttaa aaaagcatat gattttttaa aaactttgac tgacaaatgt
     1081 gcagagtggt attatttaac tggtttttct gcaatgaaca taggatatta tgaagaaggt
     1141 gaagacttct taaaaagagc aaaatttatg gagcctgaaa atagtgaata tagtgatgca
     1201 cttagaagtt atacacaata tagaaatgat tatagcaata gggctgacaa ttataataga
     1261 agaagacgta atgatttaga tggatgttgt tgctgttgct gtgatgattg ctgttgttgt
     1321 ctaggtgacg attgttgtga aaattgtgct aaactttggt gtttagattc atgttgtgaa
     1381 tgttttggtg gagatttaat aacttgttgt taggatgata aatatgagta taaaagttgc
     1441 ttatggagga gttttattag cattaaatgt gatattgttg actcttacaa acattattcc
     1501 tgtaaataca ttgtttatta tggggttagc atcactttta gtatcaattg taataatgga
     1561 atggggcttt aagagtggaa ttgcttttta tataggttct atagttttag gatttatagt
     1621 gatggcaagt aagtctcaat ggattgtata ttcacttacc tttggaatat atggtattgt
     1681 aaaatatctt atagaaaaag ataggtctat atatattgag tattttatga aattagtatt
     1741 tgcaaatata atgatactta tattgtattt tttattaaga acaatagttt atattccaat
     1801 aaacatattt attataggtt catttgagat agcctttata gtatatgact atgtttacag
     1861 ttcttttata ggttattaca ataatagatt aagaaagatg ctatttaaaa aataatagtt
     1921 gatttttata tttaatttt
//
LOCUS       DAELNY010000297         1926 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.300, whole genome shotgun sequence.
ACCESSION   DAELNY010000297 DAELNY010000000
VERSION     DAELNY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.300"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="KPE55_003613"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="KPE55_003613"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="KPE55_003614"
     tRNA            242..316
                     /locus_tag="KPE55_003614"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="KPE55_003615"
     tRNA            321..395
                     /locus_tag="KPE55_003615"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="KPE55_003616"
     tRNA            401..476
                     /locus_tag="KPE55_003616"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="KPE55_003617"
     tRNA            482..558
                     /locus_tag="KPE55_003617"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="KPE55_003618"
     tRNA            569..643
                     /locus_tag="KPE55_003618"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="KPE55_003619"
     tRNA            658..742
                     /locus_tag="KPE55_003619"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="KPE55_003620"
     tRNA            752..825
                     /locus_tag="KPE55_003620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="KPE55_003621"
     tRNA            836..912
                     /locus_tag="KPE55_003621"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="KPE55_003622"
     tRNA            922..997
                     /locus_tag="KPE55_003622"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="KPE55_003623"
     tRNA            1009..1084
                     /locus_tag="KPE55_003623"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="KPE55_003624"
     tRNA            1087..1175
                     /locus_tag="KPE55_003624"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="KPE55_003625"
     tRNA            1193..1283
                     /locus_tag="KPE55_003625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="KPE55_003626"
     tRNA            1292..1368
                     /locus_tag="KPE55_003626"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="KPE55_003627"
     tRNA            1454..1529
                     /locus_tag="KPE55_003627"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="KPE55_003628"
     tRNA            1590..1666
                     /locus_tag="KPE55_003628"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="KPE55_003629"
     tRNA            1673..1749
                     /locus_tag="KPE55_003629"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="KPE55_003630"
     tRNA            1753..1828
                     /locus_tag="KPE55_003630"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="KPE55_003631"
     tRNA            1836..1912
                     /locus_tag="KPE55_003631"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          486 a          442 c          523 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcactgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatcaa
//
LOCUS       DAELNY010000298         1919 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.301, whole genome shotgun sequence.
ACCESSION   DAELNY010000298 DAELNY010000000
VERSION     DAELNY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.301"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(145..306)
                     /locus_tag="KPE55_003632"
     CDS             complement(145..306)
                     /locus_tag="KPE55_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494642.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(648..1808)
                     /locus_tag="KPE55_003633"
     CDS             complement(648..1808)
                     /locus_tag="KPE55_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0494643.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
BASE COUNT          699 a          349 c          189 g          682 t
ORIGIN      
        1 atttactttt cacttaagac cattatttat cttataaaat tgagtactta taaactttat
       61 cacaaaaaaa cctaacttaa gtatttatat tctacataag ttaggtttta taaactatta
      121 taatatttat tatttcattc atttctaatg atgattctcc ttttttatct ttttcaattt
      181 gccttcacta acaacaaata aaaatcccat cttcttgtat atttcatcca attcaattaa
      241 agttacttca ccatttttta aatttgttaa tgttatatct tgtattcttc ttgatggaat
      301 tatcataagt tatcaacact cctttaaatt ctttttaatc tttatattta aatatattta
      361 tgcattctga tatatattca taaattatta aaataaattc aaaaaacacc cgttctacaa
      421 acattcgttc tgtatattta ttatacatca tacagacgtt cgattcaata taaaaattaa
      481 atatatctcg tttttagtaa attttacaat attatacact aaatatgtca aaacatcttc
      541 ttgaaataga ttatcttgtc aaacaaaaaa gccatttcag taaaagcttc tgcttaaatt
      601 gaaatgactt ttcttattta aattattaat tttataccat atttttatta agctaacata
      661 tgtaatattt gagttaccat aagagcacaa taagtcccta taatatttcc tagaagcccc
      721 ataagtacac ctactggtat taatacagga ttgtgaaccc cagcaatcat tggagctgta
      781 gttactcctc ctatattagc tatacttcct acttcacaag taaataaatc aagtttaaat
      841 agtttagcaa gccctataaa tgaaatagca tgtacaagga ctactaccaa tccaaacatt
      901 atgtaaacag gtgcttctga caatccaaag aaatttgctt tactacctgt agctgcaagg
      961 agtaaataca taaacacatt tcctaccagt gtactccctt taagctttga tatttttgtc
     1021 atacccaata atattgatac tgtagatgca acaagtacta cccacatagt ttcattaagt
     1081 atacctgaag ttggaagtat cttggataca atttgtgcaa aagacgcaac tccaaaccca
     1141 acagaaagta aaatcataag ttctacaaac gtaggtgccg tattatcttt attttctttt
     1201 tcaagttttg atgtaacctc atctataaaa cttgtatcgc actttgtcca tttgttaaat
     1261 ttaggtccaa atttcacaga cattagacaa acagacatcc atactgtata tcctatagaa
     1321 cccataagta ttgcataact aagtccttct tctgcaactc ctagagcatt agctgctgca
     1381 atcatatttg atgagccacc tatccatgaa ccagatgctg ccccaagaat tttccatgaa
     1441 tctgcaccca ttgaattttt aaaaattatg aatgctataa caaatccaag cataaatgta
     1501 aatgtagcac agaaaaatga taataacatt ttaggcccca tctttaatat ttttcttaaa
     1561 tcacattgaa ttaacaaaaa cactaacatt aatggaagga agttcactgt tattatgttt
     1621 tgatacgttc caacatcttc attctgtgca aataaaccaa atgatccaag tatagctgaa
     1681 cctaagtata ttaaaagcat tggattaata acatcaaata ccttccattt caatttatca
     1741 accataaaaa aaattacacc tacgaataca accaagaaac tacaatatac aaatccagtc
     1801 tcaatcacat tatacacacc ctttcttttc ctaattaatt tgatttttat gatttctaag
     1861 taaagacttt ttcagataat agacaattaa aataaatact tttatctata tcatactaa
//
LOCUS       DAELNY010000299         1913 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.302, whole genome shotgun sequence.
ACCESSION   DAELNY010000299 DAELNY010000000
VERSION     DAELNY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.302"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            355..1683
                     /locus_tag="KPE55_003634"
     CDS             355..1683
                     /locus_tag="KPE55_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0494644.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          671 a          211 c          338 g          693 t
ORIGIN      
        1 agatagaaaa ctacagaaag aagccaaaat gtttttgaaa aaaatgaaat tttattgcaa
       61 aaaaatcaaa atgaatttaa cttaatagtt ataatctatt tttagtgtta taaatataca
      121 atgtatatta tacaatataa aaaatacatt tacttagaag tattattata tttggatttt
      181 tggtttttat aattttttta tatttaaaca taactaaaaa taaaagatta ctaaagctaa
      241 gtagagttta ttttatttta ataaataata aagctatttt tctatatatt ggcataatat
      301 atgcttattt aactatgaaa ttaaaattaa ataggattaa tgaggaggaa aaaaatggga
      361 agtaatgaac taaaaaagac tcttggcgta tcagcagcat tatcaactgt tgttggttcc
      421 gttataggag cgggcgtatt ttttaaacca caagcagtct atacattaac tggaggagct
      481 ccaggtcttg gtatattggc atggttaata gctggaatca taactataac agctggtctt
      541 acagctgcag aggtatcagt tgcgatacca aaaactggtg gaatgatggt atatataaaa
      601 gaaatttatg gagaaaaatt aggtttttta actggatgga tgcaaattgt attattttat
      661 ccagggatga tggcagcatt aggtgttata tttggagaac aagcatcagc actaattggt
      721 agtccatcac ttttacttcc aatagcaatt ggaattatag taatagttgc agggcttaat
      781 atgttaggtt caaaaactgg aggagtcata caaactgttt ctacaatttg taagttaata
      841 ccacttattt taataatggt tgttggattt attaaaggtg gtggaaataa tccaatactt
      901 acacctatgg ttggtgaagg attaagcctg ggtagtgtac taggtcaagt tttaattgct
      961 atattatttg cttttgatgg atggatgaat gttggtactt tagctggaga aatgaagaat
     1021 cctggaaaag accttccaaa agctataata ggtgggttgt cagttgttat ggctgtttat
     1081 tttataataa atttagcata tctttgggta ttaccagcta atgaattagc aaattatgct
     1141 tctcctgcat cagcagttgc tgaagttata tttggttcaa tgggtggaaa aataatatct
     1201 gtaggtatat taatttcagt gtttggagca ttaaatggat ttttactaac tggttcaagg
     1261 gtggcatata ctttagctac agataagact ttaccaaagt atggtatatt ttctaaatta
     1321 aattctgctc aagttccagc aaatgcaata gcattagtat ctgttattgc atctatatat
     1381 gctttgtcag gtcaatttaa tttattaact gacttagctg tatttgctac ttggatattc
     1441 tatgtattaa cttttatagg ggttatgaaa cttagaaaga ctcatccaaa tataccaaga
     1501 gagtataaag ttccattata tcctattgtt cctataatag ctatagcaag tggtatattt
     1561 gttgtagtta atcaattgtg ttttgcaggt atgaaaacta ctatgatatc tataggaggt
     1621 cttgtaataa cagctatagg cttaccagtg tatgcttata tgactagagg agtaaaaaga
     1681 taaaccaagt agtttacaaa tgcttcataa ttaaaataaa agagactgtc tcaaatagag
     1741 ataagtctct tttttattgt taaaatggta aagatatgtt tgattttaca aaaaaagagc
     1801 gttccatgaa ctaaaaagtt tattttaagg tattcatttt atttttagtt ttttgattat
     1861 aaaattaaac atctaaaaaa ttatttattt taaaatactt tattttaaag tgt
//
LOCUS       DAELNY010000300         1890 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.303, whole genome shotgun sequence.
ACCESSION   DAELNY010000300 DAELNY010000000
VERSION     DAELNY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.303"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..629)
                     /locus_tag="KPE55_003635"
     CDS             complement(<1..629)
                     /locus_tag="KPE55_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0494645.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLE"
     gene            complement(999..1889)
                     /locus_tag="KPE55_003636"
     CDS             complement(999..1889)
                     /locus_tag="KPE55_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0494646.1"
                     /translation="MKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYAIYSH
                     KYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKIMPEI
                     LNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIEPNGV
                     DSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLPGTLY
                     LRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSLSSSY
                     SKIMKEKLKNSGDVQISFFD"
BASE COUNT          648 a          296 c          180 g          766 t
ORIGIN      
        1 tgttctaatg tattataatc attgtattta caattatctt gtagacacat atatttttca
       61 ttactccaaa atcctccatc ttcatttatt tcccaatatt taatttcctc aaaattattg
      121 tatttattta ttgtaaatac atctaatatt aattttcctc caggttttaa actatcataa
      181 attttttcca ttaataacct tctattttca gttgacaaag ctccataatc acaataaatt
      241 aaagtagcca aatcaaattc ctcattatag ttcatattta aataactttg gaacaagtaa
      301 tttatgttta aattagtctc cttatttcta ttttgtgcat agttaataga acgttttgag
      361 aaatcaattc cagttacctt ataacctttt tgtgcaaatt tctctgcata taaacctgga
      421 ccacaaccta aatcaagtaa tttaggataa ttagctggat ttgcaactgt aactatccaa
      481 tttacagaat cctcgataaa gttaaaattt ctacttgctc cttcaaaatt agtgtctaag
      541 tgtgctttta ataattgctt tgaaatatac tcatcatccc aaaaattaat ttcactttgt
      601 ttataaagct ctggtctttc taaatacata cataatttgt ttaacaaaac ataccactcc
      661 ttaaaattta tttttaaaat aaaaaaacca cagagtttac tctgtggttt aacttcaaaa
      721 agatgagttt aaataagagt aaaatagaac caaatatttt taagcacaac aataaaacta
      781 tagctttact ttgtttgctt attaaatttg attttattca ctcttttcca gcaatactta
      841 tatcaacgat tttatgatat caaatatcac cgaacaacat catcctttca tatttttatt
      901 ttatgaatta attatatatt taaattttat atttgtcaat aaattttaat tttaataaat
      961 tttaatcatc atagtaaaaa aaattttttt atataaaact aatcaaaaaa agatatctgt
     1021 acatctcctg agttttttaa cttttccttc attattttag agtaactgct tgatagagaa
     1081 tacttatatc taagttcatt aaccatttta tacaattgat ttttatactc cctattaagt
     1141 gaacctgcac tatatagtat tgataattta tcaaataatt caggaaactc ttttttaaca
     1201 aagtcaaaaa aaacacctct agttataccc cttaagtata aagtcccagg taaaacataa
     1261 tgtacattac attcttttgc atgtctaaat agcgaattta tattatcaaa atcatctgta
     1321 atatatggta taattggcat aatatgtaga ccaactgatg cgttagtttt tctaaattct
     1381 tttaacattt taaatcgtct cattgaatcc acaccatttg gctcaataag cctttgtgta
     1441 tcttcattta ctgtggtaat tgtagatgct acattgatat atgtaatcct agatagctta
     1501 tcaattaaat cgtaatctct taaaattaaa tctgactttg tagaaataat agctggtgtc
     1561 ttgtatttta ttagcaggtt taatatttct ggcataattt tatagtcagc ttctattggt
     1621 tgataactat ctgtaactcc tcctatattg attacttctt tcttccattt actgctttta
     1681 agttgtctct caagtttttc tactatattt gtttttacat aaatctcttc aaaataatta
     1741 ttggagttga tgtacttatg agagtaaata gcataacaat atttacatcc atgctcacat
     1801 ccacgataaa tatttaaatc ccatttatat ggcatactcc tttttaattt attacaagct
     1861 atttcacaat ttattggaat atattttatt
//
LOCUS       DAELNY010000301         1782 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.304, whole genome shotgun sequence.
ACCESSION   DAELNY010000301 DAELNY010000000
VERSION     DAELNY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.304"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            7..81
                     /locus_tag="KPE55_003637"
     tRNA            7..81
                     /locus_tag="KPE55_003637"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            87..172
                     /locus_tag="KPE55_003638"
     tRNA            87..172
                     /locus_tag="KPE55_003638"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Leu,seq:taa)
     gene            188..263
                     /locus_tag="KPE55_003639"
     tRNA            188..263
                     /locus_tag="KPE55_003639"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Met,seq:cat)
     gene            271..345
                     /locus_tag="KPE55_003640"
     tRNA            271..345
                     /locus_tag="KPE55_003640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Glu,seq:ttc)
     gene            360..433
                     /locus_tag="KPE55_003641"
     tRNA            360..433
                     /locus_tag="KPE55_003641"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:392..394,aa:Gly,seq:tcc)
     gene            439..514
                     /locus_tag="KPE55_003642"
     tRNA            439..514
                     /locus_tag="KPE55_003642"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:472..474,aa:Val,seq:tac)
     gene            520..596
                     /locus_tag="KPE55_003643"
     tRNA            520..596
                     /locus_tag="KPE55_003643"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:554..556,aa:Asp,seq:gtc)
     gene            607..681
                     /locus_tag="KPE55_003644"
     tRNA            607..681
                     /locus_tag="KPE55_003644"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:639..641,aa:Gly,seq:gcc)
     gene            691..764
                     /locus_tag="KPE55_003645"
     tRNA            691..764
                     /locus_tag="KPE55_003645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:725..727,aa:Arg,seq:tct)
BASE COUNT          592 a          269 c          346 g          575 t
ORIGIN      
        1 aaatgttcca aggtagctca acggtggagc aaccggctgt taaccggtag gctgtgggtt
       61 cgagccccac ccttggagcc aaaaaagccg aagtggcgga actggcagac gcacaggact
      121 taaaatcctg cgggacttac ctctcgtacc ggttcgattc cggtcttcgg cacttataaa
      181 acaatatcgc ggggtggagc agttggcagc tcgtcgggct cataacccga aggtcgtagg
      241 ttcaagtcct gcctccgcaa ccattaaaac ggctcgttgg tcaagaggtt aagacaccgc
      301 cctttcacgg cggtaacagg ggttcaattc ccctacgagt caccaattca attaaatatg
      361 cgggtgtagc tcaatggtag agttctggcc ttccaagcca gctgtgaggg ttcgatcccc
      421 ttcacccgct ccaaaaatgg gaccatagct cagctgggag agcacctgcc ttacaagcag
      481 ggggtcacag gttcgagccc tgttggtccc accattcgcg gcctggtagt tcagttggtt
      541 agaatgccag cctgtcacgc tggaggtcga gggttcgagc cccttccagg tcgccaaatt
      601 aaataagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa gtcgcgagtt
      661 cgaatctcgt ttcccgctcc ataataactt gggtgcatag ctcagctgga tagagcaacg
      721 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcattaaga aaagtttact
      781 ataaattata gtaaactttt atattgttta aaaagacaca ggaaacatgt atattacctg
      841 tgtcttttta tttataaact ttaaactatg taaaaattac agtatattct aatagagtta
      901 cagtatattc attaatattg taaacatacg gagggtaatg tataatagtc tacaagatgt
      961 ttatgaaggc atcagttaaa tataataaat ataactaatt agtaagagtt atatacatta
     1021 tattgtatac tttatggtat ttatatattg aaataactta tgaaattatt tataaagaga
     1081 ttcatgagca tatttttgtc taaacaataa gtataagaaa ttataaaaaa ttagagacta
     1141 tagtgaattt acaatttaat gtaatattta taagttatac taaataaatt attatactgt
     1201 gaaatggata tataacttta ggtattgtgt attattttca ctgtaggaga ttatatctag
     1261 ttatacataa taagttttaa tgatagttta tagtaagata ttaaaataaa tttatctaat
     1321 ctagatatga aataagttat tactttaata taaaaatgaa tctaatggaa taatattgat
     1381 gataagtaat atttagattt tgtatttgtt tatagttaat aagtggatta tagaatttat
     1441 aataattaat atatattaaa caaagatata ttaagatatt ttagaacata aaactgaatt
     1501 aaggcgcgag tactattttg tattcgcgcc ttgatggttt gagttatttg agtaaaaact
     1561 aactttaata agaaatatgt atttattaag ttgatatttt taatgtgttt agagattatt
     1621 taacaatctc caaatataaa actagaatta tttacataaa agttaaatag tttgtataga
     1681 gttagattta tatagtataa tacttagtgt aaataattaa gaagttatca aacaggagga
     1741 taaaactgtg ttagatataa attctttttt aaatggattt tt
//
LOCUS       DAELNY010000302         1766 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.305, whole genome shotgun sequence.
ACCESSION   DAELNY010000302 DAELNY010000000
VERSION     DAELNY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.305"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1231
                     /locus_tag="KPE55_003646"
     CDS             92..1231
                     /locus_tag="KPE55_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0494647.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFSNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1257..1445
                     /locus_tag="KPE55_003647"
     CDS             1257..1445
                     /locus_tag="KPE55_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494648.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          751 a          144 c          262 g          609 t
ORIGIN      
        1 aaattgagtt tgataattat aaaacaaata atcaaaaata gtgtaaaaat taatactttt
       61 tgttaatatt aatagataaa gataatctaa ggtggtgatt aaactgacaa tagaagaata
      121 caaaaacaaa tactttactg gaaataaaat cgtactttct ctaaatagtt ttgataataa
      181 agaactatac tatcatgaaa ctgaaaatat attaaattta ataaatgaaa tagataaatt
      241 aacatcaagt aagatattta aaacaataga agataaaaat gatttggcac tagagtctta
      301 ttattctagt gtaatagaag gtgctttttc aactagaaag attgcaaaat ctataattag
      361 aggaaagatg aaacctagta ataaaagtga atatatgatt tacaataatc atagagcctt
      421 agaatatgga ttagacaact tagataaatt atatagtcac aaatttatat atgatttgca
      481 tcatatattg ggcgaaaact gcttggattc agaagaatat gagtatagaa ctgaaaaagt
      541 atatgtatgt gattcaaaag gtgagattat acatataggg cttgaacctt tgaagatata
      601 tgactttatg agtaaattaa tagattttat ggaaaatagc aaagtaagta atttaattaa
      661 aagtgctata attcattttt actttgtata tgttcatcct tttagtgatg gtaatggaag
      721 aacatcaaga gccttatcat atctatattt gattgataaa ggatatgata ctttcaagga
      781 gttttcaata tcatatatga tatctaaaaa cagaactaag tattataagg ctatacttga
      841 tgtagaaaac aaaggaaata atttaactgt atttatagag tttatgctca agtctataat
      901 acaatctatt aatgagatgc gtaatatgca tgatagaaaa tctttagaaa gtatattaaa
      961 agaagaattg tttgaaaatg acataactct atcagcaaca gaagaacata tattaaaata
     1021 tatttgcaat aaagataact attctatgac attagaaaat tatataaaaa agaataaaag
     1081 tagatactta aaggctggaa taaaggaaat tgaattagtt gaccagttga tggaagtgtt
     1141 tagtaatctt gaagagatag agattttgtc taaagaaaaa aatatatata aagttaatga
     1201 taagtactta aaaatgcttg atatagacta atttagataa tctggagagg gaatatatga
     1261 ataataagaa aaaggcattt ttgatttctg gggctataaa cgtaatttca ttaattattg
     1321 ttttttcatt gtttcattat aatattatag aaaatagtac acaaaatatg ataataactg
     1381 taatcttaat ttttattttt gaaatgataa aaataaaaat catagagaaa tactataaac
     1441 tataaacaag aaatatcttt tctaagagca tattgtacaa tatgctctta atttagttat
     1501 atagatttaa tataaataat gtattttttg tagatgattt atgtaagatt tatgaccaat
     1561 ttgtgtatga tttatgttct ttaatcatga caatattata ttgtaaaaaa taaatttaat
     1621 aaaaaatgac ttggaattta tattctaggt cttttttata taaaaattta ataagtttca
     1681 aatgttggta actttgacag tagaaaacgt ctattgagat aacgaggatt ctaatttagg
     1741 gggagatata ttagcactta aagtag
//
LOCUS       DAELNY010000303         1705 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.306, whole genome shotgun sequence.
ACCESSION   DAELNY010000303 DAELNY010000000
VERSION     DAELNY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.306"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    447..710
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            861..1412
                     /locus_tag="KPE55_003648"
     CDS             861..1412
                     /locus_tag="KPE55_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG0494649.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          683 a          169 c          246 g          607 t
ORIGIN      
        1 aattttacta aaaataatta aaagaatatc atttttttaa tcattggtat ctatttaata
       61 atatgatgta attaaaatgg agtaatattc tgtttcaaag gaggcatata aatacaggaa
      121 tgagtattaa atgatggtta attaagtaaa tttattgata aatgtactta attaactttg
      181 aagaatttaa ttcctcatga atcaaaatat aaaattacat caacagttat tcacaaaatt
      241 aatataagaa gaattagagt caaagagcag actattagac cattaaagta attattcata
      301 gaattttgta tatgaaaaga aatgtatagt ataatttact ttttttataa aaaatagatt
      361 acaaaatata ttttttataa aaaggtattg acatttttta gaaaaaagaa tatcattatt
      421 ttaacaacaa aattcaatac aataaattaa atcctatgac aaggaagagt agttaaattt
      481 aggttcagta tagagagctg aggatggtgg aatcttagca agaaaaaatt tgatgaatgg
      541 cccttagagg aataaattga aatcatagtt ttgagagtag actttatcgt cagtcgcacg
      601 ttatagcgaa taaggtatgt aagtacctga taatgagatg tatgaaaatt gaattttata
      661 ttaattttta tataatttag gtggcaacgc ggataatctc cgtcctatta gtttaggacg
      721 gagtttttta ttttactgaa aatttaaaag atagaaggtg gtgagaaaga tgttatgaat
      781 ttcaaaagct aaagatgatg atatagtagg ttggcaaaaa aataaattga aaattaaaaa
      841 aataggagga agtacaaaaa atgaaaacaa atacaaaaaa attaacttta aatgcaatac
      901 ttttagcgat gggattatta atacatcaaa tcacaccagc tataggatta ccaatgaagc
      961 cagatgtacc actagcaatg ttatttgtaa tattggtttt aaatagagat gattataaaa
     1021 cttgtttgat agcgggtata gtaacaggaa tatttacagc acttacatca agttttccgg
     1081 gaggtcaaat tcctaatgta atagataaaa ctcttacagc aaatatagta tttttattaa
     1141 tgaatattag ttataatatg ccttttataa aaaaattagc taagaaaaca caagatactg
     1201 tagtagttgc aataataatg ccaataggaa ctcttgtaag tggaactatc tttttattag
     1261 cagctcaagc tatagttggt cttccagggg cttcatttac agctttattc ttagcagtag
     1321 tattaccagc tgttttaata aatctagttg caggaatatt tttattcaag attgttagtc
     1381 tatcaataag aagagttagt tatcaagctt aaacacgaaa taatatttta ctttataaag
     1441 cattataata actaaataac ataagaataa agtagattat aactatttta ttcttatgtt
     1501 gtttaaagat ttggtattat atatttattt ttaataaaat atttatatag taccaattac
     1561 acttaattta acagataaat tatcatacct atataatatt cttatatgta tctcatttgt
     1621 agttattaaa tataagatat atttaattga cttttcaaga taaattttta tattaatctc
     1681 ctctgatttc tcattttttg aatac
//
LOCUS       DAELNY010000304         1680 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.307, whole genome shotgun sequence.
ACCESSION   DAELNY010000304 DAELNY010000000
VERSION     DAELNY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.307"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            172..984
                     /locus_tag="KPE55_003649"
     CDS             172..984
                     /locus_tag="KPE55_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG0494650.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1073..1264)
                     /locus_tag="KPE55_003650"
     CDS             complement(1073..1264)
                     /locus_tag="KPE55_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494651.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          626 a          192 c          236 g          626 t
ORIGIN      
        1 caagaatgtt ataatttttt tattgtattc tttttgctat agtctatgaa taaatcaata
       61 gaaaatcttc taagttgctt ttaaataatg actttatggt tcaattgtac taaaatagta
      121 tatgtaggtt acaaataatc taataaatat taaagtttgg aggtaataga tatggcagat
      181 tgtaattcat gtccatcaaa gggaaattgt aatagtcaat caaattgttc tattgaaaat
      241 aaccctaaca ataaatttgg aaaaataatt ggtgttatga gtgggaaggg tggagttgga
      301 aaatcaactg taactgcttt acttgctaat aagctaaaca aaatgggata taaggttggt
      361 atattagact cagatataac tggaccaagt attcctagac ttatgggtgt aaaaaatgta
      421 aaagcttatt ctgatggctc ttatatttac cctgtagaaa actcaaataa cataaaagta
      481 atgtctataa accttatgat agatgatgaa aatgaacctg ttgtttggag aggaccttta
      541 cttggagggg ttgtaaagca attttatact gatgtacttt gggaagaatt agactattta
      601 ttaatagata tgcctcctgg tactggagat gttgccttaa cagttatgca atctattcct
      661 ataagtggaa tagttatggt ttcagtacct caagatttgg tatctatgat tgtatcaaaa
      721 gctgttaata tggctaaaaa aatgaacata aatgtccttg gagttattga aaatatgagt
      781 tatatccaat gtcctgattg ttctaaaaaa atcaagttat ttgaaggaga atctactgaa
      841 aagtttttag atgatttaga cttagagctt ttaggagaac ttcctatgac taaagaaatt
      901 atagatatta ctcataatgg agttacagaa attagtgatg atttagattc aatattgact
      961 aatgttgttg aaaaaattaa ataaataaaa attttataat tttatattta tcaaaaaaag
     1021 aggcttgtat ttattttata atcatacaaa taaatataaa cctcttttat gtttatctat
     1081 gttttaaata cacaaaatat tcttctactt catctccatt ttttactttt actaaatatt
     1141 cattataatg agcattatat ggctgtgtca ttttgcattc tattacattt tctatgtctt
     1201 ctaattctaa aagttctaca tcctttaaaa attttctttc tatgagttcc attaatcttt
     1261 ccataatata tcctcacttc ccctataatt aagataaact atatatcctc ttaatgcaga
     1321 gtttatctgt ttattatatt atatccaata tattacagtt tctaacatca ttcataaatt
     1381 attttaacaa atagagattg attttttaat aaatatgcct tgataaaatg aaacatagac
     1441 caactcttag ttttatgtaa agaaaactta aagaaaacta taaattatat tcacaaaaat
     1501 tactcagaag atataacagt aagtaatata tgtaatgaat tacaacttaa taaaagttat
     1561 tttttctttg tttaagaaaa aaagtggata tacttttact aattttttaa ataaagttag
     1621 agttgaaaaa agcaaaaagt atatattaaa aaaagacttg tctattttag acgttgctgt
//
LOCUS       DAELNY010000305         1672 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.308, whole genome shotgun sequence.
ACCESSION   DAELNY010000305 DAELNY010000000
VERSION     DAELNY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.308"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            286..1557
                     /gene="serS"
                     /locus_tag="KPE55_003651"
     CDS             286..1557
                     /gene="serS"
                     /locus_tag="KPE55_003651"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0494652.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          706 a          170 c          301 g          495 t
ORIGIN      
        1 taactatata agattgataa agaaaaagtc aaaatagata attgattagc aaaatagata
       61 taattataat gaagttaaat ctagcaatat tctcctaaaa ttaaatttca tttatttaat
      121 taaatataat ttgacaatgg gcaaaaaagt gagtaaaata taaataaaat aaaaataaca
      181 ggttcggata aagaaaaagg tttcttaacc tcaagcataa agtatattat ctaagttatt
      241 tgagattaag agataaatcc aatcttaagt actgaggagg aaaaaatgct tgatataaaa
      301 agaataagag aaaatttaga ttgtataaaa aaagcaatgg aaagaagggg cgaaaaagac
      361 tttaaccttg atgaagtagt taagttagat gatgaaagaa gaaagattct tcaagaagtt
      421 gaagttatga aaaatgaatt aaatacagca tctaaaaata taccaaatct tataaaagaa
      481 ggtaaagatg tagaaaatga aaagattaaa ctaaaagagt tatctgataa aattaaggta
      541 atagaccaaa atcttaaaga agtagaagat aagatggaat acctattaat gagaatacca
      601 aatgtaccac atccagaagt accacaaggt gaaactgatg aagataatgt tgaagtaaga
      661 acttggggaa agactactac ctttgatttt gaatctaagg ctcattggga aattggaaca
      721 gaattaggta tacttgattt tgaaacagct agtaaaataa ctggttcaag atttactctt
      781 tacaaaggtt taggtgctag attagagaga gcattattaa atttttactt agacacgaat
      841 acaaaagtaa atggatatac agaagttatt ccaccattta tggcaaatag aaatagtttt
      901 ttaggtacag gacagttacc taagtttgaa gaagatatgt ttaaaataga aggtctggac
      961 tattttatga ttccaacatc agaagttcct ttaactaata tacatgctaa tgaaatactt
     1021 aaatttgaac aattaccgat aaattataca gcttatactc catgttttag gtcagaagct
     1081 ggttctgctg gaagagatac aagaggttta gtaagacagc atcagtttaa caaagtagaa
     1141 atggttaaaa tagttgcacc agaagaatct tacaatgagt tagaaaagct tactaacaat
     1201 gcggaaacta tgctacaatt attgaatctg ccttataggg tagtaaagat atgtacaggt
     1261 gatttaggat ttacggcttc ttttaaatat gatgtagaag tttggatgcc aagttacaat
     1321 aggtatgttg agatttcatc ttgttcaaac tgcgaagatt tccaagctag aagagcagga
     1381 ataagattta aaagagataa agattctaaa gctgaatatg tacacacatt aaatggctct
     1441 gggttagcta ttggaagaag tgttgcagct atattagaaa attatcaaca agaagatggt
     1501 tctgtggttg ttcctgaagt tttaagacca tatatgggag tttctgttat taaataagag
     1561 ttaaattatg tatttttaag caatacaata atatagaggt aataaaaaat aattaatata
     1621 aaagtgatac ttctaatata gaagtaataa aaaaatagtt aatataaaag tg
//
LOCUS       DAELNY010000306         1606 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.309, whole genome shotgun sequence.
ACCESSION   DAELNY010000306 DAELNY010000000
VERSION     DAELNY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.309"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..879)
                     /locus_tag="KPE55_003652"
     CDS             complement(<1..879)
                     /locus_tag="KPE55_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494653.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYR"
     gene            complement(884..1600)
                     /gene="cas6"
                     /locus_tag="KPE55_003653"
     CDS             complement(884..1600)
                     /gene="cas6"
                     /locus_tag="KPE55_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0494654.1"
                     /translation="MESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFNYGKERKN
                     NKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKVKELEYKG
                     YRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLNYIANLTL
                     ESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGDMEDLNAL
                     YKMGIGYRRTENAGMVDILK"
BASE COUNT          507 a          263 c          126 g          710 t
ORIGIN      
        1 tctatatagt ctaattgaac ttattttttc atgcaaaagt tttttataaa atgatttatc
       61 tgaatacatt tttcttttaa gcattatctt atgatttaaa aatgctaatt ctcttaaatc
      121 attcgtatat gcaaaaacca ttggtctttt taaatttata tataaaagac acattaagtt
      181 gaaaaattca cactcaaaac aaatattact ttcaacttgt ccacttcttt taaagcctga
      241 tttttctgca cctctaaata aaaagttata cttagagtcc tttcgttctc ttaaatcaat
      301 atctagtttt gttgttttat ttatattgca aacattacat acactatctt tttcttcata
      361 ttgtgtcaaa tcaagtttat ctatttcatc tatctctata tttgatttaa ttacagtatc
      421 tactattctt tttacatttg tttccatatt ttctaaactc gtgtttaatc catacttacc
      481 actatttcta acatatggaa acctcctgtt tttaaaatct tttttaatat cttccttttg
      541 tttagcagtt gcagaagatt tatccaattc ttcaataaaa tttacatacg ttaaattatc
      601 tatattacta aatatttttt ctaaggtttc ctttttccca tttttaaata ccttaaaaag
      661 ttgattttct aatatataat cagatatata cttagggtct atatattctg cagattctaa
      721 agataaataa tttctattta actcacaagt aaattttagc ttataatcat ttttttctag
      781 ttcgtttttt aaatccatta gtaattcata aaagtaaaca atgccaatat ctcttacaat
      841 atcacctgat ctataaaatt tagcttttat ttctctcacc tcgctacttt aatatatcaa
      901 ccattcctgc attttccgtc cttctatatc caattcccat tttatacagt gcatttaaat
      961 cttccatatc tccttttaaa aaaaatgttc ctgcataagc atttatatag taaaattctc
     1021 tcactttaaa tccacgaatc ttttctttta acactctttt tttgaagtta agaggtataa
     1081 attccaatgg ctttctaagc ccactacctc ttatactctc cagagttaga ttagctatat
     1141 agtttaaaca ttctatataa tttgaatctt ctacatctaa atatttacct tctttattct
     1201 ttactattat aggagacata gttttaaata taacaccatt ttcttttatc tcgtgttctt
     1261 tatgtatttt tatatttttt attttaaatc tataaccttt atattctagc tcctttactt
     1321 ttaaaattcc attgtataat ttactgaaca tagaataatt ataaaaactt atattgaata
     1381 ttatatctcc atcaacaagt aactcatttt tgcttcttat aagatttggt atgtaaatgg
     1441 ctgtatttat atcctttatt tgcttattgt tttttctttc tttcccatag ttaaatatgt
     1501 ttttagcaaa atctttgttt tctgcattta aaatattttt tataaaggaa actgacaaca
     1561 ttgaattttc atttggaaat ttgccattac atgattccat aaaaat
//
LOCUS       DAELNY010000307         1532 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.310, whole genome shotgun sequence.
ACCESSION   DAELNY010000307 DAELNY010000000
VERSION     DAELNY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.310"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            165..1226
                     /locus_tag="KPE55_003654"
     CDS             165..1226
                     /locus_tag="KPE55_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0494655.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGAVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1239..1436
                     /locus_tag="KPE55_003655"
     CDS             1239..1436
                     /locus_tag="KPE55_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0494656.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
BASE COUNT          562 a          180 c          275 g          515 t
ORIGIN      
        1 taaaaataca aaaaaactat agaaattgac atatattcca gtatttgcta aagtagctgt
       61 tggggaaaat tacaaaaatt attaatgcgc attagataat ttgctttaat ttttttagca
      121 tttggggtaa tttataatat aaagcattag aaaggaaaat ataaatgagg gactttttaa
      181 aaaattataa gtcatcaatt atacttttag catgtactat agtaggggcg atagtaggat
      241 tagtacttgg tccaaaagct tcaatactaa aaccatttgg agatttattc ataaatgctc
      301 tattaatgat agtagtacca ttagtttttc ttagtgtttc ttcaggtgtt gctagtataa
      361 aaaatatgga acgatttgga aaaataatga agggtacagc tatagtattt atgggaacaa
      421 cacttgtaat tgcattttta ggtattacat ctgcattatt atttaatccg tctaaaggat
      481 tagatgtatc aagtgtaaaa aatttaatgg tggctcaaga tgcagaaact ttagaatcaa
      541 aaggaacgtt attagaccaa gtagtatcaa gtattacagt taaaaatttt ggagatttat
      601 tttccagtag taatttacta caattagtta tatttgcagt tttaattgga acagcaatag
      661 ctatgatggg agatagggga aatacaataa gaaagctttt atcagaagga acggaagcaa
      721 tattaaaagt agtaagtata ttcatgcttt atgctcctat agggctaggc tgttatacag
      781 ctacaataat tggggaactt ggaagtaaaa taattcaagg atatgtaagg gtgtttgtaa
      841 tttattcact aacagcagcc atgtatttct tattatttta cagtctgtat gcttttttat
      901 ctgcaggaaa acaagggttt agacaattct ggagaaatgc tataaaacca atagcaacgg
      961 cactagctac ttcttcaagt tcagcatgta taccatcaaa tttagaagct agtaaaaata
     1021 taggagttcc tgatgatata tcagaaacag ttatacctct tggaataaat atacataaag
     1081 atggggcagt tttagcagta ataataaaag tggcattttt atcaggtatt ttaggtagtg
     1141 gaatggcaaa tccagcaacg ataggtcaat tatttataac tgtatttgtt gttggagtat
     1201 ttatggctcc tgtagcaggt ggctgactta ttggtgagat gctcgtggtt agcatcttcg
     1261 gttttccagt ggaggcaatt ccactgttaa cagttataac tactataact gatattccta
     1321 atactatatt aaatacaact ggaaatacag tatcatcaat gttagtttca agattagtag
     1381 aaggaaaaga ttggcttata gataaaacag caattactac aaagaaaata agctaattgt
     1441 acacttaatt aataaagtta ataggtatct taattttaat aaattagata cctattaact
     1501 ttttaagtaa aaatataaat ttaagattca aa
//
LOCUS       DAELNY010000308         1504 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.311, whole genome shotgun sequence.
ACCESSION   DAELNY010000308 DAELNY010000000
VERSION     DAELNY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.311"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            361..1350
                     /locus_tag="KPE55_003656"
     CDS             361..1350
                     /locus_tag="KPE55_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG0494657.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSSGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          574 a          146 c          260 g          524 t
ORIGIN      
        1 aatattagtg ataataagct tacaatttat cttacattaa aaaatttaag tggatttttc
       61 aaaatataga taaatttctt tttcattgta taaatgataa aaatatattt gacttcatac
      121 caaaaagagt gccccatgaa ctaaaaagtt aattttgaaa taccatattc atatatcata
      181 tgatttattt ggaaatcaat attaatacat taaaataact aaatacatgt atgcataaag
      241 atgtataaaa aatatataaa catatagtat taattttaaa ttcaacaatt taattgttaa
      301 aagtgttatt attaaattag aaatatctta acttaatata ctgagatggg agtgatagat
      361 atgttaaaag aagaaaaaac ttatcaagag aaagacgcaa aagtattcaa tggagtggca
      421 gctttgataa tgattttatt atttttagta gtttcaatag gtatcatggt ttttgggata
      481 gtaaaacttg atagtggatt tactggaata ggttcactta tgttaatttt gggacttgta
      541 tttatagtag tagattttat cctatttttt gggctaaaaa tgattaatcc taaagaagct
      601 atagttctag ttttgtttgg taactattat ggaactataa aaaaagaagg gtactattgg
      661 gtgaatccat tttgttcagc aataaaccca gcagtatcaa gaattaatgt atcgaaatct
      721 tcatctgatg aaaaggtaga tatatcaact aattcaagtg gcaaaaaagt atctttaaaa
      781 acaatgactc ttaacaatga aaagcagaaa gtcaatgatt tattgggaaa tccaattata
      841 ataggagtgg ttgtaatttg gaaggttata gatgcaacaa aggcagtatt taatgtggat
      901 aattataata cattcttatc tattcagtgt gattctacta ttcgcaatgt ttcaagactt
      961 tatccatatg atgtatctga agatggagat gaaaaatctc ttcgtggtag tagtcaggaa
     1021 atagcagata gattaaaaga tgaattacaa tcaagggttg atattgcagg aattgaagtg
     1081 tgtgaagtga gaattactca tctttcttat gcaccagaga ttgcagcagc aatgttacaa
     1141 cgtcaacaag cagaggctat aatagcagca cgtaaaaaga ttgttgaggg ggctgtaagt
     1201 atggtagaga tggcattgac taatttaagt gaaaataatg ttgtgacttt ggacgaagaa
     1261 cgtaaagcag ctatggtaag taatctgtta gttgtgcttt gtggcaataa agatgcacaa
     1321 ccagtagtaa acagcagttc tatttattaa attataaaaa gtttaatagt ttattttatt
     1381 gatatataga aaatttagta gttcatttta ttaaattata gaaagattaa tggtttgctt
     1441 tattgaatta taaaaagttt aataatttat tctattgata tatagaaaat ttagtagttc
     1501 attt
//
LOCUS       DAELNY010000309         1492 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.312, whole genome shotgun sequence.
ACCESSION   DAELNY010000309 DAELNY010000000
VERSION     DAELNY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.312"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..399
                     /locus_tag="KPE55_003657"
     CDS             154..399
                     /locus_tag="KPE55_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG0494658.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKLLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(617..886)
                     /locus_tag="KPE55_003658"
     CDS             complement(617..886)
                     /locus_tag="KPE55_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0494659.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          603 a          214 c          168 g          507 t
ORIGIN      
        1 actcagtgat ttagaggtgg taaaatatat atatatgaaa gagcataaat aaattgatat
       61 aataaaaaat taaatattaa atattaacta taataaaaaa atatctggta ataggtataa
      121 tataaaaaga atataccaga aagaaggtga agtatgggag aactacaaaa aattccaggg
      181 gtagggaaag caacagaaaa atctttaatc atgttaggat atacaactat taaattactt
      241 aaagatgcaa acccagcaca aatgtatgaa aaggaatgtt taatgcgtgg acagcacata
      301 gacagatgtc agttatatgt atacagatgt gcagtatatt ttgcatcaac tgaaaatcca
      361 gaaccagaaa aattaaagtg gtggtattgg aaagattaaa aaatcaaaaa agttagacat
      421 aaaaaagagt tgaatatccg ttttaaataa taaaaactaa tactcaactc ttttttagtc
      481 gtaaatttta tgtaaatcta tttgtatggc taaatatacc catcaataaa tgtataaata
      541 taccaatcaa caaatgacta aacatactac ctaacaaagg attaaataaa ttacttaaca
      601 aagcattcta aaacaattaa ttagaaactg tttcttcaag ccattttcta cattgctcaa
      661 tatttaccat agggtcaaaa ccttggtctt ttcccttatt taaaagcata tcacaaggga
      721 aagtatcaca ttcaccacaa tgattaagtt ccttactttc acagcaagtt ttaaccttac
      781 attctccacc ccaaaatggt ttttccatag ttgggcaacc agtgcaattt acttgttctt
      841 tttttgtaca actgtcacaa cagacaccac atcttgattc aaacatgttc aaacatcctt
      901 tccattattt aaaaatatta taaaatgatt ttagatgaat acaaaagtgc aaatagttga
      961 ttttaggtca tctaaatgtt aaacataaaa ttattagtac tggtaaattt ataaaaacta
     1021 ttccaaaaat ctttttgaat atttcaaaac acaataaatt acttcaaatc cctttgaata
     1081 tattttacag cattatagat tgctttaaat ctctttgaat attctacaag actatagatt
     1141 agtttaaatc cctttgaata tattttacaa cattatagat tgttttaaat ctctttgaat
     1201 attctacaag actatagatt agtttaaatc cctttgaata tttcaaaaca ccatagatta
     1261 ctttaaatcc ctttgaatat tctataacac tataaattac ttttaacttt tatataacca
     1321 taaagttttt atctggcact ttaaatggag ttttatatca aatttgcctt ccatcgaata
     1381 accaaactta atttaattac acattccaag agtcaaatat tcattcaata tatttgataa
     1441 ttctatgtta tagctataat taaatataga ttattaaagt taaatattta tt
//
LOCUS       DAELNY010000310         1476 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.313, whole genome shotgun sequence.
ACCESSION   DAELNY010000310 DAELNY010000000
VERSION     DAELNY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.313"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            281..>1476
                     /locus_tag="KPE55_003659"
     CDS             281..>1476
                     /locus_tag="KPE55_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG0494660.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATN"
BASE COUNT          618 a          181 c          169 g          508 t
ORIGIN      
        1 atatcatatt tctagtatgg taaattgtgt atttcactaa taatatatta acaagttaac
       61 ttaatgtaga ttattctttc ccatttgtta taataatata atataaagag ctaataaagt
      121 tattttaata acatttaacc agttttttat gtaggtaaca tttctacatt tttataaatt
      181 tttttacgtt ttttaatttt ataattaaaa atttaaattt tatacatatt ataataaatt
      241 gtgatatata taaaataatt aaagataaaa ggagtatttt atgaacatac ttggattaat
      301 agttgaatat aacccatttc ataatggaca catatatcat ttatctaaat ctatagaaaa
      361 aacaaatgca acacatactg tagcaataat gagtggaaat tttctacaaa gaggcgaacc
      421 agctttattt gataaataca ctagagcaaa aattgctgtc gaaaatggtg tagacttagt
      481 tatagaatta ccaactttat ttgcttgtca aagtgctgaa attttttctc atggtgctat
      541 aacaatttta aattcactaa actgcataaa ttctatatgt tttggtagtg aagaaggaaa
      601 tacagaaatt ctttataatc tagctgaaat tttagcaaat gaaccaaatg aatttaagtt
      661 acttttaaaa aaatatttag atgatggtct gttatttcca acagctagag ctaatgcact
      721 ttttgattat atacatcaag acaaaaaaaa taattttata caaaattatt caaaggaaca
      781 attatcaaaa acattaaatt catcaaacaa catactgggt atagaataca taaaaaacct
      841 catccattta aacagtagta taaaaccatt tactatctcc agggtccaat cagaatataa
      901 ttccgaagaa attgagagtg atatttgttc tgctactgct ataagaactt cacttaaaga
      961 aaatacagac ctttttcatt taaaaaatgt tattccagaa aaaacttata acatcctaaa
     1021 tgaaaaaata gataatggtt ttttacctat gtttgatgac ttcttctttg atattttaaa
     1081 aacaattata cttagagata tggacacttt aaaagagtat tttgaagtaa atgaaggcat
     1141 agaaaacaga atctataaag atatctttac atctgaattt ctacatgaac tacaattagc
     1201 aattaaatcc aagcgataca ctttaaccaa aatcaaacga actctaaaca atatattgtt
     1261 aggtattaaa aaaagcgacg tgcaacttat aaaaaacata gatactatac cttatataag
     1321 aatacttgct tttaatgata aaggtagaga aattctaaaa gagataaaaa ataaatctga
     1381 aataaaaata ataaacaagt tttcaaatat atcattttca ttagatgata cgatttttaa
     1441 aacactcata agttatgata taaaagctac aaatat
//
LOCUS       DAELNY010000311         1459 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.314, whole genome shotgun sequence.
ACCESSION   DAELNY010000311 DAELNY010000000
VERSION     DAELNY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.314"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..1214
                     /gene="ugpC"
                     /locus_tag="KPE55_003660"
     CDS             105..1214
                     /gene="ugpC"
                     /locus_tag="KPE55_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG0494661.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1244..>1459
                     /locus_tag="KPE55_003661"
     CDS             1244..>1459
                     /locus_tag="KPE55_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBG0494662.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKN"
BASE COUNT          606 a          185 c          216 g          452 t
ORIGIN      
        1 ctaaatatac atattaaaca aatacattta gtcattttac acatttaata taacaaaaaa
       61 atttgatact atataattaa aatttaaata tcaaggagga tattatgtca gaagtaattt
      121 taaaaaatat atcaaaactt tactcaaatg gatttaacgc agtaaaaaat ataaatatag
      181 acattaaaga taaagaattt atcgttttag taggaccatc tggttgtggt aaatcgacta
      241 cacttagaat gattgctggt cttgaagaaa tctctgaagg agaactatac attggagata
      301 aacttgtcaa tgatatagaa ccaaaagata gagatatagc tatggttttt caaaactatg
      361 ctttatatcc ccatttatct gtttacgaaa atatggcatt tgcacttaaa cttagaaaat
      421 tacccaaaga tgaaatagac aaaaaagtta aagaagctgc taaaatatta gatttactac
      481 ctcttttaaa taaaaaacct aaaacattat ctggaggaca aagacaaagg gttgccttag
      541 gacgtgcaat agttagaaat cctaaggtat tcttaatgga tgaacctcta tccaacttag
      601 atgcaaaact tagaactgct atgagaacag agataactaa gctacaccaa cagcttggaa
      661 ctacatttat ctatgtaaca cacgaccaag ttgaagctat gactatggct gatagaatcg
      721 tagtaatgaa ggatggagta gttcagcaga ttgcaactcc acaagatgta tacgattatc
      781 cagctaatat atttgttgca ggatttattg gagcaccaca gatgaatttt attgatgtaa
      841 tattaataga agaaaatgat gaaatttatg cacaaaatga acatattaag cttaaattga
      901 ataaagaaaa acactatgat ttaataaaag acaattatat taataatgaa gttgttatag
      961 gtatcagacc agaagatatt catgtagaag atacttttat taaatctaat ccagatactt
     1021 gttttaaatc aagaattgaa ataaaagaac ttatgggagc agaaacttat gctcacttaa
     1081 aactaggcga aaatacgata acaattagat ttgacagtaa aaacagaatt aatgttggtg
     1141 atgacttaac actttcagtt gacaatagta gagtacacat atttgacaaa gaaactactt
     1201 tagctataag ataagaatat aatcttattt ttaaggagac tttatggaag agttaaaaga
     1261 ttttttagaa agacagctta ataaaaaaat aaatatctat ccatatgaaa accaaaagtt
     1321 agatgctagt agccatttag ttactttcaa taatgctatt tatgttattg attttctaga
     1381 taaaaataat ctcgataatt taaaaggcaa cttttattta atttaccaat ctcatataga
     1441 ctttgaatat ctaaaaaat
//
LOCUS       DAELNY010000312         1451 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.315, whole genome shotgun sequence.
ACCESSION   DAELNY010000312 DAELNY010000000
VERSION     DAELNY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.315"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..611
                     /locus_tag="KPE55_003662"
     CDS             114..611
                     /locus_tag="KPE55_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0494663.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            660..1184
                     /locus_tag="KPE55_003663"
     CDS             660..1184
                     /locus_tag="KPE55_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0494664.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELKKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT          598 a          115 c          249 g          489 t
ORIGIN      
        1 ataaaaataa atttattgac ataatagtaa ctaattacta taatataaag taattagtta
       61 ctatttaaaa ttatcaaaaa attaaaatct aagataaaga gaagtgtata ataatggatg
      121 cagaaagttt aatgaaaaaa tatatggtag atgatgatag tagaaaacaa gctatatttg
      181 caagtatatt tgtaatgcaa aataaacttc aaactacatg tgataaatta gacccagaat
      241 taacaatgaa acaatggtta cttttagcta ttgcggactc tgtagatgaa actcttacat
      301 taaccaaact tggagaatta atggggtgtt caagacaaaa tgtaaaaaaa ctagctattg
      361 ctttagaaaa gaaaggtttt atagaaattg agcaaaatga aaaagattca agagctatct
      421 gtcttgtgac aaaagataga gtgcaagagt attctaaaaa agtgagaggt atgcaggaaa
      481 aagtattaca gcttttattt gaagatttta cagagaaaga agcggaacag ttattttttg
      541 gaattatgaa attaagtata ggaattgaaa atgtgaaaaa ttatgtagaa aataatgacg
      601 ttggagaata agcaagtatc aaatcaactt tgtatgacat cagagagggg agaatttata
      661 tgaaaagtgt agtagtgatt tataagagta agtatggttc aacaaaaaaa tatgctaaat
      721 ggctttcaga attattgtct tgtgatattt ttgaaactaa gaatattaat atagaaaagg
      781 ttatgaagta tgatacgatt attttaggag gaggtcttta tgcttgtaat atagcgggaa
      841 tttctttctt aaaaaaaaat tataaagaat tgaagaaaaa aaatttagta gtatttggcg
      901 taggagctgc tccatatgat gatgaagtaa taaagaaatt aagagaagta aactttaagg
      961 atgaattaaa caaaattcct tgcttttatt gtcgtggggc atgggatgag acaaaaatgt
     1021 cttttataga ttatactctt tgtagttttt ttaaaaaatc tatagctaaa aaagaacgtc
     1081 aaggtcttaa accagcagaa gcacctttat tgtctggaga agcctttgat tggtgtaata
     1141 aagaaaattt aactccaata cttgattata taagagaact ttaagaggta tatatttgta
     1201 attatttatt atctaaagtt agatttcata gaaatttact agacattatt tataaaataa
     1261 tattattaaa gatggataat ttaattgttt caataaggtt ataattttaa aaaatgttta
     1321 ttttaaaata aattatttta taagcttact tatacttaat aaggggcttt ttttattgga
     1381 agatttatgt tatattgaga tgaatttatt atgatataaa aaataatgtt aggataaatt
     1441 taaatctttt t
//
LOCUS       DAELNY010000313         1356 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.316, whole genome shotgun sequence.
ACCESSION   DAELNY010000313 DAELNY010000000
VERSION     DAELNY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.316"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1280
                     /locus_tag="KPE55_003664"
     CDS             <1..1280
                     /locus_tag="KPE55_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0494665.1"
                     /translation="DINEDIYGKYIEDIFVDSLKVLLSEKEILDKVVVFNKKYINVNK
                     KNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFKDIKTNSPKMFECI
                     DLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVNCAAVPENLLESQL
                     FGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGS
                     HNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPLRERKEDILIIMED
                     LINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNK
                     NTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQITEGKIKKIFEYL
                     KKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT          612 a          145 c          203 g          396 t
ORIGIN      
        1 tagacataaa tgaggatata tatggaaaat acattgagga tatatttgta gatagtttaa
       61 aagttttatt gagtgagaaa gagatactag ataaagttgt cgtattcaat aagaaatata
      121 taaatgtaaa taaaaaaaac atatacaata gagatgaaaa aatgggcaca tattatagtt
      181 tacaagaaat aacatacata aaaaaattag aacaaaatct cactaaaaaa ttaagagaaa
      241 aaggacaaat agcaaaatat acatttaagg atataaaaac aaatagtcct aaaatgtttg
      301 aatgtataga tttagctaag aaagtttcta aatctgattt aagcatactt atcagaggtg
      361 aaagtggaac tggtaaagaa ctcatagctc agtctataca taacaactca aatagaaaaa
      421 atcaaccctt tatagcagta aattgtgcag ccgttcctga aaatttatta gaaagccagc
      481 tattcggata tgacaaggga acttttactg gaggtctaaa agatggaaaa caaggattat
      541 ttgaacttgc taataatgga acaatattct tagatgaaat aggcgatatg cctttagaat
      601 tacaaacaaa actacttaga gttcttcaag agaaacagat aatgcctgtt ggttctcaca
      661 atgtgattaa tatagatgtt agaatcatat ctgctacaaa taagaattta gaacaaatga
      721 ttgataatag ccaatttaga gaggatttat attatcgatt aaatacaatt ccaataaata
      781 tacctcctct aagagaaaga aaagaagaca tattaattat tatggaagat ttaattaata
      841 agaaattagt aatcacacca gaagcaaaaa aacttataca aaactatatg tggaaaggta
      901 atataagaga actccaaaat gttacatcct atttaaacat tatgtgtgaa gatattgtat
      961 tagagaaaga tttacctcca aacttaaggt cttcagataa caaaaataca tcattaaaac
     1021 taaaatatag taagaatgat atattgaata tattagagat actaatttta aataaggaat
     1081 cagatgttgg tattggtaga ggattaattt taaaagcact actcgataag aaccttcaaa
     1141 ttacagaagg aaaaattaaa aaaatatttg aatatctaaa aaaagaagaa cttataattt
     1201 gtagttcagg aagatatgga agtaaaataa ctcaaaaagg agaagatttt tacaataaat
     1261 taaaatataa aggtctataa aattataatt tatcaaacaa agaaatctat tcaggattaa
     1321 agtactcctt caataggttt ttttattttt caagta
//
LOCUS       DAELNY010000314         1349 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.317, whole genome shotgun sequence.
ACCESSION   DAELNY010000314 DAELNY010000000
VERSION     DAELNY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.317"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..>1349
                     /locus_tag="KPE55_003665"
     CDS             2..>1349
                     /locus_tag="KPE55_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG0494666.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMISAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKV"
BASE COUNT          532 a          156 c          232 g          429 t
ORIGIN      
        1 aatggatata aaagagttag ttaaaatgca aagaaaatat tataatacag gtaaaacaag
       61 agatatatcg tttcgtatag aacagttgaa gaaacttaag ttagttgtat cacaaaatga
      121 agaaaaaata cttttagcac taaagaaaga tttaaataaa tcggattttg aaggttttat
      181 gacagaagta ggcatgtttt atagtgaatt aaactttgct ataaagaata taagaaaatg
      241 gagcaaaata aaaagagtta aatcaagtat ggttaatttt ccatctatat ccaaagttgt
      301 tccacaacct tatggtgtta ctctcataat gtcaccttgg aattatccat ttcaattagc
      361 acttatacca ttagtttggt cattagcagc agggaattgt gttatattaa aaccttctga
      421 atattctgtg tcaacatcta gtgtagttaa agacatagtg gaagatactt tttcaaaaga
      481 atatgttgca gttgttcaag gaagtcaaga agaaagtgaa aagttactat tggaaagatt
      541 tgattatata ttttttacag gaagtactaa tgttggtaaa ataatcatga aatcagcatc
      601 agagcattta actcctatta ctttagaact tggaggaaaa agtccttgta tcattttaaa
      661 agatgctaat atagacctta ctgccaaaag acttacttgg ggaaaactaa taaatgcagg
      721 acaaacctgc gtggctccag attatgtttt agtgcatgaa gatagaaaaa atgagttaat
      781 agagaaaata aaatattata ctaataagta ttttggagat aatccatgta ataatgagca
      841 atttcctaaa atcataaatc agagacattt taatcgaata ttatctctta ttgataaaga
      901 taagatagtc tatggtggaa attataataa ggagacatta aagatagagc caactatagt
      961 agacaatgtt aattgggatg ataatataat gaaggaagaa atatttgggc ctatatttcc
     1021 cattttaaca tataaagatt tggatgaagt aatacaaaaa attatacaaa tgccaaatcc
     1081 attggcactt tatattttta caaaaaataa gtacctagaa aataaacttt tagaaatgat
     1141 ttcagcagga ggatgttgta tcaatgatac agttactcat atagctacga attacttacc
     1201 ttttggtggt attggtgaaa gtggtatggg ctcatatcat ggaaaagctg gattcgatac
     1261 atttactcac tataagagtg tgttaaaaaa attaaatttg gatgtcccta ttagatatgc
     1321 tccatacgat aataaattaa ttaaggttc
//
LOCUS       DAELNY010000315         1336 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.318, whole genome shotgun sequence.
ACCESSION   DAELNY010000315 DAELNY010000000
VERSION     DAELNY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.318"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPE55_003666"
     tRNA            27..101
                     /locus_tag="KPE55_003666"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPE55_003667"
     tRNA            116..200
                     /locus_tag="KPE55_003667"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPE55_003668"
     tRNA            210..293
                     /locus_tag="KPE55_003668"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="KPE55_003669"
     tRNA            323..399
                     /locus_tag="KPE55_003669"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="KPE55_003670"
     tRNA            407..482
                     /locus_tag="KPE55_003670"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="KPE55_003671"
     tRNA            570..658
                     /locus_tag="KPE55_003671"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="KPE55_003672"
     tRNA            662..737
                     /locus_tag="KPE55_003672"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="KPE55_003673"
     tRNA            744..817
                     /locus_tag="KPE55_003673"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="KPE55_003674"
     tRNA            832..908
                     /locus_tag="KPE55_003674"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="KPE55_003675"
     tRNA            932..1008
                     /locus_tag="KPE55_003675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="KPE55_003676"
     tRNA            1016..1092
                     /locus_tag="KPE55_003676"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="KPE55_003677"
     tRNA            1101..1176
                     /locus_tag="KPE55_003677"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="KPE55_003678"
     tRNA            1184..1257
                     /locus_tag="KPE55_003678"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="KPE55_003679"
     tRNA            1264..1335
                     /locus_tag="KPE55_003679"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttattttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAELNY010000316         1253 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.319, whole genome shotgun sequence.
ACCESSION   DAELNY010000316 DAELNY010000000
VERSION     DAELNY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.319"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            240..1067
                     /locus_tag="KPE55_003680"
     CDS             240..1067
                     /locus_tag="KPE55_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0494667.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT          483 a          132 c          201 g          437 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca ttt
//
LOCUS       DAELNY010000317         1242 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.320, whole genome shotgun sequence.
ACCESSION   DAELNY010000317 DAELNY010000000
VERSION     DAELNY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.320"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            123..1163
                     /locus_tag="KPE55_003681"
     CDS             123..1163
                     /locus_tag="KPE55_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0494668.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          499 a          127 c          238 g          378 t
ORIGIN      
        1 tttattaagt tttgttttaa aataatatta ttaaattata actaagcttt tctgttatga
       61 tataattaaa tagataatgt gaatgattat tatatagaat acttatttgg agttgatatt
      121 ttatggacaa acacagtgat tcagtaatgt atggtggaat ggaagtatat aatatttggg
      181 gaacaacaac agtagactcc ataattggag agtctgatgt aatgcgtgct cttaagaata
      241 gaatttgtaa aattggaaat agtgattcta ctgtagctat aactggagaa agtggcactg
      301 gaaaagaatt aatagcaaga gccattcatc tcgcaggaaa cagagggaat aaaacattcg
      361 ttgctataaa ctgtgctgct ataccagaac ctttactaga aagtgaactt tttggatata
      421 caaaaggagc ttttagtgga gcagattcaa aaggtaaaat tggtaagttt gaattggcca
      481 atggaggggt tgtattttta gatgagatag gagatatgcc aattcaacta caatcaaagt
      541 tattgagggt attacaggaa aagaagttta cgagaatagg ttcaaatgaa ctaattgata
      601 tagatgttag aatcatatca gctactaata gagatttatt tgagttggtt aaggaaaata
      661 aatttagaga agacttatac tatagattga atgtaatacc tttagaagtg cctccactta
      721 gagaaagagg agacgatata caaatattaa taaacaattt tatagacaaa tattcgagaa
      781 agatgaagaa aaacgtgtac catatagagc cagatgtgga acaaatgttg cttaagtatc
      841 cttggccagg aaatattaga gagttggaaa atactataga actggcatta aaccttcttg
      901 aggaagatgg aataatacat aaaggaataa taaatagaaa gatacttgaa tattttaagt
      961 taaataatat aaatataaag ttacttgagg aaagtgacta tgagataagt atggaacaag
     1021 acatacttac acttgaagag tttgaaagag cctatataaa gaaagtgtta aagttttacg
     1081 gaaatgatac taagtctaaa aaactggctg ccaaaaaact tggaatatca ctagctacat
     1141 tgtacagaaa aatagatgta taataattag gctttttatt ctcaattcta aaatttgaaa
     1201 ttataaaatt gataaaaaac acttggaaaa ctattttcta ag
//
LOCUS       DAELNY010000318         1240 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.321, whole genome shotgun sequence.
ACCESSION   DAELNY010000318 DAELNY010000000
VERSION     DAELNY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.321"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..627
                     /locus_tag="KPE55_003682"
     CDS             <1..627
                     /locus_tag="KPE55_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0494669.1"
                     /translation="IIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIISSLYINIG
                     ANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSNLDNM
                     NVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANLLSFFVL
                     YDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKIR"
     gene            921..>1240
                     /locus_tag="KPE55_003683"
     CDS             921..>1240
                     /locus_tag="KPE55_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG0494670.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLE"
BASE COUNT          504 a           92 c          162 g          482 t
ORIGIN      
        1 attatagaaa tatggtcatg taaaaaagtt tttgattata ctagtcagtc aaagaccgac
       61 ttaaaaaaac taaatatgta ttttctcttt tctgtttttc tcattatttc ttctttatat
      121 ataaatatag gagctaatag tagaatcttg atatgtatat ttgtaatgtt cgttttctat
      181 aagtctaact ataaggttaa tatatataaa tgtctggtaa tctcattttt ttattggtta
      241 atattaatag gtataaatgg attaagtatg ttattagtag caacagtaag caatttggat
      301 aatatgaatg ttttacttag tggcagttta tatagatatg aagcaatatt cttgggaaag
      361 ataatgttag gtatattact ttatgtttat aaggcttctt ttaaaaattt taaaataagt
      421 aaaaaagaga tatacatatg tattcctatg atagcaaatt tattgtcatt ttttgtactt
      481 tatgattata ttattaatct tgtaaaaatc tctcctgaaa gaagaactgc ttttttagta
      541 atagttttac tattaatatt atctaacatt tcaatctttg tattaataca tataatactt
      601 aagattaaaa ttaggaaaat aagataaatt ttaatacgat tcatttaaat taaaagttaa
      661 taattacttt tttatgtata attttacatg aatttctata aaagttattt tattaaagat
      721 agatatactt atatatacgg atataattaa aaaaagtctt tttaacttta aaaattaatg
      781 ttaaaaaata aacttataca ttaatattaa taataattat ttaaatatct taagtaaatc
      841 agtcttacat atatttccga tagattatat tgataaaaaa cactaaaatt aaattaataa
      901 aagtacttgg agggatggaa atgaaagagg aaaattttat taatgttgaa tacgaggata
      961 aacgtttttc aattaatagt aaagaagttt tgtacataga aaagcaagag tatgaagtaa
     1021 agatttatac tgaaagtggt aattatgaag ttggagaaaa tgctccttgt ataagaaaac
     1081 tattgaaaga agaaaatttt ttcaaatgca gtaatagtta tattgtaaat ctagacaagt
     1141 gtgaatttta tctcagcaat aattgtattt ttgtgaataa taattttata agaattttag
     1201 gttcagaaag aaaaaagaaa ttaaaaaaga aattagaaaa
//
LOCUS       DAELNY010000319         1238 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.322, whole genome shotgun sequence.
ACCESSION   DAELNY010000319 DAELNY010000000
VERSION     DAELNY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.322"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..774)
                     /locus_tag="KPE55_003684"
     CDS             complement(<1..774)
                     /locus_tag="KPE55_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494671.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKL"
BASE COUNT          495 a          188 c          157 g          398 t
ORIGIN      
        1 tagttttaag tcagttgata acattcctaa actcattgca aagtatatag tgaattggta
       61 tataaaagag ccaacaatta ttgcagtagt atctttgata aaagtaaatt tcttaaataa
      121 tgtaatacca attatgatag aggcaattcc aagaactaaa gttcctatac ccatatttac
      181 atctgagaat cccaagaact gagccataag actaccagat aaagctataa gtccatttga
      241 aatcataagt cctagtattt ttatagaacc tatatttata cctagagatt taatcatttg
      301 agagttatca ccaacacctt ttaaagtgta tccaagacct gtttttagga ataaatctaa
      361 aaggacttta cagatgaata caaaagctaa ggctaaaact ataggagata tttccccatt
      421 gaataagtgt ttaaagctaa atagaggaat atttgatttt cccattattc ttaaattgat
      481 agagtatagc attcccataa ctaaaatacc agaaagtaaa ttagatatct taaacttaat
      541 gtgaagaatc cctgtaacaa gaccagcagt acaaccacat aagatagcca ttaaagttgc
      601 tactacagga gaaataccat tagtaagaga aaatgctaca atagaagcac ccattgtata
      661 gctaccatca gcagacatat ctggaaaatc taagattttg taagttatat aaacccccag
      721 tgccataatc gataaaatta aactttgcgt cattacagat ataatacctg acataaataa
      781 aacacccctt tgaaaatatt ttaaatggtt ttttaaattt gattttaaaa aatgattttg
      841 atataaaaaa accacacagt atatataact gtatggcaaa aattcgaata agccacacag
      901 tataatccat gtggctctat attatgatga taaaataaaa actttaacaa caattactat
      961 agccaacata gatacaatcc ttaaaaatca taatcctaag gggcttgtat ctatgttatg
     1021 acaatttgtc ttaacttagg tacaaaccct aactaaattg gctactaaaa aatcgtactt
     1081 gtttgtttaa taaagtcttc atatttacct ctccttttaa tttaataatt ttgaaaattt
     1141 tgttttctgt tttgtatagt aattagttta atacagtaaa aaataaaagt caactataaa
     1201 ttttgaaaaa ttatcaaatt atcagaaaat tgagttta
//
LOCUS       DAELNY010000320         1131 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.323, whole genome shotgun sequence.
ACCESSION   DAELNY010000320 DAELNY010000000
VERSION     DAELNY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.323"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..733
                     /locus_tag="KPE55_003685"
     CDS             2..733
                     /locus_tag="KPE55_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494672.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(890..1096)
                     /locus_tag="KPE55_003686"
     CDS             complement(890..1096)
                     /locus_tag="KPE55_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494673.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          471 a          122 c          141 g          397 t
ORIGIN      
        1 tatgttaagt aaaaaaaatg taagtttaat tattttagta ataattttaa gtttattaac
       61 tgctatattt attttcaata ataaaaatac taacaataaa ttgaaagaag ttactaagaa
      121 taatgaagaa atagtaccta aaaacttaga aggtttaagt atagaacaat taatagagat
      181 tagagataaa gccattgata aaaatttaga aggtcttagg gaatttggag aagattcagt
      241 aaaaaaacgt aaagattcaa ttttaaactt tgaatttgga ttagtagctt ttgttaacaa
      301 gaattatagc aacttaacag aaaaagagaa attaataaga caaattaata ttgttaacta
      361 tgaaaactct ataaatgata taatggatcc ttcaggttca ttctttgaat tagcaaatca
      421 gaattaccaa gtgaatgagt ttctattaaa aaacggaaaa gattataaag aatggtcata
      481 tgaaggtcta gattttctaa ttgatcatct agaagaactg tgcaaatttg ttgattttga
      541 tgtaaaagat gtaatagata ttattgatcc tacaataaaa acagaccttt cagaggaaca
      601 aaagaaatct ttgaatgata aattaaagaa aatatcttca agtgatactt taagtgaaga
      661 agttaaaaag gagataaaaa actgggaaaa caatttaaat aatttaaata tgaataaaaa
      721 taattctaat taattttctc ataagaaaaa tgcttatatc gtgataatgt gaaatgtaca
      781 taattataaa caaaaaaggg gctgtgacac taaaaaatag tgctacgccc ttttttattt
      841 gtaataatca tcaatacaaa ttattttatt atatgtaatt atttatgtct taaccaattt
      901 tatcgaaaat aacagtcttc tcttttaaag agatttttcc tacttgatat ccatattcat
      961 ttaattcctt tttatatttc aaaaacgaat ggtctatctc tatcccagca atatctttta
     1021 tttcttcaaa agttaattta aacgattgac tattgttttt cttaacatat tcccataaag
     1081 aactatattt actcatctta tatcactcct tttaaaatca tatcttatct a
//
LOCUS       DAELNY010000321         1090 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.324, whole genome shotgun sequence.
ACCESSION   DAELNY010000321 DAELNY010000000
VERSION     DAELNY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.324"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            269..445
                     /locus_tag="KPE55_003687"
     CDS             269..445
                     /locus_tag="KPE55_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494674.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          422 a          137 c          102 g          429 t
ORIGIN      
        1 gcatataact ttatttatct tgaatcttat ttttaaaaac aattataatt ttagaatatt
       61 ttctattctc aaacaagcat gttgaataat ggagcatctt ctagtattat atacttgtag
      121 aataaaacta aattggtaaa actgggaata tttaacctta aggagttaag ttatagaaac
      181 tagtactaaa aaattttata aattatgtta attgactaat agaatgcgac tttttatttt
      241 tactaaattt tttaggggga tggataatat gtttattgga gaaatagaca aatgtacaca
      301 tatactaact gcttacatca gtagttcata tgattattgt aattttttag atacacagtt
      361 agatgatttt atatcagaat atggagaaac ggtagttgaa acctgcttac atcaagtctt
      421 attattagta agtagataca attaaaacta aatttttcta gaaaaacctt tttagattta
      481 tctactctaa taaatatata attttttgta ctttataagt aactataact tctgctaaag
      541 taaagatgta ttgggagtac atctttactt tcccattata gaagttaaaa gcatacacca
      601 taaaatcaat catccaatac aaattatata tttcctatgt ctaacttaat ttacaagaat
      661 cattctatat gcatattctg caatccacta aacatggctc taaaccttta ctattttata
      721 cattctctat aaatcataca aatcatatat aaaatccccc taatatgttt aaaccaccta
      781 taaaataaca ccaatattta gaatattcaa tatcaactgc ataactggtc aataaaaacg
      841 tataactaga caaatttcaa tatttatata aataatacaa gaaaaacaag cattttattt
      901 atataagttt tttatttttt gtttatatta aaaaactata ttgattaatt tattttattt
      961 agtaattata aaaattcgtc ttagaatata ttctatattt gtagattagc tttaatattt
     1021 ttaatttcac aaaaattaaa ctcccaatat ttttatccac ttttatctca ttaattaatt
     1081 atattatttt
//
LOCUS       DAELNY010000322         1069 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.325, whole genome shotgun sequence.
ACCESSION   DAELNY010000322 DAELNY010000000
VERSION     DAELNY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.325"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            135..887
                     /gene="cwlD"
                     /locus_tag="KPE55_003688"
     CDS             135..887
                     /gene="cwlD"
                     /locus_tag="KPE55_003688"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG0494675.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          474 a          110 c          164 g          321 t
ORIGIN      
        1 tatagtattg aatgttagat aaacatagtg aattaaatag gggcagaaat ataatatcat
       61 tacataataa tatattaata ccaataaata aagatatatc aatattaaaa aaatttaaat
      121 gtttgtacta atatatggaa tattatagtg tttttataag tatacatata cagggtgatg
      181 ttgtgagaaa gtacataaaa catataattt ttagctttgc aatgatatgt ctagtagtag
      241 tatccatatt tgaaattaaa aatatttctg aagatgttat caagtatatg ccagtaacaa
      301 acaaaactat aattttagat gctggtcatg gaggcattga tccaggtgca ttaaataagg
      361 ataagagtac atctgaaaag gatattaacc tagcaataac acttaagctt agagagctta
      421 tagaatcaag tggtggcctt gtaatattaa ctcgagaaga tgacagtagc ctttataaag
      481 aagaaaataa taaaactaca agacaaaaat ataatgagaa tttgaaaaat agaaaagaaa
      541 taatatctaa ttctaatgcc aatatgtttg tctctataca tcttaatgca tttgaacaat
      601 caaaatacta tggagctcaa actttttatc ctaaagacaa acaagacagt aaagaattat
      661 cgaagtgtat tcaagaagaa cttaaaagag tagtagataa gacaaacaac agagaagtca
      721 aaccaaggga tgatatatat cttttgaaag aaaataatat tccatcagta ctgatagaat
      781 gtggtttttt atcaaatgaa aaagagtgta aactcttaac tgatgaaaca tatcaagaaa
      841 aaatagcatg ggcaatctac ataggaatac aaaaatattt aagttgaaat aactataaaa
      901 ataggtctaa atatgtaaat agcttgtatt aaagcagtta aaatgcatta atataggcta
      961 tttttatttt gacaaaaaaa tatttaaaat aaaagttaaa aagttgttga cttagaataa
     1021 tatagatgat attatatatg agtgcccaaa aggagcacca aaataagac
//
LOCUS       DAELNY010000323         1062 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.326, whole genome shotgun sequence.
ACCESSION   DAELNY010000323 DAELNY010000000
VERSION     DAELNY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.326"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(179..394)
                     /locus_tag="KPE55_003689"
     CDS             complement(179..394)
                     /locus_tag="KPE55_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494676.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(577..921)
                     /locus_tag="KPE55_003690"
     CDS             complement(577..921)
                     /locus_tag="KPE55_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494677.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
BASE COUNT          350 a          158 c          127 g          427 t
ORIGIN      
        1 caatatacat tttttataat ttattaaaat aaaattcata tattaagaaa tattaatagt
       61 aaataagcaa ttgttactat aacataggaa ttatctttaa aaacacaaat taatttacat
      121 tctctaattt taaagtatat gtatatcttt gattttaata aaattcactt aaaatatttt
      181 aaggttgaac aatgaaattg aatgcccaag aacttcctcc tcttactaat gttgcaatta
      241 attcaaaccc acaattgtat tgaactgaat ctctacctac tggtttccaa ctagaaggtg
      301 cattatatga gaaagaattt gatgttggct ctttcttttg ttgctgctta attcctagct
      361 cattagaaat aaatccagtt tgatttatat gcacccttct atttgttaca cgaatctggc
      421 cgtctgcaat agaccagttt ccagaaactt tactaacgga aatatattga aatgctccat
      481 ctccttttga tattgtatat gtaactgtac cagatgcttt tacaccacca gttttatccc
      541 atccatcttc tgatatactt gcttttctaa gaggctttat tgtattagta tcaaaaaaac
      601 tgtcttagaa tatatctttg atgttaaatt tttagattgt ttaacttcta ataattgagt
      661 tgttgatata gaatcaacgt atatggactc tcctgttctt gcatttttga ttataccttt
      721 ttcattggaa cttttaacat cagtaattcc atttttagcc atatcgtata atttgtccat
      781 gtctgttact tgtttctctg aatacattac tttcatagat gaatcttcta ccttactagc
      841 acttgcattt gttggtaata gtattgtcat tatgagtaaa gataaaatag ttgttacaga
      901 tttttttaac ctataattca tattaatcct tactttttat ttgttataac agacattcta
      961 ttgtaataga atataaatcc acaatatttt taaaattagt attgaataac tattctcatg
     1021 ttataattat tcaaaggagg gattgatatg aggaaaaaat tt
//
LOCUS       DAELNY010000324         1015 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.327, whole genome shotgun sequence.
ACCESSION   DAELNY010000324 DAELNY010000000
VERSION     DAELNY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.327"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            36..791
                     /locus_tag="KPE55_003691"
     CDS             36..791
                     /locus_tag="KPE55_003691"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0494678.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVAEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKSNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
BASE COUNT          383 a          114 c          165 g          353 t
ORIGIN      
        1 ataacaaatt tttattaatt tcataggagg aatttatgtt gaaagttata attagtggct
       61 ataatggaac aatgggaaga gttttagcag aatgtgtaaa aaaagataat gaattagagt
      121 tggtttgtgg tgtagctagg tctgctgatg aaaaaggtca tcatagtgat acaaagcttt
      181 acaaaacaat gactgatgta gctgaaaaaa ctgatgtaat tatagacttt tcacatcatg
      241 agactttaga gccaatattg tcatatgctc taaaaaataa tacacctgta gtattagcta
      301 ctactggata tgatgaaaat gacttaaata aaatagaaga agcatcaaaa aacattccta
      361 tattattatc atcaaataca tctcttggag tgaatatatt aataaaatta gttaaggatg
      421 cttcaaagat gttagaaggt tttgatattg aaatcataga aaaacatcac aatagaaaag
      481 aagatgcccc tagtggaact gctactatga tagctaatgc aattagagaa gtcttgcctg
      541 aaagtgaaaa tacacatggt agatatggta gagattgtaa aagaaaatca aatgaaattg
      601 gaatacactc tataagaggt ggcaatataa tcagcgacca tgatgttgta tttgcaggtg
      661 ataatgaagt tcttacatta agccatcatt ctcaatctga tgaagtattt gcaaacggtt
      721 ctatagttgc agctaaattt ttaattggta agcaatctgg gctttataaa atgtcagata
      781 tattaggata agttttgtac ataataaatc tcaatctatt taggtattca tattcatcaa
      841 tttgaatatc taattgattg agatttctat tctaaaagtt tataaatttt atgtttacct
      901 tttaaatata atcatttttc ttaaagatta tcttatttat tggtatatgg aattatattg
      961 tttattattt tataatctct tcatcatata aacctattaa atattatcct attta
//
LOCUS       DAELNY010000325         1007 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.328, whole genome shotgun sequence.
ACCESSION   DAELNY010000325 DAELNY010000000
VERSION     DAELNY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.328"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   23..582
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=90..118
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
BASE COUNT          411 a          142 c           97 g          357 t
ORIGIN      
        1 acattaaaag aaaattataa aaacttacat tctgtatagt taatataaaa cataagtaat
       61 ataaatttat gtaaagcaac aaaagatgga tttacattcc atatagttaa tataaaacga
      121 tttcaaatga ttagaataaa aatatcagaa ttgctattta tattccatat agttaatata
      181 aaactaagaa tggaaaagga aataggagtt catattccaa catttacatt ccatatagtt
      241 aatataaaac tatgtatccc ccattaaaaa ggcttagatt aatttctatt tacattccat
      301 atagttaata taaaactcaa acatatctaa gttgtatttc tcaccttgta ggtcatttac
      361 attccatata gttaatataa aacggtttgg ttacaaggta tagtagaagg atatagtgga
      421 atttacattc catatagtta atataaaaca tggaactaag agagtacacc atcacatgat
      481 actcaaattt acattccata tagttaatat aaaactaaag ctggaaaaga aacagctata
      541 atgagtaata ataatttaca ttccatatag ttaatataaa accccaaaat aaacttagta
      601 ttttcaatac ttacacgtat acaactctct taaatttgca gtgaacgagc aatagtgcaa
      661 ttgataacgc ttatcataca ctctcaacgc cttatatttc aactgttacg ccaaattttt
      721 tcacaaatat cgctcactgc aaaattccta taattttatt atatcataaa tatattactt
      781 ttgaatattg atactaattt ataccataat aaaagcaaaa aaacgtaatc tatctacatt
      841 agatttaaat tctataactt aaagtttttt acataatatt tatgcttgtt taatcaatct
      901 aatatatcat tcttgtatat tgcttttaat agtctttttt cttaaataaa tatctatctg
      961 aacaagcata gtttaattaa aaacttttgt attcccttaa aactgta
//
LOCUS       DAELNY010000326          999 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.329, whole genome shotgun sequence.
ACCESSION   DAELNY010000326 DAELNY010000000
VERSION     DAELNY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.329"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            138..848
                     /gene="rgaR"
                     /locus_tag="KPE55_003692"
     CDS             138..848
                     /gene="rgaR"
                     /locus_tag="KPE55_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG0494679.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          405 a           83 c          172 g          339 t
ORIGIN      
        1 tattaaagag aatttctata agtagtttaa tcgacaattt aaatttataa agaaatttaa
       61 atttgaaagc tagtgagtac agtttggaaa ttgaaataaa gtaaactaaa tgatatttta
      121 gatttggagg tgatattatg ttcagggtag ttatatgtga tgacgaaaaa atacaaagaa
      181 gcatactaaa agagtttaca gaaaaacttt tgtctgaaag atgtctaaat tatcaaatac
      241 tggaattttc ctgtggagaa gatttaatat ccaaataccc agaaaaaatt gatattatat
      301 ttttagatat ccaaatgaaa gatatcaatg gaattgaggt ggctaaaaaa ataagaaagt
      361 ttgatgaaaa agtagaaatt atatttacaa cggctttttc agagtatgcc ccccaaggtt
      421 atgaggttcg tgcttataga tatttagtaa agcctataga gtatagtaac tttgcaatgg
      481 gtgtaaatct ttgtattgat aaccttcaaa gaaagaagga gcgttatata gttttaaata
      541 gtaaaaaagg atttaataga atactgatag attctatttt atatgttgaa actgtaaaga
      601 gagatttagt cgtacataca ataggtagag attataaagc taatatgagc atgaaacaag
      661 cagaaaaatt actgtgtgaa aatggctttt ttagatgtca tacaagtttt attgttaatt
      721 taagaagaat agaagatctt agggataata tgattactat taatgaacaa tttataccta
      781 taagtaagta tagacttaaa gatttaaaag tagcattaac aagcttgtta tgtgatataa
      841 tgtattaatt ttaaaaaggt aattgaaaat atcgtaaaaa agatttaatg tcatcacagg
      901 gctattgatt gaaactgtct taatatataa aatgttattt atattaagac agttttatta
      961 tgcaaaatag cagtttatag atttttaata tggtttaac
//
LOCUS       DAELNY010000327          997 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.330, whole genome shotgun sequence.
ACCESSION   DAELNY010000327 DAELNY010000000
VERSION     DAELNY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.330"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(231..965)
                     /locus_tag="KPE55_003693"
     CDS             complement(231..965)
                     /locus_tag="KPE55_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494680.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT          314 a          159 c           86 g          438 t
ORIGIN      
        1 gcatcttcta attctatcat tttaaatact ccccaatata ttttttattt aagatatttc
       61 tatgtaatat attatagatt ataaaagttg gaaatacttt tatcttatat ttaattataa
      121 ctaatagtta tctataagtg cagttcaaat tttataaatc taataaaaaa actgttccca
      181 gctagaaatt tccttctatc ttgaaacagc atcatatgct ttagaatatt ttattctaat
      241 aaagttcctt ttattatttt tacaatttta tcactttcaa tagtttttat agattttatt
      301 ttgttatcca caatacttat tgtaagtacc tcttttttta atattttgtt atcttctttc
      361 aatctataac tcttaactac tttactgcct tcatgattat atgctgttaa atcataattc
      421 gcatctccta caatttcatt tattctggaa tcttcaacac caacatactc tcccaggtca
      481 atatgtctaa aattttctag tgatacagcc gatttttcat ggtcactttt ttcaatcact
      541 atactagatt cagcatcaat gactgatata ccttgtgatg agaaagaatt ggtttcaaca
      601 ttgacaactt tattttcgtt aatatcaaca tataaagcac ttccatctag ttcatcattt
      661 ccagtatatt ttggataaac taatgtcata taacttgatg tatttatatc ttgcattgta
      721 tttgattctt ttaaaacttt aggttttata taataaaatg tattataagg tatacccata
      781 ttctttataa tgtacttata atctttattc ataatttcat taacatcatt ttgaactttt
      841 gtcatagttt tttgctgtgt tgctttactt gctacttgat ttttctcttt agtattttct
      901 ttatgacatc caacaagtat aatacctaaa atagttacac aaactaatga cctttttaac
      961 ttcataaatc aatcctcctt tcttaaattt tatagta
//
LOCUS       DAELNY010000328          988 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.331, whole genome shotgun sequence.
ACCESSION   DAELNY010000328 DAELNY010000000
VERSION     DAELNY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.331"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>988)
                     /locus_tag="KPE55_003694"
     rRNA            complement(<1..>988)
                     /locus_tag="KPE55_003694"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          220 a          291 c          223 g          254 t
ORIGIN      
        1 ctattagtat cgataagcta aatacattgc tgcacttaca ccttcgacct atcaaccaga
       61 tagtctttct ggggtcttac ccttgcggtg ggaaatctta tcttgaagtt ggcttcgcgc
      121 ttagatgctt tcagcgctta tccattccgt acatagctac ccagccatgc ccttggcagg
      181 acaactggta caccagaggt acgtccatcc cggtcctctc gtactaagga caggtcttct
      241 caaatttcct acgcctgcga cggataggga ccgaactgtc tcacgacgtt ctgaacccag
      301 ctcgcgtacc actttaatgg gcgaacagcc caacccttgg gacctactac agccccagga
      361 tgtgatgagc cgacatcgag gtgccaaacc tccccgtcga tgtggactct tgggggagat
      421 aagcctgtta tccccagggt agcttttatc cgttgagcga tggcccttcc acgcagaacc
      481 accggatcac taagtccgat tttcatcctt gctcgacctg tatgtcttgc aatcaagctc
      541 tcttttgcct ttacactcta cgtacgattt ccgaccgtac tgagagaacc tttgagcgcc
      601 tccgttacct tttgggaggc gaccgcccca gtcaaactgc ccacctgaca gtgtcccaag
      661 accagattca tggcctatgg ttagagtccc agtactacaa gggtggtatc ccaaggatgg
      721 ctccgccaaa actggcgtcc tggtttcaaa gcctcccacc tatcctgtac atgtagtacc
      781 aagacccaat gtcaagctac agtaaagctc catggggtct ttccgtcctg tcgcaggtat
      841 ccggcatctt caccggaatt acaatttcac cgagtctgtt gttgagacag tgcccaaatc
      901 gttacgcctt tcgtgcgggt cggaacttac ccgacaagga atttcgctac cttaggaccg
      961 ttatagttac ggccgccgtt tactgggg
//
LOCUS       DAELNY010000329          951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.332, whole genome shotgun sequence.
ACCESSION   DAELNY010000329 DAELNY010000000
VERSION     DAELNY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.332"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            291..713
                     /locus_tag="KPE55_003695"
     CDS             291..713
                     /locus_tag="KPE55_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG0494681.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT          419 a           88 c          111 g          333 t
ORIGIN      
        1 taattctttg tttaaatttt aatgcaaaat actttataaa tttatgattg aaaaacttgc
       61 tcaatcatat ataatagtac gtatctactc aacaataaaa attataataa actgtgtttt
      121 taaccctttc tattttaaga ttttattttt atatgatagt atacatactt atataggtaa
      181 gattttagct atataaatag ttttaacatg atttaatatg gcatattata ctattttaac
      241 ttaaaaatta acttataaat attaaattta tatacagaag ggaaaacgat atgaatacat
      301 ttaaaactga agttttatta aaatattctg caaaagacat atttaatata tttacaaaat
      361 ctgcaagact aaattttcct aattttaatg agaaaaaagc agtaggaaca tttgtacagc
      421 aaaaaaataa taaaagattt aaggttgaaa taactaattt cgaaaaaaat agagtatatg
      481 agataaaaac aagcaataaa agagaaagta ctattactag atatgaatta gtacatattg
      541 acttagaaaa tacaaaactt atttttattg aaagtgagag caagaggaat atatttggaa
      601 aaatcaattc agctcttgta agagtattgt ttggaaaaag gcaaccagaa gaatttaata
      661 aatttataaa aaaactagaa atggaattgg agaataatag tgttaattta tagatataag
      721 ttagattata tatttaaaat ctatctaaaa acatatttct aagtatttta tgagtcctac
      781 tattcgttac aataattaat gaaaaaacta ttgataaaca tgtgacacta tgactatata
      841 ccacattaag agtatcatta gcaataataa ttttatgtat tattgctttt agttaaaaaa
      901 agagagaaaa attaataatc tctaatatcc cttaaacttt caataaagag g
//
LOCUS       DAELNY010000330          933 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.333, whole genome shotgun sequence.
ACCESSION   DAELNY010000330 DAELNY010000000
VERSION     DAELNY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.333"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            51..>933
                     /locus_tag="KPE55_003696"
     CDS             51..>933
                     /locus_tag="KPE55_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG0494682.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGY"
BASE COUNT          383 a           80 c          147 g          323 t
ORIGIN      
        1 aggattataa aaaattttta gaatgtaaat tgaaatttag gagtagatgt atgtatatac
       61 tgaatttaaa tacaagagag actattgaag attttagaga taaattttat gtagcagaaa
      121 attcttatct tatattaagt gctccaaaaa atttaaaatt gcttaaggaa actttggata
      181 tagatgaaat aacttttaat gattgtctta aatttgatga aataaccaaa cttgatttat
      241 ttgataatta cgatttttta agcctaaata catttgaact aagagatgga gaagctgtta
      301 tagaagaggt aaatatgtat ttatcagata attttatatt ggttgtagtg aatgaagaac
      361 actttttatt tgaatttgta aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc
      421 cagttattaa tctatttaag ataaactact tgattcttag agaagttata aaaaatggtt
      481 ttgagtcttt ggaaaaagtt gaagagttaa ttcttcaaat agaagatgaa atgatggaca
      541 acattaataa gaatcatgta tctagaataa gtgatgtaag aggattaaca agaattatag
      601 ttaagaatac tagacctctt ttgtatattg gggatagaat agttaaggaa aatataagat
      661 atttaaaata ttcaaatgta aaaaaatata atttagaaaa ttttcaaggt atagattttg
      721 gaattgacaa attatacaat ttcgcattgt caacacgaga gttagcagat aaattattag
      781 atatttattc ttccagagta ggtgagaaaa caaataactt aattacaaaa ttaaccttgt
      841 taacagccat atcagcacca cttacaatta taactggtat atatggaatg aactttagat
      901 atatgccaga attaaactgg atttatggat atc
//
LOCUS       DAELNY010000331          904 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.334, whole genome shotgun sequence.
ACCESSION   DAELNY010000331 DAELNY010000000
VERSION     DAELNY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.334"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(62..553)
                     /locus_tag="KPE55_003697"
     CDS             complement(62..553)
                     /locus_tag="KPE55_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG0494683.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          335 a          143 c           80 g          346 t
ORIGIN      
        1 atcaaaaggc ttcatatcta agatatgtga tgacactaat attcacaatt tttagtaatt
       61 atcacattat aaaaaatatt acagctagtc catcattaga tgcttcttgt ataaactttt
      121 taagctcaat tagatttgcc catacatatt caaaaaaatc atcttcactc tcatcattca
      181 taacaggata aatattattt tctaacatct cattaatatt aaatcttttt ctaaatgctt
      241 gttcttccaa actgttcatt gcttttgata gtttttttac atcatttgct gtaatcaaca
      301 tgacaggaaa ttctccatca atttccataa gtatgtttcc acttagtact aatttgctaa
      361 atatattatc atcatctcca ccaaaaggac aacctgtcaa agtaaagtga atggcatgcc
      421 atgctttatc aatattgata gtactatttt cgttggcatc ttcaaaaaca aaatcttgta
      481 aattttcttc tgcttgctgt aatttaaaaa cattctcttc acttatttta gtataacatc
      541 ctatcatccc cataattaca ttcctcctaa gtagcgtttt tttaattgta acttataatt
      601 aaacttagtt acaataaaat atattgagaa aagtattatt tcatcttaaa tttaaatacc
      661 tatattatca actaaaattc tgtgtttata gcgtaagtat tattattttt tcctaaaaat
      721 caaatgaatc agaagttcta taggcaatat tgactataaa taagaactat tacttttaaa
      781 aaaaatttaa gaaattatac tgtagaatca tgctctactt attagcttca aataaaaaat
      841 aaatcatgtt ctcaaattaa ataataaata acaataaatt taaattttaa actataagag
      901 tttc
//
LOCUS       DAELNY010000332          888 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.335, whole genome shotgun sequence.
ACCESSION   DAELNY010000332 DAELNY010000000
VERSION     DAELNY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.335"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(91..801)
                     /locus_tag="KPE55_003698"
     CDS             complement(91..801)
                     /locus_tag="KPE55_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494684.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          297 a          124 c          103 g          364 t
ORIGIN      
        1 aaaattaaat ttgaaaaaac aaaactctaa aaactaagct ttaaaaaatt aagtttcaaa
       61 aataaaattc tagagattga acttcaaaaa ttaagattct aacatatacc ttgtaaattt
      121 tcctacaacc tcttgatatt ttttacggct tataggaatt ttttcattct gaactttggc
      181 ataagtttga gttatactat ctacatggtt tagattgaca atataccttt gatgcgtacg
      241 tataaaatta gagggaagaa cagtttccat atcatttaat tttccgtagt attctaatgt
      301 tttatcaaag gtaactacag ttaattttct tagctcactt ttaaagtaga ggatatcttt
      361 tgtattaatt ttacaaagat tagttccaat ctgaactgta ataaagtttt ctttccattg
      421 ttctaatttt tttagtgctt cttttaatac tttacatatt ttttcagttt tataaggttt
      481 taagatgtag ttcaatgctc ctacatcata tccatgaaat acatagtcag aaaaacctgt
      541 gacaaagatg attatagtat cctttgaatg ttttcgtata gtttttgcta tgtcaacgcc
      601 attacttttt ccaagttcaa tatctaaaaa aataatatct atgtaataat tttcgtgttc
      661 tagattgtat actagacttt ctccatttga atattctttt atttggtagg ggtagcccca
      721 caaatccatt tctcttgtaa tgattttatt cagcatgttt ctttgttgtt tttcatcatc
      781 acaaatacaa atcgaagtca attttttatc tcctttcaaa ttttatattt atatagttaa
      841 gtatataata tattttgcta cctagatata taaaatgaaa taacaatt
//
LOCUS       DAELNY010000333          851 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.336, whole genome shotgun sequence.
ACCESSION   DAELNY010000333 DAELNY010000000
VERSION     DAELNY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.336"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            69..770
                     /locus_tag="KPE55_003699"
     CDS             69..770
                     /locus_tag="KPE55_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG0494685.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
BASE COUNT          338 a           77 c          146 g          290 t
ORIGIN      
        1 gtattaaaaa tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa aaagaatagg
       61 agataaatat gagtattgaa gatttatatg attttgaatg tgttcctttg aacaatgaga
      121 ttgatattga tgttttattt gaaaagatga tagaagatgg aaaagaaaaa gaatatacac
      181 caattatagt cattgatgaa aaagtagggc tattaagaaa aaatatagat atgattacta
      241 aagagtatgg ttctcttgaa aactatagag agtattgttt aacaaaatat aatgaaattg
      301 atgtaaatca attttttgat tttaggaaga gtgagatttc agaattatta aaggagctaa
      361 ttgatttaga agatgatttt tatgattata aacattttag acccattatg aatatagata
      421 tatttcatga atataacaaa gtttacatag ctaaaattcc aacaattaaa ccatatgaag
      481 tatttgcata catacctatt ggcggattta atgattgtcc tagagatgaa gaacatatag
      541 ctattgcaaa atattggtat gaaaagcatg gaatctttcc aatcgcaatt ggatgtgata
      601 cagttcaata ttctgtaaaa aatttaacta aagatggtaa aaaatttgat gacttatgta
      661 ttgaattagt attttattgt gaggatatta ttatacaagg gtatgaaacc ttaaaggctc
      721 ttaaggatgt attaaaacgc tctacaatag tgcttttttg gtgggattga tattctatca
      781 tttagatagt attaaattgt tagtcaccaa gaatccactt gctttagtta tagggagtgt
      841 gtcaaactag g
//
LOCUS       DAELNY010000334          846 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.337, whole genome shotgun sequence.
ACCESSION   DAELNY010000334 DAELNY010000000
VERSION     DAELNY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.337"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            133..630
                     /locus_tag="KPE55_003700"
     CDS             133..630
                     /locus_tag="KPE55_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0494686.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT          343 a           59 c          152 g          292 t
ORIGIN      
        1 gcatttagta tataatatct cactgagttt aattatatta aaaatatgac ataaaggtgt
       61 cgtatattat aaattataat tgaattagtt caaaataaaa tagattaata aaaacaaagt
      121 gaggtataaa gtatgaatta tgaaatagta gagataaatg agaaaatagt tataggaata
      181 tcgaaggaga ctacaaataa agatggacaa gcggtaaatg atataggtga attgtggaaa
      241 aaatttatgg gcaaagggat atataatgct ataaagggta aaaagaatga caagacaata
      301 ggtctatata cagattacca gggtgatttt acaagtccgt atagctttgt tgcatgttgt
      361 gaagtaaata gtaattctga taaagagaag aatttagaag aattaaacac taataatact
      421 gttggagaaa gtattattag taaagttata ccagcaggta agtatgcaaa gtttgttata
      481 gtaggaggtc aaaaagaagt aggagatttt tggtttgagt tttggcaaat ggattttgat
      541 aggacttata taagtgattt tgaagagtat caatgtaata cttttgatac aaagaaacaa
      601 gaaattcata tttacatagg aataaagtag atttgttatt actatattga atgaaatatt
      661 gagtatagta catttattat agatagtttt attatttata actataagca gtaaatctgt
      721 aaaagattta ctgctttttt agtaattaat agtatcagta tcactttgaa tcttcagaca
      781 tattcttcat ttaatattgt ttattgataa atgatacttg taaatataat gatttgttat
      841 ttaatt
//
LOCUS       DAELNY010000335          804 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.338, whole genome shotgun sequence.
ACCESSION   DAELNY010000335 DAELNY010000000
VERSION     DAELNY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.338"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            94..660
                     /locus_tag="KPE55_003701"
     CDS             94..660
                     /locus_tag="KPE55_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG0494687.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          310 a           67 c          150 g          277 t
ORIGIN      
        1 atttaataaa tataaattaa attatatgtg tatatattgt actttaatag tgatataata
       61 ataaaaaaat ttttaaagag ggtgatatta catatgagaa aacttgaaga gatattagaa
      121 tataataaat catttgtaga gaacaaagaa tatgaacaat atgtgacatc aaaacatcca
      181 aataaaaaaa tagtagtctt atcttgtatg gatacaagat taactgagct tttgcctaag
      241 gctatgaatt tgaaaaatgg agatgtgaaa ctaataaaaa atgcaggagc tactataatg
      301 catccttttg gaagtattat gagaagtgtt ttagttgcaa tttacgaatt tgatgtagat
      361 gaggttatgg ttgtagggca tcatggatgt ggtatgtgta atgttgatac ggataagtta
      421 cttggtaaaa ttttagatag aggaatatct aatgatgtaa tacttacact tcgtagtgct
      481 ggaattgata caaaacaatg gttacatgga ttcgattctg cagaagaatc tgtaaaagat
      541 agtattgatt taataaaagg gcatccttta attccagatg gtattattgt acatggtcta
      601 attatatcac ctgaaactgg gaagttagac gttgttgtaa atggatatga agataagtag
      661 agtatcataa tgttatataa tcaagatact gttgaatatg atgttgtttc aaaatttatt
      721 ttgggacaac ttttttattt gaaaatatat gaaaaggttt attttaagct ttgcaataaa
      781 aaatataagt agctgactaa ttaa
//
LOCUS       DAELNY010000336          753 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.339, whole genome shotgun sequence.
ACCESSION   DAELNY010000336 DAELNY010000000
VERSION     DAELNY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.339"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>753
                     /locus_tag="KPE55_003702"
     rRNA            <1..>753
                     /locus_tag="KPE55_003702"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAELNY010000337          716 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.340, whole genome shotgun sequence.
ACCESSION   DAELNY010000337 DAELNY010000000
VERSION     DAELNY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.340"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..634)
                     /locus_tag="KPE55_003703"
     rRNA            complement(<1..634)
                     /locus_tag="KPE55_003703"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          173 a          182 c          127 g          234 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccagttaa aaaggtttga tagattagaa
      661 gctatcaact tcgataattt taaaaagtta tcagctttat atattacaaa ttagat
//
LOCUS       DAELNY010000338          673 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.341, whole genome shotgun sequence.
ACCESSION   DAELNY010000338 DAELNY010000000
VERSION     DAELNY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.341"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            162..530
                     /locus_tag="KPE55_003704"
     CDS             162..530
                     /locus_tag="KPE55_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0494688.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          269 a           70 c           91 g          243 t
ORIGIN      
        1 tcccctcttc cataaaactc tcaatatctt ttttatctat aaatctatac tacttagttc
       61 tcaagcataa aataatataa agattattta tacatgatga tatattatat tgactattag
      121 acatttgtct aatataatca agttaaaggt gagtgattat attgaaaaaa ataccaaaat
      181 ccgagttgtt agttatgaat ttcatatggg ataaaggcat agataaaata ccagccagag
      241 aagtagctaa ttatatggaa atttctcatg gatggacaaa aggtactact ggtaaagtac
      301 tttctagatt aagtgataaa ggttttttag aatttgaaaa agatggtaga aatactttat
      361 acactattaa agttaaacat gaggaatatc taaaatttga aacaaaggat tttttagagc
      421 ttatacataa aaattctatt catagcttta tatcatcatt aggttgtgga aatgaaatat
      481 cagatgatga cataaaagaa cttgaacaat ggctaaaaaa aagaaaatag gagacgtatt
      541 ttatggaatt atttatagaa gttattaaat ttaatattta cataagtcca ctaattcttt
      601 ttttcatttt atttactaaa aagataagca aatatacttt taagtttaat tattttatat
      661 gtcttttaat agt
//
LOCUS       DAELNY010000339          664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.342, whole genome shotgun sequence.
ACCESSION   DAELNY010000339 DAELNY010000000
VERSION     DAELNY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.342"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>664)
                     /locus_tag="KPE55_003705"
     rRNA            complement(<1..>664)
                     /locus_tag="KPE55_003705"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAELNY010000340          643 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.343, whole genome shotgun sequence.
ACCESSION   DAELNY010000340 DAELNY010000000
VERSION     DAELNY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.343"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          208 a           79 c           70 g          286 t
ORIGIN      
        1 ttttttcatc acattttcgc ttgtcaagaa taatatcttt aagagtgaag cgtttgatgt
       61 aaatctggac tttgtttttg agacattttt gaaatattta ttaaatcttt cgattatact
      121 tgtaatatat attatagata ttacttattt tagtaaaact aaaggatgat gctacaacat
      181 tccagttatt tcccaaaatt ttaatttctg aagtatactg tttacattat ttttaatcta
      241 ttttatctaa agtcgataag gcttcttatt cattaactga cctatataca gatttaaaat
      301 tacttattaa ttcttttgag tccttgtatg aaacatattt aaagtatttc ttatctaatg
      361 tattaaacat ttttgtatta cagaatgagg ataacttgta gctattgtct ctttgaaata
      421 agtcatctgt gtttactcct aaaactactt atatctgtag attttataag ttctccatct
      481 tatataattt ctttgattaa ttgttttctt aaattattca taaaaaaatc tccgttacaa
      541 tatttgatta tatttaaatc attgctataa agaagacttt tattaacttt gaaaaacaaa
      601 ttattttaat tgctggtgat tagttgctac cttcttttga tat
//
LOCUS       DAELNY010000341          640 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.344, whole genome shotgun sequence.
ACCESSION   DAELNY010000341 DAELNY010000000
VERSION     DAELNY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.344"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            133..573
                     /locus_tag="KPE55_003706"
     CDS             133..573
                     /locus_tag="KPE55_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0494689.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
BASE COUNT          241 a           82 c           97 g          220 t
ORIGIN      
        1 aaaactaatt accttatatt tttacgtatt ggtataataa tacataaaaa cttgaataat
       61 gcataaaaac ttgacatact gttgtcataa ttactttgct ataattaaaa gtaaaaatgg
      121 aggtaaattc taatgtatga acgtatgctt gataaaagca ataaaccctg tttgtctcaa
      181 ataatagaat atataggtaa agatggatat gcacgattgt gtaagttgga aacttacttg
      241 aaaactcaat ataatttatc aagagaactt cgatttcctt ttggaaacaa ttatggttgg
      301 ggttacaaat atagccataa atcttttcat ttattttatg ctttttttga aaaaggtgca
      361 tttactgttc tgattcagat aggtgacaag caagttccag cagtagaaaa aacgttggta
      421 gctttgacac caaaagctaa agaactctgg gtcactcgtt atccttgtgg caataatgga
      481 ggctggatta agtatcgtat tttagaagat gcagacttac ttgatattat tcaatatata
      541 agagcaaaaa aatctccacc taaacaaaat taacttttat tttttgtgat ttaaatatag
      601 tgtatataaa ttccattctt tccatataaa aaaaataata
//
LOCUS       DAELNY010000342          636 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.345, whole genome shotgun sequence.
ACCESSION   DAELNY010000342 DAELNY010000000
VERSION     DAELNY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.345"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          263 a           38 c           89 g          246 t
ORIGIN      
        1 tattaggatt tatatatatt cagatttatt attatattga gccttatatt aaattaagga
       61 ctataatttg aaaataagct ataaaaactt aatattgttt tgttcagaga taaaaaaaga
      121 atactttagg tgatttttat taaaaattaa gagtgataaa attatatact ttataaatgt
      181 aaatatgacg aataaaaatg ataaatataa taatatatat atttacatta ggagttttct
      241 atactataat tatttaaaac taaatataaa tgttggaatt atttaggact tgaaattgtc
      301 taaaaataaa tacaagtgat gaagaggtga tgtgatgaga ttagatatgg atagtatgtt
      361 atatgtacta atttatatga cattattggt atctatcatt ggaagtatat tttatttttg
      421 taaagacctc ttaaatcata aaattaagta tgttttaata agagacatat ttgtaaatag
      481 aattacatat tctgaagaac gactgcctaa acagtatata gtttttcaga aatatgatat
      541 ttggaggtat tgtagtttgt tttaatttga aatgaatcta ttataaattc taaagtttaa
      601 aaataaaaag gagagaataa aatggatttc atttca
//
LOCUS       DAELNY010000343          625 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.346, whole genome shotgun sequence.
ACCESSION   DAELNY010000343 DAELNY010000000
VERSION     DAELNY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.346"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..338)
                     /locus_tag="KPE55_003707"
     CDS             complement(<1..338)
                     /locus_tag="KPE55_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0494690.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKA"
     gene            complement(469..>625)
                     /locus_tag="KPE55_003708"
     CDS             complement(469..>625)
                     /locus_tag="KPE55_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3785 family protein"
                     /protein_id="HBG0494691.1"
                     /translation="EYVISNISVDYKGLSFNKLSRANKVDNSYIVSVIICENCQDYIV
                     QIENCIV"
BASE COUNT          227 a           98 c           45 g          255 t
ORIGIN      
        1 gccttattta catcttcatt ttttaaactt aaaatagcta tttgtgaata aaatcgtgct
       61 aaaaatttat aatctttggg taagcattta tcaatatatt tacaggaaat caaaatatac
      121 tctttcattt tttcactaaa cccttgaata ccatataata aagctagcaa aaaatttgat
      181 tttaataaca ttaaactatt ttgttcagtt ttatagcaat caattaaatt ctttactaca
      241 tcaattttat tttcaataaa atcgttatta gtcaataatc tttcattcaa ggttagttta
      301 aacattgaat ctaaaattgt atcatctatc tcttccaaat atttactata tattgtaact
      361 cacctccgac ttacattatc tcataaattt agtaattttc catactattt ttgaaaaaag
      421 ttgatgtctc aatttagaaa tttctcttaa attaagacat actttacact aaactataca
      481 attttctatc tgaactatat aatcctgaca attttcgcaa attatcacac ttacaatata
      541 actattatca actttatttg cccttgatag tttattaaaa gatagtcctt tataatcaac
      601 agatatattg cttattacat attct
//
LOCUS       DAELNY010000344          577 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.348, whole genome shotgun sequence.
ACCESSION   DAELNY010000344 DAELNY010000000
VERSION     DAELNY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.348"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>577
                     /locus_tag="KPE55_003709"
     rRNA            <1..>577
                     /locus_tag="KPE55_003709"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAELNY010000345          550 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.349, whole genome shotgun sequence.
ACCESSION   DAELNY010000345 DAELNY010000000
VERSION     DAELNY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.349"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            243..>550
                     /gene="tcdR"
                     /locus_tag="KPE55_003710"
     CDS             243..>550
                     /gene="tcdR"
                     /locus_tag="KPE55_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG0494692.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     "
BASE COUNT          224 a           40 c           65 g          221 t
ORIGIN      
        1 tttactttaa taaaatgatt tgtttttaca atactttatt aatataaagt ttattgctaa
       61 aatactttat ttattagaaa aagattacta attaattatt aaaattaatg tattcataat
      121 gcatattttc atataaaatt taatttattt gccgattata taattataat gactgattta
      181 attccaatgt tgtcaaaatt ttcgaataaa tcatcattat aaatataaga gaggatgatt
      241 ttatgcaaaa gtctttttat gaattaattg ttttagcaag aaataactca gtagatgatt
      301 tgcaagaaat tttatttatg tttaagccat tagtaaaaaa acttagtaga gttttacatt
      361 atgaagaggg agaaacagat ttaataatat tttttattga attaataaaa aatattaaat
      421 taagtagctt ttcagaaaaa agcgatgcta ttatagtcaa atatattcat aaatcattac
      481 tgaataagac ttttgagttg tctagaagat attctaaaat gaagtttaat tttgtagaat
      541 ttgatgaaaa
//
LOCUS       DAELNY010000346          531 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.350, whole genome shotgun sequence.
ACCESSION   DAELNY010000346 DAELNY010000000
VERSION     DAELNY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.350"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          234 a           48 c           57 g          192 t
ORIGIN      
        1 aatcagtatg attataacac aaattataaa ttcaaactac atattttata gttatcaatt
       61 taatatttaa ctttataaga gctatctcaa aatagagata aggctctttt tcattgttaa
      121 aatgatgaaa acatatttaa tctaatacta aaatatattt aaattaatac aaagaagagt
      181 gcctaataag ctaaaaagtt cattttaaga caccttcttt ttataatata tattttagtt
      241 aacttgatgt tttttaaaat tatttttaat aaaaggtaaa aaaacaactg taagaactaa
      301 gcatgtaaaa ataatcattg taggtaaagc tattattaaa ttaccaaatt tataatctac
      361 ataattagta aatgaaaatg aaatagcatt tgatggtaaa acattgagaa tttgattata
      421 caacttattt gtttcactgt atttgaaaaa tattggtaca aataacaata ttatatttgt
      481 aattaaaact ggaaaaggag atttcatata agacgataat aaaagtgtaa a
//
LOCUS       DAELNY010000347          530 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.351, whole genome shotgun sequence.
ACCESSION   DAELNY010000347 DAELNY010000000
VERSION     DAELNY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.351"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..440
                     /locus_tag="KPE55_003711"
     CDS             <1..440
                     /locus_tag="KPE55_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG0494693.1"
                     /translation="FYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCR
                     AKNKLVNVVDNIEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYE
                     EYLNVLGDIRKKVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT          245 a           37 c           94 g          154 t
ORIGIN      
        1 aattttatga tttaaaagga aatataaaaa taataaaaga tgattataaa gaaatatata
       61 taaaagatgc ttctgtaata attgctgcca caaataatag agagttaaat atggaaatag
      121 gattatattg tagagcaaaa aataagctag tgaatgtagt tgataatata gaaatatcga
      181 actttactgt tccatcatat ataaaaagag gagatttact tataagtgtg tctactgggg
      241 gcaagagtcc ttcattatca tcaaagataa aaaaagaaat tgaagagaga tatactgaag
      301 attatgagga gtatttaaat gtattaggag atataagaaa aaaggttata aaaaaatacg
      361 aagataaaag taaaagaaaa gatgtgctta atatgttggt aacacttgac ctagaagaac
      421 ttaagaagtt tgatatataa aaaataaaat aaaaaatgtt aaaaaggtgt tgacggatta
      481 tttttaagat gatatagtat tacttgtcct taagaaaagg gcaaacataa
//
LOCUS       DAELNY010000348          503 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.352, whole genome shotgun sequence.
ACCESSION   DAELNY010000348 DAELNY010000000
VERSION     DAELNY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.352"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          233 a           55 c           37 g          178 t
ORIGIN      
        1 gaacaattat ttatatgcta taaaaatgct aatttgaacg aacttgtctt ataatcagtt
       61 ttagaaaata caccattcta caaagtatga atcaaaaggt gaaaaaatac taaaaataat
      121 aaatcaacat caattttaat caatatatga aaaacttata ccaagtatgc ataaaaacta
      181 atataaatat tattttaaca actaatactt acacctttat atattatatc atatttcaat
      241 aaataaattg cataaaagga aatcttttca tagaattaaa ctatttatta attcattact
      301 ataggttcaa taaactataa gatgtcgctt taatatttat attgtactcc attaaatatt
      361 aatataatta attaaagtat gcatagctaa atgaaacaaa tatctataga gttttataaa
      421 tagtcattat tttaaaaagg taatcatatt ctaaaaatac atataataca tataaaaatc
      481 aataatagta tattgataat caa
//
LOCUS       DAELNY010000349          482 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.353, whole genome shotgun sequence.
ACCESSION   DAELNY010000349 DAELNY010000000
VERSION     DAELNY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.353"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            31..>482
                     /locus_tag="KPE55_003712"
     CDS             31..>482
                     /locus_tag="KPE55_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0494694.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYG"
BASE COUNT          205 a           45 c           70 g          162 t
ORIGIN      
        1 ttagaagaaa ttctataagg ggggatattt atgcacaaga gattgctcac gttgtttaaa
       61 ttattaaatg aaagtgatga taaaattact tgtaaaactt taagtaatca tttaaaagtg
      121 agtgagagaa ctataagaaa tgatattact tctataaatg gaactttaga gaaaaatggt
      181 gctattatta aaataaaaaa aggagaagga tattatatag atattcttaa tttagcatta
      241 taccaacagt atctagcatt aatatctgat gatattatgg attcaagtga aataccagat
      301 tctcctatag aaagaaatca atatatttta aaatatattc tctataacaa tacatatata
      361 aaactagaag atttagctaa tagcttgtat gtgagtaaat ttacaatact aaatgacata
      421 aaaagaataa agcctatttt gtcaaagtat aatttaatat tagtttctaa gccatattat
      481 gg
//
LOCUS       DAELNY010000350          482 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.354, whole genome shotgun sequence.
ACCESSION   DAELNY010000350 DAELNY010000000
VERSION     DAELNY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.354"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            148..>482
                     /locus_tag="KPE55_003713"
     CDS             148..>482
                     /locus_tag="KPE55_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494695.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNTRRHNIL
                     TKLILSIIV"
BASE COUNT          240 a           37 c           59 g          146 t
ORIGIN      
        1 tttgaaaaat cttaaaaaaa gtaaaagtta tgataataaa aataatatta aagataataa
       61 gatatataag aaacttgaaa aatttgtaat ttttagttat atatatatta taatgaacta
      121 gttaaaaatt tttatgaggg ggaaaatatg aataagaaga aaaaattgat tgggatagct
      181 atatcttttc tacttataat cagcattata aatccattac aagtagaagc agaagctatt
      241 attgagtcta gtgtatacat ttcttcaata aaagagaatt atacatatga taatactaaa
      301 tttaaaacta ttaaatctaa aagtagacat aaaagtagga aatataataa aagtaaagta
      361 aagttgaaaa aacgaagtat aagaactaaa aagtatggat atcaaactat aaataaatat
      421 aaaagacaaa acacaagaag acataatatc ctaacaaaat taatattgtc cataatagtc
      481 at
//
LOCUS       DAELNY010000351          450 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.356, whole genome shotgun sequence.
ACCESSION   DAELNY010000351 DAELNY010000000
VERSION     DAELNY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.356"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>450)
                     /locus_tag="KPE55_003714"
     CDS             complement(<1..>450)
                     /locus_tag="KPE55_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494696.1"
                     /translation="DLYNEVKGEFSKINFHGFDYKNWQSSAKITENLKEKHFNLDREY
                     NRSYFLTLPDMYLVELQQSKILQFMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKK
                     LSKIGLSFKNMNKIVSIEIGTLFFIPYFIAIINSIISLLFLNQVLNNS"
BASE COUNT          166 a           65 c           37 g          182 t
ORIGIN      
        1 actattattt aatacttgat ttaaaaacag taaagaaatt atgctattta taattgctat
       61 aaaatacggt ataaaaaaca atgtacctat ctctatacta acaatcttat tcatattttt
      121 aaaagataga cctatttttg atagtttttt gtactttaca ttatcttcta tctcgtctgt
      181 atataatctg aagtataaaa aactaaaaac tgctataaat aatatcactc ctataaaaac
      241 acccataaat tgaagaattt tactttgttg taactctact aaatacatat ctggtaatgt
      301 taaaaaatat gacctgttat attctctatc caaattaaaa tgtttttctt ttaaattttc
      361 agttatcttt gcactgcttt gccagttttt ataatcaaat ccatgaaaat taatttttga
      421 aaattcacct tttacttcat tgtataagtc
//
LOCUS       DAELNY010000352          375 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.358, whole genome shotgun sequence.
ACCESSION   DAELNY010000352 DAELNY010000000
VERSION     DAELNY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.358"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            10..300
                     /locus_tag="KPE55_003715"
     CDS             10..300
                     /locus_tag="KPE55_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG0494697.1"
                     /translation="MYLNEIKYIEVQKKDMLIHTINKNFDIKYSLGKIEKELNPYKFI
                     RCHKSFIVNLRYVENIKPNTAILESGEEVPISRYRYKEVKEKFLKFLGDTIC"
BASE COUNT          169 a           30 c           52 g          124 t
ORIGIN      
        1 acatataaga tttatttaaa tgaaataaaa tatatagaag ttcaaaaaaa agatatgcta
       61 atacacacaa taaacaagaa ttttgatata aaatatagtt taggcaaaat agaaaaagaa
      121 ttaaatccat ataaatttat aagatgccat aaaagtttta tagtaaattt aagatatgtt
      181 gaaaatataa agcctaatac tgcaatactg gaaagtggag aagaggtacc tattagtaga
      241 tatagatata aagaagttaa ggagaaattc ttaaaatttc ttggtgatac aatatgctaa
      301 actttatgac tgagctatta gtagttttat ctatatttat acagttttct ataatgaacg
      361 gaattttaat ttata
//
LOCUS       DAELNY010000353          363 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.denovo.360, whole genome shotgun sequence.
ACCESSION   DAELNY010000353 DAELNY010000000
VERSION     DAELNY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.denovo.360"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="KPE55_003716"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="KPE55_003716"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="KPE55_003717"
     tRNA            290..361
                     /locus_tag="KPE55_003717"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAELNY010000354        10627 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.guided.127, whole genome shotgun sequence.
ACCESSION   DAELNY010000354 DAELNY010000000
VERSION     DAELNY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.guided.127"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..838
                     /locus_tag="KPE55_003718"
     CDS             <1..838
                     /locus_tag="KPE55_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0494698.1"
                     /translation="PPIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLE
                     AAWEFRLKGLEVVVVEAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDG
                     KAQKVVFKDGDSVDCDMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYAC
                     GDVAQVGNISLAIWPSSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQ
                     NFDKEISSKDEGQRIYKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKAD
                     IL"
     gene            1272..1973
                     /locus_tag="KPE55_003719"
     CDS             1272..1973
                     /locus_tag="KPE55_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0494699.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            2023..3105
                     /locus_tag="KPE55_003720"
     CDS             2023..3105
                     /locus_tag="KPE55_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0494700.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            3366..4466
                     /gene="vanG"
                     /locus_tag="KPE55_003721"
     CDS             3366..4466
                     /gene="vanG"
                     /locus_tag="KPE55_003721"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0494701.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            4463..5269
                     /locus_tag="KPE55_003722"
     CDS             4463..5269
                     /locus_tag="KPE55_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0494702.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            5287..7425
                     /gene="vanT"
                     /locus_tag="KPE55_003723"
     CDS             5287..7425
                     /gene="vanT"
                     /locus_tag="KPE55_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0494703.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            7739..8359
                     /locus_tag="KPE55_003724"
     CDS             7739..8359
                     /locus_tag="KPE55_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG0494704.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(8525..9061)
                     /locus_tag="KPE55_003725"
     CDS             complement(8525..9061)
                     /locus_tag="KPE55_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0494705.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(9174..9848)
                     /locus_tag="KPE55_003726"
     CDS             complement(9174..9848)
                     /locus_tag="KPE55_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG0494706.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLIATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            10051..>10627
                     /locus_tag="KPE55_003727"
     CDS             10051..>10627
                     /locus_tag="KPE55_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HBG0494707.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMI"
BASE COUNT         3984 a         1158 c         1779 g         3706 t
ORIGIN      
        1 accaccaatt aagggccatg atttagaaaa tgtgtttact cttagaaata taaaagattt
       61 atatagtgta aaagaagcac tagaaaaatc taagaaagtt gtagtgattg gtggcggttt
      121 attgggactt gaagctgcat gggaatttag actaaaagga ttagaagtag tagtagttga
      181 agccatggat agcatactat caaaacaact tgataaagaa ggtagtaaaa tattagaaca
      241 atgtgtaaga gatacaggaa tagatgtaag attgggtgta gcagttgatg gtattgaagg
      301 tgatggaaaa gctcaaaaag ttgtatttaa agatggagat agtgtcgact gtgatatggt
      361 agtattttca attggtgtaa gagcaaacac tcaaatggtt caggatacat ctgtaaaaat
      421 tgatagaggt attgttgtag ataagacatt acagacaaat gttaaagata tatatgcttg
      481 tggagatgtt gcacaagtgg gaaatattag tttagcaata tggccatctt cagtagaaat
      541 gggaaaaata gcaggggcaa atgcaagtgg agataatttg acatttgaaa gtgaagtata
      601 tccagtttca ttagatgcaa tgaatgtaaa agtatttagt attggtaata tacaaaattt
      661 tgataaagaa atttcatcaa aagatgaagg tcaaagaata tataaaaaat tatttatgaa
      721 agatggttca ttagttggag ctatacttat aaatgattta agctgtactg ttaaattgat
      781 aagattaata tctgaaaaag gtgattttga ggatattatg aaggctgata tactataatt
      841 taaatacatt aaaataaaaa ggagttgtct taaaattgat ttttttctaa tcaaatttta
      901 aattcaactc cttttttgaa tttttatata aaataacatg gaaattttag taatgtctgt
      961 ggttacatac ataggtttaa ttgtttttta tatggattgt ctctggtata ataaattgaa
     1021 ttgtgtttga ctgatagata tttttattga aagttaatac ctgttatgcg ataataagga
     1081 cgtgttaggg gagtttttat aataacaatt aatctcagtt tttgtgttcc aatagtagca
     1141 tcaacacatt ctgatgtagt agttcttttc ttataaaaat tagttacaaa tatgcttgta
     1201 attatttgat atgtaaattt accaatatta taaatgatga cctcgataat aaggagagtg
     1261 aaaaataatc tatgaacgaa aatattttga ttgtggatga tgaaaaagaa atagcagatt
     1321 taattgaagt atatttaaaa aatgaaggtt ttacagtata caagttttat acaggtaagg
     1381 aagctctaga atgtattgaa tctaaaaatt tggatatggc tattttagat attatgcttc
     1441 ctgatattga tggatttcat atttgccaga aaattcgaga aaaatactat tatcctatca
     1501 ttatgttaac agctaaaatt gaagatatag acaagataat gggtttaacc ataggtgctg
     1561 atgattatat aacaaagcca tttaaccctt tggaagttgt tgcaagagta aagacacaat
     1621 taagaagata tatgcgatat aataattctt atgaacaaca gtcgataatt gttaatgagt
     1681 atgatattaa aggattgatt atcaataaag aaacacataa atgtagttta tttgggaaag
     1741 aagtagcatt aacaccgatt gaattttcta ttctttggta tttatgtgaa catcaaggaa
     1801 aagtggttcc ttctgaagaa ttgtttgaag ctgtatgggg agaaaaatat cttgataata
     1861 ataacacagt tatggctcat atagggcgat tacgtgaaaa aattcatgaa ccttctaaaa
     1921 acccgaaatt tataaaaact gtatggggag taggttatac aattgaaaag taaaaatatt
     1981 aaagtcaatc ctgactttac tcaagtgaga aataaaattt ttatgagaac agctttaatg
     2041 gcatttatag ccattatttt catatggatt ttatattctt ttatattgta tggtaagttt
     2101 tctatctgga tagtatcatt ctttgaaaat gcttttcgta tggaatataa agctgcattg
     2161 aatttatatc agcagatttt ccgtaatagc atggagctct tcatgatact agctatggtg
     2221 attgtgtttt ttataatatt ccgtgtttat ttaaattggt ttacaaaata ttttagtgaa
     2281 ataaatcaag gtattgactc tcttatcaaa gaagatgttg gagaagtggc tttatctcca
     2341 gagttattgg caatagaaaa gaaaataaac tcaattaaac atatacttga acaacgtaaa
     2401 tttgagacac aaatggcaga acaacgtaag aatgaactta ttgtttatct tgcacatgat
     2461 ttaaaaacac cacttacatc agttataggg tatctgacac tacttcgaga tgaaagtcag
     2521 atttctgaag aactgcgaaa aaaatattta tctatttcgt tggataaggc agagcgtctt
     2581 gaagatttaa ttaatgaatt ctttgaaatt acaaggttta atttatcaaa tatatcactt
     2641 gaatacagta cagtaagctt aactcgtatg cttgaacaac taacatatga gtttaaacct
     2701 actttgatga gtaagaattt aaaatataca ctgaatgtta caccaaatat gatgataagg
     2761 tgtgatgtta ataaaatgca acgtgtattt gataacttat tacgaaatgc tgtttattat
     2821 agttttgaag atactactat tgaaataaca gctgtacagg aggatgatta tataaagctt
     2881 aaatttatga atcatagcaa tacgattcca gaggaaaaac ttgagcgtat ttttgaacaa
     2941 ttttacaggt tggatacatc tcgtaattca aacagtggta gagctgggtt gggacttgct
     3001 atagctaaag aaatagtgga gcttcataac gggacaataa cagctcatag cgagaacaat
     3061 ataattacct ttaaggttat tattccgatt gtaggaaatt tgtaagaatt taagtataaa
     3121 aaaataggaa attctttagg ataaataaat aaagagcact cttgttattg atataattac
     3181 tgtatcaaaa caagagtgct tttattttga tacagtaatt atatcaatag aaaaatgtga
     3241 aaatatatta tgtggaacag atgcaattgt aagtgaaggt tgattggatt tgaaatagaa
     3301 aaagtatttt aacatgtttc tgtagattgt ataaaaataa ataaatcaac aaaggagaat
     3361 caaagatgaa taataaaaaa attgcagtta tatttggtgg aaactcaaca gaatatgatg
     3421 tatcattaca atcagctttt gcagtacttg agaatttgaa tacggaaaaa tatgatatta
     3481 taccaattgg aattacaagg gagggtaatt ggtatcatta tattggagat tataataata
     3541 tccagaataa cacttggttt gaaagggata ctgatataat tccagttatt gtttcgcaga
     3601 accgtgtcaa aaatggtatt attgaatttt taattcctga tgctgtttct cagaatcata
     3661 ttccaaatag tgctattgaa tcatgtgaaa atgatattaa atacactaaa cttgattttg
     3721 tgtttcctgt actgcatgga aaaaatggag aagatggaac agtgcagggg ttatttgaat
     3781 tggcaggtat acaggttatt ggatgtggta ctctttcttc agcattatgt atggacaaag
     3841 acaaatcaca taaacttgtt cattcagaag gtattaaagt accaaaatca atattactaa
     3901 aatactcaga taaatttaat gtggctgaac tgtcaaaaga cttaacttat ccattattta
     3961 taaaacctgt gcgtgcaggt tcatcatttg gtattagtaa aatttataaa aaagaagagt
     4021 tacaagtagc agtagacttg gcttttgaac atgataatga agtaataatt gaggaaaata
     4081 ttgatggttt tgaggtaggg tgtgcagttt taggaaacga tgacttactt gttggaagag
     4141 tagatgaaat agaattgtca aaaggatttt ttgattatac agaaaaatat acattgaaaa
     4201 gttcaaaaat tcatatgcca gctagaattg atttggattg tgagaaaaga atacaagaga
     4261 cagcaaaaat tatttataga actcttggat gttctggatt tgctagagta gatatgtttt
     4321 taactccatc tggtgaaatt atttttaatg aagtcaatac aattcctggc tttacttctc
     4381 atagtcgtta tccaaatatg atgaaaggta ttggactgtc ttttgaagaa atattggaca
     4441 agataattga ggtgtacata aaatgatgaa taatatttta ttagataaaa ataaagaaga
     4501 taaaatgaca aatactatct tattgactaa agataaagtt tatgatggga atctaattct
     4561 agtaaatgct tttttaccag ttaaaacatc agaagatata gatttaattc ctgtagatac
     4621 aaggtttcca agtattctta tgaaacgtga agctacaaac attttacaaa atattttaaa
     4681 aagtatatgt ggaataaatg aaatagtacc tgtaagtgga tatcgtactg cagaggaaca
     4741 acaagatata tattcaagtt ctctgcgtga caatggaaaa gactttacta aaaagtttgt
     4801 agcacttcca aatcacagtg aacatcagac agggcttgct attgatttag ggttaaagaa
     4861 agaagttata gattttatct gccctgaatt tccttataat ggtatttgta atgagtttcg
     4921 taaggcagca ctacattatg gttttataga gcgttatcag tgtaaaaaag aagaaataac
     4981 aggaatatct caagaaccat ggcattttcg ttatgtggga tatccgcatt ctgagattat
     5041 caataaaatg aatctcgctc ttgaagaata tatagaatat attaagagat tttcgtatac
     5101 aaattgttta aaaatagaag ataaagaaaa aattatagaa atcttttatg tgcctatcca
     5161 tactaaagaa gaagtgagaa tctctatacc tgaacaatgt gtatatcaaa tatcgggtaa
     5221 caataaggat ggttttattg tgacattttg gagaaataga aaggactaag aaatggagga
     5281 acaagaatga ttaggaataa atcttatgca gggcttgatt attttaggat tatagctgca
     5341 ttacttataa ttgcaattca tacatctcca ttattatcat atagtaaaat ggctgacttt
     5401 gtgttaacta gtataattgc tcgtattgct gtgccattct tttttatgct atcaggcttt
     5461 tttctaattt caacatataa ttataattgt aataaactga aatcttttct aaaaaatgta
     5521 ttatttattt acgggatagc tattgtaatt tatattccct taaatattta cactggatat
     5581 ttttccatga ataatttagt gcccaatatt attaaggaca ttgtatttga tgggacttta
     5641 tatcatttat ggtatctacc cgcatcaatt attggtgcaa gtattgcata ctttcttgta
     5701 aaaaaactag gtttaaagta ctcactagta atcactattg ttatttatgt aattggtatg
     5761 tttggtgata gttattatgg ttttttagaa aaaataccac tattaaagaa tttatatggg
     5821 tatatttttg aagtatcaga ttatacacga aatggtatat tttttgcacc tgtatttttc
     5881 atacttggtg gaataatatc taataaatct aattatatgt caactaaaaa tagcttgatt
     5941 ggatttctta tatcattctt acttatgtta tgtgagggaa tgtttttaca taaactaggg
     6001 gtacagcgtc atgatagcat gtatattatg ttattgccat gtatgtattt cttatttaca
     6061 gccttaacat ttttgaaagg ttctcgagct aggttattgc gaacatcatc attaattatt
     6121 tatattatac atcctatgat gattgttgtt attcgtatgt tttctaaaat attcggaata
     6181 caaactgtct tggttgacaa tagccttatt cattatatag tggtaagtat agcatctttt
     6241 atagcttcta taattattat attgattctt aaacatctaa aaaaagactt gaaaggagaa
     6301 cataaggtaa ataccaatag agcttggatt gaaattgatt taaataattt ggaacataat
     6361 gtaaatatga tacaaaaagc tatgccagaa gattgtgaat tgatggcagt tgtaaaagct
     6421 aatgcctatg gtcatagtgc ttttgaagta gcgacttgtg caaaccaaat aggtgtaaaa
     6481 gcatttgcag tagcaacaat agatgagggg attgatttac gtcgatatgg aatactagga
     6541 gaaatattga ttcttggata tactaaccca ataagggcaa aggaacttca taaatacaat
     6601 cttacacaga gtgttattga ccttaatcat gctatctctt taaacaaaca gaaatacaac
     6661 ataaaatcac acattaaaat tgatacaggt atgcacagac tgggttttga tgttaatgat
     6721 gtgaggtcaa ttttaaaggc atttaaatta aaatacttag atatatgtgg catatttaca
     6781 cacttgtgtg tttcagatag tctgaaagat gaagatgtag actttacaaa taaacaaatt
     6841 gaatgttttt ataagttaat tgattttctg ttaaaaaaag gtataaaaat tcctaaagta
     6901 catattcaaa gtagttatgg tttttttaat tatcccaatc ttaagtgtaa ttatgtaaga
     6961 attggaattg cattatacgg gatattaagt tctccaaatg atacgactaa attgcaactt
     7021 gatttacgac ctgttttatc cttaaaatca caagtaattc ttattagaaa aatacaaaaa
     7081 gatgatagtt ttggatatgg aagagtcttt attgcaagca gagatagcgt aattgctata
     7141 ttatctattg gctatgccga tggtatacct agaaatttat cctgtggaaa aagttacgta
     7201 ataataaatg attgtagagc agcaatagtg ggtcgaattt gcatggacca acttgctgtt
     7261 gatattacag atatacctaa tgtagaagtt ggtaatacgg caataataat aggaagagat
     7321 aatttagctg aattatctgc ttctgaagtt gcaaataact catgtagtat atcaaatgaa
     7381 ttattgagca gagtaggaag aaggttaaat gttataaaga agtaactcaa ttaaatagta
     7441 atatttgtta tactgtgtaa tttattttac tctgactaga catttttagt aatattatgt
     7501 ctaatatata ttttttataa cttacatgac aatttaagtt ataaaaaggt gatgtttcag
     7561 atttttattt tgaaacatca ccttttttgt tataatatat aataaaataa ttatacctta
     7621 actgtaaact tatatagatt atttactttt taatattaga ttcatttttt gagattggta
     7681 tataataaac atatagagta tataataata gttaaagtta aaaaagggga aaagcaatat
     7741 gcagtatata caagaactac ttgatttgac tcaaaattat tgggtgttag ccacaataat
     7801 cggcttatta agtgcattta tagaaagctt tataccagcc ttaccacttg ttgctatagt
     7861 tacagcaaat gctgcaatac aaggattatt tattggatgt ttgttatcat ggattggctc
     7921 aggacttggg acaacttcat tattcttatt aataagcaga tttaatgata gtaagctttt
     7981 taataagttg agaaatagta aaactgaaaa agcaataagt tggatggata aacagggatt
     8041 taagttatta tttatagcgt atgcctgtcc atttatgcct ggttgtttag ttactatcgc
     8101 atcagctttt ggtaaaaaag atttaaaaga ttttgtgcca gcaatgttgg caggaaaatt
     8161 tgtaatgttt atagttataa gttacgtggc aagtgatata gaaggtttta taacaagtcc
     8221 tttaaagata gcatcattta tattattggt gtttttgtca tggaagattg gaaatagagt
     8281 aaataaaaat cttgaaaatc ataattatga tttccatcat aaaaaacatc acaatgataa
     8341 agatgataaa atgatttagc ttgtacgaaa taaaacgtat cttaaaatga tttagtacta
     8401 aattatttta agatacgttt tattttttgt attaaggtac aaaattttta gttcttttgt
     8461 aaactaaaat atataaaact tatttatact tagttcaaaa tattgttatt tattttttct
     8521 acaactataa taaaatataa aattgatatc caatcaaaaa aataactatg aaatttaata
     8581 gcttagatat gaatttttct gtttttgagt tagtttttat aggtagtaaa gaaatgttta
     8641 tggtaatggg ataaaacaat tcaatacctt ctttagtaat aaggtcaaga cacaagtgag
     8701 atagatatcc tattataaac ccactggaca aacatgagaa aactatatta ttgttagtga
     8761 agattgtcag agatttaaaa aaactataaa taagtaataa ggcaattaga ctgtgagtaa
     8821 accctctatg tctaaaccta cttccaaata atttgtatat taacataaat tttttactta
     8881 tgtaagaacc tctcatatct atatctggaa ataaagaacc taaatatgca aaataaatat
     8941 aaattataat taatactatt ttatatgcaa tatcgtattt aactaggtac agattgttta
     9001 taaaggggag tatcaaaagt gcgaatatat aacctcccct ttcatgtgtt tcttttatca
     9061 tataaaaatc ctcctgaatc gtcactataa acattatatt ggtaaattta gactatcttt
     9121 tattatatta tatagcaaac ataagttttt ttcaaaagga ttttaatatt taattaatct
     9181 tgagatttta aatttttgta attttctaaa gcaccattcc aatttactac attaaaccag
     9241 ttgtcaatgt attcatttct tctattttga tattttaaat agtaagcatg ctcccaaaca
     9301 tcaagtccta ttataggagt taggttttta cttacagggc tatcctgatt tggagtagtc
     9361 ataatagata atttgccatc tttagtagct ataagccaag cccaaccaga accaaagaca
     9421 tctaaagcag atttttggaa ctcttgctta aatttttcaa aagaaccaaa gtctctatca
     9481 atagcttctt ttaaagattc agaaggtatg gttttttctg gtgtcattat atcaaaaaag
     9541 aatttatgat tataagctcc acctgcatta tttcttacag ttgtagcaat atctttaggt
     9601 aaagaatcta aattttgcaa taattcacat aaagaataat tataaagctc aggatatttt
     9661 tcaagagcag catttaattt atcaacataa gcttgataat gcttatcatg atgcagtttc
     9721 attgtttctt tatctatata aggttcaagt gcatcatatg cataaggtaa tggtttaacc
     9781 ttaaatttgt tattttcagg tgtaaaagca aatgaagtta tgcactgtga gattataaat
     9841 aaagacataa taactggtat taatattttt ttcttcataa aaatcacctc ctaatatagt
     9901 atttctagaa ataaaaaaaa tatgaaatat taacagataa tttataagaa tttaatagca
     9961 atttcatttt aaattgaata agttttaaag ccaaagacaa aactatgaaa tatagaataa
    10021 gactaaagga cagaaaggaa ttttgattat atggacggca atatttcaat ttattataaa
    10081 ggtgatatat ctaaaaaaga agatattaaa tttaaaataa aagaatctgt aaataataca
    10141 agactttcta gtagaataga gtataaggat gaggaaaaaa cttttgattt tgaaagattt
    10201 aatgtaattg atgctattta tggaaataaa ccatttgaaa aagatggaaa agttattgtt
    10261 tttaatggaa atatatataa ctatcatgaa ataaaagaag aattaattaa aaaaggacac
    10321 aattttacta caaatgatga tattgaagtc ttattagcat cttatataga atttggtaag
    10381 aattgtgtac ataagattaa aggaatgttc aactttataa tctatgatag agaaaatgaa
    10441 agtatatttg gagcaagaga tttatttggg ataaaaccac tttattatat aaataaggaa
    10501 aatgcaataa ttttttcctc agagtataag tttttacttg aatatatgaa aaatttaaat
    10561 ataaatgaaa gaagcttaca aagttatttt tcatttcaat atgtacttcc tgaagatact
    10621 atgatac
//
LOCUS       DAELNY010000355        10042 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.guided.137, whole genome shotgun sequence.
ACCESSION   DAELNY010000355 DAELNY010000000
VERSION     DAELNY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.guided.137"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            77..754
                     /locus_tag="KPE55_003728"
     CDS             77..754
                     /locus_tag="KPE55_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494708.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(1087..1854)
                     /locus_tag="KPE55_003729"
     CDS             complement(1087..1854)
                     /locus_tag="KPE55_003729"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0494709.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2525..3340)
                     /locus_tag="KPE55_003730"
                     /pseudo
     CDS             complement(2525..3340)
                     /locus_tag="KPE55_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3432..4100
                     /locus_tag="KPE55_003731"
                     /pseudo
     CDS             3432..4100
                     /locus_tag="KPE55_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4227..4847
                     /locus_tag="KPE55_003732"
     CDS             4227..4847
                     /locus_tag="KPE55_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0494710.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            5003..5785
                     /locus_tag="KPE55_003733"
     CDS             5003..5785
                     /locus_tag="KPE55_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG0494711.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            5910..6836
                     /locus_tag="KPE55_003734"
     CDS             5910..6836
                     /locus_tag="KPE55_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0494712.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            6850..7288
                     /locus_tag="KPE55_003735"
                     /pseudo
     CDS             6850..7288
                     /locus_tag="KPE55_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            7649..8056
                     /locus_tag="KPE55_003736"
     CDS             7649..8056
                     /locus_tag="KPE55_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0494713.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            8058..8288
                     /locus_tag="KPE55_003737"
     CDS             8058..8288
                     /locus_tag="KPE55_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0494714.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            8678..8881
                     /locus_tag="KPE55_003738"
                     /pseudo
     CDS             8678..8881
                     /locus_tag="KPE55_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            8959..9312
                     /locus_tag="KPE55_003739"
     CDS             8959..9312
                     /locus_tag="KPE55_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG0494715.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            9384..10016
                     /locus_tag="KPE55_003740"
     CDS             9384..10016
                     /locus_tag="KPE55_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0494716.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         3628 a         1460 c         1592 g         3362 t
ORIGIN      
        1 gtcttatagt gctaatgagt actattagac catgttctat ggccttattt taatataaaa
       61 cgattggaga taggctatga ttgtgattga aggtagcgat aaatttaaga ttgcaaaaga
      121 atatattgat gtagaatata ctctttttag caaagtaact tttaggtatg aaaagttgaa
      181 atttaaagat aatgctgaat tggaaaaaat taagatgttt aaatataaaa atggctacat
      241 ccctaataaa ttaaaccttt cttttggata tggattctct tcttataaaa agcaaataat
      301 tagagaaact gtagatactt taagattgac agaaattttt tcaagcgaga acatagaaga
      361 tattaaattt ataaaagatg gtacaaaaaa attagaaatt agcatagaga aagttgtgaa
      421 atttaaacgt cgaaaaaaaa gaaattatgt ttgttgctat tgccctgata tgtataggga
      481 cataaaactc gacaaagaat ctatcaataa gatatacaac agaaaaataa aaatagaaag
      541 agaagttaat atttttgaag atgaggatgt tataataaac aaaaaagtat tgaagtttcc
      601 aaagtcttgg acaaagaata tgcaaaaata ttggttaagt gaaaataagt atcccataca
      661 ttctactgta attgatgatg atagatataa atgttgtaat gtacaatata caaaaaatag
      721 agtgataata ttatattaca tatataacca ttaatataca taattacaaa aaaataatga
      781 caaatataca taatacaaat atatcttcta ctttagataa aacagttatt atgaaaactt
      841 actatctata aataatagaa atgctagtat accatctata atctaatgtc tagtaggttt
      901 taatggcata ttttaaatat gccatagttg atttataaga ttttctatat cactttcaaa
      961 atacacacta aatagttgct tttcagatat aattgcccaa tttaaatttt actatctgaa
     1021 aagtttttta tatttaattt atatcttcaa aaataaaata ccttctaata tttctatata
     1081 attaatttaa ttaatactct tatgtaatag attaagtact gaatttaact ctttaaatga
     1141 tatttgtcct ggtgctgaaa cactttttgc agctccaaaa gtcaatgcag aaccaaatat
     1201 ttcaccacac aatctactta taacacccat tcctgacatt gacattgtta ttattggtct
     1261 atctgcataa attttaaaca tttcatttgt agcttcaagt agcactaaca catctttttc
     1321 attttgcggc ataacagcta ttttgggtaa atctgcgccc aattcctgca ttctacataa
     1381 acgagataca atctcttctt tttttggagt tttattaaaa tcatgattag atataattac
     1441 tttcacttct tttttatgag caaaattaac tacttcatct atgacctcat ctcccataaa
     1501 taactctaca tctattaaat ctactaaacc tgtattagaa atttctttat tcaaagtagt
     1561 ataataatct cttgatatta atttttctcc cccttctact acacttctaa atgtgaataa
     1621 taaagggata tcatgtatat aacttcttaa ttcatagaga acttccttaa cttctttaat
     1681 attttctaca ttctcaaaaa aatcaactcg ccactctata atatccaaac atgcatcttt
     1741 taactcttta gcctctttta taatatcttt tttatttttc ccaataattg gtacacaaat
     1801 tttaggtcta ccttctccaa tagtaatatt ttttacttga acttttcttt tcatattctc
     1861 ctccataatc agaaattcta taggacttta aattctattt tctgcacaat ttaattatac
     1921 attaaaaata ctatacatta taaaaatact aatttaaaaa taatttttat tctaattctt
     1981 aaaacaatcc cacatatttt agaaaaggta ctctataata tacaaacact tttttttacc
     2041 taaatttatt ttaacattct ataattttca caatattctt taaattttac tcatataaac
     2101 aatattattc tggatgctaa tagaattaat ctataaatca tagtataatt atgcagaaat
     2161 aactcgaatt tcactactta tgaagagatt gaatattaat gaatctaaat catttgcatt
     2221 atttaagagt tttgactaaa gttgaacacc atacaaaagt agctactcaa ttgtttatac
     2281 cccaaccaag cttaagtaat gttatataga ttctctaaaa agaatttagt atctttttat
     2341 ttgaaaaact tatattaaat ttgattatct tttcataagt tggtaaatgt cgatatatcc
     2401 tgtatctaca aggagtatcg acattttctt tttggaagat actttgcata agttggcatt
     2461 taaccgtata agaatgccat caaaagtagt aaataagtag taaactctag tcatactact
     2521 aggattataa ccgttccatt tcagctctcg cagaactaaa tgaaccatgt gcataatagc
     2581 caagcgtcat agtaatgttt ttatgcccca taatatattg caatatatta gtactcattc
     2641 ctttgttagc catatttgta cagagtgtat gcctaaaggt atgtggcgta atctttggta
     2701 gcttgtcctc atgggtcttg ttatacttct tgactaacgc tcttaacatg ctttgataat
     2761 gagttgcttg ttttggtaat ccatcttgtt taagaaatag gaagttctgg taaccatcaa
     2821 caatgattgg ttctgcttta gcgttcttac gccgttcaac gatacgttca aatgattgat
     2881 aagctctttc agtcattgga atttctcgct taccctgtgt ggtctttgga acttctacat
     2941 aatagccaat cttagtatca ctcaacaatt gatggtctac cataatggct ttatttttca
     3001 tatctaattc taaagtcaaa ccgcataatt cagaaatgcg tagtcctgtt tctaagagga
     3061 taacaacatc atcataatat ttataataag tcttatcgct ccccataaag gataataagc
     3121 ccatttcctg ttctggtgtt agagcgattt ttggttctgt tgtatcttcc aaaacatcac
     3181 ttaactaaaa attaaatgga ttctttctaa tacagtcgtc ttgaattgcc atgaaaaacg
     3241 acgctttcag tgagcgctta taattgtcaa tggatttata agcacaccca tcttcactca
     3301 ttcggatagc ccagctttta gcgtctgata atttaaccat atcaatgctt tttgcaccta
     3361 gtgggtcatt gatagtatct atattttgat ttataattta tacatttttt taaaaagaga
     3421 cttttaactg taaagaatct atgtataaac ttagctatgt tgtccgtgta tctacaaagg
     3481 atcttgacga gctaaaaagg cgttgtgatg aagtcaaaga cttttacgac gacttatcaa
     3541 tcaagctcgt gcgtcccttt ggggatatgt tgggattgca tggcgaattt attcctgtca
     3601 gcaaacgtta catcaatgat tatattcaat atgtcacttc ggattttttg gctggacttg
     3661 gttttggagc gacacaaacc cttggcgaac atgaggatat ctatcttggc tacaatctgg
     3721 acactggaaa gaatgtctat ctaaaacctg cccttgctag tcaaggggta aagggttcta
     3781 ttaccaatgc gttagcgtct gcgtttatcg gttcattggg tggtggtaaa ttattcagta
     3841 acaatatgct tgtttactat gcggtactat tcggcggaca agcggtcata gttgacccta
     3901 aagcaaaacg cggcaaatgg aaagaaacgc taccagaaat agcccatgag ataaacattg
     3961 tcaatttaac cagtgatgat gaaaacaaag gattgcttga ccattacgtc attttgagcc
     4021 gtaaaaagga ttctgaaagt ttagcgattg atatcctaac attccttact ggtatctcca
     4081 gtcgtgacag tgataagttt ttgcagaaaa atgacaactg gaaaataata atggaatagt
     4141 ttctatgtaa atatattttt actatcttac ctaactaatt gaaagatgat ataatttaat
     4201 caaagatttg gtaaggagag atgaatatgc atttaattga ttttatgaat gaagatatta
     4261 aaaagaagct tgttaaaaaa acgtataaaa agaaagagac tattcttttt gctgagcaag
     4321 aaaatgaata tgtaattctt atgattgacg gtattgcaga agcattcatc ttaaatgtga
     4381 aaggaaacat ctcaactatt catctttata gaagtggtag tttttttgga gaaatggaac
     4441 aatttaatga tgggaaaaaa cccgttgaaa ttgtcgcttt cacagattgt aatgtatata
     4501 aacttcatag agatgatttt ttaaactggt taaggggaga ttttgaagct acaaaatttc
     4561 tgattcgaga aatatctagt aaattagttg taaatgctga attaattgaa gaattatcat
     4621 ctttaagtgt taaagaacga cttctgaggt gtatatcctt acactattat agaagtgaat
     4681 taaatctgct taccaaaaaa cagcttgcta gcgaagtaaa cgctccgatt agaagcatta
     4741 atcgtgctat aaatgaatgt gtacaagaac atctaattgc ttatgaaaac aaaagattca
     4801 gtgtaattaa tcagcgagag gtattaaaga atcttcctac aacataagta taatttcaaa
     4861 aaagtattgg ataataatta ttcaatactt ttttatttcg ggtcaagtga cctatttttt
     4921 tttatttctt cattatataa tactcctatg cataattatg ttggattgat agctaataaa
     4981 agacagtgag aggtgttata ttatgactaa aacacaacag gaaatgatac aaaacgtatt
     5041 agaattttca aaaagagata gtaggataat aggacttgca attagcggtt cttatatcac
     5101 aaaatcgtta gatgaatatt ctgatttgga ttttctgatt gtagcagatg ataaatccta
     5161 tgaagaagtt atcgaagaga gattacttat tgtggaacag tttggaacat tagtttctgc
     5221 ttttactggc gaacatgttg gagaacctcg attgataatt tccctctatg attcccctgt
     5281 tcttcatgtt gattttaagt ttacaacctt agatggttta ctagataggg ttgaagattc
     5341 agctattctc tatgaagaaa acaactgcat aacagaaatt tattctaaaa aagaagcata
     5401 cttcccaact ccagacttac agtggattga agatagattt tggatttggg ttcattacac
     5461 ttgcactaag attggacgta gagaactatt tgaaacaatt gattttttat cttttttaag
     5521 acaatcagta cttgcctcgt taatacagct tctcttaggt aaattacctc gtggagttag
     5581 aaaaattgaa ttggacgcac cccaatatat tgaagcatta gaaaagacgg ttgctaacca
     5641 tgaatataaa agctgtgtta atgcattgca ggctactatc aacctgtatc ttgaattaag
     5701 agaggaatta tcacaaagtc ctattgtccg aaaggttgaa gctgaaaggg ttgcattaca
     5761 gtattttcaa gatttaatca aataaaccca aagtaaaaaa atattgaata gtaaatattc
     5821 agtgtttttt catttttggt caaatgacct agattagttg ttgtaacaat tctataatta
     5881 ttttgaaagt aacagtatgg aggataacta tgagtgatat tttaagtaga attacaaata
     5941 aaattgggga taagaatata gttgataagt tatcagctct atcaaaatca gatttgaact
     6001 cacttctatt agaagtgttt gatagacaag caaatacttt aactgcaact gatattctaa
     6061 agtcatacca actaaataga tttactatcc ctagcggtat agatccagaa gaaattcatg
     6121 ctttggagtc aaaattattg agaaaagcat tcaatatgga tatcaagacg attatgcttt
     6181 ctccctctgc tccattaggt agctgtagcg tttttggctc cgtagatcaa tataatgtag
     6241 taagtgctct acgagggaca gaaacacttg cagacccctc aaatatgttg gcaataatta
     6301 ttgctgataa attaaaaaga aaagaagaaa ataataccat acctatccac atggctacta
     6361 ctgctagggt aataagagca caaaattttg taggtaaagg tttatattca cattttggct
     6421 tatattgtat ggtttcatct ggtatagata cagggtctta cacttgtgaa aaaatgttgt
     6481 tagaaaagca cttaaactat tataaagata tattaagtga aatagaaaat gttcgtttct
     6541 ctattattct acgcaaaaga aacggataca aagataatga cggatttttc gacagaatgg
     6601 ttgaaactat caaacttatt tttcctaata tagagttatc aattcataat gatgatgttg
     6661 ataataatta ttataaaggt atcaatttca aattatcggt caaacaagga aatgaaaccg
     6721 ttgaaatagt tgacggtgga tttgttgact ggacttatca aatgcttgga aataaaaaag
     6781 aacgctgttt gataagtggt attggcttag aaagatttct tgtagctgtt tcataaaagg
     6841 agaagaaaaa tggaacttat agagtggaat tatgcttata ttaccgattt agttgagttt
     6901 gcgaatgata aagaaattgc aaacaatctt cgtgacaact tcccccaccc ttataggtag
     6961 caaaacgcta gagattttat tagtttttgt ttgacaactt cagatgttaa acaacttagc
     7021 tatgctattt tttatcaaga aagggctatt ggaagtatca gcctaacatt tgaagaagat
     7081 acctctatta ataaaaatgc tgaattaggt tactgacttg ctaggaacta ttggggcaaa
     7141 gggatagcaa caaatgcagt aaaagaaata tgcaaaattg cctttgaaaa atacaacctt
     7201 aacaaaattc atgctaatgt atattcctac aatagagagt caagtaaaat gtggctttaa
     7261 gattgaagaa tactgtcaca attcataata aagaatagaa acatgattga ttgccatgta
     7321 tatagtttaa ttcgaccgca atagcattta ttagaattaa cctacttttt aataacagaa
     7381 taagtttttc ccctctatta atttcttatg catctttgta tttttatgaa aagctagcca
     7441 atgcatattt ccatacatca atatggtggt ggcttacaac caatataaat taaatacgtt
     7501 ttaaagaata cacatttttg ccctataggc agagtgtgta tttttttctc tttttttttg
     7561 aaatttatcg aagatttaac gccctcccac cgtgatagga gtaagcaagg ttatttaact
     7621 ccttgcgatg gaaagagggt gaaatcttat ggttgatgaa aagacaattg aacatcaatt
     7681 cgacagtttc tgtaaaacag ttttgcgtaa ctatgctaga gatatttatg acgaaaataa
     7741 acgcagaaat gattatttgg tatctctcga atctttgtca cttgcagaat taagtaaatt
     7801 gagtatttta gatgattatg attctaacta catctgtatg gtttcttacg actacaacat
     7861 acgaattgaa gatgtattaa tggcacaagc aatcggaaaa cttacaaaaa gaaaacaaga
     7921 tataattcta ctttcattct ttctcaacat gacaaatgct gacatagcaa cattgatgga
     7981 tttagcagaa aataccatac attatcacaa aacaaatgca ctaaaagagt tgaaagaact
     8041 catgcaggaa cattgaaatg aaacctacta aagaagtatt accttttaca attattttac
     8101 ttgctgtaca tggcgatgta aatgcaataa acgtaatact taaacatttt gaacactata
     8161 tcattcgtct ttcacagaaa actttatttg atgaatacgg aaatccctat attcatgtag
     8221 aagaagaaac aaagcgaatg ctaggaacaa aactaatcac tgctattttg agatttaatt
     8281 tgtcttaatc tcgccctctc tccatgtatc aagctatttg gtacatggag aatcactaat
     8341 tatttgtcta taaaagccca taagctattg tgatttttta taggcaaatc aatgccgact
     8401 gttctttgac aactgaataa agtagataga tacgtttgtg atacagatag ccaacaaata
     8461 ctatacgcca tgaccttaat caagagagct actatatagt gatttgtgaa taacctttgg
     8521 aacggttgtt ttgccgtaac ctgtaccaca gtataatgat acacctgtat gacacggttc
     8581 tgcccataag aatgggattg gttgaaatac caatggagct taccaaagcc atctatctac
     8641 ttctgaacta cgaacataga aaggagtgat acattctatg aacaatactg atgtacctat
     8701 ttgggaaaaa tataccttga cgattgaata agcgtcaaaa tactttcgta ttggagagaa
     8761 aaaattacgt aaacttgctg atgaaaacct tgattctggc tgggtcatta tgaatagcaa
     8821 tcgcattcaa ataaagagaa aacaatttga gaaaatcgtt gatacacttg acgttatcta
     8881 atgaaattga gccttatatg ttataatatt catatgacgt atcaagctca attcggttat
     8941 tgagaggagc ttcttatcat gtcaactgaa aaaagacgag ataataaaga tagaatttta
     9001 agaactggag agagccagag aaaagatgga agatacgcat ataagtatgt atattcattt
     9061 ggtaaagcac aatttgttta ctcttggaaa cttgtaccta ctgataaacc cccaaaggga
     9121 aaacgtgatg atatatcatt aagggataag gaaaaagcaa tacaaaatga ccttgatgat
     9181 agcattgaca ctattggtaa gaaaatgaca gtttgtcagt tatacgctaa aaagaatgta
     9241 ctaagaaaga atatccgact taacactaaa aaaggacgcc attatcttat gaagattctt
     9301 aacaaggagt gattctgtga agagatgtat tgtagtagtg gaatagtcaa aaacataata
     9361 tatattaaag gagagattta aatatgacta ttccaagttc aaataaggta tatccaagaa
     9421 gtaatgatta tcaaactgta tatcttaaaa atgtaataac aagggaaaat atagaagttg
     9481 gagattacac aatatataat gacttttata atgacccaag agattttgag aaaaacaatg
     9541 tattgtatca ttaccctata aacaatgata aattaataat tggtaagttt tgctcaatag
     9601 catgtaatgc aaaatttcta atgactagtg gaaatcactc aatgaaatca aaatccacat
     9661 atacttttcc catcttttat gaagaatgga aactcgatat caaccatata acggacgctt
     9721 gggacaataa gggtaacatt ataattggaa atgatgtgtg gataggttat gattctataa
     9781 ttatgtcagg tgtaaatatt ggtgatggtg caattattgg aacaagagca gtagttacta
     9841 atgacgttcc accgtattct attgttggag gagtacctgc aaaaattata aaaaagagat
     9901 ttaataatga tacaatttca aaattattag aaattaaatg gtgggattgg ccatataaaa
     9961 aaattcaatc aaacattaag catattcaat caggaaatat tgataaatta gtttaatatt
    10021 ttataattta tttatattaa ac
//
LOCUS       DAELNY010000356         8024 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019483
            SAMN15907403-rid12314673.guided.170, whole genome shotgun sequence.
ACCESSION   DAELNY010000356 DAELNY010000000
VERSION     DAELNY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907403
            Sequence Read Archive: SRR12520629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050481.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:35:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,747
            CDSs (total)                      :: 3,663
            Genes (coding)                    :: 3,617
            CDSs (with protein)               :: 3,617
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 19 of 46
            Pseudo Genes (incomplete)         :: 20 of 46
            Pseudo Genes (internal stop)      :: 19 of 46
            Pseudo Genes (multiple problems)  :: 11 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907403-rid12314673.guided.170"
                     /isolate="M2016019483"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-28"
                     /collected_by="CDC, Emerging Infections Program"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KPE55_003741"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KPE55_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0494717.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="KPE55_003742"
     CDS             1575..2258
                     /locus_tag="KPE55_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0494718.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEIASLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="KPE55_003743"
     CDS             2255..4915
                     /locus_tag="KPE55_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0494719.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQDASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="KPE55_003744"
     CDS             complement(5096..6277)
                     /locus_tag="KPE55_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0494720.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="KPE55_003745"
     CDS             complement(6277..6939)
                     /locus_tag="KPE55_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0494721.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(7071..7709)
                     /locus_tag="KPE55_003746"
     CDS             complement(7071..7709)
                     /locus_tag="KPE55_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0494722.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..>8024
                     /locus_tag="KPE55_003747"
     CDS             7888..>8024
                     /locus_tag="KPE55_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0494723.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KT"
BASE COUNT         2988 a         1006 c         1239 g         2791 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtatact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatttag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aagatattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaatt caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgataa ataaagttga
      781 tgctaaatat gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtc
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatagca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac tcttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga agatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatttatag accagctatt ttattagcag atgagccaac tggtaatctc gatagaaaaa
     2101 attcagaaga aattgtagat ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataaa
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 ggataaaatt actctcccaa taggagatag aatcctaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgccaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggt tgactgaaaa agatgcttca aaagaacttg cgaaaaatgg
     3901 aggatttaaa aatatagctt catctaaaaa gtactctcct atagaacgtg atggaaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtgcagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaag atgctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtatagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatac aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ttgtcctcca ttctatatcc
     6301 tatgcctctt aatgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggattgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttt atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcctta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatacttatg taaatataat acttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggga aaatataatt aaagaattaa aaac
//
